[Physico-chemical and immunobiological properties of the ribosomal preparations of Clostridium perfringens, type A].
Ribosomes, sufficiently purified by physicochemical criteria (with the RNA/protein ratio equal to 1.6 - 1.7), were isolated from disintegrated C.perfringens cells, type A, strain BP6K, by ultracentrifugation or by fractionation with polyethylene glycol. The purified conjugates of ribosomes and C.perfringens toxoid, absorbed on aluminum hydroxide, were obtained. The ribosomal preparations had low toxicity and high protective activity: when used in a dose of 6 binding units, they protected guinea pigs and mice inoculated with 8 - 9 LD50 of C.perfringens culture.
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D000818:Animals; D001428:Bacterial Vaccines / Q000302:isolation & purification; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003016:Clostridium perfringens / Q000648:ultrastructure*; D006168:Guinea Pigs; D007109:Immunity / Q000502:physiology*; D051379:Mice; D012270:Ribosomes / Q000737:chemistry* / Q000276:immunology; D014121:Toxoids / Q000302:isolation & purification*
D001428:Bacterial Vaccines; D014121:Toxoids
Fiziko-khimicheskie i immunobiologicheskie svoĭstva ribosomal'nykh preparatov Clostridium perfringens tipa A.