Application of free-flow electrophoresis for isolation and purification of proteins and peptides.
Free-flow electrophoresis (FFE) has been applied to the separation and purification of a variety of proteins and polypeptides: bee venom, tumor necrosis factor, interleukin-1beta, interferon-gamma and superoxide dismutase. FFE at constant pH and conductivity of the carrying buffer is shown to be efficient at various separation schemes. In some cases, the method allows us to obtain proteins with a purity of more than 90% at a productivity of 20-30 mg/h. An electrophoretic apparatus with a new, multi-sectional construction of the electrophoretic chamber and a system for cross-displacement of carrying buffer in the chamber is described.
Loseva
O I
OI
State Research Center for Applied Microbiology, Obolensk, Moscow Region, Russia.
Gavryushkin
A V
AV
Osipov
V V
VV
Vanyakin
E N
EN
D000818:Animals; D001030:Apamin / Q000302:isolation & purification; D001516:Bees / Q000737:chemistry; D002417:Cattle; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification; D007371:Interferon-gamma / Q000302:isolation & purification; D007375:Interleukin-1 / Q000302:isolation & purification; D008555:Melitten / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D010741:Phospholipases A / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D013482:Superoxide Dismutase / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000302:isolation & purification
D007375:Interleukin-1; D010455:Peptides; D011506:Proteins; D014409:Tumor Necrosis Factor-alpha; D008555:Melitten; D001030:Apamin; D007371:Interferon-gamma; D013482:Superoxide Dismutase; D010741:Phospholipases A; D006821:Hyaluronoglucosaminidase