title: Pancreatic beta-cell regeneration after 48-h glucose infusion in mildly diabetic rats is not correlated with functional improvement.
abstract: We investigated the effect of glucose infusion on beta-cell regeneration in rats made mildly diabetic by a single injection of low dosage (35 mg/kg) streptozotocin (STZ). Nondiabetic (ND) and STZ rats were submitted to a 48-h glucose infusion (hyperglycemia approximately 22 mmol/l in both groups: ND and STZ hyperglycemic-hyperinsulinemic [ND HG-HI and STZ HG-HI rats]). Before infusion, beta-cell mass was 65% lower in STZ rats than in ND rats (2.0 +/- 0.02 vs. 5.5 +/- 0.6 mg), 1.6-fold increased in ND HG-HI rats (8.7 +/- 1.7 mg), and 2.7-fold increased in STZ HG-HI rats (5.4 +/- 0.9 mg). In ND HG-HI rats, beta-cell enlargement was related to an increase in beta-cell responsiveness to nutrient secretagogues both in vivo and in vitro, whereas in STZ HG-HI rats, no significant improvement in insulin secretion could be noticed. To determine the respective role of hyperglycemia and hyperinsulinemia on beta-cell area changes, ND and STZ rats were submitted to a 48-h hyperinsulinemic-euglycemic clamp. No modification of beta-cell mass was detected in either group. In conclusion, 48-h superimposed hyperglycemia was enough to restore beta-cell mass previously reduced by STZ injection. This effect seemed to be due to hyperglycemia rather than hyperinsulinemia alone. The data stress the dissociation between beta-cell regeneration and improvement in islet function in diabetic rats. Our model seems suitable for studying factors that can improve the plasticity and function of the pancreas in NIDDM.
authors.affiliation: Laboratoire Physiopathologie de la Nutrition, Université Paris, France. cbernard@paris7.jussieu.fr
authors.forename: C
authors.initials: C
authors.lastname: Bernard
authors.forename: C
authors.initials: C
authors.lastname: Thibault
authors.forename: M F
authors.initials: MF
authors.lastname: Berthault
authors.forename: C
authors.initials: C
authors.lastname: Magnan
authors.forename: C
authors.initials: C
authors.lastname: Saulnier
authors.forename: B
authors.initials: B
authors.lastname: Portha
authors.forename: W F
authors.initials: WF
authors.lastname: Pralong
authors.forename: L
authors.initials: L
authors.lastname: Pénicaud
authors.forename: A
authors.initials: A
authors.lastname: Ktorza
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D005947:Glucose / Q000008:administration & dosage*; D006943:Hyperglycemia / Q000503:physiopathology; D006946:Hyperinsulinism / Q000503:physiopathology; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D007328:Insulin / Q000097:blood / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000473:pathology / Q000503:physiopathology*; D007930:Leucine / Q000494:pharmacology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration
chemical_list: D001786:Blood Glucose; D007328:Insulin; D001120:Arginine; D007930:Leucine; D005947:Glucose