Effects of luteinizing hormone (LH), PGE2, 8-Epi-PGE1, 8-Epi-PGF2 alpha, trichosanthin and pregnancy specific protein B (PSPB) on secretion of prostaglandin (PG) E (PGE) or F2 alpha (PGF2 alpha) in vitro by corpora lutea (CL) from nonpregnant and pregnant cows.
Both Day 14 corpora lutea (CL) of the estrous cycle and Day 200 CL of pregnancy secrete detectable prostaglandin E (PGE) and prostaglandin F2 alpha (PGF2 alpha) in vitro. Corpora lutea from Day 200 pregnant cows secrete more PGE and PGF alpha in vitro than Day 14 CL of the estrous cycle when incubated in control medium without treatments (p < or = 0.05). In addition, secretion of both PGE and PGF2 alpha in vitro by both Day 200 CL of pregnancy and Day 14 of the estrous cycle increase (p < or = 0.05) with time in culture in the absence of treatments. The PGE:PGF2 alpha ratio secreted at 4 h in the absence of treatments by Day 14 CL of the estrous cycle was 1.2 and at 8 h was 1.0 and did not differ (p > or = 0.05), while the PGE:PGF2 alpha ratio secreted by 200 day CL of pregnancy in the absence of treatments at 4 h was 0.8 and at 8 h decreased (p < or = 0.05) to 0.4. The PGE:PGF2 alpha ratio at 8 h by 200 day CL of pregnancy was lower (p < or = 0.05) than in the Day 14 CL of the estrous cycle at 4 or 8 h. Secretion of PGE or PGF2 alpha was affected by luteinizing hormone, PGE2, 8-Epi-PGE1, 8-Epi-PGE2, trichosanthin, and pregnancy specific protein B (PSPB) and was time and dose dependent (p < or = 0.05). In summary, the altered ratio of PGE:PGF2 alpha may explain the decreased secretion of progesterone at 8 h by Day 200 CL of pregnancy reported previously from the same samples. In addition, caution should be exercised in interpretation of progesterone secretion data with bovine CL studies in vitro. Also, PSPB may play an indirect role through PGE to regulate bovine luteal secretion of progesterone.
Weems
Y S
YS
Department of Animal Sciences, University of Hawaii, Honolulu 96822, USA.
Lammoglia
M A
MA
Vera-Avila
H R
HR
Randel
R D
RD
Sasser
R G
RG
Weems
C W
CW
D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000494:pharmacology; D002417:Cattle / Q000502:physiology*; D003338:Corpus Luteum / Q000502:physiology*; D015237:Dinoprost / Q000031:analogs & derivatives / Q000378:metabolism; D015232:Dinoprostone / Q000494:pharmacology; D004971:Estrus / Q000502:physiology*; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology; D011270:Pregnancy, Animal / Q000502:physiology*; D011458:Prostaglandins E / Q000378:metabolism / Q000494:pharmacology; D015978:Trichosanthin / Q000494:pharmacology
D011257:Pregnancy Proteins; D011458:Prostaglandins E; C075750:8-epi-prostaglandin F2alpha; D015978:Trichosanthin; D007986:Luteinizing Hormone; D015237:Dinoprost; D016282:Aspartic Acid Endopeptidases; C094766:pregnancy-associated glycoprotein 1; D015232:Dinoprostone