Assaying granulocyte phagocytosis by chemiluminescence: effect of storage time and temperature of blood samples.
The objective was to study the effect of storage of blood samples for up to 4.5 h at either room temperature or on ice on granulocyte phagocytosis as assessed by chemiluminscence. The study included three groups of animals: eight pigs, seven cows and eight horses, which were bled at one occasion. The blood samples were divided into two and stored either at room temperature or on ice. Granulocyte phagocytosis in the samples was then assessed every 60 min starting 30 min and finishing 4.5 h after bleeding. The phagocytosis was recorded as the AUC of chemiluminescence following phagocytosis of opsonized zymosan particles. The time of storage of blood samples had a significant (P < 0.001) overall effect on the chemiluminscence following phagocytosis in all species tested, as was the case with storage temperature (P < 0.001). It should be noted, however, that there were considerable species differences in the effect of the storage on the granulocyte phagocytosis. Hence, for valid comparisons of data on granulocyte phagocytosis assessed by chemiluminiscence it is important that the handling procedures of the blood samples have been applied consistently even if the assays have been performed only a few hours after blood collection.
Magnusson
U
U
Department of Obstetrics and Gynaecology, and Clinical Chemistry, Faculty of Veterinary Medicine, SLU, Uppsala, Sweden.
Holst
H
H
D000818:Animals; D019540:Area Under Curve; D001793:Blood Preservation / Q000592:standards / Q000662:veterinary*; D002417:Cattle / Q000097:blood* / Q000276:immunology; D005260:Female; D006098:Granulocytes / Q000276:immunology*; D006736:Horses / Q000097:blood* / Q000276:immunology; D007958:Leukocyte Count / Q000662:veterinary; D008163:Luminescent Measurements; D010587:Phagocytosis; D013552:Swine / Q000097:blood* / Q000276:immunology; D013696:Temperature; D013997:Time Factors