Polyhemoglobin-superoxide dismutase-catalase as a blood substitute with antioxidant properties.
Polyhemoglobin-superoxide dismutase-catalase is designed to function as an oxygen carrier with antioxidant properties. This is based on cross-linking hemoglobin with superoxide dismutase and catalase (PolyHb-SOD-CAT). This study describes the structural and antioxidant properties of this solution. Our studies show that superoxide dismutase and catalase retain their enzymatic activity following glutaraldehyde polymerization with 8:1 and 16:1 glutaraldehyde:hemoglobin ratio. We have analyzed the optimal SOD/CAT ratios to prevent oxidation of hemoglobin in the presence of oxygen free radicals. The circulation half-life of crosslinked hemoglobin, SOD, and catalase in Sprague-Dawley rats correlates with the degree of polymerization as determined by high-performance molecular weight gel filtration. PolyHb-SOD-CAT decreases the formation of oxygen radicals compared with PolyHb in a rat intestinal ischemia-reperfusion model.
D'Agnillo
F
F
Artificial Cells and Organs Research Centre, Faculty of Medicine, McGill University, Montreal, Canada.
Chang
T M
TM
D000818:Animals; D001802:Blood Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D002374:Catalase / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002850:Chromatography, Gel; D006207:Half-Life; D006454:Hemoglobins / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D007422:Intestines / Q000098:blood supply; D008297:Male; D008970:Molecular Weight; D010108:Oxyhemoglobins / Q000378:metabolism*; D015202:Protein Engineering; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000097:blood / Q000188:drug therapy; D013482:Superoxide Dismutase / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D013481:Superoxides / Q000737:chemistry
D001802:Blood Substitutes; D006454:Hemoglobins; D010108:Oxyhemoglobins; C037158:polyhemoglobin; D013481:Superoxides; D002374:Catalase; D013482:Superoxide Dismutase; C086134:polyhemoglobin-superoxide dismutase-catalase