Functional intertwining of Dpp and EGFR signaling during Drosophila endoderm induction.
Endoderm induction in Drosophila is mediated by the extracellular signals Decapentaplegic (Dpp) and Wingless (Wg). We discovered a secondary signal with a permissive role in this process, namely Vein, a neuregulin-like ligand that stimulates the epidermal growth factor receptor (EGFR) and Ras signaling. Dpp and Wg up-regulate vein expression in the midgut mesoderm in two regions overlapping the Dpp sources. Experiments based on lack of function and ectopic stimulation of Dpp and EGFR signaling show that these two pathways are functionally interdependent and that they synergize with each other, revealing functional intertwining. The transcriptional response elements for the Dpp signal in midgut enhancers from homeotic target genes are bipartite, comprising CRE sites as well as binding sites for the Dpp signal-transducing protein Mad. Of these sites, the CRE seems to function primarily in the response to Ras, the secondary signal of Dpp. We discuss the potential significance of why an inductive process might use a secondary signal whose function is intertwined with that of the primary signal.
Szüts
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Medical Research Council (MRC) Laboratory of Molecular Biology, Cambridge CB2 2QH, UK.
Eresh
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Bienz
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D000818:Animals; D001665:Binding Sites / Q000235:genetics; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004064:Digestive System / Q000196:embryology; D004330:Drosophila / Q000196:embryology* / Q000502:physiology; D029721:Drosophila Proteins; D004627:Embryonic Induction / Q000235:genetics / Q000502:physiology*; D004707:Endoderm / Q000502:physiology*; D004742:Enhancer Elements, Genetic / Q000502:physiology; D066246:ErbB Receptors / Q000235:genetics / Q000502:physiology*; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D020880:Neuregulins; D011518:Proto-Oncogene Proteins / Q000502:physiology; D012097:Repressor Proteins; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology*; D014157:Transcription Factors; D051155:Wnt1 Protein
D017362:Cyclic AMP Response Element-Binding Protein; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; C112506:MAD protein, Drosophila; D020880:Neuregulins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C066177:Ubx protein, Drosophila; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C059843:lab protein, Drosophila; C074622:wg protein, Drosophila; C102104:vn protein, Drosophila; D066246:ErbB Receptors