title: Endodontic pathogens stimulate monocyte chemoattractant protein-1 and interleukin-8 in mononuclear cells.
abstract: Microbial infection of the dental pulp leads to the recruitment of leukocytes and the formation of lesions of endodontic origin. The chemokines interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) are relatively specific chemoattractants for neutrophils and monocytes, respectively. In the present studies, peripheral blood mononuclear cells were stimulated by Streptococcus mutants, Porphyromonas endodontalis, and Peptostreptococcus anaerobius, which are associated with lesions of endodontic origin. Each of these bacteria induced a dose-dependent increase in IL-8 and MCP-1, determined by ELISA. The levels induced are physiologically relevant. However, low doses of P. endodontalis were less effective in inducing IL-8 or MCP-1 expression, compared with S. mutants or P. anaerobius. Thus, these bacteria can induce significant levels of the chemokines IL-8 and MCP-1, which could contribute to the recruitment of neutrophils or monocytes in vivo. The expression of these mediators may contribute to the development of endodontic infections, particularly with regard to inflammatory leukocyte recruitment.
authors.affiliation: Department of Endodontics, School of Dental Medicine, Boston University, MA, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Jiang
authors.forename: T R
authors.initials: TR
authors.lastname: Russell
authors.forename: H
authors.initials: H
authors.lastname: Schilder
authors.forename: D T
authors.initials: DT
authors.lastname: Graves
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D018375:Neutrophil Activation; D010462:Peptostreptococcus / Q000276:immunology / Q000502:physiology; D017861:Porphyromonas / Q000276:immunology / Q000502:physiology*; D011817:Rabbits; D013295:Streptococcus mutans / Q000276:immunology / Q000502:physiology*
chemical_list: D018932:Chemokine CCL2; D016209:Interleukin-8