Characterization of an unstable allele of the Arabidopsis HY4 locus.
The Arabidopsis HY4 gene encodes the nonessential blue light photoreceptor CRY1. Loss-of-function hy4 mutants have an elongated hypocotyl phenotype after germination under blue light. We previously analyzed 20 independent hy4 alleles produced by fast neutron mutagenesis. These alleles were grouped into two classes based on their genetic behavior and corresponding deletion size: (1) null hy4 alleles that were semidominant over wild type and contained small or moderate-sized deletions at HY4 and (2) null hy4 alleles that were recessive lethal and contained large HY4 deletions. Here we describe one additional fast neutron hy4 mutant, B144, that did not fall into either of these two classes. Mutant B144 was isolated as a heterozygote with an intermediate hy4 phenotype. One allele from this mutant, hy4-B144(Delta), contains a large deletion at HY4 and is recessive lethal. The other allele from this mutant, HY4-B144*, appears to be intact and functional but is unstable and spontaneously converts to a nonfunctional hy4 allele. In addition, HY4-B144* is lethal in homozygotes and suppresses local recombination. We discuss genetic and epigenetic mechanisms that may account for the unusual behavior of the HY4-B144* allele.
Bruggemann
E P
EP
Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-5430, USA.
Doan
B
B
Handwerger
K
K
Storz
G
G
D000483:Alleles; D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins / Q000096:biosynthesis / Q000235:genetics*; D017343:Genes, Plant; D005838:Genotype; D008027:Light; D016296:Mutagenesis; D009502:Neutrons; D010641:Phenotype; D017956:Photoreceptor Cells, Invertebrate; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; C538963:CRY1 protein, human; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; D010940:Plant Proteins; D043562:Receptors, G-Protein-Coupled; C119828:cry protein, Drosophila