Overexpression of hMTH1 mRNA: a molecular marker of oxidative stress in lung cancer cells.
Human MutT homologue (hMTH1) mRNA was overexpressed in SV-40-transformed non-tumorigenic human bronchial epithelial cells (BEAS-2B cells) and in 11 out of 12 human lung cancer cell lines relative to normal human bronchial epithelial cells. Expression levels of hMTH1 mRNA were inversely proportional to cellular levels of 8-oxo-deoxyguanosine. Together, these results suggest that hMTH1 gene expression may represent a molecular marker of oxidative stress that could ultimately be used to elucidate the temporal relationships between oxidative stress, genomic instability and the development of lung cancer.
Kennedy
C H
CH
Biodynamics Institute, Louisiana State University, Baton Rouge 70803, USA. chkenne@lsu.edu
Cueto
R
R
Belinsky
S A
SA
Lechner
J F
JF
Pryor
W A
WA
D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism; D008565:Membrane Proteins; D009363:Neoplasm Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D012333:RNA, Messenger / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014407:Tumor Cells, Cultured
D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D005656:Fungal Proteins; C118806:MTH1 protein, S cerevisiae; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins