The natriuretic peptide (ovCNP-39) from platypus (Ornithorhynchus anatinus) venom relaxes the isolated rat uterus and promotes oedema and mast cell histamine release.
In this study we characterise the ability of a C-type natriuretic peptide from platypus (Ornithorhynchus anatinus) venom (ovCNP-39) to relax the rat uterus in vitro and we investigate the possibility that ovCNP-39 contributes to the acute effects of envenomation, which include oedema, pain and erythema. We have found that both ovCNP-39 and the endogenous C-type natriuretic peptide, CNP-22, produce oedema in the rat paw and release histamine from rat peritoneal mast cells. Two synthetic peptides, ovCNP-39(1-17) and ovCNP-39(18-39), corresponding to the N- and C-termini, respectively, are equipotent histamine releasers, suggesting that ovCNP-39 and other natriuretic peptides do not act through conventional natriuretic peptide receptors on mast cells.
de Plater
G M
GM
The John Curtin School of Medical Research, Australian National University, Canberra ACT.
Martin
R L
RL
Milburn
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D004487:Edema / Q000139:chemically induced*; D005260:Female; D006636:Histamine Release / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D008969:Molecular Sequence Data; D009126:Muscle Relaxation; D020098:Natriuretic Peptide, C-Type; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D010987:Platypus; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D017386:Sequence Homology, Amino Acid; D014599:Uterus / Q000187:drug effects* / Q000502:physiology; D014688:Venoms / Q000737:chemistry / Q000633:toxicity*
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011506:Proteins; D014688:Venoms; C114236:ovCNP-39 peptide; D020098:Natriuretic Peptide, C-Type