title: Fucosyltransferase-catalyzed formation of L-galactosylated Lewis structures.
abstract: The Lewis (alpha 1-3/4) fucosyltransferase isolated from human milk could be used for preparative fucosylations of the disaccharide acceptors Gal(beta 1-3)GlcNAc(beta 1-O)R (at position OH-4) and Gal(beta 1-4)GlcNAc(beta 1-O)R (at position OH-3) [R = (CH2)8COOMe]. As donors GDP-L-Gal and deoxygenated derivatives were used to lead to a series of novel modified trisaccharides of the Lewis(a) and the Lewis(x) type, respectively.
authors.affiliation: Department of Chemistry, University of Alberta, Edmonton, Canada.
authors.forename: K
authors.initials: K
authors.lastname: Stangier
authors.forename: M M
authors.initials: MM
authors.lastname: Palcic
authors.forename: D R
authors.initials: DR
authors.lastname: Bundle
authors.forename: O
authors.initials: O
authors.lastname: Hindsgaul
authors.forename: J
authors.initials: J
authors.lastname: Thiem
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D005697:Galactosides / Q000138:chemical synthesis*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000201:enzymology; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis*
chemical_list: D004187:Disaccharides; D005697:Galactosides; D007983:Lewis Blood Group Antigens; D014312:Trisaccharides; D006154:Guanosine Diphosphate Fucose; D005647:Fucosyltransferases