Cloning of the histidine biosynthetic genes of Corynebacterium glutamicum: organization and sequencing analysis of the hisA, impA, and hisF gene cluster.
The hisA and hisF genes of Corynebacterium glutamicum were cloned by transforming histidine auxotrophic Escherichia coli with the genomic DNA library. They are two of the eight genes that participate in the histidine biosynthetic pathway. Cloned DNA fragments containing the genes can also complement hisH and hisI auxotrophs of Escherichia coli, suggesting that the four genes are clustered in the genome. We determined the nucleotide sequences of the minimal fragment containing the hisA and hisF genes, which are separated by the impA gene. The coding regions of the hisA and hisF genes are 245 and 257 amino acids in length with a predicted size of about 26 and 27 kDa, respectively. These are in good agreement with the sizes of proteins expressed in E. coli. A high similarity was observed in comparison of nucleotide sequences of each protein between C. glutamicum and other species, as well as those between hisA and hisF genes of C. glutamicum.
Jung
S I
SI
Department of Biological Science, Sookmyung Women's University, Seoul, Korea.
Han
M S
MS
Kwon
J H
JH
Cheon
C I
CI
Min
K H
KH
Lee
M S
MS
D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D000595:Amino Acid Sequence; D000619:Aminohydrolases / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D003352:Corynebacterium / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics; D006639:Histidine / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases; D014162:Transfection / Q000235:genetics
D001426:Bacterial Proteins; D006639:Histidine; D012697:Serine Endopeptidases; C065791:impA protein, Salmonella typhimurium; C087227:imidazole glycerol phosphate synthase; D000619:Aminohydrolases; D019747:Aldose-Ketose Isomerases; C079656:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase