[Cloning and identification of fliCi gene of Salmonella typhimurium].
Purified Salmonella typhimurium 8705 chromosomal DNA was digested with EcoRI. DNA fragments above 400 bp were obtained by Sepharcyl S-400 chromatography and cloned into vector pGEM-3Zf(-), then transformed into host cell LC2a (hag- is recA-). One out of 6013 transformants was found to be motile and this clone was named pGI4015. Miniprep proved that pGI4015 contained an inserted fragment about 15.3 kb. Motility/inhibition tests as well as Southern blot hybridization showed that pGI4015 bear the H-1i gene of S. typhimurium. With the aid of BamHI and SalI site, sequences unrelated to flagellin in the cloned DNA fragment was removed, and a 3.8kb fragment containing fliC gene was subcloned.
Cai
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X
Department of Veterinary Medicine, Agricultural College, Yangzhou University.
Jiao
X
X
Zhang
R
R
Liu
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X
D003001:Cloning, Molecular; D005408:Flagellin / Q000235:genetics*; D005798:Genes, Bacterial; D012486:Salmonella typhimurium / Q000235:genetics*
D005408:Flagellin