Oxidative stress in patients with hepatitis, cirrhosis, and hepatoma evaluated by plasma antioxidants.
We have applied our method for the simultaneous detection of plasma ubiquinol-10 (reduced form) and ubiquinone-10 (oxidized form) (S. Yamashita and Y. Yamamoto, Anal. Biochem. 250, 66-73, 1997) to plasmas of normal subjects (n = 16) and patients with chronic active hepatitis (n = 28), liver cirrhosis (n = 16), and hepatocellular carcinoma (n = 20) to evaluate the pressure of oxidative stress in these patients. The average ubiquinone-10 percentages (+/- S.D.) in total ubiquinone-10 and ubiquinol-10 in the four groups were 6.4 +/- 3.3, 12.9 +/- 10.3, 10.6 +/- 6.8, and 18.9 +/- 11.1, respectively, indicating a significant increase in ubiquinone-10 percentage in patient groups in comparison to normal subjects. These results and a significant decrease in the plasma ascorbate level in patient groups indicate that oxidative stress is evident after the onset of hepatitis and the subsequent cirrhosis and liver cancer.
Yamamoto
Y
Y
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
Yamashita
S
S
Fujisawa
A
A
Kokura
S
S
Yoshikawa
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000097:blood; D015415:Biomarkers / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood; D002338:Carotenoids / Q000097:blood; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D005260:Female; D006521:Hepatitis, Chronic / Q000097:blood; D006801:Humans; D008103:Liver Cirrhosis / Q000097:blood; D008107:Liver Diseases / Q000097:blood*; D008113:Liver Neoplasms / Q000097:blood; D000077276:Lycopene; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000097:blood; D014810:Vitamin E / Q000097:blood; D019207:beta Carotene / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D002788:Cholesterol Esters; D019207:beta Carotene; D014451:Ubiquinone; D014810:Vitamin E; D002338:Carotenoids; C026663:ubiquinol-10; D002784:Cholesterol; C025204:Ubiquinone Q2; D001205:Ascorbic Acid; D000077276:Lycopene