title: The HIS4 gene from the yeast Kluyveromyces lactis.
abstract: The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb.
authors.affiliation: Departamento de Biologia Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Freire-Picos
authors.forename: M
authors.initials: M
authors.lastname: Hampsey
authors.forename: M E
authors.initials: ME
authors.lastname: Cerdán
mesh_terms: D000429:Alcohol Oxidoreductases; D000595:Amino Acid Sequence; D000619:Aminohydrolases; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D007716:Kluyveromyces / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D011755:Pyrophosphatases; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013329:Structure-Activity Relationship; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C092116:HIS4 protein, S cerevisiae; D000619:Aminohydrolases; D011755:Pyrophosphatases