[Kinetics of in vitro clonal growth of the murine MB-49 bladder tumor cell line].
OBJECTIVE
To analyze the in vitro growth kinetics of the MB-49 murine bladder tumor cell line.


METHODS
Cell proliferation and duplication time (DT) were determined by 595 nm absorbance analysis and hemocytometry with and without fetal calf serum (FCS).


RESULTS
The 595 nm absorbance and the hemocytometer cell count correlated according to the linear equation of cell number = 595 nm absorbance x 334.110-8.795 (r = 0.98). Cell proliferation was significantly higher in cultures with FCS (p < 0.001). However, a significant increase in the cell count (p < 0.01) was observed after 24 hours in cultures with and without FCS. DT was 21 and 50 hours for cultures with and without FCS, respectively.


CONCLUSIONS
This experiment confirms the nature of the tumor cells and permits establishing the precise growth conditions of the MB-49 cells in this laboratory.
Páez Borda
A
A
Unidad de Investigación Urológica, Hospital Universitario de Getafe, Madrid, España.
Angulo Frutos
J
J
Luján Galán
M
M
Llanes González
L
L
Berenguer Sánchez
A
A
D000818:Animals; D002455:Cell Division; D007700:Kinetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000473:pathology*
Cinética de crecimiento clonal in vitro de la línea de tumor vesical murino MB-49.