Magnetic resonance microimaging for noninvasive quantification of myocardial function and mass in the mouse.
The purpose of this work was to develop high-resolution cardiac magnetic resonance imaging techniques for the in vivo mouse model for quantification of myocardial function and mass. Eight male mice were investigated on a 7-Tesla MRI scanner. High-quality images in multiple short axis slices (in-plane resolution 117 microm2, slice thickness 1 mm) were acquired with an ECG-gated cine sequence. Left ventricular end-diastolic and end-systolic volumes and mass were calculated from segmented slice volumes. There was precise agreement of left ventricular mass determined ex vivo and by MRI. Intraobserver (5%) and interobserver (5%) variability of in vivo MR measurements were low.
Ruff
J
J
Physikalisches Institut (EP5), Universität Würzburg, Germany.
Wiesmann
F
F
Hiller
K H
KH
Voll
S
S
von Kienlin
M
M
Bauer
W R
WR
Rommel
E
E
Neubauer
S
S
Haase
A
A
D000818:Animals; D003971:Diastole / Q000502:physiology; D006352:Heart Ventricles / Q000033:anatomy & histology*; D007089:Image Enhancement / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000295:instrumentation / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008953:Models, Anatomic; D015588:Observer Variation; D019047:Phantoms, Imaging; D012016:Reference Values; D012680:Sensitivity and Specificity; D013599:Systole / Q000502:physiology; D016277:Ventricular Function, Left / Q000502:physiology*; D016278:Ventricular Function, Right / Q000502:physiology*