Differential binding of human blood group Sd(a+) and Sd(a-) Tamm-Horsfall glycoproteins with Dolichos biflorus and Vicia villosa-B4 agglutinins.
The binding patterns of human blood group Sd(a+) and Sd(a-) Tamm-Horsfall glycoproteins (THGPs) with respect to four GalNAc specific agglutinins were studied by quantitative precipitin assay (QPA) and enzyme linked lectinosorbent assay (ELLSA). Of the native and asialo Sd(a+) and Sd(a-) THGP tested by QPA and ELLSA, only native and asialo Sd(a+) bound well with Dolichos biflorus (DBA) and Vicia villosa-B4 (VVA-B4), while Sd(a-) THGP reacted poorly with these two lectins. Neither Sd(a+) nor Sd(a-) THGPs reacted with two other GalNAc alpha-anomer specific lectins: Codium fragile subspecies tomentosoides and Artocarpus integrifolia. Furthermore, the binding of asialo Sd(a+)THGP-VVA-B4 and native Sd(a+)THGP-DBA through GalNAc beta--> was confirmed by inhibition assay. These results demonstrate that DBA and VVA-B4 are useful reagents to differentiate between Sd(a+) and Sd(a-) THGP.
Wu
A M
AM
Glycoimmunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan.
Wu
J H
JH
Watkins
W M
WM
Chen
C P
CP
Song
S C
SC
Chen
Y Y
YY
D000116:Acetylgalactosamine / Q000378:metabolism; D001789:Blood Group Antigens; D002240:Carbohydrate Sequence; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D037121:Plant Lectins; D011485:Protein Binding; D058949:Uromodulin
D001789:Blood Group Antigens; D037102:Lectins; D009088:Mucoproteins; D009844:Oligosaccharides; D037121:Plant Lectins; C548612:UMOD protein, human; D058949:Uromodulin; C042242:Vicia lectins; C036554:dolichos biflorus agglutinin; D000116:Acetylgalactosamine