Capillary electrophoresis in biochemical and clinical laboratoriesp selected attractive examples.
As demonstrated by selected examples from our laboratories, CE is a unique methodology for purity control of synthetic as well as natural tissue-isolated biopolymers, a prerequisite before reliable biotestings should be performed. A combination of rapid matrix-assisted laser desorption ionization mass and CE electrophoretic mobility determinations facilitates primary sequence determinations of enzymatic peptide digest mixtures often making costly Edman degradations unnecessary. The enormous separation efficiency and large variety of different possible separation modes in CE, allow detection of single components in complex mixtures which is demonstrated by the apolipoprotein A-I determination in human blood serum in this communication.
Voelter
W
W
Abteilung für Physikalische Biochemie, Universität Tübingen, Germany.
Schütz
J
J
Tsitsiloni
O E
OE
Weiler
A
A
Grübler
G
G
Paulus
G
G
Stoeva
S
S
Lehmann
R
R
D000465:Algorithms; D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000032:analysis; D001671:Biochemistry / Q000295:instrumentation*; D001704:Biopolymers / Q000032:analysis*; D001774:Blood Chemical Analysis; D019075:Electrophoresis, Capillary / Q000706:statistics & numerical data*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007753:Laboratories; D007755:Laboratories, Hospital; D008969:Molecular Sequence Data; D009391:Nephelometry and Turbidimetry; D010446:Peptide Fragments / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013947:Thymosin / Q000032:analysis
D001053:Apolipoproteins; D001704:Biopolymers; D010446:Peptide Fragments; D013947:Thymosin