Human in vitro cell lines verification by minisatellite DNA restriction fragment length polymorphism.
Four families of human in vitro cell lines were tested for minisatellite restriction fragment length polymorphism (RFLP) using multilocus probes MZ1.3 and/or 33.15 after digestion of DNA with restriction enzymes HinfI or HaeIII. These results confirmed that (i) the RFLP pattern is relatively stable in established cell lines and, therefore, could be used as a specific marker of a cell line identity, (ii) the use of MZ1.3 and 33.15 probes permits the identification of hybridomas and (iii) one of the cell lines tested, a lymphoblastoid cell line HAJ, may possess a hot spot of mutation.
Mańczak
M
M
Laboratory of Immunogenetics, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Zielińska-Dawidziak
M
M
Prussak
M
M
Dubis
J
J
Kość
A
A
Duś
D
D
Wojdat
E
E
Kuśnierczyk
P
P
D002460:Cell Line; D003110:Colonic Neoplasms / Q000235:genetics; D019073:HT29 Cells; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics; D018895:Microsatellite Repeats; D009154:Mutation; D012150:Polymorphism, Restriction Fragment Length; D014407:Tumor Cells, Cultured