Mutants of Citrobacter freundii that transport and utilize melibiose.
We have isolated mutants of Citrobacter freundii that can grow on melibiose. Inducible alpha-galactosidase activity and melibiose transport activity were detected in the mutant cells but not in the wild-type cells. We detected a DNA region which hybridized with melB (the gene for the melibiose transporter) DNA of Escherichia coli in the chromosomal DNA of wild-type C. freundii. Protons, but not sodium ions, were found to be the coupling cations for melibiose (and methyl-beta-D-thiogalactoside) transport in the mutant cells.
Okazaki
N
N
Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Xu
X J
XJ
Shimamoto
T
T
Kuroda
M
M
Wilson
T H
TH
Tsuchiya
T
T
D001692:Biological Transport; D016971:Citrobacter freundii / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D007700:Kinetics; D008553:Melibiose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D027981:Symporters; D000519:alpha-Galactosidase / Q000378:metabolism*
D026901:Membrane Transport Proteins; D027981:Symporters; C021548:melibiose permease; D008553:Melibiose; D000519:alpha-Galactosidase