Identification of multispecific organic anion transporter 2 expressed predominantly in the liver.
In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE2, dicarboxylates and p-aminohippurate. [14C]Salicylate uptake via NLT was saturable (Km = 88.8 +/- 23.4 microM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver >> kidney). We propose that NLT be renamed OAT2.
Sekine
T
T
Kyorin University School of Medicine, Department of Pharmacology and Toxicology, Mitaka, Tokyo, Japan.
Cha
S H
SH
Tsuda
M
M
Apiwattanakul
N
N
Nakajima
N
N
Kanai
Y
Y
Endou
H
H
D000818:Animals; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017136:Ion Transport; D008099:Liver / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D029362:Organic Anion Transporters, Sodium-Independent; D051381:Rats; D012459:Salicylates / Q000378:metabolism; D020156:Salicylic Acid; D013379:Substrate Specificity; D014982:Xenopus laevis
D002352:Carrier Proteins; D008565:Membrane Proteins; D029362:Organic Anion Transporters, Sodium-Independent; D012459:Salicylates; C088800:Slc22a7 protein, rat; D020156:Salicylic Acid