title: [Studies on late replication bands of giant panda (Ailuropoda melanoleuca) chromosomes].
abstract: Using the cultured giant panda (Ailuropoda melanoleuca) lymphocytes as experimental material, we carried out the terminal marking on the chromosomes which were in replication by adding BrdU (a final concentration: 10 micrograms/ml) about four hours before harvesting the cells. The chromosomes marked by BrdU were proceeded by staining with acridine orange solution (0.05%), irradiated by ultraviolet and counter-stained by Giemsa, we obtained clear chromosomes replication patterns. According to the different replication bands, every chromosome's characteristics in late replication behavior could be identified. In the two X chromosomes of female individual, one X chromosome is obviously much later than the other one. Especially in the large area near centromere on the long arm of late replicating X chromosome. In the male individual, there is also a large area on the long arm of chromosome Y which replicates very late, but the end of long arm of chromosome Y replicates much earlier.
authors.affiliation: Biology Department, Sichuan University, Chengdu.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Wang
authors.forename: X Z
authors.initials: XZ
authors.lastname: Wang
authors.forename: Y H
authors.initials: YH
authors.lastname: Yang
authors.forename: Y S
authors.initials: YS
authors.lastname: Qian
authors.forename: W Y
authors.initials: WY
authors.lastname: Chen
authors.forename: Z S
authors.initials: ZS
authors.lastname: Wang
authors.forename: L S
authors.initials: LS
authors.lastname: Fei
authors.forename: H W
authors.initials: HW
authors.lastname: Chen
authors.forename: G X
authors.initials: GX
authors.lastname: HE
authors.forename: Y F
authors.initials: YF
authors.lastname: Song
mesh_terms: D000818:Animals; D002871:Chromosome Banding; D004261:DNA Replication; D005260:Female; D008297:Male; D001503:Ursidae / Q000235:genetics*