Effects of gosha-jinki-gan, a kampo medicine, on peripheral tissue blood flow in streptozotocin-induced diabetic rats.
We examined the effects of Gosha-jinki-gan on peripheral tissue blood flow in streptozotocin-induced diabetic rats and its mechanism. The decrease in peroneal muscular blood flow in diabetic rats was dose-dependently improved by treatment with Gosha-jinki-gan (0.1-1 g/kg/day, p.o.) for 4 weeks. Investigation of the time course of blood flow in the hind paws revealed that single-dose of Gosha-jinki-gan (0.3, 1.5 g/kg, i.d.) increased peripheral blood flow. Peripheral blood flow-increasing effects of Gosha-jinki-gan (1.5 g/kg, i.d.) were reduced in combination with atropine (1 mg/kg, i.v.), and disappeared by pretreatment with NG-nitro-L-arginine methyl ester (10 mg/kg, i.v.). Levels of aortic guanosine 3',5'-cyclic monophosphate were dose-dependently increased by administration of Gosha-jinki-gan (0.3, 1.5 g/kg, p.o). These results suggest that Gosha-jinki-gan has vasodilating effects via increases in nitric oxide production. Gosha-jinki-gan may be useful for treating peripheral circulation disorders in the diabetic state.
Suzuki
Y
Y
Department of Pathophysiology & Therapeutics, Hoshi University, Tokyo, Japan.
Goto
K
K
Ishige
A
A
Komatsu
Y
Y
Kamei
J
J
D000818:Animals; D001011:Aorta / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006614:Hindlimb / Q000098:blood supply*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects; D014665:Vasodilator Agents / Q000494:pharmacology*
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D004791:Enzyme Inhibitors; D014665:Vasodilator Agents; C076165:gosha-jinki-gan-ryo extract; D000242:Cyclic AMP; D006152:Cyclic GMP