Immunorheophoresis for the diagnosis of infectious bursal disease.
The immunorheophoresis (IR) technique was used for the detection of infectious bursal disease antigen from bursae collected from field cases and experimentally infected chickens. When these results were compared with that of the agar gel immunodiffusion (AGID) test, they showed excellent agreement as determined by kappa value. However, the time taken for the appearance of the precipitin lines was reduced from 14-24 hr in the AGID test to 3-5 hr in the IR technique.
Raj
G D
GD
Vaccine Research Centre, Tamilnadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Madras, India.
Jayakumar
V
V
Thangavelu
A
A
Koteeswaran
A
A
Venugopalan
A T
AT
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D002060:Bursa of Fabricius / Q000821:virology; D002645:Chickens; D005779:Immunodiffusion / Q000662:veterinary; D007122:Immunoelectrophoresis / Q000379:methods / Q000662:veterinary*; D007243:Infectious bursal disease virus / Q000276:immunology* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000175:diagnosis*; D012680:Sensitivity and Specificity
D000956:Antigens, Viral