Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice.
There is increasing evidence showing that cereal alpha-amylase gene expression is controlled not only by the classical hormonal regulation, but also by feed-back sugar repression. We demonstrated by in situ hybridization that the sugar repression of rice alpha-amylase gene RAmy3D takes place in scutellar epithelium cells of callus-forming rice embryos. We also used a transient expression system to study the cis-acting elements involved in the sugar repression of the RAmy3D promoter activity. Site-directed mutagenesis of the 50-bp nucleotide sequence from -172 to -123 revealed that consensus sequences of G motif (TACGTA) and TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for sugar repression. The promoter sequences required for sugar repression are reported and discussed.
Toyofuku
K
K
Bioscience Center and Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Umemura
T
T
Yamaguchi
J
J
D001483:Base Sequence; D016384:Consensus Sequence; D004794:Enzyme Repression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D006147:Guanine; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology* / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D000516:alpha-Amylases / Q000096:biosynthesis* / Q000235:genetics*
D006147:Guanine; D000516:alpha-Amylases