title: Enhanced broth media for selective growth of Vibrio vulnificus.
abstract: Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.
authors.affiliation: Department of Food Science and Human Nutrition, University of Florida, Gainesville 32611, USA.
authors.forename: W Y
authors.initials: WY
authors.lastname: Hsu
authors.forename: C I
authors.initials: CI
authors.lastname: Wei
authors.forename: M L
authors.initials: ML
authors.lastname: Tamplin
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010461:Peptones / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D010461:Peptones; D012965:Sodium Chloride