title: Novel testis-specific protein that interacts with heat shock factor 2.
abstract: Although heat shock factor 2 (HSF2) binds to heat shock element (HSE) constitutively during differentiation, development and spermatogenesis, little is known about the nature and mechanism of transcriptional control of heat shock genes by HSF2. We screened a human testis cDNA library for proteins that can associate with HSF2 by the yeast two-hybrid system, and isolated clones encoding a novel protein, designated HSF2 binding protein (HSF2BP), that associates with HSF2 in vitro and in vivo and is specifically expressed in testis. The interaction seemed to occur between the trimerization domain of HSF2 and the amino terminal hydrophilic region of HSF2BP that comprises two leucine zipper motifs. HSF2BP may therefore be involved in modulating HSF2 activation in testis.
authors.affiliation: HSP Research Institute, Kyoto Research Park, Kyoto 600, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoshima
authors.forename: T
authors.initials: T
authors.lastname: Yura
authors.forename: H
authors.initials: H
authors.lastname: Yanagi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D015723:Gene Library; D006360:Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D016350:Leucine Zippers / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
chemical_list: D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D011506:Proteins; D014157:Transcription Factors; C070389:HSF2 protein, human