title: Cloning and characterization of human estrogen receptor beta isoforms.
abstract: Multiple transcripts which arise from the human estrogen receptor beta (ER beta) gene have been characterized. Three full length isoforms of the hER beta gene, designated hER beta 1-3, were identified in a testis cDNA library. An additional two isoforms, designated hER beta 4 and hER beta 5, were identified by PCR amplification from testis cDNA and from the MDA-MB 435 cell line. hER beta 1 corresponds to the previously described hER beta. All five isoforms diverge at a common position within the predicted helix 10 of the ligand binding domain of hER beta, with nucleotide sequences consistent with differential exon usage. The hER beta isoform mRNAs displayed a differential pattern of expression in human tissues and in tumor cell lines when analyzed by RT-PCR. Further characterization of the three full length isoforms, hER beta 1-3, by in vitro band shift studies indicated that the isoforms were able to form DNA-binding homodimers and heterodimers with each other and with the ER alpha subtype.
authors.affiliation: Department of Molecular Sciences, Glaxo Wellcome Research and Development, Research Triangle Park, North Carolina 27709, USA. jtm36008@glaxowellcome.com
authors.forename: J T
authors.initials: JT
authors.lastname: Moore
authors.forename: D D
authors.initials: DD
authors.lastname: McKee
authors.forename: K
authors.initials: K
authors.lastname: Slentz-Kesler
authors.forename: L B
authors.initials: LB
authors.lastname: Moore
authors.forename: S A
authors.initials: SA
authors.lastname: Jones
authors.forename: E L
authors.initials: EL
authors.lastname: Horne
authors.forename: J L
authors.initials: JL
authors.lastname: Su
authors.forename: S A
authors.initials: SA
authors.lastname: Kliewer
authors.forename: J M
authors.initials: JM
authors.lastname: Lehmann
authors.forename: T M
authors.initials: TM
authors.lastname: Willson
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D047629:Estrogen Receptor beta; D004967:Estrogens / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D007536:Isomerism; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D013737:Testis
chemical_list: D004268:DNA-Binding Proteins; D047629:Estrogen Receptor beta; D004967:Estrogens; D012333:RNA, Messenger; D011960:Receptors, Estrogen