Recovering and reamplifying of the differentially expressed cDNA bands isolated from mRNA differential display. A modified method.
Methods for retrieving and reamplifying the differentially expressed cDNA bands have been modified. Direct reamplification of differentially expressed bands after cutting from a polyacrylamide gel (PAG) followed by a simple rinse and crush step has proved to be more convenient and effective than the traditional glycogen-precipitation method. Combination of 30 cycles of differential display (DD) polymerase chain reaction (PCR) and 20 cycles of standard PCR reaction also yielded higher reamplification rates.
Wang
H
H
Department of Tumor Molecular Biology, Institute of Basic Medical Sciences, Beijing, P.R. China.
Xia
Z
Z
Wang
Y
Y
Li
C
C
Sun
L Y
LY
D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger