Spinach cytosolic fructose-1,6-bisphosphatase: II. Light effect on its expression.
The effect of light on the expression of spinach (Spinacia oleracea) cytosolic fructose-1,6-bisphosphatase (FBPase) was determined by the level of mRNA, protein content, and enzyme activity. It was found that its expression and activity were constant and stable during normal daily conditions as well as under continuous light or dark conditions. However, two different mRNAs were detected; one transcript was expressed all the time, while the other was detected only during prolonged dark periods. Analysis of the expression of the mRNAs at the protein level using an activity gel showed that this "darkness-specific" mRNA encoded a separate, distinct polypeptide. Thus, our data suggest that cytosolic FBPase is encoded by a small multigene family.
Hur
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Department of Biology, Chungnam National University, Taejeon, Korea. ykhur@hanbat.chungnam.ac.kr
Vasconcelos
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D015139:Blotting, Southern; D002940:Circadian Rhythm / Q000235:genetics; D003600:Cytosol / Q000201:enzymology / Q000528:radiation effects; D003624:Darkness; D006597:Fructose-Bisphosphatase / Q000235:genetics / Q000528:radiation effects; D015870:Gene Expression / Q000235:genetics / Q000528:radiation effects; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000528:radiation effects; D017343:Genes, Plant / Q000235:genetics / Q000528:radiation effects; D008027:Light; D017440:Photoperiod; D012333:RNA, Messenger / Q000378:metabolism / Q000528:radiation effects; D018724:Spinacia oleracea / Q000201:enzymology* / Q000528:radiation effects / Q000648:ultrastructure*; D013997:Time Factors
D012333:RNA, Messenger; D006597:Fructose-Bisphosphatase