title: [Immunogenicity of recombinant S. typhimurium ex. pressing a hybrid antigen of Plasmodium falciparum].
abstract: We have expressed a 74-peptide hybrid Plasmodium falciparum antigen as fusion protein in attenuated Salmonella typhimurium SL3261. Live organisms were orally immunized Rabbits with a dose of 2 x 10(9)cfu. Specific anti-serum were detected by ELISA after immunization. Obvious Delayed type hypersensitivity (DTH) could be induced by PfAg and GZ-C antigen. The recombinant vaccine had no evident side-effects to the hosts. Our studies indicate that attenuated Salmonella typhimurium SL3261 can express synthetic P. falciparum antigen with several epitopes and live organisms can activate special cell-mediated immunity and humoral immunity.
authors.affiliation: Institute of Molecular Biology, First Military Medical University, Guangzhou.
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Ren
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.forename: X
authors.initials: X
authors.lastname: Zhong
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000953:Antigens, Protozoan / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology; D008297:Male; D010963:Plasmodium falciparum / Q000276:immunology*; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology*; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D011993:Recombinant Fusion Proteins; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic