[Detection of exotoxins of the pathogens of anaerobic gas infection using enzyme immunoassay].
To detect the exotoxins of the causative agents of the main anaerobic gas infection (Clostridium perfringens, C.oedematiens, C.histolyticum, C.septicum) a rapid and easily reproducible variant of the enzyme-linked immunosorbent assay (ELISA), based on the use of the sandwich modification activated due to avidin-biotin interaction, was proposed. The possibility of using the avidin-biotin variant of ELISA for the detection of C.perfringens, C.oedematiens, C.histolyticum and C.septicum toxins in experimental gas gangrene in guinea pigs was shown. The method made it possible to reproducibly detect 0.02-0.2 ng of antigens (gangrene toxins-toxoids) with the immunoassay being highly specific.
Griaznova
D V
DV
Nikolaeva
A M
AM
Efimova
N P
NP
D000693:Anaerobiosis; D003013:Clostridium; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000032:analysis*; D005738:Gas Gangrene / Q000382:microbiology / Q000517:prevention & control*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D005098:Exotoxins
Vyiavlenie ékzotoksinov osnovnykh vozbuditeleĭ anaérobnoĭ gazovoĭ infektsii s pomoshch'iu immunofermentnogo analiza.