title: Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.
abstract: Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.
authors.affiliation: School of Biology, Georgia Institute of Technology, Atlanta 30332, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Wartell
authors.forename: S
authors.initials: S
authors.lastname: Hosseini
authors.forename: S
authors.initials: S
authors.lastname: Powell
authors.forename: J
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009691:Nucleic Acid Denaturation; D017354:Point Mutation; D011110:Polymorphism, Genetic; D013696:Temperature; D013816:Thermodynamics
chemical_list: D004247:DNA