Taurine-stimulated outgrowth from the retina is impaired by protein kinase C activators and phosphatase inhibitors.
Taurine increases neurite elongation of post-crush goldfish retinal explants, as well as the number of outgrowing isolated cells from goldfish and rat retina in culture. The trophic effect of taurine is related to an elevation in calcium flux rather than increased cell proliferation. Since taurine regulates phosphorylation in rat retina, we investigated if this process could be involved in the mechanism of taurine action on outgrowth. Control and taurine-supplemented post-crush goldfish retinal explants were cultured in the presence of protein kinase C activators or phosphatase inhibitors, and the length of neurites was measured after five days in culture. In some cases, there was an inhibition of the stimulatory effect of taurine without a modification in basal outgrowth. In others, outgrowth of control explants was also reduced. A certain level of protein phosphorylation seems to be critical for the trophic effect of taurine in the retina.
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Laboratorio de Neuroquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Cubillos
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D000818:Animals; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006054:Goldfish; D066298:In Vitro Techniques; D016501:Neurites / Q000187:drug effects / Q000502:physiology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D011493:Protein Kinase C / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D013654:Taurine / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D013654:Taurine; D011493:Protein Kinase C; D010749:Phosphoprotein Phosphatases