title: cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme.
abstract: A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.
authors.affiliation: Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yanagisawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: M
authors.initials: M
authors.lastname: Suiko
authors.forename: Y
authors.initials: Y
authors.lastname: Takami
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Takayanagi
authors.forename: Y
authors.initials: Y
authors.lastname: Natori
authors.forename: M C
authors.initials: MC
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents / Q000378:metabolism; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013430:Sulfate Adenylyltransferase / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D003432:Cross-Linking Reagents; D017931:DNA Primers; D017853:Phosphotransferases (Alcohol Group Acceptor); C019617:adenylylsulfate kinase; D013430:Sulfate Adenylyltransferase