[Storage of human bone marrow before transplantation at 4 degrees C. The effect of cell proliferation potential particularly in hematopoietic cloned cell lines].
The possibility of storage of human bone marrow CD34+ cells at 4 degrees C for bone marrow transplantation purposes was investigated. The cells were placed in Iscove medium supplemented with 20% serum (15% bovine calf serum + 5% human AB serum) for three weeks at 4 degrees C. During storage time clonogenicity of granulocytic-monocytic (CFU-GM) erythropoietic (BFU-E) and megakaryocytic (CFU-Meg) progenitors were investigated. It turns out that it is possible to keep human CD34+ cells at 4 degrees C for at least few days before transplantation. At day four of storage the number of CFU-GM and BFU-E cells still exceeded 50% of cells present at day 0. We have found however, CFU-Meg progenitors in comparison to others are much more sensitive to metabolic storage stress. This enhanced sensitivity of megakaryocytic progenitors could explain at least partially the well known ++phenomenon of retardation of thrombopoietic recovery in patients undergoing bone marrow transplantation.
Ratajczak
M Z
MZ
Instytutu-Centrum Medycyny Doświadczalnej i Klinicznej PAN.
Skorski
T
T
Kuczyński
W I
WI
Ratajczak
J
J
D018952:Antigens, CD34 / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology; D016026:Bone Marrow Transplantation; D002452:Cell Count; D002455:Cell Division; D002999:Clone Cells; D015925:Cryopreservation / Q000379:methods*; D006801:Humans; D013234:Stem Cells / Q000166:cytology
D018952:Antigens, CD34
Przechowywanie ludzkiego szpiku przed przeszczepieniem w temperaturze 4 degrees C. Wpływ na potencjał proliferacyjny komórek poszczególnych linii krwiotwórczych.