Growth inhibition by overexpression of human DEAD box protein rck/p54 in cells of a guinea pig cell line.
We transfected cells of a guinea pig cell line with RCK cDNA inserted in a pIRES1neo expression vector. The overexpression of rck/p54 was confirmed by Western blot and RT-PCR analysis. In two clones expressing rck/p54, the cell growth was highly inhibited; and their anchorage-independent growth, which is an important character of malignant transformation, was not found. These findings are the first evidence that the overexpression of a DEAD box protein/RNA helicase could inhibit substantially cell growth at the translational level.
Akao
Y
Y
Gifu International Institute of Biotechnology, Yagi Memorial Park, Mitake, Japan. yagiab@po.sphere.ne.jp
Mizoguchi
H
H
Ohishi
N
N
Yagi
K
K
D000818:Animals; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D053487:DEAD-box RNA Helicases; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000235:genetics; D006168:Guinea Pigs; D006801:Humans; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D012316:RNA Nucleotidyltransferases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014407:Tumor Cells, Cultured
D006131:Growth Inhibitors; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D012316:RNA Nucleotidyltransferases; C094683:DDX6 protein, human; D053487:DEAD-box RNA Helicases