title: Characterization and targeted disruption of a glycosyltransferase gene in the tylosin producer, Streptomyces fradiae.
abstract: An open reading frame, designated tylN, has been identified by sequence analysis at one end of the tylosin biosynthetic gene cluster of Streptomyces fradiae, alongside a cluster of genes encoding the biosynthesis of dTDP-deoxyallose. This 6-deoxyhexose sugar is converted to mycinose, via bis O-methylation, following attachment to the polyketide lactone during tylosin biosynthesis. The deduced product of tylN is similar to several glycosyltransferases, authentic and putative, and displays a consensus sequence motif that appears to be characteristic of a sub-group of such enzymes. Specific disruption of tylN within the S. fradiae genome resulted in the production of demycinosyl-tylosin, whereas other glycosyltransferase activities involved in tylosin biosynthesis were not affected. Evidently, tylN encodes deoxyallosyl transferase.
authors.affiliation: Department of Biochemistry, University of Leicester, Leicester, LE1 7RH, UK.
authors.forename: V T
authors.initials: VT
authors.lastname: Wilson
authors.forename: E
authors.initials: E
authors.lastname: Cundliffe
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D018390:Gene Targeting; D005798:Genes, Bacterial; D016695:Glycosyltransferases / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D015645:Tylosin / Q000031:analogs & derivatives / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D016695:Glycosyltransferases; D015645:Tylosin