Requirement of the CD8+ or CD4+ T lymphocyte subsets for the rejection of lymphoma and fibrosarcoma grafts studied in gene knockout hosts.
Rejections of the retrovirus induced lymphomas (ALC and RBL-5) and the methylcholanthrene (MCA) induced fibrosarcoma (MC57X) grafts were tested in syngeneic CD8 and CD4 single and double knockout C57BL/6 mice. The results with the lymphomas showed that the CD8+ T cell deficiency prevented the development of rejection response induced by immunization. Deficiency of the CD4+ T subset abrogated also the rejection of ALC. Immunity against the fibrosarcoma cells developed in both type of single knockout mice, but not in the ones which lacked both CD4+ and CD8+ T cells. Thus CD8+ T cells were required for rejection of the lymphoma cells, while the CD4+ T cells only mediated a weak response. In absence of CD8+ T cells, CD4+ T cells were sufficient to reject the fibrosarcoma cells.
Wen
T
T
Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, Canada.
Trümper
L
L
Fung-Leung
W
W
Rahemtulla
A
A
Klein
E
E
Klein
G
G
Mak
T W
TW
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002273:Carcinogens / Q000008:administration & dosage; D017690:Cell Transplantation; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology*; D006084:Graft Rejection / Q000276:immunology*; D016399:Lymphoma, T-Cell / Q000276:immunology*; D008297:Male; D008748:Methylcholanthrene / Q000008:administration & dosage; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011925:Rauscher Virus / Q000502:physiology
D002273:Carcinogens; D008748:Methylcholanthrene