title: Structure of the O-antigen of Vibrio cholerae O155 that shares a putative D-galactose 4,6-cyclophosphate-associated epitope with V. cholerae O139 Bengal.
abstract: The O-specific polysaccharide of Vibrio cholerae 0155 was studied by sugar and methylation analyses, dephosphorylation with 48% hydrofluoric acid, 1H- and 13C-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and heteronuclear single-quantum coherence (HSQC) experiments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: carbohydrate sequence [see text]. An unusual component, D-galactose 4,6-cyclophosphate, has been reported previously as a component of the capsular polysaccharide and O-antigen of V. cholerae O139 Bengal and appears to be responsible for the known serological cross-reactivity between V. cholerae O139 and O155.
authors.affiliation: Karolinska Institute, Clinical Research Center, Huddinge University Hospital, Sweden.
authors.forename: S N
authors.initials: SN
authors.lastname: Senchenkova
authors.forename: G V
authors.initials: GV
authors.lastname: Zatonsky
authors.forename: A S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Y A
authors.initials: YA
authors.lastname: Knirel
authors.forename: P E
authors.initials: PE
authors.lastname: Jansson
authors.forename: A
authors.initials: A
authors.lastname: Weintraub
authors.forename: M J
authors.initials: MJ
authors.lastname: Albert
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions / Q000276:immunology; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005695:Galactosephosphates / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D017421:Sequence Analysis; D012699:Serology; D014734:Vibrio cholerae / Q000276:immunology*
chemical_list: D000939:Epitopes; D005695:Galactosephosphates; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; C095960:galactose 4,6-cyclophosphate