Specific binding of tetratricopeptide repeat proteins to the C-terminal 12-kDa domain of hsp90.
The molecular chaperone hsp90 in the eukaryotic cytosol interacts with a variety of protein cofactors. Several of these cofactors have protein domains containing tetratricopeptide repeat (TPR) motifs, which mediate binding to hsp90. Using a yeast two-hybrid screen, the 12-kDa C-terminal domain of human hsp90alpha (C90) was found to mediate the interaction of hsp90 with TPR-containing sequences from the hsp90 cofactors FKBP51/54 and FKBP52. In addition, the mitochondrial outer membrane protein hTOM34p was identified as a TPR-containing putative partner protein of hsp90. In experiments with purified proteins, the TPR-containing cofactor p60 (Hop) was shown to form stable complexes with hsp90. A deletion mutant of hsp90 lacking the C90 domain was unable to bind p60, whereas deletion of the approximately 25-kDa N-terminal domain of hsp90 did not affect complex formation. Both p60 and FKBP52 bound specifically to the C90 domain fused to glutathione S-transferase and competed with each other for binding. In reticulocyte lysate, the C90 fusion protein recognized the TPR proteins p60, FKBP52, and Cyp40. Thus, our results identify the C90 domain as the specific binding site for a set of hsp90 cofactors having TPR domains.
Young
J C
JC
Department of Cellular Biochemistry, Max-Planck-Institut für Biochemie, Am Klopferspitz 18A, D-82152 Martinsried, Germany.
Obermann
W M
WM
Hartl
F U
FU
D000818:Animals; D001665:Binding Sites; D002352:Carrier Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D005656:Fungal Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D006360:Heat-Shock Proteins / Q000378:metabolism; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D029543:Nucleocytoplasmic Transport Proteins; D009842:Oligopeptides / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D022021:Tacrolimus Binding Proteins
D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C089362:Hrb1 protein, S cerevisiae; D029543:Nucleocytoplasmic Transport Proteins; D009842:Oligopeptides; D039102:Poly(A)-Binding Proteins; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D022021:Tacrolimus Binding Proteins