title: Differential characteristics of two new tumorigenic cell lines of human breast carcinoma origin.
abstract: Permanent human tumor cell lines are an important tool for the study of breast cancer. Two new breast cancer cell lines (BrCa-MZ-01 and BrCa-MZ-02) were isolated from a solid tumor and a pleural effusion, respectively. One cell line was established from a medullary carcinoma, the other from a ductal carcinoma. These cells exhibit ultrastructural and immunohistochemical features of epithelial cells of mammary origin. Intermediate filament and cytokeratin typing showed a clear predominance of the simple-epithelial cytokeratins CK 8, CK 18 and CK 19, although the expression was reduced in comparison to the hormone receptor-positive reference cell lines MCF-7 and ZR-75-1. Both cell lines produced slow-growing tumors after subcutaneous (s.c.) transplantation of 1 x 10(7) viable tumor cells into nude mice. The cell line BrCa-MZ-01 expresses the estrogen and progesterone receptor, whereas the cell line BrCa-MZ-02 remains negative. Both cell lines are positive for secretion of platelet-derived growth factor (PDGF) and transforming growth factor-beta (TGF-beta), whereas interleukin-6 (IL-6) is only secreted by the cell line BrCa-MZ-02.
authors.affiliation: Department of Obstetrics and Gynecology, University of Ulm, Germany.
authors.forename: V J
authors.initials: VJ
authors.lastname: Möbus
authors.forename: R
authors.initials: R
authors.lastname: Moll
authors.forename: C D
authors.initials: CD
authors.lastname: Gerharz
authors.forename: D G
authors.initials: DG
authors.lastname: Kieback
authors.forename: O
authors.initials: O
authors.lastname: Merk
authors.forename: I B
authors.initials: IB
authors.lastname: Runnebaum
authors.forename: S
authors.initials: S
authors.lastname: Linner
authors.forename: L
authors.initials: L
authors.lastname: Dreher
authors.forename: H J
authors.initials: HJ
authors.lastname: Grill
authors.forename: R
authors.initials: R
authors.lastname: Kreienberg
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001940:Breast / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D018276:Carcinoma, Medullary / Q000235:genetics / Q000473:pathology* / Q000648:ultrastructure; D002455:Cell Division; D002460:Cell Line; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis; D007381:Intermediate Filament Proteins / Q000032:analysis / Q000096:biosynthesis; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis; D051379:Mice; D008819:Mice, Nude; D010982:Platelet-Derived Growth Factor / Q000032:analysis / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000032:analysis; D011980:Receptors, Progesterone / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000096:biosynthesis; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
chemical_list: D015850:Interleukin-6; D007381:Intermediate Filament Proteins; D010982:Platelet-Derived Growth Factor; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D016212:Transforming Growth Factor beta; D007633:Keratins