title: Inhibition of plasma extravasation after burns by D-myo-inositol-1,2,6-trisphosphate using digital image colour analysis.
abstract: D-myo-inositol-1,2,6-triphosphate (1,2,6-IP3) has beneficial effects in experimental, progressive burn-induced ischaemia and oedema. A 1 cm2 full-thickness burn was made in the skin of 20 rats with a hot aluminium rod followed by infusion of 1,2,6-IP3 (60 mg.kg.-1 h-1) or isotonic saline (n = 10 in each group). One hour later Evans blue was injected intravenously. Colour photographs of the area of the burn were taken in a standard manner before the burn and at intervals for three hours afterwards. The photographs were analysed by digital image colour analysis using normalised red-green-blue values. The increase in normalised blue values between 60 and 180 minutes after the burn was significantly reduced in animals treated with 1,2,6-IP3 compared with control animals (p < 0.001). Spectrophotometric analysis of extravasated Evans blue in the skin 180 minutes after the burn showed that it had been significantly inhibited by treatment with 1,2,6-IP3 (p < 0.001). In conclusion, digital image analysis allowed repeated evaluation over time and confirmed previous data about the ability of 1,2,6-IP3 to inhibit extravasation of plasma after burns.
authors.affiliation: Department of Plastic Surgery, Sahlgrenska University Hospital, Göteborg, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Tarnow
authors.forename: A
authors.initials: A
authors.lastname: Jönsson
authors.forename: U
authors.initials: U
authors.lastname: Mattsson
authors.forename: G
authors.initials: G
authors.lastname: Rimbäck
authors.forename: J
authors.initials: J
authors.lastname: Cassuto
mesh_terms: D000818:Animals; D002056:Burns / Q000150:complications / Q000503:physiopathology*; D002199:Capillary Permeability / Q000187:drug effects*; D004487:Edema / Q000209:etiology / Q000503:physiopathology; D005070:Evans Blue; D005119:Extravasation of Diagnostic and Therapeutic Materials; D007091:Image Processing, Computer-Assisted; D007295:Inositol Phosphates / Q000494:pharmacology*; D008297:Male; D010781:Photography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013053:Spectrophotometry
chemical_list: D007295:Inositol Phosphates; D005070:Evans Blue; C059368:atrinositol