title: Alkaline phosphatase activity is expressed in murine splenic B-lymphocytes sensitized in vivo with Tetanus toxoid.
abstract: Alkaline phosphatase (APase) activity and proliferative response to Tetanus toxoid (TT) were measured in murine splenic lymphocytes immunized in vivo with TT. APase activity was enhanced in TT-stimulated B-lymphocytes concomitant with an increase in the proliferative response in a dose-dependent manner. Cytochemical staining for APase using beta-naphthyl phosphate also showed an increase in APase positive cells in TT-stimulated lymphocyte population. The results suggest that membrane APase expression is a physiological phenomenon occurring in antigen-stimulated B-lymphocytes.
authors.affiliation: Department of Biochemistry, School of Life Sciences, University of Hyderabad, India.
authors.forename: K
authors.initials: K
authors.lastname: Padmaja
authors.forename: M
authors.initials: M
authors.lastname: Ramanadham
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000201:enzymology*; D003208:Concanavalin A / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000201:enzymology; D013601:T-Lymphocytes / Q000187:drug effects; D013745:Tetanus Toxoid / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D008070:Lipopolysaccharides; D008934:Mitogens; D013745:Tetanus Toxoid; D003208:Concanavalin A; D000469:Alkaline Phosphatase