title: [Change in regulation of catabolite-dependent lux-operon, cloned in a recombinant plasmid, under the effect of environmental factors].
abstract: Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (AprLux+) in Escherichia coli z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent lux-operon. On the other hand, long-term laboratory cultivation of recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
authors.affiliation: Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia.
authors.forename: E E
authors.initials: EE
authors.lastname: Maksimova
authors.forename: L Iu
authors.initials: LIu
authors.lastname: Popova
authors.forename: V V
authors.initials: VV
authors.lastname: Shpagina
authors.forename: V A
authors.initials: VA
authors.lastname: Beliavskaia
authors.forename: N S
authors.initials: NS
authors.lastname: Pechurkin
mesh_terms: D003001:Cloning, Molecular; D003470:Culture Media; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008163:Luminescent Measurements; D009876:Operon; D010957:Plasmids; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D004274:DNA, Recombinant
vernacular_title: Izmenenie reguliatsii katabolitzavisimogo lux-operona, klonirovannogo v rekominantnoĭ plazmide, pod vozdeĭstviem faktorov vneshneĭ sredy.