title: The mouse pudgy mutation disrupts Delta homologue Dll3 and initiation of early somite boundaries.
abstract: Pudgy (pu) homozygous mice exhibit clear patterning defects at the earliest stages of somitogenesis, resulting in adult mice with severe vertebral and rib deformities. By positional cloning and complementation, we have determined that the pu phenotype is caused by a mutation in the delta-like 3 gene (Dll3), which is homologous to the Notch-ligand Delta in Drosophila. Histological and molecular marker analyses show that the pu mutation disrupts the proper formation of morphological borders in early somite formation and of rostral-caudal compartment boundaries within somites. Viability analysis also indicates an important role in early development. The results point to a key role for a Notch-signalling pathway in the initiation of patterning of vertebrate paraxial mesoderm.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA. kkusumi@nimr.mrc.ac.uk
authors.forename: K
authors.initials: K
authors.lastname: Kusumi
authors.forename: E S
authors.initials: ES
authors.lastname: Sun
authors.forename: A W
authors.initials: AW
authors.lastname: Kerrebrock
authors.forename: R T
authors.initials: RT
authors.lastname: Bronson
authors.forename: D C
authors.initials: DC
authors.lastname: Chi
authors.forename: M S
authors.initials: MS
authors.lastname: Bulotsky
authors.forename: J B
authors.initials: JB
authors.lastname: Spencer
authors.forename: B W
authors.initials: BW
authors.lastname: Birren
authors.forename: W N
authors.initials: WN
authors.lastname: Frankel
authors.forename: E S
authors.initials: ES
authors.lastname: Lander
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D016695:Glycosyltransferases; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011506:Proteins / Q000378:metabolism; D019170:Somites / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D011506:Proteins; C064045:delta protein; D016695:Glycosyltransferases; C498392:Lfng protein, mouse