title: Lesion-induced changes in the expression of ciliary neurotrophic factor and its receptor in rat optic nerve.
abstract: There is evidence that ciliary neurotrophic factor (CNTF) is involved in reactive changes following lesions of the nervous system. To investigate, whether differences in the regulation of CNTF and CNTF receptor alpha (CNTFRalpha) contribute to the differences in PNS and CNS responses to injury, we have studied their expression on the mRNA and protein level in the rat optic nerve following a crush lesion to compare them with the situation in peripheral nerve. Seven days after the lesion, CNTF mRNA and protein levels were markedly decreased at the lesion site, concommitant with the disappearance of GFAP- and CNTF-immunopositive astrocytes. CNTF levels in proximal and distal parts were less affected. This was in contrast to the situation in the PNS, where CNTF was downregulated at and distal to the lesion site. Different from other CNS regions, optic nerve astrocytes expressed CNTFRalpha mRNA under normal conditions. Following lesion, CNTFRalpha was reduced substantially only in distal and proximal parts of the optic nerve but continued to be expressed at high levels at the lesion site, suggesting that GFAP-negative, CNTF-responsive cells are present there. Our results suggest that differences in lesion-induced changes in the optic and sciatic nerve reflect differences in the response to injury of astrocytes and Schwann cells. In the light of the known actions of CNTF in inducing astrogliosis, the expression pattern observed in the optic nerve indicates that CNTF and CNTFRalpha are involved in glial scar formation in the lesion area.
authors.affiliation: Institute of Anatomy I, University of Freiburg, Germany. kirsch@ruf.uni-freiburg.de
authors.forename: M
authors.initials: M
authors.lastname: Kirsch
authors.forename: T
authors.initials: T
authors.lastname: Schneider
authors.forename: M Y
authors.initials: MY
authors.lastname: Lee
authors.forename: H D
authors.initials: HD
authors.lastname: Hofmann
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D020934:Ciliary Neurotrophic Factor; D005260:Female; D005911:Gliosis / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D009409:Nerve Crush; D009416:Nerve Regeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009900:Optic Nerve / Q000378:metabolism* / Q000473:pathology / Q000502:physiology; D020221:Optic Nerve Injuries; D009928:Organ Specificity; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis* / Q000235:genetics; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis* / Q000235:genetics; D012583:Schwann Cells / Q000378:metabolism; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D014855:Wallerian Degeneration
chemical_list: D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases