The effect of galE gene inactivation on lipopolysaccharide profile of Helicobacter pylori.
The galE gene product, UDP-galactose 4-epimerase, mediates the incorporation of galactose in extracellular polysaccharide materials such as the O-side chain of lipopolysaccharide (LPS). The O-side chain in H. pylori LPS has been shown to cross-react with Lewis x and/or y blood group antigens, suggesting its potential involvement in H. pylori-linked autoimmune disease. To study its role in H. pylori LPS biosynthesis, the galE gene was cloned, sequenced, and a galE-knockout H. pylori strain was constructed. The H. pylori galE gene encoded a protein of 344 amino acids with a molecular weight of 39K. The LPS profile from the galE-knockout H. pylori strain showed a lower molecular weight than that of the parental strain, indicating the involvement of the galE gene in LPS biosynthesis of H. pylori.
Kwon
D H
DH
Department of Medicine, Baylor College of Medicine, Houston, TX 77030, USA.
Woo
J S
JS
Perng
C L
CL
Go
M F
MF
Graham
D Y
DY
El-Zaatari
F A
FA
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000502:physiology; D005798:Genes, Bacterial / Q000235:genetics*; D015698:Genomic Library; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000032:analysis / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional / Q000235:genetics / Q000502:physiology; D009154:Mutation / Q000235:genetics / Q000502:physiology; D017422:Sequence Analysis, DNA; D012967:Sodium Dodecyl Sulfate; D014534:UDPglucose 4-Epimerase / Q000235:genetics*
D004269:DNA, Bacterial; D008070:Lipopolysaccharides; D012967:Sodium Dodecyl Sulfate; D014534:UDPglucose 4-Epimerase; C020551:galactose epimerase