Role of ultraviolet A-induced oxidative DNA damage in apoptosis via loss of mitochondrial membrane potential and caspase-3 activation.
UVA has been implicated in multistage photocarcinogenesis. HPLC analysis revealed that the 7,8-dihydro-8-oxo-2'-deoxyguanosine content of DNA in HL-60 cells increased immediately after UVA irradiation. Pulsed field gel electrophoresis showed that cellular DNA strand breakage was induced at 1 h after UVA irradiation. Flow cytometry showed that mitochondrial membrane potential (delta psi m) decreased time-dependently in cells exposed to UVA. The activity of caspase-3 and DNA ladder formation increased at 2 h after UVA irradiation. These results suggest that UVA irradiation induces oxidative DNA damage, followed by the loss of delta psi m and subsequent activation of caspase-3, resulting in apoptosis.
Tada-Oikawa
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Department of Hygiene, Mie University School of Medicine, Japan.
Oikawa
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Kawanishi
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D000080242:8-Hydroxy-2'-Deoxyguanosine; D017209:Apoptosis / Q000528:radiation effects*; D053148:Caspase 3; D020169:Caspases; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D016521:Electrophoresis, Gel, Pulsed-Field; D004789:Enzyme Activation / Q000528:radiation effects; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D008564:Membrane Potentials / Q000528:radiation effects*; D008928:Mitochondria / Q000528:radiation effects*; D009842:Oligopeptides / Q000378:metabolism; D018384:Oxidative Stress / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*
D009842:Oligopeptides; D000080242:8-Hydroxy-2'-Deoxyguanosine; C505407:CASP3 protein, human; D053148:Caspase 3; D020169:Caspases; D003546:Cysteine Endopeptidases; D003849:Deoxyguanosine