title: In vitro osteoinduction of demineralized bone.
abstract: Among numerous available materials for osseous repair and reconstruction, those presenting osteoinductive characteristics and promoting bone regeneration are preferable. Fresh autologous bone is one of the most effective, but it has some disadvantages and risks. Demineralized bone matrix (DBM) is considered to be a valid alternative, because it seems to show osteogenic potential, ascribed to the presence of bone morphogenetic proteins. In addition it can be prepared without difficulty and preserved without losing osteoinductive properties. The aim of the study was to evaluate the osteoinductive ability of xenogenic DBM, by testing DBM powder obtained from rabbit long bones, in cell culture of murine fibroblasts, alone or associated with electromagnetic field (EMF), that are known to exhibit biologic effects on cells: in particular they are used in orthopedics to improve bone formation. At the end of experiment, alkaline phosphatase (ALP) activity, calcium levels and cell proliferation and morphology were evaluated. A statistically significant stimulation of ALP activity and cell proliferation and a morphological change of fibroblasts were found. The results obtained show how DBM and EMF have different effects on cells, and that together they have synergic action toward bone induction.
authors.affiliation: Department of Experimental Surgery, Orthopaedic Institutes Rizzoli, Bologna, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Torricelli
authors.forename: M
authors.initials: M
authors.lastname: Fini
authors.forename: G
authors.initials: G
authors.lastname: Giavaresi
authors.forename: R
authors.initials: R
authors.lastname: Giardino
mesh_terms: D000818:Animals; D018775:Bone Demineralization Technique; D001857:Bone Matrix / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D004574:Electromagnetic Fields; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000528:radiation effects; D011208:Powders; D011817:Rabbits
chemical_list: D011208:Powders