title: Transcription factor NF-E2 is essential for the polyploidization of a human megakaryoblastic cell line, Meg-J.
abstract: Transcription factors regulating the process of megakaryocyte development remain largely unclarified. To clarify them further, we used a human megakaryoblastic cell line, Meg-J, which showed prominant polyploidization and augmented platelet glycoprotein (GP) Ib expression after incubation with thrombopoietin (TPO, c-mpl ligand) and K252a (an indolocarbasole derivative). Under these conditions, we analyzed the expression of the transcription factors and observed that the expression of NF-E2 p45, but not those of GATA-1, GATA-2, Tal-1/SCL, Evi-1, and MafK, was increased after TPO and K252a stimulation. Gel-shift assay confirmed the enhanced binding activity to the NF-E2 site. The abolishment of NF-E2 p45 with NF-E2 antisense oligomers inhibited TPO plus K252a-induced polyploidization. These findings suggest that NF-E2 p45 is essential for the polyploidization of megakaryocytic cells.
authors.affiliation: Department of Hematology, Tokyo Women's Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Teramura
authors.forename: K
authors.initials: K
authors.lastname: Ito
authors.forename: K
authors.initials: K
authors.lastname: Iwabe
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: H
authors.initials: H
authors.lastname: Mizoguchi
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins; D002227:Carbazoles / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D006801:Humans; D026121:Indole Alkaloids; D007947:Leukemia, Megakaryoblastic, Acute / Q000235:genetics* / Q000473:pathology*; D000074008:MDS1 and EVI1 Complex Locus Protein; D050985:MafK Transcription Factor; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D011123:Polyploidy; D011518:Proto-Oncogene Proteins; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D013926:Thrombopoietin / Q000494:pharmacology; D014157:Transcription Factors / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D014407:Tumor Cells, Cultured
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002227:Carbazoles; D004268:DNA-Binding Proteins; D050981:Erythroid-Specific DNA-Binding Factors; D050982:GATA1 Transcription Factor; D050989:GATA2 Transcription Factor; D026121:Indole Alkaloids; D000074008:MDS1 and EVI1 Complex Locus Protein; D050985:MafK Transcription Factor; D050983:NF-E2 Transcription Factor; D050984:NF-E2 Transcription Factor, p45 Subunit; D016376:Oligonucleotides, Antisense; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D013926:Thrombopoietin; D014157:Transcription Factors; C494688:GATA1 protein, human; C494711:GATA2 protein, human; C494695:MAFK protein, human; C063575:MECOM protein, human; C494692:NFE2 protein, human; C067143:TAL1 protein, human; C049985:staurosporine aglycone