Enhanced broth media for selective growth of Vibrio vulnificus.
Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.
Hsu
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Department of Food Science and Human Nutrition, University of Florida, Gainesville 32611, USA.
Wei
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Tamplin
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010461:Peptones / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014733:Vibrio / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D003470:Culture Media; D010461:Peptones; D012965:Sodium Chloride