Membrane fusion is induced by a distinct peptide sequence of the sea urchin fertilization protein bindin.
Fertilization in the sea urchin is mediated by the membrane-associated acrosomal protein bindin, which plays a key role in the adhesion and fusion between sperm and egg. We have investigated the structure/function relationship of an 18-amino acid peptide fragment "B18," which represents the minimal membrane binding motif of the protein and resembles a putative fusion peptide. The peptide was found to mimic the behavior of its parent protein bindin with respect to (a) its high affinity for lipid bilayers, (b) the ability to aggregate and fuse vesicles, (c) the binding of Zn2+ by a histidine-rich motif, (d) the tendency to self-assemble, and (e), as indicated earlier, the adhesion to cell surface polysaccharides. Fluorescence and light scattering assays were used here to monitor peptide-induced lipid mixing, leakage, and aggregation of large unilamellar sphingomyelin/cholesterol vesicles. For these activities, B18 requires the presence of Zn2+ ions, with which it forms oligomeric complexes and assumes a partially alpha-helical conformation, as observed by circular dichroism. We conclude that aggregation and fusion involves a "trans-complex" between peptides on apposing vesicles that are connected by Zn2+ bridges.
Ulrich
A S
AS
Institute of Molecular Biology, University of Jena, Winzerlaer Strasse 10, 07745 Jena, Germany.
Otter
M
M
Glabe
C G
CG
Hoekstra
D
D
D000595:Amino Acid Sequence; D000818:Animals; D002412:Cations; D002942:Circular Dichroism; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008297:Male; D008561:Membrane Fusion / Q000502:physiology*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000378:metabolism / Q000502:physiology*; D011485:Protein Binding; D011487:Protein Conformation; D011956:Receptors, Cell Surface; D012617:Sea Urchins; D013084:Sperm-Ovum Interactions
D002412:Cations; D006023:Glycoproteins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor