[Effect of prenatal gamma-irradiation on functional properties of rat liver adenylate cyclase].
In the present work the effects of long-term prenatal gamma-irradiation (0.5 Gy) on the glucagon signalling through adenylyl cyclase have been investigated. In utero gamma-irradiation resulted in the increase of basal and GTP-stimulated adenylyl cyclase activity, whereas, the adenylyl cyclase response to glucagon was essentially reduced. The comparison of kinetic constants estimated from dose-response data for GTP and glucagon suggests that prenatal chronic irradiation prompted (i) the decrease in rate of GTP hydrolysis on Gs-protein; (ii) the reduction of glucagon potency to accelerate the exchange GDP for GTP on Gs-protein.
Chubanov
V S
VS
Department of Biochemistry, School of Biology, Belarus State University, Minsk, Belarus. biochem@bio.bsu.unibel.by
Rogov
Iu I
IuI
Sholukh
M V
MV
Konoplia
E F
EF
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D004789:Enzyme Activation; D005260:Female; D005720:Gamma Rays; D005934:Glucagon / Q000096:biosynthesis / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D015398:Signal Transduction / Q000528:radiation effects
D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases
Vliianie vnutriutrobnogo gamma-oblucheniia na funktsional'nye svoĭstva adenilattsiklazy pecheni krys.