Production specific antigens from Trichinella spiralis using a continuous elution method and isoelectric focusing.
Immunodominant antigens of 45-53 kDa (one band per fraction) were obtained from excretory/secretory (E/S) and somatic products of infective-larvae of Trichinella spiralis using a continuous-elution method. They were further resolved by isoelectric focusing into different isoforms (45 kDa: pI4.47, 5.09, 5.47 and 5.86; 47 kDa: pI4.72 and 4.97; 53 kDa: pI4.86, 5.11, 5.44 and 5.78). In immunoblotting, the isoforms of pI 5.09, 5.86, 4.97, 5.44 and 5.78 did not cross-react with antisera against Trichuris suis, Metastrongylus apri, Gnathostoma hispidum and Stephanurus dentatus. Hence, they have the potential to serve as specific antigens for the serodiagnosis of trichinellosis.
Leung
R K
RK
Department of Zoology, University of Hong Kong, Hong Kong.
Ko
R C
RC
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification*; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015151:Immunoblotting; D016056:Immunodominant Epitopes / Q000302:isolation & purification; D007525:Isoelectric Focusing; D007814:Larva / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000662:veterinary
D000947:Antigens, Helminth; D016056:Immunodominant Epitopes