Establishment of prostatic cell line "Pro9ad" from a p53-deficient mouse.
BACKGROUND
We demonstrated that p53-deficiency is sufficient for immortalization of fetal uterine cells. In the present study, we further extended our previous observations to prostate tissues from a young p53-deficient adult mouse.


METHODS
Cell lines were established from the ventral prostate of a p53-deficient male mouse and maintained in medium containing 10% heat-inactivated fetal calf serum supplemented with insulin (10 microg/ml), transferrin (10 microg/ml), cholera toxin (10 ng/ml), and selenium (10(-8) M).


RESULTS
Pro9ad, one of the lines established, exhibits a typical epithelial morphology in culture. Despite the possession of androgen receptors, the growth of Pro9ad was not stimulated by 5alpha-dihydrotestosterone. Hepatocyte growth factor (HGF) slightly stimulated proliferation, whereas fibroblast growth factor-1 (FGF-1), keratinocyte growth factor (KGF), and platelet-derived growth factor AB (PDGF-AB) had no stimulating effect on growth. However, FGF-2, epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) accelerated proliferation in a dose-dependent manner. EGF and IGF-1 additively stimulated growth.


CONCLUSIONS
These results suggest that Pro9ad shares characteristics in common with primary prostatic epithelial cells despite p53-deficiency, and that p53-deficiency alone allows establishment of clonal cell lines of the prostate epithelium. Furthermore, the prostates of p53-deficient mice are useful sources for obtaining cell lines.
Hanazono
M
M
Department of Clinical Research, Ichihara Hospital, School of Medicine, Teikyo University, Chiba, Japan.
Nakagawa
E
E
Aizawa
S
S
Tomooka
Y
Y
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D016895:Culture Media, Serum-Free; D013196:Dihydrotestosterone / Q000494:pharmacology; D004347:Drug Interactions; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005312:Fetal Blood; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology; D006358:Hot Temperature; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D008297:Male; D051379:Mice; D011467:Prostate / Q000166:cytology* / Q000378:metabolism; D011944:Receptors, Androgen / Q000032:analysis / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency*
D003470:Culture Media; D016895:Culture Media, Serum-Free; D011944:Receptors, Androgen; D016159:Tumor Suppressor Protein p53; D013196:Dihydrotestosterone; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I