Studies of the presence of the virulence factors, adhesion, invasion, intracellular multiplication and toxin formation in salmonellas of different origin.
Salmonellas of different origin were classified into two groups (11 strains of common serovars which had been isolated from organs of calves having died from salmonellosis and 18 strains belonging to rare serovars which showed uncommon metabolic characteristics and had been isolated from spices and spiced foods). The strains were examined with regard to different virulence parameters. All salmonellas investigated possessed the genetic information on invasion (invA) and toxin formation (stn). They adhered equally well to epithelial cells, could penetrate into these and survive and multiply inside the cells. The formation of toxic substances could be detected in all strains after co-cultivation with epithelial cells in the CHO-K1 test. Significant differences between the groups of strains could be demonstrated only for the invasion of epithelial cell monolayers. Since adhesion, invasion and the ability of intracellular survival and multiplication as well as toxin formation constitute virulence parameters of salmonellas, it must be assumed that also the Salmonella serotypes studied which have been rarely observed epidemiologically constitute a risk for humans.
Dinjus
U
U
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena.
Hänel
I
I
Rabsch
W
W
Helmuth
R
R
D000818:Animals; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics*; D016466:CHO Cells; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002460:Cell Line; D006224:Cricetinae; D051381:Rats; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism / Q000472:pathogenicity*; D012481:Salmonella Infections, Animal / Q000382:microbiology*; D014774:Virulence
D001426:Bacterial Proteins; C075518:invA protein, Bacteria