title: Physical map of human 6p21.2-6p21.3: region flanking the centromeric end of the major histocompatibility complex.
abstract: We have physically mapped and cloned a 2.5-Mb chromosomal segment flanking the centromeric end of the major histocompatibility complex (MHC). We characterized in detail 27 YACs, 144 cosmids, 51 PACs, and 5 BACs, which will facilitate the complete genomic sequencing of this region of chromosome 6. The contig contains the genes encoding CSBP, p21, HSU09564 serine kinase, ZNF76, TCP-11, RPS10, HMGI(Y), BAK, and the human homolog of Tctex-7 (HSET). The GLO1 gene was mapped further centromeric in the 6p21.2-6p21.1 region toward TCTE-1. The gene order of the GLO1-HMGI(Y) segment in respect to the centromere is similar to the gene order in the mouse t-chromosome distal inversion, indicating that there is conservation in gene content but not gene order between humans and mice in this region. The close linkage of the BAK and CSBP genes to the MHC is of interest because of their possible involvement in autoimmune disease.
authors.affiliation: Division of Medical and Molecular Genetics, United Medical and Dental School of Guy's and St. Thomas', Guy's Hospital, London SE1 9RT, UK.
authors.forename: N
authors.initials: N
authors.lastname: Tripodis
authors.forename: R
authors.initials: R
authors.lastname: Mason
authors.forename: S J
authors.initials: SJ
authors.lastname: Humphray
authors.forename: A F
authors.initials: AF
authors.lastname: Davies
authors.forename: J A
authors.initials: JA
authors.lastname: Herberg
authors.forename: J
authors.initials: J
authors.lastname: Trowsdale
authors.forename: D
authors.initials: D
authors.lastname: Nizetic
authors.forename: G
authors.initials: G
authors.lastname: Senger
authors.forename: J
authors.initials: J
authors.lastname: Ragoussis
mesh_terms: D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics; D002503:Centromere / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D002876:Chromosomes, Bacterial; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020451:Contig Mapping; D003360:Cosmids / Q000138:chemical synthesis; D015246:Deoxyribonuclease EcoRI / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008285:Major Histocompatibility Complex / Q000235:genetics*; D020161:Physical Chromosome Mapping
chemical_list: D001426:Bacterial Proteins; D015246:Deoxyribonuclease EcoRI; C044171:endodeoxyribonuclease BSSHII; C035041:endodeoxyribonuclease MluI; D015252:Deoxyribonucleases, Type II Site-Specific