title: Transforming growth factor-beta1, insulin-like growth factors, and insulin-like growth factor binding proteins in ovarian follicular fluid are differentially regulated by the type of ovarian hyperstimulation used for in vitro fertilization.
abstract: OBJECTIVE
  To determine the effects of hMG and highly purified FSH on follicular production of the ovarian growth factors transforming growth factor-beta1 (TGF-beta1), insulin-like growth factors I and II (IGF-I and IGF-II), and insulin-like growth factor binding proteins-1 and -3 (IGFBP-1 and IGFBP-3).
  
  
  METHODS
  Controlled clinical study.
  
  
  METHODS
  University IVF program.
  
  
  METHODS
  One hundred twenty women who were <38 years old and had a >3-year duration of infertility in their present relationship participated in the study.
  
  
  METHODS
  Follicular fluid and matched serum were collected at oocyte pick-up and analyzed for growth factors and E2 with the use of ELISA and RIA.
  
  
  METHODS
  Levels of TGF-beta1, IGF-I. IGF-II, IGFBP-1 and IGFBP-3 in follicular fluid and levels of E2 in serum were measured.
  
  
  RESULTS
  Compared with highly purified FSH, ovarian hyperstimulation with hMG produced lower levels of TGF-beta1 and IGF-I and higher levels of IGFBP-1. Levels of IGF-II and IGFBP-3 were similar with the 2 treatments.
  
  
  CONCLUSIONS
  In patients undergoing IVF, the follicular expression of TGF-beta1, IGF-I, and IGFBP-1 was regulated differently by highly purified FSH compared with a preparation containing FSH and LH in a 1:1 ratio (hMG). The results indicate that FSH and LH control ovarian production of these growth factors differentially.
authors.affiliation: Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden. GABRIEL.FRIED@FYFA.KI.SE
authors.forename: G
authors.initials: G
authors.lastname: Fried
authors.forename: H
authors.initials: H
authors.lastname: Wramsby
authors.forename: M
authors.initials: M
authors.lastname: Tally
mesh_terms: D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000502:physiology*; D010062:Ovulation Induction; D011863:Radioimmunoassay; D013002:Somatomedins / Q000378:metabolism*; D013268:Stimulation, Chemical; D013799:Theca Cells / Q000378:metabolism / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D016212:Transforming Growth Factor beta; D004958:Estradiol