A prospective study of matrix metalloproteinases in proliferative vitreoretinopathy.
OBJECTIVE
The migration, proliferation, differentiation, and adhesion of cells and other cellular functions are influenced by the surrounding extracellular matrix, in normal and wound-healing conditions. The matrix metalloproteinases (MMPs) are a family of enzymes that degrade and remodel the extracellular matrix and, thus, play a central role in the wound-healing process. Proliferative vitreoretinopathy (PVR), a wound-healing process in the retina, is a major cause of the failure of retinal detachment surgery. The role of MMPs in the pathobiology of PVR is unknown. We have investigated the presence of MMPs in the vitreous of patients with retinal detachment and the predictive value of MMPs for the future development of PVR.


METHODS
A prospective study was conducted on 140 consecutive patients with a rhegmatogenous retinal detachment in whom vitrectomy was considered necessary because of a giant retinal tear and the presence of preoperative PVR, among other reasons. Vitreous samples were obtained and analyzed by zymography for the presence of MMPs. The patients were then followed up for the development of postoperative PVR (mild and severe).


RESULTS
Two species of MMPs were detected in the vitreous: MMP-2 and MMP-9. MMP-2 was detected in all of the vitreous samples obtained, whereas MMP-9 was found in only 64 (47%) of 136 samples. The levels of MMPs detected were not significantly associated with the presence of preoperative PVR (P > 0.05), but they were significantly associated (P < 0.05) with the development of postoperative PVR (mild and severe).


CONCLUSIONS
The results from this prospective study suggest that MMPs may be an important predictor and may also play a role in the development of postoperative PVR.
Kon
C H
CH
Wound Healing Research Unit, Department of Pathology, Institute of Ophthalmology, Moorfields Eye Hospital, London, United Kingdom.
Occleston
N L
NL
Charteris
D
D
Daniels
J
J
Aylward
G W
GW
Khaw
P T
PT
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017364:Collagenases / Q000378:metabolism; D005109:Extracellular Matrix / Q000201:enzymology; D005260:Female; D005500:Follow-Up Studies; D018093:Gelatinases / Q000378:metabolism; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000378:metabolism*; D008875:Middle Aged; D008970:Molecular Weight; D011446:Prospective Studies; D012163:Retinal Detachment / Q000209:etiology / Q000601:surgery; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000150:complications / Q000201:enzymology* / Q000601:surgery; D014822:Vitreous Body / Q000201:enzymology
D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9