Synthetic peptides of human papillomavirus type 18 E6 harboring HLA-A2.1 motif can induce peptide-specific cytotoxic T-cells from peripheral blood mononuclear cells of healthy donors.
To identify cytotoxic T-cell (CTL) epitopes against human papillomavirus type 18 (HPV 18) E6 protein that might be useful for developing peptide-based vaccine against HPV 18 infection, 18 peptides which possibly contain CTL epitopes were selected on the basis of previously described human leukocyte antigen (HLA)-A2.1-binding motif and chemically synthesized. In the binding assay of the synthetic peptides, 8 out of 18 synthetic peptides enhanced the expression of HLA-A2.1 molecules on T2 cell surface, which implies that these peptides were able to bind the HLA molecules. Those peptides having good binding affinity to HLA-A2.1 were tested for their ability to activate CTLs which were isolated from peripheral blood mononuclear cells (PBMCs) of healthy blood donors and to kill the target T2 cells pulsed with the same peptide. Five out of eight tested peptides activated CTLs and killed the target cells.
Yoon
H
H
Virus/Oncology Research Unit, Korea Research Institute of Bioscience and Biotechnology, KIST, Yusong, Taejon, South Korea.
Chung
M K
MK
Min
S S
SS
Lee
H G
HG
Yoo
W D
WD
Chung
K T
KT
Jung
N P
NP
Park
S N
SN
D001665:Binding Sites; D002460:Cell Line; D002478:Cells, Cultured; D004268:DNA-Binding Proteins; D015789:HLA-A2 Antigen / Q000276:immunology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009856:Oncogene Proteins, Viral / Q000138:chemical synthesis / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D004268:DNA-Binding Proteins; C052603:E6 protein, Human papillomavirus type 18; D015789:HLA-A2 Antigen; D009856:Oncogene Proteins, Viral; D010455:Peptides