title: [Obtention and characterization of murine beta-NGF. Application in a model of cerebral aging].
abstract: BACKGROUND
  beta-NGF is a basic protein of 118 aminoacids which acts are a trophic factor for sensory and sympathetic neurons of the peripheral nervous system, and on cholinergic neurons of the anterior basal cerebrum.
  
  
  OBJECTIVE
  In view of the functional effect of beta-HGF and its possibilities as a therapeutic agent in neurodegenerative disease, including Alzheimer's disease in this study our aim was to obtain, characterize and show the main results of the application of beta-NGFm in a model of cerebral ageing in rats with cognitive disorders.
  
  
  METHODS
  For the obtention of beta-NGFm we followed Mobley's method as modified by Ebendal and used mouse submaxillary gland as a source of raw material. The characterization studies were carried out by application of seven techniques which allowed physicochemical characterization and demonstration of the biological activity of the product. Application of beta-NGF obtained under these conditions was carried out in a mode of cerebral ageing and the effects of treatment were assessed by conduct studies, measurement of the activity of the enzyme acetyl cholinesterase and study of neural plasticity.
  
  
  CONCLUSIONS
  Characterization studies carried out on the beta-NGFm showed that the protein obtained consists of a mixture of molecules of beta-NGFm which are intact at their extreme N-Terminal, and molecules which have lost the octapeptide of the N-terminal position and show some modification increasing hydrophobicity. All these species were recognized immunologically by the specific antibody anti-NGFm and showed biological activity.
authors.affiliation: Centro Internacional de Restauración Neurológica CIREN, La Habana, Cuba.
authors.forename: M R
authors.initials: MR
authors.lastname: Castellanos
authors.forename: G
authors.initials: G
authors.lastname: Moya
authors.forename: R
authors.initials: R
authors.lastname: Cruz
authors.forename: L
authors.initials: L
authors.lastname: Lorigados
authors.forename: C I
authors.initials: CI
authors.lastname: Fernández
authors.forename: C
authors.initials: C
authors.lastname: Díaz
authors.forename: K
authors.initials: K
authors.lastname: de la Cuétara
authors.forename: J
authors.initials: J
authors.lastname: Bergado
authors.forename: L
authors.initials: L
authors.lastname: Francis
authors.forename: N
authors.initials: N
authors.lastname: Pavón
authors.forename: E
authors.initials: E
authors.lastname: González
authors.forename: R
authors.initials: R
authors.lastname: Ramos
mesh_terms: D000375:Aging / Q000502:physiology*; D000544:Alzheimer Disease / Q000503:physiopathology*; D000818:Animals; D001921:Brain / Q000502:physiology*; D004195:Disease Models, Animal; D016332:Fetal Tissue Transplantation; D006624:Hippocampus / Q000601:surgery; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000502:physiology*; D009473:Neuronal Plasticity / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012688:Septum Pellucidum / Q000196:embryology / Q000637:transplantation
chemical_list: D009414:Nerve Growth Factors
vernacular_title: Obtención y caracterización del beta-NGF murino. Aplicación en un modelo de envejecimiento cerebral.