DNA triple-helix formation on nucleosome-bound poly(dA).poly(dT) tracts.
We have used DNase I and hydroxyl-radical footprinting to examine the formation of intermolecular DNA triple helices on nucleosome-bound DNA fragments containing An.Tn tracts. We found that it is possible to form triplexes on these nucleosome-bound DNAs, but the stability of the complexes depends on the orientation of the A tract with respect to the protein surface. Hydroxyl-radical cleavage of these complexes suggests that the DNA fragments are still associated with the nucleosome. However, the phased cleavage pattern is lost in the vicinity of the triplex, suggesting that the DNA has locally moved away from the protein surface.
Brown
P M
PM
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, U.K.
Fox
K R
KR
D001483:Base Sequence; D004247:DNA / Q000378:metabolism*; D018983:DNA Footprinting; D017665:Hydroxyl Radical / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000378:metabolism*; D011067:Poly dA-dT / Q000378:metabolism*
D009707:Nucleosomes; D011067:Poly dA-dT; D017665:Hydroxyl Radical; D004247:DNA