title: Tissue dose, DNA adducts, oxidative DNA damage and CYP1A-immunopositive proteins in mussels exposed to waterborne benzo[a]pyrene.
abstract: A collaborative study was performed on Mediterranean mussels (Mytilus galloprovincialis) exposed to a wide dose-range (0.5-1000 ppb) of benzo[a]pyrene (B[a]P). We selected this model polycyclic aromatic hydrocarbon in order to confirm the formation of a specific DNA adduct, previously detected in gill DNA, and to clarify the in vivo effects of this mutagenic chemical requiring host-metabolism in mussels. B[a]P concentration reached consistently higher values in the digestive gland than in other analyzed tissues of mussels exposed to B[a]P for 2 or 3 days. With the exception of some values at 1000 ppb of B[a]P. DNA adduct levels increased significantly with the dose in gills and digestive gland and ranged from 0.054 to 0.789 adducts per 10(8) nucleotides (mean values per dose-point). Conversely, more complex dose-response relationships were found by detecting in parallel the levels of an oxidative DNA lesion (8-OHdG) and of CYP1A-immunopositive proteins (the latter measured in the digestive gland only). Overall, the formation of DNA adducts, the evidence of oxidative DNA damage, and changes in CYP1A-immunopositive protein levels support the hypothesis that B[a]P can induce DNA damage in mussels through a number of different molecular mechanisms.
authors.affiliation: Department of Biology, Univ. of Padova, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Canova
authors.forename: P
authors.initials: P
authors.lastname: Degan
authors.forename: L D
authors.initials: LD
authors.lastname: Peters
authors.forename: D R
authors.initials: DR
authors.lastname: Livingstone
authors.forename: R
authors.initials: R
authors.lastname: Voltan
authors.forename: P
authors.initials: P
authors.lastname: Venier
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity*; D049872:Bivalvia / Q000187:drug effects*; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D018736:DNA Adducts / Q000032:analysis*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D004064:Digestive System / Q000737:chemistry / Q000201:enzymology; D005880:Gills / Q000737:chemistry / Q000201:enzymology; D007558:Italy; D009153:Mutagens / Q000633:toxicity*; D012623:Seawater; D014877:Water Pollution, Chemical
chemical_list: D018736:DNA Adducts; D009153:Mutagens; D001564:Benzo(a)pyrene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003577:Cytochrome P-450 Enzyme System; D003849:Deoxyguanosine