title: Stimulation of interleukin-6 production in human dental pulp cells by peptidoglycans from Lactobacillus casei.
abstract: Interleukin-6 (IL-6), which is a multifunctional cytokine, has an important role in acute and chronic inflammation. The peptidoglycan (PG) was purified from Lactobacillus casei, which was a Gram-positive bacteria frequently isolated from deep carious lesions and suspected to be a pathogen of pulpitis. In this study, the effects of PG on the production of IL-6 in human dental pulp cells were examined. PG stimulated IL-6 production in a time- and dose-dependent manner. Reverse transcriptase-polymerase chain reaction experiments showed that the increase was dependent on the enhancement of IL-6 mRNA levels. These findings suggest that Gram-positive bacteria, such as L. casei, from carious lesions, might be involved in developing pulpitis through the stimulation of IL-6 production.
authors.affiliation: Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Matsushima
authors.forename: E
authors.initials: E
authors.lastname: Ohbayashi
authors.forename: H
authors.initials: H
authors.lastname: Takeuchi
authors.forename: S
authors.initials: S
authors.lastname: Hosoya
authors.forename: Y
authors.initials: Y
authors.lastname: Abiko
authors.forename: M
authors.initials: M
authors.lastname: Yamazaki
mesh_terms: D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism / Q000494:pharmacology*; D011671:Pulpitis / Q000209:etiology; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
chemical_list: D015850:Interleukin-6; D010457:Peptidoglycan; D012333:RNA, Messenger