Information from the water stripe in TOCSY experiments on systems with exchangeable protons.
The water "stripes" of the TOCSY maps of aqueous solutions of sucrose, of a 15-aminoacid peptide, and of several of the constituent aminoacids are shown to contain correlations at the resonance frequencies of protons which are scalar coupled to OH or NH protons which exchange with the solvent. Theoretical analysis of chemical exchange during the spin-lock period in TOCSY elucidates the origin of these correlations, and shows that their intensities vary with the duration of the spin-lock period and with the exchange rate.
Nakashima
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Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
McClung
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Kotovych
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D000596:Amino Acids / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D006859:Hydrogen / Q000737:chemistry; D017665:Hydroxyl Radical / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008956:Models, Chemical; D009584:Nitrogen / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D011522:Protons; D012997:Solvents / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry; D013912:Threonine / Q000737:chemistry; D013997:Time Factors; D014443:Tyrosine / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D000596:Amino Acids; D010455:Peptides; D011522:Protons; D012997:Solvents; D014867:Water; D013912:Threonine; D017665:Hydroxyl Radical; D014443:Tyrosine; D013395:Sucrose; D002244:Carbon; D006859:Hydrogen; D008239:Lysine; D003545:Cysteine; D009584:Nitrogen