title: Flow cytometric analysis of cell cycle fractions in oral leukoplakia.
abstract: Flow cytometric analysis of cell cycle fractions and DNA ploidy was performed on 39 biopsy specimens of oral leukoplakia. The aneuploidy rate of these leukoplakias was 9/39 (23.1%) and the mean DNA index of the aneuploid lesions was 1.34. The aneuploidy rate was significantly higher in severely dysplastic lesions (8/17) than in mildly dysplastic (1/15, P<0.02) and nondysplastic (0/7, P<0.05) lesions. No significant differences in the percentages of each cell cycle fraction were seen between the diploid and the aneuploid leukoplakias. However, the S-phase fraction of the severely dysplastic lesions (23.0%) among the diploid leukoplakias was higher than those of the mildly dysplastic (12.4%) and nondysplastic (15.5%) lesions, and the difference between the severely dysplastic and the mildly dysplastic lesions was statistically significant (P<0.001). These results suggest that flow cytometric analysis of cell cycle fractions and DNA ploidy might offer additional information for assessing the malignant potential of oral leukoplakias.
authors.affiliation: First Department of Oral Surgery, Hokkaido University School of Dentistry, Sapporo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Saito
authors.forename: S
authors.initials: S
authors.lastname: Mizuno
authors.forename: K
authors.initials: K
authors.lastname: Notani
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
authors.forename: I
authors.initials: I
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Shindoh
authors.forename: T
authors.initials: T
authors.lastname: Kohgo
mesh_terms: D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000473:pathology; D002453:Cell Cycle; D016009:Chi-Square Distribution; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics* / Q000473:pathology*
chemical_list: D004273:DNA, Neoplasm