title: Definition of T cell epitopes within the 19 kDa carboxylterminal fragment of Plasmodium yoelii merozoite surface protein 1 (MSP1(19)) and their role in immunity to malaria.
abstract: MSP1(19) is one of the leading malaria vaccine candidates. However, the mechanism of protection is not clear. To determine whether MSP1(19)-specific effector T cells can control parasitaemia, we analysed the specificity of T cells induced following immunization with recombinant forms of P. yoelii MSP1(19) and asked whether they could protect mice. There was no evidence that effector T cells were capable of protecting since: (1) immunization of mice with yMSP1(19), but not defined epitopes, was able to induce protection; and (2) long term MSP1(19)-specific CD4+ T cell lines were incapable of adoptively transferring protection. In contrast, priming mice with the T cell epitopes resulted in a rapid anamnestic antibody response to MSP1(19) after either challenge with MSP1(19) or parasite. Thus, MSP1(19) contains multiple T cell epitopes but such epitopes are the targets of helper T cells for antibody response but not of identified effector T cells capable of controlling parasitaemia.
authors.affiliation: Laboratory of Parasitic Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: J H
authors.initials: JH
authors.lastname: Tian
authors.forename: M F
authors.initials: MF
authors.lastname: Good
authors.forename: C
authors.initials: C
authors.lastname: Hirunpetcharat
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: I T
authors.initials: IT
authors.lastname: Ling
authors.forename: D
authors.initials: D
authors.lastname: Jackson
authors.forename: J
authors.initials: J
authors.lastname: Cooper
authors.forename: J
authors.initials: J
authors.lastname: Lukszo
authors.forename: J
authors.initials: J
authors.lastname: Coligan
authors.forename: J
authors.initials: J
authors.lastname: Ahlers
authors.forename: A
authors.initials: A
authors.lastname: Saul
authors.forename: J A
authors.initials: JA
authors.lastname: Berzofsky
authors.forename: A A
authors.initials: AA
authors.lastname: Holder
authors.forename: L H
authors.initials: LH
authors.lastname: Miller
authors.forename: D C
authors.initials: DC
authors.lastname: Kaslow
mesh_terms: D019264:Adoptive Transfer; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry / Q000276:immunology*; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology; D002460:Cell Line; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000276:immunology*; D005260:Female; D008213:Lymphocyte Activation; D008288:Malaria / Q000276:immunology*; D017780:Malaria Vaccines / Q000737:chemistry / Q000276:immunology*; D020066:Merozoite Surface Protein 1; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008969:Molecular Sequence Data; D010967:Plasmodium yoelii / Q000276:immunology*; D011498:Protein Precursors / Q000737:chemistry / Q000276:immunology*; D015800:Protozoan Proteins / Q000737:chemistry / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D000954:Antigens, Surface; D018984:Epitopes, T-Lymphocyte; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins