Effects of IL-1 receptor accessory protein on IL-1 binding.
Interleukin-1 (IL-1) is an important pro-inflammatory cytokine that exerts its diverse biological functions by binding to a receptor complex consisting of two transmembrane proteins, type I IL-1 receptor (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). Both receptor chains are indispensable for IL-1 signaling, but only IL-1RI is able to bind the cytokine. The effects of IL-1RAcP on IL-1 binding are unclear. This study shows that although expression of IL-1RAcP does not alter the equilibrium dissociation constant of IL-1, it has an effect on the non-equilibrium binding modus, most likely due to changes in on/off rates. This defines an additional function of IL-1RAcP: it stabilizes the active IL-1 receptor complex.
Wesche
H
H
Institut für Molekularpharmakologie, Medizinische Hochschule Hannover, Germany.
Resch
K
K
Martin
M U
MU
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002460:Cell Line; D007375:Interleukin-1 / Q000378:metabolism*; D053577:Interleukin-1 Receptor Accessory Protein; D008024:Ligands; D051379:Mice; D009500:Neutralization Tests; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000494:pharmacology*; D017472:Receptors, Interleukin-1 / Q000378:metabolism*; D053573:Receptors, Interleukin-1 Type I; D015398:Signal Transduction; D013601:T-Lymphocytes / Q000166:cytology
C507767:Il1rap protein, mouse; D007375:Interleukin-1; D053577:Interleukin-1 Receptor Accessory Protein; D008024:Ligands; D011506:Proteins; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I