title: Determination of N3-methyl-5'-deoxy-5-fluorouridine, a novel metabolite of doxifluridine, in body fluids by high performance liquid chromatography.
abstract: A high performance liquid chromatography method for the determination of N3-methyl-5'-deoxy-5-fluorouridine, a possible metabolic product of the anticancer pro-drug 5'-deoxy-5-fluorouridine, in human serum and urine is described. Sample treatment involved addition of internal standard (5-bromouracil) and protein precipitation with ammonium sulphate (serum samples) followed by liquid-liquid extraction with ethyl acetate-isopropanol (90:10, v/v). The average recovery at 0.5 mg ml-1 level was (80 +/- 4%). A linear response extending over two decades of concentration was observed. Detection limits of 50 and 100 ng ml-1 were obtained in serum and urine, respectively.
authors.affiliation: Dipartimento di Chimica, Università degli Studi della Basilicata, Potenza, Italy.
authors.forename: C G
authors.initials: CG
authors.lastname: Zambonin
authors.forename: S
authors.initials: S
authors.lastname: Mastrolitti
authors.forename: F
authors.initials: F
authors.lastname: Palmisano
mesh_terms: D000645:Ammonium Sulfate; D000970:Antineoplastic Agents / Q000097:blood* / Q000652:urine; D001976:Bromouracil; D002851:Chromatography, High Pressure Liquid; D005467:Floxuridine / Q000031:analogs & derivatives* / Q000097:blood* / Q000652:urine; D006801:Humans; D011355:Prodrugs / Q000032:analysis*; D012015:Reference Standards; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000970:Antineoplastic Agents; C103213:N(3)-methyl-5'-deoxy-5-fluorouridine; D011355:Prodrugs; D005467:Floxuridine; D001976:Bromouracil; D000645:Ammonium Sulfate; C025034:doxifluridine