A new vaccinia virus intermediate transcription factor.
Transcription of the vaccinia virus genome is mediated by a virus-encoded multisubunit DNA-dependent RNA polymerase in conjunction with early-, intermediate-, and late-stage-specific factors. Previous studies indicated that two virus-encoded proteins (capping enzyme and VITF-1) and one unidentified cellular protein (VITF-2) are required for specific transcription of an intermediate promoter template in vitro. We have now extensively purified an additional virus-induced intermediate transcription factor with a native mass of approximately 100 kDa.
Sanz
P
P
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0445, USA.
Moss
B
B
D006367:HeLa Cells; D006801:Humans; D014157:Transcription Factors / Q000737:chemistry / Q000302:isolation & purification*; D014616:Vaccinia virus / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry / Q000302:isolation & purification*
D014157:Transcription Factors; D014764:Viral Proteins