title: Sustained modifications of protein metabolism in various tissues in a rat model of long-lasting sepsis.
abstract: 1. Sepsis was induced in rats by an intravenous injection of live bacteria. Infected and pair-fed animals were studied before the infection, in an acute septic phase (day 2 post-infection), in a chronic septic phase (day 6) and in a late septic phase (day 10). Protein synthesis rates were measured in vivo after administration of a flooding dose of L[1-13C]valine. 2. During the acute phase, muscle protein loss associated with infection resulted from both a decrease in protein synthesis and an increase in proteolysis. During the chronic phase and the late phase, the increase of proteolysis in infected rats as compared with pair-fed animals persisted, worsening muscle atrophy. Skin protein synthesis rates were not significantly modified by infection. However, skin protein content decreased 6 and 10 days after infection, suggesting an increased proteolysis in response to sepsis. 3. Protein synthesis in liver of infected rats was twice that of pair-fed animals. Liver protein synthesis remained elevated in infected rats compared with pair-fed animals until day 10. Hypoalbuminaemia and high plasma concentrations of fibrinogen were evident at all periods studied. alpha 2-Macroglobulin and alpha 1-acid glycoprotein reached peak concentrations during the acute phase (concentrations increased 50 times in infected rats). On day 10, the levels of these proteins were still about 12-fold higher. 4. Protein synthesis rates were significantly increased in the digestive tract and lung of infected rats compared with pair-fed groups on days 2 and 6, but were similar in the two groups on day 10 post-infection. The fractional protein synthesis rate was increased 3-fold over the entire experimental period in the spleen. 5. The results show that sepsis stimulates protein synthesis in various tissues over a long time, and that skin, like muscle, can provide amino acids to the rest of the body.
authors.affiliation: Centre de Recherche en Nutrition Humaine, Ceyrat, France.
authors.forename: D
authors.initials: D
authors.lastname: Breuillé
authors.forename: M
authors.initials: M
authors.lastname: Arnal
authors.forename: F
authors.initials: F
authors.lastname: Rambourdin
authors.forename: G
authors.initials: G
authors.lastname: Bayle
authors.forename: D
authors.initials: D
authors.lastname: Levieux
authors.forename: C
authors.initials: C
authors.lastname: Obled
mesh_terms: D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D002908:Chronic Disease; D007413:Intestinal Mucosa / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D013154:Spleen / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D011506:Proteins