Chemokine receptor expression in cultured glia and rat experimental allergic encephalomyelitis.
Chemokines are a group of pro-inflammatory peptides that mediate leukocyte migration and activation. Several members of the chemokine family have been shown to be synthesized by cells of the central nervous system (CNS). To begin to address the role of chemokine receptors in CNS physiology, we identified, by molecular cloning techniques, the rat orthologs of the chemokine receptors, CCR2, CCR3, CCR5, and CXCR4. CCR2 and CCR5 expression was detected in rat spleen, lung, kidney, thymus and macrophages; CCR5 mRNA was also detected in rat brain. Primary cultures of rat microglia expressed CCR5 mRNA that was regulated by IFN-gamma, while both cultured astrocytes and microglia were found to contain mRNA for CXCR4 and CX3CR1. Induction of experimental allergic encephalomyelitis (EAE) in the rat was accompanied by increased levels of CCR2, CCR5, CXCR4, and CX3CR1 mRNAs in the lumbar spinal cords of animals displaying clinical signs of the disease. These data identify the rat orthologs of chemokine receptors and demonstrate that brain, spinal cord, and cultured glial cells express chemokine receptors that can be regulated both in vitro and in vivo.
Jiang
Y
Y
Department of Pharmacology and Therapeutics, College of Medicine, University of Florida, Gainesville 32610-0267, USA.
Salafranca
M N
MN
Adhikari
S
S
Xia
Y
Y
Feng
L
L
Sonntag
M K
MK
deFiebre
C M
CM
Pennell
N A
NA
Streit
W J
WJ
Harrison
J K
JK
D000595:Amino Acid Sequence; D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000276:immunology; D001923:Brain Chemistry / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007668:Kidney / Q000166:cytology; D008297:Male; D017628:Microglia / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D054390:Receptors, CCR2; D054397:Receptors, CCR3; D054398:Receptors, CCR4; D019707:Receptors, Chemokine / Q000235:genetics*; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology; D014982:Xenopus laevis
C515334:CCR2 protein, human; C515350:CCR3 protein, human; C515354:CCR4 protein, human; C515336:Ccr2 protein, rat; C515352:Ccr3 protein, rat; C515356:Ccr4 protein, rat; D012333:RNA, Messenger; D054390:Receptors, CCR2; D054397:Receptors, CCR3; D054398:Receptors, CCR4; D019707:Receptors, Chemokine; D019204:GTP-Binding Proteins