Clonal nature of enterotoxigenic Escherichia coli serotype O6:H16 revealed by randomly amplified polymorphic DNA analysis.
The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.
Pacheco
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Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Brazil. biafp@ibccf.biof.ufrj.br
Soares
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de Almeida
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Viboud
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GI
Binsztein
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N
Ferreira
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D001427:Bacterial Toxins / Q000096:biosynthesis*; D015373:Bacterial Typing Techniques; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins; D006801:Humans; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D014774:Virulence
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli