Cloning and mapping of the mouse Gpx2 gene encoding gastrointestinal glutathione peroxidase.
Gastrointestinal glutathione peroxidase (GSHPx-GI) is an enzyme expressed in intestinal epithelial cells and may reduce hydroperoxides generated from the ingested diet. We isolated a genomic clone containing the mouse Gpx2 gene encoding 190 amino acids of GSHPx-GI. This gene is composed of two exons and an intron. The nucleotide sequence of the coding region was 89.9% identical with that of the human GPX2 gene. A TGA opal codon predicted to encode a selenocysteine was identified at codon 40. A genomic clone containing a pseudogene for the Gpx2 gene was also isolated. The nucleotide sequence of the pseudogene was 98.3% identical with that of the mouse Gpx2 gene and showed characteristics of a processed pseudogene. Linkage analysis using backcross mouse progeny indicated the mouse Cpx2 gene and its pseudogene to be located on mouse chromosomes 12 and 7, respectively.
Tsuji
T
T
Faculty of Agriculture, Okayama University, Japan.
Watanabe
Y
Y
Katoh
H
H
Sato
K
K
Kunieda
T
T
D000595:Amino Acid Sequence; D000821:Animal Feed; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003062:Codon; D003433:Crosses, Genetic; D015252:Deoxyribonucleases, Type II Site-Specific; D004032:Diet; D005091:Exons; D005260:Female; D008040:Genetic Linkage; D005979:Glutathione Peroxidase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D007438:Introns; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011544:Pseudogenes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D017279:Selenocysteine / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid
D003062:Codon; D011994:Recombinant Proteins; D017279:Selenocysteine; D005979:Glutathione Peroxidase; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific