Requirement of membrane-proximal amino acids in the carboxyl-terminal tail for expression of the rat AT1a angiotensin receptor.
A series of deletion mutants was created to analyze the function of the membrane-proximal region of the cytoplasmic tail of the rat type 1a (AT1a) angiotensin receptor. In transiently transfected COS-7 cells, the truncated mutant receptors showed a progressive decrease in surface expression, with no major change in binding affinity for the peptide antagonist, [Sar1,Ile8]angiotensin II. In parallel with the decrease in receptor expression, a progressive decrease in angiotensin II-induced inositol phosphate responses was observed. Alanine substitutions in the region 307-311 identified the highly conserved phenylalanine309 and adjacent lysine residues as significant determinants of AT1a receptor expression.
Gáborik
Z
Z
Department of Physiology, Semmelweis University of Medicine, Budapest, Hungary.
Mihalik
B
B
Jayadev
S
S
Jagadeesh
G
G
Catt
K J
KJ
Hunyady
L
L
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D007295:Inositol Phosphates / Q000378:metabolism; D008958:Models, Molecular; D009130:Muscle, Smooth / Q000378:metabolism*; D016296:Mutagenesis; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D017433:Protein Structure, Secondary; D051381:Rats; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin / Q000096:biosynthesis* / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012504:Saralasin / Q000378:metabolism; D017384:Sequence Deletion; D014162:Transfection
D007295:Inositol Phosphates; D044140:Receptor, Angiotensin, Type 1; D011945:Receptors, Angiotensin; D011994:Recombinant Proteins; D012504:Saralasin