Genetic engineering of potato for broad-spectrum protection against virus infection.
Transgenic potato plants expressing mutant alleles of PLRV ORF4, the gene for the movement protein pr17 of this luteovirus, were generated for broad-range protection against virus infection. When tested for protection against infection by PLRV, all transgenic lines showed a significant reduction of virus antigen. Potato lines accumulating N- or C-terminally extended PLRV pr17 mutant proteins were resistant to infection by the unrelated potato viruses PVY and PVX. Transgenic lines that did not express protein despite high transcript levels failed to exhibit virus resistance.
Tacke
E
E
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Salamini
F
F
Rohde
W
W
D000483:Alleles; D000956:Antigens, Viral / Q000032:analysis; D005818:Genetic Engineering; D017793:Luteovirus / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics; D019176:Potyviridae / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics / Q000821:virology*; D014158:Transcription, Genetic / Q000235:genetics
D000956:Antigens, Viral