title: Comparison of throat swabs with sputum specimens for the detection of Chlamydia pneumoniae antigen by direct immunofluorescence.
abstract: OBJECTIVE
  To compare throat swabs with sputum specimens for Chlamydia pneumoniae antigen detection.
  
  
  METHODS
  During a one year period, sputum and throat swabs from 50 patients over 15 years of age with acute or persisting lower respiratory tract infection were examined for C pneumoniae antigen by direct immunofluorescence.
  
  
  RESULTS
  C pneumoniae antigen was detected in 18/50 patients (36.0%) from sputum, throat swab, or both. Paired sputum and throat swabs were received from 35/50 patients (70.0%). C pneumoniae antigen was detected in either or both specimens from 14/35 patients (40.0%). Of the 14 positive patients, both specimens were positive in nine (64.3%), throat swab only in four (28.6%), and sputum only in one (7.1%). Of the remaining 15 patients from whom only a single specimen was sent, a further three of eight throat swabs and one of seven sputum specimens were positive. There was no statistically significant difference between the results obtained from the two types of specimen.
  
  
  CONCLUSIONS
  Throat swabs may be as good as sputum for the detection of C pneumoniae antigen.
authors.affiliation: Department of Microbiology, Fife Area Laboratory, Kirkcaldy, UK.
authors.forename: P
authors.initials: P
authors.lastname: Garnett
authors.forename: O
authors.initials: O
authors.lastname: Brogan
authors.forename: C
authors.initials: C
authors.lastname: Lafong
authors.forename: C
authors.initials: C
authors.lastname: Fox
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis*; D002690:Chlamydia Infections / Q000175:diagnosis*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D008875:Middle Aged; D010614:Pharynx / Q000382:microbiology*; D011446:Prospective Studies; D012141:Respiratory Tract Infections / Q000175:diagnosis; D013048:Specimen Handling / Q000379:methods; D013183:Sputum / Q000382:microbiology*
chemical_list: D000942:Antigens, Bacterial