title: Chromosomal and gene amplification in diffuse large B-cell lymphoma.
abstract: Chromosomal translocations leading to deregulation of specific oncogenes characterize approximately 50% of cases of diffuse large B-cell lymphomas (DLBL). To characterize additional genetic features that may be of value in delineating the clinical characteristics of DLBL, we studied a panel of 96 cases at diagnosis consecutively ascertained at the Memorial Sloan-Kettering Cancer Center (MSKCC) for incidence of gene amplification, a genetic abnormality previously shown to be associated with tumor progression and clinical outcome. A subset of 20 cases was subjected to comparative genomic hybridization (CGH) analysis, which identified nine sites of chromosomal amplification (1q21-23, 2p12-16, 8q24, 9q34, 12q12-14, 13q32, 16p12, 18q21-22, and 22q12). Candidate amplified genes mapped to these sites were selected for further analysis based on their known roles in lymphoid cell and lymphoma development, and/or history of amplification in tumors. Probes for six genes, which fulfilled these criteria, REL (2p12-16), MYC (8q24), BCL2 (18q21), GLI, CDK4, and MDM2 (12q13-14), were used in a quantitative Southern blotting analysis of the 96 DLBL DNAs. Each of these genes was amplified (four or more copies) with incidence ranging from 11% to 23%. This analysis is consistent with our previous finding that REL amplification is associated with extranodal presentation. In addition, BCL2 rearrangement and/or REL, MYC, BCL2, GLI, CDK4, and MDM2 amplification was associated with advanced stage disease. These data show, for the first time, that amplification of chromosomal regions and genes is a frequent phenomenon in DLBL and demonstrates their potential significance in lymphomagenesis.
authors.affiliation: Cell Biology Program and the Departments of Pathology and Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
authors.forename: P H
authors.initials: PH
authors.lastname: Rao
authors.forename: J
authors.initials: J
authors.lastname: Houldsworth
authors.forename: K
authors.initials: K
authors.lastname: Dyomina
authors.forename: N Z
authors.initials: NZ
authors.lastname: Parsa
authors.forename: J C
authors.initials: JC
authors.lastname: Cigudosa
authors.forename: D C
authors.initials: DC
authors.lastname: Louie
authors.forename: L
authors.initials: L
authors.lastname: Popplewell
authors.forename: K
authors.initials: K
authors.lastname: Offit
authors.forename: S C
authors.initials: SC
authors.lastname: Jhanwar
authors.forename: R S
authors.initials: RS
authors.lastname: Chaganti
mesh_terms: D002874:Chromosome Mapping; D002877:Chromosomes, Human; D005784:Gene Amplification; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D014178:Translocation, Genetic