Solid-phase cDNA library construction, a versatile approach.
A rapid and versatile method for cDNA library construction was developed. It is based on conventional cDNA library synthesis including all enzymatic steps usually required, but is performed on a solid support. The cDNA is immobilised via a biotin residue to streptavidin coupled magnetic beads, which allows rapid and easy to perform changes of buffers and enzymes. Therefore, it combines speed (library construction within a single day) with high quality libraries, making it ideally suited for most purposes.
Roeder
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University of Hamburg, Zoological Institute, Department of Neurophysiology, Martin-Luther-King-Platz 3,D-20146 Hamburg, Germany. roeder@zoologie.uni-hamburg.de
D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors
D018076:DNA, Complementary