title: The effect of albumin priming solution on platelet activation during experimental long-term perfusion.
abstract: The objective of this study was to evaluate the effect of albumin priming on platelet consumption and activation during long-term perfusion. Two identical in vitro extracorporeal membrane oxygenation circuits were used; one was primed with Ringer's solution containing human serum albumin, the other with Ringer's solution only. Fresh heparinized human blood was pooled, divided between the two systems and circulated for 24 h at 37 degrees C. Platelet count, plasma concentration of betathromboglobulin (BTG), platelet membrane density of glycoprotein (GP) Ib and of GPIIb/IIIa were assayed before the start and at 0.5, 1, 3, 12 and 24 h of perfusion. In total, seven experiments were performed. We found that during the first hour of perfusion, slightly higher platelet counts (p = 0.058) and lower BTG values (p = 0.0005) were observed in the circuits primed with albumin, compared to the control circuits. No statistically significant differences were observed for the platelet membrane expression of GPIb and GPIIb/IIIa. We conclude that albumin priming appears to transiently prevent platelet consumption and activation during long-term perfusion.
authors.affiliation: Department of Paediatrics, Sahlgrenska University Hospital/Ostra, Göteborg, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Adrian
authors.forename: K
authors.initials: K
authors.lastname: Mellgren
authors.forename: M
authors.initials: M
authors.lastname: Skogby
authors.forename: L G
authors.initials: LG
authors.lastname: Friberg
authors.forename: G
authors.initials: G
authors.lastname: Mellgren
authors.forename: H
authors.initials: H
authors.lastname: Wadenvik
mesh_terms: D015199:Extracorporeal Membrane Oxygenation; D006801:Humans; D010477:Perfusion; D015539:Platelet Activation / Q000187:drug effects*; D010976:Platelet Count / Q000187:drug effects; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000032:analysis; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000032:analysis; D012709:Serum Albumin / Q000494:pharmacology*; D012996:Solutions / Q000494:pharmacology; D001620:beta-Thromboglobulin / Q000032:analysis
chemical_list: D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019038:Platelet Glycoprotein GPIb-IX Complex; D012709:Serum Albumin; D012996:Solutions; D001620:beta-Thromboglobulin