A simple and rapid method for measuring unconjugated capsular polysaccharide (PRP) of Haemophilus influenzae type b in PRP-tetanus toxoid conjugate vaccine.
The authors developed a simple and rapid method for quantitation of free capsular polysaccharide of Haemophilus influenzae type b (polyribosyl ribitol phosphate, PRP) in PRP-tetanus toxoid conjugate vaccine based on acid precipitation of tetanus toxoid (TT). Acid hydrolysis of PRP during the assay was not detected. The conditions used in the assay did not precipitate unconjugated PRP or adipic acid dihydrazide derivatized PRP. The method was highly reliable, reproducible and sensitive. The accuracy of the assay was confirmed by spiking known amounts of unconjugated PRP to PRP-TT conjugate preparations. A PRP-TT preparation, incubated at 37 degrees C for 6 months showing most of the PRP as unconjugated (87% determined by this method), was not immunogenic in mice for the PRP component even after two injections. In contrast, the same preparation held at 4 degrees C for 20 months, showing 17% unconjugated PRP, induced IgG antibodies to PRP which were boosted after second injection. Therefore, this method is very useful to evaluate the stability of PRP-TT conjugate vaccine. The assay may be useful for characterizing other polysaccharide-protein conjugate vaccines.
Guo
Y Y
YY
Massachusetts Biologic Laboratories, Boston 02130, USA.
Anderson
R
R
McIver
J
J
Gupta
R K
RK
Siber
G R
GR
D000818:Animals; D011232:Chemical Precipitation; D003840:Deoxycholic Acid; D018073:Haemophilus Vaccines / Q000737:chemistry*; D019741:Haemophilus influenzae type b; D006863:Hydrogen-Ion Concentration; D051379:Mice; D011134:Polysaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013745:Tetanus Toxoid / Q000737:chemistry*; D018074:Vaccines, Conjugate / Q000737:chemistry*
D018073:Haemophilus Vaccines; C055753:Haemophilus influenza type b polysaccharide vaccine-tetanus toxin conjugate; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013745:Tetanus Toxoid; D018074:Vaccines, Conjugate; D003840:Deoxycholic Acid; C008984:polyribitol phosphate