title: Interaction of human Arp2/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation.
abstract: Actin filament assembly at the cell surface of the pathogenic bacterium Listeria monocytogenes requires the bacterial ActA surface protein and the host cell Arp2/3 complex. Purified Arp2/3 complex accelerated the nucleation of actin polymerization in vitro, but pure ActA had no effect. However, when combined, the Arp2/3 complex and ActA synergistically stimulated the nucleation of actin filaments. This mechanism of activating the host Arp2/3 complex at the L. monocytogenes surface may be similar to the strategy used by cells to control Arp2/3 complex activity and hence the spatial and temporal distribution of actin polymerization.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94143, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Welch
authors.forename: J
authors.initials: J
authors.lastname: Rosenblatt
authors.forename: J
authors.initials: J
authors.lastname: Skoble
authors.forename: D A
authors.initials: DA
authors.lastname: Portnoy
authors.forename: T J
authors.initials: TJ
authors.lastname: Mitchison
mesh_terms: D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D001704:Biopolymers; D002462:Cell Membrane / Q000378:metabolism; D015638:Cytochalasin D / Q000494:pharmacology; D003598:Cytoskeletal Proteins; D006801:Humans; D007700:Kinetics; D008089:Listeria monocytogenes / Q000378:metabolism*; D008565:Membrane Proteins / Q000737:chemistry / Q000378:metabolism*; D008854:Microscopy, Electron
chemical_list: C495926:ACTR2 protein, human; C495928:ACTR3 protein, human; D051377:Actin-Related Protein 2; D051378:Actin-Related Protein 3; D000199:Actins; D001426:Bacterial Proteins; D001704:Biopolymers; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C072980:actA protein, Listeria monocytogenes; D015638:Cytochalasin D