Response to isoproterenol of rabbit detrusor strips following exposure to NSAIDs.
To ascertain whether NSAIDs affect the physiological mechanisms involved in bladder detrusor muscle motility we investigated the influence of indomethacin, ibuprofen and acetyl salicylic acid on the beta-adrenergic-evoked response in isolated male-rabbit detrusor strips. Incubation with indomethacin for 60 min significantly reduced the tissue response to isoproterenol (up to 10.8+/-4.9%), entirely abolished spontaneous contractions and caused a fall in basal tone. Ibuprofen significantly inhibited the tissue response to isoproterenol (up to 25.5+/-5.8%), abolished spontaneous contractions and caused a fall in basal tone. After the spasmogen KCl had been added to restore basal tone, the highest indomethacin and ibuprofen concentrations still significantly inhibited the isoproterenol-evoked response, indomethacin being the most active compound. Acetyl salicylic acid, at the doses used, neither inhibited spontaneous contractions nor changed basal tone whereas it significantly inhibited tissue response to isoproterenol (up to 69. 6+/-4.6%). Our findings in rabbit detrusor strips show that NSAIDs affect the beta-adrenergic-evoked response. They probably do so mainly through mechanisms other then inhibition of prostaglandin production.
Bolle
P
P
Institute of Pharmacology and Pharmacognosy, University of Rome, P. le Aldo MORO 5, Rome, 00185, Italy.
Tucci
P
P
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009543:Nifedipine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology
D000318:Adrenergic beta-Agonists; D000894:Anti-Inflammatory Agents, Non-Steroidal; D011943:Receptors, Adrenergic, beta; D011189:Potassium Chloride; D009543:Nifedipine; D007545:Isoproterenol