title: Fusarium graminearum A 3/5 as a novel host for heterologous protein production.
abstract: We describe a novel fungal expression system which utilizes the Quorn myco-protein fungus Fusarium graminearum A 3/5. A transformation system was developed for F. graminearum and was used to introduce the coding and regulatory regions of a trypsin gene from Fusarium oxysporum. The protein was efficiently expressed, processed and secreted by the recombinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive the expression of a cellulase gene from Scytalidium thermophilum and a lipase gene from Thermomyces lanuginosus in F. graminearum.
authors.affiliation: Novo Nordisk Biotech, Inc., Davis, California 95616, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Royer
authors.forename: D L
authors.initials: DL
authors.lastname: Moyer
authors.forename: S G
authors.initials: SG
authors.lastname: Reiwitch
authors.forename: M S
authors.initials: MS
authors.lastname: Madden
authors.forename: E B
authors.initials: EB
authors.lastname: Jensen
authors.forename: S H
authors.initials: SH
authors.lastname: Brown
authors.forename: C C
authors.initials: CC
authors.lastname: Yonker
authors.forename: J A
authors.initials: JA
authors.lastname: Johnston
authors.forename: E J
authors.initials: EJ
authors.lastname: Golightly
authors.forename: W T
authors.initials: WT
authors.lastname: Yoder
mesh_terms: D001483:Base Sequence; D002480:Cellulase / Q000235:genetics; D005285:Fermentation; D005670:Fusarium / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D008049:Lipase / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D012045:Regulatory Sequences, Nucleic Acid; D014162:Transfection; D014357:Trypsin / Q000235:genetics
chemical_list: D011506:Proteins; D011994:Recombinant Proteins; D008049:Lipase; D002480:Cellulase; D014357:Trypsin