title: Secretory component production by polarized epithelial cells from the human female reproductive tract.
abstract: At mucosal surfaces, the polymeric Ig receptor (pIgR) is responsible for transporting polymeric IgA across epithelial cells. The purpose of this study was to determine whether normal epithelial cells from the female reproductive tract form tight junctions and produce secretory component, the external domain of the pIgR. Uterine, cervical and vaginal tissues from women at different stages of the menstrual cycle and following menopause were used to prepare purified epithelial cell sheets, which were cultured in cell chambers. Transepithelial resistance was measured and the media from apical and basolateral compartments assayed for secretory component. Secretory component produced by uterine epithelial cells accumulated preferentially in apical compartment and correlated with increased transepithelial resistance. Seeding as epithelial sheets at 1 x 10(6) cells/cm2 of matrix coated cell chambers was required for growth. Epithelial cells from endo-cervix and ecto-cervix, but not the vagina, also showed preferential production and release of secretory component into the apical chamber. In conclusion, normal epithelial cells from the human female reproductive tract grow to confluence, become polarized and produce secretory component. Our results suggest that uterine and cervical epithelial cells play a key regulatory role in the control of IgA transcytosis from tissue into secretions.
authors.affiliation: Department of Physiology, Dartmouth Medical School, New Hampshire 03756, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Fahey
authors.forename: S L
authors.initials: SL
authors.lastname: Humphrey
authors.forename: J E
authors.initials: JE
authors.lastname: Stern
authors.forename: C R
authors.initials: CR
authors.lastname: Wira
mesh_terms: D016764:Cell Polarity; D002478:Cells, Cultured; D046508:Culture Techniques / Q000379:methods; D004847:Epithelial Cells / Q000276:immunology*; D005260:Female; D005836:Genitalia, Female / Q000166:cytology / Q000276:immunology*; D006801:Humans; D007044:Hysterectomy; D008597:Menstrual Cycle / Q000276:immunology*; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D019056:Receptors, Polymeric Immunoglobulin / Q000378:metabolism*; D019108:Tight Junctions / Q000502:physiology
chemical_list: D019056:Receptors, Polymeric Immunoglobulin