title: KARP-1 is induced by DNA damage in a p53- and ataxia telangiectasia mutated-dependent fashion.
abstract: The KARP-1 (Ku86 Autoantigen Related Protein-1) gene, which is expressed from the human Ku86 autoantigen locus, appears to play a role in mammalian DNA double-strand break repair as a regulator of the DNA-dependent protein kinase complex. Here we demonstrate that KARP-1 gene expression is significantly up-regulated following exposure of cells to DNA damage. KARP-1 mRNA induction was completely dependent on the ataxia telangiectasia and p53 gene products, consistent with the presence of a p53 binding site within the second intron of the KARP-1 locus. These observations link ataxia telangiectasia, p53, and KARP-1 in a common pathway.
authors.affiliation: Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA.
authors.forename: K
authors.initials: K
authors.lastname: Myung
authors.forename: C
authors.initials: C
authors.lastname: Braastad
authors.forename: D M
authors.initials: DM
authors.lastname: He
authors.forename: E A
authors.initials: EA
authors.lastname: Hendrickson
mesh_terms: D001260:Ataxia Telangiectasia / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D004249:DNA Damage; D004265:DNA Helicases; D004260:DNA Repair; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D007438:Introns; D000072200:Ku Autoantigen; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D016159:Tumor Suppressor Protein p53; D004265:DNA Helicases; C106893:XRCC5 protein, human; D000072200:Ku Autoantigen