title: 237Np: oxidation state in vivo and chelation by multidentate catecholate and hydroxypyridinonate ligands.
abstract: Chemically, 237Np(V) is as toxic as U(VI), and radiologically, about as toxic as 239Pu. Depending on redox conditions in vivo, 237Np exists as weakly complexing Np(V) (NpO2+) or as Np(IV), which forms complexes as stable as those of Pu(IV). Ten multidentate catecholate (CAM) and hydroxypyridinonate (HOPO) ligands with great affinity for Pu(IV) were compared with CaNa3-DTPA for in vivo chelation of 237Np. Mice were injected intravenously with 237NpO2Cl: those in a kinetic study were killed 1 to 2880 min; in ligand studies, fed mice were injected intraperitoneally with a ligand 5, 60, or 1440 min after 237Np(V) (molar ratio 5.6 to 73), mice fasted for 16 h were gastrically intubated with a ligand 3 min after 237Np(V) (molar ratio 5.6 to 274), and all were killed 24 h after ligand administration; tissues and excreta were radioanalyzed. Rapid plasma clearance and urinary excretion of 237Np(V) resemble U(VI); deposition and early retention in skeleton and liver resemble Pu(IV). The x-ray absorption near edge structure spectroscopy (XANES) spectra of femora of 237Np(V)-injected mice, compared with spectra of Np(V) and Np(IV) from reference solids, showed predominantly Np(IV). Significant in vivo 237Np chelation was obtained with all of the HOPO and CAM ligands injected at molar ratio 22; the HOPO ligands reduced 237Np in skeleton, liver, and other soft tissue, on average, to 72, 25, and 25% of control, respectively, while CaNa3-DTPA was ineffective. Two HOPO ligands injected 60 min after 237Np (molar ratio 5.6) significantly reduced body and liver 237Np, and three HOPO ligands given orally (molar ratio > or = 73) significantly reduced body and liver 237Np, compared with controls. Combined with earlier work, these results indicate that: the dominant neptunium species circulating and excreted in urine is Np(V), while that in bone and liver deposits is Np(IV); Np(V) must be reduced to Np(IV) before it can be stably chelated; efficient decorporation of neptunium requires multidentate ligands that form exceptionally stable actinide(IV) chelates and facilitate Np(V) reduction.
authors.affiliation: Chemical Sciences Division, Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: P W
authors.initials: PW
authors.lastname: Durbin
authors.forename: B
authors.initials: B
authors.lastname: Kullgren
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: K N
authors.initials: KN
authors.lastname: Raymond
authors.forename: P G
authors.initials: PG
authors.lastname: Allen
authors.forename: J J
authors.initials: JJ
authors.lastname: Bucher
authors.forename: N M
authors.initials: NM
authors.lastname: Edelstein
authors.forename: D K
authors.initials: DK
authors.lastname: Shuh
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001417:Background Radiation; D001842:Bone and Bones / Q000378:metabolism; D002396:Catechols / Q000378:metabolism / Q000494:pharmacology; D002614:Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008024:Ligands; D051379:Mice; D009405:Neptunium / Q000008:administration & dosage / Q000493:pharmacokinetics*; D010084:Oxidation-Reduction; D011725:Pyridines / Q000378:metabolism / Q000494:pharmacology
chemical_list: D002396:Catechols; D002614:Chelating Agents; D008024:Ligands; D011725:Pyridines; C014047:hydroxypyridines; D009405:Neptunium