title: Differential inspiratory timing is genetically linked to mouse chromosome 3.
abstract: Genetic control of differential inspiratory timing (TI) at baseline has been previously demonstrated among inbred mouse strains. The inheritance pattern for TI between C3H/HeJ (C3; 188 +/- 3 ms) and C57BL/6J (B6; 111 +/- 2 ms) progenitors was consistent with a two-gene model. By using the strain distribution pattern for recombinant inbred strains derived from C3 and B6 progenitors, 100% concordance was established between TI phenotypes and DNA markers on mouse chromosome 3. This genotype-phenotype hypothesis was tested by typing 52 B6C3F2 (F2) progeny by using simple sequence repeat DNA markers (n = 21) polymorphic between C3 and B6 strains on mouse chromosome 3. Linkage analysis compared marker genotypes to baseline ventilatory phenotypes by computing log-likelihood values. A putative quantitative trait locus located in proximity to D3Mit119 was significantly associated with baseline TI phenotypes. At the peak (log-likelihood = 3.3), the putative quantitative trait locus determined 25% of the phenotypic variance in TI among F2 progeny. In conclusion, this genetic model of ventilatory characteristics demonstrated an important linkage between differential baseline TI and a candidate genomic region on mouse chromosome 3.
authors.affiliation: Department of Anesthesiology, The Johns Hopkins University, Baltimore, Maryland 21205, USA.
authors.forename: C G
authors.initials: CG
authors.lastname: Tankersley
authors.forename: D A
authors.initials: DA
authors.lastname: DiSilvestre
authors.forename: A E
authors.initials: AE
authors.lastname: Jedlicka
authors.forename: H M
authors.initials: HM
authors.lastname: Wilkins
authors.forename: L
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000235:genetics* / Q000502:physiology*; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D008040:Genetic Linkage / Q000235:genetics; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D016133:Polymerase Chain Reaction; D019655:Quantitative Trait, Heritable; D015656:Respiratory Mechanics / Q000235:genetics* / Q000502:physiology*
chemical_list: D004247:DNA