HLA-DR alleles are associated with IDDM, but not with impaired neutrophil chemotaxis in IDDM.
Insulin-dependent diabetes mellitus (IDDM) is a risk factor for periodontitis. Depressed neutrophil chemotaxis has been demonstrated in IDDM and in early-onset periodontitis (EOP). HLA-DR antigens are associated with both IDDM and periodontitis. This investigation sought to determine an association of HLA-DR3, -DR4, and -DR53 with impaired neutrophil chemotaxis in an IDDM sample. The neutrophil chemotaxis index of 41 diabetics and 27 controls was determined by a modified Boyden chamber method, and certain class II HLA genotypes were determined by polymerase chain-reaction amplification of genomic DNA by means of sequence-specific primers (PCR-SSP). The mean chemotaxis index of the diabetics was significantly less than that of the controls (p < or = 0.02). HLA-DR3 (p < or = 0.002), -DR4 (p < 0.003), and -DR53 (p < or = 0.001) were associated with IDDM. Neutrophil chemotaxis and glucose metabolism were not significantly correlated. None of the HLA-DR alleles was associated with impaired neutrophil chemotaxis. Therefore, the neutrophil chemotaxis defect of IDDM appears to be independent of these HLA-DR-associated genes.
Gustke
C J
CJ
US Public Health Service, Indian Health Service, Tohatchi Health Center, NM, USA.
Stein
S H
SH
Hart
T C
TC
Hoffman
W H
WH
Hanes
P J
PJ
Russell
C M
CM
Schuster
G S
GS
Watson
S C
SC
D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000209:etiology / Q000276:immunology; D000483:Alleles; D016022:Case-Control Studies; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D002648:Child; D004247:DNA / Q000032:analysis / Q000235:genetics; D017931:DNA Primers; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015194:Diffusion Chambers, Culture; D005260:Female; D005838:Genotype; D005947:Glucose / Q000378:metabolism; D006684:HLA-DR Antigens / Q000032:analysis / Q000235:genetics*; D015803:HLA-DR3 Antigen / Q000032:analysis / Q000235:genetics; D015804:HLA-DR4 Antigen / Q000032:analysis / Q000235:genetics; D059845:HLA-DRB4 Chains; D006801:Humans; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010518:Periodontitis / Q000209:etiology / Q000276:immunology; D016133:Polymerase Chain Reaction; D012307:Risk Factors
D017931:DNA Primers; D006684:HLA-DR Antigens; D015803:HLA-DR3 Antigen; D015804:HLA-DR4 Antigen; C050610:HLA-DR53; D059845:HLA-DRB4 Chains; D004247:DNA; D005947:Glucose