The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions.
The analysis of macromolecular interactions is an essential element of biomedical research. Flow cytometry is uniquely capable of making sensitive and quantitative measurements of molecular interactions. These measurements can be made in real time with subsecond kinetic resolution using purified biomolecules or living cells. Combined with automated sample handling, these features make flow cytometry a versatile and robust technology for the analysis of molecular interactions.
Nolan
J P
JP
National Flow Cytometry Resource and Life Sciences Division, Los Alamos National Laboratory, NM 87545, USA.
Sklar
L A
LA
D000954:Antigens, Surface / Q000032:analysis; D001331:Automation; D002477:Cells / Q000737:chemistry*; D004247:DNA / Q000032:analysis; D015195:Drug Design; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods; D019204:GTP-Binding Proteins / Q000737:chemistry; D008024:Ligands; D008958:Models, Molecular; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*
D000954:Antigens, Surface; D008024:Ligands; D010455:Peptides; D011506:Proteins; D004247:DNA; D019204:GTP-Binding Proteins