Similar mutational spectra in the HPRT gene of human and hamster cell lines after exposure to either low dose rate or high dose rate X-rays.
The dose rate at which cells are exposed to X-rays may influence the nature of induced mutations. To investigate this, the molecular spectra were determined at the HPRT gene in a hamster (V79) and a human (WI-L2-NS) cell line after the same total dose of X-rays has been administered at either a low dose rate (LDR; 3.33 mGy/min) or a high dose rate (HDR; 1.24-1.4 Gy/min) X-irradiation. Mutational spectra appeared similar, the fraction of mutants carrying deletions ranging between 59%-66% for the V79 strain and between 64%-75% for the WI-L2-NS strain, and independent of the irradiation conditions. The data indicate no effect of ongoing repair processes under LDR conditions on the kind of induced mutations in mammalian cells.
Colussi
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N
Department of Radiation Genetics and Chemical Mutagenesis (MGC), Leiden University, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands.
van Leeuwen
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X
Lohman
P H
PH
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D004247:DNA / Q000528:radiation effects*; D017931:DNA Primers; D004307:Dose-Response Relationship, Radiation; D005091:Exons; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D016296:Mutagenesis; D017354:Point Mutation; D017384:Sequence Deletion; D014965:X-Rays
D017931:DNA Primers; D004247:DNA; D007041:Hypoxanthine Phosphoribosyltransferase