Anthocyanin production of Glehnia littoralis callus cultures.
A stable callus line that produces anthocyanins was established from callus derived from a petiole of a Glehnia littoralis seedling and subcultured in the dark. The major anthocyanin which made up about 60% of the total anthocyanins was determined as cyanidin 3-O-(6-O-(6-O-(E)-feruloyl-beta-D-glucopyranosyl) -2-O-beta-D-xylopyranosyl-beta-D-glucopyranoside) by chemical and spectroscopic analyses. Anthocyanin contents in the cells cultured on B5 basal medium containing NAA (1 mg l-1), kinetin (0.01 mg l-1) and 3% sucrose reached 14% (dry wt basis) and the productivity has been sustained for 5 years.
Miura
H
H
School of Pharmaceutical Sciences, Nagasaki University, Japan.
Kitamura
Y
Y
Ikenaga
T
T
Mizobe
K
K
Shimizu
T
T
Nakamura
M
M
Kato
Y
Y
Yamada
T
T
Maitani
T
T
Goda
Y
Y
D000872:Anthocyanins / Q000096:biosynthesis*; D019661:Apiaceae / Q000378:metabolism*; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000502:physiology; D008969:Molecular Sequence Data; D010860:Pigments, Biological / Q000096:biosynthesis; D014018:Tissue Distribution
D000872:Anthocyanins; D010860:Pigments, Biological