title: Sulfhydryl reagent susceptibility in proteins with high sequence similarity--triosephosphate isomerase from Trypanosoma brucei, Trypanosoma cruzi and Leishmania mexicana.
abstract: The amino acid sequence of triosephosphate isomerase from Trypanosoma brucei, Trypanosoma cruzi, and Leishmania mexicana have an identity of 68%. Using the numbering system for the T. brucei enzyme, in their aligned sequences, the T. cruzi and leishmanial enzymes have cysteine residues at positions 14, 40, 117 and 126. T. brucei triosephosphate isomerase has cysteine residues at positions 14, 40 and 126, and a valine residue at position 117. Dithionitrobenzoic acid and methylmethane thiosulfonate inhibited the three enzymes, but T. cruzi triosephosphate isomerase was more than 100-fold more sensitive. The sensitivity of wild type triosephosphate isomerase from T. cruzi and T. brucei to the reagents was equal to that of the Cys117Val and Val117Cys mutant enzymes, respectively. Triosephosphate isomerases that have cysteine residues at positions 40 and 126, but lack a cysteine residue at position 14 are insensitive to methylmethane thiosulfonate. Thus, sulfhydryl reagents act on Cys14. At stoichiometric concentrations, the reagents inhibited the three enzymes as a consequence of structural alterations as measured by binding of 8-anilino-1-napthalenesulfonic acid to previously buried hydrophobic regions. However, the times for half-maximal alterations were 10 min, 15 hours and over 30 hours for T. cruzi, T. brucei and L. mexicana triosephosphate isomerase, respectively. The effect of pH on the action of the sulfhydryl reagents and molecular modeling showed no differences in the solvent accessibility of Cys14. As Cys14 forms part of the dimer interface, the data indicate that, in the three enzymes, barriers of different magnitude hinder the interaction between the sulfhydryl reagents and Cys14. The barrier is lower in T. cruzi triosephosphate isomerase which makes its dimer interface more susceptible for perturbation.
authors.affiliation: Departamento de Bioquímica Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, DF. mtuena@ifcsun1.ifisiol.unam.mx
authors.forename: G
authors.initials: G
authors.lastname: Garza-Ramos
authors.forename: N
authors.initials: N
authors.lastname: Cabrera
authors.forename: E
authors.initials: E
authors.lastname: Saavedra-Lira
authors.forename: M
authors.initials: M
authors.lastname: Tuena de Gómez-Puyou
authors.forename: P
authors.initials: P
authors.lastname: Ostoa-Saloma
authors.forename: R
authors.initials: R
authors.lastname: Pérez-Montfort
authors.forename: A
authors.initials: A
authors.lastname: Gómez-Puyou
mesh_terms: D000818:Animals; D003545:Cysteine; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D007700:Kinetics; D007894:Leishmania mexicana / Q000201:enzymology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000737:chemistry; D013439:Sulfhydryl Reagents / Q000494:pharmacology*; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000737:chemistry*; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D014349:Trypanosoma cruzi / Q000201:enzymology*
chemical_list: D011994:Recombinant Proteins; D013439:Sulfhydryl Reagents; D004228:Dithionitrobenzoic Acid; D008741:Methyl Methanesulfonate; D014305:Triose-Phosphate Isomerase; D003545:Cysteine