Structure and cross-reactivity of the O-specific polysaccharide of Proteus penneri strain 26, another neutral Proteus O-antigen containing 2-acetamido-2,6-dideoxy-L-glucose (N-acetyl-L-quinovosamine).
A neutral O-specific polysaccharide obtained from the lipopolysaccharide of Proteus penneri strain 26 was studied using sugar analysis and 1H and 13C NMR spectroscopy, including two-dimensional NMR techniques. The following structure of the trisaccharide repeating unit was established: -->6)-alpha-D-GlcpNAc-(1-->3)-alpha-L-QuipNAc-(1-->3)-alpha-D-Glcp NAc-(1--> where L-QuiNAc is 2-acetamido-2,6-dideoxy-L-glucose (N-acetyl-L-quinovosamine). Cross-reactivity of the Proteus penneri 26 anti-O serum with other strains of P. penneri isolated in Poland and USA and one strain of P. vulgaris is discussed.
Shashkov
A S
AS
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Arbatsky
N P
NP
Widmalm
G
G
Knirel
Y A
YA
Zych
K
K
Sidorczyk
Z
Z
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003429:Cross Reactions; D006386:Hemagglutination Tests; D006461:Hemolysis; D007106:Immune Sera; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D011511:Proteus / Q000737:chemistry / Q000276:immunology*; D011817:Rabbits
C113291:2-acetamido-2,6-dideoxy-glucose (N-acetyl-quinovosamine); D007106:Immune Sera; D019081:O Antigens; D000117:Acetylglucosamine