title: Evidence that whitefly-transmitted cowpea mild mottle virus belongs to the genus Carlavirus.
abstract: Two strains of whitefly-transmitted cowpea mild mottle virus (CPMMV) causing severe (CPMMV-S) and mild (CPMMV-M) disease symptoms in peanuts were collected from two distinct agro-ecological zones in India. The host-range of these strains was restricted to Leguminosae and Chenopodiaceae, and each could be distinguished on the basis of symptoms incited in different hosts. The 3'-terminal 2500 nucleotide sequence of the genomic RNA of both the strains was 70% identical and contains five open reading frames (ORFs). The first three (P25, P12 and P7) overlap to form a triple gene block of proteins, P32 encodes the coat protein, followed by P12 protein located at the 3' end of the genome. Genome organization and pair-wise comparisons of amino acid sequences of proteins encoded by these ORFs with corresponding proteins of known carlaviruses and potexviruses suggest that CPMMV-S and CPMMV-M are closely related to viruses in the genus Carlavirus. Based on the data, it is concluded that CPMMV is a distinct species in the genus Carlavirus.
authors.affiliation: Crop Protection Division, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.
authors.forename: R A
authors.initials: RA
authors.lastname: Naidu
authors.forename: S
authors.initials: S
authors.lastname: Gowda
authors.forename: T
authors.initials: T
authors.lastname: Satyanarayana
authors.forename: V
authors.initials: V
authors.lastname: Boyko
authors.forename: A S
authors.initials: AS
authors.lastname: Reddy
authors.forename: W O
authors.initials: WO
authors.lastname: Dawson
authors.forename: D V
authors.initials: DV
authors.lastname: Reddy
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017788:Carlavirus / Q000145:classification* / Q000235:genetics; D019663:Chenopodiaceae / Q000821:virology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D007887:Fabaceae / Q000821:virology; D016679:Genome, Viral; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010946:Plants, Medicinal; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary