Identification of a flunixin metabolite in camel by gas chromatography-mass spectrometry.
A flunixin metabolite, a hydroxylated product, has been identified in camel urine and plasma samples using gas chromatography-mass spectrometry (GC-MS) and GC-MS-MS in the electron impact and chemical ionization modes. Its major fragmentation pattern has been verified by GC-MS-MS in daughter ion and parent ion scan modes. The method could detect flunixin and its metabolite in camel urine after a single intravenous dose of 2.2 mg of flunixin/kg body weight for 96 and 48 h, respectively, which increases the reliability of antidoping control analysis.
Wasfi
I A
IA
Camelracing Forensic Laboratory, Forensic Science Laboratory, Abu Dhabi, United Arab Emirates.
Hadi
A A
AA
Alkatheeri
N A
NA
Barezaiq
I M
IM
ElGhazali
M
M
Boni
N S
NS
Zorob
O
O
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000097:blood* / Q000652:urine*; D002162:Camelus / Q000378:metabolism*; D003002:Clonixin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D007275:Injections, Intravenous; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods; D013997:Time Factors
D000894:Anti-Inflammatory Agents, Non-Steroidal; C014557:flunixin; D003002:Clonixin