title: Evaluation of membranous staining of mesothelioma.
abstract: Panels of immunohistochemical markers are often used to aid in categorizing malignant neoplasms involving the pleura. While epithelial membrane antigen (EMA) and human milk fat globule-2 (HMFG-2) are each reported to stain the majority of cases of adenocarcinoma and mesothelioma, authors have reported that the pattern of staining for these two antibodies can be a useful discriminant. Both are described as revealing a membranous pattern of staining for mesothelioma and a cytoplasmic staining pattern in adenocarcinoma. Immunostains were performed on 23 cases of malignant mesothelioma and 22 cases of adenocarcinoma. For EMA, 78% of mesotheliomas and 86% of adenocarcinomas stained positively, and 65% of mesotheliomas vs. 14% of adenocarcinomas exhibited a membranous staining pattern. For HMFG-2, 9% of mesotheliomas and 50% of adenocarcinomas stained positively, and 4% of mesotheliomas vs. 9% of adenocarcinomas exhibited a membranous staining pattern. Membranous staining for HMFG-2 was not a useful criterion. In this series, a membranous pattern of staining for EMA had a sensitivity of 65% and a specificity of 86% for the identification of malignant mesothelioma.
authors.affiliation: University of South Alabama Medical Center, Department of Pathology, Mobile, USA. jking@usamail.usouthal.edu
authors.forename: J A
authors.initials: JA
authors.lastname: King
authors.forename: J A
authors.initials: JA
authors.lastname: Tucker
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D003937:Diagnosis, Differential; D006017:Glycolipids / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D066292:Lipid Droplets; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology; D008566:Membranes / Q000378:metabolism / Q000473:pathology; D008654:Mesothelioma / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D018396:Mucin-1 / Q000378:metabolism; D010997:Pleural Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D012680:Sensitivity and Specificity; D013194:Staining and Labeling
chemical_list: D006017:Glycolipids; D006023:Glycoproteins; D018396:Mucin-1; C110367:milk fat globule