Detection of Japanese encephalitis virus by reverse transcription/polymerase chain reaction.
A simple and rapid reverse transcription/polymerase chain reaction (RT-PCR) assay for detection of Japanese encephalitis virus (JEV) envelope (E) gene sequences in various biological samples is described. The assay successfully amplified JEV E gene sequences from infected cell cultures, Aedes aegypti larvae, mosquitoes and mouse blood. The sensitivity of the assay was currently 1 ng of JEV RNA and could be increased up to 1 pg on the background of 1 microgram of cellular RNA by biotinylation of the PCR product, Southern blot analysis and streptavidin/alkaline phosphatase detection.
Paranjpe
S
S
National Institute of Virology, Pune, India.
Banerjee
K
K
D000330:Aedes / Q000821:virology; D000818:Animals; D002460:Cell Line; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000302:isolation & purification*; D007814:Larva; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D014158:Transcription, Genetic; D014759:Viral Envelope Proteins / Q000235:genetics*
D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C080628:glycoprotein E, Japanese encephalitis virus