Renal aminopeptidase activities in animal models of hypertension.
Aminopeptidase activity (AP) has been implicated in the metabolism of renal and circulating vasoactive peptides. This activity is involved in the pathogenia of hypertension, essentially in spontaneously hypertensive rats. However, no other animal models, which develop hypertension by other different ways, have been used to study the possible role of aminopeptidase activity. To investigate the role of this activity in the pathogenesis of hypertension, angiotensinase A activity (glutamyl-AP and aspartyl-AP), aminopeptidase M activity (alanyl-AP), aminopeptidase B activity (arginyl-AP), pyroglutamyl-AP, and cystinyl-AP were measured in the serum and kidney of two experimental animal models of renovascular hypertension: Goldblatt two-kidney one clip (G2K-1C) and low renal mass rats (LRM). No differences were found in serum levels of AP in LRM or G2K-1C in comparison with their respective controls. In LRM rats there was a significant decrease in membrane-bound angiotensinase A (glutamyl-AP), arginyl-AP and alanyl-AP activities. In G2K-1C rats there was a significant decrease in soluble and membrane-bound angiotensinase A activity (aspartyl-AP). Our results suggest that AP activities play a role in the regulation of renal vasoactive peptides, and respond differently depending on the cause of hypertension.
Ramírez
M
M
Unit of Physiology, University of Jaén, Spain. msanchez@ujaen.es
Prieto
I
I
Martinez
J M
JM
Vargas
F
F
Alba
F
F
D000626:Aminopeptidases / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D043384:Glutamyl Aminopeptidase; D006978:Hypertension, Renovascular / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D063208:Methionyl Aminopeptidases; D009929:Organ Size; D011751:Pyroglutamyl-Peptidase I / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar; D012995:Solubility
D000626:Aminopeptidases; C075408:cysteine aminopeptidase; D063208:Methionyl Aminopeptidases; C019721:aminopeptidase B; D043384:Glutamyl Aminopeptidase; D011751:Pyroglutamyl-Peptidase I