Purification and characterization of three acidic, cytotoxic phospholipases A2 from Indian cobra (Naja naja naja) venom.
Three acidic phospholipases A2 (NN-I2c-PLA2, NN-I2d-PLA2 and NN-I2c-PLA2) were purified by successive chromatography of Indian cobra (Naja naja naja) venom on CM-Sephadex C-25, Sephadex G-50 and QAE Sephadex A-25 columns. They have molecular weights of 13,000-14,500 by sodium dodecyl sulphate polyacrylamide gel electrophoresis. They showed tryptophan specific fluorescence emission spectra (approximately 345 nm). All the three phospholipases A2 were enzymatically highly active with specific activities 9-17 micromol min(-1) mg(-1). They were non-lethal to mice when injected intraperitoneally in doses up to 10 mg kg(-1) body weight. They induced edema in mouse foot pads and were cytotoxic to Ehrlich ascites tumour cells. They did not exhibit direct haemolytic and anticoagulant activities.
Rudrammaji
L M
LM
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Gowda
T V
TV
D000818:Animals; D004487:Edema / Q000139:chemically induced; D004546:Elapid Venoms / Q000201:enzymology*; D017815:Elapidae; D004912:Erythrocytes / Q000187:drug effects; D006801:Humans; D051379:Mice; D010741:Phospholipases A / Q000302:isolation & purification* / Q000633:toxicity; D054467:Phospholipases A2; D014407:Tumor Cells, Cultured
D004546:Elapid Venoms; D010741:Phospholipases A; D054467:Phospholipases A2