A simple technique to isolate DNA and supernatant of genital Mycoplasma hominis and Ureaplasma urealyticum.
Vaginal colonization with Mycoplasma hominis and Ureaplasma urealyticum has been implicated as a cause of prematurity. Several mechanisms to induce preterm labor have been discussed. The investigation of expressed and secreted enzymes requires a feasible method for culturing and further processing of these bacteria. We describe a simple technique for culturing of Mycoplasma hominis and Ureaplasma urealyticum without contamination with other microorganisms and isolating DNA and supernatant. PCR amplification of a chromosomal Mycoplasma fragment was performed as positive control.
Walther
T
T
Max-Delbrueck-Center for Molecular Medicine, Berlin-Buch, Germany.
Retzlaff
C
C
Stepan
H
H
Faber
R
R
D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D019535:Mycoplasma hominis / Q000235:genetics / Q000254:growth & development*; D016133:Polymerase Chain Reaction; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000254:growth & development*; D014621:Vagina / Q000382:microbiology*
D004269:DNA, Bacterial