Immunocytochemical detection of phosphatidylinositol 3 kinase in Burkitt lymphoma cells.
PI 3-kinase, an enzyme responsible for the phosphorylation of the D3 position of the inositol ring of phosphatidylinositol (PI), is recognized to be involved in the regulation of many cellular processes such as mitogenic signalling, inhibition of apoptosis, intracellular vesicle trafficking/secretion, regulation of actin and integrin functions and regulation of protein kinases induced by tumour necrosis factor, oncoproteins and ultraviolet light. Here we report the subcellular distribution and the phosphorylative pattern of p85 alpha subunit of PI 3-kinase in Burkitt lymphoma cells exposed to R interferon alpha treatment. Immunocytochemical analysis of this enzyme, performed by confocal microscopy, revealed an increased expression of this protein at cytoplasmic level after 90 min of interferon alpha treatment. Western blotting analyses performed on nuclear and cytoplasmic fractions confirmed the overexpression found by confocal microscopy at cytoplasmic level in the 90 min interferon alpha treated cells still persisting in the 24 hr treated samples. Such an overexpression was paralleled by an increase of tyrosine phosphorylation both at cytoplasmic and nuclear level suggesting that an enhanced requirement for cytoplasmic expression and phosphorylation of PI 3-kinase might be necessary to the cell for regulating some cytoplasmic-nuclear cross talk involved in the control of Burkitt lymphoma cell metabolism following interferon alpha treatment.
Miscia
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S
Istituto di Morfologia Umana Normale, Università G. D'Annunzio, Chieti, Italy. s.miscia@morpho.unich.it
di Baldassarre
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A
Rana
R A
RA
Pucci
A M
AM
Cataldi
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A
D000818:Animals; D015153:Blotting, Western; D002051:Burkitt Lymphoma / Q000737:chemistry* / Q000201:enzymology* / Q000473:pathology; D002469:Cell Separation; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D019869:Phosphatidylinositol 3-Kinases / Q000032:analysis*; D011817:Rabbits; D014407:Tumor Cells, Cultured
D019869:Phosphatidylinositol 3-Kinases