Viability of eggs, filariform larvae and adults of Strongyloides venezuelensis (Nematoda: Strongyloidea) maintained in vitro.
The present study was performed to check the viability of eggs, filariform larvae and adults of Strongyloides venezuelensis exposed to various conditions for an in vitro maintenance. The eggs in the feces remained viable for about 25 days at 4 degrees C and 15 days at room temperature. However, the isolated eggs in sterile saline lost their viability within 24 hr at 4 degrees C. The eggs in morula stage were very sensitive to air drying and rapidly lost their viability (< or = 12 hr). Filariform larvae survived for a maximum period of 45 days in fecal suspension and 28 days in 0.12% nutrient broth in polyvinyl culture bags maintained at 20 degrees C. On the other hand, those isolated from nutrient broth cultures survived for a maximum period of 32 days in tap water and 22 days in sterile saline at 20 degrees C. The mature adult worms obtained from experimentally infected rats survived maximally for 9 days in serum supplemented (10% rat-serum) 0.12% nutrient broth and 4 days in serum free nutrient broth at 37 degrees C while the culture media were changed at an alternate day. The adult female worms deposited fertile eggs in serum supplemented and serum free nutrient broth cultures, however, the hatched larvae (L1) were not able to develop to the filariform stage in the culture media and found to die within 24 hr of maintenance. The present findings on an in vitro maintenance of different stages of S. venezuelensis may provide useful information for biological and biochemical studies with Strongyloides species.
Baek
B K
BK
Biosafety Research Institute, Chonbuk National University, Chonju, Korea.
Islam
M K
MK
Matsuda
K
K
D000818:Animals; D003470:Culture Media; D005260:Female; D008018:Life Cycle Stages; D051381:Rats; D013321:Strongyloides / Q000196:embryology* / Q000254:growth & development* / Q000648:ultrastructure; D013696:Temperature; D013997:Time Factors; D015053:Zygote / Q000254:growth & development*
D003470:Culture Media