Protein kinase C is involved in cholecystokinin octapeptide-induced proliferative action in rat glioma C6 cells.
Chotecystoknin octapeptide (CCK-8) has been shown to stimulate DNA synthesis in rat glioma C6 cells by activation of CCKB type receptors. However, the signalling pathways contributing to this proliferative action in C6 cells have not been investigated thus far. This study demonstrated that stimulation of rat glioma C6 cells with CCK-8S resulted in activation of protein kinase C isozymes betaI, betaII, gamma and zeta. The participation of protein kinase C in the CCK-8S-induced effect on C6 cell growth was demonstrated by measurement of [3H]thymidine incorporation and estimation of cell number. The data indicate that CCK-8S stimulates growth in rat glioma C6 cells by a protein kinase C-dependent mechanism.
Kaufmann
R
R
Research Unit, Pharmacological Haemostaseology, Medical Faculty at the Friedrich-Schiller-University Jena, Germany. rol@leech.mpg.uni-jena.de
Schafberg
H
H
Zieger
M
M
Henklein
P
P
Nowak
G
G
D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D005910:Glioma; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D064546:Protein Kinase C beta; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*; D012844:Sincalide / Q000494:pharmacology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism / Q000494:pharmacology; D014316:Tritium; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000201:enzymology; D000099221:Protein Kinase C zeta
D002273:Carcinogens; D007527:Isoenzymes; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D012844:Sincalide; D013755:Tetradecanoylphorbol Acetate; D013936:Thymidine; D014316:Tritium; D000099221:Protein Kinase C zeta; C087857:protein kinase C gamma