Formation of reactive oxygen intermediates might be involved in the trypanocidal activity of gallic acid.
We investigated the mechanism of the trypanocidal activity of gallic acid (GA). GA-induced trypanocidal activity was significantly reduced by pretreatment with superoxide dismutase (SOD) and/or catalase. The ESR technique with 5,5-dimethyl-1-pyrroline N-oxide (DMPO) as a spin trapping agent revealed that a DMPO-OH adduct was detected in culture medium containing GA. The intensity of ESR signals of the DMPO-OH adduct was increased in a time dependent manner. SOD also inhibited the formation of GA-induced DMPO-OH adducts. Furthermore, GA enhanced DNA single-strand breaks induced by Fenton reagent. These results suggest the possibility that GA acts as pro-oxidant for trypanocidal activity.
Nose
M
M
Department of Pharmacognosy and Plant Chemistry, Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
Koide
T
T
Morikawa
K
K
Inoue
M
M
Ogihara
Y
Y
Yabu
Y
Y
Ohta
N
N
D000818:Animals; D002374:Catalase / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D003497:Cyclic N-Oxides; D004357:Drug Synergism; D004578:Electron Spin Resonance Spectroscopy; D005707:Gallic Acid / Q000097:blood / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology; D051379:Mice; D016877:Oxidants / Q000097:blood / Q000494:pharmacology*; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013113:Spin Labels; D013482:Superoxide Dismutase / Q000097:blood; D014344:Trypanocidal Agents / Q000097:blood / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
D003497:Cyclic N-Oxides; D016877:Oxidants; D017382:Reactive Oxygen Species; D013113:Spin Labels; D014344:Trypanocidal Agents; D005707:Gallic Acid; C017245:5,5-dimethyl-1-pyrroline-1-oxide; D002374:Catalase; D013482:Superoxide Dismutase; D005990:Glycerol