Detection of Epstein-Barr virus infection in the epithelial cells and lymphocytes of non-neoplastic tonsils by in situ hybridization and in situ PCR.
Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent membrane protein 1 (LMP1)-positive cells were also dispersed in the extrafollicular and germinal center. Using in situ DNA-DNA hybridization, the EBV-positive signals were observed in the upper epithelial cell layers of the tonsils. In addition, in situ PCR detected EBV DNA-positive cells in the lower epithelial cell layers and lymphoid cells.
Kobayashi
R
R
Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.
Takeuchi
H
H
Sasaki
M
M
Hasegawa
M
M
Hirai
K
K
D002908:Chronic Disease; D004279:DNA, Viral / Q000032:analysis; D004847:Epithelial Cells / Q000821:virology; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D014066:Palatine Tonsil / Q000821:virology*; D010610:Pharyngeal Neoplasms; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D014069:Tonsillitis / Q000821:virology
D004279:DNA, Viral; D012367:RNA, Viral