Establishment of a human fibroblast cell line producing tumor necrosis factor alpha (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture.
To develop a new gene therapy model for cancer, a clonal cell line (KMST-6/TNF) which produces human tumor necrosis factor alpha (hTNF-alpha) has been developed by introducing hTNF-alpha cDNA into a human immortal fibroblast cell line (KMST-6). The conditioned medium (CM) of KMST-6/TNF cells inhibited the growth of various malignant human cell lines, but not that of normal human fibroblasts. Although the growth inhibitory effects of KMST-6/TNF CM were neutralized to a considerable degree by anti-TNF-alpha antibody, its inhibitory effects were more marked than the purified human natural TNF-alpha itself in the same units, suggesting that KMST-6/TNF CM contains some growth inhibitory substances other than TNF-alpha. However, interferons alpha, beta, and gamma were undetectable in the KMST-6/TNF CM.
Fushimi
K
K
Department of Cell Biology, Okayama University Medical School, Japan.
Torigoe
K
K
Yamauchi
H
H
Furusako
S
S
Kurimoto
M
M
Namba
M
M
D015152:Blotting, Northern; D002460:Cell Line; D002461:Cell Line, Transformed; D017077:Culture Media, Conditioned; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006131:Growth Inhibitors / Q000378:metabolism*; D006801:Humans; D008297:Male; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
D017077:Culture Media, Conditioned; D006131:Growth Inhibitors; D014409:Tumor Necrosis Factor-alpha