title: Expression of substrate-specific transporters encoded by Plasmodium falciparum in Xenopus laevis oocytes.
abstract: When the malarial parasite Plasmodium falciparum multiplies in erythrocytes it dramatically increases uptake of essential metabolic precursors (nucleosides, nucleobases and glucose) and export of lactic acid by undefined mechanisms. The first evidence is provided here, by a detailed study in Xenopus laevis oocytes, that several specific nutrient transporters are the product of P. falciparum genes. We report the expression of nucleoside, nucleobase, hexose and monocarboxylate transport systems in Xenopus oocytes when injected with mRNA isolated from asexual stages of developing P. falciparum parasites. Their properties are distinct from transport events occurring at the infected erythrocyte membrane or the electrophysiologically identified channel localised to the parasitophorous vacuolar membrane. These novel transporters are substrate-specific and stereoselective, and represent a key regulatory step in the acquisition and export of metabolites by intraerythrocytic P. falciparum.
authors.affiliation: Department of Cellular and Molecular Sciences, St. George's Hospital Medical School, London, UK.
authors.forename: J I
authors.initials: JI
authors.lastname: Penny
authors.forename: S T
authors.initials: ST
authors.lastname: Hall
authors.forename: C J
authors.initials: CJ
authors.lastname: Woodrow
authors.forename: G M
authors.initials: GM
authors.lastname: Cowan
authors.forename: A M
authors.initials: AM
authors.lastname: Gero
authors.forename: S
authors.initials: S
authors.lastname: Krishna
mesh_terms: D000241:Adenosine / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002352:Carrier Proteins / Q000096:biosynthesis*; D003847:Deoxyglucose / Q000378:metabolism; D004912:Erythrocytes / Q000469:parasitology; D015870:Gene Expression; D019271:Hypoxanthine / Q000378:metabolism; D008954:Models, Biological; D009865:Oocytes; D010539:Permeability; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D016053:RNA, Protozoan / Q000235:genetics; D013379:Substrate Specificity; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D019271:Hypoxanthine; D003847:Deoxyglucose; D000241:Adenosine