Anticholinesterase activity of the fluorescent zoanthid pigment, parazoanthoxanthin A.
A synthetic linear tetrazacyclopent(f)azulene compound, parazoanthoxanthin A (m.w. 214.2), strongly fluorescent pigment occurring in zoanthids, was characterized and assayed for anticholinesterase activity. The pigment, emitting fluorescence at lambda(em) 420 nm, was found to be a pure competitive inhibitor of cholinesterases. At pH 8.0, a Ki value of 19 and 26 microM was determined with insect recombinant, and electric eel acetylcholinesterase. Horse serum butyrylcholinesterase was less sensitive with a Ki of 70 microM.
Sepcić
K
K
Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia.
Turk
T
T
Macek
P
P
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D002800:Cholinesterase Inhibitors / Q000032:analysis*; D003063:Cnidaria / Q000737:chemistry*; D003508:Cycloheptanes / Q000737:chemistry*; D008387:Marine Toxins / Q000032:analysis*
D002800:Cholinesterase Inhibitors; D003508:Cycloheptanes; D008387:Marine Toxins; C114237:parazoanthoxanthin A; D000110:Acetylcholinesterase