Cellular distribution of 111In-LDTPA galactose BSA in normal and asialoglycoprotein receptor-deficient mouse liver.
111In-LDTPA galactose BSA (bovine serum albumin) was used to evaluate the asialoglycoprotein receptor (ASGPR) system in both normal and ASGPR-deficient mice. The radiolabeled glycoprotein had complete liver uptake in both normal and ASGPR-deficient mice. Metabolism and hepatic cell-type distribution studies were performed. The normal mouse excreted greater than 60% of the hepatic activity, while the ASGPR-deficient mouse excreted less than 40% of the hepatic activity. 111In-LDTPA galactose BSA was metabolized to 111In-LDTPA-L-lysine in both mouse types. Normal mice showed 70% of the radioactivity in the hepatocyte, whereas the homozygous ASGPR-deficient mouse had equal activity in the hepatocyte and the hepatic endothelial cell.
Deal
K A
KA
Mallinckrodt Institute of Radiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Cristel
M E
ME
Welch
M J
MJ
D000818:Animals; D000931:Antidotes / Q000378:metabolism* / Q000493:pharmacokinetics; D037263:Asialoglycoprotein Receptor; D002478:Cells, Cultured; D005260:Female; D005690:Galactose / Q000378:metabolism* / Q000493:pharmacokinetics; D007205:Indium Radioisotopes; D008099:Liver / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D051379:Mice; D008810:Mice, Inbred C57BL; D004369:Pentetic Acid / Q000031:analogs & derivatives / Q000378:metabolism* / Q000493:pharmacokinetics; D011956:Receptors, Cell Surface / Q000172:deficiency*; D014018:Tissue Distribution
D000931:Antidotes; D037263:Asialoglycoprotein Receptor; D007205:Indium Radioisotopes; D011956:Receptors, Cell Surface; D004369:Pentetic Acid; D005690:Galactose