Salicylic acid induces a cytosolic Ca2+ elevation in yeast.
Cytosolic free calcium ion concentration ([Ca2+]cyt) after a salicylic acid (SA)-stimulus was monitored in cells of the yeast Saccharomyces cerevisiae expressing apoaequorin, which constitutes a Ca(2+)-sensitive luminescent protein, aequorin, when combined with coelenterazine. SA induced a transient [Ca2+]cyt elevation that was dependent on the concentration of SA and pH of the SA solution. The SA-induced [Ca2+]cyt elevation was not reduced in Ca(2+)-deficient medium, suggesting that Ca2+ was mobilized from an intracellular Ca2+ store(s). Benzoic acid, butyric acid and sorbic acid did not induced a [Ca2+]cyt elevation.
Mori
I C
IC
Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Iida
H
H
Tsuji
F I
FI
Isobe
M
M
Uozumi
N
N
Muto
S
S
D000331:Aequorin / Q000378:metabolism*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D008163:Luminescent Measurements; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid
D000894:Anti-Inflammatory Agents, Non-Steroidal; D010937:Plant Growth Regulators; D012459:Salicylates; D000331:Aequorin; D020156:Salicylic Acid; D002118:Calcium