Identification of linear DNA plasmids of the yeast Pichia pastoris.
Two DNA plasmids, approximately 11 and 8 kb in size, have been identified in a strain of the yeast Pichia pastoris (Northern Regional Research Laboratories No. Y4290). The plasmids are resistant to RNase A and lambda exonuclease, but are sensitive to digestion by DNase I, suggesting that they are linear and double-stranded DNA with 5'-protected ends. A restriction map has been constructed for the 11-kb plasmid, confirming that it is linear.
Banerjee
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Department of Biology, Long Island University, Brooklyn Campus, New York, USA.
Kopvak
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Curley
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D
D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D003850:Deoxyribonuclease I / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005092:Exonucleases / Q000737:chemistry; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification*; D015183:Restriction Mapping; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
D004269:DNA, Bacterial; D005092:Exonucleases; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic