A functional eukaryotic promoter is contained within the first intron of the hGH-N coding region.
Human growth hormone is frequently used as a reporter gene in studies addressing the regulation of eukaryotic promoters. Here we present evidence that the first intron of the hGH-N coding region contains a novel eukaryotic promoter which is able to direct the expression of hGH-N and luciferase reporter genes. The corresponding transcriptional initiation site is located downstream of the splice acceptor site of exon 2. This internal hGH-N promoter can interfere with the transcriptional control elements of a promoter linked to the hGH-N coding region and thereby complicate the interpretation of data obtained with hGH-N as a reporter gene.
Kolb
A F
AF
Institute of Virology and Immunology, University of Würzburg, Germany. kolba@main.hri.sari.ac.uk
Günzburg
W H
WH
Brem
G
G
Erfle
V
V
Salmons
B
B
D000818:Animals; D001483:Base Sequence; D002415:Cats; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D017930:Genes, Reporter; D019382:Human Growth Hormone / Q000235:genetics*; D006801:Humans; D007438:Introns; D008156:Luciferases / Q000235:genetics; D011401:Promoter Regions, Genetic; D014162:Transfection
D017931:DNA Primers; D019382:Human Growth Hormone; D008156:Luciferases