title: Structural analysis of the exopolysaccharides produced by Lactobacillus spp. G-77.
abstract: The exopolysaccharide produced by a ropy strain of Lactobacillus spp. G-77 in a semi-defined medium, was found to be a mixture of two homopolymers composed of D-Glc. The two poly-saccharides were separated and, on the basis of monosaccharide and methylation analyses, 1H, 13C, 1D and 2D NMR experiments, one of the polysaccharides was shown to be a 2-substituted-(1-3)-beta-D-glucan, identical to that described for the EPS from Pediococcus damnosus 2.6 (M.T. Dueñas-Chasco, M.A. Rodríguez-Carvajal, P. Tejero-Mateo, G. Franco-Rodríguez, J. L. Espartero, A. Irastorza-Iribar, and A.M. Gil-Serrano, Carbohydr. Res., 303 (1997) 453-458), and the other polysaccharide was shown to consist of repeating units with the following structure [formula: see text]
authors.affiliation: Departamento de Química Aplicada, Facultad de Ciencias Químicas, Universidad del Pais Vasco, San Sebastian, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: Dueñas-Chasco
authors.forename: M A
authors.initials: MA
authors.lastname: Rodríguez-Carvajal
authors.forename: P
authors.initials: P
authors.lastname: Tejero-Mateo
authors.forename: J L
authors.initials: JL
authors.lastname: Espartero
authors.forename: A
authors.initials: A
authors.lastname: Irastorza-Iribas
authors.forename: A M
authors.initials: AM
authors.lastname: Gil-Serrano
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D007778:Lactobacillus / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010376:Pediococcus / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D013237:Stereoisomerism
chemical_list: D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial