title: Quantitative immunoelectron microscopy reveals alpha2,6 sialyltransferase is concentrated in the central cisternae of rat hepatocyte Golgi apparatus.
abstract: The Golgi apparatus is a membrane bound organelle involved in synthesis of N-linked oligosaccharides which are trimmed and then lengthened by a series of sugar transferases adding N-acetylglucosamine, galactose and sialic acid in sequence. We previously published qualitative work which localized Galbeta1,4GlcNAc alpha2,6 sialyltransferase of rat hepatocytes to the trans cisternae and the trans Golgi network. We now report the use of combined stereological and immunoelectron microscopical techniques for mapping the Golgi stack composition and distribution of sialyltransferase protein in rat hepatocytes. The Golgi stack showed substantial variation in composition consisting of 1, 2, 3, 4, or 5 cisternae with an average of 2.5 cisternae. Sialyltransferase labeling was mainly located in the central cisternae of the Golgi stacks irrespective of whether the stacks were oriented in a cis/trans direction using morphological criteria. Only 20% of the total sialyltransferase labeling was present in the transmost cisterna and 2% in the trans Golgi Network. The low labeling in the transmost cisterna was essentially due to the presence of a sialyltransferase negative cisterna. These data emphasize the importance of quantitation in obtaining a representative picture of Golgi enzyme distribution in three dimensions. They indicate that central cisternae, rather than the transmost cisterna and TGN, function in sialylation along the secretory pathway of rat hepatocytes.
authors.affiliation: Department of Anatomy and Physiology, Medical Sciences Institute, University of Dundee, Scotland.
authors.forename: C
authors.initials: C
authors.lastname: Lovelock
authors.forename: J
authors.initials: J
authors.lastname: Lucocq
mesh_terms: D000818:Animals; D006056:Golgi Apparatus / Q000201:enzymology* / Q000648:ultrastructure; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D016253:Microscopy, Immunoelectron; D051381:Rats; D017208:Rats, Wistar; D012799:Sialyltransferases / Q000032:analysis*; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D012799:Sialyltransferases; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase