title: Fas antigen expression and apoptosis of lymphocytes in macaques infected with simian immunodeficiency virus strain mac.
abstract: To investigate the role of apoptosis in the pathogenesis of HIV infection we used macaques infected with simian immunodeficiency virus (SIV) as a primate model and examined the characteristics of the apoptosis of lymphocytes in SIV mac-infected macaques. In vitro apoptosis was more strongly induced in peripheral blood mononuclear cells (PBMC) from SIV mac239-infected macaques than those from uninfected controls. We found that the frequency of Fas antigen-positive cells was higher in PBMC from SIV mac-infected macaques than from uninfected controls, and in vitro apoptosis of PBMC was suppressed by an inhibitor of the interleukin-1 beta converting enzyme (ICE) family proteases. In biopsied lymph nodes, the number of apoptotic nuclei in T cell-dependent areas was higher in SIV mac-infected macaques than in uninfected controls. A higher number of apoptotic nuclei in lymph nodes of SIV mac-infected macaques was observed in the stage of persistent general lymphadenopathy than in those with AIDS-related complex, while there was no significant difference in the extent of apoptosis of cultured PBMC among the SIV mac-infected macaques. These results suggest that in vitro apoptosis is mediated by the Fas/Fas ligand and ICE system and that apoptosis in lymph nodes may be more closely related to the stage of SIV mac infection than is that of cultured PBMC.
authors.affiliation: Department of Microbiology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Igarashi
authors.forename: H
authors.initials: H
authors.lastname: Ichimura
authors.forename: T
authors.initials: T
authors.lastname: Kuwata
authors.forename: T
authors.initials: T
authors.lastname: Shimada
authors.forename: D
authors.initials: D
authors.lastname: Nagamachi
authors.forename: S
authors.initials: S
authors.lastname: Yonehara
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
authors.forename: M
authors.initials: M
authors.lastname: Hayami
mesh_terms: D000818:Animals; D017209:Apoptosis; D020170:Caspase 1; D002478:Cells, Cultured; D003546:Cysteine Endopeptidases / Q000378:metabolism; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004195:Disease Models, Animal; D018450:Disease Progression; D015658:HIV Infections / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D008198:Lymph Nodes / Q000473:pathology; D008251:Macaca; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology*; D019014:fas Receptor / Q000096:biosynthesis*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D019014:fas Receptor; D003546:Cysteine Endopeptidases; D020170:Caspase 1