A putative human zinc-finger gene (ZFPL1) on 11q13, highly conserved in the mouse and expressed in exocrine pancreas. The European Consortium on MEN 1.
In the process of identification of the multiple endocrine neoplasia type 1 gene, which was recently published, we isolated a novel gene in the 11q13 region. This gene (named ZFPL1, for zinc-finger protein-like 1) is expressed strongly in the exocrine pancreas as a 1.4-kb polyadenylated RNA encoding a putative protein of 310 amino acids. A mouse EST contig predicts an equally sized murine protein with 91% amino acid sequence identity to the human protein. No significant homology with known proteins could be found through database screening. However, zinc-finger-like domains and leucine-zipper-like motifs in the predicted ZFPL1 protein were identified, suggesting the presence of DNA-binding and dimerization domains possibly involved in transcription regulation. This notion is supported by the presence of a putative bipartite nuclear localization signal. This paper presents the full-length cDNA sequence for this gene, its genomic structure and chromosomal orientation, and expression studies by Northern blot hybridization and RNA in situ hybridization.
Höppener
J W
JW
Department of Internal Medicine, Utrecht University Hospital, The Netherlands. j.w.m.hoeppener@lab.azu.nl
De Wit
M J
MJ
Simarro-Doorten
A Y
AY
Roijers
J F
JF
van Herrewaarden
H M
HM
Lips
C J
CJ
Parente
F
F
Quincey
D
D
Gaudray
P
P
Khodaei
S
S
Weber
G
G
Teh
B
B
Farnebo
F
F
Larsson
C
C
Zhang
C X
CX
Calender
A
A
Pannett
A A
AA
Forbes
S A
SA
Bassett
J H
JH
Thakker
R V
RV
Lemmens
I
I
Van de Ven
W J
WJ
Kas
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns / Q000235:genetics; D016350:Leucine Zippers; D051379:Mice; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017422:Sequence Analysis, DNA; D016335:Zinc Fingers / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C114477:ZFPL1 protein, human; D012313:RNA