Cellular specificity of fibroblastic proMMP-3 downregulation by normal or cancer trophoblastic cells.
It has been shown previously that endometrial fibroblast prometalloproteinase-3 (proMMP-3) secretion is inhibited by normal cytotrophoblasts as a result of paracrine interactions. To investigate the specificity of this downregulation, two different types of co-culture were used. First, peritoneal and synovial fibroblasts obtained from human tissue biopsies were co-cultured with normal cytotrophoblasts. By contrast to what has been observed with endometrial fibroblasts, their proMMP-3 secretion increased though their morphology was slightly modified. Secondly, the regulation of endometrial proMMP-3 secretion was tested in co-cultures with two choriocarcinoma cell lines (JAr and JEG-3 cell lines). With tumoral cytotrophoblastic cells, endometrial fibroblasts became decidua-like cells and JAr and JEG-3 cell lines grew in syncytium. However, co-culture of endometrial fibroblasts with JAr and JEG-3 choriocarcinoma cells did not decrease proMMP-3 secretion. The present results show that endometrial proMMP-3 downregulation is tissue specific and does not depend on cell differentiation. A cytotrophoblast cell membrane factor which might not be expressed in tumoral cells could probably play a role in paracrine relations between epithelial cells and surrounding stroma.
Bellingard-Dubouchaud
V
V
INSERM U 148, Hormones et Cancer, Montpellier, France.
Hédon
B
B
Maudelonde
T
T
D002822:Choriocarcinoma / Q000201:enzymology*; D018920:Coculture Techniques; D015536:Down-Regulation; D004717:Endometrium / Q000201:enzymology*; D004792:Enzyme Precursors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009928:Organ Specificity; D010537:Peritoneum / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D013583:Synovial Membrane / Q000166:cytology / Q000378:metabolism; D014327:Trophoblasts / Q000201:enzymology*; D014407:Tumor Cells, Cultured; D014594:Uterine Neoplasms / Q000201:enzymology*
D004792:Enzyme Precursors; D008666:Metalloendopeptidases; C048047:prostromelysin