title: Bromodeoxyuridine incorporation and Ki 67 expression in oral hairy leukoplakia.
abstract: OBJECTIVE
  Oral hairy leukoplakia (HL) is an acanthotic, hyperparakeratotic lesion characterised by the presence of a replicative Epstein-Barr virus (EBV) infection in the superficial and adjoining layers of the epithelium. EBV or its gene products are capable of modifying epithelial differentiation. The aim of this study was to establish whether the presence of EBV was associated with an alteration in cell turnover by assessing bromodeoxyuridine (BrdU) incorporation and Ki 67 expression in lesional tissue and control mucosa.
  
  
  METHODS
  Biopsies of HL together with age, site and sex matched controls (n = 7 and 8 respectively) were incubated in 200 microM BrdU in vitro, fixed in methacarn and processed to paraffin wax. Following acid hydrolysis, incorporated BrdU and Ki 67 were identified in serial 5 microns sections using a three-stage immunoperoxidase technique and cell density expressed as the number of positive cells per mm basement membrane length.
  
  
  RESULTS
  Overall, there was no difference in the number of BrdU positive cells per mm basement membrane length between control and HL tissue. However, within HL alone, the presence of focal EBV replication was associated with a significant reduction in the number of basal cells incorporating BrdU compared to adjacent EBV free areas (P < 0.05). There was no significant difference between Ki 67 positive cells in control and HL tissue and no evidence of a reduction of Ki 67 positive cells in areas associated with EBV replication.
  
  
  CONCLUSIONS
  These results suggest that there is no evidence of a generalised alteration of the proliferative capacity of basal cells in HL, although the focal reduction in BrdU incorporation may reflect subtle changes on cell turnover by EBV infection.
authors.affiliation: Oral Diseases Research Centre, St Bartholomew's, London, UK. a.t.cruchley@mds.qmw.ac.uk
authors.forename: A T
authors.initials: AT
authors.lastname: Cruchley
authors.forename: Y G
authors.initials: YG
authors.lastname: De Souza
authors.forename: D M
authors.initials: DM
authors.lastname: Williams
authors.forename: D
authors.initials: D
authors.lastname: Greenspan
authors.forename: J S
authors.initials: JS
authors.lastname: Greenspan
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000378:metabolism; D016022:Case-Control Studies; D002455:Cell Division; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000502:physiology; D019394:Ki-67 Antigen / Q000032:analysis / Q000096:biosynthesis*; D017733:Leukoplakia, Hairy / Q000378:metabolism / Q000503:physiopathology* / Q000821:virology; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000378:metabolism; D014779:Virus Replication
chemical_list: D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D001973:Bromodeoxyuridine