Metabolism of explosive compounds by sulfate-reducing bacteria.
The metabolism of various explosive compounds-1,3,5-trinitrobenzene (TNB), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetraazocine (HMX)-by a sulfate-reducing bacterial consortium, Desulfovibrio spp., was studied. The results indicated that the Desulfovibrio spp. used all of the explosive compounds studied as their sole source of nitrogen for growth. The concentrations of TNB, RDX, and HMX in the culture media dropped to below the detection limit (<0.5 ppm) within 18 days of incubation. We also observed the production of ammonia from the nitro groups of the explosive compounds in the culture media. This ammonia served as a nitrogen source for the bacterial growth, and the concentration of ammonia later dropped to <0.5 mg/L. The sulfate-reducing bacteria may be useful in the anaerobic treatment of explosives-contaminated soil.
Boopathy
R
R
Environmental Research Division, Argonne National Laboratory, Argonne, IL 60439, USA.
Gurgas
M
M
Ullian
J
J
Manning
J F
JF
D000641:Ammonia / Q000378:metabolism; D001392:Azocines / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003901:Desulfovibrio / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D015386:Hazardous Substances / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D006573:Heterocyclic Compounds, 1-Ring / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D012378:Rodenticides / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D016999:Sulfur-Reducing Bacteria / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D014227:Triazines / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D014301:Trinitrobenzenes / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D014303:Trinitrotoluene / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology
D001392:Azocines; D015386:Hazardous Substances; D006573:Heterocyclic Compounds, 1-Ring; D012378:Rodenticides; D013431:Sulfates; D014227:Triazines; D014301:Trinitrobenzenes; D014303:Trinitrotoluene; D000641:Ammonia; C007950:octogen; C009160:cyclonite