Adhesive characteristics of type II pneumocyte subpopulations from saline- and silica-treated rats.
Alveolar epithelial cells isolated from silica-treated rat lungs provide a system for the in vitro study of repair mechanisms. In studies of type II cell interactions with the extracellular matrix, we observed that type IIB pneumocytes from silica-treated rats adhered to tissue culture plastic more readily than do normal type II cells. This paper examines the adhesion characteristics of IIA and IIB cells and their modulation by divalent cations. We describe differences in the adhesive behavior of two subpopulations of freshly isolated type II pneumocytes from saline- and silica-treated rats. The observations have implications for repair and tissue remodeling in the lung.
Sigurdson
S L
SL
Department of Internal Medicine, SUNY, Buffalo School of Medicine, Buffalo General Hospital 14203, USA.
Lwebuga-Mukasa
J S
JS
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D016326:Extracellular Matrix Proteins / Q000187:drug effects / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D016023:Integrins / Q000378:metabolism; D008297:Male; D011650:Pulmonary Alveoli / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012822:Silicon Dioxide / Q000633:toxicity; D012829:Silicosis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D012965:Sodium Chloride / Q000633:toxicity
D002413:Cations, Divalent; D016326:Extracellular Matrix Proteins; D016023:Integrins; D012965:Sodium Chloride; D012822:Silicon Dioxide