Soluble guanylate cyclase in Saccharomyces cerevisiae.
After removing nonspecific immunoreactivities from crude extract by immunoaffinity chromatography, an immunoreactive-band at 40kDa of soluble guanylate cyclase (SGC) from Saccharomyces cerevisiae was detected by Western blot using rabbit anti-beta 1 subunit of SGC. Cyclic GMP level and SGC activity was measured by ELISA. Immunoprecipitated yeast SGC was activated by sodium nitroprusside, whereas inhibited by 1H-(1,2,4)oxadiazolo(4,3-A)quinoxalin-1-one. Increased cyclic GMP level was also noted when intact yeast cells were incubated with s-nitrosoglutathione, a NO donor. The result implies that NO can be utilized intracellularly and extracellularly. Moreover, the presence of SGC suggests the significance of NO/cyclic GMP signaling in unicellular eukaryotes.
Kuo
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WN
Division of Science and Math, Bethune-Cookman College, Daytona Beach, Florida 32115, USA.
Kanadia
R N
RN
Mcnabb
M
M
D006152:Cyclic GMP / Q000378:metabolism; D004795:Enzyme Stability; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology; D010069:Oxadiazoles / Q000494:pharmacology; D011233:Precipitin Tests; D011810:Quinoxalines / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012995:Solubility
C095284:1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; D010069:Oxadiazoles; D011810:Quinoxalines; D009599:Nitroprusside; D006162:Guanylate Cyclase; D006152:Cyclic GMP