title: Efficacy of inactivated H5N2 influenza vaccines against lethal A/Chicken/Queretaro/19/95 infection.
abstract: The control and eventual eradication of H5N2 influenza virus from domestic poultry in Mexico is dependent on the use of avian influenza (AI) vaccine strategies. This study was performed to determine the amount of hemagglutinin (HA) antigen required to control the signs of disease from a highly pathogenic H5N2 influenza virus (A/Chicken/ Queretaro/19/95) and the amount of antigen required to prevent shedding of virus from vaccinated birds. Six commercial inactivated water in oil H5N2 vaccines available in Mexico were compared with standardized vaccines to assess their efficacy. The amount of HA required to prevent the signs of disease from A/Chicken/Queretaro/19/95 influenza virus was approximately 0.4 microgram per dose. Each of the six commercially available vaccines prevented disease signs, and half of the vaccines significantly reduced viral shedding from vaccinated birds. There is a need for standardization of AI virus vaccine, and the antigen content should be increased in some of the commercially available AI vaccines in Mexico.
authors.affiliation: Department of Virology and Molecular Biology, St. Jude Children's Research Hospital Memphis, TN 38105, USA.
authors.forename: A
authors.initials: A
authors.lastname: Garcia
authors.forename: H
authors.initials: H
authors.lastname: Johnson
authors.forename: D K
authors.initials: DK
authors.lastname: Srivastava
authors.forename: D A
authors.initials: DA
authors.lastname: Jayawardene
authors.forename: D R
authors.initials: DR
authors.lastname: Wehr
authors.forename: R G
authors.initials: RG
authors.lastname: Webster
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002645:Chickens; D002988:Cloaca / Q000821:virology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D053125:Influenza A Virus, H5N2 Subtype; D009980:Influenza A virus / Q000276:immunology*; D007252:Influenza Vaccines / Q000276:immunology / Q000592:standards*; D005585:Influenza in Birds / Q000517:prevention & control*; D013047:Specific Pathogen-Free Organisms; D014132:Trachea / Q000821:virology; D015164:Vaccines, Inactivated / Q000276:immunology / Q000592:standards; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D015164:Vaccines, Inactivated