Design and Application of Two Rapid Screening Techniques for Isolation of Mn(IV) Reduction-Deficient Mutants of Shewanella putrefaciens.
Chemical mutagenesis procedures and two newly developed rapid plate assays were used to identify two Mn(IV) reduction-deficient (Mnr) mutants of Shewanella putrefaciens. All eleven members of a set of previously isolated Fe(III) reduction-deficient (Fer) mutants displayed Mnr-positive phenotypes on the plate assays and were also capable of anaerobic growth on Mn(IV) as the sole terminal electron acceptor.
Burnes
BS
School of Biology, Georgia Institute of Technology, Atlanta, Georgia 30332.
Mulberry
MJ
DiChristina
TJ