Use of generic fast gradient liquid chromatography-tandem mass spectroscopy in quantitative bioanalysis.
Short narrow analytical HPLC columns have been used successfully with high linear flow-rates and combined with mass spectrometric detection to produce a generic approach to quantitative bioanalysis. The approach has been used to validate several assays in the low ng/ml region and an example is given in this paper. When combined with a simple solid-phase extraction process the need for complicated, time consuming method development has been removed for the majority of pharmaceutical compounds. The approach takes advantage of not only the extra selectivity of the MS-MS detector but the excellent resolution and peak shape produced by gradient elution.
Ayrton
J
J
International Development, Bioanalysis and Drug Metabolism Division, GlaxoWellcome Research and Development, Ware, Herts, UK.
Dear
G J
GJ
Leavens
W J
WJ
Mallett
D N
DN
Plumb
R S
RS
D000083:Acetanilides / Q000032:analysis; D000818:Animals; D001774:Blood Chemical Analysis / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003408:Cresols / Q000032:analysis; D007659:Ketones / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013806:Theophylline / Q000032:analysis; D014498:Uracil / Q000032:analysis
D000083:Acetanilides; D003408:Cresols; D007659:Ketones; D004364:Pharmaceutical Preparations; D014498:Uracil; D013806:Theophylline; C042041:3-cresol