title: Purification of a 5-HT uptake inhibitor from the venom of Cerastes vipera.
abstract: A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13,739 Da and an IC50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.
authors.affiliation: Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, UK.
authors.forename: M
authors.initials: M
authors.lastname: Alzahaby
authors.forename: A L
authors.initials: AL
authors.lastname: Harvey
authors.forename: L C
authors.initials: LC
authors.lastname: Young
authors.forename: G
authors.initials: G
authors.lastname: Faure
authors.forename: E G
authors.initials: EG
authors.lastname: Rowan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D017367:Selective Serotonin Reuptake Inhibitors / Q000302:isolation & purification*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014757:Viper Venoms / Q000032:analysis* / Q000494:pharmacology; D017819:Viperidae
chemical_list: D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D014757:Viper Venoms