title: Diagnosis of cerebral toxoplasmosis by a nested polymerase chain reaction: a case report.
abstract: A 24-year-old man infected with human immunodeficiency virus type I rapidly progressed to acquired immune deficiency syndrome. His clinical picture was compatible with a presumptive diagnosis of cerebral toxoplasmosis. Application of a nested polymerase chain reaction (PCR) succeeded in detecting Toxoplasma gondii DNA in both the venous blood and cerebrospinal fluid specimens. This result indicates that PCR is a convenient tool for making a rapid and accurate diagnosis of cerebral toxoplasmosis, especially in developing countries.
authors.affiliation: Department of Neurology, Chushang Show-Chwan Hospital, Nantou, Taipei, Taiwan, ROC.
authors.forename: J J
authors.initials: JJ
authors.lastname: Lin
authors.forename: H J
authors.initials: HJ
authors.lastname: Harn
mesh_terms: D000328:Adult; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D016781:Toxoplasmosis, Cerebral / Q000175:diagnosis*