title: [Estradiol Reference Standard (Control 961) of National Institute of Health Sciences].
abstract: The raw material for estradiol was examined for preparation of the "Estradiol Reference Standard (Control 961)" of National Institute of Health Sciences. Analytical data obtained were as follows: melting point, 179.1 degrees C; UV spectrum, lambda max = 281 nm; IR spectrum, the same as that of the Estradiol Reference Standard of National Institute of Health Sciences (Control 931); optical rotation, [alpha]20D = +79.5 degrees; thin-layer chromatography, one impurity was detected; high-performance liquid chromatography (HPLC), a trace amount of three impurities were detected; loss on drying, 3.17%; assay, 99.4% by UV spectrophotometry and 99.0% by HPLC. Based on the above results, the raw material was authorized as the Estradiol Reference Standard (Control 961) of National Institute of Health Sciences.
authors.forename: A
authors.initials: A
authors.lastname: Kitajima
authors.forename: Y
authors.initials: Y
authors.lastname: Tagashira
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
authors.forename: K
authors.initials: K
authors.lastname: Yoshii
authors.forename: H
authors.initials: H
authors.lastname: Komatsu
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D004958:Estradiol / Q000592:standards*; D007564:Japan; D012015:Reference Standards
chemical_list: D004958:Estradiol