title: Isolation and characterization of sorbitol-fermenting Shiga toxin (Verocytotoxin)-producing Escherichia coli O157:H- strains in the Czech Republic.
abstract: Two sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H- strains were isolated from patients with hemolytic-uremic syndrome in the Czech Republic in 1995. Their phenotypic and genotypic characteristics and genomic DNA fingerprints were identical or closely related to those of SF STEC O157:H- strains isolated in Germany in 1988 to 1997. This indicates that the Czech isolates belong to the SF STEC O157 clone which is widespread in Germany. It is the first finding of the clone outside Germany.
authors.affiliation: Institute of Medical Microbiology, The 2nd Medical Faculty, Charles University, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Bielaszewska
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: M A
authors.initials: MA
authors.lastname: Karmali
authors.forename: R
authors.initials: R
authors.lastname: Khakhria
authors.forename: J
authors.initials: J
authors.lastname: Janda
authors.forename: K
authors.initials: K
authors.lastname: Bláhová
authors.forename: H
authors.initials: H
authors.lastname: Karch
mesh_terms: D001427:Bacterial Toxins / Q000096:biosynthesis*; D018153:Czech Republic; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004927:Escherichia coli Infections / Q000382:microbiology*; D019453:Escherichia coli O157 / Q000145:classification* / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005838:Genotype; D006463:Hemolytic-Uremic Syndrome / Q000382:microbiology*; D006801:Humans; D007223:Infant; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D019105:Random Amplified Polymorphic DNA Technique; D022622:Shiga Toxin 1; D013012:Sorbitol / Q000378:metabolism*
chemical_list: D001427:Bacterial Toxins; D004269:DNA, Bacterial; D022622:Shiga Toxin 1; D013012:Sorbitol