Evaluation of third generation anti-HCV test kit (SYNPEP HCV-EIA II) using sera of inhabitants from HCV hyperendemic area.
We examined the characteristics and usefulness of a third generation anti-HCV test kit, SYNPEP HCV-EIA II (Kyokuto Pharmaceutical Inc., Tokyo, Japan). The sera of inhabitants from a hepatitis C virus (HCV) hyperendemic area were used. The kit had even or more anti-HCV detection sensitivity and reproducibility than ORTHO HCV III ELISA Test System (Ortho-Clinical Diagnostic K.K., Tokyo, Japan) or HCV PHA 2nd Generation (Dinabot Co., Ltd., Tokyo, Japan). SYNPEP HCV-EIA II needed less total reaction time than other EIA kits, resulting in a simple procedure. Also, HCV RNA was detected in 90% of subjects who had a 7.5 or greater cut-off index (COI) of SYNPEP HCV-EIA II kit. In conclusion, SYNPEP HCV-EIA II require cheap cost and simple procedure and it could be applied to mass screening to find out HCV RNA positive persons who may need clinical care.
Shirachi
M
M
Second Department of Medicine, Kurume University School of Medicine, Japan.
Sata
M
M
Suzuki
H
H
Fukuizumi
K
K
Tanikawa
K
K
Itoh
Y
Y
Mizumoto
T
T
Kondoh
S
S
Kasahara
M
M
Manabe
S
S
D000914:Antibodies, Viral / Q000097:blood*; D019353:Endemic Diseases; D005069:Evaluation Studies as Topic; D016174:Hepacivirus / Q000276:immunology / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000453:epidemiology; D006801:Humans; D007124:Immunoenzyme Techniques; D007564:Japan / Q000453:epidemiology; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic
D000914:Antibodies, Viral; D011933:Reagent Kits, Diagnostic