Determination of free concentration of sameridine in blood plasma by ultrafiltration and coupled-column liquid chromatography.
Sameridine is a new candidate drug with both local anaesthetic and analgesic properties. The free concentration of sameridine in blood plasma was determined by coupled-column liquid chromatography. Following adjustment of the pH and the temperature of the plasma samples, the free fraction was prepared by ultrafiltration. The coupled-column liquid chromatographic system consisted of a reversed-phase column, a cation-exchange extraction column and a cation-exchange analytical column. Sameridine was detected by UV determination at 205 nm and the system showed high selectivity. The limit of quantification was 1 nM and the within-day precision was 4.6% (R.S.D., n = 10).
Eklund
E
E
Concept Division, Bioanalytical Chemistry, Astra Pain Control AB, Södertälje, Sweden.
Norsten-Höög
C
C
Arvidsson
T
T
D000700:Analgesics / Q000097:blood*; D000779:Anesthetics, Local / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D010880:Piperidines / Q000097:blood*; D011506:Proteins / Q000378:metabolism; D014462:Ultrafiltration
D000700:Analgesics; D000779:Anesthetics, Local; D010880:Piperidines; D011506:Proteins; C113809:sameridine