Principles and microscopic confirmation of surface quality of two new waterjet-based microkeratomes.
OBJECTIVE
The HydroBlade and the HydroBrush keratomes are waterjet-based devices for corneal surgery that operate at normal intraocular pressure in two different modes: removal of parallel or shaped lenticules or hinged flaps with a small diameter, high speed waterjet; and removal of the epithelium with a waterjet sheet. The operating principles as well as histology of the cut surfaces are described.


METHODS
A flap was made in one cadaver eye with a Chiron ACS keratome and in the second eye with the HydroBlade keratome. The epithelium was removed in one cadaver eye with a surgical blade and in the second eye by the HydroBrush keratome. Scanning and transmission electron microscopy and light microscopy was obtained.


RESULTS
The HydroBlade keratome cleaved only cross-linking fibrils and left intact keratocytes. Shape and dimensions of the flap were accurate. There was no observable hydration or significant heating of the tissue. Mechanical forces on the cornea were small. The HydroBrush keratome removed the epithelium quickly, left no epithelial debris, and did not damage Bowman's layer.


CONCLUSIONS
With the HydroBlade keratome, the cuts are ideal blunt dissections. Epithelial removal with the HydroBrush keratome is effective and quick.
Gordon
E
E
Medjet Inc, Edison, NJ 08837, USA.
Parolini
B
B
Abelson
M
M
D003315:Cornea / Q000601:surgery* / Q000648:ultrastructure*; D019573:Epithelium, Corneal / Q000648:ultrastructure; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013508:Ophthalmologic Surgical Procedures / Q000295:instrumentation*