title: Requirements for B7-CD28 costimulation in mucosal IgA responses: paradoxes observed in CTLA4-H gamma 1 transgenic mice.
abstract: The block in the CD80/CD86-CD28/CTLA-4 pathway in CTLA4-H gamma 1 transgenic (Tg) mice results in strongly impaired systemic IgG immunity and failure to develop germinal center reactions. By contrast, here we report that mucosal immunity and IgA B cell differentiation are not affected by this block. We found abundant germinal centers and evidence of IgA switch differentiation in Peyer's patches, normal total IgA levels, and normal numbers of IgA-labeling cells in the gut mucosa. The distribution of B-1 and B-2 cells and the relative contribution of B-1 cells to the total IgA B cells were similar in Tg and wild-type mice. Despite this, oral immunizations with keyhole limpet hemocyanin plus cholera toxin adjuvant failed to stimulate Ag-specific mucosal IgA responses in CTLA4-H gamma 1 Tg mice. This was not due to a lack of adjuvant activity of cholera toxin in Tg mice, nor was this secondary to an inability to take up Ag from the gut lumen. Rather, CD4+ T cells stimulated by oral immunization in Tg mice appeared to be inappropriately primed, as evidenced by a significantly reduced level of CD40 ligand and CD44 expression and an increased expression of CD95 compared to those in wild-type mice. This study reveals a paradox in the regulation of mucosal IgA responses.
authors.affiliation: Department of Medical Microbiology and Immunology, University of Goteborg, Sweden.
authors.forename: E
authors.initials: E
authors.lastname: Gärdby
authors.forename: P
authors.initials: P
authors.lastname: Lane
authors.forename: N Y
authors.initials: NY
authors.lastname: Lycke
mesh_terms: D000069594:Abatacept; D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000235:genetics*; D016175:B-Lymphocyte Subsets / Q000276:immunology; D018122:B7-1 Antigen / Q000502:physiology*; D018106:CD28 Antigens / Q000502:physiology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D060908:CTLA-4 Antigen; D002772:Cholera Toxin / Q000276:immunology; D005260:Female; D005751:Gastric Lavage; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D006801:Humans; D007114:Immunization; D018796:Immunoconjugates; D007070:Immunoglobulin A / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D000943:Antigens, Differentiation; D018122:B7-1 Antigen; D018106:CD28 Antigens; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556707:Ctla4 protein, mouse; D018796:Immunoconjugates; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D000069594:Abatacept; D002772:Cholera Toxin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin