Preparative ion-exchange chromatography of proteins from dairy whey.
A preparative-scale ion-exchange chromatographic process is described for the separation of the four major proteins and lactose from sweet dairy whey. Experiments using a commercial anion-exchange resin were carried out to determine the optimum conditions for initially separating the proteins alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin, immunoglobulin G and lactose from a sweet dairy whey mixture. The separation was accomplished with simultaneous step elution changes in salt concentration and pH. It was found that the anion-exchange step was most effective in separating beta-lactoglobulin from the feed mixture. Following the anion-exchange separation, its breakthrough curve was processed using a commercial cation-exchange resin to further recover the valuable immunoglobulin G. The whey output from an east Tennessee cheese manufacturer was used as a feedstream for the preparative scale experiments and as a reference in scaling to an economically optimized production level operation.
Gerberding
S J
SJ
Department of Chemical Engineering, University of Tennessee, Knoxville, USA.
Byers
C H
CH
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification; D007526:Isoelectric Point; D007768:Lactalbumin / Q000302:isolation & purification; D007782:Lactoglobulins / Q000302:isolation & purification; D007785:Lactose / Q000302:isolation & purification; D008894:Milk Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification; D000067816:Whey Proteins
D007074:Immunoglobulin G; D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D012710:Serum Albumin, Bovine; D007768:Lactalbumin; D007785:Lactose