title: [Biosynthesis and isolation of a recombinant protein for producing genetically-engineered human proinsulin].
abstract: Isolation of the recombinant protein from a genetically engineered Escherichia coli 1854 producer for further chemical enzymatic transformation into human insulin through proinsulin was studied. Under optimal conditions, the recombinant protein formation was more than 35% of the total cell proteins. Structures of the polypeptides obtained and purified chromatographically were confirmed by amino acid analysis. Human proinsulin was derived from the recombinant protein isolated.
authors.affiliation: All-Russia Institute of Meat Industry, Moscow, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Ivankin
authors.forename: S I
authors.initials: SI
authors.lastname: Mitaleva
authors.forename: A D
authors.initials: AD
authors.lastname: Nekliudov
mesh_terms: D000595:Amino Acid Sequence; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D008969:Molecular Sequence Data; D011384:Proinsulin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D015202:Protein Engineering; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D007328:Insulin; D011993:Recombinant Fusion Proteins; D011384:Proinsulin
vernacular_title: Biosintez i vydelenie rekombinantnogo belka s tsel'iu polucheniia genno-inzhenernogo proinsulina cheloveka.