title: Electron spin resonance spectroscopic detection of oxygen-centred radicals in human serum following exhaustive exercise.
abstract: Free radicals or oxidants are continuously produced in the body as a consequence of normal energy metabolism. The concentration of free radicals, together with lipid peroxidation, increases in some tissues as a physiological response to exercise - they have also been implicated in a variety of pathologies. The biochemical measurement of free radicals has relied in the main on the indirect assay of oxidative stress by-products. This study presents the first use of electron spin resonance (ESR) spectroscopy in conjunction with the spin-trapping technique, to measure directly the production of radical species in the venous blood of healthy human volunteers pre- and post-exhaustive aerobic exercise. Evidence is also presented of increased lipid peroxidation and total antioxidant capacity post-exercise.
authors.affiliation: Department of Health and Exercise Science, School of Applied Sciences, University of Glamorgan, Pontypridd, Wales, UK.
authors.forename: T
authors.initials: T
authors.lastname: Ashton
authors.forename: C C
authors.initials: CC
authors.lastname: Rowlands
authors.forename: E
authors.initials: E
authors.lastname: Jones
authors.forename: I S
authors.initials: IS
authors.lastname: Young
authors.forename: S K
authors.initials: SK
authors.lastname: Jackson
authors.forename: B
authors.initials: B
authors.lastname: Davies
authors.forename: J R
authors.initials: JR
authors.lastname: Peters
mesh_terms: D000293:Adolescent; D000328:Adult; D000975:Antioxidants / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005221:Fatigue / Q000097:blood / Q000503:physiopathology; D005609:Free Radicals / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D010100:Oxygen / Q000502:physiology; D011659:Pulmonary Gas Exchange; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D008054:Lipid Peroxides; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D010100:Oxygen