Does glycosylation of lysosomal proteins show age-related changes in rat liver?
We studied the pattern of lectins binding by liver lysosomal proteins from rats between 18 days of gestation and 72 weeks of age. An analysis of the carbohydrate structure was carried out after an electrophoresis and blotting, followed by a very sensitive detection system with highly specific digoxigenin-labelled lectins. The only age-related differences were observed in the reaction with sialic acid--(MAA; Macckia amurensis, SNA; Sambucus nigra) and fucose--(AAA; Aleuria aurantia) specific lectins. Sialylation increased and fucosylation decreased with age. We also observed a specific reaction with Galanthus nivalis (GNA), Phaseolus vulgaris (PHA-L) and peanut agglutinin (PNA), without any significant changes with age.
Lityńska
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Institute of Zoology, Jagiellonian University, Cracow, Poland. lita@zuk.iz.uj.edu.pl
Przybyło
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D000375:Aging / Q000378:metabolism*; D000818:Animals; D005260:Female; D005333:Fetus / Q000378:metabolism; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism; D027801:Galanthus; D005865:Gestational Age; D006031:Glycosylation; D037102:Lectins; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008247:Lysosomes / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000737:chemistry / Q000378:metabolism; D037121:Plant Lectins; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D054788:Ribosome Inactivating Proteins; D012794:Sialic Acids / Q000737:chemistry / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
D037102:Lectins; D037121:Plant Lectins; D011506:Proteins; C050600:Sambucus nigra lectins; D012794:Sialic Acids; C064549:lectin, Aleuria aurantia; C018589:lysosomal proteins; D005643:Fucose; D005647:Fucosyltransferases; D012799:Sialyltransferases; D054788:Ribosome Inactivating Proteins; D019158:N-Acetylneuraminic Acid