Enzymatic synthesis, isolation, and analysis of novel alpha- and beta-galactosyl-cycloisomalto-octaoses.
Novel branched cycloisomalto-octaoses (CI8s) were enzymatically synthesized by transgalactosylation with alpha-galactosidase from coffee bean and beta-galactosidase preparations from Penicillium multicolor and Bacillus circulans, using melibiose and lactose as donor substrates, and CI8 which is a cyclic homogeneous oligosaccharide composed of eight glucose units bound by alpha-(1-->6)-linkages, as an acceptor. alpha-Galactosyl-CI8s and beta-galactosyl-CI8s obtained were isolated and purified by HPLC. Their structures were elucidated by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDITOFMS) and NMR spectroscopy.
Koizumi
K
K
School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Tanimoto
T
T
Kubota
Y
Y
Kitahata
S
S
D001407:Bacillus / Q000201:enzymology; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003069:Coffee / Q000201:enzymology; D003505:Cyclodextrins / Q000737:chemistry*; D005697:Galactosides / Q000737:chemistry*; D007700:Kinetics; D007785:Lactose / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010407:Penicillium / Q000201:enzymology; D013237:Stereoisomerism; D000519:alpha-Galactosidase / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism; D047408:gamma-Cyclodextrins
D003069:Coffee; D003505:Cyclodextrins; D005697:Galactosides; D047408:gamma-Cyclodextrins; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D007785:Lactose; C023792:gamma-cyclodextrin