Half-embryo cocultivation technique for estimating the susceptibility of pea (Pisum sativum L.) and lentil (Lens culinaris Medik.) cultivars to Agrobacterium tumefaciens.
Longitudinally sliced embryonic axes from pea and lentil mature seeds cocultivated with A. tumefaciens carrying a gus reporter gene in its T-DNA provided a convenient means to evaluate the efficiency of gene transfer to tissues in different cultivars and cocultivation conditions. Use of this technique demonstrated wide variation in susceptibility to Agrobacterium among several pea and lentil commercial genotypes.
Lurquin
P F
PF
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234, USA. lurquin@wsu.edu
Cai
Z
Z
Stiff
C M
CM
Fuerst
E P
EP
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018920:Coculture Techniques; D004269:DNA, Bacterial; D007887:Fabaceae / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D017930:Genes, Reporter; D005822:Genetic Vectors; D018532:Pisum sativum / Q000196:embryology / Q000235:genetics* / Q000382:microbiology; D030821:Plants, Genetically Modified; D010946:Plants, Medicinal; D012639:Seeds / Q000235:genetics / Q000382:microbiology; D013997:Time Factors; D014170:Transformation, Genetic
D004269:DNA, Bacterial; C036483:T-DNA