title: Variations in rat biochemical parameters after buckshot implant.
abstract: Twenty eight albino Wistar rats were implanted with two 100 mg lead spheres: 14 received the implant in the peritoneum (P) and 14 in the thigh (T). Variations in the activity of delta-aminolevulinic dehydratase (ALAD), of urinary delta-aminolevulinic acid (ALAU), of hematoporphyrins (HP) and of lead blood levels (BPb) were then determined at 30, 60 and 90 days with respect to basal values. Parallel determinations were performed by the same schedule in 7 rats implanted with two glass beads and in 8 sham animals receiving surgical incision alone. Techniques employed for ALAD were Berlin and Schaller; for ALAU, Tomokuni and Ogata; for HP, Piomelli; and for BPb, atomic absorption spectrophotometry. As indicators of lead presence, HP and ALAU proved better, both in P and in T rats. The replacement of lead buckshot for small game hunting by other less toxic elements is recommended.
authors.affiliation: Cátedra de Toxicología y Química Legal, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors.forename: C M
authors.initials: CM
authors.lastname: López
authors.forename: A E
authors.initials: AE
authors.lastname: Piñeiro
authors.forename: D E
authors.initials: DE
authors.lastname: González
authors.forename: B
authors.initials: B
authors.lastname: Fernández
authors.forename: L
authors.initials: L
authors.lastname: Bengoechea
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.forename: E C
authors.initials: EC
authors.lastname: Villaamil
authors.forename: M F
authors.initials: MF
authors.lastname: Roberti
authors.forename: J C
authors.initials: JC
authors.lastname: Perazzo
authors.forename: O E
authors.initials: OE
authors.lastname: Roses
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D015745:Granuloma, Foreign-Body / Q000097:blood / Q000209:etiology / Q000378:metabolism; D006415:Hematoporphyrins / Q000378:metabolism; D020007:Implants, Experimental; D007854:Lead / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D014948:Wounds, Gunshot
chemical_list: D006415:Hematoporphyrins; D007854:Lead; D000622:Aminolevulinic Acid