Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis.
The 4.6-kb double-stranded (ds) RNA of Trichomonas vaginalis virus (TVV)-T1 has been shown to encode two overlapping genes, cap and pol. In this study, a serum for specifically detecting viral cap gene product was raised against a recombinant protein, and sera for specifically detecting pol gene product were raised against synthetic oligopeptides. A 75-kDa major protein and a 160-kDa minor protein were detected by anti-CAP serum in a TVV-T1 sample, indicating that the 75-kDa protein is the viral capsid protein. The 160-kDa protein alone was also detected by two distinct anti-POL sera, indicating that the pol gene is expressed as a CAP-POL fusion protein. These results suggest that the TVV-T1 genome is arranged into a cap-pol organization in a manner similar to that of viruses in family Totiviridae.
Liu
H W
HW
Division of Infectious Diseases, Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, Republic of China.
Chu
Y D
YD
Tai
J H
JH
D000818:Animals; D000914:Antibodies, Viral; D002213:Capsid / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D015684:Gene Products, pol / Q000235:genetics / Q000276:immunology; D005814:Genes, Viral; D016679:Genome, Viral; D006801:Humans; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000276:immunology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D019196:Totiviridae / Q000145:classification / Q000235:genetics; D014246:Trichomonas vaginalis / Q000472:pathogenicity / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
D000914:Antibodies, Viral; D015684:Gene Products, pol; D011994:Recombinant Proteins; D014764:Viral Proteins