The CC chemokine 6Ckine binds the CXC chemokine receptor CXCR3.
We cloned the mouse homologue of the chemokine receptor CXCR3, which is located in mouse chromosome X. We screened a large panel of chemokines for their ability to induce a calcium flux in mouse CXCR3-transfected cells and identified a new ligand for this receptor, the recently reported CC chemokine 6Ckine. This represents an example of a CC chemokine, which binds to a CXC chemokine receptor. Like other ligands of this receptor, 6Ckine has angiostatic properties. 6Ckine is known to chemoattract T cells. In line with this, CXCR3 is expressed preferentially in Th1 cells and in lymphoid organs of the IL-10(-/-) mouse that develops chronic colitis. Its ability to attract T cells as well as its angiostatic properties suggest that 6Ckine may be an effective anti-tumor agent.
Soto
H
H
DNAX Research Institute for Cellular and Molecular Biology, Palo Alto, CA 94304, USA.
Wang
W
W
Strieter
R M
RM
Copeland
N G
NG
Gilbert
D J
DJ
Jenkins
N A
NA
Hedrick
J
J
Zlotnik
A
A
D000595:Amino Acid Sequence; D000818:Animals; D054421:Chemokine CCL21; D019742:Chemokines, CC / Q000276:immunology / Q000378:metabolism*; D002633:Chemotaxis / Q000276:immunology; D003001:Cloning, Molecular; D051379:Mice; D008969:Molecular Sequence Data; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016415:Sequence Alignment; D013601:T-Lymphocytes / Q000276:immunology*
C515479:Ccl21c protein, mouse; D054421:Chemokine CCL21; D019742:Chemokines, CC; C515188:Cxcr3 protein, mouse; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine