Identification of the repressor-encoding gene of the Lactobacillus bacteriophage A2.
The repressor gene of the Lactobacillus phage A2 has the following properties: it (i) encodes a 224-residue polypeptide with DNA binding and RecA cleavage motifs, (ii) is expressed in lysogenic cultures, and (iii) confers superinfection immunity on the host. Adjacent, but divergently transcribed, lies another open reading frame whose product resembles the lambda Cro protein. In the 161-bp intergenic segment, putative promoters and operators have been detected.
Ladero
V
V
Area de Microbiología, Universidad de Oviedo, Spain.
García
P
P
Bascarán
V
V
Herrero
M
M
Alvarez
M A
MA
Suárez
J E
JE
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D005814:Genes, Viral; D007778:Lactobacillus / Q000821:virology*; D008242:Lysogeny; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015183:Restriction Mapping; D012270:Ribosomes / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D010948:Viral Plaque Assay; D015678:Viral Structural Proteins / Q000235:genetics*
D012097:Repressor Proteins; D015678:Viral Structural Proteins