Performance limits of low-temperature, continuous locomotion are exceeded when locomotion is intermittent in the ghost crab.
Since a decline in temperature decreases aerobic capacity and slows the kinetics of exercise-to-rest transitions in ectotherms, we manipulated body temperature to better understand the performance limits of intermittent locomotion. Distance capacity (i.e., the total distance traveled before fatigue) of the ghost crab, Ocypode quadrata, was determined during acute exposure to 15 degrees C inside a treadmill-respirometer. Instead of exacerbating the near-paralyzing effects of low body temperature resulting from the frequent transitions, intermittent locomotion allowed animals to exceed the performance limits measured during steady-state locomotion. At low temperature, distance capacity for continuous locomotion at 0.04 m s(-1) (83% maximum aerobic speed) was 60 m. When 30 s of exercise at 0.08 m s(-1) (166% maximum aerobic speed) was alternated with 30 s of rest, distance capacity increased to 271 m, 4.5-fold greater than continuous locomotion at the same average speed (83% maximum aerobic speed). A 30-s pause following a 30-s exercise period was sufficient for maintaining low lactate concentrations in muscle and for partial resynthesis of arginine phosphate. A greater dependency on nonoxidative metabolism due to slowed oxygen uptake kinetics at low temperature resulted in a decreased duration of the critical exercise period, which increased performance relative to that measured at higher temperatures (30 s at 15 degrees C vs. 120 s at 24 degrees C). Despite the ghost crab's limited aerobic capacity at 15 degrees C, distance capacity during intermittent locomotion at low temperature can be comparable to that of a crab moving continuously at a body temperature 10 degrees C warmer. While endurance capacity is generally correlated with maximum aerobic speed, we have demonstrated that both locomotor behavior and body temperature must be considered when characterizing performance limits.
Weinstein
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Department of Integrative Biology, University of California, Berkeley 94720, USA. randiw@bas.cs.arizona.edu
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D000064:Acclimatization; D000255:Adenosine Triphosphate / Q000378:metabolism; D000332:Aerobiosis; D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D003386:Brachyura / Q000502:physiology*; D003080:Cold Temperature; D005080:Exercise Test; D006019:Glycolysis; D007773:Lactates / Q000378:metabolism; D008124:Locomotion / Q000502:physiology*; D018482:Muscle, Skeletal / Q000502:physiology; D009943:Organophosphorus Compounds / Q000378:metabolism; D010101:Oxygen Consumption; D005082:Physical Exertion; D013997:Time Factors
D007773:Lactates; D009943:Organophosphorus Compounds; C015441:phospho-L-arginine; D000255:Adenosine Triphosphate; D001120:Arginine