Ouabain induces apoptosis on PHA-activated lymphocytes.
Apoptotic cell death plays a critical role in immune system homeostasis, and c-myc protooncogene deregulated expression is a component of this programmed genomic response. Pharmacological intervention and modulation of peripheral lymphocytes apoptosis would have important implications. The present results indicate that ouabain, a specific inhibitor of Na+K(+)-ATPase, promotes an increased expression of c-myc mRNA, and induces apoptosis in PHA-stimulated lymphocytes. Furthermore, this ouabain-induced apoptosis cannot be counteracted by the addition of exogenous IL-2.
Olej
B
B
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Brazil.
dos Santos
N F
NF
Leal
L
L
Rumjanek
V M
VM
D017209:Apoptosis; D002455:Cell Division; D003593:Cytoplasm / Q000378:metabolism; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger
D004791:Enzyme Inhibitors; D010835:Phytohemagglutinins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D010042:Ouabain