Purification of a 5-HT uptake inhibitor from the venom of Cerastes vipera.
A protein that inhibits the re-uptake of 5-hydroxytryptamine into rat brain synaptosomes was isolated from the venom of the Sahara sand viper (Cerastes vipera) by gel filtration and reverse phase chromatography. It has a molecular weight of 13,739 Da and an IC50 of about 50 nM for blocking uptake of 3H-5-HT into rat brain synaptosomes. It also augmented the responses to 5-HT in a smooth muscle preparation. It has phospholipase A2 activity, but it has no lytic activity as measured by its inability to release lactate dehydrogenase from rat brain synaptosomes. Determination of the N-terminal sequence revealed a similarity with a phospholipase A2 previously isolated from Cerastes cerastes venom.
Alzahaby
M
M
Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, UK.
Harvey
A L
AL
Young
L C
LC
Faure
G
G
Rowan
E G
EG
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D008297:Male; D008969:Molecular Sequence Data; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000378:metabolism* / Q000494:pharmacology; D017367:Selective Serotonin Reuptake Inhibitors / Q000302:isolation & purification*; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism; D014757:Viper Venoms / Q000032:analysis* / Q000494:pharmacology; D017819:Viperidae
D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D014757:Viper Venoms