Cloning and sequencing of the cDNA coding for pig pre-uteroglobin/Clara cell 10 kDa protein.
Using reverse transcription-polymerase chain reaction (RT-PCR) on pig lung mRNAs, we have cloned and sequenced an almost full-length complementary DNA (cDNA) coding for pig pre-uteroglobin/Clara cell 10 kDa protein (UG/CC10), a major secretory protein of lung Clara cells. The deduced amino acid sequence indicated a preprotein of 91 residues, 21 of which corresponded to the signal peptide. Comparison of the sequence with those of known pre-UG/CC10 from other species indicated that the pig protein resembles the structure shared by human and Lagomorpha pre-UG/CC10 but differ from the proteins from Rodentia that are composed of 96 aminoacids and contain signal peptides of 19 residues. Some amino acids, that form part of a hydrophobic pocket inside the mature protein, are well conserved in all UG/CC10 suggesting an important function of this cavity. Northern analysis indicated that pig UG/CC10 mRNA is abundant in lung but is not detectable in liver, uterus or epididymis. The results are discussed in relation to a possible physiological function of UG/CC10.
Gutiérrez Sagal
R
R
Centro de Biología Molecular Severo Ochoa (C.S.I.C./U.A.M.)., Universidad Autónoma de Madrid, Spain.
Nieto
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A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D000882:Haplorhini; D006801:Humans; D008168:Lung / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment; D013552:Swine; D014598:Uteroglobin / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification
D018076:DNA, Complementary; D011498:Protein Precursors; D011506:Proteins; C556389:SCGB1A1 protein, human; C556390:Scgb1a1 protein, mouse; C022275:pre-uteroglobin; D014598:Uteroglobin