title: Development of human primordial follicles to antral stages in SCID/hpg mice stimulated with follicle stimulating hormone.
abstract: In contrast to the many detailed studies of Graafian follicles, the biology of small follicles in the human ovary is poorly understood and the trigger for follicular growth initiation remains unknown. No practical model exists to study preantral follicle growth in the human because of their slow growth rate and lack of an effective culture system. We therefore tested ovarian xenografts as a new strategy to study the early stages of ovarian follicular growth in vivo. Mice homozygous for severe combined immunodeficiency (SCID) and hypogonadism (hpg) received human ovarian xenografts under their kidney capsules. Follicle growth was assessed by morphology and proliferating cell nuclear antigen (PCNA) immunostaining. The grafts were recovered after 11 (short-term) and 17 weeks (long-term), and serially sectioned. During the last 6 weeks of long-term grafting, mice were randomized to receive either placebo or 1 IU of purified follicle stimulating hormone (FSH) s.c. on alternating days. After 11 weeks of grafting, the most advanced follicles had a maximum of two granulosa cell layers. In the absence of FSH administration, follicles did not progress beyond the two-layer stage even after 17 weeks of grafting, and the oestradiol levels remained undetectable. In the FSH-treated long-term grafts, follicles had grown to antral stages and resulted in oestradiol levels as high as 2070 pmol/l. Growth initiation indices did not differ between control and FSH-treated grafts. This study demonstrates that follicles can survive and grow in human ovarian tissue grafted under the renal capsules of immunodeficient mice for at least 17 weeks, and indicate that xenograft models are potentially useful for studying human follicle development. Using this physiological model, we showed that FSH is required for follicle growth beyond the two-layer stage, although growth initiation is independent of gonadotrophin stimulation.
authors.affiliation: Centre for Reproduction, Growth & Development, School of Medicine, University of Leeds, West Yorkshire, UK.
authors.forename: K
authors.initials: K
authors.lastname: Oktay
authors.forename: H
authors.initials: H
authors.lastname: Newton
authors.forename: J
authors.initials: J
authors.lastname: Mullan
authors.forename: R G
authors.initials: RG
authors.lastname: Gosden
mesh_terms: D000818:Animals; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D006801:Humans; D007006:Hypogonadism / Q000235:genetics; D007150:Immunohistochemistry; D051379:Mice; D008817:Mice, Mutant Strains; D016513:Mice, SCID; D008954:Models, Biological; D006080:Ovarian Follicle / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D005640:Follicle Stimulating Hormone