title: [Riboflavin Reference Standard (Control 951) of National Institute of Health Sciences].
abstract: The raw material for riboflavin was tested for preparation of the "Riboflavin Reference Standard (Control 951)" of National Institute of Health Sciences. Analytical data obtained were as follows: melting point, 284.6 degrees C (decomposition): specific absorbance, E1cm1% = 857 (267 nm), 277 (373 nm), 326 (445 nm); IR spectrum, the same as that of JP Riboflavin Reference Standard (Control 921); optical rotation, [alpha]20D = -135.6 degrees; thin-layer chromatography, three impurities were detected; high-performance liquid chromatography, a small amount of 10 impurities were detected: loss on drying, 0.10%; assay, 100.4% by spectrophotometry. Based on the above results, the raw material was authorized as the Riboflavin Reference Standard (Control 951) of National Institute of Health Sciences (Japanese Pharmacopoeia).
authors.forename: A
authors.initials: A
authors.lastname: Kitajima
authors.forename: Y
authors.initials: Y
authors.lastname: Tagashira
authors.forename: K
authors.initials: K
authors.lastname: Maekawa
authors.forename: K
authors.initials: K
authors.lastname: Yoshii
authors.forename: H
authors.initials: H
authors.lastname: Komatsu
authors.forename: T
authors.initials: T
authors.lastname: Tanimoto
authors.forename: S
authors.initials: S
authors.lastname: Okada
mesh_terms: D007564:Japan; D012015:Reference Standards; D012256:Riboflavin / Q000592:standards*
chemical_list: D012256:Riboflavin