title: The human autoantigen La/SS-B accelerates herpes simplex virus type 1 replication in transfected mouse 3T3 cells.
abstract: Permanently transfected mouse cell lines which expressed different levels of the human autoantigen La/SS-B were infected with different strains of herpes simplex virus type 1, including the strains ANG, HSZP, 17syn+ and HFEM. During infection the localization of the human La protein was followed using an anti-La MoAb, which recognized only the human La protein but did not cross-react with either the endogenous mouse La protein or any viral encoded protein. After infection La protein was transported from the nucleus to the cytoplasm. The time course of translocation was dependent on the amount of human La protein expressed in the respective cell line. Moreover, acceleration of viral replication was dependent on the level of expression of human La protein, suggesting that La protein is a cellular factor that facilitates virus replication.
authors.affiliation: Institut für Physiologische Chemie, Johannes-Gutenberg Universität, Mainz, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Bachmann
authors.forename: H
authors.initials: H
authors.lastname: Deister
authors.forename: A
authors.initials: A
authors.lastname: Pautz
authors.forename: J
authors.initials: J
authors.lastname: Laubinger
authors.forename: M
authors.initials: M
authors.lastname: Schmitz
authors.forename: D
authors.initials: D
authors.lastname: Falke
authors.forename: J
authors.initials: J
authors.lastname: Podlech
authors.forename: D
authors.initials: D
authors.lastname: Grölz
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D005786:Gene Expression Regulation / Q000276:immunology; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D051379:Mice; D012261:Ribonucleoproteins / Q000276:immunology*; D014162:Transfection; D014779:Virus Replication / Q000276:immunology*; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D012261:Ribonucleoproteins; D000097585:SS-B Antigen