title: Identification and cDNA cloning of novel juvenile hormone responsive genes from fat body of the bean bug, Riptortus clavatus by mRNA differential display.
abstract: Juvenile hormone (JH) induces termination of diapause and initiation of reproductive maturation in the adult female bean bug, Riportus clavatus. Applying PCR-based differential display, we have identified four novel JH-responsive cDNAS, that is, three repressible (JR-1, 2 and 3) and one inducible (JI-1). These DNA fragments were partially sequenced and compared with sequences in the data base. JR-3 was shown to have similarity to the transferrins of other insects, which have been reported as JH-suppressed genes. JI-1 has similarity to vitellogenin of Aedes aegypti. On the other hand, JR-1-1 and 2 have no significant similarity to other known sequences. For JR-1, the full cDNA sequence was determined: it contained 913 bp, encoding 194 amino acid residues with a calculated M.W. of 21,531 Da in the mature protein. A total of six JH-responsive genes or cDNAs (four suppressible and two inducible or stimulated), including the already isolated JH-responsive cyanoprotein genes (CP-alpha and beta), have been isolated and are available for further comparative analysis of gene structure and regulatory mechanisms in the same tissue under the same hormonal conditions.
authors.affiliation: Department of Medical Zoology, School of Medicine, Mie University, Tsu, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hirai
authors.forename: M
authors.initials: M
authors.lastname: Yuda
authors.forename: T
authors.initials: T
authors.lastname: Shinoda
authors.forename: Y
authors.initials: Y
authors.lastname: Chinzei
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D005216:Fat Body / Q000378:metabolism; D005260:Female; D017344:Genes, Insect / Q000187:drug effects*; D006430:Hemiptera / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics; D007605:Juvenile Hormones / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D019476:Insect Proteins; D007605:Juvenile Hormones; D012333:RNA, Messenger