Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands.
Several thiophilic adsorbents with mercaptoheterocyclic ligands have been analyzed for their ability to bind human serum proteins in a salt-independent way. In contrast to 2-mercaptopyrimidine, 2-mercaptopyridine derived ligands show a group-selective binding of immunoglobulins and alpha2-macroglobulin, not only in the presence of high concentrations of sodium sulphate but in buffers with low ionic strength. The binding is restricted to thiophilic gels obtained by coupling 2-mercaptopyridine to a vinylsulphone-activated matrix and is not achieved on epichlorohydrin-activated gels. A novel thiophilic ligand based on mercaptonicotinic acid, containing a carboxylic group together with the thiophilic pattern of thioaromatic adsorbents, is demonstrated to be useful as an alternative purification scheme for antibodies.
Scholz
G H
GH
Department of Internal Medicine III, University of Leipzig, Germany.
Wippich
P
P
Leistner
S
S
Huse
K
K
D000327:Adsorption; D000906:Antibodies / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D001798:Blood Proteins / Q000737:chemistry* / Q000302:isolation & purification; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005782:Gels; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification; D008024:Ligands; D011725:Pyridines / Q000737:chemistry*; D011743:Pyrimidines / Q000737:chemistry*; D012492:Salts; D012685:Sepharose / Q000737:chemistry; D000511:alpha-Macroglobulins / Q000737:chemistry / Q000302:isolation & purification
D000906:Antibodies; D001798:Blood Proteins; D005782:Gels; D007074:Immunoglobulin G; D008024:Ligands; D011725:Pyridines; D011743:Pyrimidines; D012492:Salts; D000511:alpha-Macroglobulins; D012685:Sepharose; C029347:2-thiopyridine