[A multistage metrologic approach to the problem of evaluation of the results of the quantitative enzyme immunoassay determination of antitoxic antibodies].
To evaluate a kit for the enzyme immunoassay (ELISA), the metrological approach was used: the total error of the method for the quantitative determination of antibodies, regarded as a multistage process, was determined as the result of the accumulation of errors made in measurements at different stages. The proposed algorithm made it possible to attest the positive control serum, to determine the total error of measurements, to mark a linear section in a limited range of values on a graduation diagram. This led to obtaining well-grounded results, comparable with the results of the reaction of neutralization at good correlation (lc = 0.9).
Petrov
V F
VF
Tarasevich State Research Institute for Standardization and Control of Medical Biological Preparations, Moscow, Russia.
Nikolaeva
A M
AM
Ivanova
V A
VA
Shobukhova
T S
TS
Tumanova
G M
GM
D000465:Algorithms; D000907:Antibodies, Bacterial / Q000032:analysis*; D004166:Diphtheria Antitoxin / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D016014:Linear Models; D015203:Reproducibility of Results; D013743:Tetanus Antitoxin / Q000276:immunology*
D000907:Antibodies, Bacterial; D004166:Diphtheria Antitoxin; D013743:Tetanus Antitoxin
O mnogoétapnom metrologicheskom podkhode k probleme otsenke rezul'tatov kolichestvennogo immunofermentnogo opredeleniia antitoksicheskikh antitel.