Determination of ropivacaine and [2H3]ropivacaine in biological samples by gas chromatography with nitrogen-phosphorus detection or mass spectrometry.
Bioanalytical methods for determining the total concentration of the new local anaesthetic drug ropivacaine in blood plasma, urine and tissues are presented. Ropivacaine is a drug mainly used in connection with surgery and for post-operative pain relief. The biological samples were prepared using liquid-liquid extraction and analysed using capillary gas chromatography with nitrogen-phosphorus detection or mass spectrometry. The methods are highly selective and reliable with a between-day precision, given as the relative standard deviation, generally below 6%. More than 20000 samples have been analysed using the methods described.
Engman
M
M
Concept Division, Bioanalytical Chemistry, Astra Pain Control, Södertälje, Sweden.
Neidenström
P
P
Norsten-Höög
C
C
Wiklund
S J
SJ
Bondesson
U
U
Arvidsson
T
T
D000577:Amides / Q000032:analysis*; D000779:Anesthetics, Local / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D000077212:Ropivacaine
D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine