Pathogenesis of acute passive murine encephalomyelitis I. Importance of host-derived cells as determined by kinetic analysis.
Kinetics of entry into the CNS of donor- and host-derived T-cells during the onset of acute murine EAE induced by the passive transfer of an encephalitogenic PLP(139-151)-specific T-cell clone was investigated. RT-PCR and spectratypic analysis of total RNA recovered from recipient mice demonstrated the presence in the CNS of donor- and host-derived T-cells 24 h post adoptive transfer. Donor-derived T-cells detected in the CNS decreased days 2-6 post transfer while host-derived T-cells persisted during this time. Beginning 3 days before clinical onset, an increase in the CNS of both T-cell populations was observed which persisted through disease onset. Similar analysis performed on recipients of an nonencephalitogenic PLP(139-151)-specific T-clone demonstrated a transient infiltration of donor- and host-derived T-cells beginning 4 days post transfer (dpt) and returning to background levels by day 7 post transfer. Results presented here suggest the importance of host-derived T-cells in the onset of acute passive murine EAE.
Lyons
J A
JA
Department of Microbiology, Medical College of Wisconsin, Milwaukee 53226, USA. lyonsj@neuro.wustl.edu
Zhao
M L
ML
Fritz
R B
RB
D000208:Acute Disease; D000818:Animals; D002999:Clone Cells; D004247:DNA / Q000032:analysis; D017931:DNA Primers; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000209:etiology* / Q000276:immunology*; D005260:Female; D015870:Gene Expression / Q000276:immunology; D007700:Kinetics; D051379:Mice; D008815:Mice, Inbred Strains; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000637:transplantation*
D017931:DNA Primers; D004247:DNA