title: Biodegradation of the herbicide Diuron in soil by indigenous actinomycetes.
abstract: Three actinomycete strains isolated from soil treated with 2,4-D were able to degrade the herbicide Diuron in vitro. Strain CCT 4916 was the most efficient, degrading up to 37% of applied Diuron (100 mg Kg-1 soil) in 7 days, as measured by HPLC and UV/VIS spectroscopy. All strains showed protease and urease activity; intracellular activity of metapyrocatechase and pyrocatechase were not found. Actinomycete strain CCT 4916 produced manganese peroxidase, which could be potentially related to degradation of Diuron.
authors.affiliation: Biological Chemistry Laboratory, Instituto de Química (IQ), UNICAMP, Campinas, Brazil. elisa@bdt.org.br
authors.forename: E
authors.initials: E
authors.lastname: Esposito
authors.forename: S M
authors.initials: SM
authors.lastname: Paulillo
authors.forename: G P
authors.initials: GP
authors.lastname: Manfio
mesh_terms: D000191:Actinomycetaceae / Q000201:enzymology / Q000378:metabolism*; D000681:Amylases / Q000032:analysis; D001673:Biodegradation, Environmental; D001938:Brazil; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D049308:Dioxygenases; D004237:Diuron / Q000032:analysis / Q000378:metabolism*; D010450:Endopeptidases / Q000032:analysis; D006540:Herbicides / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010105:Oxygenases / Q000032:analysis; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis; D014510:Urease / Q000032:analysis
chemical_list: D006540:Herbicides; D012989:Soil Pollutants; D004237:Diuron; D010105:Oxygenases; D049308:Dioxygenases; D050548:Catechol 1,2-Dioxygenase; D050557:Catechol 2,3-Dioxygenase; D000681:Amylases; D010450:Endopeptidases; D014510:Urease