title: Centromere sequences localize to the nuclear halo of human spermatozoa.
abstract: Chromatin is organized into a series of discrete nuclear matrix-associated and non-nuclear matrix-associated domains. The non-matrix-associated domains consist of loops of DNA that are attached to the proteinaceous nuclear matrix by matrix-associated regions (MARs). Although this organization is well characterized in somatic cells, comparatively little is known of this mode of organizing the genome in the human sperm nucleus. To define this relationship, the interaction of human sperm chromatin with the nuclear matrix was assessed by fluorescence in situ hybridization using specific alpha satellite probes directed to the centromeric regions of chromosomes 13 plus 21 and 18. Hybridization of the centromeric sequences was visualized as segmented, bundled structures that extended from the nuclear core into the halo.
authors.affiliation: Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Yaron
authors.forename: J A
authors.initials: JA
authors.lastname: Kramer
authors.forename: K
authors.initials: K
authors.lastname: Gyi
authors.forename: S A
authors.initials: SA
authors.lastname: Ebrahim
authors.forename: M I
authors.initials: MI
authors.lastname: Evans
authors.forename: M P
authors.initials: MP
authors.lastname: Johnson
authors.forename: S A
authors.initials: SA
authors.lastname: Krawetz
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere; D004247:DNA; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D017422:Sequence Analysis, DNA; D013094:Spermatozoa
chemical_list: D004247:DNA