title: Depth profiles of pulmonary surfactant protein B in phosphatidylcholine bilayers, studied by fluorescence and electron spin resonance spectroscopy.
abstract: Pulmonary surfactant-associated protein B (SP-B) has been isolated from porcine lungs and reconstituted in bilayers of dipalmitoylphosphatidylcholine (DPPC) or egg yolk phosphatidylcholine (PC) to characterize the extent of insertion of the protein into phospholipid bilayers. The parameters for the interaction of SP-B with DPPC or PC using different reconstitution protocols have been estimated from the changes induced in the fluorescence emission spectrum of the single protein tryptophan. All the different reconstituted SP-B-phospholipid preparations studied had similar Kd values for the binding of the protein to the lipids, on the order of a few micromolar. The depth of penetration of SP-B into phospholipid bilayers has been estimated by the parallax method, which compares the relative efficiencies of quenching of the protein fluorescence by a shallow or a deeper spin-labeled phospholipid probe. SP-B tryptophan was found to be located 10-13 A from the center of bilayers, which is consistent with a superficial location of SP-B in phosphatidylcholine membranes. Parallax experiments, as well as resonance energy transfer from SP-B tryptophan to an acceptor probe located in the center of the bilayer, indicate that there are significant differences in the extent of insertion of the protein, depending on the method of reconstitution. SP-B reconstituted from lipid/protein mixtures in organic solvents is inserted more deeply in PC or DPPC bilayers than the protein reconstituted by addition to preformed phospholipid vesicles. These differences in the extent of insertion lead to qualitative and quantitative differences in the effect of the protein on the mobility of the phospholipid acyl chains, as studied by spin-label electron spin resonance (ESR) spectroscopy, and could represent different functional stages in the surfactant cycle.
authors.affiliation: Departmento de Bioquímica y Biología Molecular I, Facultad de Biología, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Cruz
authors.forename: C
authors.initials: C
authors.lastname: Casals
authors.forename: I
authors.initials: I
authors.lastname: Plasencia
authors.forename: D
authors.initials: D
authors.lastname: Marsh
authors.forename: J
authors.initials: J
authors.lastname: Pérez-Gil
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D004735:Energy Transfer; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D011510:Proteolipids / Q000737:chemistry* / Q000378:metabolism; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000378:metabolism; D011663:Pulmonary Surfactants / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013552:Swine; D014364:Tryptophan
chemical_list: D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011510:Proteolipids; D011663:Pulmonary Surfactants; D014364:Tryptophan