A new opioid peptide predicted from cloned cDNAs from skin of Pachymedusa dacnicolor and Agalychnis annae.
We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at position 2. In a related precursor from the skin of Agalychnis annae, the sequence of this peptide is in the 3'-untranslated region of the cloned cDNA. From earlier results we predict that in skin peptides the second residue is D-allo-Ile. We have synthesized this and related peptides with different D-amino acids, and determined their delta agonist activity. The peptide with D-nor-Leu binds with high affinity to delta receptors, while that with D-allo-Ile is about 100 times less active.
Wechselberger
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C
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
Severini
C
C
Kreil
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Negri
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L
D000595:Amino Acid Sequence; D000818:Animals; D001001:Anura / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000187:drug effects / Q000235:genetics*; D018847:Opioid Peptides / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D009842:Oligopeptides; D018847:Opioid Peptides; C060209:deltorphin II, Ala(2)-; C030058:dermorphin