Distinct location and prevalence of alpha-, beta-catenins and gamma-catenin/plakoglobin in developing and denervated skeletal muscle.
We studied the distribution of alpha-catenin, beta-catenin and gamma-catenin/plakoglobin in developing, adult and denervated mouse skeletal muscle. During primary myogenesis, all three catenins present a subsarcolemmal distribution within primary myotubes. During secondary myogenesis they accumulate at myotube-myotube contacts. In contrast to the other catenins, gamma-catenin is strongly expressed in the sarcoplasm. In adult muscle, all three catenins are localized on the presynaptic elements of the neuromuscular junction. In denervated muscles, alpha- and beta-catenins are upregulated like N- and M-cadherin, while the levels of gamma-catenin/plakoglobin remain unchanged. The developmental changes in localization and regulation of alpha- and beta-catenins in muscle compared to gamma-catenin/plakoglobin are suggestive of a privileged association of alpha- and beta-catenins with N- and M-cadherins, while gamma-catenin/plakoglobin appears to be expressed quite independently and must assume a different role during myogenesis.
Cifuentes-Diaz
C
C
INSERM, Neuromodulations Interactireset Neurophathologies, Paris, France.
Goudou
D
D
Mège
R M
RM
Velasco
E
E
Nicolet
M
M
Herrenknecht
K
K
Rubin
L
L
Rieger
F
F
D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000378:metabolism; D002448:Cell Adhesion; D002451:Cell Compartmentation; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D051180:Desmoplakins; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D009045:Motor Endplate / Q000737:chemistry / Q000648:ultrastructure; D009121:Muscle Denervation; D024510:Muscle Development; D009124:Muscle Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000294:innervation / Q000378:metabolism* / Q000648:ultrastructure; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D051185:gamma Catenin
C495267:CTNNB1 protein, mouse; D015820:Cadherins; C495272:Ctnna1 protein, mouse; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; C495293:Jup protein, mouse; D009124:Muscle Proteins; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D051185:gamma Catenin; C070896:M-cadherin