Vasoactive intestinal peptide stimulates rat adrenal glucocorticoid secretion, through an ACTH receptor-dependent activation of the adenylate cyclase signaling pathway.
Vasoactive intestinal peptide (VIP) concentration-dependently enhanced corticosterone and cyclic-AMP release by dispersed rat inner adrenocortical cells. A VIP-receptor antagonist and the ACTH-receptor antagonist corticotropin-inhibiting peptide annulled both adrenocortical-cell responses to VIP, while the protein kinase (PKA) inhibitor H-89 blocked only corticosterone response. Collectively, these findings suggest that VIP stimulates glucocorticoid secretion of rat adrenals, through the aspecific activation of ACTH receptors coupled with the adenylate cyclase/PKA-dependent signaling pathway.
Mazzocchi
G
G
Department of Anatomy, University of Padua, Italy.
Rebuffat
P
P
Gottardo
L
L
Nussdorfer
G G
GG
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D003345:Corticosterone / Q000097:blood / Q000378:metabolism*; D000242:Cyclic AMP / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006727:Hormone Antagonists / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D018043:Receptors, Corticotropin / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013449:Sulfonamides; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*; D015384:Zona Glomerulosa / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*
D004791:Enzyme Inhibitors; D006727:Hormone Antagonists; D007546:Isoquinolines; D010446:Peptide Fragments; D018043:Receptors, Corticotropin; D013449:Sulfonamides; D014660:Vasoactive Intestinal Peptide; C026034:ACTH (7-38); D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; D003345:Corticosterone