title: Differential effect of stress on gastric somatostatin, prostaglandin E and gastrin release in the rat.
abstract: BACKGROUND
  The relationship between gastric mucosal damage induced by stress, peptides present in the gastric mucosa and is not clear. Aim of this study was to determine whether cold-restraint stress affected the release of gastric somatostatin, gastrin and in the isolated perfused stomach preparation.
  
  
  METHODS
  Male Sprague-Dawley rats were used, 12 cold-restraint stressed and 12 unstressed controls. 4 additional unstressed rats were treated with aspirin (100 mg/kg p.o.). After 30 minutes, isolated stomachs were perfused for 50 minutes with Krebs-Ringer buffer added with isoproterenol or carbamylcholine plus somatostatin-14 or carbamylcholine alone, somatostatin, gastrin and prostaglandin E2 release in the portal vein effluent were measured by radioimmuno-assay. Histology of the gastric mucosa was obtained from a further 4 stressed and 4 unstressed rats.
  
  
  RESULTS
  In the stomach from stressed animals, the somatostatin response to isoproterenol and the prostaglandin E2 response to carbamylcholine plus somatostatin were significantly lower than in the controls, whereas gastrin response to carbamylcholine was enhanced by stress. Treatment with aspirin abolished the prostaglandin E2 response to stimulation. Gastric mucosa histology from stressed and unstressed animals showed no significant lesions.
  
  
  CONCLUSIONS
  The inhibition of gastric somatostatin and prostaglandins release coupled to an enhanced acid stimulatory influence appear to antidate gastric mucosal injury and should play a role in the stress ulcer genesis.
authors.affiliation: 2nd Surgical Clinic, University of Rome La Sapienza, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Pizzuto
authors.forename: D
authors.initials: D
authors.lastname: Surgo
authors.forename: M
authors.initials: M
authors.lastname: Clementi
authors.forename: R
authors.initials: R
authors.lastname: Marsico
authors.forename: A
authors.initials: A
authors.lastname: Genco
authors.forename: A
authors.initials: A
authors.lastname: Materia
authors.forename: N
authors.initials: N
authors.lastname: Basso
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis*; D005753:Gastric Mucosa / Q000503:physiopathology; D005755:Gastrins / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D010437:Peptic Ulcer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013004:Somatostatin / Q000096:biosynthesis*; D013270:Stomach / Q000503:physiopathology*; D013312:Stress, Physiological / Q000503:physiopathology*
chemical_list: D005755:Gastrins; D013004:Somatostatin; D015232:Dinoprostone