The gene for component-II of botulinum C2 toxin.
The gene encoding component-II of the Clostridium botulinum C2 toxin was cloned from the chromosomal DNA of C. botulinum type C strain (C)-203U28, and the nucleotide sequence was determined. The gene (bc2h) encodes a protein with 721 amino acid residues and is located at 247 bp downstream of the gene for component-I. The N-terminal 16 amino acids were identical to those obtained by analysis of the purified component-II toxin. The ORF for bc2h had only 39% homology at the amino acid level with the C.perfringens iota-Ib protein and an ATP/GTP binding site which is present in the iota-Ib protein is missing from the protein encoded by bc2h. Both genes had a homologous region that predicts a transmembrane segment.
Kimura
K
K
Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
Kubota
T
T
Ohishi
I
I
Isogai
E
E
Isogai
H
H
Fujii
N
N
D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000737:chemistry / Q000235:genetics*; D003016:Clostridium perfringens / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001905:Botulinum Toxins