title: Identification of lysine-238 of Escherichia coli biotin carboxylase as an ATP-binding residue.
abstract: Escherichia coli biotin carboxylase was affinity labeled with adenosine diphosphopyridoxal to identify its ATP binding site. Lysyl endopeptidase digestion of the modified protein, followed by high performance liquid chromatography separation and amino acid sequencing allowed to identify lysine-238 to be the site of modification. Site-directed mutagenesis of this residue into alanine, arginine or glutamine resulted in mutants with much decreased activity. Lysine-238 seems to interact with the gamma-phosphate group of ATP but is not involved in catalysis.
authors.affiliation: Department of Biochemical Engineering and Science, Kyushu Institute of Technology, Iizuka, Japan. kazuta@bse.kyutech.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Kazuta
authors.forename: E
authors.initials: E
authors.lastname: Tokunaga
authors.forename: E
authors.initials: E
authors.lastname: Aramaki
authors.forename: H
authors.initials: H
authors.lastname: Kondo
mesh_terms: D000244:Adenosine Diphosphate / Q000031:analogs & derivatives; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000345:Affinity Labels; D001665:Binding Sites; D019731:Carbon-Nitrogen Ligases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D008239:Lysine / Q000737:chemistry / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011732:Pyridoxal Phosphate / Q000031:analogs & derivatives; D013329:Structure-Activity Relationship
chemical_list: D000345:Affinity Labels; D011732:Pyridoxal Phosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D019731:Carbon-Nitrogen Ligases; C019791:biotin carboxylase; C048236:pyridoxal 5'-diphospho-5'-adenosine; D008239:Lysine