title: Features of Tri101, the trichothecene 3-O-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum.
abstract: A structural gene of Tri101, which encodes trichothecene 3-O-acetyltransferase, was isolated as a 3-kb XhoI-XbaI fragment from the trichothecene producer Fusarium graminearum strain F15. The gene contained no introns, and the coding region was 0.7-kb downstream of a putative UTP-ammonia ligase gene which obviously is not related to the biosynthesis of trichothecenes. Tri101 was expressed when T-2 toxin was added, but this induction was not dependent on the expression level of Tri6, a transcription activator gene in the trichothecene biosynthetic and regulatory gene cluster.
authors.affiliation: Microbial Toxicology Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. mkimura@postman.riken.go.jp
authors.forename: M
authors.initials: M
authors.lastname: Kimura
authors.forename: Y
authors.initials: Y
authors.lastname: Shingu
authors.forename: K
authors.initials: K
authors.lastname: Yoneyama
authors.forename: I
authors.initials: I
authors.lastname: Yamaguchi
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005670:Fusarium / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
chemical_list: D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase