title: Comparative studies of diagnostic bacteriological methods for the recovery of Salmonella from faecal samples from flocks of layers.
abstract: Several conventional culture procedures were compared for the recovery of Salmonella from faecal samples of layer flocks. The cultural media employed consisted of Rappaport-Vassiliadis and tetrathionate broth for selective enrichment and three different plating media (Brilliant Green, Xylose-Lysine-Desoxycholate and modified semisolid Rappaport-Vassiliadis medium). The initial enrichment and plating procedures were repeated in a four-stage secondary enrichment. Selective enrichment in Rappaport-Vassiliadis or tetrathionate broth followed by plating on modified semisolid Rappaport-Vassiliadis medium (selective motility enrichment) resulted in a significantly higher isolation rate for Salmonella from faecal samples of layers compared to Brilliant Green and Xylose-Lysine-Desoxycholate medium. The selective enrichment yielded additional Salmonella isolates up to the second stage. Further enrichment did not increase the isolation rate.
authors.affiliation: Institut für Geflügelkrankheiten, Justus-Liebig-Universität, Giessen.
authors.forename: C
authors.initials: C
authors.lastname: Braun
authors.forename: V
authors.initials: V
authors.lastname: Kostka
authors.forename: E
authors.initials: E
authors.lastname: Balks
authors.forename: T
authors.initials: T
authors.lastname: Redmann
authors.forename: R
authors.initials: R
authors.lastname: Helmuth
mesh_terms: D000818:Animals; D001434:Bacteriophage Typing; D002645:Chickens; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D011201:Poultry Diseases / Q000175:diagnosis*; D012475:Salmonella / Q000145:classification / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012703:Serotyping
chemical_list: D003470:Culture Media