Automated free-solution isotachophoresis: instrumentation and fractionation of human serum proteins.
An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 microm inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.
Böttcher
A
A
Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
Möllers
C
C
Lackner
K J
KJ
Schmitz
G
G
D001798:Blood Proteins / Q000032:analysis*; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D012996:Solutions
D001798:Blood Proteins; D012996:Solutions