Engineering protein kinases with distinct nucleotide specificities and inhibitor sensitivities by mutation of a single amino acid.
A major goal of signal transduction research is to identify the substrates and roles of the many protein kinases. The task might be simplified by the discovery that the mutation of a single amino acid dramatically alters the nucleotide specificity of protein kinases and their inhibition by a particular class of anti-inflammatory drug.
Cohen
P
P
MRC Protein Phosphorylation Unit Department of Biochemistry University of Dundee MSI/WTB Complex Dow Street, Dundee, DD1 5EH, UK pcohen@bad.dundee.ac.uk
Goedert
M
M
D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism; D015688:Oncogene Protein pp60(v-src) / Q000187:drug effects; D017354:Point Mutation / Q000235:genetics; D015202:Protein Engineering / Q000379:methods*; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics*; D011725:Pyridines / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D013379:Substrate Specificity
D000893:Anti-Inflammatory Agents; D004791:Enzyme Inhibitors; D007093:Imidazoles; D009711:Nucleotides; D011725:Pyridines; D011494:Protein Kinases; D015688:Oncogene Protein pp60(v-src); C093642:SB 203580