Fucosyltransferase-catalyzed formation of L-galactosylated Lewis structures.
The Lewis (alpha 1-3/4) fucosyltransferase isolated from human milk could be used for preparative fucosylations of the disaccharide acceptors Gal(beta 1-3)GlcNAc(beta 1-O)R (at position OH-4) and Gal(beta 1-4)GlcNAc(beta 1-O)R (at position OH-3) [R = (CH2)8COOMe]. As donors GDP-L-Gal and deoxygenated derivatives were used to lead to a series of novel modified trisaccharides of the Lewis(a) and the Lewis(x) type, respectively.
Stangier
K
K
Department of Chemistry, University of Alberta, Edmonton, Canada.
Palcic
M M
MM
Bundle
D R
DR
Hindsgaul
O
O
Thiem
J
J
D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000378:metabolism; D005647:Fucosyltransferases / Q000378:metabolism*; D005697:Galactosides / Q000138:chemical synthesis*; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000201:enzymology; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014312:Trisaccharides / Q000138:chemical synthesis*
D004187:Disaccharides; D005697:Galactosides; D007983:Lewis Blood Group Antigens; D014312:Trisaccharides; D006154:Guanosine Diphosphate Fucose; D005647:Fucosyltransferases