Catecholamine effects on lipolysis and blood flow in human abdominal and femoral adipose tissue.
With the use of the microdialysis method, the present study, performed on young, healthy, nonobese subjects of both genders, compares the effects of locally infused catecholamines on glycerol concentration and blood flow in abdominal (Abd) and femoral (Fem) adipose tissue. Physiological activation of the sympathetic nervous system through active tilt was also investigated. In both genders, extracellular glycerol concentration was higher in Fem than in Abd adipose tissue. Local blood flow was lower in Fem than in Abd adipose tissue. Isoproterenol perfusion increased extracellular glycerol levels, but no differences were found by gender or fat-deposit site. Isoproterenol induced a greater increase in local blood flow in Fem adipose tissue in both genders. Epinephrine and norepinephrine perfusion increased extracellular glycerol and reduced blood flow. No major differences were found according to gender and fat-deposit site. Active tilt increased plasma glycerol, free fatty acid, norepinephrine levels, and extracellular glycerol concentration to the same extent whatever the gender and fat deposit. Thus, Fem adipose tissue is characterized by a higher extracellular glycerol concentration and a lower blood flow than is Abd tissue in men and women. In these tissues, in situ lipolysis and local blood flow were similar in response to adrenergic stimulation.
Millet
L
L
Laboratoire de Pharmacologie Médicale et Clinique, Institut National de la Santé et de la Recherche Médicale, Unité 317, Faculté de Médecine, Université Paul Sabatier, 31073 Toulouse cedex, France.
Barbe
P
P
Lafontan
M
M
Berlan
M
M
Galitzky
J
J
D000005:Abdomen / Q000098:blood supply / Q000502:physiology; D000273:Adipose Tissue / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D000328:Adult; D002395:Catecholamines / Q000097:blood / Q000494:pharmacology*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D005990:Glycerol / Q000097:blood; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007866:Leg / Q000098:blood supply / Q000502:physiology; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D017551:Microdialysis; D011187:Posture / Q000502:physiology; D012039:Regional Blood Flow / Q000187:drug effects; D013566:Sympathomimetics / Q000494:pharmacology
D002395:Catecholamines; D005230:Fatty Acids, Nonesterified; D013566:Sympathomimetics; D007545:Isoproterenol; D005990:Glycerol