title: A role for herpesvirus saimiri orf14 in transformation and persistent infection.
abstract: The product of open reading frame 14 (orf14) of herpesvirus saimiri (HVS) exhibits significant homology with mouse mammary tumor virus superantigen. orf14 encodes a 50-kDa secreted glycoprotein, as shown previously (Z. Yao, E. Maraskovsky, M. K. Spriggs, J. I. Cohen, R. J. Armitage, and M. R. Alderson, J. Immunol. 156:3260-3266, 1996). orf14 expressed from recombinant baculovirus powerfully induces proliferation of CD4-positive cells originating from several different species. To study the role of orf14 in transformation, a mutant form of HVS (HVS Deltaorf14) was constructed with a deletion in the orf14 gene. The transforming potential of HVS Deltaorf14 was tested in cell culture and in common marmosets. Parental HVS subgroup C strain 488 immortalized common marmoset T lymphocytes in vitro to interleukin-2-independent growth, while the HVS Deltaorf14 mutant did not produce such a growth transformation. In addition, HVS Deltaorf14 was nononcogenic in common marmosets. In contrast to other nononcogenic HVS mutant viruses which were repeatedly isolated from peripheral blood mononuclear cells of infected marmosets for more than 5 months, HVS Deltaorf14 did not persist at a high level in vivo. These results demonstrate that orf14 of HVS is not required for replication but is required for transformation and for high-level persistence in vivo.
authors.affiliation: Department of Microbiology and Molecular Genetics, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772-9102, USA.
authors.forename: M
authors.initials: M
authors.lastname: Duboise
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: S
authors.initials: S
authors.lastname: Czajak
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: R
authors.initials: R
authors.lastname: Veazey
authors.forename: R C
authors.initials: RC
authors.lastname: Desrosiers
authors.forename: J U
authors.initials: JU
authors.lastname: Jung
mesh_terms: D000818:Animals; D016645:Aotidae; D002144:Callithrix; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D004195:Disease Models, Animal; D006566:Herpesviridae Infections / Q000276:immunology / Q000821:virology*; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008223:Lymphoma / Q000276:immunology / Q000821:virology*; D008253:Macaca mulatta; D016296:Mutagenesis; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000502:physiology*; D016366:Open Reading Frames; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000502:physiology; D012453:Saimiri; D018411:Spodoptera; D013601:T-Lymphocytes / Q000166:cytology; D014412:Tumor Virus Infections / Q000276:immunology / Q000821:virology*; D017735:Virus Latency
chemical_list: D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; C064364:STP protein, Herpesvirus saimiri