title: Myoglobin as a polymerase chain reaction (PCR) inhibitor: a limitation for PCR from skeletal muscle tissue avoided by the use of Thermus thermophilus polymerase.
abstract: Skeletal muscle tissue contains polymerase chain reaction (PCR) inhibitors that are coextracted by conventional nucleic acid extraction procedures. Myoglobin, a heme-containing molecule, was shown to act as a potent Thermus aquaticus DNA polymerase inhibitor and is likely to be involved in muscle tissue-associated PCR inhibition. The use of Thermus thermophilus DNA polymerase avoids muscle tissue-associated PCR inhibition, and should be used in case of small amounts or instability of the targeted nucleic acid.
authors.affiliation: Groupe d'Etude et de Recherche sur le Nerf et le Muscle (GERMEN), Faculté de Médecine de Créteil-Paris XII, Créteil, France.
authors.forename: L
authors.initials: L
authors.lastname: Bélec
authors.forename: J
authors.initials: J
authors.lastname: Authier
authors.forename: M C
authors.initials: MC
authors.lastname: Eliezer-Vanerot
authors.forename: C
authors.initials: C
authors.lastname: Piédouillet
authors.forename: A S
authors.initials: AS
authors.lastname: Mohamed
authors.forename: R K
authors.initials: RK
authors.lastname: Gherardi
mesh_terms: D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism / Q000494:pharmacology*; D005090:Exodeoxyribonucleases / Q000494:pharmacology; D005347:Fibroblasts / Q000737:chemistry / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000235:genetics; D006801:Humans; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000201:enzymology; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology; D009211:Myoglobin / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D019914:Taq Polymerase / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D004279:DNA, Viral; D009211:Myoglobin; D012333:RNA, Messenger; D005954:Glucosephosphate Dehydrogenase; C093010:DNA polymerase, Thermus thermophilus; D019914:Taq Polymerase; D004259:DNA-Directed DNA Polymerase; D005090:Exodeoxyribonucleases