A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern.
Based on its relationship to very-long-chain acyl-CoA synthetase (VLACS), we have cloned and identified a novel, VLACS-related (VLACSR) cDNA from mouse liver. The 2067-bp open reading frame encodes a 689-amino-acid protein with a predicted molecular mass of 76.2 kDa. The carboxy-terminal 500 amino acids of VLACSR show 48% identity and 70% similarity to mouse VLACS and 43% identity and 60% similarity to mouse fatty acid transporter (FATP), respectively. In addition, a partial cDNA of the human VLACSR ortholog was identified. By Northern blot analysis, a 2.6-kb VLACS mRNA was highly abundant only in mouse liver. Low levels of shorter mRNAs were present in brain, lung, testes, and spleen (2.5 kb) and in skeletal muscle (2.2 kb). In heart, but not in kidney, transcripts undetectable by Northern blot analysis could be demonstrated by RT PCR. Southern blot analysis indicated single-copy VLACSR genes in the mouse and human genomes.
Berger
J
J
Institute of Neurology, University of Vienna, Schwarzspanierstrasse 17, Vienna, A-1090, Austria. johannes.berger@univie.ac.at
Truppe
C
C
Neumann
H
H
Forss-Petter
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D050612:Fatty Acid Transport Proteins; D015870:Gene Expression; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D050612:Fatty Acid Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D011506:Proteins; D012333:RNA, Messenger; D012097:Repressor Proteins; C484819:SLC27A5 protein, human; C493573:SLC27A6 protein, human; D029701:Saccharomyces cerevisiae Proteins; C493572:Slc27a4 protein, mouse; C113098:Slc27a5 protein, mouse; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase