Enzyme and biochemical studies of stonefish (Synanceja trachynis) and soldierfish (Gymnapistes marmoratus) venoms.
Venoms from the scorpaeniformes Synanceja trachynis and Gymnapistes marmoratus were quantitatively analyzed for enzymic activity. S. trachynis venom displayed significantly higher hyaluronidase activity than G. marmoratus venom, and G. marmoratus venom displayed significantly higher levels of esterase, acid phosphatase, alkaline phosphatase and phosphodiesterase activity. No detectable quantities of phospholipase A2 activity were found in G. marmoratus venom. SDS-polyacrylamide gel electrophoresis of S. trachynis venom indicated the presence of 6 protein bands (20 kDa-295 kDa). G. marmoratus venom displayed 8 protein bands (11 kDa-109 kDa).
Hopkins
B J
BJ
Department of Pharmacology, Monash University, Clayton, Vic., Australia.
Hodgson
W C
WC
D000135:Acid Phosphatase / Q000032:analysis / Q000737:chemistry; D000469:Alkaline Phosphatase / Q000032:analysis / Q000737:chemistry; D000818:Animals; D001315:Australia; D002265:Carboxylic Ester Hydrolases / Q000032:analysis / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005397:Fish Venoms / Q000737:chemistry / Q000201:enzymology*; D005400:Fishes, Poisonous; D006821:Hyaluronoglucosaminidase / Q000737:chemistry* / Q000378:metabolism; D010741:Phospholipases A / Q000032:analysis / Q000737:chemistry; D054467:Phospholipases A2; D010727:Phosphoric Diester Hydrolases / Q000032:analysis / Q000737:chemistry
D005397:Fish Venoms; C019724:arginine esterase; D002265:Carboxylic Ester Hydrolases; D010741:Phospholipases A; D054467:Phospholipases A2; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010727:Phosphoric Diester Hydrolases; D006821:Hyaluronoglucosaminidase