title: PRT1 of Arabidopsis thaliana encodes a component of the plant N-end rule pathway.
abstract: Mutants in the PRT1 gene of Arabidopsis thaliana are impaired in the degradation of a normally short-lived intracellular protein that contains a destabilizing N-terminal residue. Proteins bearing such residues are the substrates of an ubiquitin-dependent proteolytic system called the N-end rule pathway. The chromosomal position of PRT1 was determined, and the PRT1 gene was isolated by map-based cloning. The 45-kDa PRT1 protein contains two RING finger domains and one ZZ domain. No other proteins in databases match these characteristics of PRT1. There is, however, a weak similarity to Rad18p of Saccharomyces cerevisiae. The RING finger domains have been found in a number of other proteins that are involved in ubiquitin conjugation, consistent with the proposed role of PRT1 in the plant N-end rule pathway.
authors.affiliation: Institute of Botany, University of Vienna, Rennweg 14, A-1030 Vienna, Austria.
authors.forename: T
authors.initials: T
authors.lastname: Potuschak
authors.forename: S
authors.initials: S
authors.lastname: Stary
authors.forename: P
authors.initials: P
authors.lastname: Schlögelhofer
authors.forename: F
authors.initials: F
authors.lastname: Becker
authors.forename: V
authors.initials: V
authors.lastname: Nejinskaia
authors.forename: A
authors.initials: A
authors.lastname: Bachmair
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics; D017343:Genes, Plant; D008969:Molecular Sequence Data; D009154:Mutation; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017421:Sequence Analysis; D044767:Ubiquitin-Protein Ligases
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D010940:Plant Proteins; C063607:RAD18 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; C113475:PRT1 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases