title: Contributions of linker histones and histone H3 to chromatin structure: scanning force microscopy studies on trypsinized fibers.
abstract: Little is known about the mechanisms that organize linear arrays of nucleosomes into the three-dimensional structures of extended and condensed chromatin fibers. We have earlier defined, from scanning force microscopy (SFM) and mathematical modeling, a set of simple structural determinants of extended fiber morphology, the critical parameters being the entry-exit angle between consecutive linkers and linker length. Here we study the contributions of the structural domains of the linker histones (LHs) and of the N-terminus of histone H3 to extended fiber morphology by SFM imaging of progressively trypsinized chromatin fibers. We find that cleavage of LH tails is associated with a lengthening of the internucleosomal center-to-center distance, and that the somewhat later cleavage of the N-terminus of histone H3 is associated with a flattening of the fiber. The persistence of the "zigzag" fiber morphology, even at the latest stages of trypsin digestion, can be attributed to the retention of the globular domain of LH in the fiber.
authors.affiliation: Institute of Molecular Biology, University of Oregon, Eugene 97403-1229, USA.
authors.forename: S H
authors.initials: SH
authors.lastname: Leuba
authors.forename: C
authors.initials: C
authors.lastname: Bustamante
authors.forename: J
authors.initials: J
authors.lastname: Zlatanova
authors.forename: K
authors.initials: K
authors.lastname: van Holde
mesh_terms: D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure; D006657:Histones / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure*; D006868:Hydrolysis; D007700:Kinetics; D008836:Micrococcal Nuclease; D018625:Microscopy, Atomic Force / Q000379:methods; D014357:Trypsin / Q000378:metabolism*
chemical_list: D002843:Chromatin; D006657:Histones; D008836:Micrococcal Nuclease; D014357:Trypsin