Validation procedures of sedimentation field-flow fractionation techniques for biological applications.
Sedimentation field-flow fractionation (SdFFF) offers great potential for the separation of submicrometer and micrometer-sized species. The availability of commercial instrumentation and the versatility of this method originated its success. At this stage of development, SdFFF techniques are mature enough for use in analytical research, development and even routine work. However, prior to their use, these techniques like any other methodologies, have to be validated. As the application of SdFFF techniques to cell separation is being constantly developed, we have investigated separation performance according to validation rules classically defined for separation methods (chromatography) in the case of cellular materials.
Assidjo
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E
Laboratoire de Chimie Analytique et de Bromatologie, Université de Limoges, Faculté de Pharmacie, France.
Chianéa
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T
Dreyfuss
M F
MF
Cardot
P J
PJ
D001800:Blood Specimen Collection; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D004912:Erythrocytes / Q000166:cytology; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013048:Specimen Handling