title: The PAC protein affects the maturation of specific chloroplast mRNAs in Arabidopsis thaliana.
abstract: The pale cress (pac) mutation arrests chloroplast development at an early stage in Arabidopsis thaliana and leads to a white phenotype. Chlorophyll fluorescence measurements demonstrated that the photosynthetic apparatus was impaired. The mutation did not reduce transcription of nuclear genes with photosynthetic function. However, distinct chloroplast-encoded transcripts were affected. The mutation mainly changed the maturation pattern, but the abundance of specific transcripts was also reduced. The defects observed imply a specific role for PAC in chloroplast mRNA maturation. PAC is encoded by a nuclear gene and is transported into the chloroplast. Therefore PAC may be one of the nucleus-encoded factors that function in plastid mRNA maturation and accumulation.
authors.affiliation: Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Heinrich-Heine-Universität, Düsseldorf, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Meurer
authors.forename: C
authors.initials: C
authors.lastname: Grevelding
authors.forename: P
authors.initials: P
authors.lastname: Westhoff
authors.forename: B
authors.initials: B
authors.lastname: Reiss
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D002736:Chloroplasts / Q000235:genetics* / Q000378:metabolism; D049452:Green Fluorescent Proteins; D045342:Light-Harvesting Protein Complexes; D008164:Luminescent Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000096:biosynthesis / Q000502:physiology; D008970:Molecular Weight; D009154:Mutation; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000502:physiology*; D018087:Plastids / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018749:RNA, Plant; D014158:Transcription, Genetic
chemical_list: D029681:Arabidopsis Proteins; D045342:Light-Harvesting Protein Complexes; D008164:Luminescent Proteins; D008565:Membrane Proteins; C100797:Pac-2 protein, Arabidopsis; D045322:Photosynthetic Reaction Center Complex Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C021261:thylakoid polypeptides; D049452:Green Fluorescent Proteins