Regulation of interleukin-6, and macrophage colony-stimulating factor mRNA levels by histamine in stromal cell line (MC3T3-G2/PA6).
OBJECTIVE
The aim of this study was to examine the role of histamine in regulation of IL-6 and M-CSF gene expression in bone marrow stromal cells. Total cellular synthesis was also estimated for IL-6.


METHODS
The effects of the amine were evaluated using the stromal cell line, MC3T3-G2/PA6 (PA6).


RESULTS
Histamine caused a distinct accumulation of IL-6 mRNA in the cells, whereas it decreased M-CSF transcripts. Both pyridylethylamine, a H1 agonist, and dimaprit, a H2 agonist, caused a large increase in the level of IL-6 mRNA in PA6 cells. The histamine-induced expression of IL-6 mRNA was associated with enhanced secretion of IL-6, as determined by ELISA.


CONCLUSIONS
These results, together with the results of our previous studies, suggest that histamine produced by stromal macrophages differentially regulates the production of IL-6 and M-CSF in other kinds of stromal cells and hence promotes differentiation and/or proliferation of hematopoietic progenitor cells.
Takamatsu
S
S
Bioscience Center, Nagoya University, Chikusa, Japan.
Nakano
K
K
D000818:Animals; D015152:Blotting, Northern; D001854:Bone Marrow Cells / Q000378:metabolism*; D002460:Cell Line; D017259:Dimaprit / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006632:Histamine / Q000494:pharmacology*; D017442:Histamine Agonists / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics*; D016173:Macrophage Colony-Stimulating Factor / Q000235:genetics*; D051379:Mice; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D017154:Stromal Cells / Q000378:metabolism
D017442:Histamine Agonists; D015850:Interleukin-6; D011725:Pyridines; D012333:RNA, Messenger; D016173:Macrophage Colony-Stimulating Factor; D006632:Histamine; C006183:2-(2-aminoethyl)pyridine; D017259:Dimaprit