The analysis of DNA adduct formation, removal and persistence in the common mussel Mytilus edulis exposed to 4-nitroquinoline 1-oxide.
32P-postlabelling was used for the detailed analysis of 4-nitroquinoline 1-oxide (4-NQO) induced DNA adduct formation, removal and persistence in the marine shellfish Mytilus spp. The results had a number of important implications concerning the use of such DNA adducts as dosimeters of environmental genotoxin exposures. Our studies indicated that the maintenance of the Mytilus specimens under controlled laboratory conditions can result in the induction of 'stress-related adducts' seemingly related to the nature of the experimental set-up. The studies also indicated that the absorption and activation of genotoxins in this species appear to affect the rate of adduct formation, and that the maximum levels of adducts may not necessarily be induced immediately after the cessation of a genotoxin exposure. In addition, Mytilus specimens were shown to possess a significant capacity to remove these genotoxin-induced DNA adducts. The removal of these adducts appeared to be biphasic in nature, with the rapid removal of a large proportion of adducts occurring within 48 h of the cessation of the exposure, followed by a slow rate of adduct removal over the remaining period of the studies. Despite the relatively efficient removal of the majority of these genotoxin-induced DNA adducts, a proportion remained up to 56 days after the initial exposure. The persistence of these genotoxin-DNA adducts, combined with the information on the rates of adduct removal, indicated that under well-defined conditions, such adducts could serve as suitable biomarkers of environmental contamination.
Harvey
J S
JS
Centre for Molecular Genetics and Toxicology, School of Biological Sciences, University of Wales, Swansea, UK. baharvey@swansea.ac.uk
Parry
J M
JM
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity*; D000818:Animals; D049872:Bivalvia / Q000187:drug effects* / Q000235:genetics; D018736:DNA Adducts / Q000378:metabolism*; D004260:DNA Repair / Q000502:physiology; D004784:Environmental Monitoring / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D013997:Time Factors; D014877:Water Pollution, Chemical
D018736:DNA Adducts; D009153:Mutagens; D015112:4-Nitroquinoline-1-oxide