[Cloning and screening on tumor-related genes inactivated in rat hepatoma cells. II. Reversion of malignant phenotype of hepatoma cells].
A rat normal expressing cDNA library was established and subsequently its whole DNA was transfected into CBRH-7919 rat hepatoma cell cultures. With neomycin selection and morphological distinction, several reversed colonies were isolated and subcloned. All these colonies showed decreased growth rates, reduced capacity for foci formation on soft agar and tumorigenicity in nude mice compared to CBRH-7919. Analysis of rescued plasmid cDNA from these colonies is under way.
Hu
Y J
YJ
Shanghai Institute of Cell Biology, Academia Sinica.
Xu
L
L
Shi
C
C
Liu
J W
JW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015723:Gene Library; D008114:Liver Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009355:Neomycin / Q000494:pharmacology; D009368:Neoplasm Transplantation; D009857:Oncogenes; D010641:Phenotype; D051381:Rats; D017208:Rats, Wistar; D014162:Transfection; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000900:Anti-Bacterial Agents; D009355:Neomycin