Unique pharmacological interactions of taurine and chelerythrine in the retina.
The effects of taurine and chelerythrine (CHT) on ATP-dependent calcium uptake and the phosphorylation of the approximately 20 kDa phosphoprotein in the retina were compared. In the absence of the CHT, taurine stimulated ATP-dependent calcium uptake and attenuated the phosphorylation of the approximately 20 kDa phosphoprotein. On the other hand, CHT produced the opposite results in the absence of taurine. When the two agents were used simultaneously, it was found that CHT non-competitively inhibited the action of taurine to stimulate calcium uptake, while taurine non-competitively inhibited the action of CHT to stimulate the phosphorylation of the approximately 20 kDa phosphoprotein. The data present an unusual pharmacological mechanism for controlling the signal transduction pathway involving the two distinct cellular processes being studied. Given the unique data, a control system is proposed in which the function of the approximately 20 kDa phosphoprotein is linked to the stimulation of ATP-dependent calcium uptake.
Militante
J D
JD
Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock 79430, USA.
Lombardini
J B
JB
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000470:Alkaloids; D000818:Animals; D053119:Benzophenanthridines; D002118:Calcium / Q000378:metabolism; D004336:Drug Antagonism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D010617:Phenanthridines / Q000494:pharmacology*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D051381:Rats; D017208:Rats, Wistar; D012160:Retina / Q000187:drug effects* / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*
D000470:Alkaloids; D053119:Benzophenanthridines; D004791:Enzyme Inhibitors; D010617:Phenanthridines; D010750:Phosphoproteins; D013654:Taurine; C016299:chelerythrine; D011493:Protein Kinase C; D000251:Adenosine Triphosphatases; D002118:Calcium