title: Separation of pd(GC)12 from pd(AT)12 by free solution capillary electrophoresis.
abstract: The two synthetic self complementary oligonucleotides pd(AT)12 and pd(GC)12 were separated by free solution capillary electrophoresis (CZE) using simple borate buffers. The effects of pH (7.5-9) and the concentration of the buffer (0.03-0.35 M) were investigated. Higher pH values and buffer concentrations led to better resolution and longer migration times, the pH having a more pronounced effect on the separation than the concentration of the buffer. It is proposed that the conformation and effective length of the oligonucleotides may have a role in their separation in free solution capillary electrophoresis.
authors.affiliation: Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
authors.forename: I I
authors.initials: II
authors.lastname: Hamdan
authors.forename: G G
authors.initials: GG
authors.lastname: Skellern
authors.forename: R D
authors.initials: RD
authors.lastname: Waigh
mesh_terms: D001888:Boric Acids; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011067:Poly dA-dT / Q000302:isolation & purification*; D011089:Polydeoxyribonucleotides / Q000302:isolation & purification*; D012996:Solutions
chemical_list: D001888:Boric Acids; D002021:Buffers; D011089:Polydeoxyribonucleotides; D012996:Solutions; C016681:poly(dG).poly(dC); D011067:Poly dA-dT; C032688:boric acid