A surface immunodeterminant of human papillomavirus type 16 minor capsid protein L2.
We used human papillomavirus type 16 (HPV-16) particles composed of capsid proteins L1 and L2 (L1/L2 capsids) as an antigen to produce mouse monoclonal antibodies (MAbs). Of 18 MAbs recognizing surface epitopes of L1/L2 capsids, 1 was an anti-L1 MAb and 17 were anti-L2 MAbs. Seven of 11 anti-L2 MAbs recognizing linear epitopes wer found to bind to a synthetic peptide with an HPV-16 L2 sequence of amino acids (aa) 69-81, which is within a highly conserved region among different HPVs. The synthetic peptide reacted with the human sera that had been shown to be positive for an antibody against HPV-16, -18, -58, or -6b capsids composed of L1 alone. The data suggest that the HPV-16 L2 region of aa 69-81 contains a type-common immunodeterminant exposed on the surface of HPV virions.
Kawana
K
K
Division of Molecular Genetics, National Institute of Infectious Diseases, Tokyo, Japan.
Matsumoto
K
K
Yoshikawa
H
H
Taketani
Y
Y
Kawana
T
T
Yoshiike
K
K
Kanda
T
T
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000276:immunology*; D036022:Capsid Proteins; D003429:Cross Reactions; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology*; D051379:Mice; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D027383:Papillomaviridae / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D016056:Immunodominant Epitopes; C063316:L2 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral