Covalent coupling of immunoglobulin G to a poly(vinyl)alcohol-poly(acrylic acid) graft polymer as a method for fabricating the interfacial-recognition layer of a surface plasmon resonance immunosensor.
The synthesis of a terminally thiolated poly(vinyl)alcohol (PVA) grafted with Poly (acrylic acid) (PAA) side chains is described. The PVA-PAA graft polymer (PVAg) was end-tethered to silver surfaces via the terminal thiol functionality and the resultant mobile, hydrophilic polymer matrix exploited for the covalent immobilization of large quantities of polyclonal goat (anti-hIgG) antibody (IgG) with low levels of non-specific adsorption. An SPR immunosensor, fabricated with an IgG-PVA-silver interfacial layer proved capable of performing a sensitive label-free assay of human IgG antigen (hIgG) with minimal non-specific binding interference. A detection limit (DL) for hIgG from serum of 0.8 microgram/ml (5 nM) and an assay sensitivity of 0.66 ng hIgG/mm2/nM are reported.
Disley
D M
DM
Institute of Biotechnology, University of Cambridge, UK.
Blyth
J
J
Cullen
D C
DC
You
H X
HX
Eapen
S
S
Lowe
C R
CR
D000180:Acrylic Resins / Q000737:chemistry*; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D007118:Immunoassay; D007074:Immunoglobulin G / Q000737:chemistry*; D015394:Molecular Structure; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver; D013057:Spectrum Analysis / Q000379:methods*; D013438:Sulfhydryl Compounds
D000180:Acrylic Resins; D007074:Immunoglobulin G; D013438:Sulfhydryl Compounds; D012834:Silver; C006903:carbopol 940; D011142:Polyvinyl Alcohol