Expression of sialylated Lewisx gangliosides in cultured lens epithelial cells from rhesus monkey.
Monkey and human lenses contain essentially the same glycosphingolipids, and Lewisx and sialylated Lewisx epitopes are expressed on the terminal structure of neolactotetraosylceramide. However, monolayer cultures of lens epithelial cells from rhesus monkey expressed gangliosides GM3, GD3 and a small amount of GM1, but not sialylated Lewisx epitopes. Eight-week-old cultures on various extracellular matrices resulted in morphological changes in lens epithelial cells. Monolayer of cells cultured on vitronectin or polylysine assembled into aggregates after 4 weeks of culture. Cells cultured on vitronectin expressed sialyl-Lewisx gangliosides and did not exhibit GD3. On collagens, fibronectin and laminin elongated cells were observed in cells cultured for 8 weeks. Thus, the interaction between cells and extracellular matrices influenced morphology and glycosphingolipid composition in lens epithelial cells.
Ogiso
M
M
Cell and Information, PRESTO, Japan Science and Technology Corporation (JST), Japan.
Takehana
M
M
Kobayashi
S
S
Hoshi
M
M
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism; D005732:Gangliosides / Q000032:analysis / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007797:Laminin; D007908:Lens, Crystalline / Q000166:cytology / Q000378:metabolism*; D016256:Lewis X Antigen / Q000032:analysis; D008253:Macaca mulatta; D008858:Microscopy, Phase-Contrast; D009844:Oligosaccharides / Q000032:analysis; D000080506:Sialyl Lewis X Antigen; D019096:Vitronectin
D005732:Gangliosides; D007797:Laminin; D016256:Lewis X Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen; D019096:Vitronectin; C026226:ganglioside, GD3