Two similar peptides from the venom of the scorpion Pandinus imperator, one highly effective blocker and the other inactive on K+ channels.
Two novel peptides, named Pi4 and Pi7, were purified from the venom of the scorpion Pandinus imperator, and their primary structures were determined. These peptides have 38 amino acids residues, compacted by four disulfide bridges, instead of the normal three found in most K+-channel specific toxins. Both peptides contain 25 identical amino acid residues in equivalent positions (about 66% identity), including all eight half-cystines. Despite the fact that their C-terminal sequence comprising amino acid residues 27 to 37 are highly conserved (10 out of 11 amino acids are identical), Pi4 blocks completely and reversibly Shaker B K+ -channels (a Kv1.1 sub-family type of channel) at 100nM concentration, whereas Pi7 is absolutely inactive at this concentration. Similar effects were observed in binding and displacement experiments to rat brain synaptosomal membranes using 125I-Noxiustoxin, a well known K+-channel specific toxin. In this preparation Pi4 displaces the binding of radiolabeled Noxiustoxin with Ic50 in the order of 10 nM, whereas Pi7 is ineffective at same concentration. Comparative analysis of Pi4 and Pi7 sequences with those obtained by site directed mutagenesis of Charybdotoxin, another very well studied K -channel blocking toxin, shows that the substitution of lysine (in Pi4) for arginine (in Pi7) at position 26, might be one of the important 'point mutations' responsible for such impressive variation in blocking properties of both toxins, here described.
Olamendi-Portugal
T
T
Department of Molecular Recognition and Structural Biology, Institute of Biotechnology, Universidad Nacional Autonoma de Mexico, Cuernavaca.
Gómez-Lagunas
F
F
Gurrola
G B
GB
Possani
L D
LD
D000595:Amino Acid Sequence; D000818:Animals; D001667:Binding, Competitive; D001921:Brain / Q000187:drug effects; D018999:Charybdotoxin / Q000737:chemistry / Q000378:metabolism; D003553:Cystine / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity*; D026902:Potassium Channel Blockers; D051381:Rats; D012604:Scorpion Venoms / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013329:Structure-Activity Relationship; D013574:Synaptosomes / Q000187:drug effects
D004220:Disulfides; D010455:Peptides; C114245:Pi4 peptide; C114246:Pi7 peptide; D026902:Potassium Channel Blockers; D012604:Scorpion Venoms; D018999:Charybdotoxin; D003553:Cystine; C052567:noxiustoxin