Rare, suppurative pulmonary infection caused by Nocardiopsis dassonvillei recognized by glycolipid markers.
An opportunistic actinomycete was isolated as the only etiological agent of a severe, suppurative pulmonary infection. The strain was rapidly recognised as Nocardiopsis by the taxonomically important and immunologically active glycolipid markers (G1 and G2). Identification of the clinical isolate, from a group of actinomycetes mainly known as soil habitants, was definitely proved by chemotaxonomic studies (cell wall/sugar, phospholipid and fatty acid types) as well as by genomic data (GC content, DNA-DNA reassociation). The level of DNA-DNA homology of the clinical actinomycete, in comparison with other reference members of this genus, revealed the highest (88%) relatedness to Nocardiopsis dassonvillei. The results confirmed the value and generic specificity of glycolipid markers from Nocardiopsis, the first time used for rapid recognition of a clinical strain causing a nocardiosis-like disease.
Mordarska
H
H
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław. halimor@immuno.iitd.pan.wroc.pl
Zakrzewska-Czerwiñska
J
J
Paściak
M
M
Szponar
B
B
Rowiñski
S
S
D000193:Actinomycetales Infections / Q000276:immunology / Q000382:microbiology*; D000818:Animals; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002855:Chromatography, Thin Layer; D004269:DNA, Bacterial / Q000737:chemistry; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008169:Lung Abscess / Q000276:immunology / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D009894:Opportunistic Infections / Q000382:microbiology; D011014:Pneumonia / Q000276:immunology / Q000382:microbiology; D011817:Rabbits; D012703:Serotyping; D013306:Streptomycetaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000276:immunology
D004269:DNA, Bacterial; D006017:Glycolipids; D007106:Immune Sera