Genome structure of mycobacteriophage D29: implications for phage evolution.
Mycobacteriophage D29 is a lytic phage that infects both fast and slow-growing mycobacterial species. The complete genome sequence of D29 reveals that it is a close relative of the temperate mycobacteriophage L5, whose sequence has been described previously. The overall organization of the D29 genome is similar to that of L5, although a 3.6 kb deletion removing the repressor gene accounts for the inability of D29 to form lysogens. Comparison of the two genomes shows that they are punctuated by a large number of insertions, deletions, and substitutions of genes, consistent with the genetic mosaicism of lambdoid phages.
Ford
M E
ME
Pittsburgh Bacteriophage Institute, University of Pittsburgh, PA 15260, USA.
Sarkis
G J
GJ
Belanger
A E
AE
Hendrix
R W
RW
Hatfull
G F
GF
D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry*; D019143:Evolution, Molecular; D005814:Genes, Viral / Q000235:genetics* / Q000502:physiology; D016045:Human Genome Project; D008242:Lysogeny; D008969:Molecular Sequence Data; D009160:Mycobacteriophages / Q000235:genetics* / Q000648:ultrastructure; D009161:Mycobacterium / Q000821:virology; D009690:Nucleic Acid Conformation; D010641:Phenotype; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D004279:DNA, Viral; D012367:RNA, Viral; D012343:RNA, Transfer