title: A putative human zinc-finger gene (ZFPL1) on 11q13, highly conserved in the mouse and expressed in exocrine pancreas. The European Consortium on MEN 1.
abstract: In the process of identification of the multiple endocrine neoplasia type 1 gene, which was recently published, we isolated a novel gene in the 11q13 region. This gene (named ZFPL1, for zinc-finger protein-like 1) is expressed strongly in the exocrine pancreas as a 1.4-kb polyadenylated RNA encoding a putative protein of 310 amino acids. A mouse EST contig predicts an equally sized murine protein with 91% amino acid sequence identity to the human protein. No significant homology with known proteins could be found through database screening. However, zinc-finger-like domains and leucine-zipper-like motifs in the predicted ZFPL1 protein were identified, suggesting the presence of DNA-binding and dimerization domains possibly involved in transcription regulation. This notion is supported by the presence of a putative bipartite nuclear localization signal. This paper presents the full-length cDNA sequence for this gene, its genomic structure and chromosomal orientation, and expression studies by Northern blot hybridization and RNA in situ hybridization.
authors.affiliation: Department of Internal Medicine, Utrecht University Hospital, The Netherlands. j.w.m.hoeppener@lab.azu.nl
authors.forename: J W
authors.initials: JW
authors.lastname: Höppener
authors.forename: M J
authors.initials: MJ
authors.lastname: De Wit
authors.forename: A Y
authors.initials: AY
authors.lastname: Simarro-Doorten
authors.forename: J F
authors.initials: JF
authors.lastname: Roijers
authors.forename: H M
authors.initials: HM
authors.lastname: van Herrewaarden
authors.forename: C J
authors.initials: CJ
authors.lastname: Lips
authors.forename: F
authors.initials: F
authors.lastname: Parente
authors.forename: D
authors.initials: D
authors.lastname: Quincey
authors.forename: P
authors.initials: P
authors.lastname: Gaudray
authors.forename: S
authors.initials: S
authors.lastname: Khodaei
authors.forename: G
authors.initials: G
authors.lastname: Weber
authors.forename: B
authors.initials: B
authors.lastname: Teh
authors.forename: F
authors.initials: F
authors.lastname: Farnebo
authors.forename: C
authors.initials: C
authors.lastname: Larsson
authors.forename: C X
authors.initials: CX
authors.lastname: Zhang
authors.forename: A
authors.initials: A
authors.lastname: Calender
authors.forename: A A
authors.initials: AA
authors.lastname: Pannett
authors.forename: S A
authors.initials: SA
authors.lastname: Forbes
authors.forename: J H
authors.initials: JH
authors.lastname: Bassett
authors.forename: R V
authors.initials: RV
authors.lastname: Thakker
authors.forename: I
authors.initials: I
authors.lastname: Lemmens
authors.forename: W J
authors.initials: WJ
authors.lastname: Van de Ven
authors.forename: K
authors.initials: K
authors.lastname: Kas
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics*; D005091:Exons / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007438:Introns / Q000235:genetics; D016350:Leucine Zippers; D051379:Mice; D008969:Molecular Sequence Data; D010179:Pancreas / Q000378:metabolism*; D012313:RNA / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017422:Sequence Analysis, DNA; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C114477:ZFPL1 protein, human; D012313:RNA