title: AtPUMP: an Arabidopsis gene encoding a plant uncoupling mitochondrial protein.
abstract: A cDNA clone (AtPUMP) encoding a plant uncoupling mitochondrial protein was isolated from Arabidopsis thaliana. The cDNA contains an open reading frame of 921 nucleotides encoding 306 amino acids (predicted molecular weight 32,708). The predicted polypeptide is 81% identical and 89% similar to the potato UCP-like protein, and includes an energy transfer protein motif common to mitochondrial transporters. The AtPUMP gene exists as a single copy in the Arabidopsis genome. The corresponding transcript was expressed in all tissues and was strongly induced by cold treatment. We suggest that the putative AtPUMP protein may play a role in heat-requiring physiological events in Arabidopsis.
authors.affiliation: Centro de Biologia Molecular e Engenharia Genética, C.P. 6109, Universidade Estadual de Campinas, SP, Brazil.
authors.forename: I G
authors.initials: IG
authors.lastname: Maia
authors.forename: C E
authors.initials: CE
authors.lastname: Benedetti
authors.forename: A
authors.initials: A
authors.lastname: Leite
authors.forename: S R
authors.initials: SR
authors.lastname: Turcinelli
authors.forename: A E
authors.initials: AE
authors.lastname: Vercesi
authors.forename: P
authors.initials: P
authors.lastname: Arruda
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003080:Cold Temperature; D018076:DNA, Complementary / Q000235:genetics; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007473:Ion Channels; D008565:Membrane Proteins / Q000235:genetics*; D008928:Mitochondria; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1
chemical_list: D002352:Carrier Proteins; D018076:DNA, Complementary; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D010940:Plant Proteins; D014475:Uncoupling Agents; D000071256:Uncoupling Protein 1