Establishment of prespore-specific gene expression in Bacillus subtilis: localization of SpoIIE phosphatase and initiation of compartment-specific proteolysis.
Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation in Bacillus subtilis. Analysis of the distribution of the anti-anti-sigma factor, SpoIIAA, in a variety of mutant backgrounds supports a model in which the SpoIIE phosphatase, which activates SpoIIAA by dephosphorylation, is sequestered onto the prespore face of the asymmetric septum. Thus, prespore-specific gene expression apparently arises as a result of the compartmentalization of SpoIIE protein. The results also suggest the existence of at least two compartment-specific programs of proteolysis, one dependent on the mother cell-specific sigma factor sigma E and the other dependent on the prespore-specific sigma factor sigma F.
Lewis
P J
PJ
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
Wu
L J
LJ
Errington
J
J
D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D001426:Bacterial Proteins / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005838:Genotype; D007700:Kinetics; D016296:Mutagenesis; D010766:Phosphorylation; D012097:Repressor Proteins / Q000378:metabolism; D012808:Sigma Factor / Q000378:metabolism; D013171:Spores, Bacterial / Q000502:physiology; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C064971:FliA protein, Bacteria; D012097:Repressor Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus