Polymorphism in the 5' flanking region of the human somatostatin receptor subtype 5.
The human somatostatin receptor subtype 5 (hSSTR5) gene has previously been cloned and localized to chromosome 16 p13.3. This region is evolutionarily conserved in all vertebrate genomes from the puffer fish (Fugu rubripes) to human, and also contains loci for genes associated with two common multisystemic disorders, adult polycystic kidney disease (PKD1) and tuberous sclerosis (TSC2). Analysis of the 5' flanking region of the hSSTR5 gene has revealed consensus sequences for a number of transcription factors as well as Alu-like repeat elements. In the present study, genomic DNA from 53 unrelated individuals was analysed by PCR and Southern blots probed with radiolabeled fragments generated from different segments of the hSSTR5 gene. We have identified two restriction fragment length polymorphisms (RFLP) with high heterozygosity values at the 5' flanking region of the hSSTR5 gene. These RFLP markers will be useful for determining the allelic loss of genetic material from this region. The observed polymorphism in the promoter region may affect the function of the hSSTR5 gene.
Sasi
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Fraser Laboratories, Department of Medicine, Neurology, Neurosurgery, Pharmacology, Therapeutics, McGill University, Royal Victoria Hospital, the Montreal Neurological Institute, Montreal, Quebec, H3A 1A1, Canada.
Puebla
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Khare
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Patel
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YC
D000328:Adult; D000818:Animals; D001483:Base Sequence; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D019143:Evolution, Molecular; D015870:Gene Expression; D005809:Genes, Regulator; D006801:Humans; D019656:Loss of Heterozygosity; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017481:Receptors, Somatostatin / Q000235:genetics*
D017931:DNA Primers; D017481:Receptors, Somatostatin; C094573:somatostatin receptor 5; D004247:DNA