title: Protein identification by solid phase microextraction-capillary zone electrophoresis-microelectrospray-tandem mass spectrometry.
abstract: We describe an analytical system for the rapid identification of proteins by correlation of tandem mass spectra with protein sequence databases. The system consists of an integrated solid phase microextraction/capillary zone electrophoresis peptide separation device that is connected through a microelectrospray ion source to a tandem mass spectrometer. The limits of detection are 660 amol of sample at a concentration limit of < 33 amol/microliters for peptide mass measurement, and < 10 fmol of sample, at a concentration limit of < 300 amol/microliters for peptide analysis by collision-induced dissociation. Using this system, we have identified low nanogram amounts of yeast proteins separated by high-resolution two-dimensional gel electrophoresis.
authors.affiliation: Department of Molecular Biotechnology, University of Washington, Seattle 98195-7730, USA.
authors.forename: D
authors.initials: D
authors.lastname: Figeys
authors.forename: A
authors.initials: A
authors.lastname: Ducret
authors.forename: J R
authors.initials: JR
authors.lastname: Yates
authors.forename: R
authors.initials: R
authors.lastname: Aebersold
mesh_terms: D016208:Databases, Factual; D019075:Electrophoresis, Capillary / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000737:chemistry
chemical_list: D011506:Proteins