Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease.
The right hand end Nde I fragment 3 (90.8-100 map units) of the fowl adenovirus serotype 10 (FAV-10) was characterised so as to allow the location of an insertion site for recombinant vector construction. Infectious bursal disease virus (IBDV) VP2 gene from the Australian classical strain 002/73, under the control of the FAV-10 major late promoter/leader sequence (MLP/LS) was inserted into a unique Not I site that was generated at 99.5 map units. This recombinant virus was produced without deletion of any portion of the FAV-10 genome. When administered to specific pathogen free (SPF) chickens intravenously, intraperitoneally, subcutaneously or intramuscularly, it was shown that the FAV-10/VP2 recombinant induced a serum VP2 antibody response and protected chickens against challenge with IBDV V877, an intermediate virulent classical strain. Birds were not protected when the recombinant was delivered via the conjunctival sac.
Sheppard
M
M
CSIRO, Division of Animal Health, Animal Health Research Laboratory, Victoria, Australia.
Werner
W
W
Tsatas
E
E
McCoy
R
R
Prowse
S
S
Johnson
M
M
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000259:Aviadenovirus / Q000235:genetics* / Q000276:immunology*; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D007243:Infectious bursal disease virus / Q000235:genetics* / Q000276:immunology* / Q000472:pathogenicity; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000517:prevention & control*; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D015678:Viral Structural Proteins / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage
D000914:Antibodies, Viral; D004279:DNA, Viral; C082513:VP2 protein, infectious bursal disease virus; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines