title: Polymorphic microsatellites in Simulium damnosum s.l. and their use for differentiating two savannah populations: implications for epidemiological studies.
abstract: In West Africa, Onchocerca volvulus, the cause of human onchocerciasis, is transmitted by sibling species of the Simulium damnosum complex. Little is known about blackfly intraspecific variability and its consequences on vectorial capacity. This study reports the use of microsatellite markers for differentiating populations of S. damnosum s.l. Five microsatellite loci were characterized and used to analyze individuals from two savannah populations in Mali, 120 km apart. Four loci were highly polymorphic, having 8-12 alleles per locus and gene diversities ranging from 77.9 to 88.2%. A significant heterozygote deficiency was observed in the two populations. This may arise from inbreeding, population structure (the Walhund effect), or the presence of null alleles. To test this last hypothesis, new primers were designed for two loci and used to analyze homozygous individuals. After correcting for null alleles, heterozygote deficit persisted. Population subdivision in the two foci remains the most likely explanation. Our results indicate that microsatellite markers could differentiate fly populations, making them valuable tools for the study of population genetic structure.
authors.affiliation: Laboratoire d'Epidémiologie des Maladies à Vecteurs, Institut Français pour la Recherche et le Développement en Coopération (ORSTOM), Montpellier, France.
authors.forename: V
authors.initials: V
authors.lastname: Dumas
authors.forename: S
authors.initials: S
authors.lastname: Herder
authors.forename: A
authors.initials: A
authors.lastname: Bebba
authors.forename: C
authors.initials: C
authors.lastname: Cadoux-Barnabé
authors.forename: C
authors.initials: C
authors.lastname: Bellec
authors.forename: G
authors.initials: G
authors.lastname: Cuny
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000737:chemistry; D006801:Humans; D007303:Insect Vectors / Q000235:genetics*; D008302:Mali; D018895:Microsatellite Repeats; D016296:Mutagenesis; D009855:Onchocerciasis / Q000453:epidemiology* / Q000517:prevention & control; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012843:Simuliidae / Q000145:classification / Q000235:genetics*
chemical_list: D004247:DNA