title: A cysteine-11 to serine mutant of G alpha12 impairs activation through the thrombin receptor.
abstract: We have recently reported that G alpha12 is acylated with palmitic acid [Veit et al., FEBS Lett. 339 (1994) 160-164]. Here we identify cysteine 11 as the sole palmitoylation site and assess the function of G alpha12 palmitoylation after expression of wild type and acylation-deficient mutant in insect cells. Our experimental approach yielded the following results. (1) Palmitoylation of G alpha12 has no influence on the subunit interactions. (2) Palmitoylation promotes membrane binding of G alpha12 when this protein is expressed alone. Membrane attachment of the heterotrimer occurs independent of the presence of fatty acids in G alpha12. (3) Assays for agonist-stimulated binding of [35S]GTPgammaS after expression of the human thrombin receptor (PAR1) along with G alpha12 and the betagamma subunits revealed a 70% inhibition with the palmitoyl-deficient mutant.
authors.affiliation: Institut für Immunologie und Molekularbiologie, Freie Universität Berlin, City Campus Veterinary Faculty, Germany.
authors.forename: E
authors.initials: E
authors.lastname: Ponimaskin
authors.forename: C
authors.initials: C
authors.lastname: Harteneck
authors.forename: G
authors.initials: G
authors.lastname: Schultz
authors.forename: M F
authors.initials: MF
authors.lastname: Schmidt
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D003545:Cysteine / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D019308:Palmitic Acid / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D018179:Receptors, Thrombin / Q000378:metabolism*; D012694:Serine / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D018179:Receptors, Thrombin; D019308:Palmitic Acid; D012694:Serine; D003545:Cysteine