title: 31P NMR study of the interactions between oligodeoxynucleotides containing (6-4) photoproduct and Fab fragments of monoclonal antibodies specific for (6-4) photoproduct.
abstract: A 31P nuclear magnetic resonance (NMR) study of the interactions between oligonucleotides containing the (6-4) photoproduct and the Fab fragments of monoclonal antibodies (64M3 and 64M5) recognizing the (6-4) photoproduct is reported. The 31P chemical shift data indicate that backbone conformation of (64) adduct is affected by the presence of flanking oligodeoxynucleotides, and (6-4) adducts with different backbone conformations are accommodated in the antigen binding sites of these antibodies. It was also revealed that epitopes for these antibodies consist of not only the (6-4) adduct but the flanking di- or tri-deoxynucleotides on both the 5' and 3' sides as well.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Torizawa
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: Y
authors.initials: Y
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Asada
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Komatsu
authors.forename: H
authors.initials: H
authors.lastname: Morioka
authors.forename: O
authors.initials: O
authors.lastname: Nikaido
authors.forename: E
authors.initials: E
authors.lastname: Ohtsuka
authors.forename: I
authors.initials: I
authors.lastname: Shimada
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D018736:DNA Adducts; D018604:Epitope Mapping; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D009690:Nucleic Acid Conformation; D010759:Phosphorus Isotopes; D011740:Pyrimidine Dimers / Q000737:chemistry / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D018736:DNA Adducts; D007140:Immunoglobulin Fab Fragments; D010759:Phosphorus Isotopes; D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer