title: IL-10 and IL-4 co-operate to normalize in vitro IgA production in IgA-deficient (IgAD) patients.
abstract: In the present study we evaluated in vitro immunoglobulin production from IgAD individuals and healthy controls. Peripheral blood mononuclear cells (PBMC) from IgAD and controls were cultured with anti-CD40 MoAb presented on a CDw32-transfected fibroblast cell line (CD40 system) in the presence of IL-10, IL-2, IL-4, transforming growth factor-beta (TGF-beta) alone as well as of IL-10 in combination with each of the other three cytokines. Only IL-10 added alone induced significant changes in baseline immunoglobulin production; marked increases in median supernatant levels of all three isotypes were observed in both groups. The most striking finding of this study was the synergizing effect of IL-4 on IgA production in the IgAD group when added with IL-10; median IgA supernatant level increased to a value superimposable on that found in the normal controls which remained about the same as when stimulated with IL-10 alone. The synergic effect of IL-4 and IL-10 was specific to the IgA isotype.
authors.affiliation: Department of Paediatrics, University of Pavia, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Marconi
authors.forename: A
authors.initials: A
authors.lastname: Plebani
authors.forename: M A
authors.initials: MA
authors.lastname: Avanzini
authors.forename: R
authors.initials: R
authors.lastname: Maccario
authors.forename: A
authors.initials: A
authors.lastname: Pistorio
authors.forename: M
authors.initials: M
authors.lastname: Duse
authors.forename: M
authors.initials: M
authors.lastname: Stringa
authors.forename: V
authors.initials: V
authors.lastname: Monafo
mesh_terms: D000293:Adolescent; D000328:Adult; D019013:CD40 Antigens / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004357:Drug Synergism; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D017098:IgA Deficiency / Q000188:drug therapy / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D007376:Interleukin-2 / Q000276:immunology / Q000494:pharmacology; D015847:Interleukin-4 / Q000276:immunology / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D008875:Middle Aged; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000276:immunology / Q000494:pharmacology
chemical_list: D019013:CD40 Antigens; D007376:Interleukin-2; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4