Virus inactivation in superoxide dismutase preparations by ultraviolet light irradiation.
Viral inactivation in superoxide dismutase (SOD) derived from human red cells was carried out by ultraviolet light C (UVC) irradiation. With 400 J/m2 UVC irradiation, the titer of canine parvovirus (CPV, a nonenveloped virus), M13 bacteriophage (M13, a nonenveloped phage) and vesicular stomatitis virus (VSV, an enveloped virus), which were spiked into SOD solution, were reduced by > 4.6 log10 (detection limit), 7.0 log10 and 6.2 log10, respectively. The SOD activity was maintained and the band pattern of SOD on an electrophoresis gel was not changed even by 1000 J/m2 UVC irradiation. These results indicate that UVC irradiation is a promising method for the inactivation of both enveloped and nonenveloped viruses in SOD preparations while maintaining its activity.
Hirayama
J
J
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Abe
H
H
Ikebuchi
K
K
Horiuchi
M
M
Shinagawa
M
M
Kamo
N
N
Sekiguchi
S
S
D000818:Animals; D017104:Bacteriophage M13 / Q000528:radiation effects*; D002522:Chlorocebus aethiops; D004307:Dose-Response Relationship, Radiation; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D017993:Parvovirus, Canine / Q000528:radiation effects*; D013482:Superoxide Dismutase / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000528:radiation effects*
D013482:Superoxide Dismutase