Molecular epidemiology of Mycobacterium bovis isolates from South America.
One hundred seventy-eight isolates of Mycobacterium bovis were subjected to DNA restriction fragment length polymorphism (RFLP) analysis, using the direct repeat element (DR) and the polymorphic GC-rich repeat sequence (PGRS) as probes. By combining the patterns generated by the two repeat DNA elements, 93 different patterns were observed. One hundred-one isolates were grouped in clusters, which include 25 different clusters. One pattern was the most frequently observed, clustering 18.5% of isolates. It was only found in the Center and northeast regions of Argentina and in one isolate from Paraguay. The isolates from Brazil analyzed here presented exclusive patterns (only found in a particular region). The number of exclusive patterns was high in all argentine regions: northeast 78%, center 81%, and Buenos Aires 81%.
Fisanotti
J C
JC
Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria, Morón, Buenos Aires, Argentina.
Alito
A
A
Bigi
F
F
Latini
O
O
Roxo
E
E
Cicuta
E
E
Zumarraga
M J
MJ
Cataldi
A
A
Romano
M I
MI
D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D018900:Dinucleotide Repeats; D005843:Geography; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012091:Repetitive Sequences, Nucleic Acid; D013020:South America / Q000453:epidemiology; D014380:Tuberculosis, Bovine / Q000453:epidemiology*