[Studies on purification and some properties of nisin from Lactococcus lactis subsp. lactis Al2].
Nisin from Lactococcus lactis subsp. lactis AL2 was extracted with n-propanol from NaCl-saturated culture and purified by ion-exchange chromotography on CM-Sephadex C-25. Nisin was purified 1.63 fold with a yield of 41.7%. The molecular weight of nisin was determined by SDS-PAGE to be about 3500. Nisin activity was stable at low pH and sensitive to digestion by a-chymotrypsin. Nisin is capable of inhibiting a broad range of gram-positive bacteria. In contrast, the gram-negative bacteria, yeasts, molds and Nip+ L. lactis subsp. lactis ATCC11454 were not inhibited.
Chen
X
X
Institute of Microbiology, Academia Sinica, Beijing.
He
S
S
Long
L
L
Huan
L
L
Xue
Y
Y
D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D013293:Enterococcus faecalis / Q000187:drug effects; D013294:Lactococcus lactis / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D008837:Micrococcus / Q000187:drug effects; D009561:Nisin / Q000302:isolation & purification* / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D009561:Nisin