[Studies on modulation and control sigma 38 and RMF for the expression of some genes].
The E. coli mutants and wild type strains of rpoS and rmf were cultured in rich medium LB and limited component medium EP respectively. During the stationary phase, the viable cells of mutants were less than wild type strains's. The change of the product of serial proteins was quantified with Western blot. Sigma 38 has not effects on the product of rpoA, rpoB, rpoC, groE and tu gene, depress the transcription of crp and promote the expression of rmf. RMF can promote expression of rpoA, rpoD, groEl, rho, ompA and tufA gene in rich medium, but not in limited medium, and then depress and promote the expression of crp and rpoS respectively.
Ding
Q
Q
Wuhan Institute of Virology, Academia Sinica.
Akira
I
I
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000502:physiology*; D002352:Carrier Proteins; D018833:Chaperonins; D002373:Cyclic AMP Receptor Protein / Q000096:biosynthesis; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000096:biosynthesis / Q000502:physiology*; D012808:Sigma Factor / Q000096:biosynthesis / Q000502:physiology*
D001426:Bacterial Proteins; D002352:Carrier Proteins; D002373:Cyclic AMP Receptor Protein; D029968:Escherichia coli Proteins; C028715:GroE protein, E coli; D006360:Heat-Shock Proteins; D012269:Ribosomal Proteins; D012808:Sigma Factor; C063545:ribosome modulation factor, E coli; C069567:sigma factor KatF protein, Bacteria; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D018833:Chaperonins