title: Pulsed-field gel electrophoresis.
abstract: Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive description of the methodology. Established and novel applications are explored and the reader is provided with an extensive list of references.
authors.affiliation: MRC Human Genetics Unit, Western General Hospital, Edinburgh, Scotland. johnm@hgu.mrc.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Maule
mesh_terms: D004247:DNA / Q000737:chemistry / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000295:instrumentation* / Q000379:methods*
chemical_list: D004247:DNA