Enhancement of the DNA damaging activity of N-nitrosodimethylamine by di-(2-ethylhexyl)phthalate in somatic cells in vivo of Drosophila melanogaster.
The co-genotoxic effect of di-(2-ethylhexyl)phthalate (DEHP) was studied in an in vivo Drosophila genotoxicity test. DEHP is categorized as a non-genotoxic carcinogen. In this study, DEHP also did not show genotoxicity in Drosophila. When larvae were simultaneously treated with DEHP and N-nitrosodimethylamine (NDMA), the DNA damaging activity of NDMA was increased in the Drosophila DNA repair test. Furthermore, DNA double strand breaks were increased by the same treatment of Drosophila. These results suggest that DNA double strand breaks cause the co-genotoxic effect of DEHP in Drosophila.
Kawai
K
K
Faculty of Pharmaceutical Sciences, Meijo University, Nagoya, Japan.
D000818:Animals; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004260:DNA Repair; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004128:Dimethylnitrosamine / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D004357:Drug Synergism; D005260:Female; D017344:Genes, Insect; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D014921:Wings, Animal
D009153:Mutagens; D004247:DNA; D004051:Diethylhexyl Phthalate; D004128:Dimethylnitrosamine