The Effect of the Simultaneous Addition of Molybdenum and Tungsten to the Culture Medium on the Formate Dehydrogenase Activity from Methylobacterium sp. RXM
Shake flask cultivation of the facultative methylotroph Methylobacterium sp. RXM was carried out by using a statistical experimental design to investigate the role of metal association on the formate dehydrogenase (FDH) levels. The maximal values of FDH activity were obtained for tungsten concentration up to 0.6 µM and for molybdenum concentration between 0.6 and 0.9 µM. The negative polynomial parameter (beta2) for tungsten compared with the positive polynomial parameter (beta1) for molybdenum on the FDH activity suggested that the latter metal exerts a stronger influence on the enzyme stimulation than the tungsten metal. A negative interaction between both metals was found, suggesting that tungsten and molybdenum shared an antagonistic effect on the enzyme activity.
Gírio
FM
Instituto Nacional de Engenharia e Tecnologia Industrial, IBTQA, Departamento de Biotecnologia, Unidade de Microbiologia Industrial-Bioprocessos, Azinhaga dos Lameiros, 1699 Lisboa Codex, Portugal
Roseiro
JC
Silva
AI