[The cochlea in short-term culture].
In order to establish organotypic culture of the cochlea, the dissection of the early postnatal mouse cochlea and its morphology in short-term culture were investigated. The viability of hair cells in the culture was assessed using differential interference contrast (DIC) microscopy and double-staining with fluorescein diacetate (FDA) and propidium iodide (PI). The hair cells remained viable and did not show any signs of damage for up to 8-18 hours. When incubated in Hank's solution containing 1 mmol/L dihydrostreptomycin, the hair cells initially deteriorated in 0.5-4.5 hours and progressed to entire degeneration after 10-20 hours. In addition to its utilization in organotypic culture, the cochlea in short-term culture can be used as a valuable model for studying cellular and molecular neurobiology of the inner ears.
Su
Z
Z
PLA General Hospital, Beijing.
Sun
Y
Y
Jiang
S
S
D000818:Animals; D003051:Cochlea / Q000033:anatomy & histology*; D004096:Dihydrostreptomycin Sulfate / Q000633:toxicity; D006198:Hair Cells, Auditory / Q000033:anatomy & histology* / Q000187:drug effects; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009924:Organ Culture Techniques
D004096:Dihydrostreptomycin Sulfate