title: Achiral and chiral high-performance liquid chromatographic methods for clinafloxacin, a fluoroquinolone antibacterial, in human plasma.
abstract: Achiral and chiral HPLC methods were developed for clinafloxacin, a quinolone antimicrobial agent. For achiral assay, analytes were isolated from plasma by precipitating plasma proteins. Separation was achieved on a C18 column using an isocratic eluent of ion pairing solution-acetonitrile (80:20, v/v) at 1.0 ml/min with UV detection at 340 nm. The ion pairing solution was 0.05 M citric acid, 1.15 mM tetrabutylammonium hydroxide and 0.1% ammonium perchlorate. Inter-assay accuracy was within 4.9% with an inter-assay precision of 3.7% over a quantitation range of 0.025 to 10.0 microg/ml. For chiral assay, analytes were isolated from plasma by solid-phase extraction. Separation was achieved on a Crownpak CR(+) column using an isocratic eluent of water-methanol (88:12, v/v) containing 0.1 mM decylamine at 1.0 ml/min with UV detection at 340 nm. Perchloric acid was added to adjust pH to 2. Inter-assay accuracy was within 3.5% with a inter-assay precision of 5.4% over a quantitation range of 0.040 to 2.5 microg/ml.
authors.affiliation: Parke-Davis Pharmaceutical Research Division of Warner-Lambert Company, Department of Pharmacokinetics and Drug Metabolism, Ann Arbor, MI 48105, USA.
authors.forename: J I
authors.initials: JI
authors.lastname: Brodfuehrer
authors.forename: S
authors.initials: S
authors.lastname: Priebe
authors.forename: R
authors.initials: R
authors.lastname: Guttendorf
mesh_terms: D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004355:Drug Stability; D024841:Fluoroquinolones; D006801:Humans; D007262:Infusions, Intravenous; D015363:Quinolones / Q000008:administration & dosage / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; C057477:clinafloxacin