title: Blood dendritic cells from myeloma patients are not infected with Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8).
abstract: In recent studies, the sequence of Kaposi's sarcoma-associated herpes virus (KSHV) or human herpes virus-8 (HHV-8) was detected in dendritic cells (DC) of patients with multiple myeloma (MM). A concern was raised whether there is an causal association between the viral infection and development of these tumors. In the present study, we have examined DC generated from blood adherent cells from 8 Swedish MM patients at different clinical stages and 2 patients with monoclonal gammopathy of undetermined significance. In addition, 6 myeloma cell lines and bone marrow cells from 2 MM patients were also studied. By polymerase chain reaction (PCR), including nested PCR, no virus DNA was demonstrable in the patients' DC or in myeloma cell lines or fresh bone marrow cells. Moreover, no antibody against KSHV was found in the serum of these 10 patients. Thus, our results indicate that blood-derived DC of MM patients in Sweden usually are not infected with KSHV/HHV-8. This study also suggests that KSHV/HHV-8 is not regularly associated with MM and consequently does not play a primary role in the pathogenesis of these tumors.
authors.affiliation: Immunological Research Laboratory, Karolinska Hospital, Stockholm, Sweden. Qing.Yi@cmm.ki.se
authors.forename: Q
authors.initials: Q
authors.lastname: Yi
authors.forename: M
authors.initials: M
authors.lastname: Ekman
authors.forename: D
authors.initials: D
authors.lastname: Anton
authors.forename: S
authors.initials: S
authors.lastname: Bergenbrant
authors.forename: A
authors.initials: A
authors.lastname: Osterborg
authors.forename: P
authors.initials: P
authors.lastname: Georgii-Hemming
authors.forename: G
authors.initials: G
authors.lastname: Holm
authors.forename: K
authors.initials: K
authors.lastname: Nilsson
authors.forename: P
authors.initials: P
authors.lastname: Biberfeld
mesh_terms: D000328:Adult; D000368:Aged; D003713:Dendritic Cells / Q000473:pathology / Q000821:virology*; D005260:Female; D006566:Herpesviridae Infections / Q000097:blood / Q000473:pathology* / Q000821:virology; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009101:Multiple Myeloma / Q000097:blood / Q000473:pathology* / Q000821:virology*; D014407:Tumor Cells, Cultured