title: Epolones: novel sesquiterpene-tropolones from fungus OS-F69284 that induce erythropoietin in human cells.
abstract: In the course of our screening for small molecule modulators of erythropoietin gene expression, two novel sesquiterpene tropolones and pycnidone were isolated from a culture of OS-F69284 (ATCC 74390). Their structures were elucidated by extensive 1H and 13C NMR spectroscopic studies and chemical reactions. These compounds induced erythropoietin gene expression 5-fold at a concentration of 1-1.6 microM.
authors.affiliation: MYCOsearch, a subsidiary of OSI Pharmaceuticals, 4727 University Drive, Suite 400, Durham, North Carolina 27707, USA.
authors.forename: P
authors.initials: P
authors.lastname: Cai
authors.forename: D
authors.initials: D
authors.lastname: Smith
authors.forename: B
authors.initials: B
authors.lastname: Cunningham
authors.forename: S
authors.initials: S
authors.lastname: Brown-Shimer
authors.forename: B
authors.initials: B
authors.lastname: Katz
authors.forename: C
authors.initials: C
authors.lastname: Pearce
authors.forename: D
authors.initials: D
authors.lastname: Venables
authors.forename: D
authors.initials: D
authors.lastname: Houck
mesh_terms: D002240:Carbohydrate Sequence; D002460:Cell Line; D004921:Erythropoietin / Q000096:biosynthesis*; D005658:Fungi / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D012717:Sesquiterpenes; D004921:Erythropoietin