Identification and characterization of RNA-binding activities of avian reovirus non-structural protein sigmaNS.
Cytoplasmic extracts prepared from avian reovirus (ARV) strain S1133-infected chicken embryo fibroblasts were examined for the presence of RNA-binding proteins in order to identify and characterize ARV RNA-binding proteins. Analysis of binding activity to poly(A)-Sepharose indicated that infected cells contained significant amounts of a protein that co-migrated with ARV protein sigmaNS present in total virus-infected cell extracts. Determination of the N-terminal amino acid sequence of several peptide fragments generated by V8 protease digestion of the poly(A)-Sepharose-purified protein confirmed that this viral protein was sigmaNS. Competition assays showed that single-stranded RNA from the unrelated avian pathogen infectious bursal disease virus was able to compete for binding of sigmaNS to poly(A)-Sepharose. These data suggest that ARV sigmaNS binds to single-stranded RNA in a nucleotide sequence non-specific manner and is functionally similar to its counterpart specified by mammalian reovirus.
Yin
H S
HS
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Lee
L H
LH
D000818:Animals; D002642:Chick Embryo; D018165:Orthoreovirus / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D054334:Viral Regulatory and Accessory Proteins
D012330:RNA, Double-Stranded; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; C031657:sigma NS protein, Reovirus; D011061:Poly A; D012313:RNA