Reactivity of human trophoblast monoclonal antibodies with marmoset monkey trophoblast cultures.
The aim of this study was to determine whether cultured trophoblast tissues, derived from the trophectoderm of marmoset monkey blastocysts, contain homologues of human trophoblast antigens. This is an essential prerequisite to determine whether the marmoset may be a suitable model for preclinical testing of a human antitrophoblast antigen for fertility regulation. Previously evaluated monoclonal antibodies from the Flinders University laboratory, which reacted with human trophoblast with a high degree of specificity, were tested for immunohistochemical reactivity using an immunoperoxidase detection method on both frozen and paraformaldehyde-fixed sections of the cultured marmoset monkey trophoblast. All monoclonal antibodies raised against human placenta reacted positively, when compared to controls, suggesting that human and marmoset trophoblast cells share common epitopes. The specificity of the monoclonal antibodies was investigated by determining whether there was cross-reactivity with other marmoset monkey tissues, including adrenal, spleen, kidney, liver, muscle, ovary and testis. The specificities of the monoclonal antibodies on these marmoset tissues were similar to those previously found on the corresponding human tissues. We have concluded that marmoset monkey trophoblast exhibits homologues of human trophoblast antigens. The findings also suggest that marmoset monkeys should be evaluated further as a primate model to test suitable target antigens for antitrophoblast vaccines that may be useful contragestation agents in humans.
Hawes
C S
CS
Department of Obstetrics and Gynaecology, Flinders University of South Australia, Flinders Medical Centre, Bedford Park.
Petropoulos
A
A
Lopata
A
A
Kalionis
B
B
Jones
W R
WR
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002144:Callithrix / Q000276:immunology*; D003269:Contraception, Immunologic; D003429:Cross Reactions; D046508:Culture Techniques; D000939:Epitopes; D005260:Female; D006801:Humans; D008297:Male; D009928:Organ Specificity; D011247:Pregnancy; D013045:Species Specificity; D014327:Trophoblasts / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes