Electron microscopic examination of Aedes albopictus clone C6/36 cells infected with dengue virus 2 at elevated incubation temperature.
Morphological changes such as loss of cytoplasm, membrane destruction and vacuolar swelling in Aedes albopictus clone C6/36 cells infected with dengue virus 2 (DV-2) New Guinea B strain and incubated at 28 degrees C and 37 degrees C, were observed under electron microscope. Both infected and uninfected cells showed significant damage at 37 degrees C in contrast to normal appearance at 28 degrees C. A higher number of virus particles were observed in the cytoplasm at 37 degrees C than at 28 degrees C.
Pandey
B
B
Department of Virology, Nagasaki University, Japan.
Ichinose
A
A
Igarashi
A
A
D000330:Aedes / Q000166:cytology*; D000818:Animals; D002460:Cell Line; D003716:Dengue Virus / Q000502:physiology*; D008854:Microscopy, Electron; D013696:Temperature