Predicted and observed alleles of Plasmodium falciparum merozoite surface protein-1 (MSP-1), a potential malaria vaccine antigen.
The 19-kDa antigenic domain of Plasmodium falciparum merozoite surface protein (MSP)-1 is a potential malaria vaccine candidate. Based on the amino acid substitution, four known alleles, E-TSR (PNG-MAD20 type), E-KNG (Uganda-PA type), Q-KNG (Wellcome type), and Q-TSR (Indo type) of this domain have been identified. Using single or double crossover recombinational events, we predicted the existence of additional alleles of this antigen. The presence of the predicted alleles was determined in parasite isolates from western Kenya, by undertaking a cross-sectional and a longitudinal study. Of the ten predicted alleles, we have revealed the presence of three new alleles: E-KSG-L (Kenya-1 type); E-KSR-L (Kenya-2 type); and E-KNG-F (Kenya-3 type). The results of this study suggest that it may be possible to predict the complexity of the genetic makeup of natural parasite populations.
Qari
S H
SH
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. sxq0@cdc.gov
Shi
Y P
YP
Goldman
I F
IF
Nahlen
B L
BL
Tibayrenc
M
M
Lal
A A
AA
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003430:Cross-Sectional Studies; D016054:DNA, Protozoan; D006801:Humans; D007223:Infant; D007630:Kenya / Q000453:epidemiology; D008137:Longitudinal Studies; D017780:Malaria Vaccines / Q000235:genetics* / Q000276:immunology; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology; D020066:Merozoite Surface Protein 1; D008969:Molecular Sequence Data; D009154:Mutation; D010963:Plasmodium falciparum / Q000235:genetics* / Q000276:immunology; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D016415:Sequence Alignment
D016054:DNA, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011498:Protein Precursors; D015800:Protozoan Proteins