title: The gene 4 of rice yellow stunt rhabdovirus encodes the matrix protein.
abstract: The complete nucleotide sequence of the gene 4 of rice yellow stunt rhabdovirus (RYSV) was determined from cDNAs corresponding to the viral genomic RNA. Gene 4 is 913 nucleotides (nt) long, comprising a 17-nt untranslated 5' region, a 786-nt open reading frame encoding a polypeptide with a molecular mass of 29,125 Da, and a 110-nt untranslated 3' region. Western blot analysis of the RYSV proteins using the antiserum raised against the protein expressed from the cloned gene in Escherichia coli indicates that gene 4 encodes the M protein of RYSV. Comparisons of the deduced amino acid sequence of the M protein of RYSV with those of other rhabdoviruses revealed no significant homologies. However, it shared a similar basic property and a similar distribution of charges with the other rhabdovirus matrix proteins and showed a relatively closer relationship to the sonchus yellow net virus (SYNV) M1 protein.
authors.affiliation: Laboratory of Plant Biotechnology, Chinese Academy of Sciences, Beijing, P.R. China.
authors.forename: Z
authors.initials: Z
authors.lastname: Luo
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.forename: D
authors.initials: D
authors.lastname: Gao
authors.forename: R
authors.initials: R
authors.lastname: Fang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004279:DNA, Viral; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology; D010942:Plant Viruses / Q000235:genetics*; D012209:Rhabdoviridae / Q000235:genetics*; D014763:Viral Matrix Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014763:Viral Matrix Proteins