Electrophoretic characterization of exposed outer membrane proteins in environmental and human Bacteroides fragilis strains.
Bacteriodes fragilis isolated from aquatic environment, from infectious process and from human feces were compared as to their outer membrane protein electrophoretic profiles after staining with Coomassie blue and reacting with antibodies prepared against whole-cell antigens of a reference strain from a clinical source. A marked homogeneity was found among the strains with these methodologies. The profiles of all strains obtained after radio-iodination of the intact cell showed qualitative similarity when compared with the profiles obtained by the other methods. Thus, these data allow us to suggest the designation of the peptides observed in the autoradiograms as surface-exposed proteins. Differences observed in the autoradiograms in the expression of bands mainly detected at a molecular weight of 28 in the commensal strain 118,310 defined previously as avirulent, in addition to a distinction in the titres of agglutination with the sera tested and lower reactivity in the immunoblotting assays, suggest a relationship of the B. fragilis surface architecture with the virulence potential as well as with the origin of the strain.
Domingues
R M
RM
Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brasil.
Avelar
K E
KE
Silva e Souza W das
G
G
Moraes
S R
SR
Antunes
E N
EN
Ferreira
M C
MC
D000372:Agglutination Tests; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001442:Bacteroides Infections / Q000382:microbiology*; D001441:Bacteroides fragilis / Q000737:chemistry* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D011817:Rabbits
D001425:Bacterial Outer Membrane Proteins