title: [Technical note: proposal of a routine method of accurate measurement of urinary trans,trans-muconic acid].
abstract: A method for routine measurement of urinary trans,trans-muconic acid. A method is proposed which allows the accurate determination of urinary trans,trans-muconic acid (Ma) by HPLC with UV detection. Sample pretreatment consists of a first purification on strong anionic exchange (SAX) cartridges followed by extraction on "end capped" reversed-phase (C18-EC) ones. Purified samples are directly injected onto a chromatographic column (C18, 150 x 4.6 mm I.D., 3 microns) which is eluted with water: methanol: acetic acid (97:2:1, v/v) mixture followed by a "spike" of acetonitrile. The retention time of MA is 15 min, the coefficient of variation is lower than 3%, the limit of detection is 7 micrograms/l. The method seems suitable for accurate biological monitoring of subjects exposed to benzene.
authors.affiliation: Fondazione Salvatore Maugeri I.R.C.C.S. Pavia.
authors.forename: L
authors.initials: L
authors.lastname: Maestri
authors.forename: S
authors.initials: S
authors.lastname: Ghittori
authors.forename: P
authors.initials: P
authors.lastname: Marraccini
authors.forename: P
authors.initials: P
authors.lastname: Zadra
authors.forename: M
authors.initials: M
authors.lastname: Imbriani
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D006801:Humans; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D014556:Urine / Q000737:chemistry*
chemical_list: C005939:muconic acid; D013011:Sorbic Acid
vernacular_title: Nota tecnica: proposta di un metodo routinario per il dosaggio accurato dell'acido t,t-muconico nelle urine.