Stimulation of T-cell proliferation by pancreastatin and its C-terminal fragment (33-49).
We have studied the effect of pancreastatin and its C-terminal fragment (33-49) on mitogen-stimulated T lymphocyte proliferation. In a concentration range from 10(-12) to 10(-8) M they exhibit a dose-dependent stimulatory effect on concanavalin A-induced response with the maximal effect at 10(-8) M concentration. They were inactive in response to a B-cell mitogen, lipopolysaccharide, which points to an involvement of T but not B lymphocytes in their response. Pancreastatin can still produce a stimulatory effect when added 18 h after incubation of cultures with concanavalin A and apparently uses a diacylglycerol independent mechanism. When cells were preincubated for 4, 16 or 24 h with pancreastatin or its fragment and then stimulated with concanavalin A, a ten times lower concentration of peptides was needed (10(-9) M) to obtain the maximal response. This suggests that resting cells are more sensitive to pancreastatin and its fragment. Both peptides exhibit a very similar pharmacological profile, indicating that the C-terminal part of the molecule is responsible for the effect on T-cell proliferation.
Haberstock-Debic
H
H
Department of Medicine, University of Antwerpen (UIA), Belgium. haber@olimp.irb.hr
Banfic
H
H
Stevens
W J
WJ
De Clerck
L S
LS
Wechsung
E
E
De Potter
W
W
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D053379:Chromogranin A; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D010187:Pancreatic Hormones / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects; D013552:Swine; D013601:T-Lymphocytes / Q000187:drug effects*
D053379:Chromogranin A; D008070:Lipopolysaccharides; D010187:Pancreatic Hormones; D010446:Peptide Fragments; C073861:pancreastatin (33-49); C050836:pancreastatin; D003208:Concanavalin A