Microenvironment of Morris hepatoma 5123 metastases to the lungs--ultrastructural analysis of intratumour injections of rhTNF-alpha.
We analysed the effect of rhTNF-alpha on the development of Morris hepatoma 5123 spontaneous metastases to the lungs of Buffalo rats. After intratumour administration of the cytokine the lungs were found to contain scarce metastatic foci. In precapillaries and capillaries neoplastic cells were single or in small clusters, without contact with endothelial cells. Endothelial cells showed high pinocytic activity and frequently tightly closed vascular lumen. The stroma of interalveolar septa and the capillary bed contained multiple eosinophils. Moreover, eosinophils, histiocytes and monocytes mixed with abundant collagen fibres were found on the margin of single small metastatic foci and among necrotic neoplastic cells.
Nowak
H F
HF
Department of Pathological Anatomy, Medical Academy of Białystok.
Terlikowski
S
S
Musiatowicz
B
B
Sulkowska
M
M
Sulkowski
S
S
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy / Q000556:secondary* / Q000648:ultrastructure; D008168:Lung / Q000098:blood supply / Q000648:ultrastructure*; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary* / Q000648:ultrastructure; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D051381:Rats; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000008:administration & dosage / Q000627:therapeutic use*
D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha