Characterization of a gene encoding an abscisic acid-inducible type-2 lipid transfer protein from rice.
The cloning and sequence analysis of a novel gene that encodes a type 2 non-specific lipid transfer-like protein (LTP) from rice is reported. Sequence analysis revealed an ORF encoding a protein showing characteristics of the LTP proteins. However, rice LTP2 is more similar to heterologous LTPs than to rice LTP1, supporting the existence of two distinct families of plant LTPs. Ltp2 mRNA is accumulated only in mature seeds. In vegetative tissues, mRNA was only detected after treatment with abscisic acid (ABA), mannitol or NaCl. Transient expression experiments that the 61 nucleotides upstream of the TATA box, containing two ACGT boxes and the motif I, are sufficient for ABA responsiveness of the Ltp gene.
García-Garrido
J M
JM
Laboratoire de Physiologie et Biologie Moléculaire Végétales, UMR 5545 CNRS Université de Perpignan, France.
Menossi
M
M
Puigdoménech
P
P
Martínez-Izquierdo
J A
JA
Delseny
M
M
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012639:Seeds; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D002352:Carrier Proteins; C109356:LTP2 protein, plant; D010940:Plant Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D000040:Abscisic Acid