[Bromodeoxyuridine immunohistochemical observation of sensory epithelium repair after ototoxic destruction in the crista ampullaris of guinea pigs].
In this in vivo study we investigated the proliferation of crista ampullaris in guinea pigs after Gentamicin intoxication by BrdU immunohistochemical observation and semi-thin sections. The experimental animals were given a single intraperitonial injection of BrdU at a dose of 150 mg/kg body weight before subsequently killed at 1, 3, 7, 14 and 22 day post-treatment (PT). Supporting cell proliferation was evident by BrdU immunoreactivity in the chromatin material of dividing cells at 7 and 14 day PT. The results demonstrated that the crista ampullaris of guinea pigs had repaired by supporting cell mitosis.
Lang
H
H
Beijing Institute Otolaryngology.
Liu
C
C
Fan
E
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001973:Bromodeoxyuridine; D002455:Cell Division; D005839:Gentamicins / Q000633:toxicity*; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects*; D007150:Immunohistochemistry; D007760:Labyrinth Supporting Cells / Q000502:physiology; D012444:Saccule and Utricle / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D005839:Gentamicins; D001973:Bromodeoxyuridine