Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases.
Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis.
Sakakibara
Y
Y
Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
Yanagisawa
K
K
Takami
Y
Y
Nakayama
T
T
Suiko
M
M
Liu
M C
MC
D000595:Amino Acid Sequence; D000818:Animals; D015239:Arylsulfotransferase / Q000737:chemistry*; D019556:COS Cells; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry*; D014162:Transfection / Q000235:genetics
D012333:RNA, Messenger; D011994:Recombinant Proteins; D015238:Sulfotransferases; D015239:Arylsulfotransferase; C028152:alcohol sulfotransferase