Functional analysis of upstream activating elements in the promoter of the FBP1 gene from Saccharomyces cerevisiae.
We have investigated the effect of different carbon sources and of different mutations on the capacity of two elements, UAS1 and UAS2, from the promoter of the FBP1 gene to form specific DNA-protein complexes and to activate expression of a reporter gene. The complexes are observed with nuclear extracts from yeast derepressed on glycerol or ethanol. When hxk2 mutants are grown on glucose the nuclear extracts are able to complex UAS1 but not UAS2, while for wild-type cells grown on galactose only the complex with UAS2 is formed. In contrast, in vivo the operation of both UASs is high in ethanol, moderate to low in glycerol, and negligible in galactose; no expression is observed in glucose even in a hxk2 background. There is no effect of a MIG1 deletion, either in the formation of DNA-protein complexes or on the expression of reporter genes.
de Mesquita
J F
JF
Instituto de Investigaciones Biomédicas del CSIC., Arturo Duperier 4, E-28029 Madrid, Spain.
Zaragoza
O
O
Gancedo
J M
JM
D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D000431:Ethanol / Q000494:pharmacology; D006597:Fructose-Bisphosphatase; D005656:Fungal Proteins / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005690:Galactose / Q000494:pharmacology; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects / Q000235:genetics; D005800:Genes, Fungal / Q000187:drug effects / Q000235:genetics*; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000187:drug effects / Q000235:genetics; D012045:Regulatory Sequences, Nucleic Acid / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents / Q000494:pharmacology
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; D000431:Ethanol; C111172:FBP1 protein, S cerevisiae; D006597:Fructose-Bisphosphatase; D005947:Glucose; D005990:Glycerol; D005690:Galactose