title: Solution structure and dynamics of the bioactive retroviral M domain from Rous sarcoma virus.
abstract: A biologically active construct of the retroviral M domain from the avian Rous sarcoma virus is defined and its solution structure described. This M domain is fully active in budding and infectivity without myristylation. In spite of a sequence homology level that suggests no relationship among M domains and the family of matrix proteins in mammalian retroviruses, the conserved structural elements of a central core allow an M domain sequence motif to be described for all retroviruses. The surface of the M domain has a highly clustered positive patch comprised of sequentially distant residues. An analysis of the backbone dynamics, incorporating rotational anisotropy, is used to estimate the thermodynamics of proposed domain oligomerization.
authors.affiliation: The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: McDonnell
authors.forename: D
authors.initials: D
authors.lastname: Fushman
authors.forename: S M
authors.initials: SM
authors.lastname: Cahill
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.forename: A
authors.initials: A
authors.lastname: Wolven
authors.forename: C B
authors.initials: CB
authors.lastname: Wilson
authors.forename: T D
authors.initials: TD
authors.lastname: Nelle
authors.forename: M D
authors.initials: MD
authors.lastname: Resh
authors.forename: J
authors.initials: J
authors.lastname: Wills
authors.forename: D
authors.initials: D
authors.lastname: Cowburn
mesh_terms: D000595:Amino Acid Sequence; D001358:Avian Sarcoma Viruses / Q000737:chemistry*; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012191:Retroviridae Proteins / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D017421:Sequence Analysis; D013329:Structure-Activity Relationship; D014763:Viral Matrix Proteins / Q000737:chemistry* / Q000235:genetics
chemical_list: D012191:Retroviridae Proteins; D014763:Viral Matrix Proteins