Ganglioside GM2 is substrate for a sialidase in MDCK cells.
GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.
Marchesini
S
S
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Benaglia
G
G
Piccinotti
A
A
Bresciani
R
R
Preti
A
A
D000818:Animals; D002460:Cell Line; D004285:Dogs; D005677:G(M1) Ganglioside / Q000378:metabolism; D005678:G(M2) Ganglioside / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism*; D013379:Substrate Specificity
D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; D009439:Neuraminidase