title: pICln predominantly localizes at luminal surface membranes of distal tubules and Henle's ascending limbs.
abstract: We produced a highly specific antibody to the C-terminal peptide sequence of pICln. It recognized pICln with a 38-kDa molecular mass on SDS-polyacrylamide gel electrophoresis, coinciding with that previously reported. During native polyacrylamide gel electrophoresis, three immunoreactive bands (38, 70, and 130 kDa) were detected. The isoelectric point of pICln was calculated to be 4.0. Subcellular localization study showed the presence of pICln in the soluble and microsomal fraction. pICln can be easily solubilized from the membrane fraction with Triton X-100. From immunohistochemical observations, we found pICln to be obviously located on the luminal surface membranes of the distal tubules and Henle's loop ascending limbs, and it can also be found inside proximal tubular cells. The present results suggested that pICln functions as a "cytosolic anchor = membrane insertion" model, and it plays important roles in the "urine dilution segment" cells of nephrons.
authors.affiliation: Department-2 of Biochemistry, School of Medicine, Akita University, Japan.
authors.forename: G Z
authors.initials: GZ
authors.lastname: Tao
authors.forename: A
authors.initials: A
authors.lastname: Komatsuda
authors.forename: A B
authors.initials: AB
authors.lastname: Miura
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Tashima
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D048429:Cell Size / Q000502:physiology; D018118:Chloride Channels / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D007473:Ion Channels; D007526:Isoelectric Point; D007668:Kidney / Q000166:cytology / Q000502:physiology; D007684:Kidney Tubules / Q000737:chemistry*; D008138:Loop of Henle / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D017830:Octoxynol / Q000494:pharmacology; D009997:Osmotic Pressure; D012995:Solubility; D013552:Swine
chemical_list: C410035:CLNS1A protein, human; D018118:Chloride Channels; D007473:Ion Channels; D008565:Membrane Proteins; D017830:Octoxynol