title: Ultrastructural evaluation of lysosomes and biochemical changes in cathepsin D distribution in hepatocytes in methanol intoxication.
abstract: Methanol oxidation is accompanied by free radicals and formaldehyde formation. It is likely to cause damage of lysosomal membranes. Lysosomal ultrastructure under transmission electron microscope and biochemical localization of cathepsin D were estimated after rats intoxication with methanol. The examination was carried out 6, 12 and 24 h and 2.5 and 7 days after intoxication. Ultrastructural examination showed that methanol causes extension of Golgi apparatus cisterns and an increase in a number of lysosomes. From 12 h to 2 days lysosomes were characterized by damage of structure of membrane enclosing lysosomes. During the first days of intoxication activity of cathepsin D decreased in lysosomes and increased in cytosol. These changes may lead to uncontrolled extralysosomal proteolysis in the liver cells and to the onset of liver tissue destruction.
authors.affiliation: Department of Instrumental Analysis, Medical Academy of Białystok.
authors.forename: E
authors.initials: E
authors.lastname: Skrzydlewska
authors.forename: B
authors.initials: B
authors.lastname: Szynaka
mesh_terms: D000818:Animals; D002402:Cathepsin D / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D000432:Methanol / Q000506:poisoning*; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002402:Cathepsin D; D000432:Methanol