title: A novel method for selection of chymotrypsin inhibitors from a phage peptide library.
abstract: A novel screening strategy has been developed for the identification of alpha-chymotrypsin inhibitors from a phage peptide library. In this strategy, the standard affinity selection protocol was modified by adding a proteolytic cleavage period to avoid recovery of alpha-chymotrypsin substrates. After four cycles of selection and further activity assay, a group of related peptides were identified by DNA sequencing. These peptides share a consensus sequence motif as (S/T)RVPR(R/H). Then, a corresponding short peptide (Ac-ASRVPRRG-NH2) was synthesized chemically and proved to be an inhibitor of alpha-chymotrypsin. The present work provides a useful way for searching proteinase inhibitors without detailed knowledge of the molecular structure.
authors.affiliation: Department of Molecular Biology, Jilin University, Changchun, P.R. China.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Zhang
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.forename: H
authors.initials: H
authors.lastname: Zhou
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.forename: R
authors.initials: R
authors.lastname: Smith
authors.forename: J
authors.initials: J
authors.lastname: de Jersey
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000821:virology; D006868:Hydrolysis; D009842:Oligopeptides / Q000138:chemical synthesis / Q000378:metabolism; D019151:Peptide Library; D011485:Protein Binding / Q000187:drug effects; D015202:Protein Engineering / Q000379:methods; D015842:Serine Proteinase Inhibitors / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D009842:Oligopeptides; D019151:Peptide Library; D015842:Serine Proteinase Inhibitors; D002918:Chymotrypsin