Luminol luminescence induced by oxidants in antioxidant-deficient yeasts Saccharomyces cerevisiae.
Luminol chemiluminescence induced in the presence of yeast cells and yeast cell homogenates was significantly induced by exogenous oxidants (hydrogen peroxide and menadione). tert-Butyl hydroperoxide did not stimulate chemiluminescence by itself but augmented menadione-induced chemiluminescence. Comparison of yeast strains deficient in catalase, superoxide dismutase or glutathione showed that only glutathione-deficient strains showed elevated chemiluminescence in this system. These results support the idea that more reactive species than hydrogen peroxide and superoxide are critical in the induction of luminol chemiluminescence.
Jakubowski
W
W
Department of Molecular Biophysics, University of Lódź, Poland. jakubo@biol.uni.lodz.pl
Ertel
D
D
Biliński
T
T
Kedziora
J
J
Bartosz
G
G
D020642:Acatalasia; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000172:deficiency / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism*; D016877:Oxidants / Q000502:physiology*; D010084:Oxidation-Reduction; D010545:Peroxides / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000172:deficiency / Q000378:metabolism; D014812:Vitamin K / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D000975:Antioxidants; D016877:Oxidants; D010545:Peroxides; D014812:Vitamin K; D008165:Luminol; D020122:tert-Butylhydroperoxide; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione