Differential production of active oxygen species in photo-symbiotic and non-symbiotic bivalves.
We investigated the generation of active oxygen species in the bivalves, Crassostrea gigas, Fulvia mutica and Tridacna crocea in order to understand the defensive mechanisms in giant clams that allow a stable association with symbiotic zooxanthellae. C. gigas produced active oxygens, superoxide anion and nitric oxide upon stimulation by phorbol myristate acetate. F. mutica generated a little amount of superoxide anion and nitric oxide, and contained significant phenoloxidase activity which catalyzes formation of quinones. T. crocea did not generate any apparent active oxygen species or quinones. The importance of lacking rapid cytotoxic responses consisting of active oxygen species to foreign organisms in the symbiotic clam is discussed.
Nakayama
K
K
Marine Biotechnology Institute, Kamaishi Laboratories, Heita, Japan. knakayama@kamaishi.mbio.co.jp
Maruyama
T
T
D000818:Animals; D049872:Bivalvia / Q000378:metabolism* / Q000469:parasitology; D004141:Dinoflagellida / Q000502:physiology*; D008974:Mollusca / Q000378:metabolism* / Q000469:parasitology; D014442:Monophenol Monooxygenase / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D010125:Ostreidae / Q000378:metabolism* / Q000469:parasitology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism; D013559:Symbiosis
D017382:Reactive Oxygen Species; D013481:Superoxides; D009569:Nitric Oxide; D014442:Monophenol Monooxygenase