[Lymphocyte transformation test (LTT) as a model for evaluating cytokine-induced immunomodulation in renal cell carcinoma].
We investigated the immunomodulatory capacity of cytokines produced by renal cell carcinoma in vitro by analyzing their effects on mitogen-induced T-lymphocyte blast cell transformation. All of the tested 70 cell cultures, derived from 70 tumor areas in 33 patients, had immunomodulatory capacity. In addition to suppression in the lymphocyte transformation test (max. 44/70; 63%) there was also superinduction (max. 37/70; 53%). We found no significant correlation with the stage and grade of primary tumors. However, the suppression of mitogen-induced T-lymphocyte blast cell transformation was significant in multifocal tumors (0.08% TCM, P < 0.001) and non-significant in metastatic tumors. The production of the assayed cytokines IL-6, IL-10, IL-11, and TGF beta 1 was variable and there was no significant correlation to the immunomodulatory capacity of the tumors.
Steiner
T
T
Klinik und Poliklinik für Urologie, Friedrich-Schiller-Universität Jena.
Junker
U
U
Wunderlich
H
H
Knöfel
B
B
Schlichter
A
A
Schubert
J
J
D002292:Carcinoma, Renal Cell / Q000276:immunology*; D016207:Cytokines / Q000502:physiology*; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology; D007121:Immunocompetence / Q000276:immunology; D007680:Kidney Neoplasms / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D009378:Neoplasms, Multiple Primary / Q000276:immunology; D011379:Prognosis; D013601:T-Lymphocytes / Q000276:immunology*; D014407:Tumor Cells, Cultured / Q000276:immunology
D016207:Cytokines
Lymphozytentransformations-test (LTT) als Modell zur Uberprüfung der zytokinvermittelten Immunmodulation beim Nierenzellkarzinom.