Interleukin-1 expression during hyperoxic lung injury in the mouse.
An important component of the pathophysiologic response to hyperoxia (O2) is pulmonary inflammation, although the roles of specific inflammatory mediators during pulmonary O2 toxicity are not completely known. Interleukin-1 (IL-1) is an early inflammatory mediator and is sufficient to elicit many of the responses associated with acute injury. The IL-1 family comprises two bioactive proteins, IL-1alpha and IL-1beta, and their natural antagonist IL-1ra. Here we report studies of IL-1 regulation during hyperoxic lung injury in the adult mouse. When assayed by Northern blot, increases in IL-1beta mRNA were seen after 2 days of hyperoxia. In contrast, IL-1alpha mRNA was barely detectable before 4 days of hyperoxia. To further understand the cellular origin of IL-1beta expression in lungs, in situ hybridization and immunohistochemical analyses were performed. IL-1beta mRNA or protein was not detected in the lungs of unexposed animals. At 3 days, we observed the accumulation of IL-1beta transcripts in pulmonary interstitial macrophages and in a subset of neutrophils, and immunodetectable IL-1beta protein was co-localized in adjacent sections. At 4 days of exposure, IL-1beta transcripts were widespread in lung tissue, but many areas rich in IL-1beta mRNA were devoid of immunodetectable IL-1beta. However, it is not known whether increased synthesis of IL-1beta or the uncoupling of IL-1beta protein and mRNA accumulation has a role in pathophysiology of pulmonary O2 toxicity.
Piedboeuf
B
B
Unité de Recherche de Pédiatrie, Centre de Recherche du CHUL, Centre Hospitalier Universitaire de Québec, Ste-Foy, Canada. Bruno.Piedboeuf@crchul.ulaval.ca
Horowitz
S
S
Johnston
C J
CJ
Gamache
M
M
Bélanger
S
S
Poubelle
P E
PE
Welty
S E
SE
Watkins
R H
RH
D000818:Animals; D018496:Hyperoxia / Q000378:metabolism*; D017403:In Situ Hybridization; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008168:Lung / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000633:toxicity; D010477:Perfusion; D012333:RNA, Messenger / Q000378:metabolism
D007375:Interleukin-1; D012333:RNA, Messenger; D010100:Oxygen