Cell turnover parameters in small and large cell varieties of primary intestinal non-Hodgkin's lymphoma.
BACKGROUND
In contrast to primary gastric lymphoma, primary intestinal lymphoma is an uncommon condition with a poorer outcome, perhaps due to differences in its pathogenesis. In this study, the authors analyzed the roles of proliferation and apoptosis in the pathogenesis of intestinal lymphoma.


METHODS
Fifty-one cases of intestinal non-Hodgkin's lymphoma (NHL) (10 small B-cell mucosa-associated lymphoid tissue [MALT] NHLs, 12 large B-cell MALT NHLs, 18 large B-cell NHLs, 2 small T-cell NHLs, 7 large T-cell NHLs, and 2 mantle cell NHLs) were studied for the immunohistochemical expression of MIB-1 and the TUNEL assay as well as the expression of bcl-2 and p53, both of which are regulatory gene products involved in apoptosis.


RESULTS
The median proliferation index (PI) was 37.3%, and the median apoptotic index (AI) was 1.10%. The respective values of PI and AI were 5.8% and 0.06% in small B-cell MALT lymphoma, 52.8% and 0.24% in large B-cell MALT lymphoma, 58.85% and 1.36% in large B-cell lymphoma, 30.9% and 1.93% in mantle cell lymphoma, 18.13% and 1.25% in small T-cell lymphoma, and 43.4% and 1.93% in large T-cell lymphoma. In an analysis of B-cell NHL only (with mantle cell NHL excluded), proliferative and apoptotic indices were positively correlated (correlation coefficient=0.563, P < 0.001). Furthermore, high bcl-2 expression was inversely correlated with both PI and AI. Expression of p53 was observed in 8 cases (1 small cell lymphoma and 7 large cell lymphomas).


CONCLUSIONS
Small cell lymphomas had low AI and PI values, whereas large cell lymphomas had high AI and PI values. Apoptosis and proliferation were positively correlated, and higher expression of bcl-2 was associated with lower rates of apoptosis.
Gisbertz
I A
IA
Department of Internal Medicine, University Hospital Maastricht, The Netherlands.
Schouten
H C
HC
Bot
F J
FJ
Arends
J W
JW
D017209:Apoptosis; D002455:Cell Division; D016158:Genes, p53; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis
D019253:Proto-Oncogene Proteins c-bcl-2