Comparison of mitochondrial respiratory chain enzyme activities in rodent astrocytes and neurones and a human astrocytoma cell line.
This study has found that mitochondrial NADH-CoQ1 reductase (complex I) activity is significantly lower in C57 mice astrocytes compared with Wistar and Sprague-Dawley rat astrocytes, and a human astrocytoma cell line. In addition, complex I activity is 4-fold greater in Sprague-Dawley neurones when compared to Wistar or C57 neurones. These findings have important implications for mitochondrial studies involving rodent or human cell line systems, and in particular, indicate the importance of choosing an appropriate model when investigating the mitochondrial respiratory chain.
Stewart
V C
VC
Department of Neurochemistry, Institute of Neurology, London, UK. v.stewart@ion.ucl.ac.uk
Land
J M
JM
Clark
J B
JB
Heales
S J
SJ
D000818:Animals; D001253:Astrocytes / Q000201:enzymology*; D001254:Astrocytoma / Q000201:enzymology / Q000473:pathology*; D001932:Brain Neoplasms / Q000201:enzymology / Q000473:pathology*; D002478:Cells, Cultured; D002950:Citrate (si)-Synthase / Q000032:analysis; D003574:Cytochrome c Group / Q000032:analysis; D004579:Electron Transport; D042967:Electron Transport Complex I; D042963:Electron Transport Complex II; D014450:Electron Transport Complex III / Q000032:analysis; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000032:analysis; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000032:analysis*; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity; D013385:Succinate Dehydrogenase / Q000032:analysis; D014407:Tumor Cells, Cultured / Q000201:enzymology
D003574:Cytochrome c Group; D009097:Multienzyme Complexes; D009419:Nerve Tissue Proteins; D010088:Oxidoreductases; D042963:Electron Transport Complex II; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D016660:NAD(P)H Dehydrogenase (Quinone); D002950:Citrate (si)-Synthase; D042967:Electron Transport Complex I; D014450:Electron Transport Complex III