Establishment and characterization of bone marrow stroma-dependent leukemia cell line, HB-1.
Radiation-induced M361 leukemia bearing mice (M361 mice) show characteristics of acute myelogenous leukemia (AML) with granulocytosis. Granulocyte-macrophage colony forming unit (CFU-GM) increased in the bone marrow and spleen and high activity of colony stimulating factor (CSF) was found in the sera of M361 mice. A cell line (HB-1) was established from the spleen cells of M361 mice. Injection of HB-1 cells induced a similar leukemic response as M361 in syngeneic mice. HB-1 cells did not survive in the suspension culture, but proliferated when cultured on hemopoietic supportive stromal cells (MS-10). HB-1 cells appear to be strictly dependent on the hemopoietic supportive bone marrow stroma, which would provide a useful model for the study of cell-cell interactions between hemopoietic cells and marrow stromal cells.
Sawada
H
H
Department of Internal Medicine, Kokura Memorial Hospital, Kitakyushu, Japan.
Sugimoto
K
K
Jiang
H
H
Tsurumaki
Y
Y
Breckon
G
G
Lorimore
S A
SA
Wright
E G
EG
Mori
K J
KJ
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000473:pathology; D002450:Cell Communication; D002470:Cell Survival; D018920:Coculture Techniques; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D051379:Mice; D017154:Stromal Cells / Q000473:pathology*; D014407:Tumor Cells, Cultured