A novel importin alpha from rice, a component involved in the process of nuclear protein transport.
In eukaryotes, nuclear proteins that are transported into nuclei have nuclear localization signals (NLSs), which are recognized by proteins called importin alpha. We isolated a rice cDNA, #61L, and the corresponding gene that encodes a protein, which shows significant homology to the importin alpha. Although the encoded protein had only 23-27% amino acid identity to the importin alphas from various organisms including plants, the fusion protein with glutathione S-transferase showed a specific binding activity to the NLS of SV40 T-antigen. These results suggest that the rice #61L protein is a novel importin alpha in plants.
Iwasaki
T
T
Department of Biology, Faculty of Science, Niigata University, Ikarashi, Japan. tiwasaki@sc.niigata-u.ac.jp
Matsuki
R
R
Shoji
K
K
Sanmiya
K
K
Miyao
M
M
Yamamoto
N
N
D000595:Amino Acid Sequence; D000818:Animals; D000957:Antigens, Viral, Tumor / Q000737:chemistry / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism; D001665:Binding Sites; D002467:Cell Nucleus / Q000502:physiology; D017343:Genes, Plant; D005982:Glutathione Transferase / Q000096:biosynthesis; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics* / Q000378:metabolism*; D010802:Phylogeny; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013539:Simian virus 40 / Q000378:metabolism; D014981:Xenopus; D028901:alpha Karyopherins
D000957:Antigens, Viral, Tumor; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins; D028901:alpha Karyopherins; D005982:Glutathione Transferase