N-hydroxysuccinimide ester labeling 5'-aminoalkyl DNA oligomers: reaction conditions and purification.
Difficulties were encountered in labeling 5'-aminoalkyl DNA oligomers with glycolketo electrophore N-hydroxysuccinimide esters in aqueous sodium bicarbonate (a common base for this purpose), followed by C18-silica reversed-phase high-performance liquid chromatography (HPLC) to achieve purification. The electrophore-labeled oligomers were not separated readily either from the hydrolyzed electrophore or from the starting oligomer. This problem was overcome by conducting the reaction with triethylamine as a base, organic washing the reaction mixtures after evaporation, and separating on a C18-poly(styrene-divinylbenzene) HPLC packing.
Wang
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Z
Department of Pharmaceutical Sciences, Bouvé College of Pharmacy and Health Professions, Barnett Institute, Northeastern University, Boston, MA 02115, USA.
Bian
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Qian
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Giese
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RW
D000097:Acetonitriles; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification*; D015342:DNA Probes; D004952:Esters; D006868:Hydrolysis; D015394:Molecular Structure; D017693:Sodium Bicarbonate; D013388:Succinimides
D000097:Acetonitriles; D015342:DNA Probes; D004952:Esters; D013388:Succinimides; D017693:Sodium Bicarbonate; D004247:DNA; C001426:N-hydroxysuccinimide; C032159:acetonitrile