Ectopic bone formation induced by biodegradable hydrogels incorporating bone morphogenetic protein.
Biodegradable hydrogels were prepared from gelatin by glutaraldehyde cross-linking for release matrix of recombinant human bone morphogenetic protein-2 (BMP-2). BMP-2 solution was impregnated into the dried hydrogels to prepare BMP-2-incorporating gelatin hydrogels. In the in vitro study, enhanced retention of BMP-2 was observed from the BMP-2-incorporating gelatin hydrogels after an initial burst of BMP-2 incorporated initially in the hydrogel. Following subcutaneous implantation of (125)I-labeled BMP-2-incorporating gelatin hydrogels in the back of mice, the radioactivity remaining in the hydrogels was measured to estimate the in vivo release profile of BMP-2. It was found that BMP-2 was retained in the hydrogels for longer than 30 days, whereas 99% of BMP-2 injected in the solution form was cleared from the injected site within one day, completely disappearing within 3 days. Ectopic bone formation studies demonstrated that BMP-2-incorporating gelatin hydrogels exhibited a more potent ability for bone induction than solution injection of BMP-2. This finding indicates that enhanced retention of BMP-2 is promotes its ability to induce ectopic bone formation.
Yamamoto
M
M
Research Center for Biomedical Engineering, Kyoto University, Japan.
Tabata
Y
Y
Ikada
Y
Y
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D001842:Bone and Bones; D002828:Choristoma / Q000209:etiology; D003432:Cross-Linking Reagents / Q000737:chemistry; D004343:Drug Implants; D005260:Female; D005780:Gelatin / Q000737:chemistry; D005782:Gels / Q000737:chemistry*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D011994:Recombinant Proteins / Q000494:pharmacology; D012871:Skin Diseases / Q000209:etiology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
C525718:BMP2 protein, human; D001672:Biocompatible Materials; C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D003432:Cross-Linking Reagents; D004343:Drug Implants; D005782:Gels; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D005780:Gelatin; D005976:Glutaral