title: Thermostable neutral protease resembling thermolysin derived from Bacillus brevis MIB001.
abstract: A microbe producing a protease with strong thermostability that was released extracellularly was isolated from soil. The isolate, MIB001, grew at from 15 to 51 degrees C and pH 5.1-8.8 and was tentatively identified as a strain of Bacillus brevis. Rabbit antisera raised against a pure preparation of the protease did not cross-react with thermolysin or neutral metalloprotease from Bacillus stearothermophilus KP1236.
authors.affiliation: Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Hyogo, Japan. takii@mwu.mukogawa-u.ac.jp
authors.forename: Y
authors.initials: Y
authors.lastname: Takii
authors.forename: Y
authors.initials: Y
authors.lastname: Urata
authors.forename: N
authors.initials: N
authors.lastname: Ueno
mesh_terms: D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000302:isolation & purification*; D010450:Endopeptidases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D012988:Soil Microbiology; D013696:Temperature; D013820:Thermolysin / Q000378:metabolism*
chemical_list: D010450:Endopeptidases; D013820:Thermolysin