Transovarian transmission of a foreign gene in the silkworm, Bombyx mori, by Autographa californica nuclear polyhedrosis virus.
We introduced a firefly luciferase gene, expressed under control of Drosophila heat shock protein gene promoter, into Autographa californica nuclear polyhedrosis virus (AcNPV). When the 5th instar larvae of the silkworm, Bombyx mori, were inoculated with the recombinant virus, luciferase activities were detected in the virus-infected larvae and pupae, and in the newly hatched larvae of the next generation. PCR amplification and Southern blot hybridization analysis demonstrated that the luciferase gene was transmitted through at least the F2 generation. In addition, the V-cathepsin gene, encoding a cysteine protease of AcNPV, was also detected in the DNA of all individuals of the F2 generation. These results show that AcNPV can be utilized as vector for the transovarian transmission of foreign genes in the silkworm.
Mori
H
H
Department of Applied Biology, Faculty of Textile Science, Kyoto Institute of Technology, Japan.
Yamao
M
M
Nakazawa
H
H
Sugahara
Y
Y
Shirai
N
N
Matsubara
F
F
Sumida
M
M
Imamura
T
T
D000818:Animals; D030801:Animals, Genetically Modified; D015139:Blotting, Southern; D012831:Bombyx / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D005822:Genetic Vectors; D006360:Heat-Shock Proteins / Q000235:genetics; D007814:Larva / Q000378:metabolism; D008156:Luciferases / Q000235:genetics; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011679:Pupa / Q000378:metabolism; D011993:Recombinant Fusion Proteins; D014162:Transfection
D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D008156:Luciferases