Recoverin and hippocalcin distribution in the lamprey (Lampreta fluviatilis) retina.
Recoverin is a calcium-sensing protein which is involved in the transduction of light in vertebrate photoreceptors. It is also detected in other retina cell types in which its function is not yet elucidated, and is an autoantigen in a cancer-associated degenerative disease of the retina. Recently, hippocalcin, an homologous protein of recoverin, belonging to the same family of fatty acylated EF-hand calcium binding proteins was described in mammals. The immunohistochemical studies presented in this paper demonstrate, that, in the retina of the lamprey, an Agnathan considered the living ancestor of actual jawed vertebrates, recoverin was present in all photoreceptors and, to a lesser extent in subpopulations of amacrine and ganglion cells whereas hippocalcin was detected in numerous amacrine and ganglion cells and in the inner segments of long photoreceptors. The existence of these calcium-binding proteins shows that they have a high degree of conservation during evolution. Their presence in the same cells that in jawed vertebrates (photoreceptors and ganglion cells for recoverin; amacrine and ganglion cells for hippocalcin) suggests that some retinal functions are well conserved but because they were also found in different cell types than in other species (amacrine for recoverin; photoreceptors for hippocalcin), they may have functions more specific to the lamprey retina.
Dalil-Thiney
N
N
Laboratoire de NeuroCytologie Oculaire, INSERM U450/XR86, Paris, France.
Bastianelli
E
E
Pochet
R
R
Repérant
J
J
Versaux-Botteri
C
C
D000818:Animals; D002118:Calcium / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000032:analysis*; D019143:Evolution, Molecular; D005136:Eye Proteins / Q000032:analysis*; D051597:Hippocalcin; D007798:Lampreys / Q000378:metabolism*; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D010786:Photoreceptor Cells / Q000737:chemistry*; D051603:Recoverin; D012160:Retina / Q000737:chemistry*; D012165:Retinal Ganglion Cells / Q000737:chemistry; D012374:Rod Cell Outer Segment / Q000737:chemistry; D013045:Species Specificity; D013569:Synapses / Q000737:chemistry; D014714:Vertebrates / Q000378:metabolism
D002135:Calcium-Binding Proteins; D005136:Eye Proteins; D008074:Lipoproteins; D009419:Nerve Tissue Proteins; D051603:Recoverin; D051597:Hippocalcin; D002118:Calcium