Effect of culture medium on the in vitro secretion activity of prothoracic glands from Pseudaletia separata.
The prothoracic glands (PGs) taken from the last instar of the common armyworm, Pseudaletia separata, were cultured in various media for the purpose of finding a suitable medium for relatively long-term culture of PGs. Among the tested culture media, MGM-450 medium without serum was the best to maintain PG cells viable for relatively long periods, and to continue to secrete ecdysteroids. Secretion of ecdysteroid by the PG in vitro became marked when the PG was taken from last instar larvae older than 2 days after the last molt. PGs cultured in any of the media secreted ecdysteroid only within the first 2 h after placing them in culture, however, in the MGM-450 medium, the PGs secreted ecdysteroid even after 5 days of culture.
Komiya
K
K
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Agui
N
N
Mitsuhashi
J
J
D000818:Animals; D002470:Cell Survival; D003470:Culture Media; D026461:Ecdysteroids; D004702:Endocrine Glands / Q000378:metabolism / Q000502:physiology*; D007700:Kinetics; D007814:Larva; D009036:Moths / Q000502:physiology*; D009924:Organ Culture Techniques; D013256:Steroids / Q000096:biosynthesis*
D003470:Culture Media; D026461:Ecdysteroids; D013256:Steroids