title: [The secretion and receptor gene of insulin-like growth factor-I quantitative expression in ovarian stroma in polycystic ovarian syndrome patients].
abstract: OBJECTIVE
  To explore varying different degrees of the action of insulin-like growth factor-I (IGF-I) and its receptor gene quantitative expression in the ovarian stroma of women with obese and nonobese polycystic ovarian syndrome (PCOS).
  
  
  METHODS
  The expression of IGF-I receptor gene in the ovarian stroma from 35 cases of PCOS (obese: 15 cases; nonobese: 20 cases) and 20 cases of control were quantitatively determined by reverse transcript polymerase chain reaction (RT-PCR) combined with beta(2) micro globlin (beta(2) MG) gene as inner-control to amplificate sychronously. The grey value ratio of them were determined. The PCR-amplified cDNA fragments were put in restriction analysis (DdeI).
  
  
  RESULTS
  The expression of IGF-I receptor in PCOS ovary stroma was significantly greater than in the control group (P < 0.001). The nonobese group presents more enhancement than obese group in IGF-I receptor gene expression. The restrictive fragments were same between the PCOS and the control group.
  
  
  CONCLUSIONS
  The expression of IGF-I receptor in PCOS ovarian stroma is abnormally excessive and the nonobese PCOS have more potential action of IGF-I system in ovary stroma.
authors.affiliation: First Affiliated Hospital of Sun Yat-Sen University of Medical Science, Guangzhou.
authors.forename: X
authors.initials: X
authors.lastname: Liang
authors.forename: G
authors.initials: G
authors.lastname: Zhuang
authors.forename: Q
authors.initials: Q
authors.lastname: Fang
mesh_terms: D000328:Adult; D005260:Female; D015870:Gene Expression; D006801:Humans; D009765:Obesity / Q000150:complications; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D017526:Receptor, IGF Type 1