title: Changing nucleosome positions in vivo through modification of the DNA rotational information.
abstract: The effects of the rotational information of DNA in determining the in vivo localization of nucleosomal core particles (ncps) have been studied in the Saccharomyces cerevisiae 5 S rRNA repeat gene. The distribution of the phased series of flexibility signals present in this DNA has been altered by inserting in its centre a 25 bp tract. The effects of such alteration on the in vivo distribution of the helically phased, alternatively located ncps have been determined relative to a reference 21 bp insertion mutant. The results show that the answers provided in vitro and in vivo by the yeast 5 S rRNA gene sequence to specific modifications of the DNA rotational frame are similar, thus pointing to the relevance of DNA rotational information in vivo.
authors.affiliation: Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, P. le Aldo Moro, 5 00185 Roma, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Di Marcotullio
authors.forename: M
authors.initials: M
authors.lastname: Buttinelli
authors.forename: G
authors.initials: G
authors.lastname: Costanzo
authors.forename: E
authors.initials: E
authors.lastname: Di Mauro
authors.forename: R
authors.initials: R
authors.lastname: Negri
mesh_terms: D015825:Chromosomes, Fungal; D004262:DNA Restriction Enzymes / Q000235:genetics; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D009690:Nucleic Acid Conformation; D009707:Nucleosomes / Q000235:genetics / Q000648:ultrastructure*; D010957:Plasmids; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004271:DNA, Fungal; D009707:Nucleosomes; D012341:RNA, Ribosomal, 5S; D004262:DNA Restriction Enzymes