Growth hormone reduces mortality and bacterial translocation in irradiated rats.
Growth hormone stimulates the growth of intestinal mucosa and may reduce the severity of injury caused by radiation. Male Wistar rats underwent abdominal irradiation (12 Gy) and were treated with either human growth hormone (hGH) or saline, and sacrificed at day 4 or 7 post-irradiation. Bacterial translocation, and the ileal mucosal thickness, proliferation, and disaccharidase activity were assessed. Mortality was 65% in irradiated animals, whereas hGH caused a decrement (29%, p < 0.05). Bacterial translocation was also reduced by hGH (p < 0.05). Treating irradiated rats with hGH prevented body weight loss (p < 0.05). Mucosal thickness increased faster in irradiated hGH-treated animals. The proliferative index showed an increment in hGH-treated animals (p < 0.05). Giving hGH to irradiated rats prevented decrease in sucrose activity, and increment in lactase activity. In conclusion, giving hGH to irradiated rats promotes the adaptative process of the intestine and acute radiation-related negative effects, including mortality, bacterial translocation, and weight loss.
Gómez-de-Segura
I A
IA
Department of Experimental Surgery, La Paz Hospital, Madrid, Spain.
Prieto
I
I
Grande
A G
AG
García
P
P
Guerra
A
A
Mendez
J
J
De Miguel
E
E
D000005:Abdomen / Q000528:radiation effects*; D000818:Animals; D018988:Bacterial Translocation / Q000187:drug effects* / Q000528:radiation effects; D002455:Cell Division; D019382:Human Growth Hormone / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000382:microbiology / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D011833:Radiation Injuries, Experimental / Q000401:mortality / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D018809:Proliferating Cell Nuclear Antigen; D019382:Human Growth Hormone