[The approach of cloned method in a human retinoblastoma cell line SO-Rb50].
OBJECTIVE
In order to obtain the same characteristic tumor cells in genetical and biological aspect, the cloned human retinoblastoma cell line SO-Rb50 established in our department is reported in this paper.


METHODS
SO-Rb50 cells were cloned in multi-well plates.


RESULTS
Non-rosette Rb cells were successfully cloned while rosette Rb cells failed.


CONCLUSIONS
The result suggested that high differentiated Rb cells were difficult to be cloned.
Zheng
J
J
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
Feng
G
G
Li
Y
Y
Yi
Y
Y
D002999:Clone Cells; D006801:Humans; D019572:Retinal Neoplasms / Q000473:pathology*; D012175:Retinoblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured