Partial respecification of nasotemporal polarity in double-temporal chick and chimeric chick-quail eyes.
In chick embryos, naso-temporal polarity of the retina becomes established before Hamburger-Hamilton stage 10. To examine the plasticity of the early eye anlage, double-temporal eyes were made using stage 10-11 (E1.5) chick embryos and stage 8-9 quail embryos. In vivo and in vitro experiments revealed that these double-temporal compound eyes were not completely temporal but nasal in a large peripheral part of the graft. Four hours after transplantation, the nasal-specific fork head transcription factor CBF1 was not expressed in double-temporal eyes but was clearly detectable 24 h later. This suggests that in the peripheral part of the graft, temporal positional values were changed into nasal positional values by a respecification process.
Mueller
B K
BK
Max-Planck-Institut für Entwicklungsbiologie, Spemannstrasse 35, D-72076 Tübingen, Germany. bemue@mpib-tuebingen.mpg.de
Dütting
D
D
Haase
A
A
Feucht
A
A
Macchi
P
P
D000818:Animals; D001369:Axons / Q000648:ultrastructure; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D002642:Chick Embryo / Q000502:physiology*; D002678:Chimera / Q000196:embryology*; D003370:Coturnix / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D012160:Retina / Q000196:embryology*; D013045:Species Specificity; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D014183:Transplantation, Heterologous; D014795:Visual Pathways / Q000502:physiology
D014157:Transcription Factors