Intestinal adaptation after extensive small bowel resection: differential changes in growth and insulin-like growth factor system messenger ribonucleic acids in jejunum and ileum.
The distal small bowel exhibits greater adaptive growth than proximal segments after partial small intestine resection. To explore this process, we evaluated adaptive cellularity, intestinal insulin-like growth factor (IGF) system messenger RNA (mRNA) transcripts, and effects of recombinant IGF-I treatment in jejunum and ileum of adult rats. Gastrostomy-fed animals underwent 80% jejuno-ileal resection or intestinal transection and reanastomosis without resection, followed by infusion of human recombinant IGF-I (2.4 mg/kgXday) or vehicle. After 7 days, resected rats demonstrated modest adaptive growth in jejunum and marked cell proliferation in ileum. Resection increased IGF-I mRNA in both jejunum (183%) and ileum (249%) and up-regulated IGFBP-4 mRNA levels in both tissues. IGFBP-3 mRNA fell significantly in ileum after resection. IGF-I infusion modestly increased ileal cellularity after resection, but had no effect in jejunum. IGF-I markedly increased IGFBP-3 mRNA levels in jejunum after both transection and resection. These data confirm that bowel resection induces greater adaptive growth in ileum than jejunum. IGF-I administration modestly increases ileal, but not jejunal, growth after resection. Increased levels of intestinal IGF-I and IGFBP-4 mRNA suggest roles for IGF-I and IGFBP-4 in mediating small bowel adaptation. Higher levels of jejunal IGFBP-3 mRNA may be related to limited jejunal vs. ileal growth after extensive jejuno-ileal resection.
Ziegler
T R
TR
Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
Mantell
M P
MP
Chow
J C
JC
Rombeau
J L
JL
Smith
R J
RJ
D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D001835:Body Weight; D004247:DNA / Q000378:metabolism; D007082:Ileum / Q000378:metabolism; D007421:Intestine, Small / Q000254:growth & development / Q000503:physiopathology* / Q000601:surgery*; D007583:Jejunum / Q000378:metabolism; D008297:Male; D009929:Organ Size; D011184:Postoperative Period; D011506:Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013002:Somatomedins / Q000235:genetics* / Q000378:metabolism
D011506:Proteins; D012333:RNA, Messenger; D013002:Somatomedins; D004247:DNA