Biochemical and genetic characterization of three molybdenum cofactor hydroxylases in Arabidopsis thaliana.
Aldehyde oxidases and xanthine dehydrogenases/oxidases belong to the molybdenum cofactor dependent hydroxylase class of enzymes. Zymograms show that Arabidopsis thaliana has at least three different aldehyde oxidases and one xanthine oxidase. Three different cDNA clones encoding putative aldehyde oxidases (AtAO1, 2, 3) were isolated. An aldehyde oxidase is the last step in abscisic acid (ABA) biosynthesis. AtAO1 is mainly expressed in seeds and roots which might reflect that it is involved in ABA biosynthesis.
Hoff
T
T
Department of Plant Physiology, University of Copenhagen, Oester Farimagsgade 2A, 1353 Copenhagen K, Denmark. hoff@biobase.dk
Frandsen
G I
GI
Rocher
A
A
Mundy
J
J
D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018744:DNA, Plant; D006801:Humans; D008969:Molecular Sequence Data; D008982:Molybdenum; D017386:Sequence Homology, Amino Acid; D014968:Xanthine Dehydrogenase / Q000378:metabolism
D029681:Arabidopsis Proteins; D018744:DNA, Plant; D008982:Molybdenum; D014968:Xanthine Dehydrogenase; D000445:Aldehyde Oxidoreductases; C467964:AAO1 protein, Arabidopsis; D042931:Aldehyde Oxidase