Two- and three-dimensional high-resolution imaging of the human oviduct with optical coherence tomography.
OBJECTIVE
To evaluate the feasibility of optical coherence tomography, a new method of micron-scale imaging, for high-resolution assessment of the oviduct. Optical coherence tomography is analogous to ultrasound except that it measures the backreflection of infrared light rather than acoustical waves.


METHODS
The ampulla of a human fallopian tube was imaged in vitro using optical coherence tomography. Images were generated in 2 and 3 dimensions.


METHODS
University.


METHODS
Samples were obtained from women who had undergone hysterectomy for leiomyomatosis.


METHODS
None


METHODS
The ability to perform imaging on a micron scale, which is a level of resolution higher than that of any currently available clinical technology.


RESULTS
Two- and three-dimensional data sets of the reflectance of a human fallopian tube were acquired. A volume of 5 x 5 x 2.5 mm (length x width x depth) was scanned. The axial resolution was 11 microm, and the lateral resolution at the focus was 20 microm. The data sets showed detailed structures of the fallopian tube.


CONCLUSIONS
Our ability to obtain micron-scale two- and three-dimensional images of an in vitro oviduct suggests that it may be possible to identify and surgically treat tubal causes of infertility.
Herrmann
J M
JM
Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge 02139, USA.
Brezinski
M E
ME
Bouma
B E
BE
Boppart
S A
SA
Pitris
C
C
Southern
J F
JF
Fujimoto
J G
JG
D003952:Diagnostic Imaging; D004724:Endoscopy; D005187:Fallopian Tubes / Q000033:anatomy & histology* / Q000000981:diagnostic imaging; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007259:Infrared Rays; D014054:Tomography; D014463:Ultrasonography