Duchenne muscular dystrophy: a model for studying the contribution of muscle to energy and protein metabolism.
Duchenne muscular dystrophy (DMD) is associated with a dramatic muscle mass loss. We hypothesized that DMD would be associated with significant changes in both energy and protein metabolism. We studied the resting energy expenditure (REE) in DMD and control children using indirect calorimetry, and their protein metabolism using an intravenous infusion of leucine and glutamine labeled with stable isotopes. In spite of a 75% muscle mass loss in the DMD children, the REE only decreased by 10%. DMD was associated with increased leucine oxidation but neither protein degradation nor protein synthesis were different from that of the controls. In contrast, whole body turnover of glutamine, an amino acid mainly synthesized in the muscle, was significantly decreased. These studies emphasized the quantitatively poor contribution of muscle to energy and protein metabolism in children. The qualitative impact of muscle mass loss on amino acid metabolism (glutamine) offers a fascinating field of research for the next few years and has therapeutic potential.
Hankard
R
R
Centre d'investigation clinique, Hôpital Robert-Debré, Paris, France. regis.hankard@rdb.ap-hop-paris.fr
D001481:Basal Metabolism; D002153:Calorimetry, Indirect; D002648:Child; D004734:Energy Metabolism; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D007930:Leucine / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D009136:Muscular Dystrophies / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism*
D011506:Proteins; D005973:Glutamine; D007930:Leucine