The role of P-selectin, sialyl Lewis X and sulfatide in myocardial ischemia and reperfusion injury.
The role of P-selectin and the ligands of selectins such as sialyl Lewis X and sulfatide was studied in a myocardial ischemia and reperfusion injury model. Anesthetized rabbits underwent the occlusion of coronary artery (30 min) followed by reperfusion (5 h). The inhibitory effect on myocardial ischemia and reperfusion injury was examined with infarct size normalized by area-at-risk. Intravenous administration of an anti-P-selectin monoclonal antibody, PB1.3 (2 mg/kg), reduced infarct size by 38%. Similarly, the administration of sialyl Lewis X-oligosaccharide (10 mg/kg) reduced infarct size by 53% significantly. Finally, the infarct size was significantly reduced bv 39% in sulfatide-treated group (10 mg/kg). These results suggest that P-selectin plays an important role in myocardial ischemia and reperfusion injury and that the ligands of selectins, such as sialyl Lewis X-oligosaccharide and sulfatide, have cardioprotective effect on myocardial ischemia and reperfusion injury.
Yamada
K
K
Sumitomo Pharmaceuticals, Research Center, Osaka, Japan.
Tojo
S J
SJ
Hayashi
M
M
Morooka
S
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D007958:Leukocyte Count; D008024:Ligands; D008297:Male; D017202:Myocardial Ischemia / Q000188:drug therapy* / Q000473:pathology; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy* / Q000473:pathology; D009844:Oligosaccharides / Q000627:therapeutic use*; D019007:P-Selectin / Q000276:immunology / Q000502:physiology*; D011817:Rabbits; D000080506:Sialyl Lewis X Antigen; D013433:Sulfoglycosphingolipids / Q000627:therapeutic use*
D000911:Antibodies, Monoclonal; D008024:Ligands; D009844:Oligosaccharides; D019007:P-Selectin; D000080506:Sialyl Lewis X Antigen; D013433:Sulfoglycosphingolipids