[Behavioral evaluation of the unilateral lesion model in rats using 6-hydroxydopamine. Correlation between the rotations induced by D-amphetamine, apomorphine and the manual dexterity test].
BACKGROUND
Evaluation of rotatory activity induced by dopaminergic agonists is the most widely used test of conduct for the measurement of dopaminergic depletion of a unilateral lesion of the striatonigral pathway caused by 6-hydroxydopamine (6-OHDA) in rats, since it is quantitatively related to the extension of the dopaminergic denervation.


OBJECTIVE
The objective of this study was to evaluate, from different angles, the changes in conduct seen in the model of unilateral lesion with 6-OHDA and to establish correlation with the rotation induced by D-amphetamine and by apomorphine and the ladder test.


METHODS
Male Wistar rats were used. Lesions were produced in the SNpc by stereotactic injection of 6-OHDA into the right hemisphere and the effectiveness of the lesions was studied using the rotary conduct induced by D-amphetamine and apomorphine. The motor ability of the front legs was measured by the ladder test, carried out under standard and forced conditions.


RESULTS
All the animals with lesions had difficulty in reaching food with both legs, although the most pronounced deficit was in the leg contralateral to the lesion. The ladder test correlated better with rotatory activity induced by apomorphine than by D-amphetamine.


CONCLUSIONS
The animals with most dopamine loss showed most deficient use of their front legs.
Pavón
N
N
Centro Internacional de Restauración Neurológica, CIREN, La Habana, Cuba.
Vidal
L
L
Alvarez
P
P
Blanco
L
L
Torres
A
A
Rodríguez
A
A
Macías
R
R
D000818:Animals; D001058:Apomorphine / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects*; D003342:Corpus Striatum / Q000187:drug effects* / Q000503:physiopathology; D003913:Dextroamphetamine / Q000494:pharmacology*; D004292:Dominance, Cerebral; D004298:Dopamine / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D005247:Feeding Behavior / Q000187:drug effects; D005528:Foot / Q000503:physiopathology*; D005552:Forelimb / Q000503:physiopathology; D008297:Male; D009043:Motor Activity / Q000187:drug effects*; D016627:Oxidopamine / Q000633:toxicity*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000503:physiopathology*; D011597:Psychomotor Performance / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013238:Stereotaxic Techniques; D013239:Stereotyped Behavior / Q000187:drug effects*; D013378:Substantia Nigra / Q000187:drug effects* / Q000503:physiopathology; D013563:Sympathectomy, Chemical; D013565:Sympatholytics / Q000633:toxicity*
D013565:Sympatholytics; D016627:Oxidopamine; D001058:Apomorphine; D003913:Dextroamphetamine; D004298:Dopamine
Evaluación conductual del modelo de lesión unilateral en ratas con 6-hidroxidopamina. Correlación entre las rotaciones inducidas por D-anfetamina, apomorfina y la prueba de habilidades manuales.