Mammalian and Drosophila dachshund genes are related to the Ski proto-oncogene and are expressed in eye and limb.
We have isolated mammalian homologues of the Drosophila dachshund gene. Two domains of high conservation, one of which contains an alpha-helical, coiled-coil motif, show similarity to the Ski family of genes. We therefore propose that Dachshund belongs to a superfamily including these genes. Mouse Dachshund (Dach) is expressed in the eye and limb, structures affected by the Drosophila loss-of-function mutant, and rib primordia, CNS and genital eminence. Pax6 and Dach show overlapping but non-identical expression patterns. Dach expression is unaffected in smalleye mouse brain, indicating that Pax6 is not directly activating Dach. In Drosophila eye development dachshund is a component of an interacting network of proteins. Genes homologous to many of these exist in mammals; Dach joins this expanding group.
Hammond
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KL
MRC-Human Genetics Unit, Western General Hospital, Crewe Rd, Edinburgh EH4 2XU, UK.
Hanson
I M
IM
Brown
A G
AG
Lettice
L A
LA
Hill
R E
RE
D000595:Amino Acid Sequence; D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D029721:Drosophila Proteins / Q000096:biosynthesis / Q000235:genetics*; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D020224:Expressed Sequence Tags; D005121:Extremities / Q000196:embryology*; D005123:Eye / Q000196:embryology* / Q000378:metabolism; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005326:Fetal Proteins / Q000096:biosynthesis / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D005835:Genitalia / Q000196:embryology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D007866:Leg / Q000196:embryology; D051379:Mice; D008817:Mice, Mutant Strains; D008969:Molecular Sequence Data; D009024:Morphogenesis; D005810:Multigene Family; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D017434:Protein Structure, Tertiary; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011519:Proto-Oncogenes; D012097:Repressor Proteins; D012272:Ribs / Q000196:embryology / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D029742:Caenorhabditis elegans Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D005136:Eye Proteins; D005326:Fetal Proteins; D018398:Homeodomain Proteins; C000715470:MAS1 protein, human; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D000071841:PAX6 Transcription Factor; C000605730:PAX6 protein, human; D051761:Paired Box Transcription Factors; C000605732:Pax6 protein, mouse; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; C512653:Ski protein, mouse; C103968:dac protein, Drosophila; C060614:SKI protein, human