title: Prognostic significance of DNA ploidy and S-phase fraction in malignant serous cystadenocarcinoma of the ovary.
abstract: OBJECTIVE
  To determine the prognostic value of DNA ploidy and S-phase fraction in malignant serous cystadenocarcinoma of the ovary.
  
  
  METHODS
  Flow cytometric analysis of nuclear DNA content was performed on paraffin-embedded tissues of 31 serous cystadenocarcinomas of the ovary. DNA ploidy, DNA index and S-phase fraction were determined without knowing the final outcome in the patients.
  
  
  RESULTS
  Histograms in 14 (46%) cases were aneuploid, whereas 17 (54%) were diploid. Aneuploidy was more frequent at the advanced stages of the disease as well as in the poorly differentiated histologic types. However, ploidy had a prognostic influence: both the disease-free survival and overall survival were better in diploid than aneuploid tumors at early and advanced stages. S-phase fraction (SPF) could be correctly assessed in 19 cases (62%) and was > 15% in 21% (4 cases). The survival of those patients, however, was not significantly different from that of those with SPF < 15%.
  
  
  CONCLUSIONS
  If used as an adjunct, DNA ploidy is of value in predicting tumor behavior, response to chemotherapy and disease recurrence.
authors.affiliation: Department of Cytology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: N
authors.initials: N
authors.lastname: Bakshi
authors.forename: A
authors.initials: A
authors.lastname: Rajwanshi
authors.forename: F
authors.initials: F
authors.lastname: Patel
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
mesh_terms: D000328:Adult; D018284:Cystadenocarcinoma, Serous / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D004273:DNA, Neoplasm; D005260:Female; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000473:pathology; D011003:Ploidies; D011379:Prognosis; D016196:S Phase / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm