Identification of two forms of the gamma subunit of G protein, gamma10 and gamma11, in bovine lung and their tissue distribution in the rat.
Two forms of the gamma subunit of G protein were purified from bovine lung, and were identified as gamma10 and gamma11 by analyses of partial amino acid sequences and reactivity with specific antibodies. The N-terminal amino acid residue of gamma11 was an unmodified Pro2, and the purified gamma11 was freed from beta even under non-denaturing conditions. Western blots with specific antibodies against gamma10 and gamma11 showed that both gamma subunits are present in a variety of tissues in the rat, with a particular abundance of gamma11 in the platelets.
Morishita
R
R
Department of Biochemistry, Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan.
Ueda
H
H
Kato
K
K
Asano
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D019204:GTP-Binding Proteins / Q000032:analysis*; D008168:Lung / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D014018:Tissue Distribution
D019204:GTP-Binding Proteins