DNA optical sensor: a rapid method for the detection of DNA hybridization.
A DNA optical sensor system is proposed based on the combination of sandwich solution hybridization, magnetic bead capture, flow injection and chemiluminescence for rapid detection of DNA hybridization. Bacterial alkaline phosphatase (phoA) gene and Hepatitis B virus (HBV) DNA were used as target DNA. A biotinylated DNA probe was used to capture the target gene onto the streptavidin-coated magnetic beads and a calf intestine alkaline phosphatase (CAP)-labelled DNA probe was used for subsequent enzymatic chemiluminescence detection. The detection cycle was less than 30 min, excluding the DNA hybridization time, which was about 100 min. Both the phoA gene and HBV DNA could be detected at picogramme or femtomole level. No response signal was obtained when target DNA did not exist in the sample. Successive sample detection could be made by removing the magnetic field and a washing step.
Chen
X
X
Wuhan Institute of Virology, Chinese Academy of Sciences, PR China.
Zhang
X E
XE
Chai
Y Q
YQ
Hu
W P
WP
Zhang
Z P
ZP
Zhang
X M
XM
Cass
A E
AE
D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D017022:Flow Injection Analysis; D018189:Immunomagnetic Separation; D008163:Luminescent Measurements; D009693:Nucleic Acid Hybridization; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors
D004247:DNA