title: [The role of heat shock protein 60 (HSP60) of Helicobacter pylori in adhesion of H. pylori to human gastric epithelial cell].
abstract: Adhesion of Helicobacter pylori to both human gastric carcinoma cell lines (MKN45, MKN28 and KATO III) and prepared primary human gastric epithelial cells were analyzed with flow-cytometry. All strains adhered to human gastric carcinoma cells. Especially, these strains strongly adhered to MKN45 cells. Adhesion of H. pylori strains to prepared primary human gastric epithelial cells was also observed. However, the adherence rates of H. pylori to these cells were different among the cells used. These results suggested that the host factor might be important for adhesion of the bacteria to human gasgric cells. In addition, H20 monoclonal antibody directed to H. pylori HSP60 inhibited the adhesion of H. pylori to both cells. These results indicate that H. pylori HSP60 might be associated with the adhesion of the bacteria to human gastric epithelial cells.
authors.affiliation: Department of Microbiology, Kyorin University School of Medicine.
authors.forename: H
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Osaki
authors.forename: N
authors.initials: N
authors.lastname: Kurihara
authors.forename: H
authors.initials: H
authors.lastname: Taguchi
authors.forename: S
authors.initials: S
authors.lastname: Kamiya
mesh_terms: D001422:Bacterial Adhesion; D018834:Chaperonin 60 / Q000502:physiology*; D004847:Epithelial Cells / Q000382:microbiology; D005753:Gastric Mucosa / Q000382:microbiology*; D016480:Helicobacter pylori / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D013274:Stomach Neoplasms / Q000382:microbiology / Q000473:pathology
chemical_list: D018834:Chaperonin 60