title: Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases.
abstract: Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis.
authors.affiliation: Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Sakakibara
authors.forename: K
authors.initials: K
authors.lastname: Yanagisawa
authors.forename: Y
authors.initials: Y
authors.lastname: Takami
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Suiko
authors.forename: M C
authors.initials: MC
authors.lastname: Liu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015239:Arylsulfotransferase / Q000737:chemistry*; D019556:COS Cells; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D051379:Mice; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity; D015238:Sulfotransferases / Q000737:chemistry*; D014162:Transfection / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D015238:Sulfotransferases; D015239:Arylsulfotransferase; C028152:alcohol sulfotransferase