Production of uninfectious human immunodeficiency virus type 1 containing viral protein R fused to a single-chain antibody against viral integrase.
A single-chain antibody (scAb) against human immunodeficiency virus type 1 (HIV-1) integrase was expressed as a fusion protein of scAb and HIV-1 viral protein R (Vpr), together with the HIV-1 genome, in human 293T cells. The expression did not affect virion production much but markedly reduced the infectivity of progeny virions. The fusion protein was found to be incorporated into the virions. The incorporation appears to account for the reduced infectivity.
Okui
N
N
Division of Molecular Genetics, National Institute of Infectious Diseases, Gakuen, Musashimurayama, Tokyo, Japan.
Kobayashi
N
N
Kitamura
Y
Y
D002461:Cell Line, Transformed; D016336:Gene Products, vpr / Q000235:genetics / Q000378:metabolism*; D015483:HIV Antibodies / Q000378:metabolism*; D019427:HIV Integrase / Q000276:immunology* / Q000378:metabolism; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D014764:Viral Proteins / Q000032:analysis; D014771:Virion; D054325:vpr Gene Products, Human Immunodeficiency Virus
D016336:Gene Products, vpr; D015483:HIV Antibodies; D014764:Viral Proteins; D054325:vpr Gene Products, Human Immunodeficiency Virus; D019427:HIV Integrase