Visualization of specific B and T lymphocyte interactions in the lymph node.
Early events in the humoral immune response were visualized in lymph nodes by simultaneous tracking of antigen-specific CD4 T and B cells after immunization. The T cells were initially activated in the T cell areas when the B cells were still randomly dispersed in the B cell-rich follicles. Both populations then migrated to the edges of the follicles and interacted there, resulting in CD154-dependent B cell proliferation and germinal center formation. These results provide visual documentation of cognate T-B cell interactions and localize them to the follicular border.
Garside
P
P
University of Glasgow, Department of Immunology, Western Infirmary, Glasgow, G11 6NT, United Kingdom.
Ingulli
E
E
Merica
R R
RR
Johnson
J G
JG
Noelle
R J
RJ
Jenkins
M K
MK
D019264:Adoptive Transfer; D000818:Animals; D000917:Antibody Formation; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D023201:CD40 Ligand; D002465:Cell Movement; D003713:Dendritic Cells / Q000276:immunology; D018858:Germinal Center / Q000276:immunology; D007114:Immunization; D007075:Immunoglobulin M / Q000032:analysis; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010950:Plasma Cells / Q000276:immunology
D007075:Immunoglobulin M; D008562:Membrane Glycoproteins; D023201:CD40 Ligand