Characterization of the human analogue of a Scrapie-responsive gene.
We have recently described a novel mRNA denominated ScRG-1, the level of which is increased in the brains of Scrapie-infected mice (Dandoy-Dron, F., Guillo, F., Benboudjema, L., Deslys, J.-P., Lasmézas, C., Dormont, D., Tovey, M. G., and Dron, M. (1998) J. Biol. Chem. 273, 7691-7697). The increase in ScRG-1 mRNA in the brain follows the accumulation of PrPSc, the proteinase K-resistant form of the prion protein (PrP), and precedes the widespread neuronal death that occurs in late stage disease. In the present study, we have isolated a cDNA encoding the human counterpart of ScRG-1. Comparison of the human and mouse transcripts firmly established that both sequences encode a highly conserved protein of 98 amino acids that contains a signal peptide, suggesting that the protein may be secreted. Examination of the distribution of human ScRG-1 mRNA in adult and fetal tissues revealed that the gene was expressed primarily in the central nervous system as a 0.7-kilobase message and was under strict developmental control.
Dron
M
M
Laboratory of Viral Oncology CNRS UPR 9045, IFC1, 94801 Villejuif cedex, France. mdron@infobiogen.fr
Dandoy-Dron
F
F
Guillo
F
F
Benboudjema
L
L
Hauw
J J
JJ
Lebon
P
P
Dormont
D
D
Tovey
M G
MG
D000328:Adult; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D019286:Endopeptidase K / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics*; D018620:PrPSc Proteins / Q000235:genetics*; D017096:Prion Diseases / Q000235:genetics*; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification
D018076:DNA, Complementary; D009419:Nerve Tissue Proteins; D018620:PrPSc Proteins; D012333:RNA, Messenger; C111496:Scrg1 protein, mouse; D019286:Endopeptidase K