title: Pasteurella haemolytica ultraviolet-irradiated vaccine by parenteral and aerosol routes compared in the goat model.
abstract: Positive control 1 (PC1) (n = 9) goats were injected transthoracically into the left lung with live Pasteurella haemolytica biovar A, serovar 1 (PhA1) in polyacrylate (PA) beads on days 0 and 21. Positive control 2 (PC2) (n = 6) goats were nebulized with live PhA1 and PA beads on days 0 and 21. Negative control (NC) goats (n = 6) were each injected transthoracically into the left lung with PA beads alone on days 0 and 21. Four groups (n = 6) were administered PA beads mixed with ultraviolet (UV) killed PhA1 on days 0 and 21. The treatment doses of bacteria for these groups were principal group 1 (PR1) injected into the left lung (7.7 x 10(10) cfu); PR2, 7.7 x 10(10) UV-killed PhA1 injected subcutaneously (SC); PR3, 7.7 x 10(10) UV-killed PhA1 injected SC only on day 21; PR4, nebulized with PA beads mixed with 5.6 x 10(10) cfu of UV-killed PhA1; and PR5, nebulized with PA beads mixed with 5 x 10(8) cfu of UV-killed PhA1. All goats were challenged transthoracically in the right lung with 1 x 10(8) cfu of live PhA1 on day 42 and necropsied on day 46. The sizes of consolidated lung lesions at the challenge site were used as a measure of immunity. The data show that the introduction of live PhA1 into the lungs of goats, either by injection or aerosolization, offers excellent protection against a subsequent homologous challenge. The data also demonstrate that two transthoracic injections (21 days apart) of UV-killed PhA1 (PR1), and subcutaneous injection of UV-killed PhA1(PR2) also offer excellent protection against a subsequent homologous live PhA1 challenge. One SC injection of UV-killed PhA1 (PR3) appears to offer only partial protection against a subsequent homologous live PhA1 challenge. Inhalation of UV-killed PhA1 mixed with PA beads (PR4 and PR5) induced no protection in goats against a subsequent live PhA1 transthoracic challenge.
authors.affiliation: Conservation and Production Research Laboratory, USDA Agriculture Research Service, Bushland, TX 79012, USA.
authors.forename: C W
authors.initials: CW
authors.lastname: Purdy
authors.forename: J D
authors.initials: JD
authors.lastname: Cooley
authors.forename: D C
authors.initials: DC
authors.lastname: Straus
mesh_terms: D000336:Aerosols; D000818:Animals; D001428:Bacterial Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000627:therapeutic use; D001831:Body Temperature; D004195:Disease Models, Animal; D006041:Goats / Q000276:immunology*; D007263:Infusions, Parenteral; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D008171:Lung Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control; D008297:Male; D016978:Mannheimia haemolytica / Q000276:immunology* / Q000528:radiation effects*; D010326:Pasteurella Infections / Q000097:blood; D012007:Rectum / Q000276:immunology / Q000503:physiopathology; D014466:Ultraviolet Rays; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000336:Aerosols; D001428:Bacterial Vaccines; D014613:Vaccines, Attenuated