Epolones: novel sesquiterpene-tropolones from fungus OS-F69284 that induce erythropoietin in human cells.
In the course of our screening for small molecule modulators of erythropoietin gene expression, two novel sesquiterpene tropolones and pycnidone were isolated from a culture of OS-F69284 (ATCC 74390). Their structures were elucidated by extensive 1H and 13C NMR spectroscopic studies and chemical reactions. These compounds induced erythropoietin gene expression 5-fold at a concentration of 1-1.6 microM.
Cai
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P
MYCOsearch, a subsidiary of OSI Pharmaceuticals, 4727 University Drive, Suite 400, Durham, North Carolina 27707, USA.
Smith
D
D
Cunningham
B
B
Brown-Shimer
S
S
Katz
B
B
Pearce
C
C
Venables
D
D
Houck
D
D
D002240:Carbohydrate Sequence; D002460:Cell Line; D004921:Erythropoietin / Q000096:biosynthesis*; D005658:Fungi / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008745:Methylation; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D012717:Sesquiterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D012717:Sesquiterpenes; D004921:Erythropoietin