[Induction of apoptosis in human ovarian cancer cell line HO-8910 by transforming growth factor beta 1].
OBJECTIVE
To investigate the mechanism of inhibitory effects of Transforming Growth Factor beta 1 (TGF-beta 1) on human ovarian cancer cell in vitro.


METHODS
The possibility of induction of apoptosis in human ovarian cancer cell line HO-8910 cells after treatment with TGF-beta 1 was studied by using methods of DNA electrophoresis, P1-staining, TdT-mediated dUTP-x nick end labeling and flow cytometer assay (FCM); and the kinetic change of expression of c-myc was also studied by relative quantified RT-PCR.


RESULTS
DNA-strand nicks were present in cells after treatment with TGF-beta 1 at the final concentration of 20 ng/ml for 36 hours. The percent of labeled cells reached 75.55% on the time of 48 hours, PI staining-FCM assay also showed subdiploid peak of apoptotic cells at the same time. The typical apoptotic DNA ladder was present in genomic DNA preparation after treatment with TGF-beta 1 for 60 hours, meanwhile, the expression of c-myc in cells started to decrease beginning at treatment for 9 hours.


CONCLUSIONS
TGF-beta 1 can induce apoptosis in HO-8910; such an inductive effect may occur mainly in G0/G1 phase. The effects of TGF-beta 1 on the inhibited expression of c-myc and on the enhancement of cAMP concentration may also play important roles in the process of apoptotic induction.
Chen
X
X
Zhejiang Cancer Hospital, Hangzhou.
Mou
H
H
Dai
H
H
D017209:Apoptosis / Q000187:drug effects*; D005260:Female; D015870:Gene Expression; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D016271:Proto-Oncogene Proteins c-myc; D016212:Transforming Growth Factor beta