High sensitivity analysis of proteins and peptides by capillary electrophoresis-tandem mass spectrometry: recent developments in technology and applications.
Analytical biochemistry, in particular the analysis of regulatory proteins that control biological systems and pathways, is dependent on methods of ever-increasing sensitivity. Capillary electrophoresis (CE) has long been recognized as an ultrasensitive analytical technique. In spite of the high sensitivity, CE has not penetrated protein discovery research as a standard analytical method. In this review article we summarize recent technical developments which have significantly enhanced CE as a tool for the analysis of trace amounts of proteins. Specifically, we review recent advances in the development and application of capillary electrophoresis-mass spectrometry (CE-MS) and on-line analyte concentration techniques, and introduce the emerging field of microfluidics as a front end to mass spectrometry (MS).
Figeys
D
D
Department of Molecular Biotechnology, University of Washington, Seattle, USA. dfigeys@u.washington.edu
Aebersold
R
R
D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods* / Q000639:trends*; D010449:Peptide Mapping; D010455:Peptides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*; D012680:Sensitivity and Specificity
D010455:Peptides; D011506:Proteins