Evidence that whitefly-transmitted cowpea mild mottle virus belongs to the genus Carlavirus.
Two strains of whitefly-transmitted cowpea mild mottle virus (CPMMV) causing severe (CPMMV-S) and mild (CPMMV-M) disease symptoms in peanuts were collected from two distinct agro-ecological zones in India. The host-range of these strains was restricted to Leguminosae and Chenopodiaceae, and each could be distinguished on the basis of symptoms incited in different hosts. The 3'-terminal 2500 nucleotide sequence of the genomic RNA of both the strains was 70% identical and contains five open reading frames (ORFs). The first three (P25, P12 and P7) overlap to form a triple gene block of proteins, P32 encodes the coat protein, followed by P12 protein located at the 3' end of the genome. Genome organization and pair-wise comparisons of amino acid sequences of proteins encoded by these ORFs with corresponding proteins of known carlaviruses and potexviruses suggest that CPMMV-S and CPMMV-M are closely related to viruses in the genus Carlavirus. Based on the data, it is concluded that CPMMV is a distinct species in the genus Carlavirus.
Naidu
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RA
Crop Protection Division, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, India.
Gowda
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S
Satyanarayana
T
T
Boyko
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V
Reddy
A S
AS
Dawson
W O
WO
Reddy
D V
DV
D000595:Amino Acid Sequence; D000818:Animals; D017788:Carlavirus / Q000145:classification* / Q000235:genetics; D019663:Chenopodiaceae / Q000821:virology; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004926:Escherichia coli; D007887:Fabaceae / Q000821:virology; D016679:Genome, Viral; D007313:Insecta / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010946:Plants, Medicinal; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary