title: Induction of acrosome reaction by low temperature is comparable to physiological induction by human follicular fluid.
abstract: The acrosome reaction was induced by means of low temperature or human follicular fluid (FF) in spermatozoa from 18 patients. Live acrosome-reacted spermatozoa were determined with the triple-stain technique. Both inducers of acrosome reaction had a highly significant correlation with the percentage of acrosome-reacted spermatozoa after induction of the acrosome reaction: (r = 0.9190; P < 0.0001) and the inducibility of AR (r = 0.8472; P < 0.0001). This might indicate that both the non-physiological (low temperature) and the physiological (FF) inducers use the same signal transduction pathway, and demonstrates the usefulness of low temperature induction for diagnostic purposes.
authors.affiliation: Department of Dermatology and Andrology, Justus Liebig University, Giessen, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Henkel
authors.forename: C
authors.initials: C
authors.lastname: Müller
authors.forename: W
authors.initials: W
authors.lastname: Miska
mesh_terms: D000177:Acrosome / Q000502:physiology*; D003080:Cold Temperature; D004396:Coloring Agents; D005260:Female; D015571:Follicular Fluid / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D012044:Regression Analysis; D013084:Sperm-Ovum Interactions / Q000502:physiology*
chemical_list: D004396:Coloring Agents