Measurement of arginine carboxypeptidase-generating activity of adult plasma.
Arginine carboxypeptidase (CPR) is a novel carboxypeptidase which was first described by Campbell and Okada. CPR is generated from a stable precursor of CPR (proCPR) during coagulation or under other circumstances and is promptly inactivated at 37 C. Therefore, it is not easy to determine CPR in blood samples. Since proCPR can be separated from the other basic carboxypeptidase (carboxypeptidase N; CPN) by passing plasma through DEAE gel, we have established a method to determine the amount of proCPR after converting it to active CPR by trypsin treatment. We first separated the proCPR from CPN using a filter cup tube (FC tube) packed with DEAE Sephadex, and measured activity after conversion of the enzyme to its active form using trypsin. With this method, no significant decrease in proCPR was noted in the plasma of patients including those with rheumatoid arthritis (RA), although CPR activity in fresh sera has been reported to be decreased. This discrepancy suggests that proCPR is not depleted in most patient sera, but that the level of activity of the enzyme which converts proCPR into active CPR may be compromised in RA patients.
Watanabe
M
M
Department of Molecular Biology, Nagoya City University School of Medicine, Nagoya, Aichi, Japan.
Ishikawa
Y
Y
Campbell
W
W
Okada
H
H
D000328:Adult; D017677:Age Distribution; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000201:enzymology; D002850:Chromatography, Gel / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D001121:Lysine Carboxypeptidase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results
D001121:Lysine Carboxypeptidase