Transforming growth factor-beta1, insulin-like growth factors, and insulin-like growth factor binding proteins in ovarian follicular fluid are differentially regulated by the type of ovarian hyperstimulation used for in vitro fertilization.
OBJECTIVE
To determine the effects of hMG and highly purified FSH on follicular production of the ovarian growth factors transforming growth factor-beta1 (TGF-beta1), insulin-like growth factors I and II (IGF-I and IGF-II), and insulin-like growth factor binding proteins-1 and -3 (IGFBP-1 and IGFBP-3).


METHODS
Controlled clinical study.


METHODS
University IVF program.


METHODS
One hundred twenty women who were <38 years old and had a >3-year duration of infertility in their present relationship participated in the study.


METHODS
Follicular fluid and matched serum were collected at oocyte pick-up and analyzed for growth factors and E2 with the use of ELISA and RIA.


METHODS
Levels of TGF-beta1, IGF-I. IGF-II, IGFBP-1 and IGFBP-3 in follicular fluid and levels of E2 in serum were measured.


RESULTS
Compared with highly purified FSH, ovarian hyperstimulation with hMG produced lower levels of TGF-beta1 and IGF-I and higher levels of IGFBP-1. Levels of IGF-II and IGFBP-3 were similar with the 2 treatments.


CONCLUSIONS
In patients undergoing IVF, the follicular expression of TGF-beta1, IGF-I, and IGFBP-1 was regulated differently by highly purified FSH compared with a preparation containing FSH and LH in a 1:1 ratio (hMG). The results indicate that FSH and LH control ovarian production of these growth factors differentially.
Fried
G
G
Department of Woman and Child Health, Karolinska Hospital, Stockholm, Sweden. GABRIEL.FRIED@FYFA.KI.SE
Wramsby
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H
Tally
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D000328:Adult; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018969:Insulin-Like Growth Factor Binding Proteins / Q000378:metabolism*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology; D010053:Ovary / Q000502:physiology*; D010062:Ovulation Induction; D011863:Radioimmunoassay; D013002:Somatomedins / Q000378:metabolism*; D013268:Stimulation, Chemical; D013799:Theca Cells / Q000378:metabolism / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000378:metabolism*
D018969:Insulin-Like Growth Factor Binding Proteins; D013002:Somatomedins; D016212:Transforming Growth Factor beta; D004958:Estradiol