An isoelectrically trapped enzyme reactor operating in an electric field.
Membrane enzyme reactors constitute an attempt at integrating catalytic conversion, product separation and/or concentration and catalyst recovery into a single operation. Whereas conventional membrane reactors confine an enzyme, in a free form, to one side of a membrane by size exclusion, electrostatic repulsion, or physical or chemical immobilization onto an intermediate support (gel, liposome), the membrane reactor here described is shown to operate under an entirely new principle: enzyme confinement into an isoelectric trap located in a multicompartment electrolyzer operating in an electric field. Two isoelectric membranes, having pI values encompassing both the enzyme pI and the pH of its optimum of activity, act by continuously titrating the enzyme trapped inside, thus preventing it from escaping the reaction chamber. Charged products generated by the enzyme catalysis are continuously electrophoretically transported away from the reaction chamber and collected into other chambers stacked either towards the cathodic or anodic sides. In a urease reactor, ammonia is continuously harvested towards the cathode, thus allowing >95% substrate consumption with maintenance of enzyme integrity over much longer time periods than in a batch reactor. In a trypsin reactor, casein is digested and biologically active peptides are continuously harvested in a pure form into appropriate isoelectric traps. In a third example, pure D-phenylglycine is produced from a racemate mixture, via an acylation reaction onto a cosubstrate (the ester methyl-4-hydroxyphenyl acetate), brought about by the enzyme penicillin G acylase.
Righetti
P G
PG
University of Verona, Department of Agricultural and Industrial Biotechnologies, Italy. righetti@imiucca.csi.unimi.it
Bossi
A
A
D002364:Caseins / Q000737:chemistry; D004560:Electricity; D004800:Enzymes, Immobilized / Q000737:chemistry*; D007526:Isoelectric Point; D010399:Penicillin Amidase / Q000737:chemistry; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry; D014510:Urease / Q000737:chemistry
D002364:Caseins; D004800:Enzymes, Immobilized; D014357:Trypsin; D010399:Penicillin Amidase; D014510:Urease