A species-specific satellite DNA from the entomopathogenic nematode Heterorhabditis indicus.
An AluI satellite DNA family has been cloned from the entomopathogenic nematode Heterorhabditis indicus. This repeated sequence appears to be an unusually abundant satellite DNA, since it constitutes about 45% of the H. indicus genome. The consensus sequence is 174 nucleotides long and has an A + T content of 56%, with the presence of direct and inverted repeat clusters. DNA sequence data reveal that monomers are quite homogeneous. Such homogeneity suggests that some mechanism is acting to maintain the homogeneity of this satellite DNA, despite its abundance, or that this repeated sequence could have appeared recently in the genome of H. indicus. Hybridization analysis of genomic DNAs from different Heterorhabditis species shows that this satellite DNA sequence is specific to the H. indicus genome. Considering the species specificity and the high copy number of this AluI satellite DNA sequence, it could provide a rapid and powerful tool for identifying H. indicus strains.
Abadon
M
M
Laboratoire de biologie des invertébrés, Insitut national de la recherche agronomique (INRA), Antibes, France. abad@antibes.inra.fr
Grenier
E
E
Laumond
C
C
Abad
P
P
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018509:DNA, Helminth / Q000737:chemistry*; D004276:DNA, Satellite / Q000737:chemistry*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D017168:Rhabditida / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D018509:DNA, Helminth; D004276:DNA, Satellite; C024669:endodeoxyribonuclease AluI; D015252:Deoxyribonucleases, Type II Site-Specific