Increased concentrations of calcitonin gene-related peptide-like immunoreactivity in rat brain and peripheral tissue after ischaemia: correlation to flap survival.
The effects of experimentally induced ischaemia after free-flap surgery on concentrations of neuropeptide Y (NPY), neurokinin A (NKA), substance P (SP) and calcitonin gene-related peptide (CGRP)-like immunoreactivity (-LI) were studied in flap tissue and in different regions of the rat brain (striatum, hippocampus, pituitary, hypothalamus, frontal and occipital cortex). Ten days after the operation, CGRP-LI and NKA-LI were decreased in the ischaemic tissue but increased in the surrounding tissue. In the brain, CGRP-LI was increased in five of six regions analysed, with the exception of the striatum. SP-LI and NKA-LI were increased in the pituitary and hippocampus, but decreased in other brain regions. Changes of CGRP-LI in the brain correlated positively with the CGRP-LI concentrations in the surrounding flap tissue and the CGRP-LI concentrations in the ischaemic flap tissue with the extent of flap survival. The results of the present study suggest that higher concentrations of CGRP-LI are related to tissue survival and that endogenous CGRP has a regulatory effect in ischaemia.
Bucinskaite
V
V
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden. violeta.bucinskaite@fyfa.ki.se
Brodda-Jansen
G
G
Stenfors
C
C
Theodorsson
E
E
Lundeberg
T
T
D000818:Animals; D001706:Biopsy; D001923:Brain Chemistry / Q000502:physiology*; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D002540:Cerebral Cortex / Q000737:chemistry / Q000378:metabolism; D003429:Cross Reactions; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D015288:Neurokinin A / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D009478:Neuropeptide Y / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011921:Rats, Inbred WKY; D013373:Substance P / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D013524:Surgical Flaps / Q000098:blood supply* / Q000294:innervation* / Q000473:pathology; D013564:Sympathetic Nervous System / Q000737:chemistry
D009478:Neuropeptide Y; D013373:Substance P; D015288:Neurokinin A; D015740:Calcitonin Gene-Related Peptide