Detection of negative allosteric effects between monoclonal antibodies by using an antigenic model-builder computer program.
The ability of monoclonal antibodies (MAb) to bind or not simultaneously to the antigen (Ag) is used to establish antigenic maps considering that two different MAb do not bind to the Ag when the corresponding epitopes are overlapped (steric effect). Nevertheless, MAb inducing negative allosteric effect on the Ag could prevent the binding of the second MAb even if it is directed to a separate epitope. We report here that a knowledge-based expert module included in our previously described antigenic model-builder program (MAPAG) was able to differentiate between steric and negative allosteric effects between some MAb.
Aguilar
R C
RC
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.
Blank
V C
VC
Retegui
L A
LA
Roguin
L P
LP
D000465:Algorithms; D000495:Allosteric Site / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000936:Antigen-Antibody Complex / Q000276:immunology; D001185:Artificial Intelligence; D001666:Binding Sites, Antibody / Q000276:immunology*; D003198:Computer Simulation; D000939:Epitopes / Q000276:immunology*; D005103:Expert Systems; D006801:Humans; D007091:Image Processing, Computer-Assisted; D018448:Models, Immunological; D012984:Software
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000939:Epitopes