Intestinal absorption of bile acids: paradoxical behaviour of the 14 kDa ileal lipid-binding protein in differential photoaffinity labelling.
Photoaffinity labelling of brush border membrane vesicles from rabbit ileum with radiolabelled 3,3-azo and 7,7-azo derivatives of taurocholate identified integral membrane proteins of molecular masses 93 and 46 kDa, as well as a 14 kDa peripheral membrane protein, as components of the ileal Na+/bile acid transport system [Kramer, Girbig, Gutjahr, Kowalewski, Jouvenal, Müller, Tripier and Wess (1993) J. Biol. Chem. 268, 18035-18046]. Differential photoaffinity labelling in the presence of non-radiolabelled bile acid derivatives led, as expected, to a concentration-dependent decrease in the extent of labelling of the 93 and 46 kDa transmembrane proteins, which are the monomeric and dimeric forms of the ileal bile acid transporter protein. The extent of labelling of the 14 kDa ileal lipid-binding protein (ILBP), however, increased on the addition of unlabelled bile acids, the increase being dependent on the structure of the bile acid added. The possibility of artifacts was excluded by photoaffinity labelling experiments in the frozen state as well as by model calculations. The experimental results suggest that the binding of bile acids to ILBP can increase the affinity of ILBP for bile acids. These results would be in accordance with a substrate-load modification of transport activity and a positive-feedback regulation mechanism for active uptake of bile acid in the ileum.
Kramer
W
W
Research Metabolic Diseases, Hoechst Marion Roussel Deutschland GmbH, D-65926 Frankfurt am Main, Federal Republic of Germany.
Corsiero
D
D
Friedrich
M
M
Girbig
F
F
Stengelin
S
S
Weyland
C
C
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004347:Drug Interactions; D007408:Intestinal Absorption; D008871:Microvilli / Q000378:metabolism; D008956:Models, Chemical; D008970:Molecular Weight; D029363:Organic Anion Transporters, Sodium-Dependent; D019920:Photoaffinity Labels; D010777:Photochemistry; D011817:Rabbits; D027981:Symporters
D001647:Bile Acids and Salts; D002352:Carrier Proteins; D029363:Organic Anion Transporters, Sodium-Dependent; D019920:Photoaffinity Labels; D027981:Symporters; C071908:sodium-bile acid cotransporter