title: Virulence factors of Staphylococcus aureus in the pathogenesis of endocarditis. A comparative study of clinical isolates.
abstract: It is now generally accepted that adherence of microorganisms to various components of cardiac valve surfaces or vegetation lodging on the heart valves is an important early event in the pathogenesis of infective endocarditis. 120 clinical isolates of S. aureus obtained from patients with endocarditis and wound infections and from nasopharyngeal carriers were quantitatively analysed in vitro for their ability to bind to fibronectin and to produce protein A and alpha-toxin. Both cell-bound and extracellular protein A were measured and alpha-toxin was determined as antigen and as haemolytic activity. The highest fibronectin binding ability was found in carrier strains while no significant differences between strains were observed regarding the production of protein A. Strains isolated from patients with endocarditis produced significantly lower amounts of alpha-toxin than did strains from the other two groups. An inverse relationship between the production of protein A and of alpha-toxin was noticed in the material. Animal passage of five strains in an experimental endocarditis model showed a good reproducibility of the test systems and one strain was upregulated in its fibronectin binding ability and in alpha-toxin production. These in vitro results indicate that the fibronectin binding ability is not the decisive adherence factor and question the role of alpha-toxin as a virulence factor in endocarditis.
authors.affiliation: Microbiology and Tumorbiology Center (MTC), Karolinska Institute, Stockholm, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Nozohoor
authors.forename: A
authors.initials: A
authors.lastname: Heimdahl
authors.forename: P
authors.initials: P
authors.lastname: Colque-Navarro
authors.forename: I
authors.initials: I
authors.lastname: Julander
authors.forename: B
authors.initials: B
authors.lastname: Söderquist
authors.forename: R
authors.initials: R
authors.lastname: Möllby
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000378:metabolism*; D004195:Disease Models, Animal; D004697:Endocarditis, Bacterial / Q000382:microbiology*; D005353:Fibronectins / Q000378:metabolism; D006460:Hemolysin Proteins / Q000378:metabolism*; D006801:Humans; D051381:Rats; D013205:Staphylococcal Protein A / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000378:metabolism* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D001427:Bacterial Toxins; D005353:Fibronectins; D006460:Hemolysin Proteins; D013205:Staphylococcal Protein A; C021091:staphylococcal alpha-toxin