Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE.
The genomic DNA of the BE strain of Escherichia coli has been scrutinized to detect porin genes that have not been identified so far. Southern blot analysis yielded two DNA segments which proved highly homologous to, yet distinct from, the ompC, ompF, and phoE porin genes. The two genes were cloned and sequenced. One of them, designated ompN, encodes a porin which, due to low levels of expression, has eluded prior identification. The functional properties (single-channel conductance) of the OmpN porin, purified to homogeneity, closely resemble those of the OmpC porin from E. coli K-12. The second DNA fragment detected corresponds to the nmpC gene, which, due to an insertion of an IS1 element in its coding region, is not expressed in E. coli BE.
Prilipov
A
A
Department of Microbiology, Biozentrum, University of Basel, Switzerland.
Phale
P S
PS
Koebnik
R
R
Widmer
C
C
Rosenbusch
J P
JP
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D050260:Carbohydrate Metabolism; D003001:Cloning, Molecular; D000939:Epitopes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005838:Genotype; D008051:Lipid Bilayers; D008969:Molecular Sequence Data; D010957:Plasmids; D018272:Porins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000939:Epitopes; D029968:Escherichia coli Proteins; D008051:Lipid Bilayers; C088795:NmpC protein, E coli; D018272:Porins; D011994:Recombinant Proteins; C113199:ompN protein, E coli