A second-step splicing activity is conserved from yeast to human.
We describe a defective HeLa nuclear extract which is particularly deficient in step 2 of splicing reaction. With this extract we have studied the conservation of a second-step activity from yeast to human cells. We detected a S. cerevisiae second-step splicing activity that allows restoration of step 2 of the defective HeLa nuclear extract, which indicates that yeast purified fraction has a second-step activity that is conserved from yeast to human cells. The activity is a yeast UsnRNP protein(s) since it is purified with anti-trimethylguanosine by immunoaffinity columns.
Cicarelli
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RM
CID-CSIC, c/Jorge Girona Salgado 18-26, Barcelona, 08034, Spain.
Khaouja
A
A
Codony
C
C
Espuny
R
R
Eritja
R
R
Bach-Elias
M
M
D005075:Biological Evolution; D002846:Chromatography, Affinity; D005816:Genetic Complementation Test; D005914:Globins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics; D017411:Ribonucleoproteins, Small Nuclear / Q000302:isolation & purification / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism
D012322:RNA Precursors; D017411:Ribonucleoproteins, Small Nuclear; D005914:Globins