Multiple phosphotyrosine phosphatase mRNAs are expressed in the human lung fibroblast cell line WI-38.
Protein tyrosine phosphatases are important components of signal transduction pathways. The authors have used reverse transcription/polymerase chain reactions to accomplish a comprehensive examination of the RNA expression for 58 distinct mammalian protein tyrosine and dual specificity phosphatase (PTPase) and PTPase-like genes in the normal human diploid fibroblast cell line WI-38. Thirty-seven of these PTPase genes express easily measurable RNA, and four simultaneously express the RNA for two or more isoforms. Messages for an additional eight PTPase genes are detectable at low levels. Only 14 known PTPase genes do not express measurable RNA under our conditions. For purposes of comparison, the authors also assessed the PTPases expressed in the WI-38 cell line using highly degenerate primers to conserved motifs found in the classical tyrosine-specific PTPases. Only eight of the 22 classic tyrosine-specific PTPases detected using the specific primers were detected using these degenerate primers. Our panel of specific PTPase primers should be very useful for semiquantitatively assessing the repertoire of PTPases expressed by cells.
Dayton
M A
MA
Center for Excellence in Cancer Research, Treatment and Education, and Department of Medicine, Louisiana State University Medical Center, Shreveport 71130-3932, USA.
Knobloch
T J
TJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence; D017931:DNA Primers; D005347:Fibroblasts; D006801:Humans; D008168:Lung; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000502:physiology*; D009626:Terminology as Topic; D014158:Transcription, Genetic
D017931:DNA Primers; D012333:RNA, Messenger; D017027:Protein Tyrosine Phosphatases