title: Inhibition of ascorbate peroxidase under oxidative stress in tobacco having bacterial catalase in chloroplasts.
abstract: To analyze the potential of the active oxygen-scavenging system of chloroplasts, we introduced Escherichia coli catalase into tobacco chloroplasts. Photosynthesis of transgenic plants was tolerant to high irradiance under drought conditions, while the wild plants suffered severe damage in photosynthesis under the same conditions. Irrespective of responses to the stress, ascorbate peroxidase was completely inactivated both in the transgenic and wild-type plants. These findings are contrary to the established idea that the ascorbate peroxidase-mediated antioxidative system protects chloroplasts from oxidative stress.
authors.affiliation: Research Institute of Innovative Technology for the Earth, Kizu, Kyoto, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shikanai
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Yamauchi
authors.forename: S
authors.initials: S
authors.lastname: Sano
authors.forename: K I
authors.initials: KI
authors.lastname: Tomizawa
authors.forename: A
authors.initials: A
authors.lastname: Yokota
authors.forename: S
authors.initials: S
authors.lastname: Shigeoka
mesh_terms: D060387:Ascorbate Peroxidases; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002736:Chloroplasts / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D018384:Oxidative Stress; D010544:Peroxidases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010947:Plants, Toxic; D014026:Nicotiana / Q000201:enzymology / Q000378:metabolism*
chemical_list: D010544:Peroxidases; C102664:hydroperoxidase II; D060387:Ascorbate Peroxidases; D002374:Catalase