Phosphorylation of stathmin modulates its function as a microtubule depolymerizing factor.
Oncoprotein 18 or stathmin was isolated from bovine brain, characterized and novel features of its function as a microtubule depolymerizing factor were tested. The effect of phosphorylation of stathmin on its function as a microtubule depolymerizing factor has been tested in vitro. Five different protein kinases, protein kinase A, MAP kinase, cdc2 kinase, glycogen synthase kinase 3 and casein kinase 2, were used to modify stathmin, since it is known that these kinases could phosphorylate several residues that are modified in vivo and could have important roles in stathmin function. The residues phosphorylated in vitro by the different protein kinases were identified and in some cases they correspond to those modified in vivo. Recombinant unphosphorylated stathmin and native stathmin, which was previously dephosphorylated with alkaline phosphatase, showed similar microtubule depolymerizing activity. This activity is higher than that of stathmin phosphorylated by protein kinase A, MAP kinase or cdc 2 kinase, whereas phosphorylation of the protein with casein kinase 2 or glycogen synthase kinase 3 resulted in a slight increase of the depolymerizing activity.
Moreno
F J
FJ
Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Cantoblanco, Madrid, Spain.
Avila
J
J
D000818:Animals; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism / Q000502:physiology; D001923:Brain Chemistry; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism; D002417:Cattle; D038362:Glycogen Synthase Kinase 3; D038341:Glycogen Synthase Kinases; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology*; D010766:Phosphorylation; D050777:Stathmin
D001704:Biopolymers; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D010750:Phosphoproteins; D050777:Stathmin; D038341:Glycogen Synthase Kinases; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D038362:Glycogen Synthase Kinase 3