Ly-49D and Ly-49H associate with mouse DAP12 and form activating receptors.
Several members of the Ly-49 receptor family inhibit NK cell-mediated lysis of targets expressing appropriate MHC class I molecules. Ly-49D and Ly-49H, two Ly-49 molecules that lack immunoreceptor tyrosine-based inhibitory motifs (ITIM) in their cytoplasmic domains, associate with mouse DAP12, a molecule that possesses an immunoreceptor tyrosine-based activation motif (ITAM). Cotransfection of either Ly-49D or Ly-49H with DAP12 induces surface expression of both Ly-49 and DAP12. The Ly-49/DAP12 complex was coimmunoprecipitated from the transfected cells, demonstrating a physical association of DAP12 with Ly-49D or Ly-49H in the plasma membrane. Stimulation of transfectants with Abs recognizing either Ly-49D or Ly-49H results in cellular activation, as assessed by induction of tyrosine phosphorylation of multiple cellular substrates.
Smith
K M
KM
DNAX Research Institute, Palo Alto, CA 94304, USA. ksmith@dnax.org
Wu
J
J
Bakker
A B
AB
Phillips
J H
JH
Lanier
L L
LL
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D000950:Antigens, Ly; D000954:Antigens, Surface / Q000378:metabolism*; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000276:immunology / Q000378:metabolism*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins; D051379:Mice; D017459:Receptors, Amino Acid / Q000276:immunology; D011971:Receptors, Immunologic / Q000378:metabolism* / Q000502:physiology*; D055630:Receptors, NK Cell Lectin-Like; D014158:Transcription, Genetic / Q000276:immunology; D014162:Transfection / Q000276:immunology; D014443:Tyrosine / Q000378:metabolism
D048868:Adaptor Proteins, Signal Transducing; D000950:Antigens, Ly; D000954:Antigens, Surface; D007376:Interleukin-2; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D017459:Receptors, Amino Acid; D011971:Receptors, Immunologic; D055630:Receptors, NK Cell Lectin-Like; C110546:TYROBP protein, human; C087659:tyrosine receptor; D014443:Tyrosine