title: RT-PCR analysis of mRNA expression of natriuretic peptide family and their receptors in rat inner ear.
abstract: To assess the possible physiological role of the atrial natriuretic peptide (ANP) family, we investigated the expression of mRNA of ANP, brain natriuretic peptide (BNP), C-type natriuretic peptide (CNP), and their receptors in rat inner ear using the reverse transcription-polymerase chain reaction method. ANP and CNP message bands were detected in the inner ear, but the BNP message band was not. Amplification products of the expected sizes of ANP-A, ANP-B and ANP-C receptors were detected in the inner ear. These results suggest that natriuretic peptide family may influence the function of the inner ear through the ANP-A, ANP-B, and ANP-C receptors.
authors.affiliation: Department of Otolaryngology, Shiga University of Medical Science, Otsu, Japan. miki@belle.shiga-med.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Kitano
authors.forename: T
authors.initials: T
authors.lastname: Kitanishi
authors.forename: Y
authors.initials: Y
authors.lastname: Yazawa
authors.forename: K
authors.initials: K
authors.lastname: Kitajima
authors.forename: T
authors.initials: T
authors.lastname: Takeda
authors.forename: H
authors.initials: H
authors.lastname: Kimura
authors.forename: I
authors.initials: I
authors.lastname: Tooyama
mesh_terms: D000818:Animals; D009320:Atrial Natriuretic Factor / Q000096:biosynthesis / Q000235:genetics*; D007758:Ear, Inner / Q000378:metabolism*; D006162:Guanylate Cyclase / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D005810:Multigene Family; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D017461:Receptors, Atrial Natriuretic Factor / Q000096:biosynthesis / Q000145:classification / Q000235:genetics*; D000159:Vestibulocochlear Nerve / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D011506:Proteins; D012333:RNA, Messenger; C058930:brain natriuretic peptide receptor; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D006162:Guanylate Cyclase; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C