title: Seasonal changes in the Plasmodium falciparum population in individuals and their relationship to clinical malaria: a longitudinal study in a Sudanese village.
abstract: Residents of Daraweesh village in Sudan were monitored for Plasmodium falciparum infection and malaria morbidity in 3 malaria seasons from 1993 to 1996. Malaria parasites were detected microscopically and by polymerase chain reaction (PCR) in a series of cross-sectional surveys. PCR revealed submicroscopical infections during the dry season, particularly among individuals who had recovered from a malaria episode following successful drug treatment. Clinical and subclinical infections were contrasted by assaying for allelic polymorphism at 2 gene loci, MSP-1 and GLURP and 2 hypotheses examined with reference to these data: that clinical malaria is associated with infection with novel parasite genotypes not previously detected in that host, or alternatively, that clinical malaria episodes are associated with an increased number of clones in an infection. We detected more mixed infections among clinical isolates, but people carrying parasites during the dry season were not found to have an increased risk of disease in the following malaria season. There was a clear association of disease with the appearance of novel parasite genotypes.
authors.affiliation: Institute of Cell, Animal and Population Biology, University of Edinburgh, UK. c.roper@lshtm.ac.uk
authors.forename: C
authors.initials: C
authors.lastname: Roper
authors.forename: W
authors.initials: W
authors.lastname: Richardson
authors.forename: I M
authors.initials: IM
authors.lastname: Elhassan
authors.forename: H
authors.initials: H
authors.lastname: Giha
authors.forename: L
authors.initials: L
authors.lastname: Hviid
authors.forename: G M
authors.initials: GM
authors.lastname: Satti
authors.forename: T G
authors.initials: TG
authors.lastname: Theander
authors.forename: D E
authors.initials: DE
authors.lastname: Arnot
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D015331:Cohort Studies; D005838:Genotype; D006801:Humans; D008137:Longitudinal Studies; D016778:Malaria, Falciparum / Q000097:blood / Q000453:epidemiology*; D009017:Morbidity; D010963:Plasmodium falciparum / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012621:Seasons; D013397:Sudan / Q000453:epidemiology