title: Increased excretion of aromatic amino acid catabolites in animals infected with Trypanosoma brucei evansi.
abstract: Aromatic amino acid catabolism by Trypanosoma brucei evansi was investigated in vivo using C3HeB/FeJ mice. The major catabolites detected by gas chromatography in the urines of infected animals were phenylpyruvic acid, 4-hydroxyphenylpyruvic acid, and indole-3-pyruvic acid. Identity of each compound was confirmed by gas chromatography-mass spectrometry. Concentrations of catabolites in urine of infected mice were correlated with parasitemia and returned to normal following suramin treatment. Other aromatic amino acid metabolites, including indole-3-acetic acid, indole-3-lactic acid, and 4-hydroxyphenyllactic acid, were detected in urine from infected animals by gas chromatography mass spectrometry, although quantities were too low to be quantified reproducibly. Both phenylpyruvic acid and 4-hydroxyphenylpyruvic acid were also detected in urine of dogs and donkeys experimentally infected in Egypt with a recent field isolate of T. b. evansi. Tryptophan metabolites could not be assayed in dog and urine samples because formalin, which degraded the indole acids, had to be added before the samples could be imported into the U.S. Finally, concentrations of urinary catabolites during infection were correlated with the tyrosine aminotransferase activity in infected mouse sera.
authors.affiliation: Department of Animal Science, Faculty of Veterinary Medicine, Moshtohour Tukh, Benha, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: El Sawalhy
authors.forename: J R
authors.initials: JR
authors.lastname: Seed
authors.forename: J E
authors.initials: JE
authors.lastname: Hall
authors.forename: H
authors.initials: H
authors.lastname: El Attar
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D004283:Dog Diseases / Q000652:urine; D004285:Dogs; D018397:Equidae / Q000469:parasitology / Q000652:urine; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000662:veterinary; D006841:Hydrocarbons, Aromatic / Q000378:metabolism; D007211:Indoles / Q000652:urine*; D008099:Liver / Q000201:enzymology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010667:Phenylpyruvic Acids / Q000652:urine*; D013498:Suramin / Q000627:therapeutic use; D014344:Trypanocidal Agents / Q000627:therapeutic use; D014353:Trypanosomiasis, African / Q000188:drug therapy / Q000652:urine / Q000662:veterinary*; D014444:Tyrosine Transaminase / Q000097:blood / Q000378:metabolism
chemical_list: D000596:Amino Acids; D006841:Hydrocarbons, Aromatic; D007211:Indoles; D010667:Phenylpyruvic Acids; D014344:Trypanocidal Agents; C010590:4-hydroxyphenylpyruvic acid; C008122:indol-3-yl pyruvic acid; D013498:Suramin; D014444:Tyrosine Transaminase; C031606:phenylpyruvic acid