[Cytotoxicity of chemicals used in household products: estimation of eye irritating potency of 25 chemicals tested during 1991-1996].
Cytotoxicity potential of chemicals was evaluated by determining the concentrations inducing 50% reduction of neutral red (NR) uptake into Chinese hamster fibroblast V79 cells compared with control culture (IC50). The results of cytotoxicity test for surfactants with the data produced by the in vivo Draize eye and skin irritation test were compared. There was a good correlation between cytotoxicity and eye irritation score obtained from the Draize test. In contrast, no correlation was observed between Draize skin irritation score and cytotoxic potential of chemicals. Therefore, the NR cytotoxicity test was regarded as a possible in vitro model for predicting eye irritation. Based on the IC50 values in the NR cytotoxicity test, the eye irritation classification (weak, moderate and strong) for each chemical used in household products has been established. We evaluated the cytotoxicity of 25 chemicals used for antimicrobial, rubber accelerator, rubber antioxidant, ultraviolet absorber etc. in household products, and estimated the eye irritating potency of these test chemicals according to the criterion.
Ikarashi
Y
Y
ikarashi@nihs.go.jp
Tsuchiya
T
T
Nakamura
A
A
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005123:Eye / Q000187:drug effects*; D005347:Fibroblasts; D006795:Household Products / Q000633:toxicity*; D066298:In Vitro Techniques; D007509:Irritants; D009499:Neutral Red; D012867:Skin / Q000187:drug effects
D007509:Irritants; D009499:Neutral Red