p53 protein is a suppressor of papillomavirus DNA amplificational replication.
p53 protein was able to block human and bovine papillomavirus DNA amplificational replication while not interfering with Epstein-Barr virus oriP once-per-cell cycle replication. Oligomerization, intact DNA-binding, replication protein A-binding, and proline-rich domains of the p53 protein were essential for efficient inhibition, while the N-terminal transcriptional activation and C-terminal regulatory domains were dispensable for the suppressor activity of the p53 protein. The inhibition of replication was caused neither by the downregulation of expression of the E1 and E2 proteins nor by cell cycle block or apoptosis. Our data suggest that the intrinsic activity of p53 to suppress amplificational replication of the papillomavirus origin may have an important role in the virus life cycle and in virus-cell interactions.
Lepik
D
D
Department of Microbiology and Virology, Institute of Molecular and Cell Biology, Tartu University and Estonian Biocentre, Tartu EE2400, Estonia.
Ilves
I
I
Kristjuhan
A
A
Maimets
T
T
Ustav
M
M
D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D052688:Bovine papillomavirus 1 / Q000235:genetics* / Q000502:physiology; D016466:CHO Cells; D019556:COS Cells; D002417:Cattle; D002453:Cell Cycle; D006224:Cricetinae; D004261:DNA Replication; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000235:genetics; D015536:Down-Regulation; D005784:Gene Amplification; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics* / Q000502:physiology; D018741:Replication Origin; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C069814:E1 protein, Bovine papillomavirus; C051550:E2 protein, Bovine papillomavirus; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53; D014764:Viral Proteins