Potentiation by isoproterenol on carbachol-induced K+ and Cl- currents and fluid secretion in rat parotid.
Isoproterenol (IPR) and 8-(4-chlorophenylthio)-cyclic AMP (cpt-cAMP) enhanced carbachol (CCh)-induced fluid secretion from rat parotid glands, but had no effect by themselves. The enhancement by IPR was blocked by propranolol. In dispersed parotid acinar cells, IPR and cpt-cAMP potentiated CCh-induced K+ and Cl- currents (IK and ICl). IPR at the concentration of 0.1 microM significantly potentiated the CCh-induced increase in intracellular Ca2+ concentration ([Ca2+]i), but 1 mm cpt-cAMP did not. The incidence of the potentiation by IPR in CCh-induced Mn2+ entry was 31% and that by cpt-cAMP was 21%. The potentiation by IPR in the ionic currents and the [Ca2+]i was suppressed by propranolol. These results suggest that the CCh-induced fluid secretion from rat parotid glands is enhanced by IPR through the potentiation of IK and ICl mainly by the increased cyclic AMP level and partially by the potentiated Ca2+ influx and [Ca2+]i increase, and that IPR is more effective than cpt-cAMP in the enhancement of the CCh-induced [Ca2+]i increase.
Hirono
C
C
Department of Oral Physiology, Hiroshima University School of Dentistry, 2-3 Kasumi 1 Chome, Minami-ku, Hiroshima 734, Japan.
Sugita
M
M
Furuya
K
K
Yamagishi
S
S
Shiba
Y
Y
D000818:Animals; D001826:Body Fluids / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002217:Carbachol / Q000494:pharmacology*; D018118:Chloride Channels / Q000378:metabolism*; D000242:Cyclic AMP / Q000502:physiology; D004357:Drug Synergism; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D008345:Manganese / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D010306:Parotid Gland / Q000187:drug effects* / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012463:Saliva / Q000187:drug effects / Q000378:metabolism
D018118:Chloride Channels; D015221:Potassium Channels; D008345:Manganese; D002217:Carbachol; D011433:Propranolol; D000242:Cyclic AMP; D007545:Isoproterenol; D002118:Calcium