Expression of the human immunodeficiency virus gag gene products by a replication-incompetent herpes simplex virus vector.
A recombinant replication-incompetent herpes simplex virus vector (vd120/Gag) that expressed the human immunodeficiency virus 1 gag gene and part of the pol gene that encodes the HIV-1 protease was constructed. Examination of cells infected with vd120/Gag revealed the presence of the Gag polyprotein Pr55gag by 12 h post-infection, as well as abundant levels of the proteolytically processed 24-kDa capsid protein. Analysis of vector-infected cells and culture supernatant indicated that the majority of the 24-kDa protein remained cell-associated. Although the HIV-1 Gag polyprotein was produced in vd120/Gag-infected cells, there was no evidence of HIV virus-like particle production upon examination of vector-infected cells by transmission electron microscopy.
Kessler
J
J
Laboratory of DNA Viruses, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Muller
J
J
Weir
J P
JP
D000818:Animals; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D015870:Gene Expression; D015750:Genes, gag; D005822:Genetic Vectors; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000235:genetics*; D018259:Herpesvirus 1, Human / Q000502:physiology; D006801:Humans; D008854:Microscopy, Electron; D011499:Protein Processing, Post-Translational; D013997:Time Factors; D014709:Vero Cells; D014779:Virus Replication
D016655:HIV Core Protein p24