Continuous ethanol production from sugarcane molasses using a column reactor of immobilized Saccharomyces cerevisiae HAU-1.
A fermentation system for the continuous ethanol production from sucrose and molasses using calcium alginate immobilized Saccharomyces cerevisiae strain HAU-1 has been optimised. Immobilization of active yeast cells (30%, w/v) was accomplished in 1.5% calcium alginate and these yeast-beads were employed for ethanol production in a vertical column reactor. High ethanol productivity was achieved with a medium containing 10%, w/v sucrose at a dilution rate of 0.20 h-1. Alginate beads containing 30-50% yeast cell--mass resulted in high productivity 60.4 g l-1 h-1). Sugarcane molasses containing 15% total sugars was fermented in column reactor to produce 6.0%, v/v ethanol in continuous system. These yields could be improved to 7.0% with overall 11% increase in fermentation--efficiency with the use of acid-clarified sugar cane molasses medium.
Sheoran
A
A
Department of Microbiology, CCS Haryana Agricultural University, Hisar, India.
Yadav
B S
BS
Nigam
P
P
Singh
D
D
D000464:Alginates; D018533:Biomass; D019149:Bioreactors; D018914:Cells, Immobilized; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D007218:Industrial Microbiology / Q000379:methods*; D008863:Microspheres; D008965:Molasses; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013395:Sucrose / Q000378:metabolism*
D000464:Alginates; D006603:Hexuronic Acids; D000431:Ethanol; D013395:Sucrose; D020723:Glucuronic Acid