Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in cultured fibroblasts.
The ability of beta-carotene, lutein or astaxanthin to protect against UVA-induced oxidative stress in rat kidney fibroblasts (NRK) was assessed. Activities of the antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), and changes in thiobarbituric acid reactive substances (TBARS) were measured as indices of oxidative stress. Exposure to UVA light at a dose intensity of 5.6 mW/cm2 for 4 h resulted in a significant decrease in CAT and SOD activities and a significant increase in TBARS. No cytotoxicity, as indicated by lactate dehydrogenase (LDH) release, was observed. beta-Carotene (1 microM), lutein (1 microM) and astaxanthin (10 nM) protect against UVA light-induced oxidative stress in vitro with astaxanthin exhibiting superior protective properties.
O'Connor
I
I
Department of Nutrition, University College, Cork, Ireland.
O'Brien
N
N
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000528:radiation effects*; D014975:Lutein / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000494:pharmacology*
D000975:Antioxidants; D024341:Xanthophylls; D019207:beta Carotene; C005948:astaxanthine; D002374:Catalase; D013482:Superoxide Dismutase; D014975:Lutein