title: Removal of primate xenoreactive natural antibodies by extracorporeal perfusion of pig kidneys and livers.
abstract: Organ perfusion is one of the possible strategies to attenuate rejection of discordant xenografts by reducing the levels of the recipient's xenoreactive natural antibodies (XNA). Its efficacy in terms of XNA removal was studied in models of primate blood or plasma perfusion through porcine kidneys or livers, with special attention to haematological consequences and potential side-effects. We first perfused the blood of rhesus monkeys through pig kidneys and livers, and demonstrated that the perfusion of a pig liver resulted in higher XNA adsorption (72 +/- 13%) than the perfusion of a pig kidney (51 +/- 25%). However, when we normalized for the weight of the perfused organs and for levels of natural antibodies in individual monkeys, livers adsorbed less antibody (1.4 +/- 0.9 U antibody/g) than kidneys (7.2 +/- 7 U antibody/g). Histological signs of rejection were observed in perfused kidneys, but not in perfused livers. A major drawback of the perfusion of blood through livers was a considerable decrease in the primates' haemoglobin and platelet levels. To avoid this, we developed a plasma liver perfusion device. This method allowed a significant improvement in the haemodynamic state of primates and was particularly effective in preventing anaemia. Moreover, plasma liver perfusion was as effective as blood liver perfusion to remove natural antibodies and, resulted in a marked decrease in their functional activity as assessed by complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC). The level of other plasma proteins was not significantly affected, apart from a dilution effect. After xenoperfusion a strong antibody response was evidenced by ELISA, CDC and ADCC between days 7 and 14 and then decreased progressively. We conclude that the separation of blood to allow the perfusion of plasma through a pig organ is safer than the perfusion of unseparated blood and is associated with efficient natural antibody removal. However, organ perfusion is limited by a rebound in antibody levels after a few days, and thus will have to be associated with anti-B cell immunosuppressive therapy for long-term or repeated applications.
authors.affiliation: Laboratoire de Chirurgie Expérimentale, Fondation Transplantation, Strasbourg, France. fondationtx@mail.sdv.fr
authors.forename: A
authors.initials: A
authors.lastname: Azimzadeh
authors.forename: C
authors.initials: C
authors.lastname: Meyer
authors.forename: H
authors.initials: H
authors.lastname: Watier
authors.forename: J P
authors.initials: JP
authors.lastname: Beller
authors.forename: M P
authors.initials: MP
authors.lastname: Chenard-Neu
authors.forename: R
authors.initials: R
authors.lastname: Kieny
authors.forename: K
authors.initials: K
authors.lastname: Boudjema
authors.forename: D
authors.initials: D
authors.lastname: Jaeck
authors.forename: J
authors.initials: J
authors.lastname: Cinqualbre
authors.forename: P
authors.initials: P
authors.lastname: Wolf
mesh_terms: D000818:Animals; D000910:Antibodies, Heterophile / Q000302:isolation & purification; D000920:Antibody-Dependent Cell Cytotoxicity; D004847:Epithelial Cells; D005112:Extracorporeal Circulation; D006439:Hemodynamics; D007075:Immunoglobulin M / Q000097:blood; D007668:Kidney / Q000276:immunology*; D016030:Kidney Transplantation / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D008253:Macaca mulatta; D010477:Perfusion; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D000910:Antibodies, Heterophile; D007075:Immunoglobulin M