P1,P4-diadenosine 5' tetraphosphate induces nitric oxide release from bovine aortic endothelial cells.
Since the infusion of P1,P4-diadenosine 5' tetraphosphate (Ap4A) into animal models induces vasodilation [1,2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2-fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by NG-nitro-L-arginine (L-NNA) and this inhibition was reversed by L-Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.
Hilderman
R H
RH
Department of Microbiology/Molecular Medicine, Greenville Hospital System/Clemson University Biomedical Cooperative, SC 29634-1903, USA. Hilderr@Clemson.edu
Christensen
E F
EF
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism*; D001120:Arginine / Q000037:antagonists & inhibitors; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D015226:Dinucleoside Phosphates / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism*; D006868:Hydrolysis; D009569:Nitric Oxide / Q000378:metabolism*
D015226:Dinucleoside Phosphates; D009569:Nitric Oxide; C020733:diadenosine tetraphosphate; D001120:Arginine; D002118:Calcium