Human cytomegalovirus infection induces mRNA expression and secretion of plasminogen inhibitor type-1 in endothelial cells.
The aim of the study was to investigate whether infection of endothelial cells with human cytomegalovirus (HCMV) perturbs expression and production of plasminogen activator inhibitor type-1 (PAI-1). mRNA expression of PAI-1 was investigated by isolating total RNA from HCMV-infected and control cells, followed by Northern blotting and probing with 32P-labelled PAI-1 probe. Sandwich ELISA was used to investigate PAI-1 production. HCMV induced the expression of PAI-1-mRNA at 2-5 days postinfection (maximum expression was at 3 days postinfection which was 40% higher than control). HCMV also induced secretion of PAI-1 at 2-5 days postinfection. These results indicate that infection of endothelial cells with HCMV disturbs PAI-1 expression and production in these cells.
Woodroffe
S B
SB
University of Western Sydney (Hawkesbury), Faculty of Science and Technology, New South Wales, Australia. s.woodroffe@uws.edu.au
Kuan
S
S
D015152:Blotting, Northern; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000502:physiology*; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics*
D017395:Plasminogen Activator Inhibitor 1; D012333:RNA, Messenger