title: Mutations at nonliganding residues Tyr-85 and Glu-83 in the N-lobe of human serum transferrin. Functional second shell effects.
abstract: The x-ray crystal structure of the N-lobe of human serum transferrin has shown that there is a hydrogen bond network, the so-called "second shell," around the transferrin iron binding site. Tyrosine at position 85 and glutamic acid at position 83 are two nonliganding residues in this network in the human serum transferrin N-lobe (hTF/2N). Mutation of each of these two amino acids has a profound effect on the metal binding properties of hTF/2N. When Tyr-85 is mutated to phenylalanine, iron release from the resulting mutant Y85F is much more facile than from the parent protein. Elimination of the hydrogen bond between Tyr-85 and Lys-296 appears to interfere with the "di-lysine (Lys-206-Lys-296) trigger," which affects the iron binding stability of the protein. Surprisingly, mutation of Glu-83 to alanine leads to the absence of one of the normal iron binding ligands; introduction of a monovalent anion is able to restore the normal first coordination sphere. The missing ligand appears to be His-249, as revealed by comparison of the metal binding behaviors of mutants H249Q and E83A and structural analysis. Glu-83 has a strong H bond linkage with His-249 in apo-hTF/2N, which helps to hold the His-249 in the proper position for iron binding. Disabling Glu-83 by mutation to an alanine seriously disturbs the H bond network, allowing His-249 to move away. A monovalent anion can help reestablish the normal network by providing a negative charge near the position of Glu-83 to reach charge balance, so that ligand His-249 is available again for iron binding.
authors.affiliation: Department of Biochemistry, College of Medicine, University of Vermont, Burlington, Vermont 05405. qhe@zoo.uvm.edu
authors.forename: Q Y
authors.initials: QY
authors.lastname: He
authors.forename: A B
authors.initials: AB
authors.lastname: Mason
authors.forename: R C
authors.initials: RC
authors.lastname: Woodworth
authors.forename: B M
authors.initials: BM
authors.lastname: Tam
authors.forename: R T
authors.initials: RT
authors.lastname: MacGillivray
authors.forename: J K
authors.initials: JK
authors.lastname: Grady
authors.forename: N D
authors.initials: ND
authors.lastname: Chasteen
mesh_terms: D001483:Base Sequence; D003300:Copper / Q000378:metabolism; D017931:DNA Primers; D004578:Electron Spin Resonance Spectroscopy; D018698:Glutamic Acid / Q000378:metabolism*; D006801:Humans; D006860:Hydrogen Bonding; D007501:Iron / Q000378:metabolism; D008024:Ligands; D009154:Mutation; D011485:Protein Binding; D014168:Transferrin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D008024:Ligands; D014168:Transferrin; D018698:Glutamic Acid; D014443:Tyrosine; D003300:Copper; D007501:Iron