The effect of antioxidant vitamins E and C on lipoperoxidation of erythrocyte membranes during hyperbaric oxygenation.
The aim of this research was to determine whether administration of an antioxidant vitamin combination can reduce oxidative damage in erythrocytes induced by hyperbaric oxygenation (HBO). Malonyldialdehyde (MDA) levels and osmotic fragility ratios in erythrocytes of 28 rats were compared in group A [control], group B [Vitamin (E + C)], group C [HBO] and group D [HBO + Vitamin (E + C)]. HBO was applied at a pressure of 2.8 atmospheres absolute (ATA), 1 hour daily, for 45 days in groups C and D. Administration of alpha-tocopherol acetate (40 mg/kg) and Na-ascorbate (200 mg/kg) was initiated 3 days before the start of HBO exposures and administered intraperitoneally 3 times a week for 45 days. MDA levels and osmotic fragility ratios were significantly higher in group C than in groups A and B (p < 0.05 for all). Significant decreases in MDA levels and osmotic fragility were observed in group D compared with group C, although these parameters were still significantly higher than in controls (p < 0.05 for all). Prolonged HBO resulted in oxidative damage indicated by significant increases in MDA levels and osmotic fragility ratios, which were reduced by concomitant vitamin (E + C) administration.
Etlik
O
O
Department of Physiology, Gülhane Military Medical Academy, Etlik, Ankara, Turkey.
Tomur
A
A
Dündar
K
K
Erdem
A
A
Gündoğan
N U
NU
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism*; D006931:Hyperbaric Oxygenation; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009996:Osmotic Fragility; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D014810:Vitamin E; D008315:Malondialdehyde; D001205:Ascorbic Acid