title: MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways.
abstract: Mucins are high molecular-weight glycoproteins involved in the protection and lubrication of respiratory, gastrointestinal, and reproductive tracts. Hypersecretory diseases such as cystic fibrosis (CF), chronic bronchitis, and asthma result in dysregulated levels of mucin production stemming from increased abundance of mucin-secreting cell types in the surface airway epithelium and submucosal glands. The isolation of at least nine mucin genes has prompted studies to characterize the cellular expression patterns of these mucins in normal and diseased tissues. In the present study, in situ hybridization and immunocytochemical methods were used to determine the cellular distribution of MUC5B and MUC7 expression in CF and non-CF human bronchus. Our findings indicate that MUC5B and MUC7 have expression patterns in human bronchial airways that are limited exclusively to submucosal glands. Specifically, MUC5B expression was confined to all mucous tubules, whereas MUC7 expression was seen in a subset of lysozyme expressing serous tubules of submucosal glands. Interestingly, heterogeneity of MUC7 expression between glands of the same bronchus ranged from 0 to 93% of serous tubules, suggesting that functional diversity may exist between glands within the same bronchial sample. No remarkable differences were observed in the expression patterns of MUC5B or MUC7 between CF (n = 7) and non-CF (n = 10) bronchial samples. In conclusion, MUC5B and MUC7 expressions define different cellular compartments within submucosal glands of human bronchus and lend insight into the heterogeneity of mucin production in the lung.
authors.affiliation: Department of Anatomy and Cell Biology and Department of Internal Medicine at the University of Iowa, Iowa City, Iowa, USA.
authors.forename: P
authors.initials: P
authors.lastname: Sharma
authors.forename: L
authors.initials: L
authors.lastname: Dudus
authors.forename: P A
authors.initials: PA
authors.lastname: Nielsen
authors.forename: H
authors.initials: H
authors.lastname: Clausen
authors.forename: J R
authors.initials: JR
authors.lastname: Yankaskas
authors.forename: M A
authors.initials: MA
authors.lastname: Hollingsworth
authors.forename: J F
authors.initials: JF
authors.lastname: Engelhardt
mesh_terms: D001980:Bronchi / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D003550:Cystic Fibrosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005088:Exocrine Glands / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D055257:Mucin-5B; D009077:Mucins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000235:genetics*
chemical_list: C524578:MUC5B protein, human; C083238:MUC7 protein, human; D055257:Mucin-5B; D009077:Mucins; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides