title: Morphometric analysis of human bone biopsies: a quantitative structural comparison of histological sections and micro-computed tomography.
abstract: As a part of the European Union BIOMED I study "Assessment of Bone Quality in Osteoporosis," the trabecular structure of transiliac bone biopsies was assessed by conventional histomorphometry and by three-dimensional microcomputed tomography (microCT). Sixty-three cylindrical human transiliac bone specimens were obtained post mortem from 27 women and 36 men (age 23-92 years) and evaluated morphometrically in this study. For the conventional histomorphometric analysis, a pair of four undecalcified sections were prepared from two regions in the mediolateral plane of the bone cores. The sections were stained with the von Kossa technique to obtain high-contrast two-dimensional images. Prior to the histologic sectioning the samples were measured with a desktop microCT, providing a 14 microm nominal resolution. The morphometric parameters computed by both methods in two or three dimensions, respectively, were bone volume density (BV/TV), bone surface density (BS/TV), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp). Results showed highly significant (p < 0.0001) correlations between conventional histology and microCT for BV/TV (r = 0.93), BS/TV (r = 0.91), Tb.Th (r = 0.84), and Tb.Sp (r = 0.91). The mean percentage differences between histology and microCT ranged from 2.5% (BV/TV) to 6.1% (Tb.Th). The high correlations and the low differences in terms of absolute numbers between conventional histomorphometric and microtomographic analysis are very promising for the use of micro-tomographic imaging. MicroCT is a nondestructive, fast, and very precise procedure that allows the measurement of cancellous and compact bone in unprocessed biopsies or small bones, as well as a fully automatic determination of three-dimensional morphometric indices.
authors.affiliation: Orthopedic Biomechanics Laboratory, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA. ram@obl.bidmc.harvard.edu
authors.forename: R
authors.initials: R
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Van Campenhout
authors.forename: B
authors.initials: B
authors.lastname: Van Damme
authors.forename: G
authors.initials: G
authors.lastname: Van Der Perre
authors.forename: J
authors.initials: J
authors.lastname: Dequeker
authors.forename: T
authors.initials: T
authors.lastname: Hildebrand
authors.forename: P
authors.initials: P
authors.lastname: Rüegsegger
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D005260:Female; D006652:Histological Techniques; D006801:Humans; D007085:Ilium / Q000000981:diagnostic imaging* / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D008297:Male; D008875:Middle Aged; D008904:Miniaturization; D010024:Osteoporosis / Q000473:pathology; D014057:Tomography, X-Ray Computed / Q000295:instrumentation / Q000379:methods*