title: Developmental expression of nitric oxide synthase in the rat diencephalon with special reference to the thalamic paratenial nucleus.
abstract: Using immunohistochemistry and in situ hybridization the distribution of nitric oxide synthase (NOS) was investigated in the rat brain during pre- and postnatal development. At E15 weak NOS-like immunoreactivity (NOS-LI) could be seen in the differentiation field of the anterior hypothalamus. At E17 strong NOS-LI was observed in the developing neurons of the hypothalamic paraventricular nucleus, supraoptic nucleus, anterodorsal nucleus and lateral hypothalamic areas. In the thalamic paratenial nucleus a strong NOS-LI was observed in these neurons at E17, E18 and P1 with a weaker intensity at P3, P7, P9 and P15, whereas at P30 and in adult rats no NOS-positive neurons could be detected. NOS expression at E17 and P3 was verified by in situ hybridization. These results suggest that NO may have a developmental role at least in one of the regions studied, the thalamic paratenial nucleus.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
authors.forename: O
authors.initials: O
authors.lastname: Gorbatyuk
authors.forename: M
authors.initials: M
authors.lastname: Landry
authors.forename: P
authors.initials: P
authors.lastname: Emson
authors.forename: I
authors.initials: I
authors.lastname: Akmayev
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism*; D004027:Diencephalon / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development; D005314:Embryonic and Fetal Development / Q000502:physiology; D005333:Fetus / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D019001:Nitric Oxide Synthase / Q000235:genetics / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats / Q000196:embryology / Q000254:growth & development; D017207:Rats, Sprague-Dawley; D013787:Thalamic Nuclei / Q000196:embryology / Q000201:enzymology* / Q000254:growth & development
chemical_list: D012333:RNA, Messenger; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat