A general RNA-binding protein complex that includes the cytoskeleton-associated protein MAP 1A.
Association of mRNA with the cytoskeleton represents a fundamental aspect of RNA physiology likely involved in mRNA transport, anchoring, translation, and turnover. We report the initial characterization of a protein complex that binds RNA in a sequence-independent but size-dependent manner in vitro. The complex includes a approximately 160-kDa protein that is bound directly to mRNA and that appears to be either identical or highly related to a approximately 1600-kDa protein that binds directly to mRNA in vivo. In addition, the microtubule-associated protein, MAP 1A, a cytoskeletal associated protein is a component of this complex. We suggest that the general attachment of mRNA to the cytoskeleton may be mediated, in part, through the formation of this ribonucleoprotein complex.
DeFranco
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Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Chicurel
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Potter
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D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002522:Chlorocebus aethiops; D003599:Cytoskeleton / Q000378:metabolism* / Q000528:radiation effects; D046911:Macromolecular Substances; D008869:Microtubule-Associated Proteins / Q000378:metabolism* / Q000528:radiation effects; D008970:Molecular Weight; D016716:PC12 Cells; D011485:Protein Binding / Q000528:radiation effects; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism* / Q000528:radiation effects; D051381:Rats; D012697:Serine Endopeptidases / Q000378:metabolism; D014466:Ultraviolet Rays
D046911:Macromolecular Substances; C490546:Map1a protein, rat; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012697:Serine Endopeptidases; C028344:glutamyl endopeptidase