title: Polyethylene glycol-induced hypovolemia affects the expression of MCH mRNA, but not dynorphin or secretogranin II mRNAs, in the rat lateral hypothalamus.
abstract: Two prominent neuron populations of the rat lateral hypothalamus express genes encoding respectively the prepromelanin-concentrating hormone (MCH) or dynorphin (DYN) and secretogranin II (SGII). Their roles remain hypothetical in mammals. In the present study, we examined the changes in MCH, DYN and SGII gene expression in dehydrated rats compared to controls. Dehydration was obtained by subcutaneous injection of polyethylene glycol (PEG) resulting in a large reduction of the extracellular fluid volume. Using competitive semi-quantitative RT-PCR and in situ hybridization methods, PEG-injected animals showed a significant increase of MCH mRNA level but no variation of DYN and SGII mRNA levels. These results confirm previous observations suggesting that intra- and extracellular dehydration challenges affect different regulation circuits; they indicate that both neuron populations could be involved in the maintenance of body fluid homeostasis, directly, or indirectly, as integrators of various information leading to goal-oriented behaviour.
authors.affiliation: CNRS UPRESA 6025, Laboratoire d'Histologie Embryologie Cytogénétique, Faculté de Médecine, Besançon, France.
authors.forename: C
authors.initials: C
authors.lastname: Hervé
authors.forename: C
authors.initials: C
authors.lastname: Colard
authors.forename: S
authors.initials: S
authors.lastname: Grillon
authors.forename: D
authors.initials: D
authors.lastname: Fellmann
authors.forename: B
authors.initials: B
authors.lastname: Griffond
mesh_terms: D000818:Animals; D002864:Chromogranins; D004399:Dynorphins / Q000096:biosynthesis* / Q000235:genetics; D005110:Extracellular Space / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation; D007028:Hypothalamic Hormones / Q000096:biosynthesis* / Q000235:genetics; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D008543:Melanins / Q000096:biosynthesis* / Q000235:genetics; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D010907:Pituitary Hormones / Q000096:biosynthesis* / Q000235:genetics; D011092:Polyethylene Glycols / Q000633:toxicity*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D002864:Chromogranins; D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D011506:Proteins; D012333:RNA, Messenger; D011092:Polyethylene Glycols; C019463:melanin-concentrating hormone; D004399:Dynorphins