title: A family of flavoproteins in the domains Archaea and Bacteria.
abstract: A family of flavoproteins, called A-type flavoproteins, is described. It consists of 14 protein sequences of 385-597 amino acids in length, 7 from methanogens (domain: Archaea), 5 from phototrophic prokaryotes, one from Escherichia coli, and a partial sequence from the sulfate reducer Desulfovibrio gigas (domain: Bacteria). No similar sequence could be found in the domain Eucarya. All sequences show significant similarity over a 385-400 amino acid portion overlapping a recognizable flavodoxin signature starting at positions 245-285 of the common core sequence. Cofactor analysis and, to some extent, analysis of the primary structure of six A-type flavoproteins, three of which are structurally characterized here, support the existence of four sub-families: (a) simple flavoproteins binding only FMN; (b) diflavin flavoproteins binding FMN and FAD; (c) a flavorubredoxin binding FMN and iron; (d) a hemoflavoprotein. The possible involvement of A-type flavoproteins in the metabolism of oxygen, as suggested for D. gigas hemoflavoprotein [Gomes, C. M., Silva, G., Oliveira, S., LeGall, J., Liu, M.-Y., Xavier, A. V., Rodrigues-Pousada, C. & Teixeira, M. (1997) J. Biol. Chem. 272, 22502-22508], is discussed.
authors.affiliation: Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland. wasserfallen@micro.biol.ethz.ch
authors.forename: A
authors.initials: A
authors.lastname: Wasserfallen
authors.forename: S
authors.initials: S
authors.lastname: Ragettli
authors.forename: Y
authors.initials: Y
authors.lastname: Jouanneau
authors.forename: T
authors.initials: T
authors.lastname: Leisinger
mesh_terms: D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D019641:DNA, Archaeal / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D003901:Desulfovibrio / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005420:Flavoproteins / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D006899:Mixed Function Oxygenases / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D016354:Rhodobacter capsulatus / Q000235:genetics; D012416:Rubredoxins / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D019843:Archaeal Proteins; D001426:Bacterial Proteins; D017931:DNA Primers; D019641:DNA, Archaeal; D004269:DNA, Bacterial; D005420:Flavoproteins; D011994:Recombinant Proteins; D012416:Rubredoxins; D006899:Mixed Function Oxygenases; C072680:nitrilotriacetate monooxygenase