The intracellular domain of the rabbit prolactin receptor is able to promote the secretion of a passenger protein via an unusual secretory pathway in lepidopteran cells.
We have previously shown that the intracellular domain of the rabbit prolactin receptor (rbPRL-R), lacking typical signal sequences, was very efficiently secreted into the culture medium when expressed in the baculovirus-insect cell system. We have sought to take advantage of this characteristic for secreting cytoplasmic or nuclear proteins. We have constructed a series of recombinant viruses expressing a foreign gene product fused to the intracellular domain of rbPRL-R. Two passenger genes were used, one encoding a cytoplasmic protein (cyclin B) and the other a nuclear protein (cyclin A). The intracellular domain of rbPRL-R was able to promote the export of these two chimeric proteins with a very high efficiency. This new system should prove useful for secretion of proteins which do not require the post-translational modifications of the classical secretory pathway to be fully active.
Garnier
L
L
Laboratoire de Pathologie Comparée, Centre National de la Recherche Scientifique URA 1184, Saint-Christol-les-Alès, France. garnier@cor.mail.biochem.hmc.psu.edu
Cahoreau
C
C
Devauchelle
G
G
Cérutti
M
M
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D017077:Culture Media, Conditioned; D019925:Cyclin A / Q000235:genetics / Q000378:metabolism*; D019926:Cyclin B / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D017353:Gene Deletion; D015870:Gene Expression; D005822:Genetic Vectors; D008854:Microscopy, Electron; D021382:Protein Sorting Signals / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D011817:Rabbits; D011981:Receptors, Prolactin / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D011993:Recombinant Fusion Proteins; D018411:Spodoptera / Q000378:metabolism / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D017077:Culture Media, Conditioned; D019925:Cyclin A; D019926:Cyclin B; D021382:Protein Sorting Signals; D011981:Receptors, Prolactin; D011993:Recombinant Fusion Proteins