Identification of adenylyl cyclase isoenzymes in CHO and B82 cells.
The identification of adenylyl cyclase isoenzymes in mammalian host cells is important for the interpretation of data obtained from cell lines heterologously expressing G-protein coupled receptors. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify adenylyl cyclase cDNAs from Chinese hamster ovary (CHO) and mouse fibroblast (B82) cells. The isolated fragments were identified by restriction analyses and by sequencing. We found mRNAs for adenylyl cyclases VI and VII in CHO and adenylyl cyclases IX and VII in B82 cells.
Varga
E V
EV
Department of Pharmacology, College of Medicine, The University of Arizona Health Sciences Center, Tucson 85724, USA.
Stropova
D
D
Rubenzik
M
M
Wang
M
M
Landsman
R S
RS
Roeske
W R
WR
Yamamura
H I
HI
D000262:Adenylyl Cyclases / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D019204:GTP-Binding Proteins / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D015183:Restriction Mapping
D007527:Isoenzymes; D012333:RNA, Messenger; D019204:GTP-Binding Proteins; D000262:Adenylyl Cyclases