title: On the intrinsic mechanisms of spontaneous motility in chick embryos.
abstract: Our experimental study was aimed on the problems of co-operation of NMDA- and NO-ergic mechanisms in the genesis of spontaneous motility in 17-day-old chick embryos. 1. Blockade of NO-synthase (NOS) by 7-nitro indazole prevented effect of an activation cocktail (NMDA + L-arginine). 2. Simultaneous blockade of NOS and of polyamine site in NMDA-receptor by arcaine was revealed by increased NMDA-activation of the spontaneous motility and by blockade of L-arginine and cocktail effects. 3. The blockade of NMDA-activation, depression of L-arginine activation and short-lasting potentiation of cocktail effect. 4. Blockade of NMDA mechanism by Mg2+ abolished activatory effect of both NMDA and L-arginine. The initial activation remained from the cocktail effect only. Results were considered as an evidence of a possible co-operation among NMDA- and NO-ergic mechanisms in the development of the embryonic spontaneous motility.
authors.affiliation: Institute of Physiology, 1st faculty of Medicine, Charles University, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Sedlácek
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002642:Chick Embryo / Q000502:physiology*; D009068:Movement / Q000187:drug effects; D016202:N-Methylaspartate / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology; D009569:Nitric Oxide / Q000494:pharmacology / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000502:physiology
chemical_list: D009569:Nitric Oxide; D016202:N-Methylaspartate; D001120:Arginine; D019001:Nitric Oxide Synthase