Thermal analysis of bones from ovariectomized rats.
Thermal analyses [thermogravimetry (TG) and differential thermal analysis (DTA)], X-ray diffraction, and infrared absorption analysis of bones from ovariectomized rats were carried out. The rats were divided into five groups: sham operated (Sham); ovariectomized (OVX); OVX given traditional Chinese (Kampo) medicine, Unkei-to; OVX given 17 beta-estradiol; and OVX given the estradiol vehicle, respectively. The activation energy (delta E), a kinetic parameter from TG data of OVX rats, increased by 57% from that in Sham rats. The administration of Unkei-to and 17 beta-estradiol to OVX rats clearly restored the delta E to the levels of Sham rats, while the vehicle for 17 beta-estradiol had no effect. DTA data from thermal analyses of rats from the Sham, OVX, and OVX given various compounds were almost the same except for OVX rats given 17 beta-estradiol. The X-ray diffraction pattern and infrared absorption spectrum of bone powders from Sham rats were not different from those of OVX rats or others. These results strongly suggest that kinetic parameter, delta E calculated from TG data, may be a useful method for assessing both experimentally induced osteoporosis and drug effects on it.
Okamoto
Y
Y
Department of Dental Materials, Fukuoka Dental College, Japan.
Hidaka
S
S
Yamada
Y
Y
Ouchi
K
K
Miyazaki
K
K
Liu
S Y
SY
D000818:Animals; D001842:Bone and Bones / Q000473:pathology*; D002254:Carbonates / Q000032:analysis; D004055:Differential Thermal Analysis; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D004958:Estradiol / Q000627:therapeutic use; D015914:Estrogen Replacement Therapy; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008516:Medicine, Chinese Traditional; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy / Q000473:pathology*; D010052:Ovariectomy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013055:Spectrophotometry, Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
D002254:Carbonates; D004365:Drugs, Chinese Herbal; C058415:unkei-to; D004958:Estradiol