title: Human natural resistance-associated macrophage protein is a new type of microtubule-associated protein.
abstract: Natural resistance-associated macrophage protein 1 (NRAMP1) is a putative membrane protein that dominates natural resistance to infection. An NRAMP1-glutathione S-transferase fusion protein was used to test the ability of the NRAMP1 NH2-terminal domain to bind to taxol-stabilized microtubules. Co-sedimentation analysis showed that the fusion protein binds to microtubules. Although the NH2-terminal domain of the NRAMP1 molecule has structural homology with the Pro-rich region of microtubule-associated protein 4 (MAP4), the presence of the MAP4 microtubule-binding domain fragment had little effect on the binding of the fusion protein to microtubules.
authors.affiliation: Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineering, Kyushu Institute of Technology, Fukuoka, Japan. dc9603@bse.kyutech.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Tokuraku
authors.forename: H
authors.initials: H
authors.lastname: Nakagawa
authors.forename: F
authors.initials: F
authors.lastname: Kishi
authors.forename: S
authors.initials: S
authors.lastname: Kotani
mesh_terms: D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D027682:Cation Transport Proteins; D002417:Cattle; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007113:Immunity, Innate; D008264:Macrophages / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D005982:Glutathione Transferase; D008565:Membrane Proteins; D008869:Microtubule-Associated Proteins; D017239:Paclitaxel; D011993:Recombinant Fusion Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1