Maternal and viral factors in vertical transmission of HIV-1 subtype E.
Vertical transmission of HIV-1 is caused by multifactorial factors. To access the relationship of viral factors involving in perinatal transmission of HIV-1 subtype E, which is the predominant type in Thailand, plasma viral load, blood CD4+ lymphocyte level, heteroduplex mobility, and V3 sequence of the HIV-1 envelope gene were studied in 32 transmitting and 25 non-transmitting mothers. We found that HIV-1 subtype E vertical transmission was strongly associated with high maternal plasma viral RNA (> 4 x 10(4) copies/ml) and high genetic diversity of envelope gene determined by heteroduplex mobility (< 0.9). The variation of nucleotide sequences in envelope gene of subtype E vertical transmission could not determine in V3 region. Hence, plasma viral load and heteroduplex mobility can be used as prediction factors in vertical transmission of HIV-1 subtype E.
Sutthent
R
R
Department of Microbiology, Mahidol University, Bangkok, Thailand.
Foongladda
S
S
Chearskul
S
S
Wanaprapa
N
N
Likanonskul
S
S
Kositanont
U
U
Riengrojpitak
S
S
Sahapong
S
S
Wasi
C
C
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D017931:DNA Primers; D005260:Female; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012367:RNA, Viral / Q000097:blood; D013785:Thailand; D019562:Viral Load
D017931:DNA Primers; D012367:RNA, Viral