Increased Fas-mediated cytotoxicity of CD4-positive T cells in patients with human T-lymphotropic virus type I-associated myelopathy.
Using a 51Cr release assay, we investigated Fas-mediated cytotoxicity of peripheral blood CD4+ T cells of patients with human T-lymphotropic virus type-I (HTLV-I)-associated myelopathy (HAM) against T98G, a glioblastoma cell line which expresses Fas. Cytotoxic activity of CD4+ T cells against T98G was significantly higher in HAM patients than in controls. Moreover, when CD4+ T cells of HAM patients were preincubated with a monoclonal antibody to human Fas ligand (FasL), cytotoxic activity against T98G was significantly suppressed. These results suggest that damage to nervous tissues by the Fas/FasL system is involved in the pathogenesis of HAM.
Nishiura
Y
Y
First Department of Internal Medicine, Nagasaki University School of Medicine, Sakamoto, Japan.
Furuya
T
T
Nakamura
T
T
Kawakami
A
A
Tsuboi
M
M
Nakane
S
S
Shirabe
S
S
Nakashima
T
T
Fujiyama
K
K
Eguchi
K
K
D000368:Aged; D000911:Antibodies, Monoclonal; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry / Q000276:immunology* / Q000821:virology*; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005909:Glioblastoma; D006801:Humans; D007075:Immunoglobulin M; D008875:Middle Aged; D015493:Paraparesis, Tropical Spastic / Q000276:immunology* / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D019014:fas Receptor / Q000276:immunology* / Q000378:metabolism
D000911:Antibodies, Monoclonal; D002860:Chromium Radioisotopes; D007075:Immunoglobulin M; D019014:fas Receptor