Epstein-Barr virus-induced molecule 1 ligand chemokine is expressed by dendritic cells in lymphoid tissues and strongly attracts naive T cells and activated B cells.
Movement of T and B lymphocytes through secondary lymphoid tissues is likely to involve multiple cues that help the cells navigate to appropriate compartments. Epstein-Barr virus- induced molecule 1 (EBI-1) ligand chemokine (ELC/MIP3beta) is expressed constitutively within lymphoid tissues and may act as such a guidance cue. Here, we have isolated mouse ELC and characterized its expression pattern and chemotactic properties. ELC is expressed constitutively in dendritic cells within the T cell zone of secondary lymphoid tissues. Recombinant ELC was strongly chemotactic for naive (L-selectinhi) CD4 T cells and for CD8 T cells and weakly attractive for resting B cells and memory (L-selectinlo) CD4 T cells. After activation through the B cell receptor, the chemotactic response of B cells was enhanced. Like its human counterpart, murine ELC stimulated cells transfected with EBI-1/CC chemokine receptor 7 (CCR7). Our findings suggest a central role for ELC in promoting encounters between recirculating T cells and dendritic cells and in the migration of activated B cells into the T zone of secondary lymphoid tissues.
Ngo
V N
VN
Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, California 94143-0414, USA.
Tang
H L
HL
Cyster
J G
JG
D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology; D054415:Chemokine CCL19; D019742:Chemokines, CC / Q000502:physiology*; D002633:Chemotaxis / Q000502:physiology; D003713:Dendritic Cells / Q000378:metabolism; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D010581:Peyer's Patches / Q000166:cytology; D012333:RNA, Messenger / Q000378:metabolism; D054400:Receptors, CCR7; D011956:Receptors, Cell Surface / Q000235:genetics; D019707:Receptors, Chemokine; D016415:Sequence Alignment; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000378:metabolism*; D014162:Transfection / Q000235:genetics
C515460:CCL19 protein, human; C515370:CCR7 protein, human; C515461:Ccl19 protein, mouse; C515371:Ccr7 protein, mouse; D054415:Chemokine CCL19; D019742:Chemokines, CC; D012333:RNA, Messenger; D054400:Receptors, CCR7; D011956:Receptors, Cell Surface; D019707:Receptors, Chemokine; D002118:Calcium