[Effects of herpes simplex virus thymidine kinase gene transduction and prodrug on ovarian cancer cell].
OBJECTIVE
To evaluate the efficacy of gene therapy with the herpes simplex virus thymidine kinase (HSV-TK) gene transference followed by ganciclovir (GCV) therapy in ovarian cancer.


METHODS
HSV-TK gene was inserted into the hind III site of pLNSX vector and recombinant plasmid (pLNS/HSV-TK) was constracted. Then, the recombinant plasmid was transfered into PA317 packaged cell and the producer cell line of recombinant retrovirus vector pLNS/HSV-TK was established. The cytotoxicity efficacy of HSV-TK/GCV system to AO cell line was evaluated by microcucture tetrajolium test (MTT) method.


RESULTS
The recombinant retroviral vector pLNS/HSK-TK can transfer HSV-SK gene into the genome of AO cell line and make it sensitive to GCV. The growth inhibitory rate of AO cell line transfered by pLNS/HSV-TK was 98.0% in the medium containing 400 mumol GVC.


CONCLUSIONS
The ovarian cancer cell line transfered by HSV-TK gene regressed rapidly in response to the GCV therapy.
Liang
Z
Z
Sun Yetsan Hospital, Zhongshan Medical University, Guangzhou.
Kuang
J
J
Lin
Z
Z
D000998:Antiviral Agents / Q000494:pharmacology*; D004251:DNA Transposable Elements; D005260:Female; D015774:Ganciclovir / Q000494:pharmacology*; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*; D010957:Plasmids / Q000235:genetics; D011355:Prodrugs / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured
D000998:Antiviral Agents; D004251:DNA Transposable Elements; D011355:Prodrugs; D011994:Recombinant Proteins; D013937:Thymidine Kinase; D015774:Ganciclovir