The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway.
The 11-cis-retinol dehydrogenase (11-cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11-cis-retinol oxidation in the visual cycle. The structure of the murine 11-cis-RoDH gene was used to reinvestigate its transcription pattern. An 11-cis-RoDH gene transcript was detected in several non-ocular tissues. The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11-cis-RoDH was found capable of oxidizing and reducing 9-cis-, 11-cis- and 13-cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-beta-1-maltoside used to solubilize the enzyme was found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed.
Driessen
C A
CA
Department of Ophthalmology, University of Nijmegen, The Netherlands. c.driessen@ohk.azn.nl
Winkens
H J
HJ
Kuhlmann
E D
ED
Janssen
A P
AP
van Vugt
A H
AH
Deutman
A F
AF
Janssen
J J
JJ
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000077556:Alitretinoin; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D003315:Cornea / Q000201:enzymology*; D003360:Cosmids; D005091:Exons; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D018411:Spodoptera; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism*; D014785:Vision, Ocular
D011994:Recombinant Proteins; D000077556:Alitretinoin; D014212:Tretinoin; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase