High-Na+ low-K+ UW cold storage solution reduces reperfusion injuries of the rat liver graft.
The isolated perfused rat liver model was used to assess graft viability after 24 h of cold preservation. Two solutions were compared for liver preservation: Belzer's original UW solution (high-K+ UW) and a solution containing the same components but with inverted concentrations of sodium and potassium (high-Na+ UW). During the 120 min of normothermic reperfusion, livers preserved in the high-Na+ UW solution released lower levels of creatine kinase-BB isoenzyme, transaminases (ALT and AST), and potassium than those preserved in the high-K+ UW solution. Bile flow and biliary excretion of indocyanine green increased when livers were preserved in the high-Na+ UW solution. We found no statistical differences for oxygen consumption and tissue ATP concentration. The results of this study support the concept that a high-Na+ UW solution is a more effective means of preserving rat livers, at least after 24 h of cold-storage and 120 min of reperfusion in the isolated perfused model, than the original high-K+ UW solution. Liver preservation in the high-Na+ UW solution reduces damage to sinusoidal endothelial and hepatocellular cells. The use of an extracellular-like Belzer cold storage solution eliminates potassium-related problems in cold preservation and subsequent normothermic reperfusion while keeping all the qualities of the original UW solution.
Ben Abdennebi
H
H
Laboratoire de Recherches Chirurgicales, INSERM U. 281, Hôpital E. Herriot, Lyon, France.
Steghens
J P
JP
Margonari
J
J
Ramella-Virieux
S
S
Barbieux
A
A
Boillot
O
O
D000241:Adenosine; D000410:Alanine Transaminase / Q000378:metabolism; D000493:Allopurinol; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D015925:Cryopreservation; D005978:Glutathione; D007208:Indocyanine Green / Q000493:pharmacokinetics; D007328:Insulin; D007527:Isoenzymes; D008099:Liver / Q000201:enzymology; D008102:Liver Circulation; D016031:Liver Transplantation / Q000379:methods*; D008297:Male; D008833:Microcirculation; D019188:Organ Preservation Solutions; D011188:Potassium; D011887:Raffinose; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000517:prevention & control*; D012964:Sodium
D007328:Insulin; D007527:Isoenzymes; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D012964:Sodium; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D003402:Creatine Kinase; D005978:Glutathione; D007208:Indocyanine Green; D000241:Adenosine; D011887:Raffinose; D011188:Potassium