Characterization of a novel liver-specific protein/DNA binding site in the human HMG CoA reductase promoter.
These studies define a novel binding element (site C) within the human HMG CoA reductase promoter using a combination of in vitro DNase I footprinting and gel mobility shift assays. The factor interacting with site C appears to be restricted to the liver, indicating a possible role for this protein in regulating hepatic expression of the gene. Studies based on competitive gel mobility shift assays and transient co-transfection experiments performed using a reporter construct harbouring the promoter of HMG CoA reductase suggest that the protein binding to site C may belong to the C/EBP family of transcription factors. A factor interacting with this binding element was also identified in human liver nuclear protein extracts.
Gauthier
B R
BR
Cancer Research Laboratories and Department of Biochemistry, Queen's University, Kingston, Ontario, K7L 3N6, Canada. bgauthie@heartinst.on.ca
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D006224:Cricetinae; D004247:DNA / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D008099:Liver / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection
D014157:Transcription Factors; D004247:DNA; D006903:Hydroxymethylglutaryl CoA Reductases