Cloning of a novel C-type lectin expressed by murine macrophages.
We report the cloning of a novel macrophage-restricted C-type lectin by differential display polymerase chain reaction. This molecule, named mouse macrophage C-type lectin, is a 219-amino acid, type II transmembrane protein with a single extracellular C-type lectin domain. Northern blot analysis indicates that it is expressed in cell lines and normal mouse tissues in a macrophage-restricted manner. The cDNA and genomic sequences of mouse macrophage C-type lectin indicate that it is related to the Group II animal C-type lectins. The mcl gene locus has been mapped between the genes for the interleukin-17 receptor and CD4 on mouse chromosome 6, the same chromosome as the mouse natural killer cell gene complex.
Balch
S G
SG
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. sjoh0078@sable.ox.ac.uk
McKnight
A J
AJ
Seldin
M F
MF
Gordon
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D019556:COS Cells; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D006224:Cricetinae; D037102:Lectins / Q000235:genetics*; D037181:Lectins, C-Type; D008264:Macrophages / Q000737:chemistry*; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
C113583:Clecsf8 protein, mouse; D037102:Lectins; D037181:Lectins, C-Type; D008565:Membrane Proteins