Development of normal mice from oocytes injected with freeze-dried spermatozoa.
Freeze-dried mouse spermatozoa are all motionless and dead in the conventional sense. When injected into oocytes, however, their nuclei can support normal embryonic development even after three month preservation in a dried state. Although the freeze-drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genomes at room temperature.
Wakayama
T
T
Department of Anatomy, University of Hawaii School of Medicine, Honolulu.
Yanagimachi
R
R
D000822:Animal Husbandry / Q000379:methods*; D000818:Animals; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D005612:Freeze Drying / Q000379:methods*; D008098:Litter Size / Q000502:physiology; D008297:Male; D051379:Mice; D009865:Oocytes / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*