tetA(L) mutants of a tetracycline-sensitive strain of Bacillus subtilis with the polynucleotide phosphorylase gene deleted.
A Bacillus subtilis strain with the polynucleotide phosphorylase gene deleted was sensitive for growth in the presence of tetracycline. This strain was used to select for tetracycline-resistant mutants. A point mutation in the tetA(L) promoter and a spontaneously occurring tetA(L) gene copy number mutant were characterized.
Bechhofer
D H
DH
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, New York 10029, USA. dbechho@smtplink.mssm.edu
Stasinopoulos
S J
SJ
D017920:Antiporters / Q000235:genetics*; D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D017353:Gene Deletion; D008969:Molecular Sequence Data; D017354:Point Mutation; D011117:Polyribonucleotide Nucleotidyltransferase / Q000235:genetics*; D011401:Promoter Regions, Genetic; D013752:Tetracycline / Q000494:pharmacology*; D013753:Tetracycline Resistance / Q000235:genetics*
D017920:Antiporters; D001426:Bacterial Proteins; C079051:tetA protein, Bacteria; D011117:Polyribonucleotide Nucleotidyltransferase; D013752:Tetracycline