title: Immunoglobulin superfamily members gp65 and gp55: tissue distribution of glycoforms.
abstract: Gp65 and gp55 are immunoglobulin superfamily members produced by alternative splicing of the same gene transcript, and originally identified as components of synaptic membranes. A monoclonal antibody specific for gp65 and gp55 has been used to detect immunoreactive species in a wide range of tissues. All immunoreactive species bind to concanavalin A and deglycosylation studies show that in all tissues tested other than brain the immunoreactive species are derived from gp55. HEK cells transfected with gp65 or gp55 express different glycoforms from brain showing that the pattern of glycosylation of these molecules is dependent upon the cell type in which they are expressed.
authors.affiliation: Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Surrey, UK.
authors.forename: K
authors.initials: K
authors.lastname: Langnaese
authors.forename: R
authors.initials: R
authors.lastname: Mummery
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: P W
authors.initials: PW
authors.lastname: Beesley
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D006031:Glycosylation; D007136:Immunoglobulins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000302:isolation & purification*; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C548934:Nptn protein, rat; C103658:synaptic glycoprotein gp65