title: Scanning electron microscopy of the root surface texture of extracted periodontally diseased teeth following various etching and chelating regimens.
abstract: Scanning electron microscopy of root surfaces that had been ultrasonically scaled and subjected to various conditioning regimens revealed the presence of two distinct types of cracks: extensive cracks, presumed to have been caused by drying before and during sputter-coating procedures; and smaller cracks that reflected the pattern of the irregular underlying dentin. Both etching and chelating agents appear to cause demineralization of the interfacial layer between cementum and dentin, causing a "peeling off" of cementum and exposure of the underlying dentin. The results suggest that burnishing the scaled root surface with either saline or any of the etching or chelating agents for at least 10 seconds, followed by soaking the cementum in 8% ethylenediaminetetraacetic acid for about 40 seconds, achieved a root surface that might be regarded as optimal for regeneration of periodontal tissues.
authors.affiliation: Department of Preventive Dental Sciences, College of Dentistry, King Saud University College of Dentistry, Riyadh, Kingdom of Saudi Arabia.
authors.forename: A
authors.initials: A
authors.lastname: Bergenholtz
authors.forename: N
authors.initials: N
authors.lastname: Babay
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods*; D002614:Chelating Agents / Q000494:pharmacology; D019343:Citric Acid / Q000494:pharmacology; D003094:Collagen; D003739:Dental Cementum / Q000187:drug effects / Q000648:ultrastructure; D003804:Dentin / Q000187:drug effects / Q000648:ultrastructure; D004492:Edetic Acid / Q000494:pharmacology; D016557:Guided Tissue Regeneration, Periodontal; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010347:Patient Care Planning; D016369:Smear Layer; D012965:Sodium Chloride / Q000494:pharmacology; D013499:Surface Properties / Q000187:drug effects; D013752:Tetracycline / Q000494:pharmacology; D013997:Time Factors; D014092:Tooth Root / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D002614:Chelating Agents; D019343:Citric Acid; D012965:Sodium Chloride; D003094:Collagen; D004492:Edetic Acid; D013752:Tetracycline