title: Interim storage of avulsed permanent teeth.
abstract: This literature review examines the effects of extra-alveolar duration and storage conditions on the healing of the periodontal ligament (PL) of avulsed teeth following replantation. A critical assessment of the so-called "reconstitution theory" for PL cells is also presented. The ideal treatment for an avulsed tooth is immediate replantation (i.e. less than five minutes). Unfortunately, immediate replantation is not the norm, and most avulsed teeth experience delayed replantation. The greatest risk to the vitality of the PL cells attached to the root of an avulsed tooth is desiccation. Not surprisingly, storage media affect PL healing. Storing avulsed teeth in an isotonic liquid is superior to dry storage. Numerous studies support the use of Modified Eagle's Medium, Hank's Balanced Salt Solution (HBSS), ViaSpan, saline, milk, and saliva as storage media. A recent study has suggested that chicken egg white may also prove to be a suitable storage medium for avulsed teeth. Of these media, only saliva is always present at the scene of an accident, however. Milk can usually be obtained on short notice, but even 10 minutes of desiccation can affect the outcome of replantation. From a practical standpoint, milk packed in ice seems to be the best alternative for the temporary storage of avulsed teeth, due to its wide availability and the minimal detrimental effects it has on PL cells. There is no evidence to support the suggestion that HBSS or any other media will "reconstitute" the PL cells when the extra-alveolar duration of avulsed teeth is greater than 30 minutes.
authors.affiliation: Hospital for Sick Children, Toronto, Ont.
authors.forename: M L
authors.initials: ML
authors.lastname: Layug
authors.forename: E J
authors.initials: EJ
authors.lastname: Barrett
authors.forename: D J
authors.initials: DJ
authors.lastname: Kenny
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D003890:Desiccation; D005978:Glutathione; D006801:Humans; D007328:Insulin; D007552:Isotonic Solutions; D008892:Milk; D019188:Organ Preservation Solutions; D010513:Periodontal Ligament / Q000166:cytology; D011887:Raffinose; D012463:Saliva; D012965:Sodium Chloride; D014021:Tissue Preservation; D014084:Tooth Avulsion; D014090:Tooth Replantation; D014867:Water
chemical_list: C072670:Hanks Balanced Salt Solution; D007328:Insulin; D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D014867:Water; D012965:Sodium Chloride; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose