[Riboflavin Reference Standard (Control 951) of National Institute of Health Sciences].
The raw material for riboflavin was tested for preparation of the "Riboflavin Reference Standard (Control 951)" of National Institute of Health Sciences. Analytical data obtained were as follows: melting point, 284.6 degrees C (decomposition): specific absorbance, E1cm1% = 857 (267 nm), 277 (373 nm), 326 (445 nm); IR spectrum, the same as that of JP Riboflavin Reference Standard (Control 921); optical rotation, [alpha]20D = -135.6 degrees; thin-layer chromatography, three impurities were detected; high-performance liquid chromatography, a small amount of 10 impurities were detected: loss on drying, 0.10%; assay, 100.4% by spectrophotometry. Based on the above results, the raw material was authorized as the Riboflavin Reference Standard (Control 951) of National Institute of Health Sciences (Japanese Pharmacopoeia).
Kitajima
A
A
Tagashira
Y
Y
Maekawa
K
K
Yoshii
K
K
Komatsu
H
H
Tanimoto
T
T
Okada
S
S
D007564:Japan; D012015:Reference Standards; D012256:Riboflavin / Q000592:standards*
D012256:Riboflavin