title: Role of the actin cytoskeleton in regulating the outer phosphatidylethanolamine levels in yeast plasma membrane.
abstract: Transbilayer phosphatidylethanolamine (PtdEtn) movements in the plasma membrane of Saccharomyces cerevisiae are regulated by an ATP-dependent, protein-mediated process(es). To examine whether this process is influenced by the actin cytoskeleton, we have studied the PtdEtn translocation in S. cerevisiae cells after treatment with microfilament disrupting and microtubule-disrupting agents. PtdEtn translocation was studied by measuring the external PtdEtn levels, using fluorescamine as the external membrane probe, in the ATP-depleted, ATP-depleted and repleted, and N-ethylmaleimide-treated cells. The microfilaments and microtubules were disrupted by treatment with various cytochalasins and colchicine (or benomyl) respectively PtdEtn translocation became abnormal in the cytochalasin-treated cells but not in cells that were treated with microtubule-disrupting agents, such as colchicine or benomyl. These results have been interpreted to suggest that the actin cytoskeleton is involved in regulating the PtdEtn translocase activity in the yeast cell plasma membrane.
authors.affiliation: Division of Membrane Biology, Central Drug Research Institute, Lucknow, India.
authors.forename: B L
authors.initials: BL
authors.lastname: Dixit
authors.forename: C M
authors.initials: CM
authors.lastname: Gupta
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects; D000199:Actins / Q000502:physiology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001542:Benomyl / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D005033:Ethylmaleimide / Q000494:pharmacology; D005450:Fluorescamine / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008870:Microtubules / Q000187:drug effects; D010714:Phosphatidylethanolamines / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000502:physiology*
chemical_list: D000199:Actins; D003572:Cytochalasins; D008565:Membrane Proteins; D010714:Phosphatidylethanolamines; C007215:cytochalasin E; D005450:Fluorescamine; D000255:Adenosine Triphosphate; D005033:Ethylmaleimide; D003078:Colchicine; D001542:Benomyl