title: The manganese superoxide dismutase from the penicillin producer Penicillium chrysogenum.
abstract: The antioxidant enzyme superoxide dismutase has been studied in order to define mechanisms for the influence of oxygen on penicillin production. Manganese-containing SOD activity was purified from penicillin-producing cultures of the filamentous fungus Penicillium chrysogenum and reverse genetics was used to identify full-length cDNA and genomic clones. Sequence analysis revealed a 630-bp ORF containing three exons and two introns with fungal consensus splice-site junctions. The deduced amino-acid sequence (210 amino acids; 23.13 kDa) includes conserved residues required for enzymatic activity and metal binding, and shares significant similarity with Mn- and Fe-containing superoxide dismutases. The sod gene is present as a single copy in the genome of different P. chrysogenum strains and its expression level is not correlated with penicillin-G productivity.
authors.affiliation: Laboratorios de Ingeniería Genética and Bioquímica Antibióticos S. A., Avendida de Antibióticos 59 - 61, E-24080 León, Spain.
authors.forename: B
authors.initials: B
authors.lastname: Díez
authors.forename: C
authors.initials: C
authors.lastname: Schleissner
authors.forename: M A
authors.initials: MA
authors.lastname: Moreno
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
authors.forename: A
authors.initials: A
authors.lastname: Collados
authors.forename: J L
authors.initials: JL
authors.lastname: Barredo
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004271:DNA, Fungal / Q000032:analysis; D015870:Gene Expression / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D010406:Penicillins / Q000378:metabolism; D010408:Penicillium chrysogenum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013482:Superoxide Dismutase / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004271:DNA, Fungal; D010406:Penicillins; D013482:Superoxide Dismutase