Rapid and sensitive high-performance liquid chromatographic determination of nicotine and cotinine in nonsmoker human and rat urines.
A simple reversed-phase high-performance liquid chromatographic method with paired-ion and UV detection has been developed for the rapid quantification of urinary nicotine and cotinine. A one-step solid-liquid extraction on Extrelut was used. Separation from endogenous substances was achieved with a decreasing flow-rate. With 20 ml of urine for extraction, the limit of quantification was 0.5 ng/ml for cotinine and 5 ng/ml for nicotine; linearity was obtained from 50 to 5000 ng/ml. The intra- and inter-day coefficients of variation were less than 9% for cotinine and 30% for nicotine. Average recoveries for cotinine were 92-100% and 47-86% for nicotine. The present method was applied to the urine analysis of smokers, nonsmoker children, and experimental animals.
Oddoze
C
C
Faculté de Pharmacie, Marseille, France.
Pauli
A M
AM
Pastor
J
J
D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003367:Cotinine / Q000652:urine*; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D008297:Male; D009538:Nicotine / Q000652:urine*; D051381:Rats; D012680:Sensitivity and Specificity; D012907:Smoking; D013997:Time Factors; D014028:Tobacco Smoke Pollution
D014028:Tobacco Smoke Pollution; D009538:Nicotine; D003367:Cotinine