[Co-regulative effect of PKA-RII and PKC-alpha kinase subspecies on expression of c-myc and c-H-ras in human gastric cancer cells (MGC 80-3)].
Based on preceding experiment, we further studied the co-regulative effects of PKA-R II and PKC-alpha on expression of oncogenes in human gastric cell line MGC 80-3. The c-myc and c-H-ras expression were suppressed in MGC 80-3 cells during HMBA-induced differentiation. At the same time, PKA-R II showed nuclear translocation from cytoplasm, whereas the expression of PKC-alpha shifted from nucleus to cytoplasm. PKA inhibitor (Sigma) was added to block cAMP-PKA pathway when cell differentiation were induced by HMBA. The PKA-R II was still located in cytoplasm but expression of PKC-alpha translocated again into nucleus. Meanwhile, the c-myc and c-H-ras again expressed. This suggested that the changing regulation of oncogene expression were closely related to signalling from nuclear translocation of kinase subspecies. It thus shows the co-regulation effects of two signal system on oncogenes expression.
Wang
D S
DS
School of Oncology, Beijing Medical Unviersity, Beijing Institute for Cancer Research, Cell Biology Dept.
Liang
Y Y
YY
Peng
J
J
D002352:Carrier Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D005786:Gene Expression Regulation; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes / Q000378:metabolism*; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D011494:Protein Kinases / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014407:Tumor Cells, Cultured
D002352:Carrier Proteins; D004791:Enzyme Inhibitors; D047908:Intracellular Signaling Peptides and Proteins; D007527:Isoenzymes; C015482:protein kinase modulator; D000242:Cyclic AMP; D011494:Protein Kinases; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha