title: Transcription activation or repression by phage psi 29 protein p4 depends on the strength of the RNA polymerase-promoter interactions.
abstract: Phage psi 29 protein p4 activates the late A3 promoter and represses the early A2c promoter, in both cases by binding upstream from RNA polymerase (RNAP) and interacting with the C-terminal domain of the RNAP alpha subunit. To investigate how this interaction leads to activation at PA3 and to repression at PA2c, mutant promoters were constructed. We show that the position of protein p4 relative to that of RNAP, which is different at each promoter, does not dictate the outcome of the interaction. Rather, in the absence of a-35 consensus box for sigma A-RNAP activation was observed, while in its presence repression occurred. The results support the view that stabilization of RNAP at the promoter over a threshold level leads to repression.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Monsalve
authors.forename: B
authors.initials: B
authors.lastname: Calles
authors.forename: M
authors.initials: M
authors.lastname: Mencía
authors.forename: M
authors.initials: M
authors.lastname: Salas
authors.forename: F
authors.initials: F
authors.lastname: Rojo
mesh_terms: D017103:Bacillus Phages / Q000201:enzymology / Q000235:genetics*; D018983:DNA Footprinting; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D016297:Mutagenesis, Site-Directed / Q000502:physiology; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding / Q000235:genetics; D012367:RNA, Viral / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D012097:Repressor Proteins; D014157:Transcription Factors; D014764:Viral Proteins; C053987:p4 protein, Bacteriophage phi 29; D012321:DNA-Directed RNA Polymerases