title: Simple spectrophotometric analysis of passive and active ear cutaneous anaphylaxis in the mouse.
abstract: Homologous passive cutaneous anaphylaxis (PCA) and active cutaneous anaphylaxis (ACA) to ovalbumin and DNP-hapten were studied in the ears of female BALB/c mice by means of assessing Evans blue dye leakage. For the quantitative evaluation of PCA and ACA, a hand-held spectrophotometer and the conventional colorimetric method were used to detect the amount of extravasated dye. The value of deltaE*ab (a numerical expression of color) obtained with the hand-held spectrophotometer and the amount of extravasated dye showed a good correlation. In the mouse ear, the sensitivity of PCA reaction was comparable to that of PCA in the rat, and deltaE*ab in the PCA and ACA reactions correlated well with the dilutions of sera and of the antigen, respectively. Thus, using a hand-held spectrophotometer is a simple, quantitative and sensitive method for ascertaining the extent of immediate-type hypersensitivity in the mouse.
authors.affiliation: National Institute of Health Sciences, Tokyo, Japan. rteshima@nihs.go.jp
authors.forename: R
authors.initials: R
authors.lastname: Teshima
authors.forename: H
authors.initials: H
authors.lastname: Akiyama
authors.forename: R
authors.initials: R
authors.lastname: Akasaka
authors.forename: Y
authors.initials: Y
authors.lastname: Goda
authors.forename: M
authors.initials: M
authors.lastname: Toyoda
authors.forename: J
authors.initials: J
authors.lastname: Sawada
mesh_terms: D000707:Anaphylaxis; D000818:Animals; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D010323:Passive Cutaneous Anaphylaxis; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D010047:Ovalbumin