title: The identification of myogenic cells in skeletal muscle, with emphasis on the use of tritiated thymidine autoradiography and desmin antibodies.
abstract: The identification of myogenic precursor cells (mpc) is a key factor in determining the early events in the myogenesis and regeneration of skeletal muscle. Although satellite cells have long been established as the providers of myoblastic cells, very little is really known (apart from their anatomical location in relation to muscle fibres and their ability to migrate) about the precise role of satellite cells in myogenesis. Numerous techniques for labelling mpc have been devised, but none of these has proven to be completely reliable in firmly establishing the origin of myogenic cells. The use of tritiated thymidine to label DNA in proliferating mpc (which are not specifically distinguishable at the time) and the subsequent location of their labelled progeny in myotube nuclei has revealed a great deal of data on the timing of myogenesis, but not about the nature of mpc themselves. DNA synthesis can also be detected by antibodies to the thymidine analogue, bromodeoxyuridine, and also by antibody staining for proliferating nuclear cell antigen. Like tritiated thymidine, these other markers are not specific for muscle but are general markers for DNA synthesis. In situ hybridisation of various muscle-specific genetic markers and their products has been informative, as has immunolabelling of myogenin, MyoD1 and desmin. Desmin labelling has been particularly instructive in identifying mpc because it is one of the first muscle-specific proteins to be produced in mpc. This review covers some of the techniques mentioned above and their usefulness in determining the early events in myogenesis.
authors.affiliation: Department of Anatomy and Human Biology, The University of Western Australia, Nedlands, Australia.
authors.forename: M J
authors.initials: MJ
authors.lastname: Lawson-Smith
authors.forename: J K
authors.initials: JK
authors.lastname: McGeachie
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001345:Autoradiography; D015415:Biomarkers / Q000032:analysis; D002455:Cell Division; D004247:DNA / Q000032:analysis*; D003893:Desmin / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D012038:Regeneration; D013234:Stem Cells / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; D003893:Desmin; D004247:DNA