[Dissociation and culture of primary auditory neurons in postnatal chicken].
In the present study, a primary culture of auditory neurons in 10-16-day old chickens was carried out. We observed the morphologic features of auditory neurons at different culturing stages by using phased-contrast and scanning electron microscope, and particularly described their growth and developmental process. The results indicated that a steady and reliable culturing method established here would contribute to the biological research of inner ear in vitro.
Sun
J
J
Department of Otolaryngology, Union Hospital, Tongji Medical University, Wuhan.
Wang
J
J
Li
G
G
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002645:Chickens; D005260:Female; D008297:Male; D009474:Neurons / Q000166:cytology / Q000648:ultrastructure; D000159:Vestibulocochlear Nerve / Q000166:cytology* / Q000648:ultrastructure