title: Quantification of human immunodeficiency virus type 1 RNA levels in plasma by using small-volume-format branched-DNA assays.
abstract: We have developed small-volume (50 or 250 microl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.
authors.affiliation: Chiron Diagnostics, Emeryville, California 94608-2916, USA. tory_yeghiazarian@cc.chiron.com
authors.forename: T
authors.initials: T
authors.lastname: Yeghiazarian
authors.forename: Y
authors.initials: Y
authors.lastname: Zhao
authors.forename: S E
authors.initials: SE
authors.lastname: Read
authors.forename: W
authors.initials: W
authors.lastname: Kabat
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: S J
authors.initials: SJ
authors.lastname: Hamren
authors.forename: P J
authors.initials: PJ
authors.lastname: Sheridan
authors.forename: J C
authors.initials: JC
authors.lastname: Wilber
authors.forename: D N
authors.initials: DN
authors.lastname: Chernoff
authors.forename: R
authors.initials: R
authors.lastname: Yogev
mesh_terms: D000293:Adolescent; D019380:Anti-HIV Agents / Q000627:therapeutic use; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral; D018450:Disease Progression; D015658:HIV Infections / Q000188:drug therapy / Q000821:virology*; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D012367:RNA, Viral / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D019380:Anti-HIV Agents; D004279:DNA, Viral; D012367:RNA, Viral