Increased transcription of glutamate-aspartate transporter (GLAST/GluT-1) mRNA following kainic acid-induced limbic seizure.
Expression of mRNA for glutamate-aspartate transporter (GLAST/GluT-1/EAAT1) was studied in the brain of the rat which presented recurrent limbic seizure following systemic administration of kainic acid (KA) by in situ hybridization and Northern blot analysis. The expression of GLAST mRNA was markedly increased after 12 h and peaked after 48 h in animals which demonstrated limbic seizure. The induction of the mRNA were observed in the small non-neuronal cells in the hippocampus, especially around CA3 region and hilus. In contrast, there was no change in GLAST mRNA levels in KA injected seizure-free animals. These findings suggest that GLAST mRNA is induced by seizure and increased extracellular glutamate levels during seizure may be important for induction of GLAST mRNA.
Nonaka
M
M
Department of Neurosurgery, Osaka University School of Medicine, Japan.
Kohmura
E
E
Yamashita
T
T
Shimada
S
S
Tanaka
K
K
Yoshimine
T
T
Tohyama
M
M
Hayakawa
T
T
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000235:genetics*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D003292:Convulsants / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D007608:Kainic Acid / Q000008:administration & dosage / Q000494:pharmacology / Q000633:toxicity*; D008032:Limbic System / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D003292:Convulsants; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D018698:Glutamic Acid; D007608:Kainic Acid