title: Establishment and characterization of bone marrow stroma-dependent leukemia cell line, HB-1.
abstract: Radiation-induced M361 leukemia bearing mice (M361 mice) show characteristics of acute myelogenous leukemia (AML) with granulocytosis. Granulocyte-macrophage colony forming unit (CFU-GM) increased in the bone marrow and spleen and high activity of colony stimulating factor (CSF) was found in the sera of M361 mice. A cell line (HB-1) was established from the spleen cells of M361 mice. Injection of HB-1 cells induced a similar leukemic response as M361 in syngeneic mice. HB-1 cells did not survive in the suspension culture, but proliferated when cultured on hemopoietic supportive stromal cells (MS-10). HB-1 cells appear to be strictly dependent on the hemopoietic supportive bone marrow stroma, which would provide a useful model for the study of cell-cell interactions between hemopoietic cells and marrow stromal cells.
authors.affiliation: Department of Internal Medicine, Kokura Memorial Hospital, Kitakyushu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sawada
authors.forename: K
authors.initials: K
authors.lastname: Sugimoto
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: Y
authors.initials: Y
authors.lastname: Tsurumaki
authors.forename: G
authors.initials: G
authors.lastname: Breckon
authors.forename: S A
authors.initials: SA
authors.lastname: Lorimore
authors.forename: E G
authors.initials: EG
authors.lastname: Wright
authors.forename: K J
authors.initials: KJ
authors.lastname: Mori
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000473:pathology; D002450:Cell Communication; D002470:Cell Survival; D018920:Coculture Techniques; D006801:Humans; D007942:Leukemia, Experimental / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D051379:Mice; D017154:Stromal Cells / Q000473:pathology*; D014407:Tumor Cells, Cultured