[Effect of combining EGF with KGF on proliferation of human corneal epithelial cells].
OBJECTIVE
To select effective drugs for enhancing corneal epithelial wound healing and treating persistent corneal epithelial defect.


METHODS
Epidermal growth factor (EGF) and keratinocyte growth factor (KGF) were added to cultures of human corneal epithelial cells and DNA synthesis in corneal epithelial cells were measured with [3H]-thymidine incorporation and liquid scintillation techniques. The cells were counted in a hemocytometer to determine the doubling time.


RESULTS
10 ng/ml EGF, 10 ng/ml KGF enhanced cellular proliferation effectively (P < 0.01). EGF in combination with KGF, the enhancement was more effective (P < 0.05) and reduced the doubling time.


CONCLUSIONS
Our results suggest that exogenous EGF with KGF can stimulate proliferation of human corneal epithelial cells and it may be of potential use clinically for treatment of persistent corneal epithelial
Gong
X
X
Zhongshan Ophthalmic Center, Sum Yat-sen University of Medical Sciences, Guangzhou, China.
Zhong
X
X
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D019573:Epithelium, Corneal / Q000166:cytology / Q000187:drug effects*; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans
C496453:FGF7 protein, human; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor; D004247:DNA