title: Determination of ascorbic acid in nanolitre samples by means of capillary batch injection analysis.
abstract: Capillary batch injection analysis (CBIA) is applied to the determination of ascorbic acid in nanolitre samples at neutral pH. It is demonstrated that pulsed amperometric detection is superior to constant-potential amperometric detection with respect to signal stability. The calibration plot is linear within the studied concentration range between 0.5 mM and 5 mM and the reproducibility is characterized by a relative standard deviation of 0.80% for 10 successive injections of 188 nL of 3.5 mM ascorbic acid. The attainable sample throughput is up to 500 per hour.
authors.affiliation: Forschungszentrum Karlsruhe, Institut für Instrumentelle Analytik, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Backofen
authors.forename: F M
authors.initials: FM
authors.lastname: Matysik
authors.forename: W
authors.initials: W
authors.lastname: Hoffmann
authors.forename: H J
authors.initials: HJ
authors.lastname: Ache
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D004566:Electrodes; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry; D012680:Sensitivity and Specificity
chemical_list: D001205:Ascorbic Acid