title: Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression.
abstract: This study describes the cloning of two cDNAs encoding three serine-protease-inhibiting peptides, SGPI I, II and III, which were recently identified from ovarian extracts of the desert locust, Schistocerca gregaria. The first cDNA codes for the precursor polypeptides of SGPI I and SGPI II; the second encodes only a single inhibitor, SGPI III. Northern-blot analysis revealed an approximate length of 0.8 kb for SGPI-I/II mRNA and 0.6 kb for SGPI-III mRNA. The transcripts are present in several locust tissues, but they could not be detected in the midgut. The gene for SGPI-I/II is abundantly transcribed during all larval and adult stages, whereas SGPI-III mRNA is mainly present in adults. Northern-blot hybridization also revealed important changes in the SGPI-mRNA content during the molting cycle and during the adult reproductive cycle. Moreover, a differential hormonal control was observed in adult females which had been treated with precocene, juvenile hormone or ecdysone.
authors.affiliation: Laboratory for Developmental Physiology and Molecular Biology, Leuven, Belgium. jozef.vandenbroeck@bio.kuleuven.ac.be
authors.forename: J
authors.initials: J
authors.lastname: Vanden Broeck
authors.forename: S J
authors.initials: SJ
authors.lastname: Chiou
authors.forename: L
authors.initials: L
authors.lastname: Schoofs
authors.forename: A
authors.initials: A
authors.lastname: Hamdaoui
authors.forename: F
authors.initials: F
authors.lastname: Vandenbussche
authors.forename: G
authors.initials: G
authors.lastname: Simonet
authors.forename: S
authors.initials: S
authors.lastname: Wataleb
authors.forename: A
authors.initials: A
authors.lastname: De Loof
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001578:Benzopyrans / Q000494:pharmacology; D003001:Cloning, Molecular; D004441:Ecdysterone / Q000494:pharmacology; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006110:Grasshoppers / Q000502:physiology*; D019476:Insect Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010053:Ovary / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D011498:Protein Precursors / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry*; D012717:Sesquiterpenes / Q000494:pharmacology; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: D001578:Benzopyrans; D004441:Ecdysterone; D019476:Insect Proteins; D010455:Peptides; D011498:Protein Precursors; D012333:RNA, Messenger; D015842:Serine Proteinase Inhibitors; D012717:Sesquiterpenes; C113210:LOC126284306 protein, Schistocerca gregaria; C113212:SGPI III protein, Schistocerca gregaria; C019539:precocene I; C036585:juvenile hormone III