title: Analysis of TNFB and TNFA NcoI RFLP in colorectal cancer.
abstract: We examined the relationship between the NcoI RFLP of tumor necrosis factor beta (TNFB) and alpha (TNFA) genes and the risk of colorectal cancer. The first intron of TNFB and the -308 promoter region of TNFA NcoI RFLP were ascertained from peripheral leucocytes of 136 colorectal cancer patients and 325 healthy controls. The TNFB*1/TNFB*1 homozygote was significantly predominant in patients with colorectal cancer (18.4%) compared to control subjects (9.8%) (p < 0.01), whereas there was inverse association in TNFB*2/TNFB*2. However, the association between colorectal cancer and TNFA genotypes was not found which indicates that these alleles did not appear to be a susceptibility factor for colorectal cancer. TNFB polymorphism was not associated with a clinicopathological parameter of colorectal cancer. However, in regard to the degree of recurrence during the postoperative survival period, TNFB*1/TNFB*1 (12.5%) and TNFB*1/TNFB*2 (24%) were higher than TNFB*2/TNFB*2 (8.3%). Based on these results, it indicates that TNFB*1/TNFB*1 genotypes show an increased risk for colorectal cancer, and that the TNFB*1 allele (R.R. = 1.41) mediates some role in the initial step of tumorigenesis or activation of dormant tumor cells, whereas TNFB*2 allele mediates some functions associated with cytotoxicity of tumor cells.
authors.affiliation: Department of Biology, Sungshin Women's University, Seoul, Korea. kspark@cc.sungshin.ac.kr
authors.forename: K S
authors.initials: KS
authors.lastname: Park
authors.forename: J W
authors.initials: JW
authors.lastname: Mok
authors.forename: S A
authors.initials: SA
authors.lastname: Rho
authors.forename: J C
authors.initials: JC
authors.lastname: Kim
mesh_terms: D000483:Alleles; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D005260:Female; D005787:Gene Frequency; D005796:Genes / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D004273:DNA, Neoplasm; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; C052380:endodeoxyribonuclease NcoI; D015252:Deoxyribonucleases, Type II Site-Specific