The potential of various HIV-1 mutants to inhibit the replication of wild-type virus.
We have previously demonstrated that many of gag mutants of human immunodeficiency virus type 1 (HIV-1) inhibited the replication of wild-type (wt) HIV-1. In this study, various HIV-1 mutants were systematically analyzed with respect to their ability to suppress the replication of wt HIV-1. Sixteen mutants of all eight HIV-1 genes other than gag were evaluated for their inhibitory effects. Only an env mutant designated NL-Hi efficiently interfered with the replication of wt HIV-1 in a single round of infection. The NL-Hi did not affect the late replication processes of wt virus, including transcription, translation, and assembly/release. Virions produced in the presence of the mutant Env were defective for the viral entry process in the early phase of HIV-1 replication cycle.
Inubushi
R
R
Department of Virology, The University of Tokushima School of Medicine, Tokushima, 770-8503, Japan.
Shimano
R
R
Oshima
Y
Y
Adachi
A
A
D002460:Cell Line; D015870:Gene Expression; D005814:Genes, Viral; D015752:Genes, env; D015750:Genes, gag; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D009154:Mutation; D010641:Phenotype; D014762:Viral Interference / Q000235:genetics; D014779:Virus Replication / Q000235:genetics*