title: Development of a highly sensitive high-performance liquid chromatographic method for measuring an anticancer drug, UCN-01, in human plasma or urine.
abstract: We have established a highly sensitive high-performance liquid chromatographic method for the determination of an anticancer drug, UCN-01, in human plasma or urine. Using a fluorescence detector set at an excitation wavelength of 310 nm and emission monitored at 410 nm, there was a good linearity for UCN-01 in human plasma (r=0.999) or urine (r=0.999) at concentrations ranging from 0.2 to 100 ng/ml or 1 to 400 ng/ml, respectively. For intra-day assay, in plasma samples, the precision and accuracy were 1.8% to 5.6% and -10.0% to 5.2%, respectively. For inter-day assay, the precision and accuracy were 2.0% to 18.2% and 2.4% to 10.0%, respectively. In urine samples, the intra- and inter-day precision and accuracy were within 3.9% and +/-2.7%, respectively. The lower limit of quantification (LLOQ) was set at 0.2 ng/ml in plasma and 1 ng/ml in urine. UCN-01 in plasma samples was stable up to two weeks at -80 degrees C and also up to four weeks in urine samples. This method could be very useful for studying the human pharmacokinetics of UCN-01.
authors.affiliation: Drug Development Research Laboratories, Pharmaceutical Research Institute, Kyowa Hakko Kogyo Co., Ltd., Shizuoka-ken, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Kurata
authors.forename: T
authors.initials: T
authors.lastname: Kuramitsu
authors.forename: H
authors.initials: H
authors.lastname: Tanii
authors.forename: E
authors.initials: E
authors.lastname: Fuse
authors.forename: T
authors.initials: T
authors.lastname: Kuwabara
authors.forename: H
authors.initials: H
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Kobayashi
mesh_terms: D000470:Alkaloids / Q000032:analysis* / Q000097:blood / Q000652:urine; D000970:Antineoplastic Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006801:Humans; D012680:Sensitivity and Specificity; D019311:Staurosporine / Q000031:analogs & derivatives
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; C054852:7-hydroxystaurosporine; D019311:Staurosporine