title: The gene for component-II of botulinum C2 toxin.
abstract: The gene encoding component-II of the Clostridium botulinum C2 toxin was cloned from the chromosomal DNA of C. botulinum type C strain (C)-203U28, and the nucleotide sequence was determined. The gene (bc2h) encodes a protein with 721 amino acid residues and is located at 247 bp downstream of the gene for component-I. The N-terminal 16 amino acids were identical to those obtained by analysis of the purified component-II toxin. The ORF for bc2h had only 39% homology at the amino acid level with the C.perfringens iota-Ib protein and an ATP/GTP binding site which is present in the iota-Ib protein is missing from the protein encoded by bc2h. Both genes had a homologous region that predicts a transmembrane segment.
authors.affiliation: Department of Microbiology, Sapporo Medical University School of Medicine, Hokkaido, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: I
authors.initials: I
authors.lastname: Ohishi
authors.forename: E
authors.initials: E
authors.lastname: Isogai
authors.forename: H
authors.initials: H
authors.lastname: Isogai
authors.forename: N
authors.initials: N
authors.lastname: Fujii
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001905:Botulinum Toxins / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003014:Clostridium botulinum / Q000737:chemistry / Q000235:genetics*; D003016:Clostridium perfringens / Q000737:chemistry; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D001905:Botulinum Toxins