Features of Tri101, the trichothecene 3-O-acetyltransferase gene, related to the self-defense mechanism in Fusarium graminearum.
A structural gene of Tri101, which encodes trichothecene 3-O-acetyltransferase, was isolated as a 3-kb XhoI-XbaI fragment from the trichothecene producer Fusarium graminearum strain F15. The gene contained no introns, and the coding region was 0.7-kb downstream of a putative UTP-ammonia ligase gene which obviously is not related to the biosynthesis of trichothecenes. Tri101 was expressed when T-2 toxin was added, but this induction was not dependent on the expression level of Tri6, a transcription activator gene in the trichothecene biosynthetic and regulatory gene cluster.
Kimura
M
M
Microbial Toxicology Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. mkimura@postman.riken.go.jp
Shingu
Y
Y
Yoneyama
K
K
Yamaguchi
I
I
D000123:Acetyltransferases / Q000235:genetics* / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D001483:Base Sequence; D005670:Fusarium / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
D000123:Acetyltransferases; C110205:trichothecene 3-O-acetyltransferase