title: Effects of pluronic block copolymers on drug absorption in Caco-2 cell monolayers.
abstract: OBJECTIVE
  The present work characterizes the effects of Pluronic copolymers on the transport of a P-gp-dependent probe, rhodamine 123 (R123) in Caco-2 cell monolayers.
  
  
  METHODS
  The accumulation and efflux studies were performed on the confluent Caco-2 monolayers using fluorescent probes with and without Pluronic copolymers.
  
  
  RESULTS
  At concentrations below the critical micelle concentration single chains ("unimers") of Pluronic P85 enhanced the accumulation and inhibited the efflux of R123 in Caco-2 monolayers. The transport of the P-gp-independent probe, rhodamine 110 was not altered under these conditions. In contrast the micelles increased R123 accumulation to a much lower extent when compared to the unimers and enhanced R123 efflux in Caco-2 monolayers.
  
  
  CONCLUSIONS
  Pluronic P85 unimers increase accumulation of a P-gp-dependent drug in Caco-2 monolayers through inhibition of the P-gp efflux system. The mechanism of the micelle effect is not known, however, it is very similar to the micelle effects in BBMEC. This has been previously shown to involve vesicular transport of the micelle-incorporated drug. The study suggests that Pluronic copolymers can be useful in increasing oral absorption of select drugs.
authors.affiliation: Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha 68198, USA.
authors.forename: E V
authors.initials: EV
authors.lastname: Batrakova
authors.forename: H Y
authors.initials: HY
authors.lastname: Han
authors.lastname: Alakhov VYu
authors.forename: D W
authors.initials: DW
authors.lastname: Miller
authors.forename: A V
authors.initials: AV
authors.lastname: Kabanov
mesh_terms: D018938:Caco-2 Cells; D003902:Detergents; D006801:Humans; D008823:Micelles; D008856:Microscopy, Fluorescence; D010599:Pharmacokinetics; D011060:Poloxalene / Q000494:pharmacology*
chemical_list: D003902:Detergents; D008823:Micelles; D011060:Poloxalene