title: Characterization of mastoparan-induced histamine release from RBL-2H3 cells.
abstract: Mastoparan (5-30 microM), a tetradecapeptide isolated from wasp venom, caused histamine release from RBL-2H3 cells in a concentration- and time-dependent manner. Mastoparan-induced histamine release remained after removing the extracellular Ca2+, whereas the antigen-induced one disappeared. Pertussis toxin did not inhibit mastoparan-induced histamine release from the cells, and mastoparan did not stimulate phosphoinositide hydrolysis. In agreement with the results, RBL-2H3 cells had a small amount of ADP-ribosylation substrates for pertussis toxin. Neomycin (1-5 mM) suppressed mastoparan-induced histamine release and phospholipase D activation. However, butanol slightly inhibited mastoparan-induced histamine release. Moreover, 2,3-diphosphoglycerate inhibited mastoparan-induced phospholipase D activation, but not it's histamine release. On the other hand, mastoparan caused the leakage of lactate dehydrogenase from the cells in a similar concentration range to the histamine release. This leakage was also suppressed by neomycin. These results suggest that mastoparan enhances the membrane permeability, resulting in histamine release in a pertussis toxin-insensitive manner, and that mastoparan-induced phospholipase D activation may not relate to histamine release.
authors.affiliation: Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Mizuno
authors.forename: N
authors.initials: N
authors.lastname: Nakahata
authors.forename: Y
authors.initials: Y
authors.lastname: Ohizumi
mesh_terms: D020001:1-Butanol / Q000494:pharmacology; D019794:2,3-Diphosphoglycerate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D036341:Intercellular Signaling Peptides and Proteins; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015471:Leukemia, Basophilic, Acute; D009355:Neomycin / Q000494:pharmacology; D010455:Peptides; D037342:Pertussis Toxin; D010716:Phosphatidylinositols / Q000378:metabolism; D010739:Phospholipase D / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured / Q000187:drug effects; D010566:Virulence Factors, Bordetella / Q000494:pharmacology; D014862:Wasp Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D010716:Phosphatidylinositols; D010566:Virulence Factors, Bordetella; D014862:Wasp Venoms; D019794:2,3-Diphosphoglycerate; C037500:mastoparan; D020001:1-Butanol; D007770:L-Lactate Dehydrogenase; D037342:Pertussis Toxin; D010739:Phospholipase D; D009355:Neomycin; D002118:Calcium