Variable gene segment-specific N-insertions at the signal joint of T-cell receptor Vbeta-Dbeta recombinations.
The V(D)J recombination of immunoglobulin and a T-cell receptor generates two species of DNA junctions, a coding joint and a signal joint. Non-templated nucleotides (N-nucleotides) are inserted in these DNA junctions. We analyzed the N-insertion at signal joints generated by the Vbeta-Dbeta recombinations. N-insertions were detected at signal joints of Vbeta2, Vbeta3, Vbeta10, Vbeta18 and Vbeta14 but not in Vbeta8 and Vbeta7. These data show that the N-insertion at signal joints is dependent on the Vbeta locus used for the recombination. We suggest that the regional chromosomal configuration may differ in recombinase accessibility.
Kanari
Y
Y
Department of Biophysics, Graduate School of Science, Kyoto University, Japan.
Nakagawa
R
R
Arakawa
H
H
Yamagishi
H
H
D000818:Animals; D001483:Base Sequence; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D011995:Recombination, Genetic
D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta