Allelic loss analysis of gamma-ray-induced mouse thymic lymphomas: two candidate tumor suppressor gene loci on chromosomes 12 and 16.
A total of 429 gamma-ray-induced thymic lymphomas were obtained from F1 and backcross mice between BALB/c and MSM strains, about a half of which carried a p53-deficient allele. A genome-wide allelic loss analysis has revealed two loci exhibiting frequent allelic losses but no allelic preference, one is localized within a 2.9 cM region between D12Mit53 and D12Mit279 loci on chromosome 12, and the other is near the D16Mit122/D16Mit162 loci on chromosome 16. The frequency of allelic loss in the D12Mit279 region is 62% and does not differ in tumors between the presence and absence of the p53-deficient allele. In contrast, the loss frequency of D16Mit122 is raised by the existence of p53-deficient allele: 62% for p63(-/+) and 13% for p53(+/+), suggesting co-operative function of the two losses. The D12Mit279 and D16Mit122 regions probably harbor different types of tumor suppressor gene that play key roles in lymphoma development.
Matsumoto
Y
Y
Department of Biochemistry, Niigata University School of Medicine, Asahimachi, Japan.
Kosugi
S
S
Shinbo
T
T
Chou
D
D
Ohashi
M
M
Wakabayashi
Y
Y
Sakai
K
K
Okumoto
M
M
Mori
N
N
Aizawa
S
S
Niwa
O
O
Kominami
R
R
D000818:Animals; D002874:Chromosome Mapping; D005260:Female; D005720:Gamma Rays; D016147:Genes, Tumor Suppressor; D005838:Genotype; D019656:Loss of Heterozygosity; D008223:Lymphoma / Q000235:genetics*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013953:Thymus Neoplasms / Q000235:genetics*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D016159:Tumor Suppressor Protein p53