The nuclear concentration of kin17, a mouse protein that binds to curved DNA, increases during cell proliferation and after UV irradiation.
UV-irradiation induces, in mammalian cells, the expression of a set of genes known as the 'UV-response', which may be reminiscent of the bacterial response, called SOS system. The multifunctional protein RecA controls the expression of the SOS genes. We report the expression profile of a mouse gene conserved among mammals, called Kin17, that codes a DNA-binding protein of undetermined biochemical activity and which shares epitopes with the bacterial RecA protein. We demonstrate that the level of Kin17 RNA was 5-fold higher in mid-S phase of serum-stimulated BALB/c 3T3 fibroblasts than in quiescent cells. Cells in S-phase displayed a high level of kin17 protein with a marked nuclear localisation. The maximal level of Kin17 RNA was observed 18 h after serum stimulation, indicating that Kin17 gene is a new member of the late growth-related genes. The accumulation of kin17 protein during cell proliferation follows the increase in Kin17 RNA and correlates with DNA synthesis, which suggests a possible role of kin17 protein in a transaction related to DNA-replication. In quiescent fibroblasts, a 3-fold increase in Kin17 RNA was seen 13 h after UV irradiation. In parallel, kin17 protein accumulated in the nucleus, which suggests that it might be required after the stress produced by UV irradiation.
Kannouche
P
P
Département de Radiobiologie et de Radiopathologie, Direction des Sciences du Vivant, Centre d'Etudes de Fontenay-aux-Roses, France.
Pinon-Lataillade
G
G
Tissier
A
A
Chevalier-Lagente
O
O
Sarasin
A
A
Mezzina
M
M
Angulo
J F
JF
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D016895:Culture Media, Serum-Free; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000528:radiation effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins; D011485:Protein Binding; D012313:RNA / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014466:Ultraviolet Rays
D016895:Culture Media, Serum-Free; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C071214:Kin protein, mouse; D012313:RNA; D004247:DNA; D013755:Tetradecanoylphorbol Acetate