beta-Glucosylation of chitooligomers by galactosyltransferase.
Galactosyltransferase from bovine milk was found to be able to utilise UDP-Glc to transfer Glc onto GlcNAc and chitooligomers[-beta-GlcNAc-(1-->4)-]n, n = 2-4. beta-Glucosylated products were used in binding studies with NKR-P1A protein cloned from rat natural killer cells.
Kren
V
V
Institute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Prague, Czech Republic. kren@biomed.cas.cz
Dvoráková
J
J
Gambert
U
U
Sedmera
P
P
Havlícek
V
V
Thiem
J
J
Bezouska
K
K
D000117:Acetylglucosamine / Q000737:chemistry*; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D001667:Binding, Competitive; D002240:Carbohydrate Sequence; D002417:Cattle; D005700:Galactosyltransferases / Q000378:metabolism*; D005960:Glucosides / Q000138:chemical synthesis*; D007694:Killer Cells, Natural / Q000737:chemistry; D037181:Lectins, C-Type; D009682:Magnetic Resonance Spectroscopy; D008892:Milk / Q000201:enzymology; D008969:Molecular Sequence Data; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides / Q000138:chemical synthesis; D011485:Protein Binding / Q000502:physiology; D051381:Rats; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D000954:Antigens, Surface; D005960:Glucosides; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009844:Oligosaccharides; D005700:Galactosyltransferases; D014532:Uridine Diphosphate Glucose; D000117:Acetylglucosamine