Cypermethrin increases apo A-1 and apo B mRNA but not hyperlipidemia in rats.
The hepatotoxic effect of cypermethrin and the expression of hepatic genes at the mRNA level, as molecular markers of liver damage, were evaluated in rats following exposure to cypermethrin. The expression of hepatic genes was compared with conventional liver functional tests, and correlations were made by studying the liver at the ultrastructural level. Cypermethrin treated rats presented a significant decrease, of 79% and 22%, on the expression of albumin and apo E genes at 5 days, respectively. The levels of apo A-1 and apo B mRNA were increased up to four- and fivefold, respectively. This increase did not correlate with the serum values of HDL and VLDL lipoprotein particles. Intracytoplasmic lipid droplets were observed after the first 2 days following cypermethrin administration, suggesting that apo A-1 and B mRNA were translated but not secreted. There were significant correlations between the low values of the albumin gene expression, the decrease in the HDL concentrations, and the ultrastructural alterations, respectively. These alterations were mainly a large amount and increased size of mitochondria in the animals exposed to cypermethrin. It is concluded that under the experimental conditions used, cypermethrin may alter the metabolism of lipids and proteins in rat liver.
Aldana
L
L
Universidad Autónoma de Querétaro, Mexico.
González de Mejía
E
E
Craigmill
A
A
Tsutsumi
V
V
Armendariz-Borunda
J
J
Panduro
A
A
Rincón
A R
AR
D000418:Albumins / Q000032:analysis; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics / Q000378:metabolism*; D001055:Apolipoproteins B / Q000235:genetics / Q000378:metabolism*; D006949:Hyperlipidemias / Q000139:chemically induced*; D007306:Insecticides / Q000633:toxicity*; D008075:Lipoproteins, HDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D014176:Protein Biosynthesis / Q000187:drug effects; D011722:Pyrethrins / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D000418:Albumins; D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D007306:Insecticides; D008075:Lipoproteins, HDL; D008079:Lipoproteins, VLDL; D011722:Pyrethrins; D012333:RNA, Messenger; C017160:cypermethrin