Structural organisations of hemoglobin and myoglobin influence their binding behaviour with phenothiazines.
Binding modalities of chlorpromazine and trifluoperazine, two widely used antipsychotic phenothiazine drugs with hemoglobin and myoglobin have been studied to understand how the quaternary, tertiary and secondary structural organisations of the proteins regulate the binding process. NaCl-induced alteration in the quaternary structure of hemoglobin influences its binding modality with phenothiazines. Minor alterations in the tertiary structure of thermally denatured myoglobin (denaturation temperature ranging between 30-70 degrees C) do not affect its affinity and the modality of binding with the drugs, but alterations in the secondary structure of the protein denatured at temperatures between 70-80 degrees C influence its binding.
Bhattacharyya
J
J
Department of Biophysics, Molecular Biology and Genetics, University College of Science, University of Calcutta, India.
Bhattacharyya
M
M
Chakraborti
A S
AS
Chaudhuri
U
U
Poddar
R K
RK
D000328:Adult; D014150:Antipsychotic Agents / Q000737:chemistry / Q000378:metabolism*; D002746:Chlorpromazine / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D006454:Hemoglobins / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D046911:Macromolecular Substances; D008297:Male; D009211:Myoglobin / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013816:Thermodynamics; D014268:Trifluoperazine / Q000737:chemistry / Q000378:metabolism
D014150:Antipsychotic Agents; D006454:Hemoglobins; D046911:Macromolecular Substances; D009211:Myoglobin; D014268:Trifluoperazine; D002746:Chlorpromazine