title: Evaluation of taurine metabolism in cats by dual stable isotope analysis.
abstract: Taurine kinetics in cats was investigated using a bolus dose of [15N]- and [1,2-13C2]taurine. The comparison of [15N]- and [13C2]taurine kinetics permitted an evaluation of the extent of taurine transamination. A methodology which involves N-pentafluorobenzoyl di-n-butylamine derivatization of taurine and GC/MS measurements of the 15N- and 13C-enrichments in cat urine was developed. Accuracy of the measurements was determined using pure standard compounds and the results showed that [13C2]taurine does not interfere with [15N]taurine. In cats, no differences were observed between both tracers. Therefore, we conclude that taurine reversible transamination does not occur at a significant level in cats.
authors.affiliation: Nestlé Research Centre (Friskies Research), Nestec Ltd., Lausanne, Switzerland. laurent.fay@chlsnr.nestrd.ch
authors.forename: L B
authors.initials: LB
authors.lastname: Fay
authors.forename: S
authors.initials: S
authors.lastname: Métairon
authors.forename: F
authors.initials: F
authors.lastname: Montigon
authors.forename: O
authors.initials: O
authors.lastname: Ballèvre
mesh_terms: D000818:Animals; D002247:Carbon Isotopes; D002415:Cats; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D015203:Reproducibility of Results; D013654:Taurine / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine
chemical_list: D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D013654:Taurine