Respiratory burst assay of head kidney macrophages of ayu, Plecoglossus altivelis, stimulated with Glugea plecoglossi (Protozoa: Microspora) spores.
The respiratory burst assay was conducted using the nitroblue tetrazolium reduction method and horseradish peroxidase method to investigate the events when ayu head kidney macrophages phagocytize fresh and formalin-killed Glugea plecoglossi spores. The production of O2 against G. plecoglossi spores was negligible compared to zymosan (P < 0.01). Zymosan-induced O2 production was markedly inhibited by adding G. plecoglossi spores simultaneously (P < 0.01). This phenomenon was dose dependent, and killed spores were less inhibitory than fresh spores. The production of H2O2 was drastically increased when G. plecoglossi spores were added (P < 0.01), and most spores were phagocytized. From these results, it is suggested that G. plecoglossi spores modulate the host's phagocytic response to establish infection.
Kim
J H
JH
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.
Ogawa
K
K
Wakabayashi
H
H
D000818:Animals; D002452:Cell Count; D005399:Fishes; D005562:Formazans / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007202:Indicators and Reagents; D007668:Kidney / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism; D016814:Microsporidia / Q000276:immunology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010084:Oxidation-Reduction; D010587:Phagocytosis / Q000502:physiology*; D010637:Phenolsulfonphthalein / Q000378:metabolism; D016897:Respiratory Burst; D013170:Spores / Q000276:immunology; D015054:Zymosan / Q000378:metabolism
D005562:Formazans; D007202:Indicators and Reagents; D009580:Nitroblue Tetrazolium; D015054:Zymosan; D006861:Hydrogen Peroxide; D010637:Phenolsulfonphthalein