Formation of adenosine 3'-monophosphate in rat liver mitochondria.
An unknown adenine-related compound (UKC) in rat liver mitochondria was characterized. Based on the sensitivity to periodate oxidation, nuclease P1 digestion, property of fluorescence derivatization, elution behavior on different separation modes of HPLC columns and the mass spectrum of purified UKC, the UKC was identified as adenosine 3'-monophosphate (3'-AMP), an intracellular P-site inhibitor of adenylate cyclase. 3'-AMP may be enzymatically produced from RNA in rat liver mitochondria in temperature- and time-dependent manners. A partial characterization of 3'-AMP forming enzyme is included.
Fujimori
H
H
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Pan-Hou
H
H
D000249:Adenosine Monophosphate / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008930:Mitochondria, Liver / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D010504:Periodic Acid; D051381:Rats; D017208:Rats, Wistar
D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D010504:Periodic Acid; D000249:Adenosine Monophosphate; C009288:metaperiodate