Generation and preliminary characterization of monoclonal antibodies directed to glycerophospholipid:cholesterol acyltransferase (GCAT) native epitopes of Aeromonas salmonicida.
Four monoclonal antibodies (MAbs) directed to native glycerophospholipid:cholesterol acyltransferase (GCAT) epitopes of Aeromonas salmonicida were isolated using an esterase capture assay. The molecular mass of this MAb-defined antigen was estimated to be 26 kDa in SDS-PAGE. Three different epitope specificities of these MAbs were demonstrated. It was shown that all 4 MAbs recognize GCAT in culture filtrates of the strain MT004 excluding the simultaneous trapping of other components. None of the MAbs react with the denatured GCAT in Western blots.
Lachmann
I
I
Institute of Zoology, University of Leipzig, Germany. ilachman@server1.rz.uni-leipzig.de
Wagner
U
U
Hädge
D
D
Drössler
K
K
D000217:Acyltransferases / Q000276:immunology*; D000333:Aeromonas / Q000201:enzymology / Q000276:immunology*; D000818:Animals; D000907:Antibodies, Bacterial / Q000737:chemistry / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001667:Binding, Competitive; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D018604:Epitope Mapping / Q000662:veterinary; D000939:Epitopes / Q000276:immunology; D005393:Fish Diseases / Q000276:immunology; D016905:Gram-Negative Bacterial Infections / Q000276:immunology / Q000662:veterinary; D006825:Hybridomas; D008970:Molecular Weight; D011233:Precipitin Tests / Q000662:veterinary; D012474:Salmon
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000939:Epitopes; D000217:Acyltransferases; C020597:glycerophospholipid-cholesterol acyltransferase