title: Rat dorsal root ganglion neurones express different capsaicin-evoked Ca2+ transients and permeabilities to Mn2+.
abstract: Capsaicin (1 microM) evoked [Ca2+]i transients in two-thirds of a Percoll-gradient enriched population of rat dorsal root ganglion (DRG) neurones. Amongst the capsaicin-sensitive neurones, 68% responded to capsaicin with peak [Ca2+]i transients of 681 +/- 67 nM, whereas the remaining neurones gave peak [Ca2+]i transients of 260 +/- 84 nM. In the presence of Mn2+ in Ca2+-free medium, capsaicin evoked quenching of fura-2 fluorescence, due to Mn2+ influx, in a similar proportion of neurones. Two patterns of capsaicin-sensitive Mn2+ quenching were observed: 72% of neurones showed rapid quenching (t1/2 of 18.3 +/- 4.1 s), whereas the remaining neurones had low quenching rates (t1/2 of 119 +/- 33 s). Distinct capsaicin-sensitive subpopulations of DRG neurones can thus be distinguished on the basis of their peak [Ca2+]i transient amplitudes, which appear to be associated with different rates of Ca2+ influx.
authors.affiliation: Department of Pharmacy, University of Sydney, NSW, Australia.
authors.forename: V N
authors.initials: VN
authors.lastname: Dedov
authors.forename: B D
authors.initials: BD
authors.lastname: Roufogalis
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects*; D002211:Capsaicin / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D005456:Fluorescent Dyes / Q000378:metabolism; D016257:Fura-2 / Q000378:metabolism; D005727:Ganglia, Spinal / Q000187:drug effects / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
chemical_list: D015220:Calcium Channels; D005456:Fluorescent Dyes; D008345:Manganese; D002211:Capsaicin; D002118:Calcium; D016257:Fura-2