Expression of the human muscarinic receptor gene m2 in Dictyostelium discoideum.
We have expressed a functional human muscarinic M2 receptor, under the control of the homologous discoidin I gamma promoter, in the cellular slime mold Dictyostelium discoideum. The use of a contact site A leader peptide ensured insertion of the newly synthesized receptor protein into the plasma membrane. Due to the characteristics of the discoidin I gamma promoter, the M2 receptor is expressed during late growth and early development. The heterologously expressed M2 receptors show binding characteristics similar to authentic receptors. Membranes as well as whole cells can be used in ligand binding assays.
Voith
G
G
Institut für Pharmazeutische Biologie, Johann Wolfgang Goethe Universität, Frankfurt, Germany.
Dingermann
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001570:Benzodiazepinones / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D004023:Dictyostelium / Q000235:genetics*; D000070821:Discoidins; D005656:Fungal Proteins / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D037102:Lectins; D008969:Molecular Sequence Data; D018727:Muscarinic Antagonists / Q000378:metabolism; D010276:Parasympatholytics / Q000378:metabolism; D010880:Piperidines / Q000378:metabolism; D011401:Promoter Regions, Genetic; D021382:Protein Sorting Signals / Q000235:genetics; D015800:Protozoan Proteins; D011976:Receptors, Muscarinic / Q000737:chemistry / Q000235:genetics*
D001570:Benzodiazepinones; D000070821:Discoidins; D005656:Fungal Proteins; D037102:Lectins; D018727:Muscarinic Antagonists; D010276:Parasympatholytics; D010880:Piperidines; D021382:Protein Sorting Signals; D015800:Protozoan Proteins; D011976:Receptors, Muscarinic; C058297:4-fluorohexahydrosiladifenidol; C069054:AQ-RA 741