title: Dictyostelium discoideum cells shed vesicles with associated DNA and vital stain Hoechst 33342.
abstract: Dictyostelium discoideum cells are highly resistant to xenobiotics. We previously observed that these primitive eukaryotic cells contain a 170-kDa P-glycoprotein, mediating multidrug resistance in mammalian cells, but nonfunctional in Dictyostelium cells. We show here that D. discoideum cells vitally stained with the DNA-specific dye, Hoechst 33342, release fluorescent material in their culture medium. Electron microscopy and lipid analysis demonstrate the vesicular nature of this material. Moreover, nucleic acids associate with these extracellular vesicles independently of Hoechst vital staining. The main vesicular DNA component exhibits a size > 21 kb. Shedding of microvesicles during cell growth is not concomitant with programmed cell death. We propose that these extracellular vesicles are involved in a new cellular resistance mechanism against xenobiotics. Furthermore, since the association of DNA with vesicles occurs in physiological growth conditions and independently of vital staining, the new shedding process might be involved in a more general intercellular mechanism.
authors.affiliation: Laboratoire de Physicochimie Biomoléculaire et Cellulaire, CNRS URA 2056, Université Pierre et Marie Curie, Paris, France. tati@lpbc.jussieu.fr
authors.forename: I
authors.initials: I
authors.lastname: Tatischeff
authors.forename: M
authors.initials: M
authors.lastname: Bomsel
authors.forename: C
authors.initials: C
authors.lastname: de Paillerets
authors.forename: H
authors.initials: H
authors.lastname: Durand
authors.forename: B
authors.initials: B
authors.lastname: Geny
authors.forename: D
authors.initials: D
authors.lastname: Segretain
authors.forename: E
authors.initials: E
authors.lastname: Turpin
authors.forename: A
authors.initials: A
authors.lastname: Alfsen
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D004023:Dictyostelium / Q000378:metabolism*; D018432:Drug Resistance, Multiple; D005110:Extracellular Space / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism*; D008970:Molecular Weight; D015262:Xenobiotics / Q000494:pharmacology
chemical_list: D001562:Benzimidazoles; D005456:Fluorescent Dyes; D015262:Xenobiotics; D004247:DNA; C017807:bisbenzimide ethoxide trihydrochloride