title: Cytotoxicity of endodontic materials.
abstract: An in vitro cell culture model of human gingival fibroblasts and L-929 cells was used to measure the cytotoxicity of currently used root canal sealers Endomet, CRCS, and AH26 and root-end filling materials Amalgam, Gallium GF2, Ketac Silver, mineral trioxide aggregate (MTA), and Super-EBA. Cytotoxic effects were assessed using the MTT assay for mitochondrial enzyme activity and the CV assay for cell numbers. Using inserts culture and L-929 fibroblasts. All-Bond-2 was also evaluated. The statistical analysis of results showed that CRCS was the least cytotoxic sealer followed by Endomet and AH26. Among root-end filling materials, MTA was not cytotoxic; Gallium GF2 displayed little cytotoxicity; and Ketac Silver, Super-EBA, and Amalgam showed higher levels of cytotoxicity. All Bond-2 also displayed a high degree of cytotoxicity. CRCS was the best root canal sealer and MTA the best root-end filling material. The outcome was favorable also for Gallium GF2 as a retrofilling material.
authors.affiliation: Department of Estomatoquirurgíca, University of Carabobo, Venezuela.
authors.forename: R M
authors.initials: RM
authors.lastname: Osorio
authors.forename: A
authors.initials: A
authors.lastname: Hefti
authors.forename: F J
authors.initials: FJ
authors.lastname: Vertucci
authors.forename: A L
authors.initials: AL
authors.lastname: Shawley
mesh_terms: D000497:Alloys / Q000633:toxicity; D017607:Aluminum Compounds / Q000633:toxicity; D000704:Analysis of Variance; D000818:Animals; D001729:Bismuth / Q000633:toxicity; D017610:Calcium Compounds / Q000633:toxicity; D002126:Calcium Hydroxide / Q000633:toxicity; D002478:Cells, Cultured; D016722:Cermet Cements / Q000633:toxicity; D003722:Dental Alloys / Q000633:toxicity; D017220:Dentin-Bonding Agents / Q000633:toxicity; D004338:Drug Combinations; D004853:Epoxy Resins; D005069:Evaluation Studies as Topic; D005347:Fibroblasts / Q000187:drug effects*; D005708:Gallium / Q000633:toxicity; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007739:L Cells; D008689:Methacrylates / Q000633:toxicity; D008709:Methenamine / Q000633:toxicity; D051379:Mice; D010087:Oxides / Q000633:toxicity; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity; D012834:Silver / Q000633:toxicity; D014025:Titanium / Q000633:toxicity; D015034:Zinc Oxide / Q000633:toxicity; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity
chemical_list: D000497:Alloys; D017607:Aluminum Compounds; D017610:Calcium Compounds; D016722:Cermet Cements; D003722:Dental Alloys; D017220:Dentin-Bonding Agents; D004338:Drug Combinations; C112829:Endomet; D004853:Epoxy Resins; D008689:Methacrylates; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C086631:mineral trioxide aggregate; C075563:Calcibiotic Root Canal Sealer; C098379:gallium alloy GF; C100742:All-Bond 2; D012834:Silver; C028044:epoxy resin AH-26; C084074:Super EBA; C072894:Valiant PhD; D005708:Gallium; D014025:Titanium; D008709:Methenamine; D002126:Calcium Hydroxide; D015034:Zinc Oxide; D001729:Bismuth