Binding of murine leukemia virus Gag polyproteins to KIF4, a microtubule-based motor protein.
A cDNA clone encoding a cellular protein that interacts with murine leukemia virus (MuLV) Gag proteins was isolated from a T-cell lymphoma library. The sequence of the clone is identical to the C terminus of a cellular protein, KIF4, a microtubule-associated motor protein that belongs to the kinesin superfamily. KIF4-MuLV Gag associations have been detected in vitro and in vivo in mammalian cells. We suggest that KIF4 could be involved in Gag polyprotein translocation from the cytoplasm to the cell membrane.
Kim
W
W
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892, USA.
Tang
Y
Y
Okada
Y
Y
Torrey
T A
TA
Chattopadhyay
S K
SK
Pfleiderer
M
M
Falkner
F G
FG
Dorner
F
F
Choi
W
W
Hirokawa
N
N
Morse
H C
HC
D000818:Animals; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D016547:Kinesins / Q000235:genetics / Q000378:metabolism*; D009052:Leukemia Virus, Murine / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
D015683:Gene Products, gag; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C107020:Pr60(gag) protein, murine AIDS virus; D011506:Proteins; D014764:Viral Proteins; C089655:Kif4 protein, mouse; D016547:Kinesins