Transfer reactions catalyzed by cyclodextrin glucosyltransferase using 4-thiomaltosyl and C-maltosyl fluorides as artificial donors.
Cyclodextrin glycosyltransferase enzyme from Bacillus circulans catalyzed the effective conversion of 4-thio-alpha-maltosyl fluoride into cyclo-alpha-(1-->4(2))-thiomalto -tetraoside, -pentaoside, -hexaoside and linear hemithiomaltooligosaccharides. However, under the same conditions, C-maltosyl fluoride afforded only linear modified maltotetraose, maltohexaose and maltooctaose in moderate yield.
Bornaghi
L
L
Centre de Recherches sur les Macromolécules Végétales, (CERMAV-CNRS), Grenoble, France.
Utille
J P
JP
Rekaï el-D
Mallet
J M
JM
Sinaÿ
P
P
Driguez
H
H
D001407:Bacillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D017611:Fluorine Compounds / Q000737:chemistry*; D005964:Glucosyltransferases / Q000378:metabolism*; D007700:Kinetics; D008320:Maltose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*
D017611:Fluorine Compounds; D009844:Oligosaccharides; C016549:maltohexaose; C101183:maltooctaose; D008320:Maltose; C009819:maltotetraose; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase