Simultaneous determination of maltose and glucose using a screen-printed electrode system.
A screen-printed sensor system consisting of a glucose oxidase (GOD) electrode and an amyloglucosidase/glucose oxidase (A/G) electrode was constructed to determine maltose and glucose simultaneously in a mixture. Sensor construction was optimised so that it contained 20 units of GOD/40 units of amyloglucosidase and 0.2 mM 1,1'-ferrocenedimethanol. These components were deposited onto a screen-printed carbon electrode and an outer membrane was printed from 3.5% hydroxyethyl cellulose (HEC) solution. The optimum pH was 4.8. The linear range of the system was up to 40 mM glucose or 20 mmol/L maltose with coefficients of variation (CVs) ranging from 3.5% to 5.29%. The results obtained by using the enzyme electrode system agreed well with those obtained by the Fehling titration method. When stored dry, especially at 4 degrees C, the enzyme electrodes showed good stability over four months.
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Wuhan Institute of Virology, Chinese Academy of Sciences, People's Republic of China.
Zhang
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Zhang
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Zhang
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XM
D015374:Biosensing Techniques; D002138:Calibration; D004563:Electrochemistry / Q000295:instrumentation*; D004579:Electron Transport; D005292:Ferricyanides; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase; D006863:Hydrogen-Ion Concentration; D016014:Linear Models; D008320:Maltose / Q000032:analysis*; D015203:Reproducibility of Results; D013997:Time Factors; D017186:Titrimetry
D005292:Ferricyanides; D008320:Maltose; D005949:Glucose Oxidase; D005947:Glucose; C028033:potassium ferricyanide