title: Structural characterization of a new acylpolyaminetoxin from the venom of Brazilian garden spider Nephilengys cruentata.
abstract: The use of mass spectrometry, in which high-energy CID and charge remote fragmentation both of protonated and sodium-attached molecular ions was applied, afforded the structural elucidation of a new acylpolyaminetoxin with Mw=801 Da from the venom of the Brazilian garden spider Nephilengys cruentata. In spite of having the same Mw of the NPTX-2, previously described in the venom of the Joro spider Nephila clavata, neither toxins are isomers. In order to differentiate them by using the most usual nomenclature, the new toxin was named NPTX-801C and the NPTX-2 was renamed to NPTX-801E. Both toxins have as common structure the 4-hydroxyindole-3-acetyl-asparaginyl-cadaveryl moiety in their molecules and their structure may be represented in a simplified way: NPTX-801E is HO-indole-Asn-Cad-Pta-Orn-Arg and NPTX-801C is HO-indole-Asn-Cad-Gly-Put-Pta-Pta.
authors.affiliation: Laboratory of Molecular Biology, Department of Biology, Institute of Biosciences, CEVAP-UNESP, Bela Vista, Rio Claro, SP, Brazil.
authors.forename: M S
authors.initials: MS
authors.lastname: Palma
authors.forename: Y
authors.initials: Y
authors.lastname: Itagaki
authors.forename: T
authors.initials: T
authors.lastname: Fujita
authors.forename: H
authors.initials: H
authors.lastname: Naoki
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001938:Brazil; D002851:Chromatography, High Pressure Liquid; D008970:Molecular Weight; D011073:Polyamines / Q000737:chemistry / Q000302:isolation & purification*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet; D013111:Spider Venoms / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; C114083:NPTX 801C; C114082:NPTX 801E; D011073:Polyamines; D013111:Spider Venoms