title: Histamine stimulates ion transport by dog pancreatic duct epithelial cells through H1 receptors.
abstract: Histamine affects pancreatic secretion, but its direct action on ion transport by pancreatic duct epithelial cells (PDEC) has not been defined. We now characterize the secretory effects of histamine on cultured, well-differentiated, and nontransformed dog PDEC. Histamine stimulated, in a concentration-dependent manner (1-100 microM), a cellular 125I- efflux that was inhibited by 500 microM 5-nitro-2-(3-phenylpropylamino)benzoic acid, 2.5 mM diphenylamine-2-carboxylate, and 500 microM DIDS and thus mediated through Ca2+-activated Cl- channels. Histamine-stimulated 125I- efflux was 1) inhibited by 100 microM diphenhydramine, an H1 receptor antagonist, 2) resistant to 1 mM cimetidine, an H2 receptor antagonist, 3) not reproduced by 1 mM dimaprit, an H2 agonist, and 4) inhibited by 50 microM 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-AM, a Ca2+ chelator, suggesting that it was mediated through H1 receptors acting via increased cytosolic Ca2+. Histamine also stimulated a 86Rb+ efflux that was sensitive to 100 nM charybdotoxin and thus mediated through Ca2+-activated K+ channels. When PDEC monolayers were studied in Ussing chambers, a short-circuit current of 21.7 +/- 3.1 microA/cm2 was stimulated by 100 microM histamine. This effect was inhibited by diphenhydramine but not cimetidine, was not reproduced with dimaprit, and was observed only after serosal addition of histamine, suggesting that it was mediated by basolateral H1 receptors on PDEC. In conclusion, histamine, acting through basolateral H1 receptors, activates both Ca2+-activated Cl- and K+ channels; in this manner, it may regulate PDEC secretion in normal or inflamed pancreas.
authors.affiliation: Department of Medicine, University of Washington, and Veterans Affairs Puget Sound Health Care System, Seattle, Washington 98108, USA.
authors.forename: T D
authors.initials: TD
authors.lastname: Nguyen
authors.forename: C N
authors.initials: CN
authors.lastname: Okolo
authors.forename: M W
authors.initials: MW
authors.lastname: Moody
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D002927:Cimetidine / Q000494:pharmacology; D017259:Dimaprit / Q000494:pharmacology; D004155:Diphenhydramine / Q000494:pharmacology; D004285:Dogs; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D006632:Histamine / Q000494:pharmacology*; D007454:Iodides / Q000378:metabolism; D007457:Iodine Radioisotopes; D007700:Kinetics; D009579:Nitrobenzoates / Q000494:pharmacology; D010183:Pancreatic Ducts / Q000502:physiology*; D011969:Receptors, Histamine H1 / Q000187:drug effects / Q000502:physiology*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
chemical_list: D002121:Calcium Channel Blockers; D002614:Chelating Agents; D002712:Chlorides; D007454:Iodides; D007457:Iodine Radioisotopes; D009579:Nitrobenzoates; D011969:Receptors, Histamine H1; D062367:ortho-Aminobenzoates; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; C085057:4-nitro-2-(3-phenylpropylamino)benzoate; D004533:Egtazic Acid; D002927:Cimetidine; D006632:Histamine; D004155:Diphenhydramine; C001209:fenamic acid; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D002118:Calcium; D017259:Dimaprit