Assembly of flammutoxin, a cytolytic protein from the edible mushroom Flammulina velutipes, into a pore-forming ring-shaped oligomer on the target cell.
Flammutoxin has been previously isolated as a cardiotoxic and cytolytic polypeptide of 22 or 32 kDa from the fruiting bodies of the edible mushroom Flammulina velutipes. In the present study, we purified flammutoxin as a single haemolytic protein of 31 kDa and studied the mode of its cytolytic action. (1) Flammutoxin caused efflux of potassium ions from human erythrocytes and swelling of the cells before haemolysis. (2) Flammutoxin did not lyse human erythrocytes in the presence of non-electrolytes with hydrodynamic diameters of >5.0 nm, although it caused leakage of potassium ions and swelling of the cells under the same conditions. (3) Experiments including solubilization of cell-bound toxin with 2% (w/v) SDS at 20 degrees C and subsequent Western immunoblots showed that flammutoxin formed a band corresponding to 180 kDa under the conditions where it lysed erythrocytes. (4) Electron microscopy of flammutoxin-treated human erythrocytes revealed the presence of a ring-shaped structure with outer and inner diameters of 10 and 5 nm, respectively, on the cells. (5) A ring-shaped toxin oligomer of the same dimensions was solubilized from the toxin-treated human erythrocytes with 2% (w/v) SDS at 20 degrees C and isolated by a sucrose-gradient ultracentrifugation. These data indicated that flammutoxin assembles into a ring-shaped oligomer possessing a hydrophilic pore of 4-5 nm on target cells.
Tomita
T
T
Laboratory of Culture Collection, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, Japan 108. tomita@biochem.tohoku.ac.jp
Ishikawa
D
D
Noguchi
T
T
Katayama
E
E
Hashimoto
Y
Y
D001487:Basidiomycota / Q000737:chemistry*; D001704:Biopolymers; D015153:Blotting, Western; D048429:Cell Size / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D006461:Hemolysis; D006801:Humans; D008854:Microscopy, Electron; D009183:Mycotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D011188:Potassium / Q000378:metabolism
D001704:Biopolymers; D005656:Fungal Proteins; D009183:Mycotoxins; C011823:FTX protein, Flammulina velutipes; D011188:Potassium