Effectiveness of SYTOX Green stain for bacterial viability assessment.
The effectiveness of SYTOX Green nucleic acid stain for measuring bacterial viability was tested on starved populations of Escherichia coli and Salmonella typhimurium. This stain underestimates the fraction of dead cells within starved populations containing cells with damaged nucleic acids or membranes. Its application to natural samples should be considered with caution.
Lebaron
P
P
Centre National de la Recherche Scientifique (CNRS-UMR7621), Institut National des Sciences de l'Univers et Université Paris VI, Banyuls-sur-Mer, France. lebaron@arago.obs-banyuls.fr
Catala
P
P
Parthuisot
N
N
D004396:Coloring Agents; D004926:Escherichia coli / Q000502:physiology*; D012486:Salmonella typhimurium / Q000502:physiology*
D004396:Coloring Agents