Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells.
We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
Cibelli
J B
JB
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003, USA.
Stice
S L
SL
Golueke
P J
PJ
Kane
J J
JJ
Jerry
J
J
Blackwell
C
C
Ponce de León
F A
FA
Robl
J M
JM
D000818:Animals; D030801:Animals, Genetically Modified; D001755:Blastocyst / Q000502:physiology*; D002417:Cattle / Q000235:genetics*; D002454:Cell Differentiation; D002459:Cell Fusion; D002678:Chimera; D004475:Ectoderm / Q000502:physiology; D004624:Embryo Transfer; D004707:Endoderm / Q000502:physiology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy; D008648:Mesoderm / Q000502:physiology; D013234:Stem Cells / Q000502:physiology*; D001616:beta-Galactosidase / Q000032:analysis
D001616:beta-Galactosidase