title: Sex differences in function and distribution of beta-adrenoceptors in rabbit urinary bladder.
abstract: OBJECTIVE
  To examine whether there are sex-related differences in urinary bladder function, we evaluated the function and distribution of beta-adrenoceptor in the male and female rabbit urinary bladder.
  
  
  METHODS
  Ten male and ten female rabbits were studied. The function and distribution of beta-adrenoceptors were studied by measuring changes in contractile forces from isoproterenol, a beta-adrenoceptor agonist, and forskolin, an adenylate cyclase activator, and by measuring beta-adrenoceptor densities using radioligand receptor binding techniques.
  
  
  RESULTS
  Isoproterenol caused significantly greater relaxation in female trigonal muscles than in male trigonal muscles. It caused similar relaxation responses in the male and female detrusors. The Bmax values for [3H]dihydroalprenolol (DHA) binding in trigonal muscles were significantly higher in the female than in the male, whereas Bmax values for [3H]DHA binding in the male and the female detrusor were similar. In contrast, forskolin caused similarly marked relaxations in the male and the female detrusor and trigonal muscles.
  
  
  CONCLUSIONS
  These findings demonstrate that there are sex-related differences in the function and the distribution of beta-adrenoceptors in rabbit trigonal smooth muscles.
authors.affiliation: Department of Urology, Tokyo Medical and Dental University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Morita
authors.forename: H
authors.initials: H
authors.lastname: Masuda
authors.forename: A
authors.initials: A
authors.lastname: Tosaka
authors.forename: K
authors.initials: K
authors.lastname: Ishizaka
authors.forename: T
authors.initials: T
authors.lastname: Tsujii
authors.forename: S
authors.initials: S
authors.lastname: Kondo
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D005576:Colforsin / Q000494:pharmacology; D005260:Female; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D012727:Sex Characteristics; D001743:Urinary Bladder / Q000737:chemistry*
chemical_list: D000318:Adrenergic beta-Agonists; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D007545:Isoproterenol