Alteration of acetohexamide reductase activities in kidney microsomes and cytosol of cadmium-treated rats.
We examined the alteration of acetohexamide reductase activities in kidney microsomes and cytosol of cadmium (Cd)-treated rats. Acetohexamide reductase activity in kidney microsomes of male rats was markedly decreased by treatment with Cd at a dose of 1.23 mg/kg body weight. However, the decreased enzyme activity was completely restored by repeated treatment with testosterone propionate. Therefore, it is reasonable to assume that the treatment with Cd indirectly affect the androgen-dependent acetohexamide reductase activity in kidney microsomes of male rats, possibly by depressing androgen production. In the case of female rats, unlike male rats, the microsomal enzyme activity was little detectable, and was unaffected by the treatment with Cd. Furthermore, Cd treatment had no significant effect on acetohexamide reductase activity in kidney cytosol of male or female rats.
Imamura
Y
Y
Faculty of Pharmaceutical Sciences, Kumamoto University, Japan. yorishig@gpo.kumamoto-u.ac.jp
Yamaguchi
S
S
Honda
Y
Y
Murata
H
H
Otagiri
M
M
D000429:Alcohol Oxidoreductases / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D003600:Cytosol / Q000201:enzymology; D005260:Female; D007668:Kidney / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology
D002104:Cadmium; D013739:Testosterone; D000429:Alcohol Oxidoreductases; C064152:acetohexamide reductase