title: Identification of linear DNA plasmids of the yeast Pichia pastoris.
abstract: Two DNA plasmids, approximately 11 and 8 kb in size, have been identified in a strain of the yeast Pichia pastoris (Northern Regional Research Laboratories No. Y4290). The plasmids are resistant to RNase A and lambda exonuclease, but are sensitive to digestion by DNase I, suggesting that they are linear and double-stranded DNA with 5'-protected ends. A restriction map has been constructed for the 11-kb plasmid, confirming that it is linear.
authors.affiliation: Department of Biology, Long Island University, Brooklyn Campus, New York, USA.
authors.forename: H
authors.initials: H
authors.lastname: Banerjee
authors.forename: C
authors.initials: C
authors.lastname: Kopvak
authors.forename: D
authors.initials: D
authors.lastname: Curley
mesh_terms: D004269:DNA, Bacterial / Q000737:chemistry / Q000302:isolation & purification*; D003850:Deoxyribonuclease I / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005092:Exonucleases / Q000737:chemistry; D010843:Pichia / Q000235:genetics*; D010957:Plasmids / Q000737:chemistry / Q000302:isolation & purification*; D015183:Restriction Mapping; D012259:Ribonuclease, Pancreatic / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005092:Exonucleases; D003850:Deoxyribonuclease I; D012259:Ribonuclease, Pancreatic