title: Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs.
abstract: A polymerase chain reaction (PCR) was optimized to detect Lawsonia intracellularis in faeces from naturally infected pigs. By combining a boiling procedure to extract DNA and a nested PCR procedure, a detection limit at 2 x 10(2) bacterial cells per gram of faeces was achieved. The optimized PCR was used together with conventional culture techniques to detect Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (WBHIS), Salmonella enterica, and haemolytic Escherichia coli, in a case control study to examine selected risk factors for the development of diarrhoea in growing pigs. Herds with diarrhoea were selected as cases and randomly chosen herds without diarrhoea were chosen as controls. Infection with L. intracellularis significantly enhanced the chance of diarrhoea. S. hyodysenteriae, WBHIS group IV (Serpulina pilosicoli), and S. enterica were isolated only from case herds which indicate that these species may influence the development of diarrhoea. In addition, herd-type had a significant impact, that is specific pathogen-free herds showed an odds ratio at 0.2 relative to conventional herds for the development of diarrhoea.
authors.affiliation: Danish Veterinary Laboratory, Copenhagen V, Denmark. krm@svs.dk
authors.forename: K
authors.initials: K
authors.lastname: Møller
authors.forename: T K
authors.initials: TK
authors.lastname: Jensen
authors.forename: S E
authors.initials: SE
authors.lastname: Jorsal
authors.forename: T D
authors.initials: TD
authors.lastname: Leser
authors.forename: B
authors.initials: B
authors.lastname: Carstensen
mesh_terms: D000818:Animals; D017401:Brachyspira hyodysenteriae / Q000302:isolation & purification; D016022:Case-Control Studies; D004247:DNA / Q000302:isolation & purification; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification / Q000472:pathogenicity; D005243:Feces / Q000382:microbiology; D005260:Female; D005767:Gastrointestinal Diseases / Q000382:microbiology / Q000662:veterinary*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D016017:Odds Ratio; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012044:Regression Analysis; D019779:Salmonella enterica / Q000145:classification / Q000302:isolation & purification; D013047:Specific Pathogen-Free Organisms; D013144:Spirochaetales / Q000145:classification / Q000302:isolation & purification*; D013145:Spirochaetales Infections / Q000382:microbiology / Q000662:veterinary*; D011795:Surveys and Questionnaires; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014774:Virulence
chemical_list: D004247:DNA