BC1 RNA protein particles in mouse brain contain two y-,h-element-binding proteins, translin and a 37 kDa protein.
Neural BC1 RNA is distributed in dendrites in the form of ribonucleoprotein particles (RNP). Recently, Han et al. reported that testis-brain RNA-binding protein (the mouse homologue of Translin), known to be a translational repressor, links a subset of mRNAs to microtubules (MTs) through binding to their y-,h-sequence elements. We found that similar elements are also present in BC1 RNA. Therefore, it is possible that they also act as cis-elements, together with their transacting proteins, and control the transport of BC1 RNA along dendritic MTs. In this study, we demonstrated that two y-,h-element-binding proteins copurified with BC1 RNP. One was identified as mouse Translin and the other was a 37 kDa protein. This macromolecular assembly may constitute a transport particle for BC1 RNA. It is also possible that BC1 RNP or Translin itself may play a regulatory role(s) in the translation of mRNAs within dendrites.
Muramatsu
T
T
Division of Molecular Cell Biology, College of Pharmacy, Nihon University, Chiba, Japan.
Ohmae
A
A
Anzai
K
K
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D022762:CCAAT-Enhancer-Binding Proteins; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008970:Molecular Weight; D011479:Protamines / Q000235:genetics; D012320:RNA Polymerase III / Q000235:genetics; D015347:RNA Probes; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000235:genetics; D020736:Ribonucleoproteins, Small Cytoplasmic
C076675:Bc1 long-non-coding RNA, mouse; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D011479:Protamines; D015347:RNA Probes; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D020736:Ribonucleoproteins, Small Cytoplasmic; C498303:Tsn protein, mouse; C068621:protamine 2; D012320:RNA Polymerase III