title: Molecular cloning of a novel GTP-binding protein induced in fish cells by rhabdovirus infection.
abstract: We have cloned and sequenced a cDNA encoding GTP-binding protein from a fish cell, CHSE-214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1-G5 that are conserved in all GTP-binding proteins. Its amino acid sequences are strikingly different from those of the well-characterized G-proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis, Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP-binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP-binding proteins which may play an important role in fish rhabdovirus infection.
authors.affiliation: Department of Molecular Biology, Pusan National University, South Korea.
authors.forename: E H
authors.initials: EH
authors.lastname: Lee
authors.forename: H J
authors.initials: HJ
authors.lastname: Kim
authors.forename: J J
authors.initials: JJ
authors.lastname: Park
authors.forename: J Y
authors.initials: JY
authors.lastname: Choi
authors.forename: W J
authors.initials: WJ
authors.lastname: Cho
authors.forename: S J
authors.initials: SJ
authors.lastname: Cha
authors.forename: C H
authors.initials: CH
authors.lastname: Moon
authors.forename: J M
authors.initials: JM
authors.lastname: Park
authors.forename: W J
authors.initials: WJ
authors.lastname: Yoon
authors.forename: B J
authors.initials: BJ
authors.lastname: Lee
authors.forename: D H
authors.initials: DH
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Kang
authors.forename: M A
authors.initials: MA
authors.lastname: Yoo
authors.forename: H D
authors.initials: HD
authors.lastname: Kim
authors.forename: J W
authors.initials: JW
authors.lastname: Park
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000821:virology*; D019204:GTP-Binding Proteins / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000662:veterinary*; D012474:Salmon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D019204:GTP-Binding Proteins