title: Structure and expression of an asparaginyl-tRNA synthetase gene located on chromosome IV of Arabidopsis thaliana and adjacent to a novel gene of 15 exons.
abstract: The gene AtNS1 coding for an asparaginyl-tRNA synthetase and located on chromosome IV of Arabidopsis thaliana has been characterized. AtNS1 is the first asparaginyl-tRNA synthetase gene described in higher plants. The genomic environment of AtNS1 has been studied, as well as a partial cDNA of a second homologous asparaginyl-tRNA synthetase gene, AtNS2. Both AtNS1 and AtNS2 exhibit the highest similarity with prokaryotic homologues. A large novel gene of 15 exons, named AtG2484-1, is located adjacent to AtNS1. AtG2484-1 shows features rarely described in plants including large exons and one 3' non-coding exon. PCR and Northern analyses were carried out to obtain information about the expression of these genes in various A. thaliana tissues.
authors.affiliation: Institut de Biotechnologie des Plantes, ERS/CNRS 569, Laboratoire de Biologie du Développement des Plantes, Bâtiment 630, Université de Paris-Sud, F-91405 Orsay Cedex, France.
authors.forename: S
authors.initials: S
authors.lastname: Aubourg
authors.forename: A
authors.initials: A
authors.lastname: Chéron
authors.forename: M
authors.initials: M
authors.lastname: Kreis
authors.forename: A
authors.initials: A
authors.lastname: Lecharny
mesh_terms: D000595:Amino Acid Sequence; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D001226:Aspartate-tRNA Ligase; D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant; D005091:Exons; D017343:Genes, Plant; D006801:Humans; D008969:Molecular Sequence Data; D012346:RNA, Transfer, Amino Acyl; D017386:Sequence Homology, Amino Acid
chemical_list: D018744:DNA, Plant; D012346:RNA, Transfer, Amino Acyl; D000604:Amino Acyl-tRNA Synthetases; D001226:Aspartate-tRNA Ligase; C029194:asparaginyl-tRNA synthetase