[Study of N-nitroso compounds which have NO-release ability].
Nitric oxide (NO), which plays an important role in the vital functions of organisms, is gaseous and labile molecule. Much attention has been paid to the stability and easily handling of NO donors, for careful handling of NO is required during experimental work. We synthesized a series of aromatic N-nitrosoureas and N-nitrosamides which efficiently liberates NO at room temperature. Generation of NO from the aromatic N-nitroso compounds was chemically confirmed by the trapping of NO as a nitrosyl complex of tetraphenylporphyrinatocobalt (II) and spectrophotometrically quantified by means of the Griess reaction using a newly designed test apparatus. 3,3-Dibenzyl-1-(4-tolyl)-1-nitrosourea showed the greatest NO-generating ability among the synthesized N-nitroso compounds. Further, the NO-generating ability was related to the reciprocal of the ID50 value for growth inhibition of cultured L-5178Y cell by the aromatic N-nitroso compounds.
Sueyoshi
S
S
Tanno
M
M
Miyata
N
N
D002455:Cell Division / Q000187:drug effects; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism*; D009603:Nitroso Compounds / Q000378:metabolism* / Q000494:pharmacology; D009607:Nitrosourea Compounds / Q000378:metabolism*
D009603:Nitroso Compounds; D009607:Nitrosourea Compounds; D009569:Nitric Oxide