title: Multiple sources of Escherichia coli O157 in feedlots and dairy farms in the northwestern USA.
abstract: Samples from cattle, other domestic and wild animals, flies, feeds, and water-troughs were collected from 12 cattle farms and tested for Escherichia coli O157. E. coli O157 was isolated from bovine fecal samples on all 12 farms with a within herd prevalence ranging from 1.1% to 6.1%. E. coli O157 was also found in 1 of 90 (1.1%) equine fecal samples, 2 of 65 (3.1%) canine fecal samples, 1 of 200 pooled bird samples (0.5%), 2 of 60 pooled fly samples (3.3%), and 10 of 320 (3.1%) water-trough sample sets (biofilm and water). No E. coli O157 were isolated from 300 rodents, 33 cats, 34 assorted wildlife, or 335 cattle feed samples. Indistinguishable pulsed-field gel electrophoresis patterns of XbaI digested chromosomal DNA and Shiga toxin types were observed for bovine and water-trough isolates from two farms and for one equine and two bovine isolates from one farm.
authors.affiliation: Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164-7060, USA. hancock@vetmed.wsu.edu
authors.forename: D D
authors.initials: DD
authors.lastname: Hancock
authors.forename: T E
authors.initials: TE
authors.lastname: Besser
authors.forename: D H
authors.initials: DH
authors.lastname: Rice
authors.forename: E D
authors.initials: ED
authors.lastname: Ebel
authors.forename: D E
authors.initials: DE
authors.lastname: Herriott
authors.forename: L V
authors.initials: LV
authors.lastname: Carpenter
mesh_terms: D000818:Animals; D002415:Cats / Q000382:microbiology; D002417:Cattle / Q000382:microbiology*; D003612:Dairying; D004175:Diptera / Q000382:microbiology; D004285:Dogs / Q000382:microbiology; D019453:Escherichia coli O157 / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D007061:Idaho; D009922:Oregon; D012377:Rodentia / Q000382:microbiology; D014861:Washington; D014881:Water Supply