title: DNA replication is completed in Saccharomyces cerevisiae cells that lack functional Cdc14, a dual-specificity protein phosphatase.
abstract: The Cdc14 protein encodes a dual-specificity protein phosphatase which functions in late mitosis, and considerable genetic evidence suggests a role in DNA replication. We find that cdc14 mutants arrested in late mitosis maintain persistent levels of mitotic kinase activity, suggesting that Cdc14 controls inactivation of this kinase. Overexpression of Sicl, a cyclin-dependent protein kinase inhibitor, is able to suppress telophase mutants such as dbf2, cdc5 and cdc15, but not cdc14. It does, however, force cdc14-arrested cells into the next cell cycle, in which an apparently normal S phase occurs as judged by FACS and pulsed-field gel electrophoretic analysis. Furthermore, in a promoter shut-off experiment, cells lacking Cdc14 appear to carry out a normal S phase. Thus Cdc14 functions mainly in late mitosis and it has no essential role in S phase.
authors.affiliation: Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, UK.
authors.forename: P J
authors.initials: PJ
authors.lastname: Fitzpatrick
authors.forename: J H
authors.initials: JH
authors.lastname: Toyn
authors.forename: J B
authors.initials: JB
authors.lastname: Millar
authors.forename: L H
authors.initials: LH
authors.lastname: Johnston
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D004261:DNA Replication; D004271:DNA, Fungal / Q000096:biosynthesis*; D004791:Enzyme Inhibitors / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology*; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000502:physiology; D017027:Protein Tyrosine Phosphatases; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013696:Temperature
chemical_list: C074860:CDC14 protein, S cerevisiae; D018797:Cell Cycle Proteins; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; C086926:SIC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases