Modification of tissue factor by peroxynitrite influences its procoagulant activity.
Peroxynitrite, a reactive oxidising species resulting from a reaction between nitric oxide and the superoxide anion, modifies proteins by nitration of certain amino acids such as tyrosine. Tissue factor (TF), a transmembrane protein, is expressed on cells under inflammatory conditions and initiates the coagulation cascade. The extracellular domain of TF is rich in tyrosine. Exposure of recombinant TF and cellular TF to peroxynitrite was associated with a reduction in procoagulant activity. This was accompanied by an elevated level of nitrotyrosine residues. Peroxynitrite may have a protective role by attenuation of the thrombotic properties of TF.
Adam
J M
JM
Department of Biochemistry and Molecular Biology, Royal Free Hospital School of Medicine, London, UK.
Ettelaie
C
C
Naseem
K M
KM
James
N J
NJ
Bradley
N J
NJ
Bruckdorfer
K R
KR
D003029:Coagulants / Q000737:chemistry / Q000378:metabolism*; D008565:Membrane Proteins / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects; D009566:Nitrates / Q000494:pharmacology*; D016877:Oxidants / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013925:Thromboplastin / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives
D003029:Coagulants; D008565:Membrane Proteins; D009566:Nitrates; D016877:Oxidants; D011994:Recombinant Proteins; C039642:peroxynitric acid; D014443:Tyrosine; D013925:Thromboplastin