Mutation analysis of the c-mos proto-oncogene in human ovarian teratomas.
Female transgenic mice lacking a functional c-mos proto-oncogene develop ovarian teratomas, indicating that c-mos may behave as a tumour-suppressor gene for this type of tumour. We have analysed the entire coding region of the c-MOS gene in a series of human ovarian teratomas to determine whether there are any cancer-causing alterations. DNA from twenty teratomas was analysed by single-strand conformational analysis (SSCA) and heteroduplex analysis (HA) to screen for somatic and germline mutations. In nine of these tumours the entire gene was also sequenced. A previously reported polymorphism and a single new sequence variant were identified, neither of which we would predict to be disease-causing alterations. These results suggest that mutations in the coding region of the c-MOS gene do not play a significant role in the genesis of human ovarian teratomas.
de Foy
K A
KA
CRC Human Cancer Genetics Research Group, Addenbrooke's Hospital, Cambridge, UK.
Gayther
S A
SA
Colledge
W H
WH
Crockett
S
S
Scott
I V
IV
Evans
M J
MJ
Ponder
B A
BA
D004252:DNA Mutational Analysis; D005260:Female; D016258:Genes, mos; D006801:Humans; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D000090063:Proto-Oncogene Mas; D013724:Teratoma / Q000235:genetics*