Induction of synaptosomal-associated protein-23 kD (SNAP-23) by various cytokines.
Cytokines manifest their function through regulation of gene expression. We searched for immediate-early cytokine responsive genes by the mRNA differential display technique using interleukin-3 (IL-3)-dependent OTT-1 cells, and have isolated a novel cDNA which encodes 210 amino acids and shows 87% amino acid identity to human SNAP-23 (synaptosomal-associated protein of 23 kD). The message for this protein (mouse SNAP-23) was induced in OTT-1 cells by IL-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-5. The experiment using C-terminal deletion mutants of the common beta subunit (betac) of IL-3/GM-CSF/IL-5 receptors showed that expression of SNAP-23 was associated with the Ras-Raf-MAPK pathway, but not with the JAK-STAT pathway. Moreover, SNAP-23 was induced in response to a wide variety of cytokines, including IL-2, IL-3, IL-5, IL-10, stem cell factor, G-CSF, GM-CSF, leukemia inhibitory factor, and erythropoietin. Constitutive expression of SNAP-23 was seen in various tissues, including heart, lung, kidney, liver, spleen, and small intestine. Possible involvement of SNAP-23 in cytokine signal transduction is discussed.
Morikawa
Y
Y
Department of Anatomy and Neurobiology, Wakayama Medical School, Wakayama, Japan.
Nishida
H
H
Misawa
K
K
Nosaka
T
T
Miyajima
A
A
Senba
E
E
Kitamura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics*; D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology*; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D007962:Leukocytes / Q000502:physiology*; D051379:Mice; D008969:Molecular Sequence Data; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D012333:RNA, Messenger / Q000032:analysis; D014018:Tissue Distribution
D002352:Carrier Proteins; D016207:Cytokines; D018076:DNA, Complementary; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D012333:RNA, Messenger; C100493:SNAP23 protein, human; C107017:Snap23 protein, mouse