title: Oligosaccharides derived by endo-beta-galactosidase digestion of bovine corneal keratan sulphate--characterisation of tetrasaccharides with incomplete sulphation and containing unsulphated N-acetylglucosamine residues.
abstract: Bovine corneal keratan sulphate has been fragmented using the enzyme endo-beta)-galactosidase and 1H-NMR chemical shift data are reported for five newly isolated tetrasaccharides which derived from the repeat region. They have the structures: GlcNAc(beta1-3)Gal(beta1-4)GlcNAc6S(beta1-3)Gal-ol, GlcNAc6S(beta1-3)Gal(beta1-4)GlcNAc(beta1-3)Gal-ol, GlcNAc(beta1-3)Gal6S(beta1-4)GlcNAc6S(beta1-3)Gal-ol, GlcNAc6S(beta1-3)Gal6S(beta1-4)GlcNAc(beta1-3)Gal-ol, and GlcNAc6S(beta1-3)Gal(beta1-4)GlcNAc6S(beta1-3)Gal-ol. Structures for two trisaccharides formed by a peeling reaction are also given. These are GlcNAc(beta1-3)Gal6S(beta1-4)GlcNAc-ol and GlcNAc6S(beta1-3)Gal(beta1-4)GlcNAc6S-ol where GlcNAc6S-ol represents N-acetylglucosaminitol 6-O-sulphate. Characterisation of such structures and their spectral assignments will be of considerable value for the studies of both selectin ligands and undersulphated keratan sulphates such as those occurring on cell surfaces and in brain tissue.
authors.affiliation: The Polymer Centre, School of Physics and Chemistry, Institute of Environmental and Natural Sciences, Lancaster University, England.
authors.forename: T N
authors.initials: TN
authors.lastname: Huckerby
authors.forename: G H
authors.initials: GH
authors.lastname: Tai
authors.forename: I A
authors.initials: IA
authors.lastname: Nieduszynski
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry* / Q000378:metabolism; D000606:Amino Sugars / Q000737:chemistry; D000818:Animals; D002417:Cattle; D003315:Cornea / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry; D006026:Glycoside Hydrolases; D007632:Keratan Sulfate / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry; D001616:beta-Galactosidase / Q000378:metabolism*
chemical_list: D000606:Amino Sugars; D006025:Glycosaminoglycans; D009844:Oligosaccharides; D011134:Polysaccharides; C020341:glucosaminoglycans; C039448:lactosaminoglycan; D007632:Keratan Sulfate; D006026:Glycoside Hydrolases; C026093:keratan-sulfate endo-1,4-beta-galactosidase; D001616:beta-Galactosidase; D000117:Acetylglucosamine