Immunohistochemical analysis of the expression of two serine-threonine kinases in the maturing mouse testis.
Previously we identified two intronless serine-threonine kinase genes (Tsk1 and Tsk2) located 3 kb apart on mouse chromosome 16 (Galili, N., Baldwin, H.S., Lund, J., Reeves, R., Gong, W., Wang, Z., Roe, B.A., Emanuel, B.S., Nayak, S., Mickanin, C., Budraf, M.L., Buck, C.A., 1997. A region of mouse chromosome 16 is syntenic to the DiGeorge, velocardiofacial syndrome minimal critical region. Gen. Res. 7, 17-26). Tsk1 was identical to a putative testicular kinase reported by Bielke et al. (Bielke, W., Blaschke, R.J., Miescher, G.C., Zurcher, G., Andres, A.C., Ziemiecki, A., 1994. Characterization of a novel murine testis-specific serine/threonine kinase. Gene 13, 235-239). Here we document the expression patterns of each Tsk throughout spermiogenesis showing an initial association of Tsk1 with cells in meiotic metaphase and a later association of Tsk2 with tail-like structures in the lumen of the seminiferous tubule.
Nayak
S
S
The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA.
Galili
N
N
Buck
C A
CA
D000818:Animals; D004790:Enzyme Induction; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008540:Meiosis / Q000235:genetics; D008677:Metaphase; D051379:Mice; D008856:Microscopy, Fluorescence; D017346:Protein Serine-Threonine Kinases / Q000096:biosynthesis* / Q000235:genetics; D012671:Seminiferous Tubules; D013082:Sperm Tail; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000201:enzymology* / Q000254:growth & development
C475219:Tsk1 protein, mouse; C475220:Tsk2 protein, mouse; D017346:Protein Serine-Threonine Kinases