Interleukin-10 reduces the endotoxin-induced hyperalgesia in mice.
In the endotoxin-induced inflammation, interleukin-10 reduced significantly, and in a dose-dependent manner, the inflammatory pain as assessed by mechanical and thermal tests. The levels of Tumour Necrosis Factor (TNF)alpha and NGF were upregulated at 1.5 h whereas those of IL-1beta at 6 h after ET injection. IL-10 downregulated the levels of TNFalpha (from 4974.75 +/- 875.78 to 1008 +/- 350 pg/hind paw), NGF (from 352.9 +/- 46.7 to 33.9 +/- 2.4 pg/hind paw) and IL-1beta (from 2773.88 +/- 423.96 to 1108 +/- 399.56 pg/hind paw). These data suggest that IL-10 inhibits ET-induced hyperalgesia by downregulation of TNFalpha, IL-1beta and NGF production.
Kanaan
S A
SA
Department of Biology, Faculty of Arts and Sciences, American University of Beirut, Lebanon. sk00@aub.edu.lb
Poole
S
S
Saadé
N E
NE
Jabbur
S
S
Safieh-Garabedian
B
B
D000818:Animals; D004731:Endotoxins / Q000494:pharmacology*; D006614:Hindlimb; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy* / Q000276:immunology*; D007375:Interleukin-1 / Q000032:analysis / Q000378:metabolism; D016753:Interleukin-10 / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009414:Nerve Growth Factors / Q000032:analysis / Q000378:metabolism; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D012867:Skin / Q000737:chemistry / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000378:metabolism
D004731:Endotoxins; D007375:Interleukin-1; D009414:Nerve Growth Factors; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10