title: Morphological examination of bone synthesis via direct administration of basic fibroblast growth factor into rat bone marrow.
abstract: Woven bone induced by direct injection of basic fibroblast growth factor (bFGF) into rat bone marrow was examined. On the first day after injection, fibrous tissues formed in the treated region of the bone marrow. Tissue-nonspecific alkaline phosphatase (TNAPase)-immunopositive osteoblastic cells and osteopontin immunopositive-extracellular matrices were observed in the fibrous tissues, indicating bone induction. On the fifth day, the bFGF-induced bone was found broadly in the bone marrow. In the originally existing bone, osteopontin-immunoreactivity was observed at cement lines, but not in the fully calcified matrix, whereas the woven bone displayed immunoreactivity throughout the matrix. Numerous TRAPase-positive osteoclasts were present on the surfaces of the woven bone, but no obvious cement line was observed. Therefore, both bone formation and resorption appeared highly active, without normal cellular coupling equilibrated between bone formation and resorption performed by osteoblasts and osteoclasts. On the tenth day, the bFGF-induced bone was almost replaced by bone marrow. Thus, bone formation actively occurred in the first half of the experimental period, whereas bone resorption came to be predominant thereafter. This study demonstrated that bFGF stimulates bone formation, which, however, is subject to subsequent resorption, probably due in part to the absence of coordinated cellular coupling between osteoclasts and osteoblasts.
authors.affiliation: 1st Department of Oral Anatomy, Niigata University School of Dentistry, Japan. amizuka@dent.niigata-u.ac.jp
authors.forename: N
authors.initials: N
authors.lastname: Amizuka
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: J I
authors.initials: JI
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Hoshi
authors.forename: M
authors.initials: M
authors.lastname: Fukushi
authors.forename: K
authors.initials: K
authors.lastname: Oda
authors.forename: Y
authors.initials: Y
authors.lastname: Ikehara
authors.forename: H
authors.initials: H
authors.lastname: Ozawa
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D001862:Bone Resorption; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D008854:Microscopy, Electron; D010006:Osteoblasts / Q000201:enzymology / Q000648:ultrastructure; D010010:Osteoclasts / Q000201:enzymology / Q000648:ultrastructure; D010012:Osteogenesis; D015676:Osteonectin / Q000235:genetics; D053495:Osteopontin; D051381:Rats; D017208:Rats, Wistar; D012795:Sialoglycoproteins / Q000032:analysis; D013997:Time Factors
chemical_list: D015676:Osteonectin; D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D016222:Fibroblast Growth Factor 2; D053495:Osteopontin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase