title: Ganglioside GM2 is substrate for a sialidase in MDCK cells.
abstract: GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.
authors.affiliation: Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Marchesini
authors.forename: G
authors.initials: G
authors.lastname: Benaglia
authors.forename: A
authors.initials: A
authors.lastname: Piccinotti
authors.forename: R
authors.initials: R
authors.lastname: Bresciani
authors.forename: A
authors.initials: A
authors.lastname: Preti
mesh_terms: D000818:Animals; D002460:Cell Line; D004285:Dogs; D005677:G(M1) Ganglioside / Q000378:metabolism; D005678:G(M2) Ganglioside / Q000378:metabolism*; D009439:Neuraminidase / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; D009439:Neuraminidase