[Use of silver sols as markers for immunoassay on microtiter plates].
Some basic characteristics of silver sols, facilitating their use as possible markers for the enzyme immunoassay on microtitration plates, were studied. Dispersion characteristics of silver sols obtained from AgNO3 solutions with a concentration of 0.005-0.5%, as well as the efficiency of their detection with physical developers on the basis of methol, paraphenylene diamine and amidol, were determined. The aggregative stability of the sol and the protective effect of a number of nonionic detergents, synthetic and biopolymeric, were evaluated. Similar sensitivity at the level of 1 ng/ml was attained in the comparative detection of lgG adsorbed on microtitration plates with the use of immunoperoxidase conjugate and silver immunosols. The possibility of using silver sols in the enzyme immunoassay as an alternative to enzymatic markers was shown.
Poltavchenko
A G
AG
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, Russia.
Karavaev
V S
VS
Tuzikov
F V
FV
D015415:Biomarkers; D003102:Colloids; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D012680:Sensitivity and Specificity; D012835:Silver Nitrate; D012996:Solutions; D017186:Titrimetry
D015415:Biomarkers; D003102:Colloids; D007074:Immunoglobulin G; D012996:Solutions; D012835:Silver Nitrate
Ispol'zovanie zoleĭ serebra kak merkerov immunoanaliza na mikrotitroval'nykh planshetakh.