SOR1, a gene required for photosensitizer and singlet oxygen resistance in Cercospora fungi, is highly conserved in divergent organisms.
Filamentous Cercospora fungi are resistant to photosensitizing compounds that generate singlet oxygen. C. nicotianae photosensitizer-sensitive mutants were restored to full resistance by transformation with SOR1 (Singlet Oxygen Resistance 1), a gene recovered from a wild-type genomic library. SOR1 null mutants generated via targeted gene replacement confirmed the requirement for SOR1 in photosensitizer resistance. SOR1 RNA is present throughout the growth cycle. Although resistance to singlet oxygen is rare in biological systems, SOR1, a gene with demonstrated activity against singlet-oxygen-generating photosensitizers, is highly conserved in organisms from widely diverse taxa. The characterization of SOR1 provides an additional phenotype to this large group of evolutionarily conserved genes.
Ehrenshaft
M
M
Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, USA.
Jenns
A E
AE
Chung
K R
KR
Daub
M E
ME
D003001:Cloning, Molecular; D017124:Conserved Sequence; D004271:DNA, Fungal / Q000032:analysis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005658:Fungi / Q000187:drug effects* / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism*; D010569:Perylene / Q000031:analogs & derivatives / Q000494:pharmacology; D010775:Photic Stimulation; D010777:Photochemistry; D017319:Photosensitizing Agents / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; C113268:PDX1 protein, Cercospora nicotianae; D017319:Photosensitizing Agents; D010569:Perylene; C026363:cercosporin; D010100:Oxygen