title: Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species.
abstract: 213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions on the following markers: CD1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43, 44, 45, 45R, 46, 49, 61, 62L, TCR gamma/delta, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new leucocyte antigen reactive mAbs.
authors.affiliation: Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
authors.forename: R
authors.initials: R
authors.lastname: Brodersen
authors.forename: F
authors.initials: F
authors.lastname: Bijlsma
authors.forename: K
authors.initials: K
authors.lastname: Gori
authors.forename: K T
authors.initials: KT
authors.lastname: Jensen
authors.forename: W
authors.initials: W
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Dominguez
authors.forename: K
authors.initials: K
authors.lastname: Haverson
authors.forename: P F
authors.initials: PF
authors.lastname: Moore
authors.forename: A
authors.initials: A
authors.lastname: Saalmüller
authors.forename: D
authors.initials: D
authors.lastname: Sachs
authors.forename: W J
authors.initials: WJ
authors.lastname: Slierendrecht
authors.forename: C
authors.initials: C
authors.lastname: Stokes
authors.forename: O
authors.initials: O
authors.lastname: Vainio
authors.forename: F
authors.initials: F
authors.lastname: Zuckermann
authors.forename: B
authors.initials: B
authors.lastname: Aasted
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D002415:Cats; D002417:Cattle; D002645:Chickens; D003429:Cross Reactions; D004285:Dogs; D006168:Guinea Pigs; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006736:Horses; D006801:Humans; D016023:Integrins / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008907:Mink; D008970:Molecular Weight; D017686:Oncorhynchus mykiss; D011233:Precipitin Tests; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D000949:Histocompatibility Antigens Class II; D016023:Integrins