Determination of ascorbic acid in food by column liquid chromatography with electrochemical detection using eluent for pre-run sample stabilization.
Determination of ascorbic acid (AA) in food was performed by column liquid chromatography with electrochemical detection using an eluent (100 mM KH2PO4 (pH 3) with 1 mM ethylenediaminetetraacetic acid disodium dihydrate) for pre-run sample stabilization. The applied potential was set at 400 mV vs. an Ag/AgCl reference electrode. The proposed method was simple, rapid (analysis time: ca. 8 min), sensitive (detection limit: ca. 0.5 ng per injection (20 microliters) at a signal-to-noise ratio of 3), highly selective and reproducible [relative standard deviation: ca. 1.8% (n = 5)]. The calibration graph for AA was linear in the range 0.1-16 ng per injection (20 microliters). Recovery of AA was over 90% by the standard addition method.
Iwase
H
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Ajinomoto Co., Central Research Laboratories, Kawasaki, Japan.
Ono
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D001205:Ascorbic Acid / Q000032:analysis*; D001628:Beverages / Q000032:analysis; D002853:Chromatography, Liquid; D002957:Citrus; D004563:Electrochemistry; D005504:Food Analysis / Q000379:methods*; D007202:Indicators and Reagents
D007202:Indicators and Reagents; D001205:Ascorbic Acid