Demonstration of receptors specific for connective tissue growth factor on a human chondrocytic cell line (HCS-2/8).
The presence of receptors specific for connective tissue growth factor (CTGF) was demonstrated on a human chondrosarcoma-derived chondrocytic cell line, HCS-2/8. The binding of 125I-labeled recombinant CTGF to HCS-2/8 cells was inhibited by unlabeled CTGF but not by PDGF-BB or bFGF. Scatchard analysis revealed the presence of two classes of binding sites with Kd values of 18.6 and 259 nM on cells. A cross-linking study revealed the formation of 125I-CTGF-receptor complex with an apparent molecular weight of 280 kDa. The 125I-CTGF-receptor complex disappeared almost completely on the addition of unlabeled CTGF but not PDGF-BB or bFGF. In addition, the 125I-CTGF-receptor complex was immunoprecipitated with anti-CTGF antiserum but not with anti-PDGF receptor antiserum. These findings suggest that CTGF directly binds to specific receptor molecules on HCS-2/8 cells.
Nishida
T
T
Department of Biochemistry and Molecular Dentistry, Okayama University Dental School, Japan.
Nakanishi
T
T
Shimo
T
T
Asano
M
M
Hattori
T
T
Tamatani
T
T
Tezuka
K
K
Takigawa
M
M
D001665:Binding Sites / Q000502:physiology; D001667:Binding, Competitive; D019902:Chondrocytes / Q000378:metabolism*; D055513:Connective Tissue Growth Factor; D003432:Cross-Linking Reagents / Q000378:metabolism; D006133:Growth Substances / Q000378:metabolism*; D006801:Humans; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes / Q000378:metabolism; D011233:Precipitin Tests; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000737:chemistry*; D011994:Recombinant Proteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
C526317:CCN2 protein, human; D003432:Cross-Linking Reagents; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D055513:Connective Tissue Growth Factor