title: A subset of notch functions during Drosophila eye development require Su(H) and the E(spl) gene complex.
abstract: The Notch signalling pathway is involved in many processes where cell fate is decided. Previous work showed that Notch is required at successive steps during R8 specification in the Drosophila eye. Initially, Notch enhances atonal expression and promotes atonal function. After atonal autoregulation has been established, Notch signalling represses atonal expression during lateral specification. In this paper we investigate which known components of the Notch pathway are involved in each signalling process. Using clonal analysis we show that a ligand of Notch, Delta, is required along with Notch for both proneural enhancement and lateral specification, while the downstream components Suppressor-of-Hairless and Enhancer-of-Split are involved only in lateral specification. Our data point to a distinct signal transduction pathway during proneural enhancement by Notch. Using misexpression experiments we also show that particular Enhancer-of-split bHLH genes can differ greatly in their contribution to lateral specification.
authors.affiliation: Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, and Department of Biology, University of Crete, Box 1527, GR 71110 Heraklion, Greece.
authors.forename: P
authors.initials: P
authors.lastname: Ligoxygakis
authors.forename: S Y
authors.initials: SY
authors.lastname: Yu
authors.forename: C
authors.initials: C
authors.lastname: Delidakis
authors.forename: N E
authors.initials: NE
authors.lastname: Baker
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins; D019521:Body Patterning; D002135:Calcium-Binding Proteins; D002454:Cell Differentiation; D002999:Clone Cells; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D004330:Drosophila / Q000254:growth & development; D029721:Drosophila Proteins; D005123:Eye / Q000254:growth & development*; D017344:Genes, Insect; D018257:Helix-Loop-Helix Motifs; D019476:Insect Proteins / Q000235:genetics*; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009419:Nerve Tissue Proteins; D017956:Photoreceptor Cells, Invertebrate / Q000254:growth & development; D051880:Receptors, Notch; D012097:Repressor Proteins / Q000235:genetics*; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000096:biosynthesis
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008024:Ligands; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D051880:Receptors, Notch; D012097:Repressor Proteins; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; C084530:E(spl)mdelta-HLH protein, Drosophila; C497759:N protein, Drosophila; C000606278:Ser protein, Drosophila; C088731:Su(H) protein, Drosophila; C082142:ato protein, Drosophila; C064045:delta protein