title: Salicylic acid induces a cytosolic Ca2+ elevation in yeast.
abstract: Cytosolic free calcium ion concentration ([Ca2+]cyt) after a salicylic acid (SA)-stimulus was monitored in cells of the yeast Saccharomyces cerevisiae expressing apoaequorin, which constitutes a Ca(2+)-sensitive luminescent protein, aequorin, when combined with coelenterazine. SA induced a transient [Ca2+]cyt elevation that was dependent on the concentration of SA and pH of the SA solution. The SA-induced [Ca2+]cyt elevation was not reduced in Ca(2+)-deficient medium, suggesting that Ca2+ was mobilized from an intracellular Ca2+ store(s). Benzoic acid, butyric acid and sorbic acid did not induced a [Ca2+]cyt elevation.
authors.affiliation: Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
authors.forename: I C
authors.initials: IC
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Iida
authors.forename: F I
authors.initials: FI
authors.lastname: Tsuji
authors.forename: M
authors.initials: M
authors.lastname: Isobe
authors.forename: N
authors.initials: N
authors.lastname: Uozumi
authors.forename: S
authors.initials: S
authors.lastname: Muto
mesh_terms: D000331:Aequorin / Q000378:metabolism*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects* / Q000378:metabolism; D008163:Luminescent Measurements; D010937:Plant Growth Regulators / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D012459:Salicylates / Q000494:pharmacology*; D020156:Salicylic Acid
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D010937:Plant Growth Regulators; D012459:Salicylates; D000331:Aequorin; D020156:Salicylic Acid; D002118:Calcium