Use of fluorescent-antibody staining of cytocentrifuge-prepared smears in combination with cell culture for direct detection of respiratory viruses.
Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA was 90%.
Doing
K M
KM
Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono 04469, USA. doing@maine.maine.edu
Jerkofsky
M A
MA
Dow
E G
EG
Jellison
J A
JA
D002478:Cells, Cultured; D002498:Centrifugation; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D009305:Nasopharynx / Q000821:virology; D010614:Pharynx / Q000821:virology; D011237:Predictive Value of Tests; D012137:Respiratory System / Q000821:virology*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis / Q000821:virology*; D014780:Viruses / Q000302:isolation & purification*