[The human SRY gene for prenatal diagnosis].
OBJECTIVE
To establish the method for prenatal sex diagnosis of the fetus carrying sex-linked genetic disorder.


METHODS
Human SRY gene was amplified by polymerase chain reaction. A 422-bp male specific fragment was obtained.


RESULTS
The fragment was identified in 10 men, but unidentified in 10 women. The diagnostic accordance rate of 20 amniotic fluid samples was 100%, 22 of 47 chorionic villi samples were positive. The rate of positive/negative (22/25) was nearly the sex rate of newborn babies. In the meantime, direct-PCR amplification of blood and amniotic fluid was completed. The fragment was shown from 4 microliters to 0.5 microliters of blood and from 2 ml to 0.5 ml of amniotic fluid.


CONCLUSIONS
The results show that fetal sex determination by PCR will be suitable for clinical prenatal diagnosis of sex-linked genetic disorders.
Gao
Y
Y
Molecular Biology Laboratory, Tianjin Third Center Hospital.
Ma
M
M
Min
L
L
D004268:DNA-Binding Proteins / Q000235:genetics; D005260:Female; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D006801:Humans; D008297:Male; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D012732:Sex Determination Analysis; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D014998:Y Chromosome
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors