Inhibition of gastric acid secretion by galanin in rats. Relation to endogenous histamine release.
Inhibitory effect of galanin on basal and secretagogs-stimulated gastric acid secretion was investigated in urethane-anesthetized rats. A rat stomach was mounted in an ex-vivo chamber, perfused with saline, and either gastric acid or alkaline secretion was determined by titrating the perfusate. Gastric mucosal blood flow (GMBF) was measured by a laser Doppler flowmeter. Intravenous infusion of galanin dose-dependently inhibited the increase of acid secretion induced by pentagastrin and carbachol but not by histamine, without any influence on the GMBF response. Galanin also reduced basal acid secretion while increasing GMBF, but did not evoke any change in basal gastric alkaline secretion. M15, which is a galanin receptor antagonist in the central nervous system but acts as a full agonist in the gastrointestinal smooth muscle, also suppressed pentagastrin-induced acid secretion, similar to galanin. In addition, pentagastrin increased the release of histamine into the gastric lumen, and this response was significantly inhibited by galanin. These results suggest that the inhibitory effect of galanin on acid secretion is mediated by suppression of endogenous histamine release from enterochromaffin-like cells and that the process may be related to the activation of the same subtype of galanin receptors as in the central nervous system and pancreatic beta-cells.
Kato
S
S
Department of Pharmacology and Experimental Therapeutics, Kyoto Pharmaceutical University, Yamashina, Japan. skato@mb.kyoto_phy.ac.jp
Korolkiewicz
R
R
Rekowski
P
P
Szyk
A
A
Sugawa
Y
Y
Takeuchi
K
K
D000818:Animals; D019004:Galanin / Q000031:analogs & derivatives / Q000494:pharmacology*; D005744:Gastric Acid / Q000378:metabolism*; D005753:Gastric Mucosa / Q000098:blood supply; D006636:Histamine Release / Q000187:drug effects*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide / Q000378:metabolism; D012039:Regional Blood Flow / Q000187:drug effects; D013373:Substance P / Q000031:analogs & derivatives / Q000494:pharmacology
D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide; C074975:galantide; D013373:Substance P; D019004:Galanin