title: Erythrocyte antioxidant enzyme activities in coal miners from three French regions.
abstract: This study investigated whether differences in the prevalence and severity of coal workers' pneumoconiosis (CWP) between three coal mines could be related to differences in oxidative stress exposure as evaluated in vivo through red-blood-cell antioxidant enzyme activities. Blood samples were obtained from 229 miners selected according to their occupation and their pneumoconiotic status. The following biomarkers were evaluated: erythrocyte catalase, Cu2+/Zn2+ superoxide dismutase (Cu2+/Zn2+ SOD), and glutathione peroxidase activities. Antioxidant enzyme activities did not differ significantly between the group of surface workers in Lorraine and the group of underground miners without CWP in Lorraine and in the other coal mines. Erythrocyte Cu2+/Zn2+ SOD activity was slightly decreased in the group of active underground miners with simple pneumoconiosis as compared with the group of miners without CWP in Nord/Pas-de-Calais. No effect was seen between retired miners at different stages of CWP. Our findings indicate that differences in the prevalence and severity of CWP do not seem to be related to various oxidative activities of coal dust particles, at least as reflected by measurements of antioxidant enzyme activities in circulating erythrocytes in this study.
authors.affiliation: INSERM U 420, Faculté de Médecine, Vandoeuvre-lès-Nancy, France. nadif@u420.nancy.inserm.fr
authors.forename: R
authors.initials: R
authors.lastname: Perrin-Nadif
authors.forename: J M
authors.initials: JM
authors.lastname: Porcher
authors.forename: M
authors.initials: M
authors.lastname: Dusch
authors.forename: J M
authors.initials: JM
authors.lastname: Mur
authors.forename: G
authors.initials: G
authors.lastname: Auburtin
mesh_terms: D000328:Adult; D000368:Aged; D000874:Anthracosilicosis / Q000175:diagnosis / Q000201:enzymology*; D015415:Biomarkers / Q000097:blood; D003032:Coal Mining; D004912:Erythrocytes / Q000201:enzymology*; D005602:France; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D015415:Biomarkers; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase