title: Two immunoglobulin-like domains of the Z-disc portion of titin interact in a conformation-dependent way with telethonin.
abstract: The giant muscle protein titin/connectin plays a crucial role in myofibrillogenesis as a molecular ruler for sarcomeric protein sorting. We describe here that the N-terminal titin immunoglobulin domains Z1 and Z2 interact specifically with telethonin in yeast two-hybrid analysis and protein binding assays. Immunofluorescence with antibodies against the N-terminal region of titin and telethonin detects both proteins at the Z-disc of human myotubes. Longer titin fragments, comprising a serine-proline-rich phosphorylation site and the next domain, do not interact. The interaction of telethonin with titin is therefore conformation-dependent, reflecting a possible phosphorylation regulation during myofibrillogenesis.
authors.affiliation: Structural Biology Division, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Mues
authors.forename: P F
authors.initials: PF
authors.lastname: van der Ven
authors.forename: P
authors.initials: P
authors.lastname: Young
authors.forename: D O
authors.initials: DO
authors.lastname: Fürst
authors.forename: M
authors.initials: M
authors.lastname: Gautel
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D064211:Connectin; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism*; D011487:Protein Conformation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D012518:Sarcomeres / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D064211:Connectin; D007136:Immunoglobulins; D009124:Muscle Proteins; C577292:TCAP protein, human; C577285:TTN protein, human; D011494:Protein Kinases