Detecting single base substitutions, mismatches and bulges in DNA by temperature gradient gel electrophoresis and related methods.
Temperature gradient gel electrophoresis (TGGE) and related methods can separate DNA fragments that differ by a single base pair or defect. This article describes the basic features of TGGE, and reviews the theoretical model of DNA unwinding and its ability to predict DNA mobility in a temperature gradient gel. Recent applications of TGGE and related methods that were directed at detecting point mutations, and evaluating the effects of single site defects are also reported.
Wartell
R M
RM
School of Biology, Georgia Institute of Technology, Atlanta 30332, USA.
Hosseini
S
S
Powell
S
S
Zhu
J
J
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D009691:Nucleic Acid Denaturation; D017354:Point Mutation; D011110:Polymorphism, Genetic; D013696:Temperature; D013816:Thermodynamics
D004247:DNA