title: Inhibition of Eimeria tenella development in vitro mediated by chicken macrophages and fibroblasts treated with chicken cell supernatants with IFN-gamma activity.
abstract: Pretreatment of chicken macrophages or fibroblasts with supernatants from concanavalin A-stimulated spleen cells or from the virus-transformed cell line reticuloendotheliovirus as source of interferon gamma (IFN-gamma) slows down subsequent sporozoite replication in the cells. To identify the presence of IFN-gamma, we combined four typical activities of IFN-gamma: inhibition of cytopathic effect of vesicular stomatitis virus on IFN-gamma-treated fibroblasts, cytostatic activity of IFN-gamma-activated macrophages, induction of major histocompatibility complex II antigen expression on IFN-gamma-activated fibroblasts and macrophages, and induction of nitrite production in macrophages. We have shown that chicken fibroblasts and macrophages possess a microbiostatic capacity once they are able to prevent the otherwise unchecked intracellular replication of Eimeria tenella following activation with culture supernatants identified as containing a strong IFN-gamma activity.
authors.affiliation: CJF INSERM 93-09 Immunologie des Maladies Infectieuses, Equipe associée INRA Immunologie Parasitaire UFR des Sciences Pharmaceutiques, Tours, France.
authors.forename: I H
authors.initials: IH
authors.lastname: Dimier
authors.forename: P
authors.initials: P
authors.lastname: Quéré
authors.forename: M
authors.initials: M
authors.lastname: Naciri
authors.forename: D T
authors.initials: DT
authors.lastname: Bout
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D016786:Eimeria tenella / Q000254:growth & development / Q000276:immunology*; D005347:Fibroblasts / Q000276:immunology* / Q000469:parasitology; D005434:Flow Cytometry / Q000662:veterinary; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis; D007371:Interferon-gamma / Q000032:analysis / Q000276:immunology*; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology; D009573:Nitrites / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms
chemical_list: D000949:Histocompatibility Antigens Class II; D009573:Nitrites; D007371:Interferon-gamma