Interactions of DNA with fluorescent dyes: by scanning tunneling microscopy.
Genomic DNA was obtained from peripheral blood samples of healthy volunteers and interacted with two fluorescent dyes (i.e. Hoechst 33,258 and ethidium bromide) in aqueous media. These media containing DNA-dye complexes deposited on the gold coated mica surfaces. Then, STM images were obtained in which the STM was operated in air at atmospheric pressure with a tip-to-substrate bias voltage of 250-1000 mV (sample positive) and the tunneling currents in the range of 10-20 pA by using etched tips of Pt/Ir, in constant current mode. Both dyes from molecular clusters on DNA. While, the Hoechst molecules were observed on the DNA chains at regular distances, the ethidium bromide molecular clusters did not.
Zareie
M H
MH
Chemical Engineering Department, Hacettepe University, Beytepe, Ankara, Turkey.
Sahin
F I
FI
Ergün
M A
MA
Koçum
C
C
Menevşe
S
S
Menevşe
A
A
Pişkin
E
E
D006690:Bisbenzimidazole; D004247:DNA / Q000097:blood / Q000737:chemistry* / Q000648:ultrastructure*; D004996:Ethidium; D005456:Fluorescent Dyes; D006801:Humans; D066298:In Vitro Techniques; D007364:Intercalating Agents; D046911:Macromolecular Substances; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D009690:Nucleic Acid Conformation; D010316:Particle Size
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D046911:Macromolecular Substances; D004247:DNA; D004996:Ethidium; D006690:Bisbenzimidazole