Expression of platelet-activating factor receptor transcript-2 is induced by shear stress in HUVEC.
Platelet-activating factor (PAF) is an important lipid involved in inflammation reaction and circulation regulation. The receptor for human PAF is synthesized from two spliced transcripts of the same gene. Our observation in the present study shows that HUVEC express transcript-1 only in the static condition. Shear stress induces the expression of transcript-2 in these cells but not transcript-1, resulting in increased PAF receptor expression as measured by FACS analysis. These results suggest that shear stress may increase the susceptibility of endothelial cells to PAF by inducing transcript-2 expression.
Okahara
K
K
Maryland Research Laboratory, Otsuka America Pharmaceutical Inc., Rockville 20850, USA. junichik@mrl.oapi.com
Sun
B
B
Kawasaki
T
T
Monden
M
M
Kambayashi
J
J
D017398:Alternative Splicing; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D010812:Physical Stimulation; D010980:Platelet Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D014471:Umbilical Veins
D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; C039351:platelet activating factor receptor