title: Gli2 is required for induction of floor plate and adjacent cells, but not most ventral neurons in the mouse central nervous system.
abstract: Induction of the floor plate at the ventral midline of the neural tube is one of the earliest events in the establishment of dorsoventral (d/v) polarity in the vertebrate central nervous system (CNS). The secreted molecule, Sonic hedgehog, has been shown to be both necessary and sufficient for this induction. In vertebrates, several downstream components of this signalling pathway have been identified, including members of the Gli transcription factor family. In this study, we have examined d/v patterning of the CNS in Gli2 mouse mutants. We have found that the floor plate throughout the midbrain, hindbrain and spinal cord does not form in Gli2 homozygotes. Despite this, motoneurons and ventral interneurons form in their normal d/v positions at 9.5 to 12.5 days postcoitum (dpc). However, cells that are generated in the region flanking the floor plate, including dopaminergic and serotonergic neurons, were greatly reduced in number or absent in Gli2 homozygous embryos. These results suggest that early signals derived from the notochord can be sufficient for establishing the basic d/v domains of cell differentiation in the ventral spinal cord and hindbrain. Interestingly, the notochord in Gli2 mutants does not regress ventrally after 10.5 dpc, as in normal embryos. Finally, the spinal cord of Gli1/Gli2 zinc-finger-deletion double homozygous mutants appeared similar to Gli2 homozygotes, indicating that neither gene is required downstream of Shh for the early development of ventral cell fates outside the ventral midline.
authors.affiliation: Developmental Genetics Program and Howard Hughes Medical Institute, and Department of Cell Biology and Physiology and Neuroscience, NYU Medical Center, New York, NY 10016, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Matise
authors.forename: D J
authors.initials: DJ
authors.lastname: Epstein
authors.forename: H L
authors.initials: HL
authors.lastname: Park
authors.forename: K A
authors.initials: KA
authors.lastname: Platt
authors.forename: A L
authors.initials: AL
authors.lastname: Joyner
mesh_terms: D000818:Animals; D019521:Body Patterning; D002454:Cell Differentiation; D002490:Central Nervous System / Q000196:embryology*; D003391:Cranial Nerves / Q000196:embryology; D004298:Dopamine; D004627:Embryonic Induction; D006720:Homozygote; D007395:Interneurons; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008817:Mice, Mutant Strains; D009046:Motor Neurons; D009672:Notochord / Q000196:embryology; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D012701:Serotonin; D013116:Spinal Cord / Q000196:embryology; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; D016335:Zinc Fingers
chemical_list: C000618551:Gli2 protein, mouse; D051741:Kruppel-Like Transcription Factors; D015513:Oncogene Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D000071676:Zinc Finger Protein GLI1; D000075746:Zinc Finger Protein Gli2; D012701:Serotonin; D004298:Dopamine