Evaluation of taurine metabolism in cats by dual stable isotope analysis.
Taurine kinetics in cats was investigated using a bolus dose of [15N]- and [1,2-13C2]taurine. The comparison of [15N]- and [13C2]taurine kinetics permitted an evaluation of the extent of taurine transamination. A methodology which involves N-pentafluorobenzoyl di-n-butylamine derivatization of taurine and GC/MS measurements of the 15N- and 13C-enrichments in cat urine was developed. Accuracy of the measurements was determined using pure standard compounds and the results showed that [13C2]taurine does not interfere with [15N]taurine. In cats, no differences were observed between both tracers. Therefore, we conclude that taurine reversible transamination does not occur at a significant level in cats.
Fay
L B
LB
Nestlé Research Centre (Friskies Research), Nestec Ltd., Lausanne, Switzerland. laurent.fay@chlsnr.nestrd.ch
Métairon
S
S
Montigon
F
F
Ballèvre
O
O
D000818:Animals; D002247:Carbon Isotopes; D002415:Cats; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes; D015203:Reproducibility of Results; D013654:Taurine / Q000378:metabolism* / Q000493:pharmacokinetics / Q000652:urine
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D013654:Taurine