Characterization of CD34+ thymic stromal cells located in the subcapsular cortex of the human thymus.
In this paper we report that suspensions of human fetal thymocytes contain cells that express high levels of CD34 and Thy-1. These cells were characterized with regard to location within the thymus, phenotype, and function. Confocal laser scan analysis of frozen sections of fetal thymus with anti-CD34 and Thy-1 antibodies revealed that the double-labeled cells were located in the pericortical area. In addition, it was found that the CD34+Thy-1+ cells lacked CD45 and CD50, indicating that these cells are not of hematopoietic origin; this was confirmed by the finding that these cells could be cultured as adherent cells in a medium with cholera toxin and dexamethasone, but failed to grow in mixtures of hematopoietic growth factors. Further analysis indicated that most cultured CD34+Thy-1+ cells expressed cytokeratin (CK) 14 but lacked CK 13, suggesting that these cells are immature epithelial cells. Cultured CD34+Thy-1+ cells were able to induce differentiation of CD1-CD34+CD3-CD4-CD8- thymic precursors into CD4+CD8+ cells in a reaggregate culture in the absence of exogenous cytokines. The CD4+CD8+ cells that developed in these cultures did not express CD3, indicating that CD34+Thy-1+ thymic stromal cells are not capable of completing full T cell differentiation of thymic hematopoietic progenitor cells.
Martínez-Cáceres
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Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Jaleco
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Res
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Noteboom
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Weijer
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Spits
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D002454:Cell Differentiation / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D018613:Microscopy, Confocal; D009924:Organ Culture Techniques; D017154:Stromal Cells / Q000276:immunology; D018800:Thy-1 Antigens / Q000032:analysis*; D013950:Thymus Gland / Q000166:cytology / Q000196:embryology / Q000276:immunology*
D018800:Thy-1 Antigens