Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase.
The behaviour of 5'-nucleotidase isoenzymes (ecto-5'-nucleotidase, e-Ns and c-N-II soluble 5'-nucleotidases) was studied in lymphocytes from patients with B-cell chronic lymphocytic leukemia. A strong reduction in ecto- and soluble activities was observed, although the pattern of the three 5'-nucleotidases did not always strictly overlap. A significant decrease (p<0.05) in ecto-5'-nucleotidase, e-Ns and c-N-II was found in B and T populations (B lymphocytes: 1.13, 0.88 and 1.26 nmol/h/10(6) cells versus 95.96, 9.64 and 13.73 nmol/h/10(6) cells in controls; T lymphocytes: 1.31, 0.23 and 0.06 nmol/h/10(6) cells versus 9.25, 1.31 and 2.10 nmol/h/10(6) cells in healthy subjects). The percentage of ecto-5'-nucleotidase-positive cells (CD73+) was reduced in leukemia patients, indicating a lower number of active molecules on the cell surface. The results of RT-PCR analysis showed that the ecto-5'-nucleotidase mRNA of leukemia patients was not defective.
Rosi
F
F
Institute of Biochemistry and Enzymology, University of Siena, Italy.
Agostinho
A B
AB
Carlucci
F
F
Zanoni
L
L
Porcelli
B
B
Marinello
E
E
Galieni
P
P
Tabucchi
A
A
D015720:5'-Nucleotidase / Q000097:blood*; D000241:Adenosine / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000368:Aged; D001402:B-Lymphocytes / Q000201:enzymology*; D016022:Case-Control Studies; D002450:Cell Communication / Q000502:physiology; D005260:Female; D006801:Humans; D006868:Hydrolysis; D007527:Isoenzymes / Q000097:blood*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D015398:Signal Transduction / Q000502:physiology; D013601:T-Lymphocytes / Q000201:enzymology*
D007527:Isoenzymes; D000255:Adenosine Triphosphate; D015720:5'-Nucleotidase; D000241:Adenosine