Sequence analysis and expression of Met-rich storage protein SP-1 of Hyphantria cunea.
We isolated and sequenced a cDNA clone corresponding to the 2.5 kb storage protein (SP-1) from fall webworm, Hyphantria cunea. The SP-1 gene encoded a pre-protein of 753 amino acids, including a signal peptide of 15 amino acids. The deduced amino acid sequence of SP-1 contained one potential N-glycosylation site, and the calculated isoelectric point and molecular weight of secreted SP-1 were pI = 8.38 and 86.8 kDa, respectively. A Northern blot of mRNA from various developmental stages revealed that the SP-1 mRNA in fat body appears in early last instar larvae and accumulates to a maximum level at the end of last instar larvae. The persistence of SP-1 transcript through the early pupal stage suggests that its mRNA might be stable or expressed during the pupal stage. SP-1 transcription was also found in the ovary as well as testis. This local expression of SP-1 in both reproductive organs seems to allow the insect to keep its reproductive activity under a nutritional stress.
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Department of Biology, College of Natural Sciences, Gyeongsang National University, Chinju, Korea.
Hwan
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Hwa
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Jae
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D005216:Fat Body / Q000737:chemistry; D015870:Gene Expression / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D007814:Larva / Q000737:chemistry; D008715:Methionine / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry / Q000235:genetics*; D011679:Pupa / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D019476:Insect Proteins; D012333:RNA, Messenger; C041137:storage proteins, Insecta; D008715:Methionine