title: Cold inactivation and dissociation into dimers of Escherichia coli tryptophanase and its W330F mutant form.
abstract: The kinetics and mechanism of reversible cold inactivation of the tetrameric enzyme tryptophanase have been studied. Cold inactivation is shown to occur slowly in the presence of K+ ions and much faster in their absence. The W330F mutant tryptophanase undergoes rapid cold inactivation even in the presence of K+ ions. In all cases the inactivation is accompanied by a decrease of the coenzyme 420-nm CD and absorption peaks and a shift of the latter peak to shorter wavelengths. The spectral changes and the NaBH4 test indicate that cooling of tryptophanase leads to breaking of the internal aldimine bond and release of the coenzyme. HPLC analysis showed that the ensuing apoenzyme dissociates into dimers. The dissociation depends on the nature and concentration of anions in the buffer solution. It readily occurs at low protein concentrations in the presence of salting-in anions Cl-, NO3- and I-, whereas salting-out anions, especially HPO4(2-), hinder the dissociation. K+ ions do not influence the dissociation of the apoenzyme, but partially protect holotryptophanase from cold inactivation. Thus, the two processes, cold inactivation of tryptophanase and dissociation of its apoform into dimers exhibit different dependencies on K+ ions and anions.
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Erez
authors.lastname: Gdalevsky GYa
authors.forename: Y M
authors.initials: YM
authors.lastname: Torchinsky
authors.forename: R S
authors.initials: RS
authors.lastname: Phillips
authors.forename: A H
authors.initials: AH
authors.lastname: Parola
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D003080:Cold Temperature; D019281:Dimerization; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D009154:Mutation; D011188:Potassium / Q000378:metabolism; D013057:Spectrum Analysis; D014368:Tryptophanase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014368:Tryptophanase; D011188:Potassium