Replication of bovine herpesvirus type 4 in human cells in vitro.
A reference strain (Movár 33/63) of bovine herpesvirus type 4 (BHV-4) was inoculated into 14 different human cell lines and five primary cell cultures representing various human tissues. BHV-4 replicated in two embryonic lung cell lines, MRC-5 and Wistar-38, and in a giant-cell glioblastoma cell culture. Cytopathic effect and intranuclear inclusion bodies were observed in these cells. PCR detected a 10,000-times-higher level of BHV-4 DNA. Titration of the supernatant indicated a 100-fold increase of infectious particles. Since this is the first bovine (human herpesvirus 8 and Epstein-Barr virus related) herpesvirus which replicates on human cells in vitro, the danger of possible human BHV-4 infection should not be ignored.
Egyed
L
L
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, Budapest. Laci@novell.vmri.hu
D000818:Animals; D002417:Cattle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis; D018142:Gammaherpesvirinae / Q000502:physiology*; D006801:Humans; D014407:Tumor Cells, Cultured; D014779:Virus Replication
D004279:DNA, Viral