A high-pressure, high-resolution NMR probe for experiments at 500 MHz.
A novel high-pressure, high-resolution NMR probe is described which operates at a frequency of 500 MHz. The design features an alternative RF coil (8 mm sample tube) for high frequency, sensitivity, probe power, and resolution (< 3.0 x 10(-9)). The probe is capable of pressures to at least 5 kbar over a temperature range of -30 to 80 degreesC, and has a double-tuned 1H/2H circuit which can tune at 1H frequencies of either 300 or 500 MHz. The sensitivity of the 300-MHz circuit is over twice that of previous 10-mm high-pressure NMR probe designs, while at 500 MHz the sensitivity is nearly five times that of previous 300-MHz pressure probes. Potential biochemical applications are demonstrated by 2D NOESY spectra of a Troponin C mutant.
Ballard
L
L
Department of Chemistry, School of Chemical Sciences, Urbana, Illinois, 61801, USA.
Yu
A
A
Reiner
C
C
Jonas
J
J
D003903:Deuterium; D004867:Equipment Design; D006801:Humans; D006859:Hydrogen; D007089:Image Enhancement / Q000295:instrumentation / Q000379:methods; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation* / Q000379:methods; D009154:Mutation / Q000235:genetics; D011312:Pressure; D013696:Temperature; D019209:Troponin C / Q000032:analysis / Q000235:genetics
D019209:Troponin C; D006859:Hydrogen; D003903:Deuterium