Nuclear antigens in Trichinella spiralis infected muscle cells: nuclear extraction, compartmentalization and complex formation.
Infection of mammalian skeletal muscle cells by Trichinella spiralis induces a series of changes that include: reentry of the terminally differentiated host cell into the cell cycle; suspension of infected cells in apparent G2/M; and transcriptional inactivation of the differentiated skeletal muscle gene program. Cell cycle repositioning and genetic reprogramming are chronic characteristics of host cells that can remain infected for years. Nuclear antigens (NA, 79, 86 and 97 kDa) that localize to host cell nuclei have been detected with antibodies against T. spiralis proteins. Since NA may play a role in regulating the infected cell phenotype, their origin, nuclear compartmentalization, and biochemical properties were investigated. We show that a monoclonal antibody to a defined epitope of T. spiralis glycans binds these NA, which indicates the parasite origin of these proteins. NA were not extracted under conditions that solubilized chromatin from infected cell nuclei. In contrast, NA were coextracted with B lamins (nuclear envelope) by 4 M urea. Urea extraction was pH dependent (8.0), suggesting ionic interaction of NA in protein complexes. Nevertheless, confocal microscopy demonstrated colocalization of NA with host chromatin, and not B lamins. Nuclear protein complexes containing NA were observed under non-reducing conditions, and NA were readily cross-linked in isolated nuclei by succinimidyl protein conjugating reagents. The results establish methods to extract NA from infected cell nuclei for further biochemical analysis, establish the existence of nuclear protein complexes containing NA and demonstrate colocalization of NA with host chromatin. Collectively, the results provide a foundation from which to investigate the role of NA in regulating the T. spiralis infected skeletal muscle cell phenotype.
Yao
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Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164-7040, USA.
Jasmer
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DP
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification; D002467:Cell Nucleus / Q000469:parasitology*; D002843:Chromatin; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000276:immunology / Q000502:physiology*; D006601:Hexoses / Q000032:analysis / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000469:parasitology*; D009685:Nuclear Envelope / Q000378:metabolism; D012995:Solubility; D017160:Trichinella spiralis / Q000276:immunology / Q000502:physiology*; D014235:Trichinellosis / Q000235:genetics / Q000276:immunology / Q000469:parasitology*; D014508:Urea
D000947:Antigens, Helminth; D002843:Chromatin; D000939:Epitopes; D015801:Helminth Proteins; D006601:Hexoses; C009616:tyvelose; D014508:Urea