[Assessment of the toxicity of TAH on the cell lines of SO-Rb50 and SO-Rb70].
OBJECTIVE
MTT assay was evaluated on cytoxicity for suspension growing cell lines of SO-Rb50 and SO-Rb70, and the toxicity of TAH (the total alkaloid of peqanum harmala L) on the above cell lines was assessed.


METHODS
The relationships between cell number and optical density, between optical density and exposure time of MTT, and the stability of formazan crystal solution in MDSO were determined. And the toxicity of TAH on the cell lines of SO-Rb50 and SO-Rb70 in vitro with MTT assay was assessed.


RESULTS
There was a direct proportional relationship between the amount of cell number and its optical density; The optical density increased gradully within 12 hours of the MTT incubation time; The stable time of the formazan crystal solved in DMSO was 11 hours. The IC50 values (micrograms/ml) of TAH on SO-Rb50 were 10.66, 4.82 respectively for 48 and 72 hours; and on SO-Rb70 were 6.38, 4.2 respectively for 48 and 72 hours.


CONCLUSIONS
MTT assay can be used for suspension growing cell lines of SO-Rb50 and SO-Rb70: TAH has obvious toxicity to these two cell lines.
Ping
Z
Z
Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou, China.
Feng
G
G
Yi
Y
Y
Wu
Z
Z
Li
Y
Y
Zheng
J
J
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor / Q000379:methods; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry*; D019572:Retinal Neoplasms / Q000473:pathology*; D012175:Retinoblastoma / Q000473:pathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic