The effects of TGF-beta1 on chick embryo retina development in vitro.
This paper studies the effect exerted by TGF-beta1 on the development of chick embryo retina cultured in vitro. The addition of TGF-beta1 to retinal explants inhibited DNA synthesis, measured as 3H-thymidine incorporation into trichloroacetic acid-insoluble fraction, while it increased both wet weight and protein content, in particular that of extracellular matrix proteins. Lastly, in explants treated with TGF-beta1 an increment in the level of fibronectin was demonstrated by means of Western blotting analysis.
Calvaruso
G
G
Institute of Biological Chemistry, University of Palermo, Italy.
Gerbino
E
E
Lauricella
M
M
Tesoriere
G
G
D000818:Animals; D015153:Blotting, Western; D002642:Chick Embryo / Q000502:physiology*; D046508:Culture Techniques; D004247:DNA / Q000037:antagonists & inhibitors; D004591:Electrophoresis, Polyacrylamide Gel; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D012160:Retina / Q000187:drug effects* / Q000196:embryology*; D013936:Thymidine / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D016212:Transforming Growth Factor beta; D004247:DNA; D013936:Thymidine