A study on the culture medium antigens of Cystcercus cellulosae for detecting antibodies of cysticercosis by means of ABC-ELISA.
Two antigens of Cysticercus cellulosae, cystic fluid antigen (CFA) and the culture medium antigen (CMA), were used in Avidin-Biotin Peroxdase Complex-ELISA (ABC-ELISA) to detect IgG antibodies in 45 cases of cysticercosis treated with praziquantel. The results revealed the total positive rates as 51.11% with CMA and 82.22% with CFA. The positive rates in the cases treated within 2 courses of treatment were 79.17% for CMA and 87.50% for CFA, and only 19.05% for CMA and 71.43% for CFA in the cases treated for more then 3 courses. The fact that the positive rates decreased as the courses of treatment increased showed that the sensitivity of CMA might be related to the vital conditions of the worms in the body, whether alive or dead. It is, therefore, recommended that CMA has the potential to be employed in ABC-ELISA both as an indicator for diagnosing cysticercosis and as a reference for the evaluation of the treatment.
Guo
H
H
Department of Parasitology, Hainan Medical College, Haikou, People's Republe of China.
Zhao
Z F
ZF
Shi
D Z
DZ
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D003470:Culture Media; D003551:Cysticercosis / Q000175:diagnosis* / Q000188:drug therapy / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D011223:Praziquantel / Q000627:therapeutic use; D016896:Treatment Outcome
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D003470:Culture Media; D007074:Immunoglobulin G; D011223:Praziquantel