[Insertion possibility of 16S-23S space amplification and random amplified polymorphic DNA analysis for typing of methicillin-resistant Staphylococcus aureus strains in the context of nosocomial infections].
Within the scope of the present study n = 183 MRSA isolates from the extended area of Düsseldorf and n = 93 international MRSA strains from seven different countries were typed by pulsed-field gel electrophoresis and two PCR methods (RAPD and 16S-23S-spacer amplification). The isolates could be subdivided into 30 different types by PFGE, into 21 by means of RAPD and 18 by 16S-23S-spacer amplification. PFGE had the highest discriminatory potential, however, a combined use of the three typing methods allows a more detailed differentiation even of those isolates with identical PFGE pattern. Both amplification procedures were rapid, easy in handling with reproductable results. For a temporary epidemiological analysis within 24 hours, both amplification methods could be combined. In case the investigated isolates were still suspected of showing a "clonal identity", they should be analysed by additional PFGE (lasting about four days). Although the international isolates were chosen by random selection, several MRSA strains with identical pattern could be found in different countries of the world. Some RAPD-, spacer- and PFGE pattern were constant over many years. This reflects a high genetic stability of single strains.
Schmitz
F J
FJ
Institut für Medizinische Mikrobiologie und Virologie der Heinrich-Heine-Universität Düsseldorf.
Jäger
B
B
Tichy
H V
HV
Idel
H
H
Hadding
U
U
Heinz
H P
HP
D003428:Cross Infection / Q000382:microbiology*; D004275:DNA, Ribosomal / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D006801:Humans; D016106:Methicillin Resistance; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D013203:Staphylococcal Infections / Q000145:classification / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S
Einsatzmöglichkeiten der 16S-23S-Spacer-Amplifikation und der "Random Amplified Polymorphic DNA"--Analyse zur Typisierung Methicillin-resistenter Staphylococcus aureus-Stämme im Rahmen nosokomialer Infektionen.