High-performance capillary electrophoretic characterization of different types of oligo- and polysialic acid chains.
We have carried out comparative structural analysis of novel oligo- and polysialic acid chains from diverse sources. Controlled acid hydrolysates of (a) colominic acid, alpha2-->8-linked homopolymer of N-acetylneuraminic acid (Neu5Ac), (b) alpha2-->8-linked oligo/polyNeu5Gc chains present in rainbow trout egg polysialoglycoprotein, and (c) alpha2-->8-linked oligomers of deaminoneuraminic acid (KDN) residues of KDN-rich glycoprotein derived from rainbow trout vitelline envelope were analyzed by high-performance capillary electrophoresis (HPCE). The results showed that three different types of alpha2-->8-linked oligosialic acids having same degree of polymerization can be separated by HPCE. A partial hydrolysate of colominic acid with mild acid was shown by CE to form intramolecular esters during the controlled hydrolysis and the subsequent workup procedure. In contrast, lactonization of (-->5-Oglycolyl-Neu5Gcalpha2-->)n, alpha2-->5-Oglycolyl-linked homopolymer of N-glycolylneuraminic acid (Neu5Gc) present in the egg jelly coat of sea urchin, did not take place as readily as in (-->8Neu5Acalpha2-->)n.
Cheng
M C
MC
Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei, 115, Taiwan.
Lin
S L
SL
Wu
S H
SH
Inoue
S
S
Inoue
Y
Y
D000818:Animals; D002236:Carbohydrate Conformation; D019075:Electrophoresis, Capillary / Q000379:methods; D005260:Female; D006868:Hydrolysis; D009844:Oligosaccharides / Q000737:chemistry*; D017686:Oncorhynchus mykiss; D010063:Ovum / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D012617:Sea Urchins; D012794:Sialic Acids / Q000032:analysis* / Q000737:chemistry*
D009844:Oligosaccharides; D011134:Polysaccharides; D012794:Sialic Acids; C021319:polysialic acid; C014420:colominic acid