title: Effect of dietary n-3 fatty acids on interleukin-2 and interleukin-2 receptor alpha expression in activated murine lymphocytes.
abstract: Dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) suppress interleukin-2 (IL-2) secretion and impair T-lymphocyte proliferation. To determine the mechanism of action, mice were fed diets containing either safflower oil (control diet enriched in linoleic acid, 18:2n-6), EPA, DHA or arachidonic acid (20:4n-6). Splenic lymphocytes were isolated and concanavalin A-induced kinetics of IL-2 and IL-2 receptor alpha mRNA expression were determined by relative competitive-PCR. EPA and DHA did not affect IL-2 mRNA expression but suppressed IL-2 receptor alpha mRNA levels. These data show, for the first time, the selective effects of dietary EPA and DHA on T-lymphocyte gene expression.
authors.affiliation: Faculty of Nutrition and Molecular and Cell Biology Group, Texas A&M University, College Station 77843-2471, USA.
authors.forename: C A
authors.initials: CA
authors.lastname: Jolly
authors.forename: D N
authors.initials: DN
authors.lastname: McMurray
authors.forename: R S
authors.initials: RS
authors.lastname: Chapkin
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004281:Docosahexaenoic Acids / Q000494:pharmacology; D015118:Eicosapentaenoic Acid / Q000494:pharmacology; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000235:genetics*; D012450:Safflower Oil / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D007376:Interleukin-2; D008934:Mitogens; D012333:RNA, Messenger; D015375:Receptors, Interleukin-2; D003208:Concanavalin A; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D012450:Safflower Oil; D015118:Eicosapentaenoic Acid