title: Interleukin-9 receptor alpha chain mRNA formation in CD8+ T cells producing anti-human immunodeficiency virus type 1 substance(s).
abstract: A search for gene(s) associated with anti-human immunodeficiency virus type 1 (HIV-1) activity of CD8+ T cells was attempted using molecular cloning and the relation between the anti-HIV activity of CD8+ T cells and the interleukin-9 receptor alpha chain (IL-9R-alpha) mRNA expression from the cDNA clones obtained was examined. The anti-HIV-1 activity of CD8+ T cell culture supernatants was assessed by measuring the level of HIV-1 replication of a CD4+ T cell line transfected with an infectious HIV-1 DNA clone. IL-9R-alpha mRNA was assayed by reverse transcriptase-polymerase chain reaction (RT-PCR). Of 5 cases showing high level of anti-HIV-1 activity (more than 80% suppression of HIV-1 replication), the mRNA was detected in 4 cases. Of 10 cases showing low level of anti-HIV-1 activity (less than 80% suppression of HIV-1 replication), the mRNA was detected in one case. Soluble recombinant human IL-9 receptor (rhIL-9sR) did not suppress HIV-1 replication at a concentration of 1 microgram/ml. These data suggest that the IL-9R-alpha mRNA formation in CD8+ T cells may correlate with and play some role in the anti-HIV-1 activity of CD8+ T cells from HIV-1-infected individuals.
authors.affiliation: Research Institute for Microbial Diseases, Osaka University, Japan.
authors.forename: M M
authors.initials: MM
authors.lastname: Hossain
authors.forename: H
authors.initials: H
authors.lastname: Tsuchie
authors.forename: M A
authors.initials: MA
authors.lastname: Detorio
authors.forename: H
authors.initials: H
authors.lastname: Shirono
authors.forename: C
authors.initials: C
authors.lastname: Hara
authors.forename: A
authors.initials: A
authors.lastname: Nishimoto
authors.forename: A
authors.initials: A
authors.lastname: Saji
authors.forename: J
authors.initials: J
authors.lastname: Koga
authors.forename: N
authors.initials: N
authors.lastname: Takata
authors.forename: J K
authors.initials: JK
authors.lastname: Maniar
authors.forename: D G
authors.initials: DG
authors.lastname: Saple
authors.forename: K
authors.initials: K
authors.lastname: Taniguchi
authors.forename: S
authors.initials: S
authors.lastname: Kageyama
authors.forename: H
authors.initials: H
authors.lastname: Ichimura
authors.forename: T
authors.initials: T
authors.lastname: Kurimura
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D012333:RNA, Messenger; D018123:Receptors, Interleukin / Q000235:genetics / Q000276:immunology*; D053651:Receptors, Interleukin-9
chemical_list: D018076:DNA, Complementary; C508045:IL9R protein, human; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D053651:Receptors, Interleukin-9