title: The visual cycle retinol dehydrogenase: possible involvement in the 9-cis retinoic acid biosynthetic pathway.
abstract: The 11-cis-retinol dehydrogenase (11-cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11-cis-retinol oxidation in the visual cycle. The structure of the murine 11-cis-RoDH gene was used to reinvestigate its transcription pattern. An 11-cis-RoDH gene transcript was detected in several non-ocular tissues. The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11-cis-RoDH was found capable of oxidizing and reducing 9-cis-, 11-cis- and 13-cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-beta-1-maltoside used to solubilize the enzyme was found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed.
authors.affiliation: Department of Ophthalmology, University of Nijmegen, The Netherlands. c.driessen@ohk.azn.nl
authors.forename: C A
authors.initials: CA
authors.lastname: Driessen
authors.forename: H J
authors.initials: HJ
authors.lastname: Winkens
authors.forename: E D
authors.initials: ED
authors.lastname: Kuhlmann
authors.forename: A P
authors.initials: AP
authors.lastname: Janssen
authors.forename: A H
authors.initials: AH
authors.lastname: van Vugt
authors.forename: A F
authors.initials: AF
authors.lastname: Deutman
authors.forename: J J
authors.initials: JJ
authors.lastname: Janssen
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D000077556:Alitretinoin; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D003315:Cornea / Q000201:enzymology*; D003360:Cosmids; D005091:Exons; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D051379:Mice; D008815:Mice, Inbred Strains; D009928:Organ Specificity; D010857:Pigment Epithelium of Eye / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D018411:Spodoptera; D013379:Substrate Specificity; D014158:Transcription, Genetic; D014162:Transfection; D014212:Tretinoin / Q000378:metabolism*; D014785:Vision, Ocular
chemical_list: D011994:Recombinant Proteins; D000077556:Alitretinoin; D014212:Tretinoin; D000429:Alcohol Oxidoreductases; C022520:retinol dehydrogenase