title: Electroporation and shock-induced transmembrane potential in a cardiac fiber during defibrillation strength shocks.
abstract: Experimental studies have shown that the magnitude of the shock-induced transmembrane potential (Vm) saturates with increasing electric field strength. This study uses a mathematical model to investigate the effects of electroporation and membrane kinetics on Vm in a cardiac fiber. The model consists of the core conductor equation for a one-dimensional fiber, where excitability is represented by the Luo-Rudy dynamic model (1994-1995) and electroporation is described by a membrane conductance that increases exponentially with Vm squared. For shocks delivered during the plateau of an action potential, the model reproduces the experimentally observed saturation of Vm with a root mean square error of 4.27% and a correlation coefficient of 0.9992. For shocks delivered during diastole, the saturation of Vm is qualitatively reproduced even when the sodium and calcium channels are inactivated. Quantitative replication of the response to diastolic shocks is hindered by the choice of electroporation parameters (optimized for shocks delivered during the plateau) and differences in the membrane kinetics between model and experiment. The complex behavior of Vm during large shocks is due to a combination of electroporation, electrotonus, propagation, and active membrane kinetics. The modeling results imply that the experimentally observed saturation of Vm is due to electroporation of the lipid bilayer.
authors.affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708-0281, USA. kad3@duke.edu
authors.forename: K A
authors.initials: KA
authors.lastname: DeBruin
authors.forename: W
authors.initials: W
authors.lastname: Krassowska
mesh_terms: D000200:Action Potentials; D000818:Animals; D001698:Biomedical Engineering; D002118:Calcium / Q000494:pharmacology; D003971:Diastole / Q000502:physiology; D004554:Electric Countershock; D004558:Electric Stimulation; D018274:Electroporation; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008955:Models, Cardiovascular; D010477:Perfusion; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D013779:Tetrodotoxin; D002118:Calcium