cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme.
A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.
Yanagisawa
K
K
Department of Biochemistry, University of Texas Health Center, Tyler 75710, USA.
Sakakibara
Y
Y
Suiko
M
M
Takami
Y
Y
Nakayama
T
T
Nakajima
H
H
Takayanagi
K
K
Natori
Y
Y
Liu
M C
MC
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents / Q000378:metabolism; D017931:DNA Primers; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008969:Molecular Sequence Data; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D013430:Sulfate Adenylyltransferase / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D003432:Cross-Linking Reagents; D017931:DNA Primers; D017853:Phosphotransferases (Alcohol Group Acceptor); C019617:adenylylsulfate kinase; D013430:Sulfate Adenylyltransferase