[In vitro study of hemopoietic progenitors in human umbilical cord blood treated with different cytokines and autologous serum].
The aim was to obtain the ex vivo expansion of human umbilical cord blood (HUCB) cells. A total of 19 samples were assayed to evaluate the number and type of hemopoietic progenitor cells, their proliferating capacity and the stimulating potency of cord blood serum.


METHODS
a) CFU-GM, CFU-GEMM and BFU-E cultures in the presence of CSF, BM serum or HUCB serum; b) 35 day LTC in liquid media whether in presence of IL-3 + GM-CSF + SCF or autologous serum (AS). Cells were demidepopulated at 7-day intervals and fresh medium and cytokines were added. Harvested cells were cultured in bone marrow (BM)/ SM and colonies were evaluated after 10 days.


RESULTS
The mean number of CFU-GM was similar to BM values; the maximum number of colonies was observed at day 7 and remained high until day 21 whether in addition of cytokines or AS. A total of 8 samples gave rise to colonies up to day 35; these samples showed higher values than BM in SM; HUCB serum has a great stimulating effect on BM cells and HUCB cells compared with nonspecific stimulating factors; moreover, HUCB showed a large dispersion.


CONCLUSIONS
1) HUCB contains a high number of hemopoietic progenitor cells with a large dispersion coefficient, 2) HUCB plasma has a great stimulatory capacity, 3) it is possible to induce the expansion of HUCB progenitors in LTC either in the presence of cytokines or of AS without loss of potency.
Alemán
M
M
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires.
Bianchi de Di Risio
C C
CC
D000704:Analysis of Variance; D018929:Cell Culture Techniques / Q000379:methods*; D016207:Cytokines; D006412:Hematopoietic Stem Cells; D006801:Humans; D007106:Immune Sera; D014470:Umbilical Cord
D016207:Cytokines; D007106:Immune Sera
Estudio in vitro de progenitores hemopoyéticos de sangre de cordón umbilical humano frente a diferentes citoquinas y suero autólogo.