Analysis of mechanisms of epidermal proliferation induced by intracutaneous injection of cholera toxin by the use of site-specifically mutated cholera toxins.
Intracutaneous injection of cholera toxin (CT) into rabbits increases vascular permeability and induces epidermal proliferation. To understand the mechanisms of these effects on the skin, we evaluated the involvement of the ADP-ribosyltransferase activity of the A subunit of CT and receptor-binding interactions between GM1-ganglioside and the B subunit of CT. We constructed two mutant CTs, E112K and W88K, by site-directed mutagenesis. Mutant CT-E112K, in which glutamic acid at position 112 (E112) of the A subunit of CT was replaced by lysine, has been shown to have lost its biological activity on Chinese hamster ovary (CHO) cells because of its abolished ADP-ribosyltransferase activity. Mutant CT-W88K, in which tryptophan at position 88 (W88) of the B subunit of CT was replaced by lysine, has been shown to have lost its binding ability to GM1-ganglioside. Intracutaneous injection of these mutant CTs evoked less vascular permeability and less epidermal proliferation than recombinant wild-type CT. These results suggest that: (1) the ADP-ribosyltransferase activity carried by E112 of the A subunit of CT; and (2) the binding ability to GM1-ganglioside via W88 of the B subunit of CT are essential for these effects of CT on the skin.
Yamaoka
J
J
Department of Dermatology, Kyoto University Hospital, Japan.
Imamura
S
S
D000818:Animals; D016466:CHO Cells; D002199:Capillary Permeability / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002772:Cholera Toxin / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology*; D006224:Cricetinae; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects*; D007271:Injections, Intradermal; D016297:Mutagenesis, Site-Directed; D009154:Mutation / Q000502:physiology; D011817:Rabbits; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D002772:Cholera Toxin