title: A mechanical and histomorphometric analysis of bone bonding by hydroxyapatite-coated strain gages.
abstract: Identification of the strains controlling bone remodeling is important for determining ways to prevent bone loss due to load deprivation, or implant placement. Long-term monitoring of strains can potentially provide the best information. Glues are resorbed within 2-3 weeks. Two formulations of microcrystalline hydroxyapatite (HA) were used to attach strain gages to rat femora to assess their long-term in vivo strain measurement capability. Seven male rats received HA-coated gages, and 2 animals underwent a sham procedure. The gages were prepared using a published technique and placed on the antero-lateral aspect of the left femora. After 6-7 weeks, the animals were euthanized and both femora explanted. Gages were attached to the right femora with cyanoacrylate. All femora were tested in cantilever bending, then embedded, sectioned, and stained with mineralized bone stain. The undecalcified sections were examined using transmitted and ultraviolet light microscopy. Mechanical testing showed one HA formulation provided 70-100% bonding. Histology showed intimate contact between the gage and bone surface. Histomorphometry indicated increased bone activity under the gage compared to the remaining bone, the controls, and the shams. The results indicate that microcrystalline HAs bond to bone quickly and can allow long term in vivo measurements.
authors.affiliation: Department of Surgery, Arizona Health Sciences Center, Tucson, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Wilson
authors.forename: J A
authors.initials: JA
authors.lastname: Szivek
authors.forename: P L
authors.initials: PL
authors.lastname: Anderson
authors.forename: V L
authors.initials: VL
authors.lastname: Miera
authors.forename: G A
authors.initials: GA
authors.lastname: Battraw
mesh_terms: D000818:Animals; D001843:Bone Cements; D001842:Bone and Bones; D017886:Durapatite; D008297:Male; D008422:Materials Testing; D010865:Pilot Projects; D019736:Prostheses and Implants; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical; D013499:Surface Properties
chemical_list: D001843:Bone Cements; D017886:Durapatite