title: p115 RhoGEF, a GTPase activating protein for Galpha12 and Galpha13.
abstract: Members of the regulators of G protein signaling (RGS) family stimulate the intrinsic guanosine triphosphatase (GTPase) activity of the alpha subunits of certain heterotrimeric guanine nucleotide-binding proteins (G proteins). The guanine nucleotide exchange factor (GEF) for Rho, p115 RhoGEF, has an amino-terminal region with similarity to RGS proteins. Recombinant p115 RhoGEF and a fusion protein containing the amino terminus of p115 had specific activity as GTPase activating proteins toward the alpha subunits of the G proteins G12 and G13, but not toward members of the Gs, Gi, or Gq subfamilies of Galpha proteins. This GEF may act as an intermediary in the regulation of Rho proteins by G13 and G12.
authors.affiliation: Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, TX 75235, USA.
authors.forename: T
authors.initials: T
authors.lastname: Kozasa
authors.forename: X
authors.initials: X
authors.lastname: Jiang
authors.forename: M J
authors.initials: MJ
authors.lastname: Hart
authors.forename: P M
authors.initials: PM
authors.lastname: Sternweis
authors.forename: W D
authors.initials: WD
authors.lastname: Singer
authors.forename: A G
authors.initials: AG
authors.lastname: Gilman
authors.forename: G
authors.initials: G
authors.lastname: Bollag
authors.forename: P C
authors.initials: PC
authors.lastname: Sternweis
mesh_terms: D017607:Aluminum Compounds / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D005459:Fluorides / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D044365:GTP-Binding Protein alpha Subunits, G12-G13; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction
chemical_list: D017607:Aluminum Compounds; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D011993:Recombinant Fusion Proteins; D006153:Guanosine Diphosphate; C050992:tetrafluoroaluminate; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D006160:Guanosine Triphosphate; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D044365:GTP-Binding Protein alpha Subunits, G12-G13; D005459:Fluorides