High-performance liquid chromatographic method for the determination of the major quinine metabolite, 3-hydroxyquinine, in plasma and urine.
The determination of 3-hydroxyquinine in urine and plasma samples is described. Extraction was performed using a mixture of toluene-butanol (75:25, v/v), followed by back-extraction into the mobile phase, which consisted of 0.1 M phosphate buffer, acetonitrile, tetrahydrofuran and triethylamine. A reversed-phase liquid chromatography system with fluorescence detection and a CT-sil C18 column were used. The within-assay coefficient of variation of the method was 2% at the higher concentration values in plasma, 2.95 microM, 4% at 227 nM and 9% at the lower limit of quantitation, 4.5 nM. In urine, the coefficient of variation was 11% at the lower concentration, 227 nM and was 3% at 56.8 microM. The between-assay coefficient of variation was 4% at the low concentration (5.1 nM) in plasma, 2% at 276.8 nM and 3% at 1.97 microM. In urine, the between assay coefficient of variation was 4% at 204.6 nM, 3% at 5.12 microM and 2% at 56.8 microM.
Mirghani
R A
RA
Department of Medical Laboratory Sciences and Technology, Karolinska Institute, Huddinge, Sweden.
Ericsson
O
O
Gustafsson
L L
LL
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D011802:Quinidine / Q000031:analogs & derivatives* / Q000097:blood / Q000652:urine; D011803:Quinine / Q000378:metabolism; D012680:Sensitivity and Specificity
C013569:3-hydroxyquinidine; D011803:Quinine; D011802:Quinidine