Streptococcus iniae, a human and animal pathogen: specific identification by the chaperonin 60 gene identification method.
It was recently reported that Streptococcus iniae, a bacterial pathogen of aquatic animals, can cause serious disease in humans. Using the chaperonin 60 (Cpn60) gene identification method with reverse checkerboard hybridization and chemiluminescent detection, we identified correctly each of 12 S. iniae samples among 34 aerobic gram-positive isolates from animal and clinical human sources.
Goh
S H
SH
Department of Pathology and Laboratory Medicine, University of British Columbia, and the Provincial Laboratory, British Columbia Centre for Disease Control Society, Vancouver, Canada. shgoh@unixg.ubc.ca
Driedger
D
D
Gillett
S
S
Low
D E
DE
Hemmingsen
S M
SM
Amos
M
M
Chan
D
D
Lovgren
M
M
Willey
B M
BM
Shaw
C
C
Smith
J A
JA
D000818:Animals; D015373:Bacterial Typing Techniques; D018834:Chaperonin 60 / Q000235:genetics*; D005069:Evaluation Studies as Topic; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D018834:Chaperonin 60