[Development of the technology of the purification of Coxiella burnetii from components of culture medium].
The technology of the purification of C.burnetii from the yolk-sac components of the cultivation medium, including differential centrifugation, membrane filtration, enzymatic hydrolysis, the standardization of the preparation thus obtained and its stabilization by the method of lyophilization, was developed. Antigens, prepared in accordance with this technology, were sufficiently active and highly specific. The possibility of their use in different serological tests (the complement fixation test and the indirect fluorescent analysis) was studied. In addition, these antigens were found to have good capacity for sorption on the solid-phase carrier (plates) and to be suitable for use in such highly sensitive test as the enzyme immunoassay.
Sosnina
O Iu
OIu
Pantiukhina
A N
AN
Petrov
V F
VF
Tupitsyn
A V
AV
D000818:Animals; D001709:Biotechnology; D002498:Centrifugation; D002642:Chick Embryo; D003168:Complement Fixation Tests; D016997:Coxiella burnetii / Q000302:isolation & purification*; D003470:Culture Media; D005374:Filtration; D019084:Fluorescent Antibody Technique, Indirect; D005612:Freeze Drying; D006868:Hydrolysis; D012680:Sensitivity and Specificity; D012698:Serologic Tests
D003470:Culture Media
Razrabotka tekhnologii ochistki Coxiella burnetii ot komponentov sredy kyl'tivirovaniia.