Short report: An imported case of cystic echinococcosis in Japan diagnosed by imaging and serology with confirmation of Echinococcus granulosus-specific DNA sequences.
We report one case of cystic echinococcosis (CE) in Japan in a native of Nepal. Ultrasonography and computed tomography scan of the liver revealed unique cystic lesions with or without daughter cysts of Echinococcus granulosus. Immunoblot analysis using crude antigens of E. multilocularis and cyst fluid of E. granulosus, without reference to these image analyses, strongly suggested this was a case of CE. We found protoscoleces in surgically removed hepatic lesions and analyzed the mitochondrial cytochrome c oxidase subunit I (COI) gene by the polymerase chain reaction. Based on the similarity in DNA sequences of the COI gene of this Echinococcus spp. with that of previously reported sheep-dog strain (GI), the parasite was considered to be the so-called common sheep strain of E. granulosus.
Ito
A
A
Department of Parasitology, Gifu University School of Medicine, Japan.
Okamoto
M
M
Ishiguro
T
T
Ma
L
L
Suzuki
H
H
Yasui
A
A
Shigeta
H
H
Matsuura
T
T
Hosokawa
T
T
Chai
J J
JJ
D000328:Adult; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D001483:Base Sequence; D018509:DNA, Helminth / Q000032:analysis / Q000737:chemistry; D004444:Echinococcosis, Hepatic / Q000175:diagnosis* / Q000469:parasitology / Q000601:surgery; D004446:Echinococcus / Q000201:enzymology / Q000235:genetics* / Q000276:immunology; D003576:Electron Transport Complex IV / Q000737:chemistry / Q000235:genetics; D006801:Humans; D007564:Japan; D008099:Liver / Q000000981:diagnostic imaging / Q000469:parasitology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D008969:Molecular Sequence Data; D009390:Nepal / Q000208:ethnology; D014057:Tomography, X-Ray Computed; D014463:Ultrasonography
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018509:DNA, Helminth; D003576:Electron Transport Complex IV