title: Nitric oxide synthase activity in arterial tissues of cirrhotic rats.
abstract: The changes in the activities of constitutive nitric oxide (NO) synthase (cNOS) and inducible NOS (iNOS) were investigated in arterial tissues of CCl4-induced cirrhotic adult SD rats. The aortic tissue homogenate were prepared in normal and cirrhotic rats. NOS activity was measured by conversion of 3H-arginine to 3 H-citrulline. The activities of cNOS and iNOS were calculated in terms of presence or absence of Ca2+. The results showed that activities of total NOS, cNOS and iNOS in arterial tissues were all increased significantly in cirrhotic rats as compared with those in normal controls. There was a significant positive correlation between the activities of total NOS and cGMP content in cirrhotic arterial tissues.
authors.affiliation: Institute of Liver Diseases, Tongji Hospital, Tongji Medical University, Wuhan.
authors.forename: P
authors.initials: P
authors.lastname: Zhang
authors.forename: K
authors.initials: K
authors.lastname: Liang
authors.forename: J
authors.initials: J
authors.lastname: Lin
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.forename: L
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002118:Calcium / Q000378:metabolism; D002252:Carbon Tetrachloride Poisoning; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D051381:Rats; D017207:Rats, Sprague-Dawley; D014664:Vasodilation
chemical_list: D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496318:Nos2 protein, rat; C496316:Nos3 protein, rat; D002118:Calcium