title: The Pumilio RNA-binding domain is also a translational regulator.
abstract: Posterior patterning in the Drosophila embryo requires the action of Nanos (Nos) and Pumilio (Pum), which collaborate to regulate the translation of maternal hunchback (hb) mRNA. Previous work demonstrated that Pum recognizes sites in the 3' UTR of hb mRNA. In this report, we first define the RNA-binding domain of Pum and then show that residues essential for translational repression are embedded within this domain. We also show that Nos and Pum can repress cap-independent translation from an internal ribosome entry site (IRES) in vivo, suggesting that they act downstream of the initial steps of normal, cap-dependent translation.
authors.affiliation: Howard Hughes Medical Institute, Department of Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA.
authors.forename: R P
authors.initials: RP
authors.lastname: Wharton
authors.forename: J
authors.initials: J
authors.lastname: Sonoda
authors.forename: T
authors.initials: T
authors.lastname: Lee
authors.forename: M
authors.initials: M
authors.lastname: Patterson
authors.forename: Y
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D005123:Eye / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D017344:Genes, Insect / Q000502:physiology; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000502:physiology; D010442:Peptide Chain Initiation, Translational; D011485:Protein Binding / Q000235:genetics; D014176:Protein Biosynthesis / Q000502:physiology*; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D012270:Ribosomes / Q000502:physiology
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; D016601:RNA-Binding Proteins; C074333:pum protein, Drosophila