title: Expression of cyclin D1 in mouse B cell lymphomas of different histologic types and differentiation stages.
abstract: The G1 cyclin, cyclin D1, has been implicated in the development of human and mouse tumors. Here we describe immunohistochemical analyses of cyclin D1 for a large panel of mouse B cell tumors. In addition, we characterize cyclin D1 expression in a series of cultured cell lines that represent transformed B cells at different stages of development. Immunohistochemical analysis showed that for low-grade lymphomas, cyclin D1 was expressed by 83% of centroblastic centrocytic (CBCC) and 14% of small lymphocytic lymphomas (SLL). For high-grade tumors, 28% of B lymphoblastic and 23% of centroblastic tumors expressed cyclin D1, while all immunoblastic lymphomas were negative. Studies of RNA and protein prepared from cultured B lineage tumors showed that cyclin D1 was expressed by all pre-B and most B cell tumors but not by cell lines representative of late B cell differentiation or by plasma cells. Expression of cyclin D1 in the lymphomas was not associated with alterations in the genomic structure of the Fis-1 (Bcl-1) common proviral integration site or cyclin D1 itself or with cell growth activity as assessed by expression of proliferating cell nuclear antigen (PCNA).
authors.affiliation: Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-0760, USA.
authors.forename: C F
authors.initials: CF
authors.lastname: Qi
authors.forename: S K
authors.initials: SK
authors.lastname: Chattopadhyay
authors.forename: M
authors.initials: M
authors.lastname: Lander
authors.forename: Y
authors.initials: Y
authors.lastname: Kim
authors.forename: T N
authors.initials: TN
authors.lastname: Fredrickson
authors.forename: J W
authors.initials: JW
authors.lastname: Hartley
authors.forename: H C
authors.initials: HC
authors.lastname: Morse
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002454:Cell Differentiation; D019938:Cyclin D1 / Q000235:genetics*; D004247:DNA / Q000032:analysis; D035021:Factor For Inversion Stimulation Protein; D015870:Gene Expression; D016259:Genes, myc / Q000235:genetics; D007150:Immunohistochemistry; D034982:Integration Host Factors; D016393:Lymphoma, B-Cell / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D016399:Lymphoma, T-Cell / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D019938:Cyclin D1; D004247:DNA