Entry of African swine fever virus into Vero cells and uncoating.
African swine fever virus (ASFV) enters Vero cells by adsorptive endocytosis [Valdeira, M.L., Geraldes, A., 1985. Morphological study on the entry of African swine fever virus into cells, Biol Cell. 55, 35-40]. Electron microscopy of a lysosomotropic drug-controlled penetration indicated that this step takes place in the endosomes, after fusion between the viral envelope and the limiting membrane of the endosome. Inhibition studies with colcemid, cytochalasin B, sodium azide, dinitrophenol, lysosomotropic weak bases, and the ionophore monensin, showed that the virus uptake is largely independent of cytoskeletal and lysosomal function, but dependent on oxidative phosphorylation. Some protease inhibitors inhibited viral replication at an early step, indicating that the initiation of infection depends on a viral proteolytic cleavage.
Valdeira
M L
ML
Department of Cellular Biology, Faculty of Pharmacy, Lisboa, Portugal. lvaldeira@ff.ul.pt
Bernardes
C
C
Cruz
B
B
Geraldes
A
A
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000327:Adsorption; D000358:African Swine Fever Virus / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D002522:Chlorocebus aethiops; D002738:Chloroquine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D003703:Demecolcine / Q000494:pharmacology; D004705:Endocytosis; D011992:Endosomes / Q000648:ultrastructure / Q000821:virology; D008247:Lysosomes / Q000187:drug effects / Q000648:ultrastructure / Q000821:virology*; D008561:Membrane Fusion; D008985:Monensin / Q000494:pharmacology; D019810:Sodium Azide / Q000494:pharmacology; D014709:Vero Cells / Q000648:ultrastructure / Q000821:virology
D000643:Ammonium Chloride; D003571:Cytochalasin B; D002738:Chloroquine; D008985:Monensin; D019810:Sodium Azide; D019297:2,4-Dinitrophenol; D003703:Demecolcine