Expression of mRNA for phospholipase A2, cyclooxygenases, and lipoxygenases in cultured human umbilical vascular endothelial and smooth muscle cells and in biopsies from umbilical arteries and veins.
Arachidonic acid (AA) is released by phospholipase A2 (PLA2) and then converted into vasoactive and inflammatory eicosanoids by cyclooxygenases (COX) and lipoxygenases (LOX). These eicosanoids are important paracrine regulators of vascular permeability, blood flow, local pro- and anticoagulant activity and they play a major role in the local inflammatory response. We have investigated the presence of mRNAs for PLA2 and for isoforms of COX and LOX in both human endothelial cells (EC) and in human smooth muscle cells (SMC) in culture and in vascular biopsies of human umbilical veins (HUVB) and arteries (HUAB) by using the reversed transcription-polymerase chain reaction (RT-PCR) technique. Results show detectable levels of PLA2 type IV (cPLA2) in cultured EC and SMC and in vascular wall biopsies from HUAB and HUVB. The cultured EC and SMC demonstrate higher levels of both COX-1 and COX-2 with PCR analyses than do vascular wall biopsies from HUAB and HUVB. This indicates a difference in the native expression of COX-1 and COX-2 in cultures of EC and SMC compared to that in biopsies from intact vessel walls. The EC and SMC in culture do not express mRNA for 5-LOX, that was, however, expressed in the vascular wall biopsies. This speaks in favour of a constitutive, i.e. in vivo expression of 5-LOX in SMC in the vascular wall of both umbilical vein and arteries. Thus results from in vitro studies of constitutive COX and LOX expression in EC and vascular SMC in culture cannot simply be extrapolated to represent in vivo conditions.
Ost
M
M
Clinical Research Center, University of Linköping, Sweden.
Uhl
E
E
Carlsson
M
M
Gidlöf
A
A
Söderkvist
P
P
Sirsjö
A
A
D001706:Biopsy; D002478:Cells, Cultured; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D003600:Cytosol / Q000737:chemistry / Q000201:enzymology; D004730:Endothelium, Vascular / Q000166:cytology / Q000201:enzymology; D015870:Gene Expression / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008084:Lipoxygenase / Q000235:genetics*; D008565:Membrane Proteins; D008970:Molecular Weight; D009130:Muscle, Smooth / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D010105:Oxygenases / Q000235:genetics; D010741:Phospholipases A / Q000235:genetics*; D054467:Phospholipases A2; D016133:Polymerase Chain Reaction; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D014018:Tissue Distribution; D014469:Umbilical Arteries / Q000737:chemistry / Q000201:enzymology / Q000473:pathology*; D014471:Umbilical Veins / Q000737:chemistry / Q000201:enzymology / Q000473:pathology*
D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D010105:Oxygenases; D008084:Lipoxygenase; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054467:Phospholipases A2