Simple microbore high-performance liquid chromatographic method for the determination of dopamine and cocaine from a single in vivo brain microdialysis sample.
A microbore chromatographic method for the analysis of both dopamine and cocaine from in vivo brain microdialysis samples is described. To eliminate the need for separate chromatographic systems for each analyte, post-column electrochemical and ultraviolet detection systems were arranged in series. The limit of quantitation for dopamine (5 fmol) was well within range for detecting dialysate concentrations of this neurotransmitter in rats which were in a baseline, drug-free state. The limit of quantitation for cocaine (0.5 pmol) was sufficient to detect brain cocaine levels following the peripheral administration of a low dose of this psychostimulant (5 mg/kg, i.p.). Estimates of dialysate dopamine and cocaine concentrations after 5, 10 and 20 mg/kg cocaine (i.p.) were in agreement with reports which utilized separate HPLC analyses for each analyte.
Parsons
L H
LH
Department of Neuropharmacology, CVN-7, The Scripps Research Institute, La Jolla, CA 92037, USA.
Kerr
T M
TM
Weiss
F
F
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003042:Cocaine / Q000302:isolation & purification*; D004298:Dopamine / Q000302:isolation & purification*; D008297:Male; D017551:Microdialysis; D009714:Nucleus Accumbens / Q000737:chemistry; D051381:Rats
D003042:Cocaine; D004298:Dopamine