[Phase transition in the matrix as a regulator of enzymatic activity of proteinases].
The kinetic behavior of proteolytic enzymes immobilized in thermosensitive hydrogels was studied at the phase transition (collapse) of the carriers. The dependence of the activity of immobilized enzymes on the state of the matrix allows the use of the hydrogel phase transition to regulate the activity of immobilized enzymes. Several cases of such regulation were demonstrated.
Eremeev
N L
NL
Department of Chemical Enzymology, Chemistry Faculty, Moscow State University, Russia.
Kazanskaia
N F
NF
D000180:Acrylic Resins / Q000737:chemistry*; D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D010450:Endopeptidases / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005782:Gels; D006863:Hydrogen-Ion Concentration; D013379:Substrate Specificity; D013696:Temperature; D014510:Urease / Q000737:chemistry / Q000378:metabolism
D000180:Acrylic Resins; D004800:Enzymes, Immobilized; D005782:Gels; C052970:poly-N-isopropylacrylamide; D000469:Alkaline Phosphatase; D010450:Endopeptidases; D002918:Chymotrypsin; D014510:Urease
Fazovyĭ perekhod v matritse kak reguliator fermentativnoĭ aktivnosti proteinaz.