Effects of cyclosporin A on proliferation of cultured rat mesangial cells.
The proliferation of mesangial cells on cyclosporin (CsA) test medium was studied by MTT assay and TNF-alpha in cultured supernatant was examined by using ELISA. The results showed that cyclosporin A significantly inhibited the proliferation of mesangial cells at the concentration between 0.25-15 micrograms/ml (IC50 1 microgram). This action appeared to be dose-dependent. Release of TNF-alpha from mesangial cells stimulated by LPS was also dose-dependently suppressed. It is suggested that cyclosporin A play an important role in antiproliferation mechanism of mesangial cells in vitro.
Sun
J
J
Department of Pediatrics, Tongji Medical University, Wuhan.
Wang
Y
Y
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016572:Cyclosporine / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology / Q000187:drug effects*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D007166:Immunosuppressive Agents; D014409:Tumor Necrosis Factor-alpha; D016572:Cyclosporine