Taenia crassiceps cysticercosis: humoral immune response and protection elicited by DNA immunization.
The purpose of this study was to evaluate DNA vaccination in cysticercosis prevention by using a Taenia crassiceps cDNA of a recombinant antigen (KETc7) that has been reported as protective against murine cysticercosis. The KETc7 cDNA was cloned into the pcDNA3 plasmid alone or with the betaglycan signal peptide sequence (pTc-7 and pTc-sp7, respectively). Positive expression of the pTc-sp7 product was confirmed by transfection of C33 cells and immunofluorescence using sera of mice infected with T. crassiceps. Immunization of mice with 3 injections of pTc-sp7 DNA at the higher dose (200 microg) was the most effective to induce antibody with or without bupivacaine. Immunization with pTc-sp7 induced protection against challenge with T. crassiceps cysticerci as successfully as previously observed with the KETc7 recombinant protein. Antibodies elicited by DNA immunization with pTc-sp7 specifically reacted with the native protein of 56 kDa previously reported, which is immunolocalized in the tegument of T. crassiceps cysticerci. The 56-kDa antigen is also present in Taenia solium oncospheres, cysticerci, and adult tissue. The protection induced in DNA-immunized mice and the observation that the injected plasmid remains as an episomic form within muscle cells, encouraged us to continue testing this procedure to prevent T. solium cysticercosis.
Rosas
G
G
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF, México.
Cruz-Revilla
C
C
Fragoso
G
G
López-Casillas
F
F
Pérez
A
A
Bonilla
M A
MA
Rosales
R
R
Sciutto
E
E
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000235:genetics; D002460:Cell Line; D003551:Cysticercosis / Q000276:immunology / Q000517:prevention & control*; D003552:Cysticercus / Q000235:genetics / Q000276:immunology*; D018076:DNA, Complementary / Q000032:analysis / Q000276:immunology; D018509:DNA, Helminth / Q000032:analysis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D015801:Helminth Proteins / Q000235:genetics; D007114:Immunization / Q000379:methods*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000737:chemistry / Q000166:cytology; D010957:Plasmids / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D014162:Transfection
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D011994:Recombinant Proteins