The evaluation of ciliary function: electron versus light microscopy.
Diagnosing Primary Ciliary Dyskinesia can often be difficult. Physical findings suggest the disease, but definitive diagnosis should be made with a ciliary biopsy. Twenty biopsies were obtained from 16 patients and all underwent both light and electron microscopic examination. In 8/20 (40%) there was a discrepancy between the different imaging techniques. Therefore, light microscopy should be used to assess adequacy of biopsy and motion of the cilia along with electron microscopy to examine ultrastructure.
Kupferberg
S B
SB
Division of Otolaryngology, Medical College of Georgia, Augusta, USA.
Bent
J P
JP
Porubsky
E S
ES
D001706:Biopsy; D001991:Bronchitis / Q000473:pathology / Q000503:physiopathology; D002908:Chronic Disease; D002923:Cilia / Q000502:physiology* / Q000648:ultrastructure; D002925:Ciliary Motility Disorders / Q000473:pathology / Q000503:physiopathology*; D004398:Dyneins / Q000032:analysis; D005069:Evaluation Studies as Topic; D006801:Humans; D008853:Microscopy; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D009068:Movement; D009092:Mucous Membrane / Q000473:pathology; D009297:Nasal Mucosa / Q000473:pathology; D010033:Otitis Media / Q000473:pathology / Q000503:physiopathology; D010256:Paranasal Sinuses / Q000473:pathology; D011014:Pneumonia / Q000473:pathology / Q000503:physiopathology; D012189:Retrospective Studies; D012852:Sinusitis / Q000473:pathology / Q000503:physiopathology; D014132:Trachea / Q000473:pathology
D004398:Dyneins