Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.
A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). N difference was found concerning the inhibition rate.
Jaulhac
B
B
Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur et Hopitaux Universitaires de Strasbourg, France. jaul-ibs@tpgnet.net
Reyrolle
M
M
Sodahlon
Y K
YK
Jarraud
S
S
Kubina
M
M
Monteil
H
H
Piémont
Y
Y
Etienne
J
J
D001431:Bacteriological Techniques; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D019286:Endopeptidase K; D006801:Humans; D016952:Legionella pneumophila / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011137:Polystyrenes; D011145:Polyvinyls; D011446:Prospective Studies; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial; D011137:Polystyrenes; D011145:Polyvinyls; C006960:chelex; D019286:Endopeptidase K