title: Improved wool production in transgenic sheep expressing insulin-like growth factor 1.
abstract: Transgenic sheep were produced by pronuclear microinjection with a mouse ultra-high-sulfur keratin promoter linked to an ovine insulin-like growth factor 1 (IGF1) cDNA. Five transgenic lambs resulted from the microinjection of 591 embryos; one male and one female showed IGF1 expression in the skin. A progeny test of the ram was carried out by matings to 43 non-transgenic ewes. Of 85 lambs born, 43 (50.6%) were transgenic. At yearling shearing (approximately 14 months of age), clean fleece weight was on average 6.2% greater in transgenic animals than in their non-transgenic half-sibs, with a greater effect in males (9.2%) than females (3.4%). Transgenics showed a small but significant increase in bulk, but male transgenics had a lower staple strength than female transgenics and non-transgenics which did not differ significantly. There were no significant differences in fiber diameter, medullation, and hogget body weight. To our knowledge this is the first reported improvement in a production trait by genetic engineering of a farm animal without adverse effects on health or reproduction.
authors.affiliation: Centre for Molecular Biology, Lincoln University, Canterbury, New Zealand.
authors.forename: S
authors.initials: S
authors.lastname: Damak
authors.forename: H
authors.initials: H
authors.lastname: Su
authors.forename: N P
authors.initials: NP
authors.lastname: Jay
authors.forename: D W
authors.initials: DW
authors.lastname: Bullock
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D005260:Female; D015870:Gene Expression; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D007633:Keratins / Q000235:genetics*; D008297:Male; D051379:Mice; D008845:Microinjections; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D012756:Sheep / Q000235:genetics*; D014935:Wool / Q000254:growth & development*
chemical_list: D007334:Insulin-Like Growth Factor I; D007633:Keratins