[Analysis of B-3 desamido insulin in human insulin preparations by HPLC].
The content of A-21 desamido insulin (A-21 DI) and B-3 desamido insulin (B-3 DI) in human insulin preparations was measured by new RP-HPLC method using a neutral eluent (pH 6.5). Sometimes the content of B-3 DI in human insulin preparations was about two times larger than that of A-21 DI. In particular, in the case of neutral insulin injection, the content of B-3 DI has increased remarkably as the increase of total desamido content. The content of B-3 DI obtained by the new RP-HPLC method will open the new aspect of the impurity test for insulin preparations.
Yomota
C
C
yomota@nihs.go.jp
Tagashira
Y
Y
Miyazaki
T
T
Okada
S
S
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007328:Insulin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000592:standards
D007328:Insulin; C018037:insulin, desamido (A21)-; C099825:insulin, desamido (B-3)