Cloning of the V-ATPase B subunit cDNA from Culex quinquefasciatus and expression of the B and C subunits in mosquitoes.
The V-ATPase B subunit cDNA isolated from the midgut and Malpighian tubules of Culex quinquefasciatus larvae has a 1476 bp ORF encoding a 492 amino acids protein with a predicted mass of 54.8 kDa. Northern blot analysis reveals the presence of 1.8 and 4.2 kb transcripts in larvae and a 3.0 kb transcript in pupae. A single 57 kDa protein band is detected in immunoblot analysis of protein extracts from C. quinquefasciatus and A. aegypti larvae. Using antibodies to the B subunits we demonstrate high-level expression of these subunits in ion-transporting cells of caeca and anterior midgut, and in Malpighian tubules and rectum. High V-ATPase expression was also observed in the larval salivary glands, central nervous system neurophile, the thoracic endocrine complex and in imaginal discs.
Filippova
M
M
Department of Entomology, University of California, Riverside 92521, USA.
Ross
L S
LS
Gill
S S
SS
D000330:Aedes; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D003001:Cloning, Molecular; D003465:Culex / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary; D015870:Gene Expression; D008969:Molecular Sequence Data; D006180:Proton-Translocating ATPases / Q000235:genetics*; D011817:Rabbits; D025262:Vacuolar Proton-Translocating ATPases
D018076:DNA, Complementary; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases