Artificial imitation of glutathione peroxidase with 6-selenium-bridged beta-cyclodextrin.
On the basis of cyclodextrin, 6-selenium bridged beta-cyclodextrin (6-beta-CD-Se-Se-beta-CD, known as 6-SeCD) was synthesized by the selective tosylation of beta-cyclodextrin and nucleophilic displacement by sodium hydroselenide to imitate glutathione peroxidase (GPX). The GPX activity of diselenide 6-SeCD is 4.3 times that of PZ51. The structure of the mimic 6-SeCD was characterized by means of laser mass spectroscopy, elemental analysis, IR and 1H NMR. The selenium content and its valence in 6-SeCD were determined by means of X-ray photoelectron spectra. Kinetics of the mimic showed that its enzymatic behavior was similar to that of native GPX.
Liu
J Q
JQ
The National Laboratory of Enzyme Engineering, Jilin University, Changchun, 130023, P.R. China.
Gao
S J
SJ
Luo
G M
GM
Yan
G L
GL
Shen
J C
JC
D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000378:metabolism; D003505:Cyclodextrins / Q000737:chemistry* / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D008956:Models, Chemical; D015394:Molecular Structure; D012643:Selenium / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D047392:beta-Cyclodextrins
D001952:Bridged-Ring Compounds; D003505:Cyclodextrins; D047392:beta-Cyclodextrins; D005979:Glutathione Peroxidase; D012643:Selenium; C031215:betadex