[Change in regulation of catabolite-dependent lux-operon, cloned in a recombinant plasmid, under the effect of environmental factors].
Expression of the lux-genes cloned on the recombinant plasmid pPHL7 (AprLux+) in Escherichia coli z905 cells was studied in various environments, including model aquatic ecosystems. Expression of the lux-genes strongly depended on the nutritional status of the medium. In particular, the cultivation of cells in nutrient-rich medium favored the maintenance of the initial level of expression of the lux-operon, whereas nutrient limitation induced recombinant cell variants with an impaired control of the catabolite-dependent lux-operon. On the other hand, long-term laboratory cultivation of recombinant strain in nutrient-deficient media or its long-term life in model aquatic ecosystems led to the accumulation of cells with a stringent control on the cloned lux-genes in the bacterial population. The presence of the selective factor (ampicillin) in the medium had no significant effect on the expression of the lux-operon.
Maksimova
E E
EE
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia.
Popova
L Iu
LIu
Shpagina
V V
VV
Beliavskaia
V A
VA
Pechurkin
N S
NS
D003001:Cloning, Molecular; D003470:Culture Media; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008163:Luminescent Measurements; D009876:Operon; D010957:Plasmids; D014871:Water Microbiology
D003470:Culture Media; D004274:DNA, Recombinant
Izmenenie reguliatsii katabolitzavisimogo lux-operona, klonirovannogo v rekominantnoĭ plazmide, pod vozdeĭstviem faktorov vneshneĭ sredy.