GAC1, a new member of the leucine-rich repeat superfamily on chromosome band 1q32.1, is amplified and overexpressed in malignant gliomas.
We have used two-dimensional electrophoresis of enzyme-digested genomic DNA to identify a novel gene GAC1, which maps at 1q32.1 and which is overexpressed in malignant gliomas in which it is amplified. GAC1 encodes a protein which belongs to the leucine-rich repeat superfamily. Amplification and overexpression of GAC1 was demonstrated in two of eight tumors where amplifications were previously evidenced by comparative genomic hybridization (one glioblastoma multiforme and one anaplastic astrocytoma), and in one of eight unselected glioblastomas multiforme. GAC1 exhibits sequence homology with other proteins which function as cell-adhesion molecules or as signal transduction receptor and is a likely candidate for the target gene in the 1q32.1 amplicon in malignant gliomas.
Almeida
A
A
Institut Curie-CNRS UMR 147, Paris, France.
Zhu
X X
XX
Vogt
N
N
Tyagi
R
R
Muleris
M
M
Dutrillaux
A M
AM
Dutrillaux
B
B
Ross
D
D
Malfoy
B
B
Hanash
S
S
D000595:Amino Acid Sequence; D001254:Astrocytoma / Q000235:genetics; D015816:Cell Adhesion Molecules, Neuronal; D002878:Chromosomes, Human, Pair 1; D015252:Deoxyribonucleases, Type II Site-Specific; D005784:Gene Amplification; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000235:genetics; D005910:Glioma / Q000235:genetics*; D006801:Humans; D007930:Leucine; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000737:chemistry / Q000235:genetics*; D009837:Oligodendroglioma / Q000235:genetics; D012083:Renin / Q000235:genetics; D014158:Transcription, Genetic
D015816:Cell Adhesion Molecules, Neuronal; C113373:LRRN2 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D015252:Deoxyribonucleases, Type II Site-Specific; C024405:GATC-specific type II deoxyribonucleases; C038565:GCGGCCGC-specific type II deoxyribonucleases; D012083:Renin; D007930:Leucine