Molecular cloning of a novel GTP-binding protein induced in fish cells by rhabdovirus infection.
We have cloned and sequenced a cDNA encoding GTP-binding protein from a fish cell, CHSE-214. The clone was 1493 bp long and contained an open reading frame encoding 364 amino acids. It has the five sequence motifs G1-G5 that are conserved in all GTP-binding proteins. Its amino acid sequences are strikingly different from those of the well-characterized G-proteins. However, sequences closely related to this protein are found in various kinds of species including human, Arabidopsis, Drosophila and archaebacteria, suggesting a novel subfamily within the superfamily of the GTP-binding proteins. Northern analysis indicates that this gene is constitutively expressed at a low level in normal cells but is induced by fish rhabdovirus infection at about 24 h post infection and disappears thereafter. Based on these observations, we propose that this protein represents an evolutionarily conserved novel subfamily of GTP-binding proteins which may play an important role in fish rhabdovirus infection.
Lee
E H
EH
Department of Molecular Biology, Pusan National University, South Korea.
Kim
H J
HJ
Park
J J
JJ
Choi
J Y
JY
Cho
W J
WJ
Cha
S J
SJ
Moon
C H
CH
Park
J M
JM
Yoon
W J
WJ
Lee
B J
BJ
Lee
D H
DH
Kang
H S
HS
Yoo
M A
MA
Kim
H D
HD
Park
J W
JW
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005393:Fish Diseases / Q000821:virology*; D019204:GTP-Binding Proteins / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D018353:Rhabdoviridae Infections / Q000662:veterinary*; D012474:Salmon / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D019204:GTP-Binding Proteins