title: Automated free-solution isotachophoresis: instrumentation and fractionation of human serum proteins.
abstract: An automated free-solution isotachophoresis system (FS-ITP) for preparative fractionation of biopolymers is described, operated in a batch mode. The dimension of the separation chamber allows an up to 1200-fold higher sample load compared to separation in capillaries of 180 microm inner diameter as used in analytical capillary isotachophoresis (C-ITP). The preparative capacity of the system is within the milligram range. The method is fully compatible with analytical C-ITP, which is essential for preparative-scale isotachophoresis with regard to optimization of electrolyte systems and the search for suitable spacers. As a model application the fractionation of human serum proteins is reported. The collected fractions were analyzed by C-ITP and agarose gel electrophoresis.
authors.affiliation: Institute for Clinical Chemistry and Laboratory Medicine, University of Regensburg, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Böttcher
authors.forename: C
authors.initials: C
authors.lastname: Möllers
authors.forename: K J
authors.initials: KJ
authors.lastname: Lackner
authors.forename: G
authors.initials: G
authors.lastname: Schmitz
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods*; D006801:Humans; D012996:Solutions
chemical_list: D001798:Blood Proteins; D012996:Solutions