Isolation and characterization of an endogenous inhibitor of phospholipase A2 from Indian cobra (Naja naja naja) venom.
A PLA2-inhibitor has been purified from Indian cobra (Naja naja naja) venom by the combination of ion-exchange and gel-filtration chromatography. The inhibitor, NN-I3 was a peptide with mol.wt 6500 and has a fluorescence emission maxima ca 340 nm. NN-I3 specifically inhibited the enzyme activity of the three acidic PLA2 from the same venom. The inhibition of NN-I2d-PLA2 and NN-I2c-PLA2 by NN-I3 was of mixed type and NN-I2c-PLA2 was of uncompetitive type. Neither the inhibitor nor the individual mixtures of acidic PLA2 with the inhibitor (1:1 w/w or 1:2 mol:mol) were lethal to mice when injected intraperitoneally in doses up to 10 mg kg(-1) body weight.
Rudrammaji
L M
LM
Department of Studies in Biochemistry, University of Mysore, Manasagangotri, India.
Gowda
T V
TV
D000818:Animals; D004546:Elapid Venoms / Q000737:chemistry* / Q000201:enzymology / Q000494:pharmacology; D017815:Elapidae; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology*; D007194:India; D010741:Phospholipases A / Q000037:antagonists & inhibitors*; D054467:Phospholipases A2; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology
D004546:Elapid Venoms; D004791:Enzyme Inhibitors; D011506:Proteins; D010741:Phospholipases A; D054467:Phospholipases A2