title: Effects of rhinovirus infection on hydrogen peroxide- induced alterations of barrier function in the cultured human tracheal epithelium.
abstract: To investigate whether rhinovirus infection impairs epithelial barrier functions, human rhinovirus 14 (HRV-14) was infected to primary cultures of human tracheal epithelial cells and experiments were performed on Day 2 after HRV-14 infection. Hydrogen peroxide (H2O2; 3 x 10(-)4 M) increased electrical conductance (G) across the epithelial cell sheet measured with Ussing's chamber methods. Exposure of the epithelial cells to HRV-14 had no effect on H2O2-induced increases in G and [3H]mannitol flux through the cultured epithelium in the control condition, but it markedly potentiated H2O2- induced increases in both parameters in IL-1beta (100 U/ml) pretreated condition. However, pretreatment with TNF-alpha (100 U/ml) was without effect. IL-1beta enhanced the intercellular adhesion molecule-1 (ICAM-1) expression assessed by immunohistochemical analysis and susceptibility of epithelial cells to HRV-14 infection. An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-1beta-pretreated epithelial cells with HRV-14 infection. These results suggest that rhinovirus infection may reduce barrier functions in the airway epithelium in association with upregulation of ICAM-1 expression.
authors.affiliation: Department of Geriatric Medicine, Tohoku University School of Medicine, Sendai 980, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Ohrui
authors.forename: M
authors.initials: M
authors.lastname: Yamaya
authors.forename: K
authors.initials: K
authors.lastname: Sekizawa
authors.forename: N
authors.initials: N
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Terajima
authors.forename: S
authors.initials: S
authors.lastname: Okinaga
authors.forename: H
authors.initials: H
authors.lastname: Sasaki
mesh_terms: D000911:Antibodies, Monoclonal; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D016877:Oxidants / Q000494:pharmacology*; D010850:Picornaviridae Infections / Q000473:pathology / Q000503:physiopathology*; D012229:Rhinovirus; D014132:Trachea / Q000166:cytology*
chemical_list: D000911:Antibodies, Monoclonal; D016877:Oxidants; D018799:Intercellular Adhesion Molecule-1; D006861:Hydrogen Peroxide