title: Determination of ropivacaine and [2H3]ropivacaine in biological samples by gas chromatography with nitrogen-phosphorus detection or mass spectrometry.
abstract: Bioanalytical methods for determining the total concentration of the new local anaesthetic drug ropivacaine in blood plasma, urine and tissues are presented. Ropivacaine is a drug mainly used in connection with surgery and for post-operative pain relief. The biological samples were prepared using liquid-liquid extraction and analysed using capillary gas chromatography with nitrogen-phosphorus detection or mass spectrometry. The methods are highly selective and reliable with a between-day precision, given as the relative standard deviation, generally below 6%. More than 20000 samples have been analysed using the methods described.
authors.affiliation: Concept Division, Bioanalytical Chemistry, Astra Pain Control, Södertälje, Sweden.
authors.forename: M
authors.initials: M
authors.lastname: Engman
authors.forename: P
authors.initials: P
authors.lastname: Neidenström
authors.forename: C
authors.initials: C
authors.lastname: Norsten-Höög
authors.forename: S J
authors.initials: SJ
authors.lastname: Wiklund
authors.forename: U
authors.initials: U
authors.lastname: Bondesson
authors.forename: T
authors.initials: T
authors.lastname: Arvidsson
mesh_terms: D000577:Amides / Q000032:analysis*; D000779:Anesthetics, Local / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D000077212:Ropivacaine
chemical_list: D000577:Amides; D000779:Anesthetics, Local; D000077212:Ropivacaine