Morphologic, biometric, and isoenzyme characterization of Trichuris suis.
Trichuris suis isolates were collected from the cecum of Sus scrofa domestica (pig) and S. s. scrofa (wild boar). Morphology and biometry studies were carried out. Morphology studies showed the existence of typical caudal papillae in males of T. suis from wild boars, but no other difference was observed in the biometric parameters (total length, esophageal length, posterior-portion body length, and spicular length) of T. suis isolated from either host. Individual extracts were subjected to malate dehydrogenase (MDH), malic enzyme (ME), glucose 6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) isoenzyme analysis following starch-gel electrophoresis, and the isoenzyme patterns were compared with those obtained from other species of trichurids. MDH, ME, G6PD, LDH, and SOD isoenzyme patterns were identical for T. suis from both hosts. MDH isoenzyme patterns were characterized by the presence of one cathodic isoenzyme. ME, G6PD, and LDH isoenzyme patterns indicated the presence of three phenotypes, whereas the SOD isoenzyme pattern showed only one phenotype characterized by the existence of two (anodic and cathodic) bands. Different LDH and SOD isoenzyme patterns observed for T. suis, T. ovis, and T. skrjabini confirm once more that isoenzyme patterns have potential as a diagnostic tool for differentiation of different species of Trichuris.
Oliveros
R
R
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, Spain.
Cutillas
C
C
Arias
P
P
Guevara
D
D
D000818:Animals; D004592:Electrophoresis, Starch Gel; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000737:chemistry / Q000235:genetics; D007527:Isoenzymes / Q000737:chemistry* / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics; D008291:Malate Dehydrogenase / Q000737:chemistry / Q000235:genetics; D008297:Male; D010641:Phenotype; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics; D013552:Swine / Q000469:parasitology; D014258:Trichuris / Q000033:anatomy & histology* / Q000201:enzymology* / Q000235:genetics
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase