title: Induction of prostate tumor-specific CD8+ cytotoxic T-lymphocytes in vitro using antigen-presenting cells pulsed with prostatic acid phosphatase peptide.
abstract: BACKGROUND
  Most strategies in cancer immunotherapy are aimed at the induction of a strong cellular immune response against the tumor. Particularly, CD8+ T lymphocytes have been proven in multiple animal models to be critical for the eradication of solid tumors.
  
  
  METHODS
  We used a population of peripheral blood-derived antigen-presenting cells (APC), containing dendritic cells (DC), to generate prostate tumor-specific CD8+ T cells. Selected peptides from prostatic acid phosphatase (PAP), a prostate tissue-specific antigen, were shown to bind HLA-A2. A high-affinity peptide was used to generate peptide-specific CD8+ cytolytic T lymphocytes (CTL) from the peripheral blood of healthy donors.
  
  
  RESULTS
  The obtained PAP-peptide-specific CTL lysed peptide-coated target cells, vaccinia-infected target cells, and HLA-A2-positive prostate-tumor cells in vitro in an antigen-specific manner.
  
  
  CONCLUSIONS
  Our results indicate that CTL precursors to the PAP gene product exist and could be potentially recruited to elicit an antitumor response. Thus, PAP is a suitable antigen for inclusion in prostate cancer vaccines.
authors.affiliation: Dendreon Corporation, Mountain View, California 94043, USA. mvpeshwa@dendreon.com
authors.forename: M V
authors.initials: MV
authors.lastname: Peshwa
authors.forename: J D
authors.initials: JD
authors.lastname: Shi
authors.forename: C
authors.initials: C
authors.lastname: Ruegg
authors.forename: R
authors.initials: R
authors.lastname: Laus
authors.forename: W C
authors.initials: WC
authors.lastname: van Schooten
mesh_terms: D000135:Acid Phosphatase / Q000737:chemistry / Q000276:immunology*; D000938:Antigen-Presenting Cells / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D003713:Dendritic Cells / Q000276:immunology; D015789:HLA-A2 Antigen / Q000378:metabolism; D006801:Humans; D008297:Male; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology*; D011467:Prostate / Q000201:enzymology*; D011471:Prostatic Neoplasms / Q000276:immunology*
chemical_list: D015789:HLA-A2 Antigen; D010446:Peptide Fragments; D000135:Acid Phosphatase