Evaluation of biological responses to polymeric biomaterials by RT-PCR analysis III: study of HSP 70, 90 and 47 mRNA expression.
In this study, in order to analyze how cells recognize biomaterials, mRNA was evaluated on various substrates as shown in the attached HeLa S3 cells. The expressed Heat-Shock Protein (HSP) 70A, 70B, 90 and 47 mRNA were isolated and detected using the RT-PCR method. As a result, 70B, 90 and 47 mRNA expressions varied with differences in the hydrophilicity hydrophobicity of the substrates. HSP mRNAs outcome was induced significantly in the HeLa S3 cells that had adhered onto the hydrophilic surfaces. On the other hand, in the cells that had adhered to the hydrophobic surfaces, HSP mRNAs expressions were low. In the non-adhered HeLa S3 cells the tendency found in HSP 70B and HSP 47 mRNA's expressions was the same as that found in adhered cells, while there were no significant differences in the HSP 90 mRNA expression among either of the samples. We have concluded that HSP mRNAs expression is an important marker in the study of cell-polymer interactions.
Kato
S
S
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Japan.
Akagi
T
T
Sugimura
K
K
Kishida
A
A
Akashi
M
M
D001672:Biocompatible Materials / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D005069:Evaluation Studies as Topic; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011108:Polymers / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
D001672:Biocompatible Materials; D006360:Heat-Shock Proteins; D011108:Polymers; D012333:RNA, Messenger