BDNF and TrkB expression in intrastriatal ventral mesencephalic grafts in a rat model of Parkinson's disease.
We investigated the expression of BDNF and its high affinity receptor trkB in fetal dopaminergic grafts in a rat model of Parkinson's disease. Grafts were allowed to differentiate for 7, 14, 28, or 56 days, respectively and were analyzed immunocytochemically thereafter with antibodies directed against tyrosine hydroxylase, BDNF and trkB. At all time points investigated, grafts contained tyrosine hydroxylase immunoreactive neurons. Immature grafts (7 days) displayed no immunoreactivity for BDNF which was restricted to glial cells at the graft-host interface. After longer differentiation periods BDNF-immunoreactivity was detectable in neurons and astrocytes within the grafts. No trkB immunoreactivity was found in immature grafts but a strong signal for trkB emerged in grafted neurons older than 14 days whereas glial cells remained unlabeled at all time points investigated. Expression of BDNF and trkB in grafted neurons and of BDNF in surrounding glial cells suggests an autocrine or paracrine action of BDNF on dopaminergic neurons possibly mediated by activated glia.
Sautter
J
J
Institute of Physiology, University of Munich, Federal Republic of Germany.
Sabel
M
M
Sommer
C
C
Strecker
S
S
Weidner
N
N
Oertel
W H
WH
Kiessling
M
M
D000818:Animals; D016380:Brain Tissue Transplantation / Q000502:physiology*; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D005260:Female; D006892:Hydroxydopamines; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000637:transplantation*; D017072:Neostriatum / Q000378:metabolism*; D010302:Parkinson Disease, Secondary / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000096:biosynthesis*; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000096:biosynthesis*; D013565:Sympatholytics; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D019208:Brain-Derived Neurotrophic Factor; D006892:Hydroxydopamines; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D013565:Sympatholytics; D014446:Tyrosine 3-Monooxygenase; D020794:Receptor Protein-Tyrosine Kinases