Down-regulation of tumor necrosis factor-alpha, moderate reduction of interleukin-1beta, but not interleukin-6 or interleukin-10, by glucan immunomodulators curdlan sulfate and lentinan.
The effects of glucan-based immunomodulators curdlan sulfate (CRDS) and lentinan on cytokine production stimulated by lipopolysaccharide (LPS) in bacillus Calmette-Guerin (BCG)-primed mice were investigated. Pretreatment with CRDS or lentinan before LPS administration induced a striking inhibition of up to 89% of circulating tumor necrosis factor-alpha (TNF), a moderate reduction of 25% of interleukin (IL)-1beta, no significant differences in IL-6 or IL-10 levels, and a marked depression of chemiluminescence activity. Animals receiving CRDS prior to infection with alpha-hemolysin positive Escherichia coli inhibited measurable TNF production by 63%. The ability of CRDS and lentinan to significantly reduce the TNF production in vivo indicates the potential of glucans in possible therapeutic strategies that are based on down-regulation of TNF.
Masihi
K N
KN
Robert Koch Institute, Berlin, Germany.
Madaj
K
K
Hintelmann
H
H
Gast
G
G
Kaneko
Y
Y
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D015536:Down-Regulation / Q000187:drug effects; D004927:Escherichia coli Infections / Q000276:immunology; D005260:Female; D005936:Glucans / Q000494:pharmacology*; D018836:Inflammation Mediators / Q000097:blood; D007375:Interleukin-1 / Q000096:biosynthesis / Q000097:blood*; D016753:Interleukin-10 / Q000096:biosynthesis / Q000097:blood; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood; D007912:Lentinan / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008163:Luminescent Measurements; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*; D047071:beta-Glucans
D000276:Adjuvants, Immunologic; D005936:Glucans; D018836:Inflammation Mediators; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D047071:beta-Glucans; D016753:Interleukin-10; D007912:Lentinan; C063130:curdlan sulfate