Colocalization of Bcl-2 and 4-hydroxynonenal modified proteins in microglial cells and neurons of rat brain following transient focal ischemia.
Bcl-2 has a role in suppressing the production of reactive oxygen species and lipid peroxidation. To explore the in situ localization of 4-hydroxy-2-nonenal (HNE)-modified proteins and the Bcl-2 oncoprotein, we used double immunofluorescence labeling and confocal imaging in the rat brain after 3 h of middle cerebral artery (MCA) occlusion followed by reperfusion. Immunoreactivity for HNE or Bcl-2 was not detected at 1 h, but appeared in some intact neurons in the boundary between the infarcted and non-infarcted zones at 12 h. At 48 h, HNE-positive microglia were colocalized with Bcl-2 in the infarcted area and the boundary zone. Bcl-2 may play an important role in the antioxidant system promoting survival of the neurons and activated microglia following reperfusion injury.
Urabe
T
T
Department of Neurology, Juntendo University School of Medicine, Bunkyo, Tokyo, Japan. t-urabe@med.juntedo.ac.jp
Hattori
N
N
Yoshikawa
M
M
Yoshino
H
H
Uchida
K
K
Mizuno
Y
Y
D000447:Aldehydes / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation; D008297:Male; D017628:Microglia / Q000737:chemistry*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry; D009474:Neurons / Q000737:chemistry*; D018384:Oxidative Stress; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D000447:Aldehydes; D009419:Nerve Tissue Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C027576:4-hydroxy-2-nonenal