Stimulation of interleukin-6 production in human dental pulp cells by peptidoglycans from Lactobacillus casei.
Interleukin-6 (IL-6), which is a multifunctional cytokine, has an important role in acute and chronic inflammation. The peptidoglycan (PG) was purified from Lactobacillus casei, which was a Gram-positive bacteria frequently isolated from deep carious lesions and suspected to be a pathogen of pulpitis. In this study, the effects of PG on the production of IL-6 in human dental pulp cells were examined. PG stimulated IL-6 production in a time- and dose-dependent manner. Reverse transcriptase-polymerase chain reaction experiments showed that the increase was dependent on the enhancement of IL-6 mRNA levels. These findings suggest that Gram-positive bacteria, such as L. casei, from carious lesions, might be involved in developing pulpitis through the stimulation of IL-6 production.
Matsushima
K
K
Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Ohbayashi
E
E
Takeuchi
H
H
Hosoya
S
S
Abiko
Y
Y
Yamazaki
M
M
D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D007780:Lacticaseibacillus casei / Q000737:chemistry / Q000378:metabolism*; D010457:Peptidoglycan / Q000378:metabolism / Q000494:pharmacology*; D011671:Pulpitis / Q000209:etiology; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation
D015850:Interleukin-6; D010457:Peptidoglycan; D012333:RNA, Messenger