[The staining of the central neurons in the pteropod mollusk Clione limacina with fluorescein-labelled alpha-bungarotoxin].
FITC-labelled alpha-bungarotoxin (alpha BT[FITC]) was used to identify acetylcholine (ACh)-sensitive neurons in the central nervous system of pteropod mollusc Clione limacina. Two small symmetrical neurons were stained with alpha BT[FITC] on the dorsal surface of the cerebral ganglia. The staining was highly specific as it was completely blocked by preincubation in alpha-bungarotoxin [alpha BT] at very low concentration (10(-9) M). Nicotinic agonists and antagonists efficiently prevented alpha BT[FITC] staining while muscarinic agonists and antagonists had no effect. These findings confirm the suggestion that alpha BT[FITC] binds to nicotinic ACh receptors. The possible role is discussed of alpha BT[FITC]-stained cells in the complex of hunting behavior of Clione.
Popova
L B
LB
Belozersky Institute of Physico-Chemical Biology, Lomonosov State University, Moscow.
Panchin
Iu V
IuV
D000109:Acetylcholine / Q000378:metabolism; D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology*; D004396:Coloring Agents / Q000494:pharmacology*; D004347:Drug Interactions; D019793:Fluorescein / Q000494:pharmacology*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008974:Mollusca / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000187:drug effects / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D002038:Bungarotoxins; D004396:Coloring Agents; D011950:Receptors, Cholinergic; D000109:Acetylcholine; D019793:Fluorescein
Okraska tsentral'nykh neĭronov krylonogogo molliuska Clione limacina al'fa-bungarotoksinom, mechennym fliuorestseinom.