title: Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells.
abstract: Truncation of human chromosomes at desired sites by homologous recombination techniques enables functional and structural analyses of human chromosomes and development of human artificial chromosomes. However, this targeted truncation has been inefficient. We describe here an efficient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate. The human chromosome 22 was transferred into DT40 cells, where human telomeric repeat (TTAGGG)n was targeted to the LIF locus on the chromosome. Molecular and cytogenetic analyses showed that the predicted truncation at the LIF locus occurred in all of the targeted clones.
authors.affiliation: Central Laboratories for Key Technology, Kirin Brewery Company Ltd, 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroiwa
authors.forename: T
authors.initials: T
authors.lastname: Shinohara
authors.forename: T
authors.initials: T
authors.lastname: Notsu
authors.forename: K
authors.initials: K
authors.lastname: Tomizuka
authors.forename: H
authors.initials: H
authors.lastname: Yoshida
authors.forename: S
authors.initials: S
authors.lastname: Takeda
authors.forename: M
authors.initials: M
authors.lastname: Oshimura
authors.forename: I
authors.initials: I
authors.lastname: Ishida
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002645:Chickens; D002892:Chromosomes, Human, Pair 22; D017931:DNA Primers; D006801:Humans; D011995:Recombination, Genetic; D016615:Telomere
chemical_list: D017931:DNA Primers