Detection of point mutations in rpoB gene of rifampin-resistant Rickettsia typhi.
The rpoB gene of rifampin-resistant Rickettsia typhi (Rif mutant) and wild-type R. typhi were sequenced and compared. The Rif mutant rpoB had three nucleotide substitutions, which resulted in amino acid changes at residues 151, 201, and 271 and may be the basis for the rifampin resistance.
Troyer
J M
JM
Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore 21201, USA.
Radulovic
S
S
Andersson
S G
SG
Azad
A F
AF
D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017354:Point Mutation; D012286:Rickettsia typhi / Q000187:drug effects / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology*
D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit; D012293:Rifampin