Genomic organization and cloning of the human homologue of murine Sipa-1.
Murine Sipa-1 (signal-induced proliferation associated protein) is a mitogen induced GTPase activating protein (GAP). While mapping candidate genes for multiple endocrine neoplasia type 1 (MEN1) at 11q13, we cloned the human homologue of Sipa-1. Herein, we report the complete cDNA sequence, expression, and genomic organization of SIPA-1. SIPA-1 consists of 16 exons with highly conserved exon-intron boundaries. The predicted SIPA-1 protein is highly homologous to the mouse protein, particularly in the region of the GAP-related domain at the amino terminus and the leucine zipper at the carboxy terminus. It is widely expressed, including in fetal tissues, but is most highly expressed in lymphoid organs. During the course of cloning SIPA-1, the MEN1 gene was identified, thus excluding human SIPA-1 as a candidate for this disease.
Ebrahimi
S
S
UCLA School of Medicine, West Los Angeles VA Medical Center Department of Surgery Core Molecular Biology Unit, 10833 Le, Conte Ave. 72-215 CHS, Los Angeles, CA 90095-6904, USA.
Wang
E
E
Udar
N
N
Arnold
E
E
Burbee
D
D
Small
K
K
Sawicki
M P
MP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005091:Exons; D005260:Female; D020690:GTPase-Activating Proteins; D015870:Gene Expression; D006801:Humans; D007438:Introns; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014018:Tissue Distribution
D018076:DNA, Complementary; D020690:GTPase-Activating Proteins; D009687:Nuclear Proteins; D011506:Proteins; D012333:RNA, Messenger; C490403:SIPA1 protein, human; C091108:Sipa1 protein, mouse