title: Zero-crossing derivative spectrophotometry for the determination of haloperidol in presence of parabens.
abstract: First derivative spectrophotometry with a zero-crossing technique of measurement is used for the quantitative determination of haloperidol in the presence of methylparaben and propylparaben, which is added as antimicrobial preservatives in pharmaceuticals. This technique permits the quantification of haloperidol in the presence of parabens, with closely overlapping spectral bands, and without any separation step. Linear calibration graphs of first derivative values (at 255.2 nm for haloperidol) versus concentration (in the range 4.0-20.0 micrograms ml-1) were obtained with negligible intercepts. Relative standard deviation of 0.83% was obtained for intra-day precision and 1.86% for inter-day precision. The recovery of haloperidol in synthetic mixtures with parabens and pharmaceutical dosage forms is also reported.
authors.affiliation: Laboratoire National de Contrôle des Médicaments, Bab Saadoun, Tunis, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Ouanês
authors.forename: M
authors.initials: M
authors.lastname: Kallel
authors.forename: H
authors.initials: H
authors.lastname: Trabelsi
authors.forename: F
authors.initials: F
authors.lastname: Safta
authors.forename: K
authors.initials: K
authors.lastname: Bouzouita
mesh_terms: D002138:Calibration; D006220:Haloperidol / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D010226:Parabens / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D010226:Parabens; C015358:methylparaben; D006220:Haloperidol; C006068:propylparaben