Variation in the proregion structure of heparin-binding EGF-like growth factor precursors.
In a previous study, we have isolated and characterized cDNA encoding a novel 'short form' of heparin-binding EGF-like growth factor (SF HB-EGF) (Loukianov et al., 1997). In the present work, we have found that cDNA for SF HB-EGF and for full-length HB-EGF are each represented by two variants, which we refer to as L and P forms. The L form is the previously known form of HB-EGF cDNA and encodes a leucine in position 33. The P form described in this report, encodes a proline in codon 33. The L33P substitution is predicted to cause a significant alteration in the proregion structure of SF HB-EGF and HB-EGF.
Loukianov
E V
EV
Department of Biochemistry, Norwegian Radium Hospital, Oslo, Norway.
Loukianova
T
T
Wiedlocha
A
A
Olsnes
S
S
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004815:Epidermal Growth Factor / Q000737:chemistry* / Q000235:genetics*; D014644:Genetic Variation; D006367:HeLa Cells; D066257:Heparin-binding EGF-like Growth Factor; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011498:Protein Precursors / Q000737:chemistry* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014709:Vero Cells
D018076:DNA, Complementary; C587883:HBEGF protein, human; D066257:Heparin-binding EGF-like Growth Factor; D036341:Intercellular Signaling Peptides and Proteins; D011498:Protein Precursors; D012333:RNA, Messenger; D004815:Epidermal Growth Factor