title: Antisense inhibition of expression of cysteine proteinases does not affect Entamoeba histolytica cytopathic or haemolytic activity but inhibits phagocytosis.
abstract: Inhibition of most of the expression of the cysteine proteinases of Entamoeba histolytica strain HM-1:IMSS was successfully performed by transcription of ehcp5 antisense RNA using the promoter of ehg34, which encodes a L21 ribosomal protein of E. histolytica. We have generated a stable transfectant in which the overall level of cysteine proteinase activity is strongly reduced ( 90%). This transfectant has a normal growth rate in Diamond's TYI-S-33 medium, a cytopathic and haemolytic activity similar to the control HM-1:IMSS pEhAct-Neo transfectant but with a significantly lower phagocytic activity.
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Ankri
authors.forename: T
authors.initials: T
authors.lastname: Stolarsky
authors.forename: D
authors.initials: D
authors.lastname: Mirelman
mesh_terms: D000180:Acrylic Resins; D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D003470:Culture Media; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D004912:Erythrocytes / Q000378:metabolism; D018093:Gelatinases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D017125:Genes, Protozoan; D015801:Helminth Proteins; D006462:Hemolytic Plaque Technique; D006801:Humans; D010587:Phagocytosis; D016372:RNA, Antisense / Q000494:pharmacology*; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D000180:Acrylic Resins; D003470:Culture Media; D015801:Helminth Proteins; D016372:RNA, Antisense; C016680:polyacrylamide gels; D000426:Alcohol Dehydrogenase; D003546:Cysteine Endopeptidases; D018093:Gelatinases