title: Nucleotide sequence and chromosomal location of L-lactate dehydrogenase gene from Streptococcus pneumoniae.
abstract: We report here the 1244-bp sequence of a Streptococcus pneumoniae chromosomal fragment that contains the putative promoter and protein-coding region of the lactate dehydrogenase gene (ldh). The nucleotide sequence predicts a protein of 327 aa with a molecular weight of 35,202 daltons, after removal of the N-terminal methionine residue. The ldh gene is located on the ApaI fragment 1 and SmaI fragment 2 of the previously reported physical map of S. pneumoniae chromosome.
authors.affiliation: Laboratorio de Referencia de Neumococos, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda E-28220, Madrid, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Jado
authors.forename: A
authors.initials: A
authors.lastname: Fenoll
authors.forename: J
authors.initials: J
authors.lastname: Casal
authors.forename: A
authors.initials: A
authors.lastname: Pérez
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D017124:Conserved Sequence / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013296:Streptococcus pneumoniae / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D007770:L-Lactate Dehydrogenase