Rapid and simple chromatographic method for the determination of diazepam and its major metabolites in human plasma and urine.
A simple, rapid, sensitive and selective HPLC method has been developed for the analysis of diazepam (DZP) and its major metabolites, N-desmethyldiazepam (DMDZP), temazepam (TZP) and oxazepam (OZP), in plasma and urine, using clonazepam (CZP) as the internal standard and chloroform as the extracting solvent, with a 10 ng/ml limit of quantitation for the four assayed drugs, and an average (+/-S.D.) recovery of 87.7+/-6.46%, 92.9+/-5.31%, 91.4+/-4.01% and 91.7+/-2.68% for DZP, DMDZP, TZP and OZP, respectively (from plasma), and 89.6+/-2.26%, 90+/-4.24%, 87.45+/-0.64% and 94.50+/-0.71% for DZP, DMDZP, TZP and OZP, respectively (from urine). The method has also proved to be selective and reproducible.
Azzam
R M
RM
Pharmaceutical Research Unit, Royal Scientific Society, Amman, Jordan.
Notarianni
L J
LJ
Ali
H M
HM
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002998:Clonazepam / Q000737:chemistry; D003975:Diazepam / Q000097:blood* / Q000737:chemistry / Q000652:urine*; D006801:Humans; D003708:Nordazepam / Q000097:blood / Q000652:urine; D010076:Oxazepam / Q000097:blood / Q000652:urine; D012016:Reference Values; D013693:Temazepam / Q000097:blood / Q000652:urine
D002998:Clonazepam; D003708:Nordazepam; D010076:Oxazepam; D013693:Temazepam; D003975:Diazepam