[The effect of mitomycin C on activities of carbonic anhydrase and ATPase of the ciliary body epithelium in rabbit].
OBJECTIVE
Enzymohistochemical methods were performed to observe the effect of mitomycin C(MMC) on the activities of carbonic anhydrase (CA) and ATPase of the ciliary body epithelium in rabbits after using 0.2mg/ml mitomycin C during sclerectomy for the further study on the mechanism of the decrease of intraocular pressure postoperatively.


METHODS
0.2mg/ml mitomycin C was topically applicated during the sclerectomy in rabbit eyes. 7 days postoperatively, Hasson's method and plumbum nitrate's method were used to stain the CA and ATPase of the ciliary body epithelium. The grey value of the enzymes was quantitated by Image Analysor.


RESULTS
MMC inhibited the activities of CA and ATPase of the ciliary body epithelium underlying the site of MMC application, but the difference of enzymatic activities of the region 90 degree apart from the surgical site and that of surgical site in normal saline control group was not statistically obvious (P < 0.05).


CONCLUSIONS
MMC could inhibit the activities of CA and ATPase of ciliary body epithelium, thus leading to the decreased secretion of humor aqueous and the lowerdown of intraocular pressure even persistent hypotony.
Xia
X
X
Department of Ophthalmology, Xiangya Hospital, Hunan Medical University, Changsha, China.
Huang
P
P
Jiang
Y
Y
Zhou
J
J
Wang
C
C
Wu
Z
Z
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism*; D002924:Ciliary Body / Q000166:cytology / Q000201:enzymology*; D004848:Epithelium / Q000187:drug effects; D005260:Female; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D011817:Rabbits
D019384:Nucleic Acid Synthesis Inhibitors; D016685:Mitomycin; D000251:Adenosine Triphosphatases; D002256:Carbonic Anhydrases