[Usage of double immuno-enzyme labeled staining method in forensic pathology].
In this paper we report a kind of double immunoenzyme labelled staining method (single enzyme method). The principle of the method is that HPR will show blue-black color when it meet DAB-CoCL2/H2O2, while it will show brown color as it meet DAB/H2O2, two colors constract very remarkably. This method was used to detect the antigens of hearts and brains which obtained from forensic autopsy. The results showed that the method was sensitive, simple and economical. One of the advantages is that the stained sections can keep for a long time. Thus, is very practical for forensic pathology.
Zhou
W
W
Department of Forensic Pathology, Sun Yat-sen University of Medical Sciences.
Zhu
J
J
Xu
X
X
D001921:Brain / Q000473:pathology*; D005554:Forensic Medicine; D006801:Humans; D007124:Immunoenzyme Techniques; D009206:Myocardium / Q000473:pathology*