title: Mutation analysis of the c-mos proto-oncogene in human ovarian teratomas.
abstract: Female transgenic mice lacking a functional c-mos proto-oncogene develop ovarian teratomas, indicating that c-mos may behave as a tumour-suppressor gene for this type of tumour. We have analysed the entire coding region of the c-MOS gene in a series of human ovarian teratomas to determine whether there are any cancer-causing alterations. DNA from twenty teratomas was analysed by single-strand conformational analysis (SSCA) and heteroduplex analysis (HA) to screen for somatic and germline mutations. In nine of these tumours the entire gene was also sequenced. A previously reported polymorphism and a single new sequence variant were identified, neither of which we would predict to be disease-causing alterations. These results suggest that mutations in the coding region of the c-MOS gene do not play a significant role in the genesis of human ovarian teratomas.
authors.affiliation: CRC Human Cancer Genetics Research Group, Addenbrooke's Hospital, Cambridge, UK.
authors.forename: K A
authors.initials: KA
authors.lastname: de Foy
authors.forename: S A
authors.initials: SA
authors.lastname: Gayther
authors.forename: W H
authors.initials: WH
authors.lastname: Colledge
authors.forename: S
authors.initials: S
authors.lastname: Crockett
authors.forename: I V
authors.initials: IV
authors.lastname: Scott
authors.forename: M J
authors.initials: MJ
authors.lastname: Evans
authors.forename: B A
authors.initials: BA
authors.lastname: Ponder
mesh_terms: D004252:DNA Mutational Analysis; D005260:Female; D016258:Genes, mos; D006801:Humans; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D000090063:Proto-Oncogene Mas; D013724:Teratoma / Q000235:genetics*