[Expression of intermediate filament proteins in MDCK cells cultured in the three-dimensional gel in the presence of cytokine HGF/SF].
An immunomorphologic study was made of expression of intermediate filament proteins--keratin and vimentin--in the structures formed in branching tubulogenesis of Madin-Darby Canine Kidney epithelial cells (MDCK, clone 20), cultured in three-dimensional collagen gel in the presence of the "scatter factor" (HGF/SF). An improved method of parallel immunostaining of the whole branching structures and of their cryostat cross-sections was used. It was shown that both the discoid epithelial cells, forming walls of tubules with inner clear spaces, and the fibroblast-like cells invading the collagenic gel coexpressed keratin and vimentin. Thus, in the studied system of simplified morphogenesis in vitro, some conservatism of intermediate filament protein expression was revealed which does not respond to changes in cell shape or cell localization.
Moĭzhess
T G
TG
Institute of Carcinogenesis, Oncological Research Center, Russian, Academy of Medical Sciences, Moscow.
Chipysheva
T A
TA
Gel'shteĭn
V I
VI
D000818:Animals; D002460:Cell Line; D003470:Culture Media; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D007633:Keratins / Q000096:biosynthesis*; D014746:Vimentin / Q000096:biosynthesis*
D003470:Culture Media; D014746:Vimentin; D017228:Hepatocyte Growth Factor; D007633:Keratins
Ekspressiia belkov promezhutochnykh filamentov v kletkakh MDCK, kul'tiviruemykh v trekhmernom gele v prisutstvii tsitokina HGF/SF.