Repair in Escherichia coli alkB mutants of abasic sites and 3-methyladenine residues in DNA.
Escherichia coli alkB mutants are sensitive to methyl methanesulfonate and dimethylsulphate, and are defective in the processing of methylated DNA. The function of the AlkB protein has not been determined. Here, we show that alkB mutants are not defective in repairing several different types of potentially toxic DNA lesions that are known to be generated by MMS, including apyrimidinic and apurinic sites, and secondary lesions that could arise at these sites (DNA-protein cross-links and DNA interstrand cross-links). Also, alkB mutants were not sensitive to MeOSO2-(CH2)2-Lex, a compound that alkylates the minor groove of DNA generating primarily 3-methyladenine.
Dinglay
S
S
Imperial Cancer Research Fund, Clare Hall Laboratories, Herts, UK.
Gold
B
B
Sedgwick
B
B
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D001080:Apurinic Acid / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D010582:Bacteriophage lambda / Q000187:drug effects / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D042926:Cytochrome P-450 CYP4A; D003577:Cytochrome P-450 Enzyme System / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000235:genetics*; D004269:DNA, Bacterial / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins; D007202:Indicators and Reagents / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D006899:Mixed Function Oxygenases / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation / Q000235:genetics; D009429:Netropsin / Q000031:analogs & derivatives / Q000494:pharmacology; D011119:Polynucleotides / Q000235:genetics / Q000378:metabolism; D013463:Sulfuric Acid Esters / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004279:DNA, Viral; D029968:Escherichia coli Proteins; D007202:Indicators and Reagents; D009153:Mutagens; D011119:Polynucleotides; D013463:Sulfuric Acid Esters; C001790:apyrimidinic acid; D001080:Apurinic Acid; D008769:Methylnitronitrosoguanidine; C101157:1-methyl-4-(((1-methyl-4-((3-((methylsulfonyl)oxy)-1-oxopropyl)amino)-1H-pyrrol-2-yl)carbonyl)amino)-N-propyl-1H-pyrrole-2-carboxamide; C025946:3-methyladenine; D009429:Netropsin; D003577:Cytochrome P-450 Enzyme System; D008741:Methyl Methanesulfonate; D006899:Mixed Function Oxygenases; C466427:AlkB protein, E coli; D042926:Cytochrome P-450 CYP4A; D000225:Adenine; C007482:dimethyl sulfate