title: Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis.
abstract: We describe the use of time-lapse fluorescence microscopy to visualize the movement of the DNA replication origin and terminus regions on the Bacillus subtilis chromosome during the course of the cell cycle. The origin and terminus regions were tagged with a cassette of tandem lac operator repeats and visualized through the use of a fusion of the green fluorescent protein to the LacI repressor. We have discovered that origin regions abruptly move apart towards the cell poles during a brief interval of the cell cycle. This movement was also seen in the absence of cell wall growth and in the absence of the product of the parB homologue spo0J. The origin regions moved apart an average distance of 1.4 microm in an 11 min period of abrupt movement, representing an average velocity of 0.17 microm min(-1), and reaching a maximum velocity of greater than 0.27 microm min(-1). The terminus region also exhibited a striking pattern of movement but not as far or a rapid as the origin region. These results provide evidence for a mitotic-like motor that is responsible for segregation of the origin regions of the chromosomes.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
authors.forename: C D
authors.initials: CD
authors.lastname: Webb
authors.forename: P L
authors.initials: PL
authors.lastname: Graumann
authors.forename: J A
authors.initials: JA
authors.lastname: Kahana
authors.forename: A A
authors.initials: AA
authors.lastname: Teleman
authors.forename: P A
authors.initials: PA
authors.lastname: Silver
authors.forename: R
authors.initials: R
authors.lastname: Losick
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D002453:Cell Cycle; D002473:Cell Wall; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004269:DNA, Bacterial / Q000648:ultrastructure*; D007091:Image Processing, Computer-Assisted; D008856:Microscopy, Fluorescence; D016296:Mutagenesis; D018741:Replication Origin; D012808:Sigma Factor; D014157:Transcription Factors
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus