Conformational stability and thermodynamics of folding of ribonucleases Sa, Sa2 and Sa3.
Ribonucleases Sa, Sa2, and Sa3 are three small, extracellular enzymes produced by different strains of Streptomyces aureofaciens with amino acid sequences that are 50% identical. We have studied the unfolding of these enzymes by heat and urea to determine the conformational stability and its dependence on temperature, pH, NaCl, and the disulfide bond. All three of the Sa ribonucleases unfold reversibly by a two-state mechanism with melting temperatures, Tm, at pH 7 of 48.4 degrees C (Sa), 41.1 degrees C (Sa2), and 47.2 degrees C (Sa3). The Tm values are increased in the presence of 0.5 M NaCl by 4.0 deg. C (Sa), 0.1 deg. C (Sa2), and 7.2 deg. C (Sa3). The Tm values are decreased by 20.0 deg. C (Sa), 31.5 deg. C (Sa2), and 27.0 deg. C (Sa3) when the single disulfide bond in the molecules is reduced. We compare these results with similar studies on two other members of the microbial ribonuclease family, RNase T1 and RNase Ba (barnase), and with a member of the mammalian ribonuclease family, RNase A. At pH 7 and 25 degrees C, the conformational stabilities of the ribonucleases are (kcal/mol): 2.9 (Sa2), 5.6 (Sa3), 6.1 (Sa), 6.6 (T1), 8.7 (Ba), and 9.2 (A). Our analysis of the stabilizing forces suggests that the hydrophobic effect contributes from 90 to 110 kcal/mol and that hydrogen bonding contributes from 70 to 105 kcal/mol to the stability of these ribonucleases. Thus, we think that the hydrophobic effect and hydrogen bonding make large but comparable contributions to the conformational stability of these proteins.
Pace
C N
CN
Department of Medical Biochemistry and Genetics, Texas A&M University, College Station 77843, USA.
Hebert
E J
EJ
Shaw
K L
KL
Schell
D
D
Both
V
V
Krajcikova
D
D
Sevcik
J
J
Wilson
K S
KS
Dauter
Z
Z
Hartley
R W
RW
Grimsley
G R
GR
D000595:Amino Acid Sequence; D004220:Disulfides / Q000737:chemistry; D007527:Isoenzymes / Q000737:chemistry*; D008969:Molecular Sequence Data; D011489:Protein Denaturation / Q000187:drug effects*; D017510:Protein Folding; D012260:Ribonucleases / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D013304:Streptomyces aureofaciens / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology
D004220:Disulfides; D007527:Isoenzymes; D012965:Sodium Chloride; D014508:Urea; D012260:Ribonucleases; C077002:ribonuclease Sa3