Cloning genes encoding receptors related to chemoattractant receptors.
We report the cloning of a novel human gene (GPR32) encoding a putative G-protein-coupled receptor (GPCR) of 356 amino acids and a related pseudogene psi GPR32. The deduced amino acid sequence of GPR32 shares 35-39% identity with members of the chemoattractant receptor family. psi GPR32 shares 93% nucleotide identity with GPR32. We identified a mouse EST encoding a putative GPCR (GPR33) of 309 amino acids. The deduced amino acid sequence of GPR33 shares 30-35% identity with members of the chemoattractant receptor family and 36% identity with the receptor encoded by GPR32. The human orthologue of GPR33 contains a single basepair substitution with respect to the mouse, resulting in the presence of an in-frame stop codon within the predicted second intracellular loop, demonstrating that it is a pseudogene. Through fluorescence in situ hybridization and physical mapping of YACs, both GPR32 and psi GPR32 were mapped to chromosomal 19, region q13.3, while psi GPR33 was mapped to chromosome 14q12.
Marchese
A
A
Department of Pharmacology, University of Toronto, Ontario, Canada.
Nguyen
T
T
Malik
P
P
Xu
S
S
Cheng
R
R
Xie
Z
Z
Heng
H H
HH
George
S R
SR
Kolakowski
L F
LF
O'Dowd
B F
BF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002883:Chromosomes, Human, Pair 14; D002888:Chromosomes, Human, Pair 19; D003001:Cloning, Molecular; D018388:Codon, Terminator; D019204:GTP-Binding Proteins; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008969:Molecular Sequence Data; D011544:Pseudogenes; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000235:genetics*; D043562:Receptors, G-Protein-Coupled
D018388:Codon, Terminator; C114479:GPR32 protein, human; C114478:Gpr33 protein, mouse; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins