Increase in expression and activity of thrombomodulin in term human syncytiotrophoblast microvilli.
A comparative study of thrombomodulin (TM), a potent natural anticoagulant, was performed in first trimester and term human placentae. Immunoreactive TM was observed on fetal vascular endothelium and syncytiotrophoblast at both gestational ages. Staining was stronger in term than in early placentae, particularly along the microvillous apical membrane of the syncytiotrophoblast. Similarly, a higher level of TM mRNA was detected by RT-PCR (P<0.02) and Northern blot analysis in extracts of whole term placentae. The localization of TM on syncytial microvilli was confirmed by electron microscopy after immunogold labelling. When isolated microvilli were compared at both gestational ages; a significant 2.3-fold increase in TM protein was observed in term microvilli as compared to first trimester microvilli by Western blot analysis (P<0.005) and ELISA (P<0.05). This higher level of TM in term microvilli was associated with an increase in its ability to activate protein C, from 3.7 +/- 1.2 to 8.7 +/- 4.2 mOD/min/microg protein +/- s.d. (P<0.01) in first trimester and term microvilli, respectively. The modulation of biologically active TM at the syncytial membrane exposed to maternal blood according to the length of gestation suggests that TM may be involved both in maternal haemostasis within the intervillous spaces, and also in the trophoblast differentiation process.
Fazel
A
A
INSERM U427, Université René Descartes, Faculté des Sciences Pharmaceutiques et Biologiques, Paris, France.
Vincenot
A
A
Malassiné
A
A
Soncin
F
F
Gaussem
P
P
Alsat
E
E
Evain-Brion
D
D
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron; D008871:Microvilli / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011263:Pregnancy Trimester, Third; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018180:Thrombomodulin / Q000235:genetics* / Q000378:metabolism*; D014327:Trophoblasts / Q000378:metabolism* / Q000648:ultrastructure
D017931:DNA Primers; D012333:RNA, Messenger; D018180:Thrombomodulin