Separation of ergot alkaloids and their epimers and determination in sclerotia by capillary electrophoresis.
Capillary electrophoresis has been shown to be a very useful analysis technique for secondary metabolites of plants. In the present study a method is described for the qualitative and quantitative determination of ergot alkaloids and their epimers. The extraction from the biological matrix yields recoveries of 50-97%, depending on the individual alkaloid. Using a mixture of 20 mM beta-cyclodextrin (CD), 8 mM gamma-CD, 2 M urea and 0.3% (w/w) poly(vinyl alcohol) to phosphate buffer at pH 2.5 the simultaneous separation of all analytes was achieved. A 37 cm (30 cm) fused-silica capillary, at a voltage of 25 kV and a temperature of 20 degrees C, was used for the analysis. Overall analysis time for the separation was 12 min. The limit of detection of the alkaloids using UV detection at 214 nm can be improved 30-fold to about 9.10(-8) M when laser-induced fluorescence detection is applied.
Frach
K
K
Institute of Pharmaceutical Chemistry, University of Münster, Germany.
Blaschke
G
G
D002021:Buffers; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D004876:Ergot Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D010710:Phosphates; D012995:Solubility; D014508:Urea; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins
D002021:Buffers; D003505:Cyclodextrins; D004876:Ergot Alkaloids; D010710:Phosphates; D047392:beta-Cyclodextrins; D047408:gamma-Cyclodextrins; D014508:Urea; C031215:betadex; C023792:gamma-cyclodextrin