Nodular localized cutaneous amyloidosis: further demonstration of monoclonality of infiltrating plasma cells in four additional Japanese patients.
Nodular localized cutaneous amyloidosis (NLCA) is a disorder characterized by deposition of amyloid derived from immunoglobulin light chains. We used semi-nested polymerase chain reaction (PCR) to analyse archival paraffin-embedded sections from a previous patient and from four additional, previously reported patients with NLCA to determine whether involvement of monoclonal plasma cells is a universal feature of this condition. The semi-nested PCR analysis revealed one or two amplified bands, around 100-120 bp, for all five cases of NLCA, although the yields varied from case to case. These results suggest that clonal expansion of plasma cells in NLCA may occur locally.
Hagari
Y
Y
Department of Dermatology, Faculty of Medicine, Tottori University, Yonago, Japan. yohagari@grape.med.tottori-u.ac.jp
Mihara
M
M
Konohana
I
I
Ueki
H
H
Yamamoto
O
O
Koizumi
H
H
D000328:Adult; D000368:Aged; D000686:Amyloidosis / Q000473:pathology*; D002999:Clone Cells / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016612:Paraffin Embedding; D010950:Plasma Cells / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012871:Skin Diseases / Q000473:pathology*