title: Cleavage of atrial natriuretic peptide by a kidney membrane-bound carboxypeptidase A.
abstract: An enzymatic activity that cleaved the C-terminal Tyr of ANP (1-28) was characterized in human kidney microvillar membranes by using 125I-labeled rat ANP as substrate. This activity was inhibited by potato carboxypeptidase inhibitor (PCI) and 1.10 phenanthroline, suggesting that it corresponded to a metallo-carboxypeptidase. Solubilization experiments indicated that the carboxypeptidase activity could be recovered in the supernatant after 1% Triton X-100 extraction. As separation by ion exchange chromatography revealed several peaks of enzyme activity, PCI coupled to Sepharose was used for purification. This step resulted in a single protein band at 30 kDa, as analyzed by SDS-PAGE.
authors.affiliation: Laboratoire de Chimie Biologique, Université de Mons-Hainaut, Mons, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Michel
authors.forename: J
authors.initials: J
authors.lastname: Nortier
authors.forename: A
authors.initials: A
authors.lastname: Humblet
authors.forename: C
authors.initials: C
authors.lastname: Paradis
authors.forename: E
authors.initials: E
authors.lastname: De Prez
authors.forename: M
authors.initials: M
authors.lastname: Deschodt-Lanckman
mesh_terms: D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism*; D043422:Carboxypeptidases A; D002847:Chromatography, Agarose; D006801:Humans; D007457:Iodine Radioisotopes; D007672:Kidney Cortex / Q000378:metabolism* / Q000648:ultrastructure; D008871:Microvilli / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D012995:Solubility
chemical_list: D007457:Iodine Radioisotopes; D011480:Protease Inhibitors; D009320:Atrial Natriuretic Factor; D002268:Carboxypeptidases; D043422:Carboxypeptidases A