[Effects of glucose and TGF-beta 1 on the proliferation of cultured human peritoneal mesothelial cells].
The exfoliation and decrease is peritoneal mesothelial cells and the presence of interstitium hyperplasia are often observed in peritoneal membrane dysfunction caused by long-term peritoneal dialysis. The suppression of peritoneal mesothelial cell proliferation may be the cause of these phenomena. The objective of this study is to clarify the mechanism by which highly concentrated glucose of peritoneal dialysis fluid inhibits mesothelial cell proliferation. We examined the effect of highly concentrated glucose in the medium on human peritoneal mesothelial cell proliferation and TGF-beta 1 mRNA expression. The effect of Ham's F12 media containing various levels of glucose concentration was compared with that of normal medium. We investigated human peritoneal mesothelial cell proliferation by [3H] thymidine incorporation assay and TGF-beta 1 mRNA expression on human mesothelial cells by the RT-PCR method. The suppression effect of glucose and TGF-beta 1 on human peritoneal mesothelial cell proliferation was dose-dependent (glucose; 0-5%, TGF-beta 1; 0-1000 pg/ml). TGF-beta 1 mRNA of cells in 4% glucose medium was greater than that in the control medium. The glucose-induced suppression of human peritoneal mesothelial cell proliferation was relieved by LAP and TGF-beta neutralizing antibody. In conclusion, TGF-beta 1 may play a critical role in inhibiting mesothelial cell proliferation in media with highly concentrated glucose.
Yonekawa
S
S
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D010531:Peritoneal Dialysis, Continuous Ambulatory / Q000009:adverse effects; D010537:Peritoneum / Q000166:cytology*; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000502:physiology
D016212:Transforming Growth Factor beta; D005947:Glucose