Aldosterone overproduction and CYP11B2 mRNA overexpression in vessels of spontaneously hypertensive rats.
Aldosterone is synthesized in extra-adrenal tissues such as blood vessels and brain. Damage to blood vessels could play a crucial role in perpetuating hypertension. In this study, we determined both aldosterone production and aldosterone synthase gene-CYP11B2 mRNA expression in vessels of spontaneously hypertensive rats (SHR). The results showed that aldosterone was overproduced in the vasculature of SHR, by means of ex vivo mesenteric artery perfusion, HPLC, and RIA, and that CYP11B2 mRNA expression was upregulated in aortas of SHR, confirmed by RT-PCR and Southern blot.
Wu
P
P
Department of Cardiology, Nanfang Hospital, The First Military Medical University, Guangzhou, People's Republic of China.
Guo
Z
Z
Zhang
Y
Y
Liu
Y
Y
Liang
X
X
Zhang
R
R
Lai
W
W
Takeda
Y
Y
Isamu
M
M
Takeda
R
R
D000450:Aldosterone / Q000096:biosynthesis*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D001158:Arteries / Q000378:metabolism*; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D015870:Gene Expression; D006973:Hypertension / Q000378:metabolism*; D008638:Mesenteric Arteries / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D017208:Rats, Wistar
D012333:RNA, Messenger; D000450:Aldosterone; D019405:Cytochrome P-450 CYP11B2