Glutaredoxin is a direct target of oncogenic jun.
We have analysed differential gene expression in v-jun-transformed chicken embryo fibroblasts (CEF) compared to normal CEF by using the directional tag PCR subtraction method. From a first generation of putative Jun targets four clones were selected for study; they are upregulated in jun-transformed cells. Three of these clones showed homology to known genes: glutaredoxin, growth associated protein (GAP)-43/neuromodulin, and phenobarbital-induced cytochrome P450. The expression of these genes was analysed in fibroblasts transformed by various oncogenes. Expression of the glutaredoxin mRNA could be induced by a Jun-estrogen receptor chimaera in the absence of de novo protein biosynthesis. Based on this observation we conclude that glutaredoxin is a direct target of v-Jun.
The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, California 92037, USA.
M E
ME
Goller
J S
JS
Iacovoni
P K
PK
Vogt
U
U
Kruse
D000818:Animals; D002642:Chick Embryo; D019922:GAP-43 Protein / Q000235:genetics; D005786:Gene Expression Regulation; D054477:Glutaredoxins; D016759:Oncogene Protein p65(gag-jun) / Q000235:genetics*; D010088:Oxidoreductases; D011506:Proteins / Q000235:genetics*
D019922:GAP-43 Protein; D054477:Glutaredoxins; D016759:Oncogene Protein p65(gag-jun); D011506:Proteins; D010088:Oxidoreductases