On the size of pores arising in erythrocytes under the action of detergents.
The size of pores arising in human erythrocytes under the action of two detergents (Triton X-100 and sodium dodecyl sulfate) and causing the slow component of hemolysis was estimated by the method of osmotic protectors. The pore diameters were found to be about 40 A. The pores responsible for the fast component of hemolysis induced by sodium dodecyl sulfate were shown to be of greater size and even molecules of polyethylene glycol 4000 could pass through them. The unusual decrease. In the rate and percentage of this hemolytic component was caused by the side action of the protectors, i.e., by the acceleration of erythrocyte vesiculation, a process that competed with pore formation. Vesiculation protected erythrocytes against fast and slow detergent-induced hemolysis. It is shown that the method of osmotic protectors can not be used for estimation of pore size in fast hemolysis by sodium dodecyl sulfate. The application of this method for hemolysis by other amphiphilic compounds is discussed.
Institute of Photobiology, Academy of Sciences of the Republic of Belarus, Minsk, Republic of Belarus. volot%bas31.basnet.minsk.by@demos.su
O A
OA
Senkovich
E A
EA
Chernitsky
D003902:Detergents / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000502:physiology; D004912:Erythrocytes / Q000187:drug effects / Q000502:physiology*; D006461:Hemolysis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D017830:Octoxynol / Q000494:pharmacology*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D019287:Zinc Sulfate / Q000494:pharmacology
D003902:Detergents; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D019287:Zinc Sulfate; D017830:Octoxynol