Synthesis of terminal disaccharide elements corresponding to the Ogawa and Inaba antigenic determinant from Vibrio cholerae O1.
Vibrio cholerae O1 LPS terminal mono- and disaccharide elements were synthesized by reduction of the azido group in several 4-amino-4,6-dideoxy-D-mannose mono- and disaccharide derivatives, followed by coupling with 2, 4-di-O-acetyl-3-deoxy-L-glycero-tetronic acid in the presence of 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline. This compound represents a useful model in order to elucidate the size of the epitopes which define Ogawa and Inaba serotypes from Vibrio cholerae O1.
Laboratory of Synthetic Antigens, Facultad de Química, Universidad de La Habana, Cuba.
A
A
Arencibia-Mohar
A
A
Ariosa-Alvarez
O
O
Madrazo-Alonso
E
E
González Abreu
L
L
García-Imia
G
G
Sierra-González
V
V
Verez-Bencomo
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D002236:Carbohydrate Conformation; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D006885:Hydroxybutyrates / Q000138:chemical synthesis* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D051379:Mice; D009005:Monosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D009904:Optical Rotation; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology*
C114469:2,4-di-O-acetyl-3-deoxy-glycero-tetronic acid; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004187:Disaccharides; D000939:Epitopes; D006885:Hydroxybutyrates; D008070:Lipopolysaccharides; D009005:Monosaccharides