A role for interleukin 4 in production of matrix metalloproteinase 1 by human aortic smooth muscle cells.
Effect of interleukin 4 (IL-4) on the production of matrix metalloproteinase 1 (MMP-1) by normal and immortalized human intimal smooth muscle cells (SMC) was investigated. The production of the precursors of MMP-1 by intimal SMC was enhanced in a dose-dependent manner by addition of IL-4 to the culture medium, whereas the cytokine also showed an inhibitory effect on DNA synthesis in the cells. In addition, mRNA of IL-4 was found in the atherosclerotic and nonatherosclerotic areas of the intima. Although the production of MMP-1 and the proliferation of SMC are thought to play an important role in reconstruction of the intima during atherogenesis, our results suggest a possible role of IL-4 induced MMP-1 in inhibiting tissue remodeling caused by a variety of arterial disorders including atherosclerosis.
Department of Pathology, University of Occupational and Environmental Health, School of Medicine, Kitakyusyu, Japan.
T
T
Sasaguri
N
N
Arima
A
A
Tanimoto
S
S
Shimajiri
T
T
Hamada
Y
Y
Sasaguri
D001011:Aorta / Q000201:enzymology / Q000378:metabolism; D001161:Arteriosclerosis / Q000378:metabolism; D002478:Cells, Cultured; D017364:Collagenases / Q000096:biosynthesis* / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006801:Humans; D015847:Interleukin-4 / Q000378:metabolism / Q000494:pharmacology*; D020781:Matrix Metalloproteinase 1; D009131:Muscle, Smooth, Vascular / Q000201:enzymology / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis
D012333:RNA, Messenger; D015847:Interleukin-4; D017364:Collagenases; D020781:Matrix Metalloproteinase 1