Localization of Dlg at the mammalian neuromuscular junction.
Recent studies have begun to elucidate the localization of ion channels and receptors in central nervous system synapses. A family of proteins containing PDZ domains has been suggested to play essential roles in these processes. PSD-95 and chapsyn-110 have been implicated in the clustering of Shaker K+ channels and NMDA receptors in the mammalian brain, and Dlg plays a role in the clustering of Shaker K+ channels at the Drosophila neuromuscular junction (NMJ). We have explored whether Dlg might participate in mammalian NMJ organization. We demonstrate that Dlg is expressed in muscle and co-localizes with utrophin at the post-synaptic face of the mammalian NMJ. Dlg may therefore be important for establishing or maintaining the organization of protein complexes at the mammalian NMJ.
Rafael
J A
JA
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Hutchinson
T L
TL
Lumeng
C N
CN
Marfatia
S M
SM
Chishti
A H
AH
Chamberlain
J S
JS
D000818:Animals; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification; D019085:Fluorescent Antibody Technique, Direct; D005804:Genes, Lethal / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D049411:Utrophin
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C483600:Utrn protein, mouse; D049411:Utrophin; D012313:RNA; D004247:DNA