Combinatorial protein design by in vitro recombination.
DNA recombination is a powerful engine for the creation of new phenotypes. Recently, methods for in vitro DNA recombination (DNA shuffling) have been developed and applied to the evolution of novel molecules in the laboratory. An exciting new development is the shuffling of homologous genes to create diversity for directed evolution.
Giver
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Division of Chemistry and Chemical Engineering 210-41, California Institute of Technology, Pasadena 91125, USA.
Arnold
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FH
D004247:DNA / Q000235:genetics; D004274:DNA, Recombinant; D019020:Directed Molecular Evolution; D015195:Drug Design; D014644:Genetic Variation; D014176:Protein Biosynthesis; D011506:Proteins / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D011995:Recombination, Genetic
D004274:DNA, Recombinant; D011506:Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D004247:DNA