In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform modification of all cells in an apically growing, non-uniform organism, and subsequent visualization of protected areas by ligation-mediated PCR.
IBTM, Technical University Vienna, Getreidemarkt 9/172-5, A-1060 Vienna, Austria, Institut für Biochemie,Vienna Biocenter, Dr. Bohr Gasse 9, A-1030 Vienna, Austria.
M F
MF
Wolschek
F
F
Narendja
J
J
Karlseder
C P
CP
Kubicek
C
C
Scazzocchio
J
J
Strauss
D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001234:Aspergillus niger / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D018983:DNA Footprinting / Q000379:methods*; D019175:DNA Methylation; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D003904:Mitosporic Fungi / Q000235:genetics* / Q000378:metabolism*; D015345:Oligonucleotide Probes / Q000235:genetics; D010408:Penicillium chrysogenum / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding
D004271:DNA, Fungal; D005656:Fungal Proteins; D015345:Oligonucleotide Probes