Determination of total cysteamine in human serum by a high-performance liquid chromatography with fluorescence detection.
A convenient, reliable and rapid method for determination of total cysteamine in human plasma by high-performance liquid chromatography with fluorescence detection is reported. This assay involves reduction of samples with dithiothreitol, derivatization of total cysteamine by addition of monobromobimane and protein precipitation by perchloric acid. The calibration curve was linear in the range 2-150 nmol ml-1 and the detection limit was 0.5 nmol ml-1. This method was successfully applied for a pharmacokinetic study of three cysteamine derivatives in healthy volunteers without any interference from coexisting substances.
Laboratoire Central d'Analyses, Pharmacie Centrale des Hôpitaux, Paris, France.
M
M
Stachowicz
B
B
Lehmann
A
A
Tibi
P
P
Prognon
V
V
Daurat
D
D
Pradeau
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003543:Cysteamine / Q000097:blood* / Q000493:pharmacokinetics; D004220:Disulfides / Q000737:chemistry; D006801:Humans; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D004220:Disulfides; D003543:Cysteamine