Protection of DNA by salts against thermodegradation at temperatures typical for hyperthermophiles.
The effect of physiological concentrations of KCl and MgCl2 on the chemical stability of double-stranded and single-stranded DNA has been studied at temperatures typical for hyperthermophiles. These tow salts protect both double and single-stranded DNA against heat-induced cleavage by inhibiting depurination. High KCl concentration also protect DNA cleavage at apurinic sites, while high MgCl2 concentrations stimulate this cleavage. It has been previously proposed that salt protects double-stranded DNA against depurination by stabilizing the double helix. However, the inhibition of the depurination of single-stranded DNA by KCl and MgCl2 indicates that this effect is more probably due to a direct interaction of salts with purine nucleotides. These results suggest that the number and nature of heat-induced DNA lesions which have to be repaired might be quite different from one hyperthermophile to another, depending on their intracellular salt concentration. High salt concentrations might be also useful to protect DNA in long polymerase chain reaction (PCR) experiments and for long-term preservation.
Marguet
E
E
Institut de Génétique et Microbiologie, Université Paris-Sud, CNRS, URA 1354, Bât. 409, Orsay, France.
Forterre
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P
D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000737:chemistry*; D015636:Magnesium Chloride; D011189:Potassium Chloride; D013696:Temperature
D004277:DNA, Single-Stranded; D015636:Magnesium Chloride; D011189:Potassium Chloride; D004247:DNA