Structural changes in human tear lipocalins associated with lipid binding.
Structural and conformational changes in tear lipocalins were detected in association with ligand binding and release. Circular dichroism measurements demonstrated that ligand binding induces beta structure formation, aromatic side chain asymmetry, and a more rigid state in tear lipocalins (TL). The exposure of the tyrosyl component is less in apo-TL than in holo-TL. The sole tryptophan residue, Trp17, is buried in both holo- and apo-TL. The steady state exposure of Trp17 is the same in holo- and apo-TL, but the dynamic exposure is two-fold greater in apo-TL. Maneuvers to unfold the protein with urea or incubation in an acidic environment resulted in increased exposure of aromatic amino acids. Electron paramagnetic resonance studies verified that lipids are liberated from TL in an acidic environment. Acidic pH promotes conformational changes in TL involving aromatic residues, particularly the conserved residue Trp17. These changes are associated with lipid release. The liberation of lipid from the cavity of TL under acidic conditions involves a molten globule state of the protein. We postulate that TL, exposed to the steep surface pH gradient that exists at lipid-aqueous interfaces, would release lipid in association with a molten globule transition. The data suggest a plausible regulatory mechanism for lipid delivery from lipocalins at the tear film surface.
Gasymov
O K
OK
Departments of Pathology and Ophthalmology, UCLA School of Medicine, 100 Stein Plaza, Los Angeles, CA 90095, USA.
Abduragimov
A R
AR
Yusifov
T N
TN
Glasgow
B J
BJ
D000817:Anilino Naphthalenesulfonates; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D005136:Eye Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D050356:Lipid Metabolism; D054835:Lipocalin 1; D011489:Protein Denaturation; D017433:Protein Structure, Secondary; D013050:Spectrometry, Fluorescence; D013666:Tears / Q000737:chemistry*
D000817:Anilino Naphthalenesulfonates; D002352:Carrier Proteins; D005136:Eye Proteins; C518443:LCN1 protein, human; D008024:Ligands; D054835:Lipocalin 1; C042434:tear proteins; C027132:1-anilino-8-naphthalenesulfonate