Detection of varicella-zoster virus genome in the vitreous humor from two patients with acute retinal necrosis; lacking or having a PstI cleavage site.
Using polymerase chain reaction, we detected the varicella-zoster virus genome in the vitreous humor of two patients with clinically diagnosed acute retinal necrosis. One of the two cases was thought to be caused by infection with a varicella-zoster virus lacking a PstI cleavage site. We could not find any clinical differences between the two substrains. The presence of a PstI cleavage site on the varicella-zoster virus genome might not be associated with the occurrence of acute retinal necrosis.
Yanagida Eye Clinic, Shizuoka, Japan.
K
K
Mochizuki
H
H
Matsushita
Y
Y
Hiramatsu
K
K
Yanagida
D000368:Aged; D001082:Aqueous Humor / Q000821:virology; D015342:DNA Probes / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005500:Follow-Up Studies; D016679:Genome, Viral; D006563:Herpes Zoster Ophthalmicus / Q000601:surgery / Q000821:virology*; D014645:Herpesvirus 3, Human / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015882:Retinal Necrosis Syndrome, Acute / Q000601:surgery / Q000821:virology*; D014821:Vitrectomy; D014822:Vitreous Body / Q000601:surgery / Q000821:virology*
D015342:DNA Probes; D004279:DNA, Viral; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific