Beta-carotene hydroxylase gene from the cyanobacterium Synechocystis sp. PCC6803.
The ORF sll1468 of Synechocystis sp. PCC6803 was identified as a gene for beta-carotene hydroxylase by functional complementation in a beta-carotene-producing Escherichia coli. The gene product of ORF sll1468 added hydroxyl groups to the beta-ionone rings of beta-carotene (beta, beta-carotene) to form zeaxanthin (beta, beta-carotene-3,3'-diol). This newly identified beta-carotene hydroxylase does not show overall amino acid sequence similarity to the known beta-carotene hydroxylases. However, it showed significant sequence similarity to beta-carotene ketolases of marine bacteria and a green alga.
Masamoto
K
K
Biological Laboratory, Faculty of Education, Kumamoto University, Japan.
Misawa
N
N
Kaneko
T
T
Kikuno
R
R
Toh
H
H
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D002338:Carotenoids / Q000096:biosynthesis; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010105:Oxygenases / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D002338:Carotenoids; D006899:Mixed Function Oxygenases; D010105:Oxygenases; C093259:beta-carotene ketolase; C079232:beta-carotene hydroxylase