Evaluation of the MAP-1B ELISA for cowdriosis with field sera from livestock in Zimbabwe.
The Map 1B (Senegal) antigen-based indirect ELISA was evaluated in Zimbabwe with field sera from heartwater-free and heartwater-endemic areas. Of 205 sheep sera samples from a heartwater-free area, 34 were negative and 171 were positive by immunoblotting. These 171 samples were classified as false positives. Of the same 205 samples, 199 were negative and only 6 were positive by the MAP 1B ELISA. Of 72 cattle samples tested from a similar area, 71 were negative, with only 1 sample positive by MAP 1B ELISA. By immunoblotting, 43 of 72 cattle sera were negative and 29 were positive. Of the 46 goat samples tested from a heartwater-free area, only 2 were positive by the MAP 1B ELISA. Based on these results, the MAP 1B ELISA was more specific for heartwater than the immunoblotting assay. Of 96 and 282 cattle sera analyzed from heartwater-endemic farms in the lowveld and highveld of Zimbabwe, respectively, approximately 33% were positive by the MAP 1B ELISA. Goat sera from the same farms had a higher sero-prevalence (> 90%). The implications of these results for serodiagnosis of heartwater using the MAP 1B ELISA will be discussed.
Mahan
S M
SM
UF/USAID/SADC Heartwater Research Project, Harare, Zimbabwe.
Semu
S M
SM
Peter
T F
TF
Jongejan
F
F
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000276:immunology; D016995:Ehrlichia ruminantium; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005189:False Positive Reactions; D005843:Geography; D006357:Heartwater Disease / Q000175:diagnosis* / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D015030:Zimbabwe
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C113540:MAP1-B protein, Cowdria ruminantium; D008565:Membrane Proteins