Acute effects of 3,4-dichloroaniline on blood of male Wistar rats.
In this work we investigated the acute effects of 3,4-dichloroaniline (DCA) on the changes of specific biochemical and cellular blood parameters using male Wistar rats, 24 hours after the intraperitoneal administration of five different doses of DCA (0, 81, 162, 324, 486, and 568 mg/Kg of body weight). We also evaluated the dose-dependent toxicity of DCA on the ratio "organ weight:total body weight" for kidney, liver, spleen and thymus, and on the changes of morphological characteristics of the spleen. The LOEL (lowest observed effect level) value for spleen and thymus injury was 324 mg DCA/Kg. The cellular blood parameters affected by DCA include: methemoglobin concentration, the number of circulating leukocytes and the number and size of platelets. The relative sensitivity of all the analysis used to assess the acute toxicity of DCA was evaluated by comparing their ED50 values, which ranged from 224 to 837 mg DCA/Kg. We concluded that the most sensitive endpoint to measure the acute toxicity of DCA was the methemoglobin formation. Platelet counts, urea and creatinine showed ED50 values slightly higher than methemoglobin (329-367 mg/kg).
Departamento de Estudos de Populações, Universidade do Porto, Portugal.
L
L
Guilhermino
A M
AM
Soares
A P
AP
Carvalho
M C
MC
Lopes
D000814:Aniline Compounds / Q000633:toxicity*; D000818:Animals; D001774:Blood Chemical Analysis; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology; D018675:Toxicity Tests / Q000592:standards
D000814:Aniline Compounds; C014464:3,4-dichloroaniline