Sensitive high-performance liquid chromatographic determination of arbidol, a new antiviral compound, in human plasma.
A highly sensitive and selective HPLC method was developed and validated for the determination of arbidol in human plasma. The method involves the liquid-liquid extraction of drug and internal standard from plasma with tert.-butyl methyl ether followed by evaporation and reconstitution in mobile phase. UV detection was done at 315 nm. The limit of quantification for arbidol in plasma was 0.005 microgram/ml. Linearity in plasma was proven over the whole calibration range (10.2-0.005 micrograms/ml). The method was validated according to GLP guidelines and its suitability was demonstrated by analysis of samples from a pharmacokinetic study.
Heumann Pharma, Nürnberg, Germany.
R
R
Metz
P
P
Muth
M
M
Ferger
V G
VG
Kukes
H
H
Vergin
D000998:Antiviral Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D005615:Freezing; D006801:Humans; D007202:Indicators and Reagents; D007211:Indoles / Q000097:blood*; D015203:Reproducibility of Results; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides
D000998:Antiviral Agents; D007202:Indicators and Reagents; D007211:Indoles; D012996:Solutions; C086979:umifenovir; D013440:Sulfides