Juvenile arthritis, HLA-A2 and binding of DEK oncogene-peptides.
Previous studies have shown that susceptibility to Pauciarticular Juvenile Arthritis is associated with HLA-A*0201. Recently, autoantibodies against the protein of the DEK oncogene have been found in sera of patients with this disease. If T cells are involved in the pathogenesis of joint lesions, it is possible that they target autoantigens presented by HLA-A*0201. Therefore, we investigated whether DEK-derived peptides can bind efficiently to HLA-A*0201. Nonameric peptides selected considering anchor positions 2 and 9, and preferred amino acids at other positions, were incubated either with the human TAP-deficient cell line 174CEM.T2 (T2) or with the homozygous B cell line JESTHOM (A*0201, B*2705, Cw1), previously depleted of endogenous peptides. Binding was measured as the increase of detection of fully assembled, HLA-A*0201 molecules by flow cytometry with the anti-HLA-A2 monoclonal antibody (mAb) BB7.2. Three out of ten selected DEK-derived peptides showed binding to HLA-A*0201, which was peptide concentration-dependent (1 microM to 100 microM). DEK155-163 (AMLKSICEV), which also has two preferred amino acid residues at positions 6 and 8, yielded the highest binding. DEK163-171 (VLDLERSGV) and DEK72-80 (SLQREPFTI), which also has one preferred amino acid residue at position 8, also were able to bind to HLA-A*0201. Furthermore, peptide-induced, fully assembled, HLA-A*0201 molecules were immunoprecipitated with the BB7.2 mAb from metabolically-labeled T2 cells incubated with DEK72-80, DEK155-163, and DEK163-171. A faint band was observed in the immunoprecipitates of cells incubated with DEK65-73 (it carries a preferred amino acid residue at position 6), suggesting that this peptide interacts weakly with HLA-A*0201. These results indicate that several nonameric peptides derived from the DEK protein can bind to HLA-A 0201 and suggest that the complexes formed may be able to stimulate CD8+ T cells in patients with Pauciarticular Juvenile Arthritis.
Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8886, USA.
L
L
Forero
N W
NW
Zwirner
C W
CW
Fink
M A
MA
Fernández-Viña
P
P
Stastny
D001171:Arthritis, Juvenile / Q000276:immunology* / Q000378:metabolism*; D001324:Autoantigens / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites / Q000276:immunology; D002868:Chromosomal Proteins, Non-Histone; D015789:HLA-A2 Antigen / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D000075223:Poly-ADP-Ribose Binding Proteins; D011485:Protein Binding / Q000276:immunology
D001324:Autoantigens; D002868:Chromosomal Proteins, Non-Histone; C084738:DEK protein, human; D015789:HLA-A2 Antigen; D009842:Oligopeptides; D015513:Oncogene Proteins; D000075223:Poly-ADP-Ribose Binding Proteins