The effect of prostaglandin E1 on in vitro transcription of sperm chromatin, isolated from patients with azoospermia, teratospermia and chronic prostatitis.
We have investigated the influence of Prostaglandin E1 on the in vitro transcription of chromatin, isolated from spermatozoa of patients suffering from different pathologies, leading to infertility, namely, azoospermia, teratospermia and chronic prostatitis. Our studies indicate that prostaglandin E1 has a stimulatory effect both on in vitro transcription, on the number of RNA polymerase molecules and the polyribonucleotide elongation rates as compared to sperm chromatin from healthy patients. The results on the incorporation of alpha-32P-ATP in to RNA in the presence and absence of Prostaglandin E1 correlate well with the data on the number of actively transcribing RNA polymerase molecules and the rate of RNA elongation, which might be due to low levels of prostaglandin E1 in human semen.
Institute of Molecular Biology, Bulg. Acad. Sci., Sofia, Bulgaria.
G
G
Pironcheva
K
K
Miteva
G C
GC
Russev
C
C
Vaisberg
S
S
Zlatarev
D000527:Alprostadil / Q000494:pharmacology*; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism*; D002908:Chronic Disease; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008297:Male; D009845:Oligospermia / Q000378:metabolism*; D011131:Polyribonucleotides / Q000378:metabolism; D011472:Prostatitis / Q000378:metabolism*; D012016:Reference Values; D013094:Spermatozoa / Q000002:abnormalities / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects*
D002843:Chromatin; D011131:Polyribonucleotides; D012321:DNA-Directed RNA Polymerases; D000527:Alprostadil