Co-expression of p75NTR- and calbindin-immunoreactivity in cholinergic neurons of the raccoon basal forebrain.
The cholinergic system of the basal forebrain is involved in the modulation of sensory information. This has previously been investigated in the raccoon, an animal especially interesting because of its highly developed somatosensory cortex. The present study focused on the co-expression of the low-affinity neurotrophin receptor p75NTR and calbindin in cholinergic neurons of the raccoon basal forebrain and neostriatum. Carbocyanine immunofluorescence double labelling revealed the co-localization of choline acetyltransferase and p75NTR as well as calbindin in a large portion of basal forebrain neurons, but not in the neostriatum. In contrast, immunolabelling of two other calcium-binding proteins, parvalbumin and calretinin, was found exclusively in non-cholinergic neurons.
Department of Physiology and Biophysics, Dalhousie University, Halifax, NS, Canada.
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L
Tremere
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Brückner
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K
Brauer
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DD
Rasmusson
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R
Poethke
W
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Härtig
D000818:Animals; D064026:Calbindins; D002795:Choline O-Acetyltransferase / Q000032:analysis; D002799:Cholinergic Fibers / Q000737:chemistry* / Q000201:enzymology; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D017072:Neostriatum / Q000737:chemistry; D010320:Parvalbumins / Q000032:analysis; D016548:Prosencephalon / Q000737:chemistry*; D011821:Raccoons / Q000502:physiology*; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis*; D064030:S100 Calcium Binding Protein G / Q000032:analysis*
D064026:Calbindins; D010320:Parvalbumins; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D064030:S100 Calcium Binding Protein G; D002795:Choline O-Acetyltransferase