Immunochemical characterization of Bacteroides vulgatus cell-surface antigens.
Capsular polysaccharide (CPS) and lipopolysaccharide (LPS) antigens extracted from three Bacteroides vulgatus strains (serotype specific strain, and two others isolated from normal gut flora and gut of a diabetic patient) were studied. Chemical analysis of CPS and purified LPS antigens was performed. The compositions of sugars, amino acids and fatty acids were shown to be different for each antigen. Rabbit antisera against whole bacterial cells were prepared. All antigens proved to be active in immunoelectrophoresis, SDS-PAGE and immunoblot tests. Several cross reactions in these assays were observed.
Department of Clinical Bacteriology, Medical Academy, Warsaw, Poland.
G S
GS
Rouyan
W
W
Kaca
F
F
Meisel-Mikołajczyk
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000737:chemistry / Q000276:immunology; D016667:Bacterial Capsules / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001439:Bacteroides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015151:Immunoblotting; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits
D000907:Antibodies, Bacterial; D000954:Antigens, Surface; D008070:Lipopolysaccharides