Competitive PCR for quantification of BM5d proviral DNA in mice with AIDS.
Murine AIDS in C57BL/6 mice is caused by a unique mixture of murine leukemia viruses. We report the use of a competitive PCR to detect and quantitate BM5d proviral DNA. This assay allowed discrimination among endogenous wild-type murine retroviruses and BM5d sequences. Furthermore, the method was subsequently used to evaluate the amount of BM5d in infected mice and in infected AZT (zidovudine)-treated mice, providing an effective way to quantitatively evaluate drug efficacy in the murine AIDS model.
Giorgio Fornainl Institute of Biochemistry, University of Urbino, Italy.
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A
Casabianca
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Vallanti
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M
Magnani
D000818:Animals; D004279:DNA, Viral / Q000032:analysis*; D018628:Gene Dosage; D009052:Leukemia Virus, Murine / Q000235:genetics*; D008198:Lymph Nodes / Q000821:virology; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000188:drug therapy / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics*; D015215:Zidovudine / Q000627:therapeutic use
D004279:DNA, Viral; D015215:Zidovudine