OMEC II: a new ovine mammary epithelial cell line.
We have established three independent ovine mammary epithelial cell lines which arose from primary cultures of ovine mammary epithelial cells by spontaneous immortalization. One of them, OMEC II, was characterised in greater detail. The cells grow on plastic dishes in medium containing 10% FCS without any requirement for additional growth factors or hormones. Immunofluorescence staining of this cell line showed expression of cytokeratin (46 kDa) and ZO-1, a tight-junction associated protein, but negative immunostaining for an anti-vimentin antibody. In confluent cell monolayers 'domes' because visible indicating the development of a polarised phenotype and the ability of directed secretion. When grown in collagen gels typical ducts with end-buds were observed. Treatment with lactogenic hormones increased the frequency of dome formation, but no expression of beta-lactoglobulin was found. To our knowledge this is the first report on an ovine mammary epithelial cell line.
Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Vienna, Austria.
M
M
Düchler
F
F
Schmoll
F
F
Pfneisl
G
G
Brem
K
K
Schellander
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D002461:Cell Line, Transformed; D016764:Cell Polarity; D003470:Culture Media; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007621:Karyotyping; D007633:Keratins / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology*; D008565:Membrane Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D008894:Milk Proteins / Q000096:biosynthesis / Q000235:genetics; D010641:Phenotype; D010750:Phosphoproteins / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology; D012756:Sheep / Q000033:anatomy & histology*; D062826:Zonula Occludens-1 Protein
D003470:Culture Media; D007328:Insulin; D008565:Membrane Proteins; D008894:Milk Proteins; D010750:Phosphoproteins; D062826:Zonula Occludens-1 Protein; D007633:Keratins; D011388:Prolactin; D006854:Hydrocortisone