Purification of extracellular catalase from Aspergillus niger.
The extracellular catalase (EC 1.11.1.6) produced by Aspergillus niger culture in 5-liter fermentor was isolated and purified by ion-exchange chromatography on DEAE Sepharose (fast flow) column, hydrophobic interaction on phenyl-Sepharose column and chromatofocusing on PBE 94 column. Some physico-chemical properties of two purified catalase forms were also determined (molecular weight, isolelectric point, polysaccharides contents, Km, and Ea).
Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
J
J
Rogalski
J
J
Fiedurek
A
A
Gromada
D001234:Aspergillus niger / Q000201:enzymology*; D002374:Catalase / Q000302:isolation & purification* / Q000378:metabolism; D002852:Chromatography, Ion Exchange / Q000379:methods*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D013053:Spectrophotometry
D002374:Catalase