The complete genome of Bacillus subtilis: from sequence annotation to data management and analysis.
The completion of the entire 4.2-Mb genome sequence of the gram-positive bacterium Bacillus subtilis has been a milestone for biological studies on this model organism. This paper describes bioinformatics work related to this joint European and Japanese project: methods and strategies for gene annotation and detection of sequencing errors, using an integrated cooperative computer environment (Imagene); construction of a specialized database for data management and a WWW server for data retrieval (SubtiList); DNA sequence analysis, yielding striking results on oligonucleotide bias, repeated sequences, and codon usage, all landmarks of evolutionary events shaping the B. subtilis genome.
Moszer
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Unité de Régulation de l'Expression Génétique, Institut Pasteur, Paris, France. moszer@pasteur.fr
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000235:genetics*; D003195:Computer Communication Networks; D016208:Databases, Factual; D016680:Genome, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA