Changes of isoenzymes of serum N-acetyl-beta-D-glucosaminidase in relation to different types of diabetes.
The activity of the isoenzymes of N-acetyl-beta-D-glucosaminidase (NAG, EC 3.2.1.30) is determined in the serum of insulin-dependent (IDDM) and non-insulin-dependent diabetics (NIDDM) with or without diabetic complications. The increase in total activity of serum NAG in diabetics is proportional to the A form activity (r = 0.976, p < 0.0001). The contribution of the A form activity (65.87 +/- 5.99%) to total NAG activity of IDDM and NIDDM diabetics with and without complications does not change considerably compared to the control group. The contribution of the B form activity depends on the state of metabolic monitoring and diabetic complications. A significantly lower activity of the serum B form was found in IDDM (p < 0.001) and NIDDM diabetics (p < 0.05) compared to the healthy individuals, as well as the higher activity ratios of the A/B forms. A decrease in serum B form is correlated with the occurrence and abundance of the intermediary I forms (r = 0.665). These changes are particularly significant in the individuals with the pronounced microangiopathy.
Faculty of Chemistry, University of Belgrade, Yugoslavia.
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L
Mandić
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D
Filipović
D000118:Acetylglucosaminidase / Q000097:blood*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000201:enzymology*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*
D007527:Isoenzymes; D000118:Acetylglucosaminidase