Serum affinity chromatography for the detection of IgG alloantibodies in a patient with high-titer IgM cold agglutinins.
OBJECTIVE
High-titer cold autoagglutinins (CA) interfere with the detection of alloantibodies against red-blood-cell antigens. The diagnostic procedure is extremely difficult, especially under pressure of time. It was the purpose of the study to find out whether quantitative IgG purification from serum or plasma by affinity chromatography facilitates the detection of IgG alloantibodies in the presence of high-titer IgM CA.


METHODS
Serum: IgM-chi CA, 0 degrees C titer = 65,000; five anti-IgG reactive alloantibodies. Affinity chromatography: HiTrap protein G 1-ml affinity column (Pharmacia Biotech, Uppsala, Sweden).


RESULTS
All IgG alloantibodies were detectable in the eluate after affinity chromatography without interference of IgM CA.


CONCLUSIONS
IgG alloantibodies in the presence of high-titer IgM CA can be easily and rapidly detected under routine conditions by quantitative IgG purification from serum or plasma by affinity chromatography.
Stahl
D
D
Institut für Immunologie, Ruprecht-Karls-universität, Heidelberg, Deutschland.
Kreft
H
H
Hack
H
H
Schraven
B
B
Roelcke
D
D
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology*; D001788:Blood Grouping and Crossmatching; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007075:Immunoglobulin M / Q000097:blood; D007518:Isoantibodies / Q000097:blood*; D008875:Middle Aged; D017186:Titrimetry
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007518:Isoantibodies