Mouse myoblasts can fuse and form a normal sarcomere in the absence of beta1 integrin expression.
Antibody perturbation experiments suggested that migration, terminal differentiation and fusion of myoblasts are dependent on beta1 integrin expression. In addition, several studies have postulated that beta1 integrins have a role in the formation of sarcomeres. In the present report we have analysed skeletal myogenesis in wild-type/beta1-null chimeric mice and beta1-null embryoid bodies. Trunk and limbs of beta1-null chimeric mice contained muscle tissue composed of normal and beta1-null myoblasts indicating that all myotomic sublineages can form, migrate to their peripheral targets and fuse in the absence of beta1 integrin expression. Pure populations of beta1-null myoblasts and satellite cells isolated from beta1-null chimeric embryos and chimeric newborn mice, respectively, were able to differentiate in vitro and to fuse into multinucleated myotubes. Quantitative and qualitative comparisons between normal and beta1-null myoblasts revealed no apparent difference in their capacity to terminally differentiate and fuse. Furthermore, beta1-null myotubes developed sarcomeres which were indistinguishable from wild-type controls. When normal and beta1-null ES cells were differentiated into embryoid bodies, they contained fully differentiated myotubes with normal sarcomeres and normal deposition of costameric components. However, formation of beta1-null myotubes was delayed and was less efficient in beta1-null embryoid bodies than in wild-type controls. High expression of alphav integrin subunit at the tips of normal as well as beta1-null myotubes indicated that the lack of beta1 integrins did not result in a significant redistribution of alphav-containing receptors.
Max Planck Institute for Biochemistry, Germany.
E
E
Hirsch
L
L
Lohikangas
D
D
Gullberg
S
S
Johansson
R
R
Fässler
D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation; D002459:Cell Fusion / Q000276:immunology / Q000502:physiology; D002465:Cell Movement; D002678:Chimera; D003598:Cytoskeletal Proteins / Q000378:metabolism; D049030:Dystroglycans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D039001:Integrin alpha Chains; D039564:Integrin alphaV; D019012:Integrin beta1 / Q000235:genetics / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008854:Microscopy, Electron; D018482:Muscle, Skeletal / Q000166:cytology* / Q000276:immunology*; D012518:Sarcomeres / Q000276:immunology* / Q000648:ultrastructure*; D013234:Stem Cells / Q000166:cytology / Q000276:immunology
D015703:Antigens, CD; D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D039001:Integrin alpha Chains; D039564:Integrin alphaV; D019012:Integrin beta1; D008562:Membrane Glycoproteins; C085725:integrin alpha7; D049030:Dystroglycans