Highly cationic anti-DNA antibodies in patients with lupus nephritis analyzed by two-dimensional electrophoresis and immunoblotting.
The relationship between chemical properties of anti-DNA antibodies (Abs) and lupus nephritis was investigated. The anti-DNA Abs in sera from systemic lupus erythematosus (SLE) patients were separated by two-dimensional electrophoresis (2-DE) and immunoblotting with goat anti-human IgG Abs. Highly cationic anti-DNA Abs were detected in deoxyribonuclease I (DNase I)-treated sera from patients with lupus nephritis (in 8 of 9 cases) but not in the sera from SLE patients without nephritis (in 0 of 9 cases), normal subjects, or patients with other renal diseases (in 0 of 7 cases). The mean titers of anti-dsDNA Abs in patients with lupus nephritis were not significantly different from those in SLE patients without nephritis. The highly cationic anti-DNA Abs in the sera disappeared after incubation with heparin-Sepharose. These results suggest that highly cationic anti-DNA Abs are specific for lupus nephritis and may be involved in development of lupus nephritis via the binding to glycosaminoglycans on the endothelial cell surface.
Kohro-Kawata
J
J
The Second Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Japan. ykawata@po.cc.yamaguchi-u.ac.jp
Wang
P
P
Kawata
Y
Y
Matsuzaki
M
M
Nakamura
K
K
D000293:Adolescent; D000328:Adult; D000974:Antibodies, Antinuclear / Q000097:blood*; D002412:Cations; D004247:DNA / Q000276:immunology*; D003850:Deoxyribonuclease I; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006493:Heparin; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D008181:Lupus Nephritis / Q000097:blood / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D012685:Sepharose
D000974:Antibodies, Antinuclear; D002412:Cations; D007074:Immunoglobulin G; D006493:Heparin; D004247:DNA; D012685:Sepharose; D003850:Deoxyribonuclease I