Effect of oviductal cell co-culture on cleavage and development of goat IVF embryos.
The present study was undertaken with the aim to study the role of isologous and heterologous (buffalo) oviductal cell to co-culture on in vitro development of goat embryos. The oocytes were collected by puncturing the goat ovaries obtained from slaughterhouse. The oocyte recovery rate per ovary was 3.0. The media used for oocyte maturation and embryo development was TCM-199 + 10 percent buffalo estrus serum. A total of 79.8 percent oocytes got matured out of 1056 oocytes. The oocytes were inseminated with epididymal buck spermatozoa capacitated in Brackett and Oliphant media. In group I without oviductal cells co-culture only 13.6 percent matured oocytes cleaved and 3.3 and 0.0 percent reached the morula and blastocyst stage. In group II and III having goat and buffalo oviductal cells the cleavage was 57.6 and 59.2 percent respectively. The percentage of morula, blastocyst and those embryos arrested between 2-16 cells were 26.3, 10.2, 63.5 and 26.6, 8.9 and 64.5 in goat and buffalo oviductal cell groups. The results indicated that the oviductal cell co-culture had a marked effect on cleavage and development of goat IVF embryos. Buffalo oviductal cells can be used well for goat embryo development.
Yadav
P S
PS
Central Institute for Research on Buffaloes, Hisar, India.
Saini
A
A
Kumar
A
A
Jain
G C
GC
D000818:Animals; D002020:Buffaloes / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005187:Fallopian Tubes / Q000166:cytology* / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D006041:Goats / Q000196:embryology* / Q000502:physiology; D008297:Male; D008858:Microscopy, Phase-Contrast / Q000662:veterinary; D009865:Oocytes / Q000502:physiology*; D011247:Pregnancy; D013084:Sperm-Ovum Interactions / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology