Evaluation of product analogues for purification of penicillinase by affinity chromatography.
Among different matrices prepared, ampicilloic acid-polymer matrix offered 86.7% adsorption, 95% elution and 82.4% overall recovery of penicillinase. The structure of both the side chain and penicilloic or cephalosporoic acid moieties contribute to the affinity interactions.
Kumar
K K
KK
Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune, India.
Deshpande
B S
BS
Ambedkar
S S
SS
Shewale
J G
JG
D000327:Adsorption; D001409:Bacillus cereus / Q000201:enzymology; D002511:Cephalosporins / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D005285:Fermentation; D008024:Ligands; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D010405:Penicillinase / Q000032:analysis / Q000302:isolation & purification*
D002511:Cephalosporins; D008024:Ligands; C008782:penicilloic acid; D010397:Penicillanic Acid; C030735:7-aminocephalosporanic acid; D010405:Penicillinase