Localized avidin/biotin derivatization of glassy carbon electrodes using SECM.
Different forms of the microreagent mode of SECM were used to attach biotin or make "clean" spots on micron-sized regions on the surface of a carbon electrode. In the direct-write mode, the SECM probe tip is used as an electrochemical "pen" depositing biotin in micron-sized lines on the carbon substrate as it is scanned across its surface. In the negative microreagent mode, the SECM probe tip is used as an electrochemical "eraser" cleaning of the surface attached molecules and leaving clean spots on the surface of a globally derivatized carbon surface. This type of simple micromodification of the surface of a carbon electrode will allow the fabrication of biosensors that can potentially be tailor-made for a variety of applications.
Department of Chemistry, Mississippi State University, Mississippi 39762, USA.
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D000469:Alkaline Phosphatase / Q000737:chemistry; D001360:Avidin / Q000031:analogs & derivatives* / Q000737:chemistry; D015374:Biosensing Techniques; D001710:Biotin / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D004566:Electrodes; D004800:Enzymes, Immobilized; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation; D013379:Substrate Specificity; D013499:Surface Properties
D004800:Enzymes, Immobilized; C036963:fluorescein isothiocyante avidin; D001360:Avidin; D001710:Biotin; D002244:Carbon; D000469:Alkaline Phosphatase; D016650:Fluorescein-5-isothiocyanate