Apoptosis in human gastric polyps and adenocarcinomas: a stereological analysis.
OBJECTIVE
The occurrence of apoptotic cells was analyzed in human normal gastric mucosa, polyps and adenocarcinomas.


METHODS
Histological classification was carried out on hematoxylin and eosin stained slides. The tissue was classified as follows: Normal gastric mucosa or adenomatous polyps. Gastric carcinoma specimens were histologically classified according to Lauren's classification into: A: Diffuse adenocarcinoma without metastasis, B: Diffuse adenocarcinoma with metastasis, C: Intestinal adenocarcinoma without metastasis, D: Intestinal adenocarcinoma with metastasis, E: Mixed adenocarcinoma without metastasis and mixed adenocarcinoma with metastasis. The counting of apoptotic cells was performed using the 40X objective with a calibrated eyepiece Weibel's multi-purpose M 42 stereological test system. Each group was evaluated stereologically, determining numeric density of apoptotic cells.


RESULTS
The results show the progressive and statistically significant increase of apoptotic numeric densities from normal gastric epithelium to adenomatous polyp and finally to cancer, which contained the highest number of apoptotic cells. Comparing gastric carcinoma with and without metastasis in intestinal and diffuse adenocarcinoma there was statistically significant difference. In these two groups, carcinomas with metastasis contained higher number of apoptotic cells than without metastasis. Gastric cancer according to numeric densities of apoptotic cells can be separated in tree statistically different groups: A: Intestinal type gastric cancer with metastasis (the highest number of apoptotic cells), B: Intestinal type gastric cancer without metastasis and diffuse gastric cancer with metastasis (medium number), C: Diffuse type gastric cancer without metastasis, mixed gastric cancer with and without metastasis (the lowest number of apoptotic cells).


CONCLUSIONS
These results suggest that numeric densities of apoptotic cells are associated with tumor progression in human gastric carcinogenesis and can be used as prognostic mark.
Mijic
A
A
Department of Surgery, University Hospital Sestre milosrdnice, Zagreb, Croatia. august.mijic@zg.tel.hr
Ferencic
Z
Z
Belicza
M
M
Fucic
A
A
Sevic
S R
SR
Sarcevic
B
B
Bekavac-Beslin
M
M
Grbesa
M
M
Golubic
D
D
Horzic
M
M
Doko
M
M
De Syo
D
D
Georgijevic
A
A
D000230:Adenocarcinoma / Q000473:pathology*; D000368:Aged; D017209:Apoptosis; D005260:Female; D005753:Gastric Mucosa / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D011127:Polyps / Q000473:pathology*; D011379:Prognosis; D013274:Stomach Neoplasms / Q000473:pathology*