Guanine nucleotides protect against kainate toxicity in an ex vivo chick retinal preparation.
Ex vivo preparations of chick neural retina have been successfully used in the assessment of excitotoxicity and in the evaluation of the protective effects of glutamate antagonists. Using a variation of this approach, and measuring the acute and delayed toxic effects of kainate (KA) in terms of lactate dehydrogenase release, we have shown that guanine nucleotides behave as effective neuroprotecting agents. The anti-excitotoxic potency of guanine nucleotides (in the case of GMP and GDPbetaS it is about 100 times lower than that of DNQX, a powerful kainate antagonist) correlates well with their ability to displace KA from retinal KA receptors.
Burgos
J S
JS
Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Barat
A
A
Souza
D O
DO
Ramírez
G
G
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane; D002642:Chick Embryo; D046508:Culture Techniques; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000494:pharmacology*; D007608:Kainic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000633:toxicity*; D011810:Quinoxalines / Q000494:pharmacology; D012160:Retina / Q000187:drug effects*
D018691:Excitatory Amino Acid Antagonists; D006150:Guanine Nucleotides; D011810:Quinoxalines; C056723:FG 9041; D007608:Kainic Acid