Amino acid sequence of glutathione S-transferase rGSTM5* from rat testis.
Glutathione S-transferase rGSTM5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. The protein was digested with Achromobacter protease I or endoproteinase Arg-C. The peptide fragments were isolated for electrospray MS and N-terminal peptide sequencing analyses. The primary amino acid sequence of rGSTM5* comprises 217 residues and has a calculated average molecular mass of 25495.3 Da. The result is identical to that obtained for rGSTM5* with liquid chromatography-MS from a mixture of rat testicular GSTs. Therefore, rGSTM5* has not been post-translationally modified.
Tam
M F
MF
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Republic of China.
Hsieh
C H
CH
Tsai
S P
SP
Tam
T C
TC
D000595:Amino Acid Sequence; D000818:Animals; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011499:Protein Processing, Post-Translational; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology*
D018076:DNA, Complementary; D007527:Isoenzymes; D005982:Glutathione Transferase