Intra-amygdala infusion of an end-capped antisense oligodeoxynucleotide to c-fos accelerates amygdala kindling.
The changes produced by repeated stimulation of the brain that result in the kindling effect remain unknown. It is known, however, that activation of immediate early genes including c-fos is a very early event in the development of kindling. Here we show that c-fos expression in the amygdala can be knocked-down by an end-capped antisense oligodeoxynucleotide (but not by vehicle, or control antisense oligodeoxynucleotides) and that this results in an acceleration of kindling. These results suggest that, at least in the amygdala, the expression of c-fos is associated with an attempt by the brain to retard the development of kindling.
Department of Pharmacology, Faculty of Medicine, Dalhousie University, Halifax, Canada.
B J
BJ
Chiasson
M G
MG
Hong
H A
HA
Robertson
D000661:Amphetamine / Q000494:pharmacology; D000679:Amygdala / Q000187:drug effects*; D000818:Animals; D016762:Genes, fos; D007263:Infusions, Parenteral; D007696:Kindling, Neurologic / Q000187:drug effects*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
D009419:Nerve Tissue Proteins; D016376:Oligonucleotides, Antisense; D016760:Proto-Oncogene Proteins c-fos; D000661:Amphetamine