Spinach CSP41, an mRNA-binding protein and ribonuclease, is homologous to nucleotide-sugar epimerases and hydroxysteroid dehydrogenases.
Spinach CSP41 is part of a protein complex that binds to the 3' untranslated region (UTR) of petD precursor-mRNA, a chloroplast gene encoding subunit IV of the cytochrome b6/f complex. CSP41 cleaves the 3'-UTR of petD mRNA within the stem-loop structure, suggesting a key role in the control of chloroplast mRNA stability. We discovered that CSP41 is homologous to nucleotide-sugar epimerases and hydroxysteroid dehydrogenases while seeking distant homologs of these enzymes with a hidden Markov model-based search of Genpept. This analysis identified Synechocystis ORF, Accession 1652543 as a homolog. Subsequent analyses show that spinach CSP41 and Arabidopsis thaliana 2765081 are homologous to the Synechocystis ORF. Information from the solved 3D structures of epimerases and dehydrogenases and our motif analysis of these enzymes is used to predict domains on CSP41 that are important in binding and metabolism of mRNA. Cyanobacteria are among the earliest life forms, indicating that the divergence from a common ancestor of nucleotide-sugar epimerases and an mRNA binding protein with ribonuclease activity was ancient.
0623B, 0114, University of California, San Diego, 9500 Gilman Drive, La Jolla, California, 92093-0623, USA.
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry; D000458:Cyanobacteria / Q000201:enzymology; D003573:Cytochrome b Group / Q000235:genetics; D045346:Cytochrome b6f Complex; D019992:Databases as Topic; D004722:Endoribonucleases / Q000737:chemistry*; D006913:Hydroxysteroid Dehydrogenases / Q000737:chemistry; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry; D017433:Protein Structure, Secondary; D012322:RNA Precursors / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry*; D019998:Racemases and Epimerases / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D012984:Software; D018724:Spinacia oleracea / Q000201:enzymology*
D001426:Bacterial Proteins; D003573:Cytochrome b Group; D010940:Plant Proteins; D012322:RNA Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C084072:petD protein, cytochrome b(6)f complex; D045346:Cytochrome b6f Complex; D006913:Hydroxysteroid Dehydrogenases; D004722:Endoribonucleases; C106104:endoribonuclease CSP41; D019998:Racemases and Epimerases