Immunohistochemical assessment of prostaglandin H-synthase in bovine endometrial biopsy samples collected throughout the oestrous cycle.
The study was designed to determine the distribution of prostaglandin H-synthase (PGS) also known as cyclooxygenase in specific uterine cell populations during the oestrous cycle. Endometrial biopsy samples were obtained from a total of 10 clinically healthy cows at days 1 (initiation of behavioural oestrus), 8, 15, and 19 of the oestrous cycle. All animals conceived after biopsy regimen. Data of semiquantitatively scored immunoreactivities were analysed using analyses of variance, t-tests for paired data and correlation analyses. Biotin-streptavidin-peroxidase immunostaining technique was employed to localise PGS. Specific staining was consistently present in endothelial cells of arteries but not capillaries and venules. A gradient of staining intensity was clearly apparent within the endometrium: surface epithelial cells and stromal cells located near the endometrial surface are consistently stained more intensely than glandular epithelial cells and stromal cells lying deeper in the endometrium. Days of oestrous cycle also influenced PGS immunoreactivities. Generally, higher immunoreactivities were recorded in surface epithelium, uterine glands and endometrial stromal cells at cycle days 1 and 19 as compared to cycle days 8 and 15. Minimal scoring values were mainly found at cycle day 8. The results of the present study suggest that the amount of bovine endometrial PGS varies considerably with the day of cycle in the above mentioned cell-type- and location-restricted manner. Therefore, the capacity of the bovine uterine mucosa for prostaglandin production may--amongst other factors--depend on the cycle-restricted availability of the respective enzyme systems.
Institute for Anatomy, Veterinary School, Hannover, Germany. aboos@anat.tiho-hannover.de
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Boos
D000818:Animals; D000911:Antibodies, Monoclonal; D001706:Biopsy / Q000662:veterinary; D002417:Cattle / Q000378:metabolism* / Q000502:physiology; D004717:Endometrium / Q000201:enzymology* / Q000502:physiology; D004847:Epithelial Cells / Q000276:immunology; D004971:Estrus / Q000378:metabolism* / Q000502:physiology; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008297:Male; D051379:Mice; D011247:Pregnancy; D011256:Pregnancy Outcome; D011374:Progesterone / Q000097:blood; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D011897:Random Allocation; D012756:Sheep; D017154:Stromal Cells / Q000276:immunology
D000911:Antibodies, Monoclonal; D011374:Progesterone; D011451:Prostaglandin-Endoperoxide Synthases