The flagellar switch genes fliM and fliN of Rhodobacter sphaeroides are contained in a large flagellar gene cluster.
In this work, the genes that encode the FliM and FliN proteins of Rhodobacter sphaeroides were characterized. These genes are part of a large flagellar gene cluster in which six additional open reading frames encoding products homologous to FliL, FliO, FliP, FliQ, FliR, and FlhB proteins from other bacteria were identified. The inactivation of the fliM gene gave a nonflagellate phenotype (Fla-), suggesting that FliM is required for flagellar assembly. Complementation analysis of this fliM mutant indicated that fliM and fliN transcription starts beyond the 5' end of fliK and terminates after fliN.
Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, UNAM, México 04510 D.F., Mexico.
N
N
García
A
A
Campos
A
A
Osorio
S
S
Poggio
B
B
González-Pedrajo
L
L
Camarena
G
G
Dreyfus
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics; D005407:Flagella / Q000235:genetics* / Q000502:physiology; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009068:Movement; D005810:Multigene Family; D010641:Phenotype; D012242:Rhodobacter sphaeroides / Q000235:genetics* / Q000502:physiology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D014158:Transcription, Genetic
D001426:Bacterial Proteins; C077389:FliN protein, Bacteria; C066821:FliM protein, Bacteria