Evaluation of the immunospecificity of the porin Om1 of Vibrio anguillarum serotype O1.
Three methods of immunoanalysis (immunoblot, ELISA and dot-blot) were used to evaluate the immunospecificity of the antiserum against the porin Om1 of Vibrio anguillarum serotype O1 with respect to all the serotypes of V. anguillarum, different Vibrio species and other Gram-negative genera. In the immunoblot analysis of the outer membrane proteins, this antiserum cross-reacted with the main outer membrane protein (MOMP) of all the Vibrio strains studied but not with other genera, except Plesiomonas shigelloides. However, when analyses were performed using whole cells as antigens (ELISA and dot-blot), the antiserum was more specific for V. anguillarum.
Department of Microbiology, University of Barcelona, Catalonia, Spain.
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Simón
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Jofre
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Blanch
D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006090:Gram-Negative Bacteria / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D018272:Porins / Q000032:analysis / Q000276:immunology*; D011817:Rabbits; D012703:Serotyping; D014733:Vibrio / Q000276:immunology*; D014735:Vibrio Infections / Q000175:diagnosis
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D018272:Porins