Intensity modulation of pseudocolor images.
Fura-2 is a popular real-time fluorescent indicator used to image intracellular, free calcium concentrations ([Ca2+]i) in living cells. One difficulty with fura-2-based digital imaging is how to best display the [Ca2+]i information that is acquired. Typically, only the [Ca2+]i as pseudocolor data are displayed, and the localization and intensity of the dye are not presented. This paper describes a technique that allows for the re-introduction of the intensity information. Recovery of the intensity information would de-emphasize regions with a poor signal-to-noise ratio and would more accurately report variations of sample morphology. A second advantage of this technique is that it requires no additional hardware since it is completely software based and can be run on all the major operating systems.
Hinman
L E
LE
University of Minnesota, Minneapolis, USA.
Sammak
P J
PJ
D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003116:Color / Q000592:standards*; D003118:Color Perception; D003626:Data Display; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D016257:Fura-2 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D051381:Rats
D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2