Continuous wave excitation two-photon fluorescence microscopy exemplified with the 647-nm ArKr laser line.
We report on efficient two-photon fluorescence imaging in beam scanning microscopy by exciting UV dyes at the 647-nm line of a continuous wave ArKr mixed gas laser. For a numerical aperture of 1.4 (oil), we used an illumination power of up to 210 mW at the sample. High-resolution images were obtained for DAPI-labelled cell nuclei within 4-60 s. Our method is a simple two-photon alternative to UV confocal imaging with the potential of becoming a very useful feature of laser scanning microscopy.
Booth
M J
MJ
High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Hell
S W
SW
D000818:Animals; D004247:DNA / Q000032:analysis; D004330:Drosophila; D005347:Fibroblasts; D007211:Indoles; D007834:Lasers; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation; D008856:Microscopy, Fluorescence / Q000295:instrumentation; D017785:Photons
D007211:Indoles; C007293:DAPI; D004247:DNA