CD1 presents antigens from a gram-negative bacterium, Haemophilus influenzae type B.
Human CD1 is a family of nonpolymorphic major histocompatibility complex class I-like molecules capable of presenting mycobacterial lipids, including lipoarabinomannan (LAM), to double-negative (DN; CD4(-) CD8(-)) as well as CD8(+) T cells. Structural similarities between LAM and the capsular polysaccharides of gram-negative bacteria led us to consider the latter as candidate CD1 ligands. We derived two CD1-restricted DN T-cell populations which proliferated to Haemophilus influenzae type b (Hib) antigen. One T-cell population also proliferated to proteinase K-treated Hib antigen, suggesting that it recognized a nonpeptide. Our work thus expands the universe of T cell antigens to include nonpeptides distinct from mycobacterial lipids and suggests a potential role for CD1-restricted T cells in immunity to Hib.
Fairhurst
R M
RM
Molecular Biology Institute, UCLA School of Medicine, Los Angeles, California 90095, USA.
Wang
C X
CX
Sieling
P A
PA
Modlin
R L
RL
Braun
J
J
D017951:Antigen Presentation / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D018949:Antigens, CD1 / Q000276:immunology*; D002478:Cells, Cultured; D019741:Haemophilus influenzae type b / Q000276:immunology*; D006801:Humans; D013601:T-Lymphocytes / Q000276:immunology*
D000942:Antigens, Bacterial; D018949:Antigens, CD1