Coexistence of s100beta and putative transmitter agents in vagal and glossopharyngeal sensory neurons of the rat.
The coexistence of S100beta with calcitonin gene-related peptide (CGRP), substance P (SP), somatostatin (SOM), nicotinamide adenosine dinucleotide phosphate-diaphorase (NADPH-d), and tyrosine hydroxylase (TH) was examined in the glossopharyngeal and vagal sensory ganglia. S100beta immunoreactive (-ir) neurons in the jugular and petrosal ganglia frequently colocalized CGRP- or SP-ir, whereas S100beta-ir neurons in the nodose ganglion infrequently contained CGRP- or SP-ir. No S100beta-ir neurons in the jugular and petrosal ganglia showed SOM-ir while the small number of SOM-ir neurons in the nodose ganglion colocalized S100beta-ir. Many neurons in the nodose ganglion colocalized S100beta-ir and NADPH-d activity, whereas S100beta-ir neurons in the jugular and nodose ganglia infrequently contained NADPH-d activity. S100beta- and TH-ir were frequently colocalized in nodose ganglion but not in petrosal or jugular ganglion neurons. These findings suggest relationships between S100beta and specific putative transmitters in functions of subpopulations of vagal and glossopharyngeal sensory neurons.
Second Department of Oral Anatomy, Okayama University Dental School, Okayama, Japan.
H
H
Ichikawa
C J
CJ
Helke
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D002135:Calcium-Binding Proteins / Q000032:analysis; D005455:Fluorescent Antibody Technique; D005930:Glossopharyngeal Nerve / Q000166:cytology*; D008297:Male; D009252:NADPH Dehydrogenase / Q000032:analysis; D009414:Nerve Growth Factors; D009475:Neurons, Afferent / Q000737:chemistry* / Q000201:enzymology; D009479:Neuropeptides / Q000032:analysis*; D009620:Nodose Ganglion / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins / Q000032:analysis*; D013004:Somatostatin / Q000032:analysis; D013373:Substance P / Q000032:analysis; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis; D014630:Vagus Nerve / Q000166:cytology*
D002135:Calcium-Binding Proteins; D009414:Nerve Growth Factors; D009479:Neuropeptides; D064568:S100 Calcium Binding Protein beta Subunit; D009418:S100 Proteins; D013373:Substance P; D013004:Somatostatin; D014446:Tyrosine 3-Monooxygenase; D009252:NADPH Dehydrogenase; D015740:Calcitonin Gene-Related Peptide