Light is associated with a 34 kDa ribonucleoprotein in spinach chloroplasts.
Chloroplast ribonucleoproteins (RNPs) encoded by the nuclear genome may play an important role in the post-transcriptional steps of light-induced chloroplast gene expression. To study the relationship between the synthesis of a 34 kDa ribonucleoprotein (p34) and light in spinach chloroplasts, a polyclonal antibody was raised against p34. Immunoblotting revealed that p34 synthesis was induced in a light dependent manner. In addition, levels of the large subunit of ribulose-1,5-bis phosphate carboxylase/oxygenase (Rubisco) encoded by the chloroplast genome fluctuated in relation to those of p34. These results suggest that the synthesis of p34 in response to light induces of chloroplast gene expression.
Laboratory of Biology, School of Liberal Arts and Sciences, Kitasato University, Sagamihara, Japan.
M
M
Kanekatsu
H
H
Sawada
D002736:Chloroplasts / Q000378:metabolism* / Q000528:radiation effects; D015151:Immunoblotting; D008027:Light; D010940:Plant Proteins / Q000096:biosynthesis* / Q000276:immunology; D012261:Ribonucleoproteins / Q000096:biosynthesis* / Q000276:immunology; D018724:Spinacia oleracea / Q000378:metabolism* / Q000528:radiation effects
D010940:Plant Proteins; D012261:Ribonucleoproteins