Delineation of human cord blood hematopoietic progenitor cell subsets with a unique monoclonal antibody AY19.
AY19, a unique mAb was used to better characterize the umbilical cord blood hematopoietic progenitor subpopulations. This mAb identifies an 85 kDa cell surface glycoprotein. In this present study we showed that AY19 mAb is reactive with 50-60% of the CD34+ cord blood cells. Extensive phenotypical studies revealed that AY19 mAb defines a novel CD34+ subset different from the ones defined by anti-CD90, anti-CD38, anti-CD33, anti-CD71, anti-CD19, anti-CD7 or anti-HLA-DR mAbs. We show that AY19 mAb reacts with both primitive and committed progenitors including myeloid and lymphoid progenitors. In addition, sorted CD34+high/AY19+ cells contain an increased number of CFU-GM and a decreased number of BFU-E compared with sorted CD34+high/AY19- cells. We show that AY19 mAb exhibits agonistic properties by inducing a significant increase in the size of CFU-GM colonies when added to serum-free liquid cultures of hematopoietic progenitors. This suggests that AY19 mAb identifies a cell surface receptor which may be involved in the regulation of hematopoietic cell proliferation.
INSERM UR448, IM3, Faculté de Médecine de Créteil, Université Paris XII, France.
L
L
Abadie-Fauconnier
E D
ED
Carosella
E
E
Gluckman
I G
IG
Mansur
C
C
Menier
L
L
Boumsell
A
A
Bensussan
D000911:Antibodies, Monoclonal / Q000276:immunology; D018952:Antigens, CD34; D002455:Cell Division; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000276:immunology; D011247:Pregnancy
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34