Topical local anaesthetics (EMLA) inhibit burn-induced plasma extravasation as measured by digital image colour analysis.
Amide local anaesthetics have previously been shown to reduce oedema and improve dermal perfusion following experimental burns. Previous studies have used invasive techniques for burn oedema quantification which do not allow continuous monitoring in the same animal. The present study used digital image colour analysis to investigate the effect of topical local anaesthetics on burn-induced extravasation of Evans blue albumin. A standardised full-thickness burn injury (1 x 1 cm) was induced in the abdominal skin of anaesthetised rats. The burn area was subsequently covered with 0.5 g of lidocaine-prilocaine cream 5% (25 mg of each in 1 g; EMLA, ASTRA, Sweden) or placebo cream during the first hour post-burn. One hour after the burn trauma, animals received Evans blue dye intravenously. Skin colour appearances were recorded by macrophotography before the burn and 5, 60. 65, 90, 120, 150, and 180 min post-burn. Colour slides were digitised and colour changes were analysed using the normalised red-green-blue (n-rgb) colour system. Results showed a significant inhibition of Evans blue extravasation between 60 and 180 min post-burn in EMLA-treated animals versus controls. Topical local anaesthetics are potent inhibitors of burn-induced plasma albumin extravasation, probably by direct action on vascular permeability and by inhibition of various steps of the pathophysiological response after burn injury.
Department of Orthopaedic Surgery, Sahlgrenska University Hospital, Mölndal, Sweden.
A
A
Jönsson
U
U
Mattsson
P
P
Tarnow
P
P
Nellgård
J
J
Cassuto
D000287:Administration, Topical; D000779:Anesthetics, Local / Q000008:administration & dosage*; D000818:Animals; D002056:Burns / Q000150:complications / Q000188:drug therapy* / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects*; D004396:Coloring Agents; D004487:Edema / Q000188:drug therapy / Q000209:etiology / Q000503:physiopathology; D005070:Evans Blue; D005119:Extravasation of Diagnostic and Therapeutic Materials; D005500:Follow-Up Studies; D007091:Image Processing, Computer-Assisted; D008012:Lidocaine / Q000008:administration & dosage*; D000077442:Lidocaine, Prilocaine Drug Combination; D008297:Male; D009824:Ointments; D010781:Photography; D011318:Prilocaine / Q000008:administration & dosage*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects / Q000293:injuries / Q000503:physiopathology*
D000779:Anesthetics, Local; D004396:Coloring Agents; D000077442:Lidocaine, Prilocaine Drug Combination; D009824:Ointments; D011318:Prilocaine; D005070:Evans Blue; D008012:Lidocaine