Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves.
The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-alpha protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-alpha proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected.
Shoji
K
K
Central Research Institute of Electric Power Industry, Chiba, Japan.
Iwasaki
T
T
Matsuki
R
R
Miyao
M
M
Yamamoto
N
N
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D015536:Down-Regulation / Q000235:genetics* / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant / Q000528:radiation effects*; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000528:radiation effects; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics* / Q000528:radiation effects; D010944:Plants / Q000235:genetics; D017422:Sequence Analysis, DNA; D028901:alpha Karyopherins
D018076:DNA, Complementary; C490630:KPNA2 protein, human; D009687:Nuclear Proteins; D028901:alpha Karyopherins