Enhanced expression of full-length TrkB receptors in young rat brain with hypoxic/ischemic injury.
Expression of TrkB receptors were studied in the cerebral cortex of normal rats and young rats with hypoxic/ischemic injury. TrkB expressing cells were present in the piriform cortex at birth and increased in number with age, and were finally present in the entire cerebral cortex. Density of TrkB cells reached adult levels at P30. They were morphologically regarded as pyramidal neurons and interneurons. Hypoxic/ischemic injury induced a tentative increase of full-length TrkB receptors. A novel appearance of TrkB expressing neurons and enhanced immunostaining on both cell soma and dendrites were observed in the peri-infarct areas and increased number of TrkB expressing neurons were detected in the contralateral cortex after carotid artery ligation. This increase was no longer evident after 48 h of hypoxia. Double immunostaining using antiserum against GFA or OX-42 revealed no co-localization of TrkB receptors and these molecules, while there were only slight co-localization of TrkB and calbindin-D28k molecules. The altered levels in responses to injury indicate that TrkB may play a crucial role in the early protective mechanism of the neurons with hypoxic/ischemic injury through ligands BDNF and/or NT-4/5.
Department of Pediatrics, Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu 520-2192, Japan.
S
S
Narumiya
M
M
Ohno
N
N
Tanaka
T
T
Yamano
M
M
Shimada
D000367:Age Factors; D000818:Animals; D000833:Animals, Suckling; D000918:Antibody Specificity; D001253:Astrocytes / Q000737:chemistry / Q000378:metabolism; D002545:Brain Ischemia / Q000378:metabolism*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D007839:Functional Laterality / Q000502:physiology; D002534:Hypoxia, Brain / Q000378:metabolism*; D007150:Immunohistochemistry; D008264:Macrophages / Q000737:chemistry / Q000378:metabolism; D017628:Microglia / Q000737:chemistry / Q000378:metabolism; D009474:Neurons / Q000737:chemistry / Q000378:metabolism; D018696:Neuroprotective Agents / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D018696:Neuroprotective Agents; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases