Cd2+ and Co2+ at micromolar concentrations mobilize intracellular Ca2+ via the generation of inositol 1,4,5-triphosphate in bovine chromaffin cells.
To understand the mechanisms underlying the Cd2+- and Co2+-induced intracellular Ca2+ mobilization, we measured the levels of inositol phosphates using bovine chromaffin cells. Studies using HPLC indicated that Cd2+, Co2+ and methacholine significantly increased the generation of 1,4,5-IP3. The results suggest that Cd2+ and Co2+ mobilize Ca2+ from IP3-sensitive Ca2+ stores, possibly through the presumptive Cd2+ receptor.
Department of Physiology, Kagoshima University, Faculty of Medicine, Kagoshima 890, Japan.
K
K
Yamagami
S
S
Nishimura
M
M
Sorimachi
D000311:Adrenal Glands / Q000166:cytology; D000818:Animals; D002104:Cadmium / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D003035:Cobalt / Q000494:pharmacology*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D009994:Osmolar Concentration
D002104:Cadmium; D003035:Cobalt; D015544:Inositol 1,4,5-Trisphosphate; D002118:Calcium