Determination of meropenem in serum by high-performance liquid chromatography with column switching.
A rapid, accurate and sensitive liquid chromatographic assay with on-line solid-phase extraction for determination of meropenem in serum is described. Sample was directly injected onto the extraction column for sample clean-up and extraction. Thereafter, using an on-line column-switching system the drug was quantitatively transferred and separated on a C18 analytical column. Ultraviolet absorption at 298 nm was used for detection. The assay was linear from 1 to 100 micrograms/ml. Recovery was 98.5%. Based on a 20-microliters sample volume (serum- water, 1:1, v/v), detection limit was 0.1 microgram/ml. An application of the method to study the pharmacokinetics of meropenem is given.
Institute of Biomedical Sciences, Faculty of Medicine, University of Ancona, Italy.
S
S
Bompadre
L
L
Ferrante
M
M
De Martinis
L
L
Leone
D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D000077731:Meropenem; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013845:Thienamycins / Q000097:blood* / Q000493:pharmacokinetics
D007202:Indicators and Reagents; D012997:Solvents; D013845:Thienamycins; D000077731:Meropenem