Nuclear structure--skeletal gene expression interrelationships.
Using the osteocalcin gene as a paradigm for bone tissue-specific transcription, evidence is presented for functional linkage of nuclear architecture with developmental and steroid hormone-responsive control. The involvement of chromatin structure, nucleosome organization and the nuclear matrix is evaluated within the context of integrating regulatory signals that activate and/or suppress transcription of the osteocalcin gene. Mechanisms are evaluated which direct the bone and hematopoietic-specific acute myelogenous leukemia/core binding factor (AML/CBF) transcription factors to nuclear matrix-associated subnuclear domains that are competent to support expression.
Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, 55 Lake Ave. North, Worcester, MA 01655, USA. Gary.Stein @banyan.ummed.edu
G S
GS
Stein
A J
AJ
van Wijnen
J L
JL
Stein
J B
JB
Lian
D000818:Animals; D001842:Bone and Bones / Q000502:physiology*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D002843:Chromatin / Q000187:drug effects / Q000648:ultrastructure; D050657:Core Binding Factor alpha Subunits / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000502:physiology; D015530:Nuclear Matrix / Q000502:physiology / Q000648:ultrastructure; D022022:Nuclear Pore / Q000502:physiology; D009707:Nucleosomes / Q000502:physiology; D010006:Osteoblasts / Q000502:physiology; D015675:Osteocalcin / Q000096:biosynthesis / Q000235:genetics*; D012516:Osteosarcoma; D011401:Promoter Regions, Genetic / Q000235:genetics; D017434:Protein Structure, Tertiary / Q000502:physiology; D011965:Receptors, Glucocorticoid / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology; D014157:Transcription Factors / Q000502:physiology; D014158:Transcription, Genetic / Q000187:drug effects / Q000502:physiology; D014807:Vitamin D / Q000494:pharmacology; D030181:Vitamin D Response Element / Q000502:physiology
D002843:Chromatin; D050657:Core Binding Factor alpha Subunits; D016328:NF-kappa B; D009707:Nucleosomes; D011965:Receptors, Glucocorticoid; D014157:Transcription Factors; D015675:Osteocalcin; D014807:Vitamin D