High-performance liquid chromatographic determination of furosemide in plasma and urine and its use in bioavailability studies.
A sensitive, selective and efficient reversed-phase high-performance liquid chromatographic (HPLC) method is reported for the determination of furosemide in human plasma and urine. The method has a sensitivity limit of 5 ng/ml in plasma, with acceptable within- and between-day reproducibilities and good linearity (r2>0.99) over a concentration range from 0.05 to 2.00 microg/ml. The one-step extract of furosemide and the internal standard (warfarin) from acidified plasma or urine was eluted through a muBondapak C18 column with a mobile phase composed of 0.01 M potassium dihydrogenphosphate and acetonitrile (62:38, v/v) adjusted to pH 3.0. Within-day coefficients of variation (C.V.s) ranged from 1.08 to 8.63% for plasma and from 2.52 to 3.10% for urine, whereas between-day C.V.s ranged from 4.25 to 10.77% for plasma and from 5.15 to 6.81% for urine at three different concentrations. The minimum quantifiable concentration of furosemide was determined to be 5 ng/ml. The HPLC method described has the capability of rapid and reproducible measurement of low levels of furosemide in small amounts of plasma and urine. This method was utilized in bioavailability/pharmacokinetic studies for the routine monitoring of furosemide levels in adults, children and neonate patients.
College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
H S
HS
Abou-Auda
M J
MJ
Al-Yamani
A M
AM
Morad
S A
SA
Bawazir
S Z
SZ
Khan
K I
KI
Al-Khamis
D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005665:Furosemide / Q000097:blood* / Q000493:pharmacokinetics / Q000652:urine*; D006801:Humans; D008297:Male; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D005665:Furosemide