Thin-layer chromatographic determination of monensin in feeds: screening method.
Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.
U. S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010, USA.
W W
WW
Landgraf
P F
PF
Ross
D000821:Animal Feed / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D008985:Monensin / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
D000935:Antifungal Agents; D007202:Indicators and Reagents; D008985:Monensin