Nucleolar localization of BRCA1 protein in human breast cancer.
Both a rabbit polyclonal BRCA1 antibody, K-18, and a mouse monoclonal BRCA1 antibody, AP 16, produced nucleolar epithelial cell staining on frozen tissue sections of human infiltrating mammary carcinomas. There was much less BRCA1 antibody staining in normal tissues; however, 2 intraductal tumors and a papilloma, found in proximity to the carcinomas showed considerable nucleolar immunoreactivity. MCF-7 cells fixed in methanol and immunostained with the same two antibodies also revealed nucleolar staining, however, after 4% paraformaldehyde fixation for three minutes, there were many fewer nuclei stained. Antigen retrieval methods on formalin-fixed, paraffin-embedded specimens produced tumor cell cytoplasmic staining with AP 16 and nuclear staining in both tumor and normal epithelial cells with another BRCA1 monoclonal antibody, SG 11.
Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
N
N
Tulchin
L
L
Ornstein
I J
IJ
Bleiweiss
M
M
Mendlowitz
E
E
Weinstein
S H
SH
Dikman
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal; D019313:BRCA1 Protein / Q000378:metabolism*; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000378:metabolism*; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D011817:Rabbits; D012016:Reference Values
D000911:Antibodies, Monoclonal; D019313:BRCA1 Protein