Thioltransferase activity of bovine lens glutathione S-transferase.
A Mu-class glutathione S-transferase purified to electrophoretic homogeneity from bovine lens displayed thioltransferase activity, catalysing the transthiolation reaction between GSH and hydroxyethyldisulphide. The thiol-transfer reaction is composed of two steps, the formation of GSSG occurring through the generation of an intermediate mixed disulphide between GSH and the target disulphide. Unlike glutaredoxin, which is only able to catalyse the second step of the transthiolation process, glutathioneS-transferase catalyses both steps of the reaction. Data are presented showing that bovine lens glutathione S-transferase and rat liver glutaredoxin, which was used as a thioltransferase enzyme model, can operate in synergy to catalyse the GSH-dependent reduction of hydroxyethyldisulphide.
Dal Monte
M
M
Dipartimento di Fisiologia e Biochimica, Università di Pisa, v. S. Maria, 55-56100 Pisa, Italy.
Cecconi
I
I
Buono
F
F
Vilardo
P G
PG
Del Corso
A
A
Mura
U
U
D000818:Animals; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D054477:Glutaredoxins; D005982:Glutathione Transferase / Q000302:isolation & purification / Q000378:metabolism*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D008099:Liver / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D011490:Protein Disulfide Reductase (Glutathione); D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D054477:Glutaredoxins; D007527:Isoenzymes; D011506:Proteins; D010088:Oxidoreductases; D011490:Protein Disulfide Reductase (Glutathione); D005982:Glutathione Transferase