An AFLP-based procedure for the efficient mapping of mutations and DNA probes in barley.
A strategy based upon AFLP markers for high-efficiency mapping of morphological mutations and DNA probes to linkage groups in barley is presented. First, 511 AFLP markers were placed on the linkage map derived from the cross Proctor x Nudinka. Second, loci controlling phenotypic traits were assigned to linkage groups by AFLP analysis, using F2 populations consisting of 30-50 mutant plants derived from crosses of the type "mutant x Proctor" and "mutant x Nudinka." To map DNA probes, 67 different wild-type barley lines were selected to generate F2 populations by crossing with Proctor and Nudinka. F2 plants that were polymorphic for a given RFLP fragment were classified into genotypic classes. Linkage of the RFLP polymorphism to 1 of the 511 AFLP loci was indicated by cosegregation. The use of the strategy is exemplified by the mapping of the mutation branched-5 to chromosome 2 and of the DNA probes Bkn2 and BM-7 to chromosomes 5 and 1, respectively. Map expansion and marker order in map regions with dense clustering of markers represented a particular problem. A discussion considering the effect of noncanonical recombinant products on these two parameters is provided.
Castiglioni
P
P
Max-Planck-Institut für Züchtungsforschung, 50829 Cologne, Germany.
Pozzi
C
C
Heun
M
M
Terzi
V
V
Müller
K J
KJ
Rohde
W
W
Salamini
F
F
D000483:Alleles; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D015342:DNA Probes; D017343:Genes, Plant; D008040:Genetic Linkage; D001467:Hordeum / Q000235:genetics*; D009154:Mutation; D010641:Phenotype; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length
D017931:DNA Primers; D015342:DNA Probes