Genomic organization, 5' flanking enhancer region, and chromosomal assignment of the cell cycle gene, p55Cdc.
p55Cdc is a mammalian homologue of a family of cell cycle proteins from widely divergent species, which contains WD repeats and has been implicated in cell cycle-regulated ubiquitin-mediated proteolysis. p55Cdc is highly expressed in proliferating but not in differentiated or growth-arrested cells. The expression, phosphorylation, and degradation of this protein have been shown to be cell cycle-regulated. We analyzed a 5.3-kb genomic region that contains the entire rat p55Cdc gene. The gene contains 10 exons ranging in size from 97 to 373 bp. The promoter region has a TAT box, four E-box consensus sequences, and potential binding sites for cell cycle-specific transcription factors. In transient transfection assays, a construct containing a 1000-nucleotide p55Cdc promoter region upstream of the chloramphenicol acetyltransferase reporter gene demonstrated a 12-fold increase in transcriptional activity. Finally, using radiation hybrid mapping techniques, we localized this gene to the human chromosome, 9q13-21.
Amgen Inc., Thousand Oaks, California 91320, USA.
J
J
Weinstein
J
J
Karim
D H
DH
Geschwind
S F
SF
Nelson
J
J
Krumm
K M
KM
Sakamoto
D000818:Animals; D064199:Cdc20 Proteins; D018797:Cell Cycle Proteins; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D018274:Electroporation; D004742:Enhancer Elements, Genetic; D005091:Exons; D018816:Genes, cdc; D015698:Genomic Library; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D051381:Rats; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D014407:Tumor Cells, Cultured
D064199:Cdc20 Proteins; C577494:Cdc20 protein, rat; D018797:Cell Cycle Proteins; D011506:Proteins; C577488:CDC20 protein, human; D015500:Chloramphenicol O-Acetyltransferase