Sequential identification of apoptotic and necrotic cells on individual cytospin preparations.
Here, we describe a simple methodology which allows the consecutive differentiation of apoptotic and necrotic cells on the same cytospin preparation of fresh and cultured cells. In this methodology, necrotic cells are initially identified on the cytospin preparations using trypan blue followed by the identification of in situ DNA fragmentation using the TUNEL assay. Identification of trypan blue and TUNEL positive cells in the same section permits the simultaneous assessment of necrotic and apoptotic cells.
Interstitial Lung Disease Unit, Occupational and Environmental Medicine, Imperial College at the National Heart and Lung Institute, London, UK. p.pantelidis@ic.ac.uk
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Santilli
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Knight
D017209:Apoptosis / Q000502:physiology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis / Q000378:metabolism; D018922:HL-60 Cells / Q000166:cytology / Q000473:pathology; D006801:Humans; D009336:Necrosis; D014343:Trypan Blue
D004273:DNA, Neoplasm; D004247:DNA; D014343:Trypan Blue