Identification of a large Myc-binding protein that contains RCC1-like repeats.
The protooncogene MYC plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis and has been implicated in a variety of human tumors. MYC and the closely related MYCN encode highly conserved nuclear phosphoproteins (Myc and NMyc) that apparently function as transcription factors in the cell. We have identified a large and highly conserved nuclear protein that interacts directly with the transcriptional activating domain of Myc (designated "protein associated with Myc" or Pam). Pam contains an extended amino acid sequence with similarities to a protein known as regulator of chromosome condensation (RCC1), which may play a role in the function of chromatin. The gene encoding Pam (PAM) is expressed in all of the human tissue examined, but expression is exceptionally abundant in brain and thymus. Pam binds specifically to Myc, but not NMyc. The region in Myc required for binding to Pam includes a domain that is essential for the function of Myc and that is frequently mutated in Burkitt's lymphomas. PAM is located within a 300-kb region on chromosome 13q22.
Guo
Q
Q
G. W. Hooper Foundation, University of California, San Francisco, CA 94143, USA. guoq@pop.nci.nih.gov
Xie
J
J
Dang
C V
CV
Liu
E T
ET
Bishop
J M
JM
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism; D002051:Burkitt Lymphoma / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002882:Chromosomes, Human, Pair 13; D004268:DNA-Binding Proteins / Q000737:chemistry*; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry*; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013950:Thymus Gland / Q000378:metabolism; D015533:Transcriptional Activation; D044767:Ubiquitin-Protein Ligases
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; C062521:RCC1 protein, human; D006899:Mixed Function Oxygenases; C114022:MYCBP2 protein, human; D044767:Ubiquitin-Protein Ligases