Minimally invasive detection of Piscirickettsia salmonis in cultivated salmonids via the PCR.
The attributes of the PCR allowed implementation of an assay for specific detection of Piscirickettsia salmonis from a few microliters of fish serum. This opens the way to less invasive modes of sampling for this microbial pathogen in salmonids.
Instituto de Biología, Universidad Católica de Valparaíso, Valparaíso, Chile.
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Marshall
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Heath
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Henríquez
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Orrego
D000818:Animals; D017756:Aquaculture; D016470:Bacteremia / Q000175:diagnosis / Q000662:veterinary; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000032:analysis / Q000302:isolation & purification; D005393:Fish Diseases / Q000175:diagnosis*; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D009132:Muscles / Q000382:microbiology; D009876:Operon / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012487:Salmonidae / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S