Altered adhesion efficiency and fibronectin content in fibroblasts from schizophrenic patients.
Cultured fibroblasts from the cutaneous tissue of 16 schizophrenic patients were compared with 16 control cultured fibroblasts from the healthy subjects. The fibroblasts from the schizophrenic patients showed a decreased adhesion efficiency within 30 min after plating compared to that of the control subjects. However, after 90 min, there was no significant difference between the groups, where more than 90% of the cells from both groups had adhesed to the plate. By immunohistochemistry and western blotting using the antibodies against integrin (VLA5), talin, vinculin, fodrin, vimentin, ankyrin, plectin, fibronectin, and focal adhesion kinase (FAK), there was no significant difference in localization and amount between the groups. The amount of fibronectin released into the medium in which the fibroblast had already kept confluency showed no significant difference between the groups. However, the fibronectin content in cell lysate within 48 h after plating was significantly lower in the schizophrenic group.
Department of Neuropsychiatry, Osaka University Medical School, Japan.
Y
Y
Miyamae
Y
Y
Nakamura
Y
Y
Kashiwagi
T
T
Tanaka
T
T
Kudo
M
M
Takeda
D000328:Adult; D000368:Aged; D002448:Cell Adhesion; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D005353:Fibronectins / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D012559:Schizophrenia / Q000503:physiopathology*; D013997:Time Factors
D005353:Fibronectins