Quantitative determination of semisynthetic cephamycins in human serum and urine by ion-exchange, reversed-phase and ion-pair chromatography.
High-performance liquid chromatographic methods have been developed for the determination of semisynthetic cephamycins: cefoxitin, cefmetazole and cefminox in human serum and urine samples. Serum samples spiked with each cephamycin were combined with an equal volume of methanol to remove proteins and, after centrifugation, and aliquot of the supernatant was analysed by ion-exchange, reversed-phase and ion-pair chromatography with hexadecyltrimethylammonium bromide as the ion-pairing agent. Urine samples were diluted, filtered and analysed by same chromatographic procedure. The cephamycins were detected by their ultraviolet absorbance (265-272 nm). It was possible to determine concentrations of cephamycins to 0.2 micrograms/ml in serum 2 micrograms/ml in urine samples with a good level of reproducibility and accuracy.
Departamento de Física, Química y E.G., Facultades de Biología y Veterinaria, Universidad de León, Spain.
J C
JC
García-Gonzalez
R
R
Méndez
J
J
Martín-Villacorta
D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002513:Cephamycins / Q000032:analysis* / Q000097:blood / Q000652:urine; D002852:Chromatography, Ion Exchange; D006801:Humans; D007202:Indicators and Reagents; D015203:Reproducibility of Results; D012996:Solutions; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; D002513:Cephamycins; D007202:Indicators and Reagents; D012996:Solutions; D012997:Solvents