Postischemic inflammation: a role for mast cells in intestine but not in skeletal muscle.
The objective of this study was to directly study a role for mast cells in ischemia-reperfusion (I/R)-induced mucosal and microvascular dysfunction. I/R was induced in the intestine and skeletal muscle (gastrocnemius and cremaster muscle) of wild-type mice and mast cell-deficient mice (W/Wv). Changes in mucosal permeability (blood-to-lumen clearance of 51Cr-EDTA), leukocyte infiltration (myeloperoxidase activity in the intestine and intravital microscopy in the cremaster muscle), and vascular permeability (tissue wet-to-dry weight ratio and FITC-albumin leakage) were measured as indexes of tissue dysfunction. In wild-type animals, intestinal I/R induced a significant increase in mucosal permeability, leukocyte infiltration, and vascular permeability. Mast cell-deficient animals were completely protected from I/R-induced mucosal dysfunction. However, skeletal muscle I/R induced a significant increase in leukocyte infiltration, FITC-albumin leakage, and edema formation to the same degree in both wild-type and mast cell-deficient animals. These data suggest that mast cells may be important mediators of I/R-induced mucosal and microvascular dysfunction in the intestine but not of microvascular dysfunction in skeletal muscle.
Kanwar
S
S
Immunology Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
Hickey
M J
MJ
Kubes
P
P
D000818:Animals; D002199:Capillary Permeability; D002860:Chromium Radioisotopes; D004492:Edetic Acid; D007249:Inflammation / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000502:physiology / Q000503:physiopathology*; D007511:Ischemia / Q000503:physiopathology*; D007583:Jejunum / Q000098:blood supply* / Q000502:physiology / Q000503:physiopathology*; D007962:Leukocytes / Q000502:physiology; D008407:Mast Cells / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008817:Mice, Mutant Strains; D018482:Muscle, Skeletal / Q000098:blood supply / Q000502:physiology / Q000503:physiopathology*; D009195:Peroxidase / Q000032:analysis; D015424:Reperfusion
D002860:Chromium Radioisotopes; D004492:Edetic Acid; D009195:Peroxidase