Subconjunctival injection of low doses of mitomycin C: effects on fibroblast proliferation.
We evaluated the effect of low doses of mitomycin C (MMC) in subconjunctival injection on fibroblast viability. Eighteen rabbits received subconjunctival injections: group 1 was injected with balanced salt solution (BSS), group 2 received MMC 0.025 mg, and group 3 MMC 0.005 mg. Tissue samples were taken 1 h after the injection (conjunctiva, sclera and cornea at 0 and 90 degrees) and cultured. The average scleral and conjunctival outgrowth in the MMC-treated groups was significantly inferior to the outgrowth of the samples treated with BSS. Subconjunctival injections of MMC (0. 025 and 0.005 mg) have an inhibitory effect on conjunctiva at 0 degrees and on scleral fibroblast populations.
Department of Ophthalmology, Miguel Servet Hospital, Zaragoza, Spain. sope.pini@sendanet.es
I
I
Pinilla
J M
JM
Larrosa
V
V
Polo
F M
FM
Honrubia
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000008:administration & dosage*; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects; D003228:Conjunctiva / Q000187:drug effects / Q000473:pathology*; D003315:Cornea / Q000187:drug effects / Q000473:pathology; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D005500:Follow-Up Studies; D007267:Injections; D016685:Mitomycin / Q000008:administration & dosage*; D011817:Rabbits; D012590:Sclera / Q000187:drug effects / Q000473:pathology
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin