Evidence that porcine native 5-HT3 receptors do not contain nicotinic acetylcholine receptor subunits.
The present investigation utilised monoclonal antibodies directed against subunits of the nicotinic acetylcholine receptor in immunoblot and immunoprecipitation studies, which failed to demonstrate that the native 5-hydroxytryptamine3 (5-HT3) receptor complex purified from porcine brain contains the alpha1, alpha3, alpha4, alpha5, alpha7 or beta2 subunits of the nicotinic acetylcholine receptor.
Fletcher
S
S
Department of Pharmacology, The Medical School, University of Birmingham, Edgbaston, UK. S.Fletcher@bham.ac.uk
Lindstrom
J M
JM
McKernan
R M
RM
Barnes
N M
NM
D000818:Animals; D001923:Brain Chemistry; D015151:Immunoblotting; D011978:Receptors, Nicotinic / Q000737:chemistry*; D011985:Receptors, Serotonin / Q000737:chemistry*; D044406:Receptors, Serotonin, 5-HT3; D013552:Swine
D011978:Receptors, Nicotinic; D011985:Receptors, Serotonin; D044406:Receptors, Serotonin, 5-HT3