Two-dimensional chromatography in the analysis of complex glycans from transferrin.
Oligosaccharides released from human transferrin have been derivatized with 2-aminoacridone (2-AMAC) prior to analysis by either reversed- or normal-phase high-performance liquid chromatography. These separation methods are complementary and allow distinction between isomeric mono- and disialylated oligosaccharides, which terminate with Sia alpha 2-6Gal or Sia alpha 2-3Gal at the nonreducing end. Collected fractions of 2-AMAC-derivatized glycans were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, before and after desialylation.
SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UK.
J
J
Charlwood
H
H
Birrell
D
D
Tolson
P
P
Camilleri
D000609:Aminoacridines / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005456:Fluorescent Dyes / Q000737:chemistry; D005643:Fucose / Q000737:chemistry; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014168:Transferrin / Q000737:chemistry*
D000609:Aminoacridines; D005456:Fluorescent Dyes; D011134:Polysaccharides; D014168:Transferrin; C072515:2-aminoacridone; D005643:Fucose; D019158:N-Acetylneuraminic Acid