Effects of gamma-glutamylcysteine ethyl ester on heart mitochondrial creatine kinase activity: involvement of sulfhydryl groups.
To study the mechanism of the protective effect of gamma-glutamylcysteine ethyl ester, mitochondrial creatine kinase activity of rat heart was measured. Gamma-glutamylcysteine ethyl ester had a protective effect against the depression of creatine kinase activity induced by xanthine + xanthine oxidase or hydrogen peroxide. Gamma-glutamylcysteine ethyl ester also prevented the depression of creatine kinase activity induced by N-ethylmaleimide. It is suggested that the protective effect of gamma-glutamylcysteine ethyl ester is related to oxygen free radicals or to reduction of the sulfhydryl groups of the enzyme which were previously oxidized by oxygen free radicals.
Hayashi
H
H
Photon Medical Research Center, Hamamatsu University School of Medicine, Japan. hide289@hama-med.ac.jp
Iimuro
M
M
Matsumoto
Y
Y
Kaneko
M
M
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D004151:Dipeptides / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000502:physiology*; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D004151:Dipeptides; D004791:Enzyme Inhibitors; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D013481:Superoxides; C067744:N-gamma-glutamylcysteine ethyl ester; D003402:Creatine Kinase; D005033:Ethylmaleimide