Capillary electrophoretic analysis of ginseng polypeptide.
A ginseng polypeptide (GPP) found in ginseng roots and its modified peptides were tested by capillary zone electrophoresis (CZE) under acidic as well as basic conditions. Modified peptides were synthesized for three purposes: (i) to analyze their functions in the first three acidic amino acid residues, (ii) to analyze their functions in three sequenced glycines, and (iii) to analyze the length of side chains in acidic amino acids. The roles of glycines, acidic amino acids and amino acid side chains in the binding of Mg2+ were studied at pH values less than 7.0. The migration times of GPP varied with the pH of various electrophoresis buffers, and electrophoresis patterns were significantly changed between pH 7.0-7.5. Based on the electrophoretic analysis, it was concluded that the binding mechanisms for Mg2+ or conformations of GPP changed between low pH and high pH.
Kajiwara
H
H
Department of Biotechnology, National Institute of Agrobiological Resources, Kannondai, Tsukuba, Japan. kajiwara@abr.affrc.go.jp
Hemmings
A M
AM
D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D015394:Molecular Structure; D005894:Panax / Q000737:chemistry*; D010455:Peptides / Q000032:analysis; D010710:Phosphates; D010940:Plant Proteins / Q000032:analysis*; D010946:Plants, Medicinal; D019346:Sodium Acetate; D014325:Tromethamine
D002021:Buffers; D010455:Peptides; D010710:Phosphates; D010940:Plant Proteins; C104839:ginseng pentadecapeptide, Panax ginseng; D014325:Tromethamine; D019346:Sodium Acetate; C018279:sodium phosphate