Rb1 gene expression in B-cell lymphocytic leukaemia cases with deletion in the 13q14 region.
Recent studies have been carried out to delineate further the role of the Rb1 gene in B-cell chronic lymphocytic leukaemia (B-CLL). The suggested role of the Rb1 gene in this disease was based on cytogenetic data. CLL patients (40 in toto) were examined using cytogenetic and molecular biological methods. R-banding analysis of metaphase chromosomes revealed aberrations in only seven cases containing either the Rb1 gene or a chromosome 13 monosomy. No evident differences were found by RT-PCR analysis of Rb1 gene expression. The amounts of the RT-PCR products obtained appeared to be approximately equal in all cases, and was independent of the clinical stage, immunophenotypes and LPS or TPA stimulation.
Antosz
H
H
Department of Human Genetics, Medical Academy of Lublin, Poland.
Kitlińska
J
J
Kwiatkowska-Drabik
B
B
Koczkodaj
D
D
Wach
M
M
Antosz
M
M
Rudzki
S
S
Wojcierowski
J
J
D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000235:genetics; D002871:Chromosome Banding; D002872:Chromosome Deletion; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D005260:Female; D015973:Gene Expression Regulation, Leukemic / Q000235:genetics*; D016161:Genes, Retinoblastoma / Q000235:genetics*; D006801:Humans; D016130:Immunophenotyping; D007621:Karyotyping; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics*; D008214:Lymphocytes; D008297:Male; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000097:blood; D012334:RNA, Neoplasm / Q000097:blood
D012333:RNA, Messenger; D012334:RNA, Neoplasm