Interleukin-1 beta and interleukin-1 receptor antagonist levels in cerebrospinal fluid of aseptic meningitis patients.
The inflammatory process in aseptic meningitis is generally mild and self-limited. To evaluate control mechanisms underlying this process, we determined interleukin (IL)-1 beta and IL-1 receptor antagonist (IL-1ra) concentrations, using enzyme linked immunosorbent assay, in cerebrospinal fluid from patients with aseptic meningitis (n = 55, median age [range]; 5.0 [1-15] years). We cross-sectionally analyzed the relationship of IL-1 beta and IL-1ra levels to leucocyte count in terms of interval between clinical onset and lumbar puncture. IL-1 beta levels were highest in the cerebrospinal fluid obtained between 12 and 24 hours after onset (n = 23, median: 2.30 pg/ml). The IL-1 beta level was significantly correlated with leucocyte count. On the other hand, IL-1ra level tended to increase over a longer interval, reaching a plateau between 24 and 36 hours after onset (n = 13, median: 5190 pg/ml). The molar ratio of IL-1ra to IL-1 beta was thus lowest between 12 and 24 hours after onset (median: 2341), which coincided with the peak increase in leucocyte count. The results are compatible with the idea that IL-1ra should be important for the regulation of IL-1 beta activity associated with leucocyte migration in aseptic meningitis.
Yokoyama
T
T
Department of Pediatrics, Okayama University Medical School, Japan.
Oda
M
M
Seino
Y
Y
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D005260:Female; D005947:Glucose / Q000134:cerebrospinal fluid; D016179:Granulocyte Colony-Stimulating Factor / Q000134:cerebrospinal fluid; D006801:Humans; D007223:Infant; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1 / Q000134:cerebrospinal fluid*; D016209:Interleukin-8 / Q000134:cerebrospinal fluid; D007958:Leukocyte Count; D008137:Longitudinal Studies; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid* / Q000453:epidemiology; D009504:Neutrophils / Q000166:cytology; D011506:Proteins / Q000032:analysis; D012795:Sialoglycoproteins / Q000134:cerebrospinal fluid*; D013129:Spinal Puncture; D018709:Statistics, Nonparametric; D013997:Time Factors
C507763:IL1RN protein, human; D053590:Interleukin 1 Receptor Antagonist Protein; D007375:Interleukin-1; D016209:Interleukin-8; D011506:Proteins; D012795:Sialoglycoproteins; D016179:Granulocyte Colony-Stimulating Factor; D005947:Glucose