Cloning of sgk serine-threonine protein kinase from shark rectal gland – a gene induced by hypertonicity and secretagogues.
Recently, the cell-volume-regulated serine-threonine protein kinase h-sgk was cloned from a human hepatoma cell line. The sgk gene was shown to be induced by cell shrinkage in many different mammalian cell lines. In this study, two highly conserved serine-threonine protein kinases, sgk-1 and sgk-2, were cloned from rectal gland tissue of the spiny dogfish (Squalus acanthias). Both kinases showed a distinct pattern of tissue specificity, with high expression levels in kidney, intestine, liver and heart. In rectal gland slices sgk-1 transcription was induced by exposure to hypertonic solution, reduction of the extracellular urea concentration, and addition of the secretagogues vasoactive intestinal polypeptide (VIP) and carbachol. The shark sgk-1 serine-threonine protein kinase may therefore provide a link between cell volume, Cl–secretion and protein phosphorylation state in shark rectal gland cells.
Department of Physiology I, University of Tübingen, Gmelinstr. 5, D-72076 Tübingen, Germany.
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Waldegger
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Barth
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Forrest
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Greger
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Lang
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004284:Dogfish / Q000033:anatomy & histology*; D005786:Gene Expression Regulation; D006801:Humans; D006982:Hypertonic Solutions; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D012491:Salt Gland / Q000201:enzymology* / Q000502:physiology*; D016415:Sequence Alignment; D000099184:Serum-Glucocorticoid Regulated Kinases
D006982:Hypertonic Solutions; D017874:Immediate-Early Proteins; D017346:Protein Serine-Threonine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases