Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.
Plasmid reporter vectors have been constructed which respond to activation of LuxR and its homologues LasR and RhlR (VsmR) by N-acyl homoserine lactones (AHLs). The expression of luxCDABE from transcriptional fusions to PluxI, PlasI and PrhlI respectively, occurs in the presence of activating AHLs. A profile of structure/activity relationships is seen where the natural ligand is most potent. The characterisation of individual LuxR homologue/AHL combinations allows a comprehensive evaluation of quorum sensing signals from a test organism.
Food Microbiology Section, School of Biological Sciences, University of Nottingham, Leics, UK.
M K
MK
Winson
S
S
Swift
L
L
Fish
J P
JP
Throup
F
F
Jørgensen
S R
SR
Chhabra
B W
BW
Bycroft
P
P
Williams
G S
GS
Stewart
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D003227:Conjugation, Genetic; D004268:DNA-Binding Proteins / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D008163:Luminescent Measurements; D010957:Plasmids / Q000235:genetics*; D012097:Repressor Proteins / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics*; D013329:Structure-Activity Relationship; D015534:Trans-Activators / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D012097:Repressor Proteins; C086515:RhlR protein, Pseudomonas aeruginosa; D015534:Trans-Activators; C064778:LuxR autoinducer binding proteins; C088386:homoserine lactone; D015107:4-Butyrolactone