Biosensor characterization of antigenic site A of foot-and-mouth disease virus presented in different vector systems.
The region 141-160 of the VP1 protein of foot-and-mouth disease virus known as site A is an immunodominant region that has been extensively studied for the purpose of developing a synthetic vaccine. In the present study, site A of foot-and-mouth disease virus was inserted in three different loops of the maltose-binding protein and its antigenicity was compared with site A presented as a conjugated synthetic peptide or inserted in beta-galactosidase. The affinity of antibodies elicited against the site A synthetic peptide was also compared with that of antibodies raised against the site A inserted within the two carrier proteins. Using biosensor technology it was possible to estimate the concentration of site A antibodies present in the various antisera and to show that site A fused to maltose-binding protein was a slightly better mimic of the epitope present in the virus particle than the synthetic peptide or the beta-galactosidase recombinant construct.
Immunochemistry Laboratory (UPR9021), Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
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Benito
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Van Regenmortel
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D000915:Antibody Affinity; D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology*; D005537:Aphthovirus / Q000276:immunology*; D015374:Biosensing Techniques; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D036022:Capsid Proteins; D002352:Carrier Proteins / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D007114:Immunization; D016056:Immunodominant Epitopes / Q000276:immunology; D058619:Maltose-Binding Proteins; D018716:Molecular Mimicry; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000235:genetics / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D014614:Vaccines, Synthetic / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D002352:Carrier Proteins; D016056:Immunodominant Epitopes; D058619:Maltose-Binding Proteins; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D001616:beta-Galactosidase