Expression of beta-adrenergic receptors in the rat uterus: effects of puberty and oestrogen treatment during prepubertal development.
The expression of beta-adrenoceptors in the rat uterus has been analysed during the peripubertal transition and following acute and chronic oestradiol treatment during prepubertal development. The distribution and density of beta-adrenoceptors was assessed autoradiographically on cryostat tissue sections using [3H]-dihydroalprenolol ([3H]-DHA). Binding sites were localised in all ages and experimental situations examined and showed the following intensity of labelling: endometrial epithelium > longitudinal muscle layer > circular myometrial layer > endometrial stroma. Competition experiments with the selective antagonists ICI 118,551 and atenolol, showed that most of the beta-adrenoceptors in the uterus belong to the beta(2) receptor subclass. In prepubertal animals, the density of [3H]-DHA binding sites was extremely low. Following puberty the density of binding sites showed a generalised increase. Acute administration of oestradiol at the end of the prepubertal period provoked an increase in the density of [3H]-DHA binding sites in all uterine regions, but the levels of labelling were lower than in peripubertal animals at proestrus and oestrus. Following chronic oestrogen treatment during postnatal development, oestradiol increased further the density of [3H]-DHA binding sites. Results are discussed considering both the endocrine and neural changes accompanying puberty and oestradiol treatment.
División Biologia Celular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
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Brauer
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Burnstocks
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D004082:Dihydroalprenolol / Q000494:pharmacology; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D004967:Estrogens / Q000494:pharmacology*; D004971:Estrus / Q000502:physiology; D005260:Female; D009215:Myometrium / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011869:Radioligand Assay; D051381:Rats; D017208:Rats, Wistar; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis*; D012741:Sexual Maturation / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D014599:Uterus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure
D000319:Adrenergic beta-Antagonists; D004967:Estrogens; D011943:Receptors, Adrenergic, beta; D004082:Dihydroalprenolol