Determination of transforming growth factor-beta 1 (TGF-beta 1) and insulin-like growth factor (IGF-1) in bovine colostrum samples.
The major growth factors in bovine colostrum are transforming growth factor-beta s (TGF-beta 1 and TGF-beta 2) and insulin-like growth factors (IGF-1 and IGF-2). Recently, TGF-beta 2 content of bovine colostrum was measured using a TGF-beta 2 specific ELISA (1) and now we have validated ELISAs for for bovine TGF-beta 1 and IGF-1. The concentrations of IGF-1 and TGF-beta 1 in the first milking after calving were 248-1850 ng/ml and 12.4-42.6 ng/ml, respectively, and they declined in correlation with total protein concentration to 27.0-101 ng/ml (IGF-1) and 0.80-3.49 ng/ml(TGF-beta 1) by the fifth milkings. The amount of TGF-beta 1 was on average 5.3 +/- 1.4% of that of TGF-beta 2 and there is a high correlation (r = 0.966) between the concentrations of these growth factors in the same samples. No free TGF-beta 1 form of could be detected.
Department of Biochemistry and Food Chemistry, University of Turku, Finland.
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Pakkanen
D000818:Animals; D002417:Cattle; D003126:Colostrum / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007334:Insulin-Like Growth Factor I / Q000032:analysis*; D008892:Milk / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016212:Transforming Growth Factor beta / Q000032:analysis*
D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I