Effect of prooxidants on mitochondrial permeability transition and cell death in Ehrlich ascites tumour cells.
Ca2+ retention in mitochondria, opening of the Cysclosporin A- sensitive permeability transition pore and cell death were studied in Ehrlich ascites tumour cells in the presence of different prooxidants. Low concentrations (1-20 microM) of the prooxidants (menadione, cumenehydroperoxide, t-butylhydroperoxide) induced pore-opening in permeabilized cells at threshold Ca2+ load. Incubation of cells with low concentrations of prooxidants was able to induce cell cycle disturbance and cell death. Under the prooxidant effect, mitochondrial membrane potential drop and Ca2+ retention decrease in mitochondria were found to precede death of Ehrlich ascites tumour cells.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Puschino, Moscow Region, Russia. teplova@venus.iteb.serpukhov.su
V V
VV
Teplova
A A
AA
Kudrjavtsev
I V
IV
Odinokova
Y V
YV
Evtodienko
N E
NE
Saris
D000818:Animals; D001555:Benzene Derivatives / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002286:Carcinoma, Ehrlich Tumor; D016923:Cell Death; D002470:Cell Survival; D007425:Intracellular Membranes / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D010539:Permeability; D010545:Peroxides / Q000494:pharmacology; D014407:Tumor Cells, Cultured; D014812:Vitamin K / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D001555:Benzene Derivatives; D016877:Oxidants; D010545:Peroxides; D014812:Vitamin K; D020122:tert-Butylhydroperoxide; C007164:cumene hydroperoxide; D002118:Calcium