slimb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development.
In the Drosophila leg disc, wingless (wg) and decapentaplegic (dpp) are expressed in a ventral-anterior and dorsal-anterior stripe of cells, respectively. This pattern of expression is essential for proper limb development. While the Hedgehog (Hh) pathway regulates dpp and wg expression in the anterior-posterior (A/P) axis, mechanisms specifying their expression in the dorsal-ventral (D/V) axis are not well understood. We present evidence that slimb mutant clones in the disc deregulate wg and dpp expression in the D/V axis. This suggests for the first time that their expression in the D/V axis is actively regulated during imaginal disc development. Furthermore, slimb is unique in that it also deregulates wg and dpp in the A/P axis. The misexpression phenotypes of slimb- clones indicate that the regulation of wg and dpp expression is coordinated in both axes, and that slimb plays an essential role in integrating A/P and D/V signals for proper patterning during development. Our genetic analysis further reveals that slimb intersects the A/P pathway upstream of smoothened (smo).
Howard Hughes Medical Institute, Department of Genetics, Yale University Medical School, New Haven, CT 06535, USA.
N A
NA
Theodosiou
S
S
Zhang
W Y
WY
Wang
T
T
Xu
D000595:Amino Acid Sequence; D000818:Animals; D019521:Body Patterning / Q000235:genetics*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology; D003001:Cloning, Molecular; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development*; D029721:Drosophila Proteins; D005121:Extremities / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009030:Mosaicism; D009154:Mutation; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000235:genetics; D044767:Ubiquitin-Protein Ligases; D051155:Wnt1 Protein
D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D011518:Proto-Oncogene Proteins; D051155:Wnt1 Protein; C064196:dpp protein, Drosophila; C110233:slmb protein, Drosophila; C074622:wg protein, Drosophila; D044767:Ubiquitin-Protein Ligases