Digestion of 125I-labelled plasmin-derived fibrin degradation products by neutrophil lysosomal enzymes.
The cellular components of the blood, which become associated with fibrin through specific cellular adhesive processes, play a significant role in the breakdown of fibrin. Fibrinolysis by neutrophil elastase and cathepsin G occurs in a manner distinct from that produced by plasmin. This study demonstrates that neutrophil lysosomal enzyme activity further degrades the end products of plasmic fibrin degradation into low-molecular-weight material, followed by reassembly of higher-molecular-weight products in a process dependent on calcium and factor XIII. Although one of the reformed products has a similar molecular weight to D-dimer and is recognized by a monoclonal antibody raised against D-dimer, its isoelectric point indicates it to be distinctly different from plasmin-derived D-dimer. Processing of the end products of plasmic fibrin degradation by neutrophils may have the potential for modulating the immune response as well as compromising the predictive value of tests measuring D-dimer.
Adams
S A
SA
MRC/UCT Liver Research Centre, Department of Medicine, University of Cape Town, South Africa.
Kelly
S L
SL
Kirsch
R E
RE
Shephard
E G
EG
D000911:Antibodies, Monoclonal / Q000032:analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005337:Fibrin / Q000737:chemistry; D005338:Fibrin Fibrinogen Degradation Products / Q000737:chemistry* / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D005341:Fibrinolysin / Q000378:metabolism*; D006801:Humans; D007457:Iodine Radioisotopes; D007526:Isoelectric Point; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D008970:Molecular Weight; D009504:Neutrophils / Q000201:enzymology* / Q000648:ultrastructure*; D019163:Reducing Agents / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate; D013095:Spermidine / Q000494:pharmacology; D013096:Spermine / Q000494:pharmacology; D013997:Time Factors
D000911:Antibodies, Monoclonal; D004791:Enzyme Inhibitors; D005338:Fibrin Fibrinogen Degradation Products; D007457:Iodine Radioisotopes; D019163:Reducing Agents; C036309:fibrin fragment D; D013096:Spermine; D012967:Sodium Dodecyl Sulfate; D005337:Fibrin; D005341:Fibrinolysin; D013095:Spermidine