Characterization of whole genome radiation hybrid mapping resources for non-mammalian vertebrates.
Radiation hybrid panels are already available for genome mapping in human and mouse. In this study we have used two model organisms (chicken and zebrafish) to show that hybrid panels that contain a full complement of the donor genome can be generated by fusion to hamster cells. The quality of the resulting hybrids has been assessed using PCR and FISH. We confirmed the utility of our panels by establishing the percentage of donor DNA present in the hybrids. Our hybrid resources will allow inexpensive gene mapping and we expect that this technology can be transferred to many other species. Such successes are providing the basis for a new era of mapping tools, in the form of whole genome radiation hybrid panels, and are opening new possibilities for systematic genome analysis in the animal genetics community.
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK. cheni_kwok-1@sbphrd.com
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Kwok
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Korn
M E
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Davis
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Burt
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Critcher
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McCarthy
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Paw
L I
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Zon
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Goodfellow
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Schmitt
D000818:Animals; D002460:Cell Line; D002645:Chickens; D002874:Chromosome Mapping; D006224:Cricetinae; D006822:Hybrid Cells / Q000528:radiation effects*; D017404:In Situ Hybridization, Fluorescence; D016133:Polymerase Chain Reaction; D015027:Zebrafish