An evaluation of lectin-mediated magnetic bead cell sorting for the targeted separation of enteric bacteria.
Lectin-labelled magnetic beads were assessed and compared with antisera as an alternative approach for the targeted separation and isolation of enteric bacteria. Of the 16 lectins tested against a range of bacterial species, concanavalin A (conA) showed the greatest potential. Agglutination of bacterial cells in suspension using conA and methods for effective labelling of the magnetic beads with the lectin were optimized. ConA-labelled magnetic beads were compared with antibody-labelled beads for recovery of bacterial cells from pure or mixed laboratory cultures and from natural populations in river water. Recovered cell populations were free from environmental impurities and a high percentage of the culturable cells was extracted. Specific cell recovery was found to be variable, but the use of lectins offers some promise as an alternative cell discriminator.
School of Biological Sciences, University of Liverpool, UK.
J
J
Porter
J
J
Robinson
R
R
Pickup
C
C
Edwards
D000372:Agglutination Tests; D000907:Antibodies, Bacterial; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003208:Concanavalin A; D004755:Enterobacteriaceae / Q000302:isolation & purification* / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology / Q000302:isolation & purification; D018189:Immunomagnetic Separation / Q000379:methods*; D037102:Lectins; D012722:Sewage; D014871:Water Microbiology; D014876:Water Pollution
D000907:Antibodies, Bacterial; D037102:Lectins; D012722:Sewage; D003208:Concanavalin A