Localization of Dlg at the mammalian neuromuscular junction.
Recent studies have begun to elucidate the localization of ion channels and receptors in central nervous system synapses. A family of proteins containing PDZ domains has been suggested to play essential roles in these processes. PSD-95 and chapsyn-110 have been implicated in the clustering of Shaker K+ channels and NMDA receptors in the mammalian brain, and Dlg plays a role in the clustering of Shaker K+ channels at the Drosophila neuromuscular junction (NMJ). We have explored whether Dlg might participate in mammalian NMJ organization. We demonstrate that Dlg is expressed in muscle and co-localizes with utrophin at the post-synaptic face of the mammalian NMJ. Dlg may therefore be important for establishing or maintaining the organization of protein complexes at the mammalian NMJ.
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
J A
JA
Rafael
T L
TL
Hutchinson
C N
CN
Lumeng
S M
SM
Marfatia
A H
AH
Chishti
J S
JS
Chamberlain
D000818:Animals; D015153:Blotting, Western; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism; D004247:DNA / Q000302:isolation & purification; D019085:Fluorescent Antibody Technique, Direct; D005804:Genes, Lethal / Q000502:physiology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D009469:Neuromuscular Junction / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012313:RNA / Q000096:biosynthesis / Q000235:genetics; D049411:Utrophin
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; C483600:Utrn protein, mouse; D049411:Utrophin; D012313:RNA; D004247:DNA