Rapid growth mutants of Escherichia coli.
If rapid growth (rap) mutants of Escherichia coli could be obtained, these might prove a valuable contribution to fields as diverse as growth rate control, biotechnology and the regulation of the bacterial cell cycle. To obtain rap mutants, a dnaQ mutator strain was grown for four and a half days continuously in batch culture. At the end of the selection period, there was no significant change in growth rate. The result means that selecting rap mutants may require an alternative strategy and a number of such alternatives are discussed.
Department of Microbiology and Immunology, University of Leicester, UK.
J
J
Canvin
S
S
Grant
P
P
Freestone
I
I
Toth
M
M
Trinei
K
K
Modha
D
D
Cellier
V
V
Norris
D001431:Bacteriological Techniques; D002453:Cell Cycle / Q000235:genetics; D002455:Cell Division / Q000235:genetics*; D004261:DNA Replication / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D009154:Mutation / Q000235:genetics*; D012641:Selection, Genetic