Immunological studies on DNA-lysine photoadduct.
Calf thymus native DNA was covalently linked with lysine by irradiation with 254 nm light. Thermal melting studies indicated a decrease of 16 degrees C in the Tm of DNA-lysine photoadduct over native DNA. The decrease in Tm was dependent of irradiation dose. The photoadduct was found to be a potent immunogen in experimental animal. Thymine-lysine photoconjugate in DNA-lysine photo-monoadduct appears to be the major immunodominant portion. A strong recognition of DNA-lysine photoadduct was observed with anti-DNA autoantibodies found in the sera of patients with SLE. Thymine-lysine conjugate in DNA-lysine photo-monoadduct appears to provide an immunodominant epitope(s) for SLE autoantibody recognition. The results suggests for the possible involvement of DNA-lysine photoadduct or similar modified structure(s) as a potential trigger for anti-DNA autoantibody production.
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
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D000974:Antibodies, Antinuclear / Q000276:immunology; D000915:Antibody Affinity; D018736:DNA Adducts / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology; D009690:Nucleic Acid Conformation; D011234:Precipitins / Q000032:analysis
D000974:Antibodies, Antinuclear; D018736:DNA Adducts; C093096:DNA-lysine adduct; D011234:Precipitins; D008239:Lysine