Isolation of anti-nucleosome antibodies from the plasma of lupus nephritis patients.
Anti-nucleosome antibodies, which recognise conformational epitopes consisting of histone and DNA in chromatin, have been described in autoimmune diseases. In this study, an attempt was made to isolate anti-nucleosome antibodies from the anti-DNA-depleted plasma IgG of two lupus patients either with or without nephritis by nucleohistone affinity chromatography. The purified nucleohistone-binding antibodies bound to nucleohistone in a specific manner and contained enriched anti-histone antibodies. However, adsorption of the purified antibodies with histone revealed that the nephritis patient-derived antibodies contained nucleohistone-specific antibodies. Although such purified antibodies may not recognise native structures of nucleosomes, this chromatography may provide a method to isolate and determine the fine specificity of anti-nucleosome antibodies in various autoimmune diseases.
Immunology Research Laboratory, St Luke's Hospital, Kansas City, Missouri 64111, USA.
R
R
Suenaga
K
K
Mitamura
N I
NI
Abdou
D000974:Antibodies, Antinuclear / Q000097:blood*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D006657:Histones / Q000276:immunology; D006801:Humans; D008181:Lupus Nephritis / Q000276:immunology*; D009707:Nucleosomes / Q000276:immunology; D012680:Sensitivity and Specificity
D000974:Antibodies, Antinuclear; D006657:Histones; D009707:Nucleosomes