The single mutation Trp35-->Ala in the 35-40 redox site of Chlamydomonas reinhardtii thioredoxin h affects its biochemical activity and the pH dependence of C36-C39 1H-13C NMR.
The role of the invariant Trp residue at the redox site of thioredoxins was investigated by site-directed mutagenesis of a Chlamydomonas reinhardtii thioredoxin h. Though being still redox active with NADPH-thioredoxin reductase and chemical substrates [dithiothreitol and 5,5'-dithio-bis(2-nitrobenzoic acid)] the Trp35-->Ala-mutated protein completely lost the capacity to activate the thiol-regulated NADPH-dependent malate dehydrogenase. However, it was able to activate a mutant malate dehydrogenase where only the most exposed disulfide was retained. The pH dependence of the redox-site Cys beta 1H/13C-NMR frequencies of the wild-type and mutated proteins, in both the reduced and oxidised states, were compared over the pH range 5.8-10. The mutation does not affect the conserved buried Asp30, which titrates with a pKa of 7.5 in the oxidised proteins in agreement with previous studies. However, for the reduced forms of the proteins, the pH dependence of resonances of both Cys was strongly affected by the mutation. In the case of the wild-type thioredoxin, two apparent pKa values were found around 7.0 and 9.5 and could be assigned to the titration of Cys36 and Cys39 thiol, respectively, similar to the case of Escherichia coli thioredoxin. For the mutated thioredoxin a single pKa was found around 8.3. This result can be interpreted as a single pKa of either Cys36 or Cys39 or both. While the mutation clearly affects ionisations, the measured redox potentials of the active-site Cys pair are not significantly affected by the Trp35-->Ala mutation. Possible roles of an aromatic side chain on the reactivity of the catalytic Cys residues in thioredoxins are proposed.
Laboratoire de RMN Biomoléculaire associé au CNRS, Université Claude Bernard-Lyon 1, Villeurbanne, France.
I
I
Krimm
S
S
Lemaire
E
E
Ruelland
M
M
Miginiac-Maslow
J P
JP
Jaquot
M
M
Hirasawa
D B
DB
Knaff
J M
JM
Lancelin
D000818:Animals; D001665:Binding Sites; D002247:Carbon Isotopes; D016825:Chlamydomonas reinhardtii; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D008291:Malate Dehydrogenase / Q000378:metabolism; D050538:Malate Dehydrogenase (NADP+); D008958:Models, Molecular; D009154:Mutation; D019906:Nuclear Magnetic Resonance, Biomolecular; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D011199:Potentiometry; D011994:Recombinant Proteins / Q000378:metabolism; D054480:Thioredoxin h; D013879:Thioredoxins / Q000235:genetics / Q000378:metabolism*; D014364:Tryptophan
D002247:Carbon Isotopes; D010940:Plant Proteins; D011994:Recombinant Proteins; D054480:Thioredoxin h; D013879:Thioredoxins; D006859:Hydrogen; D014364:Tryptophan; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+)