Vaccinia virus protein B18R inhibits the activity and cellular binding of the novel type interferon-delta.
The soluble vaccinia virus-encoded protein B18R inhibits the antiviral activity and cellular binding of the type I interferons (IFN)-alpha, -beta and -omega of different mammalian species. Recently, a novel type I IFN was detected in pigs and classified as a member of a distinct IFN family designated IFN-delta. Our study aimed to determine if the structural properties of this shortest (149 residues long) type I IFN allow its interaction with the type I IFN-binding protein B18R. Experiments using bovine (MDBK) cells demonstrated that B18R neutralized the antiviral activity of porcine IFN-delta with high efficiency. Preincubation of B18R with radiolabelled IFN-delta specifically inhibited binding of IFN to bovine cells. These data indicate that the overall conformation of the novel IFN-delta might be similar to that of other type I IFNs.
Institute of Virology, Slovak Academy of Sciences, Bratislava.
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Vancová
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La Bonnardiere
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Kontsek
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism*; D002417:Cattle; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism*; D017471:Receptors, Interferon / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013552:Swine; D014616:Vaccinia virus / Q000378:metabolism* / Q000502:physiology; D014764:Viral Proteins / Q000378:metabolism*
D000998:Antiviral Agents; D007370:Interferon Type I; D017471:Receptors, Interferon; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C037655:interferon, delta; C067853:B18R protein, Vaccinia virus