Water immersion-restraint stress induces expression of immediate-early genes in gastrointestinal tract of rats.
The aims of this study were to determine 1) which cells are involved in stress-induced acute gastric mucosal lesion and 2) what kinds of molecular alterations are induced by stress, using immediate-early genes (IEG) as tools for detection of cellular activation. Male Wistar rats were exposed to acute water immersion-restraint stress. Protein and mRNA for IEG were detected by immunohistochemistry and in situ hybridization, respectively. This stress induced the expression of c-fos and nerve growth factor-induced gene (NGFI-A) mRNA in gastric epithelial cells, the smooth muscle layer of small blood vessels, and the stomach wall. Stress upregulated the mRNA levels of these IEG in the duodenal epithelial cells and induced de novo expression of IEG in the smooth muscle layer of small blood vessels and the duodenal wall. These findings indicate that these cells are activated in response to stress. Expression of these IEG and/or transcriptional factors may reflect an initiation of mechanisms for repairing the lesions induced by stress as well as an adaptation to the stress.
Department of Anatomy and Neurobiology, Wakayama Medical College, Wakayama, 641-0012, Japan.
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Ueyama
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Saika
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Koreeda
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Senba
D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D004386:Duodenum / Q000098:blood supply / Q000378:metabolism*; D051766:Early Growth Response Protein 1; D005753:Gastric Mucosa / Q000378:metabolism*; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D016762:Genes, fos; D016758:Genes, jun; D017874:Immediate-Early Proteins; D007101:Immersion; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D009130:Muscle, Smooth / Q000098:blood supply / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D014176:Protein Biosynthesis; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis / Q000235:genetics; D016755:Proto-Oncogene Proteins c-jun / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D012149:Restraint, Physical; D013270:Stomach / Q000098:blood supply; D013315:Stress, Psychological / Q000378:metabolism*; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics; D014158:Transcription, Genetic; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D051766:Early Growth Response Protein 1; C486110:Egr1 protein, rat; D017874:Immediate-Early Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D014157:Transcription Factors