Kinetics of 131I-beta2 microglobulin in hemodialysis patients: assessment using total body counting.
The kinetics of 131I-beta2-microglobulin (beta2-M) were studied using external total body gamma counting in a low noise chamber after administration of trace doses of radioactivity (4 microCi) in 14 uremic patients treated by either hemodialysis or hemofiltration. Data were collected over a 1 week period that included 3 dialysis sessions. The following artificial membranes were used: Cuprophan, polyacrylonitrile AN69, polysulfone, polymethylmethacrylate (PMMA), and polyamide. Radiolabeled beta2-M excretion by an extrarenal route was nearly nonexistent. The 131I-beta2-M half-life was between 2.4 and 8 days, shorter in patients with residual diuresis. A mean removal of 153+/-33 mg/L of beta2-M was obtained per dialysis session with a highly permeable membrane. A hemofiltration session (25 L exchange per session) was slightly more efficient in removing beta2-M than a 4 h hemodialysis session with the same AN69 highly permeable membrane. The amounts of 131I-beta2-M binding on the membranes, expressed as beta2-M equivalents, were 0, 16, 54, 58, and 59 mg/m2 for Cuprophan, polysulfone, polyacrylonitrile AN69, polyamide, and PMMA, respectively. In conclusion, the decrease of total body gamma counting directly reflected the beta2-M breakdown and removal in hemodialysis patients. Intact beta2-M was removed by convection with synthetic, highly permeable membranes. In addition, membrane adsorption accounted for 15% (polysulfone) to near 100% (PMMA) of the beta2-M removal per session. Adsorption was of the same magnitude regardless of the dialysis technique in use, indicating a membrane saturability process. None of the currently available dialysis procedures based on a 3 sessions per week schedule can balance beta2-M generation.
Chanard
J
J
Service de Néphrologie, Centre Hospitalier et Universitaire, Reims, France.
Caudwell
V
V
Valeire
J
J
Vincent
C
C
Randoux
C
C
Wuillai
A
A
Wynckel
A
A
D000180:Acrylic Resins / Q000737:chemistry; D000181:Acrylonitrile / Q000031:analogs & derivatives / Q000737:chemistry; D000327:Adsorption; D000328:Adult; D000368:Aged; D001672:Biocompatible Materials / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry; D005260:Female; D006207:Half-Life; D006440:Hemofiltration / Q000295:instrumentation; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D009757:Nylons / Q000737:chemistry; D010539:Permeability; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry; D019275:Radiopharmaceuticals; D006435:Renal Dialysis / Q000295:instrumentation; D013450:Sulfones / Q000737:chemistry; D014511:Uremia / Q000628:therapy; D014915:Whole-Body Counting; D001613:beta 2-Microglobulin / Q000737:chemistry / Q000493:pharmacokinetics*
D000180:Acrylic Resins; D001672:Biocompatible Materials; D007457:Iodine Radioisotopes; D008567:Membranes, Artificial; D009757:Nylons; D011108:Polymers; D019275:Radiopharmaceuticals; D013450:Sulfones; D001613:beta 2-Microglobulin; C010504:polyacrylonitrile; C017662:polysulfone P 1700; C016892:AN-69; D002482:Cellulose; D019904:Polymethyl Methacrylate; C009865:cuprammonium cellulose; D000181:Acrylonitrile