Increase in brain stem cytokine mRNA levels as an early response to chemical-induced myelin edema.
This study examined the early response of pro-inflammatory and regulatory cytokines in the mouse brain following triethyltin (TET)-induced myelin injury characterized by edematous vacuolation. Following an acute intraperitoneal injection of triethyltin (TET) sulfate (3 mg/kg) to 17-day old CD1 mice, significant increases in brain stem TNF-alpha and IL-1alpha mRNA levels occurred at 6 and 24 h, respectively with elevations in TGF-beta1 and MIP-1alpha at 1 h. In the cortex, responses were limited to elevations at 6 h in TNF-alpha, TGF-beta1 and MIP-1alpha. These data suggest that a chemokine/cytokine response can occur with minimal alterations to the integrity of the myelin sheath and may contribute to the initial signaling mechanisms associated with demyelinating disorders.
Mehta
P S
PS
Department of Chemistry, University of North Carolina at Chapel Hill, 27599, USA.
Bruccoleri
A
A
Brown
H W
HW
Harry
G J
GJ
D000818:Animals; D001929:Brain Edema / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000235:genetics*; D017403:In Situ Hybridization; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012260:Ribonucleases; D016212:Transforming Growth Factor beta / Q000235:genetics; D014267:Triethyltin Compounds / Q000494:pharmacology
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014267:Triethyltin Compounds; C031209:Triethyltin sulfate; D012260:Ribonucleases