Patterns of messenger RNA expression for alpha1-adrenoceptor subtypes in human corpus cavernosum.
OBJECTIVE
The present study evaluated the expression of alpha1-adrenoceptor subtypes in human corpus cavernosum.


METHODS
The mRNA encoding alpha1a, alpha1b and alpha1d subtypes were assessed by RNA-directed complementary cDNA synthesis followed by Taq DNA amplification. The level of alpha1 mRNA was calculated in arbitrary optical density units and normalized with respect to the length of the respective cDNA fragments.


RESULTS
We found that alpha1a, alpha1b and alpha1d adrenoceptor subtypes are expressed in human corpus cavernosum, with a predominant expression of the alpha1a subtype.


CONCLUSIONS
These results suggest that alpha1a-adrenoceptor subtype is important and that understanding the biochemical and functional characteristics of this subtype may lead to the development of specific antagonists in the treatment of impotence.
CJF-INSERM 94.02, Signalisation et Transduction, Faculté de Médecine de Paris-Ouest, France.
J P
JP
Dausse
A
A
Leriche
F
F
Yablonsky
D000199:Actins / Q000235:genetics; D058646:Adrenergic alpha-1 Receptor Agonists; D058668:Adrenergic alpha-1 Receptor Antagonists; D000328:Adult; D000368:Aged; D018076:DNA, Complementary / Q000235:genetics; D007172:Erectile Dysfunction / Q000188:drug therapy; D005784:Gene Amplification; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010413:Penis / Q000378:metabolism*; D011317:Priapism / Q000188:drug therapy; D011467:Prostate / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics*; D018340:Receptors, Adrenergic, alpha-1 / Q000145:classification / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics
D000199:Actins; D058646:Adrenergic alpha-1 Receptor Agonists; D058668:Adrenergic alpha-1 Receptor Antagonists; D018076:DNA, Complementary; D012333:RNA, Messenger; D018340:Receptors, Adrenergic, alpha-1