Cross-reactivity of an anti-okadaic acid antibody to dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid diol ester.
The cross-reactivity of the 6/50 monoclonal anti-okadaic acid antibody (mAb) to the recently discovered diarrhetic shellfish poisoning (DSP) metabolites dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid (OA) diol ester was determined using a competitive indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of the antibody to these molecules was compared to that with OA; the 6/50 mAb recognized all of these DSP compounds with equal sensitivity within the working range of the antibody (10-100 nM for OA). This confirms the ability of the antibody to detect all DSP compounds when used in analyses including ELISA and immunocytochemistry.
Lawrence
J E
JE
Department of Oceanography, Dalhousie University, Halifax, NS, Canada.
Cembella
A D
AD
Ross
N W
NW
Wright
J L
JL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D004141:Dinoflagellida / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D008387:Marine Toxins / Q000737:chemistry / Q000276:immunology*; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; C114941:dinophysistoxin 5; C110249:okadaic acid diol ester; C114940:dinophysistoxin 4; D019319:Okadaic Acid