Binding of the Helicobacter pylori vacuolating cytotoxin to target cells.
The vacuolating cytotoxin of Helicobacter pylori, VacA, enters the cytoplasm of target cells and causes vacuolar degeneration by interfering with late stages of endocytosis. By using indirect immunofluorescence and flow cytometry, we have demonstrated that VacA binds to specific high-affinity cell surface receptors and that this interaction is necessary for cell intoxication.
Massari
P
P
IRIS, Chiron Vaccines, 53100 Siena, Italy.
Manetti
R
R
Burroni
D
D
Nuti
S
S
Norais
N
N
Rappuoli
R
R
Telford
J L
JL
D016475:3T3 Cells; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001427:Bacterial Toxins / Q000378:metabolism*; D001667:Binding, Competitive; D002460:Cell Line; D003603:Cytotoxins / Q000378:metabolism*; D006367:HeLa Cells; D016480:Helicobacter pylori / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism*
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D003603:Cytotoxins; D011956:Receptors, Cell Surface; C074689:VacA protein, Helicobacter pylori