A putative HCO3- transporter in the cyanobacterium Synechococcus sp. strain PCC 7942.
Cyanobacteria possess an inducible mechanism which enables them to concentrate inorganic carbon (Ci) within the cells. An inactivation library was used to raise the high-CO2-requiring mutant of Synechococcus PCC 7942, IL-2, impaired in HCO3- transport. Analysis of the relevant genomic DNA detected several modifications, probably due to the single crossover recombination, leading to inactivation of ORF467 (designated ictB) in IL-2. IctB contains 10 trans-membrane regions and is homologous to several transport-related proteins from various organisms. Kinetic analyses of HCO3- uptake in the wild type and IL-2 suggested the presence of two or three HCO3- carriers exhibiting different affinities to HCO3-.
Department of Plant Sciences, the Moshe Shilo and the Avron-Evenari Minerva Centers, The Hebrew University of Jerusalem, Israel.
D J
DJ
Bonfil
M
M
Ronen-Tarazi
D
D
Sültemeyer
J
J
Lieman-Hurwitz
D
D
Schatz
A
A
Kaplan
D001639:Bicarbonates / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002254:Carbonates / Q000378:metabolism; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D004252:DNA Mutational Analysis; D015723:Gene Library; D007376:Interleukin-2 / Q000235:genetics / Q000502:physiology*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid
D001639:Bicarbonates; D002254:Carbonates; D007376:Interleukin-2; D002245:Carbon Dioxide