Enhanced liver cell mutations in trematode-infected Big Blue transgenic mice.
Parasite infections in humans have long been associated with specific types of cancers. Schistosoma hematobium is a known inducer of urinary bladder cancer, Helicobacter pylori is a gastric carcinogen, and hepatitis B virus and Opisthorchis viverrini are causative agents of liver cell cancers. Another liver fluke, Fasciola hepatica, has also been identified as a neoplastic risk agent, primarily in animals. We used F. hepatica as a model agent to determine if the presence of an aggressive liver fluke could induce mutagenic events in mammalian tissue. Using the Big Blue(R) transgenic mouse assay, we found a two-fold increase in lacI mutations in cells harvested from mice harboring F. hepatica worms when compared to uninfected control animals. These data indicate that biological infections can cause increased genetic damage in surrounding host tissue.
Biology Department, Hope College, Holland, MI 49423, USA. gentile@hope.edu
J M
JM
Gentile
G J
GJ
Gentile
B
B
Nannenga
M
M
Johnson
H
H
Blankespoor
R
R
Montero
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D003090:Coliphages / Q000235:genetics; D004252:DNA Mutational Analysis; D018509:DNA, Helminth / Q000032:analysis; D029968:Escherichia coli Proteins; D005210:Fasciola hepatica / Q000472:pathogenicity*; D005211:Fascioliasis / Q000235:genetics* / Q000473:pathology; D056968:Lac Repressors; D008099:Liver / Q000737:chemistry* / Q000378:metabolism* / Q000469:parasitology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009154:Mutation; D012097:Repressor Proteins / Q000235:genetics; D019076:Transgenes / Q000235:genetics
D001426:Bacterial Proteins; D018509:DNA, Helminth; D029968:Escherichia coli Proteins; D056968:Lac Repressors; D012097:Repressor Proteins