Further development of a recombinant feline herpesvirus type 1 vector expressing feline calicivirus immunogenic antigen.
We previously reported the attenuation of thymidine kinase (TK) deficient mutant (C7301dlTK) of feline herpesvirus type 1 (FHV-1) in cats and the construction of a recombinant FHV-1 (C7301dlTK-Cap) inserted a precursor capsid gene of feline calcivirus (FCV) into the TK deletion locus of the C7301dlTK. In this study, we constructed a further improved recombinant FHV-1 (dlTK(gCp)-Cap) carrying a putative FHV-1 gC promoter sequence upstream of the FCV precursor capsid gene of the C7301dlTK-Cap. Growth kinetics of the dlTK(gCp)-Cap in cell cultures was similar to those of C7301dlTK and C7301dlTK-Cap. A strong expression of FCV immunogenic antigen by dlTK(gCp)-Cap was confirmed by indirect immunofluorescence and enzyme-linked immunosorbent assays. In addition, one vaccination with dlTK(gCp)-Cap protected cats more effective against subsequent virulent FCV challenge than that with C7301dlTK-Cap.
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
N
N
Yokoyama
K
K
Fujita
A
A
Damiani
E
E
Sato
K
K
Kurosawa
T
T
Miyazawa
S
S
Ishiguro
M
M
Mochizuki
K
K
Maeda
T
T
Mikami
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation; D017927:Calicivirus, Feline / Q000235:genetics / Q000276:immunology*; D002213:Capsid / Q000235:genetics; D002371:Cat Diseases / Q000276:immunology* / Q000517:prevention & control; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology*; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D013937:Thymidine Kinase / Q000235:genetics; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C090200:glycoprotein C, Felid herpesvirus 1; D013937:Thymidine Kinase