Fibrin glue as a delivery vehicle for autologous urothelial cell transplantation onto a prefabricated pouch.
OBJECTIVE
The purpose of the study was to investigate how in vivo expanded urothelium can be transplanted onto a prefabricated capsule-pouch for urinary reconstruction.


METHODS
Urothelial cells from 40 rats were harvested for culture. A tissue expander was used to induce a capsule-pouch on which the cell culture were reimplanted ten days later. As delivery vehicle we compared standard culture media and fibrin glue.


RESULTS
The histology demonstrated viable, multilayered clusters of urothelium cells only in the group using the fibrin glue delivery vehicle.


CONCLUSIONS
We conclude that cultured urothelial cells can be successfully reimplanted onto a prefabricated capsule-pouch via fibrin glue, showing potential for urinary reconstructions.
Department of Plastic and Reconstructive Surgery, Leopold-Franzens University, Innsbruck, Austria.
G
G
Wechselberger
T
T
Schoeller
A
A
Stenzl
M
M
Ninkovic
S
S
Lille
R C
RC
Russell
D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D017690:Cell Transplantation; D002478:Cells, Cultured; D004396:Coloring Agents; D003239:Connective Tissue Cells / Q000166:cytology; D003470:Culture Media; D016207:Cytokines / Q000032:analysis; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000166:cytology; D015718:Fibrin Tissue Adhesive / Q000627:therapeutic use*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019651:Plastic Surgery Procedures; D019738:Surgically-Created Structures; D014014:Tissue Adhesives / Q000627:therapeutic use*; D015626:Tissue Expansion; D015625:Tissue Expansion Devices; D001743:Urinary Bladder / Q000166:cytology / Q000601:surgery; D019459:Urothelium / Q000166:cytology*
D004396:Coloring Agents; D003470:Culture Media; D016207:Cytokines; D015718:Fibrin Tissue Adhesive; D014014:Tissue Adhesives