Y;16 translocation breakpoint associated with a partial Turner phenotype identifies a foamy virus insertion.
We have previously identified YACs containing the Y breakpoint in a male patient with Turner-like hydrops in the newborn period and a Y;16 translocation (Erickson et al., 1995). We have now subcloned these YACs and built a lambda contig across the Y breakpoint. A subclone near the Y;16 breakpoint of our patient shows great evolutionary conservation by hybridization to a zoo blot. We have sequenced the region responsible for the hybridization and found a foamy (spuma) virus sequence. These viruses are known to be xenotropic and a very similar cross-species hybridization (using the same commercial zoo blot) was recently reported with a human foamy virus clone (Cordonnier et al., 1995). Thus, we have identified a foamy virus insertion near the Y heterochromatin boundary.
Department of Human Genetics and Pediatrics, University of Michigan School of Medicine, Ann Arbor, USA.
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Schmidt Drury
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Erickson
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Glover
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002645:Chickens; D004279:DNA, Viral; D004285:Dogs; D000882:Haplorhini; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010641:Phenotype; D011817:Rabbits; D051381:Rats; D016092:Spumavirus / Q000235:genetics*; D014178:Translocation, Genetic; D014424:Turner Syndrome / Q000235:genetics*; D014998:Y Chromosome
D004279:DNA, Viral