Cloning, characterization, and transcriptional analysis of a gene encoding an alpha-crystallin-related, small heat shock protein from the thermophilic cyanobacterium Synechococcus vulcanus.
hspA, a gene encoding a 16-kDa heat-induced protein from the thermophilic cyanobacterium Synechococcus vulcanus, has been cloned and sequenced. The deduced amino acid sequence of the gene product showed significant homology to sequences of the family of alpha-crystallin-related, small heat shock proteins. A monocistronic mRNA of hspA increased transiently in response to heat shock. The heat shock induction occurred at a vegetative promoter but without the CIRCE (controlling inverted repeat of chaperone expression) element.
Department of Biochemistry and Molecular Biology, Saitama University, Urawa 338, Japan.
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Roy
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Nakamoto
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D003459:Crystallins / Q000737:chemistry*; D000458:Cyanobacteria / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D005798:Genes, Bacterial; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D006358:Hot Temperature; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis / Q000737:chemistry / Q000187:drug effects; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D012293:Rifampin / Q000494:pharmacology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003459:Crystallins; D017931:DNA Primers; D006360:Heat-Shock Proteins; C113725:HspA protein, bacteria; D012333:RNA, Messenger; D011994:Recombinant Proteins; D012293:Rifampin