Ribosome-inactivating proteins (RIPs) of wild Oregon cucumber (Marah oreganus).
Two type 1 RIPs, designated as MOR-I and MOR-II, have been isolated from Marah oreganus (manroot) seed extract. They are similar but not identical to trichosanthin, a type 1 RIP in the same family. MOR-I and MOR-II are monomeric proteins with molecular weights of 27989.0 and 27632.8 respectively and have pI values greater than 8.8. MOR-I and MOR-II inhibit cell-free protein synthesis with IC50s of 0.063 and 0.071 nM, respectively, and are relatively stable with respect to temperature and pH variations. They share a conserved N-terminal amino acid sequence (D-SF-LS) and cross-react with goat anti-trichosanthin polyclonal serum.
Department of Chemical Engineering and Materials Science, University of California Davis, 95616, USA.
N J
NJ
Shih
K A
KA
McDonald
T
T
Girbés
R
R
Iglesias
A J
AJ
Kohlhoff
A P
AP
Jackman
D000818:Animals; D015153:Blotting, Western; D006026:Glycoside Hydrolases / Q000378:metabolism*; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism*
D010940:Plant Proteins; D006026:Glycoside Hydrolases