An alternative for purification of low soluble recombinant hepatitis C virus core protein: preparative two-dimensional electrophoresis.
For isolation of low soluble recombinant full-length (amino acids 1-191) core protein of hepatitis C virus (HCV) overexpressed in Escherichia coli, the advantage of combining two electrophoretic techniques, in comparison with chromatographic separation, is demonstrated. The protein extract was first solubilized in agents compatible with electrophoretic separation. Using preparative liquid phase isoelectric focusing (IEF) the protein of interest was first concentrated within a defined acidic pH range. These fractions were then submitted to preparative sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) to isolate the 22 kDa protein. The second-dimensional step allowed the isolation of 2 mg of the purified recombinant HCV core protein (rHCV-C191) from 1.5 g bacterial pellet. This quantity is sufficient to characterize the protein and to perform immunogenicity studies. This procedure of two-dimensional preparative electrophoresis is applicable to a wide range of biological samples and represents an alternative for purification of insoluble proteins.
Département Recherche et Développement, Unité Immunoassays, BioMérieux S.A., Marcy-l'Etoile, France.
S
S
Yvon
D
D
Rolland
J P
JP
Charrier
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M
Jolivet
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine; D006801:Humans; D007525:Isoelectric Focusing; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification; D012995:Solubility; D014758:Viral Core Proteins / Q000235:genetics / Q000302:isolation & purification*
D011993:Recombinant Fusion Proteins; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D006639:Histidine