Tissue distribution and cellular localization of the levocabastine-sensitive neurotensin receptor mRNA in adult rat brain.
The aim of this study was to determine the regional and cellular distribution of the neurotensin type 2 receptor (NT-2R) mRNA in the rat brain. Using a radioactive in situ hybridization approach, the distribution of NT-2R transcripts was quantified from autoradiograms, and the cellular localization was examined in liquid emulsions. In rat brain, NT-2R mRNAs, are more widespread than the neurotensin type 1 receptor mRNA. NT-2R transcripts are diffusely distributed throughout the brain, with higher quantities found in the pia mater, the ventricles, the subfornical organ, the subiculum, the substantia nigra, the ventral tegmental area, the superior colliculus, the periaqueductal gray matter, the Purkinje cells and certain hypothalamic and brainstem nuclei. At the cellular level, silver grains appear to be concentrated on glia, neurons and ependymal cells, such as cell bodies of the glia-rich corpus callosum, Purkinje neurons in the cerebellum and ependymal cells lining the ventricles. In contrast, the thalamus and the amygdala contain low amounts of NT-2R mRNA. We discuss the anatomical location of NT-2R mRNA in relation to possible roles for this new receptor subtype.
Walker
N
N
Sanofi Recherche, Labege, France. nancy.walker@tls1.elfsanofi.fr
Lepee-Lorgeoux
I
I
Fournier
J
J
Betancur
C
C
Rostene
W
W
Ferrara
P
P
Caput
D
D
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D001933:Brain Stem / Q000187:drug effects; D002531:Cerebellum / Q000187:drug effects; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D017403:In Situ Hybridization; D008297:Male; D015345:Oligonucleotide Probes; D010880:Piperidines / Q000494:pharmacology*; D016548:Prosencephalon / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018028:Receptors, Neurotensin / Q000235:genetics*; D014018:Tissue Distribution / Q000502:physiology
D006634:Histamine H1 Antagonists; D015345:Oligonucleotide Probes; D010880:Piperidines; D012333:RNA, Messenger; D018028:Receptors, Neurotensin; C047340:levocabastine