[Incidence and elimination of false-negative results of ferritin determination].
Introduction of turbidimetric homogeneous immunoassays made the determination of plasma ferritin concentration wide-ranging available. However, high-dose hook effect or prozone effect occurring at samples with high ferritin concentration can lead to false-negative results. According to the authors, this phenomenon has considerable clinical significance, in patients with iron-overload disorders false-negative laboratory values may result in inaccurate diagnosis. The prozone effect can be eliminated by reaction kinetic analysis of measurements. The authors developed a neural network classification procedure based on artificial intelligence technology for the recognition of the reactions with differing kinetic flow, and made a computer software for helping the application of the classification system. False-negative results can be filtered using this new technology following the laboratory determination, thus sensitivity of plasma ferritin determination may become safe enough even in case of high concentration samples.
II. Belgyógyászati Klinika, Semmelweis Orvostudományi Egyetem, Budapest.
K
K
Papik
B
B
Molnár
P
P
Fedorcsák
S
S
Rainer
L
L
Fridl
J
J
Fehér
Z
Z
Tulassay
D005188:False Negative Reactions; D005293:Ferritins / Q000097:blood*; D006402:Hematologic Diseases / Q000097:blood; D006801:Humans; D007118:Immunoassay; D008107:Liver Diseases / Q000097:blood; D009391:Nephelometry and Turbidimetry; D006435:Renal Dialysis
D005293:Ferritins