Characterization of protease(s) in adults of Trichuris globulosa (Nematoda: Trichuridae).
The activity of protease(s) has been observed separately in male and female Trichuris globulosa. Different fractions showed optimum protease activity at 37 degrees C, pH 9.5 and 1.5 mg casein concentration. Excretory/secretory (E/S) products showed maximum protease activity, which might be helpful in the degradation of mucosal tissue of the host gut. Male parasites appear to feed extensively on connective tissue collagen/elastin, while females feed on both connective tissue and body/cellular fluids. Inhibition/activation studies revealed the presence of four kinds of protease in the E/S products, cytosolic- and membrane-bound fractions performing different functions.
Department of Zoology, Punjab Agricultural University, Ludhiana, India.
D K
DK
Kocher
M L
ML
Sood
D000821:Animal Feed; D000818:Animals; D003106:Colon / Q000469:parasitology; D003600:Cytosol / Q000201:enzymology; D010450:Endopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006041:Goats / Q000469:parasitology; D007700:Kinetics; D008297:Male; D012727:Sex Characteristics; D014258:Trichuris / Q000201:enzymology* / Q000302:isolation & purification
D010450:Endopeptidases