Dissolved oxygen determination by electrocatalysed chemiluminescence with in-line solid phase media.
Dissolved elemental oxygen is determined in a flowing aqueous stream using glucose oxidase to catalyse the reaction between D-glucose and O2 to produce hydrogen peroxide. The levels of the resulting H2O2 are detected and quantified by luminol chemiluminescence using in-line solid phase media for pH adjustment of the reagent stream and for controlled release of the luminophore. The reaction is initiated by electrochemical catalysis. By the use of excess D-glucose in the reagent flow stream, the intensity of chemiluminescence is rendered proportional only to fluctuations in the dissolved O2 concentration. The methodology provides a means for the detection of aqueous O2 in the range 0-10 mg/L.
Atwater
J E
JE
UMPQUA Research Company, Myrtle Creek, OR 97457, USA.
DeHart
J
J
Wheeler
R R
RR
D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004800:Enzymes, Immobilized; D005947:Glucose; D005949:Glucose Oxidase; D006861:Hydrogen Peroxide / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008163:Luminescent Measurements; D008165:Luminol; D010100:Oxygen / Q000032:analysis*; D012680:Sensitivity and Specificity; D012996:Solutions
D004800:Enzymes, Immobilized; D007202:Indicators and Reagents; D012996:Solutions; D008165:Luminol; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D005947:Glucose; D010100:Oxygen