Mapping of porcine genes belonging to two different cytochrome P450 subfamilies.
Three cDNA clones from a porcine small intestine cDNA library, tentatively identified as transcripts of two different cytochrome P450 (CYP) gene subfamilies, were sequenced and mapped. One of the clones was found to comprise an open reading frame of 503 amino acids and showed up to an 80% similarity to genes from the CYP3A subfamily. Translation of the other two cDNA clones revealed high similarity to genes from the CYP2C subfamily. One of these clones was truncated, lacking a part of the 5' end of the coding region, whereas the other encodes a putative pseudogene. The CYP3A gene was mapped cytogenetically by means of a pig/rodent somatic hybrid cell panel to pig chromosome 3, in the region p16-p17 or p11. The CYP2C pseudogene was mapped genetically to pig chromosome 14, using the PiGMaP shared reference pedigrees. The localisations provide information for the human/pig comparative map.
Department of Animal Science and Animal Health, Royal Veterinary and Agricultural University, Copenhagen, Denmark.
P H
PH
Nissen
A K
AK
Winterø
M
M
Fredholm
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary*; D006224:Cricetinae / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006822:Hybrid Cells / Q000737:chemistry; D051379:Mice; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000662:veterinary; D018807:Polymorphism, Single-Stranded Conformational; D011544:Pseudogenes; D017422:Sequence Analysis, DNA / Q000662:veterinary*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D003577:Cytochrome P-450 Enzyme System