[Features of oxidative metabolism and level of platelet activating factor in pulmonary and circulating phagocytes of tuberculosis-resistant animals at stages of experimental infection].
An experiment was conducted on noninbred albino rats intraperitoneally inoculated with Mycobacteria tuberculosis (MBT) in a dose of 0.025 mg. Alveolar macrophages (AM), non fractionated cell sediments (NFCS) and circulating leukocytes were studied 1 day, 1, 2, and 6 weeks after inoculation. Spontaneous and killed BCG culture-stimulated killed HCT-test, the activity of superoxide dismutase (SOD) and catalase, the level of malonic dialdehyde (MDA) and platelet activation factor (PAF) were determined. Morphological changes were histologically controlled. The rats administered the MBT dose sufficient to initiate generalized tuberculosis in guinea pigs were found to develop nonspecific changes which became reversible by the end of the experiment. Throughout the process, there was no splash of basal oxidative metabolism in all phagocytic types and their functional decompensation did not develop. Following 1.5 months, all the types of phagocytes showed an increased oxygen burst during specific stimulation. The level of MDA did not exceed the control values in all periods. The activity of anti-oxidative enzymes changed heterodirectionally. At the final stage, the activity of catalase repeatedly increased with normal or reduced SOD values. In early infection, the concentration of PAF rose in the pulmonary phagocytes. When resistance was formed, in all cell types it fell below the control values.
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Kaminskaia
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RIu
Abdullaev
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Gedymin
D000818:Animals; D015415:Biomarkers; D002374:Catalase / Q000378:metabolism; D004195:Disease Models, Animal; D005500:Follow-Up Studies; D006168:Guinea Pigs; D007962:Leukocytes / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D010972:Platelet Activating Factor / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism; D014397:Tuberculosis, Pulmonary / Q000378:metabolism* / Q000473:pathology
D015415:Biomarkers; D010972:Platelet Activating Factor; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase