Differential cell cycle regulation by low- and high-risk human papillomaviruses in low-grade squamous intraepithelial lesions of the cervix.
In this study, we have demonstrated the overexpression of cyclin D1 protein in 24 (92%) of 26 low-grade squamous intraepithelial lesions infected with low-risk human papillomaviruses (HPV 6, 11, 42, 43, and 44) and the absence of cyclin D1 expression in 25 (87%) of 29 lesions infected with high-risk HPVs (HPV 16, 18, 31, 33, 39, 45, 51, 52, 56, 58, and 66). The expression of cyclins E, A, and B proteins was increased in both low-risk and high-risk HPV infections. Tetrasomy of chromosomes 1, 3, 11, 17, 18, and X was present in nine lesions, all of which were infected with high-risk HPVs, but was not related to the pattern of cyclin expression. These data provide in vivo evidence that low- and high-risk HPV types alter cell cycle control by different mechanisms and that cell cycle checkpoint abnormalities are induced by high-risk, but not low-risk, HPV infection.
Southern
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Department of Pathology, University of Liverpool, Royal Liverpool University Hospital, United Kingdom.
Herrington
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D002453:Cell Cycle; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D019938:Cyclin D1 / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism / Q000821:virology; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000145:classification / Q000502:physiology*; D030361:Papillomavirus Infections / Q000378:metabolism* / Q000473:pathology; D014412:Tumor Virus Infections / Q000378:metabolism* / Q000473:pathology; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D014860:Warts / Q000378:metabolism / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000378:metabolism* / Q000473:pathology / Q000821:virology
D019938:Cyclin D1