IL-12 promotes the adhesion of NK cells to endothelial selectins under flow conditions.
This study examined the adhesive interactions of peripheral blood NK cells with P- and E-selectin and analyzed the effect of IL-12 on the binding of NK cells to these selectins. P-selectin glycoprotein ligand-1 (PSGL-1) is expressed on most resting and IL-12-activated NK cells. However, the percentage of resting NK cells bound to P-selectin-IgG was 15%, and that of activated NK cells bound to P-selectin-IgG was 65%. Furthermore, the number of IL-12-activated NK cells bound to P-selectin-transfected Chinese hamster ovary cells was significantly higher than that of resting NK cells under flow conditions. These interactions were abolished by the incubation of these NK cells with anti-PSGL-1 (PL-1) mAb. Thus, PSGL-1/P-selectin interaction is important in the binding of resting and activated NK cells to P-selectin. NK cells express sialyl-Lewis(x) (sLe(x)) structure recognized by anti-sLe(x) mAb (KM-93), and IL-12 activation of NK cells increased the mean fluorescence intensity of KM-93-reactive NK cells. Adhesion of IL-12-activated NK cells to E-selectin-transfected Chinese hamster ovary cells was stronger than that of resting NK cells under flow conditions. These interactions were reduced markedly by incubation with anti-sLe(x) mAb. Thus, sLe(x) is the major ligand of resting and activated NK cells for E-selectin. These findings indicate that IL-12 stimulation of NK cells promotes their adhesion activity to endothelial selectins.
Department of Otorhinolaryngology, Yokohama City University, School of Medicine, Japan.
T
T
Yago
M
M
Tsukuda
H
H
Fukushima
H
H
Yamaoka
K
K
Kurata-Miura
T
T
Nishi
M
M
Minami
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D016466:CHO Cells; D002448:Cell Adhesion / Q000276:immunology; D006224:Cricetinae; D019040:E-Selectin / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D018664:Interleukin-12 / Q000502:physiology*; D007376:Interleukin-2 / Q000494:pharmacology; D007399:Interphase / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology* / Q000378:metabolism; D019041:L-Selectin / Q000276:immunology; D007983:Lewis Blood Group Antigens / Q000276:immunology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000276:immunology; D009844:Oligosaccharides / Q000096:biosynthesis / Q000276:immunology; D019007:P-Selectin / Q000276:immunology / Q000378:metabolism; D012212:Rheology; D000080506:Sialyl Lewis X Antigen; D014162:Transfection / Q000276:immunology
D000911:Antibodies, Monoclonal; D019040:E-Selectin; D007074:Immunoglobulin G; D007376:Interleukin-2; D007983:Lewis Blood Group Antigens; D008562:Membrane Glycoproteins; D009844:Oligosaccharides; D019007:P-Selectin; C084882:P-selectin ligand protein; D000080506:Sialyl Lewis X Antigen; D019041:L-Selectin; D018664:Interleukin-12