[Solid-phase immunoenzyme ass for detecting Giardia lamblia antigens].
An ELISA sandwich for the detection of Giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from G. lamblia. The sensitivity of the technique was 94.8% and the specificity 98.3%. The method detects an antigen concentration of 31 ng. The procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.
Instituto de Medicina Tropical Pedro Kourí.
D
D
Torres
M
M
Fernández
T
T
Brito
C
C
Finlay
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis / Q000469:parasitology; D006801:Humans; D011817:Rabbits; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan