Down-regulation of protein kinase C isoforms in irritant contact dermatitis.
Protein kinase C (PKC) isoforms are important in cell signal transduction associated with regulation of cell proliferation and differentiation. In this study, alterations of PKC isoform levels in irritant patch test reactions were detected by Western immunoblots. 4 chemically and structurally different irritants, 4% and 8% hydrochloric acid (HCl); 1% and 2% sodium hydroxide (NaOH); 20% and 40% nonanoic acid (NON) in 1-propanol; and 5% and 10% sodium dodecyl sulfate (SDS) were applied on the backs of mice in Finn Chambers and fixed with surgical dressings. The patches were kept on the skin for 24 h and removed. 24 h after removal, mild erythema with thickening of skin without vesicles was observed in all irritated skin, except that 4% HCl and 1% NaOH treated skin showed unremarkable skin reaction. No visible skin reaction was detected in vehicle-treated skin. PKC isoform alpha, beta, gamma, and delta levels in irritated skin revealed a 10% to 65% decrease compared to vehicle treated skin. These results indicate that in HCl, NaOH, NON and SDS-induced irritation, activation of the PKC related cell signal transduction cascade may be involved, and that PKC mediated events may be a common phenomenon in irritant contact dermatitis.
Department of Dermatology, University of Illinois at Chicago, 60612, USA.
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Li
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Fiedler
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Kumar
D000818:Animals; D017449:Dermatitis, Allergic Contact / Q000201:enzymology* / Q000209:etiology; D017453:Dermatitis, Irritant / Q000201:enzymology* / Q000209:etiology; D015536:Down-Regulation; D004789:Enzyme Activation / Q000187:drug effects; D004890:Erythema / Q000139:chemically induced / Q000503:physiopathology; D005260:Female; D007527:Isoenzymes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010328:Patch Tests; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism*; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta; D012867:Skin / Q000187:drug effects / Q000201:enzymology / Q000503:physiopathology
D007527:Isoenzymes; C497259:Prkcd protein, mouse; C087857:protein kinase C gamma; C496630:Prkca protein, mouse; D011493:Protein Kinase C; D064546:Protein Kinase C beta; D051571:Protein Kinase C-alpha; D051745:Protein Kinase C-delta