Contrast-enhancement for the image of human immunodeficiency virus from ultrathin section by immuno electron microscopy.
A simple contrast-enhancement method is described for electron microscopic imaging of the human immunodeficiency virus (HIV) from a sample embedded in Lowicryl K4M resin, by immuneelectron microscopy. Ultrathin sections were treated with a mixture of ruthenium red dye (RR) and osmium tetroxide (OSO4). This treatment provided good contrast enhancement of the entire ultrastructural image of virus particles without the loss of immunolabelling. RR/OsO4 solution is simple to prepare and provides a better contrast than that which is achieved during conventional post-embedding immunoelectron microscopy. Treatment of ultrathin sections from low temperature-embedded samples with RR/OsO4 solution is recommended.
Kohno
T
T
Department of Microbiology, Osaka Medical College, Japan.
Fujioka
Y
Y
Goto
T
T
Morimatsu
S
S
Morita
C
C
Nakano
T
T
Sano
K
K
D002460:Cell Line; D015498:HIV-2 / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007150:Immunohistochemistry; D016253:Microscopy, Immunoelectron / Q000379:methods*; D009993:Osmium Tetroxide; D012116:Resins, Plant; D012430:Ruthenium Red; D013601:T-Lymphocytes
D012116:Resins, Plant; D012430:Ruthenium Red; D009993:Osmium Tetroxide