Effects of thyroid hormone on carbonic anhydrase I gene expression in human erythroid cells.
Individuals with hyperthyroidism exhibit concentrations of carbonic anhydrase I (CAI) in red blood cells that reflect the integrated serum thyroid hormone concentration over the preceding few months. Furthermore, triiodothyronine T3, at a physiological free concentration, decreases the CAI concentration in both human erythroleukemic YN-1 cells and burst-forming unit-erythroid (BFU-E)-derived cells. In the present study, the effect of T3 on CAI mRNA levels in various human erythroleukemic cell lines (YN-1, HEL and KU-812) and BFU-E-derived cells was studied. Northern analysis of RNA extracted from erythroid cells revealed a CAI mRNA of 1.5 kilobases. T3 significantly decreased the levels of CAI mRNA in YN-1 and BFU-E-derived cells in a dose-dependent manner. Incubation of T3-stimulated cells with actinomycin D prevented the decrease in CAI mRNA levels. By contrast, T3 had no effect on either the concentrations of CAI or the levels of CAI mRNA in HEL and KU-812 cells. These results suggest that YN-1 and BFU-E-derived cells may be useful models for investigating T3 actions on CAI mRNA in human cells.
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
H
H
Hori
K
K
Yoshida
H
H
Fukazawa
Y
Y
Kiso
N
N
Sayama
K
K
Mori
Y
Y
Aizawa
J I
JI
Tani
S
S
Ito
D002256:Carbonic Anhydrases / Q000235:genetics*; D015672:Erythroid Precursor Cells / Q000378:metabolism / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D012333:RNA, Messenger; D014284:Triiodothyronine; D002256:Carbonic Anhydrases