Sex-chromosome elimination in the bandicoot Isoodon macrourus using Y-linked markers.
Cytogenetic studies have shown that the Y chromosome is eliminated from many somatic cell types of the bandicoot Isoodon macrourus, an Australian marsupial. Molecular techniques allow examination of a greater range of tissue types than that possible using cytogenetic techniques. The presence or absence of the Y chromosome was established using partial sequences of the Y-linked SRY and UBE1Y genes in I. macrourus, with the X-linked gene G6PD as a control. We show that a very small proportion of cells comprising hematopoietic tissues, and even fewer cells in peripheral blood, retain the Y chromosome. The Y chromosome is retained in most brain, liver, kidney, and lung cells and in cardiac and skeletal muscle. We also show that the bandicoot Y chromosome is retained in some cell types within tissues previously believed to completely eliminate the Y chromosome.
School of Biological Sciences, Macquarie University, NSW (Australia).
C M
CM
Watson
S H
SH
Margan
P G
PG
Johnston
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002869:Chromosome Aberrations / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D005819:Genetic Markers / Q000235:genetics; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007621:Karyotyping; D008025:Ligases / Q000235:genetics / Q000378:metabolism; D008297:Male; D008394:Marsupialia / Q000235:genetics*; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012730:Sex Chromosomes / Q000235:genetics*; D051876:Sex-Determining Region Y Protein; D014018:Tissue Distribution; D014157:Transcription Factors; D044767:Ubiquitin-Protein Ligases; D014960:X Chromosome / Q000201:enzymology / Q000235:genetics; D014998:Y Chromosome / Q000737:chemistry / Q000235:genetics*
D004268:DNA-Binding Proteins; D005819:Genetic Markers; D009687:Nuclear Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors; D004247:DNA; D005954:Glucosephosphate Dehydrogenase; D044767:Ubiquitin-Protein Ligases; D008025:Ligases