Solvent exchange rates of side-chain amide protons in proteins.
Solvent exchange rates and temperature coefficients for Asn/Gln side-chain amide protons have been measured in Escherichia coli HPr. The protons of the eight side-chain amide groups (two Asn and six Gln) exhibit varying exchange rates which are slower than some of the fast exchanging backbone amide protons. Differences in exchange rates of the E and Z protons of the same side-chain amide group are obtained by measuring exchange rates at pH values > 8. An NOE between a side-chain amide proton and a bound water molecule was also observed.
Department of Biochemistry, University of Washington, Seattle 98195-7742, USA.
P
P
Rajagopal
B E
BE
Jones
R E
RE
Klevit
D001426:Bacterial Proteins / Q000737:chemistry; D004926:Escherichia coli; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry*; D011522:Protons
D001426:Bacterial Proteins; D011522:Protons; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C030182:phosphocarrier protein HPr