Isolation and characterization of S-layer proteins from a vent prosthecate bacterium.
MWapp 116,000 and 29,000 proteins (p116 and p29), major outer membrane proteins of Hyphomonas jannaschiana reproductive cells, were extracted from cell envelopes by dialysis against EDTA, 2 M urea or distilled water. These proteins were precipitated by divalent cations and resolubilized by EDTA-Na, reflecting alternate monomer, multimer states. From two-dimensional gel electrophoresis it was determined that p116 and p29 had a pl of 4.5. Both were glycoproteins. Results suggest that p116 and p29 are surface layer (S-layer) proteins, with p116 a tetramer of the p29. The S-layer could protect the adherent H. jannaschiana reproductive cell from exoenzyme activity, antibiotics and other bacteriocidal molecules produced in the bacterial films formed on many marine surfaces.
Department of Microbiology, University of Maryland, College Park 20742, USA.
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Weiner
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000648:ultrastructure; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D008970:Molecular Weight; D010140:Pacific Ocean
D001425:Bacterial Outer Membrane Proteins; D006023:Glycoproteins