Endothelin-1-like immunoreactivity in a new rodent model of spontaneous hypertension.
The purpose of this study was to determine plasma and tissue endothelin-1 (ET-1)-like immunoreactivity in a new rodent model of spontaneous hypertension. Plasma and tissues were procured from pentobarbital-anesthetized 16- to 18-week-old male hamsters with spontaneous hypertension and genetically/age-matched normotensive hamsters. We found that ET-1-like immunoreactivity in the plasma was similar in both groups. However, renal and cardiac ET-1-like immunoreactivity was 11- and 1.7-fold higher in spontaneously hypertensive hamsters relative to normotensive hamsters, respectively (P < .05). ET-1-like immunoreactivity was slightly, but significantly, lower in the lung and spleen of spontaneously hypertensive hamsters relative to normotensive hamsters (P < .05). ET-1-like immunoreactivity in the liver and brain was similar in both groups. We conclude that ET-1-like immunoreactivity is significantly higher in two target organs for hypertension, kidney and heart, but not in plasma or brain of adult male hamsters with spontaneous hypertension, relative to genetically/age-matched normotensive hamsters. We suggest that renal and cardiac ET-1 could play a role in the natural history of spontaneous hypertension in hamsters.
Department of Pharmaceutics and Pharmacodynamics, University of Illinois at Chicago, 60612-7323, USA.
A
A
Gulati
J E
JE
Artwohl
A
A
Kumar
X P
XP
Gao
I
I
Rubinstein
D000818:Animals; D001921:Brain / Q000378:metabolism; D006224:Cricetinae; D004195:Disease Models, Animal; D019332:Endothelin-1 / Q000097:blood*; D006973:Hypertension / Q000097:blood*; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D009206:Myocardium / Q000378:metabolism; D011863:Radioimmunoassay; D013154:Spleen / Q000378:metabolism; D014018:Tissue Distribution
D019332:Endothelin-1