Culture substrate-specific expression of P2Y2 receptors in distal lung epithelial cells isolated from foetal rats.
ATP and UTP did not evoke [Ca2+]i signals in rat foetal lung epithelial cells grown on glass but elicited clear responses in cells grown into functionally polarised epithelia on permeable supports. Moreover, P2Y2 receptor mRNA could not be detected in cells on glass by the polymerase chain reaction but this mRNA species was clearly expressed by polarised cells. P2Y2 receptor expression thus appears to be a feature of the polarised phenotype.
Clunes
M T
MT
Department of Child Health, Ninewells Hospital and Medical School, University of Dundee.
Collett
A
A
Baines
D L
DL
Bovell
D L
DL
Murphie
H
H
Inglis
S K
SK
McAlroy
H L
HL
Olver
R E
RE
Wilson
S M
SM
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D016764:Cell Polarity; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology; D005333:Fetus / Q000378:metabolism; D005898:Glass; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000196:embryology / Q000378:metabolism*; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000096:biosynthesis*; D058473:Receptors, Purinergic P2Y2; D014544:Uridine Triphosphate / Q000494:pharmacology
C546387:P2ry2 protein, rat; D018048:Receptors, Purinergic P2; D058473:Receptors, Purinergic P2Y2; D000255:Adenosine Triphosphate; D002118:Calcium; D014544:Uridine Triphosphate