A method for the 32P labeling of peptides or peptide nucleic acid oligomers.
A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.
Kozlov
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The Salk Institute for Biological Studies, P.O. Box 85800, San Diego, California 92186, USA.
Nielsen
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Orgel
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D000255:Adenosine Triphosphate / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D011088:DNA Ligases / Q000737:chemistry; D007553:Isotope Labeling / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D010761:Phosphorus Radioisotopes / Q000737:chemistry*; D010766:Phosphorylation
D009841:Oligonucleotides; D010455:Peptides; D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D011088:DNA Ligases