Glutathione S-transferase Mu (GST Mu) deficiency and DNA adducts in lymphocytes of smokers.
The effect of smoking on DNA adduct formation in lymphocytes was analysed in individuals with low (deficient) and high (non-deficient) glutathione S-transferase (class Mu) activity. DNA adduct levels in lymphocytes were determined by the highly sensitive nuclease P1-enhanced 32P-postlabeling assay. The lymphocyte DNA adducts/10(8) nucleotides of smokers deficient in glutathione S-transferase Mu activity (n = 12) were significantly higher than those of smokers non-deficient (n = 9) in glutathione S-transferase Mu activity. The DNA adduct levels of the lymphocytes inversely correlated with glutathione S-transferase Mu activity. A correlation was found between DNA adduct levels and daily cigarette consumption. Results of the present study suggest that individuals deficient in glutathione S-transferase Mu activity may be at greater risk of DNA damage.
Food and Drug Toxicology Research Centre, National Institute of Nutrition, Indian Council of Medical Research, Hyderabad.
M
M
Soni
M
M
Madurantakan
K
K
Krishnaswamy
D000328:Adult; D001345:Autoradiography; D018736:DNA Adducts / Q000032:analysis / Q000096:biosynthesis*; D004249:DNA Damage / Q000235:genetics; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000172:deficiency*; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010761:Phosphorus Radioisotopes; D012907:Smoking / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology; D014028:Tobacco Smoke Pollution / Q000009:adverse effects; D018675:Toxicity Tests
D018736:DNA Adducts; D010761:Phosphorus Radioisotopes; D013267:Stilbenes; D014028:Tobacco Smoke Pollution; D005982:Glutathione Transferase; C025906:stilbene oxide