On the origin of membrane vesicles in gram-negative bacteria.
It is proposed that the genesis of extracellular membrane vesicles in Gram-negative bacteria is a result of cell wall turnover. Peptidoglycan turnover would cause a turgor on the outer membrane, causing the outer membrane to bulge and finally bleb. Mechanical motion would then shear the blebs into the culture medium.
Zhou
L
L
Department of Microbiology and Immunology, University of Louisville, KY 40292, USA.
Srisatjaluk
R
R
Justus
D E
DE
Doyle
R J
RJ
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D009112:Muramic Acids / Q000032:analysis; D010455:Peptides / Q000032:analysis; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins; D009112:Muramic Acids; D010455:Peptides; D011506:Proteins