Boundary formation in Drosophila wing: Notch activity attenuated by the POU protein Nubbin.
Cell interactions mediated by Notch-family receptors have been implicated in the specification of tissue boundaries in vertebrate and insect development. Although Notch ligands are often widely expressed, tightly localized activation of Notch is critical for the formation of sharp boundaries. Evidence is presented here that the POU domain protein Nubbin contributes to the formation of a sharp dorsoventral boundary in the Drosophila wing. Nubbin represses Notch-dependent target genes and sets a threshold for Notch activity that defines the spatial domain of boundary-specific gene expression.
Neumann
C J
CJ
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
Cohen
S M
SM
D000818:Animals; D002135:Calcium-Binding Proteins; D004330:Drosophila / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008024:Ligands; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D009154:Mutation; D017351:N-Acetylglucosaminyltransferases; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology; D050809:POU Domain Factors; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology; D051880:Receptors, Notch; D000072099:Serrate-Jagged Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014921:Wings, Animal / Q000254:growth & development* / Q000378:metabolism; D051155:Wnt1 Protein
D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D008024:Ligands; D008565:Membrane Proteins; C497759:N protein, Drosophila; D009687:Nuclear Proteins; D050809:POU Domain Factors; D011518:Proto-Oncogene Proteins; D051880:Receptors, Notch; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; D051155:Wnt1 Protein; C064045:delta protein; C104121:nub protein, Drosophila; C095064:vg protein, Drosophila; C074622:wg protein, Drosophila; D017351:N-Acetylglucosaminyltransferases; C090576:fng protein, Drosophila