New gene assignments using a complete, characterized sheep-hamster somatic cell hybrid panel.
The generation and characterization of new sheep-hamster cell hybrids is reported from the fusion of sheep white blood cells with six different hamster auxotrophs. Selection from these and previously generated cell hybrids has led to the production of a panel of 30 hybrids covering the complete sheep genome of 28 chromosomes. Over half of the cell hybrids in this panel contain single sheep chromosomes. By complementation, the following new assignments have been made using the panel: phosphoribosyl N-formylglycinamide amidotransferase (PRFGA) to sheep chromosome (chr) 11; adenylosuccinate synthetase (ADSS) to sheep chr 12; adenylosuccinate lyase (ADSL) to sheep chr 3q; 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGCS) to sheep chr 16; dihydrofolate reductase (DHFR) to sheep chr 5; and adenine phosphoribosyltransferase (APRT) to sheep chr 14. The gene phosphoribosylaminoinidazole-carboxamide formyltransferase/Inosinicase (PRACFT) has now been regionally assigned to chr 2q. By isozyme analysis, phosphogluconate dehydrogenase (PGD) was assigned to sheep chr 12, anchoring the sheep syntenic group U1 to this chromosome, and mannose phosphate isomerase (MPI) was assigned to sheep chr 18. Furthermore, the chromosomal assignment of 110 microsatellites was confirmed using this cell panel.
Burkin
D J
DJ
AgResearch, Department of Biochemistry, Dunedin, New Zealand.
Broad
T E
TE
Lambeth
M R
MR
Burkin
H R
HR
Jones
C
C
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics; D000264:Adenylosuccinate Lyase / Q000235:genetics; D000265:Adenylosuccinate Synthase / Q000235:genetics; D000818:Animals; D002417:Cattle; D002871:Chromosome Banding / Q000662:veterinary; D002874:Chromosome Mapping / Q000662:veterinary*; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005816:Genetic Complementation Test / Q000662:veterinary; D016678:Genome; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D019877:Hydroxymethyl and Formyl Transferases / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007527:Isoenzymes / Q000032:analysis* / Q000235:genetics; D007962:Leukocytes / Q000737:chemistry; D008359:Mannose-6-Phosphate Isomerase / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D007652:Oxo-Acid-Lyases / Q000235:genetics; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D051026:Phosphoribosylglycinamide Formyltransferase; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep / Q000235:genetics*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D007527:Isoenzymes; D010734:Phosphogluconate Dehydrogenase; D013762:Tetrahydrofolate Dehydrogenase; D019877:Hydroxymethyl and Formyl Transferases; D051026:Phosphoribosylglycinamide Formyltransferase; D051027:Phosphoribosylaminoimidazolecarboxamide Formyltransferase; D000228:Adenine Phosphoribosyltransferase; D007652:Oxo-Acid-Lyases; C021410:3-hydroxy-3-methylglutaryl-coenzyme A lyase; D000264:Adenylosuccinate Lyase; D008359:Mannose-6-Phosphate Isomerase; D000265:Adenylosuccinate Synthase