Effects of gamma-glutamylcysteine ethyl ester on heart mitochondrial creatine kinase activity: involvement of sulfhydryl groups.
To study the mechanism of the protective effect of gamma-glutamylcysteine ethyl ester, mitochondrial creatine kinase activity of rat heart was measured. Gamma-glutamylcysteine ethyl ester had a protective effect against the depression of creatine kinase activity induced by xanthine + xanthine oxidase or hydrogen peroxide. Gamma-glutamylcysteine ethyl ester also prevented the depression of creatine kinase activity induced by N-ethylmaleimide. It is suggested that the protective effect of gamma-glutamylcysteine ethyl ester is related to oxygen free radicals or to reduction of the sulfhydryl groups of the enzyme which were previously oxidized by oxygen free radicals.
Photon Medical Research Center, Hamamatsu University School of Medicine, Japan. hide289@hama-med.ac.jp
H
H
Hayashi
M
M
Iimuro
Y
Y
Matsumoto
M
M
Kaneko
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism*; D004151:Dipeptides / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000201:enzymology*; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000502:physiology*; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism
D004151:Dipeptides; D004791:Enzyme Inhibitors; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D013481:Superoxides; C067744:N-gamma-glutamylcysteine ethyl ester; D003402:Creatine Kinase; D005033:Ethylmaleimide