Yeast permeabilization as a tool for measurement of in situ enzyme activity: localization of alkaline phosphatase.
The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.
Spasova
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Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
Galabova
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D000469:Alkaline Phosphatase / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002848:Chromatography, DEAE-Cellulose; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D017830:Octoxynol; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014617:Vacuoles / Q000201:enzymology
D017830:Octoxynol; D000469:Alkaline Phosphatase