A pseudoautosomal random amplified polymorphic DNA marker for the sex chromosomes of Silene dioica.
The segregation pattern of an 810-bp random amplified polymorphic DNA (RAPD) band in the F1 and backcross generations of a Silene dioica (L.) Clairv. family provides evidence that this molecular marker is located in the pseudoautosomal region (PAR) of the X and Y chromosomes. The marker was found through a combination of bulked segregant analysis (BSA) and RAPD techniques. Recombination rates between this pseudoautosomal marker and the differentiating portion of the Y chromosome are 15% in both generations. Alternative explanations involving nondisjunction or autosomal inheritance are presented and discussed. Chromosome counts provide evidence against the nondisjunction hypothesis, and probability calculations argue against the possibility of autosomal inheritance. This constitutes the first report of a pseudoautosomal DNA marker for plant sex chromosomes.
Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA. distilio@bio.umass.edu
V S
VS
Di Stilio
R V
RV
Kesseli
D L
DL
Mulcahy
D001483:Base Sequence; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D005787:Gene Frequency; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D019684:Magnoliopsida / Q000235:genetics*; D019105:Random Amplified Polymorphic DNA Technique; D012730:Sex Chromosomes / Q000235:genetics
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers