Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves.
The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-alpha protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-alpha proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected.
Central Research Institute of Electric Power Industry, Chiba, Japan.
K
K
Shoji
T
T
Iwasaki
R
R
Matsuki
M
M
Miyao
N
N
Yamamoto
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D015536:Down-Regulation / Q000235:genetics* / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D017343:Genes, Plant / Q000528:radiation effects*; D006801:Humans; D008027:Light; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics* / Q000528:radiation effects; D010788:Photosynthesis / Q000235:genetics; D018515:Plant Leaves / Q000235:genetics* / Q000528:radiation effects; D010944:Plants / Q000235:genetics; D017422:Sequence Analysis, DNA; D028901:alpha Karyopherins
D018076:DNA, Complementary; C490630:KPNA2 protein, human; D009687:Nuclear Proteins; D028901:alpha Karyopherins