Phytochemical study and antiinflammatory properties of Lobelia laxiflora L.
Three new piperidine alkaloids were isolated from stems, leaves and flowers of Lobelia laxiflora L. (Campanulaceae). The structures of racem. cis-8,10-diethyl-3,4-dehydrolobelidiol (1), racem. trans-8-ethyl-10-phenyl-3,4-dehydrolobelidiol (2) and racem. cis-8-ethyl-10-phenyl-3, 4-dehydrolobelidiol (3) were established by spectral analyses. The residues obtained from the ethanol extracts from stems (S), leaves (L), and flowers (F) were applied in carrageenan (Car)- and cobra venom (CV)-induced acute inflammation in mice. A suppression of paw edema formation at a dose of 100 mg kg-1 was established. In this study the antiinflammatory potential of Lobelia l. was regarded in connection with the complement system. The sequential activation and assembly into functional units of complement components can proceed via two different pathways, named classical (CP) and alternative (AP). The ability of the residues, nonalkaloid fractions, alkaloid fractions and the three alkaloids at a concentration from 0.125 to 1.0 mg ml-1 to inhibit complement activation and thus to prevent inflammatory process was estimated in vitro in human serum via both pathways. All of them inhibited complement activity with a predominant action on CP.
Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria. PHILIPOV@BGUCT.ACAD.BG
S
S
Philipov
R
R
Istatkova
N
N
Ivanovska
P
P
Denkova
K
K
Tosheva
H
H
Navas
J
J
Villegas
D000470:Alkaloids / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002351:Carrageenan; D003167:Complement Activation / Q000187:drug effects; D004546:Elapid Venoms; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy*; D019684:Magnoliopsida; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D018547:Plant Stems; D010946:Plants, Medicinal
D000470:Alkaloids; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004546:Elapid Venoms; D010936:Plant Extracts; D002351:Carrageenan