Correlation of specific antibody titre and avidity with protection in cattle immunized against Fasciola hepatica.
Cattle produce specific serum antibody mainly of the IgG1 isotype in response to infection with the liver fluke, Fasciola hepatica. In these animals a positive correlation between fluke-specific serum IgG1 levels and fluke-burden in non-immunized infected animals was observed. In contrast, immunization of cattle with a combination of the fluke-derived antigens cathepsin L2 (CL2) and fluke haemoglobin (FHb) in Freund's complete/incomplete adjuvant (FCA/FLA) induced a specific antibody response involving IgG2, as well as IgG1. These immunized animals also exhibited very high (72%) levels of protection against a subsequent challenge infection. When the vaccine was administered in FIA alone the specific antibody response, while still involving IgG1 and IgG2, was of lower magnitude (10-fold and 100-fold, respectively) and no significant reduction in fluke burden was observed following challenge. Nevertheless, in these animals, a strong IgG2 response was associated with low fluke burdens. These results provide further evidence of the non-protective nature of specific immune responses in cattle following F. hepatica infection, and demonstrate that vaccination can induce a qualitatively different, and protective, response.
Department of Veterinary Microbiology and Parasitology, University College Dublin, Ireland. Gracem@vetmed.ucd.ie
G
G
Mulcahy
F
F
O'Connor
S
S
McGonigle
A
A
Dowd
D G
DG
Clery
S J
SJ
Andrews
J P
JP
Dalton
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000915:Antibody Affinity; D000918:Antibody Specificity; D000947:Antigens, Helminth / Q000008:administration & dosage; D002403:Cathepsins / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control*; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D005620:Freund's Adjuvant / Q000008:administration & dosage; D006454:Hemoglobins / Q000276:immunology; D007114:Immunization / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D013997:Time Factors
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D006454:Hemoglobins; D007074:Immunoglobulin G; D005620:Freund's Adjuvant; D002403:Cathepsins