Non-Hodgkin's lymphomas and myeloid disorders: deletions associated with t(2;5) and t(3;5) detected by FISH.
The nucleophosmin (NPM) gene is involved in two recurrent translocations in hematological malignancies: t(2;5) (p23;q35) in anaplastic large cell lymphoma (ALCL) and t(3;5)(q25.1;q34-35) in myelodysplasia and acute non-lymphocytic leukemia (ANLL). Using eight YACs encompassing the 5q34-q35 region, we could easily detect these two translocations. In both types of translocation, probable unexpected deletions were also discovered downstream of the breakpoint at 5q35.
Dragon-Durey
M A
MA
URA 1967 CNRS, Laboratoire de Cytogénétique et de Génétique Oncologiques, Institut Gustave Roussy, Villejuif, France.
Goguel
A F
AF
Leonard
C
C
Berger
R
R
Bressac-de-Paillerets
B
B
Fauvet
D
D
Bernheim
A
A
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic