Transcriptional regulation of expression of the rainbow trout albumin gene by estrogen.
Estrogens modulate the expression of many liver-specific genes in oviparous species. For instance, expression of the estrogen receptor and vitellogenin genes is strongly up-regulated by estradiol in rainbow trout liver. Using hepatocyte primary cultures, we demonstrate that trout albumin (Alb) gene is also regulated by this hormone. Indeed, treatment of hepatocytes with 1 microM estradiol led, after 24 h, to a dramatic decrease in Alb mRNA level. To investigate the mechanism of this down-regulation, run-off experiments were performed and mRNA half-lives were determined in the presence and absence of estradiol. The results show that the down-regulation of Alb mRNA expression by estrogens occurs only at the transcriptional level.
Endocrinologie Moléculaire de la Reproduction, UPRES-A Biologie Cellulaire et Reproduction, INRA, Université de Rennes I, France.
G
G
Flouriot
B
B
Ducouret
L
L
Byrnes
Y
Y
Valotaire
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007700:Kinetics; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D017686:Oncorhynchus mykiss; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D012333:RNA, Messenger; D012709:Serum Albumin; D004958:Estradiol