Purification of endogenous digitalis-like factors from normal human urine.
We detected two candidates for endogenous digitalis-like factors in human urine based on the inhibition of 3H-ouabain binding to human erythrocytes. Two ouabain-displacing compound(ODC)s were consistently eluted off the C18 reverse phase HPLC column with 18% and 31% acetonitrile. The more-polar ODC-1 was ubiquitously found in mammals, markedly increased after acute and chronic salt loading in humans, and was thought to be a natriuretic factor with vasoactive property. ODC-1 mostly resembled ouabain in biological, physicochemical, and chromatographic properties and may correspond to ouabainlike compound purified by other investigators. The less-polar ODC-2 was indistinguishable from digoxin in proton nuclear magnetic resonance(NMR) and fast atom bombardment(FAB) mass spectrum.
Second Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
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Goto
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Yamada
D001685:Biological Factors / Q000302:isolation & purification* / Q000494:pharmacology / Q000652:urine; D002298:Cardenolides; D002851:Chromatography, High Pressure Liquid; D004077:Digoxin; D004791:Enzyme Inhibitors / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D009131:Muscle, Smooth, Vascular / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D012503:Saponins / Q000302:isolation & purification* / Q000652:urine; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014556:Urine / Q000737:chemistry*
D001685:Biological Factors; D002298:Cardenolides; D004791:Enzyme Inhibitors; D012503:Saponins; C034504:digoxin-like factors; D010042:Ouabain; D004077:Digoxin; D000254:Sodium-Potassium-Exchanging ATPase