Role of neutrophil elastase in compression-induced spinal cord injury in rats.
We have previously demonstrated the importance of activated neutrophils in compression-induced spinal cord injury (SCI) in rats. In the present study, we investigate the action of neutrophil elastase in posttraumatic SCI, using two neutrophil elastase inhibitors (Eglin C and L658,758). SCI was induced by applying a 20-g weight to the spinal cord for 20 min at the level of T12, resulting in hindlimbs motor disturbances, which, when evaluated using a inclined-plane test, were significantly attenuated by Eglin C or L658,758. Histologic examination revealed that intramedullary hemorrhages observed 24 h after trauma were markedly attenuated in these agents. These inhibitors also significantly decreased neutrophil accumulation as shown by myeloperoxidase activity in the damaged spinal cord segment. Induction of leukocytopenia had the same effects as Eglin C or L658,758. These findings implicated neutrophil elastase in SCI. The enzyme may induce vascular damage leading to spinal cord ischemia.
Taoka
Y
Y
Department of Laboratory Medicine, Kumamoto University Medical School, 1-1-1 Honjo, Kumamoto 860, Japan.
Okajima
K
K
Murakami
K
K
Johno
M
M
Naruo
M
M
D000818:Animals; D002511:Cephalosporins / Q000494:pharmacology; D019272:Leukocyte Elastase / Q000502:physiology*; D007970:Leukopenia / Q000139:chemically induced / Q000503:physiopathology; D008297:Male; D008466:Mechlorethamine / Q000494:pharmacology; D009195:Peroxidase / Q000378:metabolism; D011506:Proteins; D051381:Rats; D017208:Rats, Wistar; D015843:Serpins / Q000494:pharmacology; D013116:Spinal Cord / Q000201:enzymology / Q000473:pathology; D013117:Spinal Cord Compression / Q000150:complications*; D013119:Spinal Cord Injuries / Q000209:etiology* / Q000473:pathology / Q000503:physiopathology
D002511:Cephalosporins; D011506:Proteins; D015843:Serpins; C028656:eglin proteinase inhibitors; C065332:L 658758; D008466:Mechlorethamine; D009195:Peroxidase; D019272:Leukocyte Elastase