Establishment of chimerism in donor liver with recipient-type bone marrow cells prior to liver transplantation produces marked suppression of allograft rejection in rats.
In this study, we investigated whether establishment of chimerism in donor liver with recipient-type bone marrow cells (BMCs) prior to liver transplantation could prolong the liver allograft survival. Donor female ACI rats were inoculated with recipient-type BMCs of male LEW rats via the portal vein, with or without irradiation as cytoablation, followed by intramuscular administration of FK506 for 5 days. At 1-2 months later, livers were harvested and transplanted into naive female LEW rats. No immunosuppressants were used. Chimerism in donor rats was confirmed by primers specific for the sex determinant Y chromosome of rats. With livers from rats pretreated with recipient-type BMCs, survival of liver allografts was significantly extended, irrespective of irradiation. These results showed that modification of the donor liver by intraportal injection of recipient-type BMCs and concomitant administration of FK506 prior to liver transplantation prolonged liver allograft survival in rats.
Sanada
O
O
Second Department of Surgery, School of Medicine, Hiroshima University, Japan.
Fukuda
Y
Y
Sumimoto
R
R
Hoshino
S
S
Nishihara
M
M
Kaneda
K
K
Asahara
T
T
Dohi
K
K
D000818:Animals; D016026:Bone Marrow Transplantation; D003713:Dendritic Cells; D005260:Female; D006084:Graft Rejection / Q000517:prevention & control*; D006085:Graft Survival; D016031:Liver Transplantation; D008297:Male; D011300:Preoperative Care; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D015996:Survival Rate; D018183:Transplantation Chimera