Trematode hemoglobins show exceptionally high oxygen affinity.
Ligand binding studies were made with hemoglobin (Hb) isolated from trematode species Gastrothylax crumenifer (Gc), Paramphistomum epiclitum (Pe), Explanatum explanatum (Ee), parasitic worms of water buffalo Bubalus bubalis, and Isoparorchis hypselobagri (Ih) parasitic in the catfish Wallago attu. The kinetics of oxygen and carbon monoxide binding show very fast association rates. Whereas oxygen can be displaced on a millisecond time scale from human Hb at 25 degrees C, the dissociation of oxygen from trematode Hb may require a few seconds to over 20 s (for Hb Pe). Carbon monoxide dissociation is faster, however, than for other monomeric hemoglobins or myoglobins. Trematode hemoglobins also show a reduced rate of autoxidation; the oxy form is not readily oxidized by potassium ferricyanide, indicating that only the deoxy form reacts rapidly with this oxidizing agent. Unlike most vertebrate Hbs, the trematodes have a tyrosine residue at position E7 instead of the usual distal histidine. As for Hb Ascaris, which also displays a high oxygen affinity, the trematodes have a tyrosine in position B10; two H-bonds to the oxygen molecule are thought to be responsible for the very high oxygen affinity. The trematode hemoglobins display a combination of high association rates and very low dissociation rates, resulting in some of the highest oxygen affinities ever observed.
Kiger
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L
INSERM U473, 94276 Le Kremlin Bicêtre Cedex, France.
Rashid
A K
AK
Griffon
N
N
Haque
M
M
Moens
L
L
Gibson
Q H
QH
Poyart
C
C
Marden
M C
MC
D000818:Animals; D001048:Aplysia; D001200:Ascaris; D002020:Buffaloes / Q000469:parasitology; D002263:Carboxyhemoglobin / Q000378:metabolism; D002397:Catfishes / Q000469:parasitology; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009211:Myoglobin / Q000378:metabolism; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism*; D013045:Species Specificity; D014200:Trematoda / Q000502:physiology*
D006454:Hemoglobins; D009211:Myoglobin; D010108:Oxyhemoglobins; C018013:carboxymyoglobin; C017595:oxymyoglobin; D002263:Carboxyhemoglobin