Combining MALDI mass spectrometry and biomolecular interaction analysis using a biomolecular interaction analysis instrument.
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been combined with biomolecular interaction analysis (BIA) in a Biacore instrument. A method has been developed for the recovery of the affinity-bound molecules from the sensor chip in a few microliters ready for mass spectrometric analysis. The procedure is illustrated with two molecular systems which exemplify antibody-antigen and DNA-protein interactions. In both cases, femtomole quantities of the affinity-bound proteins were eluted and subsequently detected by MALDI-MS. Whereas the Biacore analysis yields the surface concentration of protein bound to the sensor chip, identity of the bound compounds is revealed in the second step by accurate molecular mass determination. Combining the information of the two analyses allows calculation of the total surface molar concentration of affinity-bound molecules.
Department of Molecular Biology, Odense University, Denmark.
C P
CP
Sönksen
E
E
Nordhoff
O
O
Jansson
M
M
Malmqvist
P
P
Roepstorff
D000937:Antigen-Antibody Reactions; D001426:Bacterial Proteins / Q000032:analysis; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis; D015342:DNA Probes / Q000138:chemical synthesis; D004268:DNA-Binding Proteins / Q000032:analysis; D006801:Humans; D009211:Myoglobin / Q000032:analysis; D012097:Repressor Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D001426:Bacterial Proteins; D015342:DNA Probes; D004268:DNA-Binding Proteins; D009211:Myoglobin; C086169:ParR protein, bacteria; D012097:Repressor Proteins; D004247:DNA