Brassinolides promote the expression of a new Cicer arietinum beta-tubulin gene involved in the epicotyl elongation.
A cDNA clone, CanTUB, encoding a putative beta-tubulin protein was isolated from a cDNA library constructed from 5-day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant beta-tubulins. Putative sequences for autoregulation and tubulin mRNA stability, GTP and Ca2+/MAPs (microtubule-associated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple beta-tubulin genes. The level of expression of beta-tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of beta-tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolide-induced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for beta-tubulin.
Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Salamanca, Spain.
F J
FJ
Muñoz
E
E
Labrador
B
B
Dopico
D060406:Brassinosteroids; D002777:Cholestanols / Q000494:pharmacology*; D003001:Cloning, Molecular; D007887:Fabaceae / Q000235:genetics*; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010946:Plants, Medicinal; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013260:Steroids, Heterocyclic / Q000494:pharmacology*; D014404:Tubulin / Q000235:genetics*
D060406:Brassinosteroids; D002777:Cholestanols; D010937:Plant Growth Regulators; D012333:RNA, Messenger; D018749:RNA, Plant; D013260:Steroids, Heterocyclic; D014404:Tubulin; C023623:brassinolide