Case report: high fertilization rate in conventional in-vitro fertilization utilizing spermatozoa from an oligozoospermic subject presenting microdeletions of the Y chromosome long arm.
A case is reported in which a high fertilization rate was achieved by conventional in-vitro fertilization (IVF), using spermatozoa from an oligozoospermic man carrying a microdeletion of the long arm of the Y chromosome. The patient presented with idiopathic infertility of 10 years duration; the fertility status of his wife was completely normal. After IVF, five out of eight oocytes retrieved showed normal fertilization and four showed normal embryo cleavage. Four embryos were transferred; however, pregnancy did not result. These results demonstrate that spermatozoa from oligozoospermic patients carrying a Yq microdeletion are fully competent in achieving capacitation, acrosome reaction and fertilizing ability during IVF. Therefore, although definitive conclusions cannot be made from a single case report, we suggest that Yq microdeletion analysis should be considered in oligozoospermic patients undergoing conventional IVF.
Rossato
M
M
Clinica Medica III, University of Padova, Italy.
Ferlin
A
A
Garolla
A
A
Pistorello
M
M
Foresta
C
C
D000328:Adult; D002872:Chromosome Deletion; D004252:DNA Mutational Analysis; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000235:genetics / Q000503:physiopathology*; D012729:Sex Chromosome Aberrations / Q000235:genetics / Q000503:physiopathology*; D013094:Spermatozoa / Q000502:physiology; D014998:Y Chromosome / Q000235:genetics* / Q000648:ultrastructure