Solid-phase extraction and liquid chromatographic quantitation of the antiarrhythmic drug L-768673 in a microemulsion formulation.
A sensitive and specific method based in solid-phase extraction and reverse-phase liquid chromatography was developed and validated for the quantitation of L-768673 in a microemulsion formulation. Following a water wash, the drug was eluted from the extraction column with acetonitrile and was analyzed on a reverse-phase C18 column with UV detection at 245 nm. The mobile phase consisted of acetonitrile-0.2% trifluoroacetic acid, 0.1% triethylamine (53:47 v/v). The retention time L-768673 was approximately 28 min with a flow rate of 1.5 ml min-1.
Pharmaceutical Research and Development Department, Merck Research Laboratories, West Point, Pa 19486, USA.
R R
RR
Kenney
R J
RJ
Forsyth
H
H
Jahansouz
D000081:Acetamides / Q000032:analysis*; D000889:Anti-Arrhythmia Agents / Q000032:analysis*; D001570:Benzodiazepinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004655:Emulsions; D010919:Placebos; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000081:Acetamides; D000889:Anti-Arrhythmia Agents; D001570:Benzodiazepinones; D004655:Emulsions; C109598:L 768673; D010919:Placebos