Note: purification and characterization of the bacteriocin PsVP-10 produced by Pseudomonas sp.
A bacteriocin (bacteriocin PsVP-10) produced by Pseudomonas sp. R-10 was purified by a simple method that included an extraction of the bacteriocin with chloroform, followed by cation exchange chromatography. The purity of the bacteriocin was verified by RP-HPLC. It is a peptide of 2.4 kDa, very stable to heat, to proteolytic enzymes and to pH. It presents a very broad spectrum of antimicrobial activity against Gram-positive and Gram-negative bacteria.
Department of Biological Sciences, University of Talca, Chile.
E
E
Hubert
O
O
Lobos
P
P
Brevis
C
C
Padilla
D000596:Amino Acids / Q000032:analysis; D001430:Bacteriocins / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008826:Microbial Sensitivity Tests; D011549:Pseudomonas / Q000378:metabolism*
D000596:Amino Acids; D001430:Bacteriocins