Decay-accelerating factor (CD55) deficiency phenotypes in Japanese.
Decay-accelerating factor (DAF, CD55) is a complement regulatory glycoprotein that expresses the Cromer-system blood group antigens. Two, very rare, inherited DAF-deficiency phenotypes, Inab and Dr(a-), were identified in Japanese propositi. Red cells of the Inab phenotype propositus had no Cromer-system antigens and did not bind monoclonal anti-DAF. The Inab propositus was homozygous for a DAF non-sense mutation, converting the Trp53 codon to a stop codon; her parents were heterozygous for this mutation. This is the same mutation as that previously found in the original Inab phenotype propositus. Haemagglutination-inhibition titrations of the serum of the Inab propositus with soluble-recombinant DAF demonstrated that anti-IFC represents a mixture of antibodies to all four DAF short consensus repeat domains. The Dr(a-) individual had very low levels of Cromer-system antigens and DAF on her red cells. Loss of a TaqI restriction site from DAF exon 5 suggested that she has a previously detected mutation, encoding a Ser165Leu substitution. Red cells of the two propositi did not show abnormal levels of lysis in an acid lysis test, but after blocking of CD59 with monoclonal antibody, Inab phenotype red cells showed more lysis than Dr(a-) red cells, and Dr(a-) cells showed substantially more lysis than control cells.
Daniels
G L
GL
Bristol Institute for Transfusion Sciences, UK. geoff.daniels@msmail.nbs.nhs.uk
Green
C A
CA
Mallinson
G
G
Okubo
Y
Y
Hori
Y
Y
Kataoka
A
A
Kaihara
M
M
D000328:Adult; D001789:Blood Group Antigens / Q000235:genetics* / Q000276:immunology; D018958:CD55 Antigens / Q000235:genetics* / Q000276:immunology; D002648:Child; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D010641:Phenotype
D001789:Blood Group Antigens; D018958:CD55 Antigens