Continuous flow isoelectric focusing for purification of proteins.
To estimate the feasibility of isoelectric focusing in a continuous mode, the conditions for obtaining stable and linear pH gradients were first investigated, using a mixture of carrier ampholytes. Then the operating parameters allowing the isoelectric focusing of bovine serum albumin as model protein were determined. A theoretical approach was used to find conditions minimizing the electrohydrodynamic effect, mainly responsible for protein dispersion, for the separation of bovine serum albumin and alpha-lactalbumin from bovine milk.
Soulet
N
N
L.G.C., UMR 5503, Université Paul Sabatier, Toulouse, France. soulet@lgc.ups-tlse.fr
Roux-de Balmann
H
H
Sanchez
V
V
D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D007768:Lactalbumin / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D011506:Proteins; D012710:Serum Albumin, Bovine; D007768:Lactalbumin