Impairment of cellular redox status and membrane protein activities in kidneys from rats with ischemic acute renal failure.
Cellular redox status and membrane protein activities were analyzed in kidneys from rats with ischemic acute renal failure (ARF). ARF was induced by clamping the left renal artery for 50 min. A parallel group of control animals was processed. In the ischemic group urea plasma levels were statistically increased as compared with the control group. Studies employing whole kidney homogenates revealed that ischemia produces an increment in lipid peroxidation levels and a reduction in glutathione concentration and in superoxide dismutase and glutathione peroxidase activities. Since lipid peroxidation may alter the function of membrane proteins we determined succinate cytochrome c reductase (SuccR), sodium-potassium ATPase (Na-K-ATPase), glucose-6-phosphatase (G-6-Pase) and alkaline phosphatase (ALP) activities in whole renal homogenates. Only G-6-Pase and ALP activities were modified by ischemia. Since ALP is a brush border membrane (BBM) enzyme and BBM is one of the main target structures in ARF, we assessed some parameters of BBM functionality. ALP, gamma-glutamyl transferase (gamma-GT) and 5'-nucleotidase (5'-NT) showed diminished activities in BBM from ischemic kidneys. Ischemia also modified the Vmax of paraaminohippuric acid (PAH) uptake without altering Km. An increment of lipid peroxidation and membrane fluidity in BBM was observed after the treatment. Total membrane proteins and protein recoveries in BBM were similar in both experimental groups. Sialic acid and sulfhydryl levels were similar in BBM from ischemic kidney and control ones. In summary, ARF induced by renal artery clamping for 50 min takes place with a significant increase in urea plasma levels. A decrease in the antioxidant defense system is detected. This induces lipid peroxidation in whole renal tissue, which may justify the diminished activities of some membrane enzymes such as G-6-Pase and ALP. A specific analysis of BBM function reveals a significant increment of lipid peroxidation which may be the cause of an increased membrane fluidity. This latter parameter might be, at least in part, responsible for the damaged function of apical ALP, 5'-NT, gamma-GT and PAH carrier.
Farmacología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Suipacha, 531-2000 Rosario, Argentina.
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Montagna
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Hofer
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Torres
D058186:Acute Kidney Injury / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D005952:Glucose-6-Phosphatase / Q000032:analysis; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007511:Ischemia / Q000503:physiopathology*; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008871:Microvilli / Q000201:enzymology; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013384:Succinate Cytochrome c Oxidoreductase / Q000032:analysis; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014508:Urea / Q000097:blood; D010130:p-Aminohippuric Acid / Q000493:pharmacokinetics
D008565:Membrane Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014508:Urea; D013384:Succinate Cytochrome c Oxidoreductase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005952:Glucose-6-Phosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D005978:Glutathione; D010130:p-Aminohippuric Acid