Sialic acid, transketolase and Na+, K+, ATPase in patients with rheumatoid arthritis.
We studied transketolase activity of red blood cell hemolysates, and Na+, K+, ATPase activity and sialic acid concentration in red blood cell membranes from 52 patients with rheumatoid arthritis and 24 control subjects. Decreased red blood cell membrane Na+, K+, ATPase activity and sialic acid concentration and decreased transketolase in red blood cell hemolysates were observed in rheumatoid arthritis patients compared with control subjects (p < 0.001). Erythrocyte sedimentation rate and C-reactive protein values were increased in rheumatoid arthritis patients compared with control subjects (p < 0.0001). Significant correlations between sialic acid and Na+, K+, ATPase (r = 0.65, p < 0.001) and between sialic acid and transketolase (r = 0.58, p < 0.001) were observed. Erythrocyte sedimentation rate and C-reactive protein levels did not correlate with Na+, K+, ATPase activity or with sialic acid or transketolase in rheumatoid arthritis patients. These data show that decreases in Na+, K+, ATPase, and transketolase activities and sialic acid concentration are present in rheumatoid arthritis patients, and that the decrease in Na+, K+, ATPase and transketolase activities in rheumatoid arthritis might be due to decreased sialic acid.
Department of Biochemistry, School of Medicine, Atatürk University, Erzurum, Turkey.
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Kiziltunç
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Coğalgil
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Uğur
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Avci
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Akçay
D000328:Adult; D001172:Arthritis, Rheumatoid / Q000097:blood* / Q000201:enzymology*; D015415:Biomarkers / Q000097:blood; D001799:Blood Sedimentation; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D004910:Erythrocyte Membrane / Q000201:enzymology / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood*; D014174:Transketolase / Q000097:blood*
D015415:Biomarkers; D002097:C-Reactive Protein; D014174:Transketolase; D000254:Sodium-Potassium-Exchanging ATPase; D019158:N-Acetylneuraminic Acid