[Standardization of immunohistochemical techniques for detecting dengue virus antigens in paraffin-embedded tissues].
The immunohistochemical techniques may be very useful for the detection of antigens of the dengue virus in infected tissues. The results obtained with the use of 2 IHC methods: one direct and another indirect amplified with a biotin universal antibody and a streptavidin peroxidase system (LSAB) (the peroxidase enzyme is used in both) are shown in this paper. These methods were applied on paraffin-embedded tissues from mice that were inoculated with the dengue virus intracerebrally. Our objective is to have a technique at the laboratory allowing to identify these antigens in the tissues in order to apply them on the diagnosis and on the studies connected with the obtention of immunogens.
Instituto de Medicina Tropical Pedro Kourí.
J L
JL
Pelegrino
E
E
Arteaga
A J
AJ
Rodríguez
E
E
González
M D
MD
Frontela
M G
MG
Guzmán
D015100:3,3'-Diaminobenzidine; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis*; D019921:Biotinylation; D001921:Brain / Q000473:pathology / Q000821:virology*; D004396:Coloring Agents; D015925:Cryopreservation; D003715:Dengue / Q000473:pathology / Q000821:virology*; D003716:Dengue Virus / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000592:standards*; D051379:Mice; D016612:Paraffin Embedding
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D004396:Coloring Agents; D015100:3,3'-Diaminobenzidine