Fungal biotransformation of 6-nitrochrysene.
The fungus Cunninghamella elegans was used to biotransform 6-nitrochrysene, a mutagen that is a widespread environmental contaminant. After 6 days, 74% of the 3H-labeled 6-nitrochrysene added had been metabolized to two isomeric sulfate conjugates. These conjugates were separated by high-performance liquid chromatography and identified by UV-visible, 1H nuclear magnetic resonance, and mass spectral techniques as 6-nitrochrysene 1-sulfate and 6-nitrochrysene 2-sulfate.
Pothuluri
J V
JV
National Center for Toxicological Research, Food and Drug Administration, Jefferson, Arkansas 72079, USA. Jpothuluri@nctr.fda.gov
Sutherland
J B
JB
Freeman
J P
JP
Cerniglia
C E
CE
D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D002911:Chrysenes / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009090:Mucorales / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D002911:Chrysenes; C035986:6-nitrochrysene