Enzymatic N-riboside scission in RNA and RNA precursors.
N-ribohydrolases and transferases act on nucleosides, nucleotides and oligonucleotides to effect base removal. Advances in mechanistic and structural analysis have established that enzymes of N-riboside scission act by combinations of leaving-group and ribosyl activation. Alternative O-riboside substrates have been developed for mechanistic diagnosis. Transition-state structures have been determined, and powerful inhibitors have been designed from structural and transition-state information.
Schramm
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Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. vern@aecom.yu.edu
D000818:Animals; D016695:Glycosyltransferases / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D012263:Ribonucleosides / Q000737:chemistry / Q000378:metabolism*; D012276:Ricin / Q000378:metabolism
D012322:RNA Precursors; D012263:Ribonucleosides; D012313:RNA; D012276:Ricin; D016695:Glycosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D006867:Hydrolases