Construction of a horse BAC library and cytogenetical assignment of 20 type I and type II markers.
A horse BAC library was constructed with about 40,000 clones and mean insert size of 110 kb representing a 1.5 genome equivalent coverage and a probability of finding a single sequence of 0.75. It was characterized by PCR screening of about 130 sequences of horse microsatellites and exonic gene sequences retrieved from databases. BACs containing 8 microsatellites and 12 genes were subsequently localized by fluorescent in situ hybridization (FISH) on chromosomes. Two linkage groups were newly assigned to chromosomes: LG2 to ECA3 and LG5 to ECA24, and five linkage groups were also oriented--LG3, LG4, LG5, LG8, and LG12--leaving only three groups unassigned. This work showed how this library makes an integrated map a realistic objective for the near future and how it can make comparative mapping more efficient in a search for candidate genes of interest.
INRA, Centre de Recherche de Jouy, Laboratoire de Génétique biochimique et de Cytogénétique, 78352 Jouy-en-Josas, Cedex, France.
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Godard
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Schibler
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Oustry
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EP
Cribiu
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Guérin
D000818:Animals; D002874:Chromosome Mapping; D017931:DNA Primers; D005091:Exons; D015723:Gene Library; D005819:Genetic Markers; D006736:Horses / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction
D017931:DNA Primers; D005819:Genetic Markers