HPTLC and HPLC determination of isometamidium in the presence of its manufacturing and degradation impurities.
The determination of the phenanthridine trypanocide, isometamidium chloride hydrochloride (ISM), in the presence of four process-related and degradation impurities, by RP-HPLC using a Licrospher-60 RP-select B column with a mobile phase composition of acetonitrile/KH2PO4 (PH 3.0, 20 mM) (25:75 v/v) with UV detection at 320 nm, is described. The method is selective, reproducible and precise with a limit of detection of 45 ng ml-1 for ISM. A HPTLC system (Kieselgel 60 F254, pyridine/acetonitrile/butanol/formic acid, 6:6:4:1, v/v), with UV densitometric evaluation at 320 nm, suitable for the separation of ISM and the related substances is reported.
Department of Pharmaceutical Sciences, University of Strathclyde, Glasgow, UK.
J N
JN
Tettey
G G
GG
Skellern
J M
JM
Midgley
M H
MH
Grant
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004340:Drug Contamination; D010617:Phenanthridines / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014344:Trypanocidal Agents / Q000032:analysis* / Q000737:chemistry
D010617:Phenanthridines; D014344:Trypanocidal Agents; C000702:isometamidium chloride