Growth of Salmonella enteritidis and S. pullorum on Hektoen agar and the expression of lipopolysaccharide or flagella.
Growth of strains of Salmonella enteritidis and Salmonella pullorum on Hektoen agar has been reported to influence the expression of long-chain lipopolysaccharide and motility respectively. In this study we used a panel of strains of S. enteritidis and S. pullorum to investigate these phenomena. Culture on Hektoen agar did not cause rough strains of S. enteritidis to express long-chain lipopolysaccharide or strains of S. pullorum to become motile. It was concluded that growth of strains of S. enteritidis and S. pullorum on Hektoen agar would not normally affect the expression of somatic or flagellar antigens, and would not influence the interpretation of the Kauffman-White typing scheme.
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, UK. hcahrt@phls.co.uk
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D000362:Agar; D001434:Bacteriophage Typing; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000648:ultrastructure*; D006801:Humans; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D008854:Microscopy, Electron; D012475:Salmonella / Q000254:growth & development* / Q000378:metabolism / Q000648:ultrastructure; D012477:Salmonella enteritidis / Q000145:classification / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D008070:Lipopolysaccharides; D000362:Agar