Viruses produced from complementary DNA of virulent and avirulent strains of swine vesicular disease viruses retain the in vivo and in vitro characteristics of the parental strain.
A full-length cDNA copy of the genome of the pathogenic strain, J1'73, of swine vesicular disease virus (SVDV) was constructed and inserted into the plasmid pSVL to generate a recombinant plasmid pSVLSJ1. Infectious virus was produced following transfection of cultured mammalian cells with the plasmid. The recovered virus had the same in vitro properties as the parental strain with regard to antigenicity, plaque size on IBRS-2 cells and single-step growth. Pigs were experimentally infected with the parental virus, J1'73 strain, and viruses recovered from cells transfected with the plasmids pSVLSJ1 and pSVLS00 [Inoue T, Yamaguchi S, Saeki T, Sekiguchi K, J Gen Virol 71: 1,835-1,838 (1990)] corresponding to the pathogenic (J1'73) and non-pathogenic (H/3'76) Japanese strains of the SVDV, respectively. All pigs inoculated with the virus recovered from pSVLSJ1 produced clinical signs of similar severity to those inoculated with the parental J1'73 strain. In contrast, pigs inoculated with the virus recovered from pSVLS00 did not show any clinical signs. Viruses recovered from cells transfected with either pSVLSJ1 or pSVLS00 therefore retained the in vitro characteristics and the in vivo pathogenicity of their respective parental strains.
Department of Exotic Disease, National Institute of Animal Health, Tokyo, Japan.
T
T
Kanno
T
T
Inoue
D
D
Mackay
P
P
Kitching
S
S
Yamaguchi
J
J
Shirai
D000818:Animals; D019556:COS Cells; D018076:DNA, Complementary / Q000235:genetics*; D004770:Enterovirus / Q000235:genetics* / Q000472:pathogenicity; D013552:Swine; D013555:Swine Vesicular Disease / Q000821:virology*; D014162:Transfection; D014766:Viremia / Q000821:virology; D014774:Virulence
D018076:DNA, Complementary