Ischemia-reperfusion-induced delay in intestinal transit. Role of endothelins.
OBJECTIVE
To evaluate the involvement of ET-1 in ischemia-reperfusion (I/R) injury.


METHODS
Superior artery occlusion was performed in Wistar albino rats for 30 min followed by 2-hour (early reperfusion; ER) or 24-hour (late reperfusion; LR) reperfusion periods.


RESULTS
Intestinal transit was found to be reduced in the ER and LR groups (19.0 +/- 2.5%; p < 0.001 and 72.7 +/- 6.0%; p < 0.05, respectively) compared to the control group (85. 8 +/- 2.5%), while treatment with the ET receptor antagonist bosentan (BOS; 10 mg/kg i.v.) abolished this delay in LR. Myeloperoxidase activity showed a significant increase in ER (7.07 +/- 85.70 U/g; p < 0.001) compared to control (281.16 +/- 43.23 U/g), but BOS had no effect on this increase. The protein oxidation level was found to be higher in LR (5.92 +/- 0.77 nmol/mg protein; p < 0. 05) compared to the control (3.77 +/- 0.45 nmol/mg protein), and was reversed by BOS treatment.


CONCLUSIONS
The results of the present study imply that I/R delays intestinal transit involving an endothelin-dependent mechanism.
Department of Physiology, Marmara University, School of Medicine, Haydarpaşa, Istanbul, Turkey.
I
I
Alican
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C
Yegen
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A
Olcay
H
H
Kurtel
B
B
Yegen
D000818:Animals; D000959:Antihypertensive Agents / Q000627:therapeutic use; D000077300:Bosentan; D004195:Disease Models, Animal; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D005769:Gastrointestinal Motility / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000098:blood supply / Q000503:physiopathology*; D015227:Lipid Peroxidation; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy / Q000378:metabolism / Q000503:physiopathology; D013449:Sulfonamides / Q000627:therapeutic use
D000959:Antihypertensive Agents; D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D013449:Sulfonamides; D009195:Peroxidase; D005978:Glutathione; D000077300:Bosentan