Genetic variation of the Borrelia burgdorferi gene vlsE involves cassette-specific, segmental gene conversion.
The Lyme disease spirochete Borrelia burgdorferi possesses 15 silent vls cassettes and a vls expression site (vlsE) encoding a surface-exposed lipoprotein. Segments of the silent vls cassettes have been shown to recombine with the vlsE cassette region in the mammalian host, resulting in combinatorial antigenic variation. Despite promiscuous recombination within the vlsE cassette region, the 5' and 3' coding sequences of vlsE that flank the cassette region are not subject to sequence variation during these recombination events. The segments of the silent vls cassettes recombine in the vlsE cassette region through a unidirectional process such that the sequence and organization of the silent vls loci are not affected. As a result of recombination, the previously expressed segments are replaced by incoming segments and apparently degraded. These results provide evidence for a gene conversion mechanism in VlsE antigenic variation.
Department of Pathology and Laboratory Medicine and Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, Texas 77030, USA.
J R
JR
Zhang
S J
SJ
Norris
D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics*; D000954:Antigens, Surface / Q000235:genetics*; D001426:Bacterial Proteins; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial; D004195:Disease Models, Animal; D005785:Gene Conversion; D005798:Genes, Bacterial; D014644:Genetic Variation; D008074:Lipoproteins / Q000235:genetics*; D008193:Lyme Disease / Q000382:microbiology*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D005810:Multigene Family; D012689:Sequence Homology, Nucleic Acid
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008074:Lipoproteins; C105546:VlsE protein, Borrelia burgdorferi