Retinoid X receptor-gamma gene expression is developmentally regulated in the embryonic rodent peripheral nervous system.
The retinoid X receptor-gamma (RXRgamma) is a transcription factor that belongs to the thyroid hormone/retinoid family of nuclear receptors. Previous studies have shown that RXRgamma is expressed in the developing peripheral nervous system (PNS) of chick embryos, but such expression has not been reported previously in rodent embryos. Indeed, the pattern of RXRgamma expression appears to be different between avian and rodent species. Using in situ hybridisation and northern blot experiments we show that RXRgamma is expressed in elements of the PNS in rat and mouse embryos and in postnatal rats. However, unlike the chick, where RXRgamma is expressed from the onset of neural crest migration, rat RXRgamma expression in the dorsal root ganglia and trigeminal ganglia was detectable at 14.5 days post coitum (dpc), but not at 13, 12, or 11 dpc. These data suggest that RXRgamma may have a role in the dorsal root ganglia and trigeminal ganglia of late embryos, and that this could be evolutionarily conserved between chick and rodent. On the other hand, rat RXRgamma transcripts in the facio-acoustic (VII-VIII) ganglion were detectable at 11 dpc in neural crest cells condensing to form this ganglion. We discuss the possible significance of the timing of RXRgamma expression in the developing PNS and suggest that closer examination of the structure and function of the PNS of RXRgamma null mutant mice would be of interest.
Georgiades
P
P
Department of Molecular Pathology, University College London Medical School, UK. pg3@mole.bio.cam.ac.uk
Wood
J
J
Brickell
P M
PM
D000818:Animals; D015152:Blotting, Northern; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000196:embryology* / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D018168:Receptors, Retinoic Acid / Q000235:genetics / Q000378:metabolism*; D047488:Retinoid X Receptors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D009687:Nuclear Proteins; D018168:Receptors, Retinoic Acid; D047488:Retinoid X Receptors; D014157:Transcription Factors