Age- and temperature-dependent effects of opioids on medulla oblongata respiratory activity: an in vitro study in newborn rat.
Influences of post-natal age (P0-P4) and temperature (22.5 degrees-31.5 degrees C) on the action(s) of opioids on respiratory activities from neonatal rat brainstem-spinal cord preparations were examined in this study. A temperature-dependent mu-opioid receptor effect on respiration was found. In addition, the effect of morphine increased with postnatal age (P0-P4). Hence, age and temperature must be taken into account when performing studies on medullary respiration-related structures using the neonatal rat brainstem-spinal cord preparation.
Department of Anesthesiology and Intensive Care, Hokkaido University School of Medicine, Kita-15, Nishi-7, Kita-ku, Sapporo 060, Japan.
K
K
Takita
E
E
Herlenius
S G
SG
Lindahl
Y
Y
Yamamoto
D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer / Q000494:pharmacology; D000367:Age Factors; D000700:Analgesics / Q000494:pharmacology; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology; D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D020881:Enkephalin, D-Penicillamine (2,5)-; D004745:Enkephalins / Q000494:pharmacology; D066298:In Vitro Techniques; D008526:Medulla Oblongata / Q000187:drug effects* / Q000502:physiology*; D009020:Morphine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012119:Respiration / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology; D013696:Temperature
D000700:Analgesics; D018712:Analgesics, Non-Narcotic; D000701:Analgesics, Opioid; D004745:Enkephalins; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D019900:3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer; D009020:Morphine; D020881:Enkephalin, D-Penicillamine (2,5)-