Single molecule biochemistry using optical tweezers.
The use of optical trapping to create extremely compliant mechanical probes has ushered in a new field of biological inquiry, the mechanical and kinetic study of proteins at the single molecule level. This review focuses on three examples of such study and includes methods of extracting parameters of interest from the raw data such experiments generate.
Mehta
A D
AD
Department of Biochemistry, Stanford University Medical Center, CA 94305, USA.
Pullen
K A
KA
Spudich
J A
JA
D001671:Biochemistry / Q000295:instrumentation*; D007834:Lasers; D055095:Optics and Photonics; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000032:analysis*
D011506:Proteins