Differential localization of GABA(A) receptor alpha and beta subunits in the hamster retina and relationship with glutamic acid decarboxylase immunoreactivity.
In order to determine the cellular localization of GABA(A)alpha and beta subunits in the hamster retina, single and double immunocytochemistry was performed in perfuse-fixed hamster retina using commercially-available antibodies against the two receptor subunits and glutamic acid decarboxylase. Strong GABA(A)beta immunoreactivity was found in two strata of the inner plexiform layer and in perikarya of amacrine cells and bipolar cells in the inner nuclear layer. In contrast, no GABA(A)alpha immunoreactivity was detected. All but a few of the GABA(A)beta-immunoreactive amacrine cells were found not to display glutamic acid decarboxylase immunoreactivity. The present results indicate that there is a differential localization of GABA(A)alpha and beta subunits in different neuronal subpopulations in the hamster retina.
Department of Biology, Hong Kong Baptist University, Kowloon Tong, China.
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Lee
E Y
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Cho
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Yung
D000818:Animals; D015415:Biomarkers; D006224:Cricetinae; D005260:Female; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000276:immunology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008647:Mesocricetus; D018377:Neurotransmitter Agents / Q000378:metabolism; D011963:Receptors, GABA-A / Q000378:metabolism* / Q000502:physiology; D012160:Retina / Q000378:metabolism*
D015415:Biomarkers; D018377:Neurotransmitter Agents; D011963:Receptors, GABA-A; D005968:Glutamate Decarboxylase