A novel oestrogen-regulated gene in human breast cancer cells identified by differential display.
Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859 of the published sequence for the mRNA for human megakaryocyte CD63 antigen but has a 3' tail extended by 23 nucleotides. Northern blotting has confirmed that this mRNA is regulated by oestrogen in both MCF7 and T47D human breast cancer cell lines.
Stephen
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Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, The University of Reading, Whiteknights, UK.
Corcoran
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Darbre
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D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060149:Tetraspanin 30; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; C554586:CD63 protein, human; D018076:DNA, Complementary; D004967:Estrogens; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D060149:Tetraspanin 30