Influence of 5-lipoxygenase on in vitro growth of human mammary carcinoma cell line MCF-7.
The aim of this study was to investigate the direct effect of 5-lipoxygenase (5-LO) on the growth of human mammary cancer cells MCF-7 in vitro. Cell growth was measured according to the level of 3H-thymidine incorporation. 5-LO was shown to inhibit 3H-thymidine incorporation. The inhibitory effect was 19, 42 and 78% when administered at concentrations of 0.1, 0.2 or 0.5 U/ml, respectively. Its effect was time- and dose-dependent and was statistically significant at concentrations of 0.2 and 0.5 U/ml. We have also shown that the specific 5-LO inhibitor MK-886 (1 microM) lifts the inhibitory effect of 5-LO (0.2 U/ml). Moreover, when treated with an activator of 5-lipoxygenase calcium ionophore A23187 (10 microM) MCF-7 cells synthesize LTB4. The results of this study are evidence of the role of 5-lipoxygenase in the regulation of human mammary cancer cells growth in vitro.
Przylipiak
A
A
Department of Obstetrics and Gynecology, University of Heidelberg, Germany.
Hafner
J
J
Przylipiak
J
J
Köhn
F M
FM
Runnebaum
B
B
Rabe
T
T
D001094:Arachidonate 5-Lipoxygenase / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology*; D000001:Calcimycin / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007211:Indoles / Q000494:pharmacology; D007975:Leukotriene B4 / Q000096:biosynthesis; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014407:Tumor Cells, Cultured; D002722:Chlorobenzenes
D001094:Arachidonate 5-Lipoxygenase; D000001:Calcimycin; D007211:Indoles; D007975:Leukotriene B4; D016859:Lipoxygenase Inhibitors; D013936:Thymidine; C060893:MK-886; D002722:Chlorobenzenes