Novel p53 tumour suppressor mutations in cases of spindle cell sarcoma, pleomorphic sarcoma and fibrosarcoma in cats.
Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG-->GAG, lysine-->glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
B
B
Mayr
M
M
Reifinger
K
K
Alton
G
G
Schaffner
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000235:genetics* / Q000473:pathology; D002415:Cats; D004273:DNA, Neoplasm / Q000737:chemistry; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D017353:Gene Deletion; D016158:Genes, p53 / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012509:Sarcoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*
D004273:DNA, Neoplasm