Cloning and characterisation of the Proteus mirabilis xerD gene.
The Xer site-specific recombination system is involved in the stable maintenance of replicons (certain plasmids and chromosomes) in Escherichia coli and other bacteria by converting multimers into monomers. This system requires a cis-acting DNA sequence (the chromosomal dif site or the ColE1 cer site) and two trans-acting factors: the XerC and XerD recombinases, which belong to the lambda integrase family of tyrosine site-specific recombinases. In addition, in order to resolve plasmid multimers into monomers, two additional factors are required: the ArgR and PepA proteins. We have previously shown the presence of xerC and xerD genes (and their function) by Southern hybridisation and by in vivo recombination in a wide variety of Enterobacteriaceac. We have now cloned and sequenced the xerD gene of Proteus mirabilis using degenerate and inverse PCR methods. This gene encodes a tyrosine recombinase which is highly similar to the E. coli XerD recombinase, is capable of complementing an E. coli xerD mutant, and displays sequence-specific DNA binding activity.
Dèpartement de Microbiologie et Immunologie, Université de Montréal, Que., Canada.
M
M
Villion
G
G
Szatmari
D000595:Amino Acid Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D004254:DNA Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D019426:Integrases; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011513:Proteus mirabilis / Q000737:chemistry / Q000235:genetics*; D045522:Recombinases; D016415:Sequence Alignment
D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; D045522:Recombinases; C473360:XerC protein, E coli; D004254:DNA Nucleotidyltransferases; D019426:Integrases; C473361:XerD protein, E coli; C115006:integron integrase IntI1