The embryogenesis of rod photoreceptors in the teleost fish retina, Haplochromis burtoni.
Development of the retina, like that of other tissues, occurs via an orderly sequence of cell division and differentiation, producing the functional retina. In teleost fish, however, cell division and differentiation in the retina continue throughout the life of the animal in two distinct ways. Stem cells in a circumferential germinal zone at the periphery of the retina give rise to all retinal cell types and progenitor cells located throughout the retina in the outer nuclear layer (ONL) produce new rod photoreceptors. These processes in adult retina recapitulate in space the embryonic events responsible for forming the retina. Analysis of these events in an African cichlid fish, Haplochromis burtoni, confirmed that cone photoreceptors differentiate first, followed by rod photoreceptors. Correspondingly, at the margin of the eye, cone photoreceptors differentiate nearer to the margin than do rods. Control of photoreceptor production is not understood. Here we present the time of appearance and distribution pattern of GABA and vimentin which are candidates for the control of retinal cell division and differentiation. Antibody staining reveals that both GABA and vimentin exhibit unique patterns of expression during embryonic retinal development. Vimentin immunoreactivity is evident throughout the retina in a spoke-like pattern between developmental Days 4 and 7, as both cone and rod photoreceptors are being formed. GABA is expressed in horizontal cells between Days 5 and 7, corresponding to the onset of rod differentiation in time and in position within the retina. Moreover, the wave of GABAergic staining in the horizontal cells parallels the wave of rod differentiation across the embryonic retina of H. burtoni. Thus, GABA may play a role in the development of rod photoreceptors.
Institute of Neuroscience, University of Oregon, Eugene 97403, USA. mhagedorn@nzp.si.edu
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M
Hagedorn
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Mack
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Evans
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Fernald
D000818:Animals; D005260:Female; D008297:Male; D010471:Perches / Q000196:embryology*; D017949:Retinal Cone Photoreceptor Cells / Q000737:chemistry / Q000196:embryology; D017948:Retinal Rod Photoreceptor Cells / Q000737:chemistry / Q000196:embryology*; D014746:Vimentin / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis
D014746:Vimentin; D005680:gamma-Aminobutyric Acid