Changes in GluR4 expression induced by metabotropic receptor activation in radial glia cultures.
The expression of neurotransmitter receptors in glial cells has suggested a regulatory role of these cells in synaptic function. In radial glia, glutamate receptors elicit a cascade from the membrane to the nucleus and a consequent change in gene expression. In order to gain insight into this process, we address the question of whether receptor activation leads to changes in the repertoire of AMPA/KA glutamate receptor subunits in Bergmann and Müller glial cells. Of the subunits investigated, only GluR4 was up-regulated in Bergmann glial cells both at mRNA and protein levels. In contrast, in Müller glial cells Glu treatment leads to a reduction in GluR4 mRNA and protein expression. Both effects are receptor-mediated and must probably involve group I of metabotropic glutamate receptors. Accordingly, using Northern blot analysis and RT-PCR we detected the expression of both mGluR1 and mGluR5 transcripts in the cultured cells. Our results confirm that glutamate receptors in Bergmann and Müller cells modulate gene expression and further strengthen a plausible role of glial cells in long-lasting changes in the central nervous system.
López
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Departamento de Genética y Biología Molecular, CINVESTAV-IPN, Apartado Postal 14-740, México D.F. 07000, Mexico.
López-Colomé
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AM
Ortega
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D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000196:embryology / Q000378:metabolism; D002642:Chick Embryo; D003515:Cycloleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology*; D007608:Kainic Acid / Q000494:pharmacology; D007700:Kinetics; D016202:N-Methylaspartate / Q000494:pharmacology; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D064529:Receptor, Metabotropic Glutamate 5; D018091:Receptors, AMPA / Q000096:biosynthesis*; D018094:Receptors, Metabotropic Glutamate / Q000096:biosynthesis*; D012160:Retina / Q000166:cytology / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
D003515:Cycloleucine; D018698:Glutamic Acid; D007608:Kainic Acid; D016202:N-Methylaspartate; D012333:RNA, Messenger; D064529:Receptor, Metabotropic Glutamate 5; D018091:Receptors, AMPA; D018094:Receptors, Metabotropic Glutamate; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; C104724:glutamate receptor ionotropic, AMPA 4; C104077:metabotropic glutamate receptor type 1; C036361:1-amino-1,3-dicarboxycyclopentane