Rapid fluorescence-based measurement of neutrophil migration in vitro.
We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.
Medical Research Service of the Seattle VA Medical Center, Department of Medicine, University of Washington School of Medicine, 98108, USA. cfrevert@u.washington.edu
C W
CW
Frevert
V A
VA
Wong
R B
RB
Goodman
R
R
Goodwin
T R
TR
Martin
D002452:Cell Count; D002634:Chemotaxis, Leukocyte; D005069:Evaluation Studies as Topic; D005452:Fluoresceins; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D008851:Micropore Filters; D009504:Neutrophils / Q000502:physiology*; D011076:Polycarboxylate Cement; D011091:Polyesters; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D005452:Fluoresceins; D005456:Fluorescent Dyes; D011076:Polycarboxylate Cement; D011091:Polyesters; C005506:polycarbonate; C007740:fluorexon