Synthesis of terminal disaccharide elements corresponding to the Ogawa and Inaba antigenic determinant from Vibrio cholerae O1.
Vibrio cholerae O1 LPS terminal mono- and disaccharide elements were synthesized by reduction of the azido group in several 4-amino-4,6-dideoxy-D-mannose mono- and disaccharide derivatives, followed by coupling with 2, 4-di-O-acetyl-3-deoxy-L-glycero-tetronic acid in the presence of 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline. This compound represents a useful model in order to elucidate the size of the epitopes which define Ogawa and Inaba serotypes from Vibrio cholerae O1.
Arencibia-Mohar
A
A
Laboratory of Synthetic Antigens, Facultad de Química, Universidad de La Habana, Cuba.
Ariosa-Alvarez
A
A
Madrazo-Alonso
O
O
González Abreu
E
E
García-Imia
L
L
Sierra-González
G
G
Verez-Bencomo
V
V
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000276:immunology; D002236:Carbohydrate Conformation; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry*; D006885:Hydroxybutyrates / Q000138:chemical synthesis* / Q000737:chemistry; D008070:Lipopolysaccharides / Q000737:chemistry*; D051379:Mice; D009005:Monosaccharides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D009904:Optical Rotation; D012703:Serotyping; D014734:Vibrio cholerae / Q000145:classification / Q000276:immunology*
C114469:2,4-di-O-acetyl-3-deoxy-glycero-tetronic acid; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004187:Disaccharides; D000939:Epitopes; D006885:Hydroxybutyrates; D008070:Lipopolysaccharides; D009005:Monosaccharides