Intermolecular beta-sheet results from trifluoroethanol-induced nonnative alpha-helical structure in beta-sheet predominant proteins: infrared and circular dichroism spectroscopic study.
2,2,2-Trifluoroethanol (TFE)-induced nonnative alpha-helical structure in peptides and proteins has been extensively studied with circular dichroism (CD) spectroscopy. However, to date, complementary information from infrared (IR) spectroscopy has not been reported. Using both IR and CD spectroscopy, we demonstrate here that the TFE-induced nonnative alpha-helical structure in two beta-sheet-predominant proteins, beta-lactoglobulin and alpha-chymotrypsin, is unstable in comparison with those found in the alpha-helix-predominant proteins myoglobin and cytochrome c under identical conditions. IR spectra showed that, immediately after dissolution of the beta-sheet proteins in 50% (v/v) TFE, a strong amide I band component appears at 1654 cm-1 in H2O and at 1650 cm-1 in D2O, which is ascribed to alpha-helical structure. However, the intensities of the alpha-helical bands decrease as a function of time, concomitant with the appearance of two new band components near 1620 and 1695 cm-1 in H2O and 1612 and 1684 cm-1 in D2O, a typical IR spectral pattern for an intermolecular beta-sheet aggregate. Clear gels begin to develop within 30 min. No similar spectral changes were observed for the alpha-helical proteins. CD spectra suggested initially that the TFE-induced alpha-helix was retained in the gelled state. However, further analysis of the spectra, and Gaussian function modeling with basic spectra, indicated that the apparent alpha-helix signal was actually due to a combination of signals from intermolecular beta-sheet and residual alpha-helix. These results indicate that the TFE-induced nonnative alpha-helix structure in predominantly beta-sheet proteins is unstable and readily converts to an intermolecular beta-sheet aggregate.
Department of Chemistry and Biochemistry, University of Northern Colorado, Greeley, Colorado, 80639, USA.
A
A
Dong
J
J
Matsuura
M C
MC
Manning
J F
JF
Carpenter
D000818:Animals; D002417:Cattle; D002918:Chymotrypsin / Q000737:chemistry / Q000187:drug effects; D002942:Circular Dichroism; D003574:Cytochrome c Group / Q000737:chemistry / Q000187:drug effects; D007782:Lactoglobulins / Q000737:chemistry / Q000187:drug effects; D009211:Myoglobin / Q000737:chemistry / Q000187:drug effects; D017433:Protein Structure, Secondary / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D014270:Trifluoroethanol / Q000494:pharmacology*
D003574:Cytochrome c Group; D007782:Lactoglobulins; D009211:Myoglobin; D014270:Trifluoroethanol; D002918:Chymotrypsin