A novel repetitive sequence of sugar cane, SCEN family, locating on centromeric regions.
Tandem repetitive sequences consisting of 140-bp repetitive units were cloned from sugar cane genomic DNA and designated the SCEN family. In situ hybridization revealed that they were located on the centromeric region of almost all of the chromosomes of sugar cane. The 140-bp sequence included three CENP-B box-like sequences. Phylogenetic analysis of the members of the SCEN family revealed that the sequences had 75% homology with each other, on average, and that the sequences could not be further classified into smaller subfamilies. The copy number of the sequence was estimated to be 2.6 x 10(5) per haploid sugar cane genome and, therefore, 4.6 x 10(3) or 630 kb per chromosome on average.
Kihara Institute Biological Research, Yokohama City University, Yokohama, Japan. nagaki@yokohama-cu.ac.jp
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Nagaki
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Tsujimoto
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Sasakuma
D001324:Autoantigens; D015139:Blotting, Southern; D002503:Centromere / Q000235:genetics*; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018744:DNA, Plant / Q000235:genetics*; D004268:DNA-Binding Proteins; D014644:Genetic Variation; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D005810:Multigene Family; D010802:Phylogeny; D018515:Plant Leaves; D018517:Plant Roots; D010944:Plants / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA
D001324:Autoantigens; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D018744:DNA, Plant; D004268:DNA-Binding Proteins