Quantitative recovery of Man9GlcNAc2Asn derivatives from concanavalin A.
Some larger high-mannose-type oligosaccharides bind very tightly to concanavalin A and are difficult to elute. We present conditions that permit the complete elution of compounds containing high-mannose type oligosaccharides from a popular concanavalin A-Sepharose that is commercially available. Europium-labeled Man9GlcNAc2Asn (N. Kawasaki and Y.C. Lee, Anal. Biochem., 250 (1997) 260-262), soybean agglutinin, and Eu(III)-labeled soybean agglutinin bound to concanavalin A-Sepharose were completely eluted with 1 M methyl alpha-D-mannopyranoside by allowing the column to stand in elution buffer, permitting the oligosaccharide or glycoprotein to slowly dissociate from the column.
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Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
Kawasaki
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Lee
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D001216:Asparagine / Q000737:chemistry; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D005063:Europium / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*
D006020:Glycopeptides; D009844:Oligosaccharides; D003208:Concanavalin A; D005063:Europium; D001216:Asparagine; D008358:Mannose