Effects of vascular permeability factor on the permeability of cultured endothelial cells from brain capillaries.
The effects of vascular permeability factor (VPF) on the blood-brain barrier (BBB) permeability were studied in cultured capillary endothelial cells from bovine brain. When VPF was added to the abluminal or brain side of the BBB, a rapid increase in the BBB permeability was observed. This increase in the BBB permeability is dependent on the concentration of the VPF used and is reversible on removal of the VPF. Contrarily, when VPF was added to the luminal or blood side of the BBB, no increase in the BBB permeability was observed. These results suggest that the receptors for VPF are located on the abluminal side of the BBB.
Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, Denver, USA.
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D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects; D002196:Capillaries / Q000166:cytology / Q000187:drug effects; D002199:Capillary Permeability / Q000187:drug effects*; D002417:Cattle; D002478:Cells, Cultured; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005452:Fluoresceins; D005456:Fluorescent Dyes; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008222:Lymphokines; D012333:RNA, Messenger; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; C024098:6-carboxyfluorescein