Conformational and dynamic properties of a 14 residue antifreeze glycopeptide from Antarctic cod.
The 1H and 13C NMR spectra of a 14-residue antifreeze glycopeptide from Antarctic cod (Tetramatomnus borchgrevinki) containing two proline residues have been assigned. 13C NMR relaxation experiments indicate motional anisotropy of the peptide, with a tumbling time in water at 5 degrees C of 3-4 ns. The relaxation data and lack of long-range NOEs are consistent with a linear peptide undergoing significant segmental motion. However, extreme values of some coupling constants and strong sequential NOEs indicate regions of local order, which are most evident at the two ATPA subsequences. Similar spectroscopic properties were observed in the 16-residue analogue containing an Arg-Ala dipeptide added to the C-terminus. Molecular modeling also showed no evidence of long-range order, but the two ATPA subsequences were relatively well determined by the experimental data. These motifs were quite distinct from helical structures or beta turns commonly found in proteins, but rather resemble sections of an extended polyproline helix. Thus, the NMR data provide a description of the local order, which is of relevance to the mechanism of action of the antifreeze activity of the antifreeze glycopeptides as well as their ability to protect cells during hypothermic storage.
Division of Molecular Structure, National Institute for Medical Research, Mill Hill, London, United Kingdom. alane@nimr.mrc.ac.uk
A N
AN
Lane
L M
LM
Hays
R E
RE
Feeney
L M
LM
Crowe
J H
JH
Crowe
D000595:Amino Acid Sequence; D000818:Animals; D000864:Antarctic Regions; D021301:Antifreeze Proteins; D015925:Cryopreservation; D005399:Fishes; D005615:Freezing; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D006860:Hydrogen Bonding; D007053:Ice; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011392:Proline; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary
D021301:Antifreeze Proteins; D006023:Glycoproteins; D007053:Ice; D011392:Proline