Thin-layer chromatographic determination of monensin in feeds: screening method.
Monensin is extracted from feed with methanol and purified by solvent-partitioning solid-phase extraction. After solvent reduction, monensin is separated by thin-layer chromatography on silica gel and visualized by color development with vanillin. No false-positive results were obtained in validation studies by submitting or peer laboratories when blank samples were analyzed. Three of 20 samples spiked with 5 ppm monensin were reported as containing no monensin. All samples spiked with 10 ppm monensin were reported positive for monensin.
Landgraf
W W
WW
U. S. Department of Agriculture, National Veterinary Services Laboratories, Ames, IA 50010, USA.
Ross
P F
PF
D000821:Animal Feed / Q000032:analysis*; D000935:Antifungal Agents / Q000032:analysis*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D008985:Monensin / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results
D000935:Antifungal Agents; D007202:Indicators and Reagents; D008985:Monensin