[Influence of infusion solution on the vascular permeability in rat skin].
In this study, we attempted to confirm the assessment system of incidence of angialgia and thrombophlebitis by evaluating the influence of test solutions on the vascular permeability by intradermal injection into rat skin, and following results were obtained: 1) Dimensions of dye leakage in the rat skin were not increased by injection of one commercially available preparation (solution 1), but increased significantly by injection of a preparation (solution 2) that had induced a high incidence of angialgia in a clinical study. 2) Dimensions of the dye leakage increased significantly by injection of glucose solutions with about four degrees of osmolality ratio. 3) In the injection of acetate buffers with different titratable acidity, dimensions of the dye leakage increased depending on titratable acidity. 4) Solution 1 was adjusted to pH 4.43 with L-lactate, acetic acid of HCl, and then these solutions were intradermally injected to rats. The influence on dimensions of the dye leakage was in the following order of strength: acetic acid >> L-lactate > HCl. These results suggest that the vascular permeability by injection into rat skin is influenced by osmolality, pH, titratable acidity and composition of test solutions. Therefore, this system using the vascular permeability reaction in rat skin may be useful for evaluation of angialgia and thrombophlebitis incidence.
Kawasaki
M
M
Infusion Research Department, Hoechst Marion Roussel Ltd., Shiga, Japan.
Kimura
I
I
Matsuda
A
A
Kataoka
M
M
Kokuba
Y
Y
D019342:Acetic Acid / Q000494:pharmacology; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects*; D004396:Coloring Agents; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007263:Infusions, Parenteral; D007271:Injections, Intradermal; D007773:Lactates / Q000494:pharmacology; D008297:Male; D009997:Osmotic Pressure; D010146:Pain / Q000209:etiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000098:blood supply*; D013924:Thrombophlebitis / Q000209:etiology; D014652:Vascular Diseases / Q000209:etiology
D004396:Coloring Agents; D007773:Lactates; D019342:Acetic Acid; D006851:Hydrochloric Acid