Interaction of p27 with E1A and its effect on CDK kinase activity.
The interaction of p27 with adenovirus (Ad) E1A was investigated to study its possible role in cell-cycle regulation and transformation by E1A. In in vitro binding assays, recombinant p27 proteins were shown to bind 12S and 13S E1A products of both Ad12 and Ad5. The amino-terminal region of p27, but not the carboxyl-terminal region, was responsible for the E1A binding. In the Ad12 E1A proteins, the C-terminal region showed relative importance in p27 binding. Phosphorylation of histone H1 or E1A proteins by CDK2 complex was inhibited by p27, but, in contrast, p27 stimulated the phosphorylation of E1A proteins by CDK4. Thus, the interaction of p27 and E1A proteins may modulate the function of E1A in cell-cycle control by regulating E1A phosphorylation.
Nomura
H
H
Department of Laboratory Medicine, Miyazaki Medical College, Kiyotake, 889-1692, Japan.
Sawada
Y
Y
Ohtaki
S
S
D000256:Adenoviridae / Q000737:chemistry*; D017500:Adenovirus E1A Proteins / Q000737:chemistry*; D000818:Animals; D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins; D002460:Cell Line; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D050760:Cyclin-Dependent Kinase Inhibitor p27; D018844:Cyclin-Dependent Kinases / Q000378:metabolism / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000494:pharmacology*; D010766:Phosphorylation; D011485:Protein Binding / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D011506:Proteins; D011518:Proto-Oncogene Proteins; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins / Q000378:metabolism; D000095043:Cyclin-Dependent Kinase-Activating Kinase
D017500:Adenovirus E1A Proteins; C493962:Cdkn1b protein, rat; D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D014764:Viral Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495892:Cdk2 protein, rat; C495903:Cdk4 protein, rat; D051357:Cyclin-Dependent Kinase 2; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase