Analysis of two transcription activation elements in the promoter of the developmentally regulated con-10 gene of Neurospora crassa.
The con-10 gene of Neurospora crassa is activated during conidiation. CRS-B (conidiation response sequence-B) and CGE (con-10 general enhancer) elements have been proposed to function as sites of transcriptional activation and as possible elements that confer developmental regulation to con-10. In a specific analysis of the roles of these elements we found that two CRS-B elements are necessary for full activation of con-10 during macroconidiation, whereas two CGE elements are functionally redundant, such that a single CGE is sufficient for maximal expression. However, CRS-B and CGE elements are not sufficient for developmental activation of a reporter gene. The CRS-B element was further dissected and one of the CRS-B elements appears to function in repression as well as activation. con-10 is also highly expressed during microconidiation, a different form of asexual sporulation, but we show here that CRS-B elements do not play a significant role in con-10 expression during microconidiation. Both CRS-B elements contribute to basal con-10 expression during mycelial growth. con-10 is also regulated by light, and CRS-B and CGE elements may play minor roles in controlling con-10 expression in response to light.
Lee
K
K
Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843-2132, USA.
Ebbole
D J
DJ
D001483:Base Sequence; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D008027:Light; D009154:Mutation; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D017384:Sequence Deletion; D015533:Transcriptional Activation; D001616:beta-Galactosidase / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase