Gradual solubilization of Candida cell wall beta-glucan by oxidative degradation in mice.
Candida spp. is a medically important fungi which induces disseminated candidiasis and candidemia in hospitalized immunocompromised patients. The cell wall of Candida is mainly composed of two polysaccharides, mannan and beta-glucan, and at least part of beta-glucan is basically insoluble in H2O or NaOH. We became interested in when and how particulate beta-glucan changes to the soluble form. However, the fate of wall components has not been examined in detail. In this study, modification and solubilization of the cell wall beta-glucan were analyzed in vivo and in vitro. Cells of Candida, intravenously administered to mice (1 mg/mouse), were immediately deposited mainly in liver as determined by 3H-labeled cells. Beta-Glucans were detected in these mice for at least for 6 months by the beta-glucan specific assay. During this period, the insoluble cell wall beta-glucan was gradually solubilized in these organs, probably by oxidative stress of macrophages. Candida cells and particulate beta-glucans were also gradually solubilized in vitro using sodium hypochlorite solution, but part of the cell wall beta-glucan was still insoluble even after treatment with concentrated hypochlorite solution for one day at room temperature. These findings strongly suggested that the fungal cell wall beta-glucans were quite resistant to oxidative metabolism in vivo and in vitro, and thus deposited for quite long period in the host.
Miura
N N
NN
Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
Miura
T
T
Ohno
N
N
Adachi
Y
Y
Watanabe
M
M
Tamura
H
H
Tanaka
S
S
Yadomae
T
T
D000818:Animals; D002175:Candida / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D002177:Candidiasis / Q000378:metabolism / Q000382:microbiology*; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D005936:Glucans / Q000737:chemistry* / Q000378:metabolism; D008033:Limulus Test; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction; D012972:Sodium Hydroxide / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D047071:beta-Glucans
D005936:Glucans; D047071:beta-Glucans; D012972:Sodium Hydroxide; C033363:beta-1,3-glucan; D012973:Sodium Hypochlorite