Analysis of conversion tracts associated with recombination events at the am locus of Neurospora crassa.
In cross B163, heteroallelic am1 am6 and heterozygous for both conventional genetic flanking markers and closer molecular markers, we previously found that the majority on flanker exchanges were remote from events that generated prototrophic recombinants. We report here that natural polymorphisms distinguishing the parents of cross B163 also include sequences within and closely flanking am. Segregation of these markers in B163 prototrophs confirms that the majority of meiotic recombination events at am resulted from gene conversion. Conversion of am6, the distal allele, is more frequent than conversion of am1. Twelve percent of tracts in am6 convertants were discontinuous while 30% of continuous tracts converting am6 extend less than 741 bp.
School of Biological Sciences, Flinders University of South Australia, Bedford Park, South Australia 5042, Australia.
F J
FJ
Bowring
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DE
Catcheside
D000483:Alleles; D001483:Base Sequence; D003434:Crossing Over, Genetic; D005091:Exons; D005785:Gene Conversion; D005800:Genes, Fungal; D005819:Genetic Markers; D007438:Introns; D009492:Neurospora crassa / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017421:Sequence Analysis
D005819:Genetic Markers