Localisation of the imprinted gene neuronatin, Nnat, confirms and refines the location of a second imprinting region on mouse chromosome 2.
Nine regions on six mouse autosomes are subject to imprinting and uniparental inheritance of any one of these regions results in mice with phenotypic anomalies. So far on distal Chromosome (Chr) 2 there is a unique imprinting region between 2H3 and 2H4 associated with two behavioural disorders and neonatal lethality. A maternally imprinted gene, Nnat, has been identified which is expressed in the nervous system and maps to distal Chr 2. Nnat has been excluded as a candidate for either or both the behavioural phenotypes as it lies proximal to the 2H3-2H4 imprinting region. Here we have mapped Nnat to band 2H1 which is at least 18 Mb proximal to the previously described imprinting region. It maps close to agouti, some alleles of which show differential expression according to parental origin. The localisation of Nnat to band H1 confirms and refines the map location of a second imprinting region on mouse Chr 2.
Mammalian Genetics Unit, Medical Research Council, Harwell, Didcot, Oxfordshire (UK). c.williamson@har.mrc.ac.uk
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Williamson
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Beechey
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Dutton
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Cattanach
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Ishino
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Peters
D000818:Animals; D019485:Bone Morphogenetic Proteins; D002873:Chromosome Fragility; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D005260:Female; D005796:Genes / Q000235:genetics*; D018392:Genomic Imprinting / Q000235:genetics*; D055428:Growth Differentiation Factor 5; D006133:Growth Substances / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009115:Muridae; D009419:Nerve Tissue Proteins / Q000235:genetics*; D051966:Phospholipase C gamma; D014178:Translocation, Genetic; D010738:Type C Phospholipases / Q000235:genetics
D019485:Bone Morphogenetic Proteins; C525894:Gdf5 protein, mouse; D055428:Growth Differentiation Factor 5; D006133:Growth Substances; D007527:Isoenzymes; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse; D010738:Type C Phospholipases; D051966:Phospholipase C gamma