What's in that band?
The analysis of molecular variation in parasites has important implications for studying gene function and organisation, taxonomy, phylogeny and population genetics. Polymerase chain reaction-based mutation scanning methods can have significant advantages over some currently used DNA approaches for the analysis of allelic and mutational sequence variation in parasites. The present report describes briefly the principles of some of these methods, examines some of their advantages and disadvantages, and indicates their potential for applications in parasitology.
Department of Veterinary Science, University of Melbourne, Werribee, Victoria, Australia. gasser@ariel.ucs.unimelb.edu.au
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Gasser
D000818:Animals; D004247:DNA / Q000032:analysis*; D004252:DNA Mutational Analysis / Q000592:standards; D004586:Electrophoresis / Q000379:methods* / Q000592:standards; D014644:Genetic Variation; D008967:Molecular Biology / Q000379:methods*; D010271:Parasites / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000592:standards; D018807:Polymorphism, Single-Stranded Conformational
D004247:DNA