Leukaemia inhibitory factor (LIF) production in a mouse model of spinal trauma.
A model of spinal trauma was developed where spinal neurones of adult mice were exposed to the excitotoxic glutamate analogue beta-N-oxylamino-L-alanine (L-BOAA). After 24 h, the injured neurones received a single dose of [125I]-LIF at the same site of the spinal cord, and 2 h later, tissues were removed to assess the distribution of leukaemia inhibitory factor (LIF). There was a significant increase in LIF binding to the injured region of the spinal cord over saline controls, and this corresponded with a significant increase in LIF mRNA expression in the same region of the cord. There was a change in the expression of ciliary neurotrophic factor, but the expression of cardiotrophin-1 (CT-1) and the common receptor subunit LIF receptor beta (LIFRbeta) did not change after neurotoxin treatment. The results add to the evidence that LIF plays a significant role in the response of adult neuronal tissue to injury.
Melbourne Neuromuscular Research Centre, St. Vincent's Hospital, Fitzroy, Australia.
J B
JB
Kurek
T M
TM
Bennett
J J
JJ
Bower
C M
CM
Muldoon
L
L
Austin
D000599:Amino Acids, Diamino; D000818:Animals; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000096:biosynthesis* / Q000378:metabolism; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; D008222:Lymphokines / Q000096:biosynthesis* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D016472:Motor Neuron Disease / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D009498:Neurotoxins / Q000633:toxicity; D012333:RNA, Messenger / Q000096:biosynthesis; D018121:Receptors, Cytokine / Q000096:biosynthesis / Q000378:metabolism; D053606:Receptors, OSM-LIF; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology; D013122:Spinal Diseases / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation; D015091:beta-Alanine / Q000031:analogs & derivatives / Q000633:toxicity
D000599:Amino Acids, Diamino; D020934:Ciliary Neurotrophic Factor; D016207:Cytokines; D006131:Growth Inhibitors; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D053680:Leukemia Inhibitory Factor Receptor alpha Subunit; C507838:Lif protein, mouse; C507841:Lifr protein, mouse; D008222:Lymphokines; D009419:Nerve Tissue Proteins; D009498:Neurotoxins; D012333:RNA, Messenger; D018121:Receptors, Cytokine; D053606:Receptors, OSM-LIF; D015091:beta-Alanine; C100126:oxalyldiaminopropionic acid; C092073:cardiotrophin 1