A reproducible, simple, and sensitive high-performance capillary electrophoresis method for simultaneous determination of capreomycin, ofloxacin and pasiniazide in urine.
Separation and determination of capreomycin (Cp), ofloxacin (Oflx) and pasiniazide (Ipa) in urine by high-performance capillary electrophoresis (HPCE) with 280 nm detection have been studied systematically. The calibration lines were linear in the range of 0.5 approximately 50 mg 1(-1), and the detection limits (S/N = 3) were 0.15, 0.20 and 0.10 mg 1(-1) for Cp, Oflx and Ipa, respectively. The recoveries for these materials from urine were higher than 93.5%. The accuracy and intra- and inter- day reproducibility of Cp, Oflx and Ipa were determined with satisfactory results. This method was successfully used for determining Cp. Oflx and Ipa in human urine.
Center of Instrumental Analysis, Zhengzhou University, P.R. China.
S S
SS
Zhang
H X
HX
Liu
Z B
ZB
Yuan
C L
CL
Yu
D010131:Aminosalicylic Acid / Q000652:urine; D000636:Aminosalicylic Acids; D002207:Capreomycin / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007538:Isoniazid / Q000031:analogs & derivatives* / Q000652:urine; D015242:Ofloxacin / Q000652:urine*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000636:Aminosalicylic Acids; D002207:Capreomycin; D010131:Aminosalicylic Acid; C008767:pasiniazide; D015242:Ofloxacin; D007538:Isoniazid