Expression of Kv1.1, a Shaker-like potassium channel, is temporally regulated in embryonic neurons and glia.
Kv1.1, a Shaker-like voltage-gated potassium channel, is strongly expressed in a variety of neurons in adult rodents, in which it appears to be involved in regulating neuronal excitability. Here we show that Kv1.1 is also expressed during embryonic development in the mouse, exhibiting two transient peaks of expression around embryonic day 9.5 (E9.5) and E14.5. Using both in situ hybridization and immunocytochemistry, we have identified several cell types and tissues that express Kv1.1 RNA and protein. At E9.5, Kv1.1 RNA and protein are detected transiently in non-neuronal cells in several regions of the early CNS, including rhombomeres 3 and 5 and ventricular zones in the mesencephalon and diencephalon. At E14.5, several cell types in both the CNS and peripheral nervous system express Kv1.1, including neuronal cells (sensory ganglia and outer aspect of cerebral hemispheres) and glial cells (radial glia, satellite cells, and Schwann cell precursors). These data show that Kv1.1 is expressed transiently in a variety of neuronal and non-neuronal cells during restricted periods of embryonic development. Although the functional roles of Kv1.1 in development are not understood, the cell-specific localization and timing of expression suggest this channel may play a role in several developmental processes, including proliferation, migration, or cell-cell adhesion.
The Virginia Merrill Bloedel Hearing Research Center and the Departments of Pharmacology and Otolaryngology-Head and Neck Surgery, University of Washington School of Medicine, Seattle, Washington 98195, USA.
J L
JL
Hallows
B L
BL
Tempel
D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005865:Gestational Age; D015640:Ion Channel Gating; D051662:Kv1.1 Potassium Channel; D008564:Membrane Potentials / Q000502:physiology; D051379:Mice; D008809:Mice, Inbred C3H; D009457:Neuroglia / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism*; D009928:Organ Specificity; D015221:Potassium Channels / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
C496947:Kcna1 protein, mouse; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D012333:RNA, Messenger; D051662:Kv1.1 Potassium Channel