Influence of some lysosomotropic compounds on calcium ion desorption process from liposome membrane.
The effect of a group of model lysosomotropic compounds on the process of Ca2+ ion desorption from lecithin liposome membranes was studied. The compounds studied were: hydrochlorides of fatty acids 2-dimethylaminoethyl esters (DM-n) for n = 9, 11, 13 and 15 carbon atoms in the fatty acid alkyl chain and methochloride of 2-dimethylaminoethyl laurate (DMS-11). It was found that all the compounds studied caused increased desorption with increasing concentration of the compound. Most effective was the quaternary ammonium salt, DMS-11. Moreover, it was found that the process of Ca2+ desorption from the membrane depended on pH of the medium. Compound DM-11 was more active at pH 8 than at pH 5. The action of DM-n compounds depended on the alkyl chain length, DM-11 and DM-13 being the most active. Apparently free amines penetrate the phospholipid membranes and incorporate into its hydrophobic core causing structural deformations. Hydrochlorides of fatty acids and the quaternary ammonium salt induce desorption of calcium ions mostly as a result of competitive electrostatic interactions. By quantum chemistry, PM3 method, and methods of molecular modelling we established the higher hydrophilicity of the polar head of DM-n series with respect to the polar head of the DMS-n compounds. DM-n compounds possess both acceptor and donor properties for hydrogen bonding while DMS-n are instrumental as acceptors only. It should be noted, that the results obtained in this paper for model membranes are in accordance with those for biological ones.
Kuczera
J
J
Department of Physics and Biophysics, Agricultural University, Wrocław, Poland. BIOPHYS@OZI.AR.WROC.PL
Witek
S
S
Kral
T E
TE
Hładyszowski
J
J
Luczyński
J
J
Przestalski
S
S
D002118:Calcium / Q000737:chemistry*; D005227:Fatty Acids / Q000737:chemistry*; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008247:Lysosomes / Q000378:metabolism*; D008954:Models, Biological; D008958:Models, Molecular; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry*
D005227:Fatty Acids; D008081:Liposomes; D010713:Phosphatidylcholines; D002118:Calcium