Bacteriocin inhibition of two glucose transport systems in Listeria monocytogenes.
Listeria monocytogenes transports glucose by proton motive force-mediated and phosphoenolpyruvate-dependent phosphotransferase systems (PEP-dependent PTS). Inhibition of both systems by nisin, pediocin JD and leuconosin S is reported here for four strains of L. monocytogenes. Intracellular and extracellular adenosine triphosphate (ATP) and extracellular inorganic phosphate were measured in energized L. monocytogenes Scott A cells to determine whether inhibition of the PEP-dependent PTS might occur as a result of bacteriocin-induced leakage of intracellular components. Addition of nisin resulted in a decrease in intracellular ATP with an increase in extracellular ATP. Leuconosin S and pediocin JD induced a depletion of intracellular ATP. ATP efflux was low for the leuconosin S-treated cells and barely detectable for pediocin JD-treated cells. Addition of nisin, leuconosin S and pediocin JD induced efflux of inorganic phosphate. It appears that bacteriocin-mediated inhibition of the glucose PEP-dependent PTS occurs as a result of hydrolysis or efflux of ATP, PEP and other essential molecules from L. monocytogenes cells.
Department of Food Science and Technology, University of Nebraska-Lincoln 68583-0919, USA.
B L
BL
Waite
G R
GR
Siragusa
R W
RW
Hutkins
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001430:Bacteriocins / Q000494:pharmacology*; D001693:Biological Transport, Active; D005947:Glucose / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000378:metabolism*; D009561:Nisin / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000378:metabolism; D018892:Proton-Motive Force; D013997:Time Factors
D001430:Bacteriocins; D010710:Phosphates; D009561:Nisin; D000255:Adenosine Triphosphate; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose