Determination of somatic mutant frequencies at glycophorin A and T-cell receptor loci for biodosimetry of prolonged irradiation.
OBJECTIVE
To determine the variant frequencies (VF) at glycophorin A (GPA) and T-cell receptor (TCR) loci in persons exposed to prolonged ionizing radiation at different doses and to assess the significance of the GPA and TCR assays for biodosimetry of prolonged irradiation.


METHODS
The VF values were determined by means of flow cytometry in 120 persons exposed between 1968-1996. Most exposures were in Chernobyl clean-up workers in 1986-1987.


RESULTS
A significant correlation was shown between the NO GPA variant cell frequency and dose (r = 0.61, p < 0.0001). The slope of the linear regression was 6.3 x 10(-6) NO mutant cells/Gy. Dose-dependent increase in the TCR VF was found in the group with recent exposures (slope 2.1 x 10(-4) variant cells/Gy, r = 0.75, p = 0.0002). In the Chernobyl clean up workers who received doses less than 0.25 Gy the TCR VF unlike the GPA VF was significantly higher than in the control non-irradiated individuals (p < 0.01 and p > 0.05 respectively).


CONCLUSIONS
The GPA assay has limited potential to be used as a biodosimeter of prolonged irradiation, at least in dose interval up to 2.0 Gy. The TCR assay is likely to have greater potential in estimation of recent radiation exposure than the GPA assay.
Department of Radiation Biochemistry, Medical Radiological Research Center of Russian Academy of Medical Sciences, Obninsk.
A S
AS
Saenko
I A
IA
Zamulaeva
S G
SG
Smirnova
N V
NV
Orlova
E I
EI
Selivanova
V A
VA
Saenko
N P
NP
Matveeva
M A
MA
Kaplan
V Y
VY
Nugis
N M
NM
Nadezhina
A F
AF
Tsyb
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D015704:CD4 Antigens / Q000235:genetics / Q000528:radiation effects; D002415:Cats; D005260:Female; D005434:Flow Cytometry; D014644:Genetic Variation; D006021:Glycophorins / Q000235:genetics* / Q000528:radiation effects; D006168:Guinea Pigs; D006801:Humans; D008951:MNSs Blood-Group System; D008297:Male; D016296:Mutagenesis; D011210:Power Plants; D011829:Radiation Dosage; D011834:Radiation Monitoring / Q000379:methods*; D018788:Radioactive Hazard Release; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000235:genetics* / Q000528:radiation effects; D012044:Regression Analysis; D014455:Ukraine
D015704:CD4 Antigens; D006021:Glycophorins; D008951:MNSs Blood-Group System; D017260:Receptor-CD3 Complex, Antigen, T-Cell