Structural homology between the Rap30 DNA-binding domain and linker histone H5: implications for preinitiation complex assembly.
The three-dimensional structure of the human Rap30 DNA-binding domain has been solved by multinuclear NMR spectroscopy. The structure of the globular domain is strikingly similar to that of linker histone H5 and its fold places Rap30 into the "winged" helix-turn-helix family of eukaryotic transcription factors. Although the domain interacts weakly with DNA, the binding surface was identified and shown to be consistent with the structure of the HNF-3/fork head-DNA complex. The architecture of the Rap30 DNA-binding domain has important implications for the function of Rap30 in the assembly of the preinitiation complex. In analogy to the function of linker histones in chromatin formation, the fold of the Rap30 DNA-binding domain suggests that its role in transcription initiation may be that of a condensation factor for preinitiation complex assembly. Functional similarity to linker histones may explain the dependence of Rap30 binding on the bent DNA environment induced by the TATA box-binding protein. Cryptic sequence identity and functional homology between the Rap30 DNA-binding domain and region 4 of Escherichia coli sigma70 may indicate that the sigma factors also possess a linker histone-like activity in the formation of a prokaryotic closed complex.
Groft
C M
CM
Laboratories of Molecular Biophysics, The Rockefeller University, New York, NY 10021, USA.
Uljon
S N
SN
Wang
R
R
Werner
M H
MH
D001483:Base Sequence; D004279:DNA, Viral; D004268:DNA-Binding Proteins / Q000737:chemistry*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry; D019077:Helix-Turn-Helix Motifs; D006657:Histones / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011487:Protein Conformation; D012808:Sigma Factor / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry*; D019473:Transcription Factors, TFII
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D006657:Histones; D012808:Sigma Factor; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; C056604:transcription factor TFIIF