Down-regulation of Fas-associated phosphatase-1 (FAP-1) in interleukin-2-activated T cells.
Peripheral T cells are resistant to Fas receptor (FasR/CD95)-mediated apoptosis. After prolonged treatment with interleukin-2 (IL-2), these T cells develop a Fas-sensitive phenotype. To clarify the molecular mechanism of apoptosis susceptibility, mRNA expression of FasR-associated proteins [Fas-associating protein with death domain (FADD), receptor-interacting protein (RIP), and Fas-associated phosphatase-1 (FAP-1)] has been investigated in IL-2 activated T cells. Competitive reverse transcriptase-polymerase chain reaction analysis revealed that FADD and RIP mRNA were equally expressed in freshly isolated resting T cells and IL-2-activated T cells. In contrast, FAP-1 mRNA was produced more abundantly by Fas-resistant resting T cells than by Fas-sensitive activated T cells. These findings suggested that sensitivity to FasR-mediated apoptosis in T cells could be correlated with down-regulation of FAP-1 expression. Additionally, CD45RO+ memory T cells expressed a larger amount of FAP-1 mRNA than did CD45RA+ naive T cells.
Department of Pediatrics, Mie University School of Medicine, Japan.
Y W
YW
Zhou
Y
Y
Komada
H
H
Inaba
E
E
Azuma
M
M
Sakurai
D048868:Adaptor Proteins, Signal Transducing; D017209:Apoptosis; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D002478:Cells, Cultured; D015536:Down-Regulation; D053222:Fas Ligand Protein; D053200:Fas-Associated Death Domain Protein; D015870:Gene Expression; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology; D054645:Protein Phosphatase 1; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000235:genetics; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; C505533:FADD protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D053200:Fas-Associated Death Domain Protein; D007376:Interleukin-2; D008562:Membrane Glycoproteins; D008934:Mitogens; D011506:Proteins; D012333:RNA, Messenger; C506249:RIPK1 protein, human; D053422:Receptor-Interacting Protein Serine-Threonine Kinases; D054645:Protein Phosphatase 1; C516742:PTPN13 protein, human; D054595:Protein Tyrosine Phosphatase, Non-Receptor Type 13; D017027:Protein Tyrosine Phosphatases