Analysis of cellular responses to native and recombinant proteins of Cowdria ruminantium.
The response of PBMC collected from cattle immunized with killed Cowdria to MAP1, MAP1-B, MAP2 and to a lysate of Cowdria was characterized in vitro. CD4+ T lymphocytes responded strongly to Cowdria lysates while B cells and gamma delta T cells proliferated in response to recombinant proteins. Short-term cell lines derived from PBMC using Cowdria lysates were more than 90% CD4+ and did not respond to any of the recombinant proteins. Restimulation of PBMC with MAP1, MAP1-B, and MAP2 proved difficult, and cultures usually stopped proliferating after 2 to 3 weeks. Only one cell line could be generated using MAP1 and it was composed of 85% gamma delta T cells. This study indicates that in animals immunized with killed Cowdria, proteins other than the serologically immunodominant MAP1 and MAP2 are strongly immunogenic for T lymphocytes.
Totté
P
P
CIRAD-EMVT, Point-à-Pitre, Gaudeloupe, French West Indies.
McKeever
D
D
Jongejan
F
F
Barbet
A
A
Mahan
S M
SM
Mwangi
D
D
Bensaid
A
A
D000818:Animals; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D016995:Ehrlichia ruminantium / Q000276:immunology*; D006357:Heartwater Disease / Q000276:immunology* / Q000517:prevention & control; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014614:Vaccines, Synthetic
D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001428:Bacterial Vaccines; C113540:MAP1-B protein, Cowdria ruminantium; C113541:MAP2 antigenic protein, Ehrlichia; D008565:Membrane Proteins; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; C086402:major antigenic protein 1, Cowdria ruminantium