Cultivation and grafting of human keratinocytes on a poly(hydroxyethyl methacrylate) support to the wound bed: a clinical study.
Cultured epithelial sheets on a textile support are used for the treatment of seriously burned patients. In this study we demonstrate a new procedure for the grafting of keratinocytes directly on a polymer cultivation support. This procedure is much easier in comparison with classical techniques, and encouraging results of clinical trials demonstrate the improved healing of the wound bed after the use of this procedure. There is no difference in the cytokeratine pattern (LP-34, cytokeratin-10) of the reconstructed epidermis and normal human skin.
Prague Burn Center, 3rd Faculty of Medicine, Charles University, Czech Republic.
B
B
Dvoránková
K
K
Smetana
R
R
Königová
H
H
Singerová
J
J
Vacík
M
M
Jelínková
Z
Z
Kapounková
M
M
Zahradník
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001672:Biocompatible Materials; D002056:Burns / Q000628:therapy*; D017690:Cell Transplantation / Q000379:methods; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000637:transplantation*; D008297:Male; D008875:Middle Aged; D011102:Polyhydroxyethyl Methacrylate; D014945:Wound Healing
D001672:Biocompatible Materials; D011102:Polyhydroxyethyl Methacrylate