Differential diagnosis of HIV type 1 group O and M infection by polymerase chain reaction and PstI restriction analysis of the pol gene fragment.
HIV-1 group O serological screening or confirmation strategies so far have not proved 100% sensitive and specific, indicating a lack of antibody reactivity or cross-reactivity with group O antigens. Therefore, genetic analysis currently represents the only method by which confirm presumed HIV-1 group O or group O/M infections. We have optimized the sensitivity (100%) and specificity (100%) of an HIV-1 group O/M-specific PCR of a pol gene fragment. In addition, we report on a highly sensitive (97.2%) and specific (100%) method for differentiation between HIV-1 group O and group M viruses, using PCR and PstI enzyme restriction fragment analysis of a pol fragment. Compared with sequencing, these methods are fast, inexpensive, and simple.
Heyndrickx
L
L
Institute of Tropical Medicine, Antwerp, Belgium.
Janssens
W
W
Gürtler
L
L
Zekeng
L
L
Loussert-Ajaka
I
I
Vereecken
K
K
Willems
B
B
Coppens
S
S
Ndumbe
P
P
Fransen
K
K
Saman
E
E
Alary
M
M
van der Groen
G
G
D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D015751:Genes, pol; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D008214:Lymphocytes / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D012680:Sensitivity and Specificity
D004279:DNA, Viral