Determination of quercetin in human plasma by HPLC with spectrophotometric or electrochemical detection.
A reversed-phase high-performance liquid chromatographic method for the determination of quercetin in human plasma following intravenous infusion is described. Quercetin in plasma was extracted with methanol-dimethyl sulphoxide (4:1 v/v) and separated on a C18 Hypersil-BDS column with 44% (v/v) methanol in 0.1 M ammonium acetate (pH 5.15) containing 0.27 mM EDTA as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 375 nm, or electrochemically, with a detection limit of 80 ng/mL and 2 ng/mL, respectively.
MRC Toxicology Unit, University of Leicester, UK.
D J
DJ
Jones
C K
CK
Lim
D R
DR
Ferry
A
A
Gescher
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007262:Infusions, Intravenous; D011794:Quercetin / Q000008:administration & dosage / Q000097:blood*; D013056:Spectrophotometry, Ultraviolet
D011794:Quercetin