Myostatin maps to porcine chromosome 15 by linkage and physical analyses.
Myostatin belongs to the transforming growth factor-beta superfamily, and is expressed specifically in developing and mature skeletal muscle. Myostatin appears to act as a negative regulator of muscle development, since mice with targeted disruption of this gene display a large increase in muscle mass. In this study, the porcine myostatin gene was mapped to chromosome 15q2.3 by fluorescence in situ hybridization. Myostatin was also positioned within the chromosome 15 linkage group using both a polymorphism located in the second intron and an associated microsatellite. The development of highly polymorphic markers associated with myostatin will support population studies to identify alleles of this gene that affects muscle mass and/or fat deposition in swine.
Sonstegard
T S
TS
USDA, ARS, U.S. Meat Animal Research Center (MARC), Clay Center, NB 68933, USA.
Rohrer
G A
GA
Smith
T P
TP
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D018244:Chromosomes, Artificial, Yeast / Q000737:chemistry; D017931:DNA Primers; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics; D018482:Muscle, Skeletal / Q000503:physiopathology*; D055435:Myostatin; D012150:Polymorphism, Restriction Fragment Length; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D013552:Swine / Q000235:genetics* / Q000502:physiology; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D005819:Genetic Markers; C525912:Mstn protein, mouse; D055435:Myostatin; D016212:Transforming Growth Factor beta