Novel p53 tumour suppressor mutations in cases of spindle cell sarcoma, pleomorphic sarcoma and fibrosarcoma in cats.
Twenty feline neoplasms were sequenced in the region from exons 5 to 8 for the presence of tumour suppressor gene p53 mutations. In a spindle cell sarcoma of the bladder, a missense mutation (codon 164 AAG-->GAG, lysine-->glutamic acid) in exon 5 was detected. In a pleomorphic sarcoma, a 23 bp deletion involving the splicing junction between intron 5 and exon 6 was observed. In a fibrosarcoma, a 6 bp deletion of p53 covering 2 bp of exon 7 and 4 bp of intron 7, including the splicing junction, was found. The study demonstrates three new p53 mutations in different types of sarcomas in cats.
Mayr
B
B
Institute for Animal Breeding and Genetics, Veterinary University, Vienna, Austria.
Reifinger
M
M
Alton
K
K
Schaffner
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000235:genetics* / Q000473:pathology; D002415:Cats; D004273:DNA, Neoplasm / Q000737:chemistry; D005260:Female; D005354:Fibrosarcoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary; D017353:Gene Deletion; D016158:Genes, p53 / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012509:Sarcoma / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D017422:Sequence Analysis, DNA; D012878:Skin Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000473:pathology / Q000662:veterinary*
D004273:DNA, Neoplasm