Adeno-associated virus-mediated gene transfer into human retinal pigment epithelium cells.
OBJECTIVE
Adeno-associated virus (AAV) is emerging as a promising vector for gene therapy


METHODS
To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV-GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neo(r)) genes was constructed and used to transduce RPE 407A cell line.


RESULTS
Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months).


CONCLUSIONS
Adeno-associated virus-based vectors are able to efficiently transduce and stably persist in RPE cells.
Centre for Ophthalmology and Visual Science, University of Western Australia, Perth, Australia. ylai@cyllene.uwa.edu.au
Y K
YK
Lai
P
P
Rakoczy
I
I
Constable
F
F
Rolling
D002460:Cell Line; D002478:Cells, Cultured; D003673:Defective Viruses; D000229:Dependovirus / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism; D010857:Pigment Epithelium of Eye / Q000378:metabolism* / Q000821:virology; D010957:Plasmids; D011993:Recombinant Fusion Proteins
D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins