Effects of interleukin-1 beta on human pulpal fibroblast proliferation and collagen synthesis.
The aim of this study was to investigate the effect of interleukin-1 beta (IL-1 beta) on pulpal fibroblast proliferation and collagen synthesis in vitro. Cell proliferation was assessed by [3H]thymidine uptake at 24, 48, and 72 h. These cells were compared with dermal fibroblasts under a range of conditions. IL-1 beta inhibited pulp cell proliferation, but this effect was decreased by the presence of indomethacin. In contrast, IL-1 beta stimulated dermal fibroblast proliferation in the absence and presence of indomethacin. Collagen synthesis was analyzed by incorporation of 5-[3H]proline into polypeptide chains. IL-1 beta stimulated synthesis of type I collagen both in the absence and presence of indomethacin. Results suggest that the inhibitory effect on pulp cell proliferation is dependent upon IL-1 beta-induced prostaglandin E2 synthesis and that IL-1 beta is a potent mediator of prostaglandin E2 synthesis in dental pulp. These observations imply a role of IL-1 beta in collagen synthesis, but not in the fibroblast proliferation phase of the healing process.
School of Dental Science, University of Melbourne, Victoria, Australia.
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V
Lertchirakarn
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R
Birner
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HH
Messer
D000704:Analysis of Variance; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003094:Collagen / Q000096:biosynthesis*; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D018836:Inflammation Mediators / Q000494:pharmacology*; D007375:Interleukin-1 / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D018836:Inflammation Mediators; D007375:Interleukin-1; D003094:Collagen; D007213:Indomethacin