Supernumerary ring chromosomes and nuclear blebs in some low-grade malignant soft tissue tumours: atypical lipomatous tumours and dermatofibrosarcoma protuberans.
We investigated the diagnostic significance of supernumerary ring chromosomes in low-grade soft-tissue neoplasms. Chromosome slides were prepared from 123 samples of soft-tissue tumours using the standard trypsin-Giemsa banding technique. Supernumerary ring chromosomes were found in 6 cases of soft tissue tumours: 5 cases of atypical lipomatous tumour (ALT) and 1 case of dermatofibrosarcoma protuberans (DFSP). By chromosome painting with fluorescence in situ hybridization (FISH), the ring chromosome in 1 ALT was painted over its entire length with the chromosome 12 probe. Nuclear blebs and micronuclei, which were observed in each case of ALT, also contained chromosome 12 material; and these structures may represent a topological distribution of ring or giant marker chromosomes in the interphase nuclei. Our findings suggest that supernumerary ring chromosomes are characteristic of some low-grade soft tissue neoplasms including ALT and DFSP.
Iwasaki
H
H
Department of Pathology, Fukuoka University School of Medicine, Japan. iwasakih@msat.fukuoka-u.ac.jp
Ohjimi
Y
Y
Ishiguro
M
M
Isayama
T
T
Fujita
C
C
Kaneko
Y
Y
Kikuchi
M
M
Shinohara
N
N
D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000473:pathology*; D002881:Chromosomes, Human, Pair 12; D018223:Dermatofibrosarcoma / Q000235:genetics / Q000473:pathology*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007564:Japan; D007621:Karyotyping; D008067:Lipoma / Q000235:genetics / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012303:Ring Chromosomes; D012983:Soft Tissue Neoplasms / Q000235:genetics / Q000473:pathology*