Controlled release of platelet-derived growth factor from porous poly(L-lactide) membranes for guided tissue regeneration.
Platelet-derived growth factor-BB (PDGF-BB) was incorporated into porous poly (L-lactide) (PLLA) membranes with an aim of improving early bone healing in guided tissue regeneration (GTR) therapy. Porous PDGF-BB loaded membranes were fabricated by coating PDGF-BB-dissolved PLLA methylene chloride-ethyl acetate solutions on polyglycolic acid (PGA) meshes. Release kinetics of PDGF-BB, biologic activity, degradability and guided tissue regenerative potentials of the membranes were investigated. Release of PDGF-BB could be controlled by adding bovine serum albumin that may provide porous diffusion channels for PDGF-BB release and by varying initial loading content of PDGF-BB. Biologic activity of PDGF-BB in the membranes was ascertained by fibroblast chemotaxis. PDGF-BB loaded membranes maintained proper degradation property for periodontal application. PDGF-BB loaded membrane markedly increased new bone formation in rat calvarial defects, and completed bony reunion after 2 weeks of implantation period. These results suggested that PDGF-BB loaded PLLA membrane might potentially enhance guided tissue regenerative efficacy.
Department of Industrial Pharmacy, College of Pharmacy, Ewha Womans University, Seoul, South Korea.
Y J
YJ
Park
Y
Y
Ku
C P
CP
Chung
S J
SJ
Lee
D000085:Acetates; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects*; D004396:Coloring Agents; D003692:Delayed-Action Preparations; D005881:Gingiva; D016557:Guided Tissue Regeneration, Periodontal / Q000379:methods; D066298:In Vitro Techniques; D008567:Membranes, Artificial; D008752:Methylene Chloride; D008855:Microscopy, Electron, Scanning; D010982:Platelet-Derived Growth Factor / Q000032:analysis* / Q000494:pharmacology; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull; D013499:Surface Properties
D000085:Acetates; D001672:Biocompatible Materials; D004396:Coloring Agents; D003692:Delayed-Action Preparations; D008567:Membranes, Artificial; D010982:Platelet-Derived Growth Factor; D011091:Polyesters; D011100:Polyglycolic Acid; C033616:poly(lactide); D008752:Methylene Chloride; C007650:ethyl acetate