Increased acute and chronic mitotic activity in rat laryngeal muscles after botulinum toxin injection.
OBJECTIVE
To characterize the acute and chronic cellular effects of botulinum toxin (BT) injection into rat laryngeal muscles. A complete characterization of these effects is important because patients with focal dystonias of the head and neck are commonly treated with BT injection. Further, potential muscular changes in the larynx must be carefully delineated owing to the critical phonatory and airway protective functions of these muscles.


METHODS
The acute and chronic cellular effects of BT injection were studied using 5'-bromo 2'-deoxyuridine (BrdU) following single and repeated BT injection into rat laryngeal muscles. BrdU is incorporated into mitotically active nuclei such that changes in cell proliferative behavior following BT injection can be monitored.


RESULTS
Increased mitotic activity was detected in the tissue samples studied following BT injection. Differences in the times of the peak distribution of BrdU-labeled cells in each laryngeal muscle were observed. This may be related to the diffusion effects of BT. Prolonged muscle fiber changes, including splitting, were also observed as the result of repeated BT injection.


CONCLUSIONS
The results of this study suggest that BT may induce a proliferative response in muscle tissue.
Division of Otolaryngology--Head and Neck Surgery, University of Wisconsin Medical School, Madison, USA.
K
K
Inagi
N P
NP
Connor
E
E
Schultz
C N
CN
Ford
C H
CH
Cook
D M
DM
Bless
D M
DM
Heisey
D000818:Animals; D018726:Anti-Dyskinesia Agents / Q000494:pharmacology*; D001764:Blepharospasm / Q000188:drug therapy; D001905:Botulinum Toxins / Q000494:pharmacology*; D001973:Bromodeoxyuridine; D002455:Cell Division / Q000187:drug effects; D004334:Drug Administration Schedule; D004353:Drug Evaluation, Preclinical; D007273:Injections, Intramuscular; D007821:Laryngeal Muscles / Q000166:cytology* / Q000187:drug effects*; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014832:Voice Disorders / Q000188:drug therapy
D018726:Anti-Dyskinesia Agents; D001905:Botulinum Toxins; D001973:Bromodeoxyuridine