Rapid characterization of artemether and its in vitro metabolites on incubation with bovine hemoglobin, rat blood and dog blood by capillary gas chromatography-chemical ionization mass spectrometry.
A fast and sensitive analytical method was developed to characterize artemether and its metabolites in small amounts in body fluids. The extracts were derivatized with N-methyl-N-trimethylsilyltrifluoroacetamide, separated on an optimized capillary gas chromatographic system and identified by chemical ionization mass spectrometry by using ammonia as reagent gas. The analytical assay is demonstrated on samples extracted from bovine hemoglobin, rat blood and dog blood. Full mass spectra of artemether and three metabolites were obtained at a level of 1 x 10(-6) g/ml.
Blum
W
W
Novartis Pharma AG, Research Department, Basel, Switzerland.
Pfaar
U
U
Kühnöl
J
J
D000818:Animals; D000962:Antimalarials / Q000097:blood*; D000077549:Artemether; D037621:Artemisinins; D002384:Catalysis; D002417:Cattle; D004285:Dogs; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D007501:Iron / Q000737:chemistry; D008297:Male; D051381:Rats; D012717:Sesquiterpenes / Q000097:blood*
D000962:Antimalarials; D037621:Artemisinins; D012717:Sesquiterpenes; D000077549:Artemether; D007501:Iron