Identification and sequence analyses of the granulin gene of Choristoneura fumiferana granulovirus.
The nucleotide sequence of the granulin gene of Choristoneura fumiferana granulovirus (CfGV) was determined. The gene encodes a protein of 248 amino acids with a predicted Mr of 29.299 kDa. The granulin genes of Trichoplusia ni, Pieris brassicae and Cryptophlebia leucotreta granuloviruses showed homologies ranging from 76.7-80.5% for nucleotide sequences and 84.2-88.3% for amino acid sequences when compared to CfGV. The secondary structure of CfGV granulin protein, including the hydrophilic (polar) and hydrophobic (basic) regions, was predicted and found to be similar to other granulins. A very late baculovirus promoter motif, ATAAG, was found within the putative promoter region of the CfGV granulin gene.
Institut Armand-Frappier, Centre de Recherche en Virologie, Laval, Québec, Canada.
A
A
Bah
J
J
Bergeron
M
M
Arella
C J
CJ
Lucarotti
C
C
Guertin
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D001483:Base Sequence; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014763:Viral Matrix Proteins / Q000235:genetics*
D014763:Viral Matrix Proteins