Effects of a vegetables-fruit mixture on liver and colonic 1,2-dimethylhydrazine-metabolizing enzyme activities in rats fed low- or high-fat diets.
The effects of a vegetables-fruit mixture (19.5% w/w) were studied on hepatic (h) and colonic (c) 1,2-dimethylhydrazine (DMH)-metabolizing enzyme activities (ethoxy- and pentoxyresorufine-O-deethylation, NDMA-demethylase, glutathione-S-transferase, UDP-glucuronyltransferase) in rats fed low- or high-fat diets (20 or 40 energy%). A vegetables-fruit mixture added to the diets resulted in altered enzyme activities in animals either treated or not treated with DMH. Remarkably, the vegetables-fruit mixture given to DMH-treated rats decreased glutathione-S-transferase (h) and increased NDMA-demethylase activities (c), whereas the mixture given to controls increased glutathione-S-transferase (h) and decreased NDMA-demethylase activities (c). The high-fat diets only modulated enzyme activities in animals not treated with DMH.
Department of Toxicology, Wageningen Agricultural University, The Netherlands. office@algemeen.tox.wau.nl
J M
JM
Rijnkels
G M
GM
Alink
D019813:1,2-Dimethylhydrazine / Q000378:metabolism*; D000818:Animals; D002273:Carcinogens / Q000378:metabolism*; D003106:Colon / Q000201:enzymology*; D019363:Cytochrome P-450 CYP1A1 / Q000187:drug effects / Q000378:metabolism; D019362:Cytochrome P-450 CYP2B1 / Q000187:drug effects / Q000378:metabolism; D019392:Cytochrome P-450 CYP2E1 / Q000187:drug effects / Q000378:metabolism; D004041:Dietary Fats / Q000008:administration & dosage*; D005638:Fruit; D014453:Glucuronosyltransferase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D014675:Vegetables
D002273:Carcinogens; D004041:Dietary Fats; D019392:Cytochrome P-450 CYP2E1; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D014453:Glucuronosyltransferase; D005982:Glutathione Transferase; D019813:1,2-Dimethylhydrazine