Characterisation of duck thrombocytes.
Duck thrombocytes were initially identified in peripheral blood mononuclear cells (PBMCs) purified from whole blood on Ficoll-Paque density gradients by examining stained smears of these cells. These thrombocytes could be readily purified from lymphocytes on the FACStar cell sorter by their increased side-scatter. They were similar to chicken thrombocytes in both appearance and function; they had a diameter of 4.5-6 microm and contained large vacuoles and were able to phagocytose carbon and Staphylococcus aureus. A monoclonal antibody (mAb) BA3 was generated which binds specifically to duck thrombocytes and has facilitated the characterisation of these cells which comprise up to 50-60 per cent of cells in Ficoll-Paque purified duck PBMCs.
Department of Microbiology and Immunology, The University of Adelaide, SA, Australia.
E M
EM
Bertram
A R
AR
Jilbert
I
I
Kotlarski
D000818:Animals; D000911:Antibodies, Monoclonal; D001792:Blood Platelets / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure; D002244:Carbon; D002469:Cell Separation / Q000379:methods; D002645:Chickens; D004372:Ducks / Q000097:blood*; D005434:Flow Cytometry; D010587:Phagocytosis; D013211:Staphylococcus aureus; D014617:Vacuoles / Q000648:ultrastructure
D000911:Antibodies, Monoclonal; D002244:Carbon