Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors.
We recently identified and characterized a novel murine gene, ENC-1, that is expressed primarily in the nervous system and encodes an actin-binding protein. To gain insight into a potential role for ENC-1 gene in the processes of cell differentiation and malignant transformation in the human nervous system, we first cloned and characterized the human homologue of ENC-1. The human ENC-1 gene appeared to be highly expressed in adult brain and spinal cord, and in a number of cell lines derived from nervous system tumors we detected low steady-state levels of ENC-1 mRNA. We used a neuroblastoma differentiation model, the retinoic acid-induced neuronal differentiation of SMS-KCNR cells, to study the regulation of the ENC-1 gene during neural crest cell differentiation. We found that the expression of ENC-1 increased dramatically in the differentiated SMS-KCNR cells as compared to control undifferentiated cells. These results suggest that ENC-1 expression plays a role during differentiation of neural crest cells and may be down regulated in neuroblastoma tumors.
Department of Neurological Surgery, School of Medicine, Brain Tumor Research Center, HSE 722, 513 Parnassus Avenue, San Francisco, California, 94143-0520, USA.
M C
MC
Hernandez
P J
PJ
Andres-Barquin
I
I
Holt
M A
MA
Israel
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D002490:Central Nervous System / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009423:Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology; D009479:Neuropeptides; D009687:Nuclear Proteins; D010179:Pancreas / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013116:Spinal Cord / Q000378:metabolism; D014018:Tissue Distribution; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D008840:Microfilament Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D012333:RNA, Messenger; C112302:ectodermal-neural cortex 1 protein; D014212:Tretinoin; D003994:Bucladesine