Identification of sucrose synthase as an actin-binding protein.
Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.
US Department of Agriculture, Agricultural Research Service, Raleigh, NC, USA.
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Winter
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Huber
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Huber
D000199:Actins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003902:Detergents; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D017830:Octoxynol; D010766:Phosphorylation; D011817:Rabbits; D012710:Serum Albumin, Bovine; D012995:Solubility; D003313:Zea mays / Q000201:enzymology
D000199:Actins; D003902:Detergents; D008840:Microfilament Proteins; D012710:Serum Albumin, Bovine; D017830:Octoxynol; D005964:Glucosyltransferases; C022699:sucrose synthase; D005634:Fructose-Bisphosphate Aldolase