Expression of intercellular adhesion molecule-1 in mice with Pseudomonas-induced pyelonephritis.
OBJECTIVE
To investigate the role of intercellular adhesion molecule-1 (ICAM-1) in the renal inflammatory process, we studied the time-course fluctuation of ICAM-1 expression on inflammatory lesions in mice with experimentally induced bacterial pyelonephritis and the effect of in vivo administration of an anti-ICAM-1 monoclonal antibody (mAb) on leukocytic migration.


METHODS
Ascending pyelonephritis was induced by transurethral instillation of Pseudomonas aeruginosa, and the expression of ICAM-1 in the pyelonephritic lesions was studied by immunohistochemical methods.


RESULTS
The expression of ICAM-1 on the pyelonephritic lesions closely paralleled the degree of infiltration of neutrophils and macrophages until 3 days after infection. At 7 days after infection, though the degree of infiltration of these cells was quite high, expression of ICAM-1 was reduced. Treatment with the anti-ICAM-1 mAb in mice with bacterial pyelonephritis resulted in suppression of influx of neutrophils and macrophages in the infected sites until 3 days after infection. However, at 7 days after infection inhibition of the influx of these cells was not seen.


CONCLUSIONS
These results suggest that ICAM-1 expression is transient and plays a key role in the influx of neutrophils and macrophages associated with the early-phase response, and that in the late phase ICAM-1 independent adhesion molecules may be more predominant.
Department of Urology, Sapporo Medical University School of Medicine, Japan.
A
A
Yokoo
T
T
Hirose
M
M
Matsukawa
H
H
Hotta
Y
Y
Kunishima
S
S
Takahashi
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000627:therapeutic use; D002465:Cell Movement / Q000276:immunology; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D015169:Colony Count, Microbial; D004727:Endothelium / Q000276:immunology; D005260:Female; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D018836:Inflammation Mediators / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics* / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D011552:Pseudomonas Infections / Q000473:pathology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D011704:Pyelonephritis / Q000382:microbiology* / Q000473:pathology
D000911:Antibodies, Monoclonal; D018836:Inflammation Mediators; D018799:Intercellular Adhesion Molecule-1