A radioimmunoassay combined with solid-phase extraction for the determination (pg ml-1) of AR-C15849XX) in human plasma.
A radioimmunoassay has been developed for the determination of AR-C15849KF, a CCK-8 analogue, in human plasma. The method incorporates solid-phase sample extraction, is suitable for the determination of the analyte at pg ml-1 concentrations and is based on a method developed and validated for dog plasma. The solid-phase extraction, using ion-exchange aminopropyl and octadecyl sorbents sequentially, was retained for this procedure to remove matrix interferences in the plasma and to enhance method sensitivity. The calibration range is 10-500 pg ml-1, using a 1 ml sample of undiluted human plasma. The method has been successfully used to generate early human pharmacokinetic data during a programme of exploratory development.
Department of Physical and Metabolic Sciences, Astra Charnwood, Loughborough, UK.
V J
VJ
Willson
J R
JR
Preston
W J
WJ
Lockley
J
J
Singh
D
D
Wilkinson
D000818:Animals; D001067:Appetite Depressants / Q000032:analysis*; D004285:Dogs; D006801:Humans; D011863:Radioimmunoassay; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012844:Sincalide / Q000031:analogs & derivatives* / Q000097:blood
C114705:AR C15849KF; D001067:Appetite Depressants; D012844:Sincalide