Determination of netilmicin sulfate by liquid chromatography with pulsed electrochemical detection.
The determination of netilmicin sulfate by liquid chromatography using a column packed with poly (styrene-di-vinylbenzene) and pulsed electrochemical detection on a gold electrode is described. The mobile phase consisted of an aqueous solution containing 35 g 1-1 of sodium sulfate, 0.5 g 1-1 of sodium octanesulfonate, 10 ml 1-1 of tetrahydrofuran and 50 ml 1-1 of 0.2 M phosphate buffer (pH 3.0). The total analysis time was not more than 25 min. The effects of the different chromatographic parameters on this selection were also investigated. When a number of commercial samples of netilmicin sulfate was analyzed using this method, eight different components were separated, three of which were of unknown identities.
Laboratorium voor Farmaceutische Chemie en Analyse van Geneesmiddelen, Faculteit Farmaceutische Wetenschappen, Katholieke Universiteit Leuven, Belgium.
E
E
Adams
D
D
Puelings
M
M
Rafiee
E
E
Roets
J
J
Hoogmartens
D002853:Chromatography, Liquid; D004563:Electrochemistry; D005839:Gentamicins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009428:Netilmicin / Q000032:analysis*; D015203:Reproducibility of Results; D012997:Solvents
D005839:Gentamicins; D007202:Indicators and Reagents; D012997:Solvents; D009428:Netilmicin