Increase in brain stem cytokine mRNA levels as an early response to chemical-induced myelin edema.
This study examined the early response of pro-inflammatory and regulatory cytokines in the mouse brain following triethyltin (TET)-induced myelin injury characterized by edematous vacuolation. Following an acute intraperitoneal injection of triethyltin (TET) sulfate (3 mg/kg) to 17-day old CD1 mice, significant increases in brain stem TNF-alpha and IL-1alpha mRNA levels occurred at 6 and 24 h, respectively with elevations in TGF-beta1 and MIP-1alpha at 1 h. In the cortex, responses were limited to elevations at 6 h in TNF-alpha, TGF-beta1 and MIP-1alpha. These data suggest that a chemokine/cytokine response can occur with minimal alterations to the integrity of the myelin sheath and may contribute to the initial signaling mechanisms associated with demyelinating disorders.
Department of Chemistry, University of North Carolina at Chapel Hill, 27599, USA.
P S
PS
Mehta
A
A
Bruccoleri
H W
HW
Brown
G J
GJ
Harry
D000818:Animals; D001929:Brain Edema / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines / Q000235:genetics*; D017403:In Situ Hybridization; D019402:Macrophage Inflammatory Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism*; D012260:Ribonucleases; D016212:Transforming Growth Factor beta / Q000235:genetics; D014267:Triethyltin Compounds / Q000494:pharmacology
D054405:Chemokine CCL3; D054407:Chemokine CCL4; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D014267:Triethyltin Compounds; C031209:Triethyltin sulfate; D012260:Ribonucleases