Expression of vascular endothelial growth factor in human gallbladder lesions.
The purpose of this study was to examine whether vascular endothelial growth factor (VEGF) regulates angiogenesis in human gallbladder carcinomas. We examined the expression of VEGF in 131 gallbladder lesions by immunohistochemistry and in situ mRNA hybridization technique. VEGF immunoreactivity was detected in 100% (9/9) of hyperplasias, 75% (3/4) of adenomas and 91% (107/118) of carcinomas. In gallbladder carcinoma tissues, VEGF mRNA level directly correlated with its protein level. VEGF expression in the neoplasms significantly correlated with vascularization, but not prognostic parameters. These findings suggest that VEGF is commonly expressed in human gallbladder carcinomas and may contribute to neovascularization during carcinogenesis.
First Department of Internal Medicine, Hiroshima University School of Medicine, Hiroshima 734-8551, Japan.
S
S
Yamamoto
K
K
Fujii
Y
Y
Kitadai
S
S
Yamamoto
A
A
Tsuchida
G
G
Kajiyama
D000236:Adenoma / Q000098:blood supply / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D002196:Capillaries / Q000473:pathology; D016228:Endothelial Growth Factors / Q000032:analysis / Q000096:biosynthesis*; D005704:Gallbladder / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology*; D005706:Gallbladder Neoplasms / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D006801:Humans; D006965:Hyperplasia; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis*; D009361:Neoplasm Invasiveness; D009389:Neovascularization, Pathologic; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors