Synthesis of gamma-phosphate-linked nucleoside affinity chromatography resins for protein purification, including ribonucleoside triphosphate reductase.
Seven nucleotides linked through the gamma-phosphate to diamine hydrocarbons were synthesized and coupled to Sepharose for use in protein purification affinity chromatography. The synthesis involved converting the nucleotides to nucleoside-5'- trimetaphosphates using dicyclohexyl carbodiimide, followed by nucleophilic ring opening of the trimetaphosphate with an alpha, omega-diamino hydrocarbon to generate a gamma-phosphoamide linkage in each nucleotide.
Suto
R K
RK
Department of Biochemistry, Colorado State University, Fort Collins 80523, USA.
Whalen
M A
MA
Bender
B R
BR
Finke
R G
RG
D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods; D009705:Nucleosides; D010710:Phosphates; D011506:Proteins / Q000302:isolation & purification*; D012116:Resins, Plant / Q000138:chemical synthesis* / Q000737:chemistry*; D012264:Ribonucleotide Reductases / Q000302:isolation & purification*
D009705:Nucleosides; D010710:Phosphates; D011506:Proteins; D012116:Resins, Plant; D012264:Ribonucleotide Reductases; C057295:ribonucleoside-triphosphate reductase