Clarification of HLA-B serologically ambiguous types by automated DNA sequencing.
Assignment of HLA-B types can be hampered by ambiguous reactivity of the typing sera resulting in inaccurate HLA-B assignments. In this study, 19 Korean samples exhibiting ambiguous serologic reactivities were characterized by DNA sequencing. Alleles identified from 7 samples were previously undetected in this population (B*1517, B*4101, B*4701, B*5001, and B*5106) and from 9 samples were common alleles in this population (B*4002, B*4003, B*4006, B*1501, B*1401, B*67012, and B*5401). Three samples were putative HLA-B homozygotes. Three major factors causing serologic ambiguity were identified: weak or false negative reactivity of typing sera (52.4%); cross or false positive reactivity of the sera (38.1%); and absence of information on the reaction patterns due to the lack of appropriate sera in the typing kit (e.g. B*4101 encoded molecule) or to the presence of recently characterized molecules (e.g. B*5106 encoded molecule) (9.5%). Overall, sequencing was helpful in clarifying ambiguous serologic reaction patterns improving the HLA typing for the Korean population.
Lee
K W
KW
Department of Clinical Pathology, College of Medicine, Hallym University, Seoul, Korea. leekw@www.hallym.or.kr
Steiner
N
N
Hurley
C K
CK
D000483:Alleles; D001331:Automation; D003429:Cross Reactions; D005188:False Negative Reactions; D005189:False Positive Reactions; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics* / Q000276:immunology; D006650:Histocompatibility Testing; D017422:Sequence Analysis, DNA
D015235:HLA-B Antigens