Properties of calpastatin forms in rat brain.
Four recombinant calpastatin forms, deduced from rat brain mRNAs and differing in the number of inhibitory repetitive domains from zero to four, were expressed and characterized for their inhibitory efficiency on mu- and m-calpain. Although the most effective one is a truncated calpastatin form composed of the N-terminal region (domain L) and a single inhibitory domain, all inhibitors are more active against mu-calpain, but are preferentially degraded and inactivated by m-calpain. The protein form composed exclusively of a domain L is deprived of any inhibitory activity but prevents inhibition of calpain by the other calpastatin forms, indicating that this calpastatin region could be relevant in the recognition of the proteinase. A calpastatin form having molecular properties similar to those of the recombinant truncated calpastatin, has also been found in rat brain. It does not derive from proteolysis of a higher molecular mass precursor. The expression of multiple calpastatin forms may be relevant for the specific modulation of the different calpain isozymes normally present in a single cell type.
Institute of Biological Chemistry, University of Genoa, Italy. melloni@csita.unige.it
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E
Melloni
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De Tullio
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M
Averna
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I
Tedesco
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F
Salamino
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Sparatore
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Pontremoli
D000818:Animals; D001921:Brain / Q000502:physiology*; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002154:Calpain / Q000037:antagonists & inhibitors / Q000378:metabolism; D003001:Cloning, Molecular; D004791:Enzyme Inhibitors / Q000378:metabolism; D004926:Escherichia coli; D012333:RNA, Messenger; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism
D002135:Calcium-Binding Proteins; D004791:Enzyme Inhibitors; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C033668:calpastatin; D002154:Calpain