Nicotiana benthamiana plants expressing beet necrotic yellow vein virus (BNYVV) coat protein-specific scFv are partially protected against the establishment of the virus in the early stages of infection and its pathogenic effects in the late stages of infection.
Transgenic plants of Nicotiana benthamiana expressing single chain antibody fragments (scFv) specific for the coat protein of beet necrotic yellow vein virus (BNYVV) and non-expressing control plants were inoculated with BNYVV mechanically and by means of the vector Polymyxa betae. The scFv were presumably expressed in the endoplasmic reticulum (ER). The average time needed for infections to become detectable was longer in the scFv-expressing plants than in the non-expressing control plants. In addition, the scFv-expressing plants were partially protected against the pathogenic effects exerted by the virus on N. benthamiana plants in the late stages of infection.
Fecker
L F
LF
Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.
Koenig
R
R
Obermeier
C
C
D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D002213:Capsid / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology; D004721:Endoplasmic Reticulum / Q000276:immunology; D007128:Immunoglobulin Fragments / Q000096:biosynthesis* / Q000276:immunology; D007135:Immunoglobulin Variable Region / Q000096:biosynthesis / Q000276:immunology; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000276:immunology* / Q000502:physiology; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D012328:RNA Viruses / Q000276:immunology* / Q000502:physiology; D011994:Recombinant Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000276:immunology / Q000821:virology*
D000914:Antibodies, Viral; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D011994:Recombinant Proteins; C055966:immunoglobulin Fv