Single-cell measurements of human neutrophil activation using electrorotation.
We have used the technique of cell electrorotation within a microfabricated planar gold electrode array as a means of detecting changes in the physical properties of a single human neutrophil, when activated by the chemotactic factor, phorbol myristate acetate (PMA). The results, which have been analyzed using a double-shell model to represent the cell and its nucleus, provide an indication of the changes in biophysical parameters that occur during cell activation. The methods used in this study have potential applications in the development of single-cell assays for pharmaceutical screening, as a means of determining rapidly the action of drugs.
Griffith
A W
AW
Department of Electronics & Electrical Engineering, Glasgow University, U.K.
Cooper
J M
JM
D002448:Cell Adhesion / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D004574:Electromagnetic Fields; D006801:Humans; D018375:Neutrophil Activation; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology; D013755:Tetradecanoylphorbol Acetate
D013755:Tetradecanoylphorbol Acetate