Identification of a rough strain of Escherichia coli O157:H7 that produces no detectable O157 antigen.
MA6, an O157:H7-like strain, did not react with most anti-O157 kits examined; however, it had the rfbE gene that is essential for O157 expression and carried O157:H7 virulence factors. Lipopolysaccharide analysis showed that MA6 is a rough strain that does not produce the O157 antigen, but genetically, it belongs in the O157:H7 clonal group.
Division of Microbiological Studies, Food and Drug Administration, Washington, D.C. 20204, USA. pxf@fdacf.ssw.dhhs.gov
P
P
Feng
R C
RC
Sandlin
C H
CH
Park
R A
RA
Wilson
M
M
Nishibuchi
D000818:Animals; D015373:Bacterial Typing Techniques; D002238:Carbohydrate Epimerases / Q000235:genetics; D002417:Cattle; D019453:Escherichia coli O157 / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000032:analysis; D008460:Meat / Q000382:microbiology; D019081:O Antigens / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods; D012703:Serotyping; D000637:Transaminases / Q000235:genetics
D008070:Lipopolysaccharides; D019081:O Antigens; D000637:Transaminases; D002238:Carbohydrate Epimerases; C061272:perosamine synthetase