Enhancement of XPG mRNA expression by human interferon-beta in Cockayne syndrome cells.
Using PCR-differential display, we have searched for genes expressed specially in human interferon (HuIFn)-beta-treated Cockayne syndrome (CS) fibroblast cells. Eighteen expressed genes induced by HuIFN-beta were identified, the sequences of seven of which were highly homologous to previously cloned sequences. The cDNAs of six of these seven clones were similar to expression tagged sequences from unknown genes in databases and the remaining one was identical to the cDNA of the xeroderma pigmentosum XPG gene. These results, together with our previous finding of increased resistance to ultraviolet (UV) cell-killing of CS cells pretreated with HuIFN-beta prior to UV irradiation suggest that XPG might be one of the genes possibly involved in the HuIFN-beta-induced UV-resistance.
Department of Pediatrics, Faculty of Medicine, University of Chiba, Japan. sugita@med.m.chiba-u.ac.jp
K
K
Sugita
N
N
Suzuki
Y
Y
Higuchi
K
K
Kita
Y
Y
Suzuki
A
A
Lehmann
D002478:Cells, Cultured; D002648:Child; D003001:Cloning, Molecular; D003057:Cockayne Syndrome / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D004720:Endonucleases; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011836:Radiation Tolerance; D017422:Sequence Analysis, DNA; D014157:Transcription Factors; D014466:Ultraviolet Rays; D015854:Up-Regulation; D000099250:DNA Excision Repair Protein ERCC-5
D004268:DNA-Binding Proteins; D004720:Endonucleases; D016899:Interferon-beta; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D000099250:DNA Excision Repair Protein ERCC-5