[Characterization of Haemophilus influenzae strains using sugar fermentation].
64 Haemophilus influenzae strains circulating in Havana City during a year were characterized by the carbohydrate fermentation method for the first time in Cuba. The fermentative pattern D was the most frequently found. Patterns D and G together were 72% of the total of strains studied. The combination of the carbohydrate fermentation with serotyping and biotyping allowed a greater differentiation of strains (14 groups). Patterns A, B, C and F appeared in children over 6 months of age, and pattern G in the group from 6 to 18. Patterns D and G predominated in the bacterial meningoencephalitis. A higher heterogeneity was observed among the strains isolated from acute respiratory infections. Some of the advantages of the Haemophilus influenzae strains subtyping method are stressed, such as: simplicity, easiness to be applied and interpreted, and the fact that it is not necessary a qualified personnel or a specialized laboratory for its implementation.
Instituto de Medicina Tropical Pedro Kourí.
R
R
Llanes Caballero
L E
LE
Azahares Romero
M F
MF
Pérez Monras
A
A
Martínez Díaz
D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D050260:Carbohydrate Metabolism; D002675:Child, Preschool; D005285:Fermentation; D006192:Haemophilus Infections / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007841:Latex Fixation Tests; D011446:Prospective Studies; D013045:Species Specificity
D001426:Bacterial Proteins