Kinesin accumulation in chick spinal axonal swellings with beta,beta'-iminodipropionitrile (IDPN) intoxication.
Kinesin is a major molecular motor responsible for anterograde axonal transport. Chicks were injected with beta,beta'-iminodipropionitrile (IDPN) to induce axonal swellings in spinal motor neurons and spinal sensory ganglion neurons. Cylindrical swollen axons were found in the anterior horn and anterior funiculus of the spinal cord, anterior root, and spinal ganglia. All of the axonal swellings were heavily stained with two anti-kinesin monoclonal antibodies. The swellings were mildly stained with an anti-cytoplasmic dynein and anti-tubulin antibodies, and weakly stained with an anti-tau antibody. These suggest the isolated disturbance of kinesin transport with neurofilament accumulation in IDPN intoxication.
Department of Internal Medicine, Akita University School of Medicine, Japan.
I
I
Toyoshima
K
K
Kato
M
M
Sugawara
C
C
Wada
O
O
Masamune
D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000473:pathology; D002645:Chickens; D007150:Immunohistochemistry; D016547:Kinesins / Q000187:drug effects / Q000378:metabolism*; D008868:Microtubule Proteins / Q000187:drug effects / Q000378:metabolism; D009046:Motor Neurons / Q000378:metabolism / Q000473:pathology; D009475:Neurons, Afferent / Q000378:metabolism / Q000473:pathology; D009498:Neurotoxins / Q000494:pharmacology; D009570:Nitriles / Q000494:pharmacology*; D013119:Spinal Cord Injuries / Q000139:chemically induced / Q000378:metabolism*
D008868:Microtubule Proteins; D009498:Neurotoxins; D009570:Nitriles; C008110:3,3'-iminodipropionitrile; D016547:Kinesins