Tissue-specific repression of starvation and stress responses of the Neurospora crassa con-10 gene is mediated by RCO1.
The Neurospora crassa con-10 gene is weakly expressed in mycelia but is induced approximately 1000-fold during macroconidiation. Studies of the promoter elements and trans-acting factors that regulate con-10 expression are needed to gain a detailed understanding of developmental regulation. The rco-1 mutant displays a 10-fold elevated basal level of expression of con-10. In contrast to the wild type, con-10 expression in mycelia of the rco-1 mutant was rapidly induced to high levels by starvation for carbon or nitrogen and by heat shock. Although con-10 is developmentally induced late in conidiation, con-10 was inducible by heat shock shortly after exposure of the wild-type mycelium to air. These findings support the view that RCO1 is a cell type-specific repressor of con-10. We propose that inactivation of RCO1 allows developing conidiophores to adjust the timing of con-10 induction in response to stress.
Lee
K
K
Department of Plant Pathology and Microbiology, Texas A&M University, College Station 77843-2132, USA.
Ebbole
D J
DJ
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002940:Circadian Rhythm; D005656:Fungal Proteins / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005947:Glucose / Q000494:pharmacology; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000235:genetics* / Q000254:growth & development; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D012097:Repressor Proteins; D013997:Time Factors
C113835:CON-10 protein, Neurospora crassa; D005656:Fungal Proteins; C102112:RCO-1 protein, Neurospora crassa; D012331:RNA, Fungal; D012333:RNA, Messenger; D012097:Repressor Proteins; D005947:Glucose