Vag8, a Bordetella pertussis bvg-regulated protein.
Bordetella pertussis expresses a bvg-regulated 95-kDa protein, Vag8, encoded by vag-8. Southern blot analysis indicates that strains of Bordetella bronchiseptica and Bordetella parapertussis have DNA homologous to vag-8. Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of B. pertussis and B. bronchiseptica but not B. parapertussis. A 95-kDa protein-negative derivative of B. pertussis 18323 containing a deletion of vag-8 colonized mice as efficiently as the parent B. pertussis strain in a mouse aerosol model of pertussis.
Finn
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Laboratory of Pertussis, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA. fin@cber.cber.fda.gov
Amsbaugh
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D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D001483:Base Sequence; D001884:Bordetella / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000502:physiology; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D016296:Mutagenesis; D010957:Plasmids; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D017421:Sequence Analysis; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000378:metabolism*
D001426:Bacterial Proteins; C094422:Bvg accessory factor protein, Bordetella pertussis; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D015534:Trans-Activators; D015500:Chloramphenicol O-Acetyltransferase; D000469:Alkaline Phosphatase