Biodegradation of poly(ethylene adipate) microcapsules in physiological media.
Spherical reservoir-type microcapsules fabricated using a water/oil/water (W/O/W) double emulsion technique with solvent evaporation and composed of poly(ethylene adipate) (PEAD) blended with 20% poly-epsilon-caprolactone (PCL II) containing a range of bovine serum albumin (BSA) loadings were incubated in Hank's buffer, pH 7.4, newborn calf serum, 1.5% pancreatin and synthetic gastric juice containing 10% pepsin A over 30 days and their percentage weight loss (PWL) and changes in ultrastructural morphology monitored by gravimetry and stereoscan electron microscopy (SEM) respectively. The greatest PWL from microcapsules was observed after incubation in newborn calf serum (NCS) and pancreatin and decreased in the order NCS > pancreatin > synthetic gastric juice > Hank's buffer. Only microcapsules theoretically loaded with 5-20% BSA and incubated in synthetic gastric juice showed a significant increase in PWL with increasing percentage BSA loading. The structural biodegradation of PEAD microcapsules in both Hank's buffer and synthetic gastric juice was minimal whilst the morphological changes observed during incubation in NCS involved pitting of the membrane, some surface erosion and reduction in diameter, followed by microcapsule membrane disruption and loss of reservoir contents. Biodegradation in pancreatin was associated with surface flaking and loss of large fragments of the microcapsule membrane. Only in NCS and pancreatin, where one would expect to see the effects of enzyme activity in addition to simple ester hydrolysis, did biodegradation proceed to the stage where there was a loss of spherical shape and almost total disruption of the microcapsule structure within 30 days.
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK. t.w.atkins@aston.ac.uk
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D000272:Adipates / Q000737:chemistry* / Q000493:pharmacokinetics*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000493:pharmacokinetics*; D001711:Biotransformation; D002214:Capsules; D002417:Cattle; D004655:Emulsions; D005750:Gastric Juice / Q000737:chemistry / Q000378:metabolism; D010194:Pancreatin / Q000737:chemistry / Q000378:metabolism; D010968:Plasticizers / Q000737:chemistry* / Q000493:pharmacokinetics*; D011095:Polyethylenes / Q000737:chemistry* / Q000493:pharmacokinetics*; D013552:Swine
D000272:Adipates; D001672:Biocompatible Materials; D002214:Capsules; D004655:Emulsions; D010968:Plasticizers; D011095:Polyethylenes; C029900:adipic acid; D010194:Pancreatin