Partial purification and characterization of RalF40I, a class II restriction endonuclease from Ruminococcus albus F-40, which recognizes and cleaves 5'-/GATC-3'.
Restriction endonuclease RalF40I was purified from cell-free extracts of the rumen cellulolytic bacterium Ruminococcus albus F-40 heparin-Sepharose chromatography. The preparation was active only on DNA substrates that were not Dammethylated. RalF401 recognizes the 4-bp palindrome, 5'-/GATC-3', and cleaves DNA at the 5' side of G in the sequence, producing 5' tetranucleotide protruding ends. RalF40I is a class II restriction endonuclease and an isoschizomer of MboI and DpnII.
School of Bioresources, Mie University, Tsu, Japan.
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Miyagi
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Javorský
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Pristas
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Karita
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Sakka
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Ohmiya
D000818:Animals; D002847:Chromatography, Agarose; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry* / Q000302:isolation & purification*; D010459:Peptococcaceae / Q000235:genetics* / Q000254:growth & development; D012417:Rumen / Q000382:microbiology
D015252:Deoxyribonucleases, Type II Site-Specific