Active efflux of norfloxacin by Bacteroides fragilis.
Norfloxacin was actively pumped out by Bacteroides fragilis, which is intrinsically resistant to most fluoroquinolones. Reserpine moderately inhibited the efflux. A one-step spontaneous mutant had increased resistance to norfloxacin, ethidium bromide, and puromycin, a result suggesting that the efflux is catalyzed by a multidrug pump with specificity similar to that of NorA/Bmr.
Miyamae
S
S
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya 464-8650, Japan.
Nikaido
H
H
Tanaka
Y
Y
Yoshimura
F
F
D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D001426:Bacterial Proteins / Q000502:physiology; D001441:Bacteroides fragilis / Q000187:drug effects / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D002352:Carrier Proteins / Q000502:physiology; D004352:Drug Resistance, Microbial; D026901:Membrane Transport Proteins; D008826:Microbial Sensitivity Tests; D009643:Norfloxacin / Q000493:pharmacokinetics* / Q000494:pharmacology
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D002352:Carrier Proteins; D026901:Membrane Transport Proteins; C075041:Bmr protein, Bacillus subtilis; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D009643:Norfloxacin