Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver.
Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum. These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial live support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module including primary hepatocyte spheroids was developed. The success of the system is indicated by an 80% recovery rate in hepatic failure rats which died in control experiments.
Ijima
H
H
Chemical Engineering Group. Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Nakazawa
K
K
Mizumoto
H
H
Matsushita
T
T
Funatsu
K
K
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002460:Cell Line; D002522:Chlorocebus aethiops; D004285:Dogs; D005112:Extracorporeal Circulation / Q000379:methods; D006801:Humans; D007668:Kidney / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D017093:Liver Failure / Q000628:therapy; D019164:Liver, Artificial; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D018874:Spheroids, Cellular; D013552:Swine; D014709:Vero Cells
D011140:Polyurethanes