Molecular cloning of rat Pax4: identification of four isoforms in rat insulinoma cells.
Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.
Second Department of Internal Medicine, Chiba University, School of Medicine, Japan.
Y
Y
Tokuyama
K
K
Yagui
K
K
Sakurai
N
N
Hashimoto
Y
Y
Saito
A
A
Kanatsuka
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics*; D007340:Insulinoma / Q000235:genetics*; D007515:Islets of Langerhans / Q000737:chemistry*; D008969:Molecular Sequence Data; D051761:Paired Box Transcription Factors; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018398:Homeodomain Proteins; D051761:Paired Box Transcription Factors; C491708:Pax4 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors