Determination of nifedipine in human plasma by flow-injection tandem mass spectrometry.
For use in clinical studies, a fast and sensitive assay method was developed for the determination of nifedipine in human plasma samples. The assay method is based on tandem mass spectrometry detection (HPLC-MS-MS). The effect of flow injection as well as HPLC separation on the results of the nifedipine determination were evaluated. The limit of quantification is 0.5 ng/ml and the accuracy (as determined by spiking recovery) was found to be good.
Dankers
J
J
BCO Analytical Services, Breda, The Netherlands.
van den Elshout
J
J
Ahr
G
G
Brendel
E
E
van der Heiden
C
C
D002121:Calcium Channel Blockers / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009543:Nifedipine / Q000097:blood*; D015203:Reproducibility of Results
D002121:Calcium Channel Blockers; D009543:Nifedipine