Physical assignment of two porcine microsatellites to the short arm of chromosome 1 by modified DISC-PCR.
Direct in situ single copy PCR (DISC-PCR) was modified to increase signal detection efficiency and physically assign two porcine microsatellites (MS), Sw552 and Sw137, to chromosome 1p at Flpter 0.1-0.2 and Flpter 0.01-0.2, respectively. These are the first two MS localized to chromosome 1p by an in situ method. The modifications of the DISC-PCR procedure included use of buffered formalin and pepsin as pre-reaction treatments, and tetramethylbenzidine (TMB) for signal detection. The detection efficiency of the modified method is twofold higher than that of the original technique.
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
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Troyer
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002874:Chromosome Mapping / Q000662:veterinary; D004076:Digoxigenin / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D008677:Metaphase; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000235:genetics*
D006241:Haptens; D001710:Biotin; D004076:Digoxigenin