Direct fractionation of proteins in particle-containing feedstocks by a filter paper pieces-based DEAE-cellulose column chromatography. Rapid, robust and low-cost capturing procedure for protein.
Filter paper pieces-based (FPB) DEAE-celluloses was prepared for direct fractionation of proteins in particle-containing feedstocks. FPB DEAE-cellulose has a protein binding capacity equivalent to that of commercially available DEAE-cellulose. Crude extracts from porcine intestine and kiwi fruit pulp, which were unmanageable by commercially available chromatographic media due to rapid clothing, could be directly fractionated with FPB DEAE-cellulose column. In addition, effluents from an FPB DEAE-cellulose column were extensively clarified. The present approach can be used as a rapid, robust and low-cost capturing step for protein from particle-containing feedstocks.
Sato
K
K
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Japan.
Guo
Y H
YH
Feng
J
J
Sugiyama
S
S
Ichinomiya
M
M
Tsukamasa
Y
Y
Minegishi
Y
Y
Sakata
A
A
Komiya
K
K
Yamasaki
Y
Y
Nakamura
Y
Y
Ohtsuki
K
K
Kawabata
M
M
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003365:Costs and Cost Analysis; D004044:Dietary Proteins / Q000032:analysis*; D005374:Filtration; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry; D008460:Meat / Q000032:analysis; D015203:Reproducibility of Results; D013552:Swine; D014783:Viscosity
D004044:Dietary Proteins