Expression of the 'dead box' RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetus.
The human DEAD box protein p68 is an established RNA-dependent ATPase and RNA helicase, p68 has been highly conserved in evolution and appears to be essential for normal growth, suggesting that this protein plays an important role in the cell. Although the biochemical activities of p68 are fairly well characterized, little is known about its biological function. This report shows that p68 is detectable in quiescent cell lines, but its expression is induced by serum, suggesting that this protein may play a role in cell growth. It is also shown that both p68 mRNA and protein are differentially expressed in adult tissues; in this case, however, the levels do not always correlate with proliferation status, suggesting that the regulation of expression in the animal may be different from that in cell lines. Finally, it is shown that p68 expression is developmentally regulated and appears to correlate with organ differentiation/maturation. These findings suggest that p68 expression may not simply reflect proliferation/differentiation status and that it appears to be regulated in a more complex way.
Department of Molecular and Cellular Pathology, University of Dundee, Ninewells Medical School, U.K.
R J
RJ
Stevenson
S J
SJ
Hamilton
D E
DE
MacCallum
P A
PA
Hall
F V
FV
Fuller-Pace
D000818:Animals; D001769:Blood; D015152:Blotting, Northern; D015153:Blotting, Western; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D053487:DEAD-box RNA Helicases; D005314:Embryonic and Fetal Development / Q000235:genetics / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007124:Immunoenzyme Techniques; D007438:Introns; D051379:Mice; D008815:Mice, Inbred Strains; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D009713:Nucleotidyltransferases / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011494:Protein Kinases; D020365:RNA Helicases; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats
D009687:Nuclear Proteins; D012333:RNA, Messenger; D011494:Protein Kinases; D009713:Nucleotidyltransferases; C506749:Ddx5 protein, human; C506751:Ddx5 protein, mouse; D053487:DEAD-box RNA Helicases; C506752:Ddx5 protein, rat; D020365:RNA Helicases