Inhibition of the system luminol-H2O2-Fe(CN)6(3)-chemiluminescence by the Mn(II) indirect determination of isoniazid in a pharmaceutical formulation.
A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate (III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation.
Gregorio Alapont
A
A
Departamento de Química, Colegio Universitario CEU San Pablo, Moncada, Valencia, Spain.
Aurechia Giménez
E
E
Lahuerta Zamora
L
L
Martínez Calatayud
J
J
D002021:Buffers; D002626:Chemistry, Pharmaceutical; D005292:Ferricyanides; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007538:Isoniazid / Q000032:analysis*; D008163:Luminescent Measurements; D008165:Luminol; D017895:Manganese Compounds; D010087:Oxides; D013464:Sulfuric Acids
D002021:Buffers; D005292:Ferricyanides; D007202:Indicators and Reagents; D017895:Manganese Compounds; D010087:Oxides; D013464:Sulfuric Acids; C007931:hexacyanoferrate III; D008165:Luminol; D006861:Hydrogen Peroxide; C033158:sulfuric acid; C016552:manganese dioxide; D007538:Isoniazid