Characterization and discrimination of three Theileria parva stabilates involved in East Coast fever vaccination.
Three vaccine stabilates of Theileria parva, of which sporozoites are being used against East Coast fever, were characterized by immunological and molecular biology techniques before being used for a national vaccination campaign in Kenya. T. parva Marikebuni stabilates 316 and 3014, and T. parva Lanet were used in this study and were discriminated from other Kenyan field Theileria isolates. IFAT results showed that all the animals were producing antibodies regardless of the stock used. Primers designed on the TPR1 gene sequence were used for PCR and Decamers were used for RAPD. Specific DNA band patterns (1,877 bp; 1,059 bp, and 443 bp) for the three vaccine stocks were observed. These molecular markers could be used to trace vaccinated animals in Kenya and to identify which isolates are responsible for reactions in animals.
Centre for Tropical Veterinary Medicine, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Scotland, UK. olivier.sparagano@ed.ac.uk
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Sparagano
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Zanaa
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Ambrose
D000818:Animals; D002417:Cattle; D016000:Cluster Analysis; D017931:DNA Primers; D014644:Genetic Variation; D007630:Kenya; D010802:Phylogeny; D016052:Protozoan Vaccines; D019105:Random Amplified Polymorphic DNA Technique; D016797:Theileria parva / Q000145:classification* / Q000235:genetics / Q000276:immunology*; D013801:Theileriasis / Q000276:immunology* / Q000517:prevention & control
D017931:DNA Primers; D016052:Protozoan Vaccines