Decreased DTH response to recall antigens in mice injected with human immunodeficiency virus type 1-infected U937 cells and infected with Candida albicans.
In previous studies, we have reported that the intraperitoneal (i.p.) injection of HIV-1 infected human U937 cells into normal mice resulted in long-term persistence of anti-HIV antibodies and in a small percentage (10-20%) of HIV-1 infected animals at 6-12 months after the injection. The study reported here was undertaken to detect T immune defects in U937-HIV-1-injected mice. Eight months after the initial injection, a marked decrease in DTH response against U937 cells was detected in HIV injected animals. In addition, a consistent decrease in DTH response against a soluble mannoprotein antigen of Candida albicans cell wall (MP-F2) was also observed in U937-HIV-1-injected mice, chronically infected with low-virulent strain of the fungus. No decreases in DTH response was observed in control-injected animals. These data indicate that U937-HIV-1-injected mice become unable to mount a normal antigen-specific immune response. Although the mechanisms involved in the generation of these T cell defects remain unclear, these events appear to be somehow related to the HIV-1 infection and should be considered in the current studies of HIV-1 infection with transgenic mice.
Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, Roma, Italy.
R
R
Lande
P
P
Sestili
M
M
Spada
A
A
Cassone
F
F
Belardelli
C M
CM
Ausiello
D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000276:immunology*; D017635:Clonal Anergy; D004195:Disease Models, Animal; D005656:Fungal Proteins / Q000494:pharmacology; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000821:virology; D008223:Lymphoma / Q000821:virology; D008297:Male; D008562:Membrane Glycoproteins / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation
D005656:Fungal Proteins; D008562:Membrane Glycoproteins; C025363:mannoproteins