A sandwich enzyme immunoassay for measuring AD7C-NTP as an Alzheimer's disease marker: AD7C test.
A simple, fast, reliable, and specific immunoassay has been developed to detect and measure AD7C-NTP, a biochemical marker for Alzheimer's disease, in cerebrospinal fluid (CSF). This assay, called the AD7C Test, is an enzyme-linked sandwich immunoassay (ELSIA) using 96 well microtiter plates. The plate surface is coated with a monoclonal antibody (N3I4) which has a high affinity and specificity for AD7C-NTP, capturing it effectively from CSF samples. The detection was achieved using a polyclonal antibody (ADRI). Both N3I4 and ADRI were generated using recombinantly produced AD7C-NTP. The assay is highly sensitive (30-50 pg), linear to 2.0 ng (r2 > 0.99), and reproducible (C.V. < 10%). The utility of the assay has been demonstrated using CSF specimens from early Alzheimer's disease patients and age matched controls (sensitivity of 89% and specificity of 89%).
Ghanbari
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Nymox Corporation, Rockville, MD 20895, USA.
Ghanbari
H A
HA
D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D015415:Biomarkers / Q000134:cerebrospinal fluid; D006801:Humans; D007124:Immunoenzyme Techniques; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid* / Q000276:immunology; D011994:Recombinant Proteins / Q000134:cerebrospinal fluid / Q000276:immunology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
C109804:AD7c-NTP; D000906:Antibodies; D000911:Antibodies, Monoclonal; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins