[Evaluation of a western blot system (DAVIH-BLOT) for the confirmation of HIV-1 antibodies].
It is reported the evaluation of the DAVIH-BLOT system (DAVIH Laboratories, Cuba) against the proficiency panels sent to the National AIDS Reference Laboratory by the World Health Organization (WHO) in order to ensure the quality in the diagnosis of HIV antibodies during 1989, 1991 and 1993. The system is also compared with those of the Dupont and Diagnostic Pasteur firms in a group of 467 positive sera by ELISA. On studying the WHO's samples, the system proved to be highly sensitive and specific. In the comparison made with the HIV-1 Western Blot IgG version 1.2 (Dupont company, USA) and with the LAV-blot 1 (Diagnostic Pasteur, France), there was a coincidence of 100 and 97%, respectively.
Centro de Investigaciones Científicas, Defensa Civil Laboratorio de Investigaciones del Sida.
O
O
Cruz Sui
M T
MT
Pérez Guevara
M
M
Izquierdo Márquez
L
L
Lobaina Batelemy
I
I
Ruibal Brunet
E
E
Silva Cabrera
D015492:AIDS Serodiagnosis / Q000379:methods*; D015153:Blotting, Western / Q000379:methods* / Q000592:standards; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D015483:HIV Antibodies / Q000097:blood*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D011933:Reagent Kits, Diagnostic