Seasonal allergic conjunctivitis is accompanied by increased mast cell numbers in the absence of leucocyte infiltration.
BACKGROUND
Seasonal allergic conjunctivitis (SAC) is the most common allergic disease to affect the eye, occurring alone or in association with allergic rhinitis. Infiltration with mast cells and eosinophils is characteristic of the chronic forms of allergic conjunctivitis such as vernal and atopic keratoconjunctivitis, and these cell types also contribute significantly to allergic inflammation in the skin. Indirect evidence for a similar pattern of cellular events in SAC comes from studies which demonstrate raised eosinophil and neutrophil numbers in conjunctival scrapings and elevated levels of mast cell tryptase in tears following allergen challenge.


OBJECTIVE
To directly characterize the inflammatory cell infiltrate in SAC and to determine its clinical relevance.


METHODS
We employed specific immunohistochemical staining to count mast cells, eosinophils and neutrophils in the conjunctival epithelium and lamina propria of eight atopic patients with SAC in, and 12 SAC patients out of the hay fever season. Sixteen patients with no history of ocular allergy were used as control subjects.


RESULTS
Mast cells were absent from normal epithelium. During the pollen season median mast cell numbers in the lamina propria were found to be increased by 61% in patients with SAC compared with normals (P=0.012). Eosinophils were found in the lamina propria in less than half of the symptomatic patients with SAC and in only three patients were eosinophils present in the epithelium. The neutrophil numbers in the lamina propria of patients with SAC tended to be higher than normals but these changes did not reach statistical significance.


CONCLUSIONS
These data based on the direct assessment of conjunctival tissue provide evidence that symptoms occur in SAC in the absence of detectable recruitment of eosinophils or neutrophils. This suggests that this disorder is related to mast cell-mediated changes.
University Pathology, University of Southampton, Southampton General Hospital, UK.
D F
DF
Anderson
J D
JD
MacLeod
S M
SM
Baddeley
A S
AS
Bacon
J I
JI
McGill
S T
ST
Holgate
W R
WR
Roche
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D003228:Conjunctiva / Q000166:cytology / Q000276:immunology; D003233:Conjunctivitis, Allergic / Q000276:immunology* / Q000473:pathology; D004804:Eosinophils / Q000276:immunology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D008875:Middle Aged; D009504:Neutrophils / Q000276:immunology; D012621:Seasons; D012882:Skin Tests