Molecular cloning of rat Pax4: identification of four isoforms in rat insulinoma cells.
Molecular cloning of rat Pax4 cDNA from a rat insulinoma cell line, RINm5F, library by PCR-based cloning strategy revealed four isoforms of the protein. Analysis of tissue distribution using Northern blotting and RT-PCR showed specific expression of Pax4 mRNA in pancreatic islets and RIN cells. RT-PCR confirmed that the mRNAs of four isoforms are expressed in RIN cells. These Pax4 variants may regulate the transcriptional activity of Pax4 during the development of pancreatic islets.
Tokuyama
Y
Y
Second Department of Internal Medicine, Chiba University, School of Medicine, Japan.
Yagui
K
K
Sakurai
K
K
Hashimoto
N
N
Saito
Y
Y
Kanatsuka
A
A
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000235:genetics*; D007340:Insulinoma / Q000235:genetics*; D007515:Islets of Langerhans / Q000737:chemistry*; D008969:Molecular Sequence Data; D051761:Paired Box Transcription Factors; D010190:Pancreatic Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D051381:Rats; D014157:Transcription Factors / Q000737:chemistry* / Q000235:genetics*; D014407:Tumor Cells, Cultured
D018398:Homeodomain Proteins; D051761:Paired Box Transcription Factors; C491708:Pax4 protein, rat; D012333:RNA, Messenger; D014157:Transcription Factors