Release of lubricating synovial surfactant by intra-articular steroid.
This study was undertaken to determine whether glucocorticosteroids promote the secretion of lubricating surfactant, i.e. surface-active phospholipid (SAPL), into the joint. A standard clinical dose (100 mg) of methylprednisolone acetate (MPA) in 2.5 ml of saline was injected into the load-bearing right radiocarpal joint of five horses and 2.5 ml of saline injected into each of the contralateral joints used as controls. Synovial fluid (SF) was aspirated from all 10 joints before injection and at intervals of 16 and 32 h after injection, and then analysed by standard methods. All test joints showed an elevated level of SAPL, the increases averaging 112% after 16 h and 76% after 32 h, which were highly significant relative to the control joints. A large increase at 16 h was also found in proteolipid as a possible further marker of surfactant release. Significant quantities of proteolipid were also found in human SF. Since intra-articular steroids can dramatically improve joint mobility in both humans and horses, it is proposed that part of the benefit may be derived from improved lubrication arising from the remarkable ability of SAPL to lubricate under high load. Other possible benefits of elevating surfactant levels in the joints include control of cartilage hydration, promotion of macrophage activity and the ability to scavenge oxygen free radicals.
Paediatric Respiratory Research Centre, Mater Children's Hospital, Brisbane, Queensland, Australia.
B A
BA
Hills
M T
MT
Ethell
D R
DR
Hodgson
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000378:metabolism*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002350:Carpus, Animal / Q000187:drug effects* / Q000378:metabolism; D006736:Horses / Q000502:physiology*; D007270:Injections, Intra-Articular; D008775:Methylprednisolone / Q000031:analogs & derivatives* / Q000494:pharmacology; D000077555:Methylprednisolone Acetate; D013582:Synovial Fluid / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D015060:1,2-Dipalmitoylphosphatidylcholine; D000077555:Methylprednisolone Acetate; D008775:Methylprednisolone