Mitochondria and cells produce reactive oxygen species in virtual anaerobiosis: relevance to ceramide-induced apoptosis.
Observations of apoptosis in virtual anaerobiosis have raised doubts on the significance of reactive oxygen species in the cascade of events of programmed cell death. This work presents evidence that cells and mitochondrial preparations produce similar levels of hydrogen peroxide under either aerobic or virtually anaerobic conditions. These levels are relevant to the increased production of radicals induced by a ceramide analog that promotes apoptosis. This ceramide acts at center o of mitochondrial complex III.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia. mauro.esposti@med.monash.edu.au
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Degli Esposti
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McLennan
D000693:Anaerobiosis; D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D017209:Apoptosis / Q000502:physiology*; D019556:COS Cells / Q000378:metabolism*; D002417:Cattle; D002518:Ceramides / Q000494:pharmacology*; D014450:Electron Transport Complex III / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D008929:Mitochondria, Heart / Q000737:chemistry / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013267:Stilbenes / Q000494:pharmacology; D013367:Submitochondrial Particles / Q000737:chemistry / Q000378:metabolism*
D002518:Ceramides; D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D013267:Stilbenes; C101954:N-caproylsphingosine; C056108:3-methoxy-2-(2-styrylphenyl)propenic acid methyl ester; C032456:antimycin; D000968:Antimycin A; D006861:Hydrogen Peroxide; D014450:Electron Transport Complex III