Identification of D-aspartate in rat pheochromocytoma PC12 cells.
D-Aspartate is now known to be present in mammalian neuronal and endocrine cells in vivo, and may play some role(s) in neurocrine and endocrine functions. However, origin of D-aspartate is unknown. Here, we report that free D-aspartate (108 pmoles/3 x 10(7) cells) is present in the cultured PC12 cells, a rat pheochromocytoma cell line, as determined with immunohistochemical techniques as well as high performance liquid chromatography (HPLC) on a Pirkle-type chiral column. The amount of D-aspartate does not change with the passage. The culture medium does not contain D-aspartate. These results strongly suggest the presence of a de novo biosynthetic pathway for D-aspartate in the endocrine cells.
Moriyama
Y
Y
Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Japan. moriyama@sanken.osaka-u.ac.jp
Yamada
H
H
Hayashi
M
M
Oda
T
T
Yamaguchi
A
A
D000818:Animals; D000906:Antibodies; D001224:Aspartic Acid / Q000032:analysis* / Q000276:immunology; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D007536:Isomerism; D016716:PC12 Cells / Q000737:chemistry*; D051381:Rats
D000906:Antibodies; D004396:Coloring Agents; D001224:Aspartic Acid