The morphology of the polyhedra of a host range-expanded recombinant baculovirus and its parents.
The host range-expanded recombinant baculovirus, RecB-8 was isolated from BmN-4 cells coinfected with Autographa californica and Bombyx mori nuclear polyhedrosis viruses. Its genome was compared with those of its parents by restriction endonuclease digestion and their polyhedra compared in an electron microscope. Interestingly, the polyhedra of RecB-8 were tetrahedral although the polyhedrin gene was the same as that of the BmNPV parent which has icosahedral polyhedra. Thus the morphology of the RecB-8 polyhedra resulted from host cell factors and/or another viral genome in the host cells.
Woo
S D
SD
Department of Agricultural Biology, College of Agriculture & Life Sciences, Seoul National University, Suwon, Korea.
Kim
W J
WJ
Kim
H S
HS
Jin
B R
BR
Lee
Y H
YH
Kang
S K
SK
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000648:ultrastructure*; D000077183:Occlusion Body Matrix Proteins; D011995:Recombination, Genetic; D018411:Spodoptera; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus