Individual promoter and intron p53-binding motifs from the rat Cyclin G1 promoter region support transcriptional activation by p53 but do not show co-operative activation.
The rat Cyclin G1 gene promoter contains one p53-binding motif upstream of the transcription start site, and a second motif downstream in the first intron. We have investigated the possibility that these motifs co-operate to permit high level promoter activation by p53. Although individual motifs supported p53-dependent, orientation-independent transcriptional activation, using reporter plasmids containing both motifs, we found no evidence for co-operative promoter activation either after co-transfection with human p53 expression plasmids, or after exposure of transfected cells to cisplatin and UV-radiation.
Oncology Research Centre, Prince of Wales Hospital, Randwick, NSW, Australia.
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Yardley
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Zauberman
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Oren
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Jackson
D000818:Animals; D001665:Binding Sites; D002945:Cisplatin / Q000494:pharmacology; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins / Q000235:genetics*; D004249:DNA Damage; D016158:Genes, p53; D006801:Humans; D007438:Introns / Q000235:genetics*; D007739:L Cells; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D051381:Rats; D015533:Transcriptional Activation / Q000235:genetics*; D014162:Transfection; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D014466:Ultraviolet Rays; D001749:Urinary Bladder Neoplasms
C534702:CCNG1 protein, human; C534703:Ccng1 protein, mouse; C534704:Ccng1 protein, rat; D056746:Cyclin G; D056766:Cyclin G1; D016213:Cyclins; D016159:Tumor Suppressor Protein p53; D002945:Cisplatin