Reactivities of feline calicivirus field isolates with monoclonal antibodies detected by enzyme-linked immunosorbent assay.
Reactivities of feline calicivirus (FCV) field isolates with monoclonal antibodies (MAbs) were examined by enzyme-linked immunosorbent assay (ELISA). The reactivities of the viruses in ELISA were different from our previous results using the neutralization tests (NT). Many isolates were positive in ELISA with MAbs which recognized neutralizing epitope 3B and/or 4. However, most were negative in NT in our previous study. After absorption of two FCV strains with host cells, the non-infectious virus fluid still reacted with MAb, which recognized epitope 3B and/or 4 in ELISA. These results indicated the possibility that neutralizing epitopes are expressed on non-infectious virus particles or exist as proteinaceous molecules in virus fluid.
Tajima
T
T
Department of Veterinary Microbiology, College of Agriculture, Osaka Prefecture University, Japan.
Takeda
Y
Y
Tohya
Y
Y
Sugii
S
S
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology; D017927:Calicivirus, Feline / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification*; D002415:Cats; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000276:immunology; D007074:Immunoglobulin G; D051379:Mice; D009500:Neutralization Tests
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D007074:Immunoglobulin G