HLA-A towards a high-resolution DNA typing.
Sequencing-based typing (SBT) and sequence-specific oligonucleotide probing (PCR-SSOP) are DNA-based typing approaches to identify HLA-A alleles. In this study PCR-SSOP SBT have been evaluated and considered to reach a high-resolution typing. Based upon serological typing, 32 genomic samples were typed by SBT and PCR-SSOP Three main clusters of resolution could be defined. The advantage of the PCR-SSOP approach is the possibility to type numerous samples in a short time. SBT minimizes the number of ambiguous heterozygous combinations and often allows direct detection and identification of new alleles.
Scheltinga
S A
SA
University Hospital Utrecht, Department of Pathology, The Netherlands.
Williams
F
F
van der Zwan
A W
AW
Rozemuller
E H
EH
Middleton
D
D
Tilanus
M G
MG
D004247:DNA; D015234:HLA-A Antigens / Q000145:classification* / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D015234:HLA-A Antigens; D004247:DNA