A mammalian two pore domain mechano-gated S-like K+ channel.
Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are closed by serotonin via cAMP-dependent phosphorylation, whereas they are opened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ channel sharing the properties of the S channel. In addition, the recombinant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a charged region critical for chemical and mechanical activation, as well as a phosphorylation site required for cAMP inhibition.
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Valbonne, France.
A J
AJ
Patel
E
E
Honoré
F
F
Maingret
F
F
Lesage
M
M
Fink
F
F
Duprat
M
M
Lazdunski
D000595:Amino Acid Sequence; D000818:Animals; D001048:Aplysia; D016718:Arachidonic Acid / Q000494:pharmacology; D019556:COS Cells; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D006801:Humans; D007447:Invertebrate Hormones / Q000737:chemistry / Q000502:physiology; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D015221:Potassium Channels / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D024683:Potassium Channels, Tandem Pore Domain; D017434:Protein Structure, Tertiary; D051669:Shab Potassium Channels
D007447:Invertebrate Hormones; C109137:KCNK1 protein, human; D015221:Potassium Channels; D024683:Potassium Channels, Tandem Pore Domain; D051669:Shab Potassium Channels; C085558:Shab protein, Aplysia; C103742:potassium channel protein TREK-1; D016718:Arachidonic Acid; D017868:Cyclic AMP-Dependent Protein Kinases