Comparison of C18-carboxypropylbetaine and glass bead DNA extraction methods for detection of Mycobacterium bovis in bovine milk samples and analysis of samples by PCR.
The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.
Department of Biology, Colorado State University, Fort Collins, Colorado 80523, USA.
B J
BJ
Cornejo
A
A
Sahagún-Ruiz
F
F
Suárez-Güemes
C G
CG
Thornton
T A
TA
Ficht
L G
LG
Adams
D000818:Animals; D001431:Bacteriological Techniques; D001622:Betaine / Q000031:analogs & derivatives; D002417:Cattle; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005898:Glass; D008863:Microspheres; D008892:Milk / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D014374:Tuberculin Test / Q000662:veterinary; D014380:Tuberculosis, Bovine / Q000175:diagnosis*
D004269:DNA, Bacterial; D001622:Betaine