Acetyl phosphate and the phosphorylation of OmpR are involved in the regulation of the cell division rate in Escherichia coli.
Carbon sources that can be converted to acetate were added to the growth medium of Escherichia coli wild-type cells. Cells responded with an increased cell division rate. The addition of acetate also caused a decreased synthesis of flagella. Mutants in phosphotransacetylase, which are incapable of synthesizing acetyl phosphate, and mutants in the osmoregulator OmpR divided at a lower rate than did wild-type cells. The mutants did not increase their cell division rate upon the addition of serine, as observed for wild-type cells. These data are consistent with the idea that the previously described effect of serine upon the cell division rate is mediated by acetyl phosphate and phosphorylation of OmpR.
Department of Microbiology and Immunology, University of Illinois at Chicago, IL 60612-7344, USA. birgit.pruess@lrz.tu-muenchen.de
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Prüss
D000085:Acetates / Q000494:pharmacology; D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D004926:Escherichia coli / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D005407:Flagella / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation; D010755:Organophosphates / Q000378:metabolism*; D010709:Phosphate Acetyltransferase / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D012694:Serine / Q000378:metabolism; D015398:Signal Transduction; D013912:Threonine / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
D000085:Acetates; D001426:Bacterial Proteins; D003470:Culture Media; D010755:Organophosphates; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; D013912:Threonine; D001224:Aspartic Acid; D018698:Glutamic Acid; D012694:Serine; C011632:acetyl phosphate; D002244:Carbon; D010709:Phosphate Acetyltransferase