Teichoic acid content in different lineages of Staphylococcus aureus NCTC8325.
A series of mec transformants of Staphylococcus aureus strain NCTC8325 were analysed for alterations in wall teichoic acid and lipoteichoic acid. Although the methicillin resistance determinant alters the autolytic behaviour of S. aureus, it had no effects on the cellular content, chain length, and alanine substitution of the lipoteichoic acid, or on the wall teichoic acid content and composition. However, independently of the presence or absence of the methicillin resistance determinant, level of methicillin resistance, or autolytic behaviour, a correlation was found between a 25% reduced cell wall phosphate content and either loss of prophages phi11 and 13 or a 30-kb deletion in the chomosmal SmaI-F fragment adjacent to the prophage &phi;11 attachment site.
Institute of Medical Microbiology, University of Zürich, Gloriastrasse 32, Postfach, CH-8028 Zürich, Switzerland.
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Berger-Bächi
D000117:Acetylglucosamine / Q000032:analysis; D000409:Alanine / Q000032:analysis; D001287:Attachment Sites, Microbiological / Q000235:genetics; D001433:Bacteriolysis; D002473:Cell Wall / Q000737:chemistry* / Q000235:genetics; D002872:Chromosome Deletion; D015252:Deoxyribonucleases, Type II Site-Specific; D005990:Glycerol / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis* / Q000737:chemistry; D008242:Lysogeny; D016106:Methicillin Resistance / Q000235:genetics; D010710:Phosphates / Q000032:analysis; D012255:Ribitol / Q000032:analysis; D013204:Staphylococcus Phages / Q000378:metabolism; D013211:Staphylococcus aureus / Q000737:chemistry* / Q000187:drug effects; D013682:Teichoic Acids / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
D008070:Lipopolysaccharides; D010710:Phosphates; D013682:Teichoic Acids; D012255:Ribitol; C009900:lipoteichoic acid; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D000409:Alanine; D005990:Glycerol; D000117:Acetylglucosamine