Basal forebrain neurons adjacent to the globus pallidus co-express GABAergic and cholinergic marker mRNAs.
Semi-quantitative single cell RT-PCR techniques were used to determine the expression of mRNAs related to GABAergic and cholinergic neurotransmission in neurons of the rat globus pallidus and adjacent basal forebrain region. Neurons of the globus pallidus expressed relatively high levels of GAD67 and GABA vesicular transporter mRNA but undetectable levels of ChAT or ACh vesicular transporter mRNA. In contrast, nominally basal forebrain neurons co-expressed ChAT and GAD67 mRNAs and mRNAs for both ACh and GABA vesicular transporters. These results suggest that the neurons along the medial border of the globus pallidus may co-release GABA and ACh.
Department of Anatomy and Neurobiology, College of Medicine, University of Tennessee, Memphis 38163, USA.
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Tkatch
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Baranauskas
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Surmeier
D000818:Animals; D015415:Biomarkers; D002352:Carrier Proteins / Q000096:biosynthesis; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D050485:GABA Plasma Membrane Transport Proteins; D005917:Globus Pallidus / Q000166:cytology / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D026901:Membrane Transport Proteins; D009474:Neurons / Q000378:metabolism*; D027361:Organic Anion Transporters; D010275:Parasympathetic Nervous System / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016548:Prosencephalon / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D015415:Biomarkers; D002352:Carrier Proteins; D050485:GABA Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D012333:RNA, Messenger; C493461:Slc18a3 protein, rat; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D005680:gamma-Aminobutyric Acid; D004247:DNA; D002795:Choline O-Acetyltransferase; D005968:Glutamate Decarboxylase