Monoclonal antibodies against human endothelin-converting enzyme-1.
Endothelin-converting enzyme-1 (ECE-1) is a membrane-bound metalloprotease which specifically converts the inactive precursor big-endothelin-1 (big ET-1) to the vasoactive endothelin-1 (ET-1). Six different mouse hybridoma cell lines have been generated secreting monoclonal antibodies specific to human ECE-1. These antibodies have been proven useful in a fast and efficient one-step purification of membrane-bound ECE-1 as well as of artificial soluble ECE-1 by immunoaffinity chromatography. The antibodies are suitable for a quantification of ECE-1 in solution by a sandwich-ELISA and for the immunohistochemical detection of ECE-1 in the cell membrane.
Main Laboratory, BASF Aktiengesellschaft, Ludwigshafen, Germany.
T
T
Subkowski
H
H
Hillen
B
B
Kröger
M
M
Schmidt
D000818:Animals; D000911:Antibodies, Monoclonal; D016282:Aspartic Acid Endopeptidases / Q000276:immunology* / Q000302:isolation & purification; D002462:Cell Membrane / Q000737:chemistry; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D018076:DNA, Complementary; D000072396:Endothelin-Converting Enzymes; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas; D015151:Immunoblotting; D007150:Immunohistochemistry; D008666:Metalloendopeptidases; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D015203:Reproducibility of Results
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; C000606925:Ece1 protein, mouse; D000072396:Endothelin-Converting Enzymes