Effect of polyethylene particles on human osteoblastic cell growth.
In this study, we have analyzed the direct effect of ultrahigh molecular weight polyethylene (polyethylene) on the osteoblastic cell growth in primary cultures. The cells were cultured from human bone samples obtained during reconstructive joint surgery. When cell cultures reached confluence (4-6 weeks) they were separated into three subcultures. One subculture was without particle addition (plain culture). In the other two subcultures, polyethylene or alumina was added. Two different sizes of particles were used, <80 and <160 microm. The subcultures were incubated until confluence. Proliferation of each subculture was measured by cell counts after 3, 6, 9 and 13 days, and the area under the curve (AUC) was calculated. Polyethylene particles of <160 microm induced a decrease in growth, whereas alumina of the same size did not. Polyethylene and alumina particles of <80 microm induced an inhibition in the osteoblastic cell growth; <80 microm polyethylene induced a higher inhibition than alumina of the same particle size. In conclusion, we have observed a direct effect of polyethylene on osteoblastic cell growth. This study shows that polyethylene may decrease the growth rate of human osteoblastic cells in primary cultures. Smaller particles produce a more marked reduction.
Biochemistry Division, Servicio de Bioquímica Clinica, Hospital La Paz, Madrid, Spain. emartinez@hulp.es
M E
ME
Martínez
S
S
Medina
M T
MT
del Campo
J A
JA
García
A
A
Rodrigo
L
L
Munuera
D001672:Biocompatible Materials / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*; D010316:Particle Size; D011095:Polyethylenes / Q000494:pharmacology*
D001672:Biocompatible Materials; D011095:Polyethylenes