Adenylyl cyclase messenger ribonucleic acid in myometrium: splice variant of type IV.
Regulation of uterine tone during pregnancy and parturition involves G protein-coupled receptors that modulate cellular levels of cAMP. Cyclic AMP is synthesized by a family of at least nine adenylyl cyclase enzymes that have unique tissue distributions, basal activities, and modes of regulation. Little is known regarding the expression of adenylyl cyclase isoforms in myometrium. Reverse transcription-polymerase chain reaction (PCR) was performed using RNA extracted from freshly dissected rat uteri and a cell line of homogeneous rat myometrial cells. Using PCR primers specific for adenylyl cyclases I-IX, mRNA encoding adenylyl cyclases II-IX were identified in both fresh and cultured myometrial cells. A splice variant of adenylyl cyclase type IV was also identified in RNA from both sources. These data show that multiple isoforms of adenylyl cyclases are expressed at the mRNA level in myometrial cells and suggest that the regulation of cellular levels of cAMP likely involves a complex integration of cellular second messengers acting upon multiple isoforms of adenylyl cyclases.
Department of Anesthesiology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA. cemala@welchlink.welch.jhu.edu
C W
CW
Emala
D
D
Kumasaka
C A
CA
Hirshman
K S
KS
Lindeman
D000262:Adenylyl Cyclases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D014644:Genetic Variation; D015151:Immunoblotting; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D009215:Myometrium / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017207:Rats, Sprague-Dawley
D007527:Isoenzymes; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D000262:Adenylyl Cyclases