The antioxidant action of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyra z in-3-one (MCLA), a chemiluminescence probe to detect superoxide anions.
The antioxidant effect of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O2.-, was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2'-azobis (2-amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. MCLA profoundly inhibited the propagation reaction in Fe2+-dependent lipid peroxidation in liposomes, and MCLA disappearance accompanied by suppression of oxygen consumption markedly occurred in liposomes susceptible to peroxidation. Thiobarbituric acid-reactive substances in all systems used were also suppressed by MCLA dose dependently. These results indicate that MCLA has an antioxidant property through scavenging free radicals.
Tampo
Y
Y
Division of Environmental Hygiene, Hokkaido College of Pharmacy, Japan. Tampo@hit.ac.jp
Tsukamoto
M
M
Yonaha
M
M
D000578:Amidines; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives; D011756:Diphosphates; D016166:Free Radical Scavengers; D007093:Imidazoles / Q000494:pharmacology*; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D008163:Luminescent Measurements; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D015335:Molecular Probes; D010101:Oxygen Consumption; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances
D000578:Amidines; D000975:Antioxidants; D001555:Benzene Derivatives; D011756:Diphosphates; D016166:Free Radical Scavengers; D007093:Imidazoles; D008081:Liposomes; D015335:Molecular Probes; D011719:Pyrazines; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; C046728:2,2'-azobis(2-amidinopropane); D007501:Iron; C007164:cumene hydroperoxide; C049051:ferric pyrophosphate