A reproducible, simple, and sensitive high-performance capillary electrophoresis method for simultaneous determination of capreomycin, ofloxacin and pasiniazide in urine.
Separation and determination of capreomycin (Cp), ofloxacin (Oflx) and pasiniazide (Ipa) in urine by high-performance capillary electrophoresis (HPCE) with 280 nm detection have been studied systematically. The calibration lines were linear in the range of 0.5 approximately 50 mg 1(-1), and the detection limits (S/N = 3) were 0.15, 0.20 and 0.10 mg 1(-1) for Cp, Oflx and Ipa, respectively. The recoveries for these materials from urine were higher than 93.5%. The accuracy and intra- and inter- day reproducibility of Cp, Oflx and Ipa were determined with satisfactory results. This method was successfully used for determining Cp. Oflx and Ipa in human urine.
Zhang
S S
SS
Center of Instrumental Analysis, Zhengzhou University, P.R. China.
Liu
H X
HX
Yuan
Z B
ZB
Yu
C L
CL
D010131:Aminosalicylic Acid / Q000652:urine; D000636:Aminosalicylic Acids; D002207:Capreomycin / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007538:Isoniazid / Q000031:analogs & derivatives* / Q000652:urine; D015242:Ofloxacin / Q000652:urine*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000636:Aminosalicylic Acids; D002207:Capreomycin; D010131:Aminosalicylic Acid; C008767:pasiniazide; D015242:Ofloxacin; D007538:Isoniazid