Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state.
The single stranded [3H]oligonucleotide uptake by HL-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20-45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6-13x) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10-15x). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11x) by DUSF if the cells were subjected to blast transformation.
Department of Biology, University Medical School of Debrecen, Hungary.
G
G
Tóth
J
J
Schlammadinger
S
S
Sipka
A
A
Kiss
G
G
Szegedi
K
K
Rák
G
G
Szabó
D001692:Biological Transport; D002454:Cell Differentiation; D002455:Cell Division; D003561:Cytarabine / Q000494:pharmacology; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000494:pharmacology*; D018014:Gene Transfer Techniques; D006098:Granulocytes / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D007963:Leukocytes, Mononuclear; D009838:Oligodeoxyribonucleotides / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D004268:DNA-Binding Proteins; C050988:DUSF protein, Neurospora crassa; D005656:Fungal Proteins; D009838:Oligodeoxyribonucleotides; D003561:Cytarabine; D013755:Tetradecanoylphorbol Acetate; D006918:Hydroxyurea