Rapid preparation of plasma membranes from avian lymphoid cells and fibroblasts for virus binding studies.
A simple and rapid protocol for the preparation of plasma membranes from chicken embryo fibroblasts and chicken lymphoid cells was developed. Characterization of the preparations by morphological, biochemical and serological methods indicated the specific enrichment of the plasma membranes as well as cell surface proteins. Binding of infectious bursal disease virus (IBDV) particles was demonstrated after immobilization of the plasma membranes, and cell type-specific differences were observed. Although the results of these studies reflect the interaction between IBDV and isolated cells only partially, the advantages of these plasma membrane preparations, the specific enrichment of cell surface proteins, their constant quality and the possibility to store aliquots over several months, make them a useful tool for virus binding studies with avian cells.
Universität Leipzig, Veterinärmedizinische Fakultät, Institut für Virologie, Germany. virology@vetmed.uni-leipzig.de
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Nieper
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Müller
D000818:Animals; D002462:Cell Membrane / Q000821:virology*; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000821:virology*; D005347:Fibroblasts / Q000648:ultrastructure / Q000821:virology; D006801:Humans; D007243:Infectious bursal disease virus / Q000276:immunology / Q000502:physiology*; D008214:Lymphocytes / Q000648:ultrastructure / Q000821:virology; D013047:Specific Pathogen-Free Organisms