Identification of sucrose synthase as an actin-binding protein.
Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.
Winter
H
H
US Department of Agriculture, Agricultural Research Service, Raleigh, NC, USA.
Huber
J L
JL
Huber
S C
SC
D000199:Actins / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003902:Detergents; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008840:Microfilament Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D017830:Octoxynol; D010766:Phosphorylation; D011817:Rabbits; D012710:Serum Albumin, Bovine; D012995:Solubility; D003313:Zea mays / Q000201:enzymology
D000199:Actins; D003902:Detergents; D008840:Microfilament Proteins; D012710:Serum Albumin, Bovine; D017830:Octoxynol; D005964:Glucosyltransferases; C022699:sucrose synthase; D005634:Fructose-Bisphosphate Aldolase