Transcription of the groESL operon in Pseudomonas aeruginosa PAO1.
Northern hybridization and S1 nuclease mapping demonstrated that the groES and groEL genes in Pseudomonas aeruginosa were transcribed as a bicistronic mRNA of 2.2 kb. Two transcription start sites and a transcription termination site were mapped. Overlapping consensus sequences for sigma 32- and sigma 70-dependent promoters were found in the upstream region of groES. Levels of groESL-specific mRNA were increased about 2-fold upon heat shock. This response differs from the dramatic enhancement (more than 10-fold) of groESL transcription after heat shock observed in other bacterial species.
Radioisotope Center, National Institute of Genetics, Mishima, Shizuoka, Japan. mafujita@lab.nig.ac.jp
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Fujita
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Amemura
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Aramaki
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D018835:Chaperonin 10 / Q000235:genetics; D018833:Chaperonins / Q000235:genetics*; D002874:Chromosome Mapping; D018869:Heat-Shock Response; D008969:Molecular Sequence Data; D009876:Operon / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D018835:Chaperonin 10; C097970:GroESL protein, Bacteria; D012333:RNA, Messenger; D012260:Ribonucleases; D018833:Chaperonins