Spectral sensitivity of melatonin synthesis suppression in Xenopus eyecups.
Melatonin synthesis in retinal photoreceptors is stimulated at night by a circadian oscillator and suppressed acutely by light. To identify photoreceptor mechanisms involved in the acute suppression of melatonin synthesis, an action spectrum was measured for dark-adapted Xenopus laevis eyecups at night. Intensity-response curves at six wavelengths from 400 to 650 nm were parallel, suggesting that a single photopigment predominates in melatonin suppression. Half-saturating intensities at 400, 440, 480, and 533 nm were not significantly different from one another, at 1-2 x 10(8) quanta cm(-2) s(-1). Significantly higher intensities of 580- and 650-nm light were required for melatonin suppression. These results indicate a predominant role for the principal green-absorbing rods in acute regulation of retinal melatonin synthesis in response to light, and argue against an important role for the red-absorbing cones. Higher than expected sensitivity at short wavelengths suggests that photoreceptors sensitive to blue and/or violet light may also contribute to melatonin suppression.
Cahill
G M
GM
Department of Biology, University of Houston, TX 77204-5513, USA.
Parsons
S E
SE
Besharse
J C
JC
D000818:Animals; D002940:Circadian Rhythm; D003623:Dark Adaptation; D008027:Light; D008297:Male; D008550:Melatonin / Q000096:biosynthesis*; D010786:Photoreceptor Cells / Q000502:physiology*; D012684:Sensory Thresholds; D014981:Xenopus; D014982:Xenopus laevis
D008550:Melatonin