Infectious pancreatic necrosis virus (IPNV) does not require acid compartments for entry into cells.
The early events in the infection of unenveloped viruses are still rather unknown and puzzling. However, as in the case of enveloped viruses, the acid pH of endosomes can be important to trigger the virus entry into the cytosol. To test if the infectious pancreatic necrosis virus (IPNV), an unenveloped virus, requires acid endosomal pH to infect cells, we assayed the effect of Bafilomycin A1 on IPNV replication. Concentrations of the antibiotic which inhibited the endosomal acidification of the host cells were unable to affect IPNV replication in CHSE-214 cells; therefore, the acid pH of endosomes seems not to be a mandatory condition for the entry of IPNV into cells.
Laboratorio de Bioquímica y Virología, Facultad de Ciencias, Universidad de Valparaíso, Chile.
J C
JC
Espinoza
J
J
Kuznar
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D006863:Hydrogen-Ion Concentration; D018061:Infectious pancreatic necrosis virus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018942:Macrolides; D006180:Proton-Translocating ATPases / Q000037:antagonists & inhibitors; D012474:Salmon
D000900:Anti-Bacterial Agents; D018942:Macrolides; C040929:bafilomycin A1; D006180:Proton-Translocating ATPases