Cloning and sequencing of the bovine STAT5A cDNA reveals significant sequence divergence with ovine.
The transcription factors STAT5 mediates prolactin signals in mammary epithelial cells. The cDNA of bovine STAT5A was cloned, sequenced and compared to other species. The encoded protein proves to be > 95% homologous to other mammals. We show that the STAT5A mRNA of the closely related ovine species contains an extended (by 130 nt) 5'-untranslated region, being encoded by an extra-exon, and accounts, possibly, for improved translation efficiency.
Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, Germany.
P
P
Schröder
L
L
Meyer
T T
TT
Wheeler
H J
HJ
Thiesen
H M
HM
Seyfert
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000502:physiology; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D005260:Female; D008894:Milk Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D050799:STAT5 Transcription Factor; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*; D013045:Species Specificity; D015534:Trans-Activators / Q000235:genetics* / Q000502:physiology
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008894:Milk Proteins; D012333:RNA, Messenger; D050799:STAT5 Transcription Factor; D015534:Trans-Activators