High concentration of glucose induces the expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells.
Atherosclerosis is known to be accelerated in patients with diabetes mellitus. We have examined the effect of glucose on the expression of intercellular adhesion molecule-1 (ICAM-1) in cultured human umbilical vein endothelial cells (HUVEC) and the adhesion of cells of monocyte-like cell line, THP-1, to HUVEC. HUVEC exposed to a high glucose concentration (16.7 mM) showed a 1.4-fold increase in the adhesion of THP-1 cells and a 1.3-fold increase in cell surface expression of ICAM-1 after 6 h exposure compared with those cultured in medium with a low glucose concentration (5.6 mM). ICAM-1 expression began to increase after 3 h exposure, was maximal at 6 h and gradually decreased afterwards. At 16.7 mM, raffinose stimulation produced a significantly lower expression of ICAM-1 on HUVEC than glucose, furthermore it caused a significantly lower expression than low glucose stimulation (5.6 mM). We conclude that a high concentration of glucose can induce ICAM-1 in endothelial cells and that this effect may play an important role in atherogenesis in patients with diabetes mellitus.
Second Department of Internal Medicine, Osaka University Medical School, Suita, Japan. shizu@imed2.med.osaka-u.ac.jp
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Takami
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Yamashita
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Kihara
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Kameda-Takemura
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Matsuzawa
D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005947:Glucose / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects / Q000502:physiology; D009994:Osmolar Concentration; D011887:Raffinose / Q000494:pharmacology; D013997:Time Factors; D014471:Umbilical Veins / Q000378:metabolism*
D003470:Culture Media; D018799:Intercellular Adhesion Molecule-1; D005947:Glucose; D011887:Raffinose