Use of the lactococcal nisA promoter to regulate gene expression in gram-positive bacteria: comparison of induction level and promoter strength.
We characterized the regulated activity of the lactococcal nisA promoter in strains of the gram-positive species Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Enterococcus faecalis, and Bacillus subtilis. nisA promoter activity was dependent on the proteins NisR and NisK, which constitute a two-component signal transduction system that responds to the extracellular inducer nisin. The nisin sensitivity and inducer concentration required for maximal induction varied among the strains. Significant induction of the nisA promoter (10- to 60-fold induction) was obtained in all of the species studied at a nisin concentration just below the concentration at which growth is inhibited. The efficiency of the nisA promoter was compared to the efficiencies of the Spac, xylA, and lacA promoters in B. subtilis and in S. pyogenes. Because nisA promoter-driven expression is regulated in many gram-positive bacteria, we expect it to be useful for genetic studies, especially studies with pathogenic streptococci in which no other regulated promoters have been described.
Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, Georgia 30322, USA.
Z
Z
Eichenbaum
M J
MJ
Federle
D
D
Marra
W M
WM
de Vos
O P
OP
Kuipers
M
M
Kleerebezem
J R
JR
Scott
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001426:Bacterial Proteins / Q000502:physiology; D001483:Base Sequence; D004305:Dose-Response Relationship, Drug; D015964:Gene Expression Regulation, Bacterial; D005966:Glucuronidase / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D015398:Signal Transduction; D013289:Streptococcaceae / Q000187:drug effects / Q000235:genetics*; D014157:Transcription Factors
D001426:Bacterial Proteins; D014157:Transcription Factors; C080935:nisR protein, Lactococcus lactis; D009561:Nisin; D005966:Glucuronidase