Induction of T cell dependent acquired immunity in syngeneic mice by the combined expression of interleukin-4 and granulocyte macrophage-colony stimulating factor gene in murine colon carcinoma cells.
The inoculation of cytokine-producing tumor cells into syngeneic animals can produce antitumor effects. We previously reported that injection of a mixed population of murine colon carcinoma (Colon 26) cells transduced with interleukin-4 (IL-4) gene with granulocyte macrophage-colony stimulating factor (GM-CSF) gene developed subcutaneous tumors in syngeneic mice, but these tumors thereafter regressed spontaneously (Oncology 1997;54:69-73). In this study, we found the generation of tumor-specific acquired immunity in the mice which had rejected the tumors of a mixed population of IL-4 and GM-CSF producer cells, and that the immunity was mediated by mature T cells. Moreover, local secretion of both IL-4 and GM-CSF in the vicinity of tumors was found to be crucial for this rejection. These data suggest that enhanced host defense brought about by the combined expression of IL-4 and GM-CSF genes in tumor cells is a potential therapeutic strategy.
Division of Pathology, Chiba Cancer Center Research Institute, Japan.
K
K
Tasaki
Y
Y
Gunji
H
H
Matsubara
K
K
Takenaga
T
T
Suzuki
T
T
Asano
T
T
Ochiai
K
K
Isono
T
T
Kouzu
S
S
Sakiyama
M
M
Tagawa
D000818:Animals; D003110:Colonic Neoplasms / Q000276:immunology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D007111:Immunity, Cellular; D015847:Interleukin-4 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000096:biosynthesis; D015996:Survival Rate; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014162:Transfection; D014185:Transplantation, Isogeneic / Q000276:immunology
D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor