Glycoprotein composition in cyclophosphamide-induced lung fibrosis.
The present study investigated the glycosylation state of proteins in lung tissue of a cyclophosphamide-induced model of pulmonary fibrosis in rats. In fibrotic lung, the carbohydrate constituents (total hexose, fucose, sialic acid and hexosamine) of salt-soluble, collagenase, elastase and papain digested glycoproteins were significantly higher compared to normal lungs. Interestingly, fibrotic lung tissues had higher activities of mannosyl, glucosyl, galactosyl, sialyl and fucosyl transferases than normal lung tissues. Similarly, mannosyl, glucosyl, galactosyl, sialyl and fucosyl transferases were higher in serum from rats with fibrosis than in that from normals. These data indicate that glycoprotein metabolism is significantly altered from normal in animals with interstitial lung fibrosis.
Department of Biochemistry, Central Leather Research Institute, Madras 600 020, India. VNAR@MEAKINS.LAN.MCGILL.CA
N
N
Venkatesan
D
D
Punithavathi
G
G
Chandrakasan
D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D004195:Disease Models, Animal; D010450:Endopeptidases / Q000378:metabolism; D005355:Fibrosis / Q000139:chemically induced; D006023:Glycoproteins / Q000737:chemistry*; D016695:Glycosyltransferases / Q000032:analysis; D006909:Hydroxyproline / Q000378:metabolism; D008168:Lung / Q000201:enzymology / Q000473:pathology*; D008297:Male; D009005:Monosaccharides / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D006023:Glycoproteins; D009005:Monosaccharides; D003520:Cyclophosphamide; D016695:Glycosyltransferases; D010450:Endopeptidases; D006909:Hydroxyproline