Aberrant isoform of vascular endothelial growth factor 189 expression is correlated with xenotransplantability of human esophageal cancer.
Xenografted neoplastic tissues are often utilized to study biology of human cancer. We studied isoform pattern (VEGF206, VEGF189, VEGF165, VEGF121) of an angiogenic factor VEGF in both esophageal primary cancers and cancer xenografts by RT-PCR analysis. Eighteen of 19 (95%) xenografts showed the cell associated aberrant isoform of VEGF189 at a significantly higher incidence than 19 of 31 (61%) primary cancers (P=0.009, Fisher's exact test). These observations suggested that the aberrant isoform of VEGF189 mRNA affects the xenotransplantability of human esophageal cancer.
Tokunaga
T
T
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Kijima
H
H
Oshika
Y
Y
Fukushima
Y
Y
Abe
Y
Y
Ohnishi
Y
Y
Yamazaki
H
H
Tsuchida
T
T
Makuuchi
H
H
Tamaoki
N
N
Ueyama
Y
Y
Nakamura
M
M
D000818:Animals; D016228:Endothelial Growth Factors / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D004938:Esophageal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D005091:Exons; D006801:Humans; D008222:Lymphokines / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D016513:Mice, SCID; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic; D014183:Transplantation, Heterologous; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors