MAG and MOG enhance neurite outgrowth of embryonic mouse spinal cord neurons.
Myelin-associated glycoprotein (MAG) inhibits neurite outgrowth of postnatal spinal cord neurons, but its effect on embryonic neurons is unknown. The effect on neurite outgrowth of another myelin protein, myelin-oligodendrocyte glycoprotein (MOG) is also unknown. We determined the effect of MAG and MOG on embryonic day 17 spinal cord neurons, which were cultured on MAG, MOG or control transfected CHO cells. Neurite outgrowth was examined and both total neurite length and longest neurite length were significantly enhanced by both MAG and MOG. These findings show that, in contrast to postnatal spinal cord neurons, MAG can enhance neurite outgrowth of embryonic spinal cord neurons. In addition, another myelin protein, MOG, can also modulate neurite outgrowth.
Development and Neurobiology Group, The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Victoria, Australia.
A M
AM
Turnley
P F
PF
Bartlett
D000818:Animals; D016466:CHO Cells; D002531:Cerebellum / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D006224:Cricetinae; D019922:GAP-43 Protein / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D051379:Mice; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000494:pharmacology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D016501:Neurites / Q000187:drug effects / Q000648:ultrastructure*; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure*; D009836:Oligodendroglia / Q000187:drug effects / Q000648:ultrastructure*; D013116:Spinal Cord / Q000187:drug effects / Q000254:growth & development / Q000648:ultrastructure*; D013268:Stimulation, Chemical; D014162:Transfection
D019922:GAP-43 Protein; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein