137Cesium-induced chromosome aberrations analyzed by fluorescence in situ hybridization: eight years follow up of the Goiânia radiation accident victims.
The radiation accident in focus here occurred in a section of Goiânia (Brazil) where more than a hundred individuals were contaminated with 137Cesium on September 1987. In order to estimate the absorbed radiation doses, initial frequencies of dicentrics and rings were determined in 129 victims [A.T. Ramalho, PhD Thesis, Subsidios a tecnica de dosimetria citogenetica gerados a partir da analise de resultados obtidos com o acidente radiologico de Goiânia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil, 1992]. We have followed some of these victims cytogenetically over the years seeking for parameters that could be used as basis for retrospective radiation dosimetry. Our data on translocation frequencies obtained by fluorescence in situ hybridization (FISH) could be directly compared to the baseline frequencies of dicentrics available for those same victims. Our results provided valuable information on how precise these estimates are. The frequencies of translocations observed years after the radiation exposure were two to three times lower than the initial dicentrics frequencies, the differences being larger at higher doses (>1 Gy). The accuracy of such dose estimates might be increased by scoring sufficient amount of cells. However, factors such as the persistence of translocation carrying lymphocytes, translocation levels not proportional to chromosome size, and inter-individual variation reduce the precision of these estimates.
LUMC, Leiden University Medical Centrum, Department of Radiation Genetics and Chemical Mutagenesis, Wassenaarseweg 72, 2333 AL Leiden, Netherlands. natarajan@rullf2medfac.leidenuniv.nl
A T
AT
Natarajan
S J
SJ
Santos
F
F
Darroudi
V
V
Hadjidikova
S
S
Vermeulen
S
S
Chatterjee
M
M
Berg
M
M
Grigorova
E T
ET
Sakamoto-Hojo
F
F
Granath
A T
AT
Ramalho
M P
MP
Curado
D001938:Brazil; D002478:Cells, Cultured; D002588:Cesium Radioisotopes / Q000009:adverse effects*; D002869:Chromosome Aberrations / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D018788:Radioactive Hazard Release; D011874:Radiometry; D012189:Retrospective Studies; D014178:Translocation, Genetic / Q000528:radiation effects
D002588:Cesium Radioisotopes