[Indirect ultra-micro-ELISA for detecting IgG antibodies in patients with fascioliasis].
The standardization of an ultramicroELISA for the detection of IgG antibodies anti excretory-secretory antigens of Fasciola hepatica (UME-Fasciola) is described. It was studied a considerable group of sera of which 56 were from patients with fascioliasis, 168 from patients with other parasitic diseases, and 300 from sound persons that were used as negative controls. As regards the parasitology test considered as "Gold Standard", the UME-Fasciola showed a sensitivity of 100%, an specificity of 98%, and predictive values for positives and negatives of 90.3% and 100%, respectively. Cross-reaction was only observed with the sera from patients infected with Opistorchis felineus. On comparing the UME-Fasciola with the conventional ELISA, it was obtained a concordance index of 95.5%.
Instituto de Medicina Tropical Pedro Kourí, Ciudad de La Habana, Cuba.
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Espino
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Duménigo
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D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008832:Microchemistry; D010272:Parasitic Diseases / Q000097:blood / Q000276:immunology; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D007074:Immunoglobulin G