Immune response to baculovirus expressed protein fragment amino acids 190-289 of respiratory syncytial virus (RSV) fusion protein.
At least two neutralizing epitopes have been identified in the amino acid (aa) sequence 190-289 of the RSV fusion protein. The authors expressed this region in insect cells (bF190-289) and compared the immune response to bF190-289 with that induced by baculovirus expressed full-length fusion protein (bF). As with bF, mice primed with bF190-289 produced exclusively antibodies of IgG1 isotype, generated neutralizing antibodies, reduced significantly the virus titer (about a half log10 reduction) after RSV challenge and induced a Helper T (Th) 2 cell response in mediastinal lymph node cells (MLNC) restimulated in vitro. Thus, the aa sequence 190-289 represents a major immunogenic region of the RSV fusion protein.
Laboratoire de Microbiologie Médicale et Moléculaire, Facultés de Médecine et de Pharmacie, Dijon, France.
B
B
Werle
C
C
Bourgeois
A
A
Alexandre
V
V
Massonneau
P
P
Pothier
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D016367:Baculoviridae; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000276:immunology*; D012136:Respiratory Syncytial Viruses; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D010446:Peptide Fragments; D014760:Viral Fusion Proteins