Bioremediation of epsilon-caprolactam from nylon-6 waste water by use of Pseudomonas aeruginosa MCM B-407.
Nylon-6, a man-made polymer that finds its application in the manufacture of car tires, ropes, fabrics, automobile parts etc., is manufactured with epsilon-caprolactam. Waste water generated during production of nylon-6 contains the unreacted monomer. Owing to the polluting and toxic nature of epsilon-caprolactam, its removal from waste streams is necessary. Pseudomonas aeruginosa MCM B-407 was isolated from activated sludge used to treat waste from a factory producing nylon-6. This organism was able to remove epsilon-caprolactam with simultaneous reduction in chemical oxygen demand (COD). The degradation of epsilon-caprolactam in waste water was found to be optimal over a wide range of pH from 5.0 to 9.0, temperature of 30 degrees C, and under shake or aerated conditions, with an inoculum density of 10(5) cells/ml and with an incubation period of 24 - 48 h. Thus, Pseudomonas aeruginosa MCM B-407 isolated from the activated sludge exposed to epsilon-caprolactam may play an important role in the bioremediation of epsilon-caprolactam from the waste waters of industries manufacturing nylon-6.
Kulkarni
R S
RS
Division of Microbial Sciences, Agharkar Research Institute, G.G. Agarkar Road, Pune 411 004, India.
Kanekar
P P
PP
D002209:Caprolactam / Q000031:analogs & derivatives* / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014876:Water Pollution
D007220:Industrial Waste; D002209:Caprolactam