Binding of naturally occurring anti-DNA antibodies to estradiol.
Binding of naturally occurring human anti-DNA autoantibodies to beta-estradiol and native DNA has been investigated. Sera from seven SLE patients were tested for their reactivity with beta-estradiol and native calf thymus DNA. Solid Phase enzyme immunoassay was performed using both colorigenic and fluorogenic substrates. The results show enhanced binding of human anti-DNA autoantibodies to beta-estradiol as compared to native DNA. This was further substantiated by increased binding of affinity purified SLE IgG to beta-estradiol. These results suggest a role for female sex hormone in the etiopathogenesis of SLE and are significant vis-a-vis female predominance of SLE.
Moinuddin
Department of Biochemistry, Faculty of Medicine, A.M.U., Aligarh, India.
Ali
A
A
D000974:Antibodies, Antinuclear / Q000276:immunology* / Q000302:isolation & purification; D000915:Antibody Affinity; D004247:DNA / Q000276:immunology; D004958:Estradiol / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D012016:Reference Values
D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D004958:Estradiol; D004247:DNA