The effect of gramicidin, a topical contraceptive and antimicrobial agent with anti-HIV activity, against herpes simplex viruses type 1 and 2 in vitro.
The effect of an anti-HIV compound, gramicidin, previously used as a topical antibiotic and vaginal contraceptive, on the replication of herpes simplex viruses (HSV) type 1 and 2 has been examined. Human WI-38 fibroblasts were inoculated with either HSV type in the presence of serial dilutions of gramicidin and reduction in viral yield was measured by ELISA. The 50% inhibitory dose (IC50) of gramicidin against 3 HSV-1 and 4 HSV-2 isolates was equal to 0.3 microgram/ml and was comparable to the efficacy of the anti-HSV agent acyclovir (ACV). The IC50 of gramicidin required to protect WI-38 from cytolytic effect of HSV was 10 micrograms/ml at day 5 postinfection, indicating that at this time point the activity of gramicidin was inferior than that of ACV. Nevertheless, gramicidin suppressed the replication of ACV-resistant thymidine kinase and DNA polymerase HSV mutants at doses effective against ACV-sensitive strains. The results suggest that the antimicrobial and spermostatic agent, gramicidin, has potential against sexually transmitted diseases (STDs) and for prophylaxis of sex-borne HIV and HSV infections.
Bourinbaiar
A S
AS
Metatron, Inc., New York, New York, USA.
Coleman
C F
CF
D019380:Anti-HIV Agents / Q000494:pharmacology; D000891:Anti-Infective Agents, Local / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003271:Contraceptive Agents, Female / Q000494:pharmacology; D006096:Gramicidin / Q000494:pharmacology*; D018259:Herpesvirus 1, Human / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D018258:Herpesvirus 2, Human / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D006801:Humans
D019380:Anti-HIV Agents; D000891:Anti-Infective Agents, Local; D000998:Antiviral Agents; D003271:Contraceptive Agents, Female; D006096:Gramicidin