Mapping of 55 new rat microsatellite markers from chromosome-specific libraries.
Fifty-five novel rat microsatellite markers were isolated from libraries specific for rat chromosomes (Chrs) 1, 2, and 7. The markers were mapped in three backcross rat populations. Thirty of these markers mapped to Chrs 1, 2, or 7, while the other 25 mapped to other chromosomes. New markers for two genes, liver-specific transporter gene (Livtr) and insulin-responsive glucose transporter (Glut4), were also mapped to rat Chrs 9 and 10, respectively. Three provisionally assigned markers from previous studies were also confirmed. Detailed methodologies for the generation and enrichment of clones containing repeat sequences and for the isolation of chromosome-specific markers are presented, since they represent unique combinations and modifications of previous protocols. Such methods and the newly presented markers should be useful for both specific and general mapping studies in the rat.
Department of Human Oncology, University of Wisconsin-Madison, K4/334 CSC, 600 Highland Avenue, Madison, Wisconsin, USA.
L A
LA
Shepel
H
H
Lan
G M
GM
Brasic
M E
ME
Gheen
L C
LC
Hsu
J D
JD
Haag
M N
MN
Gould
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D015723:Gene Library; D008040:Genetic Linkage; D005819:Genetic Markers; D051275:Glucose Transporter Type 4; D008099:Liver / Q000378:metabolism; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics; D009124:Muscle Proteins; D016133:Polymerase Chain Reaction; D051381:Rats; D011916:Rats, Inbred F344 / Q000235:genetics; D011919:Rats, Inbred Strains / Q000235:genetics*; D011920:Rats, Inbred WF / Q000235:genetics; D011921:Rats, Inbred WKY / Q000235:genetics
D002352:Carrier Proteins; D005819:Genetic Markers; D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; C495694:Slc2a4 protein, rat