Development of an ELISA for pantothenic acid (vitamin B5) for application in the nutrition and biological fields.
Immunological assays appear to be the only alternative to the microbiological method for analysis of pantothenic acid in foods and blood. In order to evaluate the influence of the linker on the immunogenicity of the hapten, we have tried to raise antisera against pantothenic acid in rabbits using different conjugates. The hapten was coupled to a carrier protein (BSA or thyroglobulin) using adipoyl dichloride (adipoyl conjugate) or bromoacetyl bromide (acetyl conjugate). Only the acetyl conjugate has induced the production of a specific antibody. With this antibody, an assay on microplate using the ELISA inhibition technique was developed to measure pantothenic acid. The use of pantothenic acid coupled to thyroglobulin with adipoyl dichloride as the capture antigen has improved the sensitivity of the ELISA. This assay was applied to food products and blood.
Département de Santé Publique; Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
A
A
Gonthier
P
P
Boullanger
V
V
Fayol
D J
DJ
Hartmann
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002845:Chromatography; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis; D006241:Haptens; D007106:Immune Sera; D016014:Linear Models; D010205:Pantothenic Acid / Q000032:analysis* / Q000097:blood / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine; D013954:Thyroglobulin
D000906:Antibodies; D006241:Haptens; D007106:Immune Sera; D010205:Pantothenic Acid; D012710:Serum Albumin, Bovine; D013954:Thyroglobulin