Effects of transforming growth factor alpha and hepatocyte growth factor on acid-induced damage in rat gastric epithelial cells.
We examined the effects of transforming growth factor alpha (TGF-alpha) and hepatocyte growth factor (HGF) on acid-induced damage in a rat gastric epithelial cell line. Pretreatment of cells for 30 min with TGF-alpha, but not HGF, significantly prevented the acid-induced cell damage in a concentration-dependent manner. Genistein significantly reduced the protective effect of TGF-alpha. DNA synthesis in RGM1 cells was increased when the cells were incubated with TGF-alpha and HGF for 24 hr. We conclude that the protective effect of TGF-alpha against acid-induced damage seems to be caused by the activation of Na+/H+ exchangers and not by enhanced DNA synthesis.
Department of Applied Pharmacology, Kyoto Pharmaceutical University, Yamashina, Japan.
O
O
Furukawa
H
H
Matsui
N
N
Suzuki
S
S
Okabe
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects*; D019833:Genistein / Q000008:administration & dosage / Q000494:pharmacology; D006131:Growth Inhibitors / Q000008:administration & dosage / Q000494:pharmacology; D017228:Hepatocyte Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D016211:Transforming Growth Factor alpha / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014238:Trichloroacetic Acid / Q000009:adverse effects*
D006131:Growth Inhibitors; D016211:Transforming Growth Factor alpha; D014238:Trichloroacetic Acid; D017228:Hepatocyte Growth Factor; D004247:DNA; D019833:Genistein