The treA gene of Bacillus subtilis is a suitable reporter gene for the archaeon Methanococcus voltae.
The similarity of the transcriptional apparatus of Archaea with that of Eucarya makes studies of their transcriptional regulation especially interesting. Such investigations are greatly facilitated by reporter genes. The concomitant analysis of several promoters for investigations of regulatory patterns requires different reporter genes. The archaeon Methanococcus voltae is a moderately halophilic mesophile. The treA gene from Bacillus subtilis appeared to be a good candidate for a reporter, since its product trehalase is salt-resistant. We show that it is indeed expressed under the control of a M. voltae promoter and that the enzyme is easily testable in cell lysates.
Sniezko
I
I
Department of Biology, Philipps University, Marburg, Germany.
Dobson-Stone
C
C
Klein
A
A
D001412:Bacillus subtilis / Q000235:genetics*; D004186:Disaccharidases / Q000235:genetics* / Q000378:metabolism; D019848:Gene Expression Regulation, Archaeal; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors; D017017:Methanococcus / Q000201:enzymology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D004186:Disaccharidases; C040631:alpha, alpha-phosphotrehalase