Molecular analysis of childhood acute lymphoblastic leukemia in Israel.
Ninety-two Israeli children with acute lymphoblastic leukemia (ALL) (67 B-lineage and 25 T-lineage) were analyzed for the immunological antigen receptor gene configuration. Thirty-nine of the patients (27 B-lineage and 12 T-lineage) relapsed. The incidence of the identified rearrangements within the immunoglobulin heavy chain (IgH) and T-cell receptor (TCR)beta, gamma and delta genes, at diagnosis, was in accordance with previous studies from other countries. Furthermore, the clinical relevance of bi/oligoclonal status, at diagnosis, and clonal selection was determined in this long-term follow-up study (median 112 months). A similar relapse rate was observed among the B-lineage patients with bi/oligoclonal and monoclonal patterns indicated by IgH gene rearrangement. Based on our results, we suggest that bi/oligoclonality has no prognostic significance (P=0.8533). Clonal variations between diagnosis and subsequent relapses were detected in 60% (12/20) of the patients; 64% (7/11) B-lineage and 55% (5/9) T-lineage. Clonal selection significantly correlated with shorter duration of remission and earlier recurrence (P=0.0025).
Cancer Molecular Genetics, Felsenstein Medical Research Centre, Schneider Children's Medical Center of Israel, Petah Tiqva.
O
O
Blau
S
S
Avigad
A
A
Frisch
Y
Y
Kilim
B
B
Stark
Y
Y
Kodman
D
D
Luria
I J
IJ
Cohen
R
R
Zaizov
D000293:Adolescent; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D015139:Blotting, Southern; D019070:Cell Lineage / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002999:Clone Cells / Q000276:immunology / Q000502:physiology; D004247:DNA / Q000032:analysis; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain / Q000235:genetics; D015329:Gene Rearrangement, T-Lymphocyte / Q000235:genetics; D006801:Humans; D016130:Immunophenotyping; D015994:Incidence; D007223:Infant; D007557:Israel / Q000453:epidemiology; D008297:Male; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000453:epidemiology / Q000235:genetics*; D011379:Prognosis; D012008:Recurrence; D013601:T-Lymphocytes / Q000276:immunology / Q000502:physiology; D013997:Time Factors
D004247:DNA