The uvsI gene of Aspergillus nidulans required for UV-mutagenesis encodes a homolog to REV3, a subunit of the DNA polymerase zeta of yeast involved in translesion DNA synthesis.
Defects in the uvsI gene of Aspergillus nidulans resulted in high UV sensitivity and reductions of spontaneous and UV-induced reversion of certain alleles, uvsl;uvsA double mutants exhibited high methyl methane sulfonate (MMS)-sensitivity in contrast to the slight sensitivity of the component single mutants. Using such a double mutant as recipient, a clone complementing uvsI501 has been isolated from a chromosome III specific library. The deduced amino acid sequence from the 1.1-kb sequenced region, a part of the 5.2-kb DNA fragment showing uvsI-complementing activity, had a 62% identity with REV3 of yeast. Disruptants of the cloned gene demonstrated the same level of sensitivity to UV light as uvsI and failed to complement uvsI501 in heterozygous diploids.
Han
K Y
KY
Dept. of Molecular Biology, Wonkwang University, Iksan, South Korea.
Chae
S K
SK
Han
D M
DM
D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D019951:DNA Polymerase beta / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase; D005656:Fungal Proteins / Q000032:analysis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics* / Q000528:radiation effects; D008969:Molecular Sequence Data; D016296:Mutagenesis / Q000235:genetics / Q000528:radiation effects; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
D004271:DNA, Fungal; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D019951:DNA Polymerase beta; D004259:DNA-Directed DNA Polymerase; C092602:REV3 protein, S cerevisiae