[Solid-phase immunoenzyme ass for detecting Giardia lamblia antigens].
An ELISA sandwich for the detection of Giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from G. lamblia. The sensitivity of the technique was 94.8% and the specificity 98.3%. The method detects an antigen concentration of 31 ng. The procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.
Torres
D
D
Instituto de Medicina Tropical Pedro Kourí.
Fernández
M
M
Brito
T
T
Finlay
C
C
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005243:Feces / Q000469:parasitology; D016829:Giardia lamblia / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification; D005873:Giardiasis / Q000175:diagnosis / Q000469:parasitology; D006801:Humans; D011817:Rabbits; D012680:Sensitivity and Specificity
D000953:Antigens, Protozoan