Structural studies of the major polysaccharide in the cell wall of Renibacterium salmoninarum.
The galactose-rich polysaccharide (GPS) in the cell wall of the Gram-positive bacterium Renibacterium salmoninarum, the causative agent in of bacterial kidney disease (BKD) of salmonids, has been studied by sugar and methylation analysis, partial acid hydrolysis, Smith degradation, FABMS, and 1H and 13C NMR spectroscopy. The data show that the GPS has a heptasaccharide repeating unit with the following structure: alpha-D-Rhap-(1-->3)-alpha-L-FucpNAc-(1-->)-beta-D-GlcpNAc 1 decreases 2 -->3)-beta-D-Galf-(1-->6)-beta-D-Galf-(1-->3)-beta-D-Galf -(1-->6) -beta-D-Galf-(1-->.
Sørum
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U
Department of Marine Biochemistry, Norwegian College of Fishery Science, University of Tromsø, Norway.
Robertsen
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B
Kenne
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L
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002473:Cell Wall / Q000737:chemistry*; D005399:Fishes; D005690:Galactose / Q000032:analysis; D006094:Gram-Positive Bacteria / Q000737:chemistry* / Q000302:isolation & purification; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D005690:Galactose