Activation of c-jun and c-fos genes in dNTP imbalance cell death induced with 5-fluoro-2'-deoxyuridine in mouse mammary tumor FM3A cell line.
5-Fluoro-2' -deoxyuridine (FdUrd)-induced death of mouse mammary FM3A cells was found to be associated with an increased expression of cellular c-jun and c-fos genes. The increase in these gene expressions was mediated through the protein kinase C-dependent pathway. Blockage of the expressions with the use of antisense oligodeoxynucleotide for c-jun delayed the cell death. These findings suggest that the activation of c-jun and c-fos genes, which encode transcription factors participating in cell proliferation, plays a role in FdUrd-induced cell death.
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
T
T
Kakutani
Y
Y
Ebara
K
K
Kanja
K
K
Takahashi
Y
Y
Wataya
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D003853:Deoxyribonucleosides / Q000378:metabolism; D005260:Female; D005467:Floxuridine / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D016762:Genes, fos; D016758:Genes, jun; D008325:Mammary Neoplasms, Experimental / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D003853:Deoxyribonucleosides; D005467:Floxuridine