amlC, another amylolytic gene maps close to the amlB locus in Streptomyces lividans TK24.
The region located upstream of the alpha-amylase gene (amlB) of Streptomyces lividans TK24 (Yin et al., 1997) contains a 2978-bp-long ORF divergent from amlB, and designated amlC. amlC Encodes a 993amino acid (aa) protein with a calculated molecular weight of 107.054kDa. On the basis of sequence similarity as well as enzymatic activity, AmlC is likely to belong to the 1, 4-alpha-D-glucan glucanohydrolase family. amlC is transcribed as a unique 3kb leaderless monocistronic mRNA. Primer extension experiments allowed the identification of promoter sequences that do not resemble the typical eubacterial promoter sequences. amlC was successfully disrupted and was mapped at approx. 700kb from a chromosomal end of S. lividans TK24, 100kb on the right of the amplifiable unit AUD1 (Volff et al., 1996). Nevertheless, amlC disruption seemed to be accompanied by extensive rearrangements of the 2500-kb DraI-II fragment of the chromosome.
Laboratoire de Biologie et Génétique Moléculaire, Institut de Génétique et Microbiologie, CNRS URA D2225 Bâtiment 400, Université Paris-Sud, F-91405, Orsay, Cedex, France.
X H
XH
Yin
C
C
Gerbaud
F X
FX
Francou
M
M
Guérineau
M J
MJ
Virolle
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics; D015870:Gene Expression / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D005798:Genes, Bacterial / Q000235:genetics*; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000737:chemistry / Q000201:enzymology / Q000235:genetics*; D000516:alpha-Amylases / Q000235:genetics*
D001426:Bacterial Proteins; C112384:AmlC protein, Streptomyces lividans; D006026:Glycoside Hydrolases; D000516:alpha-Amylases