Stem cell factor influences mast cell mediator release in response to eosinophil-derived granule major basic protein.
Stem cell factor (SCF) is an important mast cell growth, differentiation, and survival factor. We investigated whether SCF influenced the response of mouse mast cells to an IgE-independent stimulus, eosinophil-derived granule major basic protein (MBP). Mouse bone marrow cultured mast cells (BMCMC) were derived in either concanavalin-stimulated mouse spleen conditioned medium (CM) or SCF. The cloned growth, factor-independent mast cell line Cl.MC/C57.1 was also studied. BMCMC in SCF exhibited cytochemical staining properties, protease and histamine content, and increased serotonin uptake consistent with more mature differentiated mast cells as compared with BMCMC in CM or Cl.MC/ C57.1 cells. BMCMC in SCF released serotonin, 14C-labeled arachidonic acid metabolites and tumor necrosis factor-alpha (TNF-alpha) on stimulation with MBP, while no response was seen from either BMCMC in CM or Cl.MC/C57.1 cells. All three mast cell populations released mediators on stimulation with the cationic MBP analog, poly-L-arginine, indicating that the cationic charge did not explain the selective response of BMCMC in SCF to eosinophil-derived granule MBP. These findings show that SCF significantly influences mast cell differentiation and the responsiveness of mast cells to eosinophil-derived granule MBP.
The Combined Program in Pediatric Gastroenterology and Nutrition, The Children's Hospital and Massachusetts General Hospital, Boston, MA, USA.
G T
GT
Furuta
S J
SJ
Ackerman
L
L
Lu
R E
RE
Williams
B K
BK
Wershil
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001798:Blood Proteins / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002454:Cell Differentiation; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D010450:Endopeptidases / Q000096:biosynthesis; D047091:Eosinophil Granule Proteins; D006632:Histamine / Q000032:analysis; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007476:Ionophores / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D051381:Rats; D012260:Ribonucleases; D012701:Serotonin / Q000378:metabolism*; D019089:Stem Cell Factor / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D001095:Arachidonic Acids; D001798:Blood Proteins; D017077:Culture Media, Conditioned; D047091:Eosinophil Granule Proteins; D007476:Ionophores; D010455:Peptides; D019089:Stem Cell Factor; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; C015462:polyarginine; D012701:Serotonin; D007073:Immunoglobulin E; D006632:Histamine; D012260:Ribonucleases; D010450:Endopeptidases; D002118:Calcium