Sensitive detection of trace aluminium in biological tissues by confocal laser scanning microscopy after staining with lumogallion.
This paper describes a method for the sensitive detection of aluminium in biological tissues by real time confocal laser scanning microscopy after staining with lumogallion. The method enabled detection of aluminium > or = 9 micrograms g-1 in bone and is more sensitive than the conventional histochemical methods with aluminium and solochrome azurine, etc. Lumogallion reacts specifically with aluminium to form a fluorescent complex so that the proposed method is useful for detection and identification of aluminium in tissues.
Uchiumi
A
A
National Institute of Material and Chemical Research, Ibaraki, Japan.
Takatsu
A
A
Teraki
Y
Y
D000535:Aluminum / Q000032:analysis*; D000818:Animals; D001557:Benzenesulfonates / Q000494:pharmacology*; D001842:Bone and Bones / Q000737:chemistry*; D004396:Coloring Agents; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008099:Liver / Q000737:chemistry*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar
D001557:Benzenesulfonates; D004396:Coloring Agents; C061331:4-chloro-3-(2,4-dihydroxyphenylazo)-2-hydroxybenzene-1-sulfonic acid; D000535:Aluminum