Physical assignment of two porcine microsatellites to the short arm of chromosome 1 by modified DISC-PCR.
Direct in situ single copy PCR (DISC-PCR) was modified to increase signal detection efficiency and physically assign two porcine microsatellites (MS), Sw552 and Sw137, to chromosome 1p at Flpter 0.1-0.2 and Flpter 0.01-0.2, respectively. These are the first two MS localized to chromosome 1p by an in situ method. The modifications of the DISC-PCR procedure included use of buffered formalin and pepsin as pre-reaction treatments, and tetramethylbenzidine (TMB) for signal detection. The detection efficiency of the modified method is twofold higher than that of the original technique.
Hu
J
J
Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA.
Troyer
D L
DL
D000818:Animals; D001710:Biotin / Q000737:chemistry; D002874:Chromosome Mapping / Q000662:veterinary; D004076:Digoxigenin / Q000737:chemistry; D006241:Haptens / Q000737:chemistry; D008677:Metaphase; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D013552:Swine / Q000235:genetics*
D006241:Haptens; D001710:Biotin; D004076:Digoxigenin