The thermosome from Methanopyrus kandleri possesses an NH4+-dependent ATPase activity.
The ATPase activity of the thermosome from a methanogen, Methanopyrus kandleri, was characterized in detail. In contrast to all other known chaperonins, enzymatic ATP hydrolysis was found to be strictly dependent on high levels of ammonium salts in vitro. The ths gene encoding the thermosome subunit from the hyperthermophilic M. kandleri was functionally expressed in Escherichia coli and the overproduced polypeptide was assembled into intact thermosome complexes in the mesophilic host. The recombinant particles could be purified by a simple two-step procedure including only one chromatographic step. Structural and biochemical properties of the recombinant protein were closely similar to those of the natural complex. Western blot analysis with an antiserum against the M. kandleri thermosome indicated the presence of at least two subfamilies of archaeal chaperonins.
Andrä
S
S
Lehrstuhl für Mikrobiologie, Universität Regensburg, Germany.
Frey
G
G
Jaenicke
R
R
Stetter
K O
KO
D000251:Adenosine Triphosphatases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D000907:Antibodies, Bacterial; D019843:Archaeal Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D018833:Chaperonins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D003001:Cloning, Molecular; D003429:Cross Reactions; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D019605:Euryarchaeota / Q000201:enzymology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013045:Species Specificity; D056405:Thermosomes; D014461:Ultracentrifugation
D000907:Antibodies, Bacterial; D019843:Archaeal Proteins; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D000251:Adenosine Triphosphatases; D018833:Chaperonins; D056405:Thermosomes