Colony size can be used to determine the MIC of fluconazole for pathogenic yeasts.
This report describes a new statistical method for estimating the MIC of fluconazole for yeasts pathogenic to humans. This method is based on comparison of the colony sizes on solid media containing different concentrations of fluconazole. By this method, the MICs of fluconazole for 10 yeast strains were comparable to results obtained by the standard method recommended by the National Committee for Clinical Laboratory Standards. This method is simple to perform and easy to interpret. The turnaround time is faster than other methods. The method should be applicable to the determination MICs of other antifungal drugs for yeasts.
Department of Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.
J
J
Xu
R
R
Vilgalys
T G
TG
Mitchell
D000935:Antifungal Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009181:Mycoses / Q000382:microbiology*; D015003:Yeasts / Q000187:drug effects* / Q000254:growth & development*
D000935:Antifungal Agents; D015725:Fluconazole