Is the direct quantitation of antibiotics in agar by high-performance liquid chromatography useful?
The direct quantification of antibiotics in agar allows one to study the quality of the agar matrix, the kinetics of diffusion and the bacteria-antibiotic interaction. Mueller-Hinton agar (MHA) plates from three manufacturers were tested using HPLC and the disc diffusion test of ceftazidime (CAZ). Notable differences in the chromatographic profiles of MHA plate extracts from OXOID, DID and Becton Dickinson (BD) were shown, with a higher CAZ concentration after 24 h a 6 mm in BD P. aeruginosa inoculated plates (5.1 +/- 1.7 micrograms/ml, n = 6) vs. OXOID and DID (1.6 +/- 0.3 micrograms/ml, n = 12). BD plates gave also a different inhibition zone diameter (26 +/- 0.5 mm, n = 3) with respect to DID and OXOID (29 +/- 0.5 mm, n = 3).
Laboratory of Chromatography and Separative Techniques, IRCCS H S. Raffaele, Milan, Italy.
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Vaiani
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D000362:Agar / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000494:pharmacology; D002442:Ceftazidime / Q000032:analysis / Q000494:pharmacology; D002511:Cephalosporins / Q000032:analysis / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000032:analysis*; D004058:Diffusion; D007700:Kinetics; D008826:Microbial Sensitivity Tests / Q000379:methods; D011550:Pseudomonas aeruginosa / Q000187:drug effects
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D003470:Culture Media; D000362:Agar; D002442:Ceftazidime