Siderophore production by Aeromonas salmonicida subsp. salmonicida. Lack of strain specificity.
Siderophore production, presence of iron-regulated outer membrane proteins and siderophore specificity was determined among 17 isolates of Aeromonas salmonicida subsp. salmonicida obtained from Spain and Scotland. All grew in the presence of ethylenediamine di(o-hydroxyphenylacetic acid) (EDDA) and siderophore production was detected using chrome azurol S (CAS) agar, confirming the presence of a high-affinity siderophore iron-uptake mechanism. The Arnow test confirmed that all isolates produced a catechol siderophore. Cross-feeding assays with indicator bacteria showed the absence of anguibactin, enterobactin, 2,3-dihydroxybenzoic acid (DHBA) and the hydroxamate siderophore, aerobactin, in the iron-restricted supernants of a representative isolate which cross fed 15/17 A. salmonicida isolates tested. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed the presence of the same 2 major iron-regulated outer membrane proteins (IROMPs) in all isolates when grown in iron-restricted conditions and siderophore strain specificity as assessed by cross-feeding experiments was not apparent. Thus, with respect to IROMP and siderophore production A. salmonicida appears to be a homogeneous species.
Dpto Biología Funcional y Ciencias de la Salud, Facultad de Ciencias, Universidad de Vigo, Spain.
A I
AI
Fernandez
A F
AF
Fernández
M J
MJ
Pérez
T P
TP
Nieto
A E
AE
Ellis
D000333:Aeromonas / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis; D002614:Chelating Agents / Q000378:metabolism; D003470:Culture Media; D004492:Edetic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D005393:Fish Diseases / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D007501:Iron / Q000378:metabolism; D012487:Salmonidae / Q000382:microbiology*; D017262:Siderophores / Q000096:biosynthesis*; D013045:Species Specificity
D001425:Bacterial Outer Membrane Proteins; D002614:Chelating Agents; D003470:Culture Media; D017262:Siderophores; C026058:EDDA; D004492:Edetic Acid; D007501:Iron