Trisomy 12 in chronic lymphocytic leukemia--geographical variation.
Incidence of trisomy 12 was studied in 60 cases of chronic lymphocytic leukemia (CLL) with chromosome 12 specific alpha-satellite DNA probe by fluorescence in situ hybridization (FISH). Trisomy 12 was observed in 37 (61.8%) patients. Cells with trisomy 12 were detected in a varying proportion, ranging from > 2% to 86%. Patients with trisomy 12 were predominantly observed with total white blood cell (WBC) count > 80 x 10(9) l(-1) (P < 0.001). In addition, the percentage of trisomy 12 positive lymphocytes correlated with the high WBC counts. Trisomy 12 was observed equally in typical and atypical CLL. 90% of our patients were in the intermediate and high risk groups. It was seen that there was significantly higher percentage of trisomy 12 positive lymphocytes ( > 10%) in the high risk groups (P < 0.05). A higher incidence of FMC7 positivity in atypical CLL was seen in our study. However, there was no significant relationship found between trisomy 12 positivity and expression of either FMC7 or CD23 in our cases. It appears that the CLL that we see at our centre is at a different phase of evolution and perhaps biologically different compared to the CLL seen in the West.
Tata Memorial Hospital, Parel Mumbai, India. drnair@poboxes.com
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CN
Nair
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A
Chougule
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S
Dhond
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R
Goyal
P M
PM
Parikh
S
S
Pai
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Telang
S H
SH
Advani
D000328:Adult; D000367:Age Factors; D000368:Aged; D018956:CD5 Antigens / Q000032:analysis; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015994:Incidence; D007194:India / Q000453:epidemiology; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000145:classification / Q000453:epidemiology / Q000235:genetics*; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008875:Middle Aged; D017455:Receptors, IgE / Q000032:analysis; D014314:Trisomy
D018956:CD5 Antigens; D006023:Glycoproteins; C112523:MS4A1 protein, human; D017455:Receptors, IgE