Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm.
Zebrafish paraxial protocadherin (papc) encodes a transmembrane cell adhesion molecule (PAPC) expressed in trunk mesoderm undergoing morphogenesis. Microinjection studies with a dominant-negative secreted construct suggest that papc is required for proper dorsal convergence movements during gastrulation. Genetic studies show that papc is a close downstream target of spadetail, gene encoding a transcription factor required for mesodermal morphogenetic movements. Further, we show that the floating head homeobox gene is required in axial mesoderm to repress the expression of both spadetail and papc, promoting notochord and blocking differentiation of paraxial mesoderm. The PAPC structural cell-surface protein may provide a link between regulatory transcription factors and the actual cell biological behaviors that execute morphogenesis during gastrulation.
Howard Hughes Medical Institute, Department of Biological Chemistry, University of California, Los Angeles, California 90095-1662, USA.
A
A
Yamamoto
S L
SL
Amacher
S H
SH
Kim
D
D
Geissert
C B
CB
Kimmel
E M
EM
De Robertis
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics; D015820:Cadherins / Q000235:genetics* / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005775:Gastrula / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox; D017403:In Situ Hybridization; D008648:Mesoderm / Q000166:cytology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009024:Morphogenesis / Q000235:genetics; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D019170:Somites / Q000166:cytology; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics* / Q000502:physiology
D015820:Cadherins; D017931:DNA Primers; D014157:Transcription Factors