Characterization of a 200 kDa glycoprotein (Cs-gp200) on the pathogenic piscine haemoflagellate Cryptobia salmositica.
The 200 kDa antigenic doublet of the pathogenic haemoflagellate Cryptobia salmositica Katz, 1951, was detected using a monoclonal antibody (MAb-001) in 1-dimensional SDS-PAGE. This antigenic doublet is a glycoprotein since it was susceptible to both protease K and to sodium m-periodate oxidation and is designated Cs-gp200. It has a PI (isoelectric point) value of about 5.5 (using 2-dimensional gel electrophoresis). It migrated faster under reducing conditions than under nonreducing conditions and was partitioned into the aqueous phase in Triton X-114 phase separation. It is a secretory-excretory product since it was detected in a non-protein culture medium with C. salmositica. These results suggest that the Cs-gp200 is a glycoprotein consisting of carbohydrate determinants and conformational polypeptide with internal disulphide bonds. It is a hydrophilic antigen, is a secretory product of the parasite, and plays a important role in antibody elicitation in immunized fish.
Department of Zoology, University of Guelph, Ontario, Canada.
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology; D015153:Blotting, Western; D003902:Detergents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000378:metabolism; D005393:Fish Diseases / Q000469:parasitology*; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D006825:Hybridomas; D007106:Immune Sera / Q000276:immunology; D007526:Isoelectric Point; D051379:Mice; D008970:Molecular Weight; D017830:Octoxynol; D017686:Oncorhynchus mykiss / Q000469:parasitology*; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry; D011528:Protozoan Infections / Q000469:parasitology; D011529:Protozoan Infections, Animal; D015800:Protozoan Proteins / Q000737:chemistry* / Q000276:immunology; D012995:Solubility; D014351:Trypanosomatina / Q000737:chemistry / Q000276:immunology* / Q000472:pathogenicity
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D003902:Detergents; D006023:Glycoproteins; D007106:Immune Sera; D015800:Protozoan Proteins; D010504:Periodic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40; C009288:metaperiodate; D019286:Endopeptidase K