Detection and quantitation of cellularly derived amyloid beta peptides by immunoprecipitation-HPLC-MS.
A quantitative method for detection of amyloid beta peptides using immunoprecipitation-HPLC-mass spectrometry (IP-LC-MS) is described. Comparison of IP-LC-MS with sandwich ELISA revealed comparable results in the analysis of A beta 1-40 and A beta 1-42 derived from fetal guinea pig cell media and cell lysates. The use of IP-LC-MS not only allows a quantitative method for A beta 1-40 and A beta 1-42 peptides present in Alzheimer's disease (AD), but allows detection of other A beta peptide species that may also play a role in the onset of AD in humans.
Clarke
N J
NJ
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA.
Tomlinson
A J
AJ
Ohyagi
Y
Y
Younkin
S
S
Naylor
S
S
D016229:Amyloid beta-Peptides / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry; D002457:Cell Extracts; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000032:analysis*; D011233:Precipitin Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine
D016229:Amyloid beta-Peptides; D002457:Cell Extracts; D017077:Culture Media, Conditioned; D010455:Peptides