The course of enzymatically induced lipid peroxidation in homogenized porcine kidney tissue.
Homogenization of mammalian tissue--exemplified by porcine kidney-- causes enzymatically induced lipid peroxidation (LPO) processes proven by measuring the amounts of the typical lipid peroxidation products 9- and 13-hydroxy-octadecadienoic acid (HODE) either after homogenization in aqueous (activation of enzymes) or an organic (inactivated enzymes) solvent. A kinetic study revealed that the level of the 9- and 13-isomer reached maximum values 6 hours after tissue injury. Within one day the amount of these primary oxidation products was reduced fast, indicating that they undergo degradation in their biological environment. In contrast, the level of 10-hydroxy-octadecanoic acid--obviously derived from LPO of oleic acid--increased continuously even after one day. These observations reflect that the generation and degradation of hydroperoxides occurs at different rates which might be of interest in pathological processes connected with tissue injury, e.g. myocardial infarction.
Lehrstuhl Für Organische Chemie I, Universität Bayreuth, Germany.
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Kiessling
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Spiteller
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D007536:Isomerism; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008041:Linoleic Acids / Q000378:metabolism; D044243:Linoleic Acids, Conjugated; D015227:Lipid Peroxidation; D008322:Mammals; D019301:Oleic Acid / Q000378:metabolism; D013552:Swine
D008041:Linoleic Acids; D044243:Linoleic Acids, Conjugated; C024347:9-hydroxy-10,12-octadecadienoic acid; D019301:Oleic Acid; C024348:13-hydroxy-9,11-octadecadienoic acid