Microplate-array diagonal-gel electrophoresis (MADGE) and melt-MADGE: tools for molecular-genetic epidemiology.
Microplate-array diagonal-gel electrophoresis (MADGE) was invented for molecular-genetic epidemiological studies. It combines direct compatibility with microplates, convenient polyacrylamide-gel electrophoresis and economy of time and reagents at minimal capital cost, and enables one user to run up to several-thousand gel lanes per day for the direct assay of single-base variations. Melt-MADGE adds temporal-thermal-ramp apparatus to achieve similar throughput for de novo mutation scanning.
Wessex Human Genetics Institute, University of Southampton, UK. iday@hgmp.mrc.ac.uk
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Day
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Spanakis
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Palamand
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Weavind
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O'Dell
D004586:Electrophoresis / Q000379:methods*; D008967:Molecular Biology; D016133:Polymerase Chain Reaction