Isolation and characterization of S-layer proteins from a vent prosthecate bacterium.
MWapp 116,000 and 29,000 proteins (p116 and p29), major outer membrane proteins of Hyphomonas jannaschiana reproductive cells, were extracted from cell envelopes by dialysis against EDTA, 2 M urea or distilled water. These proteins were precipitated by divalent cations and resolubilized by EDTA-Na, reflecting alternate monomer, multimer states. From two-dimensional gel electrophoresis it was determined that p116 and p29 had a pl of 4.5. Both were glycoproteins. Results suggest that p116 and p29 are surface layer (S-layer) proteins, with p116 a tetramer of the p29. The S-layer could protect the adherent H. jannaschiana reproductive cell from exoenzyme activity, antibiotics and other bacteriocidal molecules produced in the bacterial films formed on many marine surfaces.
Shen
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Department of Microbiology, University of Maryland, College Park 20742, USA.
Weiner
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RM
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification*; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000648:ultrastructure; D007525:Isoelectric Focusing; D008854:Microscopy, Electron; D008970:Molecular Weight; D010140:Pacific Ocean
D001425:Bacterial Outer Membrane Proteins; D006023:Glycoproteins