Identification of the genes encoding the cytosolic translation release factors from Podospora anserina and analysis of their role during the life cycle.
In an attempt to decipher their role in the life history and senescence process of the filamentous fungus Podospora anserina, we have cloned the su1 and su2 genes, previously identified as implicated in cytosolic translation fidelity. We show that these genes are the equivalents of the SUP35 and SUP45 genes of Saccharomyces cerevisiae, which encode the cytosolic translation termination factors eRF3 and eRF1, respectively. Mutations in these genes that suppress nonsense mutations may lead to drastic mycelium morphology changes and sexual impairment but have little effect on life span. Deletion of su1, coding for the P. anserina eRF3, is lethal. Diminution of its expression leads to a nonsense suppressor phenotype whereas its overexpression leads to an antisuppressor phenotype. P. anserina eRF3 presents an N-terminal region structurally related to the yeast eRF3 one. Deletion of the N-terminal region of P. anserina eRF3 does not cause any vegetative alteration; especially life span is not changed. However, it promotes a reproductive impairment. Contrary to what happens in S. cerevisiae, deletion of the N terminus of the protein promotes a nonsense suppressor phenotype. Genetic analysis suggests that this domain of eRF3 acts in P. anserina as a cis-activator of the C-terminal portion and is required for proper reproduction.
Gagny
B
B
Institut de Génétique et Microbiologie, Université de Paris-Sud, 91405 Orsay cedex, France.
Silar
P
P
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D010454:Peptide Termination Factors / Q000235:genetics; D010641:Phenotype; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
D017931:DNA Primers; C489002:ETF1 protein, human; D005656:Fungal Proteins; D010454:Peptide Termination Factors; C087971:peptide-chain-release factor 3