Advances in fluorescence in situ hybridization.
The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide Signal Amplification and FISH with Polypeptide Nucleic Acid and Padlock probes.
Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, Netherlands. a.k.raap@mcb.medfac.leidenuniv.nl
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D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D002869:Chromosome Aberrations; D002874:Chromosome Mapping / Q000379:methods* / Q000639:trends*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000639:trends*; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides; D014439:Tyramine / Q000031:analogs & derivatives
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; C111551:biotinyltyramide; D001710:Biotin; D014439:Tyramine