Synthetic C-oligosaccharides mimic their natural, analogous immunodeterminants in binding to three monoclonal immunoglobulins.
The binding of three monoclonal antigalactan immunoglobulins, IgAs X24, J539 and X44 to their natural haptens methyl beta-D-galactopyranosyl-(1-->6)-beta-D-galactopyranoside, and the corresponding tri- and tetrasaccharides, was compared to the binding of these immunoglobulins with the comparable C-linked oligosaccharide analogues 1-3. The near identity of affinities of the two sets of oligosaccharides indicated the absence of any hydrogen bond involvement by the intersaccharidic oxygen atoms in the natural immunodeterminants.
Wang
J
J
Laboratory of Medicinal Chemistry, National Institutes of Health, Bethesda, MD 20892, USA.
Kovác
P
P
Sinaÿ
P
P
Glaudemans
C P
CP
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002240:Carbohydrate Sequence; D006241:Haptens / Q000276:immunology; D006860:Hydrogen Bonding; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism*; D018716:Molecular Mimicry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D006241:Haptens; D007070:Immunoglobulin A; D009844:Oligosaccharides