The effect of different doses of epidermal growth factor on liver ornithine decarboxylase and Na-K ATPase activities in newborn rats.
1. Ornithine decarboxylase and Na-K ATPase activities were studied in rat livers that were treated with different doses of epidermal growth factor (EGF). 2. The ornithine decarboxylase activities were studied with spectrophotometry, and results were expressed as micromoles of putrescine per hour per milligram of protein. Na-K ATPase activities were studied on the basis of the principle of measuring the amount of inorganic phosphates released by the hydrolysis of ATP, and the results were expressed as micromoles of inorganic phosphate per hour per milligram of protein. 3. When compared with the controls, although the Na-K ATPase activities were decreased at low doses of EGF, their activities were found to be increased at high doses of EGF. On the other hand, there was a positive correlation between ornithine decarboxylase activities and EGF doses. 4. The results of this study suggest that, whereas the decrease in Na-K ATPase activities at low doses of EGF can be due to the utilization of the enzyme, the increase in Na-K ATPase activities at high doses of EGF can be attributed to its enhanced synthesis.
Bilgihan
A
A
Department of Biochemistry, Gazi University School of Medicine, Ankara, Turkey.
Turkozkan
N
N
Isman
F
F
Kilinc
M
M
Demirsoy
S
S
D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D009955:Ornithine Decarboxylase / Q000187:drug effects*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects*
D004815:Epidermal Growth Factor; D009955:Ornithine Decarboxylase; D000254:Sodium-Potassium-Exchanging ATPase