Antisense oligonucleotide to insulin-like growth factor II induces apoptosis in human ovarian cancer AO cell line.
The effects of antisense oligonucleotide to insulin-like growth factor II (IGFII) to induce apoptosis in human ovarian cancer cells were evaluated. antiproliferation effects of antisense to IGFII in ovarian cancer AO cells were determined by 3H-thymidine incorporation. Apoptosis of the IGFII antisense-treated cells was quantitated by both nuclear condensation and flow cytometry after cells were stained with propidium iodide. IGFII antisense (4.5 microM) treatment of 48 h maximally inhibited proliferation of AO cells. More than 25% of IGFII antisense-treated cells (4.5 microM for 24 h) had undergone apoptosis, whereas less than 3% of the cells were apoptotic in either IGFII sense-treated cells or untreated cells. Antisense oligonucleotide to IGFII significantly inhibited cell proliferation and induced apoptosis in human ovarian cancer AO cell. These data suggest that IGFII may be a potential target in treatment of ovarian cancer and antisense oligonucleotide to IGFII may serve as a therapeutic approach.
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
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D017209:Apoptosis / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D016373:DNA, Antisense / Q000235:genetics / Q000494:pharmacology* / Q000627:therapeutic use; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000502:physiology; D008856:Microscopy, Fluorescence; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D014407:Tumor Cells, Cultured
D016373:DNA, Antisense; D004273:DNA, Neoplasm; D007335:Insulin-Like Growth Factor II