A methylated Neurospora 5S rRNA pseudogene contains a transposable element inactivated by repeat-induced point mutation.
In an analysis of 22 of the roughly 100 dispersed 5S rRNA genes in Neurospora crassa, a methylated 5S rRNA pseudogene, Psi63, was identified. We characterized the Psi63 region to better understand the control and function of DNA methylation. The 120-bp 5S rRNA-like region of Psi63 is interrupted by a 1.9-kb insertion that has characteristics of sequences that have been modified by repeat-induced point mutation (RIP). We found sequences related to this insertion in wild-type strains of N. crassa and other Neurospora species. Most showed evidence of RIP; but one, isolated from the N. crassa host of Psi63, showed no evidence of RIP. A deletion from near the center of this sequence apparently rendered it incapable of participating in RIP with the related full-length copies. The Psi63 insertion and the related sequences have features of transposons and are related to the Fot1 class of fungal transposable elements. Apparently Psi63 was generated by insertion of a previously unrecognized Neurospora transposable element into a 5S rRNA gene, followed by RIP. We name the resulting inactivated Neurospora transposon PuntRIP1 and the related sequence showing no evidence of RIP, but harboring a deletion that presumably rendered it defective for transposition, dPunt.
Margolin
B S
BS
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403, USA.
Garrett-Engele
P W
PW
Stevens
J N
JN
Fritz
D Y
DY
Garrett-Engele
C
C
Metzenberg
R L
RL
Selker
E U
EU
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics; D005800:Genes, Fungal; D008745:Methylation; D008969:Molecular Sequence Data; D009492:Neurospora crassa / Q000737:chemistry / Q000235:genetics*; D017354:Point Mutation; D011544:Pseudogenes; D012331:RNA, Fungal / Q000737:chemistry / Q000235:genetics*; D012341:RNA, Ribosomal, 5S / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004251:DNA Transposable Elements; D004271:DNA, Fungal; D012331:RNA, Fungal; D012341:RNA, Ribosomal, 5S