Analysis of hybridoma cell culture processes by SDS/gel capillary electrophoresis and matrix-assisted laser desorption ionization-time-of-flight MS.
SDS/gel capillary electrophoresis (SDS/gel CE) and matrix-assisted laser desorption ionization-time-of-flight MS (MALDI-TOF-MS) were employed to analyse changes in the culture broth during batch and continuous cultivation of hybridoma cells. The stability of IgG was analysed by SDS/gel CE and capillary zone electrophoresis (CZE). The results obtained by the new analytical procedures reflect the changes in the cultivation conditions very well, indicating that these tools can be used to follow animal cell culture processes. A new technique to collect fractions of very small volumes during the CZE separation is described, and the successful off-line coupling of CZE and MALDI-TOF-MS for the analysis of biotechnological processes is demonstrated.
Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, Jülich, Federal Republic of Germany.
V
V
Klyushnichenko
A
A
Rodenbrock
J
J
Thömmes
M R
MR
Kula
H
H
Heine
M
M
Biselli
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D002478:Cells, Cultured; D003470:Culture Media; D019075:Electrophoresis, Capillary / Q000379:methods*; D006825:Hybridomas / Q000166:cytology*; D007074:Immunoglobulin G / Q000032:analysis; D051379:Mice; D012710:Serum Albumin, Bovine / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D014168:Transferrin / Q000032:analysis
D003470:Culture Media; D007074:Immunoglobulin G; D014168:Transferrin; D012710:Serum Albumin, Bovine