Confirmatory evidence from 18S rRNA gene analysis for in vivo development of propamidine resistance in a temporal series of Acanthamoeba ocular isolates from a patient.
DNA sequences of three 18S rRNA gene alleles present in trophozoites obtained before and after therapy for Acanthamoeba keratitis substantiate a previous report that the infection was due to a single Acanthamoeba strain. Thus, the possibility that propamidine resistance which developed during therapy was due to a mixed infection was ruled out.
Ledee
D R
DR
Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA.
Seal
D V
DV
Byers
T J
TJ
D000048:Acanthamoeba / Q000187:drug effects / Q000235:genetics*; D015823:Acanthamoeba Keratitis / Q000188:drug therapy* / Q000821:virology; D000563:Amebicides / Q000627:therapeutic use*; D000818:Animals; D001550:Benzamidines / Q000627:therapeutic use*; D004275:DNA, Ribosomal / Q000737:chemistry; D004351:Drug Resistance; D006801:Humans; D012337:RNA, Ribosomal, 18S / Q000235:genetics*
D000563:Amebicides; D001550:Benzamidines; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; C005555:propamidine