Lipid peroxidation after cold storage and normothermic reperfusion: the effect of trimetazidine.
Ischemia reperfusion injury is still a leading cause of early graft dysfunction after transplantation. Trimetazidine (TMZ) has been postulated to be protective against renal damage from oxygen free radicals. The aim of this study was to assess the effect TMZ during cold storage (CS) and normothermic reperfusion in an isolated perfused pig kidney model. Three groups were studied: control group, immediately perfused (G0), 48 h CS in Euro-Collins solution (G1), and 48 h CS in Euro-Collins solution plus TMZ (G2). Glomerular filtration rate (GFR) and fractional sodium reabsorption (FRNa+) were calculated during reperfusion from urine and perfusate samples. Lipid peroxidation was determined by the renal tissue level of Schiff bases (SB) and malondialdehyde (MDA) after reperfusion. A histological evaluation was performed after reperfusion. Renal function was significantly improved and lipid peroxidation reduced after preservation in Euro-Collins solution plus TMZ. Functional data were closely related to histological damage. In conclusion, TMZ is a useful protective agent against renal damage induced by CS.
Institut National de Recherche Agronomique, Le Magneraud, Poitiers, Surgères, France.
T
T
Hauet
H
H
Baumert
D
D
Mothes
T
T
Germonville
J C
JC
Caritez
M
M
Carretier
F
F
Journe
M
M
Eugene
J P
JP
Tillement
D000818:Animals; D003080:Cold Temperature; D015227:Lipid Peroxidation; D015424:Reperfusion; D015427:Reperfusion Injury / Q000517:prevention & control*; D013552:Swine; D014292:Trimetazidine / Q000627:therapeutic use*; D014665:Vasodilator Agents / Q000627:therapeutic use*
D014665:Vasodilator Agents; D014292:Trimetazidine