The effect of curdlan sulphate on development in vitro of Plasmodium falciparum.
Sulphated glycoconjugates have been reported to inhibit malarial merozoite invasion and interfere with rosetting and adhesion. Curdlan sulphate, a sulphated glycoconjugate with a favourable toxicity profile, exhibits antimalarial activity in vitro. The aim of this study was to characterize the antimalarial activity of curdlan and investigate its effect on adhesion. The antimalarial activity of curdlan at different points in the intraerythrocytic developmental cycle was investigated using morphological observation and radiolabelled hypoxanthine uptake as indices of parasite growth. Effects on adhesion were investigated using a platelet model. Curdlan suphate had no effect on the ability of the parasite to develop through the intraerythrocytic cycle. Inhibition of invasion was dependent on the drug being present at the time of invasion. Curdlan did not interfere with the ability of the parasite to adhere to the C36 receptor in the platelet model. In conclusion, the low toxicity of curdlan and its marked anti-invasion activity on merozoites make curdlan a potential auxiliary treatment for severe malaria.
Department of Experimental and Clinical Pharmacology, University of the Witwatersrand, Parktown, South Africa.
S G
SG
Evans
D
D
Morrison
Y
Y
Kaneko
I
I
Havlik
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D004912:Erythrocytes / Q000469:parasitology*; D005936:Glucans / Q000494:pharmacology*; D006801:Humans; D008018:Life Cycle Stages; D016778:Malaria, Falciparum / Q000188:drug therapy; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000254:growth & development; D047071:beta-Glucans
D000962:Antimalarials; D005936:Glucans; D047071:beta-Glucans; C038459:curdlan