Establishment of ameloblastoma cell line, AM-1.
Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.
Second Department of Oral Anatomy and Cell Biology, Kyushu Dental College, Kitakyushu, Japan.
H
H
Harada
T
T
Mitsuyasu
N
N
Nakamura
Y
Y
Higuchi
K
K
Toyoshima
A
A
Taniguchi
S
S
Yasumoto
D000564:Ameloblastoma / Q000235:genetics / Q000473:pathology* / Q000821:virology; D017209:Apoptosis; D002455:Cell Division; D002999:Clone Cells / Q000473:pathology; D003094:Collagen; D003470:Culture Media; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness; D027383:Papillomaviridae / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D014162:Transfection / Q000235:genetics; D014407:Tumor Cells, Cultured
D003470:Culture Media; D019253:Proto-Oncogene Proteins c-bcl-2; D007633:Keratins; D003094:Collagen