Mrp1 multidrug resistance-associated protein and P-glycoprotein expression in rat brain microvessel endothelial cells.
Two membrane glycoproteins acting as energy-dependent efflux pumps, mdr-encoded P-glycoprotein (P-gp) and the more recently described multidrug resistance-associated protein (MRP), are known to confer cellular resistance to many cytotoxic hydrophobic drugs. In the brain, P-gp has been shown to be expressed specifically in the capillary endothelial cells forming the blood-brain barrier, but localization of MRP has not been well characterized yet. Using RT-PCR and immunoblot analysis, we have compared the expression of P-gp and Mrp1 in homogenates, isolated capillaries, primary cultured endothelial cells, and RBE4 immortalized endothelial cells from rat brain. Whereas the mdr1a P-gp-encoding mRNA was specifically detected in brain microvessels and mdr1b mRNA in brain parenchyma, mrp1 mRNA was present both in microvessels and in parenchyma. However, Mrp1 was weakly expressed in microvessels. Mrp1 expression was higher in brain parenchyma, as well as in primary cultured brain endothelial cells and in immortalized RBE4 cells. This Mrp1 overexpression in cultured brain endothelial cells was less pronounced when the cells were cocultured with astrocytes. A low Mrp activity could be demonstrated in the endothelial cell primary monocultures, because the intracellular [3H]vincristine accumulation was increased by several MRP modulators. No Mrp activity was found in the cocultures or in the RBE4 cells. We suggest that in rat brain, Mrp1, unlike P-gp, is not predominantly expressed in the blood-brain barrier endothelial cells and that Mrp1 and the mdr1b P-gp isoform may be present in other cerebral cells.
Regina
A
A
INSERM U26, Unité de Neuro-Pharmaco-Nutrition, Hôpital F. Widal, Paris, France.
Koman
A
A
Piciotti
M
M
El Hafny
B
B
Center
M S
MS
Bergmann
R
R
Couraud
P O
PO
Roux
F
F
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000493:pharmacokinetics; D001253:Astrocytes / Q000166:cytology / Q000502:physiology; D001553:Benzbromarone / Q000494:pharmacology; D001921:Brain / Q000098:blood supply*; D002196:Capillaries / Q000737:chemistry / Q000502:physiology; D002273:Carcinogens / Q000494:pharmacology; D002461:Cell Line, Transformed / Q000737:chemistry / Q000502:physiology; D003078:Colchicine / Q000493:pharmacokinetics; D016572:Cyclosporine / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000032:analysis / Q000037:antagonists & inhibitors / Q000235:genetics*; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000166:cytology / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D019833:Genistein / Q000494:pharmacology; D006074:Gout Suppressants / Q000493:pharmacokinetics; D015151:Immunoblotting; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D000074081:MutS Homolog 3 Protein; D016133:Polymerase Chain Reaction; D011339:Probenecid / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013442:Sulfinpyrazone / Q000494:pharmacology; D014528:Uricosuric Agents / Q000494:pharmacology; D014747:Vinblastine / Q000493:pharmacokinetics; D014750:Vincristine / Q000493:pharmacokinetics
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000972:Antineoplastic Agents, Phytogenic; D001553:Benzbromarone; D002273:Carcinogens; D003078:Colchicine; D016572:Cyclosporine; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D019833:Genistein; D006074:Gout Suppressants; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D000074081:MutS Homolog 3 Protein; D011339:Probenecid; D012333:RNA, Messenger; D013442:Sulfinpyrazone; D014528:Uricosuric Agents; D014747:Vinblastine; D014750:Vincristine; C078444:MSH3 protein, human; C421109:multidrug resistance-associated protein 1