A zinc finger domain gene in the lizard, Calotes versicolor, shows extensive homology with the mammalian ZFX and is expressed embryonically.
A 590-bp long zinc finger domain DNA fragment has been isolated by polymerase chain reaction from the lizard, Calotes versicolor, employing the primers used for amplifying the zinc finger domain of the human Y-chromosomal gene, ZFY. Cloned in pUC18, the fragment, called CvZfa, was sequenced and its expression during development was studied. At the nucleotide and amino acid level CvZfa shows respectively 83% and 90% identity with the human ZFY, but its extent of homology is greater with the ZFX of human (86% at nucleotide and 92% at amino acid level) and the ZFY-like genes of turtle and chick. Similarly its homology with the mouse Zfx and Zfa is much greater than that with Zfy-1 and Zfy-2. It appears that the mammalian ZFX (Zfx) evolved from reptilian ancestors with a considerable degree of conservation, but the ZFX to ZFY divergence within the class mammalia was more rapid. The CvZfa transcripts were seen in all the embryonic stages from which RNA was analysed. The whole mount in situ hybridization with the posteriorly placed mesonephros and the gonadal primordia of 10 to 25 day old embryos showed signal selectively in mesonephros of the 20 and 25 day embryos. There was no signal in the genital ridge. Thus CvZfa may not have a direct role in gonadogenesis of C. versicolor, but the possibility of its inductive role in the formation of adreno-gonadal axis through mesonephros cannot be discounted.
Department of Zoology, Banaras Hindu University, Varanasi, India.
S
S
Ganesh
B
B
Choudhary
R
R
Raman
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004247:DNA; D004268:DNA-Binding Proteins / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008116:Lizards / Q000196:embryology / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014157:Transcription Factors; D016335:Zinc Fingers / Q000235:genetics*
D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C082035:Zfa protein, mouse; C062076:zinc finger protein, X-linked; D004247:DNA