A diffusible resistance factor(s) in spontaneous mitomycin resistant mammalian cancer cells.
We developed a Mitomycin C (MMC)-resistant mouse breast cancer cell subline, R-FM3A, from FM3A parent cells(W-FM3A) by continuous exposure to a concentration of 0.01 microgram/ml MMC for over two months. R-FM3A cells were resistant to MMC and decreased in response to the combined effects of ionizing irradiation and MMC. The resistance to MMC and ionizing irradiation was mediated by a cell-free medium, in which R-FM3A cells were cultured for 12 hours without MMC (Re-medium). The cell cycles measured by means of a flow cytometer were not influenced by the Re-medium when W-FM3A cells were treated by MMC. But the cell cycles measured by means of a flow cytometer were influenced by the Re-medium when W-FM3A cells were irradiated at a dose of 4 Gy.
Department of Radiology, Nagoya University School of Medicine, Japan.
Z G
ZG
Wan
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H
Kobayashi
A
A
Hayakawa
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T
Ishigaki
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D017077:Culture Media, Conditioned; D004058:Diffusion; D004351:Drug Resistance / Q000502:physiology; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000532:radiotherapy; D051379:Mice; D016685:Mitomycin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000903:Antibiotics, Antineoplastic; D017077:Culture Media, Conditioned; D016685:Mitomycin