Quantitative recovery of Man9GlcNAc2Asn derivatives from concanavalin A.
Some larger high-mannose-type oligosaccharides bind very tightly to concanavalin A and are difficult to elute. We present conditions that permit the complete elution of compounds containing high-mannose type oligosaccharides from a popular concanavalin A-Sepharose that is commercially available. Europium-labeled Man9GlcNAc2Asn (N. Kawasaki and Y.C. Lee, Anal. Biochem., 250 (1997) 260-262), soybean agglutinin, and Eu(III)-labeled soybean agglutinin bound to concanavalin A-Sepharose were completely eluted with 1 M methyl alpha-D-mannopyranoside by allowing the column to stand in elution buffer, permitting the oligosaccharide or glycoprotein to slowly dissociate from the column.
Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA.
I L
IL
Deras
N
N
Kawasaki
Y C
YC
Lee
D001216:Asparagine / Q000737:chemistry; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry*; D005063:Europium / Q000737:chemistry; D006020:Glycopeptides / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry*
D006020:Glycopeptides; D009844:Oligosaccharides; D003208:Concanavalin A; D005063:Europium; D001216:Asparagine; D008358:Mannose