Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells.
We previously showed in a primary culture of rat testicular cells that spermatogenic cells specifically bind to somatic Sertoli cells and that this interaction is needed for spermatogenic cells to differentiate in vitro. Adopting an expression cloning procedure, we here isolated a cDNA coding for a spermatogenic cell protein whose expression gave a cultured cell line the ability to bind to Sertoli cells. The protein, 243 amino acids with a putative N-terminal signal peptide and a C-terminal Cys-rich region, turned out to be the rat homologue of a testicular protein called Tpx-1 whose function had yet to be determined. A polyclonal antibody raised against bacterially expressed Tpx-1 significantly inhibited the binding of spermatogenic cells to Sertoli cells. The above results indicated that Tpx-1 is a testicular cell adhesion molecule responsible for the specific interaction between spermatogenic and Sertoli cells.
Graduate School for Natural Science and Technology, Kanazawa University, Japan.
T
T
Maeda
M
M
Sakashita
Y
Y
Ohba
Y
Y
Nakanishi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D002478:Cells, Cultured; D003001:Cloning, Molecular; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D006801:Humans; D019169:Jurkat Cells; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism*; D013737:Testis / Q000166:cytology*
C062790:CRISP2 protein, rat; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins