Gastric ulcer formation in cold-stressed mice related to a central calcium-dependent-dopamine synthesizing system.
Stress ulceration of the stomach in mice was investigated from the aspect of the calcium/calmodulin-dependent dopamine synthesizing system in the brain. Cold stress was induced in mice by restraining them at 4 degrees C. Serum and brain calcium levels were increased by cold stress, and an increased brain calcium level was found to enhance dopamine synthesis and a successively increased brain dopamine level induced gastric ulcer formation. Development of gastric ulcers elicited by cold stress was significantly decreased by i.p. pretreatment with EDTA (1 micromol/mouse, 1 h before restraint) or alpha-methyltyrosine (a tyrosine hydroxylase inhibitor, 100 mg/kg, 24 h before restraint), and was further significantly increased by pretreatment with CaCl2 (40 micromol/kg, 1 h before restraint). These findings suggest that the development of gastric ulcers in cold-stressed mice may be linked with the enhancement of calcium/calmodulin-dependent catecholamine synthesis in the brain.
Department of Pediatrics, Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan. dsutoo@md.tsukuba.ac.jp
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D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D003080:Cold Temperature; D004298:Dopamine / Q000096:biosynthesis*; D004492:Edetic Acid / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D013276:Stomach Ulcer / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D013312:Stress, Physiological / Q000097:blood / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors; D019805:alpha-Methyltyrosine / Q000494:pharmacology
D004791:Enzyme Inhibitors; D019805:alpha-Methyltyrosine; D004492:Edetic Acid; D014446:Tyrosine 3-Monooxygenase; D002118:Calcium; D004298:Dopamine