Expression of platelet-derived endothelial cell growth factor and its mRNA in uterine endometrium during the menstrual cycle.
Steroid hormones, e.g. progesterone and oestradiol, may be responsible for the production and expression of a variety of angiogenic growth factors present in endometrial tissue. The expression of platelet-derived endothelial cell growth factor (PD-ECGF) in neovascularization after regression of the microvessels in the endometrium was examined. PD-ECGF protein expression in the endometrium during the menstrual cycle was determined by a sandwich enzyme immunoassay. Transcription levels of PD-ECGF were measured by a quantitative reverse transcription-polymerase chain reaction (RT-PCR) Southern blot technique. The data show that levels of PD-ECGF protein and mRNA in uterine endometrium did not alter during the proliferative phase prior to ovulation. During the midcycle phase a sharp transient fall in mRNA levels accompanied by a gradual drop in protein levels was observed. After ovulation transcription of PD-ECGF recovered with a sharp increase in mRNA levels which persisted during the ovulatory phase. PD-ECGF protein levels were temporarily low after ovulation, but increased remarkably through the late secretory phase. PD-ECGF expression in the endometrium seems to be inversely correlated with oestradiol concentrations during the menstrual cycle.
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
J
J
Fujimoto
S
S
Ichigo
H
H
Sakaguchi
R
R
Hirose
T
T
Tamaya
D000328:Adult; D015139:Blotting, Southern; D004717:Endometrium / Q000098:blood supply / Q000378:metabolism*; D004958:Estradiol / Q000502:physiology; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008597:Menstrual Cycle / Q000235:genetics*; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000235:genetics; D010060:Ovulation / Q000235:genetics; D016133:Polymerase Chain Reaction; D011374:Progesterone / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D013939:Thymidine Phosphorylase / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic
D012333:RNA, Messenger; D011374:Progesterone; D004958:Estradiol; D013939:Thymidine Phosphorylase