Presence of a fatty acid-binding protein in the armadillo Harderian gland.
A fatty acid-binding protein from the cytosolic fraction of the armadillo Chaetophractus villosus Harderian gland was purified to homogeneity by a procedure based on gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein has an apparent molecular mass of 14 kDa. N-terminal sequence analysis showed that the protein has a blocked N-terminus. For internal amino acid sequencing, the protein was digested in-gel and the resulting peptides were fractionated by reverse-phase high performance liquid chromatography and subjected to automated Edman degradation. Partial amino acid sequencing suggests that it belongs to the heart type. Moreover, it cross-reacted with anti-serum to rat heart fatty acid-binding protein but not with rat intestinal and liver anti-sera. A very slow cross-reaction was also found with anti-serum to rat ALBP. This is the first time that a fatty acid-binding protein has been reported in a Harderian gland.
Instituto de Química y Fisicoquímica Biológicas (IQUIFIB)-CONICET-UBA, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
B M
BM
Cavagnari
O L
OL
Córdoba
J H
JH
Veerkamp
J A
JA
Santomé
J M
JM
Affanni
D000595:Amino Acid Sequence; D000818:Animals; D001135:Armadillos; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D006243:Harderian Gland / Q000378:metabolism*; D008969:Molecular Sequence Data; D018996:Myelin P2 Protein / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D016415:Sequence Alignment
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins