Laminin, a major basement membrane component of the blood vessel, as a negative regulator of osteoclastogenesis.
Laminin, the major basement membrane glycoprotein of the blood vessel, inducing many cellular responses, inhibited the differentiation of osteoclasts in a rat bone marrow culture system when immobilized on the surface of the culture wells, showing that laminin acts as a negative regulator of osteoclast differentiation in a nonsolubilized form. Laminin inhibited the process of preosteoclast formation from early progenitor cells in bone marrow. This laminin-mediated inhibition of osteoclastogenesis was blocked by the addition of laminin fragment YIGSR, indicating that the inhibitory effect of laminin was mediated via laminin receptors. This finding suggests a significant role of basement membrane laminin of the blood vessels as a negative regulator of osteoclastogenesis.
Second Department of Oral Anatomy, Faculty of Dentistry, Kyushu University, Fukuoka, Japan.
T
T
Kukita
K
K
Hata
A
A
Kukita
T
T
Iijima
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001808:Blood Vessels / Q000737:chemistry; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015726:Giant Cells / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D007797:Laminin / Q000494:pharmacology*; D051379:Mice; D009842:Oligopeptides / Q000494:pharmacology; D010010:Osteoclasts / Q000166:cytology* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071681:Tartrate-Resistant Acid Phosphatase; D013977:Tibia / Q000166:cytology / Q000187:drug effects
D007527:Isoenzymes; D007797:Laminin; D009842:Oligopeptides; C081571:tyrosyl-isoleucyl-glycyl-seryl-arginine; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase