Effect of Chinese herbal medicines and disodium cromoglycate on IgE-dependent histamine release from mouse cultured mast cells.
We examined the effect of anti-allergic Chinese herbal medicines such as Ma-Xing-Gan-Shi-Tang (MXGST) and Xiao-Feng-San (XFS), and a mast cell stabilizer, disodium cromoglycate (DSCG) on histamine release from mouse cultured mast cells. The mast cells (ILMCMC) were obtained by culturing mouse bone marrow cells for 3-6 weeks in the presence of IL-3. Some of the cells (FMCMC) were further cultured with a fibroblast cell line, 3T3 for 3 weeks. FMCMC had safranin-positive granules and released histamine in response to compound 48/80, whereas ILMCMC failed to do so. MXGST and XFS at 4-40 micrograms/ml inhibited IgE-dependent histamine release from ILMCMC but not from FMCMC. On the contrary, DSCG at 10(-4) M inhibited histamine release from FMCMC but not from ILMCMC. Chinese herbal medicines and DSCG may act on different types of mast cells.
Shichijo
K
K
Department of Pediatrics, Jikei University School of Medicine, Tokyo, Japan.
Saito
H
H
D000818:Animals; D018927:Anti-Asthmatic Agents / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D004205:Cromolyn Sodium / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA
D018927:Anti-Asthmatic Agents; D004365:Drugs, Chinese Herbal; D007073:Immunoglobulin E; D004205:Cromolyn Sodium