Expression of Panulirus shaker potassium channel splice variants.
In Drosophila shaker voltage-dependent potassium channels, alternative splicing at the amino and carboxy termini produces currents with different electrophysiological characteristics. We have cloned alternatively spliced forms of shaker from the spiny lobster Panulirus interruptus. Alternative exons were found at three sites of the gene; eight different 5' exons, two alternative exons encoding the pore-forming P region, and an alternative 3' exon. Two of the different amino terminal splice forms were expressed with two alternatively spliced pore forms to produce channels with markedly different characteristics. One of the amino termini produced a channel with transient characteristics while the other produced a delayed rectifier-type channel. The effects of alternative exons at the amino terminus and in the P region appear to be additive. Our results provide new information on the structural requirements for rapid N-type inactivation.
Section of Neurobiology and Behavior, Cornell University, Ithaca, NY 14853, USA.
M
M
Kim
D J
DJ
Baro
C C
CC
Lanning
M
M
Doshi
H S
HS
Moskowitz
J
J
Farnham
R M
RM
Harris-Warrick
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000502:physiology*; D015221:Potassium Channels / Q000502:physiology*
D015221:Potassium Channels