One base change is sufficient for host range conversion of murine leukemia virus from B to NB tropism.
Comparison of the whole p30 (CA) sequence between B-tropic WN1802B murine leukemia virus and its NB-tropic derivative revealed a single base change at the 313th nucleotide. We constructed hybrid proviruses differing only at the 313th nucleotide of p30 along the whole genome, and examined the host range of the produced viruses. The single point mutation in p30 was found sufficient for the B to NB tropism conversion.
Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.
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K
Doi
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A
Kawana
A
A
Iwamoto
H
H
Yoshikura
T
T
Odawara
D000818:Animals; D002213:Capsid / Q000235:genetics*; D003001:Cloning, Molecular; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D017354:Point Mutation; D011533:Proviruses / Q000235:genetics