Contractile properties and histochemical characteristics of the rat diaphragm after prolonged triamcinolone treatment and nutritional deprivation.
The influence of decreased muscle mass and reduced food intake on diaphragm structure and contractility in male Wistar rats was determined after triamcinolone acetate treatment (TR: 0.5 mg per kg per day for 4 weeks) and two degrees of undernutrition (PW: pair-weight, which resulted in a similar (41%) reduction of body weight as TR; PF: pair-fed, which resulted in a moderate (13%) reduction of body weight) and a free-fed control group (FF, with an increase (9%) in body weight). energy intake of TR decreased, but based on daily measurements of food intake and body weight, energy expenditure of the TR rats was increased compared with the other groups. Body (BW) and muscle weights were reduced in proportion to the extent of undernutrition in the nutritionally deprived rates (i.e. BW and diaphragm weight of PF animals were reduced 215 and 16% respectively compared with FF, v. a. 48% and 41% reduction in the PW group). Triamcinolone-induced atrophy was limited to type II fibres (30% of type IIa and 45% of type IIx/b, p < 0.05), while severe chronic undernutrition (PW) induced a generalized fibre type atrophy in the diaphragm (23% type I, 38% type IIa and 49% type IIx/b, p < 0.05), and moderate undernutrition (PF) caused only significant type IIa atrophy (20%, p < 0.05). A leftward shift of the diaphragmatic tension-frequency relationship and a decreased fatiguability of the TR and PW bundles were observed (p < 0.01), while the PF bundles were not significantly different compared with FF. These results suggest that triamcinolone and severe undernutrition cause similar alterations in in vitro contractility of the diaphragm. The effects of triamcinolone treatment on diaphragm structure may be partly explained by the reduced food intake, but the atrophy pattern induced by severe undernutrition (PW) was different.
Department of Pulmonology, Maastricht University, The Netherlands.
E
E
Koerts-De Lang
A M
AM
Schols
E F
EF
Wouters
G
G
Gayan-Ramirez
M
M
Decramer
D000367:Age Factors; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001284:Atrophy; D003964:Diaphragm / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology*; D005508:Food Deprivation / Q000502:physiology*; D006651:Histocytochemistry; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000187:drug effects; D018657:Muscle Fibers, Slow-Twitch / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014221:Triamcinolone / Q000494:pharmacology*
D000893:Anti-Inflammatory Agents; D014221:Triamcinolone