Translocation of protein kinase Cgamma occurs during the early phase of acquisition of food rewarded spatial learning.
This study describes the translocation of the brain specific protein kinase C gamma isoenzyme (PKCgamma) in the hippocampus during food rewarded spatial learning. The holeboard test was used for spatial orientation, and immunoblot analysis was used for assessment of PKCgamma in cytosolic, membrane-inserted and membrane-associated fractions. Membrane-associated PKCgamma was increased during early acquisition of spatial learning, but not in a later phase of training. This transient and apparently temporary intracellular PKCgamma translocation was only observed in the posterior but not in the anterior hippocampus, and was only detected within 10 min after termination of the learning trial. This study supports the idea that PKCgamma is significantly involved in the biochemical events underlying learning and memory, notably during the period of novel information processing. The results further promote the hypothesis that the hippocampus is specifically involved in temporal information processing, which requires the engagement of PKCgamma.
Douma
B R
BR
Department of Animal Physiology, University of Groningen, Haren, The Netherlands. B.R.K.Douma@biol.rug.nl
Van der Zee
E A
EA
Luiten
P G
PG
D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D004193:Discrimination Learning / Q000502:physiology*; D006624:Hippocampus / Q000201:enzymology*; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D008568:Memory / Q000502:physiology*; D009949:Orientation / Q000502:physiology*; D011493:Protein Kinase C / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013028:Space Perception / Q000502:physiology; D013997:Time Factors
D007527:Isoenzymes; D008565:Membrane Proteins; D011493:Protein Kinase C