Parallel radiation hybrid mapping: a powerful tool for high-resolution genomic comparison.
Comparative gene mapping in mammals typically involves identification of segments of conserved synteny in diverse genomes. The development of maps that permit comparison of gene order within conserved synteny has not advanced beyond the mouse map that takes advantage of linkage analysis in interspecific backcrosses. Radiation hybrid (RH) mapping provides a powerful tool for determining order of genes in genomes for which gene-based linkage mapping is impractical. Comparative RH mapping of 24 orthologous genes in this study revealed internal structural rearrangements between human chromosome 17 (HSA17) and bovine chromosome 19 (BTA19), two chromosomes known previously to be conserved completely and exclusively at level of synteny. Only six of the 24 genes had been previously ordered on the human G3 RH map. The use of the G3 panel to map the other 18, however, produced parallel RH maps for comparison of gene order at a resolution of <5 Mb on the bovine linkage map and from 1 to 3 Mb in the human physical map.
Yang
Y P
YP
Department of Veterinary Pathobiology and Center for Animal Genetics, Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843-4467 USA.
Womack
J E
JE
D000818:Animals; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods*; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D005819:Genetic Markers; D015698:Genomic Library; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism* / Q000528:radiation effects; D014178:Translocation, Genetic
D005819:Genetic Markers