Murine Stim1 maps to distal chromosome 7 and is not imprinted.
STIM1 (GOK) maps to a region of human Chromosome (Chr) 11p15.5 that is implicated in several embryonal tumors, and some evidence indicates that STIM1 may have a growth suppressor role in rhabdomyosarcoma. In this study we have mapped the murine homolog, Stim1, to the same position as Hbb on distal mouse Chr 7. This region is separated by 20 cM from the region of distal Chr 7 that contains Igf2, H19, and other imprinted genes. Using strain-specific polymorphisms, we have shown that Stim1 is expressed from both parental alleles in fetal and neonatal mouse tissues. Similar analyses of human Wilms' tumor and normal kidney tissues demonstrated biallelic expression of STIM1 in the majority of samples. These data demonstrate that Stim1 expression is not regulated by genomic imprinting in either mouse or human tissues. Thus, if STIM1 is a tumor suppressor at 11p15.5, loss of expression is not due to imprinting effects.
Overall
M L
ML
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Victoria 3052, Australia.
Parker
N J
NJ
Scarcella
D L
DL
Smith
P J
PJ
Dziadek
M
M
D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D005260:Female; D005333:Fetus; D005819:Genetic Markers; D018392:Genomic Imprinting; D006801:Humans; D007668:Kidney / Q000378:metabolism; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009115:Muridae; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D000071737:Stromal Interaction Molecule 1; D009396:Wilms Tumor / Q000235:genetics / Q000378:metabolism
D005819:Genetic Markers; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1