Time dependent increase of differential monocyte count on the Sysmex NE-8000.
During storage of whole blood samples the results of white blood cell counts on a Sysmex NE-8000 Hematology Analyser showed an apparent shift from the granulocytes towards monocytes. The effects of various storage conditions were investigated by incubating blood samples at different temperatures and time intervals. Granulocyte activation and degranulation during storage resulted in a false elevation of the automated monocyte differential counts. The phenomenon occurs at temperatures beyond ambient room temperature.
Department of Clinical Chemistry, Haematology and Immunology, Medical Centre Alkmaar, The Netherlands.
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Bartels
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Schoorl
D016477:Artifacts; D001331:Automation; D001793:Blood Preservation; D003594:Cytoplasmic Granules / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005189:False Positive Reactions; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation*; D009000:Monocytes; D013696:Temperature; D013997:Time Factors
D004492:Edetic Acid