Characterization of a newly established human chondrosarcoma cell line, CS-OKB.
A clonal cell line, CS-OKB, was derived from a human chondrosarcoma and characterized by cytogenetic study, immunocytochemical staining, and reverse transcriptase polymerase chain reaction (RT-PCR). Chromosomal abnormalities characteristic of malignant cartilaginous neoplasms were identified. CS-OKB cells were intensely stained with anti-type II collagen and anti-keratan sulphate antibodies. RT-PCR indicated that CS-OKB transcribes cartilage-specific genes such as type II, X procollagen, and aggrecan. This human chondrosarcoma cell line is stable and expresses well-differentiated chondrocyte-specific genes. It synthesizes well-differentiated chondrocyte-specific molecules in uncoated plastic dishes. CS-OKB may be useful for studies of human chondrocytes and in characterizing human chondrosarcomas.
Chano
T
T
Department of Orthopaedic Surgery, Shiga University of Medical Science, Ohtsu, Japan.
Okabe
H
H
Saeki
Y
Y
Ishizawa
M
M
Matsumoto
K
K
Hukuda
S
S
D000368:Aged; D000369:Aged, 80 and over; D053674:Aggrecans; D048429:Cell Size; D002813:Chondrosarcoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D003094:Collagen / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D006801:Humans; D007150:Immunohistochemistry; D007621:Karyotyping; D007632:Keratan Sulfate / Q000378:metabolism; D037181:Lectins, C-Type; D008297:Male; D016133:Polymerase Chain Reaction; D011347:Procollagen / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000166:cytology* / Q000378:metabolism
D053674:Aggrecans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011347:Procollagen; D011509:Proteoglycans; D012333:RNA, Messenger; D003094:Collagen; D007632:Keratan Sulfate