Identification and cloning of two novel allergens from the lipophilic yeast, Malassezia furfur.
Two novel allergens, designated Mal f 2 and Mal f 3 according to the WHO/IUIS Allergen Nomenclature Subcommittee recommendation, were isolated from the lipophilic yeast Malassezia furfur cell extracts and the genes coding for those were cloned. Mal f 2 and Mal f 3 had apparent molecular weights of 21 kDa and 20 kDa, respectively, on SDS-PAGE under reducing conditions. The identified cDNA clone of Mal f 2 encoded an open reading frame of 177 amino acid residues. Fifty-one percent identity was found between the Mal f 2 and Mal f 3 sequences. Comparison of the Mal f 2 and Mal f 3 sequences with known protein sequences revealed that they had sequence homology with two peroxisomal membrane proteins of Candida boidinii and an Aspergillus fumigatus allergen, Asp f 3. In RAST, both Mal f 2- and Mal f 3-specific IgE antibodies could be detected in approximately 70 % of sera from M. furfur sensitized patients with atopic dermatitis.
Clinical Research Center for Allergy and Rheumatology, National Sagamihara Hospital, 18-1 Sakuradai, Kanagawa, Sagamihara, 228-8522, Japan.
H
H
Yasueda
T
T
Hashida-Okado
A
A
Saito
K
K
Uchida
M
M
Kuroda
Y
Y
Onishi
K
K
Takahashi
H
H
Yamaguchi
K
K
Takesako
K
K
Akiyama
D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D001230:Aspergillus / Q000737:chemistry; D001483:Base Sequence; D002175:Candida / Q000737:chemistry; D003001:Cloning, Molecular; D003876:Dermatitis, Atopic / Q000276:immunology; D005656:Fungal Proteins / Q000737:chemistry*; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008290:Malassezia / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D008830:Microbodies / Q000737:chemistry; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000485:Allergens; D005656:Fungal Proteins; C113870:Mal f 2 allergen; C113871:Mal f 3 allergen; D008565:Membrane Proteins; D007073:Immunoglobulin E