Direct identification of Coxiella burnetii plasmids in human sera by nested PCR.
Nested PCR assays were used for the direct identification of Coxiella burnetii plasmids in human sera. A total of 81 serum samples from 81 patients with Q fever were tested by nested PCR with four sets of primers. The first set of primers was used to detect the genomic sequences. The second set of primers was used to detect the conserved sequences of the plasmids. Another two sets of primers were used to identify the QpH1 and QpRS plasmids. QpH1 and QpRS plasmid-specific sequences were identified in 40 (49.4%) and 24 (29.6%) of the serum samples, respectively. Both of the QpH1 and QpRS plasmid-specific sequences were detected in 5 (8.6%) of the serum samples but were not found in 12 (20.7%) of the serum samples. Furthermore, all of the 23 acute-phase serum samples were positive for the QpH1 plasmid and negative for the QpRS plasmid. Nested PCR with plasmid-specific primers appears to be a useful method for the direct typing of C. burnetii plasmids in human sera.
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
G Q
GQ
Zhang
A
A
Hotta
M
M
Mizutani
T
T
Ho
T
T
Yamaguchi
H
H
Fukushi
K
K
Hirai
D000208:Acute Disease; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D016997:Coxiella burnetii / Q000235:genetics* / Q000302:isolation & purification*; D017931:DNA Primers; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D008297:Male; D010957:Plasmids / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011778:Q Fever / Q000175:diagnosis* / Q000382:microbiology; D012680:Sensitivity and Specificity
D017931:DNA Primers