Chromatographic removal of endotoxin from hemoglobin preparations. Effects of solution conditions on endotoxin removal efficiency and protein recovery.
In this work, affinity chromatography was used to remove endotoxin from human hemoglobin preparations with a Sterogene Acticlean Etox column. The effects of solution conditions on endotoxin removal efficiency and protein recovery have been investigated. It has been found that cations Na(+) or Ca(2+) reduced endotoxin removal efficiency from 73% (sample prepared with endotoxin-free water) to 31% (sample prepared with 0.15 M NaCl, ionic strength, I = 0.15 M), and from 73% sample prepared with endotoxin-free water) to 9% (sample prepared with 0.05 M CaCl2, ionic strength, I = 0.15 M). It has also been found that the protein recovery was increased from 90% to 99%, respectively.
Department of Chemical Engineering, Chemistry and Environmental Science, New Jersey Institute of Technology, University Heights, Newark 07102, USA.
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Kang
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Luo
D002122:Calcium Chloride; D002846:Chromatography, Affinity; D004731:Endotoxins / Q000097:blood*; D006441:Hemoglobin A / Q000302:isolation & purification; D006454:Hemoglobins / Q000302:isolation & purification*; D006801:Humans; D008070:Lipopolysaccharides; D009994:Osmolar Concentration; D012965:Sodium Chloride; D012996:Solutions
D004731:Endotoxins; D006454:Hemoglobins; D008070:Lipopolysaccharides; D012996:Solutions; D012965:Sodium Chloride; C028881:hemoglobin A(0); D006441:Hemoglobin A; D002122:Calcium Chloride