In vitro cytotoxicity and teratogenicity of norethisterone and levonorgestrel released from hollow nylon monofilaments.
Direct delivery of progestogens to the uterus may be of use in the treatment of dysfunctional uterine bleeding and the sequelae of the menopause as it has the potential to overcome the problems of systemic administration. This study characterised the release of norethisterone and levonorgestrel into an aqueous medium from hollow nylon fibres with dimensions suitable for easy insertion through the post-menopausal cervix. Cell culture techniques were used to assess potential cytotoxic and teratogenic effects. The results demonstrated that the hollow fibres released norethisterone and levonorgestrel at mean rates of 0.5 and 0.6 micrograms/day over 14 days, respectively. There were indications, however, that both steroids were toxic to endometrial cells at concentrations of approximately 5 micrograms/ml, and both drugs showed signs of potential teratogenicity at 10 micrograms/ml. Delivery of the same doses of norethisterone using the hollow fibres reduced the effects on the endometrial and fetal cells. Delivery of levonorgestrel from the hollow fibres had no effect on the endometrial cell toxicity but potentiated the effects on the fetal cells. These results suggest that the hollow nylon fibres may be of use for the delivery of norethisterone to the uterus but that they are inappropriate for the delivery of levonorgestrel.
Department of Pharmacy, University of Brighton, Moulsecoomb, UK, snaser@chamran.ut.ac.ir
S N
SN
Ostad
J S
JS
Malhi
P R
PR
Gard
D000014:Abnormalities, Drug-Induced; D000818:Animals; D002478:Cells, Cultured; D003280:Contraceptives, Oral, Synthetic / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D005260:Female; D016912:Levonorgestrel / Q000008:administration & dosage* / Q000633:toxicity; D009640:Norethindrone / Q000008:administration & dosage* / Q000633:toxicity; D009757:Nylons; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D003280:Contraceptives, Oral, Synthetic; D009757:Nylons; D016912:Levonorgestrel; D009640:Norethindrone