Ovarian cancer gene therapy.
Retroviral-mediated delivery of BRCA1 gene therapy (LXN-BRCA1sv, a normal splice variant form of BRCA1) was tested extensively in mouse models. It was found to be effective in reducing tumor burden and to be minimally toxic. Twelve phase I clinical trial patients with recurrent or persistent epithelial ovarian cancer were treated with one to three cycles of intraperitoneal vector. There was minimal toxicity, four patients developed fevers (< 102.5 degrees F) and three had sterile peritonitis, which resolved within 48 hours. The vector was found to be fairly stable in some patients at 24 hours as well as transferred into and expressed in patient tissues. Stable disease was noticed in 8 of the 12 patients, suggesting that the peritoneal cavity may be an appropriate site for gene therapy.
Vanderbilt University Medical Center, Nashville, Tennessee, USA.
D L
DL
Tait
P S
PS
Obermiller
R A
RA
Jensen
J T
JT
Holt
D000818:Animals; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics / Q000628:therapy*