Production and properties of L-asparaginase from Mucor species associated with a marine sponge (Spirastrella sp.).
The fungus Mucor sp. isolated from the marine sponge Spirastrella sp. produced extracellular L-asparaginase. The maximum L-asparaginase activity was found at 50 degrees C. The activation and deactivation energies of the partially purified enzyme were 9.58 and 21.69 kcal mol-1, respectively. The enzyme activity was not affected by the addition of 4% (w/v) NaCl and different metal ions (Co+2, Fe+2, Mg+2, Ni+2, and Zn+2) at 25 mmol l-1 but was strongly inhibited by EDTA.
Mohapatra
B R
BR
Forest and Marine Product Division, Regional Research Laboratory, CSIR, Bhubaneswar, India.
Bapuji
M
M
Banerjee
U C
UC
D000818:Animals; D001215:Asparaginase / Q000737:chemistry* / Q000302:isolation & purification*; D002244:Carbon; D002413:Cations, Divalent; D002614:Chelating Agents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D009089:Mucor / Q000201:enzymology*; D009584:Nitrogen; D011161:Porifera / Q000382:microbiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D002413:Cations, Divalent; D002614:Chelating Agents; D012965:Sodium Chloride; D002244:Carbon; D004492:Edetic Acid; D001215:Asparaginase; D009584:Nitrogen