In situ hybridization of five loci to cattle chromosome 1.
The genes for pituitary-specific transcription factor (PIT1), propionyl coenzyme A carboxylase, beta-polypeptide (PCCB), transferrin (TF), trichohyalin (THH), and involucrin (IVL) were mapped to cattle chromosome 1 (BTA 1) by isotopic in situ hybridization. Two of the loci were mapped from cattle PCR products and three from human ATCC probes. PIT1 localized to segment 1q2; PCCB to 1q3; and TF, THH, and IVL to 1q4. These localizations agree with the homology previously shown between BTA 1 and human chromosome 3 (HSA 3). Some homology with HSA 1 has been established with the mapping of THH and IVL to BTA 1q4.
Department of Animal and Poultry Science, University of Saskatchewan, Saskatoon, Saskatchewan (Canada). schmutz@sask.usask.ca
S M
SM
Schmutz
J S
JS
Moker
T G
TG
Berryere
D000818:Animals; D002262:Carboxy-Lyases / Q000235:genetics; D002417:Cattle; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage; D006801:Humans; D017403:In Situ Hybridization; D007381:Intermediate Filament Proteins; D043586:Methylmalonyl-CoA Decarboxylase; D011498:Protein Precursors / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics; D014168:Transferrin / Q000235:genetics
D004268:DNA-Binding Proteins; D007381:Intermediate Filament Proteins; C494187:POU1F1 protein, human; D011498:Protein Precursors; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; D014168:Transferrin; C016159:trichohyalin; C032253:involucrin; D004247:DNA; D002262:Carboxy-Lyases; D043586:Methylmalonyl-CoA Decarboxylase