Quantitative receptor radioautography in the study of receptor-receptor interactions in the nucleus tractus solitarii.
The nucleus tractus solitarii (NTS) in the dorsomedial medulla comprises a wide range of neuropeptides and biogenic amines. Several of them are related to mechanisms of central blood pressure control. Angiotensin II (Ang II), neuropeptide Y (NPY) and noradrenaline (NA) are found in the NTS cells, as well as their receptors. Based on this observation we have evaluated the modulatory effect of these peptide receptors on alpha 2-adrenoceptors in the NTS. Using quantitative receptor radioautography, we observed that NPY and Ang II receptors decreased the affinity of alpha 2-adrenoceptors for their agonists in the NTS of the rat. Cardiovascular experiments agreed with the in vitro data. Coinjection of a threshold dose of Ang II or of the NPY agonists together with an ED50 dose of adrenergic agonists such as NA, adrenaline and clonidine counteracted the depressor effect produced by the alpha 2-agonist in the NTS. The results provide evidence for the existence of an antagonistic interaction between Ang II AT1 receptors and NPY receptor subtypes with the alpha 2-adrenoceptors in the NTS. This receptor interaction may reduce the transduction over the alpha 2-adrenoceptors which can be important in central cardiovascular regulation and in the development of hypertension.
Fior-Chadi
D R
DR
Departamento de Fisiologia, Universidade de São Paulo, Brasil. drfchadi@usp.br
Fuxe
K
K
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001345:Autoradiography; D001794:Blood Pressure / Q000502:physiology*; D003000:Clonidine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D006973:Hypertension / Q000503:physiopathology; D009478:Neuropeptide Y; D009638:Norepinephrine / Q000494:pharmacology; D051381:Rats; D011941:Receptors, Adrenergic / Q000502:physiology*; D011945:Receptors, Angiotensin / Q000502:physiology*; D017476:Receptors, Neuropeptide Y / Q000502:physiology*; D017552:Solitary Nucleus / Q000737:chemistry*
D009478:Neuropeptide Y; D011941:Receptors, Adrenergic; D011945:Receptors, Angiotensin; D017476:Receptors, Neuropeptide Y; D000804:Angiotensin II; D003000:Clonidine; D009638:Norepinephrine; D004837:Epinephrine