Two-dimensional electrophoresis of proteins in an immobilized pH 4-12 gradient.
For checking theoretical two-dimensional (2-D) maps derived from sequenced genomes, indicating that nonnegligible amounts of proteins up to pH 12 are to be expected, a wide-range immobilized pH 4-12 gradient was generated. Depending on the extraction method of sample preparation, proteins with pls up to pH 12 are detected in a single gel. Highly reproducible protein patterns focused to the steady state with round-shaped spots up to pH 12 are obtained with the standard protocol originally described in 1988 (Görg et al., Electrophoresis 1988, 9, 531-546).
Technical University of Munich, Department of Food Technology, Freising-Weihenstephan, Germany. angelika.gorg@lrz.tu-muenchen.de
A
A
Görg
G
G
Boguth
C
C
Obermaier
W
W
Weiss
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000032:analysis*
D011506:Proteins