Glycoconjugate and adhesion molecule changes during the cell cycle in a human embryonic epithelial cell line.
The changes in the expression of glycoconjugates and adhesion molecules were studied by selective lectin binding and immunocytochemical reactions in a human embryonic epithelial cell line (EUE cells), synchronized in the cell phases. The results can be summarized as follows: most of the tested lectins display a more diffuse binding for the cytoplasm in G1 than S and G2 phases; in the S and particularly in G2 phases the cytoplasm glycoconjugates are arranged around the nucleus; cells in mitosis always show a strong binding towards all tested lectins. Cellular fibronectin and its receptor beta 1 integrin are well expressed in G1, but the strongest reaction is observed in the S phase. The immunoreactions for laminin and uvomorulin (L-CAM) are poorly positive in all cell cycle phases. The immunocytochemical reaction for heparan sulfate is positive, with a stronger reaction in S and G2 than G1; on the contrary a diffuse staining with the anti-dermatan sulfate proteoglycan antibody appears unchanged during the cell cycle.
Dipartimento di Biologia Animale, Università di Modena, Italy.
A M
AM
Bolognani Fantin
A
A
Franchini
R
R
Malgara
B
B
Rebecchi
A M
AM
Fuhrman Conti
D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002453:Cell Cycle; D002461:Cell Line, Transformed; D004622:Embryo, Mammalian / Q000166:cytology; D004847:Epithelial Cells / Q000166:cytology; D005353:Fibronectins / Q000032:analysis; D006001:Glycoconjugates / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000032:analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted; D037102:Lectins / Q000378:metabolism; D008856:Microscopy, Fluorescence
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D006001:Glycoconjugates; D037102:Lectins; D006735:Horseradish Peroxidase