Intracellular ionic strength regulates the volume sensitivity of a swelling-activated anion channel.
Cell swelling activates an outwardly rectifying anion channel termed VSOAC (volume-sensitive organic osmolyte/anion channel). Regulation of VSOAC by intracellular electrolytes was characterized in Chinese hamster ovary cells by whole cell patch clamp. Elevation of intracellular CsCl concentration from 40 to 180 mM resulted in a concentration-dependent decrease in channel activation. Activation of VSOAC was insensitive to the salt gradient across the plasma membrane, the intracellular concentration of specific anions or cations, and the total intracellular concentration of cations, anions, or electrolytes. Comparison of cells dialyzed with either CsCl or Na2SO4 solutions demonstrated directly that VSOAC activation is modulated by intracellular ionic strength (microi). The relative cell volume at which VSOAC current activation was triggered, termed the channel volume set point, decreased with decreasing ionic strength. At microi = 0.04, VSOAC activation occurred spontaneously in shrunken cells. The rate of VSOAC activation was nearly 50-fold higher in cells with microi = 0.04 vs. those with microi = 0.18. We propose that microi modulates the volume sensor responsible for channel activation.
Laboratory of Cellular and Molecular Physiology, Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee 37232, USA.
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D000818:Animals; D016466:CHO Cells; D002586:Cesium / Q000494:pharmacology; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000494:pharmacology; D006224:Cricetinae; D007424:Intracellular Fluid / Q000502:physiology; D007700:Kinetics; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009994:Osmolar Concentration; D018408:Patch-Clamp Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013431:Sulfates / Q000494:pharmacology
D018118:Chloride Channels; D002712:Chlorides; D013431:Sulfates; C012036:sodium sulfate; D002586:Cesium; D012965:Sodium Chloride; D011189:Potassium Chloride; C028019:cesium chloride