Evaluation of hepatocyte growth factor activator inhibitor expression in normal and malignant colonic mucosa.
Gene expression of hepatocyte growth factor activator inhibitor (HAI), a recently identified Kunitz-type serine proteinase inhibitor, was analyzed in a series of human colorectal carcinoma cell lines and in human colorectal tissues. All of the 14 cell lines derived from adenocarcinoma of the colorectum expressed HAI in vitro, whereas a colon carcinoma cell line of neuroendocrine origin did not. In vivo, HAI was consistently expressed in the normal colorectal mucosa. Although the expression of HAI mRNA was conserved in adenocarcinoma tissues of the colorectum, the levels of expression were decreased in the adenocarcinoma tissues compared to the normal counterparts. There was a tendency towards an inverse correlation, albeit not well defined, between the amounts of HAI mRNA and the tumor progression. Immunohistochemical study indicated that HAI protein is present predominantly on the surface of epithelial cells of the colon and the immunoreactivity was decreased in the adenocarcinoma cells.
Second Department of Pathology, Miyazaki Medical College, Kiyotake, Japan.
H
H
Kataoka
H
H
Uchino
K
K
Denda
N
N
Kitamura
H
H
Itoh
H
H
Tsubouchi
K
K
Nabeshima
M
M
Koono
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003106:Colon / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008875:Middle Aged; D053491:Proteinase Inhibitory Proteins, Secretory; D012333:RNA, Messenger / Q000378:metabolism; D012007:Rectum / Q000378:metabolism*; D014360:Trypsin Inhibitor, Kunitz Soybean; D014407:Tumor Cells, Cultured
D008562:Membrane Glycoproteins; D053491:Proteinase Inhibitory Proteins, Secretory; D012333:RNA, Messenger; C109149:SPINT1 protein, human; C109148:SPINT2 protein, human; D014360:Trypsin Inhibitor, Kunitz Soybean