Characterization of a novel Bax-associated protein expressed in hemopoietic tissues and regulated during thymocyte apoptosis.
Members of the Bcl-2 protein family have been implicated as critical intracellular regulators of apoptosis. Most studies of this protein family have utilized transformed and/or transfected cell lines expressing high levels of these proteins. In the current study, we have analyzed normal murine lymphoid cells and tissues and have detected a previously unreported protein of approximately 16 kDa recognized by an anti-Bax Ab. This 16-kDa protein is abundant in hemopoietic tissues of both wild-type and Bax knock-out mice, it can heterodimerize with Bax in normal lymphocytes, and it is dramatically down-modulated in thymocytes in response to apoptotic stimuli. These results suggest that this protein may have antiapoptotic activity and may participate in the regulation of apoptosis in normal lymphocytes.
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Department of Surgery, University of Pittsburgh School of Medicine, PA 15213, USA.
Hershberger
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PA
McCarthy
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D000818:Animals; D000906:Antibodies / Q000378:metabolism; D017209:Apoptosis / Q000276:immunology*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000276:immunology; D019281:Dimerization; D015536:Down-Regulation / Q000276:immunology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008970:Molecular Weight; D011518:Proto-Oncogene Proteins / Q000276:immunology / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D051028:bcl-2-Associated X Protein
D000906:Antibodies; C494827:Bax protein, mouse; D002352:Carrier Proteins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein