Evaluation of fungal DNA sequence using polymerase chain reaction in infection-free vitreous humor.
We evaluated the vitreous bodies from 14 clinically nonfungal infectious eyes to establish a baseline for the presence of a fungal genome using polymerase chain reaction (PCR). The vitreous samples were all PCR negative, except for one eye with chronic uveitis. Blood samples examined were all PCR negative. We concluded that fungal DNA sequences could not be amplified in vitreous specimens obtained from patients with clinically diagnosed proliferative diabetic retinopathy, macular hole, or epiretinal membrane.
Yanagida Eye Clinic, Shizuoka, Japan.
K
K
Mochizuki
H
H
Matsushita
Y
Y
Hiramatsu
K
K
Yanagida
G
G
Ueda
K
K
Hashimoto
D000328:Adult; D000368:Aged; D002908:Chronic Disease; D017931:DNA Primers / Q000737:chemistry; D004271:DNA, Fungal / Q000032:analysis*; D003937:Diagnosis, Differential; D004587:Electrophoresis, Agar Gel; D015821:Eye Infections, Fungal / Q000382:microbiology*; D005260:Female; D005658:Fungi / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D012164:Retinal Diseases / Q000382:microbiology*; D012189:Retrospective Studies; D014605:Uveitis / Q000382:microbiology; D014821:Vitrectomy; D014822:Vitreous Body / Q000382:microbiology*
D017931:DNA Primers; D004271:DNA, Fungal