Yeast permeabilization as a tool for measurement of in situ enzyme activity: localization of alkaline phosphatase.
The biochemical and ultracytochemical localization of alkaline phosphatases in permeabilized cells of Saccharomyces cerevisiae 257 has been studied. The treatment with non-ionic surfactant Triton X-100 allows the penetration of the substrate into intact yeast cells and thus provides detailed detection of the enzyme activity in ultracytochemical studies.
Bulgarian Academy of Sciences, Institute of Microbiology, Sofia, Bulgaria.
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Spasova
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Galabova
D000469:Alkaline Phosphatase / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D002463:Cell Membrane Permeability; D002848:Chromatography, DEAE-Cellulose; D007425:Intracellular Membranes / Q000201:enzymology; D007700:Kinetics; D017830:Octoxynol; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014617:Vacuoles / Q000201:enzymology
D017830:Octoxynol; D000469:Alkaline Phosphatase