Ascites fluid of nephrotic rats: sodium dodecyl sulphate-polyacrylamide gel electrophoresis protein pattern and the renin-angiotensin system.
1. The concentration of renin and angiotensinogen (Ao) and the activity of angiotensin I-converting enzyme (ACE) was measured in the ascites fluid of nephrotic rats obtained 8 days after puromycin aminonucleoside (PAN) injection. 2. Ascites fluid, serum and urine proteins of these rats were analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). 3. Renin, Ao and ACE were found in the ascites fluid and the percentage of the ratio ascites fluid/(plasma or serum) ranged from 5.9 to 9.9%. The electrophoretic analysis revealed that the ascites fluid contained low (Mr < 66 kDa) and high (Mr < 66 kDa) molecular weight proteins. Albumin and six proteins higher than 66 kDa were present both in the ascites fluid and in serum from nephrotic rats. 4. Data from the study suggest that some proteins in the ascites fluid, including renin, Ao and ACE, come from the plasma. It is possible that the loss of renin, Ao and ACE to the ascites fluid may be playing a role in the metabolic alterations of these three proteins in PAN-nephrotic rats.
Department of Nephrology and Mineral Metabolism, National Institute of Nutrition 'Salvador Zubirán, Mexico City, Mexico.
C
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Cruz
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R
Correa-Rotter
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Mézquita
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ON
Medina-Campos
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J
Pedraza-Chaverrí
D000808:Angiotensinogen / Q000378:metabolism; D000818:Animals; D000903:Antibiotics, Antineoplastic; D001202:Ascitic Fluid / Q000737:chemistry* / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D007202:Indicators and Reagents; D008297:Male; D008970:Molecular Weight; D009404:Nephrotic Syndrome / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011692:Puromycin Aminonucleoside; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D012084:Renin-Angiotensin System / Q000502:physiology*
D000903:Antibiotics, Antineoplastic; D007202:Indicators and Reagents; D011506:Proteins; D000808:Angiotensinogen; D011692:Puromycin Aminonucleoside; D007703:Peptidyl-Dipeptidase A