Non-Hodgkin's lymphomas and myeloid disorders: deletions associated with t(2;5) and t(3;5) detected by FISH.
The nucleophosmin (NPM) gene is involved in two recurrent translocations in hematological malignancies: t(2;5) (p23;q35) in anaplastic large cell lymphoma (ALCL) and t(3;5)(q25.1;q34-35) in myelodysplasia and acute non-lymphocytic leukemia (ANLL). Using eight YACs encompassing the 5q34-q35 region, we could easily detect these two translocations. In both types of translocation, probable unexpected deletions were also discovered downstream of the breakpoint at 5q35.
URA 1967 CNRS, Laboratoire de Cytogénétique et de Génétique Oncologiques, Institut Gustave Roussy, Villejuif, France.
M A
MA
Dragon-Durey
A F
AF
Goguel
C
C
Leonard
R
R
Berger
B
B
Bressac-de-Paillerets
D
D
Fauvet
A
A
Bernheim
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D002893:Chromosomes, Human, Pair 3; D002895:Chromosomes, Human, Pair 5; D005260:Female; D017353:Gene Deletion; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015470:Leukemia, Myeloid, Acute / Q000235:genetics*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008297:Male; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014178:Translocation, Genetic