HIV-1 protease regulation: the role of the major homology region and adjacent C-terminal capsid sequences.
The maturation of human immunodeficiency type-1 virions is accomplished through the proteolytic processing of Gag and GagPol precursor proteins by the viral protease (PR). Since virions must be assembled at the cell surface from uncleaved precursor molecules, intracellular activation of PR must be inhibited. We have previously developed a system where the intracellular activity of PR, associated with GagPol, was inhibited by the expression of Gag in trans. The disproportionate synthesis of Gag inhibits the activation of PR in the cytoplasm. Sequences capable of mediating this inhibition were localized to capsid. In this communication, the region of HIV-1 capsid capable of mediating inhibition was further defined and shown to require the major homology region of capsid within Gag.
Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425-2230, USA.
J
J
Gatlin
S J
SJ
Arrigo
M G
MG
Schmidt
D000595:Amino Acid Sequence; D002213:Capsid / Q000096:biosynthesis* / Q000737:chemistry; D015685:Fusion Proteins, gag-pol / Q000096:biosynthesis; D015683:Gene Products, gag / Q000096:biosynthesis; D016333:HIV Protease / Q000737:chemistry / Q000378:metabolism*; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans
D015685:Fusion Proteins, gag-pol; D015683:Gene Products, gag; D016333:HIV Protease