Histone-like TAFs within the PCAF histone acetylase complex.
PCAF histone acetylase plays a role in regulation of transcription, cell cycle progression, and differentiation. Here, we show that PCAF is found in a complex consisting of more than 20 distinct polypeptides. Strikingly, some polypeptides are identical to TBP-associated factors (TAFs), which are subunits of TFIID. Like TFIID, histone fold-containing factors are present within the PCAF complex. The histone H3- and H2B-like subunits within the PCAF complex are identical to those within TFIID, namely, hTAF(II)31 and hTAF(II)20/15, respectively. The PCAF complex has a novel histone H4-like subunit with similarity to hTAF(II)80 that interacts with the histone H3-like domain of hTAF(II)31. Moreover, the PCAF complex has a novel subunit with WD40 repeats having a similarity to hTAF(II)100.
Ogryzko
V V
VV
Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Kotani
T
T
Zhang
X
X
Schiltz
R L
RL
Howard
T
T
Yang
X J
XJ
Howard
B H
BH
Qin
J
J
Nakatani
Y
Y
D000123:Acetyltransferases / Q000737:chemistry*; D000595:Amino Acid Sequence; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D005656:Fungal Proteins; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006657:Histones / Q000302:isolation & purification*; D006801:Humans; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000302:isolation & purification*; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000302:isolation & purification*
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D046911:Macromolecular Substances; C083323:NGG1 protein, S cerevisiae; D009687:Nuclear Proteins; C075582:SPT3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000123:Acetyltransferases; D051548:Histone Acetyltransferases; D011494:Protein Kinases