Regulation of chondrogenic differentiation of mesenchymes by protein kinase C alpha.
Chondrogenesis of chick limb bud mesenchymes requires the expression and activation of protein kinase C (PKC). This study was performed to identify PKC isoform(s) involved in the regulation chondrogenic differentiation of mesenchymes. Multiple PKC isoforms including alpha, epsilon, zeta and lambda/iota were expressed in mesenchymes derived from chick limb buds. Among the expressed PKC isoforms, the levels of PKC alpha and epsilon were increased during chondrogenic differentiation of mesenchymes. The increase in the expression of these isoforms is more evident in the particulate membrane fraction compared with the cytosolic fraction. Chondrogenesis was blocked by either selective inhibition or down-regulation of PKC alpha. In addition, the degree of chondrogenesis was closely correlated with the expression levels of PKC alpha but not other PKC isoforms expressed in mesenchymes. Thus, the results indicate that only PKC alpha is required for the induction of chondrogenic differentiation
Yang
M S
MS
Department of Biology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Chang
S H
SH
Sonn
J K
JK
Lee
Y S
YS
Kang
S S
SS
Park
T K
TK
Chun
J S
JS
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002642:Chick Embryo; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D018878:Limb Buds; D008648:Mesoderm / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D051571:Protein Kinase C-alpha; D013347:Subcellular Fractions / Q000201:enzymology
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha