Advances in fluorescence in situ hybridization.
The techniques of in situ hybridization (ISH) are widely applied for analyzing the genetic make-up and RNA expression patterns of individual cells. This review focusses on a number of advances made over the last 5 years in the fluorescence ISH (FISH) field, i.e., Fiber-FISH, Multi-colour chromosome painting, Comparative Genomic Hybridization, Tyramide Signal Amplification and FISH with Polypeptide Nucleic Acid and Padlock probes.
Raap
A K
AK
Laboratory for Cytochemistry and Cytometry, Department of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, Netherlands. a.k.raap@mcb.medfac.leidenuniv.nl
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D002869:Chromosome Aberrations; D002874:Chromosome Mapping / Q000379:methods* / Q000639:trends*; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000639:trends*; D008856:Microscopy, Fluorescence; D009693:Nucleic Acid Hybridization / Q000379:methods; D009838:Oligodeoxyribonucleotides; D014439:Tyramine / Q000031:analogs & derivatives
D015342:DNA Probes; D009838:Oligodeoxyribonucleotides; C111551:biotinyltyramide; D001710:Biotin; D014439:Tyramine