Cross-reactivity of an anti-okadaic acid antibody to dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid diol ester.
The cross-reactivity of the 6/50 monoclonal anti-okadaic acid antibody (mAb) to the recently discovered diarrhetic shellfish poisoning (DSP) metabolites dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid (OA) diol ester was determined using a competitive indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of the antibody to these molecules was compared to that with OA; the 6/50 mAb recognized all of these DSP compounds with equal sensitivity within the working range of the antibody (10-100 nM for OA). This confirms the ability of the antibody to detect all DSP compounds when used in analyses including ELISA and immunocytochemistry.
Department of Oceanography, Dalhousie University, Halifax, NS, Canada.
J E
JE
Lawrence
A D
AD
Cembella
N W
NW
Ross
J L
JL
Wright
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003429:Cross Reactions; D004141:Dinoflagellida / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay; D008387:Marine Toxins / Q000737:chemistry / Q000276:immunology*; D019319:Okadaic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D008387:Marine Toxins; C114941:dinophysistoxin 5; C110249:okadaic acid diol ester; C114940:dinophysistoxin 4; D019319:Okadaic Acid