D1/D2 dopamine receptor interaction in membrane abolished by 6-hydroxydopamine lesion.
D1/D2 interaction in rat striatum was investigated by examining the effect of the D2 antagonist spiperone on the binding of [3H]SCH23390 to D1 dopamine (DA) receptors. In the presence of endogenous DA, spiperone blocked D2 receptors, then caused the increase of the binding of [3H]SCH23390 in rat striatal homogenate. After the 6-hydroxydopamine (6-OHDA) lesion, the increase was not found even if in the addition of exogenous DA. The results suggest that the D2 antagonist can modify the interaction between endogenous DA and D1 receptors labeled with [3H]SCH23390, while 6-OHDA lesion may change the state of D1/D2 interaction operating at the receptor level.
Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Science, Shanghai.
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D018663:Adrenergic Agents / Q000494:pharmacology*; D000818:Animals; D001552:Benzazepines / Q000378:metabolism*; D018492:Dopamine Antagonists / Q000378:metabolism*; D008297:Male; D016627:Oxidopamine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000187:drug effects* / Q000378:metabolism; D013134:Spiperone / Q000494:pharmacology; D014793:Visual Cortex / Q000187:drug effects* / Q000378:metabolism
D018663:Adrenergic Agents; D001552:Benzazepines; D018492:Dopamine Antagonists; D017447:Receptors, Dopamine D1; D013134:Spiperone; D016627:Oxidopamine