Case report: DNA fragmentation in glial cells in a cerebral biopsy from a multiple sclerosis patient.
Multiple sclerosis is characterized by myelin destruction and oligodendrocyte loss. The neuropathological hallmark of the disease is the presence of demyelinated plaques in the central nervous system. We have recently found a gliotoxic factor in MS cerebrospinal fluid which induces programmed cell death in vitro, in glial cells. Here we show DNA fragmentation and glial cell death in biopsy samples, obtained from a patient who underwent surgery with suspicion of tumor, and whose disease record, including brain autopsy, demonstrated an active multiple sclerosis. We used the in situ TUNEL technique, a method which sensitively detects the DNA fragmentation accompanying programmed cell death in tissue sections, and compatible with classical fixation techniques. We found intense DNA fragmentation in nuclei of glial cells at-or very near-to the site of demyelination. A double labeling technique showed that glial fibrillary associated protein positive astrocytes may undergo programmed cell death in multiple sclerosis.
INSERM, Laboratoire de Neuromodulations Interactives et Neuropathologies, Paris, France.
N
N
Benjelloun
A
A
Ménard
C
C
Charriaut-Marlangue
K
K
Mokhtari
H
H
Perron
J J
JJ
Hauw
F
F
Rieger
D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001706:Biopsy; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D053938:DNA Fragmentation; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics* / Q000473:pathology; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology*
D005904:Glial Fibrillary Acidic Protein