Use of tick cell culture-derived Anaplasma marginale antigen in a competitive ELISA for serodiagnosis of anaplasmosis.
Anaplasma marginale was propagated in a continuous tick cell line and detergent-solubilized infected cells were used as antigen in a competitive ELISA (C-ELISA) for detection of Anaplasma-specific antibody in bovine sera. Positive control sera competed well (> or = 35% inhibition) with an A. marginale-specific monoclonal antibody for binding to this antigen, while negative sera failed to compete (< 35% inhibition). The C-ELISA was compared to the standard complement-fixation test (CFT) using 2,208 bovine sera. Overall, C-ELISA was more sensitive than CFT (24.9% versus 9.4%), mainly because CFT yielded "suspicious" or "anti-complementary" results in 10.5% of the sera and also failed to identify several vaccinated and carrier cattle that were C-ELISA-positive. The apparent agreement between CFT and C-ELISA was 89.6% and the kappa value was 0.6. These results show that this C-ELISA would be a suitable replacement of the CFT as the standard test for detection of A. marginale antibody.
Oklahoma Animal Disease Diagnostic Laboratory, Oklahoma State University, Stillwater 74078, USA. jsaliki@okstate.edu
J T
JT
Saliki
E F
EF
Blouin
S J
SJ
Rodgers
K M
KM
Kocan
D000709:Anaplasma / Q000276:immunology* / Q000302:isolation & purification; D000712:Anaplasmosis / Q000175:diagnosis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002417:Cattle; D002460:Cell Line; D003168:Complement Fixation Tests; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004912:Erythrocytes / Q000382:microbiology; D018884:Ixodes / Q000382:microbiology; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods
D000911:Antibodies, Monoclonal