Biochemical and molecular characterization of Leishmania parasites isolated from an endemic focus in eastern Sudan.
Twelve Leishmania isolates from visceral leishmaniasis patients in eastern Sudan were characterized using isoenzyme analysis, Southern blotting and polymerase chain reaction (PCR) 'fingerprinting'. Isoenzyme analysis revealed the presence of 3 zymodemes: MON-18, MON-30 and MON-82, corresponding to Leishmania donovani sensu stricto, L. infantum and L. archibaldi (still of uncertain taxonomic status), respectively. Southern blotting and PCR 'fingerprinting' revealed identical patterns for all 3 zymodemes, which were indistinguishable from those of L. donovani s.s.
Department of Biomedical Research, Royal Tropical Institute, Amsterdam, The Netherlands.
L
L
Oskam
F
F
Pratlong
E E
EE
Zijlstra
C C
CC
Kroon
I P
IP
Dedet
P A
PA
Kager
G
G
Schönian
H W
HW
Ghalib
A M
AM
el-Hassan
S E
SE
Meredith
D000818:Animals; D015139:Blotting, Southern; D016054:DNA, Protozoan / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007893:Leishmania donovani / Q000302:isolation & purification*; D018314:Leishmania infantum / Q000302:isolation & purification*; D007898:Leishmaniasis, Visceral / Q000469:parasitology*; D008137:Longitudinal Studies; D016133:Polymerase Chain Reaction; D013397:Sudan
D016054:DNA, Protozoan; D007527:Isoenzymes