Development of transgenic Xenopus laevis with a high C-src gene expression.
Xenopus laevis larvae with an elevated expression of c-src were generated by mating a transgenic X. laevis male frog carrying proviral Rous sarcoma virus (RSV) long terminal repeat (LTR) and most of the pol gene sequences in its sperm DNA and a normal X. laevis female frog. Offspring (15-20%) with a higher dosage of c-Src, detected in disorganized myotomal musculature and in cerebral and spinal neuronal cells by immunohistochemical analysis, developed abnormally, with edemas (in most cases), head deformities, and eye and axial system defects. In the remaining embryos, a small increase in c-src expression seemed to be compatible with normal embryogenesis. The dosage of c-Src correlated with the dosage of RSV LTR integrated in frog DNA as revealed by Southern and polymerase chain reaction (PCR) analyses. Authenticity of the integrated RSV LTR including enhancer sequence was proved by sequencing. Probing of total RNA from aberrant larvae demonstrated several times higher dosage of c-src mRNA in their tissues than in control tadpoles. We hypothesize that the integrated RSV regulatory sequences can stimulate the expression of c-src proto-oncogene of X. laevis above a threshold that interferes with the early developmental program of frog embryos.
Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague.
M
M
Takác
V
V
Habrová
J
J
Mácha
N
N
Césková
J
J
Jonák
D000818:Animals; D030801:Animals, Genetically Modified; D001358:Avian Sarcoma Viruses / Q000235:genetics; D004279:DNA, Viral; D005260:Female; D015870:Gene Expression; D016679:Genome, Viral; D008297:Male; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000096:biosynthesis* / Q000235:genetics; D011533:Proviruses / Q000235:genetics; D012333:RNA, Messenger; D014982:Xenopus laevis / Q000254:growth & development
D004279:DNA, Viral; D012333:RNA, Messenger; D016392:Proto-Oncogene Proteins pp60(c-src)