A new breast cancer cell line of epithelial origin is tumorigenic in athymic mice.
We have established a newly derived breast cell line, called G8. This line was obtained by means of the neoplastic transformation of murine breast cells through exposure to the carcinogen NMU and its subsequent inoculation in athymic nude mice. This cell line is highly tumorigenic in nude mice. The tumors that developed in the mice were mammary adenocarcinomas. The morphological and ultrastructural pattern suggested that these cells are of epithelial origin. Immunocytochemical studies revealed that the cells express desmoplakin, vimentin, epithelial membrane antigen, fibronectin and actin. The possibility of maintaining this cell line in vivo by means of xenografts provides very valuable material for breast cancer research, as not only does tumor growth in vivo provide the ideal material for the testing of new therapies, but it also enables the study of various important interactions between the tumor and the host tissues including tumor-stroma interactions such as angiogenesis.
Department of Morphology, Medicine School, University of Zaragoza, Spain. bconde@posta.unizar.es
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Gascon
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Alcala
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Lorenzo
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D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D004848:Epithelium / Q000473:pathology; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology* / Q000648:ultrastructure; D008770:Methylnitrosourea; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured
D008770:Methylnitrosourea