Macaque MDC family of proteins: sequence analysis, tissue distribution and processing in the male reproductive tract.
A large number of sequence-related, cysteine-rich membrane proteins containing metalloproteinase-like and disintegrin-like domains (the MDC protein family) have been identified in mammalian tissues from a variety of species. Previous studies in the macaque (Macaca fascicularis) have led to the cDNA cloning and sequence analysis of a number of MDC proteins which are abundantly expressed in the male reproductive tract. We now describe the distribution of seven of these macaque MDC transcripts in a range of different tissues. This description includes a novel macaque testis-derived MDC, tMDC III, whose full-length sequence is reported for the first time. In addition, polyclonal antisera have been used to localize a number of these MDC proteins to spermatogenic cells in testis sections, and to demonstrate their processing on the sperm surface during epididymal transit.
Department of Biochemistry, University of Bristol, School of Medical Sciences, UK.
J
J
Frayne
J A
JA
Jury
H L
HL
Barker
A C
AC
Perry
R
R
Jones
L
L
Hall
D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D010450:Endopeptidases / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism; D000072196:Fertilins; D019084:Fluorescent Antibody Technique, Indirect; D005837:Genitalia, Male / Q000254:growth & development / Q000378:metabolism*; D006168:Guinea Pigs; D007106:Immune Sera; D008252:Macaca fascicularis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012741:Sexual Maturation; D013045:Species Specificity; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
D007106:Immune Sera; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; C088719:tMDC I protein, Macaca fascicularis; C096913:tMDC IV protein, Macaca fascicularis; D010450:Endopeptidases; D051722:ADAM Proteins; C108914:Adam18 protein, rat; C484218:Adam5 protein, rat; C484106:Adam7 protein, mouse; C076862:Adam7 protein, rat; D000072196:Fertilins; D008666:Metalloendopeptidases