Macaque MDC family of proteins: sequence analysis, tissue distribution and processing in the male reproductive tract.
A large number of sequence-related, cysteine-rich membrane proteins containing metalloproteinase-like and disintegrin-like domains (the MDC protein family) have been identified in mammalian tissues from a variety of species. Previous studies in the macaque (Macaca fascicularis) have led to the cDNA cloning and sequence analysis of a number of MDC proteins which are abundantly expressed in the male reproductive tract. We now describe the distribution of seven of these macaque MDC transcripts in a range of different tissues. This description includes a novel macaque testis-derived MDC, tMDC III, whose full-length sequence is reported for the first time. In addition, polyclonal antisera have been used to localize a number of these MDC proteins to spermatogenic cells in testis sections, and to demonstrate their processing on the sperm surface during epididymal transit.
Frayne
J
J
Department of Biochemistry, University of Bristol, School of Medical Sciences, UK.
Jury
J A
JA
Barker
H L
HL
Perry
A C
AC
Jones
R
R
Hall
L
L
D051722:ADAM Proteins; D000595:Amino Acid Sequence; D000818:Animals; D019281:Dimerization; D010450:Endopeptidases / Q000378:metabolism; D004822:Epididymis / Q000378:metabolism; D000072196:Fertilins; D019084:Fluorescent Antibody Technique, Indirect; D005837:Genitalia, Male / Q000254:growth & development / Q000378:metabolism*; D006168:Guinea Pigs; D007106:Immune Sera; D008252:Macaca fascicularis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008666:Metalloendopeptidases / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009928:Organ Specificity; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012741:Sexual Maturation; D013045:Species Specificity; D013091:Spermatogenesis; D013094:Spermatozoa / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
D007106:Immune Sera; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D011506:Proteins; D012333:RNA, Messenger; C088719:tMDC I protein, Macaca fascicularis; C096913:tMDC IV protein, Macaca fascicularis; D010450:Endopeptidases; D051722:ADAM Proteins; C108914:Adam18 protein, rat; C484218:Adam5 protein, rat; C484106:Adam7 protein, mouse; C076862:Adam7 protein, rat; D000072196:Fertilins; D008666:Metalloendopeptidases