Bioelectrochemical monitoring of phenols and aromatic amines in flow injection using novel plant peroxidases.
An amperometric flow system combined with a glucose oxidase-mutarotase reactor was optimized and used to determine aromatic amines and phenols using peroxidase-modified graphite electrodes. An increase in currents upon injection of the analyzed substrate was shown to be approximated by a Michaelis-Menten type dependence. The detection limit was calculated as 3 times the noise, and the sensitivity was calculated as Imax/K(m)app. Commercially available horseradish peroxidase was compared with tobacco anionic and peanut cationic peroxidases for determination of aromatic amines and phenols. Detection limits of 10 nM for determination of o-aminophenol and o- and p-phenylenediamine achieved with a tobacco peroxidase-modified electrode give a promise for further improvements in sensitivities and detection limits of biosensors.
Department of Analytical Chemistry, Faculty of Chemistry, University of Bucharest, Sos. Panduri, Romania.
F D
FD
Munteanu
A
A
Lindgren
J
J
Emnéus
L
L
Gorton
T
T
Ruzgas
E
E
Csŏregi
A
A
Ciucu
R B
RB
van Huystee
I G
IG
Gazaryan
L M
LM
Lagrimini
D000814:Aniline Compounds / Q000032:analysis*; D000818:Animals; D010367:Arachis / Q000201:enzymology; D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques; D002238:Carbohydrate Epimerases; D017022:Flow Injection Analysis; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D007668:Kidney / Q000201:enzymology; D010636:Phenols / Q000032:analysis*; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D013237:Stereoisomerism; D013552:Swine; D014026:Nicotiana / Q000201:enzymology
D000814:Aniline Compounds; D010636:Phenols; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase