Molecular genotyping by pulsed-field gel electrophoresis of restricted genomic DNA of strains of Taylorella equigenitalis isolated in Ireland and in the United States.
The profiles, after digestion with ApaI or NotI and pulsed-field gel electrophoresis (PFGE), of genomic DNA from 18 strains of Taylorella equigenitalis isolated in Ireland were of three different types. The 13 strains in one of these types gave PFGE profiles identical to that of an American prototype strain, Kentucky 188, but different from strain NCTC11184T and from a strain isolated in Japan. Eight additional strains isolated in the United States gave four distinct types of genomic PFGE profiles. All four types were different from that of T. equigenitalis NCTC11184T or that of the Japanese strain. The profile of three strains of one type was identical to that of Kentucky 188.
Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, Sagamihara, Japan.
M
M
Matsuda
T
T
Miyazawa
J E
JE
Moore
T C
TC
Buckley
L A
LA
Thomas
D000818:Animals; D004269:DNA, Bacterial / Q000032:analysis* / Q000737:chemistry; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field / Q000662:veterinary; D004716:Endometritis / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D005838:Genotype; D006190:Haemophilus / Q000145:classification* / Q000235:genetics; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D007494:Ireland; D015231:Sexually Transmitted Diseases, Bacterial / Q000382:microbiology / Q000662:veterinary*; D014481:United States
D004269:DNA, Bacterial; D015252:Deoxyribonucleases, Type II Site-Specific; C038565:GCGGCCGC-specific type II deoxyribonucleases; C038485:GGGCCC-specific type II deoxyribonucleases