UGA4 gene expression in Saccharomyces cerevisiae depends on cell growth conditions.
In the yeast Saccharomyces cerevisiae, gamma-aminobutyric acid (GABA) transport is mediated by three permeases: the general amino acid permease GAP1, the proline permease PUT4 and the specific GABA permease UGA4. Expression of UGA4 gene is induced in the presence of GABA. We started a comparative study about UGA4 gene induction in cells grown on different culture media. Results presented here indicate that under certain growth conditions UGA4 permease is constitutive. Therefore, we demonstrate that in S. cerevisiae the UGA4 gene expression depends on cell growth conditions and that its synthesis not always depends on the presence of GABA.
Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONCIET, FCEyN, UBA), Ciudad Universitaria, Pabellón II, Buenos Aires, Argentina.
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Bermúdez Moretti
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Correa García
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Batlle
D026921:Amino Acid Transport Systems, Neutral; D002455:Cell Division; D003470:Culture Media; D050485:GABA Plasma Membrane Transport Proteins; D015966:Gene Expression Regulation, Fungal; D006863:Hydrogen-Ion Concentration; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D027361:Organic Anion Transporters; D011392:Proline / Q000378:metabolism / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins; D015533:Transcriptional Activation; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D026921:Amino Acid Transport Systems, Neutral; D003470:Culture Media; D050485:GABA Plasma Membrane Transport Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D029701:Saccharomyces cerevisiae Proteins; C513599:UGA4 protein, S cerevisiae; D005680:gamma-Aminobutyric Acid; C033082:proline transporter; D011392:Proline