[Isolation of human mononuclear cells from bone marrow, peripheral blood and cord blood using Ficoll-Paque (Pharmacia) and Gradisol L (Polfa). Comparative study].
The aim of this study was to compare the effectiveness of Ficoll-Paque (Pharmacia), and Gradisol L (Polfa) solutions in isolating human mononuclear cells from: bone marrow, peripheral, and cord blood samples. We found employing both FACS analysis, and in vitro clonogenic tests for isolated cells, that both solutions allow to isolate identical cell populations. Therefore Gradisol-L (Polfa). which is much cheaper than Ficoll-Paque should be widely recommended in strategies for isolating hematopoietic mononuclear cells for both clinical and experimental purposes.
Zakładu Patologii Komórki Pomorskiej Akademii Medycznej.
B
B
Machaliński
M
M
Honczarenko
A
A
Gontarewicz
M Z
MZ
Ratajczak
D001854:Bone Marrow Cells / Q000166:cytology*; D002999:Clone Cells; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D009000:Monocytes / Q000166:cytology*; D011044:Poland