Analysis of the 5' flanking sequence of the human norepinephrine transporter gene.
The human norepinephrine transporter (NET) gene was cloned and structurally analyzed. The far 5' fragment containing exon 1 (a non-coding exon) and exon 2 was sequenced. The transcription start site of the gene in human brain stem tissue was determined by primer extension analysis. It was found that the gene could be transcribed from multiple starting points. The 5' flanking sequence contains a proximal G-C rich region, one possible GSG element and several SP1 sites. However it does not contain TATA box and CAAT box motifs. Gel shift analysis with nuclear extracts from different tissues of mouse shows that the G-C rich region may be involved in tissue specific expression of the gene.
Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.
F
F
Huang
J
J
Fei
S K
SK
Ma
L H
LH
Zhu
Z P
ZP
Liu
G Q
GQ
Cai
Z C
ZC
Ye
L H
LH
Guo
D000818:Animals; D001933:Brain Stem; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D005091:Exons; D005786:Gene Expression Regulation; D006801:Humans; D007668:Kidney; D008099:Liver; D051379:Mice; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000378:metabolism; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016329:Sp1 Transcription Factor / Q000235:genetics; D027981:Symporters; D016385:TATA Box / Q000235:genetics; D016335:Zinc Fingers / Q000235:genetics
D002352:Carrier Proteins; D017362:Cyclic AMP Response Element-Binding Protein; D050484:Norepinephrine Plasma Membrane Transport Proteins; D009687:Nuclear Proteins; C493227:SLC6A2 protein, human; C493228:Slc6a2 protein, mouse; D016329:Sp1 Transcription Factor; D027981:Symporters; D009638:Norepinephrine