Nucleolar localization of BRCA1 protein in human breast cancer.
Both a rabbit polyclonal BRCA1 antibody, K-18, and a mouse monoclonal BRCA1 antibody, AP 16, produced nucleolar epithelial cell staining on frozen tissue sections of human infiltrating mammary carcinomas. There was much less BRCA1 antibody staining in normal tissues; however, 2 intraductal tumors and a papilloma, found in proximity to the carcinomas showed considerable nucleolar immunoreactivity. MCF-7 cells fixed in methanol and immunostained with the same two antibodies also revealed nucleolar staining, however, after 4% paraformaldehyde fixation for three minutes, there were many fewer nuclei stained. Antigen retrieval methods on formalin-fixed, paraffin-embedded specimens produced tumor cell cytoplasmic staining with AP 16 and nuclear staining in both tumor and normal epithelial cells with another BRCA1 monoclonal antibody, SG 11.
Tulchin
N
N
Department of Pathology, Mount Sinai School of Medicine, New York, NY 10029, USA.
Ornstein
L
L
Bleiweiss
I J
IJ
Mendlowitz
M
M
Weinstein
E
E
Dikman
S H
SH
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal; D019313:BRCA1 Protein / Q000378:metabolism*; D001940:Breast / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism* / Q000648:ultrastructure; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000378:metabolism*; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008875:Middle Aged; D011817:Rabbits; D012016:Reference Values
D000911:Antibodies, Monoclonal; D019313:BRCA1 Protein