Comparison of membrane filtration and multiple-tube fermentation by the colilert and enterolert methods for detection of waterborne coliform bacteria, Escherichia coli, and enterococci used in drinking and bathing water quality monitoring in southern sweden.
A total of 338 water samples, 261 drinking water samples and 77 bathing water samples, obtained for routine testing were analyzed in duplicate by Swedish standard methods using multiple-tube fermentation or membrane filtration and by the Colilert and/or Enterolert methods. Water samples came from a wide variety of sources in southern Sweden (Skåne). The Colilert method was found to be more sensitive than Swedish standard methods for detecting coliform bacteria and of equal sensitivity for detecting Escherichia coli when all drinking water samples were grouped together. Based on these results, Swedac, the Swedish laboratory accreditation body, approved for the first time in Sweden use of the Colilert method at this laboratory for the analysis of all water sources not falling under public water regulations (A-krav). The coliform detection study of bathing water yielded anomalous results due to confirmation difficulties. E. coli detection in bathing water was similar by both the Colilert and Swedish standard methods as was fecal streptococcus and enterococcus detection by both the Enterolert and Swedish standard methods.
KM Lab AB, 251 07 Helsingborg, Sweden. karl.eckner@colifast.no
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KF
Eckner
D001431:Bacteriological Techniques; D003470:Culture Media; D004755:Enterobacteriaceae / Q000254:growth & development / Q000302:isolation & purification*; D016983:Enterococcus / Q000254:growth & development / Q000302:isolation & purification; D004926:Escherichia coli / Q000254:growth & development / Q000302:isolation & purification*; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005374:Filtration; D007785:Lactose / Q000378:metabolism; D008851:Micropore Filters; D013289:Streptococcaceae / Q000254:growth & development / Q000302:isolation & purification*; D013291:Streptococcus / Q000254:growth & development / Q000302:isolation & purification; D013548:Sweden; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*
D003470:Culture Media; D007785:Lactose