Preparation of hydrogel microspheres by coacervation of aqueous polyphosphazene solutions.
A new method of preparing ionically cross-linked polyphosphazene hydrogel microspheres which enables effective control over microsphere size distribution has been developed. The synthesized microspheres can be used in protein delivery and, especially, as vaccine delivery vehicles. A new technique utilizes the ability of aqueous solutions of poly[di(carboxylatophenoxy)phosphazene] (PCPP) to form coacervate microdroplets upon addition of sodium chloride. These microdroplets are then stabilized via polymer cross-linking with calcium ions. It was demonstrated that the method enables efficient microencapsulation of proteins. It was also shown that microsphere size can be controlled through the manipulation of microencapsulation conditions. The process is simple, highly reproducible and generates microspheres with a narrower microsphere size distribution, compared to the previous technologies.
Andrianov
A K
AK
Virus Research Institute Inc., Cambridge, MA 02138, USA.
Chen
J
J
Payne
L G
LG
D001672:Biocompatible Materials / Q000737:chemistry*; D002122:Calcium Chloride / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D008863:Microspheres; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry
D001672:Biocompatible Materials; C070410:Ca-poly(bis(carboxylatophenoxy)phosphazene); D009943:Organophosphorus Compounds; D011108:Polymers; C108974:poly(phosphazene); D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D002122:Calcium Chloride