Genotoxicity of an extract of Calendula officinalis L.
A fluid extract of Calendula officinalis L. displayed genotoxic properties when assayed for mitotic segregation in the heterozygous diploid D-30 of Aspergillus nidulans. The extract of Calendula exhibited dose-dependent toxicity and genotoxicity (both mitotic crossing-over and chromosome malsegregation being observed) to Aspergillus in the range of five plate concentrations from 0.1 to 1.0 mg of solids/ml assayed. Mutagenicity testing with the Salmonella/microsome assay in strains TA 1535, TA 1537, TA 98 and TA 100 was negative in a plate incorporation protocol, with concentrations ranging from 50 to 5000 microg/plate (+/- S9). The mouse bone marrow micronucleus test, where the extract was dosed orally up to 1 g/kg for 2 days, was also negative.
Centro de Investigación y Desarrolo de Medicamentos, Ciudad de La Habana, Cuba.
A
A
Ramos
A
A
Edreira
A
A
Vizoso
J
J
Betancourt
M
M
López
M
M
Décalo
D000818:Animals; D001230:Aspergillus / Q000187:drug effects / Q000235:genetics; D001853:Bone Marrow / Q000187:drug effects / Q000648:ultrastructure; D003462:Cuba; D004305:Dose-Response Relationship, Drug; D005260:Female; D008297:Male; D051379:Mice; D015162:Micronucleus Tests; D008938:Mitosis / Q000187:drug effects / Q000235:genetics; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal / Q000633:toxicity*; D012475:Salmonella / Q000187:drug effects / Q000235:genetics
D010936:Plant Extracts