Evolution of the apolipoprotein E receptor 2 gene by exon loss.
The apolipoprotein E receptor 2 (apoER2) gene consists of a mosaic of exons, which may have been assembled by "exon shuffling." Analysis of apoER2 transcripts in several species reveals a lost repeat in the ligand-binding domain of primate apoER2. A pseudo-exon found in the primate apoER2 genes corresponds to the lost repeat but contains a crucial deletion that leads to a translational frameshift. The pseudo-exon sequence in primary transcripts of the human apoER2 gene is shown to be abolished by exon skipping due to two nucleotide substitutions at the 5'-splice donor adjacent to the pseudo-exon. These data suggest the occurrence of exon loss in the evolution of the primate apoER2 gene.
Kim
H J
HJ
Tohoku University Gene Research Center, Aoba, Sendai, 981-8555, Japan.
Kim
D H
DH
Magoori
K
K
Saeki
S
S
Yamamoto
T T
TT
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002144:Callithrix; D004247:DNA; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics*; D017353:Gene Deletion; D014644:Genetic Variation; D006801:Humans; D026502:LDL-Receptor Related Proteins; D008024:Ligands; D051379:Mice; D008969:Molecular Sequence Data; D018110:Receptors, Lipoprotein / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D026502:LDL-Receptor Related Proteins; D008024:Ligands; D018110:Receptors, Lipoprotein; C098773:low density lipoprotein receptor-related protein 8; D004247:DNA