On the origin of membrane vesicles in gram-negative bacteria.
It is proposed that the genesis of extracellular membrane vesicles in Gram-negative bacteria is a result of cell wall turnover. Peptidoglycan turnover would cause a turgor on the outer membrane, causing the outer membrane to bulge and finally bleb. Mechanical motion would then shear the blebs into the culture medium.
Department of Microbiology and Immunology, University of Louisville, KY 40292, USA.
L
L
Zhou
R
R
Srisatjaluk
D E
DE
Justus
R J
RJ
Doyle
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D006801:Humans; D009112:Muramic Acids / Q000032:analysis; D010455:Peptides / Q000032:analysis; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis
D001425:Bacterial Outer Membrane Proteins; D009112:Muramic Acids; D010455:Peptides; D011506:Proteins