Electrochemical detection of microcystins, cyanobacterial peptide hepatotoxins, following high-performance liquid chromatography.
A novel amperometric HPLC detection method for the cyanobacterial (blue-green algal) peptide toxins microcystin-LR, -YR and -RR was developed. Purified microcystins and cyanobacterial extracts were chromatographed using an internal surface reversed-phase column with acetate- and phosphate-based mobile phase systems. Electrochemical oxidation reactions at 1.20 V vs. Ag/AgCl (glassy carbon working electrode) were show to originate in arginine and tyrosine residues of microcystins.
Meriluoto
J
J
Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
Kincaid
B
B
Smyth
M R
MR
Wasberg
W
W
D001427:Bacterial Toxins / Q000032:analysis*; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D000458:Cyanobacteria / Q000737:chemistry*; D004563:Electrochemistry; D007202:Indicators and Reagents; D052998:Microcystins; D010456:Peptides, Cyclic / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D001427:Bacterial Toxins; D007202:Indicators and Reagents; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin