Heteronuclear correlation experiments for the determination of one-bond coupling constants.
Spin-state selective experiments, HSQC-alpha/beta and CT-HMQC-alpha/beta, are proposed for the simple and rapid measurement of scalar one-bond coupling constants in two-dimensional, 1H-detected 15N-1H or 13C-1H correlation experiments based on HSQC and HMQC schemes. Pairs of subspectra are obtained, containing either the high-field or the low-field component of the doublet representing the one-bond coupling constant. The subspectral editing procedure retains the full sensitivity of HSQC and HMQC spectra recorded without heteronuclear decoupling during data acquisition, with a spectral resolution similar to that of decoupled spectra.
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
P
P
Andersson
K
K
Nordstrand
M
M
Sunnerhagen
E
E
Liepinsh
I
I
Turovskis
G
G
Otting
D001426:Bacterial Proteins / Q000737:chemistry; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000295:instrumentation / Q000379:methods*; D017433:Protein Structure, Secondary; D012097:Repressor Proteins / Q000737:chemistry
C087018:ArgR protein, Bacteria; C490177:ArgR protein, E coli; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012097:Repressor Proteins