Expression of the SU glycoprotein of maedi visna virus in baculovirus.
The envelope glycoprotein, gp 135, of the ovine lentivirus maedi visna virus is the main target for a specific antibody response in vivo, however, little is known about the specific regions of gp 135 which elicit this response. Research on the function of gp 135 has been hampered by the lack of reagents to study such structure/function relationships. We have used a baculovirus expression system to express gp 135 lacking the viral signal sequence. This recombinant protein is glycosylated and recognised by immune sera from clinically affected animals.
Department of Veterinary Pathology, University of Edinburgh, U.K.
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Carter
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Dalziel
D000818:Animals; D016367:Baculoviridae / Q000235:genetics / Q000276:immunology; D006031:Glycosylation; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology; D012756:Sheep; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D014790:Visna-maedi virus / Q000235:genetics* / Q000254:growth & development / Q000276:immunology / Q000378:metabolism
D014759:Viral Envelope Proteins; C089034:glycoprotein 135, Maedi visna virus