Isolation and genetic characterization of the porcine apolipoprotein E gene.
The present report describes the isolation and genetic characterization of the porcine apolipoprotein E (apo-E) gene. A single positive recombinant phage clone containing a 10.7-kb insert was isolated from a porcine genomic library, and a 4.2-kb fragment was subcloned and sequenced. The 4.2-kb fragment contained the entire apo-E gene in addition to upstream and downstream sequences (GenBank accession no. 470240). The porcine apo-E gene is made up of four exons and three introns, and encodes a preapo-E protein comprised of a signal peptide of 18 amino acids and a mature protein of 299 amino acids. The porcine apo-E gene contains a (CG)13 microsatellite marker within intron three. This microsatellite is moderately polymorphic, and at least four alleles were evident at this locus among 10 animals from each of the Yorkshire, Hampshire, Landrace and Duroc breeds. Finally, localization of the porcine apo-E gene to chromosome 6 band q2.1 was determined by fluorescent in situ hybridization and confirmed by genetic linkage analysis.
Ramsoondar
J J
JJ
Department of Veterinary Anatomy and Public Health, College of Veterinary Medicine and Center for Animal Genetics, Texas A & M University, College Station 77843-4458, USA.
Rucker
E B
EB
Vasquez
J C
JC
Gallagher
D S
DS
Grimm
D R
DR
Lunney
J K
JK
Schook
L B
LB
Piedrahita
J A
JA
D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002874:Chromosome Mapping / Q000662:veterinary*; D003001:Cloning, Molecular; D017931:DNA Primers; D004195:Disease Models, Animal; D005091:Exons / Q000235:genetics; D005787:Gene Frequency; D017404:In Situ Hybridization, Fluorescence / Q000662:veterinary; D007438:Introns / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D008969:Molecular Sequence Data; D021382:Protein Sorting Signals / Q000235:genetics; D015183:Restriction Mapping / Q000662:veterinary*; D017422:Sequence Analysis, DNA / Q000662:veterinary; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000235:genetics*
D001057:Apolipoproteins E; D017931:DNA Primers; D021382:Protein Sorting Signals