Uptake of N,N-dimethyl-2-phenylethylene HCl into acini cells removed from rabbit lacrimal glands.
Acini cells were obtained from the lacrimal gland of the white New Zealand rabbit. Following isolation and purification, the cells were used to study the uptake of N,N'-dimethyl-2-phenylethylamine HCl (AF2975), which was found to be sodium- and proton-independent, but energy-dependent. Uptake was mainly accomplished via a carrier-mediated transport system for which a Km of 8.72+/-0.96 mM, a Vmax of 602.6+/-41.3 nmol/mg of protein/min, and an exponential coefficient of 2.55+/-0.46 were obtained following a least squares nonlinear fit to the Hill equation. With the addition of the metabolic inhibitors, sodium azide or 2,4-dinitrophenol, the initial uptake rates were reduced from the control experiments by 35.7% and 26.2%, respectively.
Division of Pharmaceutics, College of Pharmacy, The University of Iowa, Iowa City 52242-1112, USA.
R D
RD
Schoenwald
Y S
YS
Yang
E
E
Xia
C F
CF
Barfknecht
D019297:2,4-Dinitrophenol; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D007765:Lacrimal Apparatus / Q000166:cytology / Q000378:metabolism*; D016018:Least-Squares Analysis; D016014:Linear Models; D010627:Phenethylamines / Q000493:pharmacokinetics*; D011817:Rabbits; D019810:Sodium Azide / Q000493:pharmacokinetics; D013053:Spectrophotometry; D014475:Uncoupling Agents / Q000493:pharmacokinetics
D010627:Phenethylamines; D014475:Uncoupling Agents; C043554:N,N-dimethylphenylethylamine; D019810:Sodium Azide; D019297:2,4-Dinitrophenol