Clinical application of K-ras oncogene mutations in pancreatic carcinoma: detection of micrometastases.
Pancreatic adenocarcinomas are known to have a high incidence of K-ras gene mutation. We summarize our efforts to detect micrometastases through a search for mutated K-ras oncogene in liver tissues, peritoneal washings, para-aortic lymph nodes, and perioperative peripheral blood. Two-stage polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis were used to detect K-ras oncogene mutations at codon 12. Our results suggest that PCR/RFLP analysis is potentially highly sensitive for the detection of micrometastases in various tissues and might be of value in the diagnosis, treatment and follow-up of metastases in other organs with pancreatic adenocarcinoma.
Department of Surgery II, Nagoya University School of Medicine, Japan.
S
S
Nomoto
A
A
Nakao
N
N
Ando
S
S
Takeda
Y
Y
Kasai
S
S
Inoue
T
T
Kaneko
H
H
Takagi
D000230:Adenocarcinoma / Q000235:genetics / Q000556:secondary*; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D004252:DNA Mutational Analysis; D005260:Female; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology*; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016283:Proto-Oncogene Proteins p21(ras) / Q000032:analysis*
D014408:Biomarkers, Tumor; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)