Structural analysis of the amplified IFN-beta and DHFR genes in a Chinese hamster ovary cell line using multicolour FISH analysis.
Multicolour FISH was used to get insight into the structural arrangement of a homogeneously staining region which bears the co-transfected and subsequently co-amplified IFN-beta and DHFR genes in a CHO cell line. On metaphase chromosomes an arrangement of multiple bands with regular spacing is revealed. On extended chromatin fibres a cluster of directly repeated and interspersed IFN-beta and DHFR genes is visible. Up to three clusters were found arranged in tandem. The different chromosomal mechanisms leading to gene amplification are discussed.
Dixkens
C
C
Abteilung Medizinische Genetik, Universität Ulm.
Posseckert
G
G
Keller
T
T
Hameister
H
H
D000818:Animals; D015139:Blotting, Southern; D016466:CHO Cells; D002874:Chromosome Mapping; D006224:Cricetinae; D015342:DNA Probes; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D005784:Gene Amplification; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D016899:Interferon-beta / Q000235:genetics*; D007621:Karyotyping; D008727:Methotrexate; D011994:Recombinant Proteins / Q000235:genetics; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*; D014966:Xanthenes
D015342:DNA Probes; D005456:Fluorescent Dyes; D011994:Recombinant Proteins; D014966:Xanthenes; D016899:Interferon-beta; C034657:Texas red; D013762:Tetrahydrofolate Dehydrogenase; D016650:Fluorescein-5-isothiocyanate; D008727:Methotrexate