Is CD30 (Ki-1) immunostaining in cutaneous eruptions useful as a marker of Th1 to Th2 cytokine switching and/or as a marker of advanced HIV-1 disease?
CD30 is a member of the tumour necrosis factor/nerve growth factor receptor superfamily, which is expressed on CD4+ and CD8+ T-cell clones which produce T helper (Th) 2-type cytokines. It has been proposed that disease progression in HIV-1 is associated with Th1 to Th2 cytokine switching. In 70 cutaneous biopsies from HIV-1 positive patients in different stages of disease, we performed a battery of immunohistochemical stains. These included antibodies to CD3, UCHL-1, OPD-4, L-26, KP-1 and CD30 (Ki-1). In addition, we used a similar battery of stains on cutaneous biopsies of HIV-1 negative patients with inflammatory dermatoses which are established as Th1 or Th2 dominant, e.g. polar leprosy. CD30+ cells were rarely present in early stages of HIV-1 disease, but commonly present in later stages of disease. However, there were cases of late HIV-1 disease which did not contain CD30+ cells. Increased numbers of CD30+ cells were more commonly seen in later stages of HIV-1 disease. However, the expression of CD30 appeared to be better in predicting other established Th2 cutaneous infiltrates in HIV-1 negative patients than in predicting a Th2 cutaneous cytokine pattern in advanced HIV-1 disease.
Smith
K J
KJ
National Naval Medical Center, Bethesda, MD 20889-5600, USA.
Barrett
T L
TL
Neafie
R
R
Tomaszewski
M M
MM
Yeager
J
J
Nelson
A
A
Wagner
K F
KF
Skelton
H G
HG
D015415:Biomarkers; D018791:CD4 Lymphocyte Count; D016207:Cytokines / Q000276:immunology*; D003872:Dermatitis / Q000276:immunology* / Q000821:virology; D018450:Disease Progression; D015658:HIV Infections / Q000150:complications / Q000276:immunology*; D015497:HIV-1; D006801:Humans; D017730:Ki-1 Antigen / Q000378:metabolism*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology; D007918:Leprosy / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D015415:Biomarkers; D016207:Cytokines; D017730:Ki-1 Antigen