Synthesis of testosterone and 5alpha-androstanediols during nutritionally stimulated gonadal growth in Lytechinus variegatus lamarck (Echinodermata:Echinoidea).
Although sex steroids and steroid converting enzymes have been found in echinoids, the relationship between steroids and reproduction has not been demonstrated. On days 0, 4, 8, 16, 32, and 48 of feeding, the gonads of previously starved Lytechinus variegatus were excised and incubated with [3H]androstenedione for 0.5 h to determine if changes in steroidogenic capacity are correlated with gonadal growth. Total rates of androstenedione conversion in the testes and ovaries increased significantly during feeding. In addition, the types and relative quantities of metabolites synthesized varied, suggesting that androstenedione metabolism is influenced by nutritional status. Both testes and ovaries synthesized testosterone, 5alpha-androstane-3alpha,17beta-diol, and 5alpha-androstane-3beta, 17beta-diol (5alpha-adiols), 5alpha-androstanedione, epiandrosterone, and androsterone on all days of feeding. In the testes, the relative quantities of testosterone and 5alpha-adiols increased greatly on day 4 of feeding. In contrast, in the ovaries testosterone synthesis was not detectable on day 4, although the relative quantities of 5alpha-adiols increased threefold. The sex-specific changes in the synthesis of these metabolites reflect a shift in the metabolic pathway indicated by changes in the relative enzyme activity indices for 5alpha-reductase (5alpha-R) (necessary for the synthesis of 5alpha-reduced androgens) and 3alpha/beta-hydroxysteroid dehydrogenase (3alpha/beta-HSDs, necessary for the synthesis of 3alpha- or 3beta-hydroxylated androgens). In both testes and ovaries the relative activities of 5alpha-R and 3alpha/beta-HSD increased on day 4 of feeding. The physiological significance of changes in androstenedione metabolism may be associated with the initiation of biosynthetic processes associated with gametogenesis.
University of Alabama at Birmingham, Birmingham, Alabama, 35294-1170, USA.
K M
KM
Wasson
G A
GA
Hines
S A
SA
Watts
D000731:Androstanes / Q000378:metabolism; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D042944:Cholestenone 5 alpha-Reductase; D002855:Chromatography, Thin Layer; D004447:Echinodermata / Q000378:metabolism*; D005043:Etiocholanolone / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005500:Follow-Up Studies; D008297:Male; D010053:Ovary / Q000254:growth & development* / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D013737:Testis / Q000254:growth & development* / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis*
D000731:Androstanes; D013739:Testosterone; C006576:androstane-3,17-dione; D005043:Etiocholanolone; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase