Cryo-microtome sections of coproculture larvae of Strongyloides stercoralis and Strongyloides ratti as antigen sources for the immunodiagnosis of human strongyloidiasis.
Cryo-microtome sections of larvae of Strongyloides stercoralis and S. ratti respectively obtained from human and rat feces cultures, were used as antigens. Fluoresceinate conjugates against human IgG were employed at the ideal titer of 10 for S. stercoralis and 100 for S. ratti. The sensitivity of the indirect immunofluorescence reaction (IIF) was 94.4% and 92.5% and the specificity 94.2% and 97.1% for the two specific larval antigens, respectively. Sera from 123 persons (54 from carriers of S. stercoralis infections and 69 from controls) were submitted to the reaction. The titers of different sera varied from 20 to 2560. There was a significant linear correlation (r = 0.85 p < or = 0.001) between the antibodies from the two species of larval antigens. We conclude that both antigens may be used in the IIF reaction for the diagnosis of human strongyloidiasis. Due to the feasibility of safe and low-cost mass production of S. ratti larvae in the laboratory with a considerable economy of conjugate, their utilization in the serum diagnosis of human strongyloidiasis is recommended.
Departamento de Patologia, Universidade Federal de Uberlândia, MG, Brasil.
J M
JM
Costa-Cruz
C B
CB
Bullamah
M do R
Mdo R
Gonçalves-Pires
D M
DM
Campos
M A
MA
Vieira
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D016616:Cryoultramicrotomy; D005243:Feces / Q000469:parasitology; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007814:Larva / Q000276:immunology; D051381:Rats; D012680:Sensitivity and Specificity; D017170:Strongyloides ratti / Q000276:immunology*; D017171:Strongyloides stercoralis / Q000276:immunology*; D013322:Strongyloidiasis / Q000175:diagnosis*
D000947:Antigens, Helminth