Iron deficiency-induced tetracycline production in submerged cultures by Streptomyces aureofaciens.
Streptomyces aureofaciens ATCC 10762 grown in rotary-shaken submerged cultures produced substantial amounts of tetracycline only when the defined medium was deprived of iron. The biosynthesis of tetracycline was inhibited either by free iron at concentrations above 1-2 mumol l-1, or by chelated iron provided by the siderophores of this bacterial strain. Late static iron-containing cultures allowed cell differentiation and sporulation and led to tetracyclines synthesis. A nitrosoguanidine-induced mutant able to synthesize tetracycline in the presence of iron in shaken submerged cultures was isolated and compared to the wild-type strain. However, no constitutive siderophore-mediated iron transport occurred in the mutant. These results suggest the involvement of a putative iron-controlled repressor in the biosynthesis of these secondary metabolites during vegetative growth and primary metabolism of the bacterium.
Laboratoire de Microbiologie Fondamentale et Appliquée, Université des Sciences et Technologies de Lille, France.
M
M
Béchet
R
R
Blondeau
D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D017262:Siderophores / Q000096:biosynthesis; D013304:Streptomyces aureofaciens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013752:Tetracycline / Q000096:biosynthesis*
D017262:Siderophores; D007501:Iron; D013752:Tetracycline