Molecular cloning and transfection studies of M6b-2, a novel splice variant of a member of the PLP-DM20/M6 gene family.
The present study documents the nucleic acid and deduced amino acid sequence of M6b-2, a novel splice variant of the M6b gene, which belongs to the PLP-DM20/M6 gene family. M6b-2 differs from the previously published M6b by a novel 40-amino acid insertion which is characterised by a high proline content, two casein kinase, and one tyrosine kinase consensus sequences. M6b-2 mRNA is enriched in perinatal central nervous system (CNS), and although it declines during development, it does persist into adulthood. Transient transfection studies coupled to secondary structure and hydrophobicity analysis suggest that the novel polypeptide in M6b-2 lies at the cytoplasmic face of the plasma membrane. It is therefore possible that the function(s) of M6b-2 may be regulated intracellularly by phosphorylation during CNS development.
Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Scotland.
D A
DA
Vouyiouklis
H
H
Werner
I R
IR
Griffiths
G J
GJ
Stewart
K
K
Armin-Nave
C E
CE
Thomson
D000375:Aging / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D001921:Brain / Q000378:metabolism; D002460:Cell Line; D003001:Cloning, Molecular; D004274:DNA, Recombinant; D004285:Dogs; D014644:Genetic Variation; D008562:Membrane Glycoproteins / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D018991:Myelin Proteolipid Protein / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection
D004274:DNA, Recombinant; C083481:Gpm6b protein, mouse; D008562:Membrane Glycoproteins; D018991:Myelin Proteolipid Protein; D009419:Nerve Tissue Proteins; C047516:Plp1 protein, mouse; D012333:RNA, Messenger