Regulatory regions in the promoters of the Saccharomyces cerevisiae PYC1 and PYC2 genes encoding isoenzymes of pyruvate carboxylase.
We have identified regions in the promoters of the PYC1 and PYC2 genes from Saccharomyces cerevisiae involved in their regulation in different culture conditions. In the case of PYC1, a UAS in the region between -330/-297 and three repressing sequences with the common central core CCGCC at positions -457, -432 and -399 were identified. Specific binding of nuclear proteins to the -330/-214 DNA fragment was abolished in rtg mutants suggesting a role for the RTG genes in the control of PYC1 expression. In the case of the PYC2 promoter, elimination of a fragment from -417 to -291 brings about a two-fold decrease in the expression in repressed conditions and a similar increase in derepression.
Instituto de Investigaciones Biomédicas, C.S.I.C., Unidad de Bioquímica y Genética de Levaduras, Madrid, Spain.
J
J
Menéndez
C
C
Gancedo
D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D007527:Isoenzymes; D011401:Promoter Regions, Genetic; D011766:Pyruvate Carboxylase / Q000096:biosynthesis / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase; D011766:Pyruvate Carboxylase