Expression of rat gene for L-gulono-gamma-lactone oxidase, the key enzyme of L-ascorbic acid biosynthesis, in guinea pig cells and in teleost fish rainbow trout (Oncorhynchus mykiss).
The ability of rainbow trout liver and kidney preparations to produce L-ascorbic acid with an added source of L-gulono-gamma-lactone oxidase (GLO) and the absence of their own GLO activity suggested that the reason for the absence of L-ascorbic acid biosynthesis in fish and in guinea pig, a scurvy-prone mammal, can be similar. Nevertheless, results of rat GLO cDNA expression in guinea pig cells and in rainbow trout proved different. In guinea pig cells, rat GLO was expressed in a functional form. Regardless of recombinant GLO transcripts detected in rainbow trout embryos, alevins and in juvenile fish, neither GLO protein nor GLO activity were found. Furthermore, production of L-ascorbic acid in transgenic rainbow trout was not revealed in feeding tests with vitamin C-free diets or after direct administration of L-gulono-gamma-lactone. These results indicate that conditions required for translation or stability of rat GLO are absent in rainbow trout tissues.
Krasnov
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A
Department of Applied Zoology and Veterinary Medicine, University of Kuopio, P.O. Box 1627, 70211 Kuopio, Finland. krasnov@uku.fi
Reinisalo
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M
Pitkänen
T I
TI
Nishikimi
M
M
Mölsä
H
H
D000818:Animals; D030801:Animals, Genetically Modified; D001205:Ascorbic Acid / Q000096:biosynthesis*; D001206:Ascorbic Acid Deficiency / Q000378:metabolism; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004032:Diet; D015870:Gene Expression; D018014:Gene Transfer Techniques; D006168:Guinea Pigs; D050544:L-Gulonolactone Oxidase; D017686:Oncorhynchus mykiss / Q000235:genetics* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D012614:Scurvy / Q000209:etiology / Q000378:metabolism; D013045:Species Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics*
D017931:DNA Primers; D018076:DNA, Complementary; D013401:Sugar Alcohol Dehydrogenases; D050544:L-Gulonolactone Oxidase; D001205:Ascorbic Acid