Development of a magnetic microplate chemifluorimmunoassay for rapid detection of bacteria and toxin in blood.
A magnetic microplate chemifluorimmunoassay (MMCIA) is described using an immunomagnetic separation and a fluorescent microplate technique for rapid detection of low-level Escherichia coli O157:H7, Bacillus subtilis var. niger spores, and Staphylococcal enterotoxin type B from whole blood. In general, the MMCIA has at least several-fold more sensitivity than the conventional enzyme-linked immunosorbent assay. In addition, the assay sensitivities using direct fluorochrome label as the reporter, or alkaline phosphatase (AP) with various assay substrates, such as pNPP and AttoPhos, were assessed.
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Veridian Corporation, 5200 Springfield Pike, Suite 200, Dayton, OH 45431-1289, USA.
Ahmed
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Vasta
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D000818:Animals; D000907:Antibodies, Bacterial; D001412:Bacillus subtilis / Q000276:immunology / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D002623:Chemistry Techniques, Analytical; D004768:Enterotoxins / Q000097:blood / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D019453:Escherichia coli O157 / Q000276:immunology / Q000302:isolation & purification; D005456:Fluorescent Dyes; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D006041:Goats; D006801:Humans; D018189:Immunomagnetic Separation; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep; D013171:Spores, Bacterial / Q000276:immunology / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D004768:Enterotoxins; D005456:Fluorescent Dyes; C031818:enterotoxin B, staphylococcal