Intensity modulation of pseudocolor images.
Fura-2 is a popular real-time fluorescent indicator used to image intracellular, free calcium concentrations ([Ca2+]i) in living cells. One difficulty with fura-2-based digital imaging is how to best display the [Ca2+]i information that is acquired. Typically, only the [Ca2+]i as pseudocolor data are displayed, and the localization and intensity of the dye are not presented. This paper describes a technique that allows for the re-introduction of the intensity information. Recovery of the intensity information would de-emphasize regions with a poor signal-to-noise ratio and would more accurately report variations of sample morphology. A second advantage of this technique is that it requires no additional hardware since it is completely software based and can be run on all the major operating systems.
University of Minnesota, Minneapolis, USA.
L E
LE
Hinman
P J
PJ
Sammak
D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003116:Color / Q000592:standards*; D003118:Color Perception; D003626:Data Display; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D016257:Fura-2 / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted; D011650:Pulmonary Alveoli / Q000737:chemistry / Q000166:cytology / Q000187:drug effects; D051381:Rats
D005456:Fluorescent Dyes; D002118:Calcium; D016257:Fura-2