Orphan opioid receptor antisense probes block orphanin FQ-induced hyperphagia.
Orphanin FQ/nociceptin binds with high affinity to the orphan opioid receptor-like/K-3 (ORL1/KOR-3) clone, and stimulates feeding. The present study demonstrated that antisense oligodeoxynucleotides directed against either exons 1, 2 or 3 of the ORL1/KOR-3 clone reduced orphanin FQ/nociceptin-induced hyperphagia. A missense probe was ineffective. Naltrexone dose-dependently reduced orphanin FQ/nociceptin-induced hyperphagia. These data suggest that the receptor responsible for orphanin FQ/nociceptin-induced hyperphagia is encoded by the ORL1/KOR-3 clone.
Leventhal
L
L
Department of Psychology, Queens College, City University of New York, Flushing 11367, USA.
Mathis
J P
JP
Rossi
G C
GC
Pasternak
G W
GW
Bodnar
R J
RJ
D000818:Animals; D004435:Eating / Q000187:drug effects; D005091:Exons; D006963:Hyperphagia / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D009271:Naltrexone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D018847:Opioid Peptides / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000235:genetics / Q000502:physiology*; D000094942:Nociceptin Receptor; D000097629:Nociceptin
D009271:Naltrexone; D009292:Narcotic Antagonists; D016376:Oligonucleotides, Antisense; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000094942:Nociceptin Receptor; D000097629:Nociceptin; C000722285:Oprl1 protein, rat