Brown-midrib maize (bm1)--a mutation affecting the cinnamyl alcohol dehydrogenase gene.
Brown-midrib (bm) mutants of maize have modified lignin of reddish-brown colour. Although four independent bm loci are known, only one of the mutant genes has been previously identified. We report here that maize bm1, one of the less characterised mutants, shows severely reduced CAD activity in lignified tissues, resulting in the production of a modified lignin. Both the total lignin content and the structure of the polymer are altered by the mutation. We further describe the isolation and characterisation of the maize CAD cDNA and mapping of the CAD gene. CAD maps very closely to the known location of bm1 and co-segregates with the bm1 locus in two independent recombinant inbred populations. These data strongly support the premise that maize bm1 directly affects expression of the CAD gene.
Halpin
C
C
Zeneca Seeds, Jealott's Hill Research Station, Bracknell, Berks, UK. c.halpin@dundee.ac.uk
Holt
K
K
Chojecki
J
J
Oliver
D
D
Chabbert
B
B
Monties
B
B
Edwards
K
K
Barakate
A
A
Foxon
G A
GA
D000429:Alcohol Oxidoreductases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003116:Color; D018076:DNA, Complementary; D008031:Lignin / Q000096:biosynthesis / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics*
D018076:DNA, Complementary; D015345:Oligonucleotide Probes; D011994:Recombinant Proteins; D008031:Lignin; D000429:Alcohol Oxidoreductases; C018656:cinnamyl alcohol dehydrogenase