Increased expression of 3-hydroxyanthranilate 3,4-dioxygenase gene in brain of epilepsy-prone El mice.
The El mouse is an established animal model for human epilepsy. We previously reported that the level of quinolinic acid (QUIN), an excitotoxin, was high in the brain of epilepsy-prone El mice and that the increased production of QUIN was secondary to an increased activity of 3-hydroxyanthranilate 3,4-dioxygenase (3-HAO, EC 1.13.11. 6) in the brains of these mice. In this study, we cloned and sequenced the cDNA for 3-HAO and showed that its expression in the brain of El mice was higher than that of control ddY mice. These results suggest that a genetic defect leading to derepression of the 3-HAO gene expression in the brain may be involved in the pathogenesis for the epileptic diseases of El mice.
Nagoya University Bioscience Center, Chikusa, Nagoya 464-8601, Japan.
Y
Y
Nakagawa
H
H
Asai
T
T
Miura
J
J
Kitoh
H
H
Mori
K
K
Nakano
D050561:3-Hydroxyanthranilate 3,4-Dioxygenase; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D049308:Dioxygenases; D004195:Disease Models, Animal; D004827:Epilepsy / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D051379:Mice; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D016376:Oligonucleotides, Antisense; D016366:Open Reading Frames; D010105:Oxygenases / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010446:Peptide Fragments / Q000737:chemistry; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012016:Reference Values
D017931:DNA Primers; D018076:DNA, Complementary; D016376:Oligonucleotides, Antisense; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D010105:Oxygenases; D049308:Dioxygenases; D050561:3-Hydroxyanthranilate 3,4-Dioxygenase