A novel oestrogen-regulated gene in human breast cancer cells identified by differential display.
Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859 of the published sequence for the mRNA for human megakaryocyte CD63 antigen but has a 3' tail extended by 23 nucleotides. Northern blotting has confirmed that this mRNA is regulated by oestrogen in both MCF7 and T47D human breast cancer cell lines.
Division of Cell and Molecular Biology, School of Animal and Microbial Sciences, The University of Reading, Whiteknights, UK.
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Stephen
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Corcoran
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Darbre
D015703:Antigens, CD / Q000235:genetics*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005821:Genetic Techniques; D006801:Humans; D008969:Molecular Sequence Data; D010980:Platelet Membrane Glycoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D060149:Tetraspanin 30; D014158:Transcription, Genetic / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; C554586:CD63 protein, human; D018076:DNA, Complementary; D004967:Estrogens; D010980:Platelet Membrane Glycoproteins; D012333:RNA, Messenger; D060149:Tetraspanin 30