Inhibition of NMDA receptors and nitric oxide synthase reduces ischemic injury of the retina.
This study was performed to examine the roles of body temperature, NMDA receptors and nitric oxide (NO) synthase in post-ischemic retinal injury in rats. Cell loss in the ganglion cell layer and thinning of the inner plexiform layer were observed 7 days after ischemia. Cell loss in the ganglion cell layer but not thinning of the inner plexiform layer was reduced by hypothermia during ischemia. Intravenous injection of dizocilpine (MK-801) or Nomega-nitro-L-arginine methyl ester (L-NAME) prior to ischemia ameliorated retinal injury. These results suggest that activation of NO synthase following NMDA receptor stimulation is involved in ischemia-induced retinal injury.
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
K
K
Adachi
Y
Y
Fujita
C
C
Morizane
A
A
Akaike
M
M
Ueda
M
M
Satoh
H
H
Masai
S
S
Kashii
Y
Y
Honda
D000818:Animals; D001831:Body Temperature / Q000187:drug effects; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007511:Ischemia; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000037:antagonists & inhibitors / Q000502:physiology*; D012160:Retina / Q000378:metabolism; D012164:Retinal Diseases / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control; D012171:Retinal Vessels
D004791:Enzyme Inhibitors; D018696:Neuroprotective Agents; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester