Complex induction of bovine uterine proteins by interferon-tau.
Interferon-tau (IFN-tau) is released by the conceptus and induces two uterine proteins during early pregnancy: ubiquitin cross-reactive protein (UCRP) and granulocyte chemotactic protein-2 (GCP-2). The present experiments were designed to determine whether detection (Western blot) of cytosolic UCRP and release of GCP-2 could be used to examine IFN-tau signal transduction in cultured endometrial explants and primary epithelial cells. Recombinant (r) type 1 IFNs (rboIFN-tau and rboIFN-alpha; 5, 25, 100 nM) induced UCRP, but only rboIFN-tau induced GCP-2 in explant culture. Recombinant boIFN-tau and conceptus secretory proteins containing native IFN-tau induced UCRP and GCP-2 in cultured primary epithelial cells. All concentrations of rboIFN-alpha (25, 50, 100 nM) induced UCRP, but only the highest concentration induced GCP-2 in cultured primary epithelial cells. Interestingly, phorbol ester (100, 500, 1000 ng/ml) induced GCP-2, but it had no effect on UCRP. Because type 1 IFNs induce UCRP, IFN-tau probably interacts with the janus kinase (Jak)-associated IFN-alpha receptor to phosphorylate signal transducers and activators of transcription (STAT) and/or interferon regulatory factor-1 (IRF-1). However, IFN-tau-specific induction of GCP-2 may involve a variant type 1 receptor subunit or activators of transcription that are associated with protein kinase C and the Jak/STAT/IRF-1 pathway.
Staggs
K L
KL
Department of Animal Science, University of Wyoming, Laramie, Wyoming 82071, USA.
Austin
K J
KJ
Johnson
G A
GA
Teixeira
M G
MG
Talbott
C T
CT
Dooley
V A
VA
Hansen
T R
TR
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D002478:Cells, Cultured; D054427:Chemokine CXCL6; D019743:Chemokines, CXC / Q000096:biosynthesis; D002846:Chromatography, Affinity; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism; D004847:Epithelial Cells; D004971:Estrus / Q000187:drug effects / Q000502:physiology; D005260:Female; D007370:Interferon Type I / Q000494:pharmacology*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011994:Recombinant Proteins; D013268:Stimulation, Chemical; D014452:Ubiquitins / Q000096:biosynthesis; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D054427:Chemokine CXCL6; D019743:Chemokines, CXC; D007370:Interferon Type I; D011257:Pregnancy Proteins; D011994:Recombinant Proteins; D014452:Ubiquitins; C020947:interferon tau