Light affects c-fos expression and phospholipid synthesis in both retinal ganglion cells and photoreceptor cells in an opposite way for each cell type.
Retina photoreceptor and ganglion cells isolated from chicks that in vivo were exposed to light have a different phospholipid labeling capacity than those from chicks in the dark. In the light exposed animals, the phospholipid labeling in the ganglion cells is higher (Delta% 45, p<0.005) than in those maintained in the dark, whereas in the photoreceptor cells, the opposite occurs, that is, the phospholipid labeling is higher in the dark than in light. The light-dark differences for phospholipid labeling correlate with the expression of c-fos: when c-fos expression increases (both in mRNA and in c-Fos protein content), phospholipid labeling increases concomitantly. That is, in ganglion cells, c-fos expression and the phospholipid synthesis is higher in light with respect to dark, whereas in photoreceptor cells, c-fos expression and phospholipid synthesis is higher in dark with respect to light. Moreover, when an oligonucleotide antisense to c-fos is administered intraocularly prior to separating the animals into light and dark, no differences in c-fos expression and, consequently, no differences in phospholipid synthesis are found between animals in light and dark. Taken together, these results point to a novel mechanism by which rapid genomic responses to cell stimulation are converted to longer lasting changes in the cell components.
Bussolino
D F
DF
CIQUIBIC (CONICET) Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina.
de Arriba Zerpa
G A
GA
Grabois
V R
VR
Conde
C B
CB
Guido
M E
ME
Caputto
B L
BL
D000818:Animals; D002645:Chickens; D003624:Darkness; D005786:Gene Expression Regulation / Q000528:radiation effects; D016762:Genes, fos / Q000528:radiation effects*; D007150:Immunohistochemistry; D008027:Light; D010743:Phospholipids / Q000096:biosynthesis*; D010786:Photoreceptor Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis*; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D014158:Transcription, Genetic / Q000528:radiation effects
D010743:Phospholipids; D016760:Proto-Oncogene Proteins c-fos