Multicellular stalk-like structures in Saccharomyces cerevisiae.
Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproducibly in large numbers. Yeast mutants, mutated for control of cell polarity, developmental processes, UV response, and signal transduction cascades were tested and found capable of forming stalk-like structures. We suggest a model that explains the mechanism of stalk formation by mechanical environmental forces. We show that other microorganisms (Candida albicans, Schizosaccharomyces pombe, and Escherichia coli) also form stalks, suggesting that the ability to produce stalks may be a general property of microorganisms. Diploid yeast stalks sporulate at an elevated frequency, raising the possibility that the physiological role of stalks might be disseminating spores.
Engelberg
D
D
Department of Biological Chemistry, The Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel. ENGELBER@vms.huji.ac.il
Mimran
A
A
Martinetto
H
H
Otto
J
J
Simchen
G
G
Karin
M
M
Fink
G R
GR
D002176:Candida albicans / Q000648:ultrastructure; D002462:Cell Membrane / Q000528:radiation effects / Q000648:ultrastructure; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000502:physiology* / Q000528:radiation effects / Q000648:ultrastructure*; D012568:Schizosaccharomyces / Q000648:ultrastructure; D015398:Signal Transduction; D013045:Species Specificity; D013172:Spores, Fungal; D014466:Ultraviolet Rays