Characterization of ADG1, an Arabidopsis locus encoding for ADPG pyrophosphorylase small subunit, demonstrates that the presence of the small subunit is required for large subunit stability.
Two mutants of Arabidopsis have been isolated that affect ADPG pyrophosphorylase (ADGase) activity. Previously, it has been shown that ADG2 encodes the large subunit of ADGase. This study characterizes the adg1 mutant phenotype and ADG1 gene structure. RNA blot analyses indicate that the adg1-1 mutant accumulates transcripts encoding both the large and small subunits of ADGase, while the adg1-2 mutant accumulates only large subunit transcripts. RFLP analysis and complementation of adg1 mutants with the ADGase small subunit gene demonstrate that ADG1 encodes the small subunit. Sequence analysis indicates that adg1-1 represents a missense mutation within the gene. Western blot analysis confirms that adg1 mutants contain neither the large nor the small subunit proteins, suggesting that the presence of functional small subunits is required for large subunit stability.
Department of Botany, National Taiwan University, Taipei, Republic of China.
S M
SM
Wang
W L
WL
Lue
T S
TS
Yu
J H
JH
Long
C N
CN
Wang
K
K
Eimert
J
J
Chen
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D017343:Genes, Plant; D005816:Genetic Complementation Test; D051599:Glucose-1-Phosphate Adenylyltransferase; D008969:Molecular Sequence Data; D009154:Mutation; D009713:Nucleotidyltransferases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D010641:Phenotype; D012150:Polymorphism, Restriction Fragment Length; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D013213:Starch / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D018744:DNA, Plant; D011994:Recombinant Proteins; D013213:Starch; D009713:Nucleotidyltransferases; C496715:ADP-glucose pyrophosphorylase, Arabidopsis; D051599:Glucose-1-Phosphate Adenylyltransferase