c-Myc-associated genomic instability of the dihydrofolate reductase locus in vivo.
c-Myc overexpression is associated with the locus-specific amplification and rearrangement of the dihydrofolate reductase (DHFR) gene. This has been shown in lymphoid and nonlymphoid cell lines. Furthermore, c-Myc-dependent DHFR gene amplification occurs independent of species origins; it has been described in rat, hamster, mouse, and human cell lines. Here, we report on c-Myc-dependent amplification of the DHFR gene in vivo, using an animal model of c-Myc-dependent neoplasia, the mouse plasmacytoma.
Manitoba Institute of Cell Biology and the University of Manitoba, Winnipeg, Canada.
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Taylor
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Mai
D000818:Animals; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D004195:Disease Models, Animal; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008807:Mice, Inbred BALB C; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000378:metabolism; D010954:Plasmacytoma / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D016271:Proto-Oncogene Proteins c-myc; D013762:Tetrahydrofolate Dehydrogenase