Oncogene and tumour suppressor gene products during trophoblast differentiation in the first trimester.
Tumour suppressor genes may have a role in the control of trophoblast cell population expansion as trophoblast invasion occurs. To investigate this hypothesis, the location of tumour suppressor gene and proto-oncogene products were studied at various stages of trophoblast differentiation and invasion. Trophoblast and decidua were obtained from eight women having a therapeutic termination of pregnancy. Immunohistochemistry was used to localize the products of c-myc, c-erB-2, RB, BCL-2, P21, and P53 genes and anti-cytokeratin was used to identify fetal cells amongst the maternal decidual cells. The most differentiated and furthest invading trophoblast cell type, the multinucleated trophoblast, expressed a combination of genes which may indicate a high apoptotic rate. The other fully differentiated trophoblast, the syncytiotrophoblast, expressed BCL-2 suggesting protection from apoptosis. The co-occurrence of proto-oncogenes and the products of tumour suppressor genes in first trimester trophoblast suggests an important role not only in negative regulation of cellular invasion but also in population expansion through the presence of oncogenes and anti-apoptotic proteins.
Department of Obstetrics and Gynaecology, University of Liverpool, UK.
S
S
Quenby
C
C
Brazeau
A
A
Drakeley
D I
DI
Lewis-Jones
G
G
Vince
D017209:Apoptosis / Q000235:genetics; D002454:Cell Differentiation; D004249:DNA Damage; D003656:Decidua / Q000166:cytology / Q000378:metabolism; D005260:Female; D005326:Fetal Proteins / Q000032:analysis; D018507:Gene Expression Regulation, Developmental; D016147:Genes, Tumor Suppressor; D006801:Humans; D007633:Keratins / Q000032:analysis; D009857:Oncogenes; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis* / Q000235:genetics; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*
D005326:Fetal Proteins; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D007633:Keratins