Iron deficiency-induced tetracycline production in submerged cultures by Streptomyces aureofaciens.
Streptomyces aureofaciens ATCC 10762 grown in rotary-shaken submerged cultures produced substantial amounts of tetracycline only when the defined medium was deprived of iron. The biosynthesis of tetracycline was inhibited either by free iron at concentrations above 1-2 mumol l-1, or by chelated iron provided by the siderophores of this bacterial strain. Late static iron-containing cultures allowed cell differentiation and sporulation and led to tetracyclines synthesis. A nitrosoguanidine-induced mutant able to synthesize tetracycline in the presence of iron in shaken submerged cultures was isolated and compared to the wild-type strain. However, no constitutive siderophore-mediated iron transport occurred in the mutant. These results suggest the involvement of a putative iron-controlled repressor in the biosynthesis of these secondary metabolites during vegetative growth and primary metabolism of the bacterium.
Béchet
M
M
Laboratoire de Microbiologie Fondamentale et Appliquée, Université des Sciences et Technologies de Lille, France.
Blondeau
R
R
D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D017262:Siderophores / Q000096:biosynthesis; D013304:Streptomyces aureofaciens / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013752:Tetracycline / Q000096:biosynthesis*
D017262:Siderophores; D007501:Iron; D013752:Tetracycline