Direct fractionation of proteins in particle-containing feedstocks by a filter paper pieces-based DEAE-cellulose column chromatography. Rapid, robust and low-cost capturing procedure for protein.
Filter paper pieces-based (FPB) DEAE-celluloses was prepared for direct fractionation of proteins in particle-containing feedstocks. FPB DEAE-cellulose has a protein binding capacity equivalent to that of commercially available DEAE-cellulose. Crude extracts from porcine intestine and kiwi fruit pulp, which were unmanageable by commercially available chromatographic media due to rapid clothing, could be directly fractionated with FPB DEAE-cellulose column. In addition, effluents from an FPB DEAE-cellulose column were extensively clarified. The present approach can be used as a rapid, robust and low-cost capturing step for protein from particle-containing feedstocks.
Department of Food Sciences and Nutritional Health, Kyoto Prefectural University, Japan.
K
K
Sato
Y H
YH
Guo
J
J
Feng
S
S
Sugiyama
M
M
Ichinomiya
Y
Y
Tsukamasa
Y
Y
Minegishi
A
A
Sakata
K
K
Komiya
Y
Y
Yamasaki
Y
Y
Nakamura
K
K
Ohtsuki
M
M
Kawabata
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D003365:Costs and Cost Analysis; D004044:Dietary Proteins / Q000032:analysis*; D005374:Filtration; D005504:Food Analysis; D005638:Fruit / Q000737:chemistry; D008460:Meat / Q000032:analysis; D015203:Reproducibility of Results; D013552:Swine; D014783:Viscosity
D004044:Dietary Proteins