A novel complex of proteins binds the HIV-1 promoter upon virus interaction with CD4.
HIV-1Lai13EM is a mutant isolate which is less sensitive than the parental HIV-1Lai strain to an in vitro treatment with 13B8-2 anti-CD4 monoclonal antibody (mAb) that generally inhibits transcription of HIV-1 and HIV-2. In contrast to other clade B viruses, this isolate carries a point mutation G > A at position -188 of the viral promoter. The fact that HIV-1NDK, a clade D virus insensitive to 13B8-2 mAb, also carries an A nucleotide at this position has brought our attention to the sequence surrounding position -188. Here we analyzed whether a DNA-binding molecule interacts with this region. Electrophoretic mobility shift assays performed with the -201/-175 HIV-1Lai wild-type sequence or the sequence containing a point mutation G > A at position -188 demonstrated their ability to bind a heterotrimeric complex induced in CEM cells by stimulation with heat-inactivated HIV-1.
CRBM/CNRS UPR 1086, Laboratoire Infections Rétrovirales et Signalisation Cellulaire, Institut de Biologie, Montpellier, France.
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Coudronnière
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Devaux
D000225:Adenine; D000911:Antibodies, Monoclonal; D001483:Base Sequence; D001665:Binding Sites; D015704:CD4 Antigens / Q000378:metabolism*; D002460:Cell Line; D016384:Consensus Sequence; D006151:Guanosine; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016328:NF-kappa B / Q000378:metabolism; D017354:Point Mutation; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D016415:Sequence Alignment; D014158:Transcription, Genetic; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016328:NF-kappa B; D006151:Guanosine; D000225:Adenine