The structure and function of the PQQ-containing quinoprotein dehydrogenases.
Bacterial methanol and glucose dehydrogenases containing a novel type of prosthetic group, subsequently identified as pyrrolo-quinoline quinone (PQQ), were first described about 30 years ago. Quinoproteins were originally defined as proteins containing PQQ but this definition has since been broadened to include those proteins containing other types of quinone-containing prosthetic groups, and the X-ray structures of representatives of each type of quinoprotein have recently been published. This review is mainly concerned with the structure and function of the PQQ-containing methanol dehydrogenase, whose structure has been determined at high resolution, and related proteins. Their basic structure consists of a 'propeller' fold superbarrel made up of 8-sheet 'propeller blades' which are held together by novel tryptophan-docking motifs. In methanol dehydrogenase the PQQ in the active site is coordinated to a Ca2+ ion and is maintained in position by a stacked tryptophan and a novel 8-membered ring structure made up of a disulphide bridge between adjacent cysteine residues. This review describes these features and discusses them in relation to previously proposed mechanisms for this enzyme.
Anthony
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Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, U.K. c.anthony@soton.ac.uk
Ghosh
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D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000378:metabolism*; D001665:Binding Sites; D003067:Coenzymes / Q000032:analysis / Q000737:chemistry*; D008958:Models, Molecular; D045542:PQQ Cofactor; D017433:Protein Structure, Secondary; D015363:Quinolones / Q000032:analysis / Q000737:chemistry*; D011809:Quinones / Q000032:analysis / Q000737:chemistry*
D003067:Coenzymes; D015363:Quinolones; D011809:Quinones; D045542:PQQ Cofactor; D000429:Alcohol Oxidoreductases; C019641:alcohol dehydrogenase (acceptor)