Auxiliary heterotopic liver transplantation in the rat: a simplified model using cuff technique and application for congenitally hyperbilirubimemic Gunn rat.
To study the immunological and metabolic effects of auxiliary liver transplantation (ALT), a simple ALT model in rats was developed using the cuff application. Effects of transient parking of normal liver were tested in congenitally hyperbilirubinemic Gunn rats. Serum bilirubin concentrations in Gunn rats, transplanted heterotopically with normal livers of Wistar rats, were dramatically reduced and maintained within normal levels. The graftectomy was performed safely 1 month after transplantation, but total bilirubin levels did not return to the preoperative value of the Gunn rats. It is possible that hepatic stem cells included in ALT liver migrated to the host liver and differentiated into cells capable of producing certain enzymes.
Kobayashi
E
E
Department of Surgery, Meikai University, Sakado, Saitama, Japan. eijikoba@jichi.ac.jp
Yoshida
Y
Y
Nozawa
M
M
Hishikawa
S
S
Yamanaka
T
T
Miyata
M
M
Fujimura
A
A
D000704:Analysis of Variance; D000818:Animals; D001663:Bilirubin / Q000097:blood* / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival; D006933:Hyperbilirubinemia, Hereditary / Q000601:surgery; D008099:Liver / Q000378:metabolism*; D016031:Liver Transplantation / Q000276:immunology / Q000379:methods*; D008297:Male; D051381:Rats; D011911:Rats, Gunn; D017208:Rats, Wistar; D013045:Species Specificity; D016042:Transplantation, Heterotopic / Q000276:immunology / Q000379:methods*
D001663:Bilirubin