Novel combretastatin analogues effective against murine solid tumors: design and structure-activity relationships.
A series of combretastatin A-4 (CA-4) analogues were synthesized, and their cytotoxic effects against murine Colon 26 adenocarcinoma and inhibitory activity on tubulin polymerization were evaluated. Since CA-4 has limited aqueous solubility, the target compounds were designed to improve solubility by introduction of a nitrogen-containing group. Among the compounds synthesized, those with an amino moiety in place of the phenolic OH of CA-4 showed potent antitubulin activity and cytotoxicity against murine Colon 26 adenocarcinoma in vitro. Some of the compounds which were potent in vitro were evaluated in the murine tumor model Colon 26 in vivo. Among these, 13bHCl, 21aHCl, and 21bHCl showed significant antitumor activity in the animal model, while CA-4 was ineffective. 13bHCl and 21aHCl were further evaluated in two murine tumor models (Colon 38 and 3LL) and human xenografts HCT-15. These compounds showed potent antitumor activity comparable or superior to that of CDDP. The structure-activity relationships of this series of compounds are also discussed.
Ohsumi
K
K
Central Research Laboratories, Ajinomoto Company Ltd., 1-1 Suzuki-cho, Kawasaki, Kawasaki 210, Japan.
Nakagawa
R
R
Fukuda
Y
Y
Hatanaka
T
T
Morinaga
Y
Y
Nihei
Y
Y
Ohishi
K
K
Suga
Y
Y
Akiyama
Y
Y
Tsuji
T
T
D000181:Acrylonitrile / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000230:Adenocarcinoma / Q000473:pathology; D000814:Aniline Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000840:Anisoles / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D001704:Biopolymers; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000473:pathology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D051379:Mice; D009368:Neoplasm Transplantation; D012995:Solubility; D013267:Stilbenes / Q000737:chemistry / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014183:Transplantation, Heterologous; D014404:Tubulin / Q000378:metabolism; D014407:Tumor Cells, Cultured
C113645:2-methyl-5-(2-(3,4,5-trimethoxyphenyl)vinyl)phenylamine; C113644:3-(3-amino-4-methoxyphenyl)-2-(3,4,5-trimethoxyphenyl)acrylonitrile; D000814:Aniline Compounds; D000840:Anisoles; D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; D001704:Biopolymers; D013267:Stilbenes; D014404:Tubulin; C058728:fosbretabulin; D000181:Acrylonitrile