Cationic multilamellar liposome-mediated human interferon-beta gene transfer into cervical cancer cell.
Interferons (IFNs) have antineoplastic activity, but it has been reported that treatment with IFN alone is not effective in many cancers. To enhance the effect of growth inhibition on tumor cells by raising the concentration, we attempted the transfection of cervical cancer cells, HeLa cells, with human interferon-beta (HuIFN-beta) cDNA contained in the expression vector pRSV delivered by cationic multilamellar liposomes, which resulted in the secretion of HuIFN-beta into the medium. The concentration of HuIFN-beta in the medium was 22 IU/ml by 72 hours after transfection of 10 ng DNA, and provoked around 45-fold cell growth inhibitory effect compared with that of exogenously added HuIFN-beta (1000 IU/ml). This strong growth inhibition was considered to be due to the action of HuIFN-beta in a paracrine manner, and a notable fraction of the cell death was apoptotic.
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
K
K
Tomoda
N
N
Ohishi
F
F
Kikkawa
S
S
Mizutani
Y
Y
Tomoda
K
K
Yagi
D017209:Apoptosis; D001358:Avian Sarcoma Viruses; D002455:Cell Division; D053938:DNA Fragmentation; D018076:DNA, Complementary / Q000008:administration & dosage; D004337:Drug Carriers; D005260:Female; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D016899:Interferon-beta / Q000096:biosynthesis*; D008081:Liposomes; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection / Q000379:methods*; D002583:Uterine Cervical Neoplasms
D018076:DNA, Complementary; D004337:Drug Carriers; D008081:Liposomes; D011994:Recombinant Proteins; D016899:Interferon-beta