Citrinin-induced mitochondrial permeability transition.
The effects of mycotoxin citrinin on Ca2+ efflux and membrane permeabilization were studied in isolated rat liver mitochondria. The efflux rate observed when in presence of ruthenium red was higher when citrinin was added. Swelling experiments demonstrated Ca(2+)-dependent membrane permeabilization by citrinin. Catalase, butylhydroxitoluene (BHT), and dithiothreitol (DTT) did not protect swelling caused by Ca2+ plus citrinin. The protection conferred by ATP-Mg2+ and cyclosporin A in the latter experiments are strong indications of pore formation. These results suggest that citrinin can induce permeability transition by a mechanism that does not involve oxidative damage.
Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brasil.
E J
EJ
Da Lozzo
M B
MB
Oliveira
E G
EG
Carnieri
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics / Q000633:toxicity*; D000975:Antioxidants / Q000494:pharmacology; D001692:Biological Transport / Q000187:drug effects; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology; D002953:Citrinin / Q000493:pharmacokinetics / Q000633:toxicity*; D016572:Cyclosporine / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D007425:Intracellular Membranes / Q000187:drug effects; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008933:Mitochondrial Swelling / Q000187:drug effects*; D010539:Permeability / Q000187:drug effects; D051381:Rats; D012430:Ruthenium Red; D013170:Spores; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D013439:Sulfhydryl Reagents; D012430:Ruthenium Red; D002084:Butylated Hydroxytoluene; D002953:Citrinin; D016572:Cyclosporine; D002374:Catalase; D002118:Calcium; D004229:Dithiothreitol