Cloning and expression analyses of AtMRP4, a novel MRP-like gene from Arabidopsis thaliana.
In all organisms glutathione-conjugate transporters (GS-X pumps) mediate the detoxification of a number of xenobiotics by removing them from the cytosol. In addition, GS-X pumps appear to play a role in the processing of endogenous compounds. We have isolated a novel genomic clone from Arabidopsis thaliana that encodes a putative GS-X pump, AtMRP4, which is part of a recently defined gene family. The derived amino acid sequence shares high levels of similarity (55-63%) with human, yeast, and other Arabidopsis homologues. The expression of the different members of the AtMRP gene family in Arabidopsis cell suspensions after treatment with chemicals that modify glutathione metabolism (compounds that induce different types of stress and that act as herbicide antidotes- safeners- in monocotyledonous species) revealed that the members of this gene family are differentially regulated.
Sánchez-Fernández
R
R
Department of Genetics, Flanders Interuniversity Institute of Biotechnology, Universiteit Gent, Belgium.
Ardiles-Díaz
W
W
Van Montagu
M
M
Inzé
D
D
May
M J
MJ
D000595:Amino Acid Sequence; D000931:Antidotes / Q000494:pharmacology; D017360:Arabidopsis / Q000235:genetics*; D002104:Cadmium / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000502:physiology*; D017343:Genes, Plant / Q000235:genetics; D005978:Glutathione / Q000378:metabolism; D006540:Herbicides / Q000494:pharmacology; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D018384:Oxidative Stress; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D018749:RNA, Plant / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000931:Antidotes; D002352:Carrier Proteins; D006540:Herbicides; D026901:Membrane Transport Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; C086860:glutathione transporter; D002104:Cadmium; D005978:Glutathione