Proscillaridin A immunoreactivity: its purification, transport in blood by a specific binding protein and its correlation with blood pressure.
A material crossreacting with antibodies against the bufadienolide proscillaridin A and inhibiting the sodium pump was found in human blood plasma. The concentration of the material with a retention time similar to ouabain in a reversed phase HPLC correlated to systolic blood pressure and pulse pressure. Affinity purification of this compound from bovine adrenals resulted in the isolation of a compound with molecular mass of 600 Da that was not identical with ouabain. Consistent with the postulate that endogenous ouabain and proscillaridin A immunoreactivities may belong to a new class of cardiotonic steroid hormones, a protein of Mr = 60 kDa has been found in bovine serum by affinity-labeling with N-hydroxysuccimidyl digoxigenin-3-O-methylcarbonyl-epsilon-aminocaproate.
Institut für Biochemie & Endokrinologie, Justus-Liebig-Universität, Giessen, Germany.
R
R
Schneider
R
R
Antolovic
H
H
Kost
B
B
Sich
U
U
Kirch
M
M
Tepel
W
W
Zidek
W
W
Schoner
D000311:Adrenal Glands / Q000737:chemistry*; D000818:Animals; D001693:Biological Transport, Active; D001794:Blood Pressure / Q000502:physiology*; D002301:Cardiac Glycosides / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D003429:Cross Reactions / Q000276:immunology; D004791:Enzyme Inhibitors / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D011442:Proscillaridin / Q000276:immunology*; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D002301:Cardiac Glycosides; D004791:Enzyme Inhibitors; C028996:cardiac glycoside receptors; D000254:Sodium-Potassium-Exchanging ATPase; D011442:Proscillaridin