Practicable approaches to targeted comparative mapping of rat chromosome regions: linkage mapping of five genes on rat chromosome 13.
We demonstrate feasible approaches to comparative mapping between the region near the renin locus on rat chromosome 13 and human chromosome 1q by assigning five additional genes as anchor loci. Base-substitution polymorphisms were sought in the 3'- and/or 5'-untranslated regions for subsequent development of PCR-amplified markers, which, in turn, could be used for either restriction fragment analysis or allele-specific PCR.
The Wellcome Trust Centre for Human Genetics, Oxford University, Oxford (UK). kato@helios.jinkan.kyoto-u.ac.jp
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N
Kato
G
G
Hyne
J P
JP
Rapp
G M
GM
Lathrop
D000818:Animals; D015220:Calcium Channels / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D005260:Female; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000235:genetics; D006801:Humans; D007797:Laminin / Q000235:genetics; D008297:Male; D018008:Myogenin / Q000235:genetics; D011110:Polymorphism, Genetic; D051381:Rats; D011919:Rats, Inbred Strains; D011921:Rats, Inbred WKY; D012083:Renin / Q000235:genetics; D017422:Sequence Analysis, DNA; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics; D013045:Species Specificity
D015220:Calcium Channels; D006649:Histocompatibility Antigens; D007797:Laminin; C503799:MYOG protein, human; C503803:Myog protein, rat; D018008:Myogenin; C089141:laminin gamma 1; D012083:Renin; D000254:Sodium-Potassium-Exchanging ATPase