The recovering effect of betaine on carbon tetrachloride-induced liver injury.
The recovering effect of betaine (derivative of choline) on carbon tetrachloride (CCl4)-injured liver was investigated by oral and intraperitoneal administration of betaine at 24 h after acute CCl4 intoxication. The effect of betaine was estimated by the activity of alanine aminotransferase (ALT) released into the serum, histological score, and the incorporation of S-phase indicator (5-bromo-2-deoxyuridine/5-fluoro-2'-deoxyuridine; BrdU/FdU). A significant decrease in the activity of serum ALT was observed by the intraperitoneal (3 mg/kg body) or oral (15 mg/kg body) administration of betaine. Furthermore, an increase in the uptake of BrdU into the hepatocyte nuclear DNA and a reduction in liver necrosis after the oral treatment of betaine (15 mg/kg body) was observed. From these results, the administration of betaine showed a significant effect in recovering CCl4-injured liver.
Department of Pharmaceutics, Gifu Pharmaceutical University, Japan.
T
T
Murakami
Y
Y
Nagamura
K
K
Hirano
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001622:Betaine / Q000008:administration & dosage / Q000627:therapeutic use*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D002467:Cell Nucleus / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury; D004247:DNA / Q000096:biosynthesis; D008099:Liver / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008107:Liver Diseases / Q000188:drug therapy* / Q000201:enzymology; D008297:Male; D051379:Mice; D009336:Necrosis
D001622:Betaine; D004247:DNA; D002251:Carbon Tetrachloride; D000410:Alanine Transaminase