cDNA cloning of luteinizing hormone subunits from brushtail possum and red kangaroo.
Luteinizing hormone (LH) plays an important role in the reproductive cycles of all mammals. There is a large amount of both nucleotide and amino acid sequence data available for LH from eutherian mammals, but little is known about the primary structure of LH in marsupials. We have used consensus PCR primers to generate specific probes for screening pituitary cDNA libraries and report the cloning of the cDNAs encoding the alpha-subunit of LH (also shared by a number of other glycoprotein hormones) and the LH-specific beta-subunit, from the common brushtail possum, Trichosurus vulpecula, and the red kangaroo, Macropus rufus. Southern blotting experiments indicated that both genes are probably present as single copies. Comparison of the deduced marsupial protein sequences with homologous sequences from other vertebrates revealed a high degree of conservation, especially for the alpha-subunit. These sequences represent the first complete primary structures for a marsupial glycoprotein hormone to have been elucidated.
School of Science, University of Western Sydney, Nepean, PO Box 10, Kingswood, N.S.W., 2747, Australia.
G A
GA
Harrison
E M
EM
Deane
D W
DW
Cooper
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D007986:Luteinizing Hormone / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D007614:Macropodidae / Q000235:genetics*; D008969:Molecular Sequence Data; D009893:Opossums / Q000235:genetics*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014714:Vertebrates / Q000235:genetics
D017931:DNA Primers; D018076:DNA, Complementary; D007986:Luteinizing Hormone