[Pregnancy obtained by intracytoplasmic oocyte injection of spermatozoa retrieved from a cryopreserved testicular biopsy].
We have obtained a clinical pregnancy in a 26 year old patient by means of in vitro oocyte fertilization by intracytoplasmic injection of spermatozoa obtained from a cryopreserved testicular biopsy. In a first attempt performed with fresh biopsy material, the woman became pregnant, but the pregnancy ended in a spontaneous abortion. In a second cycle, the spermatozoa were retrieved from a cryopreserved sample saved from the first attempt. Twelve metaphase II oocytes were collected and injected; from these, nine became fertilized, three preembryos were transferred at the eighth-cell stage and the other six were cryopreserved. An ongoing clinical pregnancy was obtained with two gestational sacs.
Quintans
C J
CJ
Halitus Instituto Médico, Buenos Aires, Argentina.
Valzacchi
G R
GR
Donaldson
M J
MJ
Blanco
L A
LA
Pasqualini
R S
RS
D000328:Adult; D001706:Biopsy; D015925:Cryopreservation; D003593:Cytoplasm; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007267:Injections; D008297:Male; D009865:Oocytes; D013094:Spermatozoa; D013737:Testis / Q000166:cytology*