Potentiation of cytotoxicity against a CEA positive cell line by the bispecific antibody OH1 using LAK cells from cancer-bearing patients.
Bispecific antibody, OH1 was developed, which simultaneously recognizes CD3 on T cells and carcinoembryonic antigen (CEA) on tumor cells. Although there was not significant difference, PBMCs pretreated with OH1 showed tendency to prolong survival of KATO III-injected SCID mice compared with control groups. although cytotoxicity of LAK cells from cancer-bearing patients was suppressed compared with that of cells of healthy donors, LAK cells from the patients pretreated with OH1 showed significantly increased cytotoxicity against KATO III cells. These results suggest that OH1 could be useful in immunotherapy with LAK cells for carcinomas that express CEA on the membrane.
Second Department of Surgery, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan.
M
M
Baba
T
T
Hideshima
Y
Y
Yamashita
T
T
Shirakusa
D000328:Adult; D000368:Aged; D000818:Animals; D018033:Antibodies, Bispecific / Q000627:therapeutic use*; D017252:CD3 Complex / Q000032:analysis / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002470:Cell Survival; D003110:Colonic Neoplasms / Q000276:immunology / Q000473:pathology; D003602:Cytotoxicity, Immunologic; D005260:Female; D005706:Gallbladder Neoplasms / Q000276:immunology / Q000473:pathology; D006801:Humans; D007167:Immunotherapy; D015979:Killer Cells, Lymphokine-Activated; D008297:Male; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D009367:Neoplasm Staging; D012004:Rectal Neoplasms / Q000276:immunology / Q000473:pathology; D013274:Stomach Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D016019:Survival Analysis; D013997:Time Factors
D018033:Antibodies, Bispecific; D017252:CD3 Complex; D002272:Carcinoembryonic Antigen