Spectroscopic characterization of a binuclear metal site in Bacillus cereus beta-lactamase II.
The zinc metalloenzyme beta-lactamase II (betaLII) from Bacillus cereus has been overexpressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and the metal binding properties of recombinant betaLII toward Zn(II) and Co(II) have been studied by fluorescence and activity measurements. The apoenzyme is able to bind two metal ion equivalents, which confer on betaLII its maximum enzymatic efficiency. The enzyme is partially active with one metal ion equivalent. The diCo(II) and a mixed Zn(II)Co(II) derivative of betaLII were obtained and probed by electronic and paramagnetic NMR spectroscopy. In the high-affinity site, the metal is bound to three His residues and a solvent molecule, adopting a tetrahedral geometry. A Cys, a His, and an Asp residue are coordinated to the low-affinity metal site, together with two or three solvent molecules. This coordination polyhedron resembles the binuclear metal site of the Bacteroides fragilis beta-lactamase [Concha, N., Rasmussen, B. A., Bush, K., and Herzberg, O. (1996) Structure 4, 823-836; Carfi, A., Duée, E., Paul-Soto, R., Galleni, M., Frère, J. M., and Dideberg, O. (1998) Acta Crystallogr. D54, 47-57] but differs from that resulting from the X-ray study of betaLII [Carfi, A., Pares, S., Duée, E., Galleni, M., Duez, C., Frère, J. M., and Dideberg, O. (1995) EMBO J. 14, 4914-4921]. These results suggest that this binuclear metal site may be a general feature of metallo-beta-lactamases.
Orellano
E G
EG
Area Biofísica and PROMUBIE, Molecular Biology Division, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Argentina.
Girardini
J E
JE
Cricco
J A
JA
Ceccarelli
E A
EA
Vila
A J
AJ
D000595:Amino Acid Sequence; D001409:Bacillus cereus / Q000201:enzymology*; D001665:Binding Sites; D002510:Cephalosporinase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003035:Cobalt / Q000737:chemistry; D017124:Conserved Sequence; D004926:Escherichia coli / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D015032:Zinc / Q000737:chemistry*
D011994:Recombinant Proteins; D003035:Cobalt; D002510:Cephalosporinase; D015032:Zinc