Cytochemical localization of ouabain-sensitive, K+-dependent p-nitrophenylphosphatase activity in the choroid plexus of normal and reserpinized guinea pigs.
High doses of reserpine induce depletion of biogenic amines. The K-NPPase activity of choroid plexus was determined after one-shot reserpine administration using cerium-based cytochemistry. In normal untreated animals, reaction product was found on the microvilli of the choroidal epithelium but was almost undetectable 3 and 7 days after reserpinization. At 20 days after reserpinization, however, it was detectable. These findings suggested that reserpine decreased the choroidal Na,K-ATPase activity, and that catecholamines might be essential to maintain normal choroidal Na,K-ATPase activity. (J Histochem Cytochem 46: 975-976, 1998)
Department of Otorhinolaryngology, Eye Ear Nose and Throat Hospital, Shanghai Medical University, Shanghai, P.R. China.
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Dai
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Kanoh
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000818:Animals; D002831:Choroid Plexus / Q000378:metabolism* / Q000648:ultrastructure; D006168:Guinea Pigs; D008871:Microvilli / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000502:physiology*; D012110:Reserpine / Q000494:pharmacology*
D010042:Ouabain; D012110:Reserpine; D009597:4-Nitrophenylphosphatase; D011188:Potassium