Enzymatic preparation of radiolabeled linear maltodextrins and cyclodextrins of high specific activity from [14C] maltose using amylomaltase, cyclodextrin glucosyltransferase and cyclodextrinase.
Radiolabeled linear and cyclic maltodextrins of high specific radioactivity were prepared using enzymes involved in maltodextrin metabolism. 14C-Labeled maltose was the starting material yielding products of identical specific radioactivity with respect to glucosyl residues. The enzymatic steps involved: i) Formation of linear 14C-labeled maltodexrins (< maltooctaose) using amylomaltase from Escherischia coli; ii) Cyclisation to alpha-cyclodextrin using cyclodextrin-glucosyltransferase of Kiebsiella oxytoca M5a1; iii) Removal of the remaining linear dextrins by amyloglucosidase. The products were purified by paper chromatography, or maltohexaose was specifically obtained from purified alpha-cyclodextrin by the action of cyclodextrinase of K. oxytoca M5a1.
Lehrstuhl für Mikrobiologie der Universität München, Germany.
M
M
Pajatsch
A
A
Böck
W
W
Boos
D001345:Autoradiography; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005964:Glucosyltransferases / Q000378:metabolism*; D006004:Glycogen Debranching Enzyme System / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007553:Isotope Labeling; D007709:Klebsiella / Q000201:enzymology; D008320:Maltose / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
D002250:Carbon Radioisotopes; D003505:Cyclodextrins; D006004:Glycogen Debranching Enzyme System; D011134:Polysaccharides; D008320:Maltose; C008315:maltodextrin; D005964:Glucosyltransferases; C020569:4 alpha-glucanotransferase; D006026:Glycoside Hydrolases; C048745:cyclomaltodextrinase