Sexing of carcass remains of the Sika deer (Cervus nippon) using PCR amplification of the Sry gene.
In order to determine the sex of carcass remains of the Sika deer (Cervus nippon), we improved a polymerase chain reaction (PCR) technique for amplification of the Sika deer Sry, a male-specific DNA region on the mammalian Y chromosome. From the nucleotide sequence of the Sry region obtained here, PCR primers, MT1 and MT2, capable of amplifying a shorter Sry region were newly designed, and a microsatellite locus was used as a positive control. Using these primers, 96 of 109 sex-unknown fawns (88%, 96/109) were successfully sexed (46 males and 50 females) regardless of the conditions of carcasses found in the field. The results and the methodology could greatly contribute to the study of the mortality pattern of the Sika deer population.
Laboratory of Wildlife Biology, Graduate School of Veterinary Science, Hokkaido University, Sapporo, Japan.
M
M
Takahashi
R
R
Masuda
H
H
Uno
M
M
Yokoyama
M
M
Suzuki
M C
MC
Yoshida
N
N
Ohtaishi
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D003670:Deer; D005260:Female; D006801:Humans; D007564:Japan; D008297:Male; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012732:Sex Determination Analysis / Q000379:methods / Q000662:veterinary*; D019849:Sex Determination Processes; D051876:Sex-Determining Region Y Protein; D012756:Sheep; D014157:Transcription Factors / Q000235:genetics; D014998:Y Chromosome
D017931:DNA Primers; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors