Phosphate permeases of Saccharomyces cerevisiae.
The PHO84 and PHO89 genes of Saccharomyces cerevisiae encode two high-affinity phosphate cotransporters of the plasma membrane. Hydropathy analysis suggests a secondary structure arrangements of the proteins in 12 transmembrane domains. The derepressible Pho84 and Pho89 transporters appear to have characteristic similarities with the phosphate transporters of Neurospora crassa. The Pho84 protein catalyzes a proton-coupled phosphate transport at acidic pH, while the Pho89 protein catalyzes a sodium-dependent phosphate uptake at alkaline pH. The Pho84 transporter can be stably overproduced in the cytoplasmic membrane of Escherichia coli, purified and reconstituted in a functional state into proteoliposomes.
Department of Engineering and Natural Sciences, Växjö University, Sweden. Bengt.Persson@itn.hv.se
B L
BL
Persson
A
A
Berhe
U
U
Fristedt
P
P
Martinez
J
J
Pattison
J
J
Petersson
R
R
Weinander
D000595:Amino Acid Sequence; D000818:Animals; D001693:Biological Transport, Active; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010755:Organophosphates / Q000378:metabolism; D028061:Phosphate Transport Proteins; D017433:Protein Structure, Secondary; D028081:Proton-Phosphate Symporters; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters
D002352:Carrier Proteins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D010755:Organophosphates; C105495:PHO84 protein, S cerevisiae; C493567:PHO89 protein, S cerevisiae; D028061:Phosphate Transport Proteins; D028081:Proton-Phosphate Symporters; D029701:Saccharomyces cerevisiae Proteins; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters; C055831:phosphate permease