Quantitative analysis of thuringiensin by high-performance liquid chromatography using adenosine monophosphate as an internal standard.
An analytical method for thuringiensin using adenosine monophosphate (AMP) as an internal standard is established. AMP, with high stability and availability, is an appropriate internal standard for thuringiensin quantitative analysis using high-performance liquid chromatography and ultraviolet absorbance detection at 260 nm. A good correlation between the concentration of thuringiensin and the peak-area ratios of thuringiensin to AMP is demonstrated. From this general equation of linear regression line, the concentration of thuringiensin can be assessed in fermentation broth or semi-purified product.
Liu
C M
CM
Institute of Biotechnology, National Dong Hwa University, Shoufeng, Taiwan, Republic of China.
Tzeng
Y M
YM
D000241:Adenosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D000249:Adenosine Monophosphate; D001413:Bacillus thuringiensis / Q000378:metabolism; D001427:Bacterial Toxins; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D004355:Drug Stability; D005285:Fermentation; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013400:Sugar Acids / Q000032:analysis* / Q000737:chemistry
D001427:Bacterial Toxins; D013400:Sugar Acids; D000249:Adenosine Monophosphate; C068295:thuringiensin; D000241:Adenosine