Evaluation of product analogues for purification of penicillinase by affinity chromatography.
Among different matrices prepared, ampicilloic acid-polymer matrix offered 86.7% adsorption, 95% elution and 82.4% overall recovery of penicillinase. The structure of both the side chain and penicilloic or cephalosporoic acid moieties contribute to the affinity interactions.
Research and Development, Hindustan Antibiotics Ltd., Pimpri, Pune, India.
K K
KK
Kumar
B S
BS
Deshpande
S S
SS
Ambedkar
J G
JG
Shewale
D000327:Adsorption; D001409:Bacillus cereus / Q000201:enzymology; D002511:Cephalosporins / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods; D005285:Fermentation; D008024:Ligands; D010397:Penicillanic Acid / Q000031:analogs & derivatives / Q000737:chemistry; D010405:Penicillinase / Q000032:analysis / Q000302:isolation & purification*
D002511:Cephalosporins; D008024:Ligands; C008782:penicilloic acid; D010397:Penicillanic Acid; C030735:7-aminocephalosporanic acid; D010405:Penicillinase