Transformation of industrial dyes by manganese peroxidases from Bjerkandera adusta and Pleurotus eryngii in a manganese-independent reaction.
We investigated the transformation of six industrial azo and phthalocyanine dyes by ligninolytic peroxidases from Bjerkandera adusta and other white rot fungi. The dyes were not oxidized or were oxidized very little by Phanerochaete chrysosporium manganese peroxidase (MnP) or by a chemically generated Mn3+-lactate complex. Lignin peroxidase (LiP) from B. adusta also showed low activity with most of the dyes, but the specific activities increased 8- to 100-fold when veratryl alcohol was included in the reaction mixture, reaching levels of 3.9 to 9.6 U/mg. The B. adusta and Pleurotus eryngii MnP isoenzymes are unusual because of their ability to oxidize aromatic compounds like 2,6-dimethoxyphenol and veratryl alcohol in the absence of Mn2+. These MnP isoenzymes also decolorized the azo dyes and the phthalocyanine complexes in an Mn2+-independent manner. The reactions with the dyes were characterized by apparent Km values ranging from 4 to 16 microM and specific activities ranging from 3.2 to 10.9 U/mg. Dye oxidation by these peroxidases was not increased by adding veratryl alcohol as it was in LiP reactions. Moreover, the reaction was inhibited by the presence of Mn2+, which in the case of Reactive Black 5, an azo dye which is not oxidized by the Mn3+-lactate complex, was found to act as a noncompetitive inhibitor of dye oxidation by B. adusta MnP1.
Heinfling
A
A
FG Microbial Ecology, Technical University of Berlin, D-10587 Berlin, Germany. hein0654@mailszrz.zrz.tu-berlin.de
Martínez
M J
MJ
Martínez
A T
AT
Bergbauer
M
M
Szewzyk
U
U
D001391:Azo Compounds / Q000378:metabolism; D001487:Basidiomycota / Q000201:enzymology* / Q000254:growth & development; D001673:Biodegradation, Environmental; D004396:Coloring Agents / Q000378:metabolism*; D007211:Indoles / Q000378:metabolism; D054833:Isoindoles; D007700:Kinetics; D008031:Lignin / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*
D001391:Azo Compounds; D004396:Coloring Agents; D007211:Indoles; D054833:Isoindoles; D008345:Manganese; D008031:Lignin; D010544:Peroxidases; C051129:manganese peroxidase; C013647:phthalocyanine