Antisense in abundance: the ribosome as a vehicle for antisense RNA.
Insertions at some sites within rRNA variable regions can be tolerated without affecting rRNA function. Antisense RNAs inserted at such sites in the T. thermophila rRNA can eliminate phenotypically or immunologically detectable gene expression of three genes tested. This unusually effective antisense activity is probably due to the abundance, stability and favourable intracellular localization of these antisense rRNAs with respect to mRNAs. Since antisense RNAs function very well as a part of the rRNA, rRNA might also be useful as a vehicle for other RNAs that might affect cell function such as protein binding sites or trans-acting ribozymes. The robust function of the antisense ribosome system in T. thermophila should allow the use of this system to specifically suppress gene expression and to clone genes by their null or hypomorphic phenotypes. The use of the antisense ribosome in other eukaryotes has yet to be explored, but the realization of this goal is well within the realm of possibility.
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
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D000818:Animals; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D016372:RNA, Antisense; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000235:genetics; D012270:Ribosomes / Q000378:metabolism*; D016808:Tetrahymena thermophila / Q000235:genetics / Q000378:metabolism
D016372:RNA, Antisense; D012333:RNA, Messenger; D012335:RNA, Ribosomal