Tyrosine-rich crystals associated with oncocytic salivary gland neoplasms.
OBJECTIVE
Crystalloids have been identified ultrastructurally within the epithelial cells of Warthin's tumors, but there have been no studies characterizing crystals or crystalloids in Warthin's tumors by light microscopy. The finding of abundant needle-shaped crystals in a fine-needle aspirate of a cystadenoma of the parotid prompted us to examine the prevalence of crystals and crystalloids in oncocytic salivary gland neoplasms.


METHODS
Ninety-seven oncocytic neoplasms (93 Warthin's tumors, 3 cystadenomas, and 1 oncocytoma) excised at our institution between 1950 and 1996 were examined, to identify crystals. Neoplasms with crystals were further characterized by means of a variety of histochemical stains and electron microscopy. Ninety-nine pleomorphic adenomas were similarly reviewed.


RESULTS
Seven cases with crystals were identified. Five of these were Warthin's tumors, 1 was a cystadenoma, and 1 was an oncocytoma. The crystals were noted within tumor cysts but were not limited to the neoplasms. The crystals were predominantly either needle-shaped or tabular, but some cases contained mixtures of both as well as intermediate forms. They stained pink with hematoxylin-eosin, although the tabular forms also exhibited a focal yellow hue. The crystals were not discernible under polarized light. They stained a red-brown color with Millon's reagent, which indicated the presence of tyrosine. Trichrome, periodic acid-Schiff stain with diastase, alcian blue (pH 2.5), and Congo red stains were negative. Electron microscopy revealed sharply defined, elongate, electron-dense structures with periodicity, both extracellular and within epithelial cells. No crystals or crystalloids were identified in any of 99 pleomorphic adenomas reviewed.


CONCLUSIONS
The findings indicate that tyrosine-rich crystals associated with several oncocytic salivary gland neoplasms are morphologically, histochemically, and ultrastructurally distinct from previously described tyrosine-rich crystalloids and collagenous crystalloids of pleomorphic adenomas. Although the crystals appear to form by the assembly of small units within epithelial cells, the exact mode of formation remains unclear.
Department of Laboratory Medicine and Pathology, Veterans Affairs Medical Center, and the University of Minnesota School of Medicine, Minneapolis, USA.
M Z
MZ
Gilcrease
F S
FS
Nelson
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M
Guzman-Paz
D000235:Adenolymphoma / Q000737:chemistry* / Q000473:pathology; D018249:Adenoma, Oxyphilic / Q000737:chemistry / Q000473:pathology; D001707:Biopsy, Needle; D004396:Coloring Agents; D003460:Crystallization; D003537:Cystadenoma / Q000737:chemistry / Q000473:pathology; D006801:Humans; D008854:Microscopy, Electron; D012468:Salivary Gland Neoplasms / Q000737:chemistry* / Q000473:pathology; D014443:Tyrosine / Q000032:analysis*
D004396:Coloring Agents; D014443:Tyrosine