Characterization of a new human embryonal rhabdomyosarcoma cell line, RMS-GR.
A human tumor cell line designated RMS-GR was established from an embryonal rhabdomyosarcoma. The monolayer cells were polygonal, round or spindle-shaped. The RMS-GR cell line became stable with a doubling time of 42 h. Tumorigenicity of the cells was confirmed by heterotransplantion into nude mice. Electron microscopic images showed typical cytoplasmic inclusion of aggregated intermediate filaments and myofibril-like thin filaments. The expression of desmin, vimentin, actin and human myoglobin was recognized by cytofluorometric analyses, and a large fraction of CK-MM and small fractions of CK-BB and MCK-1 isoenzymes were found. Chromosomal analysis showed that the modal chromosome number was consistently near triploid with structural abnormalities mostly involving chromosomes 1, 3 and 8, and additional unidentified markers. No alteration of chromosome 2 was observed. The RMS-GR cell line may provide a system to identify genes which are involved in the pathogenic mechanism of rhabdomyosarcomas, and to investigate the modulation of myogenic differentiation.
Fernández
J E
JE
Department of Morphological Sciences, School of Medicine, University of Granada, Spain.
Prados
J
J
Melquizo
C
C
Arena
N
N
Malavasi
F
F
Alvarez
L
L
Aránega
A
A
D000199:Actins / Q000032:analysis; D000818:Animals; D002455:Cell Division; D002869:Chromosome Aberrations; D003402:Creatine Kinase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes; D007633:Keratins / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D009211:Myoglobin / Q000032:analysis; D009368:Neoplasm Transplantation; D018233:Rhabdomyosarcoma, Embryonal / Q000737:chemistry / Q000235:genetics / Q000473:pathology*; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D014746:Vimentin / Q000032:analysis
D000199:Actins; D007527:Isoenzymes; D009211:Myoglobin; D014746:Vimentin; D007633:Keratins; D003402:Creatine Kinase