mafA, a novel member of the maf proto-oncogene family, displays developmental regulation and mitogenic capacity in avian neuroretina cells.
Transcription factors of the Maf proto-oncogene family have been shown to participate in the regulation of several differentiation specific genes. We previously reported that a member(s) of this family is involved in the regulation of the neuroretina specific gene, QR1, through a promoter region, designated the A box, that is closely related to the Maf recognition element (MARE). We undertook an identification of Maf family genes expressed in the quail neuroretina (QNR) and we report the isolation of mafA, a gene encoding a novel member of the large Maf proteins subgroup. Expression of this gene is developmentally regulated in the neuroretina. MafA is able to bind to MARE sequence and to heterodimerize with v-Maf, MafB, Jun and Fos, but not with the small MafF and MafK proteins. Accordingly, it is able to transactivate the QR1 promoter A box. We also show that increased expression of mafA induces sustained proliferation of postmitotic QNR cells.
Unité mixte de recherche 146 du Centre National de la Recherche Scientifique, Institut Curie, Orsay, France.
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Benkhelifa
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Provot
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Lecoq
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Pouponnot
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Calothy
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Felder-Schmittbuhl
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics; D005786:Gene Expression Regulation; D008934:Mitogens / Q000235:genetics; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology*; D051256:Oncogene Protein v-maf; D015513:Oncogene Proteins / Q000378:metabolism; D009856:Oncogene Proteins, Viral / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D011784:Quail / Q000235:genetics*; D012160:Retina / Q000166:cytology*; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors; D015533:Transcriptional Activation; D014764:Viral Proteins
D030161:Avian Proteins; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D008934:Mitogens; D051256:Oncogene Protein v-maf; D015513:Oncogene Proteins; D009856:Oncogene Proteins, Viral; D011518:Proto-Oncogene Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; C091516:QR1 protein, Coturnix japonica; D015534:Trans-Activators; D014157:Transcription Factors; D014764:Viral Proteins