Induction of entry into the stationary growth phase in Pseudomonas aeruginosa by N-acylhomoserine lactone.
N-acylhomoserine lactone (AHSL, autoinducer) is capable of regulating a set of genes by sensing cell density and developing an intercellular communication in Pseudomonas aeruginosa. Addition of AHSL in the exponential growth phase, regardless of cell density, induces a repression of cell growth of P. aeruginosa, an expression of stationary phase specific factor sigma 8 in vivo and a morphological change into smaller spherical shape indistinguishable from that in the stationary phase. It is demonstrated that AHSL can trigger an entry of bacteria into stationary phase as a growth controlling signal.
Department of Bacteriology, Yokohama City University School of Medicine, Japan.
Z
Z
You
J
J
Fukushima
K
K
Tanaka
S
S
Kawamoto
K
K
Okuda
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000187:drug effects* / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006714:Homoserine / Q000031:analogs & derivatives* / Q000494:pharmacology; D015151:Immunoblotting; D018625:Microscopy, Atomic Force; D016133:Polymerase Chain Reaction; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D012329:RNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification; D012808:Sigma Factor / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D001426:Bacterial Proteins; C092312:N-butyrylhomoserine lactone; D012329:RNA, Bacterial; D012333:RNA, Messenger; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D006714:Homoserine; D015107:4-Butyrolactone