Inhibitory effect of Cowdria ruminantium on the expression of MHC class I and class II molecules on bovine endothelial cells.
Bovine endothelial cells constitutively express MHC class I molecules, whereas MHC class II molecules can be induced by interferon gamma (IFN gamma) treatment. Endothelial cells may play a role as antigen presenting cells (APC), but their role in the immune response against Cowdria ruminantium is unknown. We have studied the effect of C. ruminantium infection on the expression of MHC class I and class II molecules on the surface of bovine endothelial cells. Bovine brain endothelial cells (BBEC) from the microvasculature and bovine umbilical endothelial cells (BUEC) from the macrovasculature were cultured in vitro, infected with different concentration of Cowdria, and treated or not with IFN gamma. We observed that Cowdria induced a reduction of MHC molecules expression on the surface of BUEC and BBEC. This inhibitory effect of Cowdria on MHC molecules could affect the capacity of endothelial cells to act as efficient antigen-presenting cells.
CIRAD-EMVT, BP 515, 97165 Point à Pitre, Guadeloupe.
N
N
Vachiéry
I
I
Trap
P
P
Totté
D
D
Martinez
A
A
Bensaid
D000818:Animals; D001921:Brain / Q000098:blood supply; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D017077:Culture Media, Conditioned; D016995:Ehrlichia ruminantium / Q000276:immunology*; D004730:Endothelium, Vascular / Q000276:immunology* / Q000382:microbiology*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis*; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis*; D008833:Microcirculation; D014469:Umbilical Arteries
D017077:Culture Media, Conditioned; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II