Similar IL-5, IL-3, and GM-CSF syntheses by eosinophils in the jejunal mucosa of patients with celiac disease and dermatitis herpetiformis.
Celiac disease (CD) and dermatitis herpetiformis (DH) are gluten-sensitive diseases with different clinical features that can initiate similar intestinal changes. The flat-destructive stage corresponds to severe lesions involving activated T-cells. However, other inflammatory cells such as eosinophils are also abundant. The mechanisms for the intestinal recruitment of eosinophils in patients with CD and DH remain unknown. Eosinophil recruitment and activation are induced in vitro by three main cytokines: interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, IL-3, IL-5, and GM-CSF were detected by immunohistochemistry in all patients with CD and DH but not in the control group. By ultrastructural immunogold staining, these three cytokines had the same subcellular localization in the granule matrix of eosinophils. This result suggests that eosinophils may be involved in the immune response at the flat-destructive stage of both CD and DH.
Laboratoire de Recherche sur les Maladies Inflammatoires Intestinales, Centre Hospitalier Universitaire (CHU), Lille, France.
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P
Desreumaux
E
E
Delaporte
J F
JF
Colombel
M
M
Capron
A
A
Cortot
A
A
Janin
D000293:Adolescent; D000328:Adult; D002446:Celiac Disease / Q000276:immunology* / Q000473:pathology; D003594:Cytoplasmic Granules / Q000276:immunology; D003874:Dermatitis Herpetiformis / Q000276:immunology* / Q000473:pathology; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis*; D006801:Humans; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007377:Interleukin-3 / Q000096:biosynthesis*; D015848:Interleukin-5 / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology; D007583:Jejunum / Q000276:immunology / Q000473:pathology; D008297:Male; D016253:Microscopy, Immunoelectron; D008875:Middle Aged
D007377:Interleukin-3; D015848:Interleukin-5; D016178:Granulocyte-Macrophage Colony-Stimulating Factor