Essential Cys-Pro-Cys motif of Caenorhabditis elegans copper transport ATPase.
Caenorhabditis elegans putative copper ATPase (CUA-1) had been functionally expressed in a yeast delta ccc2 mutant (copper ATPase gene disruptant). We found that CUA-1 with Cys-Pro-Cys to Cys-Pro-Ala mutation could not rescue the yeast delta ccc2 mutant, suggesting that the carboxyl terminal cysteine residue in the conserved Cys-Pro-Cys motif is essential for copper transport.
Division of Biological Sciences, Osaka University, Japan.
T
T
Yoshimizu
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Omote
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Wakabayashi
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Sambongi
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Futai
D000251:Adenosine Triphosphatases / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D017173:Caenorhabditis elegans / Q000201:enzymology*; D003300:Copper / Q000493:pharmacokinetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009842:Oligopeptides / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D009842:Oligopeptides; D003300:Copper; D000251:Adenosine Triphosphatases