Evaluation of mannitol salt agar for detection of oxacillin resistance in Staphylococcus aureus by disk diffusion and agar screening.
Mannitol salt agar was evaluated for detection of oxacillin resistance in 136 Staphylococcus aureus isolates. All mecA-positive isolates (n = 54) were correctly categorized as oxacillin resistant by the disk diffusion test (1-microgram disk; zone diameter, < 16 mm); the specificity was 97.6%. Agar screening (2 micrograms of oxacillin per ml) revealed a sensitivity of 98.1% and a specificity of 95.1%.
Institut für Hygiene, Freie Universität Berlin, Germany.
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D003470:Culture Media; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D006801:Humans; D008353:Mannitol / Q000494:pharmacology*; D016106:Methicillin Resistance / Q000235:genetics; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010068:Oxacillin / Q000494:pharmacology*; D010406:Penicillins / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012372:ROC Curve; D012680:Sensitivity and Specificity; D012965:Sodium Chloride; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
D003470:Culture Media; D010406:Penicillins; D008353:Mannitol; D012965:Sodium Chloride; D010068:Oxacillin