Distribution of G serotypes and P genotypes of bovine group A rotavirus isolated in Japan.
OBJECTIVE
To identify the distribution of G serotype and P genotype of bovine group A rotavirus in Japan.


METHODS
Detection, isolation, electropherotyping, G serotyping and P genotyping of bovine group A rotavirus in 167 diarrhoeal faecal samples.


METHODS
Bovine group A rotavirus was detected and isolated, and bovine group A rotavirus isolates were identified electropherotype by polyacrylamide gel electrophoresis and G serotype and P genotype by reverse transcription-poly-merase chain reaction.


RESULTS
Twenty-eight bovine group A rotavirus strains were isolated from 167 samples. Electropherotypes of the bovine group A rotavirus isolates were identified as long-genome electropherotype and the most prevalent combination of G serotype and P genotype of the bovine group A rotavirus isolates was G6P5 (25/28; 89.3%), followed by G6P11 (2/28; 7.1%) and G10P11 (1/28; 3.6%).


CONCLUSIONS
These results suggest that the bovine group A rotavirus exhibiting G6P5 is the most common in Japan.
Fukai
K
K
Department of Preventive Veterinary, Medicine and Animal Health, Nihon University School of Veterinary Medicine, Kanagawa, Japan.
Sakai
T
T
Kamata
H
H
D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D002460:Cell Line; D017931:DNA Primers / Q000737:chemistry; D003967:Diarrhea / Q000662:veterinary* / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology*; D005838:Genotype; D007564:Japan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012330:RNA, Double-Stranded / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000662:veterinary* / Q000821:virology; D012703:Serotyping / Q000662:veterinary
D017931:DNA Primers; D012330:RNA, Double-Stranded; D012367:RNA, Viral