The use of lectin-dependent lysis for detection of cytotoxic T lymphocytes in outbred animals.
The assessment of cytotoxic T lymphocyte (CTL) specificity and levels of activity is often important in the analysis of an immune response to viral infection or vaccination. However, the restriction of CTL lysis to cells bearing self major histocompatibility complex proteins makes this analysis difficult in outbred animals. The data presented here demonstrate that lysis by CTL can be measured on allogeneic or xenogeneic target cells in the presence of Concanavalin A, if the CTL are first restimulated in vitro with specific antigen. Using this method, CTL from a large number of animals could be conveniently assayed on a single target cell line.
CSIRO Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria. marion@aahl.dah.csiro.au
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Andrew
D000818:Animals; D001820:Bluetongue virus / Q000276:immunology; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D037102:Lectins / Q000276:immunology; D012756:Sheep; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D037102:Lectins; D003208:Concanavalin A