Increased tumor establishment and growth after open vs laparoscopic bowel resection in mice.
BACKGROUND
Surgery can suppress immune function and facilitate tumor growth. Several studies have demonstrated better preservation of immune function following laparoscopic procedures. Our laboratory has also shown that tumors are more easily established and grow larger after sham laparotomy than after pneumoperitoneum in mice. The purpose of this study was to determine if the previously reported differences in tumor establishment and growth would persist in the setting of an intraabdominal manipulation.


METHODS
Syngeneic mice received intradermal injections of tumor cells and underwent either an open or laparoscopic cecal resection. In study 1, the incidence of tumor development was observed after a low dose inoculum; whereas in study 2, tumor mass was compared on postoperative day 12 after a high-dose inoculum.


RESULTS
In study 1, tumors were established in 5% of control mice, 30% of laparoscopy mice, and 83% of open surgery mice (p < 0.01 for all comparisons). In study 2, open surgery group tumors were 1.5 times as large as laparoscopy group tumors (p < 0.01), which were 1.5 times as large as control group tumors (p < 0.02).


CONCLUSIONS
We conclude that tumors are more easily established and grow larger after open laparoscopic bowel resection in mice.
Department of Surgery, Columbia University College of Physicians and Surgeons, New York, NY, USA.
J D
JD
Allendorf
M
M
Bessler
K D
KD
Horvath
M R
MR
Marvin
D A
DA
Laird
R L
RL
Whelan
D000818:Animals; D002431:Cecostomy / Q000379:methods; D016923:Cell Death; D002455:Cell Division / Q000502:physiology; D016009:Chi-Square Distribution; D005260:Female; D007108:Immune Tolerance / Q000502:physiology; D010535:Laparoscopy / Q000009:adverse effects* / Q000401:mortality; D008325:Mammary Neoplasms, Experimental / Q000401:mortality / Q000601:surgery*; D051379:Mice; D008809:Mice, Inbred C3H; D009364:Neoplasm Recurrence, Local / Q000209:etiology* / Q000473:pathology; D012016:Reference Values; D013514:Surgical Procedures, Operative / Q000009:adverse effects* / Q000401:mortality; D015996:Survival Rate; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000276:immunology