Incorporation of dispersion into the theory of electrophoresis of DNA and its complexes.
Incorporation of the dispersion coefficient into the theory of the mobility-shift assay for DNA-protein complexes was highly successful largely due to increased mathematical rigor. A model simulating electrophoretic migration of DNA across the phase boundary between the initial zone of macromolecule and the gel lane predicts the peak asymmetry observed experimentally. It also predicts that, under the agency of the dispersion coefficient, the peak will become progressively more symmetrical during migration along the gel lane.
Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver 80262, USA.
J R
JR
Cann
D004247:DNA / Q000032:analysis*; D004586:Electrophoresis; D008432:Mathematical Computing
D004247:DNA