Carbohydrates engineered at antibody constant domains can be used for site-specific conjugation of drugs and chelates.
To improve the efficiency of site-specific conjugation of chelates and drugs to antibodies, and to minimize the incidence of immunoreactivity perturbation to the resultant immunoconjugates, Asn-linked oligosaccharide moieties were designed and engineered into the constant domains of a humanized anti-CD22 monoclonal antibody, hLL2. From 10 potential glycosylation mutants, two CH1 domain glycosylation sites, HCN1 and HCN5, were identified that were positioned favorably for glycosylation. The carbohydrate (CHO) chains attached at these sites were differentially processed so that HCN5-CHOs were physically larger than HCN1-CHOs. Although both the CH1-appended CHOs, and the LL2 Vkappa-appended CHOs conjugated efficiently with small chelates, the HCN5-CHOs, due to the structural and positional superiority, appear to be a better conjugation site for large drug complexes, such as 18 kDa doxorubicin (DOX)-dextran.
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Z
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Immunomedics, Morris Plains, NJ 07950, USA.
Sharkey
R M
RM
Hansen
H J
HJ
Shih
L B
LB
Govindan
S V
SV
Shen
J
J
Goldenberg
D M
DM
Leung
S O
SO
D000595:Amino Acid Sequence; D000903:Antibiotics, Antineoplastic / Q000737:chemistry*; D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000235:genetics; D015703:Antigens, CD / Q000276:immunology; D000944:Antigens, Differentiation, B-Lymphocyte / Q000276:immunology; D001483:Base Sequence; D001665:Binding Sites; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry*; D015815:Cell Adhesion Molecules; D002614:Chelating Agents / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D004317:Doxorubicin / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D018796:Immunoconjugates / Q000737:chemistry*; D037102:Lectins; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009844:Oligosaccharides / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D051918:Sialic Acid Binding Ig-like Lectin 2; D013379:Substrate Specificity
D000903:Antibiotics, Antineoplastic; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C497867:CD22 protein, human; D002241:Carbohydrates; D015815:Cell Adhesion Molecules; D002614:Chelating Agents; D003911:Dextrans; D018796:Immunoconjugates; D037102:Lectins; D009844:Oligosaccharides; D010455:Peptides; D051918:Sialic Acid Binding Ig-like Lectin 2; D004317:Doxorubicin