Epidemiological markers in Neisseria meningitidis: an estimate of the performance of genotyping vs phenotyping.
In order to estimate the performance of genotypic vs phenotypic characterization of Neisseria meningitidis, 2 methods, DNA fingerprinting and multilocus enzyme electrophoresis (MEE), were assessed as regards applicability, reproducibility and discriminating capacity. 50 serogroup B and 52 serogroup C Neisseria meningitidis strains from 96 patients with meningococcal disease and 22 serogroup C strains from healthy carriers were investigated. Both methods were 100% applicable to meningococcal strains and results of DNA fingerprinting as well as of MEE were reproducible. The number of types defined by DNA fingerprinting and MEE as compared to that defined by phenotypic characteristics (serogroup, serotype, serosubtype and sulphonamide resistance) was as follows: for serogroup B strains from patients, 11 and 12 vs 8; for serogroup C strains from patients, 10 and 15 vs 8; and for serogroup C carrier strains, 12 and 19 genotypes vs 10 phenotypes were defined. By use of both DNA fingerprinting and MEE the number of genotypes defined for the 3 groups of strains was 14, 17 and 19, respectively. DNA fingerprinting and MEE showed a discriminating capacity superior to that of phenotyping, and as applied in the study MEE was superior to DNA fingerprinting. Clusters of invasive strains were reliably identified by phenotyping alone, whereas determination of identity of carrier strains and an invasive strain required genotyping.
Weis
N
N
Neisseria Department, Statens Serum Institut, Copenhagen, Denmark.
Lind
I
I
D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods; D005838:Genotype; D006801:Humans; D008589:Meningococcal Infections / Q000453:epidemiology; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics*; D010641:Phenotype; D015203:Reproducibility of Results; D012703:Serotyping
D004269:DNA, Bacterial