Expression of adult T cell leukemia-derived factor in human brain and peripheral nerve tissues.
In both nonneurological and Alzheimer brains, we examined the localization of adult T cell leukemia-derived factor (ADF) by immunohistochemistry, that of its mRNA by in situ hybridization, and its semiquantitative mRNA analyses by RT-PCR. Anti-ADF antibody gave positive staining of white matter astrocytes and Schwann cells in the posterior root. Their intense and abundant staining was seen in Alzheimer brains. In situ hybridization for ADF mRNA showed identical signals in the white matter astrocytes. The evidence was also confirmed by RT-PCR analysis. These results suggest that redox regulation may play a role in Alzheimer pathology.
Asahina
M
M
Department of Neurology, Chiba University, Japan.
Yamada
T
T
Yoshiyama
Y
Y
Yodoi
J
J
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism* / Q000473:pathology; D001253:Astrocytes / Q000378:metabolism; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009434:Neural Pathways / Q000378:metabolism; D010296:Parietal Lobe / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012583:Schwann Cells / Q000378:metabolism; D013126:Spinal Nerve Roots / Q000378:metabolism; D013879:Thioredoxins / Q000096:biosynthesis* / Q000235:genetics
D016207:Cytokines; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C055533:adult T cell leukemia-derived factor; D013879:Thioredoxins