Effect of Sulfo Lewis C on smoke inhalation injury in an ovine model.
OBJECTIVE
To evaluate the effect of Sulfo Lewis C (SO3-3âGal1-3GlcNAc-O(CH2)8-COOMe), a putative ligand of selectins, on smoke inhalation injury.


METHODS
Prospective animal study with concurrent controls.


METHODS
An animal laboratory.


METHODS
Twelve 1-yr-old female sheep, weighing 24 to 33 kg.


METHODS
Twelve sheep received nine exposure units of smoke generated by thermolysis of pine woodchips (80 g). Group 1 (n = 6) was untreated. Group 2 (n = 6) was treated with an intravenous infusion of Sulfo Lewis C after smoke exposure. Animals were killed 48 hrs after injury.


RESULTS
Cardiopulmonary variables and blood gases were measured serially. Granulocyte free-radical production was measured before smoke exposure and at 4 and 48 hrs after injury. Ventilation/perfusion distribution (VA/Q) was analyzed using the multiple inert gas elimination technique. Granulocyte free-radical production was increased after smoke exposure in both groups. Oxygenation was significantly improved by the administration of Sulfo Lewis C. VA/Q analysis demonstrated significantly less blood flow to low VA/Q lung segments in treated animals.


CONCLUSIONS
Selectin blockade attenuated lung injury after smoke exposure. These data support the hypothesis that neutrophils play a pivotal role in smoke inhalation injury.
Tasaki
O
O
U.S. Army Institute of Surgical Research, San Antonio, TX 78234-6315, USA.
Mozingo
D W
DW
Ishihara
S
S
Brinkley
W W
WW
Johnson
A A
AA
Smith
R H
RH
Srivastava
O
O
Mason
A D
AD
Pruitt
B A
BA
Cioffi
W G
WG
D000818:Animals; D005260:Female; D007983:Lewis Blood Group Antigens; D008833:Microcirculation; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009844:Oligosaccharides / Q000627:therapeutic use*; D011446:Prospective Studies; D012131:Respiratory Insufficiency / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D019087:Selectins / Q000187:drug effects* / Q000378:metabolism; D012756:Sheep; D015208:Smoke Inhalation Injury / Q000097:blood / Q000150:complications*; D013997:Time Factors
D007983:Lewis Blood Group Antigens; D009844:Oligosaccharides; D019087:Selectins; C113506:sulfo Lewis C