Interaction of per 3,6-anhydro-alpha cyclodextrins (alpha 36CD) and lead-alpha 36CD complex with biological systems.
The interactions of per (3,6 anhydro) alpha cyclodextrin (alpha 36CD) and of lead-alpha 36CD complex with biological systems were tested by NMR, ESR and electronic microscopy using erythrocytes and model membranes. It was found that the haemolytic activity of alpha 36CD alone was seven fold lower than that of natural alpha cyclodextrin (evaluated by the concentration inducing 50% haemolysis, DH50 = 35 mM). Conversely, the formation of the complex resulted in an increase of haemolytic properties, with DH50 of 1 mM. The mechanism proposed was an increased membrane diffusion by endocytosis of the complex, leading to higher amounts of intracellular lead.
Debouzy
J C
JC
CRSSA, Laboratoire RMN, Tronche, France.
Fauvelle
F
F
Gadelle
A
A
Baudin
C
C
Richard
M
M
Perly
B
B
Chouteau
F
F
Joets
J
J
Tazz
J J
JJ
Daveloose
D
D
D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D003505:Cyclodextrins / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000187:drug effects / Q000648:ultrastructure; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007854:Lead / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D009682:Magnetic Resonance Spectroscopy; D008823:Micelles; D008854:Microscopy, Electron; D010743:Phospholipids
D002614:Chelating Agents; D003505:Cyclodextrins; D008823:Micelles; D010743:Phospholipids; D007854:Lead