Detection of dopamine-tissue adducts.
Incubation of tritium labeled dopamine (3HDA) with a preparation of rat heart or bovine serum albumin in vitro, or the intravenous injection of 3HDA to rats, gives acid insoluble, tritium labeled material that is slowly converted to soluble labeled material (SLM) by heating in dilute perchloric acid. Determination of SLM may be useful in investigations of DA-tissue adducts formed when the catecholamine is delivered by intravenous infusion.
Department of Pharmacology, School of Medicine, University of Missouri, Columbia 65212, USA.
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Byington
D000818:Animals; D004298:Dopamine / Q000378:metabolism* / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D006358:Hot Temperature; D007275:Injections, Intravenous; D008297:Male; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017207:Rats, Sprague-Dawley; D014316:Tritium / Q000378:metabolism
D014316:Tritium; D004298:Dopamine