Prevention from hypoxia-induced apoptosis by pre-conditioning: a mechanistic approach in cultured neurons from fetal rat forebrain.
Molecular effects of pre-conditioning by 1-h hypoxia were investigated in cultured neurons from fetal rat forebrain, submitted the following day to a 6-h hypoxia that induces apoptosis. While preventing from apoptosis, pre-conditioning led to increased number of living neurons, DNA synthesis, with persistent overexpression of Bcl-2 and proliferating cell nuclear antigen (PCNA). Adaptative mechanisms would involve anti-apoptotic proteins and regulators of the cell cycle, to finally promote neuronal proliferation.
JE 2164-Adaptation Néonatale and Développement, Université Henri Poincaré, 24-30 rue Lionnois, B.P. 3069, 54013 Nancy Cedex, France.
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Bossenmeyer-Pourié
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JL
Daval
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D002453:Cell Cycle; D002455:Cell Division; D015687:Cell Hypoxia; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004247:DNA / Q000096:biosynthesis; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005333:Fetus; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D008938:Mitosis; D009336:Necrosis; D009474:Neurons / Q000166:cytology* / Q000502:physiology*; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D016548:Prosencephalon / Q000166:cytology* / Q000502:physiology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D013997:Time Factors
D016895:Culture Media, Serum-Free; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D004247:DNA