A galactose-specific lectin from the red marine alga Ptilota filicina.
A lectin from the red marine alga Ptilota filicina (PFL) was isolated by affinity chromatography on cross-linked guar gum. PFL agglutinated native and papain-treated human erythrocytes with preference for type O erythrocytes. The lectin was inhibited by galactose and its derivatives. The most potent inhibitors were p-Nitrophenyl-N-acetyl-alpha- and beta-D-galactosaminide. Porcine stomach mucin, bovine submaxillary gland mucin and asialo bovine mucin were also inhibitory. The M(r) of PFL, determined by gel filtration, was 56,900. SDS-PAGE gave one band with a subunit M(r) of 19,320, indicating the native protein to be a trimer of apparently identical subunits. PFL was shown to be rich in acidic and hydroxyl amino acids but low in basic amino acids. The ten N-terminal amino acids were Asx-Thr-Lys-Thr-Leu-Leu-Ala-.
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Brasil.
A H
AH
Sampaio
D J
DJ
Rogers
C J
CJ
Barwell
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D005690:Galactose; D005753:Gastric Mucosa / Q000737:chemistry; D006386:Hemagglutination Tests; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification*; D009077:Mucins / Q000737:chemistry*; D000461:Rhodophyta / Q000737:chemistry*; D012623:Seawater; D013363:Submandibular Gland / Q000737:chemistry; D013552:Swine
D000596:Amino Acids; D037102:Lectins; D009077:Mucins; D005690:Galactose