New assay technologies for high-throughput screening.
The use of high-throughput screening for early stage drug discovery imposes several constraints on the format of assays for therapeutic targets of interest. Homogeneous cell-free assays based on energy transfer, fluorescence polarization spectroscopy or fluorescence correlation spectroscopy provide the sensitivity, ease, speed and resistance to interference from test compounds needed to function in a high-throughput screening mode. Similarly, novel cell-based assays are now being adapted for high-throughput screening, providing for in situ analysis of a variety of biological targets. Finally, recent advances in assay miniaturization mark a transition to ultra high-throughput screening, ensuring that identification of lead compounds will not be the rate-limiting step in finding new drugs.
Silverman
L
L
Cadus Pharmaceutical Corporation, Tarrytown, NY 10591-6705, USA.
Campbell
R
R
Broach
J R
JR
D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D002625:Chemistry, Organic / Q000379:methods*; D015195:Drug Design; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004735:Energy Transfer; D017930:Genes, Reporter; D008322:Mammals; D008904:Miniaturization / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection / Q000379:methods