A 33 bp minisatellite repeat upstream of the 'mutated in colon cancer' gene at chromosome 5q21.
We have found a 33 bp minisatellite repeat in the 5'-flanking region of the mutated in colon cancer (MCC) gene at chromosome 5q21. Southern blot experiments demonstrated the locus specificity of the repeat. The number of repeat units varied between 5 and 11 with a heterozygosity of 0.56. The sequence 5'-AGG AGT GTG AAT GGG GCA TAG TGA ATG AGG GGA-3' of the repeat units does not match the consensus sequence of chi-related minisatellites. The minisatellite is not expressed as part of a gene transcription unit. However, it can be used as a tool for the detection of allelic changes at chromosome 5q21 on standard agarose gels.
Bugert
P
P
Dept. of Urology, Ruprecht-Karls-University of Heidelberg, Germany. peter.bugert@urz.uni-heidelberg.de
Kenck
C
C
Kovacs
G
G
D001483:Base Sequence; D002874:Chromosome Mapping; D002895:Chromosomes, Human, Pair 5; D003110:Colonic Neoplasms / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D011506:Proteins / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D011506:Proteins; D025521:Tumor Suppressor Proteins; C067857:MCC protein, human