The antioxidant action of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyra z in-3-one (MCLA), a chemiluminescence probe to detect superoxide anions.
The antioxidant effect of 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-alpha]pyraz in-3-one (MCLA), a Cypridina luciferin analog that acts as a chemiluminescence probe to detect O2.-, was investigated. MCLA produced a lag in oxygen consumption induced by cumene hydroperoxide in microsomes or by 2,2'-azobis (2-amidinopropane) dihydrochloride in liposomes and disappeared during the duration of the lag. MCLA profoundly inhibited the propagation reaction in Fe2+-dependent lipid peroxidation in liposomes, and MCLA disappearance accompanied by suppression of oxygen consumption markedly occurred in liposomes susceptible to peroxidation. Thiobarbituric acid-reactive substances in all systems used were also suppressed by MCLA dose dependently. These results indicate that MCLA has an antioxidant property through scavenging free radicals.
Division of Environmental Hygiene, Hokkaido College of Pharmacy, Japan. Tampo@hit.ac.jp
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Tampo
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Tsukamoto
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Yonaha
D000578:Amidines; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives; D011756:Diphosphates; D016166:Free Radical Scavengers; D007093:Imidazoles / Q000494:pharmacology*; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects*; D008081:Liposomes; D008163:Luminescent Measurements; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D015335:Molecular Probes; D010101:Oxygen Consumption; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000032:analysis; D017392:Thiobarbituric Acid Reactive Substances
D000578:Amidines; D000975:Antioxidants; D001555:Benzene Derivatives; D011756:Diphosphates; D016166:Free Radical Scavengers; D007093:Imidazoles; D008081:Liposomes; D015335:Molecular Probes; D011719:Pyrazines; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; C063412:2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one; C046728:2,2'-azobis(2-amidinopropane); D007501:Iron; C007164:cumene hydroperoxide; C049051:ferric pyrophosphate