Cloning and characterization of a new swine MHC (SLA) class II DQB allele.
Major histocompatibility complex (MHC) of pigs is known as swine leukocyte antigen (SLA). The cDNA encoding a new allele of SLA class II DQ beta-chain was successfully isolated from a CSK miniature pig (derived from Göttingen strain) and characterized by sequence analyses. SLA-DQB cDNA fragment encoding beta 2-domain was amplified by reverse transcriptase-polymerase chain reaction using the sequences preserved in a various vertebrates as primers. Using non-radioisotope technique with the PCR product as a probe, cDNA clone G01 was isolated from a spleen cDNA library, and nucleotide sequence of this clone was determined. This clone encompassed a whole SLA-DQ beta-chain coding region, containing a total length of 1161 nucleotides with an open reading frame (ORF) of 786 nucleotides, 5' untranslated region of 15 nucleotides, and 3' untranslated region of 360 nucleotides ending with a canonical polyadenylation signal, followed by a poly A tail. Sequence comparisons of the ORF of this clone with those of known SLA-DQB genes confirmed that this clone is a new allele (SLA-DQB*G01). Phylogenetic analysis of the nucleotide sequences of swine, human, and murine MHC class II genes indicated that SLA-DQB was more similar to HLA-DQB1 than H-2A beta. Comparison of the nucleotide and deduced amino acid sequences among SLA-DQB alleles showed that the SLA-DQ beta-chain polymorphism was found almost in beta 1-domain which contains the antigenic peptide binding sites.
Department of Molecular Immunology, School of Agriculture and Life Sciences, University of Tokyo, Japan.
T
T
Hosokawa
Y
Y
Tanioka
M
M
Tanigawa
Y
Y
Matsumoto
T
T
Onodera
Y
Y
Matsumoto
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D019143:Evolution, Molecular; D015723:Gene Library; D005802:Genes, MHC Class II; D006683:HLA-DQ Antigens / Q000096:biosynthesis / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006801:Humans; D008285:Major Histocompatibility Complex; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013154:Spleen / Q000276:immunology; D013552:Swine; D013556:Swine, Miniature / Q000235:genetics* / Q000276:immunology*
D018076:DNA, Complementary; D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C057615:HLA-DQbeta antigen