Expression of protein kinase C isoenzymes in cultured hooded rat retinal pigmented epithelial cells: comparison with dystrophic Royal College of Surgeons rat.
OBJECTIVE
To determine the isoenzymes of Protein Kinase C (PKC) that are present in cultured retinal pigmented epithelial (RPE) cells from both the Lister hooded rat and the Royal College of Surgeons (RCS) rat.


METHODS
PKC isoenzymes were determined using immunocytochemistry and electrophoresis / Western blotting.


RESULTS
Analyses showed the presence of six isoenzymes of PKC (PKCalpha, beta1, beta2, delta, epsilon and zeta) within cultured RPE cells derived from Lister hooded rats. Significantly, however, cultures of cells derived from dystrophic Royal College of Surgeons rats differed in PKCdelta and PKCalpha isoenzyme expression as compared with cultured hooded rat RPE cells.


CONCLUSIONS
Rat RPE cells express several PKC isoenzymes. Differences in expression of PKCalpha and PKCdelta in RCS rat RPE cells may relate to the decreased phagocytic capability of these cells as compared with those of the Lister hooded rat.
Nuffield Laboratory of Ophthalmology, University of Oxford, England, UK.
J P
JP
Wood
N N
NN
Osborne
D000818:Animals; D015153:Blotting, Western; D002478:Cells, Cultured; D004586:Electrophoresis; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D010857:Pigment Epithelium of Eye / Q000166:cytology / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains
D007527:Isoenzymes; D011493:Protein Kinase C