Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors.
We recently identified and characterized a novel murine gene, ENC-1, that is expressed primarily in the nervous system and encodes an actin-binding protein. To gain insight into a potential role for ENC-1 gene in the processes of cell differentiation and malignant transformation in the human nervous system, we first cloned and characterized the human homologue of ENC-1. The human ENC-1 gene appeared to be highly expressed in adult brain and spinal cord, and in a number of cell lines derived from nervous system tumors we detected low steady-state levels of ENC-1 mRNA. We used a neuroblastoma differentiation model, the retinoic acid-induced neuronal differentiation of SMS-KCNR cells, to study the regulation of the ENC-1 gene during neural crest cell differentiation. We found that the expression of ENC-1 increased dramatically in the differentiated SMS-KCNR cells as compared to control undifferentiated cells. These results suggest that ENC-1 expression plays a role during differentiation of neural crest cells and may be down regulated in neuroblastoma tumors.
Hernandez
M C
MC
Department of Neurological Surgery, School of Medicine, Brain Tumor Research Center, HSE 722, 513 Parnassus Avenue, San Francisco, California, 94143-0520, USA.
Andres-Barquin
P J
PJ
Holt
I
I
Israel
M A
MA
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D002455:Cell Division / Q000187:drug effects; D002490:Central Nervous System / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics* / Q000502:physiology; D008969:Molecular Sequence Data; D009423:Nervous System Neoplasms / Q000235:genetics* / Q000473:pathology; D009479:Neuropeptides; D009687:Nuclear Proteins; D010179:Pancreas / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013116:Spinal Cord / Q000378:metabolism; D014018:Tissue Distribution; D014212:Tretinoin / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000970:Antineoplastic Agents; D018076:DNA, Complementary; D008840:Microfilament Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D012333:RNA, Messenger; C112302:ectodermal-neural cortex 1 protein; D014212:Tretinoin; D003994:Bucladesine