Evidence for three-dimensional interlayer correlations in cationic lipid-DNA complexes as observed by cryo-electron microscopy.
A fingerprint-like pattern across multilamellar, lipid-DNA complexes is attributed to DNA condensed as parallel helices between lipid bilayers. It is argued that the patterning indicates the existence of 3-D correlation forces between DNA-covered bilayers, following the DNA-driven formation of multilamellar liposomes from unilamellar vesicles.
Battersby
B J
BJ
Medizinische Biophysik, Technische Universität München, Klinikum r. d.I., Ismaningerstr. 22, D-81675 Munich, Germany. battersb@chemistry.uq.edu.au
Grimm
R
R
Huebner
S
S
Cevc
G
G
D002412:Cations; D004247:DNA / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D005615:Freezing; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry; D008854:Microscopy, Electron
D002412:Cations; D008055:Lipids; D008081:Liposomes; D004247:DNA