Keratin 17 is expressed during the course of SLS-induced irritant contact dermatitis, but unlike keratin 16, the degree of expression is unrelated to the density of dividing keratinocytes.
The aims of this study were to utilize quantitative immunocytochemical techniques to determine the densities of keratin 16 (K16) and keratin 17 (K17) expressed by keratinocytes during the course of acute patch test reactions to sodium lauryl sulfate (SLS), and to relate these to the proliferative state of the epidermis, as assessed by Ki-67 immunolabelling. Significantly increased numbers of dividing keratinocytes were present in 48h and 96h reactions, concurrent with high levels of expression of K16 and more moderate expression of K17. Statistical analysis indicated a good correlation between K16 expression and the density of Ki-67+ keratinocytes present in the epidermis (r=0.843). This was not the case for K17 (r=0.396). The results demonstrate that both K16 and K17 expression are features of acute irritant contact dermatitis reactions, but suggest that the factors which influence and control their expression differ.
Department of Dermatology, Amersham Hospital, Bucks, UK.
C M
CM
Willis
L
L
Reiche
J D
JD
Wilkinson
D000328:Adult; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003627:Data Interpretation, Statistical; D017453:Dermatitis, Irritant / Q000209:etiology*; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D007633:Keratins / Q000032:analysis / Q000096:biosynthesis / Q000187:drug effects*; D008297:Male; D008875:Middle Aged; D010328:Patch Tests; D012967:Sodium Dodecyl Sulfate / Q000009:adverse effects / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000009:adverse effects / Q000494:pharmacology*
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D007633:Keratins