Fab-independent antiadhesion effects of secretory immunoglobulin A on S-fimbriated Escherichia coli are mediated by sialyloligosaccharides.
S-fimbriated Escherichia coli strains cause sepsis and meningitis in newborns and are known to recognize the carbohydrate sequence sialyl-(alpha2-3)-galactoside. We show that adhesion of cloned S-fimbriated E. coli to human epithelial cells is inhibited Fab independently by sialyloligosaccharides on secretory immunoglobulin A (s-IgA). This indicates an anti-infective function of s-IgA (Fc), particularly in early human milk.
Zentrum für Kinderheilkunde der Heinrich-Heine-Universität, Düsseldorf, Germany. schroten@uni-duesseldorf.de
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Schroten
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Stapper
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Plogmann
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Köhler
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Hacker
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Hanisch
D000328:Adult; D001422:Bacterial Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000378:metabolism*; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000378:metabolism* / Q000494:pharmacology; D007140:Immunoglobulin Fab Fragments / Q000378:metabolism*; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D008766:Methylmannosides / Q000494:pharmacology; D009439:Neuraminidase; D009844:Oligosaccharides / Q000378:metabolism*
D007071:Immunoglobulin A, Secretory; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D008766:Methylmannosides; D009844:Oligosaccharides; C016735:sialooligosaccharides; C008466:methylmannoside; D009439:Neuraminidase