The albumin of the brown trout (Salmo trutta) is a glycoprotein.
The albumin from an Atlantic salmonid, the brown trout (Salmo trutta), is 1730 Da higher in molecular mass than the albumin from a Pacific salmonid, the chinook salmon (Oncorhynchus tshawytscha), at 65230 Da. Digestion with neuraminidase revealed that purified brown trout albumin contained sialic acid while chinook salmon albumin did not. Concanavalin A-sepharose affinity chromatography was used to purify a glycopeptide from a total tryptic digest of brown trout albumin. The mass of this glycopeptide (3815 Da) was determined by mass spectrometry, and the sequence largely confirmed by N-terminal sequencing. The identified sequence of IAHCCNQSYSM-, contains an Asn-Gln-Ser glycosylation site and is identical to residues 475-485 derived from the cDNA of the albumin from the Atlantic salmon, the closest relative of the brown trout. Glycosylation of albumin is very unusual, and has not been identified in either reptilian or mammalian albumins. The finding of a glycoalbumin in salmonids, ancient members of the teleost fish subclass, coupled with evidence of albumin glycosylation in the oldest vertebrates, agnathans, as well as amphibians, suggests that albumin was originally a glycoprotein, but lost this modification sometime between the divergence of amphibians and reptiles.
Molecular Pathology Laboratory, Department of Pathology, Christchurch School of Medicine, University of Otago, P.O. Box 4345, Christchurch, New Zealand. vmetcalf@chmeds.ac.nz
V J
VJ
Metcalf
S O
SO
Brennan
G K
GK
Chambers
P M
PM
George
D000818:Animals; D019143:Evolution, Molecular; D006020:Glycopeptides / Q000302:isolation & purification; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D011499:Protein Processing, Post-Translational; D012474:Salmon; D016415:Sequence Alignment; D017421:Sequence Analysis; D012709:Serum Albumin / Q000737:chemistry*; D013045:Species Specificity; D014337:Trout; D014714:Vertebrates
D006020:Glycopeptides; D006023:Glycoproteins; D012709:Serum Albumin