Effects of human chorionic gonadotropin (hCG) and beta-hCG on oncostatin M release from human peripheral blood mononuclear cells in vitro.
The effects of human chorionic gonadotropin (hCG) as well as the beta-subunit of hCG (beta-hCG) in concentrations of 80,000/25,000, 500, 50, and 5 mIU/ml on in vitro release of oncostatin M (OSM) from resting or phytohaemagglutinin (PHA) activated human peripheral blood mononuclear cells (PBMC), were studied. The concentration of OSM was measured in supernatants of human PBMC by quantitative sandwich enzyme technique. The hCG in dilutions of 80,000 mIU/ml (p < 0.05) stimulated OSM secretion from PHA activated or resting (p < 0.001) PBMC, but at a concentration of 5.0 mIU/ml diminished OSM release from activated cell cultures (p < 0.05). The beta-hCG at concentrations of 25,000 mIU/ml diminished OSM secretion only from PHA-activated PBMC. The hCG and beta-hCG at very high concentrations modulated the secretion of OSM from human lymphoid cells.
Komorowski
J
J
Institute of Endocrinology, Medical University of Lódz, Poland.
Gradowski
G
G
Stepień
H
H
D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D018997:Chorionic Gonadotropin, beta Subunit, Human / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008213:Lymphocyte Activation; D008297:Male; D053683:Oncostatin M; D010455:Peptides / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology
D006063:Chorionic Gonadotropin; D018997:Chorionic Gonadotropin, beta Subunit, Human; D016207:Cytokines; C508351:OSM protein, human; D010455:Peptides; D010835:Phytohemagglutinins; D053683:Oncostatin M