Pheno- and genotyping of Rhodococcus equi isolated from faeces of healthy horses and cattle.
The present study was designed to comparatively investigate 21 Rhodococcus equi isolates from the faeces of clinically healthy horses and cattle. The isolates were identified by cultural and biochemical properties and by PCR analysis. The latter, targeted to the gene coding for the 16S ribosomal RNA, revealed a species specific PCR product. The isolates were further characterised by serotyping with two typing systems, by haemagglutination tests and by plasmid and virulence protein profiling. Among the 21 cultures, four cultures contained plasmids, two of the four cultures expressed 15-17 kDa virulence proteins, no cultures contained 20 kDA virulence proteins. The 21 cultures were further analysed by DNA-fingerprinting. This was performed by macrorestriction analysis of chromosomal DNA by pulsed-field gel electrophoresis (PFGE). The DNA-restriction patterns were different for most of the isolates indicating a clone heterogeneity among isolates from single farms. Serotyping, determination of virulence marker and PFGE analysis of R equi appeared to be useful for further characterisation of this species, possibly of importance for virulence estimation of single R equi isolates and for epidemiological studies.
Institut für Bakteriologie und Immunologie, Giessen, Germany.
I
I
Soedarmanto
W
W
Zhicai
A
A
Setyamahanani
C
C
Lämmler
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005243:Feces / Q000382:microbiology*; D005838:Genotype; D006386:Hemagglutination Tests; D006736:Horses / Q000382:microbiology*; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics / Q000302:isolation & purification; D016929:Rhodococcus equi / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D014774:Virulence
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S