Analysis of fumonisins and Alternaria alternata toxin by liquid chromatography-enzyme-linked immunosorbent assay.
Use of a direct competitive enzyme-linked immunosorbent assay (ELISA) as a postcolumn monitoring system after liquid chromatography (LC) is described for analysis of different fumonisin analogs. Without cleanup and derivatization, sample extracts are directly injected into a C18 reversed-phase column and then subjected to LC. Fractions (0.5 mL each) are collected and then analyzed by ELISA. LC using a water-methanol gradient separated the 3 major fumonisins FmB1, FmB2, and FmB3, and as low as 0.1 ng FmB1 could be detected. Recovery of FmB1 added to ground corn (100-1000 ng/g) and then extracted with CH3CN-H2O (1 + 1, v/v) was 78.8%. Analysis of fumonisins in one starch and 14 naturally contaminated corn samples showed that FmB1 was the major fumonisin. Ten samples also were contaminated with FmB2, but only 2 samples were contaminated with FmB3. The method also was used to analyze extracts from cultures of 3 Alternaria alternata (AAL) strains. Both FmB1 and the AAL toxin TA were detected in the culture extracts, and their amounts varied considerably with the cultures tested.
University of Wisconsin-Madison, Food Research Institute 53706, USA.
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D000528:Alternaria / Q000737:chemistry*; D002264:Carboxylic Acids / Q000032:analysis*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D037341:Fumonisins; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry
D002264:Carboxylic Acids; D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C073063:fumonisin B3; C056933:fumonisin B1