Growth stimulation of normal melanocytes and nevocellular nevus cells by gastrin releasing peptide (GRP).
In order to know the possible effects of gastrin releasing peptide (GRP) on nevus cells and melanocytes, we studied the effect of GRP on the proliferation of cultured human nevus cells and normal melanocytes. MTS assay showed that GRP stimulated the growth of viable melanocytes at 1000 ng/ml. GRP also stimulated the growth of nevus cells in a dose dependent manner and maximum stimulation was obtained at 100 ng/ml of GRP. GRP was less effective for growth stimulation of normal melanocytes than nevus cells. The cytoplasm of nevus cells were positively stained by polyclonal anti-GRP antibody. We also detected the expression of GRP and GRP receptor mRNAs in these cells by RT-PCR. These results suggest that GRP acts as an autocrine growth factor for nevus cells and normal melanocytes.
Department of Dermatology, Oita Medical University, Japan.
H
H
Terashi
S
S
Itami
T
T
Tadokoro
M
M
Takeyama
K
K
Katagiri
S
S
Takayasu
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019886:Gastrin-Releasing Peptide / Q000096:biosynthesis / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009506:Nevus / Q000378:metabolism / Q000473:pathology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D018004:Receptors, Bombesin / Q000096:biosynthesis; D013268:Stimulation, Chemical
D012333:RNA, Messenger; D018004:Receptors, Bombesin; D019886:Gastrin-Releasing Peptide