Differential expression of moesin in cells of hematopoietic lineage and lymphatic systems.
Moesin is a member of the ERM family consisting of ezrin, radixin, and moesin. The protein is located in the plasma membrane similarly to ezrin and radixin, and is thought to regulate cellular movements and morphological changes. Using monoclonal antibody CR-22, the specificity of which against human moesin was confirmed by immunoprecipitation and western blotting analysis, we immunohistochemically stained various formalin-fixed and paraffin-embedded human tissues, in particular, clots of bone marrow and lymphatic tissues, to examine moesin expression in cells of hematopoietic lineage and lymphatic systems. In the bone marrow, moesin was expressed in myeloid cells, while little staining was detected in erythroid cells. Moesin was highly expressed in both the center and the periphery of mature megakaryocytes. In the lymphatic tissues, moesin was strongly expressed by T-lymphocytes in the paracortex. In the mantle zone, the periphery of the germinal center, moesin was expressed by small lymphocytes which were identified as B-lymphocytes. Furthermore, in areas of inflammation, moesin was expressed in both the center and the periphery of neutrophils, whereas in some neutrophils in distant areas, moesin was localized at the cellular periphery. These results suggest that differential expression of moesin in these cells is involved in their morphology and specialized functions.
Department of Molecular Oncology and Angiology, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Nagano, Japan.
J
J
Masumoto
J
J
Sagara
M
M
Hayama
E
E
Hidaka
T
T
Katsuyama
S
S
Taniguchi
D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000378:metabolism; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000378:metabolism; D019070:Cell Lineage; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism*; D008840:Microfilament Proteins; D008854:Microscopy, Electron; D009504:Neutrophils / Q000378:metabolism; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D011506:Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000378:metabolism; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D008840:Microfilament Proteins; D011506:Proteins; C071058:moesin