A chiral HPLC method for the simultaneous separation of configurational isomers of the predominant cis/trans forms of astaxanthin.
We report an HPLC method that allows the simultaneous separation of configurational isomers of the predominant cis/trans forms of astaxanthin. The configurational isomers of the all-trans-, and most of the configurational isomers of the 9-cis-, 13-cis- and 15-cis-astaxanthin were separated on a Sumichiral OA-2000 column, which is manufactured and packed in Japan with a Pirkle covalent D-phenylglycine chiral stationary phase (CSP). The large separation of the cis isomers from the all-trans isomers that we report here ensure the suitability of this method for the routine determination of the ratio of the configurational isomers of all-trans-astaxanthin.
Office of Cosmetics and Colors, U.S. Food and Drug Administration, Washington, DC 20204, USA.
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Abu-Lafi
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Turujman
D000276:Adjuvants, Immunologic / Q000302:isolation & purification*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005998:Glycine / Q000031:analogs & derivatives; D015203:Reproducibility of Results; D012474:Salmon / Q000378:metabolism*; D012997:Solvents; D013237:Stereoisomerism; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000302:isolation & purification
D000276:Adjuvants, Immunologic; D012997:Solvents; D024341:Xanthophylls; D019207:beta Carotene; C008852:2-phenylglycine; C005948:astaxanthine; D005998:Glycine