Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes.
A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as Rhodococcus sp. The strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of E-pyridine-3-aldoxime to form 3-cyanopyridine. The enzyme activity was induced by various aldoximes and nitriles. The strain metabolized the aldoxime as follows: E-pyridine-3-aldoxime was dehydrated to form 3-cyanopyridine, which was converted to nicotinamide by a nitrile hydratase, and the nicotinamide was successively hydrolyzed to nicotinic acid by an amidase.
Kato
Y
Y
Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, 5180 Kurokawa, Kosugi, Toyama 939-03, Japan.
Ooi
R
R
Asano
Y
Y
D000581:Amidohydrolases / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D004790:Enzyme Induction / Q000502:physiology; D007700:Kinetics; D009536:Niacinamide / Q000378:metabolism; D009570:Nitriles / Q000378:metabolism*; D010091:Oximes / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012240:Rhodococcus / Q000201:enzymology*; D012988:Soil Microbiology
D001426:Bacterial Proteins; D009570:Nitriles; D010091:Oximes; D011725:Pyridines; D009536:Niacinamide; D000581:Amidohydrolases; C020729:3-cyanopyridine