Epstein-Barr virus recombinant lacking expression of glycoprotein gp150 infects B cells normally but is enhanced for infection of epithelial cells.
Glycoprotein gp150 is a highly glycosylated protein encoded by the BDLF3 open reading frame of Epstein-Barr virus (EBV). It does not have a homolog in the alpha- and betaherpesviruses, and its function is not known. To determine whether the protein is essential for replication of EBV in vitro, a recombinant virus which lacked its expression was made. The recombinant virus had no defects in assembly, egress, binding, or infectivity for B cells or epithelial cells. Infection of epithelial cells was, however, enhanced. The glycoprotein was sensitive to digestion with a glycoprotease that digests sialomucins, but no adhesion to cells that express selectins that bind to sialomucin ligands could be detected.
School of Biological Sciences, University of Missouri-Kansas City, Kansas City, Missouri 64110, USA.
C M
CM
Borza
L M
LM
Hutt-Fletcher
D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D019040:E-Selectin / Q000378:metabolism; D004847:Epithelial Cells / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics / Q000502:physiology*; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D016366:Open Reading Frames; D019007:P-Selectin / Q000378:metabolism; D010957:Plasmids; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000502:physiology*
D019040:E-Selectin; D006023:Glycoproteins; D019007:P-Selectin; D014764:Viral Proteins; D008666:Metalloendopeptidases; C070738:O-sialoglycoprotein endopeptidase