Hepatic blood flow and oxygen extraction ratio during normothermic recirculation and total body cooling as viability predictors in non-heart-beating donor pigs.
BACKGROUND
Our aim was to evaluate the hepatic blood flows and oxygen metabolism of non-heart-beating donor (NHBD) pigs, with the use of cardiopulmonary bypass (CPB) and normothermic recirculation (NR) before total body cooling, and its relationship with recipient survival.


METHODS
Thirty-five pigs were transplanted with an allograft from NHBDs. After warm ischemia (WI) time (20, 30, or 40 min), CPB and NR were run for 30 min. After this period, the animals were cooled to 15 degrees C. In the control group (20 min of WI), the period of NR was excluded. Liver procurement was then performed.


RESULTS
Survival rate was 100% in the 20WI, 70% in the 30WI, and 50% in the 40WI. Control group survival rate was 0%. Hepatic artery blood flow and portal blood flow recovered during NR. Pump blood flow during CPB increased rapidly during NR and was significantly higher in the 20WI. When donors of the livers transplanted in "surviving pigs" (DSP) were compared with donors of the livers transplanted in "nonsurviving pigs" (DNSP), hepatic artery blood flow, portal blood flow, and pump blood flow were higher in the DSP. Hepatic oxygen extraction ratio increased in the three groups with respect to baseline values. Hepatic oxygen extraction ratio was lower in the 20WI than in the other groups and was lower in the DSP than in the DNSP.


CONCLUSIONS
The use of a NR period before total body cooling improves survival of liver transplantation in NHBDs. Portal blood flow and pump blood flow measurements can predict the viability of the grafts.
Department of Anesthesiology, Hospital Clinic of Barcelona, Spain.
R
R
Valero
J C
JC
García-Valdecasas
J
J
Tabet
P
P
Taurá
R
R
Rull
J
J
Beltran
F
F
García
F X
FX
González
M A
MA
López-Boado
C
C
Cabrer
J
J
Visa
D000818:Animals; D002315:Cardiopulmonary Bypass; D008102:Liver Circulation; D016031:Liver Transplantation; D010100:Oxygen / Q000378:metabolism*; D015996:Survival Rate; D013552:Swine
D010100:Oxygen