The role of cytokines in cervical ripening: correlations between the concentrations of cytokines and hyaluronic acid in cervical mucus and the induction of hyaluronic acid production by inflammatory cytokines by human cervical fibroblasts.
OBJECTIVE
The purpose of our study was (1) to explain the relationship between levels of inflammatory cytokines and levels of hyaluronic acid in cervical mucus of pregnant women and (2) to investigate whether cytokines promote hyaluronic acid production by human cervical fibroblasts in vitro.


METHODS
The concentration of hyaluronic acid, interleukin-1beta, and interleukin-8 were measured in cervical mucus of pregnant women, and hyaluronic acid production by cytokine-treated (interleukin-1beta and interleukin-8) cultured fibroblasts was measured.


RESULTS
Hyaluronic acid concentrations in the mucus of pregnant women with threatened premature labor were higher than in mucus of normal pregnant women (P < .05). Correlations were found between hyaluronic acid concentrations and interleukin-1beta (P = .018) and interleukin-8 (P = .003) concentrations in cervical mucus. Cytokines (especially interleukin-8) stimulated hyaluronic acid production by cultured cervical fibroblasts.


CONCLUSIONS
Cytokines induce hyaluronic acid production by human cervical fibroblasts, which may promote cervical ripening.
Department of Obstetrics and Gynecology, Akita University School of Medicine, Japan.
M
M
Ogawa
H
H
Hirano
H
H
Tsubaki
H
H
Kodama
T
T
Tanaka
D000328:Adult; D002582:Cervix Mucus / Q000378:metabolism*; D002584:Cervix Uteri / Q000166:cytology / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism / Q000502:physiology*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis / Q000378:metabolism*; D007249:Inflammation / Q000503:physiopathology*; D007747:Labor Stage, First; D016014:Linear Models; D011247:Pregnancy
D016207:Cytokines; D006820:Hyaluronic Acid