Identification of four chitin synthase genes in the rice blast disease agent Magnaporthe grisea.
Four chitin synthase gene fragments were isolated from Magnaporthe grisea by use of polymerase chain reaction. A pair of degenerate primers based on conserved zymogen-type chitin synthase sequences amplified a approximately 600-bp product containing three chitin synthase gene fragments. A second pair of degenerate primers, based on conserved sequences between the chitin synthases 3 of Saccharomyces cerevisiae and Candida albicans, amplified a single 770-bp fragment. The four corresponding amino acid sequences each fell into one of the chitin synthase classes I-IV, as deduced from sequence analysis. Northern analysis demonstrated that the class II gene was expressed transiently in early phases of growth, whereas the class I and III genes as well as the class IV gene were expressed throughout the entire life cycle. Furthermore, the class III gene was the most expressed.
Vidal-Cros
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Laboratoire de Chimie Organique Biologique, URA CNRS 493, Paris, France. vidalc@ccr.jussieu.fr
Boccara
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D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002687:Chitin Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D002687:Chitin Synthase