Development of peptide vaccines inducing production of neutralizing antibodies against HIV-1 viruses in HLA-DQ6 mice.
Peptide vaccines against HIV-1 were prepared according to the cassette theory that we had proposed previously. An amino acid sequence of B subtype consensus of the HIV-1 V3 region was introduced into the MHC binding component with a supermotif for various MHC class II. The peptide vaccines induced T-cell responses in the DQ6 mice in which only DQ6 molecules were expressed as MHC class II. By contrast, an original V3 peptide including the consensus sequence was non-immunogenic in the DQ6 mice. Antibodies obtained from the DQ6 mice immunized with the peptide vaccines neutralized laboratory B subtype strains of HIV-1 in vitro. It may be anticipated that these peptide vaccines protect infection of HIV-1 in DQ6 positive individuals.
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
A
A
Takahashi
K
K
Ogasawara
N
N
Matsuki
K
K
Fujinaga
T
T
Nakaya
K
K
Ikuta
W
W
Auwanit
M
M
Honda
Y
Y
Fukui
T
T
Sasazuki
K
K
Iwabuchi
K
K
Onoé
D016915:AIDS Vaccines / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D000818:Animals; D015483:HIV Antibodies / Q000097:blood* / Q000276:immunology; D015497:HIV-1 / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000097:blood / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009500:Neutralization Tests; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000276:immunology / Q000494:pharmacology*
D016915:AIDS Vaccines; D015483:HIV Antibodies; D006683:HLA-DQ Antigens; C063831:HLA-DQ6 antigen; D014614:Vaccines, Synthetic