Characteristic genotypes discriminate between Babesia canis isolates of differing vector specificity and pathogenicity to dogs.
The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA gene were characterized in eight Babesia canis isolates of differing geographic origin, vector specificity, and pathogenicity to dogs. The genotypes determined by sequencing segregated into three clearly separated groups close to or near the species level and correspond to the previously proposed subspecies B. canis canis, B. canis vogeli, and B. canis rossi. The three genotypes can be distinguished by Sau96I digestion of the polymerase chain reaction (PCR)-amplified rDNA target.
Zahler
M
M
Institut für Vergleichende Tropenmedizin und Parasitologie, München, Germany. monika.zahler@lrx.uni-meunchen.de
Schein
E
E
Rinder
H
H
Gothe
R
R
D000818:Animals; D001096:Arachnid Vectors / Q000469:parasitology; D001403:Babesia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D001404:Babesiosis / Q000469:parasitology; D001483:Base Sequence; D016054:DNA, Protozoan / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs; D014644:Genetic Variation; D005838:Genotype; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012689:Sequence Homology, Nucleic Acid; D013987:Ticks / Q000469:parasitology
D016054:DNA, Protozoan; D004275:DNA, Ribosomal