Preparation of stereoregulated antisense oligodeoxyribonucleoside phosphorothioate and interaction with its complementary DNA and RNA.
Diastereoisomeric specificity of oligodeoxyribonucleoside phosphorothioate (OPT) in DNA/OPT and RNA/OPT hybrid formation was investigated. The difference in the configuration between RRRR and SSSS was reflected in the conformation and the stability of the DNA/OPT and RNA/OPT hybrids. Therefore, findings of this report rationalize the antisense effect by non-stereoregulated OPT and the difference of diastereoisomerism in susceptibility to RNase H.
Tamura
Y
Y
Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Miyoshi
H
H
Yokota
T
T
Makino
K
K
Murakami
A
A
D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism; D016373:DNA, Antisense / Q000235:genetics*; D003853:Deoxyribonucleosides / Q000138:chemical synthesis*; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis; D009843:Oligoribonucleotides / Q000138:chemical synthesis; D012313:RNA / Q000378:metabolism; D013237:Stereoisomerism; D013696:Temperature
D016373:DNA, Antisense; D003853:Deoxyribonucleosides; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012313:RNA; D004247:DNA