Multimodal application of lectin affinity electrophoresis of alpha-fetoprotein.
Multimodal application of lectin affinity electrophoresis of alpha-fetoprotein (AFP) glycoforms is reviewed. Crossed affinity immunoelectrophoresis developed by Bøg-Hansen and others was extended to tandem-lectin affinity electrophoresis by tandem lining of two different lectin gels and to mixed-lectin affinity electrophoresis. By introducing an antibody-affinity blotting technique for detection of separated glycoforms of AFP, several two-dimensional combinations of lectin affinity electrophoresis became possible: two different lectins for the first and second dimension electrophoresis, lectin-gradient affinity electrophoresis, and electrophoretic separation of lectin isoforms in the first-dimension electrophoresis, followed by affinity electrophoresis against the separated lectin isoforms. Usefulness of the different modalities of lectin affinity electrophoresis for several analytical purposes has been described.
Taketa
K
K
Department of Public Health, Okayama University Medical School, Japan. ibarahp@oka.urban.ne.jp
D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D037102:Lectins; D000509:alpha-Fetoproteins / Q000032:analysis*
D037102:Lectins; D000509:alpha-Fetoproteins