Biochemical characterization of MIP-1 alpha nuclear protein.
A family of hematopoietic specific transcription factors, MIP-1 alpha nuclear protein (MNP) family, has recently been identified. They are intimately involved in regulating the transcription of the huMIP-1 alpha gene in monocytes, T-cells, and transformed B-cells. One member of the family (MNP-1) is essential for promoter activity in monocytes and B-cells, while another (MNP-2) is required for full promotor activity in T-cells. A third member of the family (MNP-3) is expressed in PMA induced HL60 cells and probably has a role in monocyte differentiation. In this communication we demonstrate by two techniques that MNP-1 and MNP-2 are distinct but related factors, and we present further evidence to show that MNP-1 acts as a heterodimer.
Sharma
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Department of Biology, University of West Florida, Pensacola 32514, USA. vsharma@uwf.edu
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Ritter
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D001483:Base Sequence; D001665:Binding Sites; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000378:metabolism; D054407:Chemokine CCL4; D019175:DNA Methylation; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009000:Monocytes / Q000166:cytology / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D013601:T-Lymphocytes; D014157:Transcription Factors / Q000302:isolation & purification / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D054407:Chemokine CCL4; C114015:MNP-1 protein, human; C114016:MNP-2 protein, human; D019402:Macrophage Inflammatory Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors