Preferential relative porphyrin enrichment in solar keratoses upon topical application of delta-aminolevulinic acid methylester.
Topically applied delta-aminolevulinic acid is used efficiently for the treatment of solar keratoses by photodynamic therapy. Recent animal studies suggest that porphyrin sensitization of epithelial tissue is improved by using esters rather than free delta-aminolevulinic acid. The present study examines porphyrin metabolite formation after topical application of delta-aminolevulinic acid or delta-amino-levulinic acid methylester in human solar keratoses versus adjacent normal skin. Level of total porphyrins, porphyrin metabolites and protein were measured in skin samples excised after 1 and 6 h. Higher levels of porphyrins were observed in solar keratoses than in normal skin with both substances. Maximum porphyrin levels were present in solar keratoses treated with delta-aminolevulinic acid for 6 h. However, the ratio of porphyrins in solar keratoses versus adjacent normal skin was higher with delta-aminolevulinic acid methylester. The pattern of porphyrins showed no significant difference between normal and afflicted skin, protoporphyrin being predominant. The results suggest that application of free delta-aminolevulinic acid may be more effective in sensitizing solar keratoses. However, treatment with delta-aminolevulinic acid methylester leads to a preferential enrichment of porphyrins within lesional skin.
Institute of Physiological Chemistry, Heinrich-Heine-University, Düsseldorf, Germany.
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Fritsch
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Homey
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Stahl
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Lehmann
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Ruzicka
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Sies
D000287:Administration, Topical; D000622:Aminolevulinic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives*; D000818:Animals; D006801:Humans; D066298:In Vitro Techniques; D007642:Keratosis / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism*; D018462:Photobiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000008:administration & dosage*; D011166:Porphyrins / Q000378:metabolism*; D013472:Sunlight / Q000009:adverse effects
D017319:Photosensitizing Agents; D011166:Porphyrins; C114767:delta-aminolevulinic acid methyl ester; D000622:Aminolevulinic Acid