Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation.
Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
Barre
F X
FX
Cancer, CNRS UPR9079 Institut Gustave Roussy, Villejuif, France.
Mir
L M
LM
Lécluse
Y
Y
Harel-Bellan
A
A
D000595:Amino Acid Sequence; D001483:Base Sequence; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics; D018014:Gene Transfer Techniques; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D015003:Yeasts / Q000166:cytology / Q000235:genetics*
D005656:Fungal Proteins; D009841:Oligonucleotides