The angiogenic effect of tissue factor on tumors and wounds.
We report a novel function of tissue factor (TF) as an angiogenic factor in malignant and non-malignant cells and tissue. When methylcholanthrene A-induced murine fibrosarcoma (Meth-A sarcoma) was stably transfected with mouse TF (mTF) cDNA (pXT1 expression vector), its vascularization in vivo was significantly enhanced, whereas TF-antisense suppressed the vascularization and tumor growth. In vitro expression of vascular endothelial growth factor (VEGF) was enhanced with stable transfection of mTF (pcDNA3 expression vector) into a mouse fibroblast cell line (NIH3T3). Moreover, in vivo topical transfection of mTF (pcDNA3) showed an enhanced vascularization and healing in a diabetic mouse wound-healing model. This effect of TF as an angiogenic factor might be useful as an antitumor therapy against hypervascular tumors or as a novel agent against delayed wound healing.
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan.
K
K
Nakagawa
Y
Y
Zhang
H
H
Tsuji
M
M
Yoshizumi
T
T
Kasahara
H
H
Nishimura
P P
PP
Nawroth
M
M
Nakagawa
D016475:3T3 Cells; D000818:Animals; D016228:Endothelial Growth Factors / Q000502:physiology; D005354:Fibrosarcoma / Q000098:blood supply* / Q000235:genetics; D008222:Lymphokines / Q000502:physiology; D051379:Mice; D016688:Mice, Inbred NOD; D009374:Neoplasms, Experimental / Q000098:blood supply* / Q000235:genetics; D009389:Neovascularization, Pathologic; D013925:Thromboplastin / Q000502:physiology*; D014162:Transfection; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D014945:Wound Healing
D016228:Endothelial Growth Factors; D008222:Lymphokines; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D013925:Thromboplastin