Effect of prolonged staining with hematoxylin on detecting Helicobacter pylori in hematoxylin-eosin-stained gastric mucosa.
Helicobacter pylori (H. pylori) is identifiable in hematoxylin-eosin (HE)-stained preparations, particularly under a prolonged hematoxylin staining condition. The staining intensity of H. pylori bodies was increased when the hematoxylin staining period was doubled from 5 min, the routine period of Meyer's hematoxylin staining, to 10 min. The staining intensity of the background epithelial nuclei was only mildly increased. Hematoxylin-eosin staining employing a prolonged hematoxylin staining period gave detectability of H. pylori comparable to May-Giemsa and immunoperoxidase staining. The modified HE method is thus very useful and practical for identifying H. pylori in routine gastric biopsy specimens.
Tazawa
K
K
Department of Pathology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Tsutsumi
Y
Y
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001399:Azure Stains; D001706:Biopsy; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005189:False Positive Reactions; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis* / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D013194:Staining and Labeling / Q000379:methods
D001399:Azure Stains; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin