Capped mRNA degradation intermediates accumulate in the yeast spb8-2 mutant.
mRNA in the yeast Saccharomyces cerevisiae is primarily degraded through a pathway that is stimulated by removal of the mRNA cap structure. Here we report that a mutation in the SPB8 (YJL124c) gene, initially identified as a suppressor mutation of a poly(A)-binding protein (PAB1) gene deletion, stabilizes the mRNA cap structure. Specifically, we find that the spb8-2 mutation results in the accumulation of capped, poly(A)-deficient mRNAs. The presence of this mutation also allows for the detection of mRNA species trimmed from the 3' end. These data show that this Sm-like protein family member is involved in the process of mRNA decapping, and they provide an example of 3'-5' mRNA degradation intermediates in yeast.
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA.
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Boeck
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Lapeyre
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Brown
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Sachs
D000595:Amino Acid Sequence; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017353:Gene Deletion; D005838:Genotype; D007700:Kinetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids; D039102:Poly(A)-Binding Proteins; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps / Q000378:metabolism*; D012331:RNA, Fungal / Q000096:biosynthesis / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic
D005656:Fungal Proteins; C114069:LSM1 protein, S cerevisiae; D039102:Poly(A)-Binding Proteins; D039381:RNA Cap-Binding Proteins; D012315:RNA Caps; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins