Nicotine induced up-regulation of nicotinic receptors in CD-1 mice demonstrated with an in vivo radiotracer: gender differences.
Up-regulation of brain nicotinic receptors (nAChRs) by chronic nicotine treatment has chiefly been demonstrated by in vitro binding assays. Here, we report that up-regulation of nAChRs in CD-1 mice can be detected using a specific in vivo radioligand for nAChRs, [125I]IPH. After 10 days of (-)-nicotine administration, male and female mice demonstrated a significant elevation of [125I]IPH binding in all brain regions studied. [125I]IPH uptake also displayed significant gender differences with male animals showing a more pronounced increase in [125I]IPH accumulation.
Department of Radiology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA.
T
T
Mochizuki
V L
VL
Villemagne
U
U
Scheffel
R F
RF
Dannals
P
P
Finley
Y
Y
Zhan
H N
HN
Wagner
J L
JL
Musachio
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000493:pharmacokinetics; D005260:Female; D007457:Iodine Radioisotopes; D008297:Male; D051379:Mice; D009538:Nicotine / Q000494:pharmacology*; D018722:Nicotinic Agonists / Q000493:pharmacokinetics; D011725:Pyridines / Q000493:pharmacokinetics; D011869:Radioligand Assay; D011978:Receptors, Nicotinic / Q000096:biosynthesis*; D012727:Sex Characteristics; D015854:Up-Regulation / Q000187:drug effects*
D019086:Bridged Bicyclo Compounds, Heterocyclic; D007457:Iodine Radioisotopes; D018722:Nicotinic Agonists; D011725:Pyridines; D011978:Receptors, Nicotinic; D009538:Nicotine; C082748:epibatidine