[Effect of surfactants on peroxidase activity. II. Effect of cationic surfactants].
The effect of various cationic surfactants on the initial rate of the 5-aminosalicylic acid peroxidation with horseradish peroxidase was studied. With increasing concentration of the surfactant, the rate first increased and then decreased. We attributed these changes to a primary activation of the enzyme and/or an increase in the concentration of the substrate in the region of the active site and to the subsequent inactivation of the protein with surfactant followed by its denaturing, respectively.
Davletshin
A I
AI
Skryabin State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia.
Kalabina
N A
NA
Zaĭtsev
S Iu
SIu
Egorov
V V
VV
D002412:Cations; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019804:Mesalamine / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*
D002412:Cations; D004791:Enzyme Inhibitors; D008823:Micelles; D013501:Surface-Active Agents; D019804:Mesalamine; D006735:Horseradish Peroxidase