Triplet pregnancy after intracytoplasmic sperm injection of cryopreserved oocytes: case report.
OBJECTIVE
To report a triplet pregnancy that occurred after intracytoplasmic injection of sperm into cryopreserved oocytes.


METHODS
Case report.


METHODS
Instituto de Ginecología y Fertilidad (IFER), Buenos Aires, Argentina.


METHODS
A 36-year-old infertile patient with premature ovarian failure and a previous term pregnancy with fresh donated oocytes.


METHODS
We administered leuprolide acetate for pituitary down-regulation followed by E2 valerianate in incremental doses until an endometrial lining of >8 mm was observed by ultrasound. Thawing of frozen donated oocytes, intracytoplasmic sperm injection (ICSI), and translaparoscopic fallopian tube ET also were performed. Natural micronized progesterone was administered intravaginally (600 mg/d) before ET.


METHODS
Ultrasound at the 8th week of gestation revealed a triplet pregnancy with active fetal heartbeats.


RESULTS
A triple intrauterine gestation was achieved with the use of microinjection into cryopreserved oocytes.


CONCLUSIONS
This case illustrates the feasibility of oocyte cryopreservation for clinical use in the era of ICSI.
Instituto de Ginecología y Fertilidad (IFER), Buenos Aires, Argentina. IFER@COMNET.COM.AR
E
E
Young
A
A
Kenny
E
E
Puigdomenech
G
G
Van Thillo
M
M
Tiverón
A
A
Piazza
D000328:Adult; D015925:Cryopreservation; D003593:Cytoplasm; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000628:therapy*; D008297:Male; D008845:Microinjections; D009865:Oocytes; D011247:Pregnancy; D011272:Pregnancy, Multiple; D013084:Sperm-Ovum Interactions; D014310:Triplets