Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
D
D
Ramaiah
T
T
Koch
H
H
Orum
G B
GB
Schuster
D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000737:chemistry; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism*; D010777:Photochemistry; D011196:Potassium Permanganate; D011740:Pyrimidine Dimers / Q000032:analysis*; D017422:Sequence Analysis, DNA
D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D011196:Potassium Permanganate; D004255:Deoxyribodipyrimidine Photo-Lyase