[Dynamics of cell wall formation from Trichophyton mentagrophytes protoplast].
There is no reference to the cell wall formation during the regeneration process in dermatophyte. Having succeeded in identifying the cell wall formation system of protoplast from Trichophyton mentagrophytes I introduce here the dynamics of the regeneration of this protoplast and the formation of cell wall using ultrahigh resolution low-voltage scanning electron microscopy and transmission electron microscopy (TEM), compare to the formation of yeast cell wall. Protoplasts were formed from mycelia of T. mentagrophytes; they regenerated the cell wall substances and were followed by a network of fibrils after 4-6 hr, which developed into mature mycelia after 21 hr. A network of twisted microfibrils and rope-shaped thick fiber was seen; it was covered with a sheath-shaped structure and was created on the surface of the developing mycelium. Intrafibrillar space and the surface of thick fibers were gradually filled with amorphous particles and a mature cell wall surface was formed. This protoplast regeneration was completely inhibited by the antifungal agent, lanoconazole (2 ng/ml ). The sectioned TEM image revealed that normal mycelium having a thin cell wall developed from the abortive bead-shaped mycelium which was visible in the lose fibrous structure. Based on this result and our previos work on the ultrastructure of the mycelial surface of T. rubrum with lanoconazole, a model of the cell wall composition of Trichophyton is proposed.
Division of Material and Biological Function Science, Graduate School of Science, Japan Women's University, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo, 112-8681 Japan.
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Osumi
D002473:Cell Wall / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D011523:Protoplasts / Q000648:ultrastructure*; D012038:Regeneration; D014249:Trichophyton / Q000502:physiology / Q000648:ultrastructure*