Decreasing extracellular pH increases CD13 receptor surface content and alters the metabolism of HL60 cells cultured in stirred tank bioreactors.
Flow cytometry and Northern blotting were used to examine the effects of extracellular pH on CD13 receptor surface content and mRNA levels of HL60 (human promyelocytic leukemia) cells. Decreasing culture pH (7.4, 7.2, and 7.0) increased the CD13 receptor surface content of HL60 cells cultured at low agitation intensity (80 rpm) in 2-L bioreactors. Unlike our earlier findings on the effects of increasing agitation rates and serum concentrations, changes in CD13 receptor content in response to decreasing culture pH did not correlate with changes in CD13 mRNA levels. Decreasing culture pH also decreased the average HL60 cell size. HL60 cells cultured at pH 7.0 and 7.2 exhibited glucose consumption and lactate production rates that were approximately 30-40% and 20-30% lower, respectively, than values of cells cultured at pH 7.4.
McDowell
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Northwestern University, Department of Chemical Engineering, 2145 Sheridan Road E136, Evanston, Illinois 60208-3120, USA.
Papoutsakis
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D019149:Bioreactors; D015152:Blotting, Northern; D018826:CD13 Antigens / Q000235:genetics / Q000378:metabolism; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division / Q000502:physiology; D048429:Cell Size; D005110:Extracellular Space / Q000737:chemistry*; D005434:Flow Cytometry; D005947:Glucose / Q000378:metabolism; D018922:HL-60 Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000096:biosynthesis* / Q000378:metabolism
D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D019344:Lactic Acid; D018826:CD13 Antigens; D005947:Glucose