Evidence for the presence of a kininogen-like species in a case of total deficiency of low and high molecular weight kininogens.
Low and high molecular weight kininogens (LK and HK), containing 409 and 626 amino acids with masses of approximately 65 and 120 kDa after glycosylation, respectively, are coded by a single gene mapped to the human chromosome 3 by alternative splicing of the transcribed mRNA. The NH2-termini Glu1-Thr383 region, identical in LK and HK, contains bradykinin (BK) moieties Arg363-Arg371. LK, HK and their kinin products Lys-BK and BK are involved in several biologic processes. They are evolutionarily conserved and only 7 patients, all apparently normal, have been reported to lack them. In one of these patients (Williams' trait), a codon mutation (Arg178-->stop) has been blamed for the absence of LK and HK. However, using Western blots with 2 monoclonal anti-HK antibodies, one that recognizes the region common to LK and HK and the other that recognizes only HK, 1 detected approximately 110-kDa bands in the plasma of this LK/HK-deficient patient vs approximately 120-kDa bands in normal human and ape plasmas. With polyclonal anti-Lys-BK antibody, which strongly detects BK cleaved at its COOH-terminus in purified HK, 1 detected approximately 110-kDa bands in the normal and the deficient plasmas. Western blots with a monoclonal anti-prekallikrein (PK) antibody showed that surface activation of PK and distribution of PK activation products, both dependent on HK, were similar in these plasmas. These findings suggest that a mutant gene yielded a kininogen-like species possibly involving aberrant mRNA splicing-structurally different from normal HK, but apparently with the capacity to carry out seemingly vital HK functions.
Department of Psychiatry and Behavioral Sciences, University of Texas at Houston 77030, USA. dveloso@msi66.msi.uth.tmc.edu
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D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D001778:Blood Coagulation Disorders / Q000235:genetics* / Q000276:immunology; D015153:Blotting, Western; D005260:Female; D006801:Humans; D019679:Kininogen, High-Molecular-Weight / Q000235:genetics* / Q000276:immunology; D019728:Kininogen, Low-Molecular-Weight / Q000235:genetics* / Q000276:immunology; D007704:Kininogens / Q000235:genetics*; D007705:Kinins / Q000302:isolation & purification; D008297:Male; D009154:Mutation; D010949:Plasma / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics
D000911:Antibodies, Monoclonal; D019679:Kininogen, High-Molecular-Weight; D019728:Kininogen, Low-Molecular-Weight; D007704:Kininogens; D007705:Kinins; D012333:RNA, Messenger