Expression of glucocorticoid receptor mRNA and protein in the olfactory mucosa: physiologic and pathophysiologic implications.
OBJECTIVE
Define the presence and distribution of glucocorticoid receptors (GRs) within the olfactory mucosa in order to assess potential physiologic and pathophysiologic effects of these hormones on olfaction.


METHODS
The olfactory mucosa was harvested from adult male rats and guinea pigs. Kidney tissue was utilized as a known positive control.


METHODS
The techniques of reverse transcriptase-polymerase chain reaction (RT-PCR) and immunocytochemistry were utilized to examine the expression of GR mRNA and protein. To assure the presence of olfactory mucosa in the nasal tissue samples, RT-PCR was utilized to identify the olfactory marker protein (OMP).


RESULTS
The presence of GR mRNA was confirmed in both the olfactory mucosa and kidney. GR-like immunoreactivity associated with the olfactory epithelium was greatest at the apical surface, a position corresponding to the dendrites, knobs, and cilia of olfactory receptor neurons, as well as the supranuclear region of sustentacular cells. Weaker GR-like immunoreactivity was associated with the region of the cell bodies of the olfactory receptor neurons. Within the lamina propria, acinar cells of the Bowman's glands and olfactory nerve bundles were intensely immunoreactive.


CONCLUSIONS
The presence of GR mRNA and protein within the olfactory mucosa is consistent with a functional role for glucocorticoid hormones in the systemic regulation of olfaction. Furthermore, these studies suggest that corticosteroid medications may have direct effects on the cells of the olfactory mucosa in the pathologic state. The potential mechanisms whereby these hormones may act are discussed.
Department of Otolaryngology--Head and Neck Surgery, Northwestern University, Chicago, Illinois, USA.
A M
AM
Robinson
R C
RC
Kern
J D
JD
Foster
K J
KJ
Fong
D Z
DZ
Pitovski
D000818:Animals; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009831:Olfactory Mucosa / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D017208:Rats, Wistar; D011965:Receptors, Glucocorticoid / Q000032:analysis* / Q000235:genetics
D012333:RNA, Messenger; D011965:Receptors, Glucocorticoid; D012194:RNA-Directed DNA Polymerase