Characterization of an ammonium transport protein from the peribacteroid membrane of soybean nodules.
Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4<SUP ARRANGE="STAGGER">+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host.
Kaiser
B N
BN
Division of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia.
Finnegan
P M
PM
Tyerman
S D
SD
Whitehead
L F
LF
Bergersen
F J
FJ
Day
D A
DA
Udvardi
M K
MK
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary; D007473:Ion Channels / Q000378:metabolism; D007700:Kinetics; D008744:Methylamines / Q000378:metabolism; D008969:Molecular Sequence Data; D015388:Organelles / Q000378:metabolism; D018408:Patch-Clamp Techniques; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D011188:Potassium / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D013104:Spheroplasts / Q000378:metabolism; D013559:Symbiosis; D014170:Transformation, Genetic
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D007473:Ion Channels; D008744:Methylamines; D000644:Quaternary Ammonium Compounds; C114002:SAT1 protein, glycine max; D030262:Soybean Proteins; C027451:methylamine; D011188:Potassium