A trial to enhance the efficiency of Cyclosporin A in the treatment of Schistosoma mansoni infection by immunization of mice.
Cyclosporin A (CsA) acts in two distinct modes against Schistosoma mansoni (S. mansoni) infection. It behaves in part as an antihelminthic and also as an immunomodulator. In this study, two methods were performed to increase the efficacy of the subcurative dose of CsA (30 mg/kg). These methods were, passive immunization where the drug was given simultaneously with rabbit antisera post infection and active immunization where the worm surface antigen was given prior to infection. The results showed that both methods led to enhancement of the curative effect of the CsA. However, the active immunization gave more significant reduction in liver worm and egg loads. Histopathological study showed small size and few number of hepatic granulomas in the passively immunized group while in the active group, there was just few and small collection of inflammatory cells. Moreover, the indirect fluorescent antibody test visualized damage to the worm tegument in both types of immunization. Thus, it could be concluded that active immunization increased the immunomodulatory effect of CsA in treatment of S. mansoni with less toxic effects by using a subcurative dose.
Department of Parasitology, Faculty of Medicine, Alexandria University, Egypt.
E D
ED
Elkerdany
E R
ER
Ebrahim
A Y
AY
Negm
D000818:Animals; D000909:Antibodies, Helminth / Q000008:administration & dosage / Q000276:immunology; D000947:Antigens, Helminth / Q000008:administration & dosage / Q000276:immunology; D003131:Combined Modality Therapy; D016572:Cyclosporine / Q000627:therapeutic use*; D007116:Immunization, Passive; D051379:Mice; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000188:drug therapy / Q000276:immunology / Q000628:therapy*; D012556:Schistosomicides / Q000627:therapeutic use*; D014611:Vaccination
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D012556:Schistosomicides; D016572:Cyclosporine