[Changes in the antioxidant system of the aqueous humor, lens and erythrocytes after sulfur hexafluoride application to the vitreous of rabbits].
OBJECTIVE
To determine the activity of superoxide dismutase, catalase and the concentration of malondialdehyde (MDA) in aqueous humor, lens and red blood cells after application of sulfur hexafluoride (SF6) into vitreous of rabbits.


METHODS
0.5 ml of 100% SF6 was injected into the vitreous of 24 rabbits of New Zealand race. The animals were randomly divided into 3 groups (of 8 rabbits each) depending on the observation day: group 1-2nd day of experiment, group 2-7th day and group 3-14th observation day. The control group (gr. 0) consisted of 6 rabbits that did not undergo any operations. Activity of superoxide dismutase, catalase and MDA concentration were determined in aqueous humor, lens and systemic blood erythrocytes.


RESULTS
On the 7th day of observation an increased activity of dismutase and catalase as well as simultaneous increased MDA concentration were observed. In the lens on the 7th day the increased activity of dismutase was significant in relation to the results in the next time interval, whereas MDA concentration was significantly lower in all time intervals of the experiment in comparison with control group. In erythrocytes an increased activity of catalase was noticed on the 2nd and 14th day.


CONCLUSIONS
Increased occurrence of active oxygen species in aqueous humor leads to insufficiency of the antioxidant system and intensification of peroxidation processes, which is reflected by increased MDA concentration. However, in the lens of this experimental model a slight stimulation of antioxidant system by a small number of free radicals is observed, which provokes a reaction of sweeping them away. Efficiency of lens antioxidant system is secured by weakening of peroxidation processes, which is expressed in minimal drop of MDA concentration.
Kliniki Okulistycznej Szpitala Klinicznego WAM w Lodzi.
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Sztarbała
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Goś
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Kedziora
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Błaszczyk
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Sibińska
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Góralczyk
D000818:Animals; D001082:Aqueous Humor / Q000187:drug effects* / Q000378:metabolism; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D011817:Rabbits; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013459:Sulfur Hexafluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014822:Vitreous Body / Q000187:drug effects* / Q000378:metabolism
D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D013459:Sulfur Hexafluoride