Cloning and sequence analysis of a Trypanosoma cruzi alpha-tubulin cDNA.
A cDNA clone derived from the Trypanosoma cruzi alpha-tubulin gene was isolated and sequenced (Tc alpha tub; L37345). Tc alpha tub revealed an 87.79% and an 85.36% identity with the DNA sequence of T. brucei and Leishmania, respectively. This clone was used to study, by Northern blots, alpha-tubulin gene expression in epimastigotes, cell-cultured derived trypomastigotes and extracellular amastigotes. alpha-tubulin MRNA levels were the same in epimastigotes and trypomastigotes, however, there was a drastic decrease in amastigotes. This clone could be useful to elucidate the regulatory mechanisms of alpha-tubulin gene expression during the differentiation of T. cruzi.
Departamento de Genética Molecular, Universidad Central de Venezuela, Caracas, Venezuela.
M J
MJ
Gonzalez-Pino
R
R
Rangel-Aldao
T C
TC
Slezynger
D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification*; D015870:Gene Expression / Q000235:genetics*; D009711:Nucleotides / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014349:Trypanosoma cruzi / Q000235:genetics*; D014404:Tubulin / Q000235:genetics*
D018076:DNA, Complementary; D009711:Nucleotides; D012333:RNA, Messenger; D014404:Tubulin