The chemokine interleukin-8 regulates parathyroid secretion.
Interleukin-8 (IL-8) is a chemokine important in inflammatory processes. Homology cloning experiments performed using bovine parathyroid cDNA and degenerate primers encoding transmembrane regions III and VI of peptide and protein hormone G-protein coupled receptors identified a set of known receptors not previously identified in the parathyroid. Among these was the IL-8 type B receptor. Incubation of freshly isolated bovine parathyroid cells with recombinant IL-8 for 6-48 h produced an increase in the levels of mRNA for parathyroid hormone (PTH). The levels of PTH secreted in response to nanomolar amounts of IL-8 were also elevated in cells incubated for 1 h with IL-8. Differential display analysis of mRNA from parathyroid cells, incubated in the presence and absence of IL-8, permitted the identification of cDNA clones for RNA species whose expression was either elevated or suppressed. These experiments suggest that IL-8 and inflammatory events play a role in bone homeostasis through actions on the parathyroid gland.
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
R H
RH
Angeletti
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T
D'Amico
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S
Ashok
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J
Russell
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000032:analysis; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000494:pharmacology*; D010280:Parathyroid Glands / Q000187:drug effects* / Q000378:metabolism; D010281:Parathyroid Hormone / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D019707:Receptors, Chemokine / Q000032:analysis / Q000378:metabolism; D018123:Receptors, Interleukin / Q000032:analysis / Q000378:metabolism; D023063:Receptors, Interleukin-8B; D011994:Recombinant Proteins / Q000494:pharmacology
D018076:DNA, Complementary; D016209:Interleukin-8; D010281:Parathyroid Hormone; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D018123:Receptors, Interleukin; D023063:Receptors, Interleukin-8B; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins