Cloning and characterization of a microsatellite in the mitochondrial control region of the African side-necked turtle, Pelomedusa subrufa.
The nucleotide sequence of the African side-necked turtle mitochondrial control region and its flanking tRNA genes was determined. This 73% A+T-rich region is 1194 bp long. Several conserved motifs involved in the regulation of the mitochondrial genome replication process, including one conserved sequence block (CSB1), and three termination-associated sequences were identified. The most remarkable feature found in this control region was the presence of six microsatellite-containing tandem repeats between the CSB1 motif and the tRNAPhe gene. The potential usefulness of this microsatellite sequence for population-level studies is enhanced by its unique localization in the maternally inherited mitochondrial molecule.
Zardoya
R
R
Department of Ecology and Evolution and Program in Genetics, State University of New York, Stony Brook, NY 11794-5245, USA.
Meyer
A
A
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000235:genetics*
D004272:DNA, Mitochondrial; D005819:Genetic Markers