[Determination enzyme protein of CK-MB m-AST and ChE by immunological methods and survey of its applying values].
In recent decades, because considerable progress has been made due to rapid developments in basic theory and techniques in molecular biology and immunology, the determination of trace enzyme proteins is not difficult. We measured the serum concentration of Creatine kinase-MB (CK-MB) mitochondria aspartate aminotransferase (m-AST) and cholinesterase (ChE) immunologically and compared these findings with those of an assay of enzyme activity. Purification of enzyme protein and preparation of serum antibodies monoclonal antibodies established the immunological assay methods. Equipment and reagents for enzyme activity test use 7150 Biochemical Analyzer. CK-NAC AST and ChE were produced by trace kits (Australia). CK-MB and m-AST use immunological inhibition method. CK-MB m-AST ChE of protein determination used immunological turbidimetry. The normal group included 150 cases and the 1990 patient group. Results of the two methods did not significantly differ for normal controls, but were significantly different in the patient group. These results demonstrated that the two methods differ, although each may have specific clinical significance. How to evaluate these differences needs to be studied further, but immunological assay uses higher values for clinical diagnosis than enzyme activity assay.
Department of laboratory diagnosis, Norman Bethune University of Medical Sciences, Chang Chun P R of China.
X
X
Kang
B
B
Sun
S
S
Sun
W
W
Hou
F
F
Xie
M
M
Rong
R
R
Sun
D000911:Antibodies, Monoclonal; D001219:Aspartate Aminotransferases / Q000032:analysis*; D004796:Clinical Enzyme Tests; D003402:Creatine Kinase / Q000097:blood*; D006801:Humans; D007118:Immunoassay; D007527:Isoenzymes; D008107:Liver Diseases / Q000175:diagnosis; D008928:Mitochondria / Q000201:enzymology; D009203:Myocardial Infarction / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D007527:Isoenzymes; D001219:Aspartate Aminotransferases; D003402:Creatine Kinase