[Comparative study of the proliferation (by detection of Ki-67 antigen) and activity of nucleolar organizer regions in breast cancer cells].
The rates of proliferation, determined on the basis of antigen Ki-67 detection, and activity of nucleolar organizer regions (AgNORs) were compared in 10 benign lesions and 48 breast cancers (mostly invasive ductal carcinomas). Antigen Ki-67 was detected by the indirect immunoperoxidase method using polyclonal rabbit antibodies. Proteins of AgNORs were silver-stained after W.M. Howell and D.A. Black (modified by N.N.Mamayev). The fraction of Ki-67-positive cells--very close to proliferative pool--was 4.75 (1.60-11.76)% in benign tumors and 24.89 (8.64-65.16)%--in breast cancer, respectively. There was a significant correlation between Ki-67 expression and degree of histologically malignancy of breast tumors. There was a high correlation between Ki-67 expression in primary tumors and their metastases to regional lymph nodes; however, in metastases, the number of Ki-67-positive cells was significantly lower than in primary tumor. Also, the study established a distinct relationship between such indices of AgNOR activity as numbers of intra- and extranucleolar granules of silver and their total number in nucleus, on the one hand, and index of cell proliferation, on the other. The former represented specific clinicoanatomical features of tumor.
Prof. N.N.Petrov Research Institute of Oncology, Ministry of Health of the RF, St.Petersburg.
A V
AV
Uporov
E V
EV
Tsyrlina
K M
KM
Pozharisskiĭ
D001941:Breast Diseases / Q000473:pathology; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology* / Q000503:physiopathology; D018270:Carcinoma, Ductal, Breast / Q000473:pathology; D002455:Cell Division; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D019394:Ki-67 Antigen / Q000378:metabolism*; D009697:Nucleolus Organizer Region
D019394:Ki-67 Antigen