Regulation of fibroblast-induced collagen gel contraction by interleukin-1beta.
Fibroblasts incorporated within collagen gels induce a cell-mediated contraction of the gel to form a three-dimensional, tissue-like structure by a mechanism thought to mimic wound contraction in vivo. In this study a gel contraction model was used to investigate the ability of fibroblasts derived from adult gingiva, adult skin and fetal skin to organise a collagen matrix. In addition the effects of interleukin-1beta (IL-1beta) on the contraction process was also investigated. Over the concentration range 5-50 U/ml, IL-1beta induced a statistically significant inhibition of gel contraction in all fibroblast cell types (P<0.05), although fetal fibroblasts appeared least responsive and gingival fibroblasts most responsive to the inhibitory effects of this cytokine. Comparison of gel contraction by the different fibroblast strains indicated that fetal and gingival fibroblasts shared similar contraction kinetics. For the adult skin fibroblasts, three of five strains studied showed significantly diminished levels of gel contraction compared to fetal and gingival cells. This apparent difference in fibroblast phenotype may, at least in part, explain the fetal-like wound healing pattern seen in the oral mucosa.
Department of Restorative Dentistry, Queen's University, Belfast, N. Ireland.
C R
CR
Irwin
T
T
Myrillas
M
M
Smyth
J
J
Doogan
C
C
Rice
S L
SL
Schor
D000328:Adult; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D005333:Fetus; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D005782:Gels; D005881:Gingiva / Q000166:cytology; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000008:administration & dosage / Q000494:pharmacology*; D010641:Phenotype; D012867:Skin / Q000166:cytology / Q000196:embryology; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014945:Wound Healing / Q000502:physiology
D016861:Cyclooxygenase Inhibitors; D005782:Gels; D007375:Interleukin-1; D016212:Transforming Growth Factor beta; D003094:Collagen; D007213:Indomethacin