Comet assay on children's leukocytes 8 years after the Chernobyl disaster.
DNA damage, mainly single strand breaks, was evaluated by single cell gel electrophoresis, in leukocytes of 36 healthy and 14 thyroid cancer-affected children prior to radio-therapy. The children come from the Gomel region, one of the areas most heavily radio-contaminated by the Chernobyl fallout. In addition, leukocytes were treated with a challenge dose of bleomycin (BLM, 1.5 micrograms/ml), to assess the presence of an adaptive response (AR) potentially resulting from chronic exposure to radionuclides. As controls, 13 children living in Pisa (Italy) were enrolled in the study. Children with thyroid cancer show higher (p < 0.001) DNA damage than healthy ones. No difference was found between healthy children from Gomel and from Pisa. A reduction in the response to BLM was significantly linked to low plasma levels of FT4 hormone (p < 0.0001), to the presence of the tumor (p < 0.002), to being female (p < 0.02), and to a higher 137Cs body burden (p < 0.03).
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università di Pisa, Italy.
G
G
Frenzilli
A
A
Lori
G
G
Panasiuk
M
M
Ferdeghini
R
R
Barale
D000293:Adolescent; D001681:Biological Assay; D001761:Bleomycin / Q000008:administration & dosage; D002648:Child; D004249:DNA Damage; D004190:Disasters; D005260:Female; D006801:Humans; D007962:Leukocytes / Q000528:radiation effects*; D008297:Male; D011210:Power Plants; D018788:Radioactive Hazard Release; D013964:Thyroid Neoplasms / Q000235:genetics*; D014455:Ukraine
D001761:Bleomycin