Protective effect of vaccination with a combination of recombinant surface antigen 1 and interleukin-12 against toxoplasmosis in mice.
We studied the immune response induced in mice by recombinant Toxoplasma gondii surface antigen 1 (rSAG1) protein, alone or combined with interleukin-12 (IL-12) as an adjuvant, and the protective effect against toxoplasmosis. Immunization with rSAG1 alone induced a specific humoral type 2 immunity and did not protect the animals from infection. In contrast, immunization with rSAG1 plus IL-12 redirected humoral and cellular immunity toward a type 1 pattern and reduced the brain parasite load by 40%.
Institut de Parasitologie et de Pathologie Tropicale, Inserm U392, Faculté de Médecine, 67000 Strasbourg, France.
V
V
Letscher-Bru
O
O
Villard
B
B
Risse
M
M
Zauke
J P
JP
Klein
T T
TT
Kien
D000276:Adjuvants, Immunologic; D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000276:immunology; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D001921:Brain / Q000469:parasitology / Q000473:pathology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D007376:Interleukin-2 / Q000235:genetics / Q000627:therapeutic use*; D051379:Mice; D008808:Mice, Inbred CBA; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D014122:Toxoplasma / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000517:prevention & control; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016207:Cytokines; D007376:Interleukin-2; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma; D014614:Vaccines, Synthetic