Characterization of the BcLF1 promoter in Epstein-Barr virus.
The location of the BcLF1 promoter of Epstein-Barr virus (EBV) has been identified by primer extension, which indicates that the +1 site of the BcLF1 mRNA is located at nucleotide 137676 of the EBV genome. According to deletion analysis, the region upstream from nucleotide -38 is not essential for transcription of BcLF1. A 23 bp region in the promoter, from nucleotide -38 to -16, was identified as necessary for regulating the expression of BcLF1, i.e. the promoter activity is activated by 12-O-tetradecanoylphorbol 13-acetate but is repressed by phosphonoacetic acid. The results presented also demonstrate that the oriLyt sequence in cis is essential for enhancing the expression of BcLF1.
Institute of Microbiology and Immunology, National Yang-Ming University, Shih-Pai, Taipei, Taiwan.
P J
PJ
Chang
Y S
YS
Chang
S T
ST
Liu
D002213:Capsid / Q000235:genetics*; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic