Antigenic definition of cardiac troponin I.
The presence of cardiac troponin I in the serum is now considered as one of the most specific biochemical markers of acute myocardial infarction. To improve the knowledge of the antigenic properties of cardiac Troponin I, a set of monoclonal antibodies and polyclonal antibodies against human cardiac troponin I has been tested with overlapping peptides covering the cardiac troponin I sequence. The results indicate that N-terminal and C-terminal cardiac troponin I regions were most often recognized by poly- and monoclonal antibodies. These observations are valuable for choosing the best combination of monoclonal antibodies to set up new immunoassays to detect serum cardiac troponin I earlier after myocardial damage, to understand better which forms are released, and finally to propose appropriate cardiac troponin I standards.
Larue
C
C
Sanofi Diagnostics Pateur, Marnes-la-Coquette, France. catherine.larue@sanofi.com
Ferrieres
G
G
Laprade
M
M
Calzolari
C
C
Granier
C
C
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D015415:Biomarkers; D018604:Epitope Mapping; D006801:Humans; D007536:Isomerism; D009203:Myocardial Infarction / Q000175:diagnosis / Q000503:physiopathology; D009206:Myocardium / Q000276:immunology*; D012680:Sensitivity and Specificity; D019210:Troponin I / Q000097:blood / Q000737:chemistry / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000941:Antigens; D015415:Biomarkers; D019210:Troponin I