Gene arrangement of the killer cell inhibitory receptor family on human chromosome 19q13.4 detected by fiber-FISH.
Human chromosome 19q13.4 has recently been revealed to be a remarkable region harboring multiple receptor genes of the immunoglobulin (Ig) superfamily differentially expressed on hematopoietic cell lineages. Over the past few years, more than 50 cDNAs have been cloned for the natural killer cell inhibitory receptor (KIR) gene family, which possess two or three Ig-like domains in the extracellular region. In this study, using two genomic DNA probes containing intron sequences of genes corresponding to the two- and three-domain types, we applied two-color-fluorescence in situ hybridization on stretched DNA fiber preparations (fiber-FISH). As a result, 11 positions homologous to KIR genes were found as a cluster within a range of approximately 120 kilobases on a chromatin fiber from human chromosome 19.
Department of Research, The Japanese Red Cross Central Blood Center, 1-31, Hiroo 4-chome, Shibuya-ku, Tokyo 150, Japan.
Y
Y
Suto
Y
Y
Ishikawa
M
M
Kasahara
F
F
Kasai
T
T
Yabe
T
T
Akaza
T
T
Juji
D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D015342:DNA Probes; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007694:Killer Cells, Natural; D005810:Multigene Family; D011971:Receptors, Immunologic / Q000235:genetics*; D054340:Receptors, KIR
D015342:DNA Probes; D011971:Receptors, Immunologic; D054340:Receptors, KIR