Detection of Newcastle disease virus-specific antibodies in ostrich sera by three serological methods.
Sera from 211 ostriches were tested for the presence of Newcastle disease virus (NDV)-specific antibodies by the virus neutralisation test, the haemagglutination inhibition (HI) test and a recently developed avian paramyxovirus serotype 1 (PMV-1) specific monoclonal antibody blocking ELISA (b-ELISA). The virus neutralisation test was used as the reference for the estimation of the sensitivity and specificity of the b-ELISA and HI tests. Of the 211 sera, 140 contained NDV-specific neutralising antibodies, 130 were positive by the HI test and 122 by the b-ELISA. The sensitivity, specificity and predictive accuracy of the HI and b-ELISA tests relative to the virus neutralisation test were similar. The good agreement between the HI and b-ELISA test (kappa = 0.85) suggested that the two methods are interchangeable.
Institute of Animal Science and Health, Department of Avian Virology, Lelystad, The Netherlands.
G
G
Koch
G
G
Czifra
B E
BE
Engström
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000032:analysis*; D001717:Birds / Q000821:virology*; D004797:Enzyme-Linked Immunosorbent Assay; D006386:Hemagglutination Tests; D009500:Neutralization Tests; D009521:Newcastle Disease / Q000175:diagnosis* / Q000276:immunology; D019213:Rubulavirus / Q000276:immunology; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral