The isolation of genomic DNA from blackcurrant (Ribes nigrum L.).
A method is described for isolating DNA of high molecular mass (M(r)) from blackcurrant and other softfruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.
Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland, UK. mwoodh@scri.sari.ac.uk
M
M
Woodhead
H V
HV
Davies
R M
RM
Brennan
M A
MA
Taylor
D018744:DNA, Plant / Q000302:isolation & purification*; D005638:Fruit / Q000235:genetics*; D018745:Genome, Plant
D018744:DNA, Plant