Lymphocytic choriomeningitis virus-induced alterations of T helper-mediated responses in mice developing autoimmune hemolytic anemia during the course of infection.
The effect of LCMV on CD4+ T lymphocytes was analyzed in C3HeB/FeJ mice after infection with the Docile strain of this virus. Our results indicated that LCMV triggers: i) an inhibition of Th2 lymphocyte differentiation induced by concomitant immunization with a nonviral protein antigen; ii) a depression of T helper-dependent antibody responses elicited by such an immunization; and iii) a CD4+ cell-mediated proliferation of spleen cells leading to increased interleukin-4 and interferon-gamma message expression and IgG2a-restricted total immunoglobulin secretion. Taken together, these results indicate that LCMV profoundly affects CD4+ cell-mediated immune responses in infected animals. Such modulations of T-helper functions may explain the preponderance of IgG2a in the antierythrocyte autoimmune response induced by the virus in C3HeB/FeJ mice.
El Azami El Idrissi
M
M
Unit of Experimental Medicine, International Institute for Cellular Pathology, Université Catholique de Louvain, Bruxelles, Belgium.
Mazza
G
G
Monteyne
P
P
Elson
C J
CJ
Day
M J
MJ
Pfau
C J
CJ
Coutelier
J P
JP
D000743:Anemia, Hemolytic / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001327:Autoimmune Diseases / Q000276:immunology* / Q000821:virology; D002460:Cell Line; D004285:Dogs; D005260:Female; D006433:Hemocyanins / Q000008:administration & dosage / Q000276:immunology; D007114:Immunization; D007668:Kidney; D008213:Lymphocyte Activation; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D008974:Mollusca / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology* / Q000821:virology
D000941:Antigens; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin