Oncogene amplification and expression in pediatric solid tumors.
Oncogene amplification and expression and their mutual relationship was analyzed in 92 pediatric tumors by Southern and Northern blot hybridization with N-MYC, ERB A, ERB B, N-RAS and Shb probes. Amplification and overexpression was associated with more advanced clinical stages of tumor, especially in neuroblastomas, rhabdomyosarcomas and ganglioneuroblastomas. The most frequent alteration observed was N-MYC amplification together with overexpression. N-RAS amplification was not detected, while the overexpression of this oncogene was found in 3 cases. Neither amplification nor overexpression was revealed in any specimen of hepatoblastoma or hepatocellular carcinoma. We suggest that oncogenes overexpression provides more accurate prognostic information than amplification.
Mares
J
J
Institute of Biology and Medical Genetics, 2nd Medical School, Charles' University, Prague, Czech Republic.
Polanská
V
V
Görgens
H
H
Sedlácek
Z
Z
Maríková
T
T
Bocek
P
P
Kodet
R
R
Schackert
J
J
Goetz
P
P
D002648:Child; D002675:Child, Preschool; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D009857:Oncogenes; D016133:Polymerase Chain Reaction