Differential effect of genistein on transforming growth factor beta 1 expression in normal and malignant mammary epithelial cells.
Our present study demonstrates that the isoflavone genistein exerts a differential response in normal and malignant breast epithelial cells. The addition of genistein results in a dose-dependent increase in TGF-beta 1 mRNA expression in normal human mammary epithelial cells (HMEC) but not in tumor-derived MCF-7 cells. Genistein inhibits the growth and also causes an increase in apoptosis of HMEC. The increased expression of TGF-beta 1 may contribute to the growth inhibitory as well as apoptotic effects of genistein on HMEC.
Laboratory of Nutritional and Molecular Regulation, NCI-Frederick Cancer Research and Development Center, NIH, MD 21702-1201, USA.
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N
Sathyamoorthy
J S
JS
Gilsdorf
T T
TT
Wang
D017209:Apoptosis / Q000187:drug effects; D001940:Breast / Q000166:cytology / Q000378:metabolism*; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics; D012333:RNA, Messenger / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D019833:Genistein