[PCR analysis of microsatellite DNA markers: possibility of erroneous determination of genotypes].
A comparative study of two techniques for the PCR genotyping of highly polymorphic tandem repeats was carried out by the example of a triplet repeat in the myotonin protein kinase gene. Sequencing denaturing gels were shown to yield more precise results in the analysis of amplification products.
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
S N
SN
Popova
M I
MI
Shadrina
R I
RI
Fatkhlislamova
E K
EK
Khusnutdinova
P A
PA
Slominskiĭ
D004276:DNA, Satellite / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D005838:Genotype; D065636:Myotonin-Protein Kinase; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D005819:Genetic Markers; D011494:Protein Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases