Organization of the equine immunoglobulin heavy chain constant region genes; III. Alignment of c mu, c gamma, c epsilon and c alpha genes.
Previous restriction analysis of cloned equine DNA and genomic DNA of equine peripheral blood mononuclear cells had indicated the existence of one c epsilon, one c alpha and up to six c gamma genes in the haploid equine genome. The c epsilon and c alpha genes have been aligned on a 30 kb DNA fragment in the order 5' c epsilon-c alpha 3'. Here we describe the alignment of the equine c mu and c gamma genes by deletion analysis of one IgM, four IgG and two equine light chain expressing heterohybridomas. This analysis establishes the existence of six c gamma genes per haploid genome. The genomic alignment of the cH-genes is 5' c mu/(/) c gamma 1/(/) c gamma 2/(/) c gamma 3/(/) c gamma 4/(/) c gamma 5/(/) c gamma 6/(/) c epsilon-c alpha 3', naming the c gamma genes according to their position relative to c mu. For three of the c gamma genes the corresponding IgG isotypes could be identified as IgGa for c gamma 1, IgG(T) for c gamma 3 and IgGb for c gamma 4.
Immunology Unit, Hannover School of Veterinary Medicine, Germany. bwagner@immunologie.tiho-hannover.de
B
B
Wagner
G
G
Overesch
A S
AS
Sheoran
M A
MA
Holmes
C
C
Richards
W
W
Leibold
A
A
Radbruch
D000818:Animals; D001426:Bacterial Proteins; D015342:DNA Probes; D015245:Deoxyribonuclease BamHI; D015252:Deoxyribonucleases, Type II Site-Specific; D017353:Gene Deletion; D005803:Genes, Immunoglobulin; D006736:Horses / Q000235:genetics / Q000276:immunology*; D006825:Hybridomas; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007134:Immunoglobulin Switch Region / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping
D001426:Bacterial Proteins; D015342:DNA Probes; D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D015245:Deoxyribonuclease BamHI; C024404:BglII endonuclease; D015252:Deoxyribonucleases, Type II Site-Specific