Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.
A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI, shares 66% identity and 76% similarity with M. Cvi JI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.
Department of Biochemistry and Molecular Biology and the Center for Gene Regulation, 308 Althouse Laboratory,The Pennsylvania State University, University Park, PA 16802-4500, USA.
M
M
Xu
M P
MP
Kladde
J L
JL
Van Etten
R T
RT
Simpson
D000595:Amino Acid Sequence; D002708:Chlorella / Q000821:virology*; D002843:Chromatin / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D015698:Genomic Library; D008745:Methylation; D008969:Molecular Sequence Data; D017862:Phycodnaviridae / Q000201:enzymology / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D002843:Chromatin; D017931:DNA Primers; D015226:Dinucleoside Phosphates; C054544:2'-deoxyguanylyl-(3'-5')-2'-deoxycytidine; D004248:DNA (Cytosine-5-)-Methyltransferases