Role of CR4 in Mycobacterium tuberculosis-human macrophages binding and signal transduction in the absence of serum.
The beta2 integrin CR4 is involved in Mycobacterium tuberculosis phagocytosis by human mononuclear phagocytes through the opsonin C3bi. In this study, we demonstrate that M. tuberculosis can bind directly to monocyte-derived macrophages via CR4 in the absence of any opsonins. CR4-transfected CHO cells gave similar results, suggesting recognition by CR4 of bacterial structure. Furthermore, binding of M. tuberculosis transduced a potent signal, resulting in tyrosine phosphorylation of macrophage proteins, which was in part mediated by CR4.
Department of Medicine, Case Western Reserve University, and University Hospitals, Cleveland, Ohio 44106-4984, USA. zaffray@cesmtp.ccf.org
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Zaffran
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Zhang
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Ellner
D000818:Animals; D016466:CHO Cells; D002478:Cells, Cultured; D006224:Cricetinae; D006801:Humans; D016167:Integrin alphaXbeta2 / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction
D016167:Integrin alphaXbeta2