A human RNA polymerase II complex containing factors that modify chromatin structure.
We have isolated a human RNA polymerase II complex that contains chromatin structure remodeling activity and histone acetyltransferase activity. This complex contains the Srb proteins, the Swi-Snf complex, and the histone acetyltransferases CBP and PCAF in addition to RNA polymerase II. Notably, the general transcription factors are absent from this complex. The complex was purified by two different methods: conventional chromatography and affinity chromatography using antibodies directed against CDK8, the human homolog of the yeast Srb10 protein. Protein interaction studies demonstrate a direct interaction between RNA polymerase II and the histone acetyltransferases p300 and PCAF. Importantly, p300 interacts specifically with the nonphosphorylated, initiation-competent form of RNA polymerase II. In contrast, PCAF interacts with the elongation-competent, phosphorylated form of RNA polymerase II.
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Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.
Orphanides
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Sun
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Yang
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XJ
Ogryzko
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Lees
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Nakatani
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Reinberg
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D
D000123:Acetyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000302:isolation & purification / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism* / Q000648:ultrastructure; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases / Q000302:isolation & purification / Q000378:metabolism; D006367:HeLa Cells; D051548:Histone Acetyltransferases; D006801:Humans; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000302:isolation & purification / Q000378:metabolism; D012319:RNA Polymerase II / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors / Q000032:analysis; D014158:Transcription, Genetic; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor
D000123:Acetyltransferases; D018797:Cell Cycle Proteins; D002843:Chromatin; D056850:Cyclin-Dependent Kinase 8; D018844:Cyclin-Dependent Kinases; D051548:Histone Acetyltransferases; D017346:Protein Serine-Threonine Kinases; D012319:RNA Polymerase II; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D050880:p300-CBP Transcription Factors; D000099224:p300-CBP-Associated Factor; C095686:CDK8 protein, human; C498309:SSN3 protein, S cerevisiae