Characterization of an ammonium transport protein from the peribacteroid membrane of soybean nodules.
Nitrogen-fixing bacteroids in legume root nodules are surrounded by the plant-derived peribacteroid membrane, which controls nutrient transfer between the symbionts. A nodule complementary DNA (GmSAT1) encoding an ammonium transporter has been isolated from soybean. GmSAT1 is preferentially transcribed in nodules and immunoblotting indicates that GmSAT1 is located on the peribacteroid membrane. [14C]methylammonium uptake and patch-clamp analysis of yeast expressing GmSAT1 demonstrated that it shares properties with a soybean peribacteroid membrane NH4<SUP ARRANGE="STAGGER">+ channel described elsewhere. GmSAT1 is likely to be involved in the transfer of fixed nitrogen from the bacteroid to the host.
Division of Biochemistry and Molecular Biology, The Australian National University, Canberra ACT 0200, Australia.
B N
BN
Kaiser
P M
PM
Finnegan
S D
SD
Tyerman
L F
LF
Whitehead
F J
FJ
Bergersen
D A
DA
Day
M K
MK
Udvardi
D000595:Amino Acid Sequence; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D027682:Cation Transport Proteins; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary; D007473:Ion Channels / Q000378:metabolism; D007700:Kinetics; D008744:Methylamines / Q000378:metabolism; D008969:Molecular Sequence Data; D015388:Organelles / Q000378:metabolism; D018408:Patch-Clamp Techniques; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D011188:Potassium / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D030262:Soybean Proteins; D013025:Glycine max / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D013104:Spheroplasts / Q000378:metabolism; D013559:Symbiosis; D014170:Transformation, Genetic
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D007473:Ion Channels; D008744:Methylamines; D000644:Quaternary Ammonium Compounds; C114002:SAT1 protein, glycine max; D030262:Soybean Proteins; C027451:methylamine; D011188:Potassium