Decolourization and biodegradation of N,N'-dimethyl-p-phenylenediamine by Klebsiella pneumoniae RS-13 and Acetobacter liquefaciens S-1.
Klebsiella pneumoniae RS-13 and Acetobacter liquefaciens S-1, both methyl red (MR)-degrading bacterial strains, degraded N,N'-dimethyl-p-phenylenediamine (DMPD) under aerobic conditions. DMPD, a toxic and mutagenic aromatic amine, is formed during the reductive cleavage of azo dyes such as MR. The effects of physical parameters, such as temperature and aeration, and chemical parameters, such as pH and concentrations of glucose, ethanol and ammonium sulphate in the culture medium, on the degradation of DMPD by these bacteria were determined. Klebsiella pneumoniae RS-13 degraded DMPD more efficiently than A. liquefaciens S-1 under all physicochemical conditions, except in the presence of ethanol as carbon and energy sources. In addition, Kl. pneumoniae RS-13 degraded DMPD at low levels of carbon and nitrogen at pH 6-8. These results indicate that it is feasible to use Kl. pneumoniae RS-13 to completely degrade the detoxify MR under aerobic conditions.
Department of Biology, Chinese University of Hong Kong, China.
P K
PK
Wong
P Y
PY
Yuen
D000091:Acetobacter / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000378:metabolism / Q000494:pharmacology; D001391:Azo Compounds / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004396:Coloring Agents / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism; D010655:Phenylenediamines / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D001391:Azo Compounds; D004396:Coloring Agents; D005456:Fluorescent Dyes; D010655:Phenylenediamines; D000431:Ethanol; C008492:methyl red; C004781:dimethyl-4-phenylenediamine; D005947:Glucose; D010100:Oxygen; D000645:Ammonium Sulfate