Alterations in early biochemical events following T cell activation in leprosy patients.
The early events of activation and cytokine profiles (IL-2, 4, and 6) were studied in lymphocytes of paucibacillary (TT/BT) and multibacillary (BL/LL) leprosy patients after stimulation with PMA/A23187 and Mycobacterium leprae antigen (PGL-1). Lymphocytes from BT/TT patients showed proliferation in response to both PMA/A23187 and PGL-1 compared to BL/LL. The levels of early activation signaling molecules such as IP3, calcium, and protein kinase C (PKC) in the particulate fraction were found to be elevated in BT/TT and BL/LL patients and showed a further significant increase after stimulation with PMA/A23187 in BT/TT patients. PGL-1 marginally increased the IP3 levels in BT/TT patients, whereas in BL/LL patients, it had no effect. The levels of IL-2 were enhanced in lymphocytes of BT/TT leprosy patients and were further augmented by PPD and PGL-1, while the levels of IL-4 and IL-6 were increased in LL/BL lymphocytes and further augmented by PGL-1. Thus PGL-1 seems to be a major culprit in inducing the TH2-type cytokine response observed in lepromatous leprosy patients.
Department of Dermatology, Venerology, and Leprology, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India.
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Sharma
V K
VK
Sharma
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A
Gupta
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Kaur
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Kaur
N K
NK
Ganguly
D000942:Antigens, Bacterial; D015703:Antigens, CD / Q000032:analysis; D002118:Calcium / Q000032:analysis; D002462:Cell Membrane / Q000737:chemistry; D002478:Cells, Cultured; D006017:Glycolipids / Q000494:pharmacology; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007376:Interleukin-2 / Q000032:analysis; D015847:Interleukin-4 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007424:Intracellular Fluid / Q000737:chemistry; D015440:Leprosy, Lepromatous / Q000276:immunology*; D015441:Leprosy, Tuberculoid / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects / Q000502:physiology; D011493:Protein Kinase C / Q000187:drug effects / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000942:Antigens, Bacterial; D015703:Antigens, CD; D006017:Glycolipids; D007295:Inositol Phosphates; D007376:Interleukin-2; D015850:Interleukin-6; C045169:phenolic glycolipid I, Mycobacterium leprae; D015847:Interleukin-4; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium