Co-localization of estrogen receptor and aromatase enzyme immunoreactivities in adult musk shrew brain.
Estrogens are produced by the aromatization of androgens. These steroids exert their actions after binding to their receptors. Past studies have shown that estrogen receptors (ER) and aromatase enzyme (AROM) reside in many of the same brain regions. Few studies, however, have examined the neural co-localization of these important components involved in estrogen-activated behaviors. In the present study we examined the co-localization of ER and AROM immunoreactive (ir) neurons in musk shrew (Suncus murinus) brains. Data were collected from a representative section from three neural regions, the bed nucleus of the stria terminalis (BNST), medial preoptic area (mPOA), and ventromedial nucleus of the hypothalamus (VMN). Here we report a sex difference in the number of ER-ir neurons from the analyzed section of the mPOA and BNST. Females have more ER-ir neurons in the mPOA and males have more in the BNST. In the sections we examined, males tended to have more aromatase containing neurons than females. Although there were no significant differences in the numbers of double-labeled cells, the VMN contains the greatest percentage of these cells in both males and females; followed by the mPOA and the BNST. In addition, in the mPOA of both sexes, a distinct nucleus of aromatase containing neurons which was devoid of ER immunoreactivity was noted. Area measurements of the AROM-ir nucleus showed that it was significantly larger in males than in females. Taken together, these data suggest that there is not extensive cellular co-localization of estrogen receptors and aromatase enzyme in the musk shrew brain. However, the presence of other genomic forms of ER (membrane and/or ERbeta) in AROM containing neurons has not been ruled out by this study. Thus, we hypothesize that estrogens produced in brain affect behavior by binding to ER in neurons other than those that contain aromatase enzyme.
Veney
S L
SL
Department of Biology, University of Virginia, Charlottesville, Virginia, 22903, USA.
Rissman
E F
EF
D000367:Age Factors; D000818:Animals; D001141:Aromatase / Q000032:analysis*; D001923:Brain Chemistry / Q000502:physiology*; D005260:Female; D008297:Male; D011301:Preoptic Area / Q000737:chemistry / Q000201:enzymology; D011960:Receptors, Estrogen / Q000032:analysis*; D012686:Septal Nuclei / Q000737:chemistry / Q000201:enzymology; D012727:Sex Characteristics; D012788:Shrews / Q000502:physiology*; D014697:Ventromedial Hypothalamic Nucleus / Q000737:chemistry / Q000201:enzymology
D011960:Receptors, Estrogen; D001141:Aromatase