Zymodeme and serodeme characterization of Leishmania isolates obtained from Costa Rican patients.
Thirty-four Leishmania isolates obtained from Costa Rican patients, from different geographical areas, were characterized by isoenzyme electrophoresis and indirect immunofluorescense with monoclonal antibodies. Thirty-two were characterized as L. panamensis strains and two were L. braziliensis variants. We confirm the evident predominance of L. panamensis as the main etiological agent of leishmaniasis in Costa Rica and the existence of L. braziliensis in the country.
Peraza
J
J
Laboratorio de Zoonosis, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.
Urbina
A
A
Zeledón
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002648:Child; D003364:Costa Rica; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007223:Infant; D007527:Isoenzymes; D007891:Leishmania / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification*; D007892:Leishmania braziliensis / Q000201:enzymology / Q000302:isolation & purification; D018334:Leishmania guyanensis / Q000201:enzymology / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D007527:Isoenzymes