High-performance liquid chromatographic assay for a new fasciolicide agent, alphaBIOF10, in biological fluids.
This paper describes a high-performance liquid chromatographic method with ultraviolet absorbance detection at 304 nm for the determination of 6-chloro-5-(1-naphthyloxy)-2-methylthio benzimidazole (alphaBIOF10) -- a new fasciolicide agent -- and its sulphoxide (SOalphaBIOF10), in plasma and urine. It requires 2 ml of biological fluid, an extraction using Sep-Pak cartridges, and methanol for drug elution. Analysis is performed on a microBondapak C18 (10 microm) column, using methanol-acetonitrile-water (40:30:30, v/v) as the mobile phase. Results showed that the assay is sensitive: 7.2 ng/ml for alphaBIOF10 and SOalphaBIOF10 in plasma and 3.6 ng/ml for both compounds in urine. The response was linear between 0.195 and 12.5 microg/ml. Maximum intra-day coefficient of variation was 5.3%. Recovery obtained was 97.8% for both alphaBIOF10 and SOalphaBIOF10. In urine, recovery was 99.6% and 93.1% for alphaBIOF10 and SOalphaBIOF10 respectively. The method was used to perform a preliminary pharmacokinetic study in two sheep and was found to be satisfactory.
Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, México DF.
L M
LM
del Rivero
H
H
Jung
R
R
Castillo
A
A
Hernández-Campos
D000818:Animals; D000980:Antiplatyhelmintic Agents / Q000097:blood* / Q000652:urine*; D001562:Benzimidazoles / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D009281:Naphthalenes / Q000097:blood* / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep
D000980:Antiplatyhelmintic Agents; D001562:Benzimidazoles; D009281:Naphthalenes; C114686:alphaBIOF10