Identification and mapping of 26 human testis mRNAs containing CAG/CTG repeats.
Various types of pathologies, including neurodegenerative diseases, as well as different types of neoplasia, are related to genes exhibiting simple tandem repeat instabilities. In order to seek for new candidate genes for such disorders, we screened 4.10(6) human testis cDNAs for CAG- and CTG-containing clones. Among 910 positive clones, we characterized 109 cDNAs corresponding to 26 independent mRNAs. Fourteen of these mRNAs represent new genes. The corresponding clones contain between 3 and 19 consecutive CAG or CTG triplets. We assigned 15 out of these 26 genes to 14 different human chromosomes. These genes represent new potential candidates for diseases associated with CAG or CTG repeat mutations.
Institut National de la Santé et de la Recherche Médicale (INSERM), Unité 99, Hôpital Henri Mondor, 94010 Créteil, France.
A
A
Pawlak
N
N
Chiannikulchai
W
W
Ansorge
F
F
Bulle
J
J
Weissenbach
G
G
Gyapay
G
G
Guellaën
D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis; D005796:Genes; D005820:Genetic Testing; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D013737:Testis / Q000737:chemistry*; D018911:Trinucleotide Repeats / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger