Regional differences in the effects of glutamate uptake inhibitor L-trans-pyrrolidine-2,4-dicarboxylic acid on extracellular amino acids and dopamine in rat brain: an in vivo microdialysis study.
The effects of the glutamate transporter inhibitor, trans-PDC, on extracellular amino acids, were investigated in the frontal cortex, striatum, hippocampus and cerebellum of rats using in vivo microdialysis. Trans-PDC infusion (0.1, 1, 10 mM) dose-dependently increased Glu and Asp levels, and these increases in the cerebellum were smaller than those in other brain regions. A small but significant dose-dependent increase was observed for Gly and Tau. However, high extracellular Glu induced by trans-PDC was not sufficient to increase extracellular DA in the striatum and frontal cortex.
Division of Health Sciences, University of the Air, Chiba, Japan. sembaj@u-air.ac.jp
J
J
Semba
M S
MS
Wakuta
D018528:ATP-Binding Cassette Transporters / Q000037:antagonists & inhibitors / Q000378:metabolism; D027322:Amino Acid Transport System X-AG; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D014179:Neurotransmitter Uptake Inhibitors / Q000494:pharmacology*; D017397:Prefrontal Cortex / Q000187:drug effects / Q000378:metabolism; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism; D017557:Ventral Tegmental Area / Q000187:drug effects / Q000378:metabolism; D014793:Visual Cortex / Q000187:drug effects / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D000596:Amino Acids; D003998:Dicarboxylic Acids; D014179:Neurotransmitter Uptake Inhibitors; D011759:Pyrrolidines; D018698:Glutamic Acid; C083486:pyrrolidine-2,4-dicarboxylic acid; D004298:Dopamine