Absorption and induction of micronucleated peripheral reticulocytes in mice after oral administration of fragrant hydroxyfuranones generated in the Maillard reaction.
Fragrant hydroxyfuranone and dihydroxypyranone derivatives generated in the Maillard reaction of sugars and amino acids are detected in various processed foods and have been shown active to break DNA single-strand in the in vitro studies. In the present study, absorption of 2,5-dimethyl-4-hydroxy-3(2 H)-furanone (DMHF) and 4-hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2 H)-furanone (HEMF), both found in soy sauce, into plasma after a single intraperitoneal or oral administration at doses of 0.5-1.0 gkg-1 to mice was examined. Both compounds appeared in plasma 15 min after intraperitoneal administration and disappeared 2 h after the administration. They appeared in plasma 5 min after oral administration, reached maximum after 15-45 min, and gradually disappeared after 2 h, indicating that they are absorbed by the digestive tract. Both DMHF and HEMF induced micronucleated reticulocytes (MNRETs) in mouse peripheral blood in a dose-dependent manner after oral administration. The results indicate that DMHF and HEMF can cause genetic damage after oral administration.
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan.
K
K
Hiramoto
T
T
Kato
Y
Y
Takahashi
K
K
Yugi
K
K
Kikugawa
D000042:Absorption; D000284:Administration, Oral; D000818:Animals; D005663:Furans / Q000008:administration & dosage / Q000097:blood / Q000493:pharmacokinetics*; D015416:Maillard Reaction; D051379:Mice; D015162:Micronucleus Tests; D015394:Molecular Structure; D012156:Reticulocytes / Q000187:drug effects*
D005663:Furans; C058427:furaneol; C112945:4-hydroxy-2-ethyl-5-methyl-3(2H)-furanone