Augmentation of IgM antibody to gp43 tumor-associated antigen peptide by melanoma cell vaccine.
We previously reported that gp43 tumor-associated antigen peptide (DLTMKYQIF; designated 810 antigen) on human melanoma cells is recognized by IgM human monoclonal antibody L92 and by cytotoxic T lymphocytes (CTL). In this study, we retrospectively tested sera of 44 patients with regional metastatic melanoma (22 who recurred within 1 year and 22 who survived longer than 5 years) to determine if antibody responses to 810 antigen could be induced by immunization with an allogeneic melanoma cell vaccine that contained 810 peptide. IgM and IgG antibodies were assessed by enzyme-linked immunosorbent assay using a synthetic 810 nonamer peptide. A significant augmentation of IgM antibody was demonstrated 4 weeks after initiation of vaccine therapy, and the IgM level was significantly higher in patients who survived more than 5 years. The antigen epitope recognized by antibodies was located within TMKYQI. Of this epitope sequence, K appears to play a central role in antigenicity. The 810 antigen recognized by antibody and CTL may have clinical relevance as a potential source of melanoma vaccine.
Department of Biotechnology Sciences, John Wayne Cancer Institute, Santa Monica, California.
T
T
Takahashi
A
A
Conforti
Y
Y
Kikumoto
D S
DS
Hoon
D L
DL
Morton
R F
RF
Irie
D000912:Antibodies, Neoplasm / Q000096:biosynthesis / Q000097:blood; D000951:Antigens, Neoplasm / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood*; D008545:Melanoma / Q000276:immunology* / Q000628:therapy*; D009362:Neoplasm Metastasis / Q000276:immunology; D009363:Neoplasm Proteins / Q000276:immunology; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D011379:Prognosis; D012189:Retrospective Studies; D012878:Skin Neoplasms / Q000276:immunology* / Q000628:therapy*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D007075:Immunoglobulin M; D009363:Neoplasm Proteins; D010455:Peptides; C107095:tumor-associated antigen, gp43