A protein kinase inhibitor from A431 subline overexpressing TGF alpha possesses antimicrobial activity.
During the last decade the key role of antimicrobial peptides in innate immunity has been argued. They were found in plants and in different phylogenic groups of animals (insects, amphibia, and even in mammals). We report the production of a human peptide antibiotic that was previously characterized as an EGF receptor tyrosine kinase inhibitor in epidermoid carcinoma A431/1522 cell subline overexpressing TGF alpha. It is a 3 kDa hydrophobic cationic peptide cytotoxic for different species of Gr+ and Gr- bacteria in micromolar concentration range, and demonstrating slight fungicidal activity.
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology of the National Academy of Science of Ukraine, Kiev, Ukraine.
P V
PV
Pogrebnoy
A I
AI
Hobta
M A
MA
Soldatkina
L V
LV
Garmanchouk
N V
NV
Markeeva
T
T
Eremenko
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell; D002460:Cell Line / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006090:Gram-Negative Bacteria / Q000187:drug effects*; D006094:Gram-Positive Bacteria / Q000187:drug effects*; D006801:Humans; D047428:Protein Kinase Inhibitors; D016211:Transforming Growth Factor alpha / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D047428:Protein Kinase Inhibitors; D016211:Transforming Growth Factor alpha