The Saccharomyces cerevisiae gene PSO5/RAD16 is involved in the regulation of DNA damage-inducible genes RNR2 and RNR3.
The expression of beta-galactosidase from DNA damage-inducible RNR2-lacZ and RNR3-lacZ fusion constructs was compared in wild-type (WT) and pso5/rad16 mutant strains after treatment with five mutagens/oxidative stressors. While exposure to the mutagens UVC, 4NQO and H2O2 induced expression of the RNR2-lacZ and RNR3-lacZ fusion constructs in two WT strains, treatment with the two oxidative stressors tBOOH and paraquat did not. In the pso5-1 mutant induction of RNR2-lacZ was largely reduced after UVC and H2O2 while there was no significant induction of beta-galactosidase expression after 4NQO treatment for this construct. For RNR3-lacZ there was strongly reduced expression of pso5-1 after UVC and 4NQO while H2O2 failed to induce expression of beta-galactosidase. In the WT strains the ranking of the inducing power of the mutagens at 90% survival (as measured in the pso5-1 mutant) was 4NQO>UVC>H2O2. Though the WT strains were clearly more resistant that the pso5-1 mutant to the two oxidative stressors paraquat and tBOOH, these substances failed to significantly enhance expression of the RNR2-lacZ and RNR3-lacZ fusion constructs in both the WT and the pso5-1 mutant. Our data suggest that Pso5p/Rad16p has a function in the signal transducing pathway controlling DNA damage-inducible components of nucleotide excision repair.
Paesi-Toresan
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SO
Centro de Biotecnologia and Depto. de Biofisica, UFRGS. Av. Bento Gonçalves, 9500, Campus do Vale, 91501-970 Porto Alegre - RS, Brazil. pegas@dna.cbiot.ufrgs.br
Maris
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Brendel
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Henriques
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D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000251:Adenosine Triphosphatases; D019677:Artificial Gene Fusion; D004249:DNA Damage / Q000235:genetics*; D004260:DNA Repair / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D005800:Genes, Fungal; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007763:Lac Operon; D009154:Mutation; D018384:Oxidative Stress / Q000235:genetics; D012264:Ribonucleotide Reductases / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D015112:4-Nitroquinoline-1-oxide; D006861:Hydrogen Peroxide; D012264:Ribonucleotide Reductases; D000251:Adenosine Triphosphatases; C075467:RAD16 protein, S cerevisiae