Construction and characterisation of a Yersinia enterocolitica O:8 ompR mutant.
The ompR-envZ two-component regulatory system has been shown to contribute to virulence in a number of enteric bacterial pathogens. A Yersinia enterocolitica O:8 ompR homologue was amplified, cloned and sequenced, showing 99.2% homology to the Escherichia coli OmpR. An isogenic ompR mutant was constructed by reverse genetics-based methodology. The mutant was shown to have increased sensitivity to high osmolarity, high temperature and low pH stresses in vitro. In the murine yersiniosis model, the mutant was attenuated and offered partial protection against wild-type challenge.
Department of Medical Microbiology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK.
N
N
Dorrell
S R
SR
Li
P H
PH
Everest
G
G
Dougan
B W
BW
Wren
D000818:Animals; D001426:Bacterial Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D005809:Genes, Regulator; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009154:Mutation; D009994:Osmolar Concentration; D016133:Polymerase Chain Reaction; D013696:Temperature; D015534:Trans-Activators / Q000235:genetics* / Q000302:isolation & purification; D014774:Virulence; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000472:pathogenicity
D001426:Bacterial Proteins; D015534:Trans-Activators; C112177:osmolarity response regulator proteins