On the concept of "normalized buffering power/conductivity ratio" of isoelectric buffers for capillary zone electrophoresis.
The introduction of a new physico-chemical parameter is proposed: the "normalized buffering power/conductivity ratio". It expresses the ratio of buffering power to conductivity (Rbeta/lambda), normalized by the electrolyte concentration, and gives an opportunity to calculate the properties of buffering ions typically used in capillary electrophoretic separations of biopolymers. This procedure of normalization is possible due to the fact that, in the concentration range practically used, the relationship of Rbeta/lambda on concentration is close to linear.
Institute of Gene Biology, Russian Academy of Sciences, Moscow.
A V
AV
Stoyanov
P G
PG
Righetti
D000665:Ampholyte Mixtures; D002021:Buffers; D004553:Electric Conductivity; D019075:Electrophoresis, Capillary; D007525:Isoelectric Focusing; D008432:Mathematical Computing
D000665:Ampholyte Mixtures; D002021:Buffers