Mapping of the natural resistance-associated macrophage protein 1 (NRAMP1) gene to pig chromosome 15.
The natural resistance-associated macrophage protein 1 (NRAMP1) was mapped in the pig for study as a potential candidate gene in controlling pig resistance to Salmonella infection. Primers were designed from the pig cDNA to amplify a 1.6 kb fragment between exons 1 and 3. By using a pig-rodent somatic cell hybrid panel, NRAMP1 was mapped to pig chromosome 15 (SSC15) with 100% probability, and the regional assignment was SSC15q23-26 with 87% concordance. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker was developed by using the HinfI enzyme and three alleles were identified from a population including 11 breeds. Linkage analysis confirmed the physical assignment by using the PiGMaP reference families. Pig NRAMP1 was linked to SSC15 markers S0088, S0149 and S0284 (LOD > 3). A small population study revealed large allele frequency differences among tested breeds. An A allele is only observed in dam (white) lines whereas a similar exclusivity of the C allele was seen in sire (colored) breeds.
Department of Animal Science, Iowa State University, Ames 50011, USA.
H S
HS
Sun
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Wang
M F
MF
Rothschild
C K
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Tuggle
D000483:Alleles; D000818:Animals; D000829:Animals, Domestic / Q000235:genetics*; D000835:Animals, Wild / Q000235:genetics*; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D027682:Cation Transport Proteins; D002874:Chromosome Mapping / Q000662:veterinary*; D017931:DNA Primers / Q000737:chemistry; D018076:DNA, Complementary / Q000737:chemistry; D005819:Genetic Markers; D014644:Genetic Variation / Q000235:genetics; D006822:Hybrid Cells / Q000737:chemistry; D007113:Immunity, Innate / Q000235:genetics; D008264:Macrophages / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011157:Population Dynamics; D015183:Restriction Mapping / Q000662:veterinary*; D012377:Rodentia / Q000235:genetics; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology; D013552:Swine / Q000235:genetics*; D013553:Swine Diseases / Q000235:genetics / Q000276:immunology; D000099173:Natural Resistance-associated Macrophage Protein 1
D002352:Carrier Proteins; D027682:Cation Transport Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D005819:Genetic Markers; D008565:Membrane Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1