Effect of substance P on the short-circuit current of rat nasal mucosal epithelium.
Rats were sensitized by intranasal application of toluene diisocyanate as a nasal allergy model. By means of the Using chamber technique, rat nasal epithelial short-circuit current (Isc) was measured. Enhanced Isc of the rat nasal mucosa resulted from stimulation of substance P (SP) in a dose-dependent manner that could be inhibited by pretreatment with NK1 receptor antagonist CP-96345, the H1 receptor antagonist pyrilamine, the H2 receptor antagonist ranitidine, and the neurotoxin tetrodotoxin, respectively, to different extents. The results indicate that SP is able to cause ion secretion of the nasal mucosal epithelium, perhaps by activating mast cells to release histamine. These data suggest that mast cells and sensory nerves participate in the regulation of SP-induced ion secretion during nasal allergy.
Department of Otolaryngology, First Affiliated Hospital, Shanxi Medical University, Taiyuan, People's Republic of China.
P C
PC
Yang
T
T
Liu
T Y
TY
Zhang
D S
DS
Fan
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology / Q000627:therapeutic use; D001713:Biphenyl Compounds / Q000494:pharmacology / Q000627:therapeutic use; D004195:Disease Models, Animal; D004558:Electric Stimulation / Q000379:methods; D004848:Epithelium / Q000187:drug effects; D006634:Histamine H1 Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D006635:Histamine H2 Antagonists / Q000494:pharmacology / Q000627:therapeutic use; D006636:Histamine Release / Q000187:drug effects; D008297:Male; D008407:Mast Cells / Q000187:drug effects; D009297:Nasal Mucosa / Q000187:drug effects / Q000378:metabolism*; D011738:Pyrilamine / Q000494:pharmacology / Q000627:therapeutic use; D011899:Ranitidine / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D006255:Rhinitis, Allergic, Seasonal / Q000188:drug therapy; D013373:Substance P / Q000187:drug effects* / Q000378:metabolism
D000894:Anti-Inflammatory Agents, Non-Steroidal; D001713:Biphenyl Compounds; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D013373:Substance P; D011899:Ranitidine; D011738:Pyrilamine; C066892:CP 96345