Use of a Hepta-tyr glycopeptide antibiotic as chiral selector in capillary electrophoresis.
A new glycopeptide antibiotic, MDL 63,246 (Hepta-tyr), of the teicoplanin family, has been evaluated in capillary electrophoresis for the resolution of chiral compounds of pharmaceutical and environmental interest. Electrophoretic separations were carried out in a polyacrylamide-coated capillary using the partial filling-counter current mode with aqueous-organic buffers in the pH range 4-6. Experimental parameters affecting resolution, such as antibiotic concentration, buffer pH, organic modifier type and capillary temperature, were studied. The Hepta-tyr antibiotic exhibited a high enantiorecognition capability towards the studied compounds at very low concentrations (1-2 mg/mL). The optimum experimental conditions were achieved by using a buffer at pH 5 containing acetonitrile at 25 degrees C.
Fanali
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Istituto di Cromatografia del C.N.R. Area della Ricerca di Roma, Monterotondo Scalo, Italy. fanali@nserv.icmat.mlib.cnr.it
Aturki
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Desiderio
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Bossi
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Righetti
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D000900:Anti-Bacterial Agents / Q000737:chemistry; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D012997:Solvents; D017334:Teicoplanin / Q000031:analogs & derivatives / Q000737:chemistry; D013696:Temperature
D000900:Anti-Bacterial Agents; D002021:Buffers; D012997:Solvents; D017334:Teicoplanin; C096602:MDL 63246