Increased VH 11 and VH Q52 gene use by splenic B cells in old mice associated with oligoclonal expansions of CD5 + B cells.
A significant increase in the utilization of the VH gene families VH11 and Q52 was observed in LPS-stimulated splenic B lymphocytes from aged mice compared to young mice. VH gene usage was assayed by in situ DNA/RNA hybridization using VH family-specific and kappa chain probes. The observed age-dependent differences appear to reflect the preferential use of VH11 and Q52 VH gene use by the CD5 + B lymphocyte subset whose numbers in the spleen increase with age. The increased use of VH11 by splenic cells from old mice is associated with clonal expansions of splenic CD5 + B lymphocytes.
Department of Medicine, Hadassah University Hospital, Ein-Karem, Jerusalem, Israel.
A
A
Ben-Yehuda
P
P
Szabo
J
J
LeMaoult
J S
JS
Manavalan
M E
ME
Weksler
D000375:Aging / Q000235:genetics / Q000502:physiology*; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000502:physiology*; D001402:B-Lymphocytes / Q000502:physiology*; D018956:CD5 Antigens / Q000502:physiology; D002478:Cells, Cultured; D005787:Gene Frequency; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000166:cytology
D018956:CD5 Antigens; D007143:Immunoglobulin Heavy Chains