Development of a new culture medium for the rapid detection of Salmonella by indirect conductance measurements.
The main difficulties in conductance medium development are to allow Salmonella to grow and produce a conductance signal while impeding growth of related species such as Escherichia coli and Citrobacter freundii. Various selective agents were screened for these capacities and a new medium was derived, named KIMAN (Whitley Impedance Broth basal medium supplemented with three selective components: novobiocin, malachite green and potassium iodide). This medium supported the growth of Salmonella serotypes and inhibited non-salmonella strains in pure cultures.
Centre National d'Etudes Vétérinaires et Alimentaires, Ploufragan, France.
D
D
Blivet
G
G
Salvat
F
F
Humbert
P
P
Colin
D001431:Bacteriological Techniques; D003470:Culture Media; D004553:Electric Conductivity; D012475:Salmonella / Q000302:isolation & purification*
D003470:Culture Media