Estrogens modulate bovine vascular endothelial cell permeability and HSP 25 expression concomitantly.
The atheroprotective properties of estrogens are supported by clinical data from postmenopausal women who use estrogen replacement therapy. However, the mechanisms mediating activity remain unknown, and it has been suggested that estrogens may help to modulate endothelial permeability to atherogenic lipoproteins. In these studies we used bovine vascular endothelial cells as an in vitro model to show that estrogens were able to regulate low-density lipoprotein transport and permeability of the endothelial monolayer. Macromolecular transport was observed to be a second-order polynomial function of estrogen concentration. Moreover, this regulation was correlated with expression of heat shock protein (HSP) 25, which is known to influence fluid phase pinocytosis and cytoskeleton remodeling, thus suggesting a role for HSP 25 in the estrogenic control of transcellular permeability of the endothelium monolayer.
Institut National de la Santé et de la Recherche Médicale Unité 397, Institut L. Bugnard, Centre Hospitalier Universitaire (CHU) Rangueil, 31403 Toulouse Cedex 4, France.
F
F
Delarue
S
S
Daunes
R
R
Elhage
A
A
Garcia
F
F
Bayard
J
J
Faye
D000818:Animals; D001011:Aorta; D001692:Biological Transport / Q000187:drug effects; D015153:Blotting, Western; D002417:Cattle; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000502:physiology; D004730:Endothelium, Vascular / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D006801:Humans; D007700:Kinetics; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics*; D010873:Pinocytosis
D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D008077:Lipoproteins, LDL; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D004997:Ethinyl Estradiol