Automated assay of gene expression at cellular resolution.
We have recently developed an automated image processing method for obtaining quantitative values for average levels of gene expression at the resolution of a single cell. This method is described in the present paper. We place this method within a larger framework for the study of gene regulation in Drosophila, stressing that gene circuit models and improved data processing methods are mutually reinforcing approaches to this problem.
Kosman
D
D
Brookdale Center for Molecular Biology, Mt. Sinai Medical School, New York, NY 10029, USA.
Reinitz
J
J
Sharp
D H
DH
D000818:Animals; D001331:Automation; D001756:Blastoderm / Q000502:physiology; D002477:Cells / Q000378:metabolism; D003198:Computer Simulation; D004268:DNA-Binding Proteins / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods; D019476:Insect Proteins / Q000235:genetics; D051741:Kruppel-Like Transcription Factors; D008957:Models, Genetic; D012097:Repressor Proteins; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D016335:Zinc Fingers
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D051741:Kruppel-Like Transcription Factors; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C056478:bcd protein, Drosophila; C061245:hb protein, Drosophila