Inhibition of human immunodeficiency type 1 virus replication in monocytic U-937 cells by superinfection with Chlamydia trachomatis.
The aim of this study was to investigate the effects of infection of monocytic cells with both the human immunodeficiency type 1 virus (HIV-1) and Chlamydia trachomatis on the replication of each pathogen. U-937 cells, chronically infected with HIV-1 (strain LaVLai), either induced to differentiate into immature macrophage-like cells by 32 pM 12-O-tetradecanol phorbol-13-acetate or uninduced, were superinfected with C. trachomatis serovar L2. Both HIV-1 infection and differentiation rendered the U-937 cells highly susceptible to C. trachomatis lytic infection. Differentiation and superinfection of HIV-1-infected cells with C. trachomatis both affected cell viability and reduced viral production in vitro. RT activity was one tenth the original value after differentiation of HIV-1-infected cells, one twentieth the original value after superinfection with C. trachomatis, and one hundredth the original value after differentiation and superinfection with C. trachomatis.
Laboratoire de Bactériologie Virologie-Hygiène, Hôpital Saint-Louis, Université Paris VII, France.
A
A
Bianchi
F
F
Ferchal
J
J
Lejunter
C
C
Scieux
D000898:Antibiosis; D002454:Cell Differentiation; D002460:Cell Line / Q000166:cytology / Q000382:microbiology / Q000821:virology; D002470:Cell Survival; D002692:Chlamydia trachomatis / Q000502:physiology*; D019085:Fluorescent Antibody Technique, Direct; D015497:HIV-1 / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D002479:Inclusion Bodies; D008214:Lymphocytes; D008854:Microscopy, Electron; D014779:Virus Replication