Plant derived estrogens relax rat mesenteric artery in vitro.
The aim of this study was to investigate whether plant derived estrogens have the same relaxing effects in vitro as estradiol-17beta on arterial smooth muscle. The mesenteric arterial rings of female and male Wistar rats were studied. The relaxing effects of estradiol-17beta, genistein, daidzein and beta-sitosterol were determined, with particular focus on the role of endothelium. B-sitosterol had no relaxing effect on the arteries. Estradiol-17beta, genistein and daidzein relaxed noradrenaline, potassium chloride and calcium chloride precontracted arterial rings endothelium-independently. The relaxation responses were also independent of gender. Neither the removal of endothelium, nor the inhibition prostacyclin or nitric oxide synthesis, had any effect on the relaxation responses. The exact mechanism of these findings is still unclear.
Nevala
R
R
Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki, Finland.
Korpela
R
R
Vapaatalo
H
H
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000302:isolation & purification* / Q000494:pharmacology; D005260:Female; D019833:Genistein / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007529:Isoflavones / Q000494:pharmacology; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D009131:Muscle, Smooth, Vascular / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012855:Sitosterols / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004967:Estrogens; D007529:Isoflavones; D010936:Plant Extracts; D012855:Sitosterols; D014662:Vasoconstrictor Agents; D004958:Estradiol; C025473:gamma-sitosterol; C004742:daidzein; D011189:Potassium Chloride; D019833:Genistein; D002122:Calcium Chloride; D019331:NG-Nitroarginine Methyl Ester; D009638:Norepinephrine; D007213:Indomethacin