Low molecular weight phosphotyrosine protein phosphatase translocation during cell stimulation with platelet-derived growth factor.
Low Mr phosphotyrosine protein phosphatase (PTP) is a cytosolic enzyme whose activity upon platelet-derived growth factor (PDGF) and insulin receptors has been demonstrated in vivo. In our study we demonstrate that this enzyme, both naturally expressed and overexpressed in NIH/3T3 fibroblasts, translocates from the cytosol to the Triton X-100 insoluble fraction following stimulation with PDGF. It emerges that the phosphorylation of a defined population of PDGF receptors, which is localized in this fraction and seems to be endowed with peculiar features and functions, is particularly affected by low Mr PTP overexpression.
Rigacci
S
S
Department of Biochemical Sciences, University of Florence, Italy.
Bucciantini
M
M
Marzocchini
R
R
Berti
A
A
D016475:3T3 Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D000077214:Becaplermin; D001692:Biological Transport; D002455:Cell Division / Q000187:drug effects; D003600:Cytosol / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D008970:Molecular Weight; D017830:Octoxynol; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology*; D017027:Protein Tyrosine Phosphatases / Q000187:drug effects* / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000187:drug effects / Q000378:metabolism; D017479:Receptors, Platelet-Derived Growth Factor / Q000187:drug effects / Q000378:metabolism; D012995:Solubility; D013347:Subcellular Fractions / Q000737:chemistry / Q000187:drug effects / Q000201:enzymology
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D017830:Octoxynol; D017479:Receptors, Platelet-Derived Growth Factor; D016392:Proto-Oncogene Proteins pp60(c-src); D017027:Protein Tyrosine Phosphatases