S-[(1 and 2)-phenyl-2-hydroxyethyl]cysteine-induced alterations in renal mitochondrial function in male Fischer-344 rats.
Previous studies from our laboratory have shown that mitochondrial dysfunction may be an important early event in S-[(1 and 2)-phenyl-2-hydroxyethyl]cysteine (PHEC)-induced cytotoxicity in isolated rat renal proximal tubules. The present study has therefore examined in more detail PHEC-induced mitochondrial dysfunction, both in vivo and in vitro, using isolated renal cortical mitochondria. Renal cortical mitochondria isolated from PHEC-treated rats in vivo showed depressed effects on the mitochondrial respiration and oxidative phosphorylation in both a dose (0, 250, and 500 micromol/kg iv)- and time (0-24 h)-dependent manner in the presence of both succinate (Site 2) and malate plus alpha-ketoglutarate (Site 1) as respiratory substrates, with initial significant depression occurring as early as 4 h following treatment with 500 micromol PHEC/kg. Similar mitochondrial dysfunctions were observed in vitro in concentration- and time-dependent manners with both respiratory substrates. PHEC also caused a marked dose-dependent inhibition of mitochondrial succinate dehydrogenase and NADH cytochrome c reductase activities both in vivo and in vitro, with initial inhibition occurring as early as 4 h after in vivo administration and 45 min after exposure to PHEC in vitro, while the NADH dehydrogenase activity was not considerably inhibited. The mitochondrial ATPase activity was significantly decreased 4 and 24 h following treatment with PHEC (500 micromol/kg). These results suggest that PHEC exerts its inhibitory effect on the mitochondrial respiration and oxidative phosphorylation through the action on the mitochondrial electron transport chain. PHEC significantly reduced the activity of adenine nucleotide translocase as well as the net uptake of substrates by mitochondria without affecting their efflux within 2-4 h after its injection (500 micromol/kg). On the other hand, significant renal damage, as assessed by morphological study, appeared as early as 24 h following such treatment. The observation of similar effects after both in vivo and in vitro exposures may suggest that the effect on mitochondria may have a pathogenic role in PHEC-induced renal injury in rats. PHEC produces mitochondrial toxicity that results from an inactivation of mitochondrial anionic substrate transporters as well as from an inhibition of activities of adenine nucleotide translocase and dehydrogenases.
Chakrabarti
S K
SK
Facultéde Médecine, Universitéde Montréal, Montréal, Québec, H3C 3J7, Canada.
Denniel
C
C
Malick
M A
MA
Bai
C
C
D000818:Animals; D003545:Cysteine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000633:toxicity*; D066298:In Vitro Techniques; D007275:Injections, Intravenous; D007656:Ketoglutaric Acids / Q000494:pharmacology; D007672:Kidney Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008293:Malates / Q000494:pharmacology; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D000226:Mitochondrial ADP, ATP Translocases / Q000037:antagonists & inhibitors; D009245:NADH Dehydrogenase / Q000037:antagonists & inhibitors; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D011916:Rats, Inbred F344; D013385:Succinate Dehydrogenase / Q000037:antagonists & inhibitors; D019802:Succinic Acid / Q000494:pharmacology
D004791:Enzyme Inhibitors; D007656:Ketoglutaric Acids; D008293:Malates; C101345:S-(1-phenyl-2-hydroxyethyl)cysteine; C030298:malic acid; D000226:Mitochondrial ADP, ATP Translocases; D019802:Succinic Acid; D013385:Succinate Dehydrogenase; D009245:NADH Dehydrogenase; D003545:Cysteine