K+ ionophores stimulate retrovirus-induced fusion-from-within.
Fusion-from-without (FFWO) and fusion-from-within (FFWI) for Moloney MLV in SC-1 cells were selectively stimulated by the polycation polybrene and the ionophore amphotericin B, which can be used to discriminate between the two fusion types. FFWI was stimulated by a number of different K+ ionophores. The stimulation occurred within few hours, did not require protein synthesis, and depended on the uninfected cell type, which suggests that FFWI is stimulated by a permutation of the K+ gradient of the uninfected cell. Plasma membrane vesicles from infected cells could also stimulate fusion with the same stimulation pattern as FFWI.
Andersen
K B
KB
Department of Pharmacology, Royal Danish School of Phamacy, Copenhagen. kba@mail.dfh.dk
D016475:3T3 Cells; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D015686:Gene Products, env / Q000378:metabolism; D006801:Humans; D007476:Ionophores / Q000494:pharmacology*; D007700:Kinetics; D008561:Membrane Fusion; D051379:Mice; D008979:Moloney murine leukemia virus / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology*
D015686:Gene Products, env; D007476:Ionophores; D000666:Amphotericin B; D011188:Potassium