Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP).
Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.
Department of Veterinary Radiology, School of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
A
A
Asaeda
H
H
Ide
K
K
Tano
Y
Y
Takamori
K
K
Kubo
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000447:Aldehydes / Q000032:analysis; D001710:Biotin / Q000031:analogs & derivatives; D019757:Carbon-Oxygen Lyases / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D045647:DNA Glycosylases; D004260:DNA Repair / Q000235:genetics*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016296:Mutagenesis / Q000235:genetics; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured
D000447:Aldehydes; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D001710:Biotin; D004247:DNA; D008741:Methyl Methanesulfonate; D043223:Deoxyribonuclease IV (Phage T4-Induced); D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D000225:Adenine