Characterization of a breast cancer cell line derived from a germ-line BRCA1 mutation carrier.
A tumor cell line, HCC1937, was established from a primary breast carcinoma from a 24-year-old patient with a germ-line BRCA1 mutation. A corresponding B-lymphoblastoid cell line was established from the patient's peripheral blood lymphocytes. BRCA1 analysis revealed that the tumor cell line is homozygous for the BRCA1 5382insC mutation, whereas the patient's lymphocyte DNA is heterozygous for the same mutation, as are at least two other family members' lymphocyte DNA. The tumor cell line is marked by multiple additional genetic changes including a high degree of aneuploidy, an acquired mutation of TP53 with wild-type allele loss, an acquired homozygous deletion of the PTEN gene, and loss of heterozygosity at multiple loci known to be involved in the pathogenesis of breast cancer. Comparison of the primary tumor with the cell line revealed the same BRCA1 mutation and an identical pattern of allele loss at multiple loci, indicating that the cell line had maintained many of the properties of the original tumor. This breast tumor-derived cell line may provide a useful model system for the study of familial breast cancer pathogenesis and for elucidating BRCA1 function and localization.
Tomlinson
G E
GE
Department of Pediatrics, and the Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235-8593, USA. tomlinson@simmons.swmed.edu
Chen
T T
TT
Stastny
V A
VA
Virmani
A K
AK
Spillman
M A
MA
Tonk
V
V
Blum
J L
JL
Schneider
N R
NR
Wistuba
I I
II
Shay
J W
JW
Minna
J D
JD
Gazdar
A F
AF
D000328:Adult; D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D004273:DNA, Neoplasm / Q000235:genetics; D005091:Exons; D005260:Female; D019398:Genes, BRCA1; D018095:Germ-Line Mutation; D006579:Heterozygote; D006801:Humans; D007621:Karyotyping; D010375:Pedigree; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm