Comparisons of two polymorphic species of Ostertagia and phylogenetic relationships within the Ostertagiinae (Nematoda: Trichostrongyloidea) inferred from ribosomal DNA repeat and mitochondrial DNA sequences.
The first internal transcribed spacer DNA (ITS-1) (rDNA) and the mitochondrial (mt) DNA-derived cytochrome oxidase I gene (COX-1) were enzymatically amplified, cloned and sequenced from 6 nominal species of Ostertagiinae as well as Haemonchus contortus and Haemonchus placei. The portion of the COX-1 gene analyzed was 393 base pairs (bp) in length and contained 33 within species polymorphic base changes at 28 synonymous sites. The ITS-1 rDNA consensus sequences ranged from 392 bp (Ostertagia ostertagi/Ostertagia lyrata, Teladorsagia circumcincta) to 404 bp (H. contortus, H. placei). These data were used both in a distance analysis to assess the concept of polymorphic species within the genus Ostertagia and in parsimony analysis to assess phylogenetic relationships within a limited group of Ostertagiinae. Pairwise similarity scores of both ITS-1 and COX-1 data showed the highest number of conserved sites between the proposed dimorphic species of Ostertagia. The level of similarity was lower in the COX-1 data due to the high number of synonymous base changes. Analysis by maximum parsimony of the same data did not refute O. ostertagi/O. lyrata and Ostertagia mossil/Ostertagia dikmansi as dimorphic species and supported monophyly of these ostertagiines relative to representatives of the haemonchine outgroup. In the single most parsimonious tree from ITS-1 rDNA data, a subclade of Ostertagia spp. included forms possessing parallel synlophes and long esophageal valves that typically occur in cervid hosts.
Zarlenga
D S
DS
U.S. Department of Agriculture, ARS, Immunology and Disease Resistance Laboratory, Beltsville, Maryland 20705, USA.
Hoberg
E P
EP
Stringfellow
F
F
Lichtenfels
J R
JR
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018509:DNA, Helminth / Q000737:chemistry*; D004272:DNA, Mitochondrial / Q000737:chemistry*; D004275:DNA, Ribosomal / Q000737:chemistry*; D003670:Deer; D003576:Electron Transport Complex IV / Q000235:genetics; D005260:Female; D008297:Male; D008969:Molecular Sequence Data; D010028:Ostertagia / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment
D018509:DNA, Helminth; D004272:DNA, Mitochondrial; D004275:DNA, Ribosomal; D003576:Electron Transport Complex IV