Detection of mycoplasmas in urethral swabs from HIV-1 infected patients and control individuals using culture techniques and polymerase chain reaction.
The objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma penetrans, in urethral swabs from HIV-1 infected patients compared to swabs from a control group. Mycoplasmas were detected by routine culture techniques and by the Polymerase Chain Reaction (PCR) technique, using 16SrRNA generic primers of conserved region and Mycoplasma penetrans specific primers. The positivity rates obtained with the two methods were comparable. Nevertheless, PCR was more sensitive, while the culture techniques allowed the quantification of the isolates. The results showed no significant difference (p < 0.05) in positivity rates between the methods used for mycoplasma detection.
da Cunha
R A
RA
Faculdade de Ciências Farmacêuticas da Universidade de São Paulo, Brazil.
Takei
K
K
Vaz
A J
AJ
Rosenthal
C
C
D000163:Acquired Immunodeficiency Syndrome / Q000382:microbiology*; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics; D015497:HIV-1; D006801:Humans; D008297:Male; D009174:Mycoplasma / Q000235:genetics / Q000302:isolation & purification*; D019535:Mycoplasma hominis / Q000235:genetics / Q000302:isolation & purification; D018661:Mycoplasma penetrans / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015995:Prevalence; D012680:Sensitivity and Specificity; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000302:isolation & purification; D014521:Urethra / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial