[Recombinational origin of natural transposable phages of related species belonging to group B3, active in Pseudomonas aeruginosa species].
A heteroduplex analysis of four related transposable phages--B3, PM57, PM62, and Hw12--of the Pseudomonas aeruginosa B3 group was performed. Heteroduplex structures, restriction maps, and data on DNA-DNA hybridization obtained upon hybridization of phage DNA restriction fragments with labeled probes representing different regions of the phage genomes are in good agreement. The data obtained strongly confirmed the recombinational origin of the analyzed phages. Thus, all natural transposable phages of P. aeruginosa, including phages from both group B3 and species D3112, were shown to have a recombinational origin.
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
V Z
VZ
Akhverdian
A O
AO
Lobanov
E A
EA
Khrenova
V N
VN
Krylov
D004251:DNA Transposable Elements; D009692:Nucleic Acid Heteroduplexes; D009693:Nucleic Acid Hybridization; D017105:Pseudomonas Phages / Q000235:genetics*; D011550:Pseudomonas aeruginosa / Q000821:virology*; D011995:Recombination, Genetic; D015183:Restriction Mapping
D004251:DNA Transposable Elements; D009692:Nucleic Acid Heteroduplexes