Separation of lac dye components by high-speed counter-current chromatography.
High-speed counter-current chromatography has been successfully applied to the separation of the lac dye components. A 25-mg quantity of the sample was separated using a two-phase solvent system composed of tert.-butyl methyl ether-n-butanol-acetonitrile-water (2:2:1:5). The fractions were analyzed by high-performance liquid chromatography and electrospray tandem mass spectrometry. The separation yielded 2.6 mg of 97.2% pure laccaic acid C, 9.5 mg of 98.1% pure laccaic acid A, 3.6 mg of 98.2% pure laccaic acid B, and 0.5 mg of a 95.0% pure anthraquinonedicarboxylic acid with a molecular mass of 360.
Aichi Prefectural Institute of Public Health, Nagoya, Japan.
H
H
Oka
Y
Y
Ito
S
S
Yamada
T
T
Kagami
J
J
Hayakawa
K
K
Harada
E
E
Atsumi
M
M
Suzuki
M
M
Suzuki
H
H
Odani
S
S
Akahori
K
K
Maeda
H
H
Nakazawa
Y
Y
Ito
D001391:Azo Compounds / Q000302:isolation & purification*; D002845:Chromatography; D003377:Countercurrent Distribution; D005505:Food Coloring Agents / Q000302:isolation & purification*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D012997:Solvents
D001391:Azo Compounds; D005505:Food Coloring Agents; D007202:Indicators and Reagents; D012997:Solvents; C009765:lac dye