Evidence that the E2-isoprostane, 15-E2t-isoprostane (8-iso-prostaglandin E2) is formed in vivo.
D2/E2-isoprostanes are prostaglandin D2/E2-like compounds that are produced in vivo as non-enzymatic products of free radical catalyzed peroxidation of arachidonic acid. One E2-isoprostane that should be formed is 15-E2t-isoprostane (8-iso-prostaglandin E2). 15-E2t-isoprostane has been shown to exert potent biological activity but proof that it is formed in vivo is lacking. Evidence is now presented that 15-E2t-isoprostane is formed in vivo by demonstrating that an endogenous E2-isoprostane with a retention time on capillary GC identical with that of 15-E2t-isoprostane co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabeled 15-E2t-isoprostane.
Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232-6602, USA.
J D
JD
Morrow
J
J
Scruggs
Y
Y
Chen
W E
WE
Zackert
L J
LJ
Roberts
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000031:analogs & derivatives* / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification; D005609:Free Radicals / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D028421:Isoprostanes; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D005609:Free Radicals; D028421:Isoprostanes; D016718:Arachidonic Acid; D015232:Dinoprostone; C011987:8-isoprostaglandin E2