Indirect measurement of Epstein-Barr virus neutralising antibodies by ELISA.
A rapid and effective ELISA for measuring Epstein-Barr virus (EBV)-neutralizing antibodies in human sera was devised to replace the existing cumbersome method involving the inhibition of fetal cord blood B-cell transformation by the virus. The new method will be invaluable for assessing antibody responses in human subjects participating in EBV gp340 vaccine trials. The ELISA developed uses the human serum antibody to be tested to inhibit standardised binding of an EBV-neutralizing monoclonal antibody (mAb) to gp340 itself or its recombinant derivatives. A serum which has high EBV-neutralizing antibody titres inhibits the binding of neutralizing mAb to gp340 more than a serum with low levels. EBV neutralisation antibody titres obtained by the new inhibition ELISA correlate well with values obtained using the lengthy conventional assay where inhibition of B-cell transformation is assessed. The new assay can be carried out in a few hours compared to 4-5 weeks for the conventional test and could be automated for processing very large sample numbers in vaccine trials.
Department of Pathology and Microbiology, School of Medical Sciences, University of Bristol, UK.
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Wilson
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Morgan
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood* / Q000276:immunology; D001667:Binding, Competitive; D002472:Cell Transformation, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005312:Fetal Blood / Q000166:cytology / Q000821:virology; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000737:chemistry / Q000276:immunology; D008214:Lymphocytes / Q000821:virology; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D012044:Regression Analysis; D014763:Viral Matrix Proteins / Q000276:immunology*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D007106:Immune Sera; D011994:Recombinant Proteins; D014763:Viral Matrix Proteins