Interactions of signaling pathways in ACTH (1-24)-induced cell shape changes in invertebrate immunocytes.
ACTH (1-24) induces cell shape changes in the immunocytes of the bivalve mollusc, Mytilus galloprovincialis. Using computer-assisted microscopic image analysis, we have found that the G protein antagonist suramin sodium, the adenylate cyclase inhibitor 2',5'-dideoxyadenosine, and the protein kinase inhibitor staurosporine inhibit this effect. The highly specific inhibitors H-89 (for protein kinase A) and calphostin C (for protein kinase C) only inhibited partially the morphological alterations. In contrast, the simultaneous action of H-89 and calphostin C completely blocked these changes. The above findings indicate that ACTH (1-24) induces cell shape changes in molluscan immunocytes via adenylate cyclase/cAMP/protein kinase A pathway, as well as the activation of protein kinase C.
Department of Animal Biology, University of Modena, Italy.
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D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D049872:Bivalvia; D048429:Cell Size / Q000187:drug effects*; D003366:Cosyntropin / Q000494:pharmacology*; D016048:Dideoxyadenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019204:GTP-Binding Proteins / Q000037:antagonists & inhibitors; D006434:Hemocytes / Q000187:drug effects*; D006458:Hemolymph / Q000166:cytology; D007091:Image Processing, Computer-Assisted; D007546:Isoquinolines / Q000494:pharmacology; D008858:Microscopy, Phase-Contrast; D009281:Naphthalenes / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D015398:Signal Transduction / Q000187:drug effects*; D019311:Staurosporine / Q000494:pharmacology; D013449:Sulfonamides; D013498:Suramin / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D007546:Isoquinolines; D009281:Naphthalenes; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; D003366:Cosyntropin; D016048:Dideoxyadenosine; D013498:Suramin; C021008:2',5'-dideoxyadenosine; D019204:GTP-Binding Proteins; D019311:Staurosporine; C058819:calphostin C; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide