Involvement of Galphai2 in the maintenance and biogenesis of epithelial cell tight junctions.
Polarized epithelial cells have highly developed tight junctions (TJ) to maintain an impermeant barrier and segregate plasma membrane functions, but the mechanisms that promote TJ formation and maintain its integrity are only partially defined. Treatment of confluent monolayers of Madin-Darby canine kidney (MDCK) cells with AlF4- (activator of heterotrimeric G protein alpha subunits) results in a 3-4-fold increase in transepithelial resistances (TER), a reliable indicator of TJ integrity. MOCK cells transfected with activated Galpha0 (Q205L) have acclerated TJ formation (Denker, B. M., Saha, C. , Khawaja, S., and Nigam, S. J. (1996) J. Biol. Chem. 271, 25750-25753). Galphai2 has been localized within the tight junction, and a role for Galphai2 in the formation and/or maintenance of the tight junction was studied by transfection of MDCK cells with vector without insert (PC), wild type Galphai2, or a GTPase-deficient mutant (constitutively activated), Q205Lalphai2. Tryptic conformational analysis confirmed expression of a constitutively active Galphai2 in Q205Lalphai2-MDCK cells, and confocal microscopy showed a similar pattern of Galphai2 localization in the three cell lines. Q205Lalphai2-MDCK cells had significantly higher base-line TER values than wild type Galphai2- or PC-MDCK cells (1187 +/- 150 versus 576 +/- 89 (Galphai2); 377 +/- 52 Omega.cm2 (PC)), and both Galphai2- and Q205Lalphai2-transfected cell lines more rapidly develop TER in the Ca2+ switch, a model widely used to study the mechanisms of junctional assembly. Treatment of cells with AlF4- during the Ca2+ switch had little effect on the kinetics of TER development in Galphai2- or Q205Lalphai2-MDCK cells, but PC cells reached half-maximal TER significantly sooner in the presence of AlF4- (similar times to Galphai2-transfected cells). Base-line TER values obtained after the switch were significantly higher for all three cell lines in the presence of AlF4-. These findings indicate that Galphai2 is important for both the maintenance and development of the TJ, although additional Galpha subunits are likely to play a role.
Saha
C
C
Renal Division, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA.
Nigam
S K
SK
Denker
B M
BM
D017607:Aluminum Compounds / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects; D005459:Fluorides / Q000494:pharmacology; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D019204:GTP-Binding Proteins / Q000494:pharmacology / Q000502:physiology*; D007668:Kidney / Q000166:cytology; D018613:Microscopy, Confocal; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D019108:Tight Junctions / Q000187:drug effects / Q000502:physiology*; D014162:Transfection; D014357:Trypsin / Q000378:metabolism
D017607:Aluminum Compounds; D011518:Proto-Oncogene Proteins; C050992:tetrafluoroaluminate; D014357:Trypsin; D019204:GTP-Binding Proteins; D051960:GTP-Binding Protein alpha Subunit, Gi2; D019206:GTP-Binding Protein alpha Subunits, Gi-Go; D005459:Fluorides; D002118:Calcium