Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer.
In human endometrial cancer, we have previously identified a 790-kb region of common allelic loss in chromosome bands 10q25-q26, flanked by D10S587 and D10S1723. We constructed a contig covering the entire deleted region using YACs, PACs, and BACs. Five overlapping cosmid clones derived from YAC clones completely covered the entire deleted region: its size was estimated to be no larger than 200 kb. We further performed two-color fluorescence in situ hybridization (FISH) analysis to confirm the deletion and narrowed down the deleted region to 100 kb or less; it was covered by three overlapping cosmid clones that were included in one BAC clone. Restriction endonuclease mapping identified a region in which NotI, SalI, SmaI, and Xhol were clustered, suggesting the possible existence of a CpG island.
Yamakawa
H
H
Department of Molecular Pathology, Tohoku University School of Medicine, Sendai, Japan.
Nagase
S
S
Yuki
M
M
Shiwaku
H O
HO
Furukawa
T
T
Yoshinaga
K
K
Soeda
E
E
Hoshi
M
M
Hayashi
Y
Y
Sato
S
S
Yajima
A
A
Horii
A
A
D002871:Chromosome Banding; D002873:Chromosome Fragility; D018244:Chromosomes, Artificial, Yeast; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D003001:Cloning, Molecular; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019656:Loss of Heterozygosity; D015183:Restriction Mapping