Further studies on vertebrate S-type lectins: cross-reactivity between toad and human lectins.
Galectins (S-type or S-Lac lectins) are a well-defined family of beta-galactoside animal lectins characterized by a high sequence homology in the carbohydrate-binding domain. We have previously purified and characterized the S-type lectin from the ovary of the toad Bufo arenarum. In this study, we purified the S-type lectins from Bufo arenarum ovary and human spleen by an improved method which included ion exchange and affinity chromatography. Antibody cross-reactivities between both lectins and some other S-type lectins showed that they share epitopes. Glycosylation studies carried out with detection/differentiation kits suggested that both lectins are not glycosylated.
Elola
M T
MT
Departamento de Ciencias Biológicas, Universidad Nacional de La Plata, Argentina.
Fink
N E
NE
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002022:Bufo arenarum; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D006031:Glycosylation; D006801:Humans; D037102:Lectins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D010053:Ovary / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D037102:Lectins