Cell cycle dependency of porphyrin uptake in a glioma cell line.
We used a two-color analysis system to assess the porphyrin uptake and DNA content in four established cell lines of glioma employing flow cytometry (FCM). The FCM study revealed porphyrin uptake in all cells, regardless of the phase of the cell cycle they were in. However, those in the G0/G1 phase showed moderate uptake of porphyrin and those in the G2/M phase showed a higher uptake. These results indicated the advantage of using porphyrin as the carrier of tumor targeting agents as a therapeutic strategy for malignant tumors.
Department of Neurosurgery, University of Tsukuba, Ibaraki, Japan.
Y
Y
Shibata
A
A
Matsumura
F
F
Yoshida
T
T
Yamamoto
K
K
Nakai
T
T
Nose
I
I
Sakata
S
S
Nakajima
D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D004273:DNA, Neoplasm / Q000032:analysis; D005434:Flow Cytometry; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008856:Microscopy, Fluorescence; D011166:Porphyrins / Q000493:pharmacokinetics*; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D011166:Porphyrins