Three new dominant C1 suppressor alleles in Zea mays.
Three new dominant suppressor mutations of the C1 transcription regulator gene in maize--C1-I delta 1, C1-I delta 2 and C1-I delta 3--are described that suppress anthocyanin colouration in kernels similar to the function of the C1-I standard inhibitor. The C1-I delta mutations were induced by imprecise excision of an En/Spm transposon in the third exon of the C1 gene. These transposon footprints cause a frameshift in the C1 open reading frame that leads to truncated proteins due to an early stop codon 30 amino acids upstream of the wild-type C1 protein. Therefore, the C1-I delta gene products lack the carboxy-terminal transcriptional activation domain of C1. The C1-I standard allele also lacks this domain and in addition differs in 17 amino acids from the wild-type C1 allele. The new C1-I delta alleles provide evidence that deletion of the carboxy-terminal activation domain alone is sufficient to generate a dominant suppressive effect on the function of wild-type C1.
Lehrstuhl für Genetik, Technische Universität München, Germany.
T
T
Singer
A
A
Gierl
P A
PA
Peterson
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics*; D005799:Genes, Dominant; D016153:Genes, Suppressor; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D003313:Zea mays / Q000235:genetics*
C067401:C1 protein, Zea mays; D017931:DNA Primers; D004268:DNA-Binding Proteins; D010940:Plant Proteins; D014157:Transcription Factors