Enantiomer separation by pressure-supported electrochromatography using capillaries packed with a permethyl-beta-cyclodextrin stationary.
Efficient enantiomer separation by pressure-assisted, micro-packed capillary electrochromatography (CEC) has been carried out using a permethyl-beta-cyclodextrin-modified silica support (PM-beta-CD-silica). When comparing this method with micro-packed-high-performance liquid chromatography in the single-column-mode, CEC displays higher column efficiencies (about three times higher theoretical plate numbers at comparable elution times). The pressure support (about 10 bar), applied to avoid bubble formation, has a negligible influence on elution times in CEC. The influence of the type and composition of organic modifiers is described.
Institute of Organic Chemistry, University of Tübingen, Germany.
D
D
Wistuba
H
H
Czesla
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M
Roeder
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V
Schurig
D002851:Chromatography, High Pressure Liquid; D003505:Cyclodextrins; D019075:Electrophoresis, Capillary; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D004364:Pharmaceutical Preparations; D047392:beta-Cyclodextrins; C031215:betadex