Identification of a novel adhesin-like glycoprotein from Mycoplasma hyopneumoniae.
This study identifies an adhesin-like glycoprotein, which was a 110 kDa protein (P110) under HPLC-GPC assay. This adhesin consisted of one P54 and two P28 subunits. In addition, N-glycosidase F could cleave all N-linked oligosaccharides on the P54 subunit. Experimental results indicated that P110 with native conformations significantly inhibited the adherence of biotin-labeled porcine tracheal epithelial cell extracts to the intact M. hyopneumoniae cells (p < 0.01). Furthermore, the biotin-labeled porcine tracheal epithelial cell extracts specifically bound to P54 and P28 subunits. This binding could be competitively inhibited by unlabeled porcine tracheal epithelial extracts and SPF porcine antisera against Mycoplasma hyopneumoniae. Both P54 and P28 subunits were constitutively expressed in different strains of M. hyopneumoniae. Their production was negligibly changed at various passages during in vitro cultivation. The significant role of this adhesin-like glycoprotein in the pathogenesis of swine pneumonia is under study.
Chen
J R
JR
Department of Pathobiology, Pig Research Institute Taiwan, Chunan, Miaoli, Taiwan.
Lin
J H
JH
Weng
C N
CN
Lai
S S
SS
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000276:immunology*; D000581:Amidohydrolases / Q000737:chemistry; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal; D001667:Binding, Competitive / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002846:Chromatography, Affinity / Q000662:veterinary; D002850:Chromatography, Gel / Q000662:veterinary; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D007104:Immune Adherence Reaction / Q000662:veterinary; D051379:Mice; D008970:Molecular Weight; D009174:Mycoplasma / Q000737:chemistry / Q000276:immunology*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology / Q000662:veterinary*; D011817:Rabbits; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*; D014132:Trachea / Q000276:immunology
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase