Expression of functional MHC class II molecules by a mouse pro-B cell clone.
We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic mouse. This clone has the phenotype B220+, BP-1+, HSA+, CD43+, lambda5+, and CD25-, and has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in an MHC class II-restricted presentation.
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS et Unité 184 de I'INSERM, Institut de Chimie Biologique, Strasbourg, France.
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Lombard-Platet
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Fisher
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Meyer
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Ceredig
D000818:Animals; D000911:Antibodies, Monoclonal; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells; D005260:Female; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis* / Q000502:physiology; D016393:Lymphoma, B-Cell; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D013234:Stem Cells / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic / Q000276:immunology; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D010455:Peptides; D009113:Muramidase