PCNA binding proteins in Drosophila melanogaster : the analysis of a conserved PCNA binding domain.
The eukaryotic polymerase processivity factor, PCNA, interacts with cell cycle regulatory proteins such as p21(WAF1/Cip1) and Gadd45, as well as with proteins involved in the mechanics of DNA repair and replication. A conserved PCNA-binding motif is found in a subset of PCNA-interacting proteins, including p21, suggesting that the regulation of these interactions is important for the co-ordination of DNA replication and repair. We have identified several classes of protein which bind to Drosophila PCNA. Two of these proteins contain the consensus PCNA-binding domain: one is the Dacapo protein, a Drosophila homologue of p21(WAF1/Cip1), and the second is the transposase encoded by the Pogo DNA transposon . A conserved PCNA-binding domain is also present in a human relative of Pogo , named Tigger , suggesting that this domain has a functional role in this class of transposable element. This raises interesting possibilities for a novel method of transposition in which the transposase might be targeted to replicating DNA. Finally, we have investigated the use of this conserved PCNA-binding domain as a predictor of PCNA-binding capacity.
Warbrick
E
E
CRC Laboratories, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK. ewarbrick@dundee.ac.uk
Heatherington
W
W
Lane
D P
DP
Glover
D M
DM
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D016384:Consensus Sequence; D017124:Conserved Sequence; D045647:DNA Glycosylases; D000072481:DNA Ligase ATP; D011088:DNA Ligases / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster; D004797:Enzyme-Linked Immunosorbent Assay; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009699:N-Glycosyl Hydrolases / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D019895:Transposases / Q000235:genetics / Q000378:metabolism*; D051981:Uracil-DNA Glycosidase
D029721:Drosophila Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D010446:Peptide Fragments; D018809:Proliferating Cell Nuclear Antigen; C103336:dap protein, Drosophila; D019895:Transposases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D011088:DNA Ligases; D000072481:DNA Ligase ATP