Parallelism between width and asymmetry of peaks of rigid, spherical particles in capillary zone electrophoresis using polymer solutions.
Peak width and peak asymmetry of rigid spherical particles in the size range of 3-100 nm radius (R) were measured in capillary zone electrophoresis (CZE), using buffered uncross-linked polyacrylamide of Mr 5.0 X 10(6). Polymer concentration-dependent spreading of peak width and peak asymmetry were found to parallel one another. The parallelism holds whether the particle size is within the "small" (R < 20 nm) or "large" (R > 20 nm) size ranges previously found to differ in the mechanism of particle size dependent retardation of electrophoretic migration (S. P. Radko and A. Chrambach, Electrophoresis 1996, 17, 1094-1102). In application to the "small" particle size range, the parallelism between band width and band asymmetry can be qualitatively interpreted to be consistent with the Giddings-Weiss mechanism (G. H. Weiss et al., Electrophoresis 1996, 17, 1325-1332) of electrophoresis in polymer-containing media which postulates a dependence of band width and band asymmetry on the equilibrium between "stationary" and "mobile" states of the particle.
Section on Macromolecular Analysis, Laboratory of Cellular and Molecular Biophysics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
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Radko
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Chrambach
D000180:Acrylic Resins; D004058:Diffusion; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D011108:Polymers; D012709:Serum Albumin / Q000032:analysis; D012996:Solutions
D000180:Acrylic Resins; D011108:Polymers; D012709:Serum Albumin; D012996:Solutions; C016680:polyacrylamide gels