A missense mutation of the gene for Na+,K(+)-ATPase alpha-subunit causes abnormal feeding behavior in Caenorhabditis elegans.
The Na+,K(+)-ATPase activity of membranes from a behavioral mutant of C. elegans was found to be about one-third that of the wild-type. The levels of mRNA and polypeptide of Na+,K(+)-ATPase alpha-subunit in the mutant were as high as those in the wild-type, but the level of the phosphorylated intermediate of the Na+,K(+)-ATPase in the mutant worm was 80% lower than that in the wild-type. A single predicted amino acid replacement (Leu to Phe) was found in a highly conserved region in the alpha-subunit that is involved in the formation of phosphorylated intermediate. The abnormal feeding behavior of the mutant worm may be attributed to this missense mutation.
Laboratory of Biochemistry, Kyorin University School of Health Sciences, Tokyo, Japan.
Y
Y
Shima
Y
Y
Tada
M
M
Furuki
Y
Y
Hara
H
H
Ohta
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology; D017124:Conserved Sequence; D018509:DNA, Helminth / Q000235:genetics; D005247:Feeding Behavior; D017238:Genes, Helminth; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010042:Ouabain / Q000494:pharmacology; D010766:Phosphorylation; D017354:Point Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D018509:DNA, Helminth; D046911:Macromolecular Substances; D012333:RNA, Messenger; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase