Separation of basic drug substances of very similar structure using micellar electrokinetic chromatography.
Small molecules having very similar molecular structure--some even with the same mass over charge--are not trivial to separate by capillary electrophoresis in free solution. However, addition of surfactants to the electrophoretic buffer and thus using the principle of micellar electrokinetic chromatography may provide separation with high resolution. The use of zwitterionic and nonionic surfactants is demonstrated and an example of how a developed system may be used for drug purity testing is given.
Royal Danish School of Pharmacy, Department of Analytical and Pharmaceutical Chemistry, 2 Universitetsparken, Copenhagen.
S H
SH
Hansen
I
I
Bjørnsdottir
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J
Tjørnelund
D000588:Amines / Q000737:chemistry; D002845:Chromatography; D004563:Electrochemistry; D008823:Micelles; D008970:Molecular Weight; D004364:Pharmaceutical Preparations / Q000737:chemistry / Q000302:isolation & purification*; D013501:Surface-Active Agents / Q000737:chemistry
D000588:Amines; D008823:Micelles; D004364:Pharmaceutical Preparations; D013501:Surface-Active Agents