The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors.
The lack of a well-behaved permanent, adherent, nontransformed chicken cell line has made some experiments with avian leukosis-sarcoma viruses (ASLV) and vectors considerably more difficult. The EV-O-derived line, DF-1, supports the efficient replication of subgroups (A), (B), and (C) ASLV, as well as amphotrophic murine leukemia virus and an ASLV-derived vector that has its env gene derived from the env gene from an amphotrophic murine leukemia virus. The cell line responds appropriately to the expression of a transforming oncogene (v-myc) to a growth suppressor gene [p21(waf1)] and can be sorted (using FACS) if infected by an ASLV vector that expresses GFP.
Molecular Medicine Program, Mayo Clinic and Mayo Foundation, Rochester, Minnesota, 55905, USA.
J
J
Schaefer-Klein
I
I
Givol
E V
EV
Barsov
J M
JM
Whitcomb
M
M
VanBrocklin
D N
DN
Foster
M J
MJ
Federspiel
S H
SH
Hughes
D001355:Alpharetrovirus / Q000502:physiology*; D000818:Animals; D002460:Cell Line / Q000821:virology*; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000502:physiology; D016259:Genes, myc / Q000502:physiology; D005822:Genetic Vectors; D009052:Leukemia Virus, Murine / Q000502:physiology; D009857:Oncogenes / Q000502:physiology; D014759:Viral Envelope Proteins / Q000502:physiology; D014779:Virus Replication
D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D014759:Viral Envelope Proteins