Protective effects of interleukin-10 on diaphragm muscle in a septic animal model.
The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline + endotoxin group as control and an IL-10 + endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline + endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p < 0.001 and p < 0.05, respectively) compared to those at 0 hour. In the IL-10 + endotoxin group, the decrement in the force-frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline + endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-10 + endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.
First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
A
A
Taneda
C
C
Shindoh
Y
Y
Ohuchi
K
K
Shirato
D000818:Animals; D003964:Diaphragm / Q000187:drug effects; D004731:Endotoxins / Q000633:toxicity*; D004926:Escherichia coli; D006651:Histocytochemistry; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D016753:Interleukin-10 / Q000494:pharmacology*; D009119:Muscle Contraction / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D051381:Rats; D017208:Rats, Wistar
D004731:Endotoxins; D016753:Interleukin-10; D009569:Nitric Oxide; D009252:NADPH Dehydrogenase