Transformation of Penicillium griseoroseum nitrate reductase mutant with the nia gene from Fusarium oxysporum.
A heterologous transformation system for Penicillium griseoroseum has been developed. This system is based on nia, the structural gene from Fusarium oxysporum encoding nitrate reductase. Penicillium griseoroseum niaD mutants have been selected from chlorate-resistant colonies. Among 24 chlorate-resistant colonies analyzed, 2 were confirmed to be niaD mutants. Transformation frequencies of 8 transformants/microgram of DNA were obtained. DNA hybridization analyses of five transformants showed distinct integration patterns of the plasmid and in all of them the integration occurred at tandem arrays. The transformation system established in this work will be useful for genetic studies of the pectinolytic complex genes from P. griseoroseum.
Departamento de Microbiologia/BIOAGRO, Universidade Federal de Viçosa, MG, Brazil.
M V
MV
de Queiroz
A O
AO
Barros
E G
EG
de Barros
V
V
Guimarães
E F
EF
de Araújo
D002704:Chlorates / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005670:Fusarium / Q000201:enzymology / Q000235:genetics*; D005800:Genes, Fungal; D009154:Mutation; D050901:Nitrate Reductase; D009565:Nitrate Reductases / Q000235:genetics*; D010407:Penicillium / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D011096:Polygalacturonase / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
D002704:Chlorates; D009565:Nitrate Reductases; D050901:Nitrate Reductase; D011096:Polygalacturonase