Identification of Cyclospora cayetanensis in stool using different stains.
Cyclospora cayetanensis, a newly emerging coccidian protozoa is world-wide in distribution. In the present study, different concentrations and staining techniques were used for identification of Cyclospora. Formol-ether sedimentation and Sheather's sugar flotation were used as concentration techniques and the different stains used were: the modified Ziehl-Neelsen, Giemsa, safranin-methylene blue, modifications of trichrome stain, calcoflour white and finally phenol-auramine. The safranin stain was the best, as it stained all the oocysts of Cyclospora uniformly, besides being rapid and easily applicable in the laboratories. Phenol-auramine stained the oocysts well, where both the wall and internal contents fluoresced brightly. With the calcoflour white stain, only the wall of oocysts took that fluorescent stain. The modified Ziehl-Neelsen stained some of the oocysts well, yet great variability in the staining pattern was noticed. Cyclospora oocysts were not efficiently stained with either trichrome modifications or Giemsa stains.
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Department of Parasitology, Faculty of Medicine, University of Alexandria, Egypt.
D000818:Animals; D003048:Coccidiosis / Q000469:parasitology; D016783:Eucoccidiida / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D006801:Humans; D013194:Staining and Labeling / Q000379:methods