Role of potassium in human immunodeficiency virus production and cytopathic effects.
Acute infection of CD4+ lymphoid cells by human immunodeficiency virus type 1 (HIV-1) induces an increase in the intracellular concentration of potassium (K+). Media containing reduced or elevated concentrations of K+ were used to investigate the role of this ion in HIV-1 production and cytopathology. Incubation of CD4+ lymphoblastoid cells acutely infected by HIV-1 (strain LAI) in low K+ medium resulted in an approximately 50% decrease in HIV-1 production and markedly diminished HIV-1 induced cytopathic effects (CPE) relative to cells incubated in medium containing a normal K+ concentration (approximately 5 mM). Incubation of HIV-1 infected cells in media containing elevated concentrations of K+ medium. Cells mM) increased HIV-1 production by two- to fivefold over the amount produced in cells incubated in normal K+ medium. Cells incubated in high K+ media also displayed enhanced HIV-1-induced cytopathology. The decrease in HIV-1 production by low K+ medium and increase by high K+ media could be a accounted for by effects on HIV-1 reverse transcription. However, low K+ medium inhibited HIV-1 protein synthesis and high K+ media increased HIV-1 protein synthesis. These results suggest that the HIV-1-induced increase in intracellular is required for efficient viral replication and to induce cytopathology.
Choi
B
B
Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112, USA.
Gatti
P J
PJ
Haislip
A M
AM
Fermin
C D
CD
Garry
R F
RF
D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002460:Cell Line; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D015497:HIV-1 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D011188:Potassium / Q000502:physiology*; D011533:Proviruses / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000821:virology; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D003470:Culture Media; D004279:DNA, Viral; D014764:Viral Proteins; D011188:Potassium