Sterically stabilized phospholipids attenuate human neutrophils chemotaxis in vitro.
The purpose of this study was to determine whether sterically stabilized liposomes (SSL) and poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) attenuate polymorphonuclear neutrophils (PMNs) chemotaxis in vitro and, if so, whether incorporation of vasoactive intestinal peptide (VIP), a pleiotropic neuropeptide, on the surface of SSL amplifies SSL-induced responses. Using a modified blind-well chamber chemotaxis assay, we found that N-formyl-methionyl-leucyl-phenylalanine (FMLP; 0.1 microM) and zymosan opsonized with purified human complement (2 x 10(9) yeast wall particles/ml) elicit significant human PMNs chemotaxis (95+/-9 and 103+/-3 cells/high power field; p<0.05). These effects are significantly attenuated by SSL and PEG-DSPE (p<0.05). By contrast, aqueous VIP and VIP on SSL have no significant effects on FMLP- and zymosan-induced responses. We conclude that certain sterically stabilized liposomes and phospholipids attenuate human PMNs chemotaxis in vitro and that VIP does not modulate this response.
Department of Medicine, University of Illinois at Chicago, and West Side Department of Veterans Affairs Medical Center, 60612-7323, USA.
U
U
Hatipoglu
X
X
Gao
S
S
Verral
F
F
Séjourné
D
D
Pitrak
H
H
Alkan-Onyüksel
I
I
Rubinstein
D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003165:Complement System Proteins; D004355:Drug Stability; D006801:Humans; D008081:Liposomes; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009895:Opsonin Proteins; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000008:administration & dosage / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000008:administration & dosage; D015054:Zymosan / Q000494:pharmacology
D008081:Liposomes; D009895:Opsonin Proteins; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D014660:Vasoactive Intestinal Peptide; D011092:Polyethylene Glycols; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003165:Complement System Proteins; D015054:Zymosan