Newly developed initial-flow cell filtrometer and the comparison with viscometry and ektacytometry on erythrocyte deformability.
According to Hanss's initial-flow rate method, F. Liao designed a new cell filtrometer in the early 1990s. The parameters, cell initial transit time (Tc) and membrane clogging rate (CR), can be determined by a single measurement with timing accuracy of 1 ms. The new cell filtrometer has two valuable features: a button-pushing operation to control electromagnetic valves; and an autocirculation of fluid in the cell filtrometer resulting in the same volume of leading fluid. A series of comparisons of the new filtrometry with viscometry or ektacytometry shows that the instrument seems to be more sensitive in detecting subpopulations of glutaraldehyde-hardened cells and the shift of deformability for all the cells. The reproducibility of the filtrometer for different membranes made in China and the USA is reasonable (CV < 5% for Tc). Preliminary application of the filtrometer indicates that it might be useful for trial study and routine clinical application.
Peng
J
J
Pharmacology Department, Chinese Institute of Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, China.
Liao
F L
FL
Yin
X J
XJ
D000818:Animals; D001809:Blood Viscosity; D004907:Erythrocyte Deformability; D005434:Flow Cytometry; D018056:Hemorheology / Q000295:instrumentation*; D008297:Male; D051381:Rats; D017208:Rats, Wistar