[Preparation and properties of monoclonal antibodies to tick-borne encephalitis viral nonstructural proteins].
Hybridomas secreting monoclonal antibodies (Mab) to tick-borne encephalitis (TBE) virus are obtained. Immunodiffusion showed that 3 Mabs to TBE protein NS3 belong to class IgM and the rest to IgG1. Mabs to TBE protein NS1 were tested in hemagglutination inhibition, complement fixation, neutralization, and protection tests. Only 1 hybridoma produced Mab specific for protein NS1 of TBE strain Sofyin, the rest reacted with the common antigenic determinants of nonstructural TBE complex.
Matveeva
V A
VA
Dobrikova
E Iu
EIu
Tsekhanovskaia
N A
NA
Popova
R V
RV
Pressman
E K
EK
Karavanov
A S
AS
Matveev
L E
LE
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins / Q000276:immunology*; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D000911:Antibodies, Monoclonal; D020365:RNA Helicases; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C100315:NS1 protein, Flavivirus; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases