Centrifugal partition chromatographic reaction for the production of chiral amino acids.
The use of a centrifugal partition chromatographic reactor is investigated for the production of chiral amino acids from racemic mixtures. Chirally selective enzymatic hydrolysis of N-acetyl-L-methionine into acetic acid and L-methionine was carried out in the chromatographic reactor to demonstrate the concept of integrated reaction and separation in centrifugal partition chromatography (CPC). The products L-methionine and acetic acid, as well as the unconverted substrate, N-acetyl-D-methionine are obtained separately. An aqueous two-phase system, consisting of PEG 600, potassium phosphate and water was successfully applied as liquid-liquid two-phase system in CPC. A model is presented, which describes the reaction chromatograms on the basis of the independently measured partition and mass transfer coefficients of the individual (reacting) components. The model appears to be a valuable tool for optimizing the reaction-separation process.
Kluyver Institute for Biotechnology, Delft, Netherlands.
J L
JL
den Hollander
B I
BI
Stribos
M J
MJ
van Buel
K C
KC
Luyben
L A
LA
van der Wielen
D000596:Amino Acids / Q000302:isolation & purification*; D002498:Centrifugation; D002845:Chromatography / Q000379:methods*; D006868:Hydrolysis; D007700:Kinetics; D008715:Methionine / Q000031:analogs & derivatives / Q000737:chemistry
D000596:Amino Acids; C006379:N-acetylmethionine; D008715:Methionine