Anti-inflammatory effect of interleukin-10 in rabbit immune complex-induced colitis.
BACKGROUND
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that downregulates the secretion of pro-inflammatory cytokines and additionally induces the secretion of anti-inflammatory cytokines, thus possibly leading to reduction of chronic inflammation in inflammatory bowel disease. In this study we evaluated the anti-inflammatory effect of IL-10 in a model of acute colitis in rabbits.


METHODS
Colitis was induced by rectal instillation of formalin, 0.65%, followed by intravenous infusion of 0.85 ml heat-aggregated rabbit immunoglobulin. Rabbits were treated with an intravenous bolus of recombinant human IL-10 (SCH52000), 100 microg/kg (n=14) or 500 microg/kg (n=14), 1 h before induction of colitis (control group, n=12).


RESULTS
High-dose IL-10 improved macroscopic scores of inflammation and decreased tissue myeloperoxidase levels and leukotriene B4 levels in dialysate fluid. Thromboxane B2 and prostaglandin E2 levels remained unaffected.


CONCLUSIONS
IL-10 ameliorates acute colitis in this model. Consequently, this anti-inflammatory cytokine may have a role in the therapy of acute inflammatory bowel disease.
Dept. of Gastroenterology, Academic Medical Center, Amsterdam, The Netherlands.
T A
TA
Grool
H
H
van Dullemen
J
J
Meenan
F
F
Koster
F J
FJ
ten Kate
A
A
Lebeaut
G N
GN
Tytgat
S J
SJ
van Deventer
D000208:Acute Disease; D000818:Animals; D000936:Antigen-Antibody Complex; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004760:Enterocolitis / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D016753:Interleukin-10 / Q000494:pharmacology / Q000502:physiology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D007975:Leukotriene B4 / Q000032:analysis; D008297:Male; D011817:Rabbits; D011897:Random Allocation; D012016:Reference Values
D000936:Antigen-Antibody Complex; D016753:Interleukin-10; D007975:Leukotriene B4