Antibiotic therapy determines subcutaneous Escherichia coli abscess formation after CD18 inhibition in rabbits.
Monoclonal antibodies (MAbs) that interrupt polymorphonuclear neutrophil (PMN)-endothelial cell adhesion can ameliorate PMN-mediated injury, including burn-induced inflammatory injury, but can also impair PMN-mediated defense against bacterial infection. We report the effects of combined anti-adhesion and antibiotic therapy on local infectious sequelae after subcutaneous Escherichia coli inoculation in rabbits treated with anti-CD18 (60.3) or anti-P-selectin (PB1.3) MAb. Ampicillin or ceftriaxone were administered for 72 hours. PMN emigration was assessed at 24 hours and local infectious sequelae at 7 days. In ampicillin/60.3-treated rabbits, E. coli inoculation resulted in impaired PMN emigration and increased infectious complications, with abscesses forming at a 10,000-fold lower inoculation concentration compared with other MAb-antibiotic treatment groups. We conclude that (1) CD18, but not P-selectin blockade interferes with PMN emigration and host defense to subcutaneous E. coli, and (2) appropriate antibiotic therapy can prevent the local infectious events caused by CD18 inhibition.
Talbott
G A
GA
Department of Anesthesiology, University of Washington School of Medicine, Seattle, USA.
Sharar
S R
SR
Paulson
J C
JC
Harlan
J M
JM
Winn
R K
RK
D000038:Abscess / Q000188:drug therapy* / Q000276:immunology*; D000667:Ampicillin / Q000627:therapeutic use*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002056:Burns / Q000150:complications; D018821:CD18 Antigens / Q000276:immunology / Q000502:physiology*; D002443:Ceftriaxone / Q000627:therapeutic use*; D002465:Cell Movement / Q000502:physiology; D002511:Cephalosporins / Q000627:therapeutic use*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D019007:P-Selectin / Q000276:immunology / Q000502:physiology*; D010406:Penicillins / Q000627:therapeutic use*; D011817:Rabbits
D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D002511:Cephalosporins; D019007:P-Selectin; D010406:Penicillins; D002443:Ceftriaxone; D000667:Ampicillin