Phenotypic analysis of cellular immune response in Swiss albino mice immunized with antigen extract from exposed B. alexandrina subjected to UV-, gamma irradiation and praziquantel treatment.
Mice were immunized (2x) subcutaneously at zero and 21st day with different antigen preparations. Splenocytes and mesenteric lymph nodes (MLN) cells were obtained from both immunized and control groups at 14th 21st and 42nd day post the 2nd immunization. The direct immunofluorescent assay was applied using anti mouse CD3+, D4+, CD8+, and IgG monoclonal antibodies to detect panel T-cells, T-cell subsets and B-cells respectively. The observations revealed that the splenic CD3+ T-cells did not show any considerable interrelation either between experimental groups or different time intervals post-immunization (P.I.). Meanwhile, UV-irradiated and PZQ-treated groups showed slight increase at the 2nd and 4th week P.I. as compared with the control group. The UV- and gamma-irradiated groups showed a significant elevation in CD4+ T-cells when compared with control group throughout the time of experiment, whereas, PZQ-treated group showed a significant increase at the 2nd and 4th week P.I. only. The frequency of CD8+ T-cells was elevated in all the experimental groups as compared with control group at 2nd, 4th and 6th week P.I. The MLN CD3+, CD4+ and CD8+ T-cells did not reveal any considerable changes between the experimental groups and control throughout the time of the experiment. A gradual decrease was observed in all the experimental groups by proceeding the time of the experiment. Splenic and MLN B-cells did not show considerable changes between the experimental groups and control group during the course of the experiment. It is concluded that these antigens could possibly stimulate cellular immune response upon their use as a protective antigens.
National Research Center, Dokki, Egypt.
A S
AS
Maghraby
H S
HS
Gad
D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology*; D001700:Biomphalaria / Q000187:drug effects / Q000276:immunology / Q000469:parasitology* / Q000528:radiation effects; D005720:Gamma Rays; D007114:Immunization; D016130:Immunophenotyping; D016131:Lymphocyte Subsets / Q000276:immunology*; D051379:Mice; D011223:Praziquantel / Q000494:pharmacology; D012550:Schistosoma mansoni / Q000187:drug effects / Q000276:immunology* / Q000528:radiation effects; D012555:Schistosomiasis mansoni / Q000517:prevention & control; D014466:Ultraviolet Rays
D000947:Antigens, Helminth; D011223:Praziquantel