The immunomodulating glycoprotein extract from Klebsiella pneumoniae RU 41740 exerts a suppressive effect on human monocyte death by apoptosis.
Programmed cell death or apoptosis is a physiological cell suicide process that can be suppressed by survival factors. Monocytes undergo rapid apoptosis in culture, unless signalled by cytokines or the bacterial lipopolysaccharide LPS. We have investigated the effect on monocyte apoptosis of the immunostimulating agent RU 41740 (Biostim), a glycoprotein extract from the Klebsiella pneumoniae K2O1 strain that is used for the prevention of recurrent infections. RU 41740, as LPS, strongly enhanced monocyte survival in vitro, an effect related to apoptosis suppression. RU 41740 at concentration ranging from 1 ng/ml to 10 microg/ml prevented apoptosis induced both by survival factor deprival and by gamma-irradiation. Our observation suggests that enhancement of monocyte survival may represent a component of the reported immunostimulating effect of this compound.
INSERM U415, Institut Pasteur, Lille, France.
J
J
Estaquier
C
C
Bloy
F
F
Corallo
C
C
Peron
J C
JC
Ameisen
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001426:Bacterial Proteins / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D008928:Mitochondria / Q000187:drug effects; D009000:Monocytes / Q000187:drug effects* / Q000502:physiology
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; C037567:glycoprotein, Klebsiella pneumoniae