Deferoxamine blocks interactions of fluoride and carbachol in isolated mammalian cardiac preparations.
In papillary muscles, carbachol reduced the positive inotropic effects of isoprenaline (10 nmol/l). The negative inotropic effects of carbachol in isoprenaline-stimulated guinea pig papillary muscles were attenuated by additionally applied sodium fluoride (3 mmol/l). These effects of sodium fluoride were blocked by deferoxamine (200 micromol/l). In guinea pig left atria, sodium fluoride alone greatly reduced force of contraction. These effects in atria were blocked by 200 micromol/l deferoxamine, and positive inotropic effects of sodium fluoride were observed. It is suggested that the cardiac effects of muscarinic M2 receptor agonists in the ventricle involve, at least in part, the activation of phosphatases which are blocked by fluoride and reactivated by deferoxamine.
Abteilung Allgemeine Pharmakologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
J
J
Neumann
H
H
Scholz
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D002217:Carbachol / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D003676:Deferoxamine / Q000494:pharmacology*; D005459:Fluorides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D006168:Guinea Pigs; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007502:Iron Chelating Agents / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D010210:Papillary Muscles / Q000187:drug effects / Q000201:enzymology; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D051381:Rats
D000318:Adrenergic beta-Agonists; D007502:Iron Chelating Agents; D018721:Muscarinic Agonists; D002217:Carbachol; D010749:Phosphoprotein Phosphatases; D003676:Deferoxamine; D007545:Isoproterenol; D005459:Fluorides