Enhancement of nitroxide-reducing activity in rats after chronic administration of vitamin E, vitamin C, and idebenone examined by an in vivo electron spin resonance technique.
Rats were given vitamin E (Vit-E), idebenone (ID), or vitamin C (Vit-C) in their food for 2 or 4 weeks. After feeding, the ability of rats to reduce 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (Tempol) in terms of the half-life of Tempol was examined as a specific marker. Tempol was repeatedly injected intravenously, and its half-life was serially evaluated by an in vivo electron spin resonance (ESR) technique. The radical-reducing ability in rats was enhanced differently by Vit-E, ID, and Vit-C, i.e., slow onset of the ability after Vit-E and ID (lipid-soluble antioxidants) and fast onset after Vit-C (a water-soluble antioxidant).
Department of Neuropsychiatry, Fukushima Medical College, Japan.
S
S
Matsumoto
N
N
Mori
N
N
Tsuchihashi
T
T
Ogata
Y
Y
Lin
H
H
Yokoyama
S
S
Ishida
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D016227:Benzoquinones / Q000494:pharmacology*; D003497:Cyclic N-Oxides / Q000493:pharmacokinetics*; D004578:Electron Spin Resonance Spectroscopy; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013113:Spin Labels; D014451:Ubiquinone / Q000031:analogs & derivatives; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D016227:Benzoquinones; D003497:Cyclic N-Oxides; D013113:Spin Labels; D014451:Ubiquinone; D014810:Vitamin E; C036619:idebenone; D001205:Ascorbic Acid; C001803:tempol