C-terminal truncation of the Sup35 protein increases the frequency of de novo generation of a prion-based [PSI+] determinant in Saccharomyces cerevisiae.
The yeast non-Mendelian [PSI+] determinant is presumed to be the manifestation of the aggregated prion-like state of the Sup35 protein. Plasmid-mediated amplification of the SUP35 gene greatly increases the frequency of Sup35p transition to this prion-like state. Here we show that the 3'-deletions of plasmid SUP35, leading to the C-terminal truncation of Sup35p, further increase the frequency of [PSI+] induction despite a marked decrease in Sup35p expression levels. The data suggest that the presence of Sup35p N-terminal proteolytic fragments can cause [PSI+] appearance in wild-type yeast cells.
Kochneva-Pervukhova
N V
NV
Institute of Experimental Cardiology, Cardiology Research Center, 121552 Moscow, Russia. vita@excard.msk.su
Poznyakovski
A I
AI
Smirnov
V N
VN
Ter-Avanesyan
M D
MD
D000483:Alleles; D005656:Fungal Proteins / Q000235:genetics*; D005784:Gene Amplification; D015870:Gene Expression; D005800:Genes, Fungal; D010454:Peptide Termination Factors; D010957:Plasmids / Q000235:genetics; D011328:Prions / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017384:Sequence Deletion; D014170:Transformation, Genetic
D005656:Fungal Proteins; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins