A relatively small change in sodium chloride concentration has a strong effect on adhesion of ocular bacteria to contact lenses.
Adhesion of bacteria to hydrogel lenses is thought to be an initial step of ocular colonization allowing evasion of normal host defences. The salt concentration of media is an important parameter controlling microbial adhesion. Salinity varies from 0.97% NaCl equivalents in the open eye to 0.89% in the closed eye state. In this study, the effect of sodium chloride in the concentration range of 0.8-1.0% (w/v) NaCl on adhesion of ocular bacteria to soft contact lenses was investigated using a static adhesion assay. Pseudomonas aeruginosa was found to adhere to lenses in significantly greater amounts than Serratia marcescens, Flavobacterium meningosepticum, Stenotrophomonas maltophilia and Staphylococcus intermedius. Increasing NaCl from 0.8% to 1.0% (w/v) increased adhesion of all bacteria tested. This adhesion was strong since the organisms could not be removed by washing in low ionic buffer. Adhesion of these organisms did not correlate with their cell surface properties as determined by bacterial adhesion to hydrocarbons (BATH) and retention on sepharose columns.
Cowell
B A
BA
Co-operative Research Centre for Eye Research and Technology, University of New South Wales, Sydney, Australia.
Willcox
M D
MD
Schneider
R P
RP
D001422:Bacterial Adhesion / Q000187:drug effects*; D002847:Chromatography, Agarose; D003263:Contact Lenses, Hydrophilic; D006090:Gram-Negative Bacteria / Q000502:physiology*; D006801:Humans; D006838:Hydrocarbons; D012965:Sodium Chloride / Q000494:pharmacology*; D013210:Staphylococcus / Q000502:physiology; D013499:Surface Properties; D013666:Tears / Q000737:chemistry
D006838:Hydrocarbons; D012965:Sodium Chloride