Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media.
Lactobacillus rhamnosus GG and Lactobacillus rhamnosus LC-705, previously shown to effectively bind to aflatoxin B1, were subjected to various chemical and physical treatments to examine the effects of these treatments on the binding affinity of these strains toward aflatoxin B1. Treatment of bacterial pellets of both strains with hydrochloric acid significantly (P < 0.05) enhanced the binding ability to bind aflatoxin B1 was also observed when the bacterial pellets were subjected to heat treatment by either autoclaving or boiling at 100 degrees C in a water bath, put the impact of these two treatments was not as effective as the acid treatment. Ethanol, UV radiation, sonication, alkaline, or pH treatments either had not effect or reduced the binding ability of the bacteria.
Key Centre for Applied Nutritional Toxicology, RMIT-University, Melbourne, Vic, Australia.
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el-Nezami
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Kankaanpää
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Salminen
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Ahokas
D019840:2-Propanol / Q000494:pharmacology; D000348:Aflatoxins / Q000378:metabolism*; D003470:Culture Media; D006358:Hot Temperature; D006851:Hydrochloric Acid / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013010:Sonication
D000348:Aflatoxins; D003470:Culture Media; D012972:Sodium Hydroxide; D011189:Potassium Chloride; D017693:Sodium Bicarbonate; D019840:2-Propanol; D006851:Hydrochloric Acid