Effects of capsaicin and capsaicin desensitization on alanine accumulation in rat hemidiaphragms.
1. Alanine accumulation in rat hemidiaphragms is significantly inhibited by capsaicin in a dose-dependent pattern that produced maximal effect at 1 mM. 2. The inhibitory effect of capsaicin on alanine accumulation by rat hemidiaphragms was enhanced upon preincubation with 5 mM ouabain. However, preincubation with 1 mM Verapamil did not alter the inhibitory effect of capsaicin. 3. Chemical ablation of the capsaicin-sensitive primary afferent fibers abolished the inhibitory effect of capsaicin on alanine accumulation in the rat hemidiaphragms.
Department of Physiology, American University of Beirut, Lebanon.
N A
NA
Kanj
M
M
Samaha
C F
CF
Nassar
D000409:Alanine / Q000378:metabolism*; D000818:Animals; D001692:Biological Transport; D002211:Capsaicin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003964:Diaphragm / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014700:Verapamil / Q000494:pharmacology
D010042:Ouabain; D014700:Verapamil; D000409:Alanine; D002211:Capsaicin