The quality control of glycoprotein folding in the endoplasmic reticulum, a trip from trypanosomes to mammals.
The present review deals with the stages of synthesis and processing of asparagine-linked oligosaccharides occurring in the lumen of the endoplasmic reticulum and their relationship to the acquisition by glycoproteins of their proper tertiary structures. Special emphasis is placed on reactions taking place in trypanosomatid protozoa since their study has allowed the detection of the transient glucosylation of glycoproteins catalyzed by UDP-Glc:glycoprotein glucosyltransferase and glucosidase II. The former enzyme has the unique property of covalently tagging improperly folded conformations by catalyzing the formation of protein-linked Glc1Man7GlcNAc2, Glc1Man8GlcNac2 and Glc1Man9GlcNAc2 from the unglucosylated proteins. Glucosyl-transferase is a soluble protein of the endoplasmic reticulum that recognizes protein domains exposed in denatured but not in native conformations (probably hydrophobic amino acids) and the innermost N-acetylglucosamine unit that is hidden from macromolecular probes in most native glycoproteins. In vivo, the glucose units are removed by glucosidase II. The influence of oligosaccharides in glycoprotein folding is reviewed as well as the participation of endoplasmic reticulum chaperones (calnexin and calreticulin) that recognize monoglucosylated species in the same process. A model for the quality control of glycoprotein folding in the endoplasmic reticulum, i.e., the mechanism by which cells recognize the tertiary structure of glycoproteins and only allow transit to the Golgi apparatus of properly folded species, is discussed. The main elements of this control are calnexin and calreticulin as retaining components, the UDP-Glc:glycoprotein glucosyltransferase as a sensor of tertiary structures and glucosidase II as the releasing agent.
Parodi
A J
AJ
Instituto de Investigaciones Bioquímicas, Fundación Campomar, Buenos Aires, Argentina. aparodi@iris.iib.uba.ar
D000818:Animals; D001216:Asparagine / Q000378:metabolism; D004721:Endoplasmic Reticulum; D005964:Glucosyltransferases; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D008322:Mammals; D009844:Oligosaccharides; D017510:Protein Folding; D014351:Trypanosomatina
D006023:Glycoproteins; D009844:Oligosaccharides; D001216:Asparagine; D005964:Glucosyltransferases