An RNA oligonucleotide corresponding to the polypyrimidine region of the rat alpha 1(I) procollagen promoter forms a stable triplex and inhibits transcription.
In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat alpha 1(I) procollagen promoter with a Kd of 0.1-0.2 microM. The formation of triplexes by the triplex forming oligoribonucleotide (ORN) was also observed under physiological conditions. In vitro transcription run-off experiments showed that triplex formation results in inhibited transcription from the rat alpha 1(I) procollagen gene. Our results demonstrate a novel approach for down-regulation of procollagen gene transcription in vivo.
Ririe
S S
SS
Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri-Columbia 65212, USA.
Guntaka
R V
RV
D000818:Animals; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000235:genetics* / Q000494:pharmacology; D011347:Procollagen / Q000737:chemistry / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011743:Pyrimidines / Q000737:chemistry; D051381:Rats; D014158:Transcription, Genetic / Q000187:drug effects; D015533:Transcriptional Activation
D009841:Oligonucleotides; D011347:Procollagen; D011743:Pyrimidines