High-performance liquid chromatography with a column-switching system and capillary electrophoresis for the determination of ibuprofen in plasma.
Quantitative aspects of high-performance liquid chromatography with a column-switching system (CSS-HPLC) and capillary electrophoresis (CE) were investigated for the determination of ibuprofen in plasma. For CSS-HPLC, 100 microl of plasma was directly injected onto the column system for the three separation steps: (1) deproteinization and fractionation of plasma samples with a polymer-coated mixed-function phase column, (2) concentration with an intermediate column and (3) final separation with a main column. For CE, a mixture of 50 microl of plasma and 1 ml of acetonitrile was centrifuged and the supernatant was introduced onto the capillary (66 cmX50 microm I.D.; 62 cm to detector) at 20 degrees C. Run buffer was 250 mM sodium borate buffer (pH 8.5) and applied electric field was 379 V cm(-1). Linear dynamic ranges were 0.1-250 microg ml(-1) in CSS-HPLC and 1-1000 microg ml(-1) in CE. Intra-day and inter-day coefficients of variation were less than 5.6% and 6.5% for CSS-HPLC, 6.3% and 6.5% for CE, respectively. The limits of detection (S/N=3) for CSS-HPLC and CE were 25 ng ml(-1) and 300 ng ml(-1), respectively. CSS-HPLC was superior in simplicity and sensitivity, while CE was better in efficiency, rapidity, and cost.
Department of Chemistry, Seoul National University, South Korea.
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D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D007052:Ibuprofen / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007052:Ibuprofen