Validation of predictive equations for use of deuterium oxide dilution to determine body composition of dogs.
OBJECTIVE
To validate equations predicting body composition of dogs, using deuterium oxide dilution.


METHODS
38 female and 37 male dogs selected by defined body weight and body condition criteria.


METHODS
Measured equilibrated deuterium concentration in serum after i.v. administration of isotope was used to determine isotope space and predict body water, fat, nitrogen, and ash contents determined from analysis of homogenized carcass. Equations predicting body composition were derived, using regression analysis, and were validated, using data-splitting techniques.


RESULTS
Deuterium space (in kilograms) overestimated kilograms of body water content by mean 21.8% +/- SD of 4.18%. Regression equations were derived and validated to predict kilograms of body water and ash from kilograms of deuterium space, and proportion of body water, fat, nitrogen, and ash from proportion of deuterium space. Coefficients of determination (r2) and means of standard errors of estimating new values (SEE) were, respectively, 0.993 and 0.4 kg for body water content, and 0.942 and 0.08 kg for ash content. For proportions, SEE were 2.0, 2.7, 0.1, and 0.4% for body water, fat, nitrogen, and ash, respectively. Two factors, time for isotope equilibration and whether samples were processed by vacuum sublimation, improved SEE for proportion of body water from 2.0 to 1.3% and of fat from 2.7 to 1.8%. Equations predicting absolute quantities of fat and nitrogen could be derived but not validated.


CONCLUSIONS
Deuterium dilution can be used to predict body composition of dogs with precision equal to that determined for other species.
Burkholder
W J
WJ
Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg 24061-0442, USA.
Thatcher
C D
CD
D000818:Animals; D001823:Body Composition; D001834:Body Water; D001835:Body Weight; D017666:Deuterium Oxide / Q000493:pharmacokinetics*; D004285:Dogs / Q000033:anatomy & histology*; D005260:Female; D008297:Male; D011865:Radioisotope Dilution Technique / Q000662:veterinary; D012044:Regression Analysis; D015203:Reproducibility of Results
D017666:Deuterium Oxide