A genome-derived (gaa.ttc)24 trinucleotide block binds nuclear protein(s) specifically and forms triple helices.
The properties of simple trinucleotide repeats generate increased interest as expansions of certain trinucleotide blocks cause human diseases. Here, we studied protein binding and structural features of a perfect (gaa.ttc)24 tract in its original genomic environment. Electrophoretic mobility shift assays revealed that HeLa nuclear proteins bind to the DNA fragment containing the (gaa.ttc)24 block. Competition experiments using simple (gt.ac)n repeats differing in length and flanking regions showed no cross-reactivity with the major retarded band. For the specific (gaa. ttc)n/protein complex, a binding constant of 9.3x10-9 mol/l was determined. DNase I footprinting revealed protein binding sites located exclusively within the repeat with a preference for the (gaa)24 strand. OsO4 and DEPC modifications followed by electrophoretic and electron microscopical analyses showed that the (gaa.ttc)24 block forms different types of intramolecular triple helices: Under superhelical stress, different H-DNA isomers are evident, whereas exclusively H-Y forms were detected in the relaxed state. Together, these data have functional implications for genomic (gaa.ttc)n tracts.
Mäueler
W
W
Department of Molecular Human Genetics, Ruhr University, 44780, Bochum, Germany.
Kyas
A
A
Keyl
H G
HG
Epplen
J T
JT
D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D018983:DNA Footprinting; D004278:DNA, Superhelical / Q000378:metabolism / Q000648:ultrastructure; D003850:Deoxyribonuclease I; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008854:Microscopy, Electron; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism*; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry* / Q000378:metabolism; D009993:Osmium Tetroxide / Q000494:pharmacology; D010957:Plasmids / Q000378:metabolism / Q000648:ultrastructure; D018911:Trinucleotide Repeats
D004278:DNA, Superhelical; D009687:Nuclear Proteins; D009838:Oligodeoxyribonucleotides; D003850:Deoxyribonuclease I; D004047:Diethyl Pyrocarbonate; D009993:Osmium Tetroxide