Amplification of TCRbeta gene rearrangements from micromanipulated single cells: T cells rosetting around Hodgkin and Reed-Sternberg cells in Hodgkin's disease are polyclonal.
Despite accounting for only a minor fraction of all cells in Hodgkin's lymphoma tissue, the Hodgkin and Reed-Sternberg (HRS) cells represent the malignant tumor cell clone in Hodgkin's disease (HD). By far the most abundant cell type in the tumor tissue are CD4+ T cells. Some of them intimately associate with HRS cells forming rosettes around them. This study addresses the question whether the rosetting phenomenon reflects a specific interaction between T and HRS cells by asking whether the rosettes are composed of T cells expressing a restricted TCR repertoire. Single rosetting T cells were micromanipulated from frozen sections of tumor tissue in two cases of nodular sclerosing HD and one case of lymphocyte predominant HD. TCR Vbeta gene rearrangements were amplified from these single cells by PCR. Of 83 potentially functional Vbeta gene rearrangements obtained altogether from the three cases, 81 were found to be clonally unrelated. Furthermore, they did not show signs of selection of the receptor chains for recognition of common epitopes: The usage of Vbeta and Jbeta gene segments as well as the distribution of complementarity-determining region (CDR) 3 lengths was similar to what was seen in a collection of 60 Vbeta gene rearrangements from blood of healthy donors and no recurrent CDR3 amino acid motifs were found. These data suggest that the HRS cells attract CD4+ T cells nonspecifically.
Institute for Genetics, University of Cologne, Germany. axel.roers@uni-koeln.de
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A
Roers
M
M
Montesinos-Rongen
M L
ML
Hansmann
K
K
Rajewsky
R
R
Küppers
D000328:Adult; D001483:Base Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000473:pathology; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005784:Gene Amplification; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006689:Hodgkin Disease / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016539:Reed-Sternberg Cells / Q000276:immunology* / Q000473:pathology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology* / Q000473:pathology*
D017931:DNA Primers