In vivo transduction with shiga toxin 1-encoding phage.
To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.
Division of Geographic Medicine and Infectious Diseases and Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts, USA.
D W
DW
Acheson
J
J
Reidl
X
X
Zhang
G T
GT
Keusch
J J
JJ
Mekalanos
M K
MK
Waldor
D000668:Ampicillin Resistance; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D007422:Intestines; D051379:Mice; D016254:Mutagenesis, Insertional; D022622:Shiga Toxin 1; D014161:Transduction, Genetic
D001427:Bacterial Toxins; D022622:Shiga Toxin 1