cDNA cloning and chromosomal localization of the human and mouse isoforms of Ksp-cadherin.
Ksp-cadherin is a novel kidney-specific member of the cadherin superfamily of cell adhesion molecules. We have determined the complete cDNA coding sequences of both the human and the mouse isoforms of Ksp-cadherin. The inferred amino acid sequences of the human and mouse isoforms are 79 and 75% identical to the originally described rabbit isoform of Ksp-cadherin (Thomson et al., 1995; J. Biol. Chem. 270, 17594-17601), respectively. The relative locations of cadherin-specific sequence motifs, putative N-glycosylation sites, and characteristic protein domains are entirely conserved in all three isoforms. Multiple organ Northern analyses indicate that, as in the rabbit, both the human and the mouse Ksp-cadherin transcripts appear to have distinct kidney-specific distributions. The human Ksp-cadherin gene (CDH16) maps to chromosome 16q21-proximal 16q22. The mouse Ksp-cadherin gene (Cdh16) was localized to a highly syntenic region of distal Chromosome 8. Both the human and the mouse Ksp-cadherin genes were localized to previously identified clusters of cadherin gene sequences, consistent with the hypothesis that most cadherin family members arose by gene duplication from a single ancestral gene at a relatively early stage in the evolution of the mammalian genome.
Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut, 06520-8029, USA. thomson@biomed.med.yale.edu
R B
RB
Thomson
D C
DC
Ward
S E
SE
Quaggin
P
P
Igarashi
Z E
ZE
Muckler
P S
PS
Aronson
D000595:Amino Acid Sequence; D000818:Animals; D015820:Cadherins / Q000737:chemistry*; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D002874:Chromosome Mapping; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics; D003001:Cloning, Molecular; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007668:Kidney / Q000502:physiology*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
C114483:CDH16 protein, human; D015820:Cadherins; C491946:Cdh16 protein, mouse; D015815:Cell Adhesion Molecules; D012333:RNA, Messenger