[Detection of chromosome translocation t(9;22) using RT-PCR].
The investigation deals with a simplified modification or molecular-genetic detection of translocation t(9;22) using a combination of reverse transcription and polymerase chain reactions (RT-PCR). Unlike the available protocols, analysis is carried out using one enzyme--TET-Z polymerase--(instead of two) which has both revertase and DNA-polymerase activities. The present modification is highly sensitive, less time-consuming and cheaper. The method has proved useful for both diagnosing t(9;22) translocation and diagnosing and monitoring minimal residual disease remaining after marrow transplantation.
Russian Research Institute of Hematology and Transfusiology, Acad. I.P.Pavlov State Medical University, St.Petersburg.
Zh IU
ZhI
Sidorova
K V
KV
Bogdanov
S I
SI
Kapustin
A A
AA
Lyshchev
O V
OV
Marinets
Iu S
IuS
Ogorodnikova
I S
IS
Martynkevich
K O
KO
Sitskaia
L S
LS
Zubarovskaia
A Iu
AIu
Zaritskiĭ
B V
BV
Afanas'ev
K M
KM
Abdulkadyrov
M N
MN
Blinov
D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D006801:Humans; D018365:Neoplasm, Residual / Q000175:diagnosis* / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012194:RNA-Directed DNA Polymerase; D014178:Translocation, Genetic
D012194:RNA-Directed DNA Polymerase