Activation of cAMP synthesis in rat brain cortical membranes by rubidium and cesium ions.
Rubidium and cesium chlorides accelerated cAMP synthesis in rat brain cortex membranes, while other alkali metal chlorides had no influence on the rate of this process. The effect was dose-dependent and yielded above 2-fold activation of adenylate cyclase. It has been shown that Rb+ and Cs+ influenced directly the catalytic subunit of the enzyme and did not substitute Mg2+ in formation of the metal-ATP complex in this reaction. The stimulatory effect of Rb+ was additive to the activation of adenylate cyclase by the half-maximal (0.3 microM) as well as by the saturating (10 microM) forskolin concentrations, pointing to the fact that these effectors activate different isoforms of the enzyme in rat brain cortex.
Institute of Chemical Physics, University of Tartu, Estonia.
K
K
Rosenthal
J
J
Lember
E
E
Karelson
J
J
Järv
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D002586:Cesium / Q000494:pharmacology*; D002712:Chlorides / Q000494:pharmacology*; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006160:Guanosine Triphosphate / Q000494:pharmacology; D066298:In Vitro Techniques; D015636:Magnesium Chloride / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D012413:Rubidium / Q000494:pharmacology*
D002712:Chlorides; D015636:Magnesium Chloride; D005576:Colforsin; D002586:Cesium; D006160:Guanosine Triphosphate; D000242:Cyclic AMP; C028019:cesium chloride; D012413:Rubidium; C032710:rubidium chloride