Simplified method of identifying severe combined immunodeficient (SCID) mice versus non-SCID mice by flow cytometric analysis of peripheral blood.
Several studies have utilized simple breeding strategies to create new immunodeficient mouse strains from severe combined immunodeficient (SCID) mice and non-SCID mice with secondary traits in order to evaluate the involvement of lymphocytes and immune responses in a variety of processes. We utilized a breeding strategy with C.B-17scid/scid (SCID) (H-2d) mice and SJL (H-2s) mice to generate immunodeficient mice that were histocompatible with the inbred SJL strain (H-2s) in order to evaluate the role of histocompatible recipient lymphocytes in adoptively transferred autoimmune disease mediated by SJL T lymphocytes. [SCID x SJL]F1 mice (heterozygous for H-2 loci and heterozygous for the SCID mutation) were backcrossed with SCID mice and the resulting offspring expressed a variety of phenotypes, including SCID or non-SCID and H-2s/H-2d or H-2d/H-2d. In order to screen offspring for the desired phenotype (SCID, H-2s), a flow cytometric method utilizing forward- and side-scatter parameters of peripheral blood cells was used to distinguish SCID from non-SCID animals. This method simplified the screening process and was as reliable as anti-CD3 fluorescent monoclonal antibody staining for detecting the presence (non-SCID) or absence (SCID) of T lymphocytes in peripheral blood.
Research Service, VA Medical Center, Portland, Oregon 97201, USA.
S
S
Murray
S
S
Subramanian
D N
DN
Bourdette
R E
RE
Jones
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D017252:CD3 Complex / Q000032:analysis; D003433:Crosses, Genetic; D005260:Female; D005434:Flow Cytometry / Q000379:methods*; D019085:Fluorescent Antibody Technique, Direct; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D010641:Phenotype; D016511:Severe Combined Immunodeficiency / Q000175:diagnosis*
D000911:Antibodies, Monoclonal; D017252:CD3 Complex