Humoral immune response to mycobacterial heat shock protein (hsp)65 in the cerebrospinal fluid of neuro-Behçet patients.
Although systemic immune reactivity to 65-kD mycobacterial hsp65 (m-hsp65) has been shown previously in Behçet's disease (BD), local immune response was not investigated. We studied anti-m-hsp65 IgG, IgM and IgA antibodies in the serum and cerebrospinal fluid (CSF) of 25 BD patients with cerebral parenchymal involvement (p-NBD), seven BD patients with intracranial hypertension (ih-NBD), eight BD patients without central nervous system (CNS) involvement, 30 patients with multiple sclerosis (MS) and 24 patients with non-inflammatory CNS disorders (NIC). Significantly higher CSF IgG responses were detected in p-NBD patients (ELISA ratio 1.3 +/- 0.9) compared with NIC (0.7 +/- 0.4, P < 0.01). In p-NBD patients' IgG, IgM or IgA CSF anti-m-hsp65 positivity rate was 48% (12/25); this was significantly higher when compared with MS (3/30; P < 0.03) and NIC (3/24; P < 0.01). CSF anti-m-hsp65 IgG ratios correlated with the duration of BD (r = 0.4, P < 0.04) but not with the duration of neurological involvement. Serum IgM and IgA responses were elevated in ih-NBD, suggesting a different type of involvement than p-NBD. These results implicate an increased local humoral response to m-hsp65 in the CSF of p-NBD patients, which might be related to the pathogenesis of neurological involvement.
Taşçi
B
B
Department of Neurology, Medical Faculty of Istanbul, Turkey.
Direskeneli
H
H
Serdaroglu
P
P
Akman-Demir
G
G
Eraksoy
M
M
Saruhan-Direskeneli
G
G
D000328:Adult; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000134:cerebrospinal fluid* / Q000276:immunology*; D001426:Bacterial Proteins; D001528:Behcet Syndrome / Q000134:cerebrospinal fluid* / Q000276:immunology*; D018834:Chaperonin 60; D018833:Chaperonins / Q000134:cerebrospinal fluid* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D007070:Immunoglobulin A; D007074:Immunoglobulin G; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins