Screening of chlorpropamide in horse plasma by high-performance liquid chromatography with ultraviolet absorbance detection, and confirmation by gas chromatography-mass spectrometry.
A chromatographic method was developed to detect and confirm the presence of chlorpropamide (I) in horse plasma samples, for antidoping control. The plasma sample (1 ml) was extracted with dichloromethane and screened by high-performance liquid chromatography, and confirmation of the drug's presence was accomplished by using gas chromatography-mass spectrometry (GC-MS). The limit of detection was found to be 3.5 ng/ml at a signal-to-noise ratio of three. Derivatization of I with N,O-bis-(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane allowed for highly stable, accurate and sensitive GC-MS analysis. Plasma samples collected after the administration of diabinese were positive for I (one-five days) in all samples analysed.
Chua
H C
HC
Malayan Racing Association, Bukit Timah Racecourse, Singapore, Singapore.
Stewart
B
B
Lim
B H
BH
Lee
H K
HK
D000818:Animals; D002747:Chlorpropamide / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D004300:Doping in Sports; D008401:Gas Chromatography-Mass Spectrometry; D006736:Horses / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D015813:Substance Abuse Detection / Q000379:methods*
D007004:Hypoglycemic Agents; D002747:Chlorpropamide