A fluorescence-based method for assessing dural protein extravasation induced by trigeminal ganglion stimulation.
Neurogenic dural inflammation has been proposed as a source of pain during migraine. Unilateral electrical stimulation of the trigeminal ganglion causes the ipsilateral release of inflammatory neuropeptides and subsequent dural plasma protein extravasation, a component of neurogenic inflammation. We measured the amount of protein leaking into the dural tissue of guinea pigs following trigeminal ganglion stimulation by exploiting the complexation reaction of endogenous proteins with the fluorescent dye Evans Blue, instead of utilizing exogenous radiolabeled albumin as commonly done in the literature. The amount of Evans Blue trapped in dural tissue following electrical stimulation of the trigeminal ganglion was measured using a fluorescence microscope equipped with a spectrophotometer. This method utilized multiple measurements on each dura sample which resulted in very precise values using a small number of animals per point (n = 3). Sumatriptan and CP-122,288 were found to dose-dependently prevent neurogenic dural extravasation. The potencies of CP-122,288 and sumatriptan were found to be similar to those reported in the literature when similar experimental protocols were used.
Johnson
K W
KW
Central Nervous System Research, Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN 46285, USA.
Phebus
L A
LA
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004388:Dura Mater / Q000378:metabolism*; D004558:Electric Stimulation; D005070:Evans Blue / Q000008:administration & dosage; D005119:Extravasation of Diagnostic and Therapeutic Materials / Q000378:metabolism*; D005453:Fluorescence; D006168:Guinea Pigs; D007249:Inflammation / Q000209:etiology / Q000378:metabolism; D007275:Injections, Intravenous; D008297:Male; D008856:Microscopy, Fluorescence; D015591:Microspectrophotometry; D008881:Migraine Disorders / Q000209:etiology; D011485:Protein Binding; D011506:Proteins / Q000032:analysis* / Q000378:metabolism*; D011759:Pyrrolidines / Q000008:administration & dosage / Q000494:pharmacology; D018170:Sumatriptan / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D012668:Trigeminal Ganglion / Q000502:physiology*
D011506:Proteins; D011759:Pyrrolidines; C085263:CP 122288; D005070:Evans Blue; D018170:Sumatriptan