Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.
A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI, shares 66% identity and 76% similarity with M. Cvi JI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.
Xu
M
M
Department of Biochemistry and Molecular Biology and the Center for Gene Regulation, 308 Althouse Laboratory,The Pennsylvania State University, University Park, PA 16802-4500, USA.
Kladde
M P
MP
Van Etten
J L
JL
Simpson
R T
RT
D000595:Amino Acid Sequence; D002708:Chlorella / Q000821:virology*; D002843:Chromatin / Q000235:genetics; D002874:Chromosome Mapping / Q000379:methods; D003001:Cloning, Molecular; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics* / Q000378:metabolism*; D017931:DNA Primers; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005814:Genes, Viral; D015698:Genomic Library; D008745:Methylation; D008969:Molecular Sequence Data; D017862:Phycodnaviridae / Q000201:enzymology / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
D002843:Chromatin; D017931:DNA Primers; D015226:Dinucleoside Phosphates; C054544:2'-deoxyguanylyl-(3'-5')-2'-deoxycytidine; D004248:DNA (Cytosine-5-)-Methyltransferases