New, sensitive high-performance liquid chromatography method for the determination of slaframine in plasma and milk.
Slaframine was derivatized precolumn with fluorescamine. The derivatized slaframine was chromatographed isocratically using HPLC on a Hamilton PRP-1 C18 polymeric column with fluorescence detection. By using fluorescent derivatization, sensitivity was increased 100-fold over previously reported GC methods. A liquid-liquid partition was used to extract slaframine from plasma with a 95% recovery and a CV% of 8.4. A solid-phase extraction was used to extract slaframine from milk with a 91% recovery and a CV% of 9.8.
Veterinary Diagnostic Laboratory, Iowa State University, Ames 50011, USA.
P M
PM
Imerman
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HM
Stahr
D000470:Alkaloids / Q000032:analysis* / Q000097:blood; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005450:Fluorescamine; D007202:Indicators and Reagents; D008892:Milk / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis* / Q000097:blood; D010949:Plasma / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D000470:Alkaloids; D007202:Indicators and Reagents; D009183:Mycotoxins; D005450:Fluorescamine; C009275:slaframine