[Role of protein kinase C in the regulation of Ca-responses induced by purinergic agonists and thapsigargin in rat peritoneal macrophages].
The effect of protein kinase C activating phorbol ester, phorbol-12-myristate-13-acetate (PMA), on purinergic agonists- and thapsigargin-induced Ca2+ signals in Fura-2 loaded rat peritoneal macrophages was investigated. PMA (100 ng/ml) was shown to inhibit 200 muM ATP- or 200 microM UTP-evoked Ca2+ entry in macrophages. Protein kinase C activation by PMA also inhibits the store-dependent or "capacitative" Ca2+ influx stimulated by emptying the intracellular Ca2+ stores with endoplasmic Ca(2+)-ATPase inhibitor thapsigargin (0.5 microM). Inhibition of entry by PMA was fully prevented by protein kinase C inhibitor 50 microM H-7. These data are compatible with the important role played by protein kinase C in the control of Ca2+ entry in rat peritoneal macrophages.
Physiological Research Institute, St. Petersburg University.
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Krutetskaia
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Lebedev
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Krutetskaia
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000032:analysis; D000252:Calcium-Transporting ATPases / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005456:Fluorescent Dyes; D016257:Fura-2; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D011493:Protein Kinase C / Q000502:physiology*; D058913:Purinergic Agonists; D051381:Rats; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D019284:Thapsigargin / Q000494:pharmacology*; D014544:Uridine Triphosphate / Q000494:pharmacology
D004791:Enzyme Inhibitors; D005456:Fluorescent Dyes; D058913:Purinergic Agonists; D019284:Thapsigargin; D000255:Adenosine Triphosphate; D011493:Protein Kinase C; D000252:Calcium-Transporting ATPases; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium; D016257:Fura-2; D014544:Uridine Triphosphate