Identification and expression of the Bacillus subtilis fructose-1, 6-bisphosphatase gene (fbp).
The Bacillus subtilis fbp gene encoding fructose-1,6-bisphosphatase (FBPase) was originally identified as yydE. The fbp gene was expressed at a fairly constant level in cells undergoing glycolysis or gluconeogenesis. fbp transcription was initiated 94 bp upstream of the translation initiation codon, resulting in a 2.4-kb monocistronic transcript. Interestingly, B. subtilis FBPase exhibited no significant similarity to other FBPases in protein sequence databases.
Department of Biotechnology, Faculty of Engineering, Fukuyama University, Fukuyama 729-0292, Japan. yfujita@fubt.fukuyama-u.ac.jp
Y
Y
Fujita
K
K
Yoshida
Y
Y
Miwa
N
N
Yanai
E
E
Nagakawa
Y
Y
Kasahara
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D006597:Fructose-Bisphosphatase / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial
D006597:Fructose-Bisphosphatase