Structural characterization of a DNA duplex modeled on a DNA:RNA hybrid of the polypurine tract recognized by a reverse transcriptase.
The structure of d(TTAAAAGAAAAGGG):d(CCCTTTTCTTTTAA) has been characterized by NMR. The minor grooves of the two dA-tracts are suggested to be rather narrow, and the portion linking the two dA tracts exhibits a slightly deviated structure from a standard B DNA, in order to maintain the narrowness of the minor groove. The structure of the dG-tract is also slightly deviated. Additionally, specific broadening of resonances is observed for the residues at or near the junction between the dA-tract and the dG-tract, suggesting local structural polymorphology.
Department of Bioengineering, Faculty of Engineering, Yokohama National University, Japan.
M
M
Kanagawa
Y
Y
Okada
S
S
Uesugi
H
H
Doi
M
M
Katahira
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry*; D011687:Purines / Q000737:chemistry* / Q000378:metabolism; D012313:RNA / Q000737:chemistry*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D009692:Nucleic Acid Heteroduplexes; D011687:Purines; D012313:RNA; D004247:DNA; D012194:RNA-Directed DNA Polymerase