Multiple-quantum filtered 17Q and 23Na NMR analysis of mitochondrial suspensions.
The fraction of strongly- and weakly-bound water molecules within mitochondrial suspensions, determined using three-quantum filtered 17O NMR relaxation analysis, was found to be large in comparison with that in erythrocytes and concentrated solutions of bovine serum albumen. It is suggested that bound water, together with regulation of mitochondrial matrix volume, may be an important controlling factor in the modulation of enzymic activity in the matrix. A spin I = 5/2 Jeener-Broekaert experiment and a four-quantum filtration experiment were used to demonstrate the absence of orientationally ordered water molecules within the mitochondrion. In contrast, the mitochondrial sodium environment was shown to be highly ordered using a spin I = 3/2 Jeener-Broekaert experiment.
Department of Biochemistry, University of Oxford, UK. scw@physchem.ox.ac.uk
S M
SM
Grieve
B
B
Wickstead
A M
AM
Torres
P
P
Styles
S
S
Wimperis
P W
PW
Kuchel
D000818:Animals; D002417:Cattle; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008930:Mitochondria, Liver / Q000737:chemistry* / Q000378:metabolism; D010100:Oxygen / Q000032:analysis* / Q000097:blood; D010103:Oxygen Isotopes; D011789:Quantum Theory; D051381:Rats; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism; D012964:Sodium / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D013535:Suspensions; D014867:Water / Q000378:metabolism
D010103:Oxygen Isotopes; D013535:Suspensions; D014867:Water; D012710:Serum Albumin, Bovine; D012964:Sodium; D010100:Oxygen