ERE environment- and cell type-specific transcriptional effects of estrogen in normal endometrial cells.
Our previous results have suggested a repression of E2 (17beta-estradiol) effect on the c-fos gene of cultured guinea-pig endometrial cells. To investigate this repression, the expression of three human c-fos gene recombinants, pFC1-BL (-2250/+41), pFC2-BL (-1400/+41) and pFC2E (-1300/-1050 and -230/+41), known to be E2-responsive in Hela cells, was studied in stromal (SC) and glandular epithelial cells (GEC). In both cellular types, pFC1-BL was not induced by E2, even in the presence of growth factors or co-transfected estrogen receptor. The pattern of pFC2-BL and pFC2E expression was strikingly different and depended on the cellular type: pFC2-BL and pFC2E induction was restricted to the glandular epithelial cells and did not occur in the SCs. We argue for a repression of E2 action which is dependent on the estrogen-responsive cis-acting element (ERE) environment and also cell type-specific involving DNA/protein and/or protein/protein interactions with cellular type-specific factors.
I.E.T.G., Bâtiment INSERM, Besançon, France.
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Lascombe
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Sallot
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Vuillermoz
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Weisz
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Adessi
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Jouvenot
D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D016762:Genes, fos / Q000235:genetics; D006168:Guinea Pigs; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D011993:Recombinant Fusion Proteins; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012097:Repressor Proteins / Q000502:physiology*; D017154:Stromal Cells; D014158:Transcription, Genetic / Q000235:genetics*; D014162:Transfection / Q000379:methods
D007328:Insulin; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D004958:Estradiol; D004815:Epidermal Growth Factor