Yeast coactivator MBF1 mediates GCN4-dependent transcriptional activation.
Transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. The coactivator multiprotein bridging factor 1 (MBF1) was originally identified as a bridging molecule that connects the Drosophila nuclear receptor FTZ-F1 and TATA-binding protein (TBP). The MBF1 sequence is highly conserved across species from Saccharomyces cerevisiae to human. Here we provide evidence acquired in vitro and in vivo that yeast MBF1 mediates GCN4-dependent transcriptional activation by bridging the DNA-binding region of GCN4 and TBP. These findings indicate that the coactivator MBF1 functions by recruiting TBP to promoters where DNA-binding regulators are bound.
Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, The Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan.
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Takemaru
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Harashima
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Ueda
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Hirose
D000595:Amino Acid Sequence; D000818:Animals; D002148:Calmodulin-Binding Proteins; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D005656:Fungal Proteins / Q000737:chemistry / Q000378:metabolism*; D051717:Fushi Tarazu Transcription Factors; D015966:Gene Expression Regulation, Fungal; D018398:Homeodomain Proteins; D006801:Humans; D019476:Insect Proteins; D008969:Molecular Sequence Data; D011494:Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D054339:Steroidogenic Factor 1; D035181:TATA-Box Binding Protein; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism; D015533:Transcriptional Activation
D002148:Calmodulin-Binding Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; C115911:EDF1 protein, human; C485470:Edf1 protein, mouse; D005656:Fungal Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D019476:Insect Proteins; C491465:MBF1 protein, Drosophila; C106951:MBF1 protein, S cerevisiae; D018160:Receptors, Cytoplasmic and Nuclear; D029701:Saccharomyces cerevisiae Proteins; D054339:Steroidogenic Factor 1; D035181:TATA-Box Binding Protein; D015534:Trans-Activators; D014157:Transcription Factors; C497169:ftz protein, Drosophila; C499712:nuclear hormone receptor FTZ-F1, Drosophila; D011494:Protein Kinases