Specificity of isoelectric focusing-purified antigens in the diagnosis of human cysticercosis.
Specific antigens were isolated from the cystic fluid of larval Taenia solium by preparative isoelectric focusing (PIEF). A total of 20 fractions were produced by a rotating ampholine column with pI 3-10 ampholytes. The specificity of each fraction (F) was tested by double-antibody enzyme-linked immunosorbent assay (ELISA) using antisera from patients suffering from cysticercosis or one of six other parasitic diseases. F8-F15 cross-reacted strongly with sera from patients with hydatidosis. F9 and F10 also cross-reacted with the antisera against ascariasis and F15, with antisera against angiostrongylosis. However, F16 and F17 were highly specific as they yielded no cross-reaction with any of the heterologous antisera. PIEF is a good method for the production of specific antigens from larval T. solium because it is easy to perform and relatively inexpensive to run.
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Department of Zoology, The University of Hong Kong, Hong Kong.
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D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology
D000947:Antigens, Helminth