The role of a new liquid-solid scintillator, a mixture of DPO, POPOP and Epon 812, for enhancement of autoradiograms with tritium agents.
It is desirable to shorten expose time in 3H-autoradiography. We developed a new solid scintillator, a mixture of DPO, POPOP and Epon 812, for whole-body autoradiography. The new scintillator is originally in a sol form, but quickly transforms to a solid homogeneous film following exposure to room air. The new scintillator enabled whole-body autoradiography to be developed faster when compared with a liquid scintillator or commercially available reagents. We also demonstrated that the new scintillator gave consistently high counting performance and thus shortened duration of exposure when used in whole-body autoradiography, whereas commercially available enhancers were found to be unsuitable for autoradiography, resulting in dappled images. Furthermore the quantitative analyses of the autoradiograms by a densitometer demonstrated the highest density, and ratio with this new solid scintillator among those with a liquid scintillator and commercially available reagents. In conclusion, a new solid scintillator, a mixture of DPO, POPOP and Epon 812, is a useful and cost effective enhancer for the whole-body autoradiography of tritum compounds.
Department of Radioisotope Center, Kitasato University School of Medicine, Kanagawa, Japan.
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D000818:Animals; D000970:Antineoplastic Agents / Q000493:pharmacokinetics; D001345:Autoradiography / Q000379:methods*; D004853:Epoxy Resins; D006801:Humans; D007202:Indicators and Reagents; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010080:Oxazoles; D011471:Prostatic Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D011877:Radionuclide Imaging; D012588:Scintillation Counting / Q000379:methods*; D013498:Suramin / Q000493:pharmacokinetics; D014183:Transplantation, Heterologous; D014316:Tritium
D000970:Antineoplastic Agents; D004853:Epoxy Resins; D007202:Indicators and Reagents; D010080:Oxazoles; D014316:Tritium; C028380:POPOP; D013498:Suramin; C004875:EPON; C009012:2,5-diphenyloxazole