Overproduction of acetyl-coenzyme A synthetase isoenzymes in respiring Saccharomyces cerevisiae cells does not reduce acetate production after exposure to glucose excess.
To investigate whether the production of acetate which occurs after exposure of respiring Saccharomyces cerevisiae cells to excess glucose can be reduced by overproduction of acetyl-CoA synthetase (ACS, EC 6.2.1.1), the ACS1 and ACS2 genes were introduced on multi-copy plasmids. For each isoenzyme, the level in glucose-limited chemostat cultures was increased by 3-6-fold, relative to an isogenic reference strain. However, ACS overproduction did not result in a reduced production of acetate after a glucose pulse (100 mmol l-1) to these cultures. This indicates that a limited capacity of ACS is not the sole cause of acetate accumulation in S. cerevisiae.
Department of Microbiology and Enzymology, Kluyver Institute of Biotechnology, Delft University of Technology, The Netherlands.
P
P
de Jong-Gubbels
M A
MA
van den Berg
M A
MA
Luttik
H Y
HY
Steensma
J P
JP
van Dijken
J T
JT
Pronk
D000106:Acetate-CoA Ligase / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism*; D003001:Cloning, Molecular; D000431:Ethanol / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D005800:Genes, Fungal; D005947:Glucose / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics
D000085:Acetates; D005656:Fungal Proteins; D007527:Isoenzymes; D000431:Ethanol; D000106:Acetate-CoA Ligase; D005947:Glucose