In vitro antiplasmodial activity of stem and root extracts of Nauclea latifolia S.M. (Rubiaceae).
Aqueous extracts from Nauclea latifolia S.M. (Rubiaceae), a plant commonly used in Ivory Coast by traditional healers for the treatment of malaria, were tested on two strains of Plasmodium faliparum: FcB1-Colombia (chloroquine-resistant) and a Nigerian strain (chloroquine-sensitive). The extracts were obtained from stems and roots of the plant in two forms, infusion and decoction, both methods used by most traditional healers. The in vitro activity of N. latifolia extracts on P. falciparum was assessed both visually and by a radioactive method. The visual analysis allowed determination of the time of extract action on the erythrocytic cycle, as well as the parasitic stage of most inhibitory effect. Similar results were obtained applying fresh, frozen or lyophilized extracts. The IC50 values determined were within the range already reported for other antimalarial plants such as Azadirachta indica A. Juss (Meliaceae) or Artemisia annua L. (Asteraceae). Aqueous extracts of N. latifolia inhibited P. falciparum (FcB1 strain) mainly at the end of the erythrocytic cycle (32nd to 48th hour).
Laboratoire d'Immunologie et Parasitologie, Faculté de Pharmacie, Montpellier, France.
F
F
Benoit-Vical
A
A
Valentin
V
V
Cournac
Y
Y
Pélissier
M
M
Mallié
J M
JM
Bastide
D000818:Animals; D000962:Antimalarials / Q000302:isolation & purification* / Q000494:pharmacology; D007560:Cote d'Ivoire; D016488:Medicine, African Traditional; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D010963:Plasmodium falciparum / Q000187:drug effects
D000962:Antimalarials; D010936:Plant Extracts