Autofluorescence of living cells.
We have investigated the autofluorescence of viable mammalian cells (DU-145 and V79) with a confocal laser scanning microscope equipped with a UV laser. Our aim was to investigate the autofluorescence dependence on different treatments in mitochondria and lysosomes by using different reagents and to improve the confocal laser scanning microscope image quality by deconvolution. The following conclusions were drawn from the results: (1) not all of the autofluorescence comes from mitochondria; (2) one can significantly affect the signal which comes from the mitochondria; (3) the other organelles involved are probably lysosomes; (4) it is harder to affect the autofluorescence signal from the lysosomes than that from the mitochondria, and (5) deconvoluted autofluorescence images provide better information than undeconvoluted ones.
Institute for Medical Radiobiology, University of Zurich, Switzerland.
H
H
Andersson
T
T
Baechi
M
M
Hoechl
C
C
Richter
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005347:Fibroblasts; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism; D005453:Fluorescence; D005456:Fluorescent Dyes; D008062:Lipofuscin / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism; D008322:Mammals; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010545:Peroxides / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D014118:Toxins, Biological / Q000494:pharmacology; D020122:tert-Butylhydroperoxide
D005456:Fluorescent Dyes; D008062:Lipofuscin; D010545:Peroxides; D014118:Toxins, Biological; D012402:Rotenone; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D020122:tert-Butylhydroperoxide