Characterisation of the roles of CD95 and CD95 ligand in cord blood.
The increasing use of umbilical cord blood in transplantation has led to a renewed interest in the immunological characterisation of this material. This study addresses the question of whether the CD95 molecule and its ligand are expressed and are functional in mediating cell death in cord blood mononuclear cells. These molecules have a crucial role in the homeostasis of haematopoietic cell populations in the adult and also contribute to graft-versus-host disease. CD95 is the most well studied receptor mediating a signal for cell death by apoptosis and its inducible ligand has been demonstrated to mediate cell death of multiple types of CD95 expressing cells. The object of this study was to examine whether cord blood mononuclear cells could behave either as targets for CD95-mediated cell death or as mediators of cell death due to the expression of CD95L. The results of this study lead us to suggest that cord blood mononuclear cells enjoy some immunological privilege due to the relatively low level of expression of CD95 (in comparison with adult peripheral blood lymphocytes) and the expression of the CD95 ligand.
INSERM U396, Institut des Cordeliers, Paris, France.
B
B
Drénou
C
C
Choqueux
A
A
El Ghalbzouri
V
V
Blancheteau
A
A
Toubert
D
D
Charron
N
N
Mooney
D000328:Adult; D017209:Apoptosis; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein; D005312:Fetal Blood / Q000502:physiology*; D006801:Humans; D007231:Infant, Newborn; D008024:Ligands; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000502:physiology*; D009000:Monocytes / Q000502:physiology; D019014:fas Receptor / Q000096:biosynthesis / Q000502:physiology*
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008024:Ligands; D008562:Membrane Glycoproteins; D019014:fas Receptor