Clinical analysis of human urinary proteins using high resolution electrophoretic methods.
The application of isoelectric focusing (IEF), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional electrophoresis (2-DE) and capillary electrophoresis (CE) for high resolution electrophoretic analysis of human urinary proteins is reviewed. In each case, the information is tabulated chronologically with details of sample preparation, electrophoretic system, detection method and clinical application. The text includes an historical perspective of the use of each method for urinalysis and a detailed review of the application of the methods to the investigation of renal disease, renal transplantation, Bence Jones proteinuria (BJP), diabetes mellitus, cadmium toxicity, nephrolithiasis and cancers of the urogenital tract.
Analytical Biochemistry Group, School of Health Sciences, The University of Sunderland, Great Britain. tom.marshall@sunderland.ac.uk
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T
Marshall
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Williams
D004586:Electrophoresis; D019075:Electrophoresis, Capillary; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D011507:Proteinuria; D012967:Sodium Dodecyl Sulfate
D012967:Sodium Dodecyl Sulfate