Detection of bovine viral diarrhea virus (BVDV) using reverse transcription polymerase chain reaction assay.
The reverse transcription polymerase chain reaction (RT-PCR) was applied to detect bovine viral diarrhea virus (BVDV) for the rapid diagnosis. The primers were selected from the p80 region of BVDV gene. The RT-PCR assay detected all of the 17 BVDV strains tested including cytopathogenic and non-cytopathogenic strains, while specific amplification was not observed from 17 bovine viruses other than BVDV. Detection limit of the assay was 10(1.5) TCID50/ml. Sera and organ samples were collected from four field bovine viral diarrhea-mucosal disease (BVD-MD) cases; mucosal disease, abortion, diarrhea and persistent infection. The RT-PCR assay detected BVDV from those samples more than conventional virus isolation method.
Uruno
K
K
Zen-noh Institute of Animal Health, Chiba, Japan.
Shibata
I
I
Nakane
T
T
D000034:Abortion, Veterinary / Q000821:virology; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000175:diagnosis*; D002417:Cattle; D003967:Diarrhea / Q000209:etiology / Q000821:virology; D005260:Female; D016679:Genome, Viral; D008297:Male; D018482:Muscle, Skeletal / Q000821:virology; D010576:Pestivirus / Q000235:genetics / Q000302:isolation & purification*; D011247:Pregnancy; D012367:RNA, Viral / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012367:RNA, Viral