Design and evaluation of an in-house HIV-1 (group M and O), SIVmnd and SIVcpz antigen capture assay.
An enzyme-linked immuno-sorbent assay (ELISA) for the detection of human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIVcpz/SIVmnd) antigens was designed using immunoreagents from naturally infected individuals, and compared to the commercially available Vironostika HIV-1 Antigen Microelisa System (Organon Teknika). The in-house assay proved to be specific for HIV-1 isolates belonging to group M (A-H) and group O and for SIVcpz and SIVmnd isolates, but was less sensitive than the Vironostika HIV-1 Antigen Microelisa System, except for SIVmnd. For the strains belonging to HIV-2, SIVmac and SIVagm, the in-house assay could not detect antigen to an appreciable degree. This study shows that a considerably less expensive but sufficiently accurate HIV-1 antigen capture assay can be developed to monitor HIV-1 (group M and O), SIVcpv and SIVmnd antigen in the supernatants of virus cultures.
Institute of Tropical Medicine, Department of Microbiology, Antwerp, Belgium.
E
E
Beirnaert
B
B
Willems
M
M
Peeters
A
A
Bouckaert
L
L
Heyndrickx
P
P
Zhong
K
K
Vereecken
S
S
Coppens
D
D
Davis
P
P
Ndumbe
W
W
Janssens
G
G
van der Groen
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000032:analysis*; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D015488:HIV Antigens / Q000097:blood*; D016655:HIV Core Protein p24 / Q000097:blood / Q000276:immunology; D015497:HIV-1 / Q000145:classification / Q000276:immunology*; D015498:HIV-2 / Q000145:classification / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000302:isolation & purification; D012680:Sensitivity and Specificity; D015302:Simian Immunodeficiency Virus / Q000145:classification / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D015488:HIV Antigens; D016655:HIV Core Protein p24; D007106:Immune Sera; D007074:Immunoglobulin G