Coagulin, a bacteriocin-like inhibitory substance produced by Bacillus coagulans I4.
A protease-sensitive antibacterial substance produced by Bacillus coagulans I4 strain, isolated from cattle faeces, was classified as a bacteriocin-like inhibitory substance and named coagulin. The inhibitory spectrum included B. coagulans and unrelated bacteria such as Enterococcus, Leuconostoc, Oenococcus, Listeria and Pediococcus. Coagulin was stable at 60 degrees C for 90 min, at a pH ranging from 4 to 8 and appeared to be unaffected by alpha-amylase, lipase or organic solvents (10% v/v). Coagulin exhibited a bactericidal and a bacteriolytic mode of action against indicator cells. The apparent molecular mass was estimated to be about 3-4 kDa by SDS-PAGE. The B. coagulans I4 strain harbours a plasmid, pI4, approximately 14 kb in size. Novobiocin curing experiments yielded two derivatives that no longer produced the bacteriocin-like inhibitory substance. Plasmid content of these two derivatives showed that one had lost pI4, whereas the second harboured a deleted form of this plasmid, thus suggesting a plasmid location for the genes for coagulin production.
Laboratoire de Microbiologie et Biochimie Appliquées, ENITA Bordeaux, Gradignan, France.
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Le Marrec
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D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001407:Bacillus / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000494:pharmacology; D001430:Bacteriocins / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000494:pharmacology; D001483:Base Sequence; D002417:Cattle; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000382:microbiology; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009675:Novobiocin / Q000494:pharmacology; D019105:Random Amplified Polymorphic DNA Technique
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001430:Bacteriocins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C114379:coagulin, Bacillus; D009675:Novobiocin