Highly efficient oligonucleotide transfer into intact yeast cells using square-wave pulse electroporation.
Here, we present a rapid and reproducible procedure based on square-wave pulse electroporation that allows efficient penetration of synthetic oligonucleotides into intact yeast cells. This procedure was successfully used to modify the yeast genome with small amounts of oligonucleotide.
Cancer, CNRS UPR9079 Institut Gustave Roussy, Villejuif, France.
F X
FX
Barre
L M
LM
Mir
Y
Y
Lécluse
A
A
Harel-Bellan
D000595:Amino Acid Sequence; D001483:Base Sequence; D018274:Electroporation / Q000295:instrumentation / Q000379:methods*; D005656:Fungal Proteins / Q000235:genetics; D018014:Gene Transfer Techniques; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000378:metabolism*; D015003:Yeasts / Q000166:cytology / Q000235:genetics*
D005656:Fungal Proteins; D009841:Oligonucleotides