Beta 2-microglobulin-dependent T cells are not necessary for alloantigen-induced Th2 responses after neonatal induction of lymphoid chimerism in mice.
We have analyzed the requirement for beta 2-microglobulin (beta 2m)-dependent T cells in the generation of allogeneic Th2 responses in vivo. A neonatal injection of semiallogeneic cells in BALB/c mice induces a state of chimerism that promotes the differentiation of donor-specific CD4+ T cells toward the Th2 phenotype. Polyclonal T-B cell interactions occur in this model between host Th2 and donor B cells, resulting in the production of IgE Abs. IgE production and Th2-priming are critically dependent upon the early production of IL-4. Our data in the present paper demonstrate that: 1) IgE synthesis and the up-regulation of MHC class II and CD23 molecules on B cells are independent of beta 2m expression in the host, 2) no difference in the induction of CD4 alloreactive Th2 cells could be observed between beta 2m-/- and their wild-type control littermates when Th2-priming was measured in adult mice, and 3) the Th2 response and IgE production is induced in the complete absence of beta 2m-dependent T cells both in the host and in the inoculum. Therefore, using a variety of assays, we could not demonstrate diminished responses in mice with a disrupted beta 2m gene in this model of Th2-mediated allogeneic interaction, indicating that beta 2m-dependent NK1.1+ and CD8+ T cells are not required for the generation of alloreactive Th2 responses in vivo.
Foucras
G
G
Institut National de la Santé et de la Recherche Médicale U.28, Université Paul Sabatier, Hôpital Purpan, Toulouse, France.
Coureau
C
C
Beijleveld
L
L
Druet
P
P
Saoudi
A
A
Guéry
J C
JC
D000818:Animals; D000831:Animals, Newborn / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D007114:Immunization; D007073:Immunoglobulin E / Q000096:biosynthesis; D007279:Injections, Subcutaneous; D015847:Interleukin-4 / Q000096:biosynthesis; D007519:Isoantigens / Q000008:administration & dosage / Q000276:immunology / Q000502:physiology*; D008213:Lymphocyte Activation / Q000235:genetics*; D008212:Lymphocyte Depletion; D017710:Lymphocyte Transfusion; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011828:Radiation Chimera / Q000235:genetics / Q000276:immunology*; D013154:Spleen / Q000637:transplantation; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology* / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000172:deficiency / Q000235:genetics / Q000502:physiology*
D007519:Isoantigens; D001613:beta 2-Microglobulin; D015847:Interleukin-4; D007073:Immunoglobulin E