Effect of seasonal acclimatization on the expression of the carp transcription factor Pit-1.
We isolated a clone comprising four exons of the carp Pit-1 gene. Using synthetic oligonucleotide probes derived from the carp Pit-1 sequence Pit-1 expression was assessed by in situ hybridization in pituitary sections from summer- and winter-acclimatized carp. Semiquantitative analyses of the hybridization signals revealed a significant higher Pit-1 expression in the proximal pars distalis (PPD) and pars intermedia (PI) of the pituitary glands from summer-acclimatized carp, compared to the winter-acclimatized fish. In both adaptive states, relative to the PPD and PI, only a basal Pit-1 expression was detected in the rostral pars distalis. Thus, during seasonal acclimatization of an eurythermal fish, Pit-1 seems to be involved in the mechanisms that underlie the compensatory response.
Institute of Biochemistry, Faculty of Sciences, Universidad Austral de Chile, Valdivia, Chile.
G
G
Kausel
M I
MI
Vera
J
J
Figueroa
J
J
Hernández
R
R
San Martin
A
A
Molina
V M
VM
Chavez
M
M
Muller
J
J
Martial
M
M
Krauskopf
D000064:Acclimatization; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D015698:Genomic Library; D017403:In Situ Hybridization; D008297:Male; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D012621:Seasons; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000235:genetics*
D004268:DNA-Binding Proteins; D015345:Oligonucleotide Probes; D050817:Transcription Factor Pit-1; D014157:Transcription Factors