Improved restriction landmark cDNA scanning and its application to global analysis of genes regulated by nerve growth factor in PC12 cells.
Restriction landmark cDNA scanning (RLCS) is a novel method by which more than 1000 genes can be simultaneously and quantitatively displayed as two-dimensional gel spots. Here we present an adaptation that allows an individual spot to correspond to a unique gene species without redundancy in more than two gel patterns. Using this improved RLCS, we examined global changes on the gene expression of PC12 cells before and after treatment with nerve growth factor. Among a total of 3000 spots, 21 (0.70%) and 91 (3.03%) spots newly appeared and became more intense with treatment. On the other hand, 15 (0.50%) and 44 (1.47%) spots disappeared, becoming less intense with treatment. These observations suggest that approx. 6% of the detected PC12 genes are up-(3.73%) or down-(1.97%) regulated when the cells differentiate to neuronal cells. In comparison with the results obtained using the expressed-sequence-tag approach, previously reported by Lee et al. (Proc. Natl. Acad. Sci. USA 92 (1995) 8303-8307), RLCS should be useful for quantitatively examining the global change of differentially expressed genes of various expression levels.
Shionogi Institute for Medical Science, Osaka, Japan.
K
K
Mayumi
T
T
Yaoi
J
J
Kawai
S
S
Kojima
S
S
Watanabe
H
H
Suzuki
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D018076:DNA, Complementary / Q000032:analysis*; D015870:Gene Expression; D009414:Nerve Growth Factors / Q000494:pharmacology* / Q000502:physiology; D016716:PC12 Cells; D051381:Rats; D015183:Restriction Mapping / Q000379:methods*; D016324:Sequence Tagged Sites
D018076:DNA, Complementary; D009414:Nerve Growth Factors