Involvement of IL-10 in induction of autoimmune hemolytic anemia in anti-erythrocyte Ig transgenic mice.
Activation of peritoneal B-1 cells triggers autoimmune anemia in anti-erythrocyte Ig transgenic mice (HL mice). Numbers of peritoneal B-1 cells and Ig-producing cells were negligible in the T cell-deficient HL mice generated by the cross with RAG-2-/- mice (RAG-2-/- x HL mice). Proliferation and activation of B-1 cells in RAG-2-/- x HL mice were recovered by fetal thymus transfer, indicating involvement of T cells in B-1 cell-mediated autoimmune hemolytic anemia. Involvement of T cells in proliferation and activation of B-1 cells could be by-passed by administration of lipopolysaccharide (LPS), IL-5 or IL-10 to RAG-2-/- x HL mice. Administration of LPS elevated the serum IL-10 level in HL, RAG-2-/- x HL and normal mice. Proliferation and activation of B-1 cells were blocked by an anti-IL-10 antibody in conventionally bred as well as LPS-treated HL mice. Taken together, IL-10 plays a pivotal role in activation of peritoneal B-1 cells.
Department of Medical Chemistry, Faculty of Medicine, Kyoto University, Japan.
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Nisitani
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Sakiyama
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Honjo
D000744:Anemia, Hemolytic, Autoimmune / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001323:Autoantibodies / Q000097:blood; D001402:B-Lymphocytes / Q000276:immunology*; D018956:CD5 Antigens / Q000276:immunology; D002452:Cell Count; D017637:Clonal Deletion / Q000276:immunology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D006400:Hematocrit; D016753:Interleukin-10 / Q000097:blood / Q000276:immunology* / Q000494:pharmacology; D015848:Interleukin-5 / Q000097:blood / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010537:Peritoneum / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000196:embryology / Q000276:immunology
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D018956:CD5 Antigens; D004268:DNA-Binding Proteins; D015848:Interleukin-5; D008070:Lipopolysaccharides; C499308:Rag2 protein, mouse; C064655:V(D)J recombination activating protein 2; D016753:Interleukin-10