Mytilus edulis hemolymph contain prodynorphin.
We have characterized a prodynorphin (prodyn) molecule in hemocytes and hemolymph of the bivalve mollusk Mytilus edulis. The ca. 16-kDa protein was purified by cut-off filtration prepurification, anti-leucine-enkephalin affinity column separation followed by reversed-phase HPLC. Its primary sequence was determined by Edman degradation, endoproteinase Glu-C digestion and CNBr treatment. Mytilus prodyn contains, alpha-neo-endorphin, dynorphin-A and dynorphin-B at the C-terminus, exhibiting 100, 70.5 and 85% of identity with the rat prodyn-derived counterparts, respectively. The number of leucine-enkephalins in this precursor is identical to that found in vertebrates. Mytilus prodyn is distinguished from that found in leeches in that the N-terminus is longer. Additionally, by sequence comparison, the presence of an orphanin FQ-like peptide, exhibiting 50% sequence similarity with that found in mammals, is demonstrated. This report constitutes the first complete biochemical characterization of a prodyn in a non-parasitic invertebrate and mollusk.
Stefano
G B
GB
Multidisciplinary Center for the Study of Aging, State University of New York, College at Old Westbury, 11568-0210, USA.
Salzet-Raveillon
B
B
Salzet
M
M
D000595:Amino Acid Sequence; D000818:Animals; D049872:Bivalvia / Q000737:chemistry*; D004745:Enkephalins / Q000097:blood*; D006458:Hemolymph / Q000737:chemistry*; D006801:Humans; D007865:Leeches; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000097:blood*; D051381:Rats; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013552:Swine
D004745:Enkephalins; D011498:Protein Precursors; C033660:preproenkephalin