5% Me2SO is sufficient to preserve stem cells derived from cord blood.
On the basis of historical findings, 10% dimethylsulfoxide is still used to cryopreserve stem cells. We studied a final concentration of 5% dimethylsulfoxide in cryopreservation of cord blood-derived stem cells in autologous plasma. In our opinion, a final concentration of 5% dimethylsulfoxide in autologous plasma without further additives is sufficient for cryopreservation of cord blood stem cells in a banking routine.
German Red Cross, Blood Center Mannheim and Medical Faculty Mannheim, Germany.
E
E
Richter
H
H
Eichler
D
D
Raske
A
A
Leveringhaus
W
W
Zieger
M
M
Kerowgan
S F
SF
Goldmann
D001771:Blood Banks; D001793:Blood Preservation / Q000379:methods*; D001800:Blood Specimen Collection / Q000379:methods; D015925:Cryopreservation / Q000379:methods; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005312:Fetal Blood; D006412:Hematopoietic Stem Cells; D006801:Humans
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide