Characterization of the BcLF1 promoter in Epstein-Barr virus.
The location of the BcLF1 promoter of Epstein-Barr virus (EBV) has been identified by primer extension, which indicates that the +1 site of the BcLF1 mRNA is located at nucleotide 137676 of the EBV genome. According to deletion analysis, the region upstream from nucleotide -38 is not essential for transcription of BcLF1. A 23 bp region in the promoter, from nucleotide -38 to -16, was identified as necessary for regulating the expression of BcLF1, i.e. the promoter activity is activated by 12-O-tetradecanoylphorbol 13-acetate but is repressed by phosphonoacetic acid. The results presented also demonstrate that the oriLyt sequence in cis is essential for enhancing the expression of BcLF1.
Chang
P J
PJ
Institute of Microbiology and Immunology, National Yang-Ming University, Shih-Pai, Taipei, Taiwan.
Chang
Y S
YS
Liu
S T
ST
D002213:Capsid / Q000235:genetics*; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D011401:Promoter Regions, Genetic