Effects of aphidicolin on Entamoeba histolytica growth and DNA synthesis.
We have previously demonstrated that DNA polymerase activity of Entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative DNA polymerases. The present study was aimed to evaluate the effect of aphidicolin on growth and DNA synthesis by this parasite. Aphidicolin blocked the growth of axenic E. histolytica strain HM-1:IMSS. DNA synthesis was also inhibited by aphidicolin when assayed by incorporation of [3H]thymidine into the DNA. The inhibitory effect of aphidicolin on the growth of E. histolytica was abrogated by removal of the drug, and exposure to 3 microg/ml of the drug for at least 48 hr had little effect on the viability. Synchronous growth was observed in the recovery phase after removal of aphidicolin.
Department of Tropical Medicine, Jikei University School of Medicine, Tokyo, Japan.
A
A
Makioka
H
H
Ohtomo
S
S
Kobayashi
T
T
Takeuchi
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000096:biosynthesis / Q000187:drug effects*; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D019384:Nucleic Acid Synthesis Inhibitors
D016054:DNA, Protozoan; D004791:Enzyme Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D016590:Aphidicolin