[Studies on DNA content in sperm of men with oligozoospermia].
The aim of our study was to analysis the ploidy of spermatozoa from oligozoospermic individuals. The study was performed in 48 patients with oligozoospermia. Spermatozoal DNA was analysed using MagiCal imaging cytometry. The percentage of aneuploid cells was determined using CYT-2 program. The results indicated aneuploidy in 73% of the examined patients. The amount of cells with abnormal DNA content ranged from 30 to 100%. Monitoring of the DNA in human spermatozoa may have practical applications in establishing the reasons for infertility as well as for screening of the semen for assisted reproduction techniques.
Zakładu Radiobiologii i Biologii Komórki Akademii Medycznej im. Karola Marcinkowskiego w Poznaniu.
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D
Skonieczna
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M
Kotwicka
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K
Filipiak
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W
Warchoł
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Z
Fischer
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JB
Warchoł
D000782:Aneuploidy; D004247:DNA / Q000235:genetics; D006238:Haploidy; D006801:Humans; D019044:Image Cytometry / Q000379:methods; D007248:Infertility, Male / Q000209:etiology; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000150:complications / Q000235:genetics*; D012189:Retrospective Studies; D013076:Sperm Count; D013094:Spermatozoa / Q000502:physiology
D004247:DNA