Ambineela, an unusual blue protein isolated from the archaeon Acidianus ambivalens.
A novel blue protein, named ambineela, was isolated from the soluble extract of the thermoacidophilic archaeon Acidianus ambivalens. In solution, the purified protein is a monomer with 50 kDa and has a basic character (pI approximately 8.7). The electronic spectrum shows two bands, centred at 395 and 625 nm (A625/A395 = 0.7). The protein does not contain any transition metal; its blue colour is due to an unidentified non-fluorescent cofactor, covalently bound to it. Ambineela N-terminal sequence exhibits a consensus ADP-binding region, suggesting that its unknown cofactor may comprise this molecule or an analogue.
Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal.
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D000244:Adenosine Diphosphate / Q000378:metabolism; D000595:Amino Acid Sequence; D019843:Archaeal Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017421:Sequence Analysis; D016943:Sulfolobaceae / Q000235:genetics / Q000378:metabolism*
D019843:Archaeal Proteins; C114092:ambineela protein, Acidianus ambivalens; D000244:Adenosine Diphosphate