Luteotropic hormone receptors in the ovary of the mink (Mustela vison) during delayed implantation and early-postimplantation gestation.
The reproductive cycle of the mink displays rigid seasonality and obligate embryonic diapause. After ovulation, the corpus luteum (CL) involutes, and it secretes basal progesterone until activated prior to implantation. To study changes in the relative abundance of luteal prolactin and LH receptor mRNA through gestation, ovaries and serum were collected from pregnant female mink at 2-day intervals (n = 3 per date) through embryonic diapause and CL activation (March 19-31) and at 5-day intervals through implantation and early-postimplantation gestation (March 31-April 15). To determine the effect of endogenous prolactin, mink received Alzet osmotic minipumps releasing 2 mg/day 2-bromo-alpha-ergocryptine (bromocriptine) or saline on March 19. Ovaries and serum were taken from 3 animals every 2 days until March 31. Prolactin receptor mRNA in ovaries was low during CL activation but increased 3-fold through embryo implantation. Its abundance correlated with prolactin binding to ovarian membranes and with circulating prolactin. Bromocriptine suppressed endogenous prolactin levels and prevented the increase in prolactin receptor mRNA. There was a transient peak in LH receptor mRNA in the ovaries at March 19-23, which declined to basal levels by March 25 and remained constant through midgestation. Bromocriptine prevented the preimplantation peak in LH receptor mRNA and reduced its abundance below pretreatment levels. The results suggest that prolactin up-regulates its receptor and maintains the LH receptor in the mink CL. The pattern of LH receptor mRNA argues for a role for LH in CL reactivation and termination of embryonic diapause in mink.
Douglas
D A
DA
Departments of Animal Science, McGill University, Montreal, Quebec, Canada H3A 1A1.
Houde
A
A
Song
J H
JH
Farookhi
R
R
Concannon
P W
PW
Murphy
B D
BD
D000818:Animals; D001971:Bromocriptine / Q000494:pharmacology; D003338:Corpus Luteum / Q000502:physiology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D010065:Embryo Implantation, Delayed; D047108:Embryonic Development; D005260:Female; D008907:Mink; D010053:Ovary / Q000737:chemistry / Q000378:metabolism*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D011388:Prolactin / Q000097:blood / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D011974:Receptors, LH / Q000235:genetics*; D011981:Receptors, Prolactin / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D011974:Receptors, LH; D011981:Receptors, Prolactin; D001971:Bromocriptine; D011374:Progesterone; D011388:Prolactin