Detection of Helicobacter pylori associated antigen and heat shock protein 60 on follicular dendritic cells in the germinal centres of low grade B cell lymphoma of gastric mucosa associated lymphoid tissue (MALT).
OBJECTIVE
To investigate the localisation of Helicobacter pylori antigens and the expression of human heat shock proteins (HSP) in stomachs affected by MALT lymphoma.


METHODS
Surgically resected stomachs from 24 patients with MALT lymphoma were immunostained with anti-H pylori rabbit antibodies (ORP-1 and ORP-2) and anti-human HSP60 mouse monoclonal antibodies (mAb) (LK-1 and LK-2).


RESULTS
Follicular dendritic cells of germinal centres in the stomachs affected by MALT lymphoma were immunostained with anti-H pylori polyclonal antibodies and with anti-human HSP60 mAb, as were the epithelial cells. None of the lymph node samples reacted.


CONCLUSIONS
Human HSP60, which cross reacts with anti-H pylori polyclonal antibodies, is often expressed on follicular dendritic cells in gastric MALT lymphoma tissues and may be aetiologically relevant to lymphomagenesis of MALT lymphoma.
Department of Pathology, Okayama University Medical School, Japan.
K
K
Kobayashi
K
K
Yokota
T
T
Yoshino
Y
Y
Kawahara
A
A
Dey
Y
Y
Hirai
K
K
Oguma
T
T
Akagi
D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis; D015153:Blotting, Western; D018834:Chaperonin 60 / Q000032:analysis*; D003713:Dendritic Cells / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D018442:Lymphoma, B-Cell, Marginal Zone / Q000276:immunology / Q000382:microbiology*; D013274:Stomach Neoplasms / Q000276:immunology / Q000382:microbiology*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60