Disruption studies of a Candida albicans gene, ELF1: a member of the ATP-binding cassette family.
A 3.6 kb gene (ELF1) with homology to the ATP-binding cassette (ABC) gene family has been isolated from genomic libraries of Candida albicans. Members of this gene family include both membrane transport proteins which confer a drug-resistance phenotype, and proteins whose functions are associated with protein translation. ELF1 (Elongation Like Factor) showed greatest homology with a Saccharomyces cerevisiae ORF (YPL226W), whose function is unknown, and lower homology with fungal elongation factor 3 (EF-3) genes. In comparison, homology with a gene conferring a drug-resistant phenotype (CDR1) was low. To understand the function of ELF1 in C. albicans, gene-knockout experiments were conducted using the hisG-URA3-hisG disruption cassette. Both single-copy (heterozygote) and double-disrupted strains in ELF1 were isolated. Phenotypically, the disrupted strains grew more slowly than wild-type and produced a mixture of large, irregular cells and apparently normal cells.
Department of Microbiology & Immunology, Georgetown University School of MedicineWashington DC 20007USA.
Joy
J
Sturtevant
Department of Microbiology & Immunology, Georgetown University School of MedicineWashington DC 20007USA.
Ronald
R
Cihlar
Department of Microbiology & Immunology, Georgetown University School of MedicineWashington DC 20007USA.
Richard
R
Calderone
D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000595:Amino Acid Sequence; D002176:Candida albicans / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics* / Q000302:isolation & purification; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed / Q000235:genetics; D010445:Peptide Elongation Factors / Q000235:genetics; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D014170:Transformation, Genetic
D018528:ATP-Binding Cassette Transporters; C115290:Elf1 protein, S pombe; D005656:Fungal Proteins; D010445:Peptide Elongation Factors; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; C111584:YEF3 protein, S cerevisiae; D000255:Adenosine Triphosphate