Increase of lipid hydroperoxides in the rat liver and kidney after administering ferric nitrilotriacetate.
Two hours after an intraperitoneal injection of ferric nitrilotriacetate to rats at a dose of 15 mg of Fe/kg of body weight, the level of lipid hydroperoxides, which was determined by a specific method involving chemical conversion into 1-naphthyldiphenylphosphine oxide and HPLC, had increased significantly in the liver (from 190.1 +/- 58.8 of the control to 467.1 +/- 175.8 pmol/mg of protein) and kidney (from 181.8 +/- 52.3 of the control to 405.9 +/- 22.7 pmol/mg of protein). These results demonstrate that oxidative stress was transiently increased by an iron overload in these tissues.
Department of Food Science and Nutrition, Nara Women's University, Japan.
K
K
Ikeda
F
F
Sun
K
K
Tanaka
S
S
Tokumaru
S
S
Kojo
D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid; D005290:Ferric Compounds / Q000008:administration & dosage / Q000633:toxicity*; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008054:Lipid Peroxides / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009571:Nitrilotriacetic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000633:toxicity; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
D002273:Carcinogens; D005290:Ferric Compounds; D008054:Lipid Peroxides; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate