Biopersistence of synthetic vitreous fibers and amosite asbestos in the rat lung following inhalation.
Fiber biopersistence as a major mechanism of fiber-induced pathogenicity was investigated. The lung biopersistence of 5 synthetic vitreous fibers (SVFs) and amosite asbestos was evaluated using the rat inhalation model. In contrast to several previous studies, this study examined fibers that dissolve relatively slowly in vitro at pH 7.4. Fisher rats were exposed for 5 days by nose-only inhalation to refractory ceramic fiber (RCF1a), rock (stone) wool (MMVF21), 2 relatively durable special application fiber glasses (MMVF32 or MMVF33), HT stonewool (MMVF34), amosite asbestos, or filtered air. Lung burdens were analyzed during 1 year post-exposure. Fiber aerosols contained 150-230 fibers/cc longer than 20 micrometer (>20 micrometer). On post-exposure Day 1, long-fiber lung burdens for the 6 test fibers were similar (12-16 x 10(5) fibers/lung >20 micrometer). After 1 year, the percentage of fibers >20 micrometer remaining in the lung was 0.04-10% for SVFs but 27% for amosite. Lung clearance weighted half-times (WT1/2) for fibers >20 micrometer were 6 days for MMVF34, 50-80 days for the other 4 SVFs, and >400 days for amosite. This study and 3 previous studies demonstrate a broad range of biopersistences for 19 different SVFs and 2 asbestos types. Ten of these fibers also have been (or are being) tested in chronic inhalation studies; in these studies, the very biopersistent fibers were carcinogenic (amosite, crocidolite, RCF1, MMVF32, and MMVF33), while the more rapidly clearing fibers were not (MMVF10, 11, 21, 22, and 34). These studies demonstrate the importance of biopersistence as an indicator of the potential pathogenicity of a wide range of fiber types.
Johns Manville Corporation, Littleton, Colorado 80162-5005, USA.
T W
TW
Hesterberg
G
G
Chase
C
C
Axten
W C
WC
Miller
R P
RP
Musselman
O
O
Kamstrup
J
J
Hadley
C
C
Morscheidt
D M
DM
Bernstein
P
P
Thevenaz
D000336:Aerosols; D000818:Animals; D017639:Asbestos, Amosite / Q000493:pharmacokinetics*; D005898:Glass; D007616:Kaolin; D008168:Lung / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D019016:Mineral Fibers; D008954:Models, Biological; D051381:Rats; D011916:Rats, Inbred F344; D013997:Time Factors
D000336:Aerosols; D019016:Mineral Fibers; C103119:RCF-1 fiber; C009753:fiberglass; D017639:Asbestos, Amosite; D007616:Kaolin