[Cyclin protein expression on malignant glioma cells].
Cyclins are key components of the cell cycle progression machinery. Immunohistochemical detection of cyclins in relation to cell position, DNA content, by multiparameter flow cytometry has provided new cell cycle studies. T98G, U373MG, and U251MG were scheduled expression of cyclin A, B1 which are maximal in late S and G2M phase cells. T98G was scheduled expression of cyclin E. being maximal in G1 and S phase cells, but U251MG, U373MG were unscheduled expression of cyclin E. The expression of cyclin D1 were not expressed a pattern. These result may mean that cyclin E in an important protein of cell cycle machinery of malignant brain tumor.
Dept. of Neurosurgery Kansai Medical University, Osaka, Japan. numa@takii.kmu.ac.jp
Y
Y
Numa
Y
Y
Tsukazaki
M
M
Yamamoto
T
T
Matsudaira
K
K
Kawamoto
D001932:Brain Neoplasms / Q000737:chemistry*; D002453:Cell Cycle; D019925:Cyclin A / Q000032:analysis; D019926:Cyclin B / Q000032:analysis; D056744:Cyclin B1; D019938:Cyclin D1 / Q000032:analysis; D019927:Cyclin E / Q000032:analysis; D016213:Cyclins / Q000032:analysis*; D005434:Flow Cytometry; D005910:Glioma / Q000737:chemistry*; D006801:Humans; D014407:Tumor Cells, Cultured
C534699:CCNB1 protein, human; D019925:Cyclin A; D019926:Cyclin B; D056744:Cyclin B1; D019927:Cyclin E; D016213:Cyclins; D019938:Cyclin D1