Differential fasciculation of follicular nerves for transferring specifically localized cues of the vibrissa rudiments to the central trigeminal sensory system in mice, as exploited with DiI and DiA labeling.
Vibrissa connections play a decisive role in setting the somatotopic coordinates in the trigeminal sensory system. Although previous studies have examined the development of peripheral patterning, certain questions are still in dispute, for example, the way vibrissa connections are structured, and the relationship between periphery and central organization, and ganglion cell organization. In order to fill the blanks left by previous studies, the extension of ganglionic branches and the formation of vibrissa connections were reexamined by using fluorescent carbocyanin dyes, DiI and DiA, during embryonic days 10 to 14 in mouse. Whole-mount preparations satisfactorily demonstrated the ganglionic fiber system, which allowed detailed analysis at both macroscopic and microscopic levels. We show here that the differential fasciculation of follicular nerves is the critical process for organizing vibrissa connections which modulate the initially extending fiber pattern. Follicular nerves developed by fine fibers arising from initially ordered root fascicles, so as to connect with vibrissa rudiments that developed on the facial prominences. During differential fasciculation, ganglion cells were segregated into distinct groups by the vibrissa connections, whereas central fiber terminals did not yet develop specific structures in the nuclear region. In the primary order of the trigeminal sensory system, vibrissa connections in the periphery were organized before those of the central structure. These results indicate that trigeminal ganglion cells have a critical binomial function in order to transfer the somatotopic relations among vibrissa rudiments into the topographic coordinates of the central system.
Yamakado
M
M
Department of Anatomy, Jichi Medical School, Tochigi, Japan.
Honzawa
K
K
Tsuchiya
H
H
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D051379:Mice; D008813:Mice, Inbred ICR; D018613:Microscopy, Confocal; D009411:Nerve Endings / Q000196:embryology* / Q000502:physiology / Q000648:ultrastructure; D011247:Pregnancy; D011726:Pyridinium Compounds; D012668:Trigeminal Ganglion / Q000196:embryology* / Q000502:physiology / Q000648:ultrastructure; D014738:Vibrissae / Q000294:innervation*
D005456:Fluorescent Dyes; D011726:Pyridinium Compounds; C076868:4-(4-dihexadecylaminostyryl)-N-methylpyridium