Digital imaging microscopy of living cells.
Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.
Rizzuto
R
R
Dept of Biomedical Sciences, University of Padova, Italy. rizzuto@civ.bio.unipd.it
Carrington
W
W
Tuft
R A
RA
D002468:Cell Physiological Phenomena; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*