Amplification of genomic DNA fragments of Sarcocystis muris (Apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase.
Parasites of the phylum Apicomplexa (Sporozoa) cause diseases such as malaria, toxoplasmosis, or intestinal coccidiosis. Invasive stages possess typical apical organelles such as dense granules that harbor a broad range of polypeptides that are believed to take part in the parasite-host cell interaction. In previous studies a 26-kDa polypeptide of dense granules from Sarcocystis muris cyst merozoites (bradyzoites) was characterized as a thiol (cysteine) proteinase. In this paper a method is demonstrated to amplify DNA fragments from genomic DNA of S. muris cyst merozoites by polymerase chain reaction, which probably code for the 26-kDa antigen.
Ruhr Universität Bochum, Arbeitsgruppe Molekulare Genetik, Germany.
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T
Hansner
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B
Freyer
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H
Mehlhorn
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W
Rüger
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D018503:Genome, Protozoan; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011487:Protein Conformation; D015800:Protozoan Proteins; D011994:Recombinant Proteins / Q000737:chemistry; D012522:Sarcocystis / Q000201:enzymology / Q000235:genetics*; D016415:Sequence Alignment
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D011994:Recombinant Proteins; D003546:Cysteine Endopeptidases; C114435:SmTP1 protein, Sarcocystis muris