Isolation of genomic DNA containing a cytosolic ascorbate peroxidase gene (ApxSC) from the strawberry (Fragaria x ananassa).
We isolated a genomic DNA harboring a cytosolic ascorbate peroxidase gene (ApxSC) from a genomic library of the strawberry (Fragaria x ananassa). Restriction mapping and sequence analyses showed that the DNA is composed of 2.5 kb of the full-length ApxSC gene, 3.7 kb of the 5'-upstream region, and 0.5 kb of the 3'-downstream region. The ApxSC genomic DNA contains 10 exons and 9 introns, which is similar to the structure of pea ApxI. A primer extension analysis suggested that the transcription of ApxSC gene was started at three start sites with different degrees. The promoter region of ApxSC gene contains a sequence or structure distinct from other reported plant ascorbate peroxidase genes, though with several known functional elements such as a TATA box.
Kim
I J
IJ
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Chung
W I
WI
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D003600:Cytosol / Q000201:enzymology; D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification*; D005638:Fruit / Q000201:enzymology*; D018745:Genome, Plant; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D018532:Pisum sativum / Q000201:enzymology; D010544:Peroxidases / Q000235:genetics*; D015183:Restriction Mapping; D014158:Transcription, Genetic / Q000235:genetics
D018744:DNA, Plant; D007527:Isoenzymes; D010544:Peroxidases; D060387:Ascorbate Peroxidases