T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens.
Prodigiosin was isolated from the culture broth of Serratia marcescens B-1231. This compound inhibited the T-cell mediated immune responses such as concanavalin-A induced proliferation, mixed lymphocyte response, local graft vs host reaction and T-dependent antibody response at non-toxic concentrations. However, prodigiosin did not affect B-cell mediated immune functions such as lipopolysaccharide-induced proliferation and-activated polyclonal antibody production at the same concentrations. Prodigiosin did not cause death in vitro to lymphocytes at effective concentrations (< 100 nM) and also did not show toxicity in vivo to lymphoid organs at effective dosages (10 and 30 mg/kg). The pharmacological potencies were comparable to the activities of other T-cell specific immunosuppressants such as cyclosporin A and FK-506. In conclusion, it might be suggested that prodigiosin could be used as an immunosuppressant in clinical and immunological studies.
Han
S B
SB
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusung, Taejon, Republic of Korea.
Kim
H M
HM
Kim
Y H
YH
Lee
C W
CW
Jang
E S
ES
Son
K H
KH
Kim
S U
SU
Kim
Y K
YK
D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D016131:Lymphocyte Subsets / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D011353:Prodigiosin / Q000302:isolation & purification* / Q000494:pharmacology*; D012706:Serratia marcescens / Q000737:chemistry* / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D010936:Plant Extracts; D011353:Prodigiosin