[Changes in the antioxidant system of the vitreous in rabbits after administration of sulfur hexafluoride].
OBJECTIVE
To evaluate the enzymatic activity of antioxidant system of rabbit's vitreous after sulfur hexafluoride (SF6) application.


METHODS
Activity of CuZn-SOD, catalase and concentration of MDA in fluid and gel fraction of vitreous were determined in 24 rabbits of New Zealand race on the 2nd, 7th and 14th day after SF6 application. Control group consisted of 6 animals which did not undergo any operations.


RESULTS
Dismutase and catalase activity as well as MDA concentration were higher in fluid fraction than in gel fraction in animals of control group. After SF6 application the activity of enzymes and MDA concentration did not change, whereas in fluid fraction all these values were statistically significantly reduced in all time intervals.


CONCLUSIONS
SF6 leads to disintegration of vitreous structure especially just after its application. Damage to hyalocytes causes dysfunction of enzymatic system. Specific fluid fraction structure and insufficient number of substrates for peroxidation processes are the reasons for simultaneous reduction of MDA concentration.
Kliniki Okulistycznej, Szpitala Klinicznego WAM w Lodzi.
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Sztarbała
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Goś
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Kedziora
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Błaszczyk
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Sibińska
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Góralczyk
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000032:analysis; D011817:Rabbits; D017382:Reactive Oxygen Species / Q000378:metabolism; D013459:Sulfur Hexafluoride / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014822:Vitreous Body / Q000187:drug effects* / Q000201:enzymology
D000975:Antioxidants; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D013459:Sulfur Hexafluoride