DNA extraction from Ascaris suum muscle tissue.
A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
Parasitologie, Institut de zoologie, Université de Neuchâtel, Switzerland.
C
C
Di Mito
B
B
Betschart
D000818:Animals; D017165:Ascaris suum / Q000235:genetics*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018509:DNA, Helminth / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D009132:Muscles / Q000737:chemistry; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction; D011205:Povidone
D002593:Cetrimonium Compounds; D018509:DNA, Helminth; D015246:Deoxyribonuclease EcoRI; D011205:Povidone; D000077286:Cetrimonium