Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.
The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.
Amersham International plc, Amersham Laboratories, Buckinghamshire, UK.
C L
CL
Smith
A C
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Simmonds
A L
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Hamilton
D L
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Martin
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Lashford
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Loakes
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Hill
D M
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Brown
D000906:Antibodies / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry; D003838:Deoxyadenine Nucleotides; D007211:Indoles / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis*
D000906:Antibodies; D015342:DNA Probes; D003838:Deoxyadenine Nucleotides; D007211:Indoles; D009841:Oligonucleotides; D004253:DNA Nucleotidylexotransferase; C026600:2'-deoxyadenosine triphosphate; C090067:5-nitroindole