Diversity and properties of calcium channel types in NG108-15 hybrid cells.
We used an integral of the current-voltage relation as a new evaluation of Ca2+ current component composition in NG108-15 hybrid cells. We determined significant changes in the values and composition of Ca2+ currents during cell differentiation. Only low-voltage-activated Ca2+ currents could be observed in undifferentiated cells; after cell differentiation, high-voltage-activated currents appeared and the total Ca2+ current was increased about 30-fold. By pharmacological and biophysical separation, we determined four main types of Ca2+ channels in differentiated cells: approximately 50%, 20% and 17% of N, T and L types, respectively, and 12% of residual current, which is insensitive to classical blockers of low- and high-voltage-activated currents, with the exception of (omega-conotoxin GVIA. All current components displayed kinetics and pharmacological properties similar to neuronal ones. We also established a significant Ca2+ dependence of omega-conotoxin GVIA to inhibit N-type Ca2+ channels: 10 mM Ca2+ in bath solution reduced the toxin efficacy to block N channels three-fold. The residual component fitted the properties of Q-type Ca2+ channels: it was sensitive to (omega-conotoxin GVIA and very similar to the T-type channel with respect to its kinetics; however, the threshold of its activation was closer to the high-voltage-activated component (- 40 mV). Our results show the functional diversity of Ca2+ channels and demonstrate, for the first time, that presumably the Q type of an alpha1A family, which has biophysical and pharmacological properties distinct from the previously described T, L and N types in these cells, is co-expressed in NG108-15 cells.
Royal Free Hospital School of Medicine, London, UK.
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Lukyanetz
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000145:classification / Q000187:drug effects / Q000502:physiology*; D002454:Cell Differentiation; D006822:Hybrid Cells; D015640:Ion Channel Gating; D051379:Mice; D018408:Patch-Clamp Techniques; D010455:Peptides; D051381:Rats; D020866:omega-Conotoxin GVIA
D002121:Calcium Channel Blockers; D015220:Calcium Channels; D010455:Peptides; D020866:omega-Conotoxin GVIA