Human trophoblast cells are permissive to the complete replicative cycle of human cytomegalovirus.
Human trophoblast cells were permissively infected by human cytomegalovirus. The kinetics of viral immediate-early, early, and late gene expression was clearly delayed compared to that in fibroblasts. Productive infection was unequivocally proven by the detection of virion particles, infectious virus in trophoblast culture supernatant, and cell-to-cell spread of cytomegalovirus from infected trophoblasts to uninfected fibroblasts. These observations indicate that infected trophoblasts may be involved in maternofetal transmission of human cytomegalovirus.
Department of Laboratory Medicine, University of Graz, A-8036 Graz, Austria.
G
G
Halwachs-Baumann
M
M
Wilders-Truschnig
G
G
Desoye
T
T
Hahn
L
L
Kiesel
K
K
Klingel
P
P
Rieger
G
G
Jahn
C
C
Sinzger
D000956:Antigens, Viral / Q000096:biosynthesis; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development / Q000502:physiology*; D006801:Humans; D014327:Trophoblasts / Q000821:virology*; D014779:Virus Replication
D000956:Antigens, Viral