Isolation and characterization of two proteins from Moraxella catarrhalis that bear a common epitope.
The UspA1 and UspA2 proteins of Moraxella catarrhalis are potential vaccine candidates for preventing disease caused by this organism. We have characterized both proteins and evaluated their vaccine potential using both in vitro and in vivo assays. Both proteins were purified from the O35E isolate by Triton X-100 extraction, followed by ion-exchange and hydroxyapatite chromatography. Analysis of the sequences of internal peptides, prepared by enzymatic and chemical cleavage of the proteins, revealed that UspA1 and UspA2 exhibited distinct structural differences but shared a common sequence including an epitope recognized by the monoclonal antibody 17C7. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), purified UspA1 exhibited a molecular weight of approximately 350, 000 when unheated and a molecular weight of 100,000 after being heated for 10 min at 100 degreesC. In contrast, purified UspA2 exhibited an apparent molecular weight of 240,000 by SDS-PAGE that did not change with the length of time of heating. Their sizes as determined by gel filtration were 1,150,000 and 830,000 for UspA1 and UspA2, respectively. Preliminary results indicate the proteins have separate functions in bacterial pathogenesis. Purified UspA1 was found to bind HEp-2 cells, and sera against UspA1, but not against UspA2, blocked binding of the O35E isolate to the HEp-2 cells. UspA1 also bound fibronectin and appears to have a role in bacterial attachment. Purified UspA2, however, did not bind fibronectin but had an affinity for vitronectin. Both proteins elicited bactericidal antibodies in mice to homologous and heterologous disease isolates. Finally, mice immunized with each of the proteins, followed by pulmonary challenge with either the homologous or a heterologous isolate, cleared the bacteria more rapidly than mock-immunized mice. These results suggest that UspA1 and UspA2 serve different virulence functions and that both are promising vaccine candidates.
McMichael
J C
JC
Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586-9728, USA. John_McMichael@internetmail.pr.cyanamid.com
Fiske
M J
MJ
Fredenburg
R A
RA
Chakravarti
D N
DN
VanDerMeid
K R
KR
Barniak
V
V
Caplan
J
J
Bortell
E
E
Baker
S
S
Arumugham
R
R
Chen
D
D
D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology; D000942:Antigens, Bacterial / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D003429:Cross Reactions; D004847:Epithelial Cells / Q000378:metabolism; D018985:Epitopes, B-Lymphocyte / Q000032:analysis*; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D008970:Molecular Weight; D001936:Moraxella catarrhalis / Q000276:immunology*; D016870:Neisseriaceae Infections / Q000276:immunology* / Q000517:prevention & control; D010455:Peptides; D017421:Sequence Analysis; D014407:Tumor Cells, Cultured; D019096:Vitronectin / Q000378:metabolism
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D018985:Epitopes, B-Lymphocyte; D005353:Fibronectins; D010455:Peptides; C089675:UspA protein, Moraxella catarrhalis; D019096:Vitronectin