Pancreatic trypsinogen I expression during cell growth and differentiation of two human colon carcinoma cells.
Pancreatic trypsin has been found to induce tight junction or dome formation in some colon cancer cell lines (HT-29, Caco-2), and a tumor-associated trypsinogen, trypsinogen type II, has been isolated from another colon cancer cell line (COLO 205). We have tried to determine if trypsinogen is present and how its expression varies during cell culture in HT-29 Glc+/- and Caco-2 cells, which exhibit enterocytic differentiation, and in HT-29 Glc+ cells, which never differentiate. Trypsinogen mRNA presence and expression were demonstrated in these cells by mRNA hybridization, RT-PCR, cytoimmunofluorescence, Western immunoblot analysis, and gel filtration. Trypsinogen was found to be trypsinogen type I and was mainly in zymogen form in culture media. Differentiating cells exhibited variations in trypsinogen I expression, but cells that remained undifferentiated did not. In the differentiated cells, a high and transient peak in trypsinogen I expression was observed during the first steps of differentiation.
Groupe de Recherche sur la Trophicité et le Vieillissement, Faculté de Médecine, Nice, France.
F
F
Bernard-Perrone
J
J
Carrere
W
W
Renaud
C
C
Moriscot
K
K
Thoreux
P
P
Bernard
A
A
Servin
D
D
Balas
F
F
Senegas-Balas
D000230:Adenocarcinoma / Q000201:enzymology* / Q000473:pathology; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D002850:Chromatography, Gel; D003110:Colonic Neoplasms / Q000201:enzymology* / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D006801:Humans; D009693:Nucleic Acid Hybridization; D010179:Pancreas / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D014362:Trypsinogen / Q000235:genetics*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D014362:Trypsinogen; D012194:RNA-Directed DNA Polymerase