Inhibition of icosanoid production in MC/9 mouse mast cells by n-3 polyunsaturated fatty acids isolated from edible marine algae.
The effects of two polyunsaturated fatty acids, 18:4n-3 and 16:4n-3 purified from the marine algae, Undaria pinnatifida and Ulva pertusa, on icosanoid production in MC/9 mouse mast cells were assessed. Both fatty acids suppressed the production of leukotriene B4 (LTB4), leukotriene C4 (LTC4), and 5-hydroxyeicosatetraenoic acid (5-HETE). The order of the suppressive activity for the two marine algae-derived fatty acids and three other common polyunsaturated fatty acids was as follows; 22:6n-3 = 18:4n-3 = 18:3n-3 > 20:5n-3 = 16:4n-3 for LTB4; 22:6n-3 = 18:4n-3 = 18:3n-3 > 16:4n-3 > 20:5n-3 (no suppression) for LTC4; 22:6n-3 = 18:4n-3 > 18:3n-3 > 20:5n-3 = 16:4n-3 for 5-HETE.
Marine Biochemistry Division, National Research Institute of Fisheries Science, Yokohama, Japan.
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K
Ishihara
M
M
Murata
M
M
Kaneniwa
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H
Saito
K
K
Shinohara
M
M
Maeda-Yamamoto
D000818:Animals; D000001:Calcimycin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D056890:Eukaryota / Q000737:chemistry*; D005231:Fatty Acids, Unsaturated / Q000302:isolation & purification* / Q000494:pharmacology; D006893:Hydroxyeicosatetraenoic Acids / Q000032:analysis*; D007476:Ionophores / Q000737:chemistry; D007975:Leukotriene B4 / Q000032:analysis*; D017997:Leukotriene C4 / Q000032:analysis*; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010945:Plants, Edible
D005231:Fatty Acids, Unsaturated; D006893:Hydroxyeicosatetraenoic Acids; D007476:Ionophores; C114465:hexadecatetraenoic acid; D007975:Leukotriene B4; D017997:Leukotriene C4; D000001:Calcimycin; C022022:5-hydroxy-6,8,11,14-eicosatetraenoic acid; C013641:parinaric acid