Detection of specific bacteriocin-producing lactic acid bacteria by colony hybridization.
A colony hybridization method for detecting lactic acid bacteria encoding specific bacteriocins was developed. Specific PCR-generated probes were used to detect colonies of pediocin PA-1, lactococcin A, enterocin AS-48, nisin A and lacticin 481 producing strains. The probes were shown to be sensitive and specific for sequences belonging to the structural genes of the respective bacteriocins.
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
M I
MI
Martínez
E
E
Rodríguez
M
M
Medina
P E
PE
Hernández
J M
JM
Rodríguez
D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D015342:DNA Probes; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D019344:Lactic Acid / Q000378:metabolism*; D015336:Molecular Probe Techniques; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D013289:Streptococcaceae / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
D001430:Bacteriocins; D015342:DNA Probes; D004269:DNA, Bacterial; D019344:Lactic Acid