Morphometric analysis of McCoy cells inoculated with cerebrospinal fluid from patients with rabies.
To demonstrate the potential of McCoy cells for the isolation of rabies virus from the cerebrospinal (CSF) fluid of a patient with a diagnosis of rabies, McCoy cells were inoculated with CSF from a patient with a clinical diagnosis of rabies and investigated in terms of morphometric aspect using the JAVA analysis system for the quantification of the increased size of infected cells compared to noninfected cells. The cells were also examined in terms of specific staining for the diagnosis of rabies by the method of Sellers for the observation of intracytoplasmic inclusions and by specific immunofluorescence staining for rabies virus. Infected cells showed changes in cell permeability and morphologic modifications which differed significantly compared to normal cells (P < 0.001) when analyzed by the Mann-Whitney and Kruskal-Wallis tests. Intense activity of the endoplasmic reticulum was also observed, as indicated by the presence of intracytoplasmic inclusions visualized by specific staining. The present study demonstrated the isolation of rabies virus from the CSF of a patient with rabies, showing that McCoy cells can be used for the laboratory diagnosis of patients suspected to have rabies.
Serviço de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil. ynogueir@usp.br
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D018929:Cell Culture Techniques; D002460:Cell Line / Q000821:virology; D048429:Cell Size; D002555:Cerebrospinal Fluid / Q000821:virology*; D006801:Humans; D011818:Rabies / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000473:pathology; D011820:Rabies virus / Q000302:isolation & purification*; D018709:Statistics, Nonparametric