Influence of substrate type and temperature on the developmental morphology of Pandora neoaphidis (Zygomycetes: entomophthorales), a pathogen of the tobacco aphid (Homoptera: aphididae).
Developmental morphology of Pandora neoaphidis was observed on the surfaces of the tobacco aphid, Myzus nicotianae, tobacco leaves (Nicotiana tabacum), and glass coverslips at 13 and 20 degrees C for 12 and 24 h postinoculation. Pandora neoaphidis responded similarly on the two living substrates, but differed on the inert coverslips. The proportions of ellipsoid conidia (primary and secondary) were similar on all substrates. Higher proportions of appressoria and lower proportions of round secondary conidia and germinating conidia occurred on the aphids and leaves than on the coverslips. Appressoria predominated over round secondary conidia and germinating conidia on the living substrates at 20 degrees C, but the opposite was seen at 13 degrees C. The proportions of ellipsoid conidia were similar at both temperatures. On coverslips, the proportions of appressoria and round secondary conidia were similar at both temperatures. However, the proportions of germinating and ellipsoid conidia were higher at 13 and 20 degrees C, respectively.
Dara
S K
SK
Southern Piedmont Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, Route 3, Blackstone, Virginia, 23824, USA. s.dara@cgnet.com
Semtner
P J
PJ
D000818:Animals; D001042:Aphids / Q000382:microbiology*; D004773:Entomophthora / Q000502:physiology / Q000648:ultrastructure*; D010947:Plants, Toxic; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana