Prokaryotic promoters in biotechnology.
The basic properties of prokaryotic promoters and the promotor region are described with special emphasis on promoters that are found in Escherichia coli and Bacillus subtilis. Promoters recognized by major and minor forms of RNA polymerase holoenzymes are compared for their specificities and differences. Both natural and hybrid promoters that have been constructed for purposes of efficient and regulated transcription are discussed in terms of their utility. Since promoter regions contain sequences that are recognized not only by RNA polymerase but by positive and negative regulatory factors that regulate expression from promoters, the functions and properties of these promoter regions are also described. The current utility and the future prospects of the prokaryotic promoters in expressing heterologous genes for biotechnology purposes are discussed.
Section of Plant Biology, University of California, Davis 95616, USA.
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D001412:Bacillus subtilis / Q000201:enzymology*; D001483:Base Sequence; D001709:Biotechnology / Q000379:methods*; D003090:Coliphages / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D012808:Sigma Factor; D012321:DNA-Directed RNA Polymerases