Endothelial cell damage and angiotensin-converting enzyme insertion/deletion genotype in elderly hypertensive patients.
OBJECTIVE
The purpose of this study was to investigate the angiotensin-converting enzyme (ACE) insertion/deletion (I/D) genotype and endothelial cell dysfunction or hypercoagulable state in elderly hypertensive patients.


BACKGROUND
Angiotensin-converting enzyme (ACE) insertion/ deletion (I/D) polymorphism was recently reported to be associated with various cardiovascular diseases. However, the precise mechanism of this association remains unknown, and some confounding factors might also affect the association. Endothelial cell dysfunction and coagulation activation play important roles in both the atherosclerotic process and the onset of cardiovascular events.


METHODS
We identified the ACE I/D genotype and measured the plasma levels of markers of endothelial cell damage (von Willebrand factor [vWF] and thrombomodulin) and of coagulation activation (prothrombin fragment F1 + 2 [F1 + 2]) in 318 asymptomatic elderly patients with hypertension, aged 59-93 years.


RESULTS
The vWF level was significantly higher in those with the DD genotype (n = 54) than in those with the II genotype (n = 131, p < 0.0001) or with the ID genotype (n = 133, p < 0.0001). The TM levels were also higher in patients with the ID genotype (p < 0.005) and the DD genotype (p < 0.01) than in those with the II genotype. There were no differences in F1 + 2 level among the groups. Positive correlations of systolic blood pressure with levels of both vWF and thrombomodulin were found predominantly in patients with the II genotype (both p < 0.001), but no correlation was noted in those with the DD genotype.


CONCLUSIONS
Considering the increased plasma levels of both endothelial cell-derived markers in the hypertensive patients with ACE DD genotype, we speculate that the ACE D allele is a risk factor for the development of hypertensive cardiovascular disease associated with endothelial cell damage.
Kario
K
K
Department of Cardiology, Jichi Medical School, Tochigi, Japan.
Matsuo
T
T
Kobayashi
H
H
Kanai
N
N
Hoshide
S
S
Mitsuhashi
T
T
Ikeda
U
U
Nishiuma
S
S
Matsuo
M
M
Shimada
K
K
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D001161:Arteriosclerosis / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D001777:Blood Coagulation / Q000235:genetics; D001794:Blood Pressure; D002318:Cardiovascular Diseases / Q000235:genetics; D015986:Confounding Factors, Epidemiologic; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology*; D005260:Female; D017353:Gene Deletion; D005838:Genotype; D006801:Humans; D006973:Hypertension / Q000201:enzymology* / Q000235:genetics; D008297:Male; D008875:Middle Aged; D016254:Mutagenesis, Insertional / Q000235:genetics*; D010446:Peptide Fragments / Q000032:analysis; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D011110:Polymorphism, Genetic / Q000235:genetics; D011498:Protein Precursors / Q000032:analysis; D011516:Prothrombin / Q000032:analysis; D012307:Risk Factors; D018180:Thrombomodulin / Q000097:blood; D014841:von Willebrand Factor / Q000032:analysis
D015415:Biomarkers; D010446:Peptide Fragments; D011498:Protein Precursors; D018180:Thrombomodulin; D014841:von Willebrand Factor; C029733:prothrombin fragment 1; C044183:prothrombin fragment 2; D011516:Prothrombin; D007703:Peptidyl-Dipeptidase A