Electrophoretic profile of hybrids between cryotolerant and non-cryotolerant Saccharomyces strains.
The chromosomal DNAs of cryotolerant Saccharomyces bayanus, non-cryotolerant Saccharomyces cerevisiae strains and their intra and interspecific hybrids were separated by pulsed field electrophoresis (PFGE). The cryotolerant and non-cryotolerant strains gave distinctly different electrophoretic profiles. The hybrids cryotolerant x cryotolerant and non-cryotolerant x non-cryotolerant were fertile and they gave the same electrophoretic karyotype as the respective parents. The cryotolerant x non-cryotolerant hybrids were sterile and gave electrophoretic karyotypes which showed both the bands the parents have in common and those they do not share.
Giudici
P
P
Istituto di Industrie Agrarie, University of Catania, Reggio Emilia, Italy.
Caggia
C
C
Pulvirenti
A
A
Zambonelli
C
C
Rainieri
S
S
D003080:Cold Temperature; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D006824:Hybridization, Genetic; D007621:Karyotyping; D012440:Saccharomyces / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D013045:Species Specificity
D004271:DNA, Fungal