Cloning and characterization of a microsatellite in the mitochondrial control region of the African side-necked turtle, Pelomedusa subrufa.
The nucleotide sequence of the African side-necked turtle mitochondrial control region and its flanking tRNA genes was determined. This 73% A+T-rich region is 1194 bp long. Several conserved motifs involved in the regulation of the mitochondrial genome replication process, including one conserved sequence block (CSB1), and three termination-associated sequences were identified. The most remarkable feature found in this control region was the presence of six microsatellite-containing tandem repeats between the CSB1 motif and the tRNAPhe gene. The potential usefulness of this microsatellite sequence for population-level studies is enhanced by its unique localization in the maternally inherited mitochondrial molecule.
Department of Ecology and Evolution and Program in Genetics, State University of New York, Stony Brook, NY 11794-5245, USA.
R
R
Zardoya
A
A
Meyer
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D005828:Genetics, Population; D018895:Microsatellite Repeats / Q000235:genetics*; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D017422:Sequence Analysis, DNA; D014426:Turtles / Q000235:genetics*
D004272:DNA, Mitochondrial; D005819:Genetic Markers