Use of a recombinant murine cytomegalovirus expressing vesicular stomatitis virus G protein to pseudotype retroviral vectors.
A new method of producing vesicular stomatitis virus (VSV) G protein pseudotyped retroviral vectors is described. In this method, stocks of VSV-G pseudotyped vector were reproducibly obtained by infecting an env-, human, retroviral vector producer cell line with a recombinant murine cytomegalovirus (CMV) which expresses VSV-G protein. The recombinant murine CMV, RMCMVG, expressed VSV-G protein under transcriptional control of the human CMV immediate-early promoter. RMCMVG, like murine CMV, can infect human cells, but the infection is limited to the expression of the viral immediate-early genes; no productive replication of murine CMV occurs. Recombinant murine CMV vector infection of non-permissive cells may be useful in situations where high levels of gene expression are desired without concomitant viral vector replication.
Manning
W C
WC
Chiron Corporation, Emeryville, CA 94608, USA. william_manning@cc.chiron.com
Murphy
J E
JE
Jolly
D J
DJ
Mento
S J
SJ
Ralston
R O
RO
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D018014:Gene Transfer Techniques; D005814:Genes, Viral / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D015726:Giant Cells; D006023:Glycoproteins / Q000096:biosynthesis / Q000235:genetics; D006801:Humans; D008562:Membrane Glycoproteins; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics* / Q000254:growth & development; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D011995:Recombination, Genetic; D012190:Retroviridae / Q000235:genetics* / Q000254:growth & development; D014162:Transfection; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000235:genetics*; D010948:Viral Plaque Assay
C028391:G protein, vesicular stomatitis virus; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins