The Crithidia fasciculata KAP1 gene encodes a highly basic protein associated with kinetoplast DNA.
The Crithidia fasciculata KAP1 gene encodes a small basic protein (p21) associated with kinetoplast DNA. The p21 protein has a nine amino acid cleavable presequence closely related to those of several other proteins targeted to the kinetoplast and binds non-specifically to kinetoplast minicircle DNA. The p21 protein also has a calculated pI of 13 with two amino acids (lysine and alanine) accounting for more than 50% of the residues and with 25 out of 28 lysine residues contained in the C-terminal half of the protein. Immunolocalization of p21 shows that the protein is found exclusively in the kinetoplast with a localization distinctly different from the antipodal localization of kinetoplast DNA topoisomerase and DNA polymerase. The KAP11 gene is a single copy gene and the KAP1 mRNA is present at a constant level throughout the cell cycle. This highly basic protein may play a role in the condensation or segregation of the kinetoplast DNA.
Molecular Biology Institute and Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles 90095-1570, USA.
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Hines
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D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D016831:Crithidia fasciculata / Q000737:chemistry / Q000235:genetics*; D018105:DNA, Kinetoplast / Q000235:genetics* / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D017125:Genes, Protozoan / Q000235:genetics*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014345:Trypanosoma / Q000235:genetics
D000913:Antibodies, Protozoan; D018105:DNA, Kinetoplast; D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; C114958:KAP1 protein, Crithidia fasciculata; D015800:Protozoan Proteins