Role of hydrogen peroxide in antitumor activity induction by sodium 5,6-benzylidene-L-ascorbate.
Role of hydrogen peroxide (H2O2) in the induction of antitumor activity against chemically-induced rat hepatocellular carcinoma by sodium 5,6-benzylidene-L-ascorbate (SBA) was investigated. ESR spectroscopy demonstrated that rat liver homogenate of cancerous tissue significantly enhanced the radical intensity of SBA more potently than that of precancerous or normal tissue. The peroxyoxalate chemiluminescence method demonstrated that SBA significantly enhanced the production of H2O2-derived chemiluminescence intensity in the liver homogenates, and the effect of SBA was greater in cancerous tissue than in precancerous or normal tissue. Addition of ascorbic acid, a degradation product of SBA, showed similar but slightly weaker stimulation effects. These data suggest that antitumor activity of SBA in vivo might, at least in part, be due to H2O2 production.
Sakagami
H
H
Department of Dental Pharmacology, Meikai University School of Dentistry, Saitama, Japan. sakagami@dent.meikai.ac.jp
Hosaka
M
M
Arakawa
H
H
Maeda
M
M
Satoh
K
K
Ida
Y
Y
Asano
K
K
Hisamitsu
T
T
Takimoto
M
M
Ota
H
H
Inagaki
M
M
Sasuga
K
K
Sho
S
S
Tanaka
T
T
Utsumi
N
N
Oi
T
T
Kochi
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D001597:Benzylidene Compounds / Q000627:therapeutic use*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D008163:Luminescent Measurements; D008297:Male; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D004124:p-Dimethylaminoazobenzene
D000970:Antineoplastic Agents; D001597:Benzylidene Compounds; D004124:p-Dimethylaminoazobenzene; D006861:Hydrogen Peroxide; D001205:Ascorbic Acid; C070680:zilascorb