Poly-(3-hydroxybutyrate) production from whey by high-density cultivation of recombinant Escherichia coli.
Recombinant Escherichia coli strain GCSC 6576, harboring a high-copy-number plasmid containing the Ralstonia eutropha genes for polyhydroxyalkanoate (PHA) synthesis and the E. coli ftsZ gene, was employed to produce poly-(3-hydroxybutyrate) (PHB) from whey, pH-stat fed-batch fermentation, using whey powder as the nutrient feed, produced cellular dry weight and PHB concentrations of 109 g l-1 and 50 g l-1 respectively in 47 h. When concentrated whey solution containing 210 g l-1 lactose was used as the nutrient feed, cellular dry weight and PHB concentrations of 87 g l-1 and 69 g l-1 respectively could be obtained in 49 h by pH-stat fed-batch culture. The PHB content was as high as 80% of the cellular dry weight. These results suggest that cost-effective production of PHB is possible by fed-batch culture of recombinant E. coli using concentrated whey solution as a substrate.
Department of Chemical Engineering, Advanced Institute of Science and Technology (KAIST), Taejon, Korea.
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Lee
D000421:Alcaligenes / Q000235:genetics; D000818:Animals; D002417:Cattle; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000737:chemistry* / Q000378:metabolism; D007700:Kinetics; D007785:Lactose / Q000737:chemistry; D008892:Milk / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry* / Q000378:metabolism; D000094122:Polyhydroxybutyrates
D004274:DNA, Recombinant; D006885:Hydroxybutyrates; D007785:Lactose; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates