Characterization of the genes encoding D-amino acid transaminase and glutamate racemase, two D-glutamate biosynthetic enzymes of Bacillus sphaericus ATCC 10208.
In Bacillus sphaericus and other Bacillus spp., D-amino acid transaminase has been considered solely responsible for biosynthesis of D-glutamate, an essential component of cell wall peptidoglycan, in contrast to the glutamate racemase employed by many other bacteria. We report here the cloning of the dat gene encoding D-amino acid transaminase and the glr gene encoding a glutamate racemase from B. sphaericus ATCC 10208. The glr gene encodes a 28. 8-kDa protein with 40 to 50% sequence identity to the glutamate racemases of Lactobacillus, Pediococcus, and Staphylococcus species. The dat gene encodes a 31.4-kDa peptide with 67% primary sequence homology to the D-amino acid transaminase of the thermophilic Bacillus sp. strain YM1.
Fotheringham
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IG
Biosciences Laboratory, NSC Technologies, Mt. Prospect, Illinois 60056, USA. igfoth@ccmail.monsanto.com
Bledig
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SA
Taylor
P P
PP
D000591:Amino Acid Isomerases / Q000235:genetics*; D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics*; D017124:Conserved Sequence; D051263:D-Alanine Transaminase; D018698:Glutamic Acid / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D000637:Transaminases / Q000235:genetics*
D001426:Bacterial Proteins; D018698:Glutamic Acid; D000637:Transaminases; D051263:D-Alanine Transaminase; D000591:Amino Acid Isomerases; C062292:glutamate racemase