An immunogenic epitope of Chlamydia pneumoniae from a random phage display peptide library is reactive with both monoclonal antibody and patients sera.
Random 15-mer peptides displayed on filamentous phages were screened in binding studies using a Chlamydia pneumoniae-specific monoclonal antibody (RR-402) and affinity-purified, polyclonal sera from patients seropositive for C. pneumoniae infections by the microimmunofluorescence (MIF) test. One 15-mer epitope, epitope Cpnl5A (LASLCNPKPSDAPVT) was identified in both the monoclonal and polyclonal screenings, and showed higher ELISA reactivity with C. pneumoniae MIF-positive sera compared to patients with other chlamydial infections, non-chlamydial respiratory infections and normal healthy sera (MIF-negative). Interestingly, epitope Cpnl5A also showed significant (52%) amino acid sequence homology to the 56 kDa type-specific antigen of Rickettsia tsutsugamushi, a protein implicated in the virulence of this organism.
Naidu
B R
BR
Institute of Postgraduate Studies and Research, University of Malaya, Kuala Lumpur, Malaysia.
Ngeow
Y F
YF
Wang
L F
LF
Chan
L
L
Yao
Z J
ZJ
Pang
T
T
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D001435:Bacteriophages; D002690:Chlamydia Infections / Q000097:blood / Q000276:immunology*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000276:immunology
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library; D010455:Peptides