A cosmid specific for sequences encoding a microtubule-associated protein, MAP1B, contains a polymorphic microsatellite and maps to bovine chromosome 20q14.
A clone for the bovine gene MAP1B was isolated from a bovine cosmid library with a probe obtained by cross-species PCR. The single positive cosmid clone was localized by FISH to chromosome region 20q14. Analysis of the cosmid revealed the presence of a microsatellite motif with a two-allele polymorphism detectable by PCR amplification of genomic DNA. However, the analysis of this polymorphism in a bovine family affected by SMA indicated absence of very close linkage of the phenotype for bovine SMA with MAP1B.
Swiss Federal Institute of Technology, ETH Zentrum, Zurich, Switzerland.
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Eggen
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Solinas-Toldo
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Fries
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003360:Cosmids; D018076:DNA, Complementary; D018900:Dinucleotide Repeats; D005260:Female; D008297:Male; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009133:Muscular Atrophy / Q000235:genetics; D010375:Pedigree; D011110:Polymorphism, Genetic
D018076:DNA, Complementary; D008869:Microtubule-Associated Proteins; C085284:microtubule-associated protein 1B