Identification of two novel palindromic regulatory elements in the murine interleukin-5 promoter.
Interleukin-5 has remarkable specificity for the eosinophil lineage. This fact, combined with the biological specificity of eosinophilia suggests tight and independent regulation of IL-5 expression. Here we report two novel palindromic regulatory elements (PRE) which contain positive regulatory motifs (PRM) that control transcription of the murine IL-5 gene. The first element, mPRE1-IL5 (-79 to -90) contains the mPRM1 at positions -87 to -89 which operates as a positive regulatory element with mutation of this motif resulting in a 64% decrease in gene activity. Gene expression was reduced by 67% when a similar mutation was introduced into the mPRM2 (-467 to -469) of mPRE2-IL5 (-459 to -470). Both elements specifically bind proteins from EL4-23 cell nuclear extracts forming constitutive DNA-protein complexes. EMSA experiments utilising mutated mPRE-IL5 oligonucleotides indicate that in both elements, the mPRMs are essential for protein binding.
Schwenger
G T
GT
Division of Molecular Immunology, TVW Telethon Institute for Child Health Research and the University of Western Australia, West Perth, Australia.
Mordvinov
V A
VA
Karlen
S
S
D'Ercole
M
M
Sanderson
C J
CJ
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D005809:Genes, Regulator; D015848:Interleukin-5 / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014162:Transfection
D017931:DNA Primers; D015848:Interleukin-5; D004247:DNA; D008156:Luciferases