Variation of penicillin acylase partition coefficient with phase volume ratio in poly(ethylene glycol)-sodium citrate aqueous two-phase systems.
The influence of phase volume ratio on partition and purification of penicillin acylase from Escherichia coli on poly(ethylene glycol)-sodium citrate aqueous two-phase systems was studied. In PEG 1000 systems both partition coefficients of the enzyme and total protein increased with decreasing phase volume ratio. However, in PEG 3350 containing NaCl, penicillin acylase follows a reverse trend, while total protein behaves in the same way. Implications for protein purification designs are discussed.
Marcos
J C
JC
Instituto de Biotecnologia e Química Fina (Polo de Braga), Universidade do Minho, Campus de Gualtar, Portugal.
Fonseca
L P
LP
Ramalho
M T
MT
Cabral
J M
JM
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002951:Citrates; D004926:Escherichia coli / Q000201:enzymology; D010399:Penicillin Amidase / Q000302:isolation & purification*; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D000077559:Sodium Citrate
D002951:Citrates; D000077559:Sodium Citrate; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D010399:Penicillin Amidase