An alpha/beta-HSQC-alpha/beta experiment for spin-state selective editing of IS cross peaks
A generalized version of the TROSY experiment allows the spin-state selective editing of the four multiplet components of 15N-1H cross peaks of amide groups in proteins into four different subspectra, with no penalty in sensitivity. An improvement by <SGMLEXP TYPE="INLINE">2 in sensitivity results, if only two of the four multiplet components are selected. Use of the experiment for the measurement of 1JHN coupling constants is discussed. A water flip-back version of the experiment is demonstrated with a 45 kDa fragment of 15N/2H labeled Staphylococcus aureus gyrase B. Copyright 1998 Academic Press.
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, S-171 77, Sweden.
P
Andersson
A
Annila
G
Otting