Porcine steroidogenic factor-1 gene (pSF-1) expression and analysis of embryonic pig gonads during sexual differentiation.
The porcine steroidogenic factor-1 gene (pSF-1) was cloned using a combination of genomic and RT-PCR based cloning methods. pSF-1 consists of an open reading frame of 1383 nt corresponding to a deduced amino acid sequence of 461 aa, similar to bovine and human SF-1. Sequence homologies between pSF-1 and human, bovine and mouse molecules indicate strong evolutionary conservation at both the nt and aa levels. Northern analysis of pSF-1 expression in adult steroidogenic tissues correlated with porcine steroidogenic acute regulatory protein gene (pStAR) and porcine side chain cleavage (pP450scc) gene expression. Notably, pSF-1 expression was readily detected in neonatal testes, absent at 3 weeks of age, and again readily detected at 3 months and in adult testes. pSF-1 expression was weak but detectable in placental tissues at various times of gestation, and was correlated with pStAR and pP450scc expression, indicating classical steroidogenesis in this organ. In developing gonads from 6-12 weeks of gestation, i.e. during the time of sex differentiation in the pig, Northern analysis demonstrated increasing expression of PSF-1 in fetal testes and no expression in ovaries. This expression pattern was paralleled for pStAR, pP450scc, and porcine Müllerian inhibitory substance (pMIS), consistent with pSF-1 involvement in both steroid and protein hormone secretions of the developing testes during sex differentiation. Porcine SRY HMG-box related gene-9 (pSOX-9) expression also paralleled that of pSF-1 in developing testes. In contrast, DSS-AHC critical region on the X chromosome, gene 1 (pDAX-1) was expressed predominantly in the developing ovaries, indicating a possible reciprocal regulation of pSF-1 and pDAX-1 genes in developing pig testes and ovaries.
Centre de Recherche en Reproduction Animale, Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montréal, St.-Hyacinthe, Québec, Canada.
N
N
Pilon
R
R
Behdjani
I
I
Daneau
J G
JG
Lussier
D W
DW
Silversides
D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D051717:Fushi Tarazu Transcription Factors; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006066:Gonads / Q000196:embryology* / Q000254:growth & development*; D018398:Homeodomain Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D018160:Receptors, Cytoplasmic and Nuclear; D017386:Sequence Homology, Amino Acid; D012733:Sex Differentiation / Q000502:physiology*; D054339:Steroidogenic Factor 1; D013552:Swine; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; C515057:NR5A1 protein, human; D018160:Receptors, Cytoplasmic and Nuclear; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; C515058:steroidogenic factor 1, mouse