Characterization of the UL55 gene product of herpes simplex virus type 2.
We have identified the herpes simplex virus type 2 (HSV-2) UL55 gene product using a rabbit polyclonal antiserum raised against a recombinant 6 x His-UL55 fusion protein expressed in Escherichia coli. The antiserum reacted specifically with a 23 kDa protein in HSV-2 186-infected cell lysates. The protein was not detectable in the presence of the viral DNA synthesis inhibitor phosphonoacetic acid. Indirect immunofluorescence studies localized the UL55 protein within and at the periphery of the nucleus as discrete granules at late times post-infection, and nuclear fractionation studies showed that the protein was associated with the nuclear matrix of infected cells. Moreover, these discrete regions containing the UL55 protein were found to be adjacent to compartments, designated assemblons, containing the capsid protein ICP35. However, the UL55 protein was not detected in purified virions. These results suggest that the UL55 protein of HSV-2 may play an accessory role in virion assembly or maturation.
Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Japan.
H
H
Yamada
Y M
YM
Jiang
S
S
Oshima
T
T
Daikoku
Y
Y
Yamashita
T
T
Tsurumi
Y
Y
Nishiyama
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D002213:Capsid / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D015870:Gene Expression; D018258:Herpesvirus 2, Human / Q000235:genetics / Q000502:physiology*; D006801:Humans; D015530:Nuclear Matrix / Q000378:metabolism; D011817:Rabbits; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D014771:Virion / Q000378:metabolism
D000914:Antibodies, Viral; D014764:Viral Proteins; C056094:herpes simplex virus type 2 protein ICP35