Overexpression of the PC3/TIS21/BTG2 mRNA is part of the stress response induced by acute pancreatitis in rats.
We have previously shown that the acute phase reaction of the pancreas is a powerful emergency mechanism which protects the organism against further pancreatic aggression. In an attempt to understand the mechanisms involved in this protective effect we tried to characterize at the molecular level the phenotypic changes of the pancreatic cell during acute stress. Using a systematic approach, we identified the PC3/TIS21/BTG2 mRNA as strongly overexpressed in pancreas during the acute phase of pancreatitis. PC3/TIS21/BTG2 mRNA is also overexpressed in liver and kidney during acute pancreatitis but not in the other tissues analyzed. In addition, PC3/TIS21/BTG2 mRNA is overexpressed in kidney after a 30-min ischemia. Since acute pancreatitis and kidney ischemia-reperfusion-induced injury were associated with apoptosis, and PC3/TIS21/BTG2 has an antiapoptotic activity, we speculate that this protein may play a role in the control of apoptosis progression in these tissues.
Klinikum Mannheim, Fakultät für Klinische Medizin der Universität Heidelberg, Mannheim, D-68167, Germany.
F
F
Fiedler
G V
GV
Mallo
H
H
Bödeker
V
V
Keim
J C
JC
Dagorn
J L
JL
Iovanna
D000208:Acute Disease; D000818:Animals; D017209:Apoptosis; D016282:Aspartic Acid Endopeptidases / Q000235:genetics*; D015152:Blotting, Northern; D015870:Gene Expression; D017874:Immediate-Early Proteins / Q000235:genetics*; D007511:Ischemia; D007668:Kidney / Q000098:blood supply / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000378:metabolism; D010195:Pancreatitis / Q000378:metabolism*; D043484:Proprotein Convertases; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015424:Reperfusion
D017874:Immediate-Early Proteins; D012333:RNA, Messenger; D043484:Proprotein Convertases; D016282:Aspartic Acid Endopeptidases