Detection of factor V Leiden mutation in severe pre-eclamptic Hungarian women.
Pre-eclampsia is a pregnancy-related disorder that complicates approximately 5% of all pregnancies and is cited as the primary cause of worldwide maternal and fetal mortality. The factor V Leiden mutation has been implicated in the development of severe pre-eclampsia. In order to investigate this association, a sample of 198 Hungarian women was recruited and enrolled in one of the three groups based on reproductive and health status: those as classified as pregnant and healthy (n = 71), those diagnosed as pregnant and severe pre-eclamptic (n = 69), and those found to be healthy and non-pregnant (n = 58). The presence of factor V Leiden mutation was determined by using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis (RFLP). We identified three (5.2%) heterozygous among healthy non-pregnant participants, five (7.0%) heterozygous among healthy pregnant participants and 13 (18.8%) heterozygous among pre-eclamptic women (p < 0.05, 95% CI). Our result supports the previous observation that carriers of factor V Leiden mutation are at increased risk for developing severe pre-eclampsia.
1st Department of Obstetrics and Gynecology, Semmelweis University Medical School, Budapest, Hungary.
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Nagy
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Tóth
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Rigó
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Karádi
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Romics
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D000293:Adolescent; D000328:Adult; D005165:Factor V / Q000235:genetics*; D005260:Female; D006801:Humans; D006814:Hungary; D008875:Middle Aged; D009154:Mutation; D011225:Pre-Eclampsia / Q000235:genetics*; D011247:Pregnancy; D011248:Pregnancy Complications
C095381:factor V Leiden; D005165:Factor V