The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses.
DF-1 is a continuous cell line of chicken embryo fibroblasts. The cells are free of endogenous sequences related to avian sarcoma and leukosis viruses and have normal fibroblastic morphology. DF-1 cells support the replication of avian retroviruses; diverse oncogenes induce foci of oncogenic transformation on monolayers of DF-1, and avian leukosis viruses of envelope subgroups B, D, and C induce cell death and form plaques. The new cell line will greatly facilitate studies on oncogenic transformation and cell killing by avian viruses.
Himly
M
M
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California, 92037, USA.
Foster
D N
DN
Bottoli
I
I
Iacovoni
J S
JS
Vogt
P K
PK
D000818:Animals; D001354:Avian Leukosis Virus / Q000502:physiology*; D016923:Cell Death; D002460:Cell Line / Q000502:physiology* / Q000821:virology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology; D016758:Genes, jun / Q000502:physiology; D009857:Oncogenes / Q000502:physiology*; D014779:Virus Replication