Engineering of an anti-steroid antibody: amino acid substitutions change antibody fine specificity from cortisol to estradiol.
Immunoassays are widely used for determination of the concentration of steroid hormones. However, obtaining specific anti-steroid monoclonal antibodies remains difficult. We used antibody engineering and phage display methods to change the specificity of an anti-cortisol monoclonal antibody towards estradiol. This work demonstrates that production of recombinant antibodies may be a valuable way of obtaining the high-specificity antibodies required for steroid immunoassays.
Laboratoire d'Ingénierie des Systèmes Macromoléculaires, Marseille, France.
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D000305:Adrenal Cortex Hormones / Q000276:immunology*; D019943:Amino Acid Substitution; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D000918:Antibody Specificity; D001483:Base Sequence; D003429:Cross Reactions; D017931:DNA Primers; D004958:Estradiol / Q000276:immunology*; D006854:Hydrocortisone / Q000276:immunology*; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology
D000305:Adrenal Cortex Hormones; D000911:Antibodies, Monoclonal; D017931:DNA Primers; D011994:Recombinant Proteins; D004958:Estradiol; D006854:Hydrocortisone