Heat shock alters poly(A) tail length of Dictyostelium discoideum hsp32 RNA.
Hsp32 is a heat shock gene in D. discoideum. We have previously observed that heat stress-induced change produces a broad band on Northern blots, suggesting that more than one population of mRNA is present under those conditions. This was not the result of a defect in the splicing of the hsp32 mRNA, nor did it result from the use of a different transcription start site under heat shock conditions. Here, we show that the broad banding pattern reflects the appearance of a transcript with a poly(A) tail that is approximately 100 nt longer than that seen in unstressed cells. Experiments indicated that this tail was not a property of newly synthesized mRNA but rather a response to heat stress. This response appeared to be specific to the hsp32 transcript and did not result in the retention of the RNA in the nucleus. These results document a relatively unusual heat shock response and also indicate that the nature of the response differs among RNAs and has selective consequences.
Moerman
A M
AM
E.A. Doisy Department of Biochemistry and Molecular Biology, St. Louis University School of Medicine, MO 63104, USA.
de Maria
A C
AC
Gomes
S L
SL
Klein
C
C
D000818:Animals; D003470:Culture Media / Q000494:pharmacology; D004023:Dictyostelium / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005057:Eukaryotic Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000187:drug effects / Q000235:genetics*; D018869:Heat-Shock Response / Q000235:genetics*; D011061:Poly A / Q000235:genetics / Q000378:metabolism*; D015800:Protozoan Proteins; D012313:RNA / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D006360:Heat-Shock Proteins; C107554:Hsp32 protein, Dictyostelium discoideum; D015800:Protozoan Proteins; D012333:RNA, Messenger; D011061:Poly A; D012313:RNA