Rapid and easy method to extract and preserve DNA from Cryptococcus neoformans and other pathogenic yeasts.
The mucopolysaccharide capsule of Cryptococcus neoformans and other pathogenic yeasts prevent the extraction of DNA from these important zoonotic agents. We report that the use of a lysis buffer containing a high concentration of urea is an easy, efficient and time-saving technique to obtain high yields of good-quality DNA for molecular diagnosis. The use of urea also prevents the degradation of DNA during storage of samples at room temperature for up to 6 months.
Unidad Docente de Genética y Mejora Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
M E
ME
Sansinforiano
J A
JA
Padilla
J
J
Hermoso de Mendoza
M
M
Hermoso de Mendoza
J L
JL
Fernandez-Garcia
M
M
Martínez-Tranćon
A
A
Rabasco
J C
JC
Parejo
D003455:Cryptococcus neoformans / Q000737:chemistry*; D004271:DNA, Fungal / Q000302:isolation & purification*; D013048:Specimen Handling
D004271:DNA, Fungal