Further validation of antibody-capture and antigen-capture enzyme-linked immunosorbent assays for determining exposure of cattle to bovine coronavirus.
OBJECTIVE
To further validate an antibody-capture ELISA for measuring bovine coronavirus (BCV) exposure (antibody seroresponse) in cattle and to explain the apparent loss of sensitivity of a BCV antigen-capture ELISA when testing feces from adult versus neonatal cattle.


METHODS
98 adult cows from herds with and without winter dysentery; 10 gnotobiotic or colostrum-deprived calves.


METHODS
Results of an antibody-capture ELISA for BCV and a plaque reduction virus neutralization assay performed on paired serum samples from 24 cattle were compared with each other and with results of immunoelectron microscopy (IEM) of feces for BCV. For samples from 98 cattle, results of antibody-capture ELISA were compared with results of IEM. Calves were inoculated with feces ELISA-positive or IEM-positive for BCV and monitored for BCV infection. An ELISA was developed to detect BCV antigen-antibody complexes in feces and results were compared with results of an antigen-capture ELISA and IEM.


RESULTS
Antibody-capture ELISA results correlated with neutralization assay results, but agreed more closely with results of IEM. Calves became infected with BCV following inoculation with either ELISA-positive or ELISA-negative but IEM-positive feces. Results of the antigen-antibody ELISA correlated with results of IEM and the antigen-capture ELISA.


CONCLUSIONS
In adult cattle, testing of paired serum samples by use of an antibody-capture ELISA may be a better indicator of recent BCV exposure than results of virus neutralization tests. Antigen-antibody binding in feces may interfere with results of antigen-capture ELISA for BCV.
Ohio Agricultural Research and Development Center, Ohio State University, Wooster 44691, USA.
D R
DR
Smith
P R
PR
Nielsen
K L
KL
Gadfield
L J
LJ
Saif
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000936:Antigen-Antibody Complex / Q000032:analysis; D000956:Antigens, Viral / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D018352:Coronavirus Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D017938:Coronavirus, Bovine; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D009500:Neutralization Tests / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral