Anticholinesterase induces nicotinic receptor modulation.
The effects of carbamate anticholinesterases, pyridostigmine and physostigmine, on the function of the nicotinic receptor (nAChR) in TE671 cells was studied, precluding their inhibition of acetylcholine hydrolysis by carbachol usage. In radioassay, the simultaneous application of carbachol and carbamates dose-dependently decreased carbachol-induced 22Na+ influx, compared with carbachol activation alone. Increasing cell preincubation in the presence of carbamates, however, potentiated influx at low concentrations in a time-dependent manner. This facilitating effect of carbamates, even at high concentrations, was significantly increased by washing out these drugs and was blocked by pretreatment with diisopropylfluorophosphate. Similar results were also obtained in whole-cell patch-clamp study. There were insignificant changes in desensitization properties during facilitation. It is thus supposed that facilitation cannot be explained by the inhibition of acetylcholine hydrolysis. These results support a previous hypothesis that acetylcholinesterase might modulate nAChR by an unknown mechanism. In addition, the clinical effects of carbamates may be partly attributed to this facilitation.
Sung
J J
JJ
Department of Pharmacology, Neuroscience Research Institute, Seoul, Korea.
Kim
S J
SJ
Lee
H B
HB
Chung
J M
JM
Choi
Y M
YM
Cha
C I
CI
Suh
Y H
YH
Lee
K W
KW
D002217:Carbachol / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology*; D004594:Electrophysiology; D006801:Humans; D007531:Isoflurophate / Q000494:pharmacology; D011978:Receptors, Nicotinic / Q000187:drug effects* / Q000502:physiology*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D002219:Carbamates; D002800:Cholinesterase Inhibitors; D011978:Receptors, Nicotinic; D007531:Isoflurophate; D002217:Carbachol