Productive infection of Epstein-Barr virus (EBV) in EBV-genome-positive epithelial cell lines (GT38 and GT39) derived from gastric tissues.
We characterized the expression of Epstein-Barr virus (EBV) on two epithelial cell lines, GT38 and GT39, derived from human gastric tissues. The EBV nuclear antigen (EBNA) was detected in all cells of both cell lines. The EBV immediate-early BZLF 1 protein (ZEBRA), the early antigen diffuse component (EA-D), and one of the EBV envelope proteins (gp350/220) were expressed spontaneously in small proportions in the cells. EBNA 1, EBNA2, latent membrane protein 1, ZEBRA, and EA-D molecules were then observed by Western blotting in the cells. The lytic cycle was enhanced with treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) or n-butyrate. The virus particles were observed in the TPA treated GT38 cells by electron microscopy. Infectious EBV was detected with the transformation of cord blood lymphocytes and also with the induction of early antigen to Raji cells by the supernatants of both cells lines. A major single and minor multiple fused terminal fragments and a ladder of smaller fragments of the EBV genome were detected with a Xhol probe in both cell lines. These epithelial cells lines and viruses will be useful in studying their association with EBV in gastric epithelial cells.
Takasaka
N
N
Department of Biosignaling, Faculty of Medicine, Tottori University, Yonago, Japan.
Tajima
M
M
Okinaga
K
K
Satoh
Y
Y
Hoshikawa
Y
Y
Katsumoto
T
T
Kurata
T
T
Sairenji
T
T
D000818:Animals; D002087:Butyrates / Q000494:pharmacology; D020148:Butyric Acid; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism; D004847:Epithelial Cells / Q000648:ultrastructure / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005838:Genotype; D006566:Herpesviridae Infections / Q000821:virology; D004854:Herpesvirus 4, Human / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D006801:Humans; D013270:Stomach / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D013274:Stomach Neoplasms / Q000821:virology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015534:Trans-Activators / Q000378:metabolism; D014412:Tumor Virus Infections / Q000821:virology; D014764:Viral Proteins; D014779:Virus Replication
C063676:BZLF1 protein, Herpesvirus 4, Human; D002087:Butyrates; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D015534:Trans-Activators; D014764:Viral Proteins; D020148:Butyric Acid; D013755:Tetradecanoylphorbol Acetate