Negative regulation of the anti-human immunodeficiency virus and chemotactic activity of human stromal cell-derived factor 1alpha by CD26/dipeptidyl peptidase IV.
Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory activity of SDF-1alpha against HIV infection disappeared. Moreover, the chemotactic activity of SDF-1alpha also disappeared specifically by DPPIV activity of recombinant soluble CD26. These results suggested that dissemination of T-tropic HIV strains in vivo may be facilitated by CD26/DPPIV via inactivation of functional SDF-1alpha.
Department of Clinical Immunology and AIDS Research Center, Institute of Medical Science, University of Tokyo, Japan.
T
T
Ohtsuki
O
O
Hosono
H
H
Kobayashi
Y
Y
Munakata
A
A
Souta
T
T
Shioda
C
C
Morimoto
D000818:Animals; D000998:Antiviral Agents / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D054377:Chemokine CXCL12; D019743:Chemokines, CXC / Q000378:metabolism / Q000502:physiology*; D002630:Chemotactic Factors / Q000378:metabolism / Q000502:physiology*; D002633:Chemotaxis; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism / Q000494:pharmacology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D019169:Jurkat Cells; D007739:L Cells; D007963:Leukocytes, Mononuclear / Q000502:physiology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology
D000998:Antiviral Agents; C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C515256:Cxcl12 protein, mouse; D018819:Dipeptidyl Peptidase 4