Effect of cell swelling on membrane and cytoplasmic distribution of pICln.
pICln is found ubiquitously in mammalian cells and is postulated to play a critical role in cell volume regulation. Mutagenesis studies led to the proposal that pICln is a swelling-activated anion channel. However, recent studies in Madin-Darby canine kidney cells and endothelial cells have shown that the protein is localized primarily to the cytoplasm. It has therefore been postulated that activation involves reversible translocation of pICln from the cytoplasm and insertion into the plasma membrane. We tested this hypothesis using several different approaches. Fractionation of C6 glioma cells into plasma membrane- and cytoplasm-containing fractions demonstrated that approximately 90% of the recovered pICln was confined to the cytosol. Swelling had no effect on the relative amount of protein present in the plasma membrane fraction. Immunofluorescence microscopy revealed that pICln is localized primarily, if not exclusively, to the cytoplasm of swollen and nonswollen cells. Similarly, transfection of cells with a green fluorescent protein-labeled pICln construct failed to reveal any membrane localization of the protein. These findings do not support the hypothesis that pICln is a volume regulatory anion channel activated by swelling-induced membrane insertion.
Emma
F
F
Renal Division, Children's Hospital, Boston, Massachusetts 02115, USA.
Breton
S
S
Morrison
R
R
Wright
S
S
Strange
K
K
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D048429:Cell Size / Q000502:physiology*; D018118:Chloride Channels / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D007473:Ion Channels; D009997:Osmotic Pressure; D051381:Rats; D011993:Recombinant Fusion Proteins; D013347:Subcellular Fractions; D014162:Transfection; D014407:Tumor Cells, Cultured
D018118:Chloride Channels; C498729:Clns1a protein, rat; D007473:Ion Channels; D011993:Recombinant Fusion Proteins