Ultrasmall immunogold particles: important probes for immunocytochemistry.
In this article, we review the immunocytochemical literature with respect to a comparison between conventional colloidal gold and ultrasmall gold particles as immunoprobes. We discuss the relative advantages and disadvantages of each of these types of particles for immunocytochemical applications. We present results from our own laboratories, in which we compared these immunoprobes in selected experimental situations. In addition, we discuss our work on the use of a fluorescently labeled ultrasmall immunoprobe for correlative microscopy.
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus 43210, USA.
J M
JM
Robinson
T
T
Takizawa
D D
DD
Vandré
R W
RW
Burry
D005456:Fluorescent Dyes; D006801:Humans; D007089:Image Enhancement; D007140:Immunoglobulin Fab Fragments; D007150:Immunohistochemistry / Q000379:methods*; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods; D008870:Microtubules / Q000648:ultrastructure; D009504:Neutrophils / Q000648:ultrastructure; D012834:Silver
D005456:Fluorescent Dyes; D007140:Immunoglobulin Fab Fragments; D012834:Silver