Analysis of a Caenorhabditis elegans Twist homolog identifies conserved and divergent aspects of mesodermal patterning.
Mesodermal development is a multistep process in which cells become increasingly specialized to form specific tissue types. In Drosophila and mammals, proper segregation and patterning of the mesoderm involves the bHLH factor Twist. We investigated the activity of a Twist-related factor, CeTwist, during Caenorhabditis elegans mesoderm development. Embryonic mesoderm in C. elegans derives from a number of distinct founder cells that are specified during the early lineages; in contrast, a single blast cell (M) is responsible for all nongonadal mesoderm formation during postembryonic development. Using immunofluorescence and reporter fusions, we determined the activity pattern of the gene encoding CeTwist. No activity was observed during specification of mesodermal lineages in the early embryo; instead, the gene was active within the M lineage and in a number of mesodermal cells with nonstriated muscle fates. A role for CeTwist in postembryonic mesodermal cell fate specification was indicated by ectopic expression and genetic interference assays. These experiments showed that CeTwist was responsible for activating two target genes normally expressed in specific subsets of nonstriated muscles derived from the M lineage. In vitro and in vivo assays suggested that CeTwist cooperates with the C. elegans E/Daughterless homolog in directly activating these targets. The two target genes that we have studied, ceh-24 and egl-15, encode an NK-2 class homeodomain and an FGF receptor (FGFR) homolog, respectively. Twist activates FGFR and NK-homeodomain target genes during mesodermal patterning of Drosophila and similar target interactions have been proposed to modulate mesenchymal growth during closure of the vertebrate skull. These results suggest the possibility that a conserved pathway may be used for diverse functions in mesodermal specification.
Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210 USA.
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Harfe
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Vaz Gomes
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Kenyon
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Liu
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Krause
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Fire
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019521:Body Patterning; D017173:Caenorhabditis elegans / Q000196:embryology*; D029742:Caenorhabditis elegans Proteins; D019281:Dimerization; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000502:physiology; D008648:Mesoderm / Q000502:physiology*; D008969:Molecular Sequence Data; D009132:Muscles / Q000196:embryology*; D009687:Nuclear Proteins / Q000737:chemistry / Q000502:physiology*; D011401:Promoter Regions, Genetic; D017468:Receptors, Fibroblast Growth Factor / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000502:physiology; D051879:Twist-Related Protein 1
D029742:Caenorhabditis elegans Proteins; C111093:Ceh-24 protein, C elegans; C112493:EGL-15 protein, C elegans; C497752:HLH-8 protein, C elegans; D018398:Homeodomain Proteins; C081538:Mab-5 protein, C elegans; D009687:Nuclear Proteins; D017468:Receptors, Fibroblast Growth Factor; D014157:Transcription Factors; D051879:Twist-Related Protein 1