The use of reverse transcriptase-polymerase chain reaction to investigate environmental samples for the presence of enteroviruses.
Molecular techniques developed for detection and diagnosis of enteric viruses have been adapted for use with water samples. Although problems of interference and sensitivity remain, reliable and consistent methods should soon be available for the detection in water of enteroviruses, Norwalk-like viruses, and rotavirus.
Reading Public Health Laboratory, Pathology Laboratory, Royal Berkshire Hospital, Reading. jane@sellwood.demon.co.uk
J
J
Sellwood
J
J
Shore
S
S
Read
P
P
Wyn-Jones
D004770:Enterovirus / Q000302:isolation & purification*; D006801:Humans; D009663:Norwalk virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012401:Rotavirus / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014871:Water Microbiology