Cloning and expression analysis of a cDNA coding for a dexamethasone-inducible cytochrome P450 in Xenopus laevis liver.
We previously purified a cytochrome P450 (P450) from liver microsomes of adult female Xenopus laevis. In this study, we screened a cDNA library of Xenopus liver to isolate the cDNA clone coding for this P450. The 5'-end of the resultant cDNA was truncated at the N-terminal region and extended by method of rapid amplification of cDNA end to give the complete coding sequence. Amino acid sequence showed this clone to be 36% to 55% identical to members of the CYP2 family and less than 31% identical to members of other gene families, and to belong to the CYP2Q subfamily. This gene is expressed constitutively in the livers of adult male and female frogs, and is significantly induced by dexamethasone administration.
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
H
H
Ohi
E
E
Sugata
Y
Y
Fujita
H
H
Saito
K
K
Saguchi
N
N
Murayama
S
S
Higuchi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014982:Xenopus laevis
D018076:DNA, Complementary; D012333:RNA, Messenger; D003907:Dexamethasone; D003577:Cytochrome P-450 Enzyme System