New blocking method for the hydroxyl group on carbohydrate. Determination of the O-acylated position of the modified glycolipid.
To determine O-esterified positions, a rapid and complete acetalization to prepare an intermediate was established using ethyl vinyl ether as a new reagent. The new method was applied to O-esterified glycolipids followed by GC-MS analysis of the monosaccharide derivatives after methylation and methanolysis, revealing the derivatives with correctly substituted positions. This method was superior in terms of its shorter reaction time and complete acetalization, particularly of the N-glycolyl hydroxyl residue, to previously reported methods using methyl vinyl ether.
Department of Chemistry, Sapporo Medical University School of Medicine, Japan.
Y
Y
Yachida
K
K
Tsuchihashi
S
S
Gasa
M
M
Kashiwagi
T
T
Daino
T
T
Mikami
T
T
Akino
D000107:Acetylation; D000215:Acylation; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D006017:Glycolipids / Q000032:analysis*; D006900:Hydroxylation; D000432:Methanol; D008745:Methylation; D008969:Molecular Sequence Data
D002241:Carbohydrates; D006017:Glycolipids; D000432:Methanol