Modulation of erythrocyte photohemolysis rate by glutathione reductase inactivating alkylating agents.
In order to determine the role of glutathione reductase (GR) in protection against Alphtalocyanine tetrasulfonate-sensitized human erythrocyte photolysis, we have studied the effects of antitumour alkylating agents that inactivate GR, on photohemolysis rate. The rates of inactivation of reduced GR decreased in order BCNU > pharanox (N-p-[bis-(2-chloroethyl)-amino]-phenylacetic acid N-oxide) > phenalol (N-p-[bis-(2-chloroethyl)-amino]-phenylacetyl-L- phenylalanine) > o-F- and p-F-lophenal (o- and p-isomers of N-p-[bis-(2-chloroethyl)-amino]-phenylacetyl-D,L-fluorophenylalanine) > D,L-melphalan. As supposed, erythrocyte photolysis was accelerated by BCNU and pharanox, however, it was slowed down by phenylalanine mustards. The latter effect was explained by singlet oxygen quenching and/or photooxidation reactions of these compounds. This points out to a possibility of certain phenylalanine derivatives to neutralize the side-effects of photodynamic therapy.
Oncological Center, Santariskiu, Vilnius.
R
R
Kersienè
A
A
Marozienè
R
R
Kliukienè
Z
Z
Anusevicius
J
J
Didziapetrienè
N
N
Cènas
D000477:Alkylating Agents / Q000494:pharmacology*; D002330:Carmustine / Q000494:pharmacology; D005980:Glutathione Reductase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008027:Light; D008558:Melphalan / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism; D010101:Oxygen Consumption; D010648:Phenylacetates / Q000494:pharmacology; D010782:Photolysis; D017319:Photosensitizing Agents / Q000494:pharmacology; D026082:Singlet Oxygen; D008063:Thioctic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D000477:Alkylating Agents; D010648:Phenylacetates; D017319:Photosensitizing Agents; C113657:lophenal; C113659:pharanox; D026082:Singlet Oxygen; D008063:Thioctic Acid; C013091:lipoamide; D005980:Glutathione Reductase; D008558:Melphalan; D010100:Oxygen; D002330:Carmustine