Insulin treatment (hormonal imprinting) increases the insulin production of the unicellular tetrahymena long term. Is there a simultaneous formation of hormone receptor and hormone?
Insulin treatment of Tetrahymena pyriformis resulted in a long-lasting increase in the insulin content of the cells. After about 200 generations, insulin levels significantly higher than in the control were demonstrated by using a quantitative immunocytochemical method. Although the insulin content fluctuated from day to day, it was always higher in the insulin pre-treated (imprinted) cells than in the controls. The results emphasise the simultaneous stimulation of hormone and receptor formation by hormonal imprinting.
Department of Biology, Semmelweis University of Medicine, Budapest, Hungary.
G
G
Csaba
P
P
Kovács
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000494:pharmacology; D007328:Insulin / Q000096:biosynthesis / Q000276:immunology / Q000494:pharmacology*; D015800:Protozoan Proteins / Q000378:metabolism; D011972:Receptor, Insulin / Q000378:metabolism*; D013769:Tetrahymena pyriformis / Q000378:metabolism*
D007004:Hypoglycemic Agents; D007074:Immunoglobulin G; D007328:Insulin; D015800:Protozoan Proteins; D011972:Receptor, Insulin