Stability of lipid/DNA complexes during agitation and freeze-thawing.
It is well established that cationic liposomes facilitate the delivery of DNA and offer substantial advantages over viral-based delivery systems. However, these synthetic vectors readily aggregate in liquid formulations which in clinical trials requires preparation of lipid/DNA complexes at the bedside immediately before injection. This temporal requirement could be eliminated if complexes were formulated as stable preparations that could be shipped, stored, and administered as needed. To this end, our study investigates the stability of lipid/DNA complexes during physical stresses that might be encountered during shipping and storage, i.e., agitation and freeze-thawing. Our data show that agitation significantly reduces transfection rates in complexes prepared with three different commercially available lipid formulations. Additional experiments indicate that slow freezing is more damaging than rapid freezing, and that sucrose is able to preserve transfection and complex size during freeze-thawing. These results are consistent with previous reports and demonstrate that frozen formulations may be suitable for maintaining transfection rates of lipid/DNA complexes. Under certain conditions, we observe a reproducible 3-fold increase in transfection after freeze-thawing that is prevented by high concentrations of sucrose. Together, these data suggest that physical stresses can alter structural characteristics of lipid/DNA complexes that can markedly affect rates of DNA delivery.
Department of Pharmaceutical Sciences, School of Pharmacy and Center for Pharmaceutical Biotechnology, University of Colorado Health Sciences Center, 4200 E. Ninth Avenue, Denver, Colorado 80262, USA. anchordt@essex.uchsc.edu
T J
TJ
Anchordoquy
L G
LG
Girouard
J F
JF
Carpenter
D J
DJ
Kroll
D000818:Animals; D019556:COS Cells; D002411:Cation Exchange Resins / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004355:Drug Stability; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D005615:Freezing; D008055:Lipids / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D014162:Transfection
D002411:Cation Exchange Resins; D004337:Drug Carriers; D005229:Fatty Acids, Monounsaturated; D008055:Lipids; C086724:Lipofectamine; D008081:Liposomes; D000644:Quaternary Ammonium Compounds; C087276:(3-dimyristyloxypropyl)(dimethyl)(hydroxyethyl)ammonium; D004247:DNA; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane