Immunohistochemical localization of bone morphogenetic proteins and the receptors in epiphyseal growth plate.
The expression of bone morphogenetic proteins (BMPs) and BMP receptors (BMPRs) in the epiphyseal growth plate has not been clarified. In this study, we studied immunohistochemically the spatial and temporal localization of BMP-2/4, osteogenic protein-1 (OP-1, or BMP-7), and BMP receptors (BMPR-IA, BMPR-IB, and BMPR-II) in the epiphyseal plate of growing rats. The proximal parts of tibia in growing rats were observed. At 12 weeks after birth, BMP-2/4 and OP-1 were expressed markedly in proliferating and maturing chondrocytes. BMPR-IA, IB and II were clearly co-expressed in proliferating and maturing chondrocytes, and the expression was decreased in hypertrophic chondrocytes. At 24 weeks, the expression of BMP-2/4 and OP-1 was decreased, but BMPRs were still well-expressed in proliferating chondrocytes. The temporal and spatial expression of BMP and BMPR suggests that BMP and BMP receptors play roles in the multistep cascade of enchondral ossification in the epiphyseal growth plate.
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Japan. shunji@med1.kufm.kagoshima-u.ac.jp
Y
Y
Yazaki
S
S
Matsunaga
T
T
Onishi
T
T
Nagamine
N
N
Origuchi
T
T
Yamamoto
Y
Y
Ishidou
T
T
Imamura
T
T
Sakou
D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D052004:Bone Morphogenetic Protein Receptors; D019485:Bone Morphogenetic Proteins / Q000032:analysis / Q000096:biosynthesis*; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D002455:Cell Division; D006132:Growth Plate / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D010012:Osteogenesis; D051381:Rats; D017208:Rats, Wistar; D011956:Receptors, Cell Surface / Q000032:analysis / Q000096:biosynthesis*; D017978:Receptors, Growth Factor
D019485:Bone Morphogenetic Proteins; D011956:Receptors, Cell Surface; D017978:Receptors, Growth Factor; D052004:Bone Morphogenetic Protein Receptors