A radioimmunoassay for the determination of angiotensin II in elasmobranch fish.
A homologous radioimmunoassay was developed to determine the concentration of angiotensin II (Asn1, Pro3, Ile5)-Ang II) in elasmobranchs. Cross-reactivity with elasmobranch angiotensin I and other heterologous angiotensins was high and therefore all potentially cross-reacting angiotensins were separated by high performance liquid chromatography after prior extraction with Sep-Pak C18 cartridges. The validity of the assay for the determination of elasmobranch Ang II was demonstrated by parallelism with a series of Ang II standards with serially diluted elasmobranch plasma extracts. Overall recovery of elasmobranch Ang II added to a plasma pool was 75.1 +/- 5.2%. Plasma Ang II concentrations measured by our RIA were similar in fish adapted to 70, 100, or 120% SW at 139 +/- 20.1, 109 +/- 15.3, and 119 +/- 16.3 fmol . ml-1, respectively.
School of Environmental and Evolutionary Biology, University of St. Andrews, Fife, KY16 8LB, United Kingdom.
M L
ML
Tierney
Y
Y
Takei
N
N
Hazon
D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000804:Angiotensin II / Q000097:blood*; D000818:Animals; D003429:Cross Reactions; D004284:Dogfish / Q000097:blood*; D005260:Female; D016015:Logistic Models; D008297:Male; D011863:Radioimmunoassay; D015203:Reproducibility of Results
D000804:Angiotensin II