Identification of functional domains in Efb, a fibrinogen binding protein of Staphylococcus aureus.
Staphylococcus aureus produces and secretes a protein, Efb, that binds to fibrinogen, seems to be required for virulence, and may benefit the microorganism by delaying wound healing. Interactions of Efb with fibrinogen are influenced by divalent metal cations, including Ca2+. Increasing concentrations of Ca2+ increased the binding of fibrinogen to immobilized Efb, whereas binding of Efb to immobilized fibrinogen was decreased with increasing Ca2+ concentration. Studies with synthetic peptides showed that peptides from the carboxyl terminal half of Efb bound to soluble fibrinogen and enhanced the binding of fibrinogen to Efb. A peptide corresponding to a repeated sequence in the amino terminal half of the protein also bound fibrinogen and inhibited binding of fibrinogen to Efb. These results may provide clues to the biological function of Efb and aid in the rational design of agents to block the Efb fibrinogen interaction.
Department of Immunology, Microbiology, Pathology, and Infectious Diseases, Huddinge Hospital, Karolinska Institute, Sweden. david.wade@impi.ki.se
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Wade
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Palma
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Löfving-Arvholm
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Sällberg
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Silberring
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Flock
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D002122:Calcium Chloride / Q000494:pharmacology; D002352:Carrier Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D007700:Kinetics; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D018629:Spectrometry, Mass, Secondary Ion; D013211:Staphylococcus aureus / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence
D001426:Bacterial Proteins; D002352:Carrier Proteins; C114014:Efb protein, Staphylococcus aureus; D010446:Peptide Fragments; D005340:Fibrinogen; D002122:Calcium Chloride; D002118:Calcium