[Functional analysis of nuclear hormone receptors].
Nuclear hormone receptors are transcription factors which regulate gene transcription by lipophilic hormones. The functional domains such as transactivation, DNA binding, ligand binding and dimerization are mapped in the distinct region of the receptors. To analyze these functions, ligand binding assay, gel shift assay, transfection study, GST pull-down method, immunoprecipitation method, two hybrid system and yeast functional assay are employed. These assays have been shown to clarify the molecular basis of the disorders which are due to mutation in the receptors.
Department of Endocrinology and Metabolism, Nagoya University.
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Seo
D000483:Alleles; D004247:DNA / Q000378:metabolism; D019281:Dimerization; D005822:Genetic Vectors; D006728:Hormones / Q000235:genetics; D006801:Humans; D009154:Mutation; D011233:Precipitin Tests; D011987:Receptors, Steroid / Q000235:genetics / Q000502:physiology*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014157:Transcription Factors; D015533:Transcriptional Activation; D014162:Transfection
D006728:Hormones; D011987:Receptors, Steroid; D014157:Transcription Factors; D004247:DNA