Glutamine deprivation induces apoptosis in intestinal epithelial cells.
BACKGROUND
Glutamine is the most abundant amino acid in the blood, and its deprivation leads to gut mucosal atrophy. The small intestinal mucosa is maintained by a balance between cell proliferation and cell death by apoptosis. We reported that glutamine is required for nitrogen-stimulated proliferation in intestinal epithelial cells. We do not know whether glutamine regulates apoptosis in the gut. The purpose of this study is to determine whether glutamine deprivation induces apoptosis in rat intestinal epithelial (RIE-1) cells and to compare the effect of glutamine starvation with that of methionine and cysteine (Met/Cys) starvation.


METHODS
RIE-1 cells were deprived of either glutamine or Met/Cys for 24 hours. Cell numbers were determined by cell counting and tetrazolium enzymatic assay. Apoptosis was quantified by Annexin V assay and confirmed by DNA gel electrophoresis and Hoecsht nuclear staining.


RESULTS
Deprivation of glutamine or Met/Cys resulted in decreased cell numbers. However, only the glutamine-deprived group showed significant induction of apoptosis with increased Annexin V staining, DNA laddering, and nuclear condensation.


CONCLUSIONS
This study provides biochemical and morphologic evidence that glutamine deprivation induces apoptosis in rat intestinal epithelial cells. In contrast, Met/Cys starvation suppresses cell number without induction of apoptosis. These results suggest that glutamine serves as a specific survival factor in enterocytes.
Department of Surgery, University of Texas Medical Branch, Galveston 77555-0542, USA.
H T
HT
Papaconstantinou
K O
KO
Hwang
S
S
Rajaraman
M R
MR
Hellmich
C M
CM
Townsend
T C
TC
Ko
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D003545:Cysteine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism / Q000494:pharmacology*; D007422:Intestines / Q000166:cytology*; D008715:Methionine / Q000494:pharmacology; D051381:Rats
D005973:Glutamine; D008715:Methionine; D003545:Cysteine