Antigenic phenotype of early intra-embryonic lymphoid progenitors in the chicken.
The stem cells for the definitive haematopoiesis are derived from intra-embryonic sources originally described in an avian model and later also in mammals. However, the molecular make-up of the early embryonic haematopoietic progenitors is not yet clearly defined. We have recently characterized the phenotype of prethymic intra-embryonic progenitors capable of thymic colonization. Here we studied the ontogeny of cell-surface antigens HEMCAM, alpha2beta1 integrin, thrombomucin, chL12 and c-kit and their co-expression on prethymic T-cell progenitors. The early intra-embryonic expression of avian B-cell antigen chB6 was also demonstrated on cells derived from the intra-embryonic areas. We suggest that in the chicken, embryonic B-cell progenitors segregate earlier than T-cell progenitors in the differentiation of multipotent haematopoietic stem cells to committed progenitors.
Lampisuo
M
M
Turku Immunology Centre and Department of Medical Microbiology, University of Turku, Finland.
Katevuo
K
K
Lassila
O
O
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D015415:Biomarkers; D002642:Chick Embryo; D002645:Chickens; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D016130:Immunophenotyping; D019891:Leukopoiesis; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010641:Phenotype; D013950:Thymus Gland / Q000276:immunology
D000954:Antigens, Surface; D015415:Biomarkers