Expression of somatotropin receptor messenger ribonucleic acid in bovine tissues.
The somatotropin receptor mRNA is controlled by at least two different gene promoters that generate two variants with different exon 1 sequences (1A and 1B). The location of 1A and 1B somatotropin receptor mRNA within cattle tissues and, hence, the tissue specificity of the 1A and 1B promoters are unknown. In addition, the cDNA sequence of the 1B somatotropin receptor has not been determined. Our objective, therefore, was to sequence a cDNA for the 1B somatotropin receptor and to analyze bovine tissues for expression of 1A and 1B somatotropin receptor mRNA. Twenty adult tissues and six fetal tissues were collected at slaughter from each of four cows and two fetuses. Messenger RNA was analyzed using ribonuclease protection assays. The adult liver expressed both 1A and 1B mRNA. All other adult tissues expressed 1B mRNA but not 1A mRNA. The greatest amount of 1B mRNA was detected in liver and adipose (abdominal and subcutaneous) tissues. Other tissues had approximately one-half to one-tenth of the amount of 1B mRNA in the liver or adipose tissue. Fetal tissues (including fetal liver) expressed 1B mRNA and not 1A mRNA. Based on cDNA sequencing, the protein encoded by the 1A and 1B mRNA was nearly identical. We concluded that 1A somatotropin receptor mRNA is specific to adult bovine liver. Other adult and fetal bovine tissues expressed 1B somatotropin receptor mRNA with a predicted protein sequence that was similar to the 1A somatotropin receptor.
Lucy
M C
MC
Department of Animal Sciences, University of Missouri, Columbia 65211, USA.
Boyd
C K
CK
Koenigsfeld
A T
AT
Okamura
C S
CS
D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002417:Cattle / Q000378:metabolism*; D018076:DNA, Complementary / Q000737:chemistry; D005091:Exons; D005260:Female; D005333:Fetus / Q000737:chemistry; D015870:Gene Expression; D008099:Liver / Q000737:chemistry; D009928:Organ Specificity; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis*; D011986:Receptors, Somatotropin / Q000235:genetics*; D012260:Ribonucleases; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D012333:RNA, Messenger; D011986:Receptors, Somatotropin; D012260:Ribonucleases