Simultaneous detection of high energy phosphates and metabolites of glycolysis and the Krebs cycle by HPLC.
For the detailed analysis of energy metabolism, a HPLC method is described allowing the single-run separation and quantification of most metabolites from glycolysis and the Krebs cycle including the high energy phosphates. With a detection limit in the picomolar range this method is even applicable when only small sample sizes of tissue are obtained.
Department of Cardiology, University of Heidelberg, Germany. Achim_Vogt@ukl.uni-heidelberg.de
A M
AM
Vogt
C
C
Ackermann
T
T
Noe
D
D
Jensen
W
W
Kübler
D000227:Adenine Nucleotides / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002952:Citric Acid Cycle; D004734:Energy Metabolism; D006019:Glycolysis; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D017202:Myocardial Ischemia / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013403:Sugar Phosphates / Q000032:analysis / Q000378:metabolism*
D000227:Adenine Nucleotides; D013403:Sugar Phosphates