Molecular procedure for rapid detection of Burkholderia mallei and Burkholderia pseudomallei.
A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel.
Max von Pettenkofer Institut, D-80336 Munich, Germany. Adolf.Bauernfeind@mvp-bak.med.uni-muenchen.de
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Bauernfeind
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Roller
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Meyer
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Jungwirth
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Schneider
D001483:Base Sequence; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019121:Burkholderia Infections / Q000175:diagnosis* / Q000517:prevention & control / Q000635:transmission; D016957:Burkholderia pseudomallei / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D006801:Humans; D017056:Medical Laboratory Personnel; D008554:Melioidosis / Q000175:diagnosis* / Q000517:prevention & control / Q000635:transmission; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009784:Occupational Diseases / Q000382:microbiology / Q000517:prevention & control; D016133:Polymerase Chain Reaction / Q000379:methods*; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D004275:DNA, Ribosomal; D012338:RNA, Ribosomal, 23S