In vivo gene transfer to the mouse oviduct epithelium.
OBJECTIVE
To transfect the mouse oviduct in vivo.


METHODS
Prospective study.


METHODS
Research laboratory.


METHODS
Sixteen female Swiss albino mice.


METHODS
The left oviduct of 10 female mice was instilled with a liposome DNA solution. In addition, 2 control mice received liposome solution, 2 received phosphate-buffered saline solution, and 2 received no injection.


METHODS
The expression of the gene transfected (beta-galactosidase) was detected in the oviduct epithelium with the use of a routine histochemical analysis.


RESULTS
The 90% of the female mice that were transfected with liposome/beta-Gal complexes expressed the gene in the oviduct mucosa. The controls did not show beta-Gal expression.


CONCLUSIONS
Cationic liposome/DNA complexes can be used for in vivo transfection of the mouse fallopian tubes. The foreign gene expression occurs in clusters of cells located along the mucosa of the isthmus and juncture regions.
Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
M
M
Relloso
P
P
Esponda
D000818:Animals; D004848:Epithelium / Q000378:metabolism; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D018014:Gene Transfer Techniques; D008081:Liposomes; D051379:Mice; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics*
D008081:Liposomes; D001616:beta-Galactosidase