Lymphostin (LK6-A), a novel immunosuppressant from Streptomyces sp. KY11783: taxonomy of the producing organism, fermentation, isolation and biological activities.
In the course of screening for inhibitors of the lymphocyte kinase, Lck (p56lck), aiming at novel immunosuppressants, we isolated a novel alkaloid, lymphostin (LK6-A), from the culture broth of Streptomyces sp. KY11783. Lymphostin was produced in a fermentation medium supplemented with a highly porous polymer resin, which prevented the degradation of this compound in the culture broth. Lymphostin inhibited the kinase activity of Lck with an IC50 value of 0.05 microM, and exhibited potent inhibitory activity against the mixed lymphocyte reaction (MLR) with an IC50 value of 0.009 microM.
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.
H
H
Nagata
K
K
Ochiai
Y
Y
Aotani
K
K
Ando
M
M
Yoshida
I
I
Takahashi
T
T
Tamaoki
D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D006801:Humans; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000037:antagonists & inhibitors*; D008826:Microbial Sensitivity Tests; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000378:metabolism*
D007166:Immunosuppressive Agents; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)