Early function of HIV-1 Gag proteins is cell-dependent.
Various gag gene mutants of human immunodeficiency virus type 1 (HIV-1) were monitored for their replication potentials and defective replication sites in various CD4-positive T-cell lines. Some matrix, capsid, and nucleocapsid mutants displayed a replication defect in a cell-dependent manner. The single-round replication assays demonstrated that these mutants were defective at an early infection phase also in a cell-dependent way. These results indicated that interaction of a cell factor(s) and Gag proteins is involved in an early process of HIV-1 replication.
Department of Virology, University of Tokushima School of Medicine, Japan.
M
M
Kawamura
R
R
Shimano
R
R
Inubushi
H
H
Akari
A
A
Adachi
D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis; D015683:Gene Products, gag / Q000378:metabolism*; D015750:Genes, gag; D054303:HIV Reverse Transcriptase / Q000096:biosynthesis; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014162:Transfection; D014779:Virus Replication
D015683:Gene Products, gag; D011994:Recombinant Proteins; D015500:Chloramphenicol O-Acetyltransferase; D054303:HIV Reverse Transcriptase