Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis.
Two short tandem repeat (STR) systems, HumTPO and HumLPL, were investigated in 218 unrelated Japanese living in Gunma and Fukui Prefectures by means of discontinuous, horizontal polyacrylamide gel electrophoresis and silver staining. Five and six alleles were identified at the HumTPO and HumLPL loci, respectively. No deviations from the Hardy-Weinberg equilibrium or differences in genotype frequency were found between the two Japanese populations in the two systems. Our detection methods offered several advantages including time-saving and safe electrophoretic procedures, and fast and easy silver-staining, in comparison with standard and conventional STR analysis using vertical polyacrylamide gel electrophoresis.
Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
H
H
Takeshita
T
T
Yasuda
T
T
Nakajima
O
O
Hosomi
Y
Y
Nakashima
S
S
Tsutsumi
K
K
Kishi
D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007564:Japan; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*