Microtubule disruption potentiates phenylephrine-induced vasoconstriction in rat mesenteric arterial bed.
The pressor response induced by phenylephrine in the rat isolated mesenteric arterial bed was significantly increased following treatment with nocodazole, a drug that disassembles microtubules (10 microM, 90 min). This increase was even greater in the presence of nitric oxide (NO) synthase inhibition, and completely reversed by paclitaxel (20 microM), a stabilizer of microtubules. These results demonstrate that disassembly of microtubules enhances vasoconstriction to receptor activation, suggesting that the microtubules modulate the transduction of intracellular signals in endothelium and vascular smooth muscle cells.
Leite
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Department of Physiology, University of Michigan Medical School, Ann Arbor 48109-0622, USA. rlicbfar@mono.icb.ufmg.br
Webb
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D000818:Animals; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology; D008638:Mesenteric Arteries / Q000187:drug effects* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008870:Microtubules / Q000187:drug effects* / Q000502:physiology; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000502:physiology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D015739:Nocodazole / Q000633:toxicity*; D017239:Paclitaxel / Q000494:pharmacology; D010656:Phenylephrine / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects; D013329:Structure-Activity Relationship; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000633:toxicity*
D014662:Vasoconstrictor Agents; D010656:Phenylephrine; D019001:Nitric Oxide Synthase; D017239:Paclitaxel; D015739:Nocodazole