Use of principal component analysis for the evaluation of the retention behaviour of monoamine oxidase inhibitory drugs on beta-cyclodextrin column.
The retention of 17 monoamine oxidase inhibitory drugs (proparlgylamine derivatives) were determined on a beta-cyclodextrin polymer (beta CDP)-coated silica column using ethanol-0.05 M K2HPO4 (6:4 v/v) as the eluent. The relative strength of interaction between the drugs and a water soluble beta-cyclodextrin polymer was determined by charge-transfer chromatography carried out on reversed-phase TLC layers. The relationship between capacity factors, physicochemical parameters and inclusion complex forming capacity of the monoamine oxidase inhibitory drugs were evaluated by stepwise regression analysis and by principal component analysis (PCA) followed by two-dimensional nonlinear mapping and varimax rotation. Calculations indicated that the retention of monoamine oxidase inhibitory drugs on beta CDP column is mainly governed by their steric and lipophylic parameters. Significant linear correlations were found between the corresponding coordinates of varimax rotation and two-dimensional nonlinear maps proving the suitability of both methods for the reduction of dimensionality of complicated data matrices.
Forgács
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Central Research Institute for Chemistry, Hungarian Academy of Sciences, Budapest.
D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D003505:Cyclodextrins / Q000737:chemistry*; D005503:Food Additives / Q000737:chemistry*; D008996:Monoamine Oxidase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D015999:Multivariate Analysis; D010293:Pargyline / Q000031:analogs & derivatives; D011437:Propylamines; D012995:Solubility; D047392:beta-Cyclodextrins
D003505:Cyclodextrins; D005503:Food Additives; D008996:Monoamine Oxidase Inhibitors; D011437:Propylamines; D047392:beta-Cyclodextrins; C009054:propargylamine; D010293:Pargyline; C031215:betadex