Human parathyroid hormone does not influence human erythropoiesis in vitro.
BACKGROUND
Although renal anaemia is associated with secondary hyperparathyroidism, the relationship of both conditions remains obscure. Previously it was reported that high levels of bovine parathyroid hormone (PTH) did not inhibit in vitro human erythropoiesis, but whether human PTH inhibits in vitro human erythropoiesis has not been determined.


METHODS
To clarify the direct effects of human biologically active N-terminal (1-34) PTH and intact (1-84) PTH on human haematopoietic progenitor growth, we investigated colony assays of human erythropoiesis and granulomonopoiesis.


RESULTS
Neither N-terminal PTH (300 ng/ml) nor intact PTH (5000 pg/ml) inhibited haematopoietic progenitor growth.


CONCLUSIONS
Our findings confirm that human PTH does not directly inhibit human erythropoiesis.
Third Department of Internal Medicine, Akita University School of Medicine, Japan.
A
A
Komatsuda
M
M
Hirokawa
T
T
Haseyama
T
T
Horiuchi
H
H
Wakui
H
H
Imai
A B
AB
Miura
D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000187:drug effects; D004920:Erythropoiesis / Q000187:drug effects*; D006098:Granulocytes / Q000187:drug effects; D006801:Humans; D018374:LLC-PK1 Cells; D008264:Macrophages / Q000187:drug effects; D010281:Parathyroid Hormone / Q000494:pharmacology*; D013234:Stem Cells / Q000187:drug effects; D013552:Swine; D019379:Teriparatide / Q000494:pharmacology
D010281:Parathyroid Hormone; D019379:Teriparatide