Two-dimensional gel analysis of midgut proteins of Anopheles stephensi lines with different susceptibility to Plasmodium falciparum infection.
Little is known about the composition of the mosquito midgut which plays a central role in the development and subsequent transmission of malaria parasites. As a first step towards the characterization of mosquito midgut molecules involved in the transmission of malaria parasites, we analysed two-dimensional gel electrophoresis patterns of the midgut proteins of sugar-fed and blood-fed Anopheles stephensi lines of different susceptibility to P. falciparum infection. Two lines fully susceptible and one line (Pb3-9A) of reduced susceptibility were used. In the refractory line ookinetes do develop but are only inefficiently transformed into oocysts (Feldmann & Ponnudurai, 1989). The protein profiles of midguts from all sugar-fed mosquito lines were similar. However, after blood feeding, the midgut of the fully susceptible lines contained proteins not found in the midgut of line Pb3-9A. Twenty-nine such proteins were detected and are candidates for involvement in the interaction between the mosquito midgut and P. falciparum.
Prévot
G I
GI
Unité d'Ecologie des Systèmes Vectoriels, Institut Pasteur, France.
Laurent-Winter
C
C
Feldmann
A M
AM
Rodhain
F
F
Bourgouin
C
C
D000818:Animals; D000852:Anopheles / Q000737:chemistry* / Q000469:parasitology*; D002241:Carbohydrates; D004064:Digestive System; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005260:Female; D006801:Humans; D019476:Insect Proteins / Q000032:analysis*; D010963:Plasmodium falciparum / Q000502:physiology*
D002241:Carbohydrates; D019476:Insect Proteins