Evidence for Jak-STAT interaction in channel catfish lymphoid cells.
Herein is described the identification of a putative Jak molecule in the channel catfish. A commercially available polyclonal antibody against human Jak1 was shown to react in Western blot analyses with molecules of approximately 140, 80, and 60 kDa from cell lysates of catfish long-term lymphoid cell lines. It is postulated that the 140 kDa protein is a catfish Jak homolog and the 80 and 60 kDa proteins may represent breakdown products of the 140 kDa protein. Both the 140 and 60 kDa proteins appear to be constitutively tyrosine phosphorylated in channel catfish long-term leukocyte lines. Furthermore, immuno-precipitation studies indicate an association between the Jak molecule and a STAT molecule in cloned T cell lines, and to a much lesser extent in cloned B cell lines, indicative of a possible autocrine stimulatory pathway in catfish long-term T cell lines.
Rycyzyn
M A
MA
Department of Microbiology, University of Mississippi Medical Center, Jackson 39216, USA.
Wilson
M R
MR
Clem
L W
LW
Miller
N W
NW
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007059:Ictaluridae / Q000378:metabolism*; D053613:Janus Kinase 1; D053616:Janus Kinase 3; D008214:Lymphocytes / Q000378:metabolism*; D008970:Molecular Weight; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*
D004268:DNA-Binding Proteins; D015534:Trans-Activators; D011505:Protein-Tyrosine Kinases; C507904:JAK1 protein, human; C507933:JAK3 protein, human; D053613:Janus Kinase 1; D053616:Janus Kinase 3