Kappa-casein polymorphisms in Indian dairy cattle and buffalo: a new genetic variant in buffalo.
We screened 57 Sahiwal cattle (Bos indicus) and 53 Murrah, 19 Nili-Ravi and 11 Egyptian buffaloes (Bubalus bubalis) to detect the polymorphisms at kappa-casein (CSN3) gene using the polymerase chain reaction (PCR). CSN3 A and B alleles were identified by PCR-RFLP using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (Taql) and at codon 148 (HindIII or HinfI) in cattle. The frequency of CSN3 B allele in the Sahiwal cattle was estimated as 0.16 with no homozygous BB animal. Using the same set of primers as used in the Sahiwal cattle, a part of exon IV of buffalo CSN3 gene was amplified, but restriction enzyme analysis using HindIII/HinfI and TaqI did not reveal any polymorphism. However, DNA sequencing of the amplified fragment (GenBank Acc. No. U96662) revealed one polymorphism at codon 135 (ThrACC -->I1eATC) in buffalo; the frequencies of 135 Thr/Ile alleles were estimated as 0.88 and 0.12, respectively.
Mitra
A
A
Dairy Cattle Breeding Division, National Dairy Research Institute, Karnal, India.
Schlee
P
P
Krause
I
I
Blusch
J
J
Werner
T
T
Balakrishnan
C R
CR
Pirchner
F
F
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002364:Caseins / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D008892:Milk / Q000737:chemistry; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
D002364:Caseins