[Kinetics of the changes in free calcium ions concentration in human spermatozoa under the effect of progesterone].
In the studies, changes in concentration of free calcium ions were analysed in human spermatozoa, as affected by steroid hormones. The experiments were performed on human sperm preparations originating from healthy donors which fulfilled criteria of WHO (1992). The cells separated by the swim-up technique were loaded with the Fura-2AM (4 mM). The changes in concentration of Ca2+ were monitored employing the ratio-imaging technique (MagiCal, Applied Imaging, England). We examined the influence of progesterone, hydrocortisone, RU 486, testosterone, 17 b-estradiol, pro-BSA on Ca2+ concentration. The performed experiments demonstrated that already a few seconds (5-150s) exposure to progesterone and prog-BSA induced a dose dependent increase in Ca2+ concentration in normal spermatozoa. The cell characteristics (no transcription), reaction time, dose dependent and the reaction to pro-BSA, show that progesterone acts through the membrane receptors.
Zakładu Radiobiologii i Biologii Komórki Akademii Medycznej im. Karola Marcinkowskiego w Poznaniu.
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M
Kotwicka
J B
JB
Warchoł
D015220:Calcium Channels / Q000032:analysis* / Q000378:metabolism*; D004958:Estradiol / Q000493:pharmacokinetics; D006801:Humans; D006854:Hydrocortisone / Q000493:pharmacokinetics; D008297:Male; D015735:Mifepristone / Q000493:pharmacokinetics; D011374:Progesterone / Q000493:pharmacokinetics*; D013094:Spermatozoa / Q000737:chemistry* / Q000187:drug effects*; D013256:Steroids / Q000493:pharmacokinetics; D013739:Testosterone / Q000493:pharmacokinetics
D015220:Calcium Channels; D013256:Steroids; D015735:Mifepristone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D006854:Hydrocortisone