Cardiac protein kinase C expression in two models of cardiac hypertrophy associated with an activated cardiac renin-angiotensin system: effects of experimental hyperthyroidism and genetic hypertension (the mRen-2 rat).
There is evidence for a role of protein kinase C (PKC) in the development of cardiac hypertrophy. We examined the expression of individual PKC isoforms in the adult rat heart in two distinct, well-characterised in vivo models of cardiac hypertrophy associated with an activated cardiac renin-angiotensin system, namely experimental hyperthyroidism and the TGR(mRen2)27 rat. The cardiac expression of a range of PKC isoforms (PKC-alpha, PKC-omega, PKC-epsilon, PKC-gamma, and PKC-tau) was examined by immuno-blotting. Our work demonstrates that the expression of total cardiac nPKC-omega and nPKC-epsilon relative to protein is selectively and differentially modified in these models. A consistent up-regulation of nPKC-omega in conjunction with overall down-regulation of nPKC-epsilon was observed in both models. The expression of other PKC isoforms was unaffected. The divergent responses of the expression of the two nPKC isoforms to an activated cardiac renin-angiotensin system in vivo in adulthood suggest that these individual nPKC isoforms subserve specific roles in the response.
Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, London, UK.
L G
LG
Fryer
M J
MJ
Holness
J B
JB
Decock
M C
MC
Sugden
D000818:Animals; D030801:Animals, Genetically Modified; D005260:Female; D006973:Hypertension / Q000235:genetics / Q000378:metabolism; D006980:Hyperthyroidism / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000201:enzymology*; D015151:Immunoblotting; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008955:Models, Cardiovascular; D009206:Myocardium / Q000201:enzymology*; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012083:Renin / Q000235:genetics; D012084:Renin-Angiotensin System / Q000502:physiology*
D007527:Isoenzymes; D011493:Protein Kinase C; D012083:Renin