Two-dimensional gel analysis of proteins in the Drosophila wing imaginal disc mutants fat and lethal (2) giant discs.
High-resolution two-dimensional (2D) gel electrophoresis coupled with computer analysis has been used to construct a quantitative protein database of Drosophila mature wing imaginal discs. The level of expression for all of the detected proteins has been quantitatively determined. This database has been used to evaluate changes in the patterns of protein synthesis in wing imaginal discs from two Drosophila melanogaster mutants with abnormal wing disc development: fat (ft) and two different alleles of lethal (2) giant disc (l(2)gd). Patterns of pulse-labeled proteins of the different mutants show variations in both qualitative and quantitative parameters of synthesis. In this comparison we have detected specific sets of protein changes characteristic of both alleles of the same locus and a set of protein changes common to both loci. How the abnormal expression of these proteins relates to the abnormal process of mutant hyperplasia is discussed.
Centro de Biología Molecular "Severo Ochoa," CSIC-UAM, Universidad Autónoma de Madrid, Madrid, Cantoblanco, 28049-, Spain. jfsantaren@trasto.cbm.uam.es
J F
JF
Santarén
M
M
Milán
A
A
García-Bellido
D000818:Animals; D015820:Cadherins; D019992:Databases as Topic; D004330:Drosophila / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D016147:Genes, Tumor Suppressor / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D025521:Tumor Suppressor Proteins
D015820:Cadherins; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D025521:Tumor Suppressor Proteins; C081435:l(2)gl protein, Drosophila