Conserved gene structure and genomic linkage for D-dopachrome tautomerase (DDT) and MIF.
Macrophage migration inhibitory factor (MIF) and D-dopachrome tautomerase (DDT) are small proteins, which are related both by sequence and by in vitro enzyme activity. Here we show that the gene for DDT in human and mouse is identical in exon structure to MIF. Both genes have two introns that are located at equivalent positions, relative to a twofold repeat in protein structure. Although in similar positions, the introns are in different phases relative to the open reading frame. Other members of this superfamily exist in nematodes and a plant, and a related gene in C. elegans shares an intron position with MIF and DDT. In addition to similarities in structure, the genes for DDT and MIF are closely linked on human Chromosome (Chr) 22 and mouse Chr 10.
Section on Molecular Structure and Function, National Eye Institute, Building 6 Room 331, National Institutes of Health, Bethesda, Maryland 20892-2740, USA.
N
N
Esumi
M
M
Budarf
L
L
Ciccarelli
B
B
Sellinger
C A
CA
Kozak
G
G
Wistow
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D002460:Cell Line; D002874:Chromosome Mapping; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D003001:Cloning, Molecular; D005091:Exons; D005347:Fibroblasts; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D008040:Genetic Linkage; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D019746:Intramolecular Oxidoreductases / Q000235:genetics*; D007438:Introns; D008263:Macrophage Migration-Inhibitory Factors / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D009928:Organ Specificity / Q000235:genetics; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D008263:Macrophage Migration-Inhibitory Factors; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase