Influence of temperature upon protein partitioning in poly(ethylene glycol)-salt aqueous two-phase systems close to the critical point with some observations relevant to the partitioning of particles.
The partition of proteins in a poly(ethylene glycol) (PEG)-potassium phosphate aqueous two-phase system has been studied in systems which were produced by manipulation of the equilibrium temperature of a monophasic mixture composition close to the critical point. Precise control of the composition enabled the formation of biphasic systems lying very close to the critical point. A high-performance liquid chromatographic method for analysis of the phase compositions is presented. The generally applicability of the method to the partition of proteins and other particles is discussed.
Biochemical Recovery Group, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
S
S
de Belval
B
B
le Breton
J
J
Huddleston
A
A
Lyddiatt
D002851:Chromatography, High Pressure Liquid; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols; D017680:Potassium Compounds / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012492:Salts; D013050:Spectrometry, Fluorescence; D013696:Temperature
D010710:Phosphates; D017680:Potassium Compounds; D011506:Proteins; D012492:Salts; D011092:Polyethylene Glycols; C013216:potassium phosphate