Specificity of isoelectric focusing-purified antigens in the diagnosis of human cysticercosis.
Specific antigens were isolated from the cystic fluid of larval Taenia solium by preparative isoelectric focusing (PIEF). A total of 20 fractions were produced by a rotating ampholine column with pI 3-10 ampholytes. The specificity of each fraction (F) was tested by double-antibody enzyme-linked immunosorbent assay (ELISA) using antisera from patients suffering from cysticercosis or one of six other parasitic diseases. F8-F15 cross-reacted strongly with sera from patients with hydatidosis. F9 and F10 also cross-reacted with the antisera against ascariasis and F15, with antisera against angiostrongylosis. However, F16 and F17 were highly specific as they yielded no cross-reaction with any of the heterologous antisera. PIEF is a good method for the production of specific antigens from larval T. solium because it is easy to perform and relatively inexpensive to run.
Department of Zoology, The University of Hong Kong, Hong Kong.
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D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification*; D003429:Cross Reactions; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007525:Isoelectric Focusing; D007526:Isoelectric Point; D012680:Sensitivity and Specificity; D013621:Taenia / Q000276:immunology
D000947:Antigens, Helminth