Sequencing of cDNA clones from the genetic map of tomato (Lycopersicon esculentum).
The dense RFLP linkage map of tomato (Lycopersicon esculentum) contains >300 anonymous cDNA clones. Of those clones, 272 were partially or completely sequenced. The sequences were compared at the DNA and protein level to known genes in databases. For 57% of the clones, a significant match to previously described genes was found. The information will permit the conversion of those markers to STS markers and allow their use in PCR-based mapping experiments. Furthermore, it will facilitate the comparative mapping of genes across distantly related plant species by direct comparison of DNA sequences and map positions. [cDNA sequence data reported in this paper have been submitted to the EMBL database under accession nos. AA824695-AA825005 and the dbEST_Id database under accession nos. 1546519-1546862.]
Institute for Plant Genetics and Crop Plant Research, D-06466 Gatersleben, Germany. ganal@ipk-gatersleben.de
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Ganal
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Czihal
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Hannappel
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Kloos
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D017360:Arabidopsis / Q000235:genetics; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary; D018744:DNA, Plant / Q000235:genetics*; D016208:Databases, Factual; D017343:Genes, Plant; D005819:Genetic Markers; D018551:Solanum lycopersicum / Q000235:genetics*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D016324:Sequence Tagged Sites; D014158:Transcription, Genetic
D018076:DNA, Complementary; D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins