Identification of in vivo phosphorylation sites for basic-directed kinases in murine mdr1b P-glycoprotein by combination of mass spectrometry and site-directed mutagenesis.
Albert Einstein College of Medicine, Bronx, New York 10461, USA.
J S
JS
Glavy
M
M
Wolfson
E
E
Nieves
E K
EK
Han
C P
CP
Yang
S B
SB
Horwitz
G A
GA
Orr
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003001:Cloning, Molecular / Q000379:methods; D003488:Cyanogen Bromide; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D018432:Drug Resistance, Multiple; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D008264:Macrophages; D051379:Mice; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010449:Peptide Mapping / Q000379:methods; D010748:Phosphopeptides / Q000737:chemistry / Q000302:isolation & purification; D010766:Phosphorylation; D015202:Protein Engineering / Q000379:methods; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D018629:Spectrometry, Mass, Secondary Ion / Q000379:methods; D014747:Vinblastine / Q000493:pharmacokinetics / Q000633:toxicity*
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D010446:Peptide Fragments; D010748:Phosphopeptides; D011994:Recombinant Proteins; D014747:Vinblastine; D017868:Cyclic AMP-Dependent Protein Kinases; D011493:Protein Kinase C; D003488:Cyanogen Bromide