Anti-immunoglobulin E antibody treatment blocks histamine release and tissue contraction in sensitized mice.
Inhibition of antigen-specific IgE response has been shown to lead to amelioration of allergic disease symptoms. In an effort to design a therapy aimed at decreasing IgE levels, we reported previously that treatment of mice with an anti-IgE antibody coincident with the primary antigen immunization resulted in significant decreases in antigen-specific IgE synthesis, without substantially altering IgG levels. In the present study, we employed this mouse model and a surrogate antibody to investigate the capacity of anti-IgE treatment to block an established IgE response in vivo. Results of these experiments suggest that anti-IgE treatment concomitant with an antigen boost results in removal of detectable circulating IgE for at least 7 weeks (the duration of the study). Moreover, tissues removed from mice following anti-IgE treatment failed to release histamine and contract in response to antigen challenge ex vivo. These findings demonstrate that reduction of circulating IgE correlates to an inhibition of tissue mast cell sensitization and mediator release in response to antigen challenge and further supports the concept of anti-IgE treatment as a promising therapy for the treatment of allergic disease.
School of Veterinary Medicine, University of Wisconsin, Madison, USA.
M
M
Haak-Frendscho
R
R
Saban
R L
RL
Shields
P M
PM
Jardieu
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D004140:Dinitrophenols / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology; D006636:Histamine Release / Q000276:immunology*; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D010047:Ovalbumin / Q000276:immunology; D014132:Trachea / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D004140:Dinitrophenols; D006241:Haptens; C045521:dinitrophenol-ovalbumin; D007073:Immunoglobulin E; D010047:Ovalbumin