Sequencing and phylogenetic analysis of the protease gene, and genetic mapping of bovine adenovirus type 10 define its relatedness to other bovine adenoviruses.
The complete genome of a bovine adenovirus (BAV) type 10 isolate was molecularly cloned and partially sequenced. The encoded proteins were predicted by computer analysis of the DNA sequences of the ends or the entire length of the cloned viral fragments, and thus a rough genetic map was constructed. The protease gene of BAV-10 was completely sequenced and used in phylogenetic analysis. Based on the results of the phylogenetic analysis, and the location and presence of certain genes thought to be specifically characteristic of subgroup 1 or subgroup 2 BAVs, it could be concluded that, in spite of the striking similarity in certain biological properties, BAV-10 is not related to subgroup 2 BAVs as originally described. It does not however fit clearly into subgroup 1 either, the members of which show closer relationship with human adenoviruses. BAV-10 therefore should best be considered as the first member of a third subgroup of BAVs.
Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.
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Matiz
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Ursu
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Harrach
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Zádori
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Benkö
D006516:Adenoviruses, Canine / Q000235:genetics; D000260:Adenoviruses, Human / Q000235:genetics; D000818:Animals; D000259:Aviadenovirus / Q000235:genetics; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D017921:Mastadenovirus / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012703:Serotyping
D010450:Endopeptidases; C114859:bovine adenovirus type 10 protease