Construction of a stable attenuated Shigella sonnei DeltavirG vaccine strain, WRSS1, and protective efficacy and immunogenicity in the guinea pig keratoconjunctivitis model.
Construction of a stable Shigella sonnei vaccine has been complicated by the instability of the virulence phenotype caused by the spontaneous loss of the invasion plasmid. To select a suitable candidate for vaccine construction, 16 S. sonnei strains were screened for stability of the virulence phenotype. A stable strain, S. sonnei Mosely, was selected for further work. pDeltavirG2, a deletion derivative of the virG gene in the sacB suicide vector pCVD442, was used to generate an S. sonnei virG deletion strain, WRSS1, which was invasive in HeLa cells but negative in the Sereny test. WRSS1 was found to be both immunogenic and protective in the guinea pig keratoconjunctivitis model.
Department of Enteric Infections, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100, USA. dr._antoinette_hartman@wrsmtp-ccmail.army.mil
A B
AB
Hartman
M M
MM
Venkatesan
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001428:Bacterial Vaccines / Q000276:immunology*; D004268:DNA-Binding Proteins / Q000235:genetics*; D004195:Disease Models, Animal; D006168:Guinea Pigs; D006367:HeLa Cells; D006801:Humans; D007637:Keratoconjunctivitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012764:Shigella sonnei / Q000235:genetics / Q000276:immunology*; D014157:Transcription Factors / Q000235:genetics*; D014613:Vaccines, Attenuated / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D014613:Vaccines, Attenuated; D014614:Vaccines, Synthetic; C058483:virG protein, Shigella flexneri