Identification of four chitin synthase genes in the rice blast disease agent Magnaporthe grisea.
Four chitin synthase gene fragments were isolated from Magnaporthe grisea by use of polymerase chain reaction. A pair of degenerate primers based on conserved zymogen-type chitin synthase sequences amplified a approximately 600-bp product containing three chitin synthase gene fragments. A second pair of degenerate primers, based on conserved sequences between the chitin synthases 3 of Saccharomyces cerevisiae and Candida albicans, amplified a single 770-bp fragment. The four corresponding amino acid sequences each fell into one of the chitin synthase classes I-IV, as deduced from sequence analysis. Northern analysis demonstrated that the class II gene was expressed transiently in early phases of growth, whereas the class I and III genes as well as the class IV gene were expressed throughout the entire life cycle. Furthermore, the class III gene was the most expressed.
Laboratoire de Chimie Organique Biologique, URA CNRS 493, Paris, France. vidalc@ccr.jussieu.fr
A
A
Vidal-Cros
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Boccara
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics*; D015152:Blotting, Northern; D015139:Blotting, Southern; D002687:Chitin Synthase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data; D012275:Oryza / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment
D002687:Chitin Synthase