Clofibrate-inducible, 28-kDa peroxisomal integral membrane protein is encoded by PEX11.
We cloned a human PEX11 cDNA by expressed sequence tag homology search using yeast Candida boidinii PEX11, followed by screening of human liver cDNA library. PEX11 encoded a peroxisomal protein Pex11p comprising 247 amino acids, with two transmembrane segments and a dilysine motif at the C-terminus. Pex11p comigrated in SDS-PAGE with a 28-kDa peroxisomal integral membrane protein (PMP28) isolated from the liver of clofibrate-treated rats and was crossreactive to anti-PMP28 antibody, thereby indicating PEX11 to encode PMP28. Pex11p exposes both N- and C-terminal parts to the cytosol. PEX11 was not responsible for ten complementation groups of human peroxisome deficiency disorders.
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan.
I
I
Abe
K
K
Okumoto
S
S
Tamura
Y
Y
Fujiki
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001483:Base Sequence; D016466:CHO Cells; D002994:Clofibrate / Q000494:pharmacology*; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000235:genetics* / Q000276:immunology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000276:immunology; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D000074401:Peroxins; D051381:Rats; D011916:Rats, Inbred F344; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D000906:Antibodies; D017931:DNA Primers; D018076:DNA, Complementary; D005656:Fungal Proteins; D008565:Membrane Proteins; C092128:PEX11 protein, S cerevisiae; C490455:PEX11A protein, human; C490453:PEX11B protein, human; D000074401:Peroxins; C490454:Pex11a protein, rat; D029701:Saccharomyces cerevisiae Proteins; D002994:Clofibrate