DNA extraction from Ascaris suum muscle tissue.
A new method for the extraction of DNA from Ascaris suum muscle has been developed. It combines a standard SDS-based extraction with a plant DNA extraction procedure. The use of SDS and proteinase K allows the elimination of proteins, while CTAB and polyclar AT eliminate glycogen and polyphenols. The DNA thus obtained can easily be digested by endonucleases and amplified by PCR.
Di Mito
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Parasitologie, Institut de zoologie, Université de Neuchâtel, Switzerland.
Betschart
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D000818:Animals; D017165:Ascaris suum / Q000235:genetics*; D000077286:Cetrimonium; D002593:Cetrimonium Compounds; D018509:DNA, Helminth / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI; D004587:Electrophoresis, Agar Gel; D009132:Muscles / Q000737:chemistry; D010274:Parasitology / Q000379:methods; D016133:Polymerase Chain Reaction; D011205:Povidone
D002593:Cetrimonium Compounds; D018509:DNA, Helminth; D015246:Deoxyribonuclease EcoRI; D011205:Povidone; D000077286:Cetrimonium