Alveolar neutrophil oxidative burst and beta2 integrin expression in experimental acute pulmonary inflammation are not modified by inhaled nitric oxide.
It was recently proposed that nitric oxide (NO) inhalation interferes with polymorphonuclear neutrophil (PMN) activation status during acute pulmonary inflammation, although variable results have been observed considering timing of NO administration, species, and model differences. After intratracheal administration of lipopolysaccharide (LPS) in rats, we characterized pulmonary inflammatory reaction (lung wet, dry, and wet to dry weights) and, using flow cytometry, the activation status (H2O2 production and beta2 integrin CD11b/CD18 expression) of PMN obtained from blood and from bronchoalveolar lavage (BAL). Eight hours after LPS injection, rats received for an additional 10 h, at a same Fio2 (85%), either 15 parts per million NO or the same gas flow of nitrogen. We found that 18 h after LPS, lung wet, dry, and wet-to-dry weights, H2O2 production, and CD11b/CD18 expression were increased. PMN obtained from BAL were highly activated as evidenced by an already maximal expression of the beta2 integrin CD11b/CD18, whereas the high H2O2 production at basal state could be further enhanced after ex vivo stimulation. Blood PMN were not different from control cells at basal state; however, their increased capacity to be stimulated ex vivo suggested an in vivo priming effect of intratracheal LPS. In conclusion, inhaled NO, given with a high FiO2, in the presence of this established endotoxinic lung injury did not reverse the markers of PMN activation studied nor lung edema formation in this rat model.
Kermarrec
N
N
Laboratoire de Recherche en Anesthésie Réanimation, CHU Lariboisière, Paris, France.
Chollet-Martin
S
S
Beloucif
S
S
Faivre
V
V
Gougerot-Pocidalo
M A
MA
Payen
D M
DM
D000280:Administration, Inhalation; D000818:Animals; D018821:CD18 Antigens / Q000096:biosynthesis / Q000235:genetics; D004731:Endotoxins / Q000633:toxicity; D004926:Escherichia coli; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000139:chemically induced / Q000503:physiopathology*; D007700:Kinetics; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000473:pathology / Q000502:physiology / Q000503:physiopathology*; D016177:Macrophage-1 Antigen / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D009569:Nitric Oxide / Q000008:administration & dosage / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000276:immunology / Q000503:physiopathology*; D051381:Rats; D016897:Respiratory Burst / Q000187:drug effects
D018821:CD18 Antigens; D004731:Endotoxins; D008070:Lipopolysaccharides; D016177:Macrophage-1 Antigen; D009569:Nitric Oxide