[Comparison of methods to test the "in vitro" cytotoxicity of biocompatible materials].
OBJECTIVE
A comparison of the sensitivity of the agar diffusion method with that of extraction using cell-lines RC-IAL (fibroblastic of rabbit kidney) and HeLa (epithelial carcinoma cells from the cervix uteri of the human uterus), in the in vitro evaluation of materials of medical and hospital.


METHODS
Fifteen samples chosen at random, from among the already known positives and negatives in our stock, were tested and identified as cotton, form, latex, cellulose and acrylic. Besides the samples mentioned, many SDS (GIbco) concentrations were tested experimentally in RC-IAL and HeLa cell cultures.


RESULTS
Of the 50 samples tested, 44(88%) were positive by both methods. However, when the SDS were compared by using the two methods, positive results were noted in the concentrations of from 0.5 to 0.05 microgram/ml in the agar diffusion ans extraction methods. A cytotoxic effect was only noted in the concentrations of up to 0.25 microgram/ml.


CONCLUSIONS
When the SDS was used, differences favorable to the agar diffusion method were observed in the two cell lines, in two concentrations; that is, the sensitivity of this method was quantitatively greater on inspection than that of the extraction method, as well as being the simpler method to use.
Seção de Culturas Celulares do Instituto Adolfo Lutz, São Paulo, Brasil.
A S
AS
Cruz
C A
CA
Figueiredo
T I
TI
Ikeda
A C
AC
Vasconcelos
J B
JB
Cardoso
L F
LF
de Salles-Gomes
D000818:Animals; D001672:Biocompatible Materials / Q000633:toxicity*; D002460:Cell Line; D002584:Cervix Uteri / Q000166:cytology; D004847:Epithelial Cells; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D007668:Kidney / Q000166:cytology; D011817:Rabbits; D012680:Sensitivity and Specificity; D018675:Toxicity Tests / Q000379:methods*
D001672:Biocompatible Materials