Agonist-stimulated glycerophospholipid acyl turnover in alveolar macrophages.
The inflammatory compounds beta-glucan, a particulate agonist, and tannin, a soluble agonist, are present in cotton dust and, when inhaled, cause massive arachidonic acid release from alveolar macrophages. Earlier work had shown that these agonists exhibit different effects on arachidonate liberation and release, and that only tannin inhibits the uptake and incorporation of exogenous arachidonic acid, suggesting inhibition of reacylation. Here we have used the time-dependent incorporation of 18O from H218O-containing media into glycerophospholipid acyl groups as an indicator of acyl turnover in resting and agonist-treated rabbit alveolar macrophages. Highest turnover rates were seen in phosphatidylinositol ( approximately 30% per hour) and in choline phospholipids (10-20% per hour). Both beta-glucan and tannin stimulated acyl turnover, especially arachidonic acid turnover, in these and other lipid classes by a factor of 2 or more. We conclude that neither agonist promotes arachidonic acid accumulation in and release from alveolar macrophages by inhibiting reacylation.
Department of Biochemistry and Thoracic Diseases Research Unit, Mayo Clinic and Foundation, Rochester, MN 55905, USA.
N V
NV
Ralston
P C
PC
Schmid
H H
HH
Schmid
D000215:Acylation / Q000187:drug effects; D000818:Animals; D001095:Arachidonic Acids / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis; D005936:Glucans / Q000494:pharmacology*; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000378:metabolism; D010103:Oxygen Isotopes; D010712:Phosphatidic Acids / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits
D001095:Arachidonic Acids; D005227:Fatty Acids; D005936:Glucans; D047348:Hydrolyzable Tannins; D010103:Oxygen Isotopes; D010712:Phosphatidic Acids