Resident mast cells are important for eotaxin-induced eosinophil accumulation in vivo.
Eotaxin administration intraperitoneally, but not into dorsal air-pouches, of ovalbumin-sensitized mice exhibiting blood eosinophilia induced a threefold increase in eosinophil (E phi s) infiltration. Transfer of 1 x 10(6) mixed peritoneal cavity cells (PCC), containing 3.5 to 4.5 x 10(4) mast cells (MC), from donor mice to air-pouches of sensitized (but not unsensitized) recipient mice, established an E phi infiltration to eotaxin (vehicle, 0.86 +/- 0.27 x 10(6); eotaxin, 1.63 +/- 0.16 x 10(6) E phi s/air-pouch). Neutrophil numbers were also increased. When MC-depleted (-93%) PCC were injected into air-pouches of recipient animals, E phi infiltration was not supported (-52%). Injection of macrophage-depleted (-99%) PCC into air-pouches elicited a full E phi response to eotaxin but not neutrophil infiltration (-81%). Systemic dexamethasone treatment of recipient mice reduced E phi accumulation; treatment of donor mice only reduced neutrophil accumulation. Our study points to a crucial role for MC in E phi recruitment by eotaxin.
Department of Biochemical Pharmacology, William Harvey Research Institute, London, United Kingdom. a.m.das@mds.qmw.ac.uk
A M
AM
Das
R J
RJ
Flower
M
M
Perretti
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology*; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil / Q000494:pharmacology*; D016207:Cytokines / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D004342:Drug Hypersensitivity / Q000276:immunology; D004802:Eosinophilia / Q000276:immunology; D004804:Eosinophils / Q000166:cytology* / Q000276:immunology; D005260:Female; D007958:Leukocyte Count; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology* / Q000637:transplantation; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000166:cytology; D011994:Recombinant Proteins / Q000494:pharmacology; D012867:Skin / Q000166:cytology / Q000276:immunology
D000893:Anti-Inflammatory Agents; C515457:Ccl11 protein, mouse; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002631:Chemotactic Factors, Eosinophil; D016207:Cytokines; D011994:Recombinant Proteins; D003907:Dexamethasone; D010047:Ovalbumin