Novel method for rapid measurement of growth of mycobacteria in detergent-free media.
We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.
Meyers
P R
PR
Department of Medical Microbiology, University of Cape Town, Observatory, Cape Town 7925, South Africa.
Bourn
W R
WR
Steyn
L M
LM
van Helden
P D
PD
Beyers
A D
AD
Brown
G D
GD
D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D003470:Culture Media; D003902:Detergents; D009161:Mycobacterium / Q000254:growth & development*; D009163:Mycobacterium bovis / Q000254:growth & development*; D009169:Mycobacterium tuberculosis / Q000254:growth & development*; D012972:Sodium Hydroxide; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D003902:Detergents; D012972:Sodium Hydroxide