Transformation of the phytopathogen Mycosphaerella graminicola to carbendazim and hygromycin B resistance.
The fungal wheat pathogen Mycosphaerella graminicola was transformed to carbendazim and hygromycin B resistance. A beta-tubulin gene from a M. graminicola strain resistant to the fungicide carbendazim was cloned and used to transform a sensitive strain to carbendazim resistance. Hygromycin B-resistant transformants (up to 8 per microgram of transforming DNA) arose at a higher rate than beta-tubulin transformants (up to 0.6 per microgram of DNA). Transformants were able to infect wheat and were morphologically similar to recipient strains.
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS41 9AF, UK. Andrew.Payne@BBSRC.AC.UK
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Payne
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MC
Grosjean-Cournoyer
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Hollomon
D000935:Antifungal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects* / Q000235:genetics* / Q000472:pathogenicity; D001483:Base Sequence; D001562:Benzimidazoles / Q000494:pharmacology*; D002219:Carbamates; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005800:Genes, Fungal; D005821:Genetic Techniques; D006921:Hygromycin B / Q000494:pharmacology*; D014170:Transformation, Genetic; D014908:Triticum / Q000382:microbiology; D014404:Tubulin / Q000235:genetics
D000935:Antifungal Agents; D001562:Benzimidazoles; D002219:Carbamates; D017931:DNA Primers; D004271:DNA, Fungal; D014404:Tubulin; D006921:Hygromycin B; C006698:carbendazim