Cyclooxygenase-2 expression in human adenocarcinoma cell line HT29 cl.19A.
BACKGROUND
Overexpression of cyclooxygenase-2 (COX-2) has been implicated in carcinogenesis of human colorectal cancer which is one of the leading types of cancer in Western countries.


METHODS
We analyzed the COX-2 expression and activity using RT-PCR, Western blot, immunocytochemistry, RP-HPLC and EIA analysis in 0% and 10% fetal bovine serum (FBS) cultured cells.


RESULTS
HT 29 cl.19A cells exhibited a COX-2 expression called "constitutive" in the absence of FBS in culture media. This particular expression was not the result of a mutation of the HT29 cl.19A COX-2 gene promotor.


CONCLUSIONS
In our study, the human colon adenocarcinoma cell line, HT29 cl.19A, expressed COX-2 abnormally. This expression appeared to be at the same time inducible by the action of classical exogenous inducers such as FBS or interleukin-1 beta and "constitutive" if none of these compounds were present.
Laboratoires de Biochimie, Facultés de Médecine et de Pharmacie, Limoges, France.
S
S
Battu
H
H
Chable-Rabinovitch
M
M
Rigaud
J L
JL
Beneytout
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002417:Cattle; D002455:Cell Division; D003001:Cloning, Molecular; D015179:Colorectal Neoplasms / Q000201:enzymology; D003470:Culture Media; D016895:Culture Media, Serum-Free; D051546:Cyclooxygenase 2; D017931:DNA Primers; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000096:biosynthesis* / Q000235:genetics; D008565:Membrane Proteins; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis
D003470:Culture Media; D016895:Culture Media, Serum-Free; D017931:DNA Primers; D007527:Isoenzymes; D008565:Membrane Proteins; D011994:Recombinant Proteins; D016718:Arachidonic Acid; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases