Detection of mumps virus in clinical specimens by rapid centrifugation culture and conventional tube cell culture.
Conventional tube cell culture was compared with a 2 day and 5 day spin-amplified shell vial indirect immunofluorescence assay for the detection of mumps virus in swabs from the area of Stensen's duct. The sensitivity and specificity of the shell vial assay were 95.9 and 100%, respectively. The shell vial detected 66.3% of the positive cultures within 2 days of inoculation while the first positive results were available by conventional tube cell culture after 3 days (1.6%) reaching 72.4% of all culture positive specimens after 7 days. These data suggest that a centrifugation shell vial indirect immunofluorescence assay may be useful for rapid detection of mumps virus in clinical specimens.
Institute of Medical Microbiology, Division of Clinical Microbiology, University of Bern, Switzerland. sisa@ms1.kssg.ch
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Germann
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Gorgievski
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Ströhle
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Matter
D000818:Animals; D000911:Antibodies, Monoclonal; D002498:Centrifugation; D002648:Child; D002522:Chlorocebus aethiops; D003588:Cytopathogenic Effect, Viral; D005260:Female; D016650:Fluorescein-5-isothiocyanate; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D009107:Mumps / Q000175:diagnosis* / Q000821:virology; D009109:Mumps virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D018987:Salivary Ducts / Q000821:virology*; D012680:Sensitivity and Specificity; D014709:Vero Cells; D014776:Virus Cultivation / Q000379:methods*
D000911:Antibodies, Monoclonal; D016650:Fluorescein-5-isothiocyanate