Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997.
The ISOBM TD-6 Workshop is the first international workshop on monoclonal antibodies against the Sialyl Lewisa (SLea) antigen. Eight research groups participated in a blind study to characterize the epitope binding, relative affinity and performance in immunoradiometric assays, of a panel of 20 monoclonal antibodies. The antibodies were tested against a diverse panel of neoglycoconjugates, purified antigens and human serum pools from gastrointestinal malignancies. Epitope specificities were determined for the majority of antibodies in the panel. Cross-reactivity with related saccharide structures was noted in several antibodies. Overall, the results of the TD-6 Workshop show further development of SLea immunoassays may yield yet more specific assays for the detection and management of gastrointestinal and other malignancies.
Central Laboratory, Norwegian Radium Hospital, Montebello, Oslo, Norway.
P D
PD
Rye
N V
NV
Bovin
E V
EV
Vlasova
A A
AA
Molodyk
A
A
Baryshnikov
F T
FT
Kreutz
W I
WI
Garinther
B C
BC
Schultes
A A
AA
Noujaim
R
R
Madiyalakan
J
J
Magnani
O
O
Nilsson
K
K
Nilsson
K
K
Nustad
L
L
Norum
H
H
Bell
Y
Y
Cao
M R
MR
Suresh
D L
DL
Very
J V
JV
Freeman
K K
KK
Yeung
J
J
Hilgers
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis*; D018395:CA-19-9 Antigen / Q000276:immunology / Q000378:metabolism; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000276:immunology; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005770:Gastrointestinal Neoplasms / Q000097:blood; D006801:Humans; D015592:Immunoradiometric Assay; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D000939:Epitopes; D005732:Gangliosides; C064564:sialyl Le(a) ganglioside