Aminoacyl-tRNA analogues; synthesis, purification and properties of 3'-anthraniloyl oligoribonucleotides.
Reaction of isatoic anhydride with adenosine, adenosine 5'-phosphate, oligoribonucleotides or with the E. coli tRNAVal led to attachment of an anthraniloyl residue at 2'- or 3'-OH groups of 3'-terminal ribose residue. No protection of the 5'-hydroxyl group or internal 2'-hydroxyl groups is required for this specific reaction. Anthraniloyl-tRNA which is an analogue of aminoacyl-tRNA forms a ternary complex with EF-Tu*GTP. The anthraniloyl-residue is used as a fluorescent reporter group to monitor interactions with proteins.
Nawrot
B
B
Laboratorium für Biochemie, Universität Bayreuth, Germany. bnawrot@lodz.pdi.net
Sprinzl
M
M
D001483:Base Sequence; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification / Q000494:pharmacology; D012329:RNA, Bacterial / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D062367:ortho-Aminobenzoates / Q000737:chemistry
D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; D062367:ortho-Aminobenzoates; C031385:anthranilic acid