Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP).
Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.
Asaeda
A
A
Department of Veterinary Radiology, School of Veterinary Medicine, College of Agriculture, Osaka Prefecture University, Japan.
Ide
H
H
Tano
K
K
Takamori
Y
Y
Kubo
K
K
D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000447:Aldehydes / Q000032:analysis; D001710:Biotin / Q000031:analogs & derivatives; D019757:Carbon-Oxygen Lyases / Q000378:metabolism; D004247:DNA / Q000737:chemistry*; D045647:DNA Glycosylases; D004260:DNA Repair / Q000235:genetics*; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D043223:Deoxyribonuclease IV (Phage T4-Induced); D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D016296:Mutagenesis / Q000235:genetics; D009699:N-Glycosyl Hydrolases / Q000737:chemistry*; D011994:Recombinant Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured
D000447:Aldehydes; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D001710:Biotin; D004247:DNA; D008741:Methyl Methanesulfonate; D043223:Deoxyribonuclease IV (Phage T4-Induced); D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D019757:Carbon-Oxygen Lyases; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D000225:Adenine