Isolation and functional analysis of the histone H3 promoter from atlantic salmon (Salmo salar L.).
The histone H3 (sH3) promoter of Atlantic salmon (Salmo salar) was cloned via polymerase chain reaction using primers designed from the rainbow trout (Oncorhynchus mykiss) promoter sequence. A comparison of the nucleotide sequence with the equivalent sequences from rainbow trout and sockeye salmon (Oncorhynchus nerka) revealed a high degree of conservation. In vivo expression analysis of the sH3 promoter was carried out in both rainbow trout and zebrafish (Danio rerio) embryos. A direct comparison of the sH3 promoter with the viral RSV promoter in rainbow trout resulted in stronger expression of the sH3 promoter. Furthermore, lacZ expression directed by the sH3 promoter was ubiquitous in several different cell types in developing zebrafish embryos. These results suggest that the sH3 promoter will be useful in transgenic studies in Atlantic salmon.
National Diagnostics Centre, BioResearch Ireland, National University of Ireland, Galway, Ireland.
S
S
Hanley
T J
TJ
Smith
F
F
Muller
N
N
Maclean
S
S
Uzbekova
P
P
Prunet
B
B
Breton
D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D004625:Embryo, Nonmammalian / Q000378:metabolism; D006657:Histones / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D017683:Oncorhynchus / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012474:Salmon / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015027:Zebrafish / Q000196:embryology; D001616:beta-Galactosidase / Q000096:biosynthesis
D017931:DNA Primers; D006657:Histones; D011993:Recombinant Fusion Proteins; D001616:beta-Galactosidase