Effects of growth-promoting antimicrobials on inhibition of Yersinia enterocolitica O:3 by porcine ileal microflora.
The survival of Yersinia enterocolitica serotype O:3 was examined in an in vitro model of the porcine ileum in the presence of normal ileal microflora using a medium supplemented with the growth-promoting antimicrobials avoparcin, spiramycin, tylosin, carbadox and olaquindox, or without any antimicrobials, at 39 degrees C for 5 d. Growth of Y. enterocolitica was inhibited in all trials in the following order: avoparcin < carbadox < spiramycin < no feed additives < tylosin < olaquindox. The media supplemented with avoparcin, carbadox and spiramycin supported the survival of Y. enterocolitica up to 5 d. When incubated with the normal ileal microflora without any growth-promoting antimicrobials, Y. enterocolitica could not be isolated after 4 d. With olaquindox and tylosin, Y. enterocolitica was not detected after 3 d.
Asplund
K
K
National Veterinary and Food Research Institute, Helsinki, Finland.
Hakkinen
M
M
Okkonen
T
T
Vanhala
P
P
Nurmi
E
E
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D006020:Glycopeptides; D007082:Ileum / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D014066:Palatine Tonsil / Q000382:microbiology; D011810:Quinoxalines / Q000494:pharmacology; D013552:Swine / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D006020:Glycopeptides; D011810:Quinoxalines; C013168:olaquindox; C016249:avoparcin