Ascorbic acid prevents beta-amyloid-induced intracellular calcium increase and cell death in PC12 cells.
Characteristics of Alzheimer's disease include loss of brain neurons associated with the deposit of beta-amyloid protein (A beta) which is thought to be toxic to neurons possibly via induction of intracellular calcium and generation of free radicals. On this basis, we have determined the effect of ascorbic acid on the cell death and calcium increase induced by A beta in PC12 cells. We found that ascorbic acid completely abolished A beta-induced calcium increase and cell death in PC12 cells, indicating that calcium elevation and cell death are associated phenomena induced by A beta that can be rescued by antioxidants. These results are important to understand the mechanisms by which A beta is toxic to neurons and suggest that antioxidants may be part of future treatments for Alzheimer's disease.
Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch at Galveston, 77555-0652, USA.
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Yallampalli
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Micci
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Taglialatela
D016229:Amyloid beta-Peptides / Q000037:antagonists & inhibitors*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism*; D016716:PC12 Cells / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D014407:Tumor Cells, Cultured
D016229:Amyloid beta-Peptides; D001205:Ascorbic Acid; D002118:Calcium