11Beta-hydroxysteroid dehydrogenase expression in first trimester human trophoblasts.
This study evaluated the levels and the enzymatic characteristics of 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) of chorionic villi isolated from first trimester human placenta. The results demonstrated a predominant expression of the NAD-dependent dehydrogenase isoform (11beta-HSD2) over the NADP-dependent oxoreductase (11beta-HSD1). Thus, in tissue homogenates exogenous NAD increased the conversion of corticosterone to 11-dehydrocorticosterone of about 14-fold while NADP was ineffective. There was no conversion of 11-dehydrocorticosterone to corticosterone either with NADH or NADPH demonstrating the lack of reductase activity. In keeping with these results, RT-PCR analysis indicated a mRNA for 11beta-HSD2 in villous tissue while 11beta-HSD1 mRNA levels were undetectable. In addition, immunohistochemical staining localized the 11beta-HSD2 protein to syncytiotrophoblasts and cell columns of the chorionic villi. These results suggest roles for the trophoblast-associated 11beta-HSD2 oxidative activity in modulating the exposure of the embryo to active glucocorticoids in the early gestation and in regulating trophoblasts invasion of the uterine wall.
Arcuri
F
F
Institute of Pathological Anatomy and Histology, University of Siena, Italy.
Sestini
S
S
Paulesu
L
L
Bracci
L
L
Carducci
A
A
Manzoni
F
F
Cardone
C
C
Cintorino
M
M
D042842:11-beta-Hydroxysteroid Dehydrogenases; D015153:Blotting, Western; D002824:Chorionic Villi / Q000201:enzymology; D003345:Corticosterone / Q000378:metabolism; D005260:Female; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis / Q000235:genetics* / Q000378:metabolism*; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009928:Organ Specificity; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000201:enzymology*
D007527:Isoenzymes; D012333:RNA, Messenger; D009243:NAD; D009249:NADP; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D003345:Corticosterone