Norepinephrine-augmenting lipolytic effectors from Astilbe thunbergii rhizomes.
An EtOAc-soluble fraction from a 80% Me2CO extract of the rhizomes of Astilbe thunbergii enhanced norepinephrine-induced lipolysis in rat fat cells, while an EtOAc-insoluble fraction had no effect. The active substances isolated from the EtOAc-soluble fraction of the rhizomes were identified as eucryphin (1), bergenin (2), and astilbin (3), which enhanced norepinephrine-induced lipolysis at concentrations of 10-1000 microgram/mL, while they themselves did not cause lipolysis. Furthermore, these compounds slightly stimulated adrenocorticotrophic hormone-induced lipolysis and inhibited insulin-induced lipogenesis from glucose.
Second Department of Medical Biochemistry, School of Medicine, Ehime University, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan.
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Han
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Ninomiya
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Taniguchi
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Baba
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Kimura
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Okuda
D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D002240:Carbohydrate Sequence; D002681:China; D008066:Lipolysis / Q000187:drug effects*; D008297:Male; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012462:Saline Solution, Hypertonic
D000316:Adrenergic alpha-Agonists; D010936:Plant Extracts; D012462:Saline Solution, Hypertonic; D009638:Norepinephrine