Correlation between protein flexibility and electron transfer from QA-* to QB in PSII membrane fragments from spinach.
To analyze a possible correlation between the extent of QA-* reoxidation and protein dynamics, fluorometric and Mössbauer spectroscopic measurements were performed in photosystem II membrane fragments from spinach. Numerical evaluation of the flash-induced change of the normalized fluorescence quantum yield revealed that the extent of reoxidation starts to decrease below 275 K and is almost completely suppressed at 230 K. Detailed analyses of Mössbauer spectra measured at different temperatures in 57Fe-enriched material indicate that the onset of fluctuations between conformational substates of the protein matrix occurs also at around 230 K. Based on this correspondence, protein flexibility is inferred to play a key role for QA-* reoxidation in photosystem II. Taking into account the striking similarities with purple bacteria and the latest structural information on these reaction centers [Stowell, M. H. B., McPhillips, T. M., Rees, D. C., Soltis, S. M., Abresch, E., and Feher, G. (1997) Science 276, 812-816], it appears most plausible that also the headgroup of plastoquinone-9 bound to the QB-site in PSII requires a structural reorientation for its reduction to the semiquinone.
Garbers
A
A
Physik Department, Technische Universität München, Garching, Germany.
Reifarth
F
F
Kurreck
J
J
Renger
G
G
Parak
F
F
D002736:Chloroplasts / Q000737:chemistry / Q000378:metabolism; D004579:Electron Transport; D007425:Intracellular Membranes / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D012247:Rhodospirillum rubrum; D015204:Spectroscopy, Mossbauer; D018724:Spinacia oleracea; D013329:Structure-Activity Relationship; D013696:Temperature; D013816:Thermodynamics
D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex