Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II.
The microsome of insect cells expressing CYP Ge-5 (CYP93B1), a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), catalyzed the formation of [14C]licodione and [14C]2-hydroxynaringenin from (2S)-[14C]liquiritigenin and (2S)-[14C]naringenin, respectively. On acid treatment, the products were converted to 14C-labeled 7,4'-dihydroxyflavone and apigenin. Eriodictyol was also converted to luteolin by the reaction with the microsome of yeast expressing CYP93B1 and subsequent acid treatment. CYP93B1 was thus shown to encode (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II depending on the substrates employed.
Akashi
T
T
Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa, Japan.
Aoki
T
T
Ayabe
S
S
D000818:Animals; D002478:Cells, Cultured; D002599:Chalcone / Q000031:analogs & derivatives / Q000378:metabolism; D003001:Cloning, Molecular; D003577:Cytochrome P-450 Enzyme System; D006035:Glycyrrhiza / Q000201:enzymology* / Q000235:genetics; D008861:Microsomes / Q000378:metabolism; D006899:Mixed Function Oxygenases; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D012118:Resorcinols / Q000378:metabolism; D012441:Saccharomyces cerevisiae; D018411:Spodoptera
D012118:Resorcinols; D002599:Chalcone; C105003:licodione; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C114032:cytochrome P-450 CYP93B1 (Glycyrrhiza echinata)