Sterically stabilized phospholipids attenuate human neutrophils chemotaxis in vitro.
The purpose of this study was to determine whether sterically stabilized liposomes (SSL) and poly(ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) attenuate polymorphonuclear neutrophils (PMNs) chemotaxis in vitro and, if so, whether incorporation of vasoactive intestinal peptide (VIP), a pleiotropic neuropeptide, on the surface of SSL amplifies SSL-induced responses. Using a modified blind-well chamber chemotaxis assay, we found that N-formyl-methionyl-leucyl-phenylalanine (FMLP; 0.1 microM) and zymosan opsonized with purified human complement (2 x 10(9) yeast wall particles/ml) elicit significant human PMNs chemotaxis (95+/-9 and 103+/-3 cells/high power field; p<0.05). These effects are significantly attenuated by SSL and PEG-DSPE (p<0.05). By contrast, aqueous VIP and VIP on SSL have no significant effects on FMLP- and zymosan-induced responses. We conclude that certain sterically stabilized liposomes and phospholipids attenuate human PMNs chemotaxis in vitro and that VIP does not modulate this response.
Hatipoglu
U
U
Department of Medicine, University of Illinois at Chicago, and West Side Department of Veterans Affairs Medical Center, 60612-7323, USA.
Gao
X
X
Verral
S
S
Séjourné
F
F
Pitrak
D
D
Alkan-Onyüksel
H
H
Rubinstein
I
I
D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003165:Complement System Proteins; D004355:Drug Stability; D006801:Humans; D008081:Liposomes; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D009895:Opsonin Proteins; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010743:Phospholipids / Q000008:administration & dosage / Q000494:pharmacology*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000008:administration & dosage; D015054:Zymosan / Q000494:pharmacology
D008081:Liposomes; D009895:Opsonin Proteins; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C107570:polyethylene glycol-distearoylphosphatidylethanolamine; D014660:Vasoactive Intestinal Peptide; D011092:Polyethylene Glycols; D009240:N-Formylmethionine Leucyl-Phenylalanine; D003165:Complement System Proteins; D015054:Zymosan