Effect of PACAP-27 on 14C-aminopyrine accumulation in isolated rabbit parietal cells.
Pituitary adenylate cyclase-activating polypeptide (PACAP) is present in gastric mucosa, but its direct effect on parietal cells is unknown. We examined this using 14C-aminopyrine uptake in elutriated rabbit cells. PACAP-27 had no effect on basal cells but significantly increased the response to histamine (10(-4) M) at 10(-9) M and to carbachol (10(-4) M) in the presence of ranitidine (10(-4) M) at 10(-7) M and 10(-8) M. PACAP (6-38), an antagonist of PACAP, inhibited the effect of PACAP-27 on carbachol-stimulated cells. Vasoactive intestinal peptide had no significant effect. In conclusion, PACAP-27 has a direct additive effect on stimulated rabbit parietal cells in vitro.
Department of Medicine, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom. zhealey@rpms.ac.uk
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Healey
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Bliss
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Edwards
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Arebi
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Beales
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Calam
D000632:Aminopyrine / Q000378:metabolism*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D002478:Cells, Cultured; D006632:Histamine / Q000494:pharmacology; D009479:Neuropeptides / Q000494:pharmacology*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010295:Parietal Cells, Gastric / Q000187:drug effects* / Q000378:metabolism; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D011817:Rabbits; D011899:Ranitidine / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D009479:Neuropeptides; D018377:Neurotransmitter Agents; D051219:Pituitary Adenylate Cyclase-Activating Polypeptide; D000632:Aminopyrine; D014660:Vasoactive Intestinal Peptide; D006632:Histamine; D011899:Ranitidine; D002217:Carbachol