[A comparison of the diagnostic value of the virus neutralization reaction and of indirect immunoenzyme analysis using monoclonal antibodies for the intratype differentiation of vaccinal and virulent poliovirus strains].
A comparative evaluation was carried out of potentialities of the practical use of the virus neutralization reaction (VNR) and indirect immunoenzymatic analysis (IIEA) relying on the monoclonal antibodies (MKAB) for intratype differentiation of polioviruses. It has been proved that in each of the reactions MKAB can interact with different epitopes on the surface of virions. This brings about an unequal degree of activity and specificity of similar antibodies in the two serologic reactions. In VNR monoclonal antibodies "work" in a more specific manner than they do in IIEA. Therefore this test is still more suitable for clinical use in intratype differentiation of polioviruses.
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Nikolaienko
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000956:Antigens, Viral / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D007124:Immunoenzyme Techniques; D009500:Neutralization Tests / Q000379:methods; D017955:Poliovirus / Q000145:classification* / Q000276:immunology; D011054:Poliovirus Vaccine, Inactivated / Q000145:classification* / Q000276:immunology; D012703:Serotyping / Q000379:methods; D014774:Virulence / Q000276:immunology
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D000939:Epitopes; D011054:Poliovirus Vaccine, Inactivated