Insulin receptor-related receptor in rat islets of Langerhans.
The physiological aspects and roles of the insulin receptor-related receptor (IRR), a novel member of the insulin receptor family, are unknown. Published work shows a characteristic localization of IRR mRNA at the cellular level in the kidney, stomach and brain. In our previous study, a specific antibody against the rat IRR beta subunit was generated and used to identify the IRR-expressing cells as B-type intercalated cells in the kidney. In this study. IRR cDNA was amplified by PCR to demonstrate IRR mRNA expression in the rat pancreas. Immunohistochemical analysis with the anti-IRR antibody revealed that IRR immunoreactivity is localized in the islets of Langerhans. The IRR-immunoreactive cells were most likely to be the islet B cells which were stained with anti-insulin antibody. This is the first finding on the involvement of IRR in the physiological functions of insulin-secreting cells.
Department of Genetic Biochemistry, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
K
K
Ozaki
D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011972:Receptor, Insulin / Q000032:analysis* / Q000235:genetics
D018076:DNA, Complementary; D012333:RNA, Messenger; D011972:Receptor, Insulin; C066542:insulin receptor-related receptor