Proadrenomedullin N-terminal 20 peptide (PAMP) stimulates aldosterone secretion by the intact rat adrenal glomerulosa by a cAMP-dependent mechanism.
Preproadrenomedullin, the precursor of proadrenomedullin N-terminal 20 peptide (PAMP), is produced by rat zona glomerulosa cells. The actions of PAMP on the rat adrenal have been investigated. PAMP was found to stimulate aldosterone secretion and cAMP release by intact capsules, in a dose-dependent manner, but had only a minor effect on dispersed cells. The effects of PAMP on aldosterone secretion were inhibited by HA1004, an inhibitor of protein kinase A. The difference between tissue preparations does not appear to be due to the actions of PAMP on local release of catecholamines as PAMP inhibited the release of catecholamines from rat capsular preparations. These data suggest that PAMP is a novel zona glomerulosa stimulant in intact capsular tissue, acting through cAMP.
Department of Biochemistry, St. Bartholomew's and the Royal London School of Medicine and Dentistry, Queen Mary and Westfield College, UK.
L M
LM
Thomson
S
S
Kapas
J P
JP
Hinson
D053607:Adrenomedullin; D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D005260:Female; D007546:Isoquinolines / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D010455:Peptides; D011498:Protein Precursors / Q000494:pharmacology*; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013449:Sulfonamides; D015384:Zona Glomerulosa / Q000187:drug effects*
D002395:Catecholamines; D007546:Isoquinolines; D010446:Peptide Fragments; D010455:Peptides; D011498:Protein Precursors; D011506:Proteins; D013449:Sulfonamides; D053607:Adrenomedullin; D000450:Aldosterone; C043204:N-(2-guanidinoethyl)-5-isoquinolinesulfonamide; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases