Antigenic characterization of HIV-1 gp41 binding proteins.
The envelope protein HIV-1 gp41 has been shown to exert various effects on human T-cells, B-cells and monocytes like inhibition of cell proliferation, modulation of MHC expression and cytokine production. In contrast to gpl20, where several receptor molecules have been identified, the receptor for gp41 is still unknown. Using a sepharose column, coupled with recombinant soluble gp41, (rsgp41; Env amino acids 539-684), five gp41-binding proteins of 37, 45, 50, 62 and 100 kDa had been isolated from lysates of the B-cell line Raji. Two mouse antiserums were generated against the proteins P45 and P62 and were tested against the binding specificity of both antiserums. In Western blot analysis the antiserums recognized two protein bands of 45 and 62 kDa in complete Raji cell lysates, as well as the purified proteins P45 and P62, respectively, but did not show any cross-reaction, indicating that the two proteins do not share any immunological epitopes. Besides, the polyclonal antiserums did not recognize the other gp41-binding proteins P37, P50 and P100. Using the P62 antiserum proteins of the same size as in Raji cell lysates were stained in the lysates of the monocytic cell line U937 and the T-cell line H9, demonstrating distribution of P62 in different blood cells. P45 seems not to be identical to HLA-C which had been shown to bind to gp41. These results indicate, that P45 and P62 are two separate gp41-binding proteins without homology to each other or to the other gp41-binding proteins.
Chen
Y H
YH
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, People's Republic of China.
Speth
C
C
Wu
W
W
Stockl
G
G
Xiao
Y
Y
Yu
T
T
Ke
Z
Z
Zhao
Y
Y
Dierich
M P
MP
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology*; D015700:HIV Envelope Protein gp41 / Q000378:metabolism*; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015728:Receptors, HIV / Q000276:immunology*; D014407:Tumor Cells, Cultured
D000954:Antigens, Surface; D015700:HIV Envelope Protein gp41; D008565:Membrane Proteins; D015728:Receptors, HIV