Characterization of a galactose-1-phosphate uridylyltransferase gene from the marine red alga Gracilaria gracilis.
The metabolism of D-galactose is a major feature of red-algal physiology. We have cloned and sequenced a gene from the red alga Gracilaria gracilis that encodes a key enzyme of D-galactose metabolism, galactose-1-phosphate uridylyltransferase (GALT). This gene, designated GgGALT1, is apparently devoid of introns. A potential TATA box, four potential CAAT boxes, and a repeated sequence occur in the 5'-flanking region. The predicted 369-aa peptide shares significant sequence similarity with GALTs from other organisms (human, 47%; Saccharomyces cerevisiae, 49%; Solanum tuberosum, 49%). Southern-hybridization analysis reveals two related, but apparently not identical, GALT genes in the nuclear genome of G. gracilis. Sequence analysis indicates that the GgGALT1 enzyme lacks a rubredoxin "knuckle" motif, which in bacterial and fungal GALTs is involved in binding zinc. An open reading frame encoding a potential peptidyl tRNA hydrolase occurs 179 bp downstream from the GgGALT1 gene.
Lluisma
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Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1 and Department of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1.
Ragan
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain / Q000235:genetics; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000461:Rhodophyta / Q000201:enzymology* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004247:DNA; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase