Molecular diagnostics of clinical strains of filamentous Basidiomycetes.
Eleven clinical and veterinary strains of filamentous Basidiomycetes were compared with 15 reference strains representing the orders Aphyllophorales and Agaricales. The methods used were restriction analysis of small subunit (18S) (SSU) rDNA and internal transcribed spacers (ITS) 1 and 2 and variable domain 9 (V9)-ITS1 sequencing. Six strains were found to belong to the teleomorph genera Schizophyllum or Coprinus, whereas five could not be identified unequivocally. A rapid diagnostic overview is obtained with HaeIII and HinfI digestion of the ITS region.
Centraalbureau voor Schimmelcultures CBS, Baarn, The Netherlands.
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Gerrits van den Ende
D000363:Agaricales / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D000818:Animals; D001483:Base Sequence; D001487:Basidiomycota / Q000145:classification* / Q000235:genetics / Q000472:pathogenicity; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009181:Mycoses / Q000175:diagnosis* / Q000662:veterinary; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S