The regulation of galanin gene expression in gonadotropin-releasing hormone neurons.
In rats, galanin modulates luteinizing hormone (LH) secretion, and gonadotropin-releasing hormone (GnRH) neurons provide a possible source of this galanin. To understand galanin's physiological role in GnRH neurons, we used double-label in situ hybridization and computerized image analysis to examine the regulation of galanin message in GnRH neurons. We found that galanin gene expression in GnRH neurons is regulated by sex steroids, induced coincident with the LH surge, and persists well after the completion of the LH surge, and that the induction of galanin message in GnRH neurons coincident with the LH surge is sexually differentiated neonatally. We postulate that the rise in galanin gene expression in GnRH neurons at the time of the LH surge serves to replenish galanin released with GnRH that is needed for the production of the LH surge, or that galanin is involved in physiological events that occur subsequent to the LH surge.
Finn
P D
PD
Department of Physiology, University of Washington, Seattle 98195, USA.
Clifton
D K
DK
Steiner
R A
RA
D000818:Animals; D004971:Estrus / Q000502:physiology; D005260:Female; D019004:Galanin / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D005786:Gene Expression Regulation; D012739:Gonadal Steroid Hormones / Q000502:physiology; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D051381:Rats
D012739:Gonadal Steroid Hormones; D007987:Gonadotropin-Releasing Hormone; D019004:Galanin; D007986:Luteinizing Hormone