Profile of protein kinase C isozymes and their possible role in mammalian egg activation.
Western blot analysis was used to investigate protein kinase C (PKC) profile of rat eggs. The presence of eight PKC isozymes was demonstrated: conventional PKC alpha, beta and gamma; novel PKC delta, epsilon and mu; atypical PKC zeta and lambda. PKC alpha was detected by RT-PCR as well. PKC translocation from the cytosol to the plasma membrane served as a marker for enzyme activation. Immunofluorescence confocal microscopy demonstrated a relatively uniform distribution of PKC alpha, betaI, and betaII throughout the cytosol of metaphase II arrested eggs. PKC accumulation at the plasma membrane was detected 5 min after exposure to 12-O-tetradecanoyl phorbol-13-acetate and increased with time, thus demonstrating activation of these PKCs.
Department of Embryology and Teratology, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, Israel.
T
T
Raz
E
E
Eliyahu
V
V
Yesodi
R
R
Shalgi
D000818:Animals; D001483:Base Sequence; D001692:Biological Transport; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D017931:DNA Primers; D004789:Enzyme Activation; D005260:Female; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000378:metabolism*; D010063:Ovum / Q000201:enzymology* / Q000502:physiology; D011493:Protein Kinase C / Q000378:metabolism*; D051381:Rats
D017931:DNA Primers; D007527:Isoenzymes; D011493:Protein Kinase C