An alpha-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-->3)-linked fucosyl oligosaccharides by transglycosylation.
A new alpha-L-fucosidase was partially purified from the culture broth of Penicillium multicolor, which was available commercially as a freeze dried powder by the name of Lactase-P. This enzyme catalysed the transglycosylation of fucose residue of p-nitrophenyl-alpha-L-fucopyranoside to give alpha-L-Fuc-(1-->3)-D-Glc or alpha-L-Fuc-(1-->3)-D-GlcNAc regioselectively. This enzyme was more stable in the organic co-solvents than the alpha-fucosidase from Aspergillus niger, which was also proposed previously by us as an enzyme to produce fucosyl oligosaccharides.
Meiji Institute of Health Science, Meiji Milk Products, Co., Ltd., Odawara, Japan. rxg0120@niftyserve.or.jp
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Ajisaka
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Fujimoto
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Miyasato
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000096:biosynthesis; D006031:Glycosylation; D007700:Kinetics; D008970:Molecular Weight; D010407:Penicillium / Q000201:enzymology*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012997:Solvents; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000302:isolation & purification / Q000378:metabolism*
D004187:Disaccharides; D011135:Polysaccharides, Bacterial; D012997:Solvents; D005644:alpha-L-Fucosidase