DOC-2/hDab2, a candidate tumor suppressor gene involved in the development of gestational trophoblastic diseases.
Gestational trophoblastic diseases comprise a spectrum of interrelated diseases including partial mole, complete mole and gestational choriocarcinoma. Using reverse transcriptase PCR (RT-PCR) analysis, we identified higher levels of DOC-2/hDab2 expression in the normal trophoblast cells in culture than in choriocarcinoma cell lines. Subsequent study using immunohistochemistry showed high levels of DOC-2/hDab2 protein expression in normal trophoblast tissues but significantly lower levels of expression in gestational trophoblastic disease tissues, particularly in complete mole and choriocarcinoma. When DOC-2/hDab2 was transfected into the choriocarcinoma cell lines, Jar, JEG and BeWo, the stable transfectants showed significantly reduced growth rate in culture. These data suggest that down regulation of DOC-2/hDab2 may play an important role in the development of gestational trophoblastic diseases.
Department of Obstetrics, Gynecology and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
V
V
Fulop
C V
CV
Colitti
D
D
Genest
R S
RS
Berkowitz
G K
GK
Yiu
S W
SW
Ng
J
J
Szepesi
S C
SC
Mok
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D000595:Amino Acid Sequence; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D002455:Cell Division; D002460:Cell Line; D002822:Choriocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004396:Coloring Agents; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002820:Hydatidiform Mole, Invasive / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D007124:Immunoenzyme Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D013778:Tetrazolium Salts; D013844:Thiazoles; D014162:Transfection; D014328:Trophoblastic Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins; D014594:Uterine Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology
D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D051017:Apoptosis Regulatory Proteins; D004396:Coloring Agents; C102885:DAB2 protein, human; D011506:Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D025521:Tumor Suppressor Proteins; C022616:thiazolyl blue