Antibody response to specific H. pylori antigens in functional dyspepsia, duodenal ulcer disease, and health.
OBJECTIVE
The relationship between H. pylori and functional dyspepsia remains controversial. The aim of this study was to identify a potential link between the antibody response to specific H. pylori antigens and functional dyspepsia.


METHODS
A total of 50 consecutive patients with functional dyspepsia, 50 patients with duodenal ulcer (DU), and 150 healthy blood donor control subjects with no history of peptic ulceration were studied. H. pylori status was determined by IgG antibodies using a validated ELISA. In H. pylori-positive subjects, antibodies against specific H. pylori antigens were identified by Western blot.


RESULTS
All DU patients (100%; 95%; CI, 93-100), 30 of 50 patients with functional dyspepsia (60%; 95% CI, 45-74) and 65 of 150 (43.3%; 95% CI, 34.3-51) blood donor controls tested positive for H. pylori. Forty-six of 50 (92%; 95% CI, 81-98) DU patients tested positive for the 91 kDa antigen (vacA) compared with 46 of 65 (69%; 95% CI, 58-81) control subjects and 22 of 30 (73%; 95% CI, 54-88) functional dyspepsia patients (p < 0.01 DU versus controls). Similarly, the 120 kDa antigen (cagA) tended (p < 0.15) to be more prevalent in DU patients (82%; 95% CI, 69-91) compared with controls (69%; 95% CI, 57-80) but not functional dyspepsia (77%; 95% CI, 57-90). No specific H. pylori antigens were associated with dyspepsia subgroups.


CONCLUSIONS
No specific H. pylori antigens are linked to functional dyspepsia.
Department of Gastroenterology, University of Essen, Germany.
G
G
Holtmann
N J
NJ
Talley
H
H
Mitchell
S
S
Hazell
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology*; D001782:Blood Donors; D015153:Blotting, Western / Q000379:methods; D016009:Chi-Square Distribution; D016001:Confidence Intervals; D004381:Duodenal Ulcer / Q000276:immunology*; D004415:Dyspepsia / Q000276:immunology*; D016481:Helicobacter Infections / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D011795:Surveys and Questionnaires
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial