Processing of free cells for electron microscopy using a fibrin clot.
A method of fibrin clot preembedding permitting the simple and gentle handling of free cells to be processed for electron microscopy is described. This technique is particularly useful for immunocytochemical techniques such as Lowicryl and thawed croysection approaches and represents a convenient alternative to procedures such as gelatine or agar preembeddings.
Department of Cell Biology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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Raska
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Pliss
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Mandys
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Risueno
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Lojda
D000911:Antibodies, Monoclonal; D020285:Cryoelectron Microscopy / Q000379:methods*; D004247:DNA / Q000032:analysis; D005337:Fibrin / Q000648:ultrastructure; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D016613:Plastic Embedding / Q000379:methods*
D000911:Antibodies, Monoclonal; D005337:Fibrin; D004247:DNA