Recombinant fowlpox viruses coexpressing chicken type I IFN and Newcastle disease virus HN and F genes: influence of IFN on protective efficacy and humoral responses of chickens following in ovo or post-hatch administration of recombinant viruses.
We have constructed recombinant (r) fowl pox viruses (FPVs) coexpressing chicken type I interferon (IFN) and/or hemagglutinin-neuraminidase (HN) and fusion (F) proteins of Newcastle disease virus (NDV). We administered rFPVs and FPV into embryonated chicken eggs at 17 days of embryonation or in chickens after hatch. Administration of FPV or rFPVs did not influence hatchability and survival of hatched chicks. In ovo or after hatch vaccination of chickens with the recombinant viruses resulted in protection against challenge with virulent FPV and NDV. Chickens vaccinated with FPV or FPV-NDV recombinant had significantly lower body weight 2 weeks following vaccination. This loss in body weight was not detected in chickens receiving FPV-IFN and FPV-NDV-IFN recombinants. Chickens vaccinated with FPV coexpressing IFN and NDV genes produced less antibodies against NDV in comparison with chickens vaccinated with FPV expressing NDV genes.
Department of Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108, USA.
K
K
Karaca
J M
JM
Sharma
B J
BJ
Winslow
D E
DE
Junker
S
S
Reddy
M
M
Cochran
J
J
McMillen
D000818:Animals; D000917:Antibody Formation; D001835:Body Weight; D002642:Chick Embryo; D002645:Chickens; D005586:Fowlpox / Q000517:prevention & control*; D005587:Fowlpox virus / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D015870:Gene Expression; D015707:HN Protein / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D007370:Interferon Type I / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology*; D009521:Newcastle Disease / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D014614:Vaccines, Synthetic / Q000235:genetics* / Q000627:therapeutic use*; D014760:Viral Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology
D015707:HN Protein; D007370:Interferon Type I; D014614:Vaccines, Synthetic; D014760:Viral Fusion Proteins