Two-step purification of mature human tumor necrosis factor alpha (rhTNF-alpha) protein produced using E. coli protein expression system.
Department of Pharmaceutical Sciences, Aristotle University of Thessaloniki, Greece.
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Kritis
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Tsiftsoglou
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins / Q000096:biosynthesis; D002851:Chromatography, High Pressure Liquid; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D015870:Gene Expression; D006801:Humans; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification*
D018528:ATP-Binding Cassette Transporters; D002352:Carrier Proteins; D018076:DNA, Complementary; D029968:Escherichia coli Proteins; D058619:Maltose-Binding Proteins; D009004:Monosaccharide Transport Proteins; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; C461599:maltose transport system, E coli