Crystal structure of piratoxin-I: a calcium-independent, myotoxic phospholipase A2-homologue from Bothrops pirajai venom.
The crystal structure of Piratoxin-I (PrTX-I) a Lys49 homologue isolated from the venom of Bothrops pirajai has been determined and refined at 2.8 A to a crystallographic residual of 19.7% (Rfree = 29.7%). Amino-acid sequence differences between catalytically active phospholipases and PrTX-I in the putative Ca2+-binding loop, specifically the substitutions Tyr28 --> Asn, Gly32 --> Leu and Asp49 --> Lys, result in an altered conformation of this loop. The analysis of the position of the epsilon-amino group of Lys49 in the PrTX-I structure indicates that it fills the site normally occupied by the calcium ion in the catalytically active phospholipases. In contrast to the homologous monomeric Lys49 variant from Agkistrodon piscivorus piscivorus (App), PrTX-I is present as a dimer in the crystalline state, as observed in the structures of myotoxin II from Bothrops asper and Bothropstoxin I from Bothrops jararacussu. The two molecules in the asymmetric unit in the crystal structure of PrTX-I are related by a nearly perfect two-fold symmetry axis, yet the dimeric structure is radically different from the dimeric structure of the phospholipase from Crotalus atrox. In the C. atrox structure the dimer interface occludes the active sites, whereas in the PrTX-I structure they are exposed to solvent.
Department of Physics, IBILCE/UNESP, São José do Rio Preto - SP, Brazil.
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de Azevedo
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Ward
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D000818:Animals; D017837:Bothrops; D002118:Calcium / Q000378:metabolism; D003435:Crotalid Venoms / Q000737:chemistry* / Q000633:toxicity; D018360:Crystallography, X-Ray; D054501:Group II Phospholipases A2; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000187:drug effects; D010741:Phospholipases A / Q000737:chemistry* / Q000633:toxicity; D054467:Phospholipases A2; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D030162:Reptilian Proteins
D003435:Crotalid Venoms; D030162:Reptilian Proteins; C114085:piratoxin I; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2; D002118:Calcium