Isolation of mutants deficient in acetyl-CoA synthetase and a possible regulator of acetate induction in Aspergillus niger.
Acetate-non-utilizing mutants in Aspergillus niger were selected by resistance to 1.2% propionate in the presence of 0.1% glucose. Mutants showing normal morphology fell into two complementation groups. One class of mutant lacked acetyl-CoA synthetase but had high levels of isocitrate lyase, while the second class showed reduced levels of both acetyl-CoA synthetase and isocitrate lyase compared to the wild-type strain. By analogy with mutants selected by resistance to 1.2% propionate in Aspergillus nidulans, the properties of the mutants in A. niger suggest that the mutations are either in the structural gene for acetyl-CoA synthetase (acuA) or in a possible regulatory gene of acetate induction (acuB). A third class of mutant in a different complementation group was obtained which had abnormal morphology (yellow mycelium and few conidia); the specific lesion in these mutants has not been determined.
Department of Biological Sciences, University of HullHull HU6 7RXUK.
Heather M
HM
Sealy-Lewis
Department of Biological Sciences, University of HullHull HU6 7RXUK.
Valerie
V
Fairhurst
D000106:Acetate-CoA Ligase / Q000172:deficiency* / Q000235:genetics; D001234:Aspergillus niger / Q000166:cytology / Q000201:enzymology* / Q000235:genetics*; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial; D005800:Genes, Fungal; D005809:Genes, Regulator; D007522:Isocitrate Lyase / Q000378:metabolism; D009154:Mutation; D011422:Propionates / Q000008:administration & dosage
D011422:Propionates; D007522:Isocitrate Lyase; D000106:Acetate-CoA Ligase