Immunocytochemical localization of a putative inhibitory amino acid receptor subunit in the parasitic nematodes Haemonchus contortus and Ascaris suum.
A rabbit antiserum was raised against a synthetic peptide corresponding to a region near the N-terminus of the Haemonchus contortus inhibitory amino acid receptor subunit, HG1. The antiserum recognized a recombinant form of the N-terminal domain of the subunit on Western blots and reacted with the ventral nerve cord of H. contortus in immunofluorescence experiments. Immunofluorescence was also detected in specific head neurons of H. contortus: these were tentatively identified as ring motor- and inter-neurons, plus a possible sensory neuron equivalent to the AQR cell of Caenorhabditis elegans. In the roundworm Ascaris suum, immunoreactivity was limited to the muscle arms, the post-synaptic component of the neuromuscular junction. The possible ligand of receptors containing the HG1 subunit is discussed in the light of this expression pattern.
Skinner
T M
TM
Department of Biology and Biochemistry, University of Bath, UK.
Bascal
Z A
ZA
Holden-Dye
L
L
Lunt
G G
GG
Wolstenholme
A J
AJ
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology; D017165:Ascaris suum / Q000737:chemistry* / Q000276:immunology; D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006189:Haemonchus / Q000737:chemistry* / Q000276:immunology; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000138:chemical synthesis / Q000276:immunology; D011817:Rabbits; D017459:Receptors, Amino Acid / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis
D000909:Antibodies, Helminth; D009479:Neuropeptides; D017459:Receptors, Amino Acid; D011993:Recombinant Fusion Proteins