Molecular temporal bone pathology: IV. Analysis of DNA template length using mitochondrial PCR primers.
The focus of this study was to identify, via molecular biology techniques, the length of the DNA templates present in individual archival celloidin-embedded human temporal bone sections. Earlier studies have suggested that the maximum template length present in these tissues is on the order of 471 base pair (bp). Polymerase chain reaction (PCR) amplification of 92 bp, 121 bp, 471 bp, and 609 bp regions of mitochondrial DNA (mtDNA), extracted from single archival celloidin-embedded human temporal bone sections, was used to assess the length of the template DNA extracted. These data are crucial to determine the limits of applying PCR technology to amplify specific genomic DNA targets located within the human inner ear. The results described should be of value to those investigators extracting DNA from archival individual human temporal bone sections for polymerase chain reaction assays of specific genetic alterations or infectious agents associated with temporal bone pathologies.
Wackym
P A
PA
Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee 53226, USA.
Kerner
M M
MM
Grody
W W
WW
D017931:DNA Primers / Q000235:genetics; D004261:DNA Replication; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013701:Temporal Bone; D016610:Tissue Embedding
D017931:DNA Primers; D004272:DNA, Mitochondrial