Detection of double-stranded RNA molecules and virus-like particles in different Mucor species.
The presence of double-stranded RNA elements was examined in 123 strains representing 18 Mucor species. These genetic elements were found to be present in 6 strains: 1 M. aligarensis, 1 M. hiemalis, 2 M. corticolus, 1 M. mucedo and 1 M. ramannianus. Electrophoretic separation of the nucleic acids revealed 4 different RNA patterns, with 1 to 5 discrete dsRNA bands. The molecular weights corresponding to these bands were 1.42-4.15 x 10(6) D. Using electronmicroscopy, for the first time the presence of virus like particles in Mucor species has been revealed.
Department of Microbiology, Attila József University, Szeged, Hungary.
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Vágvölgyi
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Magyar
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Papp
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Vastag
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Ferenczy
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Hornok
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Fekete
D004587:Electrophoresis, Agar Gel; D008854:Microscopy, Electron; D009089:Mucor / Q000235:genetics* / Q000302:isolation & purification / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure*; D012330:RNA, Double-Stranded / Q000235:genetics / Q000302:isolation & purification*
D012330:RNA, Double-Stranded