Epidemiological survey on canine population with the use of immunoleish skin test in endemic areas of human American cutaneous leishmaniasis in the state of Rio de Janeiro, Brazil.
A survey for canine tegumentary leishmaniasis (CTL) has been carried out between 1986 and 1993 in seven endemic localities for American cutaneous leishmaniasis in the State of Rio de Janeiro. 270 dogs have been examined for their clinical aspects, the development of delayed hypersensitivity (DHS) with Immunoleish antigen and with immunofluorescent antibody research of IgG (IF). 28.2% of them had ulcer lesions and 3.3% had scars. The lesions consisted of single (39.5%) and mucocutaneous lesions (31.6%), multiple cutaneous (25.0%) and mucocutaneous lesions associated with cutaneous ulcers (4.0%). Twelve (15.8%) isolates from biopsies were analyzed by zimodeme and schizodeme and identified as L. (V) braziliensis. The overall prevalence of canine infection that was evaluated with the skin test was of 40.5% and with IF it was of 25.5%. Both tests showed a high positive rate with relation to the animals with mucosal lesions, as in the case of human mucocutaneous leishmaniasis. The comparison of the two tests showed the skin test to have a better performance although there was no statistical difference (p > 0.05) between them. The proportional sensitivity and specificity was of 84.0% and 74.0%, respectively. The Immunoleish skin test and IF are useful tools to be employed in CTL field epidemiological surveys.
National School of Public Health, FIOCRUZ, Rio de Janeiro, Brazil. ebsantos@manguinhos.fiocruz.ensp.br
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EG
Santos
M C
MC
Marzochi
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NF
Conceição
C M
CM
Brito
R S
RS
Pacheco
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D016009:Chi-Square Distribution; D004283:Dog Diseases / Q000175:diagnosis* / Q000453:epidemiology*; D004285:Dogs; D019353:Endemic Diseases / Q000662:veterinary; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006306:Health Surveys; D016773:Leishmaniasis, Cutaneous / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D008297:Male; D015995:Prevalence; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D012882:Skin Tests / Q000379:methods