Identification of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) by using polymerase chain reaction amplification and restriction analysis of the mitochondrial cytochrome b gene.
Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the identification of fresh and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Digestion of the 359-bp PCR product with the endonucleases EcoRV and TaqI yielded specific banding patterns for salmon and trout. This genetic marker can be very useful for detecting fraudulent substitution of the cheaper smoked trout for the more expensive smoked salmon.
Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
E
E
Carrera
T
T
García
A
A
Céspedes
I
I
González
B
B
Sanz
P E
PE
Hernández
R
R
Martín
D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics*; D005819:Genetic Markers; D017686:Oncorhynchus mykiss / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping / Q000379:methods; D012474:Salmon / Q000145:classification / Q000235:genetics*
D003573:Cytochrome b Group; D005819:Genetic Markers