Novel diagnostic and therapeutic strategies with genetically engineered human antibodies.
Human antibodies can be generated by recombinant technology. The human immunoglobulin repertoire can be tapped in an effective manner by the so called V gene phage display technology. Using this technique the genes encoding the variable domains of an antibody of interest can be captured from the B cell repertoire. With these V genes tailor-made immune recognition molecules can be obtained by a 'design and build' strategy. These novel developments will lead to the introduction of a manifold of antibody based therapies into the clinic in coming decades. Recombinant antibody technology also provides unique opportunities to study the molecular structure of antibody variable domains against blood cell antigens. Such studies might possibly lead to the development of new therapies for antibody mediated blood cell destruction.
Department of Haematology, University of Cambridge, UK. who1000@cam.ac.uk
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Ouwehand
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Watkins
D000906:Antibodies / Q000235:genetics / Q000627:therapeutic use*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000627:therapeutic use; D000916:Antibody Diversity; D016824:Antigens, Human Platelet / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology; D003429:Cross Reactions; D015322:Gene Rearrangement, B-Lymphocyte; D005803:Genes, Immunoglobulin; D005822:Genetic Vectors; D006801:Humans; D007116:Immunization, Passive; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000302:isolation & purification; D039661:Integrin beta3; D007518:Isoantibodies / Q000235:genetics; D015202:Protein Engineering / Q000379:methods; D011693:Purpura / Q000209:etiology / Q000276:immunology / Q000628:therapy; D016719:Radioimmunodetection; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000627:therapeutic use*; D018029:Rho(D) Immune Globulin; D065227:Transfusion Reaction
D000906:Antibodies; D000911:Antibodies, Monoclonal; D016824:Antigens, Human Platelet; C061248:ITGB3 protein, human; D007135:Immunoglobulin Variable Region; D039661:Integrin beta3; D007518:Isoantibodies; C061961:RHO(D) antibody; D011993:Recombinant Fusion Proteins; D018029:Rho(D) Immune Globulin