Structure, expression and phylogenetic analysis of the glycoprotein gene of Cocal virus.
A cDNA copy of the mRNA of the glycoprotein G of Cocal virus, a rhabdovirus, has been cloned, sequenced and expressed in mammalian cells. The deduced amino acid sequence shows a typical transmembrane glycoprotein, 512 amino acids in length, containing two potential N-linked glycosylation sites. The amino acid sequence showed a high degree of identity with that of the prototype vesicular stomatitis virus serotype Indiana [VSV (IND)] G protein. In addition, phylogenetic analysis of amino acid sequence differences among the G proteins of vesiculoviruses indicated that Cocal virus represents a distinct lineage within the VSV (IND) serotype. Expression of the cloned Cocal G gene in mammalian cells produced a glycoprotein of mol.wt 71000 which was not palmitylated but induced cell fusion at acid pH.
Bhella
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RS
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Nichol
S T
ST
Wanas
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E
Ghosh
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HP
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D004279:DNA, Viral; D005814:Genes, Viral; D006023:Glycoproteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D007739:L Cells; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012209:Rhabdoviridae / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D014764:Viral Proteins; C114950:glycoprotein G, Cocal virus