Determination of eight trichothecenes by gas chromatography-mass spectrometry after sample clean-up by a two-stage solid-phase extraction.
A method is described for the determination of eight trichothecenes of type A and B in a variety of complex matrices including heavily moulded and pigmented cereals, whole cereal ears, cereal-based foods, mixed feeds and faeces from swine. Trichothecenes were determined as their trifluoroacetyl derivatives by gas chromatography with ion-trap mass spectrometry detection operating in chemical ionization mode. Isobutane was chosen as reactant gas and optimum parameters of measurement were determined. For sample preparation a clean-up procedure was developed using a combination of Florisil and cation-exchange cartridges for solid-phase extraction. Limits of detection and quantification as well as recoveries are described.
Department of Animal Nutrition, University of Hohenheim, Stuttgart, Germany.
M
M
Schollenberger
U
U
Lauber
H T
HT
Jara
S
S
Suchy
W
W
Drochner
H M
HM
Müller
D000818:Animals; D001939:Bread / Q000032:analysis; D005243:Feces / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007202:Indicators and Reagents; D009183:Mycotoxins / Q000032:analysis*; D012275:Oryza / Q000737:chemistry; D013552:Swine; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry
D007202:Indicators and Reagents; D009183:Mycotoxins; D014255:Trichothecenes