[The effect of ascorbic acid (vitamin C) on the proliferation of human lymphocytes stimulated with phytohemagglutinin (PHA)].
The lymphocytes (in whole blood) from 15 human patients were stimulated with phytohaemagglutinin (PHA)--40 micrograms/ml in standard 199 medium supplemented with ascorbic acid (AsA). As against the standard medium, the lymphocyte proliferation in AsA-added medium represented, on the average, 183 and 330% with the 40 and 80 micrograms/ml doses respectively. No mitotic activity (SI approximately 1) was recorded with the 160 micrograms/ml dose (two samples). When no PHA was present, the AsA addition did not induce lymphocyte proliferation. The amplification of the lymphocytic mitogenesis, as an AsA effect, was present irrespective of the SI value of the variants stimulated with PHA in standard medium, without exceeding 500%.
Institutul Naţional de Medicină Veterinară Pasteur, Bucureşti.
M
M
Samarineanu
D000328:Adult; D001205:Ascorbic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology*; D012621:Seasons; D013268:Stimulation, Chemical
D010835:Phytohemagglutinins; D001205:Ascorbic Acid