Spontaneous shift of an HSV type 1 productive, persistently infected macrophage-like culture to a non-productive culture.
A herpes simplex virus type 1 (HSV1) productive, persistently infected murine macrophage-like culture was obtained by infection at low multiplicity (0.001). Persistent cultures were characterized by spontaneously ceasing to produce infectious viruses after initial HSV1 production. A similar pattern of virus production was observed in cultures obtained by reinfection of the persistent macrophages with the original virus. Nonetheless, in persistent and reinfected cultures (17/18) which did not produce infectious viruses, viral proteins were detected by immunoblot, although their molecular weight, number and pattern differed among the cultures.
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF.
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Tirado
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Sarmiento
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B
Gómez
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002522:Chlorocebus aethiops; D018259:Herpesvirus 1, Human / Q000502:physiology*; D008264:Macrophages / Q000821:virology*; D051379:Mice; D014709:Vero Cells; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D014764:Viral Proteins