Separation and purification of 10-deacetylbaccatin III by high-speed counter-current chromatography.
High-speed counter-current chromatography (HSCCC) was used for the separation and purification of 10-deacetylbaccatin III from the needle extract of Chinese yew (Taxus chinensis). The crude needle extract (500 mg/5 ml) was first separated with a two-phase solvent system composed of n-hexane-ethyl acetate-ethanol-water (2:5:2:5, v/v) and the partially purified fraction was again purified with a different solvent system composed of n-hexane-chloroform-methanol-water (5:25:34:20, v/v). HPLC analysis of the final fraction showed that the purity of 10-deacetylbaccatin (20 mg) was over 98%. The chemical structure was confirmed by fast atom bombardment MS and 1H NMR.
Cao
X
X
Beijing Institute for New Technology Application, China.
Tian
Y
Y
Zhang
T Y
TY
Ito
Y
Y
D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D010936:Plant Extracts / Q000032:analysis; D010946:Plants, Medicinal / Q000737:chemistry; D012997:Solvents; D016339:Spectrometry, Mass, Fast Atom Bombardment; D043823:Taxoids; D014315:Triterpenes / Q000302:isolation & purification*
D007202:Indicators and Reagents; D010936:Plant Extracts; D012997:Solvents; D043823:Taxoids; D014315:Triterpenes; C042213:10-deacetylbaccatine III