Dominant expression of rat prostanoid DP receptor mRNA in leptomeninges, inner segments of photoreceptor cells, iris epithelium, and ciliary processes.
Prostaglandin (PG) D2 is one of the major prostanoids in the mammalian brain and eye tissues. Its function is mediated by the prostanoid DP receptor, which is specific for PGD2 among the various prostanoids. In this study, we cloned the full-length cDNA for the rat DP receptor and used it for detection of DP receptor mRNA in various rat tissues. Northern blotting and RT-PCR analyses revealed that this DP receptor was expressed most intensely in the eye tissues, moderately in the leptomeninges and oviduct, and weakly in the epididymis. The tissue distribution profile of the mRNA for the rat DP receptor is overlapped with those of hematopoietic and lipocalin-type PGD synthases. Among rat eye tissues, the expression was the highest in the iris. In situ hybridization and in situ RT-PCR revealed DP receptor mRNA to be localized in the epithelium of the iris and ciliary body and in photoreceptor cells of the retina, suggesting the involvement of the receptor in the physiological regulation of intraocular pressure and the vision process. In the brain, DP receptor mRNA was dominantly expressed in the leptomeninges and was not detected in the brain parenchyma including the ventral rostral forebrain, the surface area of which is reportedly involved in sleep induction by PGD2.
Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Suita, Japan.
D
D
Gerashchenko
C T
CT
Beuckmann
Y
Y
Kanaoka
N
N
Eguchi
W C
WC
Gordon
Y
Y
Urade
N G
NG
Bazan
O
O
Hayaishi
D000595:Amino Acid Sequence; D000818:Animals; D001099:Arachnoid / Q000378:metabolism*; D002924:Ciliary Body / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005123:Eye / Q000378:metabolism*; D005260:Female; D005799:Genes, Dominant / Q000235:genetics; D007498:Iris / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D010786:Photoreceptor Cells / Q000378:metabolism; D010841:Pia Mater / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D011971:Receptors, Immunologic; D011982:Receptors, Prostaglandin; C036293:prostaglandin D2 receptor