Inhibitory effects of citrus fruits on the mutagenicity of 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid treated with nitrite in the presence of ethanol.
It has been shown that the mutagenicity of 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid (MTCCA), a mutagen precursor in soy sauce treated with nitrite, was strongly increased when it was treated with nitrite in the presence of alcohols. We found that the mutagenicity of MTCCA treated with 50 mM nitrite at pH 3, 37 degrees C for 60 min in the presence of 7.5% ethanol was reduced by the addition of citrus fruits sudachi (Citrus sudachi), lemon (C. limon) and yuzu (C. junos), to the reaction mixture. The mutagenicity-reducing activity per weight of flavedos (outer colored portions of peel) of the citrus fruits was considerably higher than that of the juices. The juices of the other citrus fruits commercially available in Japan also had mutagenicity-reducing activity against the nitrite-treated MTCCA. Among the many components of citrus fruits, dietary fibers lignin and pectin showed strong antimutagenic activity in the reaction mixture, suggesting that the mixed fractions of these components including lignin, pectin, D-limonene, naringin, hesperidin, ascorbic acid and citric acid reduce the mutagenicity of MTCCA in the reaction mixture containing nitrite and ethanol.
Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Japan.
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M
Higashimoto
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H
Yamato
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T
Kinouchi
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Y
Ohnishi
D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry*; D002243:Carbolines / Q000737:chemistry* / Q000633:toxicity*; D002957:Citrus / Q000737:chemistry*; D003212:Condiments / Q000633:toxicity; D000431:Ethanol / Q000737:chemistry*; D066298:In Vitro Techniques; D009153:Mutagens / Q000737:chemistry* / Q000633:toxicity*; D009573:Nitrites / Q000737:chemistry*; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013025:Glycine max / Q000737:chemistry; D014439:Tyramine / Q000737:chemistry / Q000633:toxicity
D016587:Antimutagenic Agents; D002243:Carbolines; D009153:Mutagens; D009573:Nitrites; D000431:Ethanol; C052025:1,2,3,4-tetrahydro-1-methyl-beta-carboline-1-carboxylic acid; D014439:Tyramine