Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication.
The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivity. On the contrary, the thiol-alkylating agent 5,5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups, and certain sugar moieties of viral glycoproteins play an important role in the first steps of JV infection. Also enzymatic treatment of cell monolayers and addition of concanavalin A to cultures prior to infection suggest that cellular glycoproteins are involved in virus attachment.
Laboratorio de Virologia, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
L J
LJ
Raiger Iustman
V
V
Castilla
V
V
Meich
S E
SE
Mersich
D000477:Alkylating Agents / Q000494:pharmacology*; D000818:Animals; D002522:Chlorocebus aethiops; D003208:Concanavalin A / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005361:Ficain / Q000494:pharmacology; D018051:Junin virus / Q000187:drug effects* / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000502:physiology; D008623:Mercaptoethanol / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D010504:Periodic Acid / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D011991:Receptors, Virus / Q000187:drug effects / Q000502:physiology; D019163:Reducing Agents / Q000494:pharmacology*; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D010738:Type C Phospholipases / Q000494:pharmacology; D014709:Vero Cells; D014774:Virulence / Q000187:drug effects / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*
D000477:Alkylating Agents; D008562:Membrane Glycoproteins; D016877:Oxidants; D017680:Potassium Compounds; D011991:Receptors, Virus; D019163:Reducing Agents; D013439:Sulfhydryl Reagents; D010504:Periodic Acid; D003208:Concanavalin A; D008623:Mercaptoethanol; D004228:Dithionitrobenzoic Acid; D010738:Type C Phospholipases; D005361:Ficain; C037192:potassium periodate; D004229:Dithiothreitol