Experimental evaluation of hepatic functional reserve using 111In colloid for clinical application.
We have postulated that reduced hepatic blood flow is the primary factor responsible for postoperative hepatic insufficiency, and have shown the usefulness of the hepatic blood flow index (KL) estimated by 198Au colloid. However, 198Au colloid has became unavailable due to its intense exposure activity. Therefore, we prepared 111In colloid as a substitute for 198Au colloid to evaluate hepatic functional reserve. In the present study, a 111In colloid was administered to rats to investigate the possibility of clinical application. First, liver cirrhosis was induced in rats using CCl4 Changes in KL and hepatic tissue blood flow were measured by hydrogen gas clearance (HL) after CCl4 injection, and was shown to correlates well with it. The correlation between KL and HL, together with normal and cirrhotic liver, was significant (r = 0.83, p < 0.01) Second, reticuloendothelial function was modulated with Zymosan A (stimulation) and Silica (suppression). The KL value remained unchanged in spite of the addition of an activator or suppressor to the reticuloendothelial system. Third, 30% and 70% hepatectomy models were studied according to the method of Higgins-Anderson. KL and HL was measured before and 1, 3 and 7 days after hepatectomy. KL and HL after 30% and 70% hepatectomy showed similar changes. These results indicate that the clearance of 111In colloid can be used clinically as an index of the hepatic blood flow.
Sugiu
T
T
First Department of Surgery, Okayama University Medical School, Japan.
Hamazaki
K
K
Sakumoto
S
S
Mimura
H
H
Tanaka
N
N
D000818:Animals; D003102:Colloids; D005069:Evaluation Studies as Topic; D006498:Hepatectomy; D007204:Indium; D008099:Liver / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D008106:Liver Cirrhosis, Experimental / Q000000981:diagnostic imaging / Q000503:physiopathology*; D008297:Male; D012157:Mononuclear Phagocyte System / Q000098:blood supply; D011877:Radionuclide Imaging; D051381:Rats; D017208:Rats, Wistar
D003102:Colloids; D007204:Indium