Deciliation: A stressful event for Paracentrotus lividus embryos.
In this report, by using mono- and two-dimensional electrophoretic analysis, we demonstrate that deciliation on sea urchin embryos induces a stress response. Deciliation indeed causes not only the activation of ciliary subroutine, but also a transient decrease of bulk protein synthesis. This decrease is in agreement with our previous results on heat shock response in sea urchin, although deciliation does not induce the expression of the same main hsp set. We were able to characterize one main deciliation-stress protein of 40 kDa whose expression is transiently induced by deciliation and whose localisation is likely to be nuclear.
Casano
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C
Dipartimento di Biologia Cellulare e dello Sviluppo A. Monroy, Università di Palermo, Italy.
Roccheri
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MC
Onorato
K
K
Cascino
D
D
Gianguzza
F
F
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D002923:Cilia / Q000502:physiology*; D003593:Cytoplasm / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian / Q000502:physiology* / Q000648:ultrastructure; D005775:Gastrula / Q000502:physiology* / Q000648:ultrastructure; D008715:Methionine / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification; D012038:Regeneration; D012462:Saline Solution, Hypertonic; D012617:Sea Urchins / Q000196:embryology*; D013312:Stress, Physiological
D011506:Proteins; D012462:Saline Solution, Hypertonic; D008715:Methionine