Two-dimensional 220 MHz Fourier transform EPR imaging.
In the last decade radiofrequency continuous-wave EPR spectrometers have been developed to detect and localize free radicals in vivo. Only recently, pulsed radiofrequency EPR spectrometers have been described for imaging applications with small samples. In the present work, we show the first two-dimensional image obtained at 220 MHz on a large phantom (40 ml) that simulates typical conditions of in vivo EPR imaging. This pulsed EPR apparatus has the potential to make the time required for three-dimensional imaging compatible with the biological half-life of normally used paramagnetic probes.
INFM and Dipartimento di Scienze e Tecnologie Biomediche, Università dell'Aquila, Italy. placidi@fismedw2.univaq.it
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D000818:Animals; D055592:Biophysical Phenomena; D001703:Biophysics; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods* / Q000706:statistics & numerical data; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D019047:Phantoms, Imaging; D011846:Radio Waves; D051381:Rats