Comparative molecular characterization of Corynebacterium pseudotuberculosis of different origin.
Ribotyping and susceptibility to 17 antimicrobial agents were used to compare 37 isolates of Corynebacterium pseudotuberculosis (28 from horses, 1 from cattle, 3 from sheep and 5 from goats) derived from various types of lesions, and different geographic locations. According to the presence of nitrate reductase, all but one isolate from horses reduced nitrate (nitrate-positive), whereas all isolates from sheep and goats were unable to reduce nitrate (nitrate-negative). The ribotype of the nitrate-negative isolate from a horse with ulcerative lymphangitis was identical to all the other isolates from horses, and different than the ribotype of nitrate-negative isolates from sheep and goats. Ribotyping with one of the restriction endonucleases, Apa 1, revealed differences between, but not within, the two biotypes. However, ribotyping with Pst 1 endonuclease revealed one variant within the equine biotype and one variant within the ovine biotype. The minimum inhibitory concentration (MIC; microgram/ml) of antimicrobial agents against isolates from nitrate-negative and nitrate-positive groups was very similar, with the exception of isolates from sheep and goats which had a higher MIC for amikacin than isolates from horses and cattle.
Costa
L R
LR
Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis 95616, USA. costa_l@vt8200.vetmed.lsu.edu
Spier
S J
SJ
Hirsh
D C
DC
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015139:Blotting, Southern / Q000662:veterinary; D002140:California; D002417:Cattle; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000145:classification* / Q000187:drug effects / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D006041:Goats; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D009516:New Mexico; D009566:Nitrates / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015183:Restriction Mapping / Q000662:veterinary; D012756:Sheep; D013019:South Africa
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009566:Nitrates; D012336:RNA, Ribosomal, 16S