Chemical and spectroscopic characterisation of the phosphatidylinositol manno-oligosaccharides from Mycobacterium bovis AN5 and WAg201 and Mycobacterium smegmatis mc2 155.
Two classes of phosphatidylinositol manno-oligosaccharides (PIMs) were isolated from each of Mycobacterium bovis AN5 and WAg201 and Mycobacterium smegmatis mc2 155. The deacylated PIMs (dPIMs), were identified as hexasaccharide (dPIM-6) and disaccharide (dPIM-2) species composed of mannose, myo-inositol, glycerol and phosphate residues in the proportions of 6:1:1:1 and 2:1:1:1, respectively. Structural analysis, employing a combination of microanalytical methods, nuclear magnetic resonance spectroscopy, and mass spectrometric techniques established that the sequence of residues within dPIM-6, as shown below, was identical in the three mycobacterial strains investigated.
Ag Research, Wallaceville Animal Research Centre, Upper Hutt, New Zealand. severnw@agresearch.cri.nz
W B
WB
Severn
R H
RH
Furneaux
R
R
Falshaw
P H
PH
Atkinson
D000942:Antigens, Bacterial / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002247:Carbon Isotopes; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000737:chemistry*; D009163:Mycobacterium bovis / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D010716:Phosphatidylinositols / Q000737:chemistry*; D017421:Sequence Analysis
D000942:Antigens, Bacterial; D002247:Carbon Isotopes; D009844:Oligosaccharides; D010716:Phosphatidylinositols; C034154:phosphatidylinositol mannoside