Hepatoprotective effect of C-phycocyanin: protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats.
Effect of C-phycocyanin (from Spirulina platensis) pretreatment on carbontetrachloride and R-(+)-pulegone-induced hepatotoxicity in rats was studied. Intraperitoneal (i.p.) administration (200 mg/kg) of a single dose of phycocyanin to rats, one or three hours prior to R-(+)-pulegone (250 mg/kg) or carbontetrachloride (0.6 ml/kg) challenge, significantly reduced the hepatotoxicity caused by these chemicals. For instance, serum glutamate pyruvate transaminase (SGPT) activity was almost equal to control values. The losses of microsomal cytochrome P450, glucose-6-phosphatase and aminopyrine-N-demethylase were significantly reduced, suggesting that phycocyanin provides protection to liver enzymes. It was noticed that the level of menthofuran, the proximate toxin of R-(+)-pulegone was nearly 70% more in the urine samples collected from rats treated with R-(+)-pulegone alone than rats treated with the combination of phycocyanin and R-(+)-pulegone. The possible mechanism involved in the hepatoprotection is discussed.
Vadiraja
B B
BB
Department of Organic Chemistry, Indian Institute of Science, Bangalore, 560 012, India.
Gaikwad
N W
NW
Madyastha
K M
KM
D000410:Alanine Transaminase / Q000097:blood; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury; D000081005:Cyclohexane Monoterpenes; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology / Q000517:prevention & control*; D008297:Male; D008610:Menthol / Q000031:analogs & derivatives / Q000633:toxicity / Q000652:urine; D008862:Microsomes, Liver / Q000201:enzymology; D039821:Monoterpenes; D010798:Phycocyanin / Q000627:therapeutic use*; D051381:Rats; D013729:Terpenes / Q000652:urine
D000081005:Cyclohexane Monoterpenes; D039821:Monoterpenes; D013729:Terpenes; D010798:Phycocyanin; D008610:Menthol; C054487:menthofuran; C039648:pulegone; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D000633:Aminopyrine N-Demethylase; D000410:Alanine Transaminase; D005952:Glucose-6-Phosphatase