A novel class of mosquitocidal delta-endotoxin, Cry19B, encoded by a Bacillus thuringiensis serovar higo gene.
Partially digested HincII fragments of DNA from a mosquitocidal strain of Bacillus thuringiensis serovar higo were cloned into pBluescript II SK(+) and propagated in Escherichia coli. Recombinant cells were screened immunologically for the production of parasporal inclusion antigens. One E. coli clone harboring a recombinant plasmid exhibited larvicidal activity to Culex pipiens molestus, but not to Anopheles stephensi. Hybridization experiments revealed that the gene of the toxin protein is located on a 110 kb plasmid of B. thuringiensis serovar higo. Sequence analysis detected an open reading frame of 2046 nucleotides encoding a polypeptide of 682 amino acid residues with a predicted molecular weight of 78,467. The gene encoded five block regions commonly conserved in the insecticidal protein genes of B. thuringiensis. Amino acid sequence of the 78 kDa protein shared 49% identity and 56% similarity with that of the Cry19A protein from B. thuringiensis serovar jegathesan. A new class of delta-endotoxin protein, designated Cry19B, was established on the basis of this protein.
Section of Applied Microbiology, Fukuoka Industrial Technology Centre, Japan.
S H
SH
Hwang
H
H
Saitoh
E
E
Mizuki
K
K
Higuchi
M
M
Ohba
D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles; D001413:Bacillus thuringiensis / Q000737:chemistry / Q000235:genetics*; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D017124:Conserved Sequence; D003465:Culex; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial; D006041:Goats; D006460:Hemolysin Proteins; D007306:Insecticides; D008969:Molecular Sequence Data; D009032:Mosquito Control; D010572:Pest Control, Biological; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis