Evaluation of the RapID CB plus system for identification of coryneform bacteria and Listeria spp.
In a stress test, the recently introduced RapID CB Plus system (Remel Inc. [formerly Innovative Diagnostic Systems], Norcross, Ga.) was challenged with a diverse set of gram-positive rods comprising 345 strains of coryneform bacteria and 33 strains of Listeria spp. representing a total of 49 different taxa. Overall, within 4 h, the system correctly identified 80.9% of the strains on the species level and 12.2% of the strains on the genus level. Only 3.7% strains were misidentified, and for 3.2% of the strains no identification was provided. Difficulties with the system were mainly due to occasional uncertainties in reading reactions for acid production from carbohydrates and, to a lesser extent, aminopeptidase reactions. It is concluded that the system may also perform well under the conditions of a routine clinical laboratory.
Department of Medical Microbiology, University of Zürich, CH-8028 Zürich, Switzerland.
G
G
Funke
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K
Peters
M
M
Aravena-Roman
D000192:Actinomycetales / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D000626:Aminopeptidases; D050260:Carbohydrate Metabolism; D005188:False Negative Reactions; D005189:False Positive Reactions; D016987:Gram-Positive Rods / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D007202:Indicators and Reagents; D008087:Listeria / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification; D009391:Nephelometry and Turbidimetry / Q000379:methods; D015203:Reproducibility of Results; D012984:Software
D007202:Indicators and Reagents; D000626:Aminopeptidases