Analysis of HLA class II and TAP alleles in Japanese patients with psoriasis vulgaris.
We investigated HLA class II and transporter associated with antigen processing (TAP) alleles in eighty-five unrelated Japanese patients with psoriasis vulgaris and fifty-two healthy controls using the polymerase chain reaction-restriction fragment length polymorphism method. The frequencies of DRB1*1502 and DQB1*0601 were increased in the patient group (DRB1*1502; 21% vs 12%, p < 0.05, DQB1*0601; 35% vs. 21%, p < 0.05), while the frequencies of DRB1*0406 and TAP2*E were decreased in the patients (DRB1*0406; 2% vs 9%, p < 0.05, TAP2*E; 4% vs 11%, p < 0.05). However, none of these remained significant after p values were corrected for the number of comparisons made (pc > 0.05). We also analysed specific amino acids on HLA class II molecules, but no significant difference was found between the two groups. Our previous reports clarified that aspartate at residue 9 (48% vs 20%, p < 0.002) and alanine at residue 73 (81% vs 48%, p < 0.0001) on HLA-C molecules were strongly associated with Japanese patients with PsV. These specific amino acids on HLA-C molecules are supposed to play more important roles compared with HLA class II and TAP alleles in the development of psoriasis vulgaris.
Saeki
H
H
Department of Dermatology, Faculty of Medicine, University of Tokyo, Japan.
Kuwata
S
S
Nakagawa
H
H
Asahina
A
A
Tamaki
K
K
Shibata
Y
Y
Juji
T
T
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D000949:Histocompatibility Antigens Class II / Q000235:genetics*; D006801:Humans; D007564:Japan; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011565:Psoriasis / Q000235:genetics*
D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D017931:DNA Primers; D000949:Histocompatibility Antigens Class II; C071692:TAP2 protein, human; D004247:DNA