Adjuvant effects and antiserum action potentiation by a (herbal) compound 2-hydroxy-4-methoxy benzoic acid isolated from the root extract of the Indian medicinal plant 'sarsaparilla' (Hemidesmus indicus R. Br.).
The adjuvant effect and antiserum potentiation of a compound 2-hydroxy-4-methoxy benzoic acid were explored in the present investigation. This compound, isolated and purified from the Indian medicinal plant Hemidesmus indicus R. Br, possessed antisnake venom activity. Rabbits immunized with Vipera russellii venom in the presence and absence of the compound along with Freund's complete adjuvant, produced a precipitating band in immunogel diffusion and immunogel electrophoresis. The venom neutralizing capacity of this antiserum showed positive adjuvant effects as evident by the higher neutralization capacity (lethal and hemorrhage) when compared with the antiserum raised with venom alone. The pure compound potentiated the lethal action neutralization of venom by commercial equine polyvalent snake venom antiserum in experimental models. These observations raised the possibility of the use of chemical antagonists (from herbs) against snake bite, which may provide a better protection in presence of antiserum, especially in the rural parts of India.
Alam
M I
MI
Department of Physiology, University of Calcutta, India.
Gomes
A
A
D000276:Adjuvants, Immunologic / Q000627:therapeutic use*; D000818:Animals; D000997:Antivenins / Q000627:therapeutic use*; D001565:Benzoates / Q000302:isolation & purification / Q000627:therapeutic use*; D004357:Drug Synergism; D005779:Immunodiffusion; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D017840:Daboia / Q000276:immunology; D012909:Snake Bites / Q000276:immunology / Q000628:therapy*; D014757:Viper Venoms / Q000276:immunology
C112536:2-hydroxy-4-methoxy benzoic acid; D000276:Adjuvants, Immunologic; D000997:Antivenins; D001565:Benzoates; D014757:Viper Venoms