Rat carnitine palmitoyltransferase Ibeta mRNA splicing isoforms.
Carnitine palmitoyltransferase I (CPT-I) catalyzes the rate-determining step in mitochondrial fatty acid beta-oxidation. The enzyme has two cognate structural genes (alpha and beta) that are differentially expressed in tissues. We show multiple mature mRNAs in rat heart derived from alternative splicing of CPT-Ibeta transcripts. Two novel messages are deleted for regions of the previously described mRNA that encode membrane-spanning and regulatory domains, suggesting that the cognate isozymes will exhibit unique kinetic characteristics.
Diabetes Unit and Medical Services, Massachusetts General Hospital, 149 Thirteenth Street, MGH East, CNY 149 8218, Charlestown, MA 02129, USA.
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D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment
D007527:Isoenzymes; D015347:RNA Probes; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase