pHaMDR-DHFR bicistronic expression system for mutational analysis of P-glycoprotein.
Division of Oncology, Hospital for Sick Children, Toronto, Ontario, Canada.
S
S
Zhang
Y
Y
Sugimoto
T
T
Shoshani
I
I
Pastan
M M
MM
Gottesman
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular / Q000379:methods; D004252:DNA Mutational Analysis / Q000379:methods; D017931:DNA Primers; D018432:Drug Resistance, Multiple; D005822:Genetic Vectors; D006801:Humans; D007624:KB Cells; D008727:Methotrexate / Q000633:toxicity; D016254:Mutagenesis, Insertional; D016297:Mutagenesis, Site-Directed; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000378:metabolism; D013762:Tetrahydrofolate Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D014162:Transfection / Q000379:methods
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate