Protein recovery using gas-liquid dispersions.
Two separation techniques, foam separation and colloidal gas aphrons (CGAs), both of which are based on gas-liquid dispersions, are compared as potential applications for protein recovery in downstream processing. The potential advantages of each method are described and the concentration and selectivity achieved with each method, for a range of proteins is discussed. The physical basis of foam separation is the preferential adsorption of surface active species at a gas-liquid interface, with surface inactive species remaining in bulk solution. When a solution containing surface active species is sparged with gas, a foam is produced at the surface: this foam can be collected, and upon collapse contains surface active species in a concentrated form. CGAs are microbubble dispersions (bubble diameters 10-100 microm) with high gas hold ups (>50%) and relatively high stability, which are formed by stirring a surfactant solution at speeds above a critical value (typically around 5000 rpm). It is expected that when proteins are brought into contact with aphrons, protein adsorbs to the surfactant through electrostatic and/or hydrophobic forces. The aphron phase can be separated easily from the bulk solution due to its buoyancy, thus allowing separation of protein in a concentrated form.
Noble
M
M
Biotechnology and Biochemical Engineering Group, The University of Reading, UK.
Brown
A
A
Jauregi
P
P
Kaul
A
A
Varley
J
J
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002845:Chromatography; D003102:Colloids; D005740:Gases; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000302:isolation & purification*; D013501:Surface-Active Agents
D003102:Colloids; D005740:Gases; D011506:Proteins; D013501:Surface-Active Agents