[Ocular tissue concentrations and results of filtration surgery after topical use of mitomycin C in rabbits].
We measured mitomycin C (MMC) concentrations in rabbit ocular tissues, using high-performance liquid chromatography, after the subconjunctival application of surgical sponges which had absorbed various doses (0.1, 0.2 or 0.4 mg) of MMC and with varying duration of application (1, 3, or 5 minutes). MMC concentrations at the administered site showed a significant correlation with the dose and duration of application. In multiple regression analysis the MMC concentration (microgram/g) in the conjunctiva was -6.73 + 67.4 x dose (mg) + 1.66 x time (minutes) (R-square 0.65); in the sclera, -1.85 + 38.2 x dose + 0.927 x time (R-square 0.63); and in the corena, -0.727 + 8.44 x dose (R-square 0.46). We also compared the results of filtering surgery on rabbits using 0.2 mg MMC intraoperative exposure of 1, 3, 5 minutes or 5-minute application of distilled water (control). The duration of maintained intraocular pressure reduction and filtering bleb was significantly longer in MMC-treated group (1 minute; 15 +/- 7.1, 3 minutes; 27 +/- 14.3, 5 minutes; 29 +/- 18.6 days, mean +/- standard deviation) than in the control group (7 +/- 3.7 days). Among the MMC-treated groups, good results were maintained significantly longer in the 3- or 5-minute application groups than in the 1-minute group. There was no difference between the 3-minute group and the 5-minute group.
Hara
T
T
Department of Ophthalmology, University of Tokyo School of Medicine, Japan.
Shirato
S
S
Suzuki
Y
Y
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005123:Eye / Q000378:metabolism*; D018463:Filtering Surgery; D016685:Mitomycin / Q000493:pharmacokinetics*; D011817:Rabbits; D012044:Regression Analysis; D013997:Time Factors
D000903:Antibiotics, Antineoplastic; D016685:Mitomycin