Comparative genomic hybridization with lissamine- and fluorescein-labeled nucleotides.
Biotin deoxyuridine 5'-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously used in CGH (lissamine-5-dUTP and fluorescein-N6dATP) and show that this direct label combination performs at least as well as the indirect biotin/digoxigenin pair.
Rosenberg
C
C
Department of Molecular Cell Biology, Leiden University Medical Center, The Netherlands. carla@ruly46.leidenuniv.nl
van Gijlswijk
R P
RP
Vos
C B
CB
Wiegant
J
J
Cornelisse
C J
CJ
Tanke
H J
HJ
Raap
A K
AK
D004247:DNA / Q000097:blood; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005260:Female; D019793:Fluorescein / Q000378:metabolism*; D015698:Genomic Library; D006801:Humans; D008214:Lymphocytes / Q000737:chemistry; D008297:Male; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000378:metabolism*; D012235:Rhodamines / Q000378:metabolism*
D004273:DNA, Neoplasm; D009711:Nucleotides; D012235:Rhodamines; C022027:lissamine rhodamine B; D004247:DNA; D019793:Fluorescein