PCR assay fails to detect molluscum contagiosum virus-related sequences in AIDS-related Kaposi's sarcoma.
Previous PCR-based studies have demonstrated the presence of various viral DNA or RNA sequences in Kaposi's sarcoma (KS) tissues. To date, only human herpesvirus 8 (HHV-8) DNA sequences are found consistently in KS. The putative role of this agent in KS pathogenesis remains, however, to be determined; HHV-8 could infect populations endemically and could be reactivated in patients with KS. A close association between AIDS-related KS and molluscum contagiosum occurrence was found and this study was conducted primarily to search for the presence of molluscum contagiosum virus DNA sequences in KS. Frozen KS samples were examined for the presence of both HHV-8 and molluscum contagiosum virus DNA sequences by PCR. Despite a high rate of co-infection, no molluscum contagiosum virus (MCV) DNA sequence could be found in the KS samples whereas HHV-8 was uniformly detected. These results suggest that the high prevalence of MCV in AIDS patients with KS relies on a mode of transmission common for HHV-8 and molluscum contagiosum virus rather than on a multiviral etiology of KS. They may also indicate a particular susceptibility of the host to viral reactivation. If this is so, the failure to detect MCV DNA sequences in KS tissues by PCR indicates that locally produced or released cyotokines are not involved in the latter process.
Simonart
T
T
Department of Dermatology, Erasme University Hospital, Brussels, Belgium.
Noël
J C
JC
Van Vooren
J P
JP
Hermans
P
P
Liesnard
C
C
Fayt
I
I
Gilot
P
P
Godfroid
E
E
Parent
D
D
D017088:AIDS-Related Opportunistic Infections / Q000821:virology*; D005347:Fibroblasts / Q000821:virology; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification; D006716:Homosexuality; D006801:Humans; D008977:Molluscum contagiosum virus / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012514:Sarcoma, Kaposi / Q000821:virology*; D012867:Skin / Q000821:virology