Determination of flumequine and 7-hydroxyflumequine in plasma of sheep by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of flumequine and its metabolite 7-hydroxyflumequine in sheep plasma was described. The two compounds were extracted from 100 microl of plasma by liquid-liquid extraction. Aliquots (100 microl) were injected onto the HPLC system and separated on a LiChrospher Select B column with an isocratic system. The compounds were detected by fluorimetric detection for concentrations below 500 microg/l and by UV detection for the concentrations exceeding 500 microg/l. The range of the validated concentrations were 50000 to 5 microg/l and 500 to 10 microg/l with mean recovery rates of 87+/-3% and 60+/-1% for flumequine and 7-hydroxyflumequine, respectively.
Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire des Médicaments Vétérinaires, Fougères, France.
J M
JM
Delmas
A M
AM
Chapel
P
P
Sanders
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones; D011807:Quinolizines / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012756:Sheep / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; C040690:7-hydroxyflumequine; C012976:flumequine