The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses.
DF-1 is a continuous cell line of chicken embryo fibroblasts. The cells are free of endogenous sequences related to avian sarcoma and leukosis viruses and have normal fibroblastic morphology. DF-1 cells support the replication of avian retroviruses; diverse oncogenes induce foci of oncogenic transformation on monolayers of DF-1, and avian leukosis viruses of envelope subgroups B, D, and C induce cell death and form plaques. The new cell line will greatly facilitate studies on oncogenic transformation and cell killing by avian viruses.
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California, 92037, USA.
M
M
Himly
D N
DN
Foster
I
I
Bottoli
J S
JS
Iacovoni
P K
PK
Vogt
D000818:Animals; D001354:Avian Leukosis Virus / Q000502:physiology*; D016923:Cell Death; D002460:Cell Line / Q000502:physiology* / Q000821:virology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D005347:Fibroblasts / Q000502:physiology* / Q000821:virology; D016758:Genes, jun / Q000502:physiology; D009857:Oncogenes / Q000502:physiology*; D014779:Virus Replication