Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997.
The ISOBM TD-6 Workshop is the first international workshop on monoclonal antibodies against the Sialyl Lewisa (SLea) antigen. Eight research groups participated in a blind study to characterize the epitope binding, relative affinity and performance in immunoradiometric assays, of a panel of 20 monoclonal antibodies. The antibodies were tested against a diverse panel of neoglycoconjugates, purified antigens and human serum pools from gastrointestinal malignancies. Epitope specificities were determined for the majority of antibodies in the panel. Cross-reactivity with related saccharide structures was noted in several antibodies. Overall, the results of the TD-6 Workshop show further development of SLea immunoassays may yield yet more specific assays for the detection and management of gastrointestinal and other malignancies.
Rye
P D
PD
Central Laboratory, Norwegian Radium Hospital, Montebello, Oslo, Norway.
Bovin
N V
NV
Vlasova
E V
EV
Molodyk
A A
AA
Baryshnikov
A
A
Kreutz
F T
FT
Garinther
W I
WI
Schultes
B C
BC
Noujaim
A A
AA
Madiyalakan
R
R
Magnani
J
J
Nilsson
O
O
Nilsson
K
K
Nustad
K
K
Norum
L
L
Bell
H
H
Cao
Y
Y
Suresh
M R
MR
Very
D L
DL
Freeman
J V
JV
Yeung
K K
KK
Hilgers
J
J
D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000032:analysis*; D018395:CA-19-9 Antigen / Q000276:immunology / Q000378:metabolism; D002240:Carbohydrate Sequence; D000939:Epitopes / Q000276:immunology; D005732:Gangliosides / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D005770:Gastrointestinal Neoplasms / Q000097:blood; D006801:Humans; D015592:Immunoradiometric Assay; D008969:Molecular Sequence Data
D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D000939:Epitopes; D005732:Gangliosides; C064564:sialyl Le(a) ganglioside