Study of the morphology of the cell walls of some strains of lactic acid bacteria and related species.
The objective of the present study was to find an explanation for the biological effect of the bacteria present in a biotherapeutic milk (Lactobacillus casei CRL 431 and Lactobacillus acidophilus CRL 730). The ability of bacterial cell walls to induce an immune response when introduced into an organism is well known. In this paper we specifically analyzed the morphology of these cell walls. Besides the two bacterial strains used in the fermented milk, two other lactic acid bacteria, belonging to another genus and unable to induce an immune system response, as well as a strain of Propionibacterium, of which the immune modulating capacity is known, were used in this work. We found a structural particularly in strains with immunostimulating capacity (L. casei CRL 431 and P. acidopropionici CRL 1198): molecules which protrude from the cell surface. In L. casei CRL 431 these molecules were identified as lectins because they are able to agglutinate yeast cells treated with glutaraldehyde and glycine. The structures protruding from P. acidipropionici CRL 1198 cells were teichoic acids. Teichoic acid and lectin-like structures can participate in adhesion to intestinal cells. The immunostimulation observed can be induced by the adhesion phenomenon.
Centro de Referencia para Lactobacilos (CERELA), Tucumán, Argentina. VMORATA@CERELA.EDU.AR
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Morata de Ambrosini
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Gonzalez
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de Ruiz Holgado
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Oliver
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000372:Agglutination Tests; D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology*; D001646:Bile / Q000382:microbiology; D002448:Cell Adhesion / Q000187:drug effects; D002473:Cell Wall / Q000276:immunology* / Q000648:ultrastructure; D007422:Intestines / Q000187:drug effects; D007779:Lactobacillus acidophilus / Q000276:immunology* / Q000648:ultrastructure; D007780:Lacticaseibacillus casei / Q000276:immunology* / Q000648:ultrastructure; D037102:Lectins; D008565:Membrane Proteins / Q000276:immunology; D008892:Milk / Q000382:microbiology; D011424:Propionibacterium / Q000276:immunology* / Q000648:ultrastructure; D013682:Teichoic Acids / Q000494:pharmacology
D000276:Adjuvants, Immunologic; D001426:Bacterial Proteins; D037102:Lectins; D008565:Membrane Proteins; D013682:Teichoic Acids