Preferential silver reaction of nucleolar regions adjacent to fibrillar centers in ring shaped nucleoli of leukemic lymphocytes.
Silver stained proteins (SSPs) characteristic for interphasic nucleolus organizer regions (NORs) associated with fibrillar centers (FCs) and adjacent nucleolar regions of ring shaped nucleoli in leukemic lymphocytes exhibit a different sensitivity to the mild acid extraction including that with HCl. Such extractions permit a preferential visualization of fibrillar centers adjacent regions (FCARs) which are believed to represent sites of the ribosomal RNA (rRNA) transcription. The resistance of SSPs in FCARs to the extraction with HCl seems to be due to their binding to other components present in these regions. The extractibility of SSPs with HCl was influenced by the fixatives used. The largest resistance of SSPs to the extraction with HCl was noted after fixation with glutaraldehyde. In contrast, the largest extractibility of these proteins was observed after fixation with unbuffered formaldehyde.
Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
K
K
Smetana
I
I
Jirásková
M
M
Sedlácková
R
R
Dvorák
M
M
Spátová
P
P
Hozák
D001402:B-Lymphocytes / Q000737:chemistry* / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000737:chemistry* / Q000648:ultrastructure; D006367:HeLa Cells / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D006851:Hydrochloric Acid / Q000737:chemistry; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D009687:Nuclear Proteins / Q000032:analysis*; D009697:Nucleolus Organizer Region / Q000737:chemistry* / Q000648:ultrastructure; D012834:Silver / Q000737:chemistry; D016622:Silver Staining / Q000379:methods; D016707:Tissue Fixation
D009687:Nuclear Proteins; D012834:Silver; D006851:Hydrochloric Acid