Influence of interleukin-1 receptor antagonist on [3H]serotonin and histamine release by rat basophilic leukemia-2H3 cells.
Mast cells located in connective tissues are a potent source of vasoactive and inflammatory mediators, such as cytokines. They accumulate in tissues in a wide variety of diseases where their function in most cases in unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) (from 25.2 to 29.9%), and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-IRA-treated and -untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT spontaneous release from RBLC cultures.
Immunology Division, University of Chieti, Italy.
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D000818:Animals; D001921:Brain / Q000201:enzymology; D002470:Cell Survival / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects*; D006640:Histidine Decarboxylase / Q000096:biosynthesis; D053590:Interleukin 1 Receptor Antagonist Protein; D015471:Leukemia, Basophilic, Acute; D008407:Mast Cells / Q000502:physiology; D051381:Rats; D017472:Receptors, Interleukin-1 / Q000037:antagonists & inhibitors; D011994:Recombinant Proteins / Q000494:pharmacology; D012701:Serotonin / Q000378:metabolism*; D012795:Sialoglycoproteins / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D053590:Interleukin 1 Receptor Antagonist Protein; D017472:Receptors, Interleukin-1; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D006640:Histidine Decarboxylase