An essential role for ovarian inhibin in pineal gland-mediated anestrus in Syrian hamsters.
When transferred from long photoperiod (LP) to short photoperiod (SP), female Syrian hamsters exhibit depressions of follicle stimulating hormone (FSH) and follicular development, cessation of ovulation, and marked ovarian interstitial tissue hyperplasia. Pinealectomy prevents these effects of SP. The object of this study was to determine the role of inhibin in the regulation of FSH during SP-induced anestrus. Adult LSH/SsLak hamsters maintained in LP (LD 14:10 hr) were transferred to SP (LD 8:16 hr) on the day of estrus, and groups of animals killed at either 16.00 hr on proestrus or 08.00 hr on estrus during each of five consecutive 4-day estrus cycles after transfer. Groups of females that became anestrus in SP were killed either at 08.00 or 16.00 hr, 12 days after the last observed estrus discharge. Compared to LP controls, serum FSH levels on estrus increased significantly (P<0.01) during the first two cycles in SP before declining to concentrations that were significantly lower than control values (P<0.01). Serum inhibin levels increased significantly by the third, fourth, and five days of estrus in SP. Regression analysis revealed a significant inverse correlation between serum inhibin and FSH levels on estrus during SP exposure (P=0.021) but not on proestrus. Relative levels of inhibin alpha- and betaA-subunit mRNAs were lower in ovaries from SP proestrus and anestrus females killed at 16.00 hr as compared to those from proestrus LP controls; they were elevated in ovaries from SP estrus and anestrus females killed at 08.00 hr compared to those from estrus LP controls. The absence of antral follicles on estrus in the last cycles of SP and anestrus suggests that the increase in circulating inhibin and inhibin mRNAs may be derived from hyperplastic interstitium. These observations suggest that inhibin may play an essential role in suppressing FSH secretion during pineal gland-mediated anestrus in Syrian hamsters.
Benson
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Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson 85724-5044, USA. bbenson@u.arizona.edu
McAsey
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ME
D000780:Anestrus; D000818:Animals; D006224:Cricetinae; D004971:Estrus / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood*; D007265:Inhibins / Q000235:genetics / Q000502:physiology*; D008550:Melatonin / Q000378:metabolism; D008647:Mesocricetus; D010053:Ovary / Q000378:metabolism*; D017440:Photoperiod; D010870:Pineal Gland / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D012098:Reproduction / Q000502:physiology
D012333:RNA, Messenger; D007265:Inhibins; D005640:Follicle Stimulating Hormone; D008550:Melatonin