SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors.
The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30, SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.
Laherty
C D
CD
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Billin
A N
AN
Lavinsky
R M
RM
Yochum
G S
GS
Bush
A C
AC
Sun
J M
JM
Mullen
T M
TM
Davie
J R
JR
Rose
D W
DW
Glass
C K
CK
Rosenfeld
M G
MG
Ayer
D E
DE
Eisenman
R N
RN
D000595:Amino Acid Sequence; D000818:Animals; D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000502:physiology*; D006655:Histone Deacetylases / Q000235:genetics / Q000502:physiology*; D018398:Homeodomain Proteins / Q000502:physiology*; D006801:Humans; D007668:Kidney; D046911:Macromolecular Substances; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D046912:Multiprotein Complexes; D009687:Nuclear Proteins / Q000502:physiology*; D056971:Nuclear Receptor Co-Repressor 1; D011960:Receptors, Estrogen / Q000037:antagonists & inhibitors; D018168:Receptors, Retinoic Acid / Q000502:physiology; D011988:Receptors, Thyroid Hormone / Q000502:physiology; D012097:Repressor Proteins / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins; D050817:Transcription Factor Pit-1; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic; D014162:Transfection
D004268:DNA-Binding Proteins; D006655:Histone Deacetylases; D018398:Homeodomain Proteins; D046911:Macromolecular Substances; D046912:Multiprotein Complexes; D009687:Nuclear Proteins; D056971:Nuclear Receptor Co-Repressor 1; D011960:Receptors, Estrogen; D018168:Receptors, Retinoic Acid; D011988:Receptors, Thyroid Hormone; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D050817:Transcription Factor Pit-1; D014157:Transcription Factors; C539127:NCOR1 protein, human; C539128:Ncor1 protein, mouse; C494187:POU1F1 protein, human; C494185:Pou1f1 protein, mouse; C113294:SAP30 protein, human; C071731:SIN3 protein, S cerevisiae; C491870:Sap30 protein, mouse