A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.
Our previous work identified a cosmid clone containing a 43-kb insert from Enterococcus faecalis OG1RF that produced a nonprotein antigen in Escherichia coli. In the present work, we studied this clone in detail. Periodate treatment of lysates of the clone confirmed that the antigen was carbohydrate in nature. Analysis of DNA sequences and transposon insertion mutants suggested that the insert contained a multicistronic gene cluster. Database comparison showed that the cluster contained genes similar to genes involved in the biosynthesis of dTDP-rhamnose, glycosyltransferases, and ABC transporters involved in the export of sugar polymers from both gram-positive and gram-negative organisms. Insertions in several genes within the cluster abolished the immunoreactivity of the clone. This is the first report on a gene cluster of E. faecalis involved in the biosynthesis of an antigenic polysaccharide.
Xu
Y
Y
Department of Biochemistry and Molecular Biology, University of Texas Medical School, Houston, Texas 77030, USA.
Murray
B E
BE
Weinstock
G M
GM
D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D016208:Databases, Factual; D013293:Enterococcus faecalis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D010504:Periodic Acid; D011134:Polysaccharides / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D017422:Sequence Analysis, DNA
D000942:Antigens, Bacterial; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D011134:Polysaccharides; D010504:Periodic Acid; C009288:metaperiodate