Homologous pairing in stretched supercoiled DNA.
By using elastic measurements on single DNA molecules, we show that stretching a negatively supercoiled DNA activates homologous pairing in physiological conditions. These experiments indicate that a stretched unwound DNA locally denatures to alleviate the force-driven increase in torsional stress. This is detected by hybridization with 1 kb of homologous single-stranded DNA probes. The stretching force involved (approximately 2 pN) is small compared with those typically developed by molecular motors, suggesting that this process may be relevant to DNA processing in vivo. We used this technique to monitor the progressive denaturation of DNA as it is unwound and found that distinct, stable denaturation bubbles formed, beginning in A+T-rich regions.
Strick
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TR
Laboratoire de Physique Statistique, Ecole Normale Superieure, 24 rue Lhomond, 75006 Paris, France. strick@clipper.ens.fr
Croquette
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V
Bensimon
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D
D001482:Base Composition; D015342:DNA Probes; D004278:DNA, Superhelical / Q000235:genetics*; D009693:Nucleic Acid Hybridization
D015342:DNA Probes; D004278:DNA, Superhelical