The pcd gene encoding piperideine-6-carboxylate dehydrogenase involved in biosynthesis of alpha-aminoadipic acid is located in the cephamycin cluster of Streptomyces clavuligerus.
Three open reading frames (ORFs) have been located downstream of cefE in the cephamycin C gene cluster of Streptomyces clavuligerus. ORF13 (pcd) encodes a 496-amino-acid protein (molecular weight [MW], 52,488) with an N-terminal amino acid sequence identical to that of pure piperideine-6-carboxylate dehydrogenase. ORF14 (cmcT) encodes a 523-amino-acid protein (MW, 54,232) analogous to Streptomyces proteins for efflux and resistance to antibiotics. ORF15 (pbp74) encodes a high molecular weight penicillin-binding protein (MW, 74, 094).
Pérez-Llarena
F J
FJ
Area of Microbiology, Faculty of Biology, University of León, 24071 León, Spain.
Rodríguez-García
A
A
Enguita
F J
FJ
Martín
J F
JF
Liras
P
P
D015074:2-Aminoadipic Acid / Q000096:biosynthesis*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D002513:Cephamycins / Q000378:metabolism*; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D011392:Proline / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013302:Streptomyces / Q000235:genetics*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002513:Cephamycins; D004269:DNA, Bacterial; D015074:2-Aminoadipic Acid; D011392:Proline; D000587:Oxidoreductases Acting on CH-NH Group Donors; C109029:delta-1-piperideine-6-carboxylate dehydrogenase; C024925:cephamycin C