IGF-I enhances survival of embryonic chick ciliary ganglion neurons in a calcium-dependent way.
We have shown that neurons from embryonic chick ciliary ganglia in primary culture possess receptors for insulin-like growth factor I (IGF-I). When added to serum- and insulin-free culture medium, the factor potently enhanced neuronal survival as observed after 24 and 48 h of culture. The effect saturated at 5 ng/ml. Laminin was not necessary for the trophic effects of IGF-I; in the absence of the factor, it had no effect on neuronal survival. Insulin exerted a trophic effect similar to that observed with IGF-I, but at higher doses. The trophic effect of IGF-I was sharply and specifically reduced when either a membrane-permeable calcium chelating agent or blockers of voltage-dependent calcium channels were added to the medium.
Department of Animal and Human Biology, University of Torino, Italy.
E
E
Barale
M
M
Torre
C
C
Haimann
D
D
Lovisolo
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000502:physiology*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002614:Chelating Agents / Q000494:pharmacology; D002642:Chick Embryo; D003470:Culture Media; D005726:Ganglia, Parasympathetic / Q000166:cytology* / Q000187:drug effects; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007797:Laminin / Q000096:biosynthesis; D009474:Neurons / Q000187:drug effects / Q000502:physiology*
D002121:Calcium Channel Blockers; D002614:Chelating Agents; D003470:Culture Media; D007797:Laminin; D007334:Insulin-Like Growth Factor I; D002118:Calcium