The effect of retinoic acid on the activation of the human H19 promoter by a 3' downstream region.
The human H19 is paternally imprinted (maternally expressed). It is transcribed by RNA pol II, but has no protein product. Its function is unknown. We showed that the transcription of the human H19 gene is under the simultaneous control of both a 5' upstream (promoter) region and a 3' downstream region in cell lines derived from human choriocarcinomas. Moreover, the activation of the H19 promoter by retinoic acid in cells derived from human testicular germ cell tumors is dependent upon the 3' downstream region. The possibility that the action of retinoic acid on the H19 promoter is an indirect one and involves a member of the AP2 transcription factor family is discussed.
The Department of Biological Chemistry, the Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Israel.
E
E
Kopf
O
O
Bibi
S
S
Ayesh
M
M
Tykocinski
K
K
Vitner
L H
LH
Looijenga
N
N
de Groot
A
A
Hochberg
D000970:Antineoplastic Agents / Q000494:pharmacology*; D015500:Chloramphenicol O-Acetyltransferase / Q000187:drug effects / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000187:drug effects / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009124:Muscle Proteins / Q000187:drug effects / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011994:Recombinant Proteins / Q000187:drug effects / Q000235:genetics; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D014162:Transfection; D014212:Tretinoin / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; C094335:H19 long non-coding RNA; D009124:Muscle Proteins; D062085:RNA, Long Noncoding; D022661:RNA, Untranslated; D011994:Recombinant Proteins; D014212:Tretinoin; D015500:Chloramphenicol O-Acetyltransferase