Influence of temperature upon protein partitioning in poly(ethylene glycol)-salt aqueous two-phase systems close to the critical point with some observations relevant to the partitioning of particles.
The partition of proteins in a poly(ethylene glycol) (PEG)-potassium phosphate aqueous two-phase system has been studied in systems which were produced by manipulation of the equilibrium temperature of a monophasic mixture composition close to the critical point. Precise control of the composition enabled the formation of biphasic systems lying very close to the critical point. A high-performance liquid chromatographic method for analysis of the phase compositions is presented. The generally applicability of the method to the partition of proteins and other particles is discussed.
de Belval
S
S
Biochemical Recovery Group, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
le Breton
B
B
Huddleston
J
J
Lyddiatt
A
A
D002851:Chromatography, High Pressure Liquid; D008956:Models, Chemical; D010710:Phosphates / Q000737:chemistry; D011092:Polyethylene Glycols; D017680:Potassium Compounds / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012492:Salts; D013050:Spectrometry, Fluorescence; D013696:Temperature
D010710:Phosphates; D017680:Potassium Compounds; D011506:Proteins; D012492:Salts; D011092:Polyethylene Glycols; C013216:potassium phosphate