Regulation of gene expression in vivo following transduction by two separate rAAV vectors.
Control of gene expression is important to gene therapy for purposes of both dosing and safety. In vivo regulation of gene expression was demonstrated following co-injection of two separate recombinant adeno-associated virus vectors, one encoding an inducible murine erythropoietin transgene and the other a transcriptional activator, directly into the skeletal muscle of adult immunocompetent mice. Transcription was controlled by systemic administration or withdrawal of tetracycline over an 18 week period, demonstrating that the two vectors were capable of transducing the same cell. Cellular or humoral immune responses against the transactivator protein were not detected.
Rendahl
K G
KG
Cell Genesys Inc., Foster City, CA 94404, USA.
Leff
S E
SE
Otten
G R
GR
Spratt
S K
SK
Bohl
D
D
Van Roey
M
M
Donahue
B A
BA
Cohen
L K
LK
Mandel
R J
RJ
Danos
O
O
Snyder
R O
RO
D016475:3T3 Cells; D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D000229:Dependovirus / Q000235:genetics*; D004921:Erythropoietin / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D006400:Hematocrit; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D013752:Tetracycline / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics; D019076:Transgenes
D015534:Trans-Activators; D004921:Erythropoietin; D013752:Tetracycline