The expression of beta-microseminoprotein gene is regulated by cAMP.
The promoter of the human gene for beta-microseminoprotein (MSP) was analyzed by transient transfection assay using LNCaP cells. The region between -2738 and -207 was not essential for the basal promoter activity, but regions from -206, -108, and -83 were necessary. Forskolin induced promoter activity to 3- to 14-fold when constructions containing the possible cAMP response element (CRE, -60 to -53) were used, but did not do so when it was deleted from them. In a gel-mobility shift assay using a probe corresponding to -68 to -48, the shifted band was competed with a consensus CRE, and supershifted when the anti-CREB-1 antibody was included. Forskolin added to LNCaP cells increased MSP mRNA to 6-fold within 12 h. These results suggest that the expression of MSP gene is regulated by cAMP via the CRE present in the promoter. Hormones such as (-)-epinephrine or vasoactive intestinal polypeptide also increased MSP mRNA to 2- to 3-fold.
Department of Medical Biochemistry, Shiga University of Medical Science, Otsu, Japan.
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Ueyama
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Ohkubo
D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007265:Inhibins / Q000096:biosynthesis; D007700:Kinetics; D008156:Luciferases / Q000096:biosynthesis; D008297:Male; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms; D029584:Prostatic Secretory Proteins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D017384:Sequence Deletion; D014162:Transfection; D014407:Tumor Cells, Cultured
D010455:Peptides; D029584:Prostatic Secretory Proteins; D011993:Recombinant Fusion Proteins; C043076:beta-microseminoprotein; D005576:Colforsin; D007265:Inhibins; D000242:Cyclic AMP; D008156:Luciferases