Mobilized plasma lead as an index of lead body burden and its relation to the heme-related indices.
Plasma lead (Pb-P) from workers were distributed in two main fractions: a protein bound fraction and low molecular weight fractions. Lead mobilized into plasma by CaEDTA was mainly observed in the low molecular weight fraction corresponding to lead disodium ethylenediamine tetraacetic acid (PbEDTA). The peak levels of Pb-P was attained around 1.5 and 2.5 hours after the start of CaEDTA injection. Pb-P and blood lead levels (Pb-B) at 2 h after the injection were 4.26 (+/- 2.84) and 0.96 (+/- 0.27) fold of the initial levels just before the injection. Pb-P concentrations at 2 hours after the start of CaEDTA injection (MPb-P) were well correlated (r = 0.740) with amounts of lead excreted in urine for 24 h thereafter (MPb-U). log MPb-P as well as log MPb-U were correlated with Pb-B (r = 0.765 and 0.817, respectively). Correlation coefficients of lead body burden (MPb-P or MPb-U) vs the logarithms of the effect indices (delta-aminolevulinic acid (ALA) dehydratase, ALA in urine, coproporphyrin in urine, and erythrocyte zinc protoporphyrin) were higher than the correlation coefficients of exposure indices (Pb-B or Pb-U) vs the logarithms of the effect indices. Thus the biological effect monitoring is significant and reliable for evaluating the functional components of lead body burden (MPb-P or MPb-U).
Center of Occupational Medicine, Tokyo Labor Accident Hospital, Japan.
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Sakai
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Ushio
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Ikeya
D000328:Adult; D000818:Animals; D002850:Chromatography, Gel; D004492:Edetic Acid / Q000008:administration & dosage*; D004791:Enzyme Inhibitors / Q000097:blood / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D007262:Infusions, Intravenous; D007854:Lead / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D000623:Porphobilinogen Synthase / Q000097:blood / Q000378:metabolism; D011524:Protoporphyrins / Q000097:blood / Q000378:metabolism; D013054:Spectrophotometry, Atomic
D004791:Enzyme Inhibitors; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D007854:Lead; D004492:Edetic Acid; D000623:Porphobilinogen Synthase