Probing the receptor recognition site of the FimH adhesin by fimbriae-displayed FimH-FocH hybrids.
Type 1 fimbriae are surface organelles of Escherichia coli which mediate D-mannose-sensitive binding to different host surfaces. This binding is conferred by the minor fimbrial component FimH. The binding domain of the FimH adhesin has been studied by constructing hybrids of FimH and a homologous protein, FocH, originating from F1C fimbriae. F1C fimbriae do not bind to D-mannosides or confer agglutination of any known types of erythrocytes or yeast. It was previously shown that the FocH protein can be readily substituted by the FimH adhesin, resulting in hybrid fimbriae with the same binding characteristics as type 1 fimbriae. The receptor binding of fimbriae-presented chimeric FimH-FocH hybrids was studied. FimH-FocH fusions encompassing 72% of the N-terminus of FimH fused to the complementary sector of FocH conferred agglutination of erythrocytes and yeast cells at a comparable level to FimH. Surprisingly, it was also found that similar fusions containing between 56 and 66% FimH still conferred binding to yeast cells, D-mannose-BSA and D-mannose-beads but did not give rise to agglutination. The receptor binding capacity of fusions containing 50% or less of the FimH N-terminal region was virtually abolished. The results point to the presence of a D-mannose-receptor-binding core domain in FimH, the affinity of which is modulated by other sectors of the protein to enable binding to extended mannose-containing targets.
Thomas B
TB
Knudsen
Per
P
Klemm
D018830:Adhesins, Escherichia coli / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D000371:Agglutination; D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured / Q000276:immunology; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D010861:Fimbriae, Bacterial / Q000235:genetics* / Q000378:metabolism; D006168:Guinea Pigs; D008362:Mannosides / Q000032:analysis / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000378:metabolism; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid; D015003:Yeasts / Q000276:immunology
D018830:Adhesins, Escherichia coli; D008362:Mannosides; D011993:Recombinant Fusion Proteins