Oncogene amplification and expression in pediatric solid tumors.
Oncogene amplification and expression and their mutual relationship was analyzed in 92 pediatric tumors by Southern and Northern blot hybridization with N-MYC, ERB A, ERB B, N-RAS and Shb probes. Amplification and overexpression was associated with more advanced clinical stages of tumor, especially in neuroblastomas, rhabdomyosarcomas and ganglioneuroblastomas. The most frequent alteration observed was N-MYC amplification together with overexpression. N-RAS amplification was not detected, while the overexpression of this oncogene was found in 3 cases. Neither amplification nor overexpression was revealed in any specimen of hepatoblastoma or hepatocellular carcinoma. We suggest that oncogenes overexpression provides more accurate prognostic information than amplification.
Institute of Biology and Medical Genetics, 2nd Medical School, Charles' University, Prague, Czech Republic.
J
J
Mares
V
V
Polanská
H
H
Görgens
Z
Z
Sedlácek
T
T
Maríková
P
P
Bocek
R
R
Kodet
J
J
Schackert
P
P
Goetz
D002648:Child; D002675:Child, Preschool; D005784:Gene Amplification; D015870:Gene Expression; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D009857:Oncogenes; D016133:Polymerase Chain Reaction