Separation and purification of glucoamylase in aqueous two-phase systems by a two-step extraction.
Extraction in two steps of glucoamylase was studied in poly(ethylene glycol) (PEG) and potassium phosphate systems at pH values of 6, 7 and 9. Ten different conditions using PEG 300, 600, 1500, 4000 and 6000 were studied. The bottom phase of the first extraction step, with the enzyme, was reused in an appropriate concentration of PEG to form the second extraction step. The optimal partitioning conditions for glucoamylase separation were obtained in PEG 4000 (first step), PEG 1500 (second step) at pH 7 and resulted in a three-fold increase in glucoamylase purification.
Departamento de Engenharia Química, Escola Politécnica, Universidade de São Paulo, S.P., Brazil.
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NM
Minami
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Kilikian
D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005087:Glucan 1,4-alpha-Glucosidase / Q000302:isolation & purification*; D010710:Phosphates; D011092:Polyethylene Glycols; D017680:Potassium Compounds; D014867:Water
D010710:Phosphates; D017680:Potassium Compounds; D014867:Water; D011092:Polyethylene Glycols; C013216:potassium phosphate; D005087:Glucan 1,4-alpha-Glucosidase