Analysis of CD34-expressing cells in clinical practice.
The flow cytometric determination of haemopoietic cells defined as CD34-expressing cells has greatly added to the improvement of the management of harvesting circulating haemopoietic cells for subsequent autologous reinfusion in the setting of high-dose chemo/(radio)-therapy. Additionally, this flow cytometric determination has replaced, in some institutions, the in-vitro culture test for CFU-GM as the measure to estimate the haemopoietic potential of the cells to be reinfused/transplanted.
Serke
S
S
Department of Haematology-Oncology, Campus Virchow Hospital Charite, Humboldt University, Berlin, Germany.
Beyer
J
J
Rick
O
O
Schwella
N
N
Meyer
O
O
Hildebrandt
M
M
Huhn
D
D
D018952:Antigens, CD34 / Q000032:analysis*; D000943:Antigens, Differentiation / Q000032:analysis; D017209:Apoptosis; D001835:Body Weight; D002452:Cell Count / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation; D003114:Colony-Forming Units Assay; D005434:Flow Cytometry; D006085:Graft Survival; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans
D018952:Antigens, CD34; D000943:Antigens, Differentiation