Antigenic characterization of nine wild-type Taiwanese isolates of Japanese encephalitis virus as compared with two vaccine strains.
The antigenic properties of nine wild-type Japanese encephalitis viruses isolated in Taiwan during 1990 1994 were investigated by comparison with two inactivated vaccine strains (Beijing-1, Nakayama-NIH). All of the nine Taiwanese isolates were found to induce higher cytopathology in Vero cells but showed similar mouse virulence as the two vaccine strains. Antigenic characterization using six E protein-specific monoclonal antibodies shows two of the nine wild-type isolates (i.e. CH1949 and CH2195) presented different antigenic properties of hemagglutination inhibition and plaque reduction neutralization. The E-protein gene nucleotide sequences of CH1949 and CH2195 were determined and compared with other published sequences of the two vaccine strains and other 19 Asian/Taiwanese isolates. Phylogenetic tree analysis indicates these two wild-type Taiwanese isolates are more distant from the two vaccine strains.
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan. scwu@life.nthu.edu.tw
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Wu
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Lian
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Hsu
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Wu
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Liau
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000276:immunology*; D015153:Blotting, Western; D001921:Brain / Q000821:virology; D002460:Cell Line; D003465:Culex / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D004664:Encephalitis Virus, Japanese / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008813:Mice, Inbred ICR; D009500:Neutralization Tests; D010802:Phylogeny; D013624:Taiwan; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*; D010948:Viral Plaque Assay; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D014774:Virulence
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C080628:glycoprotein E, Japanese encephalitis virus