Incorporation of nisin into a meat binding system to inhibit bacteria on beef surfaces.
In two separate experiments, the bacteriocin, nisin, was incorporated into a commercially available meat binding system (Fibrimex) and applied to meat surfaces as a way of inhibiting the meat spoilage organism, Brochothrix thermosphacta during extended refrigerated storage. In experiment 1, pre-rigor lean beef carcass tissue (BCT) was inoculated with B. thermosphacta, left untreated (U), treated with 10 micrograms ml-1 nisin (N), Fibrimex (F) or Fibrimex containing 10 micrograms ml-1 nisin (FN), held aerobically at 4 degrees C for up to 7 d, and populations of B. thermosphacta and nisin activity determined. Experiment 2 determined the effects of the same treatments but on post-rigor, frozen and thawed lean BCT that was inoculated, vacuum-packaged, and stored at 4 degrees C for up to 14 d. In both experiments, N- and FN-treated tissues exhibited significantly lower populations of B. thermosphacta compared to U- and F-treated tissues, for the duration of refrigerated storage. Nisin activity was detected up to 7 d in N- and FN-treated samples from experiment 1. However, activity was detected only to days 0 and 2 in FN- and N-treated samples, respectively, from experiment 2. These studies indicate that the addition of a bacteriocin to a meat binding system and application to meat surfaces may be useful in reducing undesirable bacteria in restructured meat products.
Cutter
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United States Department of Agriculture, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, NE, USA. cutter@email.marc.usda.gov
Siragusa
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D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D015169:Colony Count, Microbial; D005506:Food Contamination / Q000517:prevention & control*; D005511:Food Handling / Q000379:methods; D005519:Food Preservation / Q000379:methods; D005520:Food Preservatives / Q000008:administration & dosage*; D006093:Gram-Positive Asporogenous Rods / Q000187:drug effects / Q000254:growth & development; D008460:Meat / Q000382:microbiology*; D009561:Nisin / Q000008:administration & dosage*
D005520:Food Preservatives; D009561:Nisin