[Tissue engineering of cartilagenous tissue. Vital cryopreservation].
The characteristics of in vitro synthesized cartilage tissue using tissue engineering techniques before and after cryopreservation are described. We determined cell survival, growth of extracellular matrix (collagen and proteoglycan) using a computer analysis system, and characterized the cell phenotype with a monoclonal antibody specific for collagen type II. Chondrocytes maintained a differentiated phenotype with collagen and proteoglycan synthesis before and after cryopreservation.
Servicio de ORL, Hospital General Universitario, Murcia.
E
E
Viviente Rodríguez
H
H
Sudhoff
A
A
Capitán
C
C
Torregrosa
C
C
Sprekelsen
J
J
Bujía
D002356:Cartilage; D015925:Cryopreservation; D046508:Culture Techniques; D005615:Freezing; D006801:Humans; D014021:Tissue Preservation