High resolution capillary electrophoretic separation of oligonucleotides in low-viscosity, hydrophobically end-capped polyethylene oxide with cubic order.
A triblock self-associating polymer with the structure n-dodecane-poly(ethylene oxide)-n-dodecane and a very low polydispersity has been used as a matrix to separate a sample of single-stranded oligonucleotides containing Pd(A)25-30 and Pd(A)40-60. Above a concentration of 4%, this associative polymer forms a micellar network with cubic order and a well-defined micellar spacing, in which the dodecane micellar cores are bridged by polyoxyethylene segments. This medium combines a low viscosity with excellent resolution of oligonucleotides. This work confirms that associative polymers are potentially powerful media for separation in capillary electrophoresis, and argues in favor of the use of monodisperse products presenting a high-order in the physical gel state.
Laboratoire de physico-chimie Curie (UMR CNRS 168) Institut Curie, Section de Recherche, Paris, France.
S
S
Magnusdottir
J L
JL
Viovy
J
J
François
D019075:Electrophoresis, Capillary / Q000379:methods*; D009841:Oligonucleotides / Q000302:isolation & purification*; D011061:Poly A / Q000302:isolation & purification; D011092:Polyethylene Glycols; D014783:Viscosity
D009841:Oligonucleotides; D011061:Poly A; C015465:poly(dA); D011092:Polyethylene Glycols