[Appearance of glutamate receptors on the surface of HL-60 cells upon differentiation].
A specific interaction of L-glutamic acid with promyelocytic leukemia HL-60 cells completely differentiated by all-E-retinoic acid (Kd = 0.5 microM) and by plasma membrane fraction from the same cells (Kd = 1 microM) was detected through radioligand analysis and characterized. Quisqualate, a nonlabeled structural analogue of glutamic acid, was found to inhibit competitively the specific binding of L-[3H]glutamic acid to the membranes (Ki = 0.24 microM). The stereospecificity of the binding was demonstrated. These data suggest that specific glutamate receptors appear on the surface of HL-60 cells during their differentiation.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
I A
IA
Kostanian
R I
RI
Nurieva
T N
TN
Lepikhova
M V
MV
Astapova
E V
EV
Navolotskaia
V P
VP
Zavialov
V M
VM
Lipkin
D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002458:Cell Fractionation; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D011869:Radioligand Assay; D017470:Receptors, Glutamate / Q000096:biosynthesis* / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology
D017470:Receptors, Glutamate; D018698:Glutamic Acid; D014212:Tretinoin