Agrobacterium VirE2 proteins can form a complex with T strands in the plant cytoplasm.
Wild-type VirE2 and VirD2 proteins from Agrobacterium tumefaciens contain nuclear targeting sequences (NLS) that are likely involved in directing transferred T strands to the plant nucleus. An A. tumefaciens virE2 virD2DeltaNLS double mutant was able to form tumors on VirE2-producing transgenic tobacco but not on wild-type tobacco. Because this mutant bacterial strain contains no known T-strand nuclear targeting signal, the data indicate that wild-type VirE2 proteins produced by the plant can interact with the T strands in the plant cytoplasm and direct them to the nucleus.
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA. gelvin@bilbo.bio.purdue.edu
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D016960:Agrobacterium tumefaciens / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism / Q000382:microbiology; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007473:Ion Channels; D009154:Mutation; D019913:Nuclear Localization Signals; D030821:Plants, Genetically Modified; D010947:Plants, Toxic; D014026:Nicotiana / Q000382:microbiology*; D037521:Virulence Factors
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D007473:Ion Channels; D019913:Nuclear Localization Signals; D037521:Virulence Factors; C051583:virE2 protein, Agrobacterium