Cell-mediated immune response of healthy adults to Bordetella pertussis vaccine antigens.
Cell-mediated immunity (CMI) to Bordetella pertussis and acellular pertussis vaccine constituents (pertussis toxin, pertactin, and filamentous hemagglutinin) were studied in peripheral blood mononuclear cells (PBMC) and T cell cultures from healthy adults with no record of vaccination against, or history of, pertussis. Similarly to stimulation with common recall antigens, PBMC proliferation was induced in 80%-100% of the cultures, depending on the specific B. pertussis stimulant. Proliferation did not occur when antigen-presenting cells were ablated by chemical or physical methods or with naive cord blood lymphocytes. B. pertussis antigen stimulation resulted in a preferential induction of type 1 cytokine profile, as shown by interferon-gamma and interleukin-2 (but no interleukin-4 or interleukin-5) gene transcripts and actual cytokine production by T cells. The data suggest that most healthy adults are repeatedly exposed to B. pertussis, with natural acquisition of antigen-specific CMI and a putatively protective type 1 cytokine pattern.
Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, Rome, Italy. ausiello@sun.iss.it
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Ausiello
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la Sala
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Urbani
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Cassone
D018829:Adhesins, Bacterial / Q000276:immunology; D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001886:Bordetella pertussis / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D008875:Middle Aged; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology
D018829:Adhesins, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; C068929:pertactin; D037342:Pertussis Toxin