Endothelial cells cultured from human brain microvessels produce complement proteins factor H, factor B, C1 inhibitor, and C4.
The inflammatory mediators, cytokines and complement proteins are believed to regulate the sequential events during the development of lesions secondary to ischaemia and reperfusion. The endothelial cell monolayer of the brain microvasculature is the critical interface between the blood-borne mediators and brain tissue. The involvement of these cells in complement production and regulation has not been well documented. In the present study, expression of complement proteins (C1 inhibitor, factor H, factor B, C4) by cultured endothelial cells obtained from human brain microvessels has been characterized. Interferon gamma upregulates the production of all the complement factors studied. Serine proteases, plasmin and miniplasmin induce the expression of C4, decrease the level of ELISA detectable C1 inhibitor, and do not affect the production of factors H and B. These data indicate that complement proteins are expressed locally by the brain microvessels, and may modulate the inflammatory responses of brain tissue.
National Stroke Centre, Budapest, Hungary.
M
M
Vastag
J
J
Skopál
J
J
Kramer
K
K
Kolev
Z
Z
Vokó
E
E
Csonka
R
R
Machovich
Z
Z
Nagy
D001921:Brain / Q000098:blood supply*; D002196:Capillaries; D002478:Cells, Cultured; D003174:Complement C1 Inactivator Proteins / Q000096:biosynthesis; D003181:Complement C4 / Q000096:biosynthesis; D011415:Complement Factor B / Q000096:biosynthesis; D017242:Complement Factor H / Q000096:biosynthesis; D003169:Complement Inactivator Proteins / Q000096:biosynthesis*; D003165:Complement System Proteins / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D005341:Fibrinolysin / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology
C493495:CFH protein, human; D003174:Complement C1 Inactivator Proteins; D003181:Complement C4; D003169:Complement Inactivator Proteins; D010446:Peptide Fragments; C019493:miniplasmin; D017242:Complement Factor H; D007371:Interferon-gamma; D003165:Complement System Proteins; D011415:Complement Factor B; D005341:Fibrinolysin