Detection of Lawsonia intracellularis in Hungarian swine herds by polymerase chain reaction.
Pooled faecal samples and/or intestinal contents from pig carcasses were collected from 11 Hungarian swine farms and subjected to DNA extraction/ purification and subsequent polymerase chain reaction (PCR) in order to detect Lawsonia intracellularis, the aetiological agent of the porcine proliferative enteropathy complex. Specific PCR positivity was detected in 6 individual and 22 pooled samples out of 46, originating from eight herds. The PCR products of collected faecal samples hybridised in Southern blot hybridisation with the DNA of the type strain L. intracellularis NCTC 12657. This is the first confirmed detection of L. intracellularis by PCR in Hungary.
Biksi
I
I
Department of Pathology and Forensic Veterinary Medicine, University of Veterinary Science, Budapest, Hungary.
Kacskovics
I
I
Mándoki
M
M
Iván
J
J
Horváth-Papp
I
I
Makay
G
G
Vetési
F
F
D000818:Animals; D015139:Blotting, Southern; D005260:Female; D006090:Gram-Negative Bacteria / Q000302:isolation & purification*; D016905:Gram-Negative Bacterial Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D006814:Hungary / Q000453:epidemiology; D006824:Hybridization, Genetic; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology*