Nuclear magnetic resonance structure of d(GCATATGATAG). d(CTATCATATGC): a consensus sequence for promoters recognized by sigma K RNA polymerase.
The three-dimensional structure of d(GCATATGATAG).d(CTATCATATGC), from the promoter region of a gene regulating sporulation in Bacillus subtilis mother cells, was determined utilizing two-dimensional nuclear Overhauser effect (2D NOE) and double-quantum-filtered COSY (2QF-COSY) spectra. To minimize the effect of methods used to obtain restraints and refine structure, several variables were studied. Interproton distance bounds were calculated very conservatively by running the complete relaxation matrix program MARDIGRAS hundreds of times using 2D NOE spectra for exchangeable and for nonexchangeable protons at different mixing times, assuming different overall correlation times and different starting structures. The 435 distance restraints were used with two different structural refinement methods: restrained molecular dynamics (rMD) and restrained Monte Carlo calculations (rMC). Refinement using different procedures and starting structures resulted in essentially the same structure (<0.8 A rmsd), indicating that the structure is defined by experimental restraints and not the refinement method or variables used. R factors indicate the structures fit the experimental NOE data very well. Some helical parameters, notably large negative X displacement, are characteristic of A-DNA, but others are characteristic of B-DNA. As with TG.CA steps in other duplex DNA sequences studied in our laboratory, the two TG.CA steps have a positive roll, with T6-G7 exhibiting the largest, and consequently a bent helix axis. The converged structure represents a time-averaged structure. However, multiple conformations, especially in deoxyriboses, were evident from vicinal coupling constants obtained from quantitative simulations of 2QF-COSY cross-peaks and from persistent inconsistencies in experimental distances due to nonlinear conformational averaging.
Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0446, USA.
M
M
Tonelli
E
E
Ragg
A M
AM
Bianucci
K
K
Lesiak
T L
TL
James
D001483:Base Sequence; D016384:Consensus Sequence; D018360:Crystallography, X-Ray; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D003854:Deoxyribonucleotides / Q000737:chemistry* / Q000235:genetics; D008432:Mathematical Computing; D008958:Models, Molecular; D009010:Monte Carlo Method; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D009690:Nucleic Acid Conformation; D009692:Nucleic Acid Heteroduplexes / Q000737:chemistry; D011401:Promoter Regions, Genetic; D011522:Protons; D012984:Software; D013816:Thermodynamics; D014157:Transcription Factors / Q000235:genetics*
D003854:Deoxyribonucleotides; D009692:Nucleic Acid Heteroduplexes; D011522:Protons; D014157:Transcription Factors; C084190:sigma K; D012321:DNA-Directed RNA Polymerases