Somatostatin does not attenuate intestinal injury in dextran sodium sulphate-induced subacute colitis.
FRom several in vitro and in vivo studies involvement of somatostatin (SMS) in intestinal inflammation emerge. Acute colitis induced in rats is attenuated by the long-acting SMS analogue octreotide. We studied the potential beneficial effect of SMS on non-acute experimental colitis. BALB/c mice received either saline, SMS-14 (36 or 120 microg daily) or octreotide (3 microg daily) subcutaneously delivered by implant osmotic pumps. A non-acute colitis was induced by administration of dextran sodium sulphate (DSS) 10% in drinking water during 7 days. DSS evoked a mild, superficial pancolitis, most characterized by mucosal ulceration and submucosal influx of neutrophils. Neither SMS-14 nor octreotide reduced mucosal inflammatory score or macroscopical disease activity, although reduction of intestinal levels of interleukin-1beta (IL-1beta), IL-6 and IL-10 during DSS was augmented both by SMS and octreotide. A slight increase of neutrophil influx was seen during SMS administration in animals not exposed to DSS. In conclusion, SMS or its long-acting analogue did not reduce intestinal inflammation in non-acute DSS-induced colitis. According to the cytokine profile observed, SMS-14 and octreotide further diminished the reduction of intestinal macrophage and Th2 lymphocyte activity.
van Bergeijk
J D
JD
Department of Gastroenterology/Internal Medicine, University Hospital Rotterdam, The Netherlands.
van Meeteren
M E
ME
Tak
C J
CJ
van Dijk
A P
AP
Meijssen
M A
MA
Wilson
J H
JH
Zijlstra
F J
FJ
D000818:Animals; D003092:Colitis / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D016264:Dextran Sulfate / Q000633:toxicity; D005260:Female; D007375:Interleukin-1 / Q000032:analysis; D016753:Interleukin-10 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D013004:Somatostatin / Q000627:therapeutic use*
D007375:Interleukin-1; D015850:Interleukin-6; D016753:Interleukin-10; D013004:Somatostatin; D016264:Dextran Sulfate