An alpha-L-fucosidase from Penicillium multicolor as a candidate enzyme for the synthesis of alpha (1-->3)-linked fucosyl oligosaccharides by transglycosylation.
A new alpha-L-fucosidase was partially purified from the culture broth of Penicillium multicolor, which was available commercially as a freeze dried powder by the name of Lactase-P. This enzyme catalysed the transglycosylation of fucose residue of p-nitrophenyl-alpha-L-fucopyranoside to give alpha-L-Fuc-(1-->3)-D-Glc or alpha-L-Fuc-(1-->3)-D-GlcNAc regioselectively. This enzyme was more stable in the organic co-solvents than the alpha-fucosidase from Aspergillus niger, which was also proposed previously by us as an enzyme to produce fucosyl oligosaccharides.
Ajisaka
K
K
Meiji Institute of Health Science, Meiji Milk Products, Co., Ltd., Odawara, Japan. rxg0120@niftyserve.or.jp
Fujimoto
H
H
Miyasato
M
M
D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000096:biosynthesis; D006031:Glycosylation; D007700:Kinetics; D008970:Molecular Weight; D010407:Penicillium / Q000201:enzymology*; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D012997:Solvents; D013379:Substrate Specificity; D005644:alpha-L-Fucosidase / Q000302:isolation & purification / Q000378:metabolism*
D004187:Disaccharides; D011135:Polysaccharides, Bacterial; D012997:Solvents; D005644:alpha-L-Fucosidase