Evidence for the existence of cytochrome b558 in fish neutrophils by polyclonal anti-peptide antibody.
When western blotting analysis of Japanese eel neutrophil lysate was performed using antibody against the synthetic peptide corresponding to the carboxyl terminal region of human cytochrome b558 large subunit, a broad band was specifically detected at approximately 90 kDa. The antibody recognized the epitope present in eel neutrophils only after the cells were permeabilized with detergent. These results indicate that the large subunit of cytochrome b exists in fish neutrophils and the epitope is exposed to the cytoplasmic side of membrane, and suggest that cytochrome b is conserved across species.
Itou
T
T
United Graduate School of Agricultural Sciences, Kagoshima University, Japan.
Iida
T
T
Kawatsu
H
H
D000818:Animals; D015153:Blotting, Western; D003573:Cytochrome b Group / Q000378:metabolism*; D004524:Eels; D000939:Epitopes; D019084:Fluorescent Antibody Technique, Indirect; D019255:NADPH Oxidases / Q000378:metabolism; D009504:Neutrophils / Q000201:enzymology*; D010446:Peptide Fragments / Q000276:immunology
D003573:Cytochrome b Group; D000939:Epitopes; D010446:Peptide Fragments; C036888:cytochrome b558; D019255:NADPH Oxidases