[PCR analysis of microsatellite DNA markers: possibility of erroneous determination of genotypes].
A comparative study of two techniques for the PCR genotyping of highly polymorphic tandem repeats was carried out by the example of a triplet repeat in the myotonin protein kinase gene. Sequencing denaturing gels were shown to yield more precise results in the analysis of amplification products.
Popova
S N
SN
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, Russia.
Shadrina
M I
MI
Fatkhlislamova
R I
RI
Khusnutdinova
E K
EK
Slominskiĭ
P A
PA
D004276:DNA, Satellite / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D005819:Genetic Markers; D005838:Genotype; D065636:Myotonin-Protein Kinase; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D011494:Protein Kinases / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D012091:Repetitive Sequences, Nucleic Acid
D004276:DNA, Satellite; D005819:Genetic Markers; D011494:Protein Kinases; D065636:Myotonin-Protein Kinase; D017346:Protein Serine-Threonine Kinases