Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.
Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.
Berridge
B R
BR
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA. bberridge@cvm.tamu.edu
Fuller
J D
JD
de Azavedo
J
J
Low
D E
DE
Bercovier
H
H
Frelier
P F
PF
D000818:Animals; D000829:Animals, Domestic; D016470:Bacteremia / Q000382:microbiology / Q000401:mortality / Q000662:veterinary; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics*; D005393:Fish Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000401:mortality; D005399:Fishes / Q000382:microbiology*; D006801:Humans; D008590:Meningoencephalitis / Q000382:microbiology / Q000401:mortality / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000401:mortality / Q000662:veterinary*; D013291:Streptococcus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
D017931:DNA Primers; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S