Immunological analysis of enhanced spontaneous metastasis in WKA rats following cryosurgery.
We have previously reported that inhibition of anti-tumor immune responses and enhancement of metastatic tumor growth occurred in rats following cryosurgery of the transplantable 3-methlcholanthrene-induced rat fibrosarcoma KMT-17. In this study, to elucidate the immunological responses in rats following cryosurgery, we examined whether rat serum obtained from rats which underwent cryosurgery (c-serum) might affect the in vivo neutralizing activity of the Winn assay. In this assay, c-serum did not reduce the anti-tumor immunity, though spleen cells obtained from rats undergoing surgical excision indicated strong anti-tumor immunity as compared with cryosurgery. Thus, we examined the anti-tumor responses of spleen cells. Macrophages were obtained from the glass adherent fraction of rat spleen cells following cryosurgery and these macrophages were used for cytostatic activity against KMT-17 cells. Cytostatic activity was not reduced by cryosurgery. The spleen cells obtained from rats receiving cryosurgery were intravenously transferred into other rats that were previously immunized with 80 Gy-irradiated KMT-17 cells, and an alteration of tumor growth modulated by this adoptive cell transfusion was observed. The anti-tumor resistance of rats was diminished by the adoptive transfusion of spleen cells treated with cryosurgery, though this diminution disappeared following anti-T serum and immune complement treatment of spleen cells. These results suggest that immuno-suppression following cryosurgery may be mainly caused by suppressor T cells.
Shibata
T
T
Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido, Japan. shibata@hoku-iryo-u.ac.jp
Suzuki
K
K
Yamashita
T
T
Takeichi
N
N
Mark
M
M
Hosokawa
M
M
Kobayashi
H
H
Arisue
M
M
D019264:Adoptive Transfer; D000818:Animals; D002455:Cell Division; D003452:Cryosurgery; D005260:Female; D005354:Fibrosarcoma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology* / Q000601:surgery; D008264:Macrophages / Q000276:immunology*; D008748:Methylcholanthrene; D009362:Neoplasm Metastasis; D051381:Rats; D017208:Rats, Wistar; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D008748:Methylcholanthrene