A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence.
A new screening method for amphetamines was developed. It consists of derivatization with dansyl chloride, extraction of the derivative using a Sep-Pak C18 or a Bond Elut C18, solid phase extraction columns, and visualization of the fluorescence of the cartridge. A control test using drug-free urine showed no fluorescence. Amphetamine, methamphetamine and the methylenedioxy derivatives exhibited strong fluorescence, while related compounds, such as N-ethylamphetamine and fenetylline, were negative or weakly positive. The disadvantage of the present method is that it is a multi-step procedure and 20-30 min is required for screening. However, since it has a different specificity from the widely used immunochemical technique, it is suggested to be a useful screen for amphetamines.
Laboratory of Hygienic and Forensic Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
H
H
Yamada
S
S
Ikeda-Wada
K
K
Oguri
D000661:Amphetamine / Q000652:urine*; D003619:Dansyl Compounds / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D008694:Methamphetamine / Q000652:urine*; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
D003619:Dansyl Compounds; D008694:Methamphetamine; D000661:Amphetamine; C029038:dansyl chloride