Interspersed centromeric element with a CENP-B box-like motif in Chironomus pallidivittatus.
Short mobile elements are present in different recombined forms as interspersed GC-rich islands between AT rich centromeric 155 bp tandem repeats in the dipteran Chironomus pallidivittatus . The basic element is 80 bp long, has a pronounced invert repeat structure and contains a 17 bp segment similar to the CENP-B box in mammals. The element inserts into a specific site of the 155 bp repeat in a defined orientation surrounded by 2 bp direct repeats. The total number per genome of the main variant is <20. Elements can be present in all centromeres from C.pallidivittatus and the sibling species Chironomus tentans with pronounced differences in distribution within and between species.
Department of Genetics, Lund University, Sölvegatan 29, S-22362 Lund, Sweden.
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López
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Edström
D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D002503:Centromere / Q000235:genetics*; D051936:Centromere Protein B; D002683:Chironomidae / Q000235:genetics* / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis / Q000235:genetics*; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins; D014644:Genetic Variation; D015698:Genomic Library; D017404:In Situ Hybridization, Fluorescence; D020071:Interspersed Repetitive Sequences; D007814:Larva; D008322:Mammals; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis; D012469:Salivary Glands / Q000166:cytology / Q000378:metabolism; D013045:Species Specificity
D001324:Autoantigens; D051936:Centromere Protein B; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins