Mapping of calpastatin and three microsatellites to porcine chromosome 2q2.1-q2.4.
Three polymorphisms were identified in a 1.6-kb fragment of the porcine calpastatin (CAST) gene and these polymorphisms were used for genetic linkage mapping. Linkage analysis revealed significant linkage of CAST to five microsatellites previously mapped to porcine chromosome 2; these microsatellites were S0010, S0226, SWI4, Sw395 and Sw776. A somatic cell hybrid panel was used to determine the chromosomal localization of CAST and the microsatellites S0091, S0226 and Sw395. All of these were localized to the region 2q2.1-q2.4.
Department of Animal Science, Iowa State University, Ames 50011, USA.
C W
CW
Ernst
A
A
Robic
M
M
Yerle
L
L
Wang
M F
MF
Rothschild
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics*; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D006822:Hybrid Cells; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D013552:Swine / Q000235:genetics*
D002135:Calcium-Binding Proteins; D005819:Genetic Markers; C033668:calpastatin