Sequence and diversity of T-cell receptor alpha V, J, and C genes of the owl monkey Aotus nancymaae.
We cloned and sequenced TcR alpha chain cDNA of three healthy Aotus nancymaae monkeys. Fifteen different TRAJ segments and 9 different TRAV genes were identified in the 29 rearrangements analyzed. As expected from the greater phylogenetic distance, A. nancymaae TRA gene sequences diverged more from the human sequences than those of the chimpanzee or the rhesus macaque. However, no Aotus TRAJ segment or TRAV gene was found which lacked a human counterpart. These counterparts were AJ02, AJ05, AJ09, AJ15, AJ22, AJ23, AJ28, AJ30, AJ32, AJ34, AJ37, AJ40, AJ42, AJ45, AJ52 and AV2S1, AV2S3, AV3S1, AV8S1, AV12S1, AV15S1, ADV21S1/DV5, AV22S1S and AV23S1, respectively. In most cases the identity of amino acid sequences between corresponding Aotus and human genes was greater than 80%. This marked conservation of TRA gene sequences indicates a close structural relationship of Aotus and human TcR and demonstrates that the TcR repertoire in primates is remarkably stable. The results support the concept of using Aotus monkeys, which are susceptible to infection with the human malaria parasite Plasmodium falciparum, as an animal model for the evaluation of molecularly defined malaria vaccine candidates.
Swiss Tropical Institute, Socinstrasse 57, CH-4002 Basel, Switzerland.
N
N
Favre
C
C
Daubenberger
J
J
Marfurt
A
A
Moreno
M
M
Patarroyo
G
G
Pluschke
D000818:Animals; D001029:Aotus trivirgatus / Q000276:immunology*; D001483:Base Sequence; D004247:DNA; D004195:Disease Models, Animal; D015332:Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; D006801:Humans; D008288:Malaria / Q000276:immunology; D008969:Molecular Sequence Data; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
D016693:Receptors, Antigen, T-Cell, alpha-beta; D004247:DNA