Sequence profile of the parallel beta helix in the pectate lyase superfamily.
The parallel beta helix structure found in the pectate lyase superfamily has been analyzed in detail. A comparative analysis of known structures has revealed a unique sequence profile, with a strong positional preference for specific amino acids oriented toward the interior of the parallel beta helix. Using the unique sequence profile, search patterns have been constructed and applied to the sequence databases to identify a subset of proteins that are likely to fold into the parallel beta helix. Of the 19 families identified, 39% are known to be carbohydrate-binding proteins, and 50% belong to a broad category of proteins with sequences containing leucine-rich repeats (LRRs). The most striking result is the sequence match between the search pattern and four contiguous segments of internalin A, a surface protein from the bacterial pathogen Listeria monocytogenes. A plausible model of the repetitive LRR sequences of internalin A has been constructed and favorable 3D-1D profile scores have been calculated. Moreover, spectroscopic features characteristic of the parallel beta helix topology in the pectate lyases are present in the circular dichroic spectrum of internalin A. Altogether, the data support the hypothesis that sequence search patterns can be used to identify proteins, including a subset of LRR proteins, that are likely to fold into the parallel beta helix.
Heffron
S
S
Department of Physiology and Biophysics, University of California, Irvine, California, 92697-4560, USA.
Moe
G R
GR
Sieber
V
V
Mengaud
J
J
Cossart
P
P
Vitali
J
J
Jurnak
F
F
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry; D002942:Circular Dichroism; D016208:Databases, Factual; D007930:Leucine / Q000737:chemistry; D008089:Listeria monocytogenes / Q000201:enzymology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D012984:Software
D001426:Bacterial Proteins; C069761:internalin protein, Bacteria; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D007930:Leucine