Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group.
Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.
Misiura
K
K
Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lódź. kmisiura@bio.cbmm.lodz.pl
Wójcik
M
M
Krakowiak
A
A
Koziołkiewicz
M
M
Pawłowska
Z
Z
Pluskota
E
E
Cierniewski
C S
CS
Stec
W J
WJ
D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D017395:Plasminogen Activator Inhibitor 1 / Q000494:pharmacology; D016914:Ribonuclease H / Q000378:metabolism; D012260:Ribonucleases / Q000097:blood; D012995:Solubility; D014320:Triphenylmethyl Compounds / Q000737:chemistry*
D008055:Lipids; D009838:Oligodeoxyribonucleotides; D016376:Oligonucleotides, Antisense; D017395:Plasminogen Activator Inhibitor 1; D014320:Triphenylmethyl Compounds; D012260:Ribonucleases; D016914:Ribonuclease H