Detection of thymine [2+2] photodimer repair in DNA: selective reaction of KMnO4.
The specific reaction of potassium permanganate with thymine in single-stranded DNA was employed to analyze thymine [2+2] dimer repair in DNA and in DNA/peptide nucleic acid hybrid duplexes. This simple and highly sensitive chemical assay is convenient for monitoring repair of thymine dimers in oligonucleotides.
Ramaiah
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D
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.
Koch
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T
Orum
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Schuster
G B
GB
D004260:DNA Repair; D004277:DNA, Single-Stranded / Q000737:chemistry; D004255:Deoxyribodipyrimidine Photo-Lyase / Q000378:metabolism*; D010777:Photochemistry; D011196:Potassium Permanganate; D011740:Pyrimidine Dimers / Q000032:analysis*; D017422:Sequence Analysis, DNA
D004277:DNA, Single-Stranded; D011740:Pyrimidine Dimers; D011196:Potassium Permanganate; D004255:Deoxyribodipyrimidine Photo-Lyase