Intradermal leukotriene B4, but not prostaglandin E2, induces itch-associated responses in mice.
The itch-associated responses induced by intradermal injection of leukotriene B4 and prostaglandin E2 were studied in mice. Leukotriene B4(0.001-1 nmol/site) elicited scratching of the injected site; the dose-response curve was bell-shaped with a peak effect at 0.03 nmol/site. The effect of leukotriene B4 (0.03 nmol/site) started within 3 min, peaked in the second 10-min period, had almost subsided by 30 min, and was inhibited by the simultaneous injection of the leukotriene B4 receptor antagonist ONO-4057, 5-[2-(2carboxyethyl)-3-(6-( p-methoxyphenyl)-5E-hexenyl) oxyphenyoxy] valeric acid. Prostaglandin E2 (0.003-300 nmol/site) did not significantly elicit scratching. The results raise the possibility that leukotriene B4 is an endogenous itch mediator in the skin.
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
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Kuraishi
D000818:Animals; D015232:Dinoprostone / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D007271:Injections, Intradermal; D007975:Leukotriene B4 / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010666:Phenylpropionates / Q000494:pharmacology; D011537:Pruritus / Q000378:metabolism*; D018102:Receptors, Leukotriene B4 / Q000037:antagonists & inhibitors
D010666:Phenylpropionates; D018102:Receptors, Leukotriene B4; C067523:ONO-LB 457; D007975:Leukotriene B4; D015232:Dinoprostone