Uptake of [3H]dopamine in isolated chromaffin cells of the mouse: modulation by intra- and extra-adrenal peptides and other secretagogues.
The effects of intra- and extra-adrenal peptides on [3H]dopamine uptake in adrenal chromaffin cells of the mouse were examined in vitro. Dopamine uptake was inhibited by acetylcholine, high potassium, reserpine, imipramine and desmethylimipramine as was in noradrenaline uptake. Among the intra-adrenal peptides, vasoactive intestinal peptide (VIP, 100 pmol/l) and neurotensin inhibited [3H]dopamine uptake by approximately 25%. Somatostatin, enkephalin, and neuropeptide Y did not cause any significant inhibition. An extra-adrenal peptide, bradykinin, inhibited the uptake while angiotensin II showed no significant effect. Intra-adrenal peptides which cause catecholamine secretion inhibit catecholamine uptake probably to extend its effect. Extra-adrenal peptide which causes catecholamine secretion also inhibits catecholamine uptake.
Department of Physiology, Niigata University School of Medicine, Japan. tetsuo@med.niigata-u.ac.jp
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Hirano
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Nagai
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Nakagawa
D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D002395:Catecholamines / Q000378:metabolism; D002469:Cell Separation; D019439:Chromaffin Cells / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism*; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009496:Neurotensin / Q000494:pharmacology; D013268:Stimulation, Chemical; D014316:Tritium; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
D002395:Catecholamines; D018765:Dopamine Uptake Inhibitors; D014316:Tritium; D000804:Angiotensin II; D014660:Vasoactive Intestinal Peptide; D009496:Neurotensin; D001920:Bradykinin; D004298:Dopamine