Expression of growth factors and their receptors in the postnatal rat cochlea.
RT-PCR was used to assay for growth factors and receptors from seven different protein families in cochlea tissues of the juvenile rat. There was a broad representation of the growth factor families in all the cochlea tissues examined, though the organ of Corti and stria vascularis expressed a greater variety than the spiral ganglion. This broad expression suggests that a variety of known growth factors play significant roles in the development, maintenance, and repair of the inner ear. The results of this survey serve as a basis for the design of future in vitro experiments that will address the ability of growth factors to protect hair cells from damage and to evoke a repair-regeneration response by injured hair cells.
Department of Human Physiology and Pathophysiology, University of Liège, Belgium. bmalgrange@ulg.ac.be
B
B
Malgrange
B
B
Rogister
P P
PP
Lefebvre
C
C
Mazy-Servais
A A
AA
Welcher
C
C
Bonnet
R Y
RY
Hsu
J M
JM
Rigo
T R
TR
Van De Water
G
G
Moonen
D000818:Animals; D001483:Base Sequence; D020934:Ciliary Neurotrophic Factor; D003051:Cochlea / Q000737:chemistry*; D017931:DNA Primers / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000032:analysis; D005346:Fibroblast Growth Factors / Q000032:analysis; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006133:Growth Substances / Q000032:analysis / Q000096:biosynthesis*; D006198:Hair Cells, Auditory / Q000254:growth & development; D008969:Molecular Sequence Data; D009414:Nerve Growth Factors / Q000032:analysis; D009419:Nerve Tissue Proteins / Q000032:analysis; D009475:Neurons, Afferent / Q000737:chemistry; D010982:Platelet-Derived Growth Factor / Q000032:analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017978:Receptors, Growth Factor / Q000032:analysis / Q000096:biosynthesis*; D013002:Somatomedins / Q000032:analysis; D013136:Spiral Ganglion / Q000737:chemistry; D019089:Stem Cell Factor / Q000032:analysis; D013316:Stria Vascularis / Q000737:chemistry
D020934:Ciliary Neurotrophic Factor; D017931:DNA Primers; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D006133:Growth Substances; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D010982:Platelet-Derived Growth Factor; D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D013002:Somatomedins; D019089:Stem Cell Factor; D005346:Fibroblast Growth Factors; D004815:Epidermal Growth Factor