A novel technique for the isolation of Lewy bodies in brain.
Previously, immunohistochemical methods were primarily used to detect and provide indirect evidence on the composition of Lewy bodies, the pathological hallmark of Parkinson's disease. This was chiefly because there are very few procedures that describe the isolation of these structures. We report here a relatively simple method that we have developed for the exclusive isolation of Lewy bodies from brain tissue. The isolation of the Lewy bodies and subsequent evaluation of their components may furnish an insight into their role in the neurodegenerative mechanism(s) operating in the spectrum of Lewy body disorders.
Department of Psychiatry, University of Würzburg, Germany.
J
J
Sian
R
R
Hensiek
D
D
Senitz
G
G
Muench
K
K
Jellinger
P
P
Riederer
M
M
Gerlach
D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal; D001921:Brain / Q000473:pathology* / Q000648:ultrastructure; D002540:Cerebral Cortex / Q000276:immunology / Q000648:ultrastructure; D003584:Cytological Techniques; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016631:Lewy Bodies / Q000648:ultrastructure*; D008578:Meninges / Q000473:pathology / Q000648:ultrastructure; D009410:Nerve Degeneration / Q000473:pathology
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G