[Effect of a biologically active interferon fragment on the structure of the synthetic protein carrier].
A biologically active de novo protein albeferon was studied by physical techniques including CD spectroscopy in far and near ultraviolet regions and microcalorimetry. Albeferon was obtained by grafting an active octapeptide interferon fragment into a de novo protein albebetin used as a carrier. It was shown that attachment of the octapeptide into its molecule did not weaken albebetin but even slightly improved its structure and stability. The obtained results can be used for de novo protein design and improvement.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
I Iu
IIu
Afasizheva
D A
DA
Dolgikh
Z Kh
ZKh
Abdullaev
R F
RF
Latypov
E I
EI
Tiktopulo
V N
VN
Uverskiĭ
O B
OB
Ptitsyn
M P
MP
Kirpichnikov
D006801:Humans; D007372:Interferons / Q000737:chemistry*; D010446:Peptide Fragments; D011487:Protein Conformation; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*
D010446:Peptide Fragments; D011506:Proteins; D011993:Recombinant Fusion Proteins; C074146:albebetin; D007372:Interferons