Reducing sugars trigger delta-aminolevulinic dehydratase inactivation: evidence of in vitro aspirin prevention.
1. The effect of in vitro glycation on delta-aminolevulinic dehydratase (ALA-D) under several experimental conditions was investigated. When preincubated with 500 mM glucose at 37 degrees C for 20 hr, ALA-D was 80% inactivated and glycated hemoglobin levels were increased more than fourfold. 2. Thiobarbituric acid species were not modified during glycation; therefore ALA-D inactivation cannot be attributed to glucose autoxidation. 3. Acetyl salicylic acid was effective in preventing both hemoglobin glycation and ALA-D inactivation by glucose. 4. A method has been developed for measuring protein glycation in vitro, in a crude preparation of red blood cells, which can also be applied to sugars other than glucose.
Centro de Investigaciones sobre Porfirinas y Porfirias, (CONICET-FCEN, UBA), Cuidad Universitaria, Buenos Aires, Argentina.
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Caballero
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Gerez
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Vazquez
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Batlle
D001241:Aspirin / Q000494:pharmacology*; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006442:Glycated Hemoglobin / Q000378:metabolism; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects; D000623:Porphobilinogen Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors
D001241:Aspirin; D002241:Carbohydrates; D005947:Glucose; D006442:Glycated Hemoglobin; D000623:Porphobilinogen Synthase