Design and optimization of the variables in the adsorptive stripping voltammetric determination of rufloxacin in tablets, human plasma and urine.
An adsorptive stripping voltammetric method with a hanging mercury drop electrode was developed for the determination of the fluoroquinolone rufloxacin in tablets, human plasma and urine. Measurements were obtained in differential pulse mode and a multivariate strategy was used to optimize the variables involved. Besides the independent effects of the variables, a strong interaction between scan rate and pulse duration has been found. Rufloxacin was analysed at concentrations between 1.7 x 10(-8) and 1.9 x 10(-7) M with a detection limit of 9.2 x 10(-9) M. Diluted tablet solutions and urine samples were analysed directly, while plasma samples needed an extraction procedure before voltammetric analysis. An improved HPLC procedure was used as comparative method.
Furlanetto
S
S
Dipartimento di Scienze Farmaceutiche, Università di Firenze, Italy.
Gratteri
P
P
Pinzauti
S
S
Leardi
R
R
Dreassi
E
E
Santoni
G
G
D000042:Absorption; D000890:Anti-Infective Agents / Q000032:analysis* / Q000097:blood / Q000652:urine; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D024841:Fluoroquinolones; D006801:Humans; D015999:Multivariate Analysis; D011048:Polarography; D015363:Quinolones / Q000032:analysis* / Q000097:blood / Q000652:urine; D012044:Regression Analysis; D013607:Tablets
D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D015363:Quinolones; D013607:Tablets; C060328:rufloxacin