Functional mimicry: elicitation of a monoclonal anti-idiotypic antibody hydrolizing beta-lactams.
Antigen mimicry by anti-idiotypic antibodies is investigated as a reliable strategy to achieve molecular imprinting of an enzymatic activity. A monoclonal anti-idiotypic antibody (Ab2-9G4H9) was elicited by using a monoclonal antibody (Ab1-7AF9) specific for the beta-lactamase active site. Catalytic features of Ab2 were characterized with beta-lactamase substrates. The antibody combining site appeared to have retained a part of the catalytic specificity. The relevance of the idiotypic mimicry concept for the generation of catalytic antibodies was further demonstrated by eliciting a third generation antibody (Ab3), which was shown to recognize beta-lactamase: the complete internal image properties of Ab2 9G4H9, including binding and catalytic properties, were thus checked.
Avalle
B
B
Laboratoire de Technologie Enzymatique-UPRES-A CNRS 6022, Université de Technologie de Compiègne, France.
Thomas
D
D
Friboulet
A
A
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D002384:Catalysis; D051379:Mice; D018716:Molecular Mimicry; D015394:Molecular Structure; D001618:beta-Lactamases / Q000276:immunology*; D047090:beta-Lactams / Q000378:metabolism*
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D047090:beta-Lactams; D001618:beta-Lactamases