Sequence selective interaction between nucleotides and intercalators bound to water soluble dextran: an application to the affinity chromatography of dinucleotides.
The analysis of association constant between dextran coupled intercalators and nucleotides revealed the base- and sequence-selective affinity to mono- and dinucleotides in aqueous solution. Acridine bound CH-Sepharose 4B, designed as the affinity stationary phase for nucleotides, also showed base- and sequence-selective affinity.
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan. shirai@imcbns.iam.u-tokyo.ac.jp
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Shirai
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Ito
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Iwasaki
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Hashimoto
D000873:Anthracenes / Q000737:chemistry; D002240:Carbohydrate Sequence; D002846:Chromatography, Affinity; D003911:Dextrans / Q000737:chemistry*; D005449:Fluorenes / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry*; D008024:Ligands; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry; D011083:Polycyclic Compounds / Q000737:chemistry; D012265:Ribonucleotides / Q000737:chemistry*
D000873:Anthracenes; D003911:Dextrans; D005449:Fluorenes; D007364:Intercalating Agents; D008024:Ligands; D009841:Oligonucleotides; D011083:Polycyclic Compounds; D012265:Ribonucleotides