A comparative genetic analysis of the Irish greyhound population using multilocus DNA fingerprinting, canine single locus minisatellites and canine microsatellites.
Pairwise analysis of HinfI/33.6 DNA fingerprints from a total of one hundred and fifty-three Irish greyhounds of known pedigree were used to determine band-share estimates of unrelated, first-degree and second-degree relationships. Forty-eight unrelated Irish greyhounds were used to determine allele frequencies for three single-locus minisatellites, and following a preliminary screen, eight of the most polymorphic tetra-nucleotide microsatellites from a panel of 15. The results indicated that both band-share estimates by DNA fingerprinting and microsatellite allele frequencies are highly effective in resolving parentage in this greyhound population, while single-locus minisatellites showed limited polymorphism and could not be used alone for routine parentage testing in this breed. The present study also demonstrated that, to obtain optimal resolution of parentage, sample sets of known pedigree status are required to determine the band-share distribution and/or microsatellite allele frequencies.
Sutton
M D
MD
Zoology Department, University College Dublin, Belfield, Republic of Ireland.
Holmes
N G
NG
Brennan
F B
FB
Binns
M M
MM
Kelly
E P
EP
Duke
E J
EJ
D000818:Animals; D015139:Blotting, Southern; D004247:DNA / Q000097:blood / Q000302:isolation & purification; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D004276:DNA, Satellite / Q000235:genetics*; D004285:Dogs / Q000235:genetics*; D005787:Gene Frequency; D006579:Heterozygote; D007494:Ireland; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity
D004276:DNA, Satellite; D004247:DNA