[Conformational analysis of complexes of RNA polymerase from Escherichia coli with DNA].
Conformational behaviour of T2 DNA during its complex formation with E.coli RNA polymerase was studied by spin label technique. T2 DNA was selectively modified at its readily melting AT-rich sites by bromoacetooxypiperidine-1-oxyl-radical. Specific conformational changes are induced in DNA structure by RNA polymerase attachment. The changes are observed under the conditions when open transcriptionally competent complexes are formed. The readily melting sites of T2 DNA were shown to be involved in the formation of functionally active promoters.
Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
S G
SG
Kamzolova
N N
NN
Ivanova
S S
SS
Kamzalov
L V
LV
Iakushevich
D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli; D017900:Myoviridae; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011487:Protein Conformation; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases