The value of interphase fluorescence in situ hybridization in the study of patients with lymphoproliferative disorders: further evidence for a higher sensitivity of detecting chromosomes 7 and 8 aneuploidy.
Dual-color interphase fluorescence in situ hybridization (I-FISH) for chromosomes 7 and 8 was studied retrospectively on 32 patients with suspected lymphoid disorders, and the results were compared with standard cytogenetics. One of 29 (3.4%) patients with lymphoid malignancy showed cytogenetically detectable aneuploidy for chromosomes 7 and 8. In an additional 5 patients (17.2%), I-FISH unmasked chromosomal loss and gain that were not detected by standard metaphase analysis. This represents 19% of the 21 studied patients with acute lymphoblastic leukemia (ALL). These findings indicate that aneuploidies for chromosomes 7 and 8 are underreported in ALL and further demonstrate higher sensitivity of I-FISH for detecting numerical chromosomal rearrangements in leukemic cells.
Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029-6574, USA.
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Lempert
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Kafko
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Scalise
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Najfeld
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D001853:Bone Marrow / Q000502:physiology; D002503:Centromere / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D002897:Chromosomes, Human, Pair 7; D002898:Chromosomes, Human, Pair 8; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007223:Infant; D008297:Male; D008875:Middle Aged; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D012680:Sensitivity and Specificity