Monoclonal antibodies to P-glycoprotein: preparation and applications to basic and clinical research.
P-Glycoprotein, a member of the ABC transporter group, plays an important role in anticancer drug resistance. Many monoclonal antibodies to P-glycoprotein have been developed and used to study the molecular and cellular functions of P-glycoprotein. These monoclonal antibodies were used for gene cloning, purification and reconstitution of P-glycoprotein, analysis of transport functions of P-glycoprotein, immunological detection and selective killing of tumors that express P-glycoprotein for diagnostic, and therapeutic purposes.
Naito
M
M
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
Tsuruo
T
T
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis* / Q000096:biosynthesis / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000633:toxicity; D002470:Cell Survival; D003001:Cloning, Molecular / Q000379:methods; D018432:Drug Resistance, Multiple; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000302:isolation & purification; D007158:Immunologic Techniques; D051379:Mice; D008807:Mice, Inbred BALB C
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D000939:Epitopes; D007074:Immunoglobulin G