[Effect of surfactants on peroxidase activity. II. Effect of cationic surfactants].
The effect of various cationic surfactants on the initial rate of the 5-aminosalicylic acid peroxidation with horseradish peroxidase was studied. With increasing concentration of the surfactant, the rate first increased and then decreased. We attributed these changes to a primary activation of the enzyme and/or an increase in the concentration of the substrate in the region of the active site and to the subsequent inactivation of the protein with surfactant followed by its denaturing, respectively.
Skryabin State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia.
A I
AI
Davletshin
N A
NA
Kalabina
S Iu
SIu
Zaĭtsev
V V
VV
Egorov
D002412:Cations; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019804:Mesalamine / Q000737:chemistry; D008823:Micelles; D010084:Oxidation-Reduction; D011489:Protein Denaturation; D013329:Structure-Activity Relationship; D013501:Surface-Active Agents / Q000737:chemistry*
D002412:Cations; D004791:Enzyme Inhibitors; D008823:Micelles; D013501:Surface-Active Agents; D019804:Mesalamine; D006735:Horseradish Peroxidase