Flow modulates endothelial regulation of smooth muscle cell proliferation: a new model.
BACKGROUND
With a co-culture model, we have previously demonstrated that endothelial cells (ECs) exert regulatory control over smooth muscle cell (SMC) behavior. ECs appeared to stimulate SMC proliferation in static culture. This study was performed to test the hypothesis that the EC stimulation of SMC proliferation was effected by shear stress.


METHODS
Bovine SMCs were cultured on a thin semipermeable membrane either alone or opposite ECs in co-culture (SMC/EC). A novel parallel-plate flow device was developed and used for exposing the EC side of the co-culture to shear stress. EC and SMC proliferation rates were determined after 24 hours' exposure to 0, 1, or 10 dynes/cm2 of shear stress.


RESULTS
SMC proliferation decreased significantly from 362 +/- 65 cpm/microgram DNA (control, mean +/- SEM) to 68 +/- 43 cpm/microgram (1 dyne/cm2) and 99 +/- 18 cpm/microgram (10 dynes/cm2)(P < .05). EC proliferation after flow decreased as compared with no-flow controls 71 +/- 15 cpm/micrograms DNA (control, mean +/- SEM) to 29 +/- 5 cpm/microgram (1 dyne/cm2) and 21 +/- 4 cpm/microgram (10 dynes/cm2)(P < .05).


CONCLUSIONS
In a model designed to study SMC/EC interactions in a flow environment, it was seen that EC exposure to shear stress alters the growth characteristics of SMCs. This suggests that hemodynamic mechanical forces may be sufficient to alter the EC regulation of SMC behavior.
Department of Surgery, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, New Brunswick 08903-0019, USA.
G B
GB
Nackman
M F
MF
Fillinger
R
R
Shafritz
T
T
Wei
A M
AM
Graham
D000818:Animals; D002417:Cattle; D002450:Cell Communication / Q000502:physiology*; D002455:Cell Division / Q000502:physiology; D015194:Diffusion Chambers, Culture / Q000295:instrumentation / Q000379:methods; D004730:Endothelium, Vascular / Q000166:cytology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010477:Perfusion; D013314:Stress, Mechanical