Ultrastructure of oogenesis in Penaeus kerathurus (Crustacea, Decapoda). I. Previtellogenic oocytes.
Although Malacostracan species represent an important alimentary human resource, the ultrastructure of oogenesis in P. kerathurus remains unknown. Previtellogenic oocytes of Penaeus kerathurus possess a large nucleus with several peripheral nucleoli. The endoplasmic reticulum (ER) is originated from expansions of the nuclear envelope (NE) and contains small dense granules, which are first formed inside the intermembranous space of the NE but are later exported to the ER lumen. Direct vesiculation from the NE and ER then give rise to the Golgi complexes. Small yolk vesicles appear to be mainly formed by vesiculation of the ER, but also receive materials from the Golgi complexes. They contain a fine fibrillar content which seems to originate from decondensation of the small dense granules. Small vesicles and small multivesicular bodies originated from the NE, ER and Golgi complexes, as also myelin figures directly shedded from the NE, fuse together to give origin to large multivesticular bodies (MVB). These organelles, which have an incomplete membrane and appear meshed within nuage materials, give origin, at a later stage, to lipid droplets that are thereafter extruded into the cytoplasm. Neighbouring oocytes exhibit intercellular bridges, the remaining of their surface being surrounded by a single layer of flattened follicular cells. These results show for the first time in Malacostraca the existence of oocyte intercellular bridges, that the ER and Golgi complexes arise from NE activity, that early yolk formation is endogenous and derives from the activity of the NE, ER and Golgi complexes, and that lipid droplets are products of intracellular membrane recycling activity occurring within large multivesicular bodies.
Carvalho
F
F
CIMAR, Laboratories of Physiology, University of Porto, Portugal. msousa@icbas.up.pt
Sousa
M
M
Oliveira
E
E
Carvalheiro
J
J
Baldaia
L
L
D000818:Animals; D003445:Crustacea; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005260:Female; D006056:Golgi Apparatus / Q000648:ultrastructure; D006801:Humans; D009685:Nuclear Envelope / Q000648:ultrastructure; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure*; D009866:Oogenesis; D015388:Organelles / Q000648:ultrastructure*; D010053:Ovary / Q000502:physiology / Q000648:ultrastructure; D014817:Vitelline Membrane