Ascidian mitochondrial tRNA(Met) possessing unique structural characteristics.
Methionine tRNA was purified from muscle mitochondria of the ascidian Halocynthia roretzi and its RNA sequence was determined. Analysis of the nucleotide sequence revealed that unlike most metazoan mitochondrial tRNAs(Met), which have a highly conserved cytidine (C) or C-derivative at the wobble position, the H. roretzi mitochondrial tRNA(Met) possesses 5-carboxymethylaminomethyluridine (cmnm5U) at the first position of the anticodon. This is the first report of a single mitochondrial tRNA(Met) species having uridine (U) or a U-derivative at the wobble position.
Kondow
A
A
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Japan.
Yokobori
S
S
Ueda
T
T
Watanabe
K
K
D000818:Animals; D000926:Anticodon / Q000235:genetics; D001483:Base Sequence; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000737:chemistry*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis; D012313:RNA / Q000737:chemistry*; D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met / Q000737:chemistry*; D017423:Sequence Analysis, RNA; D014529:Uridine / Q000031:analogs & derivatives; D014561:Urochordata
D000926:Anticodon; D009711:Nucleotides; D000077278:RNA, Mitochondrial; D012358:RNA, Transfer, Met; D012313:RNA; D008718:Methionine-tRNA Ligase; D014529:Uridine