Characterization of the novel, pediatric Hodgkin disease-derived cell line HKB-1.
BACKGROUND
A novel Hodgkin disease (HD)-derived cell line, designated HKB-1, was established from pulmonary HD of nodular sclerosing subtype of a 14-year-old-girl.


RESULTS
Immunophenotypically, HKB-1 cells are of B cell in phenotype, being also positive for markers CD15, CD25 and CD30. Transformation of the cell line by Epstein-Barr virus (EBV) was excluded by failure to detect EBV antigens and EBV DNA in cultured cells. Chromosome studies of HKB-1 showed a pseudodiploid karyotype with complex clonal structural aberrations. The detection of a monoclonal immunoglobulin heavy chain (IgH) gene rearrangement confirmed the derivation of the HKB-1 from the B cell lineage and its monoclonality. HKB-1 produced high amounts of interleukin (IL)-6 as detected in culture supernatant whereas no secretion of IL-1 beta, IL-2, IL-4, IL-10, IL-12 or interferon (IFN)-gamma was detected.


CONCLUSIONS
Our studies indicate that this cell line is of tumor origin.
Department of Pediatrics, Medical University of Lübeck, Germany.
H J
HJ
Wagner
F
F
Klintworth
W
W
Jabs
K
K
Lange
B
B
Schlegelberger
L
L
Harder
A C
AC
Feller
P
P
Bucsky
D000293:Adolescent; D003131:Combined Modality Therapy; D016207:Cytokines / Q000378:metabolism; D005260:Female; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006689:Hodgkin Disease / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000628:therapy / Q000821:virology; D006801:Humans; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism / Q000473:pathology* / Q000628:therapy / Q000821:virology; D009363:Neoplasm Proteins / Q000378:metabolism; D010641:Phenotype; D014407:Tumor Cells, Cultured / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D016207:Cytokines; D009363:Neoplasm Proteins