Immunohistochemical localization of galectin-3 in malignant and benign human thyroid tissue.
BACKGROUND
Galectin-3 is an endogenous beta-galactoside binding lectin with putative roles in development, immunomodulation, transformation and metastasis. The purpose of this study was to analyze galectin-3 expression in a series of human thyroid neoplastic lesions.


METHODS
A total of 76 cases, including 47 specimens of thyroid malignancies, 14 follicular adenomas and 15 specimens of normal thyroid tissue, were analyzed immunohistochemically using a monoclonal antibody to galectin-3 and avidin-biotin-peroxidase complex (ABC) method.


RESULTS
The immunohistochemical staining results showed galectin-3 expression in neoplastic cells of all 20 cases of papillary carcinoma, 11 out of 15 follicular carcinomas, both oxyphilic carcinomas, all 10 anaplastic carcinomas and 5 out of 14 follicular adenomas. Galectin-3 localization was mostly cytoplasmic, but also membraneous or nuclear in some cells. Follicular cells in normal thyroid tissue were negative.


CONCLUSIONS
The results of this study indicate that galectin-3 gene is expressed at the protein level in most thyroid carcinomas and some adenomas. Galectin-3 expression was not clearly correlated with histopathological aggressiveness, dedifferentiation state or determination of malignancy of the follicular tumour. The role of galectin-3 in thyroid tumour biology remains to be elucidated.
Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Yugoslavia. dubravka@inep.co.yu
D
D
Cvejic
S
S
Savin
I
I
Paunovic
S
S
Tatic
M
M
Havelka
J
J
Sinadinovic
D000236:Adenoma / Q000473:pathology*; D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation / Q000032:analysis*; D002277:Carcinoma / Q000473:pathology; D002291:Carcinoma, Papillary / Q000473:pathology; D037502:Galectin 3; D006801:Humans; D007150:Immunohistochemistry; D037102:Lectins / Q000032:analysis; D013961:Thyroid Gland / Q000166:cytology* / Q000473:pathology; D013964:Thyroid Neoplasms / Q000473:pathology*
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D037502:Galectin 3; D037102:Lectins