The impact of two-hybrid and related methods on biotechnology.
Two-hybrid technology has contributed significantly to the unraveling of molecular regulatory networks by facilitating the discovery of protein interactions. Outgrowths of these methods are developing rapidly, including interaction mating to identify false positives and map protein networks, two-bait systems, systems not based on transcription, and systems permitting the selection of peptide aptamers to manipulate gene and allele function. These advances promise to have a significant impact on industrial biotechnology and drug development.
Colas
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P
Laboratoire de Biologie Moléculaire et Cellulaire, UMR49 CNRS/Ecole Normale Supérieure de Lyon, France. Pierre.Colas@ens-lyon.fr
Brent
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D001709:Biotechnology / Q000639:trends*; D004268:DNA-Binding Proteins / Q000235:genetics*; D017930:Genes, Reporter; D005818:Genetic Engineering / Q000639:trends*; D006801:Humans; D011485:Protein Binding
D004268:DNA-Binding Proteins