Rat carnitine palmitoyltransferase Ibeta mRNA splicing isoforms.
Carnitine palmitoyltransferase I (CPT-I) catalyzes the rate-determining step in mitochondrial fatty acid beta-oxidation. The enzyme has two cognate structural genes (alpha and beta) that are differentially expressed in tissues. We show multiple mature mRNAs in rat heart derived from alternative splicing of CPT-Ibeta transcripts. Two novel messages are deleted for regions of the previously described mRNA that encode membrane-spanning and regulatory domains, suggesting that the cognate isozymes will exhibit unique kinetic characteristics.
Yu
G S
GS
Diabetes Unit and Medical Services, Massachusetts General Hospital, 149 Thirteenth Street, MGH East, CNY 149 8218, Charlestown, MA 02129, USA.
Lu
Y C
YC
Gulick
T
T
D000818:Animals; D002334:Carnitine O-Palmitoyltransferase / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics*; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D015347:RNA Probes; D012326:RNA Splicing; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D051381:Rats; D016415:Sequence Alignment
D007527:Isoenzymes; D015347:RNA Probes; D012333:RNA, Messenger; D002334:Carnitine O-Palmitoyltransferase