Cloning, expression, purification of rat tumor necrosis factor and demonstration of its antitumor activity in Bomirski amelanotic melanoma.
In order to analyze the effect of tumor necrosis factor (TNF) on the growth of Bomirski melanomas of hamster, we cloned, expressed and purified to homogeneity the rat tumor necrosis factor. The detailed procedure of chromatographic purification of the cytokine is reported. Polyclonal antibodies generated against the recombinant rat preparation were able to neutralize the rat and hamster TNF. We have found that the preparation of rat TNF is active in vivo and inhibits the growth of a Bomirski (Ab) amelanotic melanoma. Thus, a novel model system has been developed, which should facilitate analysis of antitumor and immunomodulatory function of TNF in cancer hosts.
Koszałka
P
P
Department of Histology and Immunology, Medical University of Gdańsk, Poland.
Breza
H
H
Bigda
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D002845:Chromatography; D002850:Chromatography, Gel; D003001:Cloning, Molecular; D006224:Cricetinae; D004354:Drug Screening Assays, Antitumor; D018328:Melanoma, Amelanotic / Q000188:drug therapy*; D008647:Mesocricetus; D009368:Neoplasm Transplantation; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000627:therapeutic use; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use*
D000906:Antibodies; D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha