Different genome organization in two new cell lines established from human gastric carcinoma.
Two gastric cancer cell lines, AKG and GK2, were established from a pleural and an ascitic effusion, respectively. GK2 cells have a pseudodiploid karyotype with an add(6)(q27) chromosome in all metaphases examined. The karyotype of AKG cells is highly rearranged: FISH analysis with painting probes has shown that DNA sequences derived from single chromosomes are scattered on several (as many as eight) markers. In this cell line, the C-MYC and the K-RAS oncogenes are amplified. The organization and the copy number of the C-MYC-amplified units are different from the K-RAS units, suggesting that the two oncogenes were amplified independently. The presence of a few marker chromosomes carrying both C-MYC and K-RAS could be due to translocation events that followed the amplification.
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
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Bertoni
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Mucciolo
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Ricotti
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Amadori
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Giulotto
D000818:Animals; D015197:Carcinogenicity Tests; D002277:Carcinoma / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D002455:Cell Division; D002869:Chromosome Aberrations; D019008:Drug Resistance, Neoplasm; D005260:Female; D018628:Gene Dosage; D015321:Gene Rearrangement; D016259:Genes, myc; D011905:Genes, ras; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods; D007621:Karyotyping; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008819:Mice, Nude; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology*; D014407:Tumor Cells, Cultured