Detection of GB virus C by the RT-PCR LCx system.
The recent publication of representative genomic sequences of GBV-C has permitted the selection of PCR primers for detection of GBV-C in clinical samples by PCR techniques. Traditional amplification methodologies which couple reverse transcription polymerase chain reaction (RT-PCR) and Southern blot detection are slow, cumbersome, and can be technique dependent. This has hampered studies to determine the clinical significance of GBV-C. We report the selection of highly conserved PCR primers and a probe useful for semi-automated RT-PCR using the Abbott LCx system. This adaptation of the LCx system expands its capabilities to include the detection of RNA by RT-PCR, in addition to DNA detection by ligase chain reaction (LCR).
Marshall
R L
RL
Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064, USA.
Cockerill
J
J
Friedman
P
P
Hayden
M
M
Hodges
S
S
Holas
C
C
Jennings
C
C
Jou
C K
CK
Kratochvil
J
J
Laffler
T
T
Lewis
N
N
Scheffel
C
C
Traylor
D
D
Wang
L
L
Solomon
N
N
D001331:Automation; D015139:Blotting, Southern; D017931:DNA Primers; D015342:DNA Probes; D004866:Equipment Contamination; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000453:epidemiology; D006801:Humans; D007124:Immunoenzyme Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood* / Q000235:genetics; D015203:Reproducibility of Results; D018401:Sample Size; D012680:Sensitivity and Specificity
D017931:DNA Primers; D015342:DNA Probes; D012367:RNA, Viral