[A simple method for testing basic cytotoxicity in vitro].
The authors have recommended a simple method for further trial, of in vitro cytotoxicity test, for screening examinations, in order to complete in vivo methods or use it as an alternative technique. Briefly, it is as follows: the substance studied is treated in bidistilled water with the suspension of a well-known living cells of Candida strain, and then the changing of the number of surviving cells is followed up daily. The result is expressed as a percentage relation of the initial number of living cells, in survival curves in comparison with the controls. The number of surviving cells is inversely proportional to the toxicity of the substance studied. The method is very simple and cheap.
Disciplina de Microbiologie, U.M.F., Tg. Mureş.
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D002176:Candida albicans / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods* / Q000706:statistics & numerical data; D013997:Time Factors