Model for inactivation of alpha-amylase in the presence of salts: theoretical and experimental studies.
According to a previous report, only the smaller anions, like chlorides,that readily fit into the anion binding site of alpha-amylase can cause an increased stability (relative to enzymes in aqueous solution), and the anions that are too large to fit into the binding site should have no effect on the enzyme. Even though the results on large benzoate ions are consistent with the above postulate, much larger citrate ions from sodium and potassium citrate show stabilization at moderate salt concentrations and follow an expected trend of low stability only at large salt concentrations. The citrate ions from ammonium citrate exhibit very little to almost no stabilization. In addition, low to moderate concentrations of NaCl that provide a large stability to the enzyme show almost no stability in the presence of EDTA. We put forward an inactivation model that involves a reversible dissociation of the anion bound to the protein, followed by a reversible inactivation step of calcium ion dissociation and an irreversible denaturation step of apoenzyme.
Chemistry Department, The Pennsylvania State University, DuBois, Pennsylvania 15801, USA.
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Lecker
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Khan
D000838:Anions / Q000378:metabolism; D001565:Benzoates / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism; D004492:Edetic Acid / Q000378:metabolism / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D008956:Models, Chemical; D011188:Potassium / Q000378:metabolism; D011485:Protein Binding; D011489:Protein Denaturation / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D012492:Salts / Q000378:metabolism / Q000494:pharmacology*; D012965:Sodium Chloride / Q000378:metabolism / Q000494:pharmacology; D000516:alpha-Amylases / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*
D000838:Anions; D001565:Benzoates; D002712:Chlorides; D000644:Quaternary Ammonium Compounds; D012492:Salts; D019343:Citric Acid; D012965:Sodium Chloride; D004492:Edetic Acid; D000516:alpha-Amylases; D011188:Potassium