Use of deuterium oxide to measure de novo fatty acid synthesis in normal subjects consuming different dietary fatty acid composition1.
The effect of dietary linoleic (C18:2n-6) and palmitic acids (C16:0) on rate of hepatic de novo fatty acid synthesis was assessed in normal subjects. The diet was formulated to provide combinations of high and low levels of C18:2n-6 and C16:0. After 21 days of diet treatment, plasma triacylglycerol level and incorporation of deuterium into the plasma very low density lipoprotein triacylglycerol (VLDL-TG) pool over 24 hours was measured. Plasma triacylglycerol levels were within the normal range. Increasing dietary intake of linoleic acid decreased plasma triacylglycerol level when subjects consumed a low level of dietary palmitic acid. The relative and net amount of de novo synthesized fatty acid in the plasma VLDL-TG pool was not influenced by the diet treatments. A relationship between plasma triacylglycerol level and rate of hepatic de novo fatty acid synthesis was observed.
Konrad
S D
SD
Nutrition and Metabolism Research Group, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Cook
S L
SL
Goh
Y K
YK
French
M A
MA
Clandinin
M T
MT
D000328:Adult; D017666:Deuterium Oxide; D004041:Dietary Fats / Q000008:administration & dosage* / Q000032:analysis; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000008:administration & dosage / Q000032:analysis; D008099:Liver / Q000378:metabolism*; D008297:Male; D019308:Palmitic Acid / Q000008:administration & dosage / Q000032:analysis; D014280:Triglycerides / Q000032:analysis* / Q000097:blood
D004041:Dietary Fats; D005227:Fatty Acids; D014280:Triglycerides; D019308:Palmitic Acid; D019787:Linoleic Acid; D017666:Deuterium Oxide