Exercise training reduces myocardial lipid peroxidation following short-term ischemia-reperfusion.
OBJECTIVE
The purpose of these experiments was to test the hypothesis that endurance exercise training will reduce myocardial lipid peroxidation following short-term ischemia and reperfusion (I-R).


METHODS
Female Sprague-Dawley rats (4 months old) were randomly assigned to either a sedentary control group (N = 13) or to an exercise training group (N = 13). The exercise trained animals ran 4 d.wk-1 (90 min.d-1) at approximately 75% V02max. Following a 10-wk training program, animals were anesthetized, mechanically ventilated, and the chest was opened by thoracotomy. Coronary occlusion was achieved by a ligature around the left coronary artery; occlusion was maintained for 5 min followed by a 10-min period of reperfusion.


RESULTS
Although training did not alter (P > 0.05) myocardial activities of antioxidant enzymes (superoxide dismutase and glutathione peroxidase), training was associated with significant increase (P > 0.05) in heat shock protein (HSP72) in the left ventricle. Compared with controls, trained animals exhibited significantly lower levels (P < 0.05) of myocardial lipid peroxidation following I-R.


CONCLUSIONS
These data support the hypothesis that exercise training provides protection against myocardial lipid peroxidation induced by short-term I-R in vivo.
Demirel
H A
HA
Department of Exercise and Sport Sciences and Physiology, University of Florida, Gainesville, USA.
Powers
S K
SK
Caillaud
C
C
Coombes
J S
JS
Naito
H
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Fletcher
L A
LA
Vrabas
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Jessup
J V
JV
Ji
L L
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D000818:Animals; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000097:blood; D015227:Lipid Peroxidation; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood
D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase