Faecal short-chain fatty acids in patients with antibiotic-associated diarrhoea, before and after faecal enema treatment.
BACKGROUND
Antibiotic-associated diarrhoea (AAD) may range from mild disturbances to severe pseudomembranous colitis. Many antibiotics affect several intestinal microflora-associated characteristics, such as short-chain fatty acid (SCFA) pattern. In the present study we investigated SCFAs in 31 patients on admittance to the hospital for severe AAD. Nine patients were followed up more extensively after they had received an enema containing faecal microflora from a healthy person on a Western diet.


METHODS
Faecal SCFAs were determined by gas chromatography. The enema was characterized before use.


RESULTS
AAD patients showed significant disturbances in faecal SCFA pattern. Clinically, most enema-treated patients recovered within days and had no relapses within 18 months.


CONCLUSIONS
Intestinal microflora showed great disturbances, and the amounts of SCFAs were reduced, although the diarrhoea was not related to total amount SCFAs. Administration of a faecal enema resulted in the clinical recovery of most patients with severe diarrhoea within 4 days.
Dept. of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden.
A
A
Gustafsson
S
S
Lund-Tønnesen
A
A
Berstad
T
T
Midtvedt
E
E
Norin
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000900:Anti-Bacterial Agents / Q000009:adverse effects* / Q000627:therapeutic use; D003967:Diarrhea / Q000139:chemically induced* / Q000503:physiopathology / Q000628:therapy*; D004733:Enema / Q000379:methods*; D005232:Fatty Acids, Volatile / Q000032:analysis*; D005243:Feces / Q000737:chemistry* / Q000382:microbiology; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000382:microbiology; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D012044:Regression Analysis; D012720:Severity of Illness Index; D016896:Treatment Outcome
D000900:Anti-Bacterial Agents; D005232:Fatty Acids, Volatile