Genomic organization and chromosomal localization of the human SGK protein kinase gene.
The SGK protein kinase is a novel member of the serine/threonine protein kinase family. Its corresponding gene belongs to the group of immediate-early genes. SGK transcription is controlled by cell volume alterations in different cell lines. To analyze the genomic structure and chromosomal location of the SGK gene, a human P1 clone was isolated by screening a human genomic library with a SGK cDNA probe. This clone was confirmed to encode the authentic SGK gene by the detection of exon-intron structures and the correspondence between the nucleotide sequences of exons and human cDNA. Using this P1 clone as a probe for fluorescence in situ hybridization, a single chromosomal locus for SGK was assigned to band 6q23, a region frequently affected by deletion in various human neoplasms.
Waldegger
S
S
Department of Physiology I, University of Tübingen, Germany. siegfried.waldegger@uni-tuebingen.de
Erdel
M
M
Nagl
U O
UO
Barth
P
P
Raber
G
G
Steuer
S
S
Utermann
G
G
Paulmichl
M
M
Lang
F
F
D002460:Cell Line; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D003001:Cloning, Molecular; D018628:Gene Dosage; D005796:Genes / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins; D008297:Male; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D020161:Physical Chromosome Mapping; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D000099184:Serum-Glucocorticoid Regulated Kinases
D017874:Immediate-Early Proteins; D009687:Nuclear Proteins; D017346:Protein Serine-Threonine Kinases; D000099184:Serum-Glucocorticoid Regulated Kinases