Effect of nonsteroidal anti-inflammatory drugs on lipid peroxidation by hydroxyl radical.
1. Effects of acetaminophen and sodium salicylate on hydoxyl radical elimination were studied using an electron-spin-resonance spin-trapping method. The effects of these agents on lipid peroxidation of the human erythrocyte membrane were also investigated by the thiobarbituric acid method. 2. Acetaminophen and sodium salicylate depressed hydroxyl radical generated by Cu2+/H2O2. 3. Acetaminophen inhibited Cu2+/H2O2-dependent lipid peroxidation; however, sodium salicylate enhanced Cu2+/H2O2-dependent lipid peroxidation.
Tsujimoto
Y
Y
Department of Endodontics, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Saitoh
K
K
Kashima
M
M
Shiozawa
A
A
Kozuka
M
M
Hashizume
H
H
Kimura
K
K
Yamazaki
M
M
Fujii
A
A
D000082:Acetaminophen / Q000494:pharmacology*; D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003300:Copper / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical / Q000378:metabolism*; D066298:In Vitro Techniques; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D012980:Sodium Salicylate / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D017665:Hydroxyl Radical; D000082:Acetaminophen; D003300:Copper; D006861:Hydrogen Peroxide; D012980:Sodium Salicylate