Dynamics of carriage of Neisseria meningitidis in a group of military recruits: subtype stability and specificity of the immune response following colonization.
Meningococcal carriage and the immune response to colonization were studied in a group of military recruits undergoing basic training. Subtyping by determination of the class 1 protein sequence clearly differentiated between strains and demonstrated the dynamics of carriage and transmission. Expression of class 1 protein by each strain remained stable during prolonged carriage by different subjects. Following colonization, a marked increase in serum bactericidal response occurred, which was specific for the subtype of the acquired strain and was associated with an increase in reactivity by Western blot to the homologous class 1 protein. Subjects colonized by multiple strains showed evidence of a specific immune response to the class 1 protein of each strain acquired. The subtype specificity of the bactericidal response to meningococci and the stability of expression of the class 1 protein have important implications for the design of vaccines for prevention of serogroup B meningococcal disease.
Molecular Microbiology Group, University of Southampton Medical School, Southampton General Hospital, United Kingdom. jeh@soton.ac.uk
G R
GR
Jones
M
M
Christodoulides
J L
JL
Brooks
A R
AR
Miller
K A
KA
Cartwright
J E
JE
Heckels
D000907:Antibodies, Bacterial / Q000097:blood; D000917:Antibody Formation; D001426:Bacterial Proteins / Q000276:immunology; D001770:Blood Bactericidal Activity; D006801:Humans; D008589:Meningococcal Infections / Q000097:blood / Q000276:immunology / Q000382:microbiology*; D008889:Military Personnel; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins