Inhibition of neuronal dopamine uptake by some antiallergic drugs.
The effects of 10 antiallergic drugs (astemizole, azelastine, ebastine, emedastine, epinastine, ketotifen, oxatomide, terfenadine, pemirolast and tranilast) on neuronal dopamine uptake were examined. Some drugs examined showed a concentration-dependent inhibition of [3H]dopamine uptake into synaptosomal preparations of the rat striatum. The inhibition constant (Ki) values were 231-876 nM for ebastine, terfenadine, oxatomide and astemizole. The specific binding of [3H] (1-[2-(diphenylmethoxy)ethyl]-4-(3-phenylpropyl)piperazine) (GBR12935) to the rat striatal membranes was also inhibited by these antiallergic drugs. There was a good correlation between the degrees of inhibition of [3H]dopamine uptake and [3H]GBR12935 binding. Then, the behavioral excitement induced by L-DOPA (100 mg/kg, s.c.) plus pargyline hydrochloride (80 mg/kg, i.p.) in mice was significantly enhanced by i.p. treatment with ebastine (10 mg/kg) and astemizole (5 mg/kg). These results suggest that the neuronal dopamine uptake is inhibited by some antiallergic drugs, especially ebastine.
Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
K
K
Matsunaga
T
T
Sato
H
H
Shuto
Y
Y
Tsuruta
K
K
Suemaru
Y
Y
Gomita
R
R
Oishi
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism*; D015259:Dopamine Agents / Q000494:pharmacology; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology*; D004357:Drug Synergism; D006634:Histamine H1 Antagonists / Q000494:pharmacology; D007980:Levodopa / Q000494:pharmacology; D008297:Male; D051379:Mice; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D010293:Pargyline / Q000494:pharmacology; D010879:Piperazines / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013268:Stimulation, Chemical; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism
D018926:Anti-Allergic Agents; D015259:Dopamine Agents; D018765:Dopamine Uptake Inhibitors; D006634:Histamine H1 Antagonists; D008996:Monoamine Oxidase Inhibitors; D010879:Piperazines; D007980:Levodopa; C044630:1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine; D010293:Pargyline; D004298:Dopamine