Calcium mobilization during nicotine-induced cell death in human glioma and glioblastoma cell lines.
Nicotine dose-dependently induced cytotoxicity in human glioma (KG-1-C) and glioblastoma (GBS-1, T98G) cell lines, but could not induce internucleosomal DNA cleavage, in contrast to apoptosing human myelogenous leukemic cell lines. Human glioma/glioblastoma cell lines thus might have a chromatin structure resistant to endonuclease digestion. Nicotine induced a rapid increase in the intracellular calcium concentration. Confocal experiments with Fluo-3 fluorescence revealed that nicotine elevated the free Ca2+ concentration in both nuclear and cytoplasmic regions of the cells, and the elevation of Ca2+ in the nuclear region was more pronounced than that of the cytoplasmic region. The present study suggests that nuclear accumulation of Ca2+ is an important initial step for cell death induction by nicotine.
Yamamura
M
M
Department of Biochemistry, School of Medicine, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Amano
Y
Y
Sakagami
H
H
Yamanaka
Y
Y
Nishimoto
Y
Y
Yoshida
H
H
Yamaguchi
M
M
Ohata
H
H
Momose
K
K
Takeda
M
M
D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D001932:Brain Neoplasms; D002118:Calcium / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects*; D053938:DNA Fragmentation; D004305:Dose-Response Relationship, Drug; D005909:Glioblastoma; D005910:Glioma; D006801:Humans; D007700:Kinetics; D009538:Nicotine / Q000633:toxicity*; D013997:Time Factors; D014407:Tumor Cells, Cultured
D009538:Nicotine; D002118:Calcium