The influence of advanced glycation endproducts (AGE) on the expression of human endothelial adhesion molecules.
Advanced glycation endproducts (AGEs) possibly play a dominant role in the pathogenesis of macrovascular disease in diabetes. Recent studies could demonstrate that glycated albumin (AGE-BSA) was able to stimulate vascular cell adhesion molecule-1 (VCAM.1) on endothelial cells. The aim of this study was to find out if AGE-BSA was not only able to enhance the expression of vascular cell adhesion molecule-1, but also of intercellular adhesion molecule-1 (ICAM-1) and E-Selectin on human endothelial cells. Stimulation of endothelial cells with AGE-BSA for six hours predominantly increased the expression of VCAM-1, but ICAM-1 and E-Selectin were also upregulated as shown by immunoilluminometric assay (ILMA).
Department of Internal Medicine and Endocrinology, University of Mainz, Germany. endokrin@mail.uni-mainz.de
T
T
Kunt
T
T
Forst
O
O
Harzer
G
G
Buchert
A
A
Pfützner
M
M
Löbig
A
A
Zschäbitz
E
E
Stofft
M
M
Engelbach
J
J
Beyer
D000418:Albumins / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D019040:E-Selectin / Q000187:drug effects / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D017127:Glycation End Products, Advanced / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D018799:Intercellular Adhesion Molecule-1 / Q000187:drug effects / Q000235:genetics; D008163:Luminescent Measurements; D016133:Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects / Q000235:genetics; D019010:Vascular Cell Adhesion Molecule-1 / Q000187:drug effects* / Q000235:genetics
D000418:Albumins; D019040:E-Selectin; D017127:Glycation End Products, Advanced; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1