A confocal microscopic evaluation of the effects of insulin imprinting on the binding of Concanavalin A by Tetrahymena Pyriformis.
With confocal microscopy it is possible to study the Concanavalin A (Con A) binding characteristics of the surface and interior of a single cell by viewing optical sections. It was observed in Tetrahymena pyriformis that Con A bound both to the plasma membrane and to intracellular structures. Incubation of cells with a competing sugar a-methylmannopyranoside, decreased binding. Hormonal imprinting with insulin resulted in an increase in binding of Con A to the cell surface and a decrease in intracellular binding. It is possible that the intracellular binding sites may migrate to the plasma membrane.
Department of Biology, Semelweis University of Medicine, Budapest, Hungary.
P
P
Kovács
C
C
Sundermann
B H
BH
Estridge
G
G
Csaba
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism / Q000494:pharmacology*; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D008766:Methylmannosides / Q000494:pharmacology; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011485:Protein Binding / Q000187:drug effects; D012235:Rhodamines; D013769:Tetrahymena pyriformis / Q000378:metabolism*
D005456:Fluorescent Dyes; D007004:Hypoglycemic Agents; D007328:Insulin; D008766:Methylmannosides; D012235:Rhodamines; D003208:Concanavalin A; C009434:tetramethylrhodamine isothiocyanate