Combined multiple sequence reduced protein model approach to predict the tertiary structure of small proteins.
By incorporating predicted secondary and tertiary restraints into ab initio folding simulations, low resolution tertiary structures of a test set of 20 nonhomologous proteins have been predicted. These proteins, which represent all secondary structural classes, contain from 37 to 100 residues. Secondary structural restraints are provided by the PHD secondary structure prediction algorithm that incorporates multiple sequence information. Predicted tertiary restraints are obtained from multiple sequence alignments via a two-step process: First, "seed" side chain contacts are identified from a correlated mutation analysis, and then, the seed contacts are "expanded" by an inverse folding algorithm. These predicted restraints are then incorporated into a lattice based, reduced protein model. Depending upon fold complexity, the resulting nativelike topologies exhibit a coordinate root-mean-square deviation, cRMSD, from native between 3.1 and 6.7 A. Overall, this study suggests that the use of restraints derived from multiple sequence alignments combined with a fold assembly algorithm is a promising approach to the prediction of the global topology of small proteins.
Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA.
A R
AR
Ortiz
A
A
Kolinski
J
J
Skolnick
D000465:Algorithms; D000595:Amino Acid Sequence; D003196:Computer Graphics; D003198:Computer Simulation; D008958:Models, Molecular; D009010:Monte Carlo Method; D011237:Predictive Value of Tests; D011381:Programming Languages; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry*; D016415:Sequence Alignment; D012984:Software
D011506:Proteins