Lipid metabolism in primary cultured hepatocytes from daunomycin-nephrotic rats.
We investigated the lipid metabolism in primary cultured hepatocytes to elucidate the causes of hyperlipidemia, increased cholesteryl esters, and decreased triglyceride levels in the livers of daunomycin-nephrotic rats. The incorporation of 14C-palmitate into phospholipids and triglycerides in primary cultured hepatocytes and medium was similar in daunomycin-nephrotic and control rats. The incorporation of 14C-acetate into phospholipids, triglycerides, cholesterol, cholesteryl esters, and total fatty acids in primary cultured hepatocytes was increased in daunomycin-nephrotic rats. The radioactivity of phospholipids, triglycerides, cholesterol, cholesteryl esters, and very-low-density lipoprotein lipids in medium was increased in the hepatocytes of daunomycin-nephrotic rats using 14C-acetate as a precursor. The increased cholesterogenesis and the increased secretion of triglycerides synthesized from acetate by hepatocytes may be due to an increased cholesteryl ester content and a decreased triglyceride content in the livers of daunomycin-nephrotic rats. The increased secretion of lipids synthesized from acetate by hepatocytes may be due to increased accumulation of lipids in serum and very-low-density lipoprotein in daunomycin-nephrotic rats.
Renal and Dialysis Units, Hyogo College of Medicine, Nishinomiya, Japan.
S
S
Gomikawa
Y
Y
Takamitu
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D003630:Daunorubicin; D005227:Fatty Acids / Q000096:biosynthesis; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009404:Nephrotic Syndrome / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D010168:Palmitates / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000096:biosynthesis
D000085:Acetates; D005227:Fatty Acids; D008055:Lipids; D010168:Palmitates; D014280:Triglycerides; D003630:Daunorubicin