Application of two-dimensional protein gels in biotechnology.
The optimal use of biological systems for technologically developed products will not be achieved until biological systems are completely defined in biochemical terms. Two-dimensional polyacrylamide gel electrophoresis, 2-D gels, are contributing to this goal. These gels separate complex mixtures of proteins into individual polypeptide species. The ultimate use of 2-D gels is the construction of cellular 2-D gel databases which identify the proteins on the gels and catalog their responses to different environmental conditions. In addition to these global analyses, many applications for 2-D gels in basic, applied and clinical research have been shown.
VanBogelen
R A
RA
Department of Biotechnology, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert, Ann Arbor, Michigan 48105, USA.
Olson
E R
ER
D000595:Amino Acid Sequence; D000818:Animals; D001709:Biotechnology / Q000379:methods; D019992:Databases as Topic; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D009154:Mutation; D011506:Proteins / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*
D011506:Proteins