Use of 5-nitroindole-2'-deoxyribose-5'-triphosphate for labelling and detection of oligonucleotides.
The 5'-triphosphate of 5-nitroindole-2'-deoxyriboside has been shown to be a good substrate for terminal deoxynucleotidyl transferase (TdT). An antibody has been prepared for the detection of 5-nitroindole and has been used for the detection of 5-nitroindole tailed DNA both in single-stranded form and after hybridisation to a template. This is therefore a new method for the detection of nucleic acid probes.
Smith
C L
CL
Amersham International plc, Amersham Laboratories, Buckinghamshire, UK.
Simmonds
A C
AC
Hamilton
A L
AL
Martin
D L
DL
Lashford
A G
AG
Loakes
D
D
Hill
F
F
Brown
D M
DM
D000906:Antibodies / Q000378:metabolism; D004253:DNA Nucleotidylexotransferase / Q000378:metabolism; D015342:DNA Probes / Q000737:chemistry; D003838:Deoxyadenine Nucleotides; D007211:Indoles / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis*
D000906:Antibodies; D015342:DNA Probes; D003838:Deoxyadenine Nucleotides; D007211:Indoles; D009841:Oligonucleotides; D004253:DNA Nucleotidylexotransferase; C026600:2'-deoxyadenosine triphosphate; C090067:5-nitroindole