Involvement of CD4+ T cells in the development of dextran sulfate sodium-induced experimental colitis and suppressive effect of IgG on their action.
1. To clarify the role of T cells in the development of dextran sulfate sodium (DSS)-induced colitis, T cells from colitis mice were primed with DSS-pulsed macrophages in vitro and then transferred into normal mice. In addition, to determine whether the target cell of immunoglobulin G (IgG) is the T cell, the extent of T cell proliferation induced by pulsed macrophages was examined after preincubation with IgG. 2. When mice receiving the primed T cells were treated with oral DSS, colitis symptoms were more severe than in animals treated with oral DSS only. This activity of primed T cells was reduced by depletion from the cells of CD4+ but not CD8+ cells. 3. The proliferation of T cells from colitis mice induced by pulsed macrophages was inhibited by T cell preincubation with homologous IgG. 4. The results suggest that CD4+ T cells play an important role in the development of DSS-induced experimental colitis and that IgG may modulate the development of colitis through interaction with pathogenic T cells.
Shintani
N
N
Research Division, The Green Cross Corporation, Hirakata, Osaka, Japan. ac46086@yoshitomi.co.jp
Nakajima
T
T
Okamoto
T
T
Kondo
T
T
Nakamura
N
N
Mayumi
T
T
D019264:Adoptive Transfer; D000818:Animals; D000961:Antilymphocyte Serum; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D016264:Dextran Sulfate / Q000494:pharmacology*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D016176:T-Lymphocyte Subsets / Q000276:immunology
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D016264:Dextran Sulfate