Separation from related compounds and assay of calcipotriol by high-performance liquid chromatography.
In this paper, two methods are presented. One involves the separation of calcipotriol, a new synthetic analogue, from two related compounds, specifically cholecalciferol (Vitamin D3) and calcitriol (1,25-dihydroxyvitamin D3). The other involves the isolation and assay of calcipotriol from a topical ointment. The study was performed with reversed-phase high-performance liquid chromatography using an RP18 column and ultraviolet detection. Applying the method of Snyder, a mobile phase mixture containing methanol-acetonitrile-water (67:23:10, v/v) was found which achieved a total separation within 18 min. A mobile phase of methanol-water (80:20, v/v) attained a slower elution of calcipotriol. For isolation and assay of calcipotriol from an ointment (Daivonex), dissolution in chloroform gave the highest recovery (> 98%). The isolation and assay process can be performed within 2 h.
Cirunay
J J
JJ
Department of Pharmaceutical Technology and Physical Pharmacy, Vrije Universiteit Brussel, Belgium.
Heyden
Y V
YV
Plaizier-Vercammen
J
J
D000097:Acetonitriles; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D002725:Chloroform; D002762:Cholecalciferol / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000432:Methanol; D011786:Quality Control; D013678:Technology, Pharmaceutical; D013997:Time Factors; D014867:Water
D000097:Acetonitriles; D014867:Water; C055085:calcipotriene; D002762:Cholecalciferol; D002725:Chloroform; D002117:Calcitriol; D000432:Methanol; C032159:acetonitrile