Effects of 24R,25-dihydroxyvitamin D3 on alkaline phosphatase activity in pig renal epithelial LLC-PK1 cells in culture.
1. The effects of 24R,25-dihydroxyvitamin D3 [24,25(OH)2D3] on alkaline phosphatase activity (ALP) were evaluated in pig kidney LLC-PK1 cells in culture. 2. The vitamin D3 metabolite increased ALP activity in these cells, whereas no effect of the hormone was observed on gamma-glutamyltranspeptidase and acid phosphatase activities. 3. ALP activity was stimulated after 3- to 12-hr incubation in the presence of 10(-9) mol/l 24,25(OH)2D3 with a maximum after 6 hr. 4. The hormonal induction of ALP activity was prevented by pretreatment of cells by actinomycin D. 5. It is proposed that 24,25(OH)2D3 could increase ALP activity by de novo protein synthesis.
Laboratoire de Pharmacologie/CHU-UFR de Pharmacie, Université d'Angers, France.
X D
XD
Thanh
F
F
Massicot
B
B
Do
R
R
Breget
D
D
Durand
J M
JM
Warnet
J R
JR
Claude
N
N
Taniguchi
J P
JP
Clot
D015650:24,25-Dihydroxyvitamin D 3 / Q000494:pharmacology*; D000135:Acid Phosphatase / Q000096:biosynthesis / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000096:biosynthesis / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004789:Enzyme Activation; D004790:Enzyme Induction; D004847:Epithelial Cells / Q000201:enzymology; D006728:Hormones / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects* / Q000201:enzymology; D018374:LLC-PK1 Cells; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D013552:Swine; D005723:gamma-Glutamyltransferase / Q000096:biosynthesis / Q000378:metabolism
D006728:Hormones; D015650:24,25-Dihydroxyvitamin D 3; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase