Electrophoretic profile of hybrids between cryotolerant and non-cryotolerant Saccharomyces strains.
The chromosomal DNAs of cryotolerant Saccharomyces bayanus, non-cryotolerant Saccharomyces cerevisiae strains and their intra and interspecific hybrids were separated by pulsed field electrophoresis (PFGE). The cryotolerant and non-cryotolerant strains gave distinctly different electrophoretic profiles. The hybrids cryotolerant x cryotolerant and non-cryotolerant x non-cryotolerant were fertile and they gave the same electrophoretic karyotype as the respective parents. The cryotolerant x non-cryotolerant hybrids were sterile and gave electrophoretic karyotypes which showed both the bands the parents have in common and those they do not share.
Istituto di Industrie Agrarie, University of Catania, Reggio Emilia, Italy.
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P
Giudici
C
C
Caggia
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A
Pulvirenti
C
C
Zambonelli
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S
Rainieri
D003080:Cold Temperature; D003433:Crosses, Genetic; D016172:DNA Fingerprinting; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification*; D016521:Electrophoresis, Gel, Pulsed-Field; D006824:Hybridization, Genetic; D007621:Karyotyping; D012440:Saccharomyces / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development; D013045:Species Specificity
D004271:DNA, Fungal