Immunoblotting of multiple antigenic peptides.
Multiple antigenic peptides (MAPs) can be efficiently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunoblot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS-PAGE as well as by Fast Green on an immunoblot. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS-PAGE and immunoblotting.
Kurien
B T
BT
Department of Arthritis and Immunology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA. kurienb@cpu2.omrf.ouhsc.edu
Jackson
K
K
Scofield
R H
RH
D000595:Amino Acid Sequence; D001324:Autoantigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D008969:Molecular Sequence Data
D001324:Autoantigens