The DNA binding affinity of rat liver nucleoproteins to the regulatory elements of the haptoglobin and alpha 2-macroglobulin genes.
Transcriptional regulation and binding interactions between soluble nucleoproteins and the hormone response elements (REs) of the rat haptoglobin (Hp) and alpha 2-macroglobulin (alpha 2MG) genes was examined in the livers of rats during the acute-phase reaction. Our results demonstrate that the elevation of the Hp and alpha 2MG genes' transcription rates in acute-phase liver relies essentially on an increase in the binding-affinity of pre-existing soluble nucleoproteins, enhancing their capability to bind the examined cis-regulatory elements. The 35kD nucleoprotein that displayed an acute-phase inducible affinity to bind hormone REs of rat Hp and alpha 2MG genes, was identified as a C/EBP beta isoform.
Molecular Biology Laboratory, Institute for Biological Research, Belgrade, Yugoslav Federal Republic.
M
M
Petrović
D
D
Bogojević
I
I
Grigorov
L
L
Sevaljević
D000209:Acute-Phase Proteins / Q000032:analysis / Q000235:genetics; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins / Q000032:analysis / Q000378:metabolism*; D006242:Haptoglobins / Q000235:genetics*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D015533:Transcriptional Activation / Q000502:physiology; D000511:alpha-Macroglobulins / Q000235:genetics*
D000209:Acute-Phase Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D006242:Haptoglobins; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000511:alpha-Macroglobulins