Influence of biliary obstruction on neutrophil chemotaxis.
The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-beta), in rats that received 7-day bile duct ligation. Carrageenin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later for measurement of myeloperoxidase activity. In vitro chemotaxis of peripheral neutrophils to IL-8 was evaluated by a modified Boyden chamber method. Both carrageenin and IL-8 induced significantly pronounced intra-air pouch neutrophil recruitment in the bile duct-ligated group compared with a sham-ligated group. In vitro neutrophil chemotaxis was significantly increased in the bile duct-ligated group compared with the sham-ligated group. The present experimental model suggests enhanced neutrophil chemotaxis to IL-8 in obstructive jaundice.
Third Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan.
K
K
Yanagitani
Y
Y
Kubota
K
K
Tsuji
S
S
Yamamoto
Y
Y
Amoh
M
M
Takaoka
M
M
Ogura
K
K
Inoue
D000818:Animals; D002351:Carrageenan / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D002779:Cholestasis / Q000201:enzymology / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D016209:Interleukin-8; D002351:Carrageenan; D009195:Peroxidase