Chitosan-chondroitin sulfate and chitosan-hyaluronate polyelectrolyte complexes: biological properties.
In this work, we compare some biological properties of a highly deacetylated chitosan to those obtained with the materials made from its polyelectrolyte complexes with various GAG's such as chondroitin-sulfates and hyaluronic acid. The hydrolysis of the complexes by means of the specific hydrolytic enzymes is studied. Cell-adhesion and cell-proliferation on these materials is compared to that obtained with a pure chitosan material. Finally, a series of in vivo experiments is performed to test the wound-healing properties of this kind of complexes. All the results agree to show that chitosan has a protective effect against GAG's hydrolysis by their specific enzymes but only at pH's different from the optimal pH of the enzyme considered. In addition, they also agree to confirm that a pure chitosan material gives the best results in connection with cell-attachment and cell-proliferation or wound healing. Nevertheless, whatever the case, no adverse effect was observed with the polyelectrolyte complexes GAG's-chitosan.
Laboratoire d'Etude des Matériaux Plastiques et des Biomatériaux (UMR CNRS 5627), Université Claude Bernard, Villeurbanne, France.
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Denuziere
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Ferrier
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Damour
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Domard
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001704:Biopolymers; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002686:Chitin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D048271:Chitosan; D019902:Chondrocytes / Q000187:drug effects* / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000494:pharmacology*; D004573:Electrolytes; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects
D001672:Biocompatible Materials; D001704:Biopolymers; D004573:Electrolytes; D002686:Chitin; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D048271:Chitosan