Difference in susceptibility to CC-chemokines among HIV-1 isolates.
In this study, we examined the difference in susceptibility to anti-HIV activity of the CC-chemokines (RANTES, MIP-1 alpha and MIP-1 beta) among HIV-1 isolates and analysed its relation with phenotype (syncytium inducibility) and V3 domain of gp120 of the HIV-1 isolates. Of 11 cases tested in endogenous assay, at a concentration of 200 ng/ml, RANTES, MIP-1 alpha, and MIP-1 beta showed more than 80% suppression of HIV-1 replication in 10, 8, and 7 cases, respectively. HIV-1 isolates sensitive to more than one CC-chemokine showed non-syncytium-inducing phenotype, whereas HIV-1 isolates resistant to all of the 3 CC-chemokines showed syncytium-inducing phenotype. HIV-1 isolates resistant to all of the 3 CC-chemokines contained more positively charged amino acid residues in the V3 domain of the gp120. These results indicated that utilization of the CC-chemokine receptors as co-receptors for virus entry could vary among HIV-1 isolates.
Research Institute for Microbial Diseases, Osaka University, Japan.
M
M
Gao
H
H
Tsuchie
T Q
TQ
Jin
J
J
Zhang
M A
MA
Detorio
M M
MM
Hossain
K
K
Taniguchi
T
T
Kurimura
D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000494:pharmacology; D018925:Chemokines / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins