Proteolytic ratchets that control progression through mitosis.
A key player in mitotic progression is a cell-cycle-regulated ubiquitin-protein ligase complex known as the anaphase-promoting complex or cyclosome (APC/C). The APC/C is part of the machinery that promotes the metaphase-anaphase transition by mediating the ubiquitin-dependent destruction of anaphase inhibitors and initiates exit from mitosis by degrading mitotic cyclins. This review describes the known components and substrates of the mitotic ubiquitination machinery and discusses how a new subfamily of proteins that contain the WD40 repeat (the Fizzy/Cdc20p family) might activate the APC/C to allow temporal differences in substrate ubiquitination during progression through mitosis.
Townsley
F M
FM
Dept of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.  fionat@warren.med.harvard.edu
Ruderman
J V
JV
Dept of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.  ruderman@warren.med.harvard.edu
D064173:Anaphase-Promoting Complex-Cyclosome; D005656:Fungal Proteins / Q000502:physiology; D008025:Ligases / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases; D014452:Ubiquitins / Q000502:physiology*; D015003:Yeasts / Q000166:cytology / Q000201:enzymology*
D005656:Fungal Proteins; D014452:Ubiquitins; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D008025:Ligases