Modulation of porphyrin derivatives accumulation in C6 glioma cells by drugs acting on beta-adrenergic receptors. A spectrofluorometric study.
The pharmacological modulation of the uptake of porphyrin derivatives in cultured C6 glioma cells was investigated by means of spectrofluorometric analysis both in single cells and in cell homogenates. The influence of drugs acting as beta-receptor agonists or antagonists was studied in cells grown to semiconfluency. Isoproterenol (ISO), a beta-receptor agonist, enhanced the intracellular fluorescence intensity of both Photofrin and protoporphyrin IX (PpIX). A treatment with a beta-receptor antagonist I-propranolol (PRO), simultaneous with ISO, resulted in an intracellular Photofrin fluorescence signal comparable to that of the control cells, indicating the specificity of the pharmacological action. The pharmacological treatment seemed particularly effective with the aggregated species. This is suggested by the relative increase of the band at 670 nm, being greater than that in the 630 nm band in the emission spectra of Photofrin and PpIX, and by the comparison of the fluorescence intensity on cell homogenates measured both in the absence and in the presence of cetyltrimethyl-ammonium bromide as a detergent.
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Centro di Studio per I'Istochimica, Università, Pavia, Italy.
Mares
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Lisa
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Krajci
D
D
Bottiroli
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G
D000818:Animals; D017323:Dihematoporphyrin Ether / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D018462:Photobiology; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011524:Protoporphyrins / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
D017319:Photosensitizing Agents; D011166:Porphyrins; D011524:Protoporphyrins; D011943:Receptors, Adrenergic, beta; D017323:Dihematoporphyrin Ether; D011433:Propranolol; C028025:protoporphyrin IX; D007545:Isoproterenol