Gamma irradiation and ozone treatment for inactivation of Escherichia coli O157:H7 in culture media.
A study was conducted to investigate the reduction and elimination of Escherichia coli O157:H7 by the effects of gamma irradiation and ozone treatment. Log phase cells were found to be more sensitive to gamma irradiation than stationary phase cells. E. coli O157:H7 was found to be considerably more resistant to irradiation at -18 degrees C than at 20 degrees C. The D values for this organism for treatment with ozone in tryptic soy agar were higher than those for treatment with ozone in phosphate buffer. Gamma irradiation at a dose of 1.5 kGy or ozone treatment at a concentration of 3 to 18 ppm for 20 to 50 min was required to assure the elimination of E. coli O157:H7.
Department of Food Irradiation, Korea Atomic Energy Research Institute, Taejon, Korea. mwbyun@nanum.kaeri.re.kr
M W
MW
Byun
O J
OJ
Kwon
H S
HS
Yook
K S
KS
Kim
D002021:Buffers; D003470:Culture Media; D019453:Escherichia coli O157 / Q000187:drug effects* / Q000528:radiation effects*; D005720:Gamma Rays; D010083:Oxidants, Photochemical; D010126:Ozone; D013696:Temperature
D002021:Buffers; D003470:Culture Media; D010083:Oxidants, Photochemical; D010126:Ozone