Molecular cloning of the bile salt-dependent lipase of ferret lactating mammary gland: an overview of functional residues.
Ferret lactating mammary gland bile salt-dependent lipase (BSDL, EC 3.1.1.-) has been cloned by RT-PCR. The open reading frame consists of 1869 nucleotides which encode 623 amino acids of the functional enzyme. When compared to other species, the greatest homology is observed between residues 1 and 484, with little or no homology at the C-terminal end where seven repeated segments of similar sequence are located. Ferret mammary gland BSDL retains residues involved in the active site and the tentative heparin binding site at similar positions in comparison to other milk or pancreatic BSDL. Other important items, such as binding peptide to chaperone molecular, phosphorylation site(s) or bile salt binding sites, were also tentatively located in well conserved regions of seven available BSDL sequences.
Sbarra
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V
INSERM U260, Unité de Recherches Physiologie des Relations Hormono-Nutritionnelles, Faculté de Médecine-Timone, 27 bld Jean Moulin, 13385 Marseille Cedex 05, France.
Bruneau
N
N
Mas
E
E
Hamosh
M
M
Lombardo
D
D
Hamosh
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001647:Bile Acids and Salts / Q000032:analysis; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D005289:Ferrets; D006801:Humans; D007774:Lactation; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008894:Milk Proteins / Q000096:biosynthesis*; D008969:Molecular Sequence Data; D002787:Sterol Esterase / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics
D001647:Bile Acids and Salts; D018076:DNA, Complementary; D008894:Milk Proteins; C054784:bile salt-stimulated lipase; D002787:Sterol Esterase