Laser light scattering immunoassay: an improved data analysis by CONTIN method.
Laser light scattering immunoassay (LIA) is a diagnostic method for the detection of antibody by monitoring the agglutination of antigen carrier particles mediated by antibody, using dynamic light scattering (DLS) as probe. We have used this method for the detection of antibody to P. falciparum that cause malaria. The data were analysed using CONTIN method and the superiority of the distribution analysis over the conventional interpretation of the data in terms of mean diffusion coefficient or hydrodynamic radius is discussed in detail.
School of Physical Sciences, Centre for Biotechnology, Jawaharlal Nehru University, New Delhi, India.
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Antony
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Saxena
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Roy
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Bohidar
D000371:Agglutination / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions / Q000276:immunology; D004058:Diffusion; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D008863:Microspheres; D010316:Particle Size; D010963:Plasmodium falciparum / Q000276:immunology; D012542:Scattering, Radiation