Metabolism of prostaglandins in porcine liver transplantation with a graft harvested after 30- and 60-minute warm ischemia.
The influence of warm ischemia on the metabolism of prostaglandins was investigated using a pig liver transplantation model employing the temporary portal arterialization technique. Eighteen pigs were divided into three groups according to warm ischemia time: 0 min (group I, n = 6), 30 min (group II, n = 6), and 60 min (group III, n = 6). During portal arterialization, the hepatic venous prostaglandin E2 (PGE2) level in group III (3356.0 +/- 1011.8 pg/ml) was significantly higher than that in group I (831.7 +/- 182.1 pg/ml; P = 0.0285). The hepatic venous PGE2 levels were significantly higher than the arterial counterparts in all groups both at the beginning and during portal arterialization. At 60 min after portal revascularization, the arterial PGE2 level in group III (886.7 +/- 268.0 pg/ml) was significantly higher than that in group I (99.0 +/- 18.6 pg/ml; P = 0.0116) and II (204.2 +/- 65.4 pg/ml; P = 0.0282). Neither thromboxane B2 (TXB2) nor 6-keto PGF1 alpha showed any significant differences. In conclusion, the intraoperative changes of PGE2 thus reflected the degree of warm ischemic damage, and PGE2 could also be released from the graft. On the other hand, the increased levels of TXB2 and 6-keto PGF1 alpha were thought to have an extrahepatic origin.
Isai
H
H
First Department of Surgery, Hokkaido University School of Medicine, Sapporo, Japan.
Kimura
J
J
Ito
K
K
Tamura
M
M
Kon
H
H
Kamachi
H
H
Takahashi
M
M
Yamashita
K
K
Shimamura
T
T
Nakajima
Y
Y
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D006499:Hepatic Artery; D006503:Hepatic Veins; D007511:Ischemia; D008099:Liver / Q000098:blood supply*; D016031:Liver Transplantation / Q000379:methods* / Q000502:physiology; D011453:Prostaglandins / Q000378:metabolism*; D013552:Swine; D013696:Temperature
D015415:Biomarkers; D011453:Prostaglandins