GB virus C/hepatitis G virus isolates in Japanese haemophiliacs and their origins.
Japanese haemophiliacs have been at high risk for infection with parenterally-transmissible viruses through the use of blood products, especially imported ones. Recently, novel transfusion-transmissible virus, GB virus C (GBV-C)/hepatitis G virus (HGV) were isolated. We investigated the origin and route of transmission of GBV-C/HGV isolates in haemophiliacs in Japan. GBV-C/HGV RNA was measured by nested reverse transcription polymerase chain reaction in 91 Japanese haemophiliacs. Phylogenetic analysis and genotypic grouping of GBV-C/HGV isolates in Japanese haemophiliacs were performed based on sequences in the 5' untranslated region, and the characteristics were compared with those of reported isolates. GBV-C/HGV infection was present in 19 of 91 haemophiliacs (20.9%). Sequence analysis showed that 15 of the 19 isolates (78.9%) showed sequence similarity to a group in which mainly West African isolates have been reported. The other 4 isolates (21.1%) showed sequence similarity to Asian isolates. None of the GBV-C/HGV isolates showed sequences similar to those generally found in isolates from USA and Europe. The majority of GBV-C/HGV isolates found in Japanese haemophiliacs who are considered to have been infected by imported blood products were similar to those detected in West Africa.
Second Department of Internal Medicine, Nagoya University School of Medicine, Japan.
H
H
Toyoda
Y
Y
Fukuda
T
T
Hayakawa
J
J
Takamatsu
H
H
Saito
H
H
Okamoto
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D018067:Flaviviridae / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006467:Hemophilia A / Q000821:virology*; D006525:Hepatitis, Viral, Human / Q000453:epidemiology*; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence