Protective effects of nicergoline against hydrogen peroxide toxicity in rat neuronal cell line.
We examined the effects of nicergoline on hydrogen peroxide (H2O2)-induced neurotoxicity in cultured rat neuronal cell line (B50). H2O2 induced death of B50 cells in a dose-dependent manner. The H2O2-induced neuronal cell death was significantly decreased in B50 cells maintained in the presence of nicergoline. We compared the levels of antioxidants (glutathione, catalase and superoxide dismutase) in nicergoline-treated and untreated B50 cells. Lipid peroxidation products (thiobarbituric acid reactive substances, TBARS) levels were also measured. Cultures treated with nicergoline had higher levels of catalase activity. TBARS level was significantly lower in nicergoline-treated cells than in untreated cells. Our results suggest that nicergoline may induce the up-regulation of intracellular antioxidant defences and protect the neuronal cells against oxidative stress.
Department of Neuroscience, Institute of Molecular and Cellular Medicine, Okayama University Medical School, Japan.
E
E
Iwata
I
I
Miyazaki
M
M
Asanuma
A
A
Iida
N
N
Ogawa
D000818:Animals; D002374:Catalase / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000633:toxicity*; D015227:Lipid Peroxidation / Q000187:drug effects; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009530:Nicergoline / Q000494:pharmacology*; D018697:Nootropic Agents / Q000494:pharmacology; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
D018697:Nootropic Agents; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D009530:Nicergoline