Effect of arbutin on melanogenic proteins in human melanocytes.
The inhibitory effect of arbutin, a naturally occurring beta-D-glucopyranoside derivative of hydroquinone, on melanogenesis was studied biochemically by using human melanocytes in culture. Cells were cultured in the presence of different concentrations of arbutin. The maximum concentration of arbutin that was not inhibitory to growth of the cells was 100 micrograms/ml. At that concentration, melanin synthesis was inhibited significantly by approximately 20% after 5 days, compared with untreated cells. This phenotypic change was associated with the inhibition of tyrosinase and DHICA polymerase activities, and the degree of inhibition was dose dependent. No significant difference in DOPAchrome tautomerase (DT) activity was observed before or after arbutin treatment. Western blotting experiments revealed there were no changes in protein content or in molecular size of tyrosinase, TRP-1 or TRP-2, indicating that inhibition of tyrosinase activity by arbutin might be due to effects at the post-translational level.
Department of Dermatology, Kobe University School of Medicine, Japan.
A K
AK
Chakraborty
Y
Y
Funasaka
M
M
Komoto
M
M
Ichihashi
D000293:Adolescent; D000328:Adult; D001104:Arbutin / Q000494:pharmacology*; D015153:Blotting, Western; D002452:Cell Count; D002478:Cells, Cultured; D006801:Humans; D007211:Indoles / Q000378:metabolism; D019746:Intramolecular Oxidoreductases / Q000276:immunology / Q000378:metabolism; D008297:Male; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects* / Q000201:enzymology; D008562:Membrane Glycoproteins; D014442:Monophenol Monooxygenase / Q000276:immunology / Q000378:metabolism; D010088:Oxidoreductases; D011108:Polymers / Q000378:metabolism; D011506:Proteins / Q000276:immunology
D007211:Indoles; D008543:Melanins; D008562:Membrane Glycoproteins; D011108:Polymers; D011506:Proteins; C030692:5,6-dihydroxy-2-indolylcarboxylic acid; D001104:Arbutin; D010088:Oxidoreductases; C489799:TYRP1 protein, human; C064056:tyrosinase-related protein-1; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase