A fast and inexpensive method for isolation of high-quality RNA from eimerian oocysts.
An inexpensive and simple method using acid-phenol was developed for the isolation of total RNA from eimerian oocysts. This procedure provides a pure preparation of undegraded RNA in high yield and can be completed in under 2 h.
Johnston
D A
DA
Department of Pathobiology, University of Guelph, Ontario, Canada.
Bratty
J
J
Fernando
M A
MA
D000818:Animals; D002645:Chickens; D003365:Costs and Cost Analysis; D004539:Eimeria / Q000235:genetics*; D006146:Guanidines; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010274:Parasitology / Q000379:methods*; D019800:Phenol; D016053:RNA, Protozoan / Q000302:isolation & purification*; D013861:Thiocyanates; D013997:Time Factors; D014269:Trifluoroacetic Acid
D006146:Guanidines; D007202:Indicators and Reagents; D016053:RNA, Protozoan; D013861:Thiocyanates; D019800:Phenol; C054436:guanidine thiocyanate; D014269:Trifluoroacetic Acid