Identification of two novel palindromic regulatory elements in the murine interleukin-5 promoter.
Interleukin-5 has remarkable specificity for the eosinophil lineage. This fact, combined with the biological specificity of eosinophilia suggests tight and independent regulation of IL-5 expression. Here we report two novel palindromic regulatory elements (PRE) which contain positive regulatory motifs (PRM) that control transcription of the murine IL-5 gene. The first element, mPRE1-IL5 (-79 to -90) contains the mPRM1 at positions -87 to -89 which operates as a positive regulatory element with mutation of this motif resulting in a 64% decrease in gene activity. Gene expression was reduced by 67% when a similar mutation was introduced into the mPRM2 (-467 to -469) of mPRE2-IL5 (-459 to -470). Both elements specifically bind proteins from EL4-23 cell nuclear extracts forming constitutive DNA-protein complexes. EMSA experiments utilising mutated mPRE-IL5 oligonucleotides indicate that in both elements, the mPRMs are essential for protein binding.
Division of Molecular Immunology, TVW Telethon Institute for Child Health Research and the University of Western Australia, West Perth, Australia.
G T
GT
Schwenger
V A
VA
Mordvinov
S
S
Karlen
M
M
D'Ercole
C J
CJ
Sanderson
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D005809:Genes, Regulator; D015848:Interleukin-5 / Q000235:genetics*; D008156:Luciferases / Q000235:genetics; D051379:Mice; D009154:Mutation; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D014162:Transfection
D017931:DNA Primers; D015848:Interleukin-5; D004247:DNA; D008156:Luciferases