Extra-weak chemiluminescence produced by autoxidation of kampo extract preparations stored in heat-stressed conditions.
The purpose of this study was to evaluate the usefulness of extra-weak chemiluminescence (CL) measurement as a rapid method to estimate the stability of Kampo extract preparations. It was found that the Kampo drugs that emit little CL were stable, while those with higher CL were comparatively unstable with regard to the various stability markers, including change of coloration (browning), contents of specific ingredients, high molecular compounds, amino acids and sugars under various conditions of heat storage. Excellent correlation existed between the CL of Kampo drugs and the coloration (delta E* (ab)) and the other above-mentioned evaluation markers. From this investigation, it was deduced that the CL of Kampo drugs originates in the early stage of the Maillard reaction and reflects the stability of the preparations, and that CL is useful for estimating the stability of Kampo drugs.
Department of Pharmaceutical Sciences, Tohoku University Hospital, Sendai, Japan.
H
H
Sato
H
H
Hirayama
T
T
Yamamoto
F
F
Ishizawa
M
M
Mizugaki
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D006358:Hot Temperature; D008163:Luminescent Measurements; D008516:Medicine, Chinese Traditional; D008970:Molecular Weight
D000596:Amino Acids; D002241:Carbohydrates; D004365:Drugs, Chinese Herbal