A missense mutation of the gene for Na+,K(+)-ATPase alpha-subunit causes abnormal feeding behavior in Caenorhabditis elegans.
The Na+,K(+)-ATPase activity of membranes from a behavioral mutant of C. elegans was found to be about one-third that of the wild-type. The levels of mRNA and polypeptide of Na+,K(+)-ATPase alpha-subunit in the mutant were as high as those in the wild-type, but the level of the phosphorylated intermediate of the Na+,K(+)-ATPase in the mutant worm was 80% lower than that in the wild-type. A single predicted amino acid replacement (Leu to Phe) was found in a highly conserved region in the alpha-subunit that is involved in the formation of phosphorylated intermediate. The abnormal feeding behavior of the mutant worm may be attributed to this missense mutation.
Shima
Y
Y
Laboratory of Biochemistry, Kyorin University School of Health Sciences, Tokyo, Japan.
Tada
Y
Y
Furuki
M
M
Hara
Y
Y
Ohta
H
H
D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D002462:Cell Membrane / Q000201:enzymology; D017124:Conserved Sequence; D018509:DNA, Helminth / Q000235:genetics; D005247:Feeding Behavior; D017238:Genes, Helminth; D007700:Kinetics; D046911:Macromolecular Substances; D008969:Molecular Sequence Data; D010042:Ouabain / Q000494:pharmacology; D010766:Phosphorylation; D017354:Point Mutation; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D000254:Sodium-Potassium-Exchanging ATPase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014158:Transcription, Genetic
D018509:DNA, Helminth; D046911:Macromolecular Substances; D012333:RNA, Messenger; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase