Rapid electrophoretic separation of pre-beta-migrating high density lipoproteins using automated PhastSystem: application to analysis of lecithin:cholesterol acyltransferase-deficient plasma.
To identify pre-beta-high density lipoproteins, a rapid two-dimensional separation by electrophoresis (1 hour 30 minutes) was performed on an automated Phast System. This procedure used commercially available polyacrylamide gradient gels (4-15%) and allows sensitive and reproducible results. Pre-beta-1- and pre-beta-2-high density lipoproteins were clearly identified by this method. In addition, our procedure was successfully applied to diagnosis of a patient with familial lecithin:cholesterol acyltransferase deficiency, characterized by the absence of alpha-high density lipoproteins.
Saïdi
Y
Y
Laboratoire des Lipides et Lipoprotéines, Hôpital de la Pitié, Paris, France.
Sich
D
D
Federspiel
M C
MC
Auer
C
C
Egloff
M
M
Castro
G R
GR
Beucler
I
I
D001331:Automation; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007863:Lecithin Cholesterol Acyltransferase Deficiency / Q000097:blood* / Q000175:diagnosis; D008075:Lipoproteins, HDL / Q000302:isolation & purification*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D011498:Protein Precursors / Q000302:isolation & purification*
D008075:Lipoproteins, HDL; D011498:Protein Precursors; D007862:Phosphatidylcholine-Sterol O-Acyltransferase