Search for genes involved in UV-resistance in human cells by mRNA differential display: increased transcriptional expression of nucleophosmin and T-plastin genes in association with the resistance.
UVr-10 UV-resistant cells were established from UV-sensitive human RSb cells. We searched here for genes expressed differentially between UVr-10 and RSb cells using a differential display method to elucidate the molecular mechanisms underlying the UV-resistance. Thirteen candidate cDNA fragments were obtained from 514 mRNA species first screened. Among the cDNA fragments, 3 revealed increased mRNA expression in UVr-10 cells compared with RSb cells by Northern analysis. Nucleotide sequence analysis identified 2 of the 3 cDNA fragments as encoding nucleophosmin (NPM) and T-plastin. The expression of NPM mRNA was induced after UV irradiation in UVr-10 cells but not in RSb cells, whereas irradiation did not affect the expression of T-plastin mRNA. UVr-10 cells transfected with antisense cDNA for NPM mRNA were partially sensitized to UV cell-killing. Thus, NPM and possibly T-plastin genes may contribute to the increased resistance to UV cell-killing, at least in the human cells tested.
Department of Neurosurgery, School of Medicine, Chiba University, Japan.
Y
Y
Higuchi
K
K
Kita
H
H
Nakanishi
X L
XL
Wang
S
S
Sugaya
H
H
Tanzawa
H
H
Yamamori
K
K
Sugita
A
A
Yamaura
N
N
Suzuki
D001483:Base Sequence; D002460:Cell Line; D002999:Clone Cells; D017931:DNA Primers; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics*; D000090243:Nucleophosmin; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000096:biosynthesis; D011836:Radiation Tolerance / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic / Q000528:radiation effects*; D014466:Ultraviolet Rays
D017931:DNA Primers; D008562:Membrane Glycoproteins; D008840:Microfilament Proteins; C000716967:NPM1 protein, human; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D012333:RNA, Messenger; C026742:plastin; D000090243:Nucleophosmin