KCR1, a membrane protein that facilitates functional expression of non-inactivating K+ currents associates with rat EAG voltage-dependent K+ channels.
Cerebellar granule neurons possess a non-inactivating K+ current, which controls resting membrane potentials and modulates the firing rate by means of muscarinic agonists. kcr1 was cloned from the cerebellar cDNA library by suppression cloning. KCR1 is a novel protein with 12 putative transmembrane domains and enhances the functional expression of the cerebellar non-inactivating K+ current in Xenopus oocytes. KCR1 also accelerates the activation of rat EAG K+ channels expressed in Xenopus oocytes or in COS-7 cells. Far-Western blotting revealed that KCR1 and EAG proteins interacted with each other by means of their C-terminal regions. These results suggest that KCR1 is the regulatory component of non-inactivating K+ channels.
Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine, Kanazawa 920-8640, Japan.
N
N
Hoshi
H
H
Takahashi
M
M
Shahidullah
S
S
Yokoyama
H
H
Higashida
D000595:Amino Acid Sequence; D000818:Animals; D002531:Cerebellum / Q000502:physiology*; D003001:Cloning, Molecular; D016373:DNA, Antisense / Q000494:pharmacology; D018076:DNA, Complementary / Q000235:genetics; D004553:Electric Conductivity; D051638:Ether-A-Go-Go Potassium Channels; D015640:Ion Channel Gating / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000502:physiology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014981:Xenopus
C114599:Alg10 protein, rat; D016373:DNA, Antisense; D018076:DNA, Complementary; D051638:Ether-A-Go-Go Potassium Channels; D009419:Nerve Tissue Proteins; D015221:Potassium Channels; D012333:RNA, Messenger; D011994:Recombinant Proteins; D008274:Magnesium; D011188:Potassium