Convenient approaches to the synthesis of oligonucleotide macrocycles containing non-nucleotide linkers.
Two convenient, practical routes to the synthesis of non-nucleotide bridged cyclic oligonucleotides have been developed. The first procedure included circularization of oligonucleotides by template-directed ligation on solid phase, while the second procedure involved preparation of a circular oligomer by non-template chemical ligation of a linear precursor in solution. Using these approaches, a series of single- and double-stranded cyclic oligonucleotides with non-nucleotide bridges has been synthesized.
Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. eva@ibch.siobc.ras.ru
V A
VA
Efimov
A A
AA
Buryakova
A L
AL
Kalinkina
M V
MV
Choob
O G
OG
Chakhmakhcheva
J O
JO
Ojwang
R F
RF
Rando
D019380:Anti-HIV Agents; D002460:Cell Line; D004270:DNA, Circular / Q000138:chemical synthesis*; D006801:Humans; D019429:Integrase Inhibitors / Q000138:chemical synthesis; D008968:Molecular Conformation; D015394:Molecular Structure; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011409:Propylene Glycols / Q000138:chemical synthesis; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection / Q000235:genetics
D019380:Anti-HIV Agents; D004270:DNA, Circular; D019429:Integrase Inhibitors; D009841:Oligonucleotides; D011409:Propylene Glycols