Selective hydrolysis of milk proteins to facilitate the elimination of the ABBOS epitope of bovine serum albumin and other immunoreactive epitopes.
Milk proteins are hydrolyzed to prevent immunological reactions, but immunoreactive epitopes, including the ABBOS epitope of bovine serum albumin (BSA), can still be detected in commercially available milk protein hydrolysates. We used lactococcal cell-envelope proteinase (CEP) for the hydrolysis of the individual milk proteins and of mixtures thereof, or for the hydrolysis of sodium caseinate (contaminated with whey proteins). CEP exclusively degraded casein, leaving the four major whey proteins intact. This property facilitated the removal of the intact whey proteins from the casein fragments by ultrafiltration. Depending on the molecular mass of the whey protein to be removed, membranes with cutoff values between 3 and 30 kDa were used, resulting in casein hydrolysates free of protein fragments with cross-reactive whey-protein-specific IgE (immunoglobulin E) or ABBOS antibody-binding sites. Even the casein itself was degraded in such a way by CEP that cross-reactive casein-specific IgE antibody-binding sites could be eliminated. The product could find application in infant formulas for therapeutic and preventive treatment of children with cow's milk allergy; in addition, the preventive use of such formulas in children genetically susceptible to the development of insulin-dependent diabetes mellitus (IDDM) should be considered if a relationship between the consumption of BSA and IDDM were to become more apparent. The method is also applicable for preparing casein-free whey protein preparations.
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede, The Netherlands. alting@nizo.nl
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Alting
R J
RJ
Meijer
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EC
van Beresteijn
D000818:Animals; D002417:Cattle; D003922:Diabetes Mellitus, Type 1 / Q000517:prevention & control*; D000939:Epitopes; D006801:Humans; D006868:Hydrolysis; D007073:Immunoglobulin E / Q000276:immunology; D017045:Lactococcus / Q000201:enzymology; D008894:Milk Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000939:Epitopes; D008894:Milk Proteins; D010446:Peptide Fragments; C075490:serum albumin, bovine (152-168); D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E