Ultrastructural apoptotic lesions induced in rat thymocytes after borax ingestion.
Apoptosis has gained increasing attention in recent years. Several chemical compounds induce apoptotic lesions in the thymus. Male Wistar rats received 2000 ppm of borax (Na2B4O7.10H2O) in their food for 16 days. The rats were sacrificed 2, 5, 9, 12, 19, 21, 26 and 28 days after the beginning of treatment. Thymus samples of all rats were taken. A Philips EM 300 electron microscopy was used to study the ultrastructural morphology. Serious nuclear and cytoplasmic lesions were observed. Moreover, numerous macrophages containing apoptotic cells were present in the thymus. The alterations were observed from the 2nd to the 28th day. The extent of damage was much more important in the rats sacrificed 21, 26 and 28 days after borax ingestion.
Laboratoire de Biophysique de la Faculté de Médecine, Créteil, France.
I C
IC
Sylvain
J P
JP
Berry
P
P
Galle
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001881:Borates / Q000008:administration & dosage / Q000633:toxicity*; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D003593:Cytoplasm / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology* / Q000648:ultrastructure
D001881:Borates; C018851:borax