Effect of prolonged staining with hematoxylin on detecting Helicobacter pylori in hematoxylin-eosin-stained gastric mucosa.
Helicobacter pylori (H. pylori) is identifiable in hematoxylin-eosin (HE)-stained preparations, particularly under a prolonged hematoxylin staining condition. The staining intensity of H. pylori bodies was increased when the hematoxylin staining period was doubled from 5 min, the routine period of Meyer's hematoxylin staining, to 10 min. The staining intensity of the background epithelial nuclei was only mildly increased. Hematoxylin-eosin staining employing a prolonged hematoxylin staining period gave detectability of H. pylori comparable to May-Giemsa and immunoperoxidase staining. The modified HE method is thus very useful and practical for identifying H. pylori in routine gastric biopsy specimens.
Department of Pathology, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
K
K
Tazawa
Y
Y
Tsutsumi
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001399:Azure Stains; D001706:Biopsy; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D005189:False Positive Reactions; D005260:Female; D005753:Gastric Mucosa / Q000382:microbiology* / Q000473:pathology; D005756:Gastritis / Q000175:diagnosis* / Q000382:microbiology; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000382:microbiology; D016480:Helicobacter pylori; D006416:Hematoxylin; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D013194:Staining and Labeling / Q000379:methods
D001399:Azure Stains; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin