Nucleolar localization of early tRNA processing.
There is little information as to the location of early tRNA biosynthesis. Using fluorescent in situ hybridization in the budding yeast, Saccharomyces cerevisiae, examples of nuclear pre-tRNAs are shown to reside primarily in the nucleoli. We also probed the RNA subunit of RNase P. The majority of the signal from RNase P probes was nucleolar, with less intense signals in the nucleoplasm. These results demonstrate that a major portion of the tRNA processing pathway is compartmentalized in nucleoli with rRNA synthesis and ribosomal assembly. The spatial juxtaposition suggests the possibility of direct coordination between tRNA and ribosome biosynthesis.
Bertrand
E
E
Institut de Genetique Moleculaire de Montpellier-Centre National de la Recherche Scientifique (CNRS), 34033 Montpellier Cedex 01, France.
Houser-Scott
F
F
Kendall
A
A
Singer
R H
RH
Engelke
D R
DR
D001483:Base Sequence; D002466:Cell Nucleolus / Q000378:metabolism*; D004722:Endoribonucleases / Q000378:metabolism; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000378:metabolism; D015347:RNA Probes; D012323:RNA Processing, Post-Transcriptional; D016337:RNA, Catalytic / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012343:RNA, Transfer / Q000096:biosynthesis* / Q000378:metabolism; D043262:Ribonuclease P; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*
D012322:RNA Precursors; D015347:RNA Probes; D016337:RNA, Catalytic; D012331:RNA, Fungal; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D004722:Endoribonucleases; D043262:Ribonuclease P