Induction of CPP32-like activity and inhibition of interleukin 1beta converting enzyme activity in the liver of a mouse concanavalin A-induced hepatitis model.
The objective of the present study was to determine the effects of concanavalin A (Con A) administration on the interleukin 1beta converting enzyme (ICE) activity and CPP32-like activity in mouse liver. Treatment with Con A (0.2 mg/mouse, i.v.) caused an elevated plasma alanine aminotransferase (ALT) level at 8 hr after Con A injection. ICE activity was decreased at 8 and 24 hr after Con A treatment. In contrast, CPP32-like activity was increased at 24 hr after Con A injection. Since CPP32-like activity was induced after ALT had increased, the induction of CPP32-like activity may not be involved in Con A-induced hepatitis.
Research Laboratories, Nippon Chemiphar Co., Ltd., Misato, Saitama, Japan.
T
T
Okamoto
T
T
Kobayashi
N
N
Tsuzuike
K
K
Hara
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000818:Animals; D020170:Caspase 1 / Q000187:drug effects*; D053148:Caspase 3; D020169:Caspases / Q000096:biosynthesis*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology*; D003208:Concanavalin A / Q000633:toxicity*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004195:Disease Models, Animal; D004790:Enzyme Induction; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C
D000590:Amino Acid Chloromethyl Ketones; D015853:Cysteine Proteinase Inhibitors; C096713:benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; D003208:Concanavalin A; C505409:Casp3 protein, mouse; D053148:Caspase 3; D020169:Caspases; D020170:Caspase 1