Antilactoferrin antibodies in autoimmune liver diseases.
OBJECTIVE
Lactoferrin, an immunoregulatory protein in mucosal secretions, is one of the target antigens to perinuclear antineutrophil cytoplasmic antibodies (P-ANCAs). Circulating lactoferrin is cleared in the liver, but little is known about the implication of lactoferrin in hepatic inflammation. To evaluate the implication of immunological response to lactoferrin, we examined antilactoferrin antibodies in autoimmune liver diseases.


METHODS
Fourteen patients with primary biliary cirrhosis (PBC), 14 with autoimmune hepatitis (AIH), five with autoimmune cholangitis (AIC), six with chronic hepatitis C, and five with chronic hepatitis B were studied. We evaluated autoantibodies to lactoferrin in the sera of the patients by the Western Immunoblotting method.


RESULTS
Sera of five of the 14 patients (35.7%) with PBC, four of the 14 patients (28.6%) with AIH, and five of the five patients (100%) with AIC contained autoantibodies to human lactoferrin, but none with hepatitis B or C had them. The higher prevalence of serum antibodies to human lactoferrin was shown to be higher in patients with AIC than with hepatitis B (p < 0.01), hepatitis C (p < 0.01), PBC (p < 0.05), and AIH (p < 0.05).


CONCLUSIONS
Lactoferrin located in bile ducts and liver cells is one of the candidates of target antigens in autoimmune liver diseases, especially in AIC.
Department of Gastroenterology, Tenri Hospital, Kyoto, Japan.
M
M
Ohana
K
K
Okazaki
K
K
Hajiro
K
K
Uchida
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D001327:Autoimmune Diseases / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods / Q000706:statistics & numerical data; D016009:Chi-Square Distribution; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods / Q000706:statistics & numerical data; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000276:immunology*; D008107:Liver Diseases / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D018709:Statistics, Nonparametric
D001323:Autoantibodies; D007781:Lactoferrin