Seizure activity induces PIM-1 expression in brain.
We recently identified KID-1, a previously undescribed protein kinase induced by depolarization in PC12 cells and brain (Feldman et al., 1998). KID-1 shares a high degree of sequence homology with PIM-1, a proto-oncogene previously reported to be expressed in hematopoietic and germ cells. We examined PIM-1 expression in stimulated PC12 cells, brains of kainic acid-treated rats, and a number of tissues from untreated rats. We now report that forskolin, but not depolarization or growth factors, induces PIM-1 expression in PC12 cells. PIM-1 is an immediate early gene induced in response to forskolin stimulation. We detect PIM-1 mRNA in a number of unstimulated tissues and at low levels in unstimulated brain. Systemic kainic acid administration to adult rats induces PIM-1 expression in the dentate gyrus region of the hippocampus.
Department of Pediatrics, University of California-Los Angeles, Center for the Health Sciences, USA.
J D
JD
Feldman
L
L
Vician
M
M
Crispino
G
G
Tocco
M
M
Baudry
H R
HR
Herschman
D000818:Animals; D001921:Brain / Q000378:metabolism*; D005576:Colforsin / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007608:Kainic Acid; D008297:Male; D009414:Nerve Growth Factors / Q000494:pharmacology; D009928:Organ Specificity; D016716:PC12 Cells; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic
D009414:Nerve Growth Factors; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D005576:Colforsin; D003513:Cycloheximide; C496637:Pim1 protein, rat; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1; D007608:Kainic Acid