[Radiosensitivity of Saccharomyces yeasts and srm genes: effects of srm1 and srm5 mutations].
The presence in the cell genotype of srm1 and srm5 (cdc28-srm) mutations decreasing the spontaneous rho- mutability was shown to have no effect on the rates of spontaneous nuclear gene mutations and gamma-ray-induced mitotic recombination. Mutation cdc28-srm exerts a marked effect on cell sensitivity to the lethal action of ionizing radiation and on the appearance of homoplasmic segregants generated from heteroplasmic diploids. Additive interactions between mutations cdc28-srm and each of the rad6 and rad52 mutations were revealed by an analysis of double mutants with respect to their sensitivity to radiation. Mutation rad9 was epistatic with mutation cdc28-srm. These data agree with the idea that the p34CDC28 gene product is a target for the RAD9-dependent feedback control operating at the cell cycle checkpoints (checkpoint control) and ensuring an additional amount of time for premitotic repair of chromosomal DNA damage.
Koltovaia
N A
NA
Department of Radiation and Radiological Research, Joint Institute for Nuclear Research, Dubna, Russia.
Karviga
T D
TD
Liubimova
K A
KA
Maĭorova
E S
ES
Arman
I P
IP
Devin
A B
AB
D000483:Alleles; D017489:CDC28 Protein Kinase, S cerevisiae / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D020662:Guanine Nucleotide Exchange Factors; D008025:Ligases / Q000235:genetics; D008938:Mitosis; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D051721:Rad52 DNA Repair and Recombination Protein; D011836:Radiation Tolerance / Q000235:genetics*; D011995:Recombination, Genetic; D012440:Saccharomyces / Q000235:genetics / Q000528:radiation effects*; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D009687:Nuclear Proteins; D051721:Rad52 DNA Repair and Recombination Protein; C067050:SRM1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D044763:Ubiquitin-Conjugating Enzymes; D017489:CDC28 Protein Kinase, S cerevisiae; D008025:Ligases