The meiosis-specific Hop2 protein of S. cerevisiae ensures synapsis between homologous chromosomes.
The hop2 mutant of S. cerevisiae displays a novel phenotype: meiotic chromosomes form nearly wild-type amounts of synaptonemal complex, but most chromosomes are engaged in synapsis with nonhomologous partners. The meiosis-specific Hop2 protein localizes to chromosomes prior to and during synapsis and in the absence of the double-strand breaks that initiate recombination. hop2 strains sustain a wild-type level of meiotic double-strand breaks, but these breaks remain unrepaired. The hop2 mutant arrests at the pachytene stage of meiotic prophase with the RecA-like protein Dmc1 located at numerous sites along synapsed chromosomes. We propose that the Hop2 protein functions to prevent synapsis between nonhomologous chromosomes.
Leu
J Y
JY
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520-8103, USA.
Chua
P R
PR
Roeder
G S
GS
D000595:Amino Acid Sequence; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002875:Chromosomes / Q000235:genetics*; D003001:Cloning, Molecular; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D005785:Gene Conversion; D015966:Gene Expression Regulation, Fungal; D008540:Meiosis / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013573:Synaptonemal Complex / Q000235:genetics
D018797:Cell Cycle Proteins; C513033:DMC1 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C113830:HOP2 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins