In vivo transduction with shiga toxin 1-encoding phage.
To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.
Acheson
D W
DW
Division of Geographic Medicine and Infectious Diseases and Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts, USA.
Reidl
J
J
Zhang
X
X
Keusch
G T
GT
Mekalanos
J J
JJ
Waldor
M K
MK
D000668:Ampicillin Resistance; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D007422:Intestines; D051379:Mice; D016254:Mutagenesis, Insertional; D022622:Shiga Toxin 1; D014161:Transduction, Genetic
D001427:Bacterial Toxins; D022622:Shiga Toxin 1