Elevation of mouse liver glutathione level by low-dose gamma-ray irradiation and its effect on CCl4-induced liver damage.
We examined the elevation of glutathione (GSH) levels in the liver of C57BL/6 female mice after low-dose r-ray irradiation and its inhibitory effect on CClI4-induced liver damage. The liver GSH level increased soon after irradiation with 50 cGy of gamma-rays, reached a maximum at around 12 post-treatment, and returned almost to the control level by 24 h. The activities of glutathione reductase, and glutathione peroxidase also showed the same pattern of change, while the activity of gamma-glutamylcysteine synthetase showed a gradual increase up to 24 h. The effect of pre-irradiation on CCl4-induced liver damage was also investigated. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in serum were markedly increased 12 h post-treatment with CCl4. Both increases were significantly suppressed by a single low-dose pre-irradiation. Malondialdehyde, a marker of lipidperoxidation, was also greatly elevated after CCl4 treatment, and its increase was suppressed by irradiation. These results suggest low-dose gamma-ray irradiation might be effective for the prevention of and/or therapy of various reactive oxygen species-related diseases including cancer.
Research Institute for Biological Sciences, Science University of Tokyo, Chiba, Japan.
S
S
Kojima
O
O
Matsuki
T
T
Nomura
A
A
Kubodera
K
K
Yamaoka
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005720:Gamma Rays; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism / Q000528:radiation effects; D005978:Glutathione / Q000378:metabolism* / Q000528:radiation effects; D005979:Glutathione Peroxidase / Q000378:metabolism / Q000528:radiation effects; D005980:Glutathione Reductase / Q000378:metabolism / Q000528:radiation effects; D007700:Kinetics; D008099:Liver / Q000378:metabolism / Q000473:pathology / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D013997:Time Factors
D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione