The gene encoding the granulocyte colony-stimulating factor receptor is a target for deregulation in pre-B ALL by the t(1;19)-specific oncoprotein E2A-Pbx1.
Approximately 25-30% of childhood pre-B cell acute lymphoblastic leukemias (pre-B ALL) is characterized by the presence of a (1;19)(q23;p13.3) translocation. The presence of this translocation is generally accompanied by a poor prognosis. The chimeric gene resulting from this chromosomal rearrangement encodes a hybrid transcription factor, E2A-Pbx1. In an attempt to delineate the genetic cascade initiated by E2A-Pbx1, we sought to identify genes that are deregulated by this transcription factor in t(1;19) pre-B ALL. We show here that the gene encoding the granulocyte colony-stimulating factor receptor (G-CSFr) is specifically upregulated in pre-B cells expressing E2A-Pbx1. G-CSFr is also expressed in cell lines established from t(1;19) pre-B cell leukemia and on primary t(1;19) tumor cells, but not on control cells. These data indicate that G-CSFr gene is a target for deregulation by E2A-Pbx1.
Academic Hospital Utrecht, Department of Immunology, The Netherlands.
W B
WB
de Lau
J
J
Hurenkamp
P
P
Berendes
I P
IP
Touw
H C
HC
Clevers
M A
MA
van Dijk
D001402:B-Lymphocytes; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000378:metabolism; D002878:Chromosomes, Human, Pair 1; D002888:Chromosomes, Human, Pair 19; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D015514:Oncogene Proteins, Fusion / Q000235:genetics / Q000378:metabolism*; D016188:Receptors, Granulocyte Colony-Stimulating Factor / Q000235:genetics* / Q000502:physiology; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured
D018398:Homeodomain Proteins; D015514:Oncogene Proteins, Fusion; D016188:Receptors, Granulocyte Colony-Stimulating Factor; C089847:E2A-Pbx1 fusion protein