Effects of wine on plasma fibrinolytic and coagulation systems.
The in-vitro effects of citrus, red and white wines on human plasma fibrinolytic enzymes were compared. When citrus wine was added to plasmin, H-D-Val-Leu-Lys-pNA (S-2251) amidolysis was not changed. Although, it was significantly inhibited when red and white wines were added. The pyro-Glu-Gly-Arg-pNA (S-2444) amidolysis of the plasminogen activator urokinase was inhibited by all types of wine. Since the same amount of Et-OH as the wine's content did not effect on these inhibitions, it can be assumed to be due to other unknown substances in wines besides alcohol. In in-vivo test of 20 normal volunteers (ages 20-52) with various wines equivalent to 30-60 ml of alcohol content, the coagulation parameters of gamma and kappa values on thromboelastography were not so much changed after 1-2 hrs of the application. Also, there was not much difference of the fibrinolytic parameters in terms of the plasma euglobulin clotlysis time, S-2444 amidolysis, and Ma values on thromboelastography. However, the protein concentration and the enzyme activity of the urokinase-like plasminogen activator, which were extracted from the plasma of volunteers who had the citrus wine 1 hr before the test were higher than twice of the before control. The main molecular form of this enzyme was proved to have a molecular weight of about 30,000 by zymography. It is concluded that not only wines induce the endogenous plasminogen activator just like other alcoholic beverages but also wines contain a fibrinolysis inhibitor which works directly to the enzyme dissolubility. This finding warns that extremely complex results can be expected depending on the test method.
Department of Physiology, Miyazaki Medical College, Japan.
H
H
Sumi
Y
Y
Kozaki
C
C
Yatagai
H
H
Hamada
D000328:Adult; D001777:Blood Coagulation; D005260:Female; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D009842:Oligopeptides / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000097:blood; D014920:Wine
D009842:Oligopeptides; C018788:valyl-leucyl-lysine 4-nitroanilide; C028190:5-oxo-prolyl-glycyl-arginine-4-nitroanilide; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator