A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay.
Telomerase activity has been observed in a high proportion of specimens from a wide variety of human malignant tumours, but not in non-neoplastic tissues. Measurement of telomerase activity is a possible diagnostic maker for malignant tumours. A non-isotopic telomeric repeat amplification protocol (TRAP) silver staining method for the detection of telomerase activity in human bone tumour tissues is described. The TRAP-silver staining method was quick, safe, and effective, and can be used as a routine diagnostic method for the detection of telomerase activity in bone tumours.
Department of Pathology, Sun Yat-Sen University of Medical Sciences, Guangzhou, People's Republic of China.
J M
JM
Wen
L B
LB
Sun
M
M
Zhang
M H
MH
Zheng
D014408:Biomarkers, Tumor / Q000378:metabolism*; D001859:Bone Neoplasms / Q000201:enzymology*; D004996:Ethidium; D006801:Humans; D016133:Polymerase Chain Reaction; D016622:Silver Staining / Q000379:methods*; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere
D014408:Biomarkers, Tumor; D019098:Telomerase; D004996:Ethidium