[Genotyping of Shigella flexneri 2a strains isolated from patients with acute dysentery in St. Petersburg].
Polymerase chain reaction with universal primers (UP-PCR) was used for the genotyping of 76 S.flexneri 2a cultures isolated from patients with acute dysentery in infectious and psychoneurological hospitals of St. Petersburg. 9 types were determined, and each of them included cultures with identical UP-PCR patterns. The population of the infective agent was more heterogeneous in psychoneurological hospitals where the change of types was registered in May-September 1995. Some of these types were probably epidemic strains. UP-PCR was found to be a promising method increasing the efficiency of traditional epidemiological analysis.
State Pediatric Medical Academy, Pasteur Research Institute of Epidemiology and Microbiology, St. Petersburg, Russia.
E L
EL
Stavitskaia
O V
OV
Narvskaia
I V
IV
Mokrousov
A T
AT
Zhurkin
F S
FS
Noskov
D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004405:Dysentery, Bacillary / Q000453:epidemiology / Q000235:genetics* / Q000382:microbiology; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012426:Russia / Q000453:epidemiology; D012763:Shigella flexneri / Q000235:genetics* / Q000302:isolation & purification