Structural organization of the human selenium-dependent phospholipid hydroperoxide glutathione peroxidase gene (GPX4): chromosomal localization to 19p13.3.
The primary structure of human selenium-dependent phospholipid hydroperoxide glutathione peroxidase (GPX4) was determined by genomic cloning. The gene structure of GPX4 spans only 2.8 kb and consists of 7 exons. The coding sequence resides on all 7 exons, and the mitochondrial leader sequence is contained entirely within the first exon. The selenocysteine coding nucleotide resides on the third exon. The introns all commenced with the consensus nucleotide sequence GTR and ended with the consensus nucleotide sequence YAG. Analysis of the GPX4 gene sequence identified a potential alternative tissue-specific first exon. Chromosomal FISH studies placed the GPX4 gene at 19p13.3 location, and downstream of the 23 k-Da polypeptide DNA-directed RNA polymerase gene.
Kelner
M J
MJ
Department of Pathology, University of California, San Diego 92103-8320, USA. mkelner@ucsd.edu
Montoya
M A
MA
D002874:Chromosome Mapping; D002888:Chromosomes, Human, Pair 19; D015894:Genome, Human; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase
D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D005979:Glutathione Peroxidase