Microjet impingement followed by scanning electron microscopy as a qualitative technique to compare cellular adhesion to various biomaterials.
Adhesion of cells to biomaterial surfaces is one of the major factors which mediates their biocompatibility. Quantitative or qualitative cell adhesion measurements would be useful for screening new implant materials. Microjet impingement has been evaluated by scanning electron microscopy, to determine to what extent it measures cell adhesion. The shear forces of the impingement, on the materials tested here, are seen to be greater than the cohesive strength of the cells in the impinged area, causing their rupture. The cell bodies are removed during impingement, leaving the sites of adhesion and other cellular material behind. Thus the method is shown not to provide quantification of cell adhesion forces for the metals and culture plastic tested. It is suggested that with highly adherent biomaterials, the distribution and patterns of these adhesion sites could be used for qualitative comparisons for screening of implant surfaces.
AO/ASIF Research Institute, Davos, Switzerland.
R G
RG
Richards
I
I
ap Gwynn
K J
KJ
Bundy
B A
BA
Rahn
D016475:3T3 Cells / Q000166:cytology* / Q000648:ultrastructure*; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D003599:Cytoskeleton / Q000648:ultrastructure; D008422:Materials Testing / Q000379:methods*; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011554:Pseudopodia / Q000648:ultrastructure; D013232:Steel; D013314:Stress, Mechanical; D016707:Tissue Fixation; D014025:Titanium
D013232:Steel; D014025:Titanium