A gloverin-like antibacterial protein is synthesized in Helicoverpa armigera following bacterial challenge.
A bacteria inducible antibacterial protein, P2, was isolated from the old world bollworm Helicoverpa armigera. Fifth-instar larvae were injected with live Escherichia coli NCTC 8196. P2 was isolated by HPLC using reversed-phase and size-exclusion columns. In addition, P2 was isolated by an alternative method of sequential cation-exchange and reversed-phase HPLC. The structure of P2 was determined by N-terminal Edman degradation and mass spectrometry. P2 had similar mass (14.1 kDa) structure and activity to gloverin, an inducible glycine-rich antibacterial protein isolated from Hyalophora gloveri [Axén, A.; Carlsson, A.; Engström, A.; Bennich, H. Eur. J. Biochem. 247:614-619; 1997]. At the N-terminus P2 had approximately 60% identity with gloverin. P2 is basic, heat stable, and displayed rapid antibacterial action. P2 was active against the Gram-negative bacteria tested and was inactive against the Gram-positive bacteria, Candida albicans, a bovine turbinate cell line, and pestivirus.
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia. jmackint@rna.bio.mq.edu.au
J A
JA
Mackintosh
A A
AA
Gooley
P H
PH
Karuso
A J
AJ
Beattie
D R
DR
Jardine
D A
DA
Veal
D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000502:physiology*; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D006458:Hemolymph / Q000737:chemistry; D036341:Intercellular Signaling Peptides and Proteins; D007915:Lepidoptera / Q000378:metabolism / Q000382:microbiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010576:Pestivirus / Q000187:drug effects; D014176:Protein Biosynthesis; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid
D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D036341:Intercellular Signaling Peptides and Proteins; D011506:Proteins; C107617:gloverin