A novel angiotensin II binding site in murine tissues.
A novel binding site for angiotensin II has been identified in certain murine tissues. This site, denoted ATm, is distinct from both the AT1 and AT2 sites, as well as the various atypical sites that have been described. The site has a low affinity for angiotensin II (100 nM) and is not blocked by losartan, PD123177 or saralasin. The site shows structural specificity for angiotensin II since both angiotensin III and angiotensin II (1-7) failed to inhibit the binding. Numerous standard drugs, including various receptor blockers, enzyme inhibitors and therapeutic agents, showed no affinity for the site. In murine tissues the site is associated with active tissue generation, as found in spleen, placenta and growing tumors, but its occurrence appears to be rare outside of the mouse.
Herblin
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WF
Cardiovascular Diseases Research, The DuPont Merck Pharmaceutical Co., Wilmington, DE 20037, USA.
Barbera
F
F
Diamond
S
S
Gibson
R E
RE
Timmermans
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P
D000595:Amino Acid Sequence; D000804:Angiotensin II / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D019808:Losartan / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D011725:Pyridines / Q000494:pharmacology; D012504:Saralasin / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D007093:Imidazoles; D011725:Pyridines; D000804:Angiotensin II; C066253:PD 123177; D012504:Saralasin; D019808:Losartan