DNA analysis of a grandfather-father-son relationship from 300-year-old remains of the Date clan in Japan.
The grandfather-father-son relationship of the first three lords of the Date clan in Japan was ascertained by HLA-DNA sequencing analysis. From their hairs and dried lung tissue found in ca. 300-year-old remains, DNA was extracted with usual phenol-chloroform method followed by purification with cetyltrimethylammonium bromide (CTAB). Two HLA class II genes, HLA-DQA1 and -DPB1, were amplified by seminested, or dual/triple PCR. The PCR products were cloned and analyzed by automated sequencing. Since the three lords shared a haplotype of DQA1*0301-DPB1*0402, we concluded that there is no inconsistency in their lineage. This is the first case of biological evidence for a historical Samurai family relationship in the 17-18th centuries.
Uchihi
R
R
Department of Legal Medicine, Nagoya University School of Medicine, Japan.
Yamamoto
T
T
Nozawa
H
H
Tamaki
K
K
Ozawa
T
T
Yamada
T K
TK
Katsumata
Y
Y
D001483:Base Sequence; D004247:DNA / Q000235:genetics* / Q000266:history; D005802:Genes, MHC Class II / Q000235:genetics*; D005838:Genotype; D006682:HLA-DP Antigens / Q000235:genetics; D059868:HLA-DP beta-Chains; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006197:Hair / Q000737:chemistry; D049670:History, 17th Century; D049671:History, 18th Century; D006801:Humans; D007564:Japan; D008168:Lung / Q000737:chemistry; D008297:Male; D008969:Molecular Sequence Data; D010334:Paternity; D016133:Polymerase Chain Reaction
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D004247:DNA