A transposition-induced mutant of Nostoc ellipsosporum implicates an arginine-biosynthetic gene in the formation of cyanophycin granules and of functional heterocysts and akinetes.
In strain NE1 of Tn5-1058-mutagenized Nostoc ellipsosporum, the transposon was found within a gene whose translation product is similar in amino acid sequence to the arginine-biosynthetic protein N-acetylglutamate semialdehyde dehydrogenase encoded by argC of Bacillus subtilis. The argC reported from Anabaena sp. strain PCC 7120 hybridized to a sequence different from the one interrupted by the transposon in NE1. The newly identified gene from N. ellipsosporum was denoted argL. The argL mutation renders certain processes in strain NE1 conditionally dependent on provision of L-arginine. Heterocysts and apparent akinetes that formed in the absence of added L-arginine failed to fix dinitrogen or to germinate, respectively, and lacked granules of cyanophycin, composed of copolymers of arginine and aspartic acid. However, apparent akinetes that differentiated upon growth of the mutant in the presence of L-arginine plus nitrate formed cyanophycin granules and could regenerate a new culture.
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid28049 MadridSpain.
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Leganés
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid28049 MadridSpain.
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Fernández-Piñas
MSU-DOE Plant Research Laboratory, Michigan State UniversityEast Lansing, Ml 48824USA.
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Wolk
D000445:Aldehyde Oxidoreductases; D000595:Amino Acid Sequence; D001120:Arginine / Q000235:genetics*; D001224:Aspartic Acid / Q000032:analysis; D001426:Bacterial Proteins / Q000235:genetics*; D015139:Blotting, Southern; D000458:Cyanobacteria / Q000166:cytology* / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D004247:DNA; D004251:DNA Transposable Elements / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000494:pharmacology; D009591:Nitrogenase / Q000032:analysis; D010940:Plant Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010940:Plant Proteins; C024250:cyanophycin; D001224:Aspartic Acid; D004247:DNA; D001120:Arginine; D009591:Nitrogenase; D000445:Aldehyde Oxidoreductases; C089846:argC protein, Bacteria; D009584:Nitrogen