Aqueous two-phase systems: a novel approach for the separation of proteose peptones.
Poly(ethylene glycol) and dextran aqueous two-phase systemis (ATPS) were developed to facilitate the separation of components of the proteose peptone fraction of bovine milk, which are mostly large casein derived peptides or glycoproteins. These have proved difficult to purify using conventional chromatographic procedures. ATPS exploit differences in hydrophobicity, size and ionic properties of the proteose peptones with a view to developing methods for future large scale preparations of the individual components of this whey protein fraction.
Isgrove
F H
FH
Food Chemistry Group, School of Food and Consumer Science, University of Wales Institute, Cardiff, UK.
Williams
R J
RJ
Niven
G W
GW
Andrews
A T
AT
D000595:Amino Acid Sequence; D002364:Caseins / Q000737:chemistry / Q000302:isolation & purification; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D010461:Peptones / Q000737:chemistry / Q000302:isolation & purification*; D014867:Water
D002364:Caseins; D010446:Peptide Fragments; D010461:Peptones; C018135:proteose-peptone; D014867:Water