Clinical application of hepatitis C virus core protein in early diagnosis of acute hepatitis C.
A fluorescence enzyme immunoassay (FEIA) for the quantitative measurement of hepatitis C virus (HCV) core protein has recently been developed. In this study, we studied the clinical usefulness of this measurement in patients with acute hepatitis C. Eighteen patients with post-transfusion acute hepatitis C were enrolled in the study; 5 patients showed resolution of hepatitis with disappearance of HCV viremia, while the remaining 13 patients did not. A second generation HCV antibody, HCV RNA, and HCV core protein were measured in serial serum samples taken within 1 month of the onset of acute hepatitis and 3, 6, 12, 24, and 36 months after onset. Within the first month after disease onset, the positivity rates of HCV RNA (100%; P = 0.0014) and HCV core protein (89%; P = 0.0300) were both significantly higher than that of HCV antibody (56%). Six months after disease onset, the positivity rate of HCV antibody had increased, to 100%, and the positivity rates of HCV RNA and HCV core protein began to decrease. HCV core protein levels did not differ between patients with resolved and unresolved disease in the first month after disease onset. These findings indicate that FEIA, a simple assay, for the measurement of HCV core protein was useful for the early diagnosis of acute hepatitis C.
Kobayashi
M
M
Second Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Tanaka
E
E
Matsumoto
A
A
Yoshizawa
K
K
Imai
H
H
Sodeyama
T
T
Kiyosawa
K
K
D000208:Acute Disease; D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D005455:Fluorescent Antibody Technique; D016174:Hepacivirus / Q000302:isolation & purification*; D006526:Hepatitis C / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D065227:Transfusion Reaction; D014758:Viral Core Proteins / Q000097:blood*
D015415:Biomarkers; D018937:Hepatitis C Antibodies; D014758:Viral Core Proteins