Sequence analysis of the cupin gene family in Synechocystis PCC6803.
The recently described cupin superfamily of proteins includes the germin and germinlike proteins, of which the cereal oxalate oxidase is the best characterized. This superfamily also includes seed storage proteins, in addition to several microbial enzymes and proteins with unknown function. All these proteins are characterized by the conservation of two central motifs, usually containing two or three histidine residues presumed to be involved with metal binding in the catalytic active site. The present study on the coding regions of Synechocystis PCC6803 identifies a previously unknown group of 12 related cupins, each containing the characteristic two-motif signature. This group comprises 11 single-domain proteins, ranging in length from 104 to 289 residues, and includes two phosphomannose isomerases and two epimerases involved in cell wall synthesis, a member of the pirin group of nuclear proteins, a possible transcriptional regulator, and a close relative of a cytochrome c551 from Rhodococcus. Additionally, there is a duplicated, two-domain protein that has close similarity to an oxalate decarboxylase from the fungus Collybia velutipes and that is a putative progenitor of the storage proteins of land plants.
Department of Agricultural Botany, School of Plant Sciences, University of Reading, UK.
J M
JM
Dunwell
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D002262:Carboxy-Lyases / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D000458:Cyanobacteria / Q000235:genetics*; D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics; D005798:Genes, Bacterial; D006023:Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins / Q000235:genetics; D010088:Oxidoreductases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D002352:Carrier Proteins; D005656:Fungal Proteins; D006023:Glycoproteins; D009687:Nuclear Proteins; D010940:Plant Proteins; C058814:germin; D010088:Oxidoreductases; C020598:oxalate oxidase; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase