Evaluation of intra- and interindividual variation of urinary 1-hydroxypyrene, a biomarker of exposure to polycyclic aromatic hydrocarbons.
The study was performed on 30, 8-year-old children living in an industrial town of the Upper Silesia region. Morning urine samples were collected on 6 consecutive days. Intraindividual variation of urinary 1-hydroxypyrene concentrations, calculated as a coefficient of variance (CV), ranged from 14 to 109% whereas inter-individual variation ranged from 69 to 109%. Three-way analysis of variance disclosed a significant effect of sex, exposure to environmental tobacco smoke and day of examination on 1-hydroxypyrene concentrations not corrected for creatinine. The appropriate sample size for population studies and the minimum number of observations for the individual assessment of environmental exposure to PAHs calculated on the basis of inter- and intraindividual variability of 1-hydroxypyrene concentrations in urine amounted to 164 and 99, respectively. Urinary 1-hydroxypyrene may be considered a good indicator of environmental exposure to PAHs at the group level.
Institute of Occupational Medicine and Environmental Health, Sosnowiec, Poland.
E
E
Siwińska
D
D
Mielzyńska
E
E
Smolik
A
A
Bubak
J
J
Kwapuliński
D015415:Biomarkers; D002648:Child; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D006841:Hydrocarbons, Aromatic / Q000032:analysis*; D008297:Male; D009153:Mutagens / Q000032:analysis*; D011044:Poland; D011083:Polycyclic Compounds / Q000032:analysis*; D011721:Pyrenes / Q000032:analysis*; D015203:Reproducibility of Results; D014556:Urine / Q000737:chemistry
D015415:Biomarkers; D006841:Hydrocarbons, Aromatic; D009153:Mutagens; D011083:Polycyclic Compounds; D011721:Pyrenes; C033146:1-hydroxypyrene