Cloning of a mammalian elk potassium channel gene and EAG mRNA distribution in rat sympathetic ganglia.
1. Three new members of the EAG potassium channel gene family were identified in rat and the complete coding sequence of one of these genes (elk1) was determined by cDNA cloning. 2. The elk1 gene, when expressed in Xenopus oocytes, encodes a slowly activating and slowly deactivating potassium channel. 3. The elk1 gene is expressed in sympathetic ganglia and is also expressed in sciatic nerve. 4. Six of the seven known EAG genes were found to be expressed in rat sympathetic ganglia, suggesting an important functional role for these channels in the sympathetic nervous system.
Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
W
W
Shi
H S
HS
Wang
Z
Z
Pan
R S
RS
Wymore
I S
IS
Cohen
D
D
McKinnon
J E
JE
Dixon
D000818:Animals; D001923:Brain Chemistry / Q000502:physiology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000302:isolation & purification; D004268:DNA-Binding Proteins; D051638:Ether-A-Go-Go Potassium Channels; D005728:Ganglia, Sympathetic / Q000737:chemistry*; D015870:Gene Expression / Q000502:physiology; D008322:Mammals; D008969:Molecular Sequence Data; D015221:Potassium Channels / Q000235:genetics*; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012584:Sciatic Nerve / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014982:Xenopus laevis; D050807:ets-Domain Protein Elk-1
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C494114:Elk1 protein, rat; D051638:Ether-A-Go-Go Potassium Channels; D015221:Potassium Channels; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; D050807:ets-Domain Protein Elk-1