Role of IQGAP1, a target of the small GTPases Cdc42 and Rac1, in regulation of E-cadherin- mediated cell-cell adhesion.
The small guanosine triphosphatases (GTPases) Cdc42 and Rac1 regulate E-cadherin-mediated cell-cell adhesion. IQGAP1, a target of Cdc42 and Rac1, was localized with E-cadherin and beta-catenin at sites of cell-cell contact in mouse L fibroblasts expressing E-cadherin (EL cells), and interacted with E-cadherin and beta-catenin both in vivo and in vitro. IQGAP1 induced the dissociation of alpha-catenin from a cadherin-catenin complex in vitro and in vivo. Overexpression of IQGAP1 in EL cells, but not in L cells expressing an E-cadherin-alpha-catenin chimeric protein, resulted in a decrease in E-cadherin-mediated cell-cell adhesive activity. Thus, IQGAP1, acting downstream of Cdc42 and Rac1, appears to regulate cell-cell adhesion through the cadherin-catenin pathway.
Division of Signal Transduction, Nara Institute of Science and Technology, Ikoma 630-0101, Japan.
S
S
Kuroda
M
M
Fukata
M
M
Nakagawa
K
K
Fujii
T
T
Nakamura
T
T
Ookubo
I
I
Izawa
T
T
Nagase
N
N
Nomura
H
H
Tani
I
I
Shoji
Y
Y
Matsuura
S
S
Yonehara
K
K
Kaibuchi
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D003598:Cytoskeletal Proteins / Q000378:metabolism; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020690:GTPase-Activating Proteins; D007739:L Cells; D051379:Mice; D009154:Mutation; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins
C495267:CTNNB1 protein, mouse; D015820:Cadherins; D018797:Cell Cycle Proteins; C495272:Ctnna1 protein, mouse; D003598:Cytoskeletal Proteins; D020690:GTPase-Activating Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins; D020764:cdc42 GTP-Binding Protein; D020744:rac GTP-Binding Proteins