Replacement of loop II of VP1 of the DA strain with loop II of the GDVII strain of Theiler's murine encephalomyelitis virus alters neurovirulence, viral persistence, and demyelination.
Theiler's murine encephalomyelitis viruses, which are murine picornaviruses, can cause central nervous system inflammatory disease. To study the role of loop II in capsid protein VP1, two mutant viruses of strain DA in which DA loop II amino acids were replaced with strain GDVII amino acids were constructed. Infection of mice with the two mutant viruses led to dramatically different patterns of disease.
Department of Neurology and Pathology, University of Utah, Salt Lake City, Utah 84132, USA.
Y
Y
Wada
I J
IJ
McCright
F G
FG
Whitby
I
I
Tsunoda
R S
RS
Fujinami
D000818:Animals; D001921:Brain / Q000821:virology; D002213:Capsid / Q000737:chemistry / Q000235:genetics* / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D006224:Cricetinae; D003711:Demyelinating Diseases / Q000473:pathology*; D051379:Mice; D016297:Mutagenesis, Site-Directed; D011051:Poliomyelitis / Q000473:pathology* / Q000821:virology; D011487:Protein Conformation; D013116:Spinal Cord / Q000473:pathology; D017945:Theilovirus / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D014774:Virulence; D017735:Virus Latency
D036022:Capsid Proteins; C442551:VP1 protein, Theilovirus