Quantitative analysis of non-steroidal anti-inflammatory drugs by capillary zone electrophoresis.
The potential utility of capillary zone electrophoresis (CZE) for the separation and quantitative determination of some non-steroidal anti-inflammatory drugs (NSAIDs) was investigated. The influence of different parameters on migration times, peak symmetry, efficiency and resolution was studied; these parameters included the nature and concentration of the anionic and cationic components of the separation buffer. A buffer consisting of 75 mM glycine adjusted to pH 9.1 with triethanolamine was found to provide a very efficient and stable electrophoretic system for the CZE analysis of NSAIDs, giving RSD values of about 0.1 and 0.5% for the within-day reproducibility of migration times and peak areas, respectively at a concentration of 25 micrograms ml-1 (n = 5). Response was linear from 2-100 micrograms ml-1 for both sulindac and tiaprofenic acid, for which the LOQ values were 2.8 and 1.9 micrograms ml-1, respectively, using UV detection at 280 nm. Accuracy for each drug was 102-103%.
Laboratory of Drug Analysis, University of Liège, Belgium.
I
I
Bechet
M
M
Fillet
P
P
Hubert
J
J
Crommen
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis* / Q000302:isolation & purification; D002021:Buffers; D002412:Cations; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D011422:Propionates / Q000032:analysis / Q000302:isolation & purification; D012996:Solutions; D013467:Sulindac / Q000032:analysis / Q000302:isolation & purification
D000894:Anti-Inflammatory Agents, Non-Steroidal; D002021:Buffers; D002412:Cations; D011422:Propionates; D012996:Solutions; D013467:Sulindac; C021270:tiaprofenic acid