Inhibitory effects of 5-S-GAD on phosphorylation of V-SRC and BCR-ABL tyrosine kinase.
We examined the inhibitory effects of N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine (5-S-GAD), a novel antibacterial substance from the immunized adult Sarcophaga peregrina (flesh fly), on protein phosphorylation using immune complexes of protein tyrosine kinases (PTKs) with anti-PTKs monoclonal antibody. We found that 5-S-GAD directly inhibited not only tyrosine phosphorylation of PTK p60(v-src), but also tyrosine phosphorylation of PTK p210(BCR-ABL). The inhibitory potency of 5-S-GAD was comparable to that of radicicol and herbimycin A of PTK inhibitor.
Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
J Y
JY
Leem
H Y
HY
Park
H
H
Fukazawa
Y
Y
Uehara
S
S
Natori
D016475:3T3 Cells; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D004295:Dihydroxyphenylalanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D004175:Diptera / Q000737:chemistry; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D005978:Glutathione / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D051379:Mice; D015688:Oncogene Protein pp60(v-src) / Q000378:metabolism*; D010766:Phosphorylation; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured; D014443:Tyrosine / Q000378:metabolism
D000890:Anti-Infective Agents; D004791:Enzyme Inhibitors; C100496:N-beta-alanyl-5-S-glutathionyl-3,4-dihydroxyphenylalanine; D014443:Tyrosine; D004295:Dihydroxyphenylalanine; D011505:Protein-Tyrosine Kinases; D016044:Fusion Proteins, bcr-abl; D015688:Oncogene Protein pp60(v-src); D005978:Glutathione