The nirQ gene, which is required for denitrification of Pseudomonas aeruginosa, can activate the RubisCO from Pseudomonas hydrogenothermophila.
Two putative ATP-binding proteins encoded in the gene cluster for the Calvin cycle of Pseudomonas hydrogenothermophila (cbbQ) and for the denitrification of Pseudomonas aeruginosa (nirQ) have been found to be similar. The cbbQ gene has been shown to activate the RubisCO from P. hydrogenothermophila in E. coli. The nirQ was functionally substituted for cbbQ. The nirQ gene restored the anaerobic growth and the NOR activity of the nirQOP mutant of P. aeruginosa, while the cbbQ gene did not.
Hayashi
N R
NR
Department of Biotechnology, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Arai
H
H
Kodama
T
T
Igarashi
Y
Y
D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D001665:Binding Sites; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D009584:Nitrogen / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015183:Restriction Mapping; D012273:Ribulose-Bisphosphate Carboxylase / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D015534:Trans-Activators; C078738:nirQ protein, Pseudomonas; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; C034747:nitric-oxide reductase; D012273:Ribulose-Bisphosphate Carboxylase; D009584:Nitrogen