Biogenic amines in silage, apparent postruminal passage, and the relationship between biogenic amines and digestive function and intake by steers.
A 4 x 4 Latin square experiment was conducted to examine abomasal passage of biogenic amines in steers fed silage and their related effects on intake, digestibility, and digestive function. Thirty percent of the dry matter (DM) in the diets consisted of alfalfa forage, which was fed as either hay or silage. The DM from alfalfa silage DM was substituted at 0, 33, 67, and 100% for DM from alfalfa hay and was fed to four ruminally and abomasally cannulated steers. The roughage component of the diet constituted 50% of the DM and consisted of 60% alfalfa silage or hay and 40% tropical corn silage. The concentrate was composed mainly of ground corn. The concentrations of putrescine and cadaverine in abomasal digesta increased as alfalfa silage in the diet increased. Abomasal recovery of biogenic amines, a product of their concentration in abomasal digesta and the passage of DM through the abomasum, was negatively correlated with intake. Abomasal recovery of most amines was 5 to 20% of intake. Abomasal recovery of cadaverine was correlated with depressed intake. Total DM intake was reduced 8.3 to 25.8% as the proportion of alfalfa silage in the diet increased. Frequency of reticular contractions, intake, ruminal DM digestibility, ruminal outflow, volatile fatty acids, and total tract DM digestibility decreased in steers fed diets that contained more alfalfa silage. Ruminal fluid pH and NH3 concentration increased in steers fed more alfalfa silage; however, mass and the DM percentage of ruminal contents decreased linearly. Postprandial insulin concentrations were quadratically related to the proportion of alfalfa hay or silage in the diet. Intraruminal metabolism of biogenic amines is extensive based on the relatively low quantities recovered in abomasal digesta; however, the amounts recovered in abomasal digesta were related to intake depression and associated physiological effects.
Phuntsok
T
T
Department of Animal and Dairy Science, University of Georgia, Athens 30602, USA.
Froetschel
M A
MA
Amos
H E
HE
Zheng
M
M
Huang
Y W
YW
D000018:Abomasum / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002417:Cattle / Q000502:physiology*; D004063:Digestion; D004435:Eating; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005769:Gastrointestinal Motility; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000097:blood; D008297:Male; D000455:Medicago sativa; D011073:Polyamines / Q000008:administration & dosage / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D012417:Rumen / Q000378:metabolism*; D012820:Silage / Q000032:analysis*; D003313:Zea mays
D000596:Amino Acids; D001679:Biogenic Amines; D005232:Fatty Acids, Volatile; D007328:Insulin; D011073:Polyamines; D000644:Quaternary Ammonium Compounds