Detection of Aspergillus galactomannan: comparison of an enzyme-linked immunoassay and a europium-linked time-resolved fluoroimmunoassay.
With a view to improving the sensitivity of serological detection of Aspergillus galactomannan (GM), a europium-linked time-resolved fluoroimmunoassay was developed. This method was compared to an enzyme-linked immunosorbent assay using a peroxidase-conjugated detector antibody. No increase in the sensitivity of the detection of GM standards was seen with the europium-based fluoroimmunoassay.
Paugam
A
A
Laboratoire de Mycologie, Hôpital Cochin, Paris, France. andre.paugam@cch.ap-hop-paris.fr
Sarfati
J
J
Romieu
R
R
Viguier
M
M
Dupouy-Camet
J
J
Latgé
J P
JP
D000946:Antigens, Fungal / Q000032:analysis*; D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005063:Europium; D005455:Fluorescent Antibody Technique; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008351:Mannans / Q000032:analysis*; D012680:Sensitivity and Specificity
D000946:Antigens, Fungal; D008351:Mannans; C012990:galactomannan; D005063:Europium; D005690:Galactose