Analysis of the basal and inducible activities of the ICPO promoter of herpes simplex virus type 1.
Sequences from -420 to -70 from the ICPO transcriptional start site of herpes simplex virus type 1 are dispensable for reactivation from latency. A putative cAMP-response element (CRE) outside of this region was non-functional in both murine neuroblastoma (NB41A3) and rat pheochromocytoma (PC12) cells. Also, poor binding of cAMP-response element binding protein (CREB) was observed. Sequences from -95 to -37 are important for constitutive activity in NB41A3, PC12 and baby hamster kidney (BHK) cells. The TATA box and Sp1 site were also shown to be major contributors to constitutive activity. Finally, high constitutive activity of a deleted construct (-420 to -1) in NB41A3 and BHK cells suggests transcription initiates upstream of -420 in the absence of VP16. The implications of these observations regarding ICPO expression during the virus life-cycle are discussed.
Davido
D J
DJ
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA.
Leib
D A
DA
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D017781:Genes, Immediate-Early; D005814:Genes, Viral; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D017874:Immediate-Early Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D016716:PC12 Cells; D011401:Promoter Regions, Genetic; D051381:Rats; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000378:metabolism; D016385:TATA Box; D044767:Ubiquitin-Protein Ligases; D014775:Virus Activation / Q000235:genetics
D017362:Cyclic AMP Response Element-Binding Protein; D004279:DNA, Viral; D017874:Immediate-Early Proteins; D016329:Sp1 Transcription Factor; D044767:Ubiquitin-Protein Ligases; C051804:Vmw110 protein, Human herpesvirus 1