[Ways to decrease inhibition of the polymerase chain reaction by the components of the clinical specimens].
Inhibition of the polymerase chain reaction (PCR) by components of the analyzed specimen is an important problem in analysis of clinical material by this method. The inhibitory activity of clinical material can be decreased by additional purification of DNA by gel filtration on microcolumns. The method was developed using blood-containing specimens and tried on many clinical specimens. Commercial PCR kit for detecting Mycobacterium tuberculosis was used. The proposed method for DNA purification notably improves the efficacy of detecting the agents by PCR.
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D016477:Artifacts; D002850:Chromatography, Gel / Q000379:methods; D004269:DNA, Bacterial / Q000302:isolation & purification; D004717:Endometrium / Q000382:microbiology; D005260:Female; D006801:Humans; D008598:Menstruation / Q000097:blood; D009169:Mycobacterium tuberculosis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D014384:Tuberculosis, Female Genital / Q000097:blood / Q000175:diagnosis / Q000382:microbiology
D004269:DNA, Bacterial