title: Inhibition of LDL oxidation by melatonin requires supraphysiologic concentrations.
abstract: Melatonin has been suggested as a potent antioxidant that may protect against development of atherosclerosis and cancer; however, these effects are unproven and controversial. The antioxidant capacity of melatonin was tested in comparison with alpha-tocopherol, ascorbic acid, and the melatonin precursors tryptophan and serotonin, by measuring inhibition of metal ion-mediated and human macrophage-mediated oxidation of LDL. Melatonin had weak antioxidant activity that was detectable only at concentrations 10000- to 100000-fold higher than physiologic concentrations. These results were comparable with published data showing that the radical scavenging activity of melatonin requires markedly supraphysiologic concentrations. In contrast, alpha-tocopherol was 50- to 100-fold more potent and was efficacious at physiologic concentrations. Ascorbic acid and tryptophan also were active at physiologic concentrations and were significantly more potent than melatonin. In summary, extremely supraphysiologic concentrations of melatonin had only weak antioxidant activity, which was surpassed by alpha-tocopherol, ascorbic acid, and tryptophan.
authors.affiliation: Division of Endocrinology, Diabetes and Clinical Nutrition, Oregon Health Sciences University, Portland 97201-3098, USA. duellb@ohsu.edu
authors.forename: P B
authors.initials: PB
authors.lastname: Duell
authors.forename: D L
authors.initials: DL
authors.lastname: Wheaton
authors.forename: A
authors.initials: A
authors.lastname: Shultz
authors.forename: H
authors.initials: H
authors.lastname: Nguyen
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism*; D001205:Ascorbic Acid / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D004587:Electrophoresis, Agar Gel; D006801:Humans; D015227:Lipid Peroxidation; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism; D008550:Melatonin / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism; D014364:Tryptophan / Q000737:chemistry / Q000378:metabolism; D014810:Vitamin E / Q000737:chemistry / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D014810:Vitamin E; D012701:Serotonin; D014364:Tryptophan; D008550:Melatonin; D001205:Ascorbic Acid