title: Cloning and sequencing of three C-type lectins from body surface mucus of the land slug, Incilaria fruhstorferi.
abstract: Three C-type lectins of 15 kDa were isolated from the water-soluble fraction (WSF) of the body surface mucus of the land slug, Incilaria fruhstorferi. Based on their partial amino acid sequences, the nucleotide sequences of cDNAs encoding these lectins, named incilarin A, B and C, were determined. cDNAs of incilarin A, B and C consisted of 673, 663 and 715 bp, and deduced amino acids were 150, 149 and 156 residues, respectively. All three lectins had signal peptides of 17 amino acid residues at their N-termini. They showed 44-55% amino acid sequence identity with each other, and lower but significant homology with the other animal C-type lectins and antifreeze protein. Incilarin A and B seem to possess two intramolecular disulfide bonds in the carbohydrate-binding domain (CRD) conserved among the animal C-type lectins, however, one of these bonds is absent in incilarin C.
authors.affiliation: Graduate School of Science, Tohoku University, Sendai, Japan.
authors.forename: H J
authors.initials: HJ
authors.lastname: Yuasa
authors.forename: E
authors.initials: E
authors.lastname: Furuta
authors.forename: A
authors.initials: A
authors.lastname: Nakamura
authors.forename: T
authors.initials: T
authors.lastname: Takagi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D037181:Lectins, C-Type; D008969:Molecular Sequence Data; D008974:Mollusca / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D037181:Lectins, C-Type; D011994:Recombinant Proteins; C114688:incilarin A, Incilaria fruhstorferi; C114689:incilarin B, Incilaria fruhstorferi; C114690:incilarin C, Incilaria fruhstorferi