title: Bradyrhizobium japonicum FixK2, a crucial distributor in the FixLJ-dependent regulatory cascade for control of genes inducible by low oxygen levels.
abstract: Bradyrhizobium japonicum possesses a second fixK-like gene, fixK2, in addition to the previously identified fixK1 gene. The expression of both genes depends in a hierarchical fashion on the low-oxygen-responsive two-component regulatory system FixLJ, whereby FixJ first activates fixK2, whose product then activates fixK1. While the target genes for control by FixK1 are unknown, there is evidence for activation of the fixNOQP, fixGHIS, and rpoN1 genes and some heme biosynthesis and nitrate respiration genes by FixK2. FixK2 also regulates its own structural gene, directly or indirectly, in a negative way.
authors.affiliation: Mikrobiologisches Institut, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
authors.forename: D
authors.initials: D
authors.lastname: Nellen-Anthamatten
authors.forename: P
authors.initials: P
authors.lastname: Rossi
authors.forename: O
authors.initials: O
authors.lastname: Preisig
authors.forename: I
authors.initials: I
authors.lastname: Kullik
authors.forename: M
authors.initials: M
authors.lastname: Babst
authors.forename: H M
authors.initials: HM
authors.lastname: Fischer
authors.forename: H
authors.initials: H
authors.lastname: Hennecke
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial / Q000235:genetics; D006420:Hemeproteins / Q000502:physiology; D000071677:Histidine Kinase; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D009876:Operon / Q000235:genetics; D010100:Oxygen; D011993:Recombinant Fusion Proteins; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D006420:Hemeproteins; D011993:Recombinant Fusion Proteins; C066628:FixK protein, Bacteria; C069289:FixJ protein, Bacteria; C067929:FixL protein, Bacteria; D000071677:Histidine Kinase; D010100:Oxygen