title: Identification of novel genes that are differentially expressed in human colorectal carcinoma.
abstract: By using mRNA differential display technology, we have identified three cDNA clones, myl 3, myl 4, and myl 6, to show significant changes in expression between human colorectal tumor and paired normal tissue. Northern blot analysis indicated that clone myl 3 expression was elevated in normal tissue, and clone myl 4 expression was elevated in tumor tissue. Nucleotide sequence analysis revealed that clones myl 3 and myl 4 have not been previously identified. However, clone myl 6 appears to be the human homolog of the 3' end region of tissue inhibitor of metalloproteinase 3 (TIMP-3). Northern blot analysis showed that a 2.5 kb TIMP-3 transcript was expressed at a much higher level in normal tissue than the colorectal tumor.
authors.affiliation: School of Medical Technology, Chang Gung University, Kweishan, Taoyuan, Taiwan.
authors.forename: E C
authors.initials: EC
authors.lastname: Chan
authors.forename: M Y
authors.initials: MY
authors.lastname: Lue
authors.forename: K C
authors.initials: KC
authors.lastname: Hsu
authors.forename: H A
authors.initials: HA
authors.lastname: Fan
mesh_terms: D000368:Aged; D001483:Base Sequence; D015152:Blotting, Northern; D015179:Colorectal Neoplasms / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D012689:Sequence Homology, Nucleic Acid; D019717:Tissue Inhibitor of Metalloproteinase-3 / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D019717:Tissue Inhibitor of Metalloproteinase-3