Unexpected difference in enantioselective retention on cellulase (CHB I) silica stationary phase caused by exchange of potassium for sodium ion in the mobile phase.
An increase in both retention and enantioselectivity for some beta-blocking agents was observed when exchanging potassium to sodium ion in the buffer used as mobile phase. A large effect of ionic strength on retention was observed, while the enantioselectivity was constant.
Hedeland
M
M
Department of Pharmaceutical Chemistry, Uppsala University, Sweden.
Jönsson
S
S
Isaksson
R
R
Pettersson
C
C
D000319:Adrenergic beta-Antagonists / Q000737:chemistry; D002021:Buffers; D002480:Cellulase / Q000737:chemistry*; D008790:Metoprolol / Q000737:chemistry; D010096:Oxprenolol / Q000737:chemistry; D011188:Potassium / Q000737:chemistry*; D012822:Silicon Dioxide; D012964:Sodium / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism; D013696:Temperature
D000319:Adrenergic beta-Antagonists; D002021:Buffers; D010096:Oxprenolol; D012822:Silicon Dioxide; D012964:Sodium; D002480:Cellulase; D008790:Metoprolol; D011188:Potassium