Glucagon-like peptide-2 inhibits centrally induced antral motility in pigs.
BACKGROUND
Glucagon-like peptide-2 is formed from proglucagon in the intestinal L-cells and is secreted postprandially in parallel with the insulinotropic hormone GLP-1 (glucagon-like peptide-1), which in addition acts to inhibit gastric motility (enterogastrone effect) by inhibiting central parasympathetic outflow. GLP-2 has no effect on the endocrine pancreas. We here tested the hypothesis that GLP-2 acts as an enterogastrone.


METHODS
Fourteen anesthetized pigs with their splanchnic nerves cut were subjected to insulin hypoglycemia, and force transducers were sutured to the antrum to record motility. GLP-2 was infused intravenously in doses from 1 to 6 pmol/kg/min after the onset of antral motility in response to hypoglycemia.


RESULTS
Insulin hypoglycemia invariably and greatly increased the frequency and amplitude of antral phasic contractions. Infusions of GLP-2 dose dependently (1-6 pmol/kg/min) inhibited antral motility. At 2 pmol/kg/min, resulting in plasma GLP-2 concentrations of 102.5+/-19 pmol/l (normal postprandial range, 30-82 pmol/l), the motility index was inhibited by 91%+/-14%.


CONCLUSIONS
Both of the intestinal glucagon-like peptides may operate as hormonal transmitters of the ileal brake effect.
Wøjdemann
M
M
Dept. of Surgical Gastroenterology, Rigshospitalet, The National University Hospital, Copenhagen, Denmark.
Wettergren
A
A
Hartmann
B
B
Holst
J J
JJ
D000818:Animals; D005746:Gastric Emptying; D005768:Gastrointestinal Hormones / Q000008:administration & dosage / Q000502:physiology*; D052216:Glucagon-Like Peptide 1; D053767:Glucagon-Like Peptide 2; D007003:Hypoglycemia / Q000503:physiopathology; D010455:Peptides / Q000008:administration & dosage / Q000502:physiology*; D019518:Postprandial Period; D011706:Pyloric Antrum / Q000502:physiology* / Q000503:physiopathology; D013552:Swine
D005768:Gastrointestinal Hormones; D053767:Glucagon-Like Peptide 2; D010455:Peptides; D052216:Glucagon-Like Peptide 1; C073320:enterogastrone