Effect of pyridoxine and insulin administration on brain glutamate dehydrogenase activity and blood glucose control in streptozotocin-induced diabetic rats.
Blood glucose level and kinetic parameters of glutamate dehydrogenase (GDH) were measured in the cerebellum, brain stem and cerebral cortex of control, insulin treated, pyridoxine treated, pyridoxine and insulin treated and untreated streptozotocin-diabetic rats. The combined administration of insulin and pyridoxine was found to be better in controlling the hyperglycaemia. Insulin with pyridoxine treatment brought back the increased maximal velocity of GDH during diabetes to control state. Also, there was an increase in Michaelis-Menten constant. These results suggest that pyridoxine and insulin together serve a better control for diabetes.
Nair
A R
AR
Molecular Neurobiology and Cell Biology Unit, Department of Biotechnology, Cochin University of Science and Technology, Cochin 682 022, India.
Biju
M P
MP
Paulose
C S
CS
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy / Q000201:enzymology*; D004359:Drug Therapy, Combination; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage / Q000627:therapeutic use*; D007700:Kinetics; D008297:Male; D011736:Pyridoxine / Q000008:administration & dosage / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D007328:Insulin; D005969:Glutamate Dehydrogenase; D011736:Pyridoxine