title: Vitexicarpin, a flavonoid from the fruits of Vitex rotundifolia, inhibits mouse lymphocyte proliferation and growth of cell lines in vitro.
abstract: Certain flavonoids having a C-2,3-double bond were reported to show an inhibitory activity against T-lymphocyte proliferation, but not against B-lymphocyte proliferation in vitro. In the course of these studies, vitexicarpin (3',5-dihydroxy-3,4',6,7-tetramethoxyflavone) isolated from the fruits of Vitex rotundifolia was found to show potent inhibition against lymphocyte proliferation. Vitexicarpin inhibited T-lymphocyte proliferation as well as B-lymphocyte proliferation at > 0.1 microM. IC50's were approximately 0.7 microM both for T- and B-cell proliferation. The inhibitory activity of vitexicarpin was reversible. Vitexicarpin also inhibited the growth of certain cancer cell lines, EL-4 and P815.9 (IC50 = 0.25-0.3 microM). These results suggest that vitexicarpin may be a potential therapeutic agent involved in inflammatory/immunoregulatory disorders such as rheumatoid arthritis and lymphomas.
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon, Korea.
authors.forename: K M
authors.initials: KM
authors.lastname: You
authors.forename: K H
authors.initials: KH
authors.lastname: Son
authors.forename: H W
authors.initials: HW
authors.lastname: Chang
authors.forename: S S
authors.initials: SS
authors.lastname: Kang
authors.forename: H P
authors.initials: HP
authors.lastname: Kim
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000633:toxicity*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020843:Chamomile; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D007739:L Cells; D008213:Lymphocyte Activation / Q000187:drug effects*; D008223:Lymphoma; D008297:Male; D012515:Mast-Cell Sarcoma; D051379:Mice; D008810:Mice, Inbred C57BL; D009822:Oils, Volatile / Q000633:toxicity; D010946:Plants, Medicinal; D011794:Quercetin / Q000633:toxicity; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D005419:Flavonoids; D009822:Oils, Volatile; C054133:casticin; D011794:Quercetin