IL-13 increases the cPLA2 gene and protein expression and the mobilization of arachidonic acid during an inflammatory process in mouse peritoneal macrophages.
Pretreatment of mouse peritoneal macrophages with interleukin-13 (IL-13) potentiates the mobilization of arachidonic acid (AA) and the production of HETEs but does not affect the production of cyclooxygenase metabolites triggered by the suboptimal concentration of an inflammatory agonist (opsonized-zymosan). Cycloheximide suppresses these effects of IL-13 suggesting that de novo protein synthesis is involved. Indeed, IL-13 induces a time-dependent increase in the levels of cytosolic PLA2 (cPLA2) protein and mRNA. This study demonstrates a new pathway for IL-13 to modulate the inflammatory process in macrophages via modifications of cPLA2 expression and subsequent AA mobilization.
Rey
A
A
Laboratoire Macrophages, Médiateurs de l'Inflammation et Interactions Cellulaires, UPS E.A. 2405, Bâtiment L1, Hôpital de Rangueil, Toulouse, France.
M'Rini
C
C
Sozzani
P
P
Lamboeuf
Y
Y
Beraud
M
M
Caput
D
D
Ferrara
P
P
Pipy
B
B
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D005786:Gene Expression Regulation; D006893:Hydroxyeicosatetraenoic Acids / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism*; D018793:Interleukin-13 / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D010741:Phospholipases A / Q000096:biosynthesis / Q000235:genetics*; D054467:Phospholipases A2; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D014316:Tritium; D015054:Zymosan
D001095:Arachidonic Acids; D006893:Hydroxyeicosatetraenoic Acids; D018793:Interleukin-13; D012333:RNA, Messenger; D014316:Tritium; D015054:Zymosan; D003513:Cycloheximide; D010741:Phospholipases A; D054467:Phospholipases A2