PKC1, encoding a protein kinase C, and FAT1, encoding a fatty acid transporter protein, are neighbors in Cochliobolus heterostrophus.
A protein kinase C gene (PKC1) and adjacent DNA of the filamentous ascomycete Cochliobolus heterostrophus was cloned and sequenced. The deduced amino acid sequence of PKC1 shows high homology to PKCs of other filamentous fungi and all define a new subgroup of PKCs. All attempts to disrupt PKC1 failed, suggesting, but not proving, that disruption of PKC1 function is lethal. About 1 kb 3' of PKC1 is FAT1 encoding a putative bifunctional fatty acid transporter/very-long-chain acyl-CoA synthetase.
Oeser
B
B
Institut für Botanik, Westfälische Wilhelms Universität, Münster, Germany. oser@uni-muenster.de
D000595:Amino Acid Sequence; D001203:Ascomycota / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003066:Coenzyme A Ligases / Q000235:genetics; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D011493:Protein Kinase C / Q000145:classification / Q000235:genetics*; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction
D002352:Carrier Proteins; C105342:FAT1 protein, S cerevisiae; D050612:Fatty Acid Transport Proteins; D005227:Fatty Acids; D005656:Fungal Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C097993:Pkc1 protein, Trichoderma reesei; D011493:Protein Kinase C; D003066:Coenzyme A Ligases