title: Expression of trkB mRNA is altered in rat hippocampus after experimental brain trauma.
abstract: Recent investigations have shown that expression of mRNAs for the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) is differentially altered in the hippocampus following traumatic brain injury. In the present study, modulation of neurotrophin receptor expression was examined in the hippocampus in a rat model of traumatic brain injury using in situ hybridization. Messenger RNA for trkB, the high-affinity receptor for BDNF and neurotrophin-4 (NT-4), was increased between 3 and 6 h bilaterally in the dentate gyrus following a lateral fluid-percussion brain injury of moderate severity (2.0-2.1 atm). No time-dependent alterations were observed for trkB mRNA in hippocampal subfields CA1 and CA3. Levels of mRNA for trkC, the high-affinity receptor for NT-3, did not change in any region of the hippocampus. These data demonstrate that lateral fluid-percussion injury modulates expression of trkB mRNA in the hippocampus and support a role for BDNF/trkB signalling mechanisms in secondary events associated with traumatic brain injury.
authors.affiliation: Department of Clinical Sciences, University of Kentucky, Lexington, KY 40536-0003, USA. rrhick00@pop.uky.edu
authors.forename: R R
authors.initials: RR
authors.lastname: Hicks
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H S
authors.initials: HS
authors.lastname: Dhillon
authors.forename: M R
authors.initials: MR
authors.lastname: Prasad
authors.forename: K B
authors.initials: KB
authors.lastname: Seroogy
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000737:chemistry / Q000293:injuries* / Q000378:metabolism*; D004195:Disease Models, Animal; D017403:In Situ Hybridization; D008297:Male; D009473:Neuronal Plasticity / Q000502:physiology; D018696:Neuroprotective Agents / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*
chemical_list: D018696:Neuroprotective Agents; D012333:RNA, Messenger; D020801:Receptor, Ciliary Neurotrophic Factor; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases