[Determination of selected mycotoxins in food. II. Selection of optimal conditions for the determination of fumonisin B1 and B2 in corn products using high performance liquid chromatography].
The aim of this study was to perform a optimised method for determination of fumonisins B1 and B2 in corn products. The manner of extraction and clean-up of corn products extracts as well conditions of reaction of fumonisins with OPA was described. The main steps of optimised analytical procedure were: extraction of sample with methanol and water (3 + 1), clean-up of extracts on SAX column, derivatisation with OPA, and determination by means of RP-HPLC. The mobile phase was a mixture of methanol, water and acetic acid (75 + 24 + 1). Fluorometric detection was made at 370/440 nm. The mean recovery of fumonisins dependent on fortification level and product was 64-95%, limit of detection for each of fumonisins was 15 micrograms/kg.
Czerwiecki
L
L
Zakład Analizy Zywności, Instytut Biotechnologii Przemysłu Rolno-Spozywczego, Warszawa.
D002264:Carboxylic Acids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D005504:Food Analysis / Q000379:methods*; D037341:Fumonisins; D009183:Mycotoxins / Q000032:analysis*; D003313:Zea mays / Q000737:chemistry*
D002264:Carboxylic Acids; D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C056933:fumonisin B1