Isolation of human intestinal defensins from ileal neobladder urine.
We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion.
Porter
E M
EM
Department of Medicine, University of California, Los Angeles 90095, USA.
Poles
M A
MA
Lee
J S
JS
Naitoh
J
J
Bevins
C L
CL
Ganz
T
T
D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000652:urine*; D023082:Defensins; D006801:Humans; D007082:Ileum / Q000378:metabolism* / Q000637:transplantation; D008969:Molecular Sequence Data; D019879:Paneth Cells / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D016476:Urinary Reservoirs, Continent
D001798:Blood Proteins; D023082:Defensins; D010741:Phospholipases A