Molecular cloning and characterization of a plastid chaperonin 60 alpha subunit from Canavalia lineata.
A 2040 bp cDNA encoding plastid chaperonin 60alpha protein was isolated from a cDNA expression library prepared from Canavalia lineata leaves. The sequence analysis of the clone showed a significant homology to those of other plant plastid chaperonin 60alpha. The genomic DNA blot analysis indicated that Canavalia lineata chaperonin 60alpha is encoded by a single gene in the genome. The short-term kinetics of light on the gene expression revealed that the remarkable accumulation of the gene occurred after 24 h. The mRNA was also detectable in dark-grown seedling leaves.
Choi
Y A
YA
Department of Biology, Seoul National University, Korea.
Lee
J S
JS
Kwon
Y M
YM
D000595:Amino Acid Sequence; D001483:Base Sequence; D018834:Chaperonin 60 / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D007887:Fabaceae / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000737:chemistry; D010944:Plants / Q000737:chemistry; D010946:Plants, Medicinal; D018087:Plastids / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018834:Chaperonin 60; D018076:DNA, Complementary; D018744:DNA, Plant; D012333:RNA, Messenger