LIN-10 is a shared component of the polarized protein localization pathways in neurons and epithelia.
We tested the model that neurons and epithelial cells use a shared mechanism for polarized protein sorting by comparing the pathways for localizing basolateral and postsynaptic proteins in C. elegans. GLR-1 glutamate receptors are localized to postsynaptic elements of central synapses and, when ectopically expressed, to basolateral membranes of epithelial cells. Proper localization of GLR-1 in both neurons and epithelia requires the PDZ protein LIN-10, defining LIN-10 as a shared component of the basolateral and postsynaptic localization pathways. Changing the GLR-1 carboxy-terminal sequence from a group I PDZ-binding consensus (-TAV) to a group II consensus (-FYV) restores GLR-1 synaptic localization in lin-10 mutants. Thus, these interneurons utilize at least two separate postsynaptic localization pathways.
Rongo
C
C
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
Whitfield
C W
CW
Rodal
A
A
Kim
S K
SK
Kaplan
J M
JM
D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics; D029742:Caenorhabditis elegans Proteins; D016764:Cell Polarity / Q000502:physiology; D004847:Epithelial Cells / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D015801:Helminth Proteins / Q000235:genetics* / Q000378:metabolism*; D007202:Indicators and Reagents; D007395:Interneurons / Q000737:chemistry / Q000166:cytology* / Q000502:physiology; D008164:Luminescent Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D011506:Proteins; D013569:Synapses / Q000737:chemistry; D013572:Synaptic Vesicles / Q000737:chemistry
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; D007202:Indicators and Reagents; D008164:Luminescent Proteins; D008565:Membrane Proteins; D011506:Proteins; C064105:lin-10 protein, C elegans; D049452:Green Fluorescent Proteins