HMG-17, a chromosomal non-histone protein, shows developmental regulation during organogenesis.
We used the differential hybridization technique for isolating developmentally regulated genes from the mouse metanephric kidney. In this screening, we identified the cDNA encoding high-mobility-group protein 17 (HMG-17), a chromosomal non-histone protein which modulates the conformation of transcriptionally active chromatin. Using Northern blot analysis, the HMG-17 mRNA was strongly expressed during embryogenesis and downregulated in various adult murine organs. At the histological level, the transcript localized to differentiating tissue regions and was apparently downregulated in mature structures indicating that HMG-17 expression is linked to cell differentiation. HMG-17 can thus be regarded as a general marker for tissues or cells undergoing differentiation during organogenesis.
Lehtonen
S
S
Department of Pathology, Haartman Institute, University of Helsinki, Finland.
Olkkonen
V M
VM
Stapleton
M
M
Zerial
M
M
Lehtonen
E
E
D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005819:Genetic Markers; D006609:High Mobility Group Proteins / Q000235:genetics*; D017403:In Situ Hybridization / Q000379:methods; D007668:Kidney / Q000196:embryology*; D008297:Male; D008648:Mesoderm; D051379:Mice; D008808:Mice, Inbred CBA; D009024:Morphogenesis; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis
D018076:DNA, Complementary; D005819:Genetic Markers; D006609:High Mobility Group Proteins; D012333:RNA, Messenger