Development of an integrative DNA transformation system for the yeast Candida utilis.
We report here the development of an auxotrophic transformation system for the food yeast Candida utilis. To facilitate molecular studies in Candida utilis, we isolated auxotrophic strains for uracil biosynthesis by the combination of NTG-mutagenesis and 5-fluorotic acid (FOA) selection. The ura-mutation could be functionally complemented by the homologous URA3 gene. We used both, LiAc and electroporation methods to direct insertions at the ura3 locus through homologous recombination.
Rodríguez
L
L
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Chávez
F P
FP
Basabe
L
L
Rivero
T
T
Delgado
J M
JM
D015139:Blotting, Southern; D002175:Candida / Q000235:genetics* / Q000254:growth & development; D018274:Electroporation; D005656:Fungal Proteins / Q000235:genetics; D005822:Genetic Vectors; D008094:Lithium; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic; D014498:Uracil / Q000096:biosynthesis
D005656:Fungal Proteins; D014498:Uracil; D008094:Lithium