Effects of antioxidants on streptozotocin-induced clastogenesis in mammalian and insect cells.
The effects of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and the hydroxyl radical scavenger mannitol (MAN) on the clastogenesis induced by STZ in Chinese hamster ovary (CHO) and mosquito cells were investigated. The addition of liposome-entrapped SOD, CAT and MAN to both cell lines caused a significant decrease in the yield of STZ-induced chromosome aberrations (p<0.05). However, the inhibitory effect was more evident in CHO (40.6-67.5%) than in mosquito (15.2-53.6%) cells. These findings indicate that the chromosome damage induced by STZ can be partially inhibited through the incorporation of antioxidant compounds into the cells and suggest that free radicals are involved in the clastogenesis by STZ.
Bolzán
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Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900, La Plata, Argentina. imbice@netverk.com.ar
Bianchi
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Bianchi
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D000330:Aedes / Q000166:cytology / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D016466:CHO Cells; D002374:Catalase / Q000494:pharmacology; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D016166:Free Radical Scavengers / Q000494:pharmacology; D017665:Hydroxyl Radical; D008353:Mannitol / Q000494:pharmacology; D009153:Mutagens / Q000633:toxicity*; D013311:Streptozocin / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000494:pharmacology
D000975:Antioxidants; D016166:Free Radical Scavengers; D009153:Mutagens; D017665:Hydroxyl Radical; D008353:Mannitol; D013311:Streptozocin; D002374:Catalase; D013482:Superoxide Dismutase