Correlation between Bcl-X expression and B-cell hybridoma apoptosis induced by activin A.
In this study, we examined the role of bcl-XL and bcl-XS in apoptotic cell death of HS-72 cells induced by activin A. Immunoblot analysis revealed that a band of Bcl-XL was detected in HS-72 cells cultured with or without activin A. Although untreated HS-72 cells did not express Bcl-XS, the expression of Bcl-XS was significantly increased when cultured with activin A. We also investigated the expression of Bcl-XS and Bcl-XL in HS-72 cells cultured with activin A in the presence of protein kinase C inhibitor 1-(5-isoquinotinesulfonyl)-2-methylpiperazine dihydrochloride (H7), which suppressed apoptosis in HS-72 cells induced by activin A. Exposure to H7 apparently increased the level of Bcl-XL in HS-72 cells cultured with or without activin A. In contrast, no detectable band of Bcl-XS was found in HS-72 cells cultured with activin A and H7. These findings indicate that Bcl-XL upregulation and Bcl-XS downregulation induced by H7 might correlate with the suppression of activin A-induced apoptosis in B-lineage cells.
Koseki
T
T
Department of Oral Science, National Institute of Infectious Diseases, Tokyo, Japan.
Yamato
K
K
Ishisaki
A
A
Hashimoto
O
O
Sugino
H
H
Nishihara
T
T
D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine / Q000494:pharmacology; D028341:Activins; D000818:Animals; D017209:Apoptosis; D001402:B-Lymphocytes; D004247:DNA / Q000096:biosynthesis; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006133:Growth Substances / Q000378:metabolism* / Q000494:pharmacology; D006825:Hybridomas; D007265:Inhibins / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis / Q000502:physiology*; D014407:Tumor Cells, Cultured; D051020:bcl-X Protein
C494812:Bcl2l1 protein, mouse; D004791:Enzyme Inhibitors; D006133:Growth Substances; D019253:Proto-Oncogene Proteins c-bcl-2; D051020:bcl-X Protein; D028341:Activins; D007265:Inhibins; D019307:1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; D004247:DNA