Characterisation of high Mr wheat glutenin polymers by agarose gel electrophoresis and dynamic light scattering.
Agarose gel electrophoresis has been used to separate the complex mixture of wheat gluten polymers into fractions ranging in Mr, determined by dynamic light scattering, from about 500,000 to over 5x10(6). The separation is reliable and reproducible and well suited to the routine analysis of multiple samples.
Egorov
T A
TA
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Odintsova
T I
TI
Shewry
P R
PR
Tatham
A S
AS
D004587:Electrophoresis, Agar Gel; D005983:Glutens / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013059:Spectrum Analysis, Raman; D014908:Triticum / Q000737:chemistry*
D010940:Plant Proteins; D005983:Glutens; C037302:glutenin