Regulation of the human glut4 gene expression in tumor RD18 cell line.
We tested if glut4, the gene for muscle-specific glucose transporter, underwent some variations of expression in neoplastic cells. Our model was a rhabdomyosarcoma cell line (RD18) which retains the ability to differentiate along the myogenic pathway. Any definable changes of expression of glut4 in normal and RD18 cells were revealed by Northern blot analysis. In order to identify the transcriptional regulatory regions of the glut4 gene we performed a deletion analysis of the 5' flanking region. The downregulation which we found in the expression of this gene in RD18 cells could be related with the activity of a negative regulatory element.
Chiaramonte
R
R
Department of Biomedical Sciences and Technologies, University of Milan, Italy.
Bartolini
E
E
Testolin
C
C
Comi
P
P
D001483:Base Sequence; D015152:Blotting, Northern; D018211:Chondrosarcoma, Mesenchymal / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D051275:Glucose Transporter Type 4; D006801:Humans; D008969:Molecular Sequence Data; D009004:Monosaccharide Transport Proteins / Q000235:genetics* / Q000378:metabolism; D019042:Muscle Neoplasms / Q000378:metabolism*; D009124:Muscle Proteins; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012208:Rhabdomyosarcoma / Q000378:metabolism; D017421:Sequence Analysis; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured
D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; C495692:SLC2A4 protein, human