Amino-terminal sequences that direct nucleoporin nup153 to the inner surface of the nuclear envelope.
Nup153 is a large O-linked glycoprotein that is a component of the basket-like structure that forms the nucleoplasmic face of nuclear pore complexes (NPCs). The Nup153 molecule has a tripartite structure consisting of N- and C-terminal domains flanking a central zinc finger domain. All of the targeting and assembly information contained within Nup153 is contributed by the N-domain. In fact this region of the molecule can target a cytosolic protein, pyruvate kinase, to the nucleoplasmic face of the NPC. The zinc finger and C-terminal domains appear to have no role in these targeting and assembly activities. Deletion analysis reveals that there are two distinct regions within the Nup153 N-domain that contain different targeting functions. One of these is directly involved in assembly into the NPC while a second overlapping region may target Nup153, as well as other reporter molecules, to the inner face of the nuclear envelope.
Enarson
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Department of Cell Biology and Anatomy, and Cancer Biology Research Group, Faculty of Medicine, The University of Calgary, 3330 Hospital Drive NW, Calgary AB, Canada T2N 4N1.
Enarson
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Bastos
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Burke
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B
D019943:Amino Acid Substitution; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D006224:Cricetinae; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D008647:Mesocricetus; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D009685:Nuclear Envelope / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection
C079377:NUP153 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D011993:Recombinant Fusion Proteins