title: Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity.
abstract: Expression and stability of immunoglobulins in transgenic plants have been investigated and optimized by accumulation in different cellular compartments as cytosol, apoplastic space and endoplasmic reticulum (ER) as will be discussed in this review. In several cases described the highest accumulation of complete active antibodies was achieved by targeting into the apoplastic space. High-level expression of active recombinant single-chain Fv antibodies (scFv's) was obtained by retention of these proteins in the lumen of the endoplasmic reticulum. This has been shown for leaves and seeds of transgenic tobacco as well as for potato tubers. Transgenic tobacco seeds, potato tubers and tobacco leaves can facilitate stable storage of scFv's accumulated in the ER over an extended (seeds, tubers) or a short (leaves) period of time. The expression of specific scFv's in different plant species, plant organs and cellular compartments offers the possibility of blocking regulatory factors or pathogens specifically. Examples are scFv's expressed in the cytosol and the apoplastic space of transgenic plant cells modulating the infection process of plant viruses and a cytosolically expressed scFv that influenced the activity of phytochrome A protein. The immunomodulation approach has been shown to be also applicable for investigating the action of the phyto-hormone abscisic acid (ABA). High-level accumulation of specific anti-ABA scFv's in the ER of all leaf cells has been used to block the influence of ABA on the stomatal functions. Seed-specific expression of high amounts of anti-ABA-scFv's at a defined time of seed-development induced a developmental switch from seed ripening to vegetative growth. It has been demonstrated that ER retention is essential for the accumulation of sufficient scFv to bind high concentrations of ABA in the transgenic seeds.
authors.affiliation: Institut für Pflanzengenetik und Kulturpflanzenforschung Gatersleben, Germany. conradu@IPK-gatersleben.de
authors.forename: U
authors.initials: U
authors.lastname: Conrad
authors.forename: U
authors.initials: U
authors.lastname: Fiedler
mesh_terms: D000276:Adjuvants, Immunologic / Q000502:physiology*; D000818:Animals; D002451:Cell Compartmentation / Q000276:immunology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D030821:Plants, Genetically Modified / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism*
chemical_list: D000276:Adjuvants, Immunologic; D007136:Immunoglobulins; D011994:Recombinant Proteins