Specificity of bactericidal antibody response to serogroup B meningococcal strains in Brazilian children after immunization with an outer membrane vaccine.
Pre- and postvaccination serum samples from 77 children aged 2 to 6 years, who received the Cuban BC vaccine (B:4:P1.15), were analyzed for bactericidal antibodies against a local B:4:P1.15 strain (N44/89). Sera from 16 individuals with bactericidal antibodies against the B:4:P1.15 strain were tested against 23 Brazilian isolates. These include B:4 strains of distinct serosubtypes: P1.15, P1.7,1, P1.3, P1.9, P1.nt, and a B:8,19,23:P1.16 strain. A Cuban B:4:P1.15 strain (Cu385/83) was also included in the study. The specificities of bactericidal antibodies were analyzed by using mutant strains lacking a class 1 protein (PorA protein) or a class 5 protein or both. The results indicated that PorA and class 5 proteins are the main targets recognized by the bactericidal antibodies of vaccinees. Nonetheless, a complex pattern of recognition by bactericidal antibodies was found, and vaccinees were grouped according to antibody specificity. Antibodies from some individuals recognized PorA of serosubtype P1.15. However, antibodies from these individuals could not kill all P1.15 strains tested. Antibodies from a second group recognized both PorA and class 5 proteins, and antibodies from a third group recognized an as yet unidentified target antigen. The results demonstrate the importance of determining the fine epitope specificity of bactericidal antibodies to improve the existing vaccines against B meningococci.
Milagres
L G
LG
Bacteriology Division, Adolfo Lutz Institute, São Paulo, SP 01246-902, Brazil.
Gorla
M C
MC
Sacchi
C T
CT
Rodrigues
M M
MM
D000907:Antibodies, Bacterial / Q000097:blood*; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins / Q000627:therapeutic use*; D001428:Bacterial Vaccines / Q000627:therapeutic use*; D001938:Brazil; D002648:Child; D002675:Child, Preschool; D006801:Humans; D008589:Meningococcal Infections / Q000276:immunology / Q000517:prevention & control*; D022401:Meningococcal Vaccines; D008969:Molecular Sequence Data; D009154:Mutation; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics; D018272:Porins / Q000235:genetics; D012703:Serotyping; D014611:Vaccination
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; C114926:Cuban OMP vaccine; D022401:Meningococcal Vaccines; D018272:Porins; C057699:porin protein, Neisseria