title: Lymphocyte triggering via L-selectin leads to enhanced galectin-3-mediated binding to dendritic cells.
abstract: For proper immune surveillance, naive lymphocytes are recruited from the blood into secondary lymphoid organs. L-selectin expressed on lymphocytes plays an important role in the initial attachment of these cells to high endothelial venules (HEV) in lymph nodes. Previously, we found that triggering via L-selectin resulted in activation of lymphocytes, followed by an alteration in their adhesion capacity. This suggested that L-selectin triggering might play a role in cell-cell interactions after lymph node entry. Here, we identify a novel adhesion mechanism involving L-selectin-triggered lymphocytes and dendritic cells, and we show that enhanced binding to dendritic cells is mediated by galectin-3 and not by integrins. Furthermore, it was shown that L-selectin-triggered T lymphocytes exhibited enhanced proliferation in an allogeneic mixed lymphocyte reaction. It is concluded that, in addition to a role for L-selectin in tethering and rolling on endothelium, triggering of the molecule on the lymphocyte surface leads to changes that are pertinent for the function of the cell after passing the HEV. We argue that the described adhesion mechanism plays a role in optimizing the initial interaction between dendritic cells and lymphocytes.
authors.affiliation: Department of Medical Chemistry, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: V V
authors.initials: VV
authors.lastname: Swarte
authors.forename: R E
authors.initials: RE
authors.lastname: Mebius
authors.forename: D H
authors.initials: DH
authors.lastname: Joziasse
authors.forename: D H
authors.initials: DH
authors.lastname: Van den Eijnden
authors.forename: G
authors.initials: G
authors.lastname: Kraal
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000276:immunology; D002241:Carbohydrates / Q000494:pharmacology; D002450:Cell Communication / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D037502:Galectin 3; D019041:L-Selectin / Q000276:immunology*; D037102:Lectins; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D000943:Antigens, Differentiation; D002241:Carbohydrates; D037502:Galectin 3; D037102:Lectins; D019041:L-Selectin