Identification of N- and O-linked oligosaccharides in the human epididymis.
The oligosaccharide sequences of glycoconjugates in the human normal epididymis and the nature of linkages were studied with lectin histochemistry. The usual terminal sequences of oligosaccharide side chains in epithelial cell secretions were Neu5Ac2,3Galbeta1,3GalNAc; SO4Galbeta1,3GalNAc; and Galbeta1,4GlcNAc, and they were mainly found in O-linked glycoproteins. The lectin pattern of mitochondria-rich cells differed from that of principal cells.
Arenas
M I
MI
Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid.
Madrid
J F
JF
Bethencourt
F R
FR
Fraile
B
B
Paniagua
R
R
D000328:Adult; D000368:Aged; D004822:Epididymis / Q000737:chemistry* / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D037102:Lectins / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D009844:Oligosaccharides / Q000032:analysis*
D037102:Lectins; D009844:Oligosaccharides