MKP-1 induced in rat brain after electroconvulsive shock is independent of regulation of 42- and 44-kDa MAPK activity.
Electroconvulsive shock (ECS) activates MAPKs in rat brain and also induces immediate early genes. We investigated whether ECS induces MKP-1, a specific MAPK phosphatase and an immediate early gene, for feedback regulation of MAPK activity. ECS induced MKP-1 in the cortex, but MAPK activity returned to its basal level before MKP-1 protein increased, within 10 min of ECS. MKP-1 protein amount peaked 1 hr after ECS. MKP-1 induced did not lower the basal level of MAPK activity or attenuate MAPK activation by second ECS. MAPK activation in cerebellum was very weak, but the MKP-1 induction was faster and more prominent than in the cortex. These results suggest that ECS induces MKP-1 in various rat brain regions, however, the induction may not be related to the activation of MAPK and the MKP-1 induced may be independent of the regulation of MAPK activity after ECS.
Jeon
S H
SH
Department of Biochemistry, Seoul National University College of Medicine, Korea.
Yoo
B H
BH
Kang
U K
UK
Ahn
Y M
YM
Bae
C D
CD
Park
J B
JB
Kim
Y S
YS
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D054638:Dual Specificity Phosphatase 1; D004597:Electroshock; D004789:Enzyme Activation; D005246:Feedback; D017874:Immediate-Early Proteins / Q000096:biosynthesis*; D008297:Male; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley
D018797:Cell Cycle Proteins; D017874:Immediate-Early Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1; D054638:Dual Specificity Phosphatase 1; C517021:Dusp1 protein, rat; D017027:Protein Tyrosine Phosphatases