The effects of nitric oxide synthase inhibitors on acute necrotising pancreatitis in rats.
OBJECTIVE
To investigate the effects of the constitutive nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-Name) and the inducible NO synthase inhibitor amino ethyl-isothiourea (AE-ITU) on acute necrotising pancreatitis (ANP) in rats.


METHODS
Laboratory study.


METHODS
Medical school, Turkey.


METHODS
Morbidity, mortality, effects on activities of various enzymes, and histological picture.


RESULTS
NO inhibitors increased the mortality (from 8/15, 53%, for ANP plus saline, to 12/15, 80%, for ANP plus L-Name, and 13/15, 87%, for ANP plus AE-ITU and serum amylase activity, but had no effects on serum calcium concentrations, volume of ascites, or degree of pancreatic damage. L-Name caused hypoglycemia, and AE-ITU reduced activities of lactate dehydrogenase and liver transaminases, and concentrations of urea and creatinine.


CONCLUSIONS
Constitutive NO synthase inhibition worsens the course of ANP, and inducible NO inhibition has beneficial effects on various systems.
Alhan
E
E
Department of Surgery, Farabi Hospital, Medical Faculty, Karadeniz Technical University, Trabzon, Turkey.
Küçütülü
U
U
Erçin
C
C
D000681:Amylases / Q000097:blood; D000818:Animals; D002118:Calcium / Q000097:blood; D003404:Creatinine / Q000097:blood; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007550:Isothiuronium / Q000031:analogs & derivatives* / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000201:enzymology; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052247:Nitric Oxide Synthase Type II; D010179:Pancreas / Q000473:pathology; D019283:Pancreatitis, Acute Necrotizing / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D000637:Transaminases / Q000378:metabolism; D014508:Urea / Q000097:blood
D004791:Enzyme Inhibitors; D007550:Isothiuronium; C007687:etiron; D014508:Urea; D003404:Creatinine; D007770:L-Lactate Dehydrogenase; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; D000637:Transaminases; D000681:Amylases; D002118:Calcium; D019331:NG-Nitroarginine Methyl Ester