Idiopathic dilated cardiomyopathy: a superantigen-driven autoimmune disease.
BACKGROUND
Many cases of idiopathic dilated cardiomyopathy (IDC) result from an inflammatory myocarditis. The specific immunological mechanisms are not yet defined. Various autoimmune diseases are associated with superantigen-triggered immune responses, resulting in massive T-cell activation and tissue damage. We studied 3 cases in a search for evidence that such a phenomenon is also implicated in IDC.


RESULTS
Myocardial, lymph node, and thymic tissue samples were obtained from IDC patients who were undergoing heart transplantation. Infiltrating immune-cell phenotypes and gene expression of T-cell receptor (TCR) alpha- and beta-chain variable (Valpha and Vbeta) regions were analyzed by immunostaining and polymerase chain reaction. Similar technical approaches were used to assay the tissues for the presence of coxsackievirus B (CVB). In all the specimens analyzed, an overexpression of the TCR Vbeta3, Vbeta7, and Vbeta13.1 gene families was detected among the infiltrating T cells. These tissues were also found to be CVB3-positive. In vitro exposure of peripheral blood mononuclear cells to lysates of cells infected with CVB3 was capable of stimulating expansion of the same TCR Vbeta families. The TCR Valpha repertoire was never found to be skewed.


CONCLUSIONS
A superantigen-mediated immune response is involved in human heart disease. CVB3 may directly or indirectly trigger this response, suggesting a possible mechanistic link between CVB infection and myocarditis development progressing to IDC.
Luppi
P
P
Department of Pediatrics, University of Pittsburgh School of Medicine and Children's Hospital, PA, USA.
Rudert
W A
WA
Zanone
M M
MM
Stassi
G
G
Trucco
G
G
Finegold
D
D
Boyle
G J
GJ
Del Nido
P
P
McGowan
F X
FX
Trucco
M
M
D000818:Animals; D000917:Antibody Formation; D001327:Autoimmune Diseases / Q000097:blood*; D002311:Cardiomyopathy, Dilated / Q000209:etiology* / Q000276:immunology; D002675:Child, Preschool; D002522:Chlorocebus aethiops; D005260:Female; D015894:Genome, Human; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008297:Male; D009206:Myocardium / Q000276:immunology; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D018089:Superantigens / Q000097:blood*; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014709:Vero Cells
D011948:Receptors, Antigen, T-Cell; D018089:Superantigens