Hydrogen peroxide in exhaled air of healthy children: reference values.
An increased content of hydrogen peroxide (H2O2), a marker of inflammation, has been described in the condensate of exhaled air from adults and children with inflammatory lung disorders, including asthma. However, the normal range of [H2O2] in the exhaled air condensate from healthy children has not been established. Therefore, the aim of this study was to determine the reference range of exhaled [H2O2] in healthy school-aged children. Ninety-three healthy nonsmoking children (48 female and 45 male, mean age 10 yrs, range 8-13 yrs), with a negative history for allergy, eczema or respiratory disease and with a normal lung function, participated. Exhaled air condensate was examined fluorimetrically for the presence of H2O2. In addition, the reproducibility of [H2O2] within subjects and between days and the stability of [H2O2] during storage at -20 degrees C were assessed. The median [H2O2] in the exhaled air condensate of all children was 0.13 microM, with a 2.5-97.5% reference range of <0.01-0.48 microM. No significant difference existed between males and females. There was no correlation between exhaled [H2O2] and age or lung function. Repeated [H2O2] measurements on 2 consecutive days showed satisfactory within-subject reproducibility and [H2O2] in stored samples remained stable for at least 1 month at -20 degrees C. In conclusion, this study provides reference data for exhaled hydrogen peroxide in a large group of healthy children. The observed levels were lower than those reported previously for healthy adults and were independent of age, sex and lung function.
Jöbsis
Q
Q
Dept of Paediatrics, Erasmus University and University Hospital/Sophia Children's Hospital, Rotterdam, The Netherlands.
Raatgeep
H C
HC
Schellekens
S L
SL
Hop
W C
WC
Hermans
P W
PW
de Jongste
J C
JC
D001944:Breath Tests; D002648:Child; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis*; D008297:Male; D012016:Reference Values; D015203:Reproducibility of Results; D012129:Respiratory Function Tests
D006861:Hydrogen Peroxide