Inhibitory effects of Angelica pubescens f. biserrata on 5-lipoxygenase and cyclooxygenase.
Linoleic acid, osthol, osthenol and two polyacetylenes, falcarindiol and 11(S),16(R)-dihydroxyoctadeca-9Z,17-diene-12,14-diyn-1 -yl acetate were found to be the most active compounds responsible for the inhibitory activity of the dichloromethane extract of the roots of Angelica pubescens f. biserrata on 5-lipoxygenase (5-LO) and cyclooxygenase (COX-1) in vitro. They showed prominent inhibitory effect on 5-LO with IC50 values of 27.9 microM, 36.2 microM, 43.1 microM, 9.4 microM and 24.0 microM, respectively. Linoleic acid, osthenol, falcarindiol and 11(S), 16(R)-dihydroxyoctadeca-9Z,17-diene-12,14-diyn-1-yl acetate exhibited inhibitory activity on COX-1 with IC50 values of 13.3 microM, 64.3 microM, 66.0 microM and 73.3 microM.
Liu
J H
JH
Institute of Pharmaceutical Biology, University Düsseldorf, Germany.
Zschocke
S
S
Reininger
E
E
Bauer
R
R
D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000097:blood; D003374:Coumarins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051545:Cyclooxygenase 1; D016861:Cyclooxygenase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D053280:Diynes; D005233:Fatty Alcohols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D007527:Isoenzymes / Q000378:metabolism*; D007962:Leukocytes / Q000201:enzymology; D019787:Linoleic Acid / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors; D008297:Male; D008516:Medicine, Chinese Traditional; D008861:Microsomes / Q000201:enzymology; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018517:Plant Roots; D010946:Plants, Medicinal; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep; D013552:Swine
D003374:Coumarins; D016861:Cyclooxygenase Inhibitors; D053280:Diynes; D005233:Fatty Alcohols; D007527:Isoenzymes; D016859:Lipoxygenase Inhibitors; D010936:Plant Extracts; C114745:osthenol; C034379:falcarindiol; D019787:Linoleic Acid; D001094:Arachidonate 5-Lipoxygenase; D051545:Cyclooxygenase 1; D011451:Prostaglandin-Endoperoxide Synthases