title: Peptide substrates dissolved in dimethylformamide may be modified at the epsilon-amino group of lysyl residues, causing erroneous kinetic characterization of proteolytic enzymes.
authors.affiliation: Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark. kol@crc.dk
authors.forename: K
authors.initials: K
authors.lastname: Olesen
authors.forename: K
authors.initials: K
authors.lastname: Breddam
mesh_terms: D002268:Carboxypeptidases / Q000378:metabolism; D043402:Cathepsin A; D004126:Dimethylformamide / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D010455:Peptides; D004126:Dimethylformamide; D002268:Carboxypeptidases; D010450:Endopeptidases; D043402:Cathepsin A; D008239:Lysine