title: MaMi, a human endometrial stromal sarcoma cell line that constitutively produces interleukin-6, interleukin-8, and monocyte chemoattractant protein-1.
abstract: BACKGROUND
  Uterine endometrial stromal sarcoma is a rare neoplasm with a morphology that closely resembles that of the proliferative endometrial stroma. To understand its pathologic characteristics, we established a novel cell line, MaMi, from a primary culture of an endometrial stromal sarcoma obtained from a 65-year-old Japanese woman.
  
  
  METHODS
  We observed the morphology of MaMi cells and performed immunohistochemical analysis on the primary tumor and transplants in nude mice. Prolactin, insulin-like growth factor-binding protein-1, interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, E-selectin, vascular cell adhesion molecule-1, and fibronectin production in the culture medium of MaMi cells were also examined.
  
  
  RESULTS
  MaMi cells were shown to exhibit a fibroblast-like morphology in vitro, and they adopted a more elongated appearance in response to 12-O-tetradecanoyl phorbol-13-acetate (TPA). On injection into nude mice, the cells gave rise to subcutaneous tumors. Immunohistologically, both the primary tumor and MaMi cell-induced tumors stained positively with antibodies to neuron-specific enolase or vimentin. MaMi cells constitutively produced IL-6, IL-8, and monocyte chemoattractant protein-1 in vitro. Interleukin-1beta, (100 pmol/L), tumor necrosis factor-alpha (1 nmol/L), and lipopolysaccharide (1 microg/mL) each increased the release of IL-6, IL-8, and monocyte chemoattractant protein-1 by MaMi cells. TPA (10 nmol/L) also stimulated the production of IL-6 and IL-8 by these cells, but inhibited that of monocyte chemoattractant protein-1.
  
  
  CONCLUSIONS
  We demonstrated that MaMi cells closely resemble proliferative endometrial stromal cells not only morphologically, but also functionally. This cell line may prove valuable in understanding the role of cytokines produced by tumor cells in the pathogenesis of endometrial stromal sarcoma and may also be useful as an in vitro model of functioning endometrial stromal cells.
authors.affiliation: Department of Obstetrics and Gynecology, Oita Medical University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Nasu
authors.forename: N
authors.initials: N
authors.lastname: Matsui
authors.forename: H
authors.initials: H
authors.lastname: Narahara
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Takai
authors.forename: I
authors.initials: I
authors.lastname: Miyakawa
authors.forename: Y
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D000368:Aged; D000818:Animals; D002455:Cell Division; D018932:Chemokine CCL2 / Q000032:analysis / Q000096:biosynthesis*; D019040:E-Selectin / Q000032:analysis; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D005353:Fibronectins / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000032:analysis / Q000096:biosynthesis*; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011388:Prolactin / Q000032:analysis; D012509:Sarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D012513:Sarcoma, Experimental / Q000737:chemistry / Q000473:pathology; D017154:Stromal Cells / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D014407:Tumor Cells, Cultured; D019010:Vascular Cell Adhesion Molecule-1 / Q000032:analysis
chemical_list: D018932:Chemokine CCL2; D019040:E-Selectin; D005353:Fibronectins; D018970:Insulin-Like Growth Factor Binding Protein 1; D015850:Interleukin-6; D016209:Interleukin-8; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D011388:Prolactin