pH-sensitive liposomes for receptor-mediated delivery to chicken hepatoma (LMH) cells.
pH-sensitive liposomes composed of dioleoylphosphatidylethanolamine and cholesterol hemisuccinate (3:2 mol/mol) bearing the N-acetylglucosamine derivative of bovine serum albumin (N-Ac-BSA) were applied for receptor-mediated delivery in chicken hepatoma (LMH) cells expressing the N-Ac-BSA-binding asialoglycoprotein receptor. Fluorescently labeled dextran was entrapped in liposomes by a modified freeze-thawing method (encapsulation efficiency of 23%). A novel method of coupling proteins onto the surface of preformed liposomes yielded a coupling efficiency of 60-70%. The association of pH-sensitive and lecithin liposomes with LMH cells was monitored by fluorescence-activated cell sorting and confocal microscopy. Prerequisites for receptor-mediated delivery to LMH cells were both the pH sensitivity of liposomes and the presence of N-Ac-BSA on the liposomal surface.
Skalko
N
N
Department of Pharmaceutical Technology, Pharmaceutical Institute, University of Freiburg, Germany.
Peschka
R
R
Altenschmidt
U
U
Lung
A
A
Schubert
R
R
D000818:Animals; D037263:Asialoglycoprotein Receptor; D002469:Cell Separation; D002645:Chickens; D003911:Dextrans; D005434:Flow Cytometry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives; D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D008114:Liver Neoplasms, Experimental / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D011956:Receptors, Cell Surface / Q000378:metabolism*
D037263:Asialoglycoprotein Receptor; D003911:Dextrans; D008081:Liposomes; D011956:Receptors, Cell Surface; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate