title: Detection of trisomy 3 in primary gastric B-cell lymphoma by using chromosome in situ hybridization on paraffin sections.
abstract: Recent studies in Western populations have shown that trisomy 3 is the most frequent chromosomal abnormality in primary gastric lymphoma (PGL). To study the incidence of trisomy 3 and its implications for the pathogenesis of PGL in Hong Kong, we have applied the technique of chromosome in situ hybridization in 13 cases of PGL by using archival paraffin-embedded tissue sections. Five cases of chronic gastritis were used as controls. Trisomy 3 was found in 9 (69%) of 13 cases, including cases of low-grade lymphoma and high-grade lymphoma with or without a low-grade component. Our findings are similar to the incidence of trisomy 3 reported in the Western literature. The persistent finding of trisomy 3 in various histologic grades of PGL suggests that it may be useful as a clonal marker in this group of neoplasms. Various molecular events involving chromosome 3 may be related to the pathogenesis of this group of lymphomas.
authors.affiliation: Department of Pathology, the University of Hong Kong, Hong Kong.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: A N
authors.initials: AN
authors.lastname: Cheung
authors.forename: A C
authors.initials: AC
authors.lastname: Chan
authors.forename: D H
authors.initials: DH
authors.lastname: Shen
authors.forename: W S
authors.initials: WS
authors.lastname: Xu
authors.forename: L P
authors.initials: LP
authors.lastname: Chung
authors.forename: F C
authors.initials: FC
authors.lastname: Ho
mesh_terms: D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D005756:Gastritis / Q000235:genetics / Q000473:pathology; D006723:Hong Kong; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization / Q000379:methods; D016393:Lymphoma, B-Cell / Q000235:genetics* / Q000473:pathology; D012189:Retrospective Studies; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014314:Trisomy