Normal myoblast implantation in MDX mice prevents muscle damage by exercise.
One consequence of the lack of dystrophin is a higher vulnerability of myofibers to eccentric exercise. In this study, we compared the effect of downhill running on Biceps brachii of MDX mice with or without transplantation of normal myoblasts. Exercise induced damaged was detected by Evans blue staining. In control MDX mice, 26.3% of the fibers were permeated by this dye, myoblast transplantation prevented such necrosis. In the transplanted muscles, only dystrophin negative fibers were injured. Indeed, in muscles containing at least 40% dystrophin positive fibers, the damage was significantly reduced in the grafted muscle. Thus the transplantation of normal myoblasts increases the resistance of dystrophic muscles to exercise. Our results suggest that transplantation of normal myoblasts to DMD patients may have beneficial effects.
Brussee
V
V
Unité de recherche en Génétique Humaine, CHUL, Ste Foy, Canada.
Merly
F
F
Tardif
F
F
Tremblay
J P
JP
D000818:Animals; D017690:Cell Transplantation; D016189:Dystrophin / Q000502:physiology*; D051379:Mice; D018101:Mice, Inbred mdx; D018482:Muscle, Skeletal / Q000473:pathology* / Q000637:transplantation*; D009137:Muscular Dystrophy, Animal / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D010805:Physical Conditioning, Animal
D016189:Dystrophin