Coagglutination test: a simple and rapid immunodiagnostic test for parvovirus infection in dogs.
The coagglutination test (COAT) was developed and standardized to detect canine parvovirus (CPV) antigen in faeces of infected dogs. Anti-parvovirus serum was raised in dogs for coating protein-A containing Staphylococcus aureus Cowan I strain. Agglutination of antibody coated bacteria invariably occurred within 2-3 min when mixed with standard CPV antigen or faecal supernatants of dogs having 8 or more haemagglutination (HA) titre of parvovirus antigen. The test had a perfect correlation with HA test and was found to be slightly more sensitive than agar gel precipitation test (AGPT) in detecting CPV antigens. As COAT is easy and needs no specific equipment or much technical know how to perform, it can be used as a field test for rapid clinical diagnosis of parvovirus infection in dogs.
Singh
B R
BR
College of Veterinary Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, India.
Yadav
R C
RC
Singh
S P
SP
Sharma
V D
VD
D000372:Agglutination Tests / Q000379:methods* / Q000592:standards; D000818:Animals; D004283:Dog Diseases / Q000175:diagnosis* / Q000821:virology; D004285:Dogs; D010322:Parvoviridae Infections / Q000175:diagnosis* / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000276:immunology / Q000302:isolation & purification; D012680:Sensitivity and Specificity