A RAPD-PCR method for large-scale typing of Bacillus cereus.
A robust RAPD-PCR procedure for large-scale typing of Bacillus cereus was developed. It is based on a simple DNA preparation, involving only freezing and boiling of cells in water with active carbon. By using a computerized system for data collection and processing, an efficient system for handling RAPD patterns for large-scale investigations was achieved. The procedure was highly discriminatory for Bacillus cereus strains and was found to give reproducible classification of RAPD fingerprints for five reference strains.
Nilsson
J
J
Swedish Dairies' Association, Research and Development Department, Lund, Sweden.
Svensson
B
B
Ekelund
K
K
Christiansson
A
A
D000818:Animals; D001409:Bacillus cereus / Q000145:classification* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003612:Dairying; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results
D004269:DNA, Bacterial