[Evaluation of indirect immunofluorescence as a supplementary test for the diagnosis of HIV-1 infection].
In order to be used as an alternative or complementary test to confirm HIV-1 infection, the efficiency of indirect immunofluorescence assay (IFA) was compared with Western blot (WB) in 362 samples from persons with high and low risk behaviour. A panel of sera with 220 WB positive, 122 WB negative and 20 WB indeterminate sera were tested by an "in house" IFA. The sensitivity of IFA was found to be 98.63% and the specificity 98.36%. Therefore, IFA appeared to be an efficient alternative method to WB, since the cost of testing by IFA is less than 10% of WB testing. We observed a direct relationship between WB protein reactivity and IFA results. In 15 samples with coincident indeterminate results for WB and IFA, antibody reactivity to p24 and gp160 presented the highest frequency. On the other hand, antibodies to viral glycoproteins were always present in IFA weak positive samples, showing their high predictive value.
Ceballos
A
A
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
Devito
C
C
Pampuro
S
S
Gómez Carrillo
M
M
Libonatti
O
O
Martínez Peralta
L
L
D015492:AIDS Serodiagnosis / Q000379:methods*; D000328:Adult; D001782:Blood Donors; D015153:Blotting, Western; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D015483:HIV Antibodies / Q000032:analysis*; D015488:HIV Antigens / Q000276:immunology; D015658:HIV Infections / Q000175:diagnosis*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008297:Male; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis; D011933:Reagent Kits, Diagnostic; D012309:Risk-Taking; D012680:Sensitivity and Specificity
D015483:HIV Antibodies; D015488:HIV Antigens; D011933:Reagent Kits, Diagnostic