title: Differential transferrin receptor density in human colorectal cancer: A potential probe for diagnosis and therapy.
abstract: Transferrin receptor density was investigated in human colorectal surgical specimens. Crude membranes were prepared from 23 cancer tumors (adenocarcinoma or malignant villous tumor) and 3 non-cancer tumors (polyadenoma or villous tumor) and 26 adjacent control mucosa. Contrary to non-cancer tumors, Scatchard analysis of 125I-transferrin binding data evidenced higher maximal transferrin binding capacity and lower dissociation constant in cancer tissues (Bmax cancer 1.828+/-0.320 nmol/g, Kd 24.1+/-4.7 nM), as compared to paired control colonic mucosa (Bmax contol 0.851+/-0.182 nmol/g, Kd 30.7+/-7.3 nM), paired t-tests: Bmax p<0.001, Kd p<0.05). As the cancer/control Bmax ratio was 2.6+/-0.4,transferrin carrier constructs should be proposed for cancer imaging or therapy.
authors.affiliation: Service Central de Medecine Nucleaire, Centre Hospitalier Le Mans, 72037 Le-Mans, France.
authors.forename: A C
authors.initials: AC
authors.lastname: Prost
authors.forename: F
authors.initials: F
authors.lastname: Ménégaux
authors.forename: P
authors.initials: P
authors.lastname: Langlois
authors.forename: J M
authors.initials: JM
authors.lastname: Vidal
authors.forename: M
authors.initials: M
authors.lastname: Koulibaly
authors.forename: J L
authors.initials: JL
authors.lastname: Jost
authors.forename: J J
authors.initials: JJ
authors.lastname: Duron
authors.forename: J P
authors.initials: JP
authors.lastname: Chigot
authors.forename: P
authors.initials: P
authors.lastname: Vayre
authors.forename: A
authors.initials: A
authors.lastname: Aurengo
authors.forename: J C
authors.initials: JC
authors.lastname: Legrand
authors.forename: G
authors.initials: G
authors.lastname: Rosselin
authors.forename: C
authors.initials: C
authors.lastname: Gespach
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D011869:Radioligand Assay; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism*; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism
chemical_list: D015415:Biomarkers; D011990:Receptors, Transferrin; D014168:Transferrin