title: Mitosis in wild-type and beta-tubulin mutant strains of Aspergillus nidulans.
abstract: We review and illustrate the wild-type mitotic cycle of Aspergillus nidulans and report the sequence alterations in six mutant alleles of the A. nidulans benA, beta-tubulin, gene. These alleles confer heat sensitivity and resistance to the antifungal, antimicrotubule compound benomyl, and they have been very important in the study of mitosis and microtubule function in A. nidulans. The mutations are novel and fall at amino acids 50, 134, and 257. We have examined the phenotypes conferred by the mutations at restrictive temperatures. None blocks the assembly of microtubules. One allele, benA33, blocks anaphase A and partially inhibits the disassembly of cytoplasmic microtubules in mitosis. We also often observe abnormal spindle morphologies in strains carrying benA33. Another allele, benA31, causes arrest in mitosis with short mitotic spindles and, thus, appears to inhibit spindle elongation.
authors.affiliation: Department of Molecular Genetics, The Ohio State University, 484 West 12th Avenue, Columbus, Ohio, 43210, USA. jung4@osu.edu
authors.forename: M K
authors.initials: MK
authors.lastname: Jung
authors.forename: G S
authors.initials: GS
authors.lastname: May
authors.forename: B R
authors.initials: BR
authors.lastname: Oakley
mesh_terms: D000483:Alleles; D001233:Aspergillus nidulans / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D001542:Benomyl / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005659:Fungicides, Industrial / Q000494:pharmacology; D005800:Genes, Fungal / Q000187:drug effects; D006358:Hot Temperature; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure; D008938:Mitosis; D009154:Mutation; D010641:Phenotype; D012680:Sensitivity and Specificity; D014404:Tubulin / Q000235:genetics*
chemical_list: D005659:Fungicides, Industrial; D014404:Tubulin; D001542:Benomyl