Signalling through the leukotriene B4 receptor involves both alphai and alpha16, but not alphaq or alpha11 G-protein subunits.
COS-7 cells transfected with the leukotriene (LT) B4 receptor (BLTR) cDNA were unable to produce LTB4-induced inositol phosphates (IPs) in spite of the presence of endogenous Galphai, Galphaq and Galpha11 proteins. Co-transfection of BLTR with Galpha16, however, resulted in high levels of IP production, which were 17-, 10- and 6-fold higher than with co-transfected Galpha11, Galphaq and Galpha14, respectively. Co-transfection of BLTR with phospholipase C (PLC) beta2, on the other hand, resulted in efficient IP production and co-transfection of BLTR with both Galpha16 and PLCbeta2 resulted in a greater than additive response.
Gaudreau
R
R
Immunology Division, Department of Pediatrics, Faculty of Medicine, Université de Sherbrooke, Sherbrooke, QC J1H 5N4 Canada.
Le Gouill
C
C
Métaoui
S
S
Lemire
S
S
Stankovà
J
J
Rola-Pleszczynski
M
M
D000818:Animals; D019556:COS Cells; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D008969:Molecular Sequence Data; D018102:Receptors, Leukotriene B4 / Q000235:genetics / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D014162:Transfection
D007295:Inositol Phosphates; D018102:Receptors, Leukotriene B4; D019204:GTP-Binding Proteins