title: Impairment of long-term potentiation and paired-pulse facilitation in rat hippocampal dentate gyrus following developmental lead exposure in vivo.
abstract: Neonatal rats were exposed to lead from parturition to weaning via the milk of dams drinking 0.2% lead acetate solution. The alterations of long-term potentiation (LTP) and paired-pulse facilitation (PPF) of hippocampal dentate gyrus in adult rats (90-115 days) following developmental lead exposure were studied in vivo. Input/output (I/O) function, paired-pulse facilitation (PPF), excitatory postsynaptic potential (EPSP) and population spike (PS) amplitude were measured in the dentate gyrus (DG) in response to stimulation applied to the lateral perforant path. The results showed that LTP was induced in control rats with an average PS potentiation of 321.1+/-50.0% (n=18), which was significantly greater than the increase in PS potentiation (173.5+/-30.0%, n=17, p<0.001) in lead-exposed rats after tetanizing stimulation. The mean EPSP potentiation increased to 172.4+/-27.0% (n=18) in control and 138.8+/-21.4% (n=17) in lead-exposed rats after tetanizing stimulation. The lead-induced impairment of LTP of PS potentiation was more serious than that of EPSP potentiation. Following pairs stimulation of perforant fiber at 250 microA and an interpulse interval (IPI) of 10-1000 ms, the average peak facilitation of PS was 211.3+/-25.0% (n=13) in control and 187.7+/-23.0% (n=11) in lead-exposed rats. The average facilitation period duration of PS was 243.0+/-35.8 ms (n=13) in control and 138.0+/-24.4 ms (n=11) in lead-exposed rats. These results suggested that developmental lead exposure in neonatal rats caused impairments in LTP and PPF of hippocampal dentate gyrus.
authors.affiliation: School of Life Science, University of Science and Technology of China, Hefei, Anhui 230027, China. ruan@bioser.bio.ustc.edu.cn
authors.forename: D Y
authors.initials: DY
authors.lastname: Ruan
authors.forename: J T
authors.initials: JT
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Zhao
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Xu
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: W F
authors.initials: WF
authors.lastname: Zhao
mesh_terms: D000818:Animals; D018891:Dentate Gyrus / Q000187:drug effects* / Q000502:physiology*; D004558:Electric Stimulation / Q000379:methods; D005260:Female; D007854:Lead / Q000494:pharmacology*; D017774:Long-Term Potentiation / Q000187:drug effects* / Q000502:physiology; D008297:Male; D051381:Rats / Q000254:growth & development; D017208:Rats, Wistar
chemical_list: D007854:Lead