title: Effect of actinomycin D and cycloheximide on human sperm function.
abstract: The effects of actinomycin D and cycloheximide on human spermatozoal function were examined to investigate the potential transcriptional and translational activities of human sperm cell during capacitation/acrosome reaction. Treatment with actinomycin D significantly increased and treatment with cycloheximide decreased the acrosome reaction, and the penetration rates in the human sperm penetration of zona-free hamster oocytes assay (SPA). [35(S)]Methionine got incorporated into 3-9 de novo synthesized proteins present in the head and midpiece regions of the swim-up population of human sperm. Treatment with actinomycin D completely blocked and treatment with cycloheximide slightly reduced the synthesis of proteins. There seem to be some transcriptional and translational activities in the head and midpiece regions of human sperm during capacitation/acrosome reaction.
authors.affiliation: Department of Obstetrics and Gynecology, Medical College of Ohio, Toledo 43614-5809, USA. RNAZ@GEMINI.MCO.EDU
authors.forename: R K
authors.initials: RK
authors.lastname: Naz
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology; D000818:Animals; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology*; D003609:Dactinomycin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D014176:Protein Biosynthesis; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D003609:Dactinomycin; D003513:Cycloheximide