title: Fibrinogen adsorption and host tissue responses to plasma functionalized surfaces.
abstract: The physical and chemical characteristics of material surfaces are thought to play important roles in biomaterial-mediated tissue responses. To understand the importance of discrete biomaterial chemical characteristics in modifying host tissue responses, we constructed surfaces bearing different functional groups using radio frequency glow discharge plasma polymerization. Surfaces evaluated included those having high concentrations of -OH, -NH2, -CF3, and siloxyl groups. These surfaces and polyethylene terephthalate controls were used to assess the importance of particular physicochemical characteristics in surface:protein:cell interactions both in vitro and in vivo. The results obtained show that surface functionalities do significantly affect both the adsorption and "denaturation" of adsorbed fibrinogen (which is an important mediator of inflammatory responses to biomaterial implants). In addition, these surfaces provoke different degrees of acute inflammatory responses. Interestingly, the amounts of "denatured" fibrinogen that spontaneously accumulate on the individual surfaces correlate closely with the extent of biomaterial-mediated inflammation. These results suggest that surfaces that tend to "irreversibly" bind fibrinogen prompt greater acute inflammatory responses. Unexpectedly, all test surfaces except those bearing a siloxyl group engender relatively similar biomaterial-mediated fibrotic responses. Thus surface functionalities alone may not be sufficient to affect subsequent fibrotic responses.
authors.affiliation: Department of Pediatrics, Baylor College of Medicine, Texas Medical Center, Houston 77030-3498, USA.
authors.forename: L
authors.initials: L
authors.lastname: Tang
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: R B
authors.initials: RB
authors.lastname: Timmons
mesh_terms: D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials; D005340:Fibrinogen / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000209:etiology; D051379:Mice; D010949:Plasma; D019736:Prostheses and Implants; D011489:Protein Denaturation; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D005340:Fibrinogen