title: Corpeptins, new bioactive lipodepsipeptides from cultures of Pseudomonas corrugata.
abstract: The structure of the corpeptins, bioactive lipodepsipeptides produced in culture by Pseudomonas corrugata, the causal agent of tomato pith necrosis, has been determined. The combined use of FAB-mass spectrometry, NMR spectroscopy and chemical procedures has allowed us to assign the following primary structure to the peptide moiety: Dhb-Pro-Ala-Ala-Ala-Val-Val-Dhb-Hse-Val-alle-Dhp-Ala-Ala-Ala-Val-D hb-aThr-Ala-Dab-Ser-Ile with the terminal carboxy group closing a macrocyclic ring on the hydroxy group of the allo-threonine residue. The N-terminus is in turn acylated by 3-hydroxydecanoate in corpeptin A and by cis-3-hydroxy-5-dodecenoate in corpeptin B. Some preliminary data on the biological activity of corpeptins are included.
authors.affiliation: Istituto di Strutturistica Chimica G. Giacomello, CNR, Montelibretti, Rome, Italy.
authors.forename: M C
authors.initials: MC
authors.lastname: Emanuele
authors.forename: A
authors.initials: A
authors.lastname: Scaloni
authors.forename: P
authors.initials: P
authors.lastname: Lavermicocca
authors.forename: N S
authors.initials: NS
authors.lastname: Jacobellis
authors.forename: L
authors.initials: L
authors.lastname: Camoni
authors.forename: D
authors.initials: D
authors.lastname: Di Giorgio
authors.forename: P
authors.initials: P
authors.lastname: Pucci
authors.forename: M
authors.initials: M
authors.lastname: Paci
authors.forename: A
authors.initials: A
authors.lastname: Segre
authors.forename: A
authors.initials: A
authors.lastname: Ballio
mesh_terms: D000215:Acylation; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D003652:Decanoic Acids / Q000032:analysis; D006868:Hydrolysis; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D011549:Pseudomonas / Q000502:physiology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
chemical_list: D003470:Culture Media; D003652:Decanoic Acids; D010456:Peptides, Cyclic; C114641:corpeptin A; C114642:corpeptin B; C017552:myrmicacin