Ionic mechanisms of regional action potential heterogeneity in the canine right atrium.
Atrial action potential heterogeneity is a major determinant of atrial reentrant arrhythmias, but the underlying ionic mechanisms are poorly understood. To evaluate the basis of spatial heterogeneity in canine right atrial repolarization, we isolated cells from 4 regions: the crista terminalis (CT), appendage (APG), atrioventricular ring (AVR) area, and pectinate muscles. Systematic action potential (AP) differences were noted: CT cells had a "spike-and-dome" morphology and the longest AP duration (APD; value to 95% repolarization at 1 Hz, 270+/-10 ms [mean+/-SEM]); APG and pectinate muscle cells had intermediate APDs (180+/-3 and 190+/-3 ms, respectively; P<0.001 versus CT for each), with APG cells having a small phase 1; and AVR cells had the shortest APD (160+/-4 ms, P<0.001 versus other regions). The inward rectifier and the slow and ultrarapid delayed rectifier currents were similar in all regions. The transient outward K+ current was significantly smaller in APG cells, explaining their small phase 1 and high plateau. L-type Ca2+ current was greatest in CT cells and least in AVR cells, contributing to their longer and shorter APD, respectively. The E-4031-sensitive rapid delayed rectifier K+ current was larger in AVR cells compared with other regions. Voltage- and time-dependent current properties were constant across regions. We conclude that myocytes from different right atrial regions of the dog show systematic variations in AP properties and ionic currents and that the spatial variation in ionic current density may explain AP differences. Regional variation in atrial ionic currents may play an important role in atrial arrhythmia generation and may present opportunities for improving antiarrhythmic drug therapy.
Feng
J
J
Research Center, Montreal Heart Institute, Quebec, Canada.
Yue
L
L
Wang
Z
Z
Nattel
S
S
D000200:Action Potentials / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D016275:Atrial Function; D015220:Calcium Channels / Q000502:physiology; D004285:Dogs; D005260:Female; D006325:Heart Atria / Q000166:cytology; D007473:Ion Channels / Q000502:physiology*; D008297:Male; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000502:physiology
D015220:Calcium Channels; D007473:Ion Channels; D015221:Potassium Channels