title: Rapid diagnosis of pulmonary tuberculosis with the LCx Mycobacterium tuberculosis assay and comparison with conventional diagnostic techniques.
abstract: The LCx MTB amplification assay is a nucleic acid amplification test intended for the direct detection of Mycobacterium tuberculosis complex in respiratory specimens. We evaluated its performance on 2,001 consecutive respiratory specimens; 78 were culture positive for M. tuberculosis. Sensitivity, specificity, and positive and negative predictive values of this assay for all specimens compared to culture results were 88.5, 97.7, 60.5, and 99.5%, respectively. When referred to resolved clinical diagnosis of active tuberculosis, these values improved to 90.2, 98.4, 72.8, and 99.5%, respectively.
authors.affiliation: Infectious Disease Division, Bacteriology Laboratory, University Hospital Geneva, 1211 Geneva, Switzerland. Peter.Rohner@hcuge.ch
authors.forename: P
authors.initials: P
authors.lastname: Rohner
authors.forename: E I
authors.initials: EI
authors.lastname: Jahn
authors.forename: B
authors.initials: B
authors.lastname: Ninet
authors.forename: C
authors.initials: C
authors.lastname: Ionati
authors.forename: R
authors.initials: R
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: Auckenthaler
authors.forename: G E
authors.initials: GE
authors.lastname: Pfyffer
mesh_terms: D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005750:Gastric Juice / Q000382:microbiology; D006801:Humans; D007202:Indicators and Reagents; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014132:Trachea / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
chemical_list: D004269:DNA, Bacterial; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic