Effect of CDRI-85/287 on uterine estradiol and progesterone receptor levels/morphometric measurements during preimplantation period in rat.
Studies with 85/287, a potent nonsteroidal antiestrogen/antiimplantation agent were taken up. In this paper we report alterations in uterine morphometric measurements and estrogen/progesterone receptor levels under the influence of this compound. Results showed 32% decline in stromal absolute volume density on day 5 post-coitum (p.c.) only, whereas eosinophilic leucocyte number decreased both on days 3 and 5 p.c. (41%) in treated rat uterus. Epithelial mitotic activity showed complete cessation both in control and treated rats on day 5 p.c. Under the influence of the compound both cytosolic and nuclear estrogen receptor (ERc and ERn) levels decreased on day 3 p.c., but on day 5 p.c. ERn increased significantly. A significant increase was however noticed in progesterone nuclear receptors (PRn) on day 5 p.c. On the whole our studies showed overall significant changes in uterine morphometric measurement/estrogen and progesterone receptor levels during the preimplantation period in rat under the influence of compound 85/287, causing asynchrony of events and thus failure of implantation.
Gupta
S
S
Division of Endocrinology, Central Drug Research Institute, Lucknow, India.
Dhar
J D
JD
Dwivedi
A
A
Bansode
F W
FW
Chowdhury
S R
SR
Setty
B S
BS
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D001665:Binding Sites; D048429:Cell Size / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects; D004958:Estradiol / Q000097:blood; D004965:Estrogen Antagonists / Q000494:pharmacology; D005260:Female; D008297:Male; D000090204:Mesothelin; D008938:Mitosis / Q000187:drug effects; D009687:Nuclear Proteins / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology*; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley; D011959:Receptors, Estradiol / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014599:Uterus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D001578:Benzopyrans; D004965:Estrogen Antagonists; C000715529:Msln protein, rat; D009687:Nuclear Proteins; D010880:Piperidines; D011959:Receptors, Estradiol; D011980:Receptors, Progesterone; C066624:CDRI 85-287; D011374:Progesterone; D004958:Estradiol; D000090204:Mesothelin