title: Ultramicroanalysis of peptide profiles in biological samples using MALDI mass spectrometry.
abstract: In the past few years, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has emerged as a powerful tool for the direct analysis of peptide profiles contained in single neuroendocrine cells, tissue biopsies, as well as in releasates (after a simple sample clean-up). These studies were performed in both invertebrate (the pond snail) and vertebrate (xenopus and rat) species. The present article provides an overview of these data with a special emphasis on the sample handling required for each application.
authors.affiliation: Department of Molecular Neurobiology, Research Institute Neurosciences, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: C R
authors.initials: CR
authors.lastname: Jiménez
authors.forename: A L
authors.initials: AL
authors.lastname: Burlingame
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology; D005260:Female; D008195:Lymnaea; D009474:Neurons / Q000737:chemistry; D009490:Neurosecretory Systems / Q000737:chemistry / Q000166:cytology; D010058:Oviposition / Q000187:drug effects / Q000502:physiology; D010455:Peptides / Q000032:analysis*; D010904:Pituitary Gland, Posterior / Q000737:chemistry / Q000187:drug effects / Q000502:physiology; D051381:Rats; D012965:Sodium Chloride / Q000008:administration & dosage; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013873:Thionucleotides / Q000494:pharmacology; D014981:Xenopus
chemical_list: D010455:Peptides; D013873:Thionucleotides; C015929:8-((4-chlorophenyl)thio)cyclic-3',5'-AMP; D012965:Sodium Chloride; D000242:Cyclic AMP