title: Cytological aspiration of intraocular retinoblastoma in an 11-year-old boy.
abstract: Only a few cases of retinoblastomas in older children have been reported and the clinical diagnosis may be difficult. In case, fine-needle aspiration from an atypical retinal mass of an 11-yr-old boy was performed. The vitreous fluid was stained with Diff-Quik for an immediate cytological examination and the diagnosis of retinoblastoma was suggested. The rest of the specimen was separated into two parts. One was stained with May-Grünwald-Giemsa and the other was centrifuged, embedded in paraffin, and finally stained with hematoxylin-eosin-safran. The undifferentiated blue cells were associated with abundant necrotic debris and portions of capillaries with perivascular tumor cells around. The cytoplasm of the tumor cells was strongly stained with neuron-specific enolase antibody. The diagnosis of retinoblastoma was confirmed. The specimen of enucleation confirmed the diagnosis. In conclusion, cytological aspiration can categorically diagnose suspected intraocular tumors of older children in whom clinical and noninvasive investigations have failed to establish the diagnosis.
authors.affiliation: Department of Pathology, Hôpital de la Croix Rousse, Lyon, France.
authors.forename: M
authors.initials: M
authors.lastname: Decaussin
authors.forename: M D
authors.initials: MD
authors.lastname: Boran
authors.forename: M
authors.initials: M
authors.lastname: Salle
authors.forename: J D
authors.initials: JD
authors.lastname: Grange
authors.forename: L M
authors.initials: LM
authors.lastname: Patricot
authors.forename: F
authors.initials: F
authors.lastname: Thivolet-Bejui
mesh_terms: D001707:Biopsy, Needle; D002648:Child; D015353:Eye Enucleation; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D019572:Retinal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D012175:Retinoblastoma / Q000378:metabolism / Q000473:pathology* / Q000601:surgery
chemical_list: D010751:Phosphopyruvate Hydratase