Aldosterone stimulates Na,K-ATPase activity in basolateral membrane of rat kidney.
The changes of Na,K-ATPase activity and its regulation have been investigated in the renal cortex and its basolateral membrane of aldosterone-induced hypertensive rat. Ouabain-sensitive Na,K-ATPase activity and [3H]ouabain-binding site (Bmax) in the hypertensive rat were significantly increased than those in the control. The levels of Na,K-ATPase alpha 1- and beta 1-subunit mRNA of the renal cortex in hypertensive rat were more increased than those in the control, and their increases were repressed by actinomycin-D. These results suggest that the increase of Na,K-ATPase activities and ouabain binding sites in aldosterone-induced hypertensive rat may be correlated with transcriptional regulation of Na,K-ATPase gene expression.
Seok
J H
JH
Department of Pharmacology and Biochemistry, College of Medicine, Chungnam National University, Taejon, Korea. jhseok@hanbat.chungnam.ac.kr
Kim
J B
JB
Hong
J H
JH
Hur
G M
GM
Jeon
J R
JR
Lim
K
K
Hwang
B D
BD
Lee
J H
JH
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D003609:Dactinomycin / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic; D006973:Hypertension / Q000139:chemically induced / Q000201:enzymology*; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D010042:Ouabain / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D012333:RNA, Messenger; D003609:Dactinomycin; D000450:Aldosterone; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase