[Absence of HHV-8 virus detected in immature hemangiomas in infants].
BACKGROUND
The aim of our study was to search for the presence of HHV-8 DNA sequences in Biopsy specimens from hemangioma of the infancy.


METHODS
The study included 9 biopsies from hemangioma. DNA of human beta-globin gene and HHV-8 were searched for by PCR using specific primers. Amplified products were revealed after an hybridization with an internal probe digoxigenin-labelled.


RESULTS
Human beta-globin gene could be detected in all samples illustrating the absence of PCR inhibitor. HHV-8 could never be detected in samples analyzed.


CONCLUSIONS
Our study does not imply any causative role of HHV-8 in the pathogenesis of hemangioma. This result must be confirmed by serologic studies.
Dupin
N
N
Service de Dermato-Vénéréologie, Hôpital Tarnier-Cochin, Paris.
Enjolras
O
O
Wassef
M
M
Gorin
I
I
Gradadam
M
M
Huraux
J M
JM
Agut
H
H
Escande
J P
JP
D001706:Biopsy; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005914:Globins / Q000235:genetics; D006391:Hemangioma / Q000473:pathology / Q000821:virology*; D006566:Herpesviridae Infections / Q000150:complications* / Q000473:pathology; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*
D004279:DNA, Viral; D005914:Globins