title: Interleukin-1alpha and collagenase activity are elevated in chronic wounds.
abstract: Interleukin-1-alpha (IL-1alpha) is a member of a family of proinflammatory polypeptide mediators that has been shown in vitro to stimulate collagenase production. Collagenase is a proteolytic enzyme classified as one of the matrix metalloproteinases (MMP-1) that specifically recognizes and cleaves collagen. Therefore, the objective of this study was to compare the levels of these two proteins in chronic wounds as possible factors in the pathogenesis of chronic wounds. Fluids from 10 chronic wounds were collected before and after a 1-week treatment with a hydroactive dressing (Cutinova cavity). In addition, fluids were collected from 20 acute wounds for comparison. IL-1alpha and MMP-1 levels were quantified using sandwich ELISA. Collagenase activity was measured using a radiolabeled collagen as substrate. Clinically, the chronic wounds showed decreased area (-21.0 cm2) and reduced volume (-134.5 cm3) by 4 weeks after treatment with the hydroactive dressing. There were no significant differences in the protein concentrations between acute wound fluids (21.0 +/- 3.0 mg/ml) and chronic wound fluids before and after treatment with the hydroactive dressing (18.3 +/- 5.5 and 25.2 +/- 7.6 mg/ml, respectively). Levels of IL-1alpha in the acute wound fluids were low (0.019 pg/mg), whereas in the chronic wound fluid before treatment they had been significantly elevated (44.9 + 21.8 pg/mg). Following treatment with the hydroactive dressing, the IL-1alpha levels dropped to 10.3 + 3.3 pg/mg (p < 0.05). Collagenase activity was not detectable in acute wound fluid, elevated in pretreatment chronic wounds (12.9 + 3.4 units), and decreased in chronic wounds after treatment (11.4 + 3.3 units). This study correlated clinical healing of chronic wounds with biochemical changes in the ulcer microenvironment. As the chronic wounds began to heal, there was a significant decrease in the IL-1alpha levels and collagenase activity, thus suggesting that these two proteins may contribute to the lack of healing characteristic of chronic wounds.
authors.affiliation: Wound Healing Center, Division of Plastic and Reconstructive Surgery, at the Medical College of Virginia/Virginia Commonwealth University, Richmond 23298-0117, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Barone
authors.forename: D R
authors.initials: DR
authors.lastname: Yager
authors.forename: A L
authors.initials: AL
authors.lastname: Pozez
authors.forename: O O
authors.initials: OO
authors.lastname: Olutoye
authors.forename: M C
authors.initials: MC
authors.lastname: Crossland
authors.forename: R F
authors.initials: RF
authors.lastname: Diegelmann
authors.forename: I K
authors.initials: IK
authors.lastname: Cohen
mesh_terms: D002908:Chronic Disease; D017364:Collagenases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007375:Interleukin-1 / Q000378:metabolism*; D020781:Matrix Metalloproteinase 1; D009779:Occlusive Dressings; D003668:Pressure Ulcer / Q000503:physiopathology*; D013529:Surgical Wound Dehiscence / Q000503:physiopathology*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D007375:Interleukin-1; D017364:Collagenases; D020781:Matrix Metalloproteinase 1