Hair cell recovery in the chinchilla crista ampullaris after gentamicin treatment: a quantitative approach.
The mechanisms for hair cell recovery were investigated after intraortic application of 50 microg gentamicin into the perilymphatic space of the superior semicircular canal of the chinchilla. Histologic evaluation of one normal group and four posttreatment groups (7, 14, 28, and 56 days) was made with light and transmission electron microscopic techniques. The numeric changes of hair cells and supporting cells was quantified with the dissector technique. At 7 and 14 days after treatment, no type I hair cells were present, and 85% and 88% of type II hair cells were lost. Supporting cells decreased to 76% at 7 days, but they recovered to 91% at 14 days. Recovery of the epithelia was evident 28 days after treatment; 83% were type II hair cells, and 3% were type I hair cells. The supporting cell number remained close to normal (86%). Between 14 and 28 days after treatment, there was an increase of 1758 of type II hair cells, representing approximately 125 new hair cells per day. At the same time interval the number of supporting cells remained near normal. These results suggest that new hair cells might be the result of supporting cell mitotic division and differentiation.
López
I
I
Victor Goodhill Ear Center, Division of Head and Neck Surgery, UCLA School of Medicine, Los Angeles, California 90095-1624, USA.
Honrubia
V
V
Lee
S C
SC
Li
G
G
Beykirch
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D002682:Chinchilla; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005839:Gentamicins / Q000633:toxicity*; D018069:Hair Cells, Vestibular / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D012665:Semicircular Canals / Q000166:cytology / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D005839:Gentamicins