Specific and independent recognition of U3 and U5 att sites by human immunodeficiency virus type 1 integrase in vivo.
The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo.
Masuda
T
T
Department of Biodefense and Medical Virology, Kumamoto University School of Medicine, Kumamoto, Japan. tmasu.impt@med.tmd.ac.jp
Kuroda
M J
MJ
Harada
S
S
D001483:Base Sequence; D019427:HIV Integrase / Q000378:metabolism*; D016325:HIV Long Terminal Repeat; D015497:HIV-1 / Q000201:enzymology / Q000502:physiology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D012689:Sequence Homology, Nucleic Acid; D013379:Substrate Specificity; D016662:Virus Integration
D019427:HIV Integrase