title: IgE production from the nasal polyp tissue: comparison between atopic and non-atopic subjects.
abstract: OBJECTIVE
  To confirm the local production of IgE antibody from the nasal polyp tissue, and to evaluate the difference between atopics and non-atopics, nasal polyp tissues were taken from both 10 atopic and 10 non-atopic subjects. The tissue total IgE (tlgE) level was measured by enzyme-linked immunosorbent assay (ELISA) and serum tlgE level by radio-immunoassay. The tissue albumin level was measured by nephelometry, and serum albumin level by Bromocresol green method.
  
  
  RESULTS
  The polyp tissue tlgE/albumin as well as serum tlgE/albumin ratio were significantly higher in atopics than in non-atopics (p < 0.05), with no difference in the albumin level between the two groups (p > 0.05). Three non-atopic subjects had high polyp tissue tlgE/albumin (> 10). A significant correlation was noted between serum tlgE/albumin and polyp tlgE/albumin (r = 0.46, p = 0.04). The ratio of polyp tlgE/albumin to serum tlgE/albumin was greater than 1 in all of the non-atopic subjects and 7 of 10 atopic subjects.
  
  
  CONCLUSIONS
  These findings support the hypothesis that IgE antibody could be locally produced from the nasal polyp tissue of non-atopic subjects as well as atopic subjects. The possibility of an isolated local production of IgE antibody was suggested.
authors.affiliation: Department of Allergy and Clinical Immunology, Ajou University School of Medicine, Suwon, Korea.
authors.forename: H Y
authors.initials: HY
authors.lastname: Kim
authors.forename: D H
authors.initials: DH
authors.lastname: Nahm
authors.forename: K S
authors.initials: KS
authors.lastname: Suh
authors.forename: H S
authors.initials: HS
authors.lastname: Park
mesh_terms: D000418:Albumins / Q000032:analysis; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D001707:Biopsy, Needle; D046508:Culture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D009298:Nasal Polyps / Q000276:immunology* / Q000473:pathology; D011863:Radioimmunoassay; D012016:Reference Values
chemical_list: D000418:Albumins; D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E