Molecular cloning and characterization of a highly conserved chicken cellular nucleic acid binding protein cDNA.
A chicken cellular nucleic acid binding protein (cCNBP) cDNA was isolated from a chicken Con-A-stimulated immune cell library by differential screening. cCNBP is a Cys/Cys-His/Cys zinc finger DNA binding protein of unknown function. The chicken CNBP nucleotide and deduced amino acid sequence showed extraordinary sequence conservation (between 81-98% similarities) when compared to human, mouse and rat CNBP. The CNBP gene was shown to be a single copy and to cross-hybridize to human and mouse genomic DNA. A Northern blot analysis revealed cCNBP to be a constitutively expressed gene in a wide variety of tissues and to be differentially expressed in cultured chicken spleen and bursal cells after mitogen stimulation.
Ruble
D M
DM
University of Minnesota, Division of Animal Physiology, Department of Animal Science, St. Paul, MN 55108, USA.
Foster
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DN
D000818:Animals; D002478:Cells, Cultured; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D003208:Concanavalin A; D017124:Conserved Sequence; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013154:Spleen / Q000166:cytology
D003208:Concanavalin A; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C433227:CNBP protein, human; C499018:Cnbp protein, rat; C499012:Cnbp protein, mouse