title: Acetaminophen presystemic biotransformation vs bioavailability in therapeutical dosage range.
abstract: The influence of dose on the absorption and presystemic biotransformation of acetaminophen was studied in 15 healthy volunteers after administration of 3 different oral doses (250 mg, 500 mg, 750 mg) following a 3 x 3 Latin Square Design. The analytical method developed (High Performance Liquid Chromatography, HPLC) allows the rapid and simultaneous determination of acetaminophen and its major metabolites (its glucuronide and sulphate conjugates) by direct injection of urine samples. The statistical analysis did not reveal significant differences (alpha < 0.05) among treatments in the percentage of dose excreted and MRT and VRT values. Consequently, our results indicate that the absorption and biotransformation of acetaminophen is not affected by the dose in the usual therapeutical range.
authors.affiliation: Pharmacy and Pharmaceutics, Faculty of Pharmacy, University of Basque Country, Vitoria-Gasteiz, Spain.
authors.forename: A G
authors.initials: AG
authors.lastname: Goicoechea
authors.forename: J L
authors.initials: JL
authors.lastname: Vila-Jato
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D000328:Adult; D018712:Analgesics, Non-Narcotic / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D001682:Biological Availability; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008657:Metabolic Clearance Rate
chemical_list: D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen