Different genotypes causing indiscernible patterns of A expression on A(el) red blood cells as visualized by scanning immunogold electron microscopy.
OBJECTIVE
The published sequence of the weak. A subgroup Ael gene from Swedish individuals showed a G insertion in exon VII, causing a frameshift at codon 268 (the A1 gene has 353 codons). We wished to sequence exons VI and VII of two Norwegian Ael individuals and compare the expression of A substance on RBC from different Ael individuals.


METHODS
Exon VI and VII were amplified by PCR, cloned in M13 and sequenced. A structure expression on Ael RBC was studied by the immunogold technique.


RESULTS
In contrast to the Swedish Ael individuals, the two Norwegians had consensus A1 sequences in exon VI and VII. However, the patterns of A expression were indiscernible from the Swedish cases as visualized by immunogold labeling in SEM. In both cases, a few (1-2%) RBC were very strongly labeled, some were weakly labeled and the majority (95%) were unlabeled.


CONCLUSIONS
Although some Ael individuals have an inserted nucleotide in exon VII of the ABO gene, others have consensus A1 sequence in exon VI and VII. However, we could not find any differences in phenotype by immunogold labeling in SEM.
Hansen
T
T
Ullevål University Hospital, Department of Immunology and Transfusion Medicine, Oslo, Norway.
Namork
E
E
Olsson
M L
ML
Chester
M A
MA
Heier
H E
HE
D000017:ABO Blood-Group System / Q000096:biosynthesis* / Q000235:genetics; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004910:Erythrocyte Membrane / Q000737:chemistry* / Q000648:ultrastructure; D005091:Exons / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D005838:Genotype; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D016253:Microscopy, Immunoelectron; D016254:Mutagenesis, Insertional; D017350:N-Acetylgalactosaminyltransferases / Q000737:chemistry / Q000235:genetics*; D009664:Norway; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D013548:Sweden
D000017:ABO Blood-Group System; D011993:Recombinant Fusion Proteins; D017350:N-Acetylgalactosaminyltransferases; C003742:UDPgalactosamine-galactose acetylgalactosaminyltransferase