Promoter-independent regulation of cell-specific dopamine receptor expression.
Here we describe the construction of recombinant adenoviruses expressing dopamine D2 and D4 receptors, and their ability to mediate high levels of heterologous expression in a variety of cell types in vitro and in vivo for at least 7 days post infection. These experiments demonstrated that maximum receptor expression is achieved generally within 24 h and remains constant thereafter. Maximum expression levels were highly variable between cell lines and dependent on infection efficiency and promoter strength. Correction for these two variables revealed differences in relative expression levels between cell lines varying by two orders of magnitude. Our results indicate that in addition to gene transcription, post-transcriptional mechanisms play a dominant role in determining dopamine receptor levels in this system.
Knapp
M
M
Laboratory of Molecular Neurobiology, Clarke Institute of Psychiatry, University of Toronto, Ont., Canada.
Wong
A H
AH
Schoots
O
O
Guan
H C
HC
Van Tol
H H
HH
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis / Q000235:genetics*; D017448:Receptors, Dopamine D2 / Q000096:biosynthesis / Q000235:genetics*; D013045:Species Specificity
D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2