[Synthesis and biological properties of artificial antigens based on 280-289 fragment of beta2-gpI].
A series of artificial antigens were synthesized on the basis of the FC(Acm)KNKEKKC(Acm)S peptide from the beta 2-glycoprotein I sequence: lipophilic analogues, the peptide-BSA conjugate, and multiple antigen peptide (MAP) containing eight copies of the peptide on an oligolysyl core. The solid phase method for acylation of the peptide with fatty acids and the HPLC analysis of the acylpeptides were described. Antigenic properties of the resulting compounds were evaluated by CL-ELISA.
Pal'keeva
M E
ME
Russian Cardiological Scientific Center, Russian Ministry of Health, Moscow, Russia. pal'keeva.csc@ias.adonis.msk.ru
Sidorova
M V
MV
Molokoedov
A S
AS
Kuznetsova
T V
TV
Tishchenko
V A
VA
Kobylianskiĭ
A G
AG
Bespalova
Zh D
ZhD
Nasonov
E L
EL
Evstigneeva
R P
RP
D000215:Acylation; D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000737:chemistry / Q000276:immunology; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000096:biosynthesis* / Q000737:chemistry / Q000276:immunology; D001053:Apolipoproteins / Q000737:chemistry* / Q000276:immunology; D002308:Cardiolipins / Q000737:chemistry* / Q000276:immunology; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D008239:Lysine / Q000737:chemistry; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology; D010446:Peptide Fragments / Q000737:chemistry* / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000737:chemistry; D053482:beta 2-Glycoprotein I
D017153:Antibodies, Anticardiolipin; D000936:Antigen-Antibody Complex; D000941:Antigens; D001053:Apolipoproteins; D002308:Cardiolipins; D005227:Fatty Acids; D006023:Glycoproteins; D009842:Oligopeptides; D010446:Peptide Fragments; D053482:beta 2-Glycoprotein I; D012710:Serum Albumin, Bovine; D008239:Lysine