Immunohistochemical localization of peroxisomal enzymes in developing rat kidney tissues.
We studied the developmental changes in the localization of peroxisome-specific enzymes in rat kidney tissues from embryonic Day 16 to postnatal Week 10 by immunoblot analysis and immunohistochemistry, using antibodies for the peroxisomal enzymes catalase, d-amino acid oxidase, l-alpha-hydroxyacid oxidase (isozyme B), and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein. Peroxisomal enzymes were detected in the neonatal kidney by immunoblot analysis and their amount increased with kidney development. By light microscopic immunohistochemistry, they were first localized in a few proximal tubules in the juxtamedullary cortex of 18-day embryos. The distribution of proximal tubules positive for them expanded towards the superficial cortex with development. The full thickness of the cortex became positive for the staining by 14 days after birth. Peroxisomes could be detected by electron microscopy in structurally immature proximal tubules in 18-day embryos. Their size increased and the ultrastructure of subcompartments became clear with continuing development of proximal tubules. These results show that peroxisomal enzymes appear in the immature proximal tubules in the kidney of embryos and that the ultrastructure of the peroxisomes and localization of the peroxisomal enzymes develop along with the maturation of proximal tubules and kidney tissues.
Johkura
K
K
Department of Anatomy and Cell Biology, Shinshu University School of Medicine, Matsumoto, Japan.
Usuda
N
N
Liang
Y
Y
Nakazawa
A
A
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003605:D-Amino-Acid Oxidase / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D005260:Female; D015151:Immunoblotting; D007150:Immunohistochemistry; D007535:Isomerases; D007668:Kidney / Q000196:embryology / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000201:enzymology*; D008854:Microscopy, Electron; D009097:Multienzyme Complexes / Q000378:metabolism; D063994:Peroxisomal Bifunctional Enzyme; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D009097:Multienzyme Complexes; D015094:3-Hydroxyacyl CoA Dehydrogenases; D002374:Catalase; D003605:D-Amino-Acid Oxidase; D004746:Enoyl-CoA Hydratase; D063994:Peroxisomal Bifunctional Enzyme; D007535:Isomerases