Mutagenicity and DNA-damaging activity of decomposed products of food colours under UV irradiation.
Five synthetic food colours Food Red Nos 3, 40 and 102 and Food Blue Nos 1 and 2, and their UV irradiated products were tested for mutagenic activity by means of the Ames test using Salmonella typhimurium strains TA98 and TA100. Food colours were irradiated with UV light for 14 days. Food Red Nos 3, 40 and 102 and Food Blue No. 1 were non-mutagenic before and after irradiation. UV irradiated products of Food Blue No. 2 were mutagenic in TA98 with or without S-9 mix. The mutagenic activity increased with increasing irradiation period, reached maximum potency on day 6, and then decreased. Moreover, Food Blue No. 2 showed DNA-damaging activity after 14 days of irradiation in rec-assay using Bacillus subtilis strains H17 and M45. The capillary electrophoresis was applied for the analysis of UV irradiated products of Food Blue No. 2. The original peak of Food Blue No. 2 was decomposed into seven peaks after UV irradiation.
Ozaki
A
A
Faculty of Human Life Science, Osaka City University, Osaka, Japan.
Kitano
M
M
Itoh
N
N
Kuroda
K
K
Furusawa
N
N
Masuda
T
T
Yamaguchi
H
H
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics; D001557:Benzenesulfonates / Q000528:radiation effects / Q000633:toxicity; D004396:Coloring Agents / Q000528:radiation effects / Q000633:toxicity; D004249:DNA Damage; D019075:Electrophoresis, Capillary; D005505:Food Coloring Agents / Q000528:radiation effects* / Q000633:toxicity*; D009152:Mutagenicity Tests; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D014466:Ultraviolet Rays
D001557:Benzenesulfonates; D004396:Coloring Agents; D005505:Food Coloring Agents; C006796:brilliant blue