Increased mRNA levels of transforming growth factor-beta1 and monocyte chemoattractant protein-1 in ulcer relapse caused by interleukin-1beta in rats.
This study investigated the mRNA expression of transforming growth factor-beta1 (TGF-beta1) and monocyte chemoattractant protein-1 (MCP-1) in rat gastric tissues in which ulcers had relapsed due to interleukin-1beta (IL-1beta) administration. Rats with healed ulcers were administered IL-1beta (1 microg/kg) and killed after 0, 12, 24, or 48 hr. Both TGF-beta1 and MCP-1 mRNA levels were increased in the scarred gastric tissues at 24 hr (fourfold), when ulcers had not relapsed. Furthermore, the expression of these genes also increased in the ulcerated gastric tissues at 48 hr (fivefold), when 90% of healed ulcers had relapsed. On the other hand, the number of macrophages that had infiltrated the scarred gastric tissues at 24 hr was two times higher than that at 0 hr. At 48 hr, the number of macrophages that had infiltrated gastric tissues in which ulcers had relapsed was similar to that at 24 hr. Thus, TGF-beta1 and MCP-1 may be implicated in the macrophage infiltration, thereby leading to ulcer relapse due to IL-1beta.
Tominaga
K
K
Third Department of Internal Medicine, Osaka City University Medical School, Osaka, Japan.
Arakawa
T
T
Watanabe
T
T
Tanaka
M
M
Takaishi
O
O
Fujiwara
Y
Y
Fukuda
T
T
Higuchi
K
K
Kim
S
S
Yamasaki
K
K
Iwao
H
H
Kobayashi
K
K
Kuroki
T
T
D000818:Animals; D015152:Blotting, Northern; D018932:Chemokine CCL2 / Q000235:genetics / Q000378:metabolism*; D004195:Disease Models, Animal; D005753:Gastric Mucosa / Q000378:metabolism*; D005786:Gene Expression Regulation; D007375:Interleukin-1; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012008:Recurrence; D013276:Stomach Ulcer / Q000378:metabolism*; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism*
D018932:Chemokine CCL2; D007375:Interleukin-1; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta