Immunization of cows with ferric enterobactin receptor from coliform bacteria.
The serum and milk immunoglobulin (Ig) G responses of lactating dairy cows were determined following immunization with ferric enterobactin receptor FepA. Escherichia coli 471 was cultured in iron-depleted medium, and outer membrane proteins were extracted by 2% N-lauroylsarcosine sodium salt and 2% Triton X-100. The FepA was isolated from the outer membrane proteins by ion-exchange chromatography. Twenty cows were assigned to four treatment groups of 5 cows blocked by breed and days in milk. Treatment groups were vaccinated with 100 micrograms of FepA, 500 micrograms of FepA, Escherichia coli J5 bacterin, or sterile phosphate-buffered saline. Primary immunization was at approximately 200 d in milk, and booster immunizations were given 14 and 28 d later. Serum and whey IgG titers to FepA in cows vaccinated with FepA were significantly higher than those from cows vaccinated with either E. coli J5 bacterin or phosphate-buffered saline. Serum and whey IgG titers to FepA were elevated by 14 d in cows vaccinated with FepA. Significant differences were not observed between doses of FepA. The degree of cross-reactivity of purified IgG from cows vaccinated with FepA to E. coli and Klebsiella pneumoniae isolates was significantly higher than that to a control isolate that lacked FepA production. Immunization with FepA elicited an immunological response in serum and milk.
Lin
J
J
Department of Animal Sciences, Ohio State University, Ohio Agricultural Research and Development Center, Wooster 44691, USA.
Hogan
J S
JS
Aslam
M
M
Smith
K L
KL
D000645:Ammonium Sulfate; D000818:Animals; D000918:Antibody Specificity; D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control*; D011232:Chemical Precipitation; D004926:Escherichia coli / Q000276:immunology*; D005260:Female; D007114:Immunization / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D007711:Klebsiella pneumoniae / Q000276:immunology; D011956:Receptors, Cell Surface
D001425:Bacterial Outer Membrane Proteins; D001428:Bacterial Vaccines; D002352:Carrier Proteins; D007074:Immunoglobulin G; D011956:Receptors, Cell Surface; C029489:enterobactin receptor; D000645:Ammonium Sulfate