Two restriction endonucleases in Selenomonas ruminantium subsp. lactilytica.
Crude protein extract from a recently isolated ruminal bacterium identified as Selenomonas ruminantium subsp. lactilytica specifically cleaved DNA. This ability was due to the presence of two site-specific restriction endonucleases. Sr/I, a NaeI schizomer, recognizes the 5'-GCCGGC-3' sequence. Sr/II, a NsiI schizomer, recognizes 5'-ATGCAT-3'.
Pristas
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Institute of Animal Physiology, Slovak Academy of Sciences, Soltesovej, Kosice, Slovakia. pristas@mail.saske.sk
Fliegerová
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Javorský
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D000818:Animals; D004269:DNA, Bacterial / Q000302:isolation & purification; D015252:Deoxyribonucleases, Type II Site-Specific / Q000302:isolation & purification / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology* / Q000235:genetics; D012417:Rumen / Q000382:microbiology
D004269:DNA, Bacterial; C060630:endodeoxyribonuclease NaeI; C048503:ATGCAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific