Genetically modified ruminal bacteria protect sheep from fluoroacetate poisoning.
Four strains of Butyrivibrio fibrisolvens, transformed with a gene encoding fluoroacetate dehalogenase, maintained a combined population of 10(6) to 10(7) cells ml-1 in the rumens of test sheep. Five inoculated sheep showed markedly reduced toxicological symptoms after fluoroacetate poisoning when behavioral, physiological, and histological effects were compared with those of five uninoculated control sheep.
Gregg
K
K
Rumen Biotech, Murdoch University, Murdoch, Western Australia 6150, Australia. K.Gregg@murdoch.edu.au
Hamdorf
B
B
Henderson
K
K
Kopecny
J
J
Wong
C
C
D000818:Animals; D005463:Fluoroacetates / Q000506:poisoning*; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000201:enzymology / Q000235:genetics*; D006867:Hydrolases / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D011041:Poisoning / Q000517:prevention & control / Q000662:veterinary; D011994:Recombinant Proteins / Q000378:metabolism; D012417:Rumen / Q000382:microbiology*; D012756:Sheep; D012757:Sheep Diseases / Q000139:chemically induced* / Q000517:prevention & control; D014169:Transformation, Bacterial
D005463:Fluoroacetates; D011994:Recombinant Proteins; C014815:fluoroacetic acid; D006867:Hydrolases; C042472:fluoroacetate-specific dehalogenase