title: PRAME, a gene encoding an antigen recognized on a human melanoma by cytolytic T cells, is expressed in acute leukaemia cells.
abstract: Gene PRAME was found to encode an antigen recognized on a human melanoma cell line by an autologous cytolytic T-lymphocyte clone. This gene is expressed at a high level in a very large fraction of tumours, such as melanomas, non-small-cell lung carcinomas, sarcomas, head and neck tumours and renal carcinomas. It is therefore a candidate for tumour immunotherapy even though some low expression is found in certain normal tissues. We tested by RT-PCR the expression of PRAME on more than 250 bone marrow or blood samples from patients with a haematological malignancy. Approximately 25% of the acute leukaemia samples were positive. Remarkably, all acute myeloblastic leukaemias that carried the chromosomal translocation t(8;21), which fuses the genes AML1 and ETO, expressed PRAME at a high level.
authors.affiliation: Cellular Genetics Unit, Université Catholique de Louvain, Brussels, Belgium.
authors.forename: N
authors.initials: N
authors.lastname: van Baren
authors.forename: H
authors.initials: H
authors.lastname: Chambost
authors.forename: A
authors.initials: A
authors.lastname: Ferrant
authors.forename: L
authors.initials: L
authors.lastname: Michaux
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: I
authors.initials: I
authors.lastname: Millard
authors.forename: D
authors.initials: D
authors.lastname: Olive
authors.forename: T
authors.initials: T
authors.lastname: Boon
authors.forename: P G
authors.initials: PG
authors.lastname: Coulie
mesh_terms: D000208:Acute Disease; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D002869:Chromosome Aberrations; D015870:Gene Expression; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008545:Melanoma / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D012334:RNA, Neoplasm