title: Short term response of circulating leptin to feeding and fasting in man: influence of circadian cycle.
abstract: OBJECTIVE
  To investigate whether acute feeding induces changes in circulating leptin levels in humans and whether these changes vary according to nycthemeral cycle.
  
  
  METHODS
  First experiment. Eighteen male subjects were given a fatty meal at 08.00 h. Blood sampling was performed for 10 h following this meal. Second experiment. Thirteen male subjects were given either a mixed meal or remained fasting either at night (starting at 01.00 h) or during the day (starting at 13.00 h). Blood samples were drawn every hour for a period of 8 h.
  
  
  RESULTS
  First experiment. Serum leptin levels increased progressively from a mean (s.d.) baseline of 3.8 +/- 2.2 ng/ml to a value of 4.5 +/- 2.7 ng/ml (P < 0.01) 8 h after the fatty meal. Second experiment. During the day, serum leptin levels increased progressively from 2.65 +/- 1.7 to 3.34 +/- 2.2 ng/ml (P < 0.001) 6 h after the test-meal and decreased from 2.68 +/- 1.5 to 1.9 +/- 1.1 ng/ml (P < 0.001) 8 h after the beginning of the fasting experiment. Similar results were obtained at night. No statistically significant differences in leptin levels were observed between day and night sessions in response to feeding (mean area under the curve: 3.0 +/- 4.1 vs 4.1 +/- 4.1 ng/ml) and fasting (-2.9 +/- 2.2 vs -1.5 +/- 2.2 ng/ml).
  
  
  CONCLUSIONS
  In two independent experiments, human serum leptin levels increase following food intake. This response is not influenced by nycthemeral cycle.
authors.affiliation: INSERM U-325 and Département d'Athérosclérose, Institut Pasteur de Lille, France.
authors.forename: J
authors.initials: J
authors.lastname: Dallongeville
authors.forename: B
authors.initials: B
authors.lastname: Hecquet
authors.forename: P
authors.initials: P
authors.lastname: Lebel
authors.forename: J L
authors.initials: JL
authors.lastname: Edmé
authors.forename: C
authors.initials: C
authors.lastname: Le Fur
authors.forename: J C
authors.initials: JC
authors.lastname: Fruchart
authors.forename: J
authors.initials: J
authors.lastname: Auwerx
authors.forename: M
authors.initials: M
authors.lastname: Romon
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D019540:Area Under Curve; D001786:Blood Glucose / Q000378:metabolism; D002940:Circadian Rhythm / Q000502:physiology*; D004041:Dietary Fats / Q000008:administration & dosage; D004435:Eating / Q000502:physiology*; D005215:Fasting / Q000502:physiology*; D006801:Humans; D007328:Insulin / Q000097:blood; D020738:Leptin; D008297:Male; D011506:Proteins / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007328:Insulin; D020738:Leptin; D011506:Proteins; D014280:Triglycerides