Characterization of an element positively regulating the transcription of MSSP gene-2 which encodes C-MYC binding proteins.
MSSP gene-2 encodes at least three alternative splicing products, MSSP-1, MSSP-2, and Scr2, which have been implied to function as factors regulating DNA replication, transcription, apoptosis induction, and cell-cycle movement, via the interaction with C-MYC. We have previously shown that the Mup1 sequence from -474 to -440 in the region required for transactivation of the MSSP gene-2 was protected from DNaseI digestion in vitro. Here, we report that the Mup1 sequence carries a positive transcriptional activity, and that the activity was lost by point mutations that inhibited the formation of specific nucleoprotein complexes on the sequence. In Southwestern analyses, two proteins of 38 and 62kDa directly interacted with the Mup1 sequence.
Fujimoto
M
M
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo 060, Japan.
Iguchi-Ariga
S M
SM
Ariga
H
H
D017398:Alternative Splicing; D001483:Base Sequence; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D003850:Deoxyribonuclease I; D004742:Enhancer Elements, Genetic; D006367:HeLa Cells; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins; D015533:Transcriptional Activation; D014162:Transfection
D017931:DNA Primers; D004268:DNA-Binding Proteins; D016271:Proto-Oncogene Proteins c-myc; C086384:RBMS1 protein, human; D016601:RNA-Binding Proteins; D004247:DNA; D008156:Luciferases; D003850:Deoxyribonuclease I