Localization of a T-cell epitope of superantigen toxic shock syndrome toxin 1 to residues 125 to 158.
Toxic shock syndrome toxin 1 (TSST-1) is a member of the staphylococcal enterotoxin superantigen family. So far, little is known about T-cell epitopes on superantigens. In this study, we developed an improved method for localizing T-cell epitopes on superantigens that involved synthetic peptides plus costimulation by CD28 or phorbol myristate acetate. Using this method, we localized a T-cell epitope to a 34-residue region, TSST-1 (residues 125 to 158), which possessed only two of four TSST-1-targeted beta-chain variable element (Vbeta) specificities of T-cell receptors in humans and mice, human Vbeta2 and murine Vbeta15.
Hu
W G
WG
Department of Immunology, The Third Military Medical University, Chongqing 400038, People's Republic of China. wghu@hotmail.com
Zhu
X H
XH
Wu
Y Z
YZ
Jia
Z C
ZC
D000818:Animals; D018106:CD28 Antigens / Q000276:immunology; D004768:Enterotoxins / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D051379:Mice; D010446:Peptide Fragments / Q000276:immunology*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013755:Tetradecanoylphorbol Acetate / Q000276:immunology
D018106:CD28 Antigens; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; D016693:Receptors, Antigen, T-Cell, alpha-beta; D018089:Superantigens; C114928:toxic shock syndrome toxin 1 (101-158); C114927:toxic shock syndrome toxin 1 (125-158); D013755:Tetradecanoylphorbol Acetate