Specificity of Pseudomonas aeruginosa serralysin revisited, using biologically active peptides as substrates.
The characterization of the specificity of alkaline protease from Pseudomonas aeruginosa has not yet been clearly defined. Some previous results suggested that its specificity was influenced more by amino acids far from the hydrolyzed peptide bond, than by amino acids in P1 or P'1 position. From other data, it was a C-(COOH)-type endoprotease where the preferential amino acid in P1 position was an arginine residue. We have studied the hydrolysis of several biologically active peptides. Many various sites of cleavage have been characterized but no arginine in P1 position was found, despite the presence of arginine in the peptide sequence. In fact P1 and P'1 position could be occupied by various amino acids. It seems unlikely that Pseudomonas alkaline protease may only be considered as a protease specific to arginine in P1 position. On the other hand, we have observed that increase of the peptide chain length led to an important increase of the hydrolysis rate, suggesting an extended number of subsites.
Louis
D
D
Laboratoire de Biochimie Analytique et de Synthese Bioorganique, Université Claude Bernard-Lyon 1, 43 Boulevard 11 Nov. 1918, 69622 Villeurbanne Cedex, France.
Bernillon
J
J
Wallach
J M
JM
D000803:Angiotensin I / Q000378:metabolism; D000804:Angiotensin II / Q000378:metabolism; D001120:Arginine / Q000737:chemistry; D001426:Bacterial Proteins / Q000737:chemistry; D010450:Endopeptidases / Q000378:metabolism; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D013058:Mass Spectrometry; D008666:Metalloendopeptidases / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000201:enzymology*; D013373:Substance P / Q000378:metabolism; D013379:Substrate Specificity
D001426:Bacterial Proteins; D005338:Fibrin Fibrinogen Degradation Products; D009842:Oligopeptides; D010446:Peptide Fragments; C026893:fibrinogen peptide 6A; D000804:Angiotensin II; D013373:Substance P; D000803:Angiotensin I; D001120:Arginine; C049554:glycyl-arginyl-glycyl-aspartyl-serine; D010450:Endopeptidases; D008666:Metalloendopeptidases; C095666:serralysin