Genotyping of Chlamydia trachomatis in urine specimens will facilitate large epidemiological studies.
The serovars of Chlamydia trachomatis-positive urine specimens (n = 81; as detected by PCR and ligase chain reaction) were successfully analyzed in 94% of cases by omp1 PCR-based RFLP analysis. The use of urine specimens and this simple and sensitive typing method will greatly facilitate epidemiological studies of C. trachomatis serovar distribution in asymptomatic C. trachomatis infections in both females and males.
Morré
S A
SA
Section of Molecular Pathology, Department of Pathology, University Hospital Vrije Universiteit, 1081 HV, Amsterdam, The Netherlands.
Moes
R
R
Van Valkengoed
I
I
Boeke
J P
JP
van Eijk
J T
JT
Meijer
C J
CJ
Van den Brule
A J
AJ
D002353:Carrier State / Q000175:diagnosis / Q000652:urine; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000652:urine; D002692:Chlamydia trachomatis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005260:Female; D006801:Humans; D008297:Male; D009426:Netherlands; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012680:Sensitivity and Specificity; D012703:Serotyping; D014556:Urine / Q000382:microbiology