Sialyllactose occurs as free lactones in ovine colostrum.
Three sialyl oligosaccharide fractions were separated from ovine colostrum by gel filtration, anion exchange chromatography and normal-phase HPLC. They were characterized by 1H-NMR spectrometry as follows: Neu5Acalpha2-->3Galbeta1-->4Glc, Neu5Gcalpha2-->6Galbeta1-->4Glc and three forms of Neu5Gcalpha2-->3Galbeta1-->4Glc, namely Neu5Gcalpha2-->3Galbeta1-->4Glc itself, its lactone derivative between the carboxyl group of Neu5Gc and Gal OH-2 and another lactone derivative between the carboxyl group and Gal OH-4. In this study, Neu5Gc-lactose lactones, in their free form, have been isolated for the first time from any natural sources including milk or colostrum.
Nakamura
T
T
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Hokkaido, Obihiro, 080-8555, Japan. nakamura@obihiro.ac.jp
Urashima
T
T
Nakagawa
M
M
Saito
T
T
D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D007783:Lactones / Q000032:analysis* / Q000737:chemistry; D007785:Lactose / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis / Q000302:isolation & purification; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis* / Q000302:isolation & purification
D007783:Lactones; D009844:Oligosaccharides; D012794:Sialic Acids; C000020:N-acetylneuraminoyllactose; D007785:Lactose