title: Prostaglandin and fatty acid modulation of Escherichia coli O157 phagocytosis by human monocytic cells.
abstract: Phagocytosis by human monocytes is an important primary survival mechanism particularly during bacterial infection. However, the processes that control the events and mediators involved in the activation of monocytes and their impact on the phagocytosis of bacteria are poorly understood. The effect of bacterial endotoxin, interleukin-1 beta (IL-1 beta), fatty acids and prostaglandin E2 (PGE2) on the phagocytosis of fluoroscein isothiocyanate (FITC)-labelled Escherichia coli (O157) by human blood monocytes and U937 cells was studied by flow cytometry. Endotoxin increased the phagocytosis of labelled bacteria by both monocytes and U937 cells. IL-1 beta and the polyunsaturated fatty acids; dihomo-gamma-linolenic and arachidonic acids also increased the phagocytic activity of both monocytes and U937 cells. In contrast, PGE2 suppressed phagocytosis in a concentration-dependent manner. The cyclo-oxygenase inhibitor, ketoprofen, further enhanced the increased phagocytic activity in the presence of endotoxin and interleukin-1 (IL-1) indicating suppression by endogenous prostaglandins. This was confirmed by the data which showed that lipopolysaccharide (LPS) and IL-1 increased PGE2 release and ketoprofen inhibited release. Endotoxin and fatty acids increased IL-1 beta release also, whereas PGE2 inhibited release. The data suggest that phagocytic activity may be linked to changes in IL-1 levels. The data presented in this study also suggest that monocyte phagocytosis in the course of bacterial infection would be altered during pathophysiological events which result in elevation of extracellular fatty acids.
authors.affiliation: Department of Immunology, University of Strathclyde, Glasgow, UK.
authors.forename: J
authors.initials: J
authors.lastname: Davidson
authors.forename: A
authors.initials: A
authors.lastname: Kerr
authors.forename: K
authors.initials: K
authors.lastname: Guy
authors.forename: D
authors.initials: D
authors.lastname: Rotondo
mesh_terms: D018929:Cell Culture Techniques; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology*; D004306:Dose-Response Relationship, Immunologic; D019453:Escherichia coli O157 / Q000276:immunology*; D005227:Fatty Acids / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate; D006801:Humans; D007375:Interleukin-1 / Q000276:immunology; D007660:Ketoprofen / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis / Q000187:drug effects*; D014407:Tumor Cells, Cultured
chemical_list: D016861:Cyclooxygenase Inhibitors; D005227:Fatty Acids; D007375:Interleukin-1; D008070:Lipopolysaccharides; D007660:Ketoprofen; D016650:Fluorescein-5-isothiocyanate; D015232:Dinoprostone