title: Distribution of insertion sequence IS200 among different clonal lines of the related Salmonella serotypes Livingstone and Eimsbuettel.
abstract: The copy number and genetic location of IS200 have provided evidence of strain relatedness in many serotypes of Salmonella. In this study, 100 isolates of the related serotypes Livingstone (6,7:d:l,w) and Eimsbuettel (6,7,14:d:l,w), representing 10 ribotype/biotype (RT/BT) groups isolated from human and non-human sources in seven countries over a 26-year period, were examined for their IS200 profiles. The distribution of IS200 in strains of these serotypes was limited, being present in all 53 isolates of ribotype 1 (RT1) and its variant type RT6, in one of five isolates of RT5 but in none of 42 isolates of RTs 2, 3 or 4. Although the seven IS200 profiles identified in RT1 isolates were of little value for further discrimination within different biotype groups, they were extremely valuable for confirming serotype: isolates of RT1/BT8/IS200 profile A (or its variants) and those of RT1/BT3/IS200 profile B (or its variants) were almost invariably associated with serotypes Livingstone and Eimsbuettel, respectively.
authors.affiliation: Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital.
authors.forename: P B
authors.initials: PB
authors.lastname: Crichton
authors.forename: D C
authors.initials: DC
authors.lastname: Old
authors.forename: H F
authors.initials: HF
authors.lastname: Morphy
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D012475:Salmonella / Q000145:classification* / Q000235:genetics; D012480:Salmonella Infections / Q000382:microbiology; D012481:Salmonella Infections, Animal / Q000382:microbiology; D012703:Serotyping
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial