title: Molecular characterization of the Notch homologue from the Australian sheep blowfly, Lucilia cuprina.
abstract: The Drosophila melanogaster Notch gene product as a receptor of intercellular signals and is central to cell fate specification. The Scalloped wings (Scl) gene is the homologue of Notch in the Australian sheep blowfly, Lucilia cuprina. An allele of Scl is thought to be involved in the modification of Darwinian fitness and bristle asymmetry in flies resistant to organophosphorous chemicals (OPs). As a first step towards the testing of this hypothesis we cloned and sequenced Scl. A full-length cDNA segment representing the mRNA of Scl is 8503 bp and encodes a protein of 2653 amino acids, which shares 73.6% identity with Notch. All functional motifs including EGF-like repeats, LNR repeats, cdc 10/ankyrin repeats, opa and PEST elements are present in the same order as in Notch and the sequence identities peak in these motifs. With respect to genomic structure, intron/exon boundaries are conserved but, in most cases, the Scl introns are larger. Sequence analysis of the upstream genomic region reveals that the gene has a TATA-less promoter. Consistent with a central role in embryogenesis and imaginal development, high levels of Scl expression were detected in the early embryonic and pupal stages.
authors.affiliation: Department of Genetics, University of Melbourne, Parkville, Victoria, Australia. z.chen@genetics.unimelb.edu.au
authors.forename: Z
authors.initials: Z
authors.lastname: Chen
authors.forename: T
authors.initials: T
authors.lastname: Newsome
authors.forename: J A
authors.initials: JA
authors.lastname: McKenzie
authors.forename: P
authors.initials: P
authors.lastname: Batterham
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004175:Diptera / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D007438:Introns; D007814:Larva / Q000235:genetics / Q000254:growth & development; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D009943:Organophosphorus Compounds; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D008562:Membrane Glycoproteins; D009943:Organophosphorus Compounds