Phenotypic differences between diabetes-prone BB rat sublines cosegregate with loci on chromosomes X and 10.
BB rat sublines, all developing an insulin-dependent diabetes, differ in several phenotypic traits and in the genotype. To study the genetic basis of phenotypic differences diabetic BB/OK and BB/Mol rats characterized by significantly different frequency and age at onset of diabetes were reciprocally crossed. F1 females of both crosses were backcrossed onto diabetic BB/Mol rats resulting 94 BC1 hybrids which were analyzed for 30 polymorphic microsatellite markers on 14 chromosomes. For the first time it is shown that a diabetes protective locus on chromosome X and a gene around the D10Mit9 locus on chromosome 10 can explain the low frequency (ca. 50%) and the late age at onset of diabetes (ca. 130 days) in the BB/OK rat subline, respectively.
Klöting
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I
Department of Laboratory Animal Science, University of Greifswald, Karlsburg, Germany.
Kovacs
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P
D017668:Age of Onset; D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003922:Diabetes Mellitus, Type 1 / Q000235:genetics*; D005260:Female; D008297:Male; D018895:Microsatellite Repeats; D010641:Phenotype; D051381:Rats; D011913:Rats, Inbred BB; D012727:Sex Characteristics; D014960:X Chromosome