title: Developmentally regulated Xist promoter switch mediates initiation of X inactivation.
abstract: Developmental regulation of the mouse Xist gene at the onset of X chromosome inactivation is mediated by RNA stabilization. Here, we show that alternate promoter usage gives rise to distinct stable and unstable RNA isoforms. Unstable Xist transcript initiates at a novel upstream promoter, whereas stable Xist RNA is transcribed from the previously identified promoter and from a novel downstream promoter. Analysis of cells undergoing X inactivation indicates that a developmentally regulated promoter switch mediates stabilization and accumulation of Xist RNA on the inactive X chromosome.
authors.affiliation: MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom.
authors.forename: C M
authors.initials: CM
authors.lastname: Johnston
authors.forename: T B
authors.initials: TB
authors.lastname: Nesterova
authors.forename: E J
authors.initials: EJ
authors.lastname: Formstone
authors.forename: A E
authors.initials: AE
authors.lastname: Newall
authors.forename: S M
authors.initials: SM
authors.lastname: Duthie
authors.forename: S A
authors.initials: SA
authors.lastname: Sheardown
authors.forename: N
authors.initials: N
authors.lastname: Brockdorff
mesh_terms: D000818:Animals; D002460:Cell Line; D004303:Dosage Compensation, Genetic; D005314:Embryonic and Fetal Development / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D017404:In Situ Hybridization, Fluorescence; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000502:physiology*; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000378:metabolism; D022661:RNA, Untranslated; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D014960:X Chromosome
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D022661:RNA, Untranslated; D014157:Transcription Factors; C077885:XIST non-coding RNA