Identification of a novel human homolog of the Drosophila dlg, P-dlg, specifically expressed in the gland tissues and interacting with p55.
We have identified a novel human homolog of the Drosophila dlg tumor suppressor gene, termed P-dlg, which has been mapped at chromosome 10q23. Unlike other human dlg homologs, P-dlg is expressed in placenta and various gland tissues but not in brain. The P-dlg protein is localized at the plasma membrane and cytoplasm, and it is expressed in the gland epithelial cells in normal prostate tissue but not in prostate cancer cell lines. Furthermore, we identified interaction between P-dlg and p55 palmitoylated membrane protein by yeast two-hybrid screening. These findings suggest that P-dlg forms a complex with p55 at the plasma membrane and plays roles in maintaining the structure of epithelial cells and transmitting extracellular signals to the membrane and cytoskeleton, which may negatively regulate cell proliferation.
Nakamura
H
H
Department of Tumor Genetics and Biology, Kumamoto University School of Medicine, Honjo, Japan.
Sudo
T
T
Tsuiki
H
H
Miyake
H
H
Morisaki
T
T
Sasaki
J
J
Masuko
N
N
Kochi
M
M
Ushio
Y
Y
Saya
H
H
D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D004330:Drosophila / Q000235:genetics; D029721:Drosophila Proteins; D015870:Gene Expression; D016147:Genes, Tumor Suppressor; D051528:Guanylate Kinases; D006801:Humans; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism*; D009928:Organ Specificity; D010446:Peptide Fragments / Q000378:metabolism; D011467:Prostate / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D025521:Tumor Suppressor Proteins
D002352:Carrier Proteins; C114461:DLG5 protein, human; D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D010446:Peptide Fragments; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins; C070350:dlg1 protein, Drosophila; D009703:Nucleoside-Phosphate Kinase; D051528:Guanylate Kinases