Biochemical separations by continuous-bed chromatography.
Innovations in column-packing media for biomolecule purification have progressed from large spherical, porous polysaccharide beads to advanced polymeric supports. Continuous-bed technology is a radical new technology for chromatography based on the polymerization of advanced monomers and ionomers directly in the chromatographic column. The polymer chains form aggregates which coalesce into a dense, homogeneous network of interconnected nodules consisting of microparticles with an average diameter of 3000 A. The voids or channels between the nodules are large enough to permit a high hydrodynamic flow. Due to the high cross-linking of the polymer matrix, the surface of each nodule is nonporous yet the polymeric microparticles provide a very large surface area for high binding capacity. This paper will demonstrate the properties and advantages of using a continuous bed support for high resolution biomolecule separations at high flow-rates without sacrificing capacity.
Tisch
T L
TL
Bio-Rad Laboratories, BioMaterials Division, Hercules, CA 94547, USA. ted_tisch@bio-rad.com
Frost
R
R
Liao
J L
JL
Lam
W K
WK
Remy
A
A
Scheinpflug
E
E
Siebert
C
C
Song
H
H
Stapleton
A
A
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008628:Mercury; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000302:isolation & purification; D009865:Oocytes / Q000737:chemistry; D010047:Ovalbumin / Q000302:isolation & purification; D010316:Particle Size; D011134:Polysaccharides; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D015203:Reproducibility of Results; D014982:Xenopus laevis
D011134:Polysaccharides; D011506:Proteins; D010047:Ovalbumin; D009113:Muramidase; D008628:Mercury