Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae.
Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 background. Disrupting the CTS1 (chitinase) gene also produces pseudohyphal growth in this background, but at a reduced level. Invasion of solid media by haploid and diploid cells is increased in ACE2 disruptions, but the diploids adhere poorly to the agar. Sigma1278b-derived strains, which generally produce pseudohyphae, have about 30-fold lower chitinase activity than other strains.
King
L
L
Department of Biochemistry, University College Dublin, Belfield, Dublin 4, Ireland.
Butler
G
G
D002448:Cell Adhesion / Q000235:genetics; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics; D009154:Mutation; D009687:Nuclear Proteins; D011003:Ploidies; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*
C066778:ACE2 protein, S cerevisiae; D004268:DNA-Binding Proteins; C100940:FLO8 protein, S cerevisaie; D005656:Fungal Proteins; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D002686:Chitin; D002688:Chitinases