Structure-function relationships in the ezrin family and the effect of tumor-associated point mutations in neurofibromatosis 2 protein.
Ezrin, radixin and moesin (ERM proteins) link cell adhesion molecules to the cytoskeleton, modulate cell morphology and cell growth and are involved in Rho-mediated signal transduction. Merlin, the tumor suppressor in neurofibromatosis 2, is a diverged member of the ezrin family, but its function is at least partially similar to the ERM proteins. In the N-domain, the ezrin family belongs to the band 4.1 superfamily. Secondary structure predictions made separately for the ezrin and band 4.1-tyrosine phosphatase families give a similar pattern for the homologous N-domains, indicating that both families have a similar binding site for the integral membrane proteins. The alpha-domain shows a strong coiled-coil prediction, that can be involved in the protein dimerization. The C-terminal actin-binding site in the ERM proteins and the actin-binding helix in the villin headpiece have a common amino acid motif. In merlin, the published tumor-associated single amino acid mutations in the N-domain are located in the conserved sites, and they affect mainly the predicted helices and strands, indicating that these mutations cause the disease primarily by disturbing the protein structure. In the alpha- and C-domains, some of the mutations break the helical structures. Some known mutations are observed at a site potentially interacting with cell adhesion molecules. We will also discuss the implications of the evolutionary information and the actin-binding models in the ezrin family.
Turunen
O
O
Department of Virology, Haartman Institute, University of Helsinki, POB 21, FIN-00014 Helsinki, Finland. Ossi.Turunen@Helsinki.Fi
Sainio
M
M
Jääskeläinen
J
J
Carpén
O
O
Vaheri
A
A
D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D003598:Cytoskeletal Proteins; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics; D009154:Mutation / Q000235:genetics; D016518:Neurofibromatosis 2 / Q000235:genetics*; D025581:Neurofibromin 2; D010750:Phosphoproteins / Q000737:chemistry*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D013329:Structure-Activity Relationship; D000099149:Ezrin
D001798:Blood Proteins; D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D025581:Neurofibromin 2; D010750:Phosphoproteins; D000099149:Ezrin; C071776:radixin