title: Characteristics of four cowpox virus isolates from Norway and Sweden.
abstract: We report the first isolation of cowpox virus from a domestic cat in Norway, and the first confirmed isolation of cowpox virus from a human case in Norway. These two Norwegian cowpox virus isolates, as well as two Swedish human isolates, were partially characterized and compared with each other and with cowpox virus Brighton and vaccinia virus strain Western Reserve. Restriction enzyme analysis of the genomes revealed differences between all six viruses examined, but suggested that the two Norwegian isolates are closely related, as are the two Swedish isolates. Restriction endonuclease digestion of genomic DNA demonstrated that one of the Swedish isolates and the two Norwegian isolates have larger genomes than vaccinia virus strain Western Reserve, but smaller than cowpox Brighton. All four Scandinavian isolates lacked a 72 base-pair region within the A-type inclusion body protein gene which is present in the prototype cowpox virus Brighton.
authors.affiliation: Department of Arctic Veterinary Medicine, Norwegian College of Veterinary Medicine, Tromsø.
authors.forename: M
authors.initials: M
authors.lastname: Tryland
authors.forename: T
authors.initials: T
authors.lastname: Sandvik
authors.forename: H
authors.initials: H
authors.lastname: Hansen
authors.forename: G
authors.initials: G
authors.lastname: Haukenes
authors.forename: L
authors.initials: L
authors.lastname: Holtet
authors.forename: M
authors.initials: M
authors.lastname: Bennett
authors.forename: R
authors.initials: R
authors.lastname: Mehl
authors.forename: U
authors.initials: U
authors.lastname: Moens
authors.forename: O
authors.initials: O
authors.lastname: Olsvik
authors.forename: T
authors.initials: T
authors.lastname: Traavik
mesh_terms: D000293:Adolescent; D000328:Adult; D000482:Allantois / Q000821:virology; D000818:Animals; D015139:Blotting, Southern; D002415:Cats; D002642:Chick Embryo; D002648:Child; D002823:Chorion / Q000821:virology; D015605:Cowpox / Q000453:epidemiology / Q000821:virology*; D015608:Cowpox virus / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D005260:Female; D016679:Genome, Viral; D006801:Humans; D009664:Norway / Q000453:epidemiology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013548:Sweden / Q000453:epidemiology; D013937:Thymidine Kinase / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: C088596:A-type inclusion protein, poxvirus; D014764:Viral Proteins; D013937:Thymidine Kinase