Effect of glycosylated and non-glycosylated prolactin on the proliferation of normal human lymphocytes.
We evaluated the effects of 2.5x10(-10) M or 5x10(-10) M concentrations of human pituitary prolactin (pPRL), human recombinant non-glycosylated (NG-PRL) and glycosylated (GL-PRL) prolactin on the proliferation of normal human lymphocytes with or without coactivation by interleukin-2 (IL-2). None of the PRL forms alone affected the lymphocyte proliferation in a serum-free medium, however, the stimulatory activity of IL-2 was significantly potentiated with all 3 PRL variants. Since the 5x10(-10) M concentrations of individual PRLs exerted the same effects, this result suggests that GL-PRL in primary lymphocyte culture is not a less mitogenic form, if sufficient amounts of IL-2 are available.
Jurcovicová
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Krueger
K S
KS
Rokyta
R
R
D002455:Cell Division / Q000187:drug effects*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008297:Male; D011388:Prolactin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology
D007376:Interleukin-2; D011994:Recombinant Proteins; C043928:prolactin, glycosylated; D011388:Prolactin