title: Antigenic diversity and serotypes of Helicobacter pylori associated with peptic ulcer diseases.
abstract: OBJECTIVE
  Clinical presentation of Helicobacter pylori (H. pylori) infection has marked variation mainly due to the strain diversity and host susceptibility. Although H. pylori is identified as a major risk factor for gastric and duodenal ulcers, the ulcerogenic or pathogenic strain has not been documented yet. The objective of this study was to investigate antigenic types of the ulcerogenic strain of H. pylori.
  
  
  METHODS
  The sera of 64 patients were tested by Western blot using Helicoblot 2.0 for six major anti-H. pylori antibodies, together with CLO test and histological examination of gastric biopsy tissues. Thirty-five, nine and 20 patients had duodenal ulcer, gastric ulcer and chronic active gastritis, respectively. The antigenic types of H. pylori were analyzed in 54 patients with positive H. pylori infection. In this study, H. pylori was divided into four serotypes according to the presence and absence of CagA and VagA: type I; CagA (+) and VacA(+), type Ia: CagA (+) and VacA(-), type Ib: CagA(-) and VacA(+), and type II: CagA(-) and VacA(-).
  
  
  RESULTS
  There was no difference in the number of bands for six antigens: 3.2 +/- 1.4, 3.0 +/- 1.2 and 3.1 +/- 1.4 in 35 duodenal ulcer, 7 gastric ulcer and 12 chronic gastritis, respectively. The band with 119 kDa was 90.7%, which was the most common band with the order of 35, 30, 26.5, 89 and 19.5 kDa. Type I, la and Ib were positive in 22.2, 42.6 and 27.8%, respectively, which were significantly higher than type II (p < 0.05). There was no difference in the positive rates of four urease subtypes between the four serotypes.
authors.affiliation: Department of Internal Medicine, College of Medicine, Chungbuk National University, Cheongju, Korea.
authors.forename: S M
authors.initials: SM
authors.lastname: Park
authors.forename: S I
authors.initials: SI
authors.lastname: Hong
authors.forename: H Y
authors.initials: HY
authors.lastname: Jung
authors.forename: S K
authors.initials: SK
authors.lastname: Yang
authors.forename: H R
authors.initials: HR
authors.lastname: Kim
authors.forename: Y I
authors.initials: YI
authors.lastname: Min
authors.forename: W S
authors.initials: WS
authors.lastname: Hong
mesh_terms: D000328:Adult; D000368:Aged; D000942:Antigens, Bacterial / Q000032:analysis / Q000145:classification*; D015153:Blotting, Western; D002908:Chronic Disease; D004381:Duodenal Ulcer / Q000276:immunology / Q000382:microbiology* / Q000473:pathology; D005753:Gastric Mucosa / Q000382:microbiology / Q000473:pathology; D005756:Gastritis / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000276:immunology*; D016480:Helicobacter pylori / Q000276:immunology*; D006801:Humans; D008875:Middle Aged; D012703:Serotyping; D013276:Stomach Ulcer / Q000276:immunology / Q000382:microbiology* / Q000473:pathology
chemical_list: D000942:Antigens, Bacterial