Simple purification procedure for human prostatic kallikrein hK2 in its active form.
Kallikrein hK2 is a new potential marker of prostate cancer. It is the last member of the human kallikrein gene family to be isolated. We propose a simple purification procedure permitting us to obtain the active form of hK2 starting from human seminal plasma and using commonly available chromatography matrices. In contrast to recently published papers, this procedure is carried out without any immunoaffinity chromatography step and without the need for any antibody to follow the purification. Furthermore, it does not require any recombinant DNA technology nor sophisticated instruments.
Frenette
G
G
Laboratoire de Biorégulation hormonale, Le Centre Hospitalier Universitaire de Québec, Pavillon CHUL, Sainte-Foy, Canada.
Tremblay
R R
RR
Dubé
J Y
JY
D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D015153:Blotting, Western; D002847:Chromatography, Agarose; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007610:Kallikreins / Q000302:isolation & purification*; D008297:Male; D011471:Prostatic Neoplasms / Q000175:diagnosis; D012661:Semen / Q000737:chemistry; D020840:Tissue Kallikreins
D014408:Biomarkers, Tumor; D007610:Kallikreins; D020840:Tissue Kallikreins