Two novel adenovirus vector systems permitting regulated protein expression in gene transfer experiments.
Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766-1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180-8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.
Molin
M
M
Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, S-751 23 Uppsala, Sweden.
Shoshan
M C
MC
Ohman-Forslund
K
K
Linder
S
S
Akusjärvi
G
G
D016475:3T3 Cells; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D019556:COS Cells; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000187:drug effects*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D051379:Mice; D015735:Mifepristone / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology
D006727:Hormone Antagonists; D015735:Mifepristone; D015500:Chloramphenicol O-Acetyltransferase; D013752:Tetracycline