title: Localization of a G protein Gi2 in the cilia of rat ependyma, oviduct and trachea.
abstract: In previous studies, the localization of a pertussis toxin-sensitive G protein was demonstrated in ependymal cilia, but the identification of the subtype of G protein was inconsistent. To clarify this issue, we studied the localization of Goalpha, Gi1alpha, Gi3alpha and Gi2alpha in the ciliated ependymal cells and in the cilia of some other tissues of rats using specific antibodies. The cilia of the ependymal cells that line the ventricular cavity of the brain were intensely immunoreactive for Gi2alpha, but not for Goalpha, Gi1alpha or Gi3alpha. Immunoblot analysis demonstrated higher levels of Gi2alpha in the ependymal cilia-rich pellet than in the motor area of the parietal cortex. At the ultrastructural level, the immunoreactivity specific for Gi2alpha was found predominantly in the cilia, but rarely in the microvilli or the basal bodies of ependymal cells. In cross-sections, the immunoreactivity specific for Gi2alpha was observed only in cell membranes, in particular, in the inner electron-dense leaflet of the trilaminar structure. In addition to that in the ependymal cilia, such specific localization of Gi2alpha was observed in the motile cilia in other tissues, including the oviduct and trachea. By contrast, the stereocilia in the ductus deferens were not immunopositive for Gi2alpha. These findings suggest that Gi2 might play an important role in the signal transduction in ciliary membrane-associated function(s) of the ependymal cells, oviduct and trachea.
authors.affiliation: Department of Anatomy, Mie University School of Medicine, Tsu, Japan. sinohara@doc.medic.mie-u.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Shinohara
authors.forename: T
authors.initials: T
authors.lastname: Asano
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: T
authors.initials: T
authors.lastname: Kameshima
authors.forename: R
authors.initials: R
authors.lastname: Semba
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002923:Cilia / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004805:Ependyma / Q000033:anatomy & histology / Q000378:metabolism*; D005187:Fallopian Tubes / Q000033:anatomy & histology / Q000378:metabolism*; D005260:Female; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism*; D007150:Immunohistochemistry; D008297:Male; D016253:Microscopy, Immunoelectron; D010296:Parietal Lobe / Q000033:anatomy & histology / Q000502:physiology; D016613:Plastic Embedding; D051381:Rats; D017208:Rats, Wistar; D014132:Trachea / Q000033:anatomy & histology / Q000378:metabolism*
chemical_list: D019206:GTP-Binding Protein alpha Subunits, Gi-Go