title: Characterization of a macaque anti-Rh17-like monoclonal antibody.
abstract: In order to produce macaque monoclonal antibodies (mAbs) against human red blood cell (RBC) antigens, macaques were immunized with human and gorilla RBCs and their spleen lymphocytes were fused with man-mouse heteromyeloma cells. One macaque-mouse heterohybridoma produced a macaque IgGx (Cvn2-4D5) which agglutinated all human RBCs but not rare human variants Dc-,D-, and Rhnull. Thus, Cyn2-4D5 exhibited RH17-like reactivity. The specificity of Cyn2-4D5 for RHCE-encoded polypeptides was confirmed by specific immunoprecipitation of RhcE and RhCe polypeptides from human RBCs and the absence of immunoprecipitation of the RhD polypeptides extracted from D-RBCs. This study demonstrates that it is possible to produce macaque mAbs against human RBC blood group antigens.
authors.affiliation: Laboratoire d'Immunogénétique Moléculaire, Université Paul-Sabatier, Hôpital Purpan, Toulouse, France.
authors.forename: A
authors.initials: A
authors.lastname: Blancher
authors.forename: F
authors.initials: F
authors.lastname: Roubinet
authors.forename: M E
authors.initials: ME
authors.lastname: Reid
authors.forename: W W
authors.initials: WW
authors.lastname: Socha
authors.forename: P
authors.initials: P
authors.lastname: Bailly
authors.forename: P
authors.initials: P
authors.lastname: Bénard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000918:Antibody Specificity; D004912:Erythrocytes / Q000276:immunology; D006071:Gorilla gorilla / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008252:Macaca fascicularis / Q000276:immunology*; D051379:Mice; D011148:Pongo pygmaeus / Q000276:immunology; D012204:Rh-Hr Blood-Group System / Q000276:immunology*; D013045:Species Specificity; D013154:Spleen / Q000166:cytology
chemical_list: D000911:Antibodies, Monoclonal; D012204:Rh-Hr Blood-Group System