JC9813-A putative novel human papillomavirus identified by PCR-DS.
Papillomaviruses consist of more than 130 viral types described so far. Most of them are human papillomaviruses (HPV) of supergroup A, demonstrating ano-genital tropism and characterized as etiological agents for benign and malignant cervical lesions in women. A PCR-direct sequencing (PCR-DS) approach with P-33 labeled dideoxynucleotides was used to detect and type human papillomaviruses in cervical biopsies. One novel sequence was identified in a LSIL (low-grade squamous intraepithelial lesions) specimen from an HIV-positive English Canadian patient. The structure of the viral gene L1 was determined, yielding a putative novel HPV type of supergroup A (clade A8) named JC9813.
Feoli-Fonseca
J C
JC
Hôpital Ste-Justine, Université de Montréal, Canada.
Oligny
L L
LL
Filion
M
M
Simard
P
P
Russo
P A
PA
Yotov
W V
WV
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D001706:Biopsy; D036022:Capsid Proteins; D004279:DNA, Viral; D005260:Female; D015658:HIV Infections / Q000150:complications; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014412:Tumor Virus Infections / Q000150:complications / Q000473:pathology
D036022:Capsid Proteins; D004279:DNA, Viral; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral