title: Morphological and immunohistochemical aspects of the biological seal in Klöckner's dental implants: study of 15 cases.
abstract: The morphological and immunohistochemical aspects of the peri-implanted pathological soft tissue of 21 Klöckner implants are assessed, corresponding to 15 patients and three gingival samples from three cadavers. For the collagen and vascular walls assessment, the Picro-Sirio technique is used with normal microscopy as well as with polarized light, evaluating the decrease of collagen fibers in relation to the grade of inflammation. In cases when there is a plasmocyte inflammatory prevalence, antibodies are used contrasted with Kappa and Lambda light chains, observing in all cases a polyclonal plasmocytosis (reactive). The Ag. Ki-67 is used to mark cell proliferation that shows a normal or enlarged activity that can reach the infiltrated cells of the lamina propria. Epitheliotropism or a lymphoepithelial lesion is analyzed in relation to the inflammation, observing the tendency to be larger in K-type implants with a completely-submerged technique. The lesion that is usually observed is the periimplanted mucositis of the chronic or mixed type. The regenerated epithelium has a slight thickness with congestive and dilated vessels. In one case (as anecdotal value), there is the presence of coilocytes compatible with infection by HPV (Human Papilloma Virus).
authors.affiliation: Valle de Hebron Hospitals, Autonomous University, Barcelona. Calvo@pulso.com
authors.forename: M A
authors.initials: MA
authors.lastname: Calvo Mateo
authors.forename: M C
authors.initials: MC
authors.lastname: Ruiz Marcellan
authors.forename: M V
authors.initials: MV
authors.lastname: Bañares Agustin
authors.forename: A
authors.initials: A
authors.lastname: Padros Fradrera
authors.forename: E
authors.initials: E
authors.lastname: Sada Moreno
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003238:Connective Tissue / Q000276:immunology / Q000473:pathology; D015921:Dental Implants / Q000009:adverse effects; D004848:Epithelium / Q000276:immunology / Q000473:pathology; D005881:Gingiva / Q000276:immunology / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D008875:Middle Aged; D009061:Mouth Mucosa / Q000473:pathology; D010518:Periodontitis / Q000209:etiology* / Q000276:immunology / Q000473:pathology; D014025:Titanium
chemical_list: D015921:Dental Implants; D014025:Titanium