title: Simultaneous determination of cyanide and thiocyanate in blood by ion chromatography with fluorescence and ultraviolet detection.
abstract: An ion chromatographic method for the simultaneous determination of cyanide and thiocyanate in blood has been developed. After extraction by adding water and methanol to blood, cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorometry and the underivatized thiocyanate was detected by ultraviolet absorption. The detection limits were 3.8 pmol ml(-1) for cyanide and 86 pmol ml(-1) for thiocyanate, and the recoveries from blood were ca. 83% and ca. 100%, respectively. The proposed method was successfully applied to the analysis of both anions in blood from smokers, non-smokers and fire victims.
authors.affiliation: Forensic Science Laboratory, Ishikawa Pref. Police Headquarters, Hirosaka, Kanazawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Chinaka
authors.forename: N
authors.initials: N
authors.lastname: Takayama
authors.forename: Y
authors.initials: Y
authors.lastname: Michigami
authors.forename: K
authors.initials: K
authors.lastname: Ueda
mesh_terms: D002852:Chromatography, Ion Exchange; D003486:Cyanides / Q000097:blood* / Q000506:poisoning; D005390:Fires; D006801:Humans; D007202:Indicators and Reagents; D009281:Naphthalenes; D011190:Potassium Cyanide / Q000506:poisoning; D012680:Sensitivity and Specificity; D012907:Smoking / Q000097:blood; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013405:Suicide; D013654:Taurine; D013861:Thiocyanates / Q000097:blood*
chemical_list: D003486:Cyanides; D007202:Indicators and Reagents; D009281:Naphthalenes; D013861:Thiocyanates; D013654:Taurine; C027717:2,3-naphthalenedicarboxaldehyde; D011190:Potassium Cyanide