Influence of naproxen on the healing of open excision wound in rats.
Excision wounds were made on the paravertebral area on the dorsal side of rat under light ether anaesthesia. The wound was a full thickness wound with removal of the skin upto the fascia. Animals were fed the drug naproxen in a gum acacia suspension with low, median and high doses based on the LD50 value of the drug. The animals were dosed upto the time eschar separated and no raw wound was left. Healing was assessed by planimetric measurement of the wound, periodically, thereby following wound closure by contraction. Granulation tissue formation and wound strength was assessed by using the granuloma model. Epithelialization was also determined. The cellular activity was assessed histologically and the biochemical indices on day of wounding and after the complete healing of the wound. Granulation tissue was used in assessing tensile strength and contraction. Histological sections of the wound on different days were assessed for connective tissue formation by the Mallory-Heidenhan stain. The closure of wounds were represented as percent contraction of original wound size and epithelialization as complete formation of epithelial layer with no rawness of the wound. The scar shape was noted and measured. The results of this study will be discussed.
Margaret
I
I
Biological Sciences Division, Indian Institute of Chemical Technology, Hyderabad, India.
Jamil
K
K
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D003094:Collagen / Q000032:analysis; D005260:Female; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D006909:Hydroxyproline / Q000032:analysis; D008297:Male; D009288:Naproxen / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000187:drug effects*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D009288:Naproxen; D003094:Collagen; D006909:Hydroxyproline