Effect of L-carnitine and palmitoyl-L-carnitine on erythroid colony formation in fetal mouse liver cell culture.
The administration of L-carnitine to patients undergoing hemodialysis increases hematocrit and improves the response to recombinant human erythropoietin (rhEPO). This suggests a contribution by carnitine to erythrocyte membrane function or erythropoiesis. We investigated the effect of L-carnitine and palmitoyl-L-carnitine on erythropoiesis by assessing erythroid colony formation in in vitro fetal mouse liver cell cultures. L-Carnitine or palmitoyl-L-carnitine was added with rhEPO to fetal mouse liver cell cultures. Doses of L-carnitine of up to 200 micromol/l to the culture had no effect on colony formation. In contrast, the addition of above 12.5 micromol/l palmitoyl-L-carnitine into the culture increased colony formation significantly. These results suggest that long-chain acyl carnitine may have an effect on erythropoiesis.
Matsumura
M
M
Second Department of Internal Medicine, School of Medicine, Kanazawa University, Kanazawa, Japan.
Hatakeyama
S
S
Koni
I
I
Mabuchi
H
H
D000818:Animals; D002331:Carnitine / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells / Q000166:cytology / Q000187:drug effects*; D004920:Erythropoiesis / Q000187:drug effects / Q000502:physiology; D004921:Erythropoietin / Q000494:pharmacology; D005333:Fetus; D006801:Humans; D008099:Liver / Q000166:cytology; D051379:Mice; D008813:Mice, Inbred ICR; D010172:Palmitoylcarnitine / Q000494:pharmacology*; D011994:Recombinant Proteins
D011994:Recombinant Proteins; D004921:Erythropoietin; D010172:Palmitoylcarnitine; D002331:Carnitine