[Gestational diabetes--a model trial. The effect of alloxan diabetes on the spectrum of fatty acids in the blood, liver and brain in laboratory rats].
In alloxan-induced diabetic rats (Wistar, females, age 3-4 months of postnatal life) the large spectrum of fatty acids in blood serum, brain cortex, medulla oblongata and liver was studied. The fatty acids, using gas chromatography, were detected as methyl esters and the methods were published previously (Smídová and al. 1994). Alloxan (Merck) administered i.p., 140 mg/kg body weight, caused immediately elevation (three times) of blood sugar levels. On the 13th day the rats were killed. The results are as follows: a) In blood serum alloxan diabetes of cca two weeks duration caused a significantly increased participation of saturated FA and decreased participation of both polyunsaturated FA (n-3 and n-6). b) In brain cortex no differences between controls and diabetic rats in the indicated groups of FA were found. c) In the medulla oblongata an increased participation of polyunsaturated fatty acids n-6 was established. d) In hepatic tissue the increased participation of saturated FA as well as a decreased participation of FA n-6 was described. Analysing the main groups of FA we found especially in n-3 and n-6 FA several significant changes in single FA (a smaller pool of arachidonic acid in blood serum as well as in liver, decreased participation of docosahexaenoic acid in the brain cortex, etc.). The purpose and possible consequences of such changes are discussed.
Mourek
J
J
Fyziologický ústav 1. LF UK, Praha.
Smídová
L
L
Slapetová
V
V
Krejcí
V
V
Plavka
R
R
D000496:Alloxan; D000818:Animals; D001921:Brain / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D016640:Diabetes, Gestational / Q000378:metabolism*; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D000496:Alloxan