title: A Ser315Thr substitution in KatG is predominant in genetically heterogeneous multidrug-resistant Mycobacterium tuberculosis isolates originating from the St. Petersburg area in Russia.
abstract: Parts of katG and rpoB from 27 Russian Mycobacterium tuberculosis isolates were sequenced to detect mutations causing resistance to isoniazid (INH) and rifampin (RMP), respectively. All 24 INH-resistant isolates had a mutated katG, and 22 of them (91.7%) carried a mutation coding for a Ser315Thr shift. An rpoB mutation was noted for each of the 21 RMP-resistant isolates, with Ser531Leu being the most prevalent change encoded. Only two isolates had identical IS6110 fingerprints.
authors.affiliation: Mycobacterial Reference Laboratory, National Public Health Institute, Kiinamyllynkatu 13, 20520 Turku, Finland. harmar@utu.fi
authors.forename: H J
authors.initials: HJ
authors.lastname: Marttila
authors.forename: H
authors.initials: H
authors.lastname: Soini
authors.forename: E
authors.initials: E
authors.lastname: Eerola
authors.forename: E
authors.initials: E
authors.lastname: Vyshnevskaya
authors.forename: B I
authors.initials: BI
authors.lastname: Vyshnevskiy
authors.forename: T F
authors.initials: TF
authors.lastname: Otten
authors.forename: A V
authors.initials: AV
authors.lastname: Vasilyef
authors.forename: M K
authors.initials: MK
authors.lastname: Viljanen
mesh_terms: D001426:Bacterial Proteins; D018432:Drug Resistance, Multiple; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D009154:Mutation; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics; D010544:Peroxidases / Q000235:genetics*; D012293:Rifampin / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D010544:Peroxidases; C076136:catalase HPI; D007538:Isoniazid; D012293:Rifampin