title: Presence of dioxins in human follicular fluid: their possible stage-specific action on the development of preimplantation mouse embryos.
abstract: Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations between 0.5 and 100 pM and evaluated at 24-h intervals for their development to the eight-cell and blastocyst stages. The percentage of eight-cell embryos exposed to TCDD at 1, 2, and 5 pM concentrations was significantly lower than that of controls. However, blastocyst formation of the surviving eight-cell embryos was accelerated, with the number of cells in the blastocysts increased in a dose-dependent manner. Findings suggest that PCDDs and PCDFs may be present in human reproductive fluid and may exert some stage-specific effects on early embryonic development.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan. osamut-tky@umin.ac.jp
authors.forename: O
authors.initials: O
authors.lastname: Tsutsumi
authors.forename: H
authors.initials: H
authors.lastname: Uechi
authors.forename: H
authors.initials: H
authors.lastname: Sone
authors.forename: J
authors.initials: J
authors.lastname: Yonemoto
authors.forename: Y
authors.initials: Y
authors.lastname: Takai
authors.forename: M
authors.initials: M
authors.lastname: Momoeda
authors.forename: C
authors.initials: C
authors.lastname: Tohyama
authors.forename: S
authors.initials: S
authors.lastname: Hashimoto
authors.forename: M
authors.initials: M
authors.lastname: Morita
authors.forename: Y
authors.initials: Y
authors.lastname: Taketani
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D004147:Dioxins / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D051379:Mice
chemical_list: D004147:Dioxins