title: Estrogenic xenobiotics affect the intracellular activation signal in mitogen-induced human peripheral blood lymphocytes: immunotoxicological impact.
abstract: The present study was an attempt to elucidate the effect of estrogenic xenobiotics on the proliferation of mitogen-stimulated human peripheral blood lymphocyte (PBL). Our findings follow: (a) the proliferation of PBL in response to phytohemagglutinin (PHA) was mediated by protein kinase C activity, but estrogenic xenobiotics had a strong inhibitory effect on protein kinase C activity of PHA-stimulated PBL; (b) cytoplasmic extracts from PHA-stimulated PBL greatly activated DNA replication, but estrogenic xenobiotics had a strong inhibitory effect on these activities. The results suggest that the cytoplasmic signal-generating system in mitogen-treated PBL is inhibited by estrogenic xenobiotics, and that the defect occurs at all stages in the sequence of events leading to DNA synthesis and cell proliferation.
authors.affiliation: Department of Anatomy, Tokai University School of Medicine, Isehara, Kanagawa, Japan. sakbel@ibm.net
authors.forename: K
authors.initials: K
authors.lastname: Sakabe
authors.forename: M
authors.initials: M
authors.lastname: Okuma
authors.forename: M
authors.initials: M
authors.lastname: Kazuno
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Yoshida
authors.forename: H
authors.initials: H
authors.lastname: Furuya
authors.forename: F
authors.initials: F
authors.lastname: Kayama
authors.forename: Y
authors.initials: Y
authors.lastname: Suwa
authors.forename: W
authors.initials: W
authors.lastname: Fujii
authors.forename: K L
authors.initials: KL
authors.lastname: Fresa
mesh_terms: D000328:Adult; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology; D045166:Estradiol Congeners / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D015262:Xenobiotics / Q000494:pharmacology*
chemical_list: D045166:Estradiol Congeners; D015262:Xenobiotics; D004958:Estradiol; D011493:Protein Kinase C