Serotaxonomic analysis of glycolipids from Mycobacterium chelonae-M. fortuitum complex and bovine farcy strains.
The antigenicity and cross-reactivity of glycolipids from strains of bovine farcy and the Mycobacterium chelonae-M. fortuitum complex were analyzed using the ELISA technique. Purified alkali-stable glycopeptidolipids (GPLs) with a characteristic dimethylrhamnosyl sugar unit extracted from M. abscessus, M. chelonae, M. peregrinum and M. senegalense, gave very strong reactions with sera against members of the same four species. Particularly strong cross-reactions were evident between M. peregrinum and M. senegalense. These GPLs reacted more weakly with antisera against the other mycobacteria tested, though clear reactions were noticed with M. farcinogenes and M. fortuitum and also with M. bovis BCG, M. phlei, and M. tuberculosis strains. Alkali-labile diacyl trehalose (DAT) and triacyl trehalose (TAT) from M. fortuitum reacted with homologous sera, and with that against M. tuberculosis. Traces of uncharacterized acyl trehaloses isolated from two strains of M. farcinogenes gave comparatively weak reactions. Mycobacteria labeled M. farcinogenes and M. senegalense produced glucosylated trehalose-based glycolipids (GTs) and the studies showed that the major type was antigenic. These glycolipids cross-reacted strongly with M. senegalense NCTC 4524 but not with the type strain of M. senegalense. On the basis of the chemical patterns and the antigenicity of the GPLs it is evident that M. peregrinum and M. senegalense are particularly closely related and these species show a very close affinity to M. abscessus-M. chelonae.
Hamid
M E
ME
Department of Medicine, Pharmacology and Toxicology, Faculty of Veterinary Science, University of Khartoum, Sudan.
Ridell
M
M
Minnikin
D E
DE
Goodfellow
M
M
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000954:Antigens, Surface / Q000276:immunology; D015373:Bacterial Typing Techniques; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006017:Glycolipids / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000276:immunology; D009164:Mycobacterium Infections / Q000382:microbiology / Q000662:veterinary*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology / Q000662:veterinary; D016926:Mycobacterium chelonae / Q000145:classification* / Q000276:immunology; D019907:Mycobacterium fortuitum / Q000145:classification* / Q000276:immunology; D012703:Serotyping
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D006017:Glycolipids