[Reverting effect of cysteine on the suppression by glucose or dexamethasone of anti-Candida activity of human neutrophils].
Amino acid mixture prescribed for an hyperalimentation solution (PN-twin) diminishes suppression of anti-Candida activity of neutrophils as reported previously (Tansho, T, et al. J. Jpn. Assoc. Infect. Dis. 70: 463-469). The aim of this study was to identify the active principle in the amino acid mixture and to examine its action mechanism. Amino acid mixture (PN-twin) containing 23 amino acids neutralized the suppression of anti-Candida activity of human neutrophils by 1.0% of glucose. These amino acids were divided to several groups by their structure and effects of the groups on the suppressed anti-Candida activity neutrophils were examined. In all groups tested, amino acids containing cystein and methionine clearly neutralized the suppression, especially cysteine at the concentration more than 20 micrograms/ml significantly recovered the anti-Candida activity of neutrophils which was suppressed in the presence of 1% glucose or 10(-6) M dexamethasone. Correspondingly, cysteine augmented production of lactoferrin by stimulated neutrophils; which functions as a major effector molecule in growth inhibition of Candida by neutrophils. These results suggest that cysteine in alimentation solution augments anti-Candida defense mechanisms through recovery of neutrophil function.
Tansho
T
T
Department of Surgery II, Teikyo University School of Medicine.
D000596:Amino Acids / Q000494:pharmacology; D002175:Candida / Q000276:immunology*; D003545:Cysteine / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology*
D000596:Amino Acids; D003907:Dexamethasone; D005947:Glucose; D003545:Cysteine