title: Fatty acid omega and (omega-1)-oxidation within intrasplenically transplanted fetal hepatocytes.
abstract: BACKGROUND
  The expression and enzymatic activity of the cytochrome P450 LAomega within intrasplenically transplanted hepatocytes was investigated.
  
  
  METHODS
  Fetal hepatocytes were harvested from spontaneously hypertensive rats and transplanted into recipient adult spontaneously hypertensive rat spleens.
  
  
  RESULTS
  Microscopic examination revealed masses of hepatocytes in the red pulp. Immunochemical studies detected cytochrome P450 LAomega in transplanted hepatocytes by 6 and 10 weeks after transplantation. Cytochrome P450 LAomega mRNA accumulates at 6 weeks after transplantation. Cytochrome P450-arachidonic acid omega/omega-1 hydroxylase activity (formation of 20/19-hydroxyeicosatetraenoic acid) was detected at 10 weeks after transplantation.
  
  
  CONCLUSIONS
  These results demonstrated that fetal hepatocytes grow in the spleen and function similarly to adult hepatocytes.
authors.affiliation: Department of Gastrointestinal Surgery, New York Medical College, Valhalla 10595, USA. kato@asahikawa-med.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Kato
authors.forename: K
authors.initials: K
authors.lastname: Onodera
authors.forename: H
authors.initials: H
authors.lastname: Sakata
authors.forename: M
authors.initials: M
authors.lastname: Imai
authors.forename: J
authors.initials: J
authors.lastname: Kato
authors.forename: W J
authors.initials: WJ
authors.lastname: Hodgson
authors.forename: N G
authors.initials: NG
authors.lastname: Abraham
authors.forename: S
authors.initials: S
authors.lastname: Kasai
authors.forename: M
authors.initials: M
authors.lastname: Mito
mesh_terms: D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D017690:Cell Transplantation; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis; D005227:Fatty Acids / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000166:cytology* / Q000196:embryology / Q000378:metabolism*; D010084:Oxidation-Reduction; D051381:Rats; D011918:Rats, Inbred SHR; D013154:Spleen
chemical_list: D005227:Fatty Acids; D016718:Arachidonic Acid; D003577:Cytochrome P-450 Enzyme System