Ca2+- and Mg2+-modulated lipolysis in neonatal rat brain slices observed by one- and two-dimensional NMR.
Superfused cortical brain slices from neonatal rats demonstrated large increases in levels of NMR-detectable lipids after sample preparation and perfusion with standard artificial CSF. These increases were reduced by an average of 58% by perfusion with buffer with low (no added) Ca2+ or by perfusion in Ca2+-free buffer. Perfusion with buffer with elevated MgSO4 (10 mmol/L) reduced the lipid changes by 47%. A reduction of 88% was observed in samples perfused in buffer with both low Ca2+ and high Mg2+, suggesting a role for Mg2+ in reducing lipolysis distinct from its known ability to block Ca2+ influx.
Gasparovic
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C
Department of Neurosciences and Center for Non-Invasive Diagnosis, University of New Mexico School of Medicine, Albuquerque 87131, USA.
Berghmans
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K
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism*; D066298:In Vitro Techniques; D008066:Lipolysis; D008274:Magnesium / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010477:Perfusion; D010759:Phosphorus Isotopes; D011522:Protons; D051381:Rats; D017207:Rats, Sprague-Dawley
D010759:Phosphorus Isotopes; D011522:Protons; D008274:Magnesium; D002118:Calcium