Polymerase chain reaction for rapid detection of Campylobacter jejuni in artificially contaminated foods.
Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced and raw poultry products tested. All vegetables tested were compatible with the PCR assay. Cantaloupe, kiwi and pineapple tested positive while strawberries, watermelon, grapes and apples tested negative. By using a nested PCR assay that yields a single band at 122 bp, positive results were obtained with watermelon and grapes while the apple and strawberries continued to give a negative reaction. These rapid and specific assays for Camp. jejuni are compatible with most foods in insuring the safety of the food product.
Winters
D K
DK
University of Arkansas, Department of Poultry Science, Fayetteville, AR 72701. dwinters@comp.uark.edu
O'Leary
A E
AE
Slavik
M F
MF
D000818:Animals; D016123:Campylobacter jejuni / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000032:analysis; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D008460:Meat / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D014675:Vegetables / Q000382:microbiology
D004269:DNA, Bacterial