Molecular characterization of two genes encoding betaine aldehyde dehydrogenase from amaranth. Expression in leaves under short-term exposure to osmotic stress or abscisic acid.
A genomic clone (ahybadh4) and a cDNA (ahybadh17) both encoding betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8) were isolated from the plant Amaranthus hypochondriacus L. The ahybadh4 gene extends 9 kilobases (kb) containing 15 exons with an open reading frame (ORF) of 501 amino acids (aa), a 1.3kb 5' untranslated region (UTR) and a 3' UTR of 0.3kb. The ahybadh17 cDNA encodes a BADH isoform of 500aa which contains 10aa substitutions with respect to AHYBADH4. Both encoded proteins share 98% identity at the amino acid level. Comparison of amaranth BADHs with other reported sequences showed high similarity. Analysis of ahybadh17 expression in amaranth leaves showed that mRNA and BADH protein are present in non-treated amaranth leaves and both transiently increased under short-term exposure to abscisic acid (ABA) and osmotic stress treatments.
Legaria
J
J
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, UNAM, Av. Universidad 2001, Col. Chamilpa, 62210, Cuernavaca Mor., Mexico.
Rajsbaum
R
R
Muñoz-Clares
R A
RA
Villegas-Sepúlveda
N
N
Simpson
J
J
Iturriaga
G
G
D000040:Abscisic Acid / Q000494:pharmacology; D000445:Aldehyde Oxidoreductases / Q000235:genetics*; D050728:Betaine-Aldehyde Dehydrogenase; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015870:Gene Expression; D017343:Genes, Plant; D010944:Plants / Q000201:enzymology* / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D000040:Abscisic Acid; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase