Identification of the 150-kDa surface antigen of Entamoeba histolytica as a galactose- and N-acetyl-D-galactosamine-inhibitable lectin.
A monoclonal antibody that reacts with a 150-kDa protein of Entamoeba histolytica on Western immunoblotting under nonreducing conditions inhibits the adherence and cytotoxicity of the ameba to mammalian cells in vitro. Affinity purification of solubilized trophozoites using the monoclonal antibody and electrophoresis yielded three glycoproteins with molecular masses of 150, 170, and 260 kDa, suggesting the existence of either a common epitope or the close association of these proteins. The 260-kDa fraction was identified as the well-known galactose (Gal)- and N-acetyl-D-galactosamine (GalNAc)-inhibitable lectin. The 150- and 170-kDa fractions seemed to exist as part of a 380-kDa native protein with an isoelectric point of pH 6.9. The N-terminal amino acid sequence of the 150-kDa protein was unique, indicating that the protein was not a degraded product of the 260-kDa lectin. By gel filtration, the 260-kDa lectin and the 150/170-kDa protein could be separated. When Chinese hamster ovary cells were pretreated with the fraction consisting of the 150/170-kDa protein the adherence of trophozoites to Chinese hamster ovary cells was competitively inhibited to a level equivalent to that observed for the 260-kDa lectin. The inhibitory effect was lost in the presence of Gal and GalNAc but was not influenced by the presence of glucose. These results demonstrate that the 150/170-kDa protein is a Gal/GalNAc-inhibitable lectin. The existence of a sugar-binding domain in the protein was confirmed by Gal-affinity chromatography.
Cheng
X J
XJ
Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Tsukamoto
H
H
Kaneda
Y
Y
Tachibana
H
H
D000116:Acetylgalactosamine / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D000954:Antigens, Surface / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D015153:Blotting, Western; D016466:CHO Cells; D002448:Cell Adhesion; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000276:immunology* / Q000502:physiology; D005690:Galactose / Q000494:pharmacology; D037102:Lectins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000032:analysis / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology
D000953:Antigens, Protozoan; D000954:Antigens, Surface; D037102:Lectins; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C052452:galactose inhibitable adherence protein, Entamoeba histolytica; D000116:Acetylgalactosamine; D005690:Galactose