title: Developmental changes and functional properties of human memory T cell subpopulations defined by CD60 expression.
abstract: The present study was undertaken to examine developmental changes of T cells expressing CD60 and their functional properties. Three-color immunofluorescence analysis revealed that the CD60 antigen was preferentially expressed on a proportion of memory (CD45RO+) CD4+ T cells, but less on memory CD8+ T cells, while this antigen is undetectable in naive (CD45RO-) T cells. A frequency of memory CD4+ T cells expressing CD60 in the peripheral blood was negligible in newborns and gradually increased with advancing age. CD60+ memory CD4+ T cells showed stronger proliferative responses to PPD and produced higher levels of IL-4 and IL-10 than CD60- ones, whereas production of IL-2 and IFN-gamma was similarly found in both cell subpopulations. In addition, it was shown that efficient helper activity for Ig production by B cells was predominated in CD60+ memory CD4+ T cells. These results suggest that CD60 may be primarily expressed on the functionally differentiated memory effector cells among circulating CD45RO+ CD4+ T cells.
authors.affiliation: Department of Pediatrics, Kanazawa University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Wada
authors.forename: H
authors.initials: H
authors.lastname: Seki
authors.forename: A
authors.initials: A
authors.lastname: Konno
authors.forename: K
authors.initials: K
authors.lastname: Ohta
authors.forename: K
authors.initials: K
authors.lastname: Nunogami
authors.forename: H
authors.initials: H
authors.lastname: Kaneda
authors.forename: Y
authors.initials: Y
authors.lastname: Kasahara
authors.forename: A
authors.initials: A
authors.lastname: Yachie
authors.forename: S
authors.initials: S
authors.lastname: Koizumi
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
authors.lastname: Miyawaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007156:Immunologic Memory; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000032:analysis
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D005732:Gangliosides; D007136:Immunoglobulins; C026226:ganglioside, GD3; D017493:Leukocyte Common Antigens