Development of novel cell surface CD34-targeted recombinant adenoassociated virus vectors for gene therapy.
Recombinant adenoassociated virus (rAAV) type 2 vectors have been used to transduce a wide variety of cell types, including hematopoietic progenitor cells. For in vivo gene transfer, it is desirable to have an rAAV vector that specifically transduces selected target cells. As a first step toward generating an rAAV vector capable of targeting delivery in vivo, we have engineered a chimeric protein combining the AAV capsid protein and the variable region of a single-chain antibody against human CD34 molecules, a cell surface marker for hematopoietic stem/progenitor cells. Inclusion of the chimeric CD34 single-chain antibody-AAV capsid proteins within an rAAV virion significantly increased the preferential infectivity of rAAV for the CD34+ human myoleukemia cell line KG-1, which is normally refractory to rAAV transduction. Antibodies against the single-chain antibody and the CD34 protein blocked this transduction. This chimeric vector represents a significant improvement in the host range of rAAV and the first step toward specific gene delivery by rAAV vectors to cells of choice, in this case, hematopoietic progenitor cells, for the treatment of human disease.
Yang
Q
Q
Immusol, Inc., San Diego, CA 92121, USA.
Mamounas
M
M
Yu
G
G
Kennedy
S
S
Leaker
B
B
Merson
J
J
Wong-Staal
F
F
Yu
M
M
Barber
J R
JR
D000888:Antibodies, Anti-Idiotypic; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D018952:Antigens, CD34 / Q000276:immunology / Q000378:metabolism; D001667:Binding, Competitive; D015415:Biomarkers; D002213:Capsid / Q000235:genetics; D003001:Cloning, Molecular; D000229:Dependovirus / Q000235:genetics* / Q000302:isolation & purification; D018390:Gene Targeting / Q000379:methods*; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D006367:HeLa Cells; D006412:Hematopoietic Stem Cells; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D011993:Recombinant Fusion Proteins; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000096:biosynthesis; D014771:Virion
D000888:Antibodies, Anti-Idiotypic; D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D015415:Biomarkers; D007135:Immunoglobulin Variable Region; D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins