Discrimination of Bordetella parapertussis and Bordetella pertussis organisms from clinical isolates by PCR using biotin-labelled oligonucleotide probes.
A recently developed shared-primer polymerase chain reaction (PCR) was investigated, in an ongoing pertussis surveillance study for discrimination of Bordetella parapertussis and Bordetella pertussis organisms, by using specific biotin-labelled oligonucleotide probes. From a total of 132 samples, 83 were positive by the B. parapertussis specific probe, 33 were positive by the B. pertussis specific probe and 16 samples containing Hemophilus influenzae as a negative control were below threshold by both probes. The shared-primer PCR in combination with specific oligonucleotide probes provides a rapid, sensitive and specific molecular diagnostic tool for future surveillance studies. In addition, it may be used to further investigate whether B parapertussis antigens should be added to acellular pertussis vaccines to protect against B. parapertussis infections.
Müller
F M
FM
Kinderklinik Universitätsklinikum, Aachen, Germany. fmmueller@mail.uniwuerzburg.de
Heininger
U
U
Schnitzler
N
N
Kockelkorn
P
P
Cloot
O
O
Lorenz
C
C
Haase
G
G
D001710:Biotin; D001884:Bordetella / Q000235:genetics* / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000382:microbiology; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000382:microbiology
D015345:Oligonucleotide Probes; D001710:Biotin