title: Biochemical separations by continuous-bed chromatography.
abstract: Innovations in column-packing media for biomolecule purification have progressed from large spherical, porous polysaccharide beads to advanced polymeric supports. Continuous-bed technology is a radical new technology for chromatography based on the polymerization of advanced monomers and ionomers directly in the chromatographic column. The polymer chains form aggregates which coalesce into a dense, homogeneous network of interconnected nodules consisting of microparticles with an average diameter of 3000 A. The voids or channels between the nodules are large enough to permit a high hydrodynamic flow. Due to the high cross-linking of the polymer matrix, the surface of each nodule is nonporous yet the polymeric microparticles provide a very large surface area for high binding capacity. This paper will demonstrate the properties and advantages of using a continuous bed support for high resolution biomolecule separations at high flow-rates without sacrificing capacity.
authors.affiliation: Bio-Rad Laboratories, BioMaterials Division, Hercules, CA 94547, USA. ted_tisch@bio-rad.com
authors.forename: T L
authors.initials: TL
authors.lastname: Tisch
authors.forename: R
authors.initials: R
authors.lastname: Frost
authors.forename: J L
authors.initials: JL
authors.lastname: Liao
authors.forename: W K
authors.initials: WK
authors.lastname: Lam
authors.forename: A
authors.initials: A
authors.lastname: Remy
authors.forename: E
authors.initials: E
authors.lastname: Scheinpflug
authors.forename: C
authors.initials: C
authors.lastname: Siebert
authors.forename: H
authors.initials: H
authors.lastname: Song
authors.forename: A
authors.initials: A
authors.lastname: Stapleton
mesh_terms: D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008628:Mercury; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000302:isolation & purification; D009865:Oocytes / Q000737:chemistry; D010047:Ovalbumin / Q000302:isolation & purification; D010316:Particle Size; D011134:Polysaccharides; D016062:Porosity; D011506:Proteins / Q000302:isolation & purification*; D051381:Rats; D015203:Reproducibility of Results; D014982:Xenopus laevis
chemical_list: D011134:Polysaccharides; D011506:Proteins; D010047:Ovalbumin; D009113:Muramidase; D008628:Mercury