Inhibitory effect of Perilla frutescens and its phenolic constituents on cultured murine mesangial cell proliferation.
The inhibitory effects of Perilla frutescens and its phenolic constituents on cytokine-induced proliferation of murine cultured mesangial cells were investigated. DNA synthesis of mesangial cells stimulated by platelet derived growth factor (PDGF, 10 ng/ml) or tumor necrosis factor (TNF)-alpha (100 U/ml) was inhibited by the extract of P. frutescens (IC50 values, 3.3 and 1.4 micrograms/ml, respectively). The strength of the anti-proliferative activity was nearly equal in various chemotypes of P. frutescens. Caffeic acid, methyl caffeate, rosmarinic acid, and luteolin 7-O-glucuronide-6"-methyl ester were isolated as active constituents from the extract of the typical strain of P. frutescens, and their IC50 values for PDGF-induced mesangial cell proliferation were estimated as 26 microM, 2.6 microM, 1.8 microM, and 4.1 microM, respectively. We also compared the activities of related flavonoids previously isolated from P. frutescens, and luteolin had the highest anti-proliferative activity.
Makino
T
T
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
Ono
T
T
Muso
E
E
Honda
G
G
D000818:Animals; D000077214:Becaplermin; D002109:Caffeic Acids / Q000302:isolation & purification / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002934:Cinnamates / Q000302:isolation & purification / Q000494:pharmacology; D053630:Depsides; D005260:Female; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D005920:Glomerular Mesangium / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D019686:Lamiaceae; D051379:Mice; D008807:Mice, Inbred BALB C; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves; D010946:Plants, Medicinal; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins / Q000494:pharmacology; D013045:Species Specificity; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology / Q000502:physiology; D000097123:Rosmarinic Acid
D000077214:Becaplermin; D002109:Caffeic Acids; D002934:Cinnamates; D053630:Depsides; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D000097123:Rosmarinic Acid