In vitro HIV-1 infection in Macaca nemestrina PBMCs is blocked at a step beyond reverse transcription.
Various stages in the lifecycle of HIV-1 were investigated in Macaca nemestrina and humans in vitro. Early events were analyzed by end point dilution DNA PCR with HIV-1 and SHIV infected PBMCs, while p24 and p27 ELISA assays were used to analyze core antigen production from infected cells. The results demonstrate that a step in the virus life cycle, beyond reverse transcription is blocked for HIV-1 infection in macaque cells.
Kimball
L E
LE
Department of Pathobiology, University of Washington, Seattle 98195, USA.
Bosch
M L
ML
D000818:Animals; D002213:Capsid / Q000096:biosynthesis; D036022:Capsid Proteins; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D015683:Gene Products, gag / Q000096:biosynthesis; D016655:HIV Core Protein p24 / Q000096:biosynthesis; D015497:HIV-1 / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000821:virology*; D008018:Life Cycle Stages; D008254:Macaca nemestrina; D016133:Polymerase Chain Reaction; D015302:Simian Immunodeficiency Virus / Q000254:growth & development / Q000502:physiology*; D014758:Viral Core Proteins / Q000032:analysis / Q000096:biosynthesis; D054301:gag Gene Products, Human Immunodeficiency Virus
D036022:Capsid Proteins; D004279:DNA, Viral; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D016655:HIV Core Protein p24; D014758:Viral Core Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; C074737:p27 gag protein, Human immunodeficiency virus