In vitro production of progesterone and estradiol by rat granulosa cells regulated by cabergoline and prolactin.
Cabergoline (CG), a dopamine agonist, and prolactin (PRL) added in vitro to isolated granulosa cells from immature rats cultured in serum-free medium inhibited FSH-stimulated production of progesterone. The lowest effective concentrations of CG and PRL were 10(-8)M and 1 ng/mL, respectively. In combination, the inhibitory activities were additive. CG at 10(-8) M also suppressed estradiol production by isolated rat granulosa cells; whereas PRL did not. In conclusion, CG inhibits FSH-stimulated progesterone and estradiol production by rat granulosa cells.
Negishi
H
H
Dept. of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
Furuta
I
I
Ushigoe
K
K
Fujimoto
S
S
Koide
S S
SS
D000818:Animals; D000077465:Cabergoline; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D018491:Dopamine Agonists / Q000494:pharmacology; D004873:Ergolines / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
D016895:Culture Media, Serum-Free; D018491:Dopamine Agonists; D004873:Ergolines; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D000077465:Cabergoline