Staphylococcus succinus sp. nov., isolated from Dominican amber.
Two bacterial isolates, designated AMG-D1T and AMG-D2, were recovered from 25-35-million-year-old Dominican amber. AMG-D1T and AMG-D2 biochemically most closely resemble Staphylococcus xylosus; they differ physiologically from other staphylococci. Fatty acid analysis and comparisons with extensive databases were unable to show relatedness to any specific taxon. Moreover, AMG-D1T and AMG-D2 contain tuberculostearic acid and meso-diaminopimelic acid, characteristic of the G + C-rich coryneform bacteria, as opposed to L-lysine characteristic of staphylococci. AMG-D1T and AMG-D2 have a G + C ratio of 35 mol%. Phylogenetic analysis with the 16S rRNA gene indicated that AMG-D1T and AMG-D2 were most closely related to Staphylococcus equorum, S. xylosus, Staphylococcus saprophyticus and other novobiocin-resistant staphylococci. Stringent DNA-DNA hybridization studies with AMG-D1T revealed similarities of 38% with S. equorum, 23% with S. xylosus and 6% with S. saprophyticus. The results indicate that AMG-D1T and AMG-D2 represent a novel species, which was named Staphylococcus succinus sp. nov. The type strain of the new species is AMG-D1 (ATCC 700337).
Lambert
L H
LH
XOMA Corporation, Berkeley, CA 94710, USA.
Cox
T
T
Mitchell
K
K
Rosselló-Mora
R A
RA
Del Cueto
C
C
Dodge
D E
DE
Orkand
P
P
Cano
R J
RJ
D018647:Amber; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D001483:Base Sequence; D002473:Cell Wall; D004269:DNA, Bacterial / Q000032:analysis; D019424:Dominica; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D013210:Staphylococcus / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification / Q000648:ultrastructure
D018647:Amber; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids