title: Protein I/II of oral viridans streptococci increases expression of adhesion molecules on endothelial cells and promotes transendothelial migration of neutrophils in vitro.
abstract: As accumulation of leukocytes in perivascular tissues is a key step in inflammatory disorders, we have analyzed in the present work the up-regulation of expression of adhesion molecules, such as E-selectin, ICAM-1, and VCAM-1, on human endothelial cells, in response to protein I/II, a modulin from Streptococcus mutans OMZ 175. Using cultured human saphenous vein endothelial cells (HSVEC), we demonstrated that protein I/II directly and specifically up-regulated E-selectin, ICAM-1, and VCAM-1 expression. We confirmed also that the up-regulation of adhesion molecules in HSVEC is mediated by lectin activity for NANA- and fucose-containing receptors. The ability of protein I/II to promote the transendothelial migration of neutrophils was then examined. Using Transwell inserts, we found that protein I/II, in promoting the up-regulation of adhesion molecule expression, stimulates neutrophil migration through endothelial cells. These events may play a role in the etiology of inflammatory responses leading to the various pathologies associated with oral viridans streptococci.
authors.affiliation: Faculté de Pharmacie, INSERM Unité 392, Illkirch, France.
authors.forename: A
authors.initials: A
authors.lastname: Vernier-Georgenthum
authors.forename: S
authors.initials: S
authors.lastname: al-Okla
authors.forename: B
authors.initials: B
authors.lastname: Gourieux
authors.forename: J P
authors.initials: JP
authors.lastname: Klein
authors.forename: D
authors.initials: D
authors.lastname: Wachsmann
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D019040:E-Selectin / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D008562:Membrane Glycoproteins; D051379:Mice; D009055:Mouth / Q000382:microbiology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D013295:Streptococcus mutans / Q000502:physiology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D019040:E-Selectin; D008562:Membrane Glycoproteins; C055764:S-layer proteins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1