title: IS481 and IS1002 of Bordetella pertussis create a 6-base-pair duplication upon insertion at a consensus target site.
abstract: The insertion sequence IS481 and its isoform IS1002 have been observed to transpose into the bvgAS locus of Bordetella pertussis, for which the DNA sequence has previously been determined. Upon insertion of IS481 at three different sites and IS1002 at one site, a 6-bp sequence originally present was found at the junction of bvg and insertion sequence DNA. This indicates that, contrary to prior reports, IS481 and IS1002 do create a duplication upon insertion. In this light, examination of these and other examples of IS481 and IS1002 reported in the literature leads to the observation that the 6-bp recognition sequence usually fits the consensus NCTAGN. The near-palindromic nature of this sequence, when directly repeated at the ends of IS481 or IS1002, apparently led to the interpretation that 5 of these base pairs were part of the terminal inverted repeats flanking these elements.
authors.affiliation: Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892, USA. stibitz@helix.nih.gov
authors.forename: S
authors.initials: S
authors.lastname: Stibitz
mesh_terms: D001483:Base Sequence; D001886:Bordetella pertussis / Q000235:genetics*; D004251:DNA Transposable Elements; D006801:Humans; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D004251:DNA Transposable Elements