Tumour necrosis factor beta alleles and hyperinsulinaemia in coronary artery disease.
BACKGROUND
Hyperinsulinaemia and dyslipoproteinaemia are markers and risk factors for coronary artery disease (CAD) and non-insulin-dependent diabetes mellitus (NIDDM). We investigated the influence of a tumour necrosis factor beta (TNF-beta) gene polymorphism on serum parameters related to these metabolic disorders in patients with CAD.


METHODS
A total of 199 patients with CAD and 81 control subjects with angiographically normal coronary arteries were studied. A digestion of amplified DNA with NcoI revealed three fragment patterns: homozygosity for TNF-beta *1 or TNF-beta *2 and heterozygosity (TNF-beta *1/*2).


RESULTS
Patients with CAD who had increased serum insulin or C-peptide (fasting and after glucose load) were predominantly heterozygous for TNF-beta (72% vs. 47%) and less frequently homozygous for TNF-beta *2 (22% vs. 43%, P = 0 x 0.03).


CONCLUSIONS
This study demonstrates an association of TNF-beta alleles with the risk factor hyperinsulinaemia in CAD. Genomic variants of TNF-beta may therefore contribute to the complex susceptibility for the metabolic syndrome in patients with CAD.
Braun
J
J
Klinikum der J. W. Goethe-Universität, Frankfurt am Main, Germany.
März
W
W
Winkelmann
B R
BR
Donner
H
H
Henning Usadel
K
K
Badenhoop
K
K
D000483:Alleles; D002096:C-Peptide / Q000097:blood; D003327:Coronary Disease / Q000097:blood / Q000235:genetics*; D005787:Gene Frequency; D005951:Glucose Tolerance Test; D006349:Heart Valve Diseases / Q000235:genetics; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006946:Hyperinsulinism / Q000097:blood / Q000235:genetics*; D007328:Insulin / Q000097:blood; D008233:Lymphotoxin-alpha / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
D002096:C-Peptide; D007328:Insulin; D008233:Lymphotoxin-alpha