Further purification and structural analysis of calcium spirulan from Spirulina platensis.
An antiviral sulfated polysaccharide, calcium spirulan (Ca-SP), isolated from Spirulina platensis, was subjected to further purification. Ca-SP was found to be composed of rhamnose, 3-O-methylrhamnose (acofriose), 2,3-di-O-methylrhamnose, 3-O-methylxylose, uronic acids, and sulfate. The backbone of Ca-SP consisted of 1,3-linked rhamnose and 1,2-linked 3-O-methylrhamnose units with some sulfate substitution at the 4-position. The polymer was terminated at the nonreducing end by 2,3-di-O-methylrhamnose and 3-O-methylxylose residues.
Lee
J B
JB
Faculty of Pharmaceutical Sciences and School of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
Hayashi
T
T
Hayashi
K
K
Sankawa
U
U
Maeda
M
M
Nemoto
T
T
Nakanishi
H
H
D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D000458:Cyanobacteria / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D018259:Herpesvirus 1, Human / Q000187:drug effects; D004854:Herpesvirus 4, Human; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
D000998:Antiviral Agents; D011134:Polysaccharides; C102617:spirulan