title: Lack of active lung anaphylaxis in congenitally mast cell-deficient Ws/Ws rats sensitized with the nematode Nippostrongylus brasiliensis.
abstract: Ws/Ws rats are deficient in both mucosal- and connective tissue-type mast cells. To study the role of mast cells in active anaphylaxis, changes in vascular permeability in the trachea upon intravenous antigen challenge with Evans blue dye were examined in Ws/Ws, heterogenic Ws/+, and normal +/ + rats sensitized with the nematode Nippostrongylus brasiliensis. Antigen challenge resulted in fatal anaphylactic shock in some +/+ and Ws/+ rats, but not in Ws/Ws rats. Marked dye leakage developed within 30 min in the trachea of +/+ and Ws/+ rats, while Ws/Ws rats showed no substantial increases in the levels of vascular permeability. Ex vivo stimulation of sensitized lung fragments from +/+ animals with specific antigen induced significant releases of histamine and leukotriene (LT) C4, while sensitized Ws/Ws rat-lung fragments did not. In Ws/Ws rats, levels of nematode-specific IgE, IgG1 and IgG2a antibodies as well as levels of lung eosinophilia were not significantly different from those in +/+ rats. These results show that mast cell-deficient Ws/Ws rats fail to develop active anaphylaxis, and this is mediated probably by the lack of mast cell-derived mediators required for initiation of the reaction.
authors.affiliation: Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nishida
authors.forename: R
authors.initials: R
authors.lastname: Uchikawa
authors.forename: T
authors.initials: T
authors.lastname: Tegoshi
authors.forename: M
authors.initials: M
authors.lastname: Yamada
authors.forename: S
authors.initials: S
authors.lastname: Matsuda
authors.forename: M
authors.initials: M
authors.lastname: Sasabe
authors.forename: N
authors.initials: N
authors.lastname: Arizono
mesh_terms: D000707:Anaphylaxis / Q000276:immunology* / Q000469:parasitology*; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology; D002199:Capillary Permeability; D002452:Cell Count; D006636:Histamine Release; D007114:Immunization; D017997:Leukotriene C4 / Q000378:metabolism; D008168:Lung / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology* / Q000473:pathology; D008407:Mast Cells / Q000276:immunology* / Q000469:parasitology* / Q000473:pathology; D009559:Nippostrongylus / Q000276:immunology*; D051381:Rats; D011922:Rats, Mutant Strains; D017206:Strongylida Infections / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D014132:Trachea / Q000098:blood supply
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D017997:Leukotriene C4