title: Some bronchoalveolar lavage parameters and leukocyte cytokine release in response to intratracheal instillation of short and long asbestos and wollastonite fibres in rats.
abstract: We investigated the differences between the lavage parameters -- including tumour necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) release by lavage leukocytes -- in control rats and in animals intratracheally instilled with short and long amosite and wollastonite fibres. These cytokines can play an important role in lung disease development after long-term exposure to some fibrous dusts. Short and long amosite and wollastonite fibres were intratracheally instilled in rats (1 mg/week) for ten weeks while saline was given to controls. To compare the harmful effects of these fibres, the number of leukocytes/ml of bronchoalveolar lavage (BAL), the number of alveolar macrophages (AM) per ml of BAL, AM:granulocyte (GR) ratios in lavage fluid, phagocytic activity and viability of AM, lactate dehydrogenase (LDH), acid phosphatase (AcP), and TNF-alpha and IFN-gamma release by lavage leukocytes were investigated 3 months after the first intratracheal instillation. Compared with the controls, amosite short fibres significantly decreased the numbers of AM/ml BAL, and increased their phagocytic activity and AcP release. Long amosite fibres significantly decreased the numbers of AM/ml BAL, increased the number of granulocytes depressed the phagocytic activity and viability of AM, and significantly decreased the levels of TNF-alpha and IFN-gamma in supernatants of cultured leukocytes. While wollastonite short and long fibre instillation did not significantly influence the parameters studied (except for a significantly increased number of leukocytes/ml BAL in wollastonite long fibres), amosite short and long fibres caused marked differences in these parameters, the long fibres being more effective.
authors.affiliation: Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Hurbánková
authors.forename: A
authors.initials: A
authors.lastname: Kaiglová
mesh_terms: D000818:Animals; D017639:Asbestos, Amosite / Q000633:toxicity*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry* / Q000166:cytology; D017610:Calcium Compounds / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D005260:Female; D007371:Interferon-gamma / Q000378:metabolism; D007962:Leukocytes / Q000737:chemistry*; D016676:Macrophages, Alveolar / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D017640:Silicates / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D017610:Calcium Compounds; D002273:Carcinogens; D017640:Silicates; D014409:Tumor Necrosis Factor-alpha; D017639:Asbestos, Amosite; D007371:Interferon-gamma; C031293:calcium silicate