title: Rebamipide prevented delay of wound repair induced by hydrogen peroxide and suppressed apoptosis of gastric epithelial cells in vitro.
abstract: The effects of rebamipide on the restoration process of gastric epithelial wounds were assessed using an in vitro wound-healing model and hydrogen peroxide treatment. Rebamipide (10 or 100 microM) was added to a complete monolayer cell sheet after artificial wounding. The restoration process was analyzed sequentially by a time-lapse video system, and cell migration, proliferation, and apoptosis were assessed. Hydrogen peroxide (1 or 3 mM) inhibited restoration after wounding by suppressing cell migration and proliferation. It also induced epithelial cell apoptosis around the wound. The addition of rebamipide abolished the H2O2-induced retardation and prevented apoptosis. Rebamipide might act as a radical scavenger and have favorable effects on peptic ulcer diseases.
authors.affiliation: Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Watanabe
authors.forename: X E
authors.initials: XE
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Hirose
authors.forename: T
authors.initials: T
authors.lastname: Osada
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: N
authors.initials: N
authors.lastname: Sato
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004848:Epithelium / Q000187:drug effects; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects*; D006861:Hydrogen Peroxide / Q000009:adverse effects*; D066298:In Vitro Techniques; D016877:Oxidants / Q000009:adverse effects*; D015363:Quinolones / Q000494:pharmacology*; D011817:Rabbits; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D016877:Oxidants; D015363:Quinolones; D006861:Hydrogen Peroxide; C052785:rebamipide; D000409:Alanine