Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to identify such genes and show by whole-mount in situ hybridisation that their expression pattern is that expected from the differential display profile.
Gupta
R
R
Division of Eukaryotic Molecular Genetics and Division of Mammalian Development,MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK.
Thomas
P
P
Beddington
R S
RS
Rigby
P W
PW
D000818:Animals; D018076:DNA, Complementary / Q000032:analysis* / Q000235:genetics*; D005314:Embryonic and Fetal Development / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D015723:Gene Library; D051379:Mice; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy
D018076:DNA, Complementary