A protease inhibitor attenuates gastric erosions and microcirculatory disturbance in the early period after thermal injury in rats.
The effects of camostat mesilate, a synthetic serine protease inhibitor on gastric microcirculation and active oxygen species generated by leucocytes from the gastric and jugular veins in the early period after thermal injury were assessed. Male Wistar rats were anaesthetized and a 30% full skin-thickness dorsal burn was inflicted. Camostat mesilate (100 mg/kg) was dissolved in distilled water and administered orally to rats 40 min before thermal injury (the camostat group). The control animals (the vehicle group) were administered distilled water orally. Rolling leucocytes as well as Monastral blue B deposits in venules were observed using in vivo microscopy. Active oxygen species were measured by chemiluminescence. Camostat mesilate decreased the total length of gastric erosion, venular deposits of Monastral blue B, and rolling of leucocytes in venules, and relatively increased luminol-dependent chemiluminescence activity generated by zymosan-stimulated leucocytes 15 min after thermal injury. These results suggest that serine proteases are involved in the formation of gastric erosions and gastric microcirculatory disturbance in the early period after thermal injury.
Yoshida
M
M
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
Wakabayashi
G
G
Ishikawa
H
H
Otani
Y
Y
Shimazu
M
M
Kubota
T
T
Kumal
K
K
Kurose
I
I
Miura
S
S
Ishii
H
H
Kitajima
M
M
D000818:Animals; D002056:Burns / Q000503:physiopathology*; D004952:Esters; D005609:Free Radicals; D016670:Gabexate / Q000031:analogs & derivatives*; D005753:Gastric Mucosa; D006146:Guanidines / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008833:Microcirculation; D011480:Protease Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000098:blood supply / Q000187:drug effects* / Q000473:pathology
D004952:Esters; D005609:Free Radicals; D006146:Guanidines; D011480:Protease Inhibitors; C034532:camostat; D016670:Gabexate