Protection of PC12 cells glutathione peroxidase in L-DOPA induced cytotoxicity.
L-DOPA may cause side-effects during the treatment of Parkinson's disease. We investigated the role of glutathione peroxidase (GSHPx) in cellular defense against L-DOPA cytotoxicity. A line of PC12 cells overexpressing GSHPx with plasmid pRc/CMV-GSHPx was established and stable transfectants overexpressing GSHPx were used for this study. GSHPx activity was found to be 1.5-fold higher in GSHPx-transfectants than in mock-controlled transfectants. Transfectants over expressing GSHPx were also significantly more resistant to exposure to either L-DOPA or t-butyl hydroperoxide than mock-transfected cells. Results suggested that L-DOPA may cause neuronal cell death by an oxidative pathway and GSHPx may play an important role in cellular defense against oxidative stress.
Kim-Han
J S
JS
Department of Pharmacology, University of Missouri, Columbia 65212, USA.
Sun
A Y
AY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016923:Cell Death; D019610:Cytoprotection; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism*; D007980:Levodopa / Q000633:toxicity*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D016716:PC12 Cells; D051381:Rats; D014162:Transfection; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000975:Antioxidants; D007980:Levodopa; D020122:tert-Butylhydroperoxide; D005979:Glutathione Peroxidase; D005978:Glutathione