Analysis of the 16S rRNA gene sequence of the coryneform bacterium associated with hyperkeratotic dermatitis of athymic nude mice and development of a PCR-based detection assay.
By 16S rDNA sequencing the authors have characterized the coryneform bacteria associated with hyperkeratotic dermatitis (HD) of athymic nude mice isolated from six different outbreaks of the disease in Northern Italy. This analysis has allowed the authors to confirm the classification of the bacteria as Corynebacterium bovis and to develop a 16S rDNA-based polymerase chain reaction (PCR) detection assay. The test was performed directly on the DNA extracted from epidermal swabs. The PCR primers were chosen to match the 16S rDNA sequence fragments which differ most from the other Corynebacterium spp. The test was shown to be both sensitive and specific for C. bovis. Detection of as few as three viable bacterial cells was possible with the use of an oligonucleotide probe in a liquid hybridization assay.
Duga
S
S
Istituto di Fisiologia Veterinaria e Biochimica, Università di Milano, Italy.
Gobbi
A
A
Asselta
R
R
Crippa
L
L
Tenchini
M L
ML
Simonic
T
T
Scanziani
E
E
D000818:Animals; D001483:Base Sequence; D003352:Corynebacterium / Q000235:genetics* / Q000302:isolation & purification; D003354:Corynebacterium Infections / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*; D005798:Genes, Bacterial; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics*; D012376:Rodent Diseases / Q000235:genetics / Q000382:microbiology*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D012867:Skin / Q000382:microbiology; D017192:Skin Diseases, Bacterial / Q000235:genetics / Q000382:microbiology / Q000662:veterinary*
D012336:RNA, Ribosomal, 16S