Immunochemical approaches to AGE-structures: characterization of anti-AGE antibodies.
Recent immunological approaches have greatly helped broaden our understanding of the biomedical significance of advanced glycation end products (AGEs) in aging and age-enhanced disease processes. Recently, Nepsilon-(carboxymethyl) lysine (CML), one of the glycoxidation products of AGEs, was demonstrated to be a major immunological epitope among AGEs. In the subsequent study, we characterized 13 different polyclonal anti-AGE antibodies and showed that these antibodies could be classified into three groups (Groups I, II and III). Group I was specific for CML and both Group II and Group III were specific for other epitopes (non-CML). Time-course study suggested that the epitope of Group II was formed earlier than that of Group III. In the present study, we prepared two monoclonal anti-AGE antibodies (2A2 and 3A3) whose epitope structures appeared to be closely related to Group III and Group II, respectively. The result indicates that AGE-proteins express at least two major non-CML epitopes.
Ikeda
K
K
Department of Biochemistry, Kumamoto University, School of Medicine, Honjo, Japan.
Nagai
R
R
Sakamoto
T
T
Sano
H
H
Araki
T
T
Sakata
N
N
Nakayama
H
H
Yoshida
M
M
Ueda
S
S
Horiuchi
S
S
D000818:Animals; D000906:Antibodies / Q000032:analysis* / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis; D017127:Glycation End Products, Advanced / Q000032:analysis / Q000276:immunology*; D007150:Immunohistochemistry; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000939:Epitopes; D017127:Glycation End Products, Advanced; C048496:N(6)-carboxymethyllysine; D008239:Lysine