Is PGL-1 also present in Leishmania donovani promastigotes?
A soluble antigen complex (SAC) derived from the ruptured promastigotes of Leishmania donovani parasites (LD-SAC) was used for complement fixation test (CFT) in leprosy Cases of tuberculoid and borderline tuberculoid leprosy, post-kala azar dermal leishmaniasis (TT, BT, PKDL) and control sera gave negative CFT. Smear-positive cases of borderline (BB, BL) and lepromatous (LL) leprosy and drug-resisting cases of pulmonary tuberculosis gave positive CFT; smear-negative cases of LL leprosy sera also gave positive CFT. Sera of smear-negative inactive LL patients contained only PGL-1 and PDIM antigens for a long time after they become inactive. Therefore, the positive CFT in inactive LL makes us suspect whether PGL-1 is present in LD promastigotes.
Gupta
P N
PN
Department of Leprology, School of Tropical Medicine, Calcutta.
Pal
N K
NK
D000328:Adult; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D003168:Complement Fixation Tests; D003429:Cross Reactions; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007893:Leishmania donovani / Q000254:growth & development / Q000276:immunology*; D007898:Leishmaniasis, Visceral / Q000276:immunology; D007918:Leprosy / Q000175:diagnosis* / Q000276:immunology; D015439:Leprosy, Borderline / Q000175:diagnosis / Q000276:immunology; D015440:Leprosy, Lepromatous / Q000175:diagnosis / Q000276:immunology; D015441:Leprosy, Tuberculoid / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009166:Mycobacterium leprae / Q000276:immunology*; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis
D000942:Antigens, Bacterial; D000953:Antigens, Protozoan; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae