Time-resolved spectroscopy of mitochondria, cells and tissues under normal and pathological conditions.
In this study, the detailed dependence of light scattering on tissue architecture and intracellular composition has been investigated. Firstly, we simulated the reduced scattering coefficient (mu(s)') of the rat liver using the Mie theory, the Rayleigh-Debye-Gans approximation and electron microscopy data. Then, the reduced scattering coefficient of isolated rat liver mitochondria, isolated hepatocytes and various rat tissues (i.e. perfused liver, brain, muscle, tumors) was measured at 780 nm by using time-resolved spectroscopy and a sample-substitution protocol. The comparison of the isolated mitochondria data with the isolated hepatocyte and whole liver measurements suggests that the mitochondrial compartment is the primary factor for light propagation in hepatic tissue, thus strengthening the relevance of the preliminary theoretical study. Nevertheless, the possibility that other intracellular components, such as peroxisomes and lysosomes, interfere with light propagation in rat liver is discussed. Finally, we demonstrate that light scattering in normal rat tissues and tumors is roughly proportional to the mitochondrial content, according to estimates of the mitochondrial protein content of the tissues.
Beauvoit
B
B
Institut de Biochimie et Génétique Cellulaires du CNRS, Université de Bordeaux II, France.
Chance
B
B
D002458:Cell Fractionation; D002460:Cell Line; D008099:Liver / Q000166:cytology / Q000201:enzymology; D008930:Mitochondria, Liver / Q000378:metabolism*; D009369:Neoplasms / Q000473:pathology; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*