Influence of dextran molecular weight on capture in and release from decylamine carboxymethylcellulose capsules.
A series of dextran molecular weight markers were encapsulated in decylamine carboxymethylcellulose microcapsules to serve as probes of capsule retentivity. The capsules were prepared by allowing microdrops of aqueous sodium carboxymethylcellulose to fall into aqueous decylamine acetate solution. Salt exchange reaction at the droplet pseudointerface resulted in self-assembling films which essentially instantaneously enclosed the droplets. Concentrations of anionic polymer were varied in the range from 1-3%. Chromophore-bearing dextrans were incorporated into these capsules by blending the dextrans with the carboxymethylcellulose prior to the encapsulation step. Four dextrans of differing (light scattering) molecular weights were used: 2 x 10(6), 6 x 10(5), 7 x 10(4), and 1.9 x 10(4) amu. The mass balance of dextran retained in the capsules, released on washing the capsules or which escaped encapsulation was determined spectrophotometrically. To measure total dextran in a population of washed capsules, the capsules were lysed in a 0.3 M solution of sodium chloride. To monitor dextran release, washed capsules were suspended in water and dextran concentration in the supernatant was measured. Encapsulation efficiency exceeded 80% for high molecular weight dextran but was lower with the smaller dextrans.
Mathew
E
E
Temple University School of Pharmacy, Philadelphia, PA 19140, USA.
Speaker
T J
TJ
D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D002214:Capsules; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003911:Dextrans / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D007700:Kinetics; D008970:Molecular Weight; D010592:Pharmaceutic Aids / Q000737:chemistry*
D000588:Amines; D002214:Capsules; D003911:Dextrans; D010592:Pharmaceutic Aids; C015219:fluorescein isothiocyanate dextran; D016650:Fluorescein-5-isothiocyanate; D002266:Carboxymethylcellulose Sodium; C004744:decylamine