title: Infrequent somatic mutation of the adenomatous polyposis coli gene in aberrant crypt foci of human colon tissue.
abstract: BACKGROUND
  The authors examined somatic mutations of the adenomatous polyposis coli (APC) gene in 84 human aberrant crypt foci (ACF) to determine whether APC gene mutations were involved in the histologic progression of ACF.
  
  
  METHODS
  Mutation cluster regions of the APC gene were subjected to polymerase chain reaction single-strand conformation polymorphism analysis and direct sequencing.
  
  
  RESULTS
  Four kinds of deletion were detected in the mutation cluster regions of APC gene in five ACF. APC mutation was detected in 1 of 18 ACF with Stage I abnormalities (6%). Four of 10 adenomatous ACF (40%) harbored the mutation. There were no mutations in 56 hyperplastic ACF.
  
  
  CONCLUSIONS
  These results suggest that APC mutations may be involved initially in only a limited number of adenomas in ACF.
authors.affiliation: Investigative Treatment Division, National Cancer Center Research Institute East, Kashiwa, Chiba, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Otori
authors.forename: M
authors.initials: M
authors.lastname: Konishi
authors.forename: K
authors.initials: K
authors.lastname: Sugiyama
authors.forename: T
authors.initials: T
authors.lastname: Hasebe
authors.forename: T
authors.initials: T
authors.lastname: Shimoda
authors.forename: R
authors.initials: R
authors.lastname: Kikuchi-Yanoshita
authors.forename: K
authors.initials: K
authors.lastname: Mukai
authors.forename: S
authors.initials: S
authors.lastname: Fukushima
authors.forename: M
authors.initials: M
authors.lastname: Miyaki
authors.forename: H
authors.initials: H
authors.lastname: Esumi
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics*; D000368:Aged; D000369:Aged, 80 and over; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D017353:Gene Deletion; D006801:Humans; D007413:Intestinal Mucosa / Q000737:chemistry* / Q000473:pathology*; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational