Transformation of the Lyme disease spirochete Borrelia burgdorferi with heterologous DNA.
Studies of the spirochete Borrelia burgdorferi have been hindered by the scarcity of genetic tools that can be used in these bacteria. For the first time, a method has been developed by which heterologous DNA (DNA without a naturally occurring B. burgdorferi homolog) can be introduced into and persistently maintained by B. burgdorferi. This technique uses integration of circular DNA into the bacterial genome via a single-crossover event. The ability to transform B. burgdorferi with heterologous DNA will now permit a wide range of experiments on the biology of these bacteria and their involvement in the many facets of Lyme disease.
Stevenson
B
B
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA. bstev0@pop.uky.edu
Bono
J L
JL
Elias
A
A
Tilly
K
K
Rosa
P
P
D025065:Borrelia burgdorferi; D015748:Borrelia burgdorferi Group / Q000235:genetics*; D005822:Genetic Vectors; D007613:Kanamycin Resistance / Q000235:genetics; D014169:Transformation, Bacterial