Conjugates of Tremella polysaccharides with microbeads and their TNF-stimulating activity.
A mannan (A) and a heteroglycan (D) were prepared by partial acidic hydrolysis of T3, a major polysaccharide fraction of the fungus Tremella fuciformis Berk. Methylation analysis of A and D showed that they contained a 1-->3 linked mannosyl main chain, to which were linked different side chains at position 2, 4, or 6 of the mannosyl residues. A and D were conjugated to albumin microbeads (AM) by reductive amination. The conjugates showed significant cytokine-stimulating activity in vitro whereas unconjugated AM had no activity. Not conjugated A and D showed cytokine-stimulating activity only in about 100 times higher concentrations.
Gao
Q
Q
Department of Experimental Pathology, IMB, University of Tromsø, Norway.
Berntzen
G
G
Jiang
R
R
Killie
M K
MK
Seljelid
R
R
D001487:Basidiomycota; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D003911:Dextrans; D006801:Humans; D066298:In Vitro Techniques; D008351:Mannans / Q000737:chemistry* / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D008969:Molecular Sequence Data; D009000:Monocytes / Q000187:drug effects / Q000276:immunology*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D003911:Dextrans; D008351:Mannans; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; C025614:sephadex