Rapid detection of rifampin-resistant Mycobacterium tuberculosis by sequencing and line probe assay.
Resistance to rifampin (RMP) is associated with mutations in the rpoB gene. Disk elution method, direct DNA sequencing and line probe assay were compared in rapid detection of rpoB mutations from 30 Mycobacterium tuberculosis isolates. Concordance between LiPA and culture was 93.3%, whereas sequencing yielded 100% concordance with culture. These results indicate that line probe assay is nearly as sensitive a method as sequencing in rapid detection of RMP-resistance of M. tuberculosis isolates, and can be applied in laboratories working with standard PCR equipment.
Marttila
H J
HJ
Mycobacterial Reference Laboratory, Turku Immunology Centre, National Public Health Institute, Finland.
Soini
H
H
Vyshnevskiy
B I
BI
Otten
T F
TF
Vasilyef
A V
AV
Huovinen
P
P
Viljanen
M K
MK
D000904:Antibiotics, Antitubercular / Q000494:pharmacology*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis*; D004352:Drug Resistance, Microbial; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity
D000904:Antibiotics, Antitubercular; D004269:DNA, Bacterial; D012293:Rifampin