Comparison of bone regeneration in a rabbit skull defect by recombinant human BMP-2 incorporated in biodegradable hydrogel and in solution.
The objective of this study is to compare bone regeneration induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) incorporated into a biodegradable gelatin hydrogel with that by rhBMP-2 in aqueous solution. After treating rabbit skull defects of 6 mm diameter with the two rhBMP-2 dosage forms, both of them increased the bone mineral density (BMD) at the skull defects with implantation time to a significantly higher extent than a rhBMP-2-free aqueous solution and a rhBMP-2-free empty gelatin hydrogel (p < 0.05). There was no quantifiable difference in BMD between the two dosage forms of rhBMP-2. Histological examination revealed that the integrity of newly generated bone increased with the rhBMP-2 dose, irrespective of the dosage form. The bone defect was filled with regenerated bone 21 days after treatment.
Hong
L
L
Department of Neurosurgery, Kyoto University Medical School, Japan.
Tabata
Y
Y
Yamamoto
M
M
Miyamoto
S
S
Yamada
K
K
Hashimoto
N
N
Ikada
Y
Y
D015502:Absorptiometry, Photon; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D015519:Bone Density / Q000187:drug effects / Q000502:physiology; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology* / Q000627:therapeutic use; D001861:Bone Regeneration / Q000187:drug effects* / Q000502:physiology; D005780:Gelatin / Q000737:chemistry; D005782:Gels; D010006:Osteoblasts / Q000502:physiology; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology / Q000627:therapeutic use; D012886:Skull / Q000293:injuries* / Q000502:physiology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000494:pharmacology* / Q000627:therapeutic use
C525718:BMP2 protein, human; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D005782:Gels; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D005780:Gelatin