Frequent somatic mutations in serine/threonine kinase 11/Peutz-Jeghers syndrome gene in left-sided colon cancer.
We analyzed somatic mutation and loss of heterozygosity (LOH) in the serine/threonine kinase 11 (STK11)/Peutz-Jeghers syndrome gene in 49 colorectal tumors in three different stages of a dysplasia-carcinoma sequence. We detected LOH in 10 of 19 (52.6%) informative colorectal cancers at loci D19S886 and/or D19S883, but no LOH was observed in 25 informative adenomas. We detected a total of 9 somatic mutations [7 of 13 (53.8%) left-sided colon cancers and 2 of 7 (28.6%) left-sided adenomas with high-grade dysplasia], but no mutations were detected in right-sided colon tumors. Of the nine mutations, one was a frameshift mutation (the same mutation detected in Peutz-Jeghers syndrome family previously), and the other eight were missense mutations. This results indicate that STK11 is a tumor suppressor gene and that genetic changes of STK11 play an important role in left-sided colon cancer carcinogenesis.
Dong
S M
SM
Department of Pathology and Cancer Research Institute, Catholic University Medical College, Seoul, Korea.
Kim
K M
KM
Kim
S Y
SY
Shin
M S
MS
Na
E Y
EY
Lee
S H
SH
Park
W S
WS
Yoo
N J
NJ
Jang
J J
JJ
Yoon
C Y
CY
Kim
J W
JW
Kim
S Y
SY
Yang
Y M
YM
Kim
S H
SH
Kim
C S
CS
Lee
J Y
JY
D000091162:AMP-Activated Protein Kinase Kinases; D001483:Base Sequence; D002888:Chromosomes, Human, Pair 19; D003110:Colonic Neoplasms / Q000235:genetics*; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008969:Molecular Sequence Data; D009154:Mutation; D010580:Peutz-Jeghers Syndrome / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases