Effect of bacterial invasion of macrophages on the outcome of assays to assess bacterium-macrophage interactions.
In vitro assays to quantify killing of bacteria by macrophages provide useful insights into host-pathogen relations. In the present study, we used strains of Yersinia enterocolitica and Escherichia coli which varied in their ability to invade mammalian cells to evaluate these assays. The results showed that 30 min and 24 h after incubation with murine bone marrow-derived macrophages, strains of Y. enterocolitica and E. coli which expressed invasin (an outer membrane protein which allows bacteria to penetrate mammalian cells) achieved significantly greater numbers in macrophages than otherwise isogenic bacteria which lacked this protein (P < 0.01). When the 24-h data were corrected for the number of bacteria ingested by macrophages initially, the differences between invasin-positive and -negative bacteria were no longer evident (P> 0.2). This study has shown (1) that invasin-mediated penetration of macrophages by bacteria is not associated with enhanced intracellular survival, and (2) that invasion of macrophages by bacteria may influence the interpretation of assays for bactericidal capacity unless allowance is made for the number of bacteria ingested during the early phase of the assay.
de Koning-Ward
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TF
Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Parkville, Victoria, Australia.
Grant
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Oppedisano
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Robins-Browne
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RM
D000818:Animals; D001770:Blood Bactericidal Activity; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology* / Q000472:pathogenicity; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D015008:Yersinia enterocolitica / Q000276:immunology* / Q000472:pathogenicity