News & notes. Efficient library construction with a TA vector and its application to cloning of the phytoene synthase gene from the cyanobacterium Spirulina platensis.
An efficient and simple method for constructing a genomic DNA library is presented by use of a TA cloning vector. It is based on sonicative cleavage of genomic DNA and modification of the fragment ends with Taq DNA polymerase, followed by ligation with a TA vector. This method was successfully applied to cloning of the phytoene synthase gene crtB from Spirulina platensis. The method is useful when the genomic DNA is not well digested with restriction enzymes owing to methylation or other reasons.
Kawata
Y
Y
Osaka National Research Institute, Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan.
Yano
S
S
Kojima
H
H
D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D015698:Genomic Library; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase; D008969:Molecular Sequence Data
D017931:DNA Primers; D004269:DNA, Bacterial; D019883:Alkyl and Aryl Transferases; D051232:Geranylgeranyl-Diphosphate Geranylgeranyltransferase