Polymer surface chemistry and bone cell migration.
Implant devices for orthopaedic applications may be improved if the surface of the biomaterial provides for osteointegration. To understand the effect of hydrophilicity on colonisation by human bone derived (HBD) cells, we compared untreated polystyrene (PS) and a sulfuric acid-treated PS surface for mechanisms of cell migration. The chemical composition of the acid-treated PS surface was analysed by monochromatic X-ray photoelectron spectroscopy and found to contain various oxidatively produced groups and a minor amount of sulfonate groups. It was found that migration of HBD cells on both PS and acid-treated PS surface was dependent on the presence of vitronectin (Vn) and was higher on the hydrophilic acid-treated surface. Minimal migration of HBD cells occurred on either surface in the absence of Vn, even when fibronectin was present in the culture medium. Using radiolabelled protein, it was shown that Vn adsorption onto the acid-treated surface was two to three fold greater than that on the hydrophobic PS. When HBD cells were seeded onto a patterned surface in a medium containing Vn, the cells preferentially colonised the hydrophilic region and few, if any, cells traversed the haptotactic boundary from the hydrophilic to the hydrophobic side. Thus the enhanced HBD cell migration seen on the acid-treated PS compared with the untreated PS surface and the haptotactic boundary phenomenon, relate to Vn adsorption.
Dalton
B A
BA
CSIRO, Molecular Science, Sydney Laboratories, North Ryde, Australia.
McFarland
C D
CD
Gengenbach
T R
TR
Griesser
H J
HJ
Steele
J G
JG
D000293:Adolescent; D001672:Biocompatible Materials / Q000737:chemistry*; D001842:Bone and Bones / Q000166:cytology*; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D002648:Child; D004577:Electron Probe Microanalysis; D005353:Fibronectins / Q000494:pharmacology; D006801:Humans; D011137:Polystyrenes / Q000737:chemistry*; D013464:Sulfuric Acids; D013499:Surface Properties; D019096:Vitronectin / Q000494:pharmacology
D001672:Biocompatible Materials; D005353:Fibronectins; D011137:Polystyrenes; D013464:Sulfuric Acids; D019096:Vitronectin; C033158:sulfuric acid