Expression of pI(Cln) in Escherichia coli gives a strong tolerance to hypotonic stress.
We amplified the coding region DNA sequence from a rat renal pI(Cln) cDNA by PCR and expressed the protein in Escherichia coli cells. The cells were exposed to hypotonic conditions followed by spreading them onto LB plates for subsequent colony survival assay. The present study demonstrated that the cells expressing pI(Cln) exhibit a strong resistance to hypotonic stress. Moreover, the resistance was specifically inhibited by extracellular ATP and some anion channel inhibitors. These findings indicate that the expression of pI(Cln) directly confers tolerance to hypotonic stress, and pI(Cln) is concluded to be an important molecule for cell-volume regulation.
Tao
G Z
GZ
Department-2 of Biochemistry, School of Medicine, Akita University, Japan.
Kobayashi
A
A
Itoh
H
H
Tashima
Y
Y
D000220:Adaptation, Biological / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018118:Chloride Channels / Q000502:physiology*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D007038:Hypotonic Solutions; D008969:Molecular Sequence Data; D010957:Plasmids; D051381:Rats
D018118:Chloride Channels; D007038:Hypotonic Solutions