Store-operated calcium entry promotes shape change in pulmonary endothelial cells expressing Trp1.
Activation of Ca2+ entry is known to produce endothelial cell shape change, leading to increased permeability, leukocyte migration, and initiation of angiogenesis in conduit-vessel endothelial cells. The mode of Ca2+ entry regulating cell shape is unknown. We hypothesized that activation of store-operated Ca2+ channels (SOCs) is sufficient to promote cell shape change necessary for these processes. SOC activation in rat pulmonary arterial endothelial cells increased free cytosolic Ca2+ that was dependent on a membrane current having a net inward component of 5.45 +/- 0.90 pA/pF at -80 mV. Changes in endothelial cell shape accompanied SOC activation and were dependent on Ca2+ entry-induced reconfiguration of peripheral (cortical) filamentous actin (F-actin). Because the identity of pulmonary endothelial SOCs is unknown, but mammalian homologues of the Drosophila melanogaster transient receptor potential (trp) gene have been proposed to form Ca2+ entry channels in nonexcitable cells, we performed RT-PCR using Trp oligonucleotide primers in both rat and human pulmonary arterial endothelial cells. Both cell types were found to express Trp1, but neither expressed Trp3 nor Trp6. Our study indicates that 1) Ca2+ entry in pulmonary endothelial cells through SOCs produces cell shape change that is dependent on site-specific rearrangement of the microfilamentous cytoskeleton and 2) Trp1 may be a component of pulmonary endothelial SOCs.
Moore
T M
TM
Department of Pharmacology, College of Medicine, University of South Alabama, Mobile, Alabama 36688, USA.
Brough
G H
GH
Babal
P
P
Kelly
J J
JJ
Li
M
M
Stevens
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000878:Anthranilate Synthase / Q000235:genetics; D001483:Base Sequence; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D017931:DNA Primers; D004331:Drosophila melanogaster; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics; D007700:Kinetics; D008297:Male; D008322:Mammals; D008564:Membrane Potentials; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D018408:Patch-Clamp Techniques; D016133:Polymerase Chain Reaction; D011651:Pulmonary Artery; D051381:Rats; D017207:Rats, Sprague-Dawley; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D050052:TRPC Cation Channels; D019284:Thapsigargin / Q000494:pharmacology
D015220:Calcium Channels; D017931:DNA Primers; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; D050052:TRPC Cation Channels; C096351:transient receptor potential cation channel, subfamily C, member 1; D019284:Thapsigargin; D007209:Indole-3-Glycerol-Phosphate Synthase; D000878:Anthranilate Synthase; C087439:TRP3 protein, S cerevisiae; D002118:Calcium