Sensitivity of cross-reacting antihuman antibodies in formalin-fixed porcine skin: including antibodies to proliferation antigens and cytokeratins with specificity in the skin.
Although no animal is a perfect skin model for the study of toxicological and therapeutic agents, structurally the pig may be superior to even non-human primates. Because our work involves effects of toxicological and therapeutic agents on the skin, we wanted to identify stains which may prove useful as well as determine cross-reactivity of some newer antihuman antibodies. We performed a battery of formalin-fixed skin from weanling pigs and minipigs. The battery of antibodies included LCA, CD3, OPD-4, CD34, UCHL-1, L-26, KP-1, MAC-387, Factor XIIIa, Leu-7, S-100 protein, HMB-45, GFAP, synaptophysin, neurofilament protein, ubiquitin, vimentin, type IV collagen, laminin, fibronectin, Factor VIII related antigen, Desmin-M, smooth muscle actin, cytokeratin 7, cytokeratin 20, AEI/AE3, CAM 5.2, EMA, GCDFP, Ki-67, and PCNA. Immunohistochemical stains for CD3, Leu-7, S-100 protein, type IV collagen, laminin, Factor VIII related antigen, GFAP, synaptophysin, neurofilament protein, ubiquitin, smooth muscle actin, vimentin, Desmin-M, cytokeratin 7, cytokeratin 20, AE1/AE3, CAM 5.2, Ki-67 and PCNA showed consistent cross-reactivity. In formalin-fixed tissue, only antibodies to lymphoreticular cells showed poor cross-reactivity. A high percentage of the remaining antibodies did show good cross-reactivity but with some interesting similarities and differences in specificity.
Smith
K J
KJ
Medical Research Institute of Chemical Defense, Aberdeen, MD, USA.
Graham
J S
JS
Skelton
H G
HG
Hamilton
T
T
O'Leary
T
T
Okerberg
C V
CV
Moeller
R
R
Hurst
C G
CG
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D015415:Biomarkers; D002455:Cell Division / Q000276:immunology; D003429:Cross Reactions; D005557:Formaldehyde; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000166:cytology / Q000276:immunology; D007633:Keratins / Q000276:immunology*; D019394:Ki-67 Antigen / Q000276:immunology*; D018809:Proliferating Cell Nuclear Antigen / Q000276:immunology*; D012867:Skin / Q000166:cytology* / Q000276:immunology*; D013045:Species Specificity; D013552:Swine; D016707:Tissue Fixation
D000906:Antibodies; D015415:Biomarkers; D019394:Ki-67 Antigen; D018809:Proliferating Cell Nuclear Antigen; D005557:Formaldehyde; D007633:Keratins