title: Dose-dependent response of vestibular hair cells of guinea pigs following streptomycin ototoxiation.
abstract: Although the involvement of apoptosis has been suggested in the loss of vestibular hair cells due to aminoglycosides, dose-dependent effects of aminoglycosides have not been determined. We therefore examined dose-dependent effects of streptomycin on the degeneration of hair cells of guinea pig ampullar cristae using TUNEL stain and Hoechst nuclear stain. Streptomycin induced apoptosis of hair cells in a dose-dependent manner. Even following high-dose applications, most of the affected cells showed apoptotic features. Apoptosis may therefore play a predominant role in the deletion of vestibular hair cells affected by aminoglycosides.
authors.affiliation: Department of Otorhinolaryngology of Yodogawa Christian Hospital, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakagawa
authors.forename: H
authors.initials: H
authors.lastname: Yamane
authors.forename: M
authors.initials: M
authors.lastname: Takayama
authors.forename: K
authors.initials: K
authors.lastname: Sunami
authors.forename: Y
authors.initials: Y
authors.lastname: Nakai
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D001562:Benzimidazoles; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes; D006168:Guinea Pigs; D018069:Hair Cells, Vestibular / Q000187:drug effects*; D020287:In Situ Nick-End Labeling; D013307:Streptomycin / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D001562:Benzimidazoles; D005456:Fluorescent Dyes; C017807:bisbenzimide ethoxide trihydrochloride; D013307:Streptomycin