Competitive binding assay for thyroxine using in vitro selected oligonucleotides.
A new binding assay that uses oligodeoxyribonucleotides (DNAs) obtained by the in vitro selection method, instead of antibodies, to bind to the target molecule, thyroxine (T4), is described. The DNAs which selectively bound to the T4 were selected, labeled with biotin or radioisotope, and then used to detection of T4 in the presence of liothyronine (T3), which has a chemical structure similar to that of T4.
Ito
Y
Y
Graduate School of Materials Science, NAIST, Ikoma, Japan.
Fujita
S
S
Kawazoe
N
N
Imanishi
Y
Y
D015374:Biosensing Techniques; D017931:DNA Primers; D006801:Humans; D066298:In Vitro Techniques; D009841:Oligonucleotides; D013974:Thyroxine / Q000737:chemistry*
D017931:DNA Primers; D009841:Oligonucleotides; D013974:Thyroxine