title: Studies of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Effect of inhibitors and low temperature.
abstract: The effect of nicotine and diphenylamine on astaxanthin biosynthesis in Xanthophyllomyces dendrorhous was studied. The effects were analysed under standard and low temperature conditions. It was found that 10 mM-nicotine inhibits the cyclization of lycopene and de novo protein synthesis was not needed to reverse the inhibition. The oxidation of beta-carotene was irreversibly inhibited by 10 microM-diphenylamine while the dehydrogenation of phytoene was reversibly inhibited by 60 microM-diphenylamine. The simultaneous exposure to low temperature (4 degrees C) overcomes the inhibition of beta-carotene oxidation at low diphenylamine concentration.
authors.affiliation: Institut für Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Jülich, Germany.
authors.forename: L M
authors.initials: LM
authors.lastname: Ducrey Sanpietro
authors.forename: M R
authors.initials: MR
authors.lastname: Kula
mesh_terms: D000975:Antioxidants; D001487:Basidiomycota / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004159:Diphenylamine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009538:Nicotine / Q000494:pharmacology*; D013696:Temperature; D013997:Time Factors; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000975:Antioxidants; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; D009538:Nicotine; C100185:(all-E) phytoene; C005948:astaxanthine; D003513:Cycloheximide; D004159:Diphenylamine