[Analysis of contact dermatitis by cytokines. III.--Sensitization and crossreaction of phenolic compounds].
We examined sensitization and crossreaction by the guinea pig maximization test (GPMT) with phenolic compounds. Skin specimens were collected from earlobes of BALB/c mice on 24 h after elicitation phase of contact hypersensitivity reaction (CHR) with phenolic compounds. The expression of cytokines of skin specimens was examined by the reverse transcriptase/polymerase chain reaction (RT-PCR) method. Consequently, phenolic compounds which showed positive reaction in GPMT expressed IL-2 and IFN-gamma on 24 h after elicitation, and some phenolic compounds showed marked crossreaction. Therefore, it was found that on several phenolic compounds, dimerization of these compounds from monomer to dimer decrease sensitization.
Yamazaki
S
S
Kanebo, Ltd., Cosmetics Laboratory, Kanagawa, Japan.
Suzuki
K
K
Ikemoto
T
T
Kakishima
H
H
D000818:Animals; D003429:Cross Reactions; D016207:Cytokines / Q000032:analysis / Q000378:metabolism*; D003877:Dermatitis, Contact / Q000276:immunology*; D019281:Dimerization; D005054:Eugenol / Q000031:analogs & derivatives* / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D051379:Mice; D008807:Mice, Inbred BALB C; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology*; D012882:Skin Tests / Q000379:methods
D016207:Cytokines; D005054:Eugenol; C036643:isoeugenol