[The transfusion aspects of allogeneic bone marrow transplantation].
OBJECTIVE
Analysis of transplantations of allogenic bone marrow (BM) for determination of transfusiological problems arising in various types of donor's and recipient's blood ABO incompatibility.


METHODS
46 allogenic BM transplantations from relative donors are analysed. The following types of ABO incompatibility were identified: significant (12 donor-recipient pairs), insignificant (10 pairs), combined (2 pairs).


RESULTS
The proposed methods of BM fractionation provided the yield of nucleated cells in minimal admixture of red cells. This allowed to minimize loss of stem cells at fractionation and to prevent acute hemolysis at infusion of BM suspension in cases of ABO group-different BM allotransplantations.


CONCLUSIONS
The proposed modification of the "shelf" method enabled effective fractionation of small BM volumes obtained from children-BM donors with high yield of nucleated cells and small admixture of incompatible red cells. Reinfusion of erythrocytic mass obtained at fractionation of donor BM fractionation used as replacement therapy makes allogenic blood transfusion unnecessary in 66% of BM donors.
Fregatova
L M
LM
Volkova
O Ia
OIa
Shavva
S A
SA
Platonova
G G
GG
Afanas'ev
B V
BV
D000017:ABO Blood-Group System / Q000276:immunology; D016026:Bone Marrow Transplantation / Q000276:immunology / Q000379:methods; D002648:Child; D017707:Erythrocyte Transfusion / Q000379:methods; D006680:HLA Antigens / Q000276:immunology; D006461:Hemolysis / Q000276:immunology; D006650:Histocompatibility Testing; D006801:Humans; D014184:Transplantation, Homologous
D000017:ABO Blood-Group System; D006680:HLA Antigens