title: Pathogenicity of an enterotoxigenic Escherichia coli hemolysin (hlyA) mutant in gnotobiotic piglets.
abstract: Pigs infected with hemolytic F4(+) strains of enterotoxigenic Escherichia coli often develop septicemia secondary to intestinal infection. We tested the hypothesis that inactivation of hemolysin would reduce the ability of F4(+) enterotoxigenic E. coli to cause septicemia in swine following oral inoculation. Inactivation of the hemolysin structural gene (hlyA) did not decrease the incidence of septicemia in the gnotobiotic piglet model.
authors.affiliation: Departments of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska 68583, USA. vets044@unlvm.unl.edu
authors.forename: R A
authors.initials: RA
authors.lastname: Moxley
authors.forename: E M
authors.initials: EM
authors.lastname: Berberov
authors.forename: D H
authors.initials: DH
authors.lastname: Francis
authors.forename: J
authors.initials: J
authors.lastname: Xing
authors.forename: M
authors.initials: M
authors.lastname: Moayeri
authors.forename: R A
authors.initials: RA
authors.lastname: Welch
authors.forename: D R
authors.initials: DR
authors.lastname: Baker
authors.forename: R G
authors.initials: RG
authors.lastname: Barletta
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000662:veterinary; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000096:biosynthesis; D004768:Enterotoxins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000662:veterinary*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D005856:Germ-Free Life; D006460:Hemolysin Proteins / Q000235:genetics*; D007583:Jejunum / Q000382:microbiology; D009154:Mutation; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; C036507:Hlya protein, E coli