Molecular detection and quantitation of the chemokine RANTES mRNA in neurological brain.
Accumulation of T-cells in the brains of patients with neurological disorders prompted a molecular analysis of brain tissue for expression of the chemokine RANTES, which is known to be a T-cell activator and chemoattractant. A fast, sensitive and reproducible technique was developed, based on the polymerase chain reaction and nonradioactive detection. The method could detect and quantitate RANTES in small amounts of brain tissue from all patients with multiple sclerosis, and in some patients with other neural or inflammatory diseases. The data indicate constitutive expression of RANTES in brain from some neurological disorders where its downregulation can have therapeutic benefits.
Hvas
J
J
Department of Medical Microbiology and Immunology, University of Aarhus, Denmark.
Bernard
C C
CC
D000368:Aged; D001923:Brain Chemistry; D001927:Brain Diseases / Q000235:genetics* / Q000378:metabolism; D001932:Brain Neoplasms / Q000235:genetics / Q000378:metabolism; D018946:Chemokine CCL5 / Q000235:genetics* / Q000592:standards; D017931:DNA Primers; D006801:Humans; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D018946:Chemokine CCL5; D017931:DNA Primers; D012333:RNA, Messenger