Structure of the Na+-driven flagellum from the homoacetogenic bacterium Acetobacterium woodii.
The Na+-dependent flagellum of Acetobacterium woodii was characterised. Flagellin and whole flagella were purified and analysed by SDS-PAGE and electron microscopy. The structure and dimensions of the filament and the hook-basal body, as revealed by electron microscopy, resemble those of H+-dependent flagella from gram-positive bacteria. Intramembrane particle rings were present at the cell pole in freeze-fractured A. woodii cells, which might correspond to the mot complex.
Aufurth
S
S
Lehrstuhl für Mikrobiologie der Ludwig-Maximilians-Universität München, Munich, Germany.
Madkour
M
M
Mayer
F
F
Müller
V
V
D001426:Bacterial Proteins / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005407:Flagella / Q000737:chemistry / Q000648:ultrastructure*; D005614:Freeze Fracturing; D016987:Gram-Positive Rods / Q000737:chemistry / Q000648:ultrastructure; D008854:Microscopy, Electron; D012964:Sodium / Q000378:metabolism*
D001426:Bacterial Proteins; D012964:Sodium