title: Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis.
abstract: The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair magnitude of delta bp/Ave.-bp) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.
authors.affiliation: Department of Chemistry, Wichita State University, Kansas 67260-0051, USA.
authors.forename: O
authors.initials: O
authors.lastname: Otim
authors.forename: R P
authors.initials: RP
authors.lastname: Singhal
mesh_terms: D004279:DNA, Viral / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D010957:Plasmids / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral