Stabilization of Tc-99m D,L-HMPAO preparations as a leucocyte labelling agent.
An attempt was made to use stabilized Tc-99m D,L-HMPAO (S-HMPAO) to label leucocytes. The radiochemical purity of Tc-99m D,L-HMPAO, labelling efficiency of leucocytes, cell viability of labelled leucocytes, and stability of S-HMPAO labelled leucocytes were calculated. In comparison with commercial Tc-99m D,L-HMPAO (C-HMPAO) without stabilization, immediately, at 0.5, 1, 2, 4, and 6 h after HMPAO preparation, the radiochemical purity of S-HMPAO and the labelling efficiencies of S-HMPAO labelled leucocytes were higher. S-HMPAO is more stable than C-HMPAO and can provide higher leucocyte labelling efficiency. S-HMPAO, therefore, has the potential to replace C-HMPAO as a leucocyte-labelling agent.
Kao
C H
CH
Department of Nuclear Medicine, Taichung Veterans General Hospital, Taiwan, ROC.
Huang
W T
WT
Tsai
C S
CS
D002470:Cell Survival; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D019690:Technetium Tc 99m Exametazime / Q000097:blood* / Q000737:chemistry*
D019690:Technetium Tc 99m Exametazime