title: Effects of dialysis membranes on the kinetics of tumor necrosis factor-alpha production by peripheral mononuclear cells in chronic hemodialysis patients.
abstract: To evaluate the biocompatibility of dialysis membranes, blood samples were collected from 10 hemodialysis patients immediately before dialysis and peripheral blood mononuclear cells were isolated. The 3.0 x 10(5) cells/ml were then passed 30 times through modules made of a polyethylene glycol-grafted cellulose membrane, a polyacrylonitrile membrane, and a polysulfone membrane. Expression of messenger RNA for tumor necrosi factor-alpha (TNF-alpha) was determined. Cells were also cultured for 2 h with and without lipopolysaccharide and TNF-alpha levels in the supernatant were measured. TNF-alpha messenger RNA expression was significantly higher immediately after passage through the polyacrylonitrile membrane compared with the other membranes. Cells cultured without lipopolysaccharide, produced significantly less TNF-alpha after passage through the polysulfone membrane, while lipopolysaccharide significantly increased TNF-alpha production by cells passed through the polyacrylonitrile membrane. These results suggest that biocompatibility differs even among dialysis membranes believed to cause no complement activation.
authors.affiliation: Second Department of Internal Medicine, Hiroshima University School of Medicine, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kushihata
authors.forename: N
authors.initials: N
authors.lastname: Yorioka
authors.forename: H
authors.initials: H
authors.lastname: Oda
authors.forename: X F
authors.initials: XF
authors.lastname: Ye
authors.forename: M
authors.initials: M
authors.lastname: Yamakido
mesh_terms: D000180:Acrylic Resins; D000328:Adult; D001672:Biocompatible Materials; D003167:Complement Activation; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008070:Lipopolysaccharides; D008297:Male; D008567:Membranes, Artificial; D008875:Middle Aged; D011092:Polyethylene Glycols; D011108:Polymers; D012333:RNA, Messenger / Q000378:metabolism; D006435:Renal Dialysis; D013450:Sulfones; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D008070:Lipopolysaccharides; D008567:Membranes, Artificial; D011108:Polymers; D012333:RNA, Messenger; D013450:Sulfones; D014409:Tumor Necrosis Factor-alpha; C010504:polyacrylonitrile; C017662:polysulfone P 1700; D011092:Polyethylene Glycols