Induction of apoptosis by Chlamydia psittaci and Chlamydia trachomatis infection in tissue culture cells.
The role of programmed cell death (apoptosis) in LLC-MK2 cells infected with Chlamydia trachomatis LGV2 serotype and Chlamydia psittaci 6BC strain was investigated using flow cytometry and TUNEL procedures. The number of apoptotic cells was significantly higher at 72 and 96 hours post infection in the Chlamydia infected cell cultures in comparison with mock-infected cells. We postulate the apoptotic process to be a mechanism induced by C. trachomatis and C. psittaci infection in LLC-MK2 cells.
Gibellini
D
D
Department of Clinical and Experimental Medicine-Microbiology Section, S. Orsola General Hospital, University of Bologna, Italy.
Panaya
R
R
Rumpianesi
F
F
D000818:Animals; D017209:Apoptosis; D002453:Cell Cycle; D002460:Cell Line; D002690:Chlamydia Infections / Q000473:pathology*; D002692:Chlamydia trachomatis / Q000254:growth & development / Q000472:pathogenicity*; D002691:Chlamydophila psittaci / Q000254:growth & development / Q000472:pathogenicity*; D005434:Flow Cytometry; D020287:In Situ Nick-End Labeling / Q000379:methods; D007668:Kidney / Q000166:cytology; D008253:Macaca mulatta; D018613:Microscopy, Confocal