title: Prevalence of Lithuanian mutation among St. Petersburg Jews with familial hypercholesterolemia.
abstract: We used polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis to detect LDL receptor gene defects in the St. Petersburg population. We have found a deltaG197 mutation in several patients of Jewish origin. The mutation named is shown to be responsible for one-third (7/23) of familial hypercholesterolemia (FH) cases in St. Petersburg Jews and absent in patients of Russian descent. The prevalence of a deltaG197 mutation in St. Petersburg Jews is consistent with its origin in Lithuania or Poland. The deltaG197 mutation can be easily detected in polyacrylamide minigels because of formation of specific heteroduplexes during PCR with DNA of heterozygous patients. Taken together with high prevalence of the mutation in St. Petersburg Jews, this observation provides an opportunity for DNA diagnostics of FH in this ethnic group.
authors.affiliation: Institute for Experimental Medicine, St. Petersburg, Russia.
authors.forename: M
authors.initials: M
authors.lastname: Mandelshtam
authors.forename: K
authors.initials: K
authors.lastname: Chakir
authors.forename: S
authors.initials: S
authors.lastname: Shevtsov
authors.forename: V
authors.initials: V
authors.lastname: Golubkov
authors.forename: N
authors.initials: N
authors.lastname: Skobeleva
authors.forename: B
authors.initials: B
authors.lastname: Lipovetsky
authors.forename: V
authors.initials: V
authors.lastname: Konstantinov
authors.forename: A
authors.initials: A
authors.lastname: Denisenko
authors.forename: V
authors.initials: V
authors.lastname: Gaitskhoki
authors.forename: E
authors.initials: E
authors.lastname: Schwartz
mesh_terms: D004252:DNA Mutational Analysis; D005260:Female; D006580:Genetic Carrier Screening; D020180:Heteroduplex Analysis; D006801:Humans; D006938:Hyperlipoproteinemia Type II / Q000097:blood / Q000208:ethnology / Q000235:genetics*; D007585:Jews / Q000235:genetics*; D008055:Lipids / Q000097:blood; D008097:Lithuania; D008297:Male; D010375:Pedigree; D018807:Polymorphism, Single-Stranded Conformational; D012426:Russia; D017384:Sequence Deletion / Q000235:genetics*; D044465:White People / Q000235:genetics
chemical_list: D008055:Lipids