title: Osteoinduction using bone morphogenic protein in irradiated tissue.
abstract: OBJECTIVE
  To prove the efficacy of bone morphogenic protein as an osteoinductive agent in irradiated tissue.
  
  
  METHODS
  Prospective randomized controlled trial designed to test the effectiveness of recombinant bone morphogenic protein 2 (rBMP-2) combined with solid hydroxyapatite disks in an irradiated tissue bed.
  
  
  METHODS
  Eighteen adult, male, white New Zealand rabbits weighing 3.0 to 3.5 kg.
  
  
  METHODS
  The rabbits were randomly divided, with 9 receiving radiation treatment and 9 receiving no radiation treatment. Each animal underwent implantation of 2 hydroxyapatite disks onto the snout at 9 weeks following radiation treatment. One disk was impregnated with rBMP-2 and the other with buffer only. The animals were killed at 3, 6, or 20 weeks after implantation for analysis.
  
  
  RESULTS
  Histological analysis demonstrated that rBMP-2 was equally effective as an osteoinductive agent in the irradiated and nonirradiated tissue. We also found significantly increased new bone formation in the rBMP-2 group vs the buffer group.
  
  
  CONCLUSIONS
  This study supports the potential clinical utility of rBMP-2 and solid hydroxyapatite in irradiated tissue beds. These findings have interesting implications for patients with head and neck cancer who have undergone radiation therapy and need bony reconstruction.
authors.affiliation: Department of Otorhinolaryngology, University of Texas Southwestern Medical Center, Dallas 75235-9035, USA.
authors.forename: B K
authors.initials: BK
authors.lastname: Howard
authors.forename: K R
authors.initials: KR
authors.lastname: Brown
authors.forename: J L
authors.initials: JL
authors.lastname: Leach
authors.forename: C H
authors.initials: CH
authors.lastname: Chang
authors.forename: D I
authors.initials: DI
authors.lastname: Rosenthal
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001842:Bone and Bones / Q000528:radiation effects; D017886:Durapatite; D020007:Implants, Experimental; D008297:Male; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D011817:Rabbits; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D017886:Durapatite