Aseptic laparoscopic colon resection with intraabdominal anastomosis. An experimental study in pigs.
BACKGROUND
We evaluated a new aseptic method for laparoscopic left colon resection in terms of technical feasibility and outcome.


METHODS
Ten pigs were operated on under general anesthesia. Pre- and postoperative body weight, stools, behavior, and need for analgesics were recorded. Fourteen days later, the animals were killed. At autopsy, the degree of intraabdominal adhesions was noted. The anastomoses were sent for histological examination. The entire procedure was performed intracorporeally, and no antibiotics were given. After division of the mesocolon, the segment to be resected was invaginated down through the colon. This was facilitated by a custom-made instrument that was introduced into the bowel via the anus; it consisted of a pull-out device and a modified diathermy wire. The anastomosis was completed at the invagination fold by a row of hernia staples that were covered by an interrupted suture. Then the invaginated bowel was transected by the diathermy wire and delivered through the anus.


RESULTS
One animal was killed before completion of the operation because of a colonic perforation. The remaining nine animals had an uneventful and rapid recovery. They ate from the 1st postoperative day and gained weight rapidly. Stools were normal after 2 days (median), and normal behaviour was noted in all animals from the 1st postoperative day. At the postmortem examination, intraabdominal adhesions were observed in two animals. In one case, the adhesions extended from a hematoma in the mesentery to the abdominal wall. There were no adhesions to the anastomosis or the colon. In the other case, the anastomosis adhered to the right uterine tube and a loop of small intestines.


CONCLUSIONS
The method is technically feasible, but a modification is suggested for cases where the invagination is impossible. Recovery after the operation is rapid.
Jørgensen
L S
LS
Department of Surgical Gastroenterology, Aarhus Kommunehospital, Aarhus University Hospital, DK-8000 Aarhus C, Denmark.
Langkilde
N C
NC
Møller
P K
PK
Mortensen
F V
FV
Tei
T M
TM
Jacobsen
N O
NO
D000714:Anastomosis, Surgical / Q000009:adverse effects / Q000379:methods*; D000818:Animals; D001207:Asepsis; D003082:Colectomy / Q000379:methods*; D003106:Colon / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D005260:Female; D010535:Laparoscopy / Q000009:adverse effects / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine