Role of CD86 (B7-2) in triggering of antigen-specific IgE antibody response by lipopolysaccharide.
The role of CD86 in triggering of ascaris extract-specific IgE antibody response by lipopolysaccharide was studied. The simultaneous administration of anti-CD86 antibody with ascaris extract and lipopolysaccharide prevented the production of IgE antibody response to ascaris extract. CD86+ cells were detected in peritoneal cavities and spleens of mice injected intraperitoneally with lipopolysaccharide. CD86+ cells appeared in peritoneal cavities and spleens eight hours after lipopolysaccharide injection, and they were detectable for a week. CD86+ cells in peritoneal cavities and spleens were mainly surface Ig-positive B-cells and some Ig-negative cells. It was suggested that lipopolysaccharide induced the expression of CD86 mainly on B-cells, and that CD86+ cells induced by lipopolysaccharide injection might play an important role as antigen-presenting cells on triggering of ascaris extract-specific IgE antibody response by lipopolysaccharide.
Jiang
G Z
GZ
Department of Microbiology and Immunology, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Japan.
Kato
Y
Y
Sugiyama
T
T
Koide
N
N
Chakravortty
D
D
Kawai
M
M
Fukada
M
M
Yoshida
T
T
Yokochi
T
T
D000069594:Abatacept; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis; D015703:Antigens, CD / Q000276:immunology*; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001200:Ascaris / Q000276:immunology; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; D004305:Dose-Response Relationship, Drug; D018796:Immunoconjugates; D007073:Immunoglobulin E / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008070:Lipopolysaccharides / Q000276:immunology; D008221:Lymphoid Tissue; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010537:Peritoneum / Q000276:immunology; D051381:Rats; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000943:Antigens, Differentiation; D051940:B7-2 Antigen; D060908:CTLA-4 Antigen; C497965:Cd86 protein, mouse; C497966:Cd86 protein, rat; C556707:Ctla4 protein, mouse; C556708:Ctla4 protein, rat; D018796:Immunoconjugates; D008070:Lipopolysaccharides; D008562:Membrane Glycoproteins; D007073:Immunoglobulin E; D000069594:Abatacept