Identification of a gene encoding a methyl-accepting chemotaxis-like protein form Campylobacter coli and its use in a molecular typing scheme for campylobacters.
Using PCR amplification with degenerate primers, a gene (tlpA) form Campylobacter coli encoding a putative 63x0 kDa polypeptide which exhibited significant identity with bacterial methyl-accepting chemotaxis proteins (MCPs) was identified. A mutant containing an inactivated copy of the tlpA A gene showed a wild-type chemotactic response to all of the chemo-attractants tested. A DNA probe based on the Highly Conserved Domain (HCD) of TlpA revealed the presence of multiple copies of genes encoding MCP-like proteins in both Camp. coli and Camp. jejuni. The arrangement of restriction sites within, and proximal to, genes with homology to the HCD probe varied among strains, resulting in a high degree of polymorphism. It is demonstrated here that a DNA probe compromising the HCD region of MCP-like proteins can be used, in Southern hybridization-based assays, to provide novel information which allows the discrimination of individual strains of Camp. coli and Camp. jejuni.
Gonzalez
I
I
Institute of Food Research, Reading Laboratory, UK.
Richardson
P T
PT
Collins
M D
MD
Park
S F
SF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification; D017000:Campylobacter coli / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008565:Membrane Proteins / Q000235:genetics*; D000072236:Methyl-Accepting Chemotaxis Proteins; D008969:Molecular Sequence Data; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D017931:DNA Primers; D008565:Membrane Proteins; D000072236:Methyl-Accepting Chemotaxis Proteins