title: JC9813-A putative novel human papillomavirus identified by PCR-DS.
abstract: Papillomaviruses consist of more than 130 viral types described so far. Most of them are human papillomaviruses (HPV) of supergroup A, demonstrating ano-genital tropism and characterized as etiological agents for benign and malignant cervical lesions in women. A PCR-direct sequencing (PCR-DS) approach with P-33 labeled dideoxynucleotides was used to detect and type human papillomaviruses in cervical biopsies. One novel sequence was identified in a LSIL (low-grade squamous intraepithelial lesions) specimen from an HIV-positive English Canadian patient. The structure of the viral gene L1 was determined, yielding a putative novel HPV type of supergroup A (clade A8) named JC9813.
authors.affiliation: Hôpital Ste-Justine, Université de Montréal, Canada.
authors.forename: J C
authors.initials: JC
authors.lastname: Feoli-Fonseca
authors.forename: L L
authors.initials: LL
authors.lastname: Oligny
authors.forename: M
authors.initials: M
authors.lastname: Filion
authors.forename: P
authors.initials: P
authors.lastname: Simard
authors.forename: P A
authors.initials: PA
authors.lastname: Russo
authors.forename: W V
authors.initials: WV
authors.lastname: Yotov
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D001706:Biopsy; D036022:Capsid Proteins; D004279:DNA, Viral; D005260:Female; D015658:HIV Infections / Q000150:complications; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009856:Oncogene Proteins, Viral / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D014412:Tumor Virus Infections / Q000150:complications / Q000473:pathology
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; C114580:HPV L1 protein, Human papillomavirus; D009856:Oncogene Proteins, Viral