Modification of organ protein synthesis after surgical stress by low energy diets with different supplements.
We have studied the effects of hypocaloric diets with different supplements on liver and jejunal mucosa protein synthesis. The supplements assayed were medium chain triglycerides (diet MCT, with 50% carbohydrates: 25% long chain triglycerides (LCT): 25% medium chain triglycerides (MCT), standard amino acids), branched-chain amino acids (diet BCA, identical to control diet L50, with 15.3% of nitrogen replaced by branched-chain amino acids) and glutamine (diet GLN, identical to diet L50, with 15.3% of nitrogen replaced by glutamine). The control diet (L50) had 50% carbohydrates: 50% LCT and standard amino acids. The diets were assayed on 86 rats with femoral fracture immobilized by Kirschner pin insertion. Nutrition was administered for 4 days. On the fifth day, liver and jejunal mucosa protein synthesis was determined. A branched-chain amino acid supply in a proportion higher than 21.2% of amino acid nitrogen significantly decreased liver and jejunal mucosa protein synthesis, while the same amount of glutamine did not modify it. MCT had no effect on jejunal mucosa protein synthesis, while it was decreased significantly in the liver.
Schwartz
S
S
Centre for Research in Biochemistry and Molecular Biology, Hospitals Vall d'Hebron, Barcelona, Spain.
López-Hellin
J
J
Garcia-Arumi
E
E
Arbos
M A
MA
Andreu
A L
AL
Farriol
M
M
D000597:Amino Acids, Branched-Chain / Q000008:administration & dosage; D000818:Animals; D001284:Atrophy; D002241:Carbohydrates / Q000008:administration & dosage; D002149:Energy Intake; D005264:Femoral Fractures / Q000601:surgery; D005973:Glutamine / Q000008:administration & dosage; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000473:pathology; D007583:Jejunum / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D010288:Parenteral Nutrition; D011182:Postoperative Care; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological; D014280:Triglycerides / Q000008:administration & dosage
D000597:Amino Acids, Branched-Chain; D002241:Carbohydrates; D014280:Triglycerides; D005973:Glutamine