p53 exon 7 mutations as a predictor of poor prognosis in patients with colorectal cancer.
We have studied 61 resected colorectal adenocarcinomas in order to investigate p53 mutations as a prognostic factor for this pathology. Mutations in exons 5-9 of the p53 gene were analyzed by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique followed by sequencing. Our data indicate that p53 exon 7 mutations were prevalent in the latest stages of colorectal carcinogenesis and patients bearing this alteration had the worst prognosis. Therefore, according to our results, mutations affecting exon 7 of the p53 gene could be considered as a useful marker of biological aggressiveness for colorectal cancer.
Iniesta
P
P
Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Vega
F J
FJ
Caldés
T
T
Massa
M
M
de Juan
C
C
Cerdán
F J
FJ
Sánchez
A
A
López
J A
JA
Torres
A J
AJ
Balibrea
J L
JL
Benito
M
M
D000368:Aged; D000704:Analysis of Variance; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005091:Exons / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D016016:Proportional Hazards Models; D016019:Survival Analysis