T-Helper 1 and T-helper 2 cytokine responses in gut-associated lymphoid tissue following enteric reovirus infection.
Enteric infection of mice with reovirus serotype 1 elicits antibody and cytotoxic T-lymphocytes in gut-associated lymphoid tissue (GALT). This led to the hypothesis that T-helper 1 (Th1) and T-helper 2 (Th2) responses develop in GALT. Reverse transcriptase-polymerase chain reactions on RNA from Peyer's patches (PP), intraepithelial lymphocytes (IEL), and lamina propria (LP) lymphocytes demonstrated that interferon (IFN)-gamma message was increased in PP and IEL, but not in LP following infection. No increase in mRNA for interleukin (IL)-4, IL-5, or IL-6 was detected. IFN-gamma, IL-5, and IL-6 were produced in in vitro cultures of PP 4-10 days postinfection. PP and spleen lymphocytes from infected mice produced IFN-gamma, but no IL-5 following in vitro restimulation. Infection also induced production of mRNA for the beta2 chain of the IL-12 receptor in PP. We conclude that reovirus induces robust Th1 and weak Th2 cell responses in GALT.
Fan
J Y
JY
Department of Microbiology and Immunology, Robert C. Byrd Health Sciences Center of West Virginia University, Morgantown, West Virginia 26506-9177, USA.
Boyce
C S
CS
Cuff
C F
CF
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D004751:Enteritis / Q000821:virology*; D007371:Interferon-gamma / Q000235:genetics; D007413:Intestinal Mucosa / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology* / Q000821:virology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010581:Peyer's Patches / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D012192:Retroviridae Infections / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D014779:Virus Replication
D016207:Cytokines; D012333:RNA, Messenger; D007371:Interferon-gamma