title: The primary structure of caprine PP3: amino acid sequence, phosphorylation, and glycosylation of component PP3 from the proteose-peptone fraction of caprine milk.
abstract: Proteose-peptone component 3 is a phosphorylated glycoprotein that was isolated from the proteose-peptone fraction of caprine milk. By mass spectrometric analysis, amino acid sequencing, and polymerase chain reaction analysis, the primary structure has been determined and has been shown to contain 136 amino acids. Phosphorylations were identified at Ser30 and Ser41. A partial glycosylation was present at Thr16, and a N-linked glycosylation was present at Asn78. Galactosamine was the amino sugar detected at Thr16. Glucosamine and galactosamine were the amino sugars found in the carbohydrate group linked to Asn78. The caprine amino acid sequence exhibits 88% identity with the bovine proteose-peptone component 3 sequence. However, when compared with the bovine sequence, the caprine sequence contains an insertion of a serine residue at position 25.
authors.affiliation: Department of Molecular and Structural Biology, University of Aarhus, Denmark.
authors.forename: I M
authors.initials: IM
authors.lastname: Lister
authors.forename: L K
authors.initials: LK
authors.lastname: Rasmussen
authors.forename: L B
authors.initials: LB
authors.lastname: Johnsen
authors.forename: L
authors.initials: L
authors.lastname: Møller
authors.forename: T E
authors.initials: TE
authors.lastname: Petersen
authors.forename: E S
authors.initials: ES
authors.lastname: Sørensen
mesh_terms: D000595:Amino Acid Sequence; D000606:Amino Sugars / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002364:Caseins / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D005260:Female; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D006041:Goats; D008892:Milk / Q000737:chemistry*; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000378:metabolism; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D017385:Sequence Homology
chemical_list: D000606:Amino Sugars; D002364:Caseins; D006023:Glycoproteins; C085522:PP3 protein, Bos taurus; D010446:Peptide Fragments; D010750:Phosphoproteins; D010768:Phosphoserine