Linkage mapping of fifty-eight new rat microsatellite markers.
Fifty-eight new anonymous simple sequence repeats (SSR) were generated and mapped to various rat chromosomes. Among them two genes (rat homologs for human cadherin-14 and mouse fibroblast growth factor-related protein) were mapped on Chromosomes (Chrs) 2 and 11 respectively. The majority of markers were generated from a small insert genomic library specific to Chr 11, 13, 14, and 15. Twenty new markers were mapped to Chr 13, which is known to contain a blood pressure quantitative trait locus (QTL). Several approaches to obtain microsatellite markers are described. The protocols and newly generated markers should be useful for ongoing rat genome project.
Sverdlov
V E
VE
Department of Physiology and Molecular Medicine, Medical College of Ohio, 3035 Arlington Avenue, Toledo, Ohio 43614-5804, USA.
Dukhanina
O I
OI
Hoebee
B
B
Rapp
J P
JP
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D008040:Genetic Linkage; D006801:Humans; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D051381:Rats / Q000235:genetics*; D011919:Rats, Inbred Strains
D017931:DNA Primers