title: Evaluation of the tacrolimus II microparticle enzyme immunoassay (MEIA II) in liver and renal transplant recipients.
abstract: We evaluated the MEIA II with blood samples with added tacrolimus (3.0, 5.0, 11.0, and 22.0 microg/L). The assay had acceptable recoveries (99-103%) and intraday imprecision (<16.0%) across the range of concentrations studied, except for the recoveries at 3.0 microg/L (86.3%) and 5.0 microg/L (80.7%). Comparison of liver (n = 116) and renal (n = 113) patient samples measured by MEIA II against HPLC-tandem mass spectrometry (HPLC-MS/MS) found a mean overestimation of 15.6%. From these comparison data it can be calculated that at values of 5 and 20 microLg/L in liver or renal transplant patient samples, measured by HPLC-MS/MS, MEIA II will have the corresponding range estimates of 3.6-7.9 microg/L and 20.9-25.4 microg/L, respectively. No clinically significant difference in results, in terms of overestimation or correlation, was observed between the two transplant groups studied. The MEIA II is an improvement on the previous MEIA I and is suitable for the therapeutic drug monitoring of tacrolimus where HPLC-MS/MS is unavailable.
authors.affiliation: The University of Queensland Department of Medicine, Princess Alexandra Hospital, Brisbane, Australia.
authors.forename: J L
authors.initials: JL
authors.lastname: Cogill
authors.forename: P J
authors.initials: PJ
authors.lastname: Taylor
authors.forename: I S
authors.initials: IS
authors.lastname: Westley
authors.forename: R G
authors.initials: RG
authors.lastname: Morris
authors.forename: S V
authors.initials: SV
authors.lastname: Lynch
authors.forename: A G
authors.initials: AG
authors.lastname: Johnson
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007124:Immunoenzyme Techniques; D007166:Immunosuppressive Agents / Q000097:blood*; D016030:Kidney Transplantation; D016031:Liver Transplantation; D013058:Mass Spectrometry; D016559:Tacrolimus / Q000097:blood*
chemical_list: D007166:Immunosuppressive Agents; D016559:Tacrolimus