title: [Diagnosis of partial hydatidiform mole using molecular genetics].
abstract: The aim of our study was to confirm the diagnosis of partial hydatidiform mole (PMH) and to determine mechanism of PMH development by chromosomal DNA analysis. In our study we analysed 8 cases of morphologically and histologically confirmed PMH. Their karyotype was determined by using methods of direct, 24-hour and a long-term tissue culture. The restriction fragment length polymorphisms (RFLP) method was used for DNA analysis of PMH chorionic villi and from progenitor lymphocytes in peripheral blood. All cases were triploid. DNA analysis revealed in six cases one maternal and two paternal RFLP bands, and in two cases two maternal and one paternal RFLP bands.
authors.affiliation: Ustav lekárskej biológie LF UK, Bratislava.
authors.forename: V
authors.initials: V
authors.lastname: Repiská
authors.forename: J
authors.initials: J
authors.lastname: Vojtassák
authors.forename: M
authors.initials: M
authors.lastname: Korbel'
authors.forename: D
authors.initials: D
authors.lastname: Böhmer
authors.forename: J
authors.initials: J
authors.lastname: Malová
authors.forename: L
authors.initials: L
authors.lastname: Demjénová
authors.forename: V
authors.initials: V
authors.lastname: Zajac
mesh_terms: D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000175:diagnosis* / Q000235:genetics; D007621:Karyotyping; D008967:Molecular Biology; D012150:Polymorphism, Restriction Fragment Length; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000175:diagnosis* / Q000235:genetics
chemical_list: D004273:DNA, Neoplasm