title: Further purification and structural analysis of calcium spirulan from Spirulina platensis.
abstract: An antiviral sulfated polysaccharide, calcium spirulan (Ca-SP), isolated from Spirulina platensis, was subjected to further purification. Ca-SP was found to be composed of rhamnose, 3-O-methylrhamnose (acofriose), 2,3-di-O-methylrhamnose, 3-O-methylxylose, uronic acids, and sulfate. The backbone of Ca-SP consisted of 1,3-linked rhamnose and 1,2-linked 3-O-methylrhamnose units with some sulfate substitution at the 4-position. The polymer was terminated at the nonreducing end by 2,3-di-O-methylrhamnose and 3-O-methylxylose residues.
authors.affiliation: Faculty of Pharmaceutical Sciences and School of Medicine, Toyama Medical and Pharmaceutical University, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: J B
authors.initials: JB
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Hayashi
authors.forename: K
authors.initials: K
authors.lastname: Hayashi
authors.forename: U
authors.initials: U
authors.lastname: Sankawa
authors.forename: M
authors.initials: M
authors.lastname: Maeda
authors.forename: T
authors.initials: T
authors.lastname: Nemoto
authors.forename: H
authors.initials: H
authors.lastname: Nakanishi
mesh_terms: D000998:Antiviral Agents / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology; D002240:Carbohydrate Sequence; D002460:Cell Line; D002848:Chromatography, DEAE-Cellulose; D000458:Cyanobacteria / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D018259:Herpesvirus 1, Human / Q000187:drug effects; D004854:Herpesvirus 4, Human; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011134:Polysaccharides / Q000032:analysis / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D011134:Polysaccharides; C102617:spirulan