Reverse transcriptase-polymerase chain reaction study of anion exchanger-2 in canine tissues and different Madin-Darby canine kidney cell types.
Reverse transcriptase-polymerase chain reaction (RT-PCR) of mRNA from canine large intestine, skeletal muscle, pancreas, kidney, and spleen and from cultured wild-type and C7 and C11 Madin-Darby canine kidney (MDCK) cells revealed considerable variation in anion exchanger (AE)1 and AE2 mRNA levels between the tissues. Similar high levels of AE2 mRNA were detected in all the MDCK cell populations. AE2 in MDCK cells is probably the basolateral Cl-/HCO3- exchanger common to the principal and beta-intercalated cells.
Prime
G
G
Institute of Physiology, University of Munich, Germany. G.PRIME@lrz.uni-muenchen.de
Ebner
S
S
Marin-Grez
M
M
D000818:Animals; D027321:Anion Transport Proteins; D017920:Antiporters; D015152:Blotting, Northern; D002460:Cell Line; D004076:Digoxigenin; D004285:Dogs; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D007685:Kidney Tubules, Collecting / Q000737:chemistry* / Q000201:enzymology; D008565:Membrane Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D064606:SLC4A Proteins
D027321:Anion Transport Proteins; D017920:Antiporters; D008565:Membrane Proteins; D012333:RNA, Messenger; D064606:SLC4A Proteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D004076:Digoxigenin