Detection of the Ki-67 proliferation associated nuclear epitope in normal canine tissues using the monoclonal antibody MIB-1.
This report describes the immunohistochemical detection of the Ki-67 proliferation associated nuclear epitope by means of the MIB-1 monoclonal antibody (mAb) in paraffin tissue sections from nine samples of 16 tissues obtained from nine dogs. Three parameters were considered: the localization of the cells expressing the Ki-67 epitope, the cytological characteristics of the Ki-67 expression, and the proliferative activity of some of the tissues measured by means of the proliferative index (percentage of Ki-67 positive cells measured on 500 and 1000 cells). The MIB-1 mAb reacts with the nuclei of proliferating cells, as in humans. The proliferative index was far from representative, but we were able to give a range of values concerning the proliferative activity of normal tissues. This study serves as a basis for the expression of the Ki-67 antigen by normal canine tissues in order to visualize the proliferative compartments and, thus, allows its application as a proliferative marker in routine veterinarian histopathology.
Laprie
C
C
Unité d'Anatomie pathologique, Ecole Nationale Vétérinaire de Toulouse, France.
Abadie
J
J
Amardeilh
M F
MF
Raymond
I
I
Delverdier
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology; D002455:Cell Division; D004285:Dogs / Q000254:growth & development* / Q000276:immunology; D000939:Epitopes / Q000032:analysis / Q000276:immunology; D005260:Female; D005753:Gastric Mucosa / Q000166:cytology / Q000276:immunology; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology; D019394:Ki-67 Antigen / Q000032:analysis* / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008297:Male; D012867:Skin / Q000166:cytology / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000939:Epitopes; D019394:Ki-67 Antigen