Differential transferrin receptor density in human colorectal cancer: A potential probe for diagnosis and therapy.
Transferrin receptor density was investigated in human colorectal surgical specimens. Crude membranes were prepared from 23 cancer tumors (adenocarcinoma or malignant villous tumor) and 3 non-cancer tumors (polyadenoma or villous tumor) and 26 adjacent control mucosa. Contrary to non-cancer tumors, Scatchard analysis of 125I-transferrin binding data evidenced higher maximal transferrin binding capacity and lower dissociation constant in cancer tissues (Bmax cancer 1.828+/-0.320 nmol/g, Kd 24.1+/-4.7 nM), as compared to paired control colonic mucosa (Bmax contol 0.851+/-0.182 nmol/g, Kd 30.7+/-7.3 nM), paired t-tests: Bmax p<0.001, Kd p<0.05). As the cancer/control Bmax ratio was 2.6+/-0.4,transferrin carrier constructs should be proposed for cancer imaging or therapy.
Prost
A C
AC
Service Central de Medecine Nucleaire, Centre Hospitalier Le Mans, 72037 Le-Mans, France.
Ménégaux
F
F
Langlois
P
P
Vidal
J M
JM
Koulibaly
M
M
Jost
J L
JL
Duron
J J
JJ
Chigot
J P
JP
Vayre
P
P
Aurengo
A
A
Legrand
J C
JC
Rosselin
G
G
Gespach
C
C
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D011485:Protein Binding; D011869:Radioligand Assay; D011990:Receptors, Transferrin / Q000737:chemistry / Q000378:metabolism*; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism
D015415:Biomarkers; D011990:Receptors, Transferrin; D014168:Transferrin