Isolation and purification of immunoglobulins from chicken eggs using thiophilic interaction chromatography.
We report on the use of thiophilic interaction chromatography for the purification of IgY from egg yolk. This procedure permits the purification to homogeneity of IgY in a single chromatographic step after ammonium sulfate fractication. This study also compares the use of an improved T-gel which has a higher capacity for immunoglobulin than the original T-gel, having a capacity in excess of 25 mg IgY/ml resin. The recovery from this procedure is close to 100%, providing a simple and efficient means for purifying IgY from egg yolk. We also determined that the amount of specific antibody present in egg yolk from an immunised chicken is around 1% of total IgY.
Hansen
P
P
School of Biochemistry, La Trobe University, Bundoora, Victoria, Australia.
Scoble
J A
JA
Hanson
B
B
Hoogenraad
N J
NJ
D000818:Animals; D000918:Antibody Specificity; D002645:Chickens; D002850:Chromatography, Gel / Q000379:methods; D004527:Egg Proteins / Q000276:immunology / Q000302:isolation & purification; D004531:Eggs / Q000032:analysis*; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D008565:Membrane Proteins / Q000276:immunology / Q000494:pharmacology; D008623:Mercaptoethanol; D013450:Sulfones
D004527:Egg Proteins; C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008565:Membrane Proteins; D013450:Sulfones; D008623:Mercaptoethanol