Evaluation of focal cerebral ischemia in rats by magnetic resonance imaging and immunohistochemical analyses.
Correlation of focal ischemia-induced brain damage evidenced by magnetic resonance imaging (MRI) and by staining with microtubule-associated protein 2 (MAP2) was studied in rats. Ischemia was produced by transient occlusion of the middle cerebral artery (MCAO). The damage was assessed at 6 to 8 hours after MCAO and 1 week later. The area of damage assessed by MRI agreed with that by MAP2 staining at 6 to 8 hours after MCAO, which was smaller (P < 0.001) than that defined by MAP2 staining 1 week after MCAO. Glial staining indicated that glial infiltration affected the signal intensity of MRI in the area of damage.
Ishii
H
H
Department of Anesthesia, Kyoto University Hospital, Japan.
Arai
T
T
Morikawa
S
S
Inubushi
T
T
Tooyama
I
I
Kimura
H
H
Mori
K
K
D000818:Animals; D001157:Arterial Occlusive Diseases / Q000175:diagnosis; D005069:Evaluation Studies as Topic; D006973:Hypertension / Q000473:pathology; D007150:Immunohistochemistry; D002546:Ischemic Attack, Transient / Q000175:diagnosis* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D008297:Male; D008869:Microtubule-Associated Proteins; D051381:Rats; D011918:Rats, Inbred SHR
D008869:Microtubule-Associated Proteins