Monitoring retroviral RNA dimerization in vivo via hammerhead ribozyme cleavage.
We have used a strategy for colocalization of Psi (Psi)-tethered ribozymes and targets to demonstrate that Psi sequences are capable of specific interaction in the cytoplasm of both packaging and nonpackaging cells. These results indicate that current in vitro dimerization models may have in vivo counterparts. The methodology used may be applied to further genetic analyses on Psi domain interactions in vivo.
Pal
B K
BK
Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, California 91010, USA.
Scherer
L
L
Zelby
L
L
Bertrand
E
E
Rossi
J J
JJ
D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D019281:Dimerization; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000502:physiology; D051379:Mice; D008969:Molecular Sequence Data; D016337:RNA, Catalytic / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000378:metabolism*; D019065:Virus Assembly
D016337:RNA, Catalytic; D012367:RNA, Viral