Effects of fumonisin B1 in pregnant rats. Part 2.
The developmental toxicity of purified fumonisin B1 (FB1), a mycotoxin from the common corn fungus Fusarium moniliforme, was examined in Charles River rats. Pregnant rats were dosed orally on gestation days 3-16 at 0, 6.25, 12.5, 25 or 50 mg FB1/kg body weight/day. FB1 was not teratogenic at the doses tested. At 50 mg/kg, maternal toxicity (inappetence, emaciation, lethargy, death, resorption of entire litters) and foetal toxicity (increased number of late deaths, decreased foetal body weight, decreased crown rump length, increased incidence of hydrocephalus, increased incidence of skeletal anomalies) were seen. The foetal toxicity observed at 50 mg/kg may be related to maternal toxicity. Histopathological evaluation of tissues from dams of control and all treated groups revealed dose-related toxic changes in kidney and liver tissues. Acute toxic tubular nephrosis was seen in kidneys from all treated groups. Hepatocellular cytoplasmic alteration and individual cellular necrosis of the liver was seen in the two high-dose groups. Sphinganine (Sa) and sphingosine (So) were measured in day-17 adult and foetal tissues. Dose related increases in Sa/So ratios were seen in maternal liver, kidney, serum and brain, but there was no effect on foetal liver, kidney and brain. These data suggest that FB1 does not cross the placenta and further suggest that the observed foetal toxicity is a secondary response to maternal toxicity.
Collins
T F
TF
Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, MD 20708, USA.
Sprando
R L
RL
Black
T N
TN
Shackelford
M E
ME
Laborde
J B
JB
Hansen
D K
DK
Eppley
R M
RM
Trucksess
M W
MW
Howard
P C
PC
Bryant
M A
MA
Ruggles
D I
DI
Olejnik
N
N
Rorie
J I
JI
D000818:Animals; D002264:Carboxylic Acids / Q000633:toxicity*; D004435:Eating / Q000187:drug effects; D005314:Embryonic and Fetal Development / Q000187:drug effects; D005260:Female; D005333:Fetus / Q000473:pathology; D037341:Fumonisins; D007668:Kidney / Q000196:embryology / Q000473:pathology; D008099:Liver / Q000196:embryology / Q000473:pathology; D008297:Male; D009183:Mycotoxins / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000187:drug effects*; D051381:Rats; D011919:Rats, Inbred Strains; D012098:Reproduction / Q000187:drug effects; D013107:Sphingolipids / Q000378:metabolism; D013723:Teratogens / Q000633:toxicity*; D015430:Weight Gain / Q000187:drug effects
D002264:Carboxylic Acids; D037341:Fumonisins; D009183:Mycotoxins; D013107:Sphingolipids; D013723:Teratogens; C056933:fumonisin B1