Partial purification of hydrogenase from Anabaena sp. CH3 with heat treatment.
The methylviologen-dependent hydrogenase of Anabaena sp. CH3 is unstable at 4 degrees C and in air. All the purifying procedures were carried out at 25 degrees C and under argon atmosphere. The enzyme was partially purified by the following steps: heat treatment, DEAE-cellulose ion-exchange chromatography and gel filtration on TSK-Fractogel. Experimental results indicated that the heat-treatment procedure was beneficial for the separation of the enzyme from phycobiliproteins.
Wang
S H
SH
Department of Botany, National Chung Hsing University, Taichung, Taiwan, R.O.C.
Chen
P C
PC
D017033:Anabaena / Q000201:enzymology*; D006358:Hot Temperature; D006864:Hydrogenase / Q000302:isolation & purification*
D006864:Hydrogenase