A monoclonal antibody that recognizes a common carbohydrate epitope shared by various glycoproteins in human secretions.
A monoclonal antibody (mAb; a mouse IgM referred to as 1CF11) recognizing various human glycoproteins was obtained. While the immunoreaction of glycoproteins from human secretions including milk, saliva, and bronchus was demonstrated as a typical dose-responded S-shaped reaction curve on ELISA, no reaction was detected with milks and sera of animal origin as well as human serum. In the constituting polypeptides of the human milk secretory IgA molecule, only the secretory component was recognized by this mAb. Among various chemical treatments of the purified human milk lactoferrin (Lf), only either periodate or mild alkaline treatment abolished the immunoreactivity of the glycoprotein. A recombinant human Lf was not immunoreactive. Finally, the immunoreactive fragments were isolated from human milk Lf, which remained reactive with PAS reagent while lacking the previously reported N-glycans. These results strongly suggest that the mAb 1CF11 recognizes a new glycan O-glycosidically linked to glycoproteins in human secretions.
Kanamaru
Y
Y
Department of Food Science, Faculty of Agriculture, Gifu University, Japan. kanamaru@cc.gifu-u.ac.jp
Ikeda
S
S
Yang
S M
SM
Kaneko
T
T
Kuwata
T
T
Nagaoka
S
S
Shimizu
M
M
Sachdev
G P
GP
D000818:Animals; D000911:Antibodies, Monoclonal; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007075:Immunoglobulin M; D007781:Lactoferrin / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008892:Milk / Q000737:chemistry / Q000276:immunology; D008895:Milk, Human / Q000737:chemistry / Q000276:immunology; D008969:Molecular Sequence Data; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000737:chemistry / Q000276:immunology; D013045:Species Specificity; D013183:Sputum / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D007075:Immunoglobulin M; D007781:Lactoferrin