title: In vivo translation of the triple gene block of potato virus X requires two subgenomic mRNAs.
abstract: The 25-kilodalton (25K), 12K, and 8K movement proteins of potato virus X are derived from overlapping open reading frames (ORFs). Using an in vivo complementation assay, we have shown that the 25K protein is expressed from a functionally monocistronic mRNA, whereas the 12K and 8K proteins are from a bicistronic mRNA. Translation of the 8K ORF is by leaky ribosome scanning through the 12K ORF.
authors.affiliation: Sainsbury Laboratory, Norwich Research Park, Colney, United Kingdom NR4 7UH.
authors.forename: J
authors.initials: J
authors.lastname: Verchot
authors.forename: S M
authors.initials: SM
authors.lastname: Angell
authors.forename: D C
authors.initials: DC
authors.lastname: Baulcombe
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D016366:Open Reading Frames; D017863:Potexvirus / Q000235:genetics*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral