[Detection using molecular biology techniques of Mycoplasma hominis and Ureaplasma urealyticum in urogenital samples].
Mycoplasma hominis and Ureaplasma urealyticum are species closely related to urogenital diseases such as pyelonephritis, nongonococcal urethritis, urinary calculi, epididymitis, pelvic inflammation, infertility, abortions and post-delivery fever. They can also cause pneumonia and meningitis in newborn infants. In this paper we used nucleic acid hybridization and polymerase chain reaction to analyze 22 samples from patients with different urogenital symptoms in order to detect mycoplasmas and ureaplasmas. We obtained 10 positive samples and 12 were negative. From positive samples we identified two with Mycoplasma hominis, two with Ureaplasma and six with both species. The results obtained by these molecular techniques were compared with reference methods and we found coincident results in 18 samples, while in four the results were discordant. These discordant findings were not statistically significant.
Fernández
C
C
Instituto de Medicina Tropical Pedro Kouŕi, La Habana, Cuba. ciipk@inforned.sld.cu
Alvarez
K
K
Muy
L
L
Martínez
M
M
D001437:Bacteriuria / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004275:DNA, Ribosomal / Q000032:analysis*; D005069:Evaluation Studies as Topic; D005260:Female; D005798:Genes, Bacterial; D020459:Genes, rRNA; D006801:Humans; D009175:Mycoplasma Infections / Q000382:microbiology*; D019535:Mycoplasma hominis / Q000235:genetics / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D016869:Ureaplasma Infections / Q000382:microbiology*; D016990:Ureaplasma urealyticum / Q000235:genetics / Q000302:isolation & purification*; D014526:Urethritis / Q000382:microbiology*; D002575:Uterine Cervicitis / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S