Kinetics of bradykinin levels during and after leucocyte filtration of platelet concentrates.
OBJECTIVE
To measure bradykinin levels in platelet concentrates during and after leucocyte filtration.


METHODS
Platelet concentrates were leukocyte depleted using three different leucocyte-depletion filters selected to represent filters with negative, positive or neutral charge, respectively. Bradykinin levels were analysed before, during and up to 90 min post-filtration.


RESULTS
Significant levels of bradykinin were generated when negatively charged leucocyte depletion filters were used. However, we found a high variation between platelet concentrates prepared from different donors as well as within the same concentrate when this was divided into three aliquots. Generated bradykinin was rapidly degraded in the collection bag and no bradykinin was detectable 60 min post-filtration.


CONCLUSIONS
Bradykinin generation is more pronounced when negatively charged leucocyte removal filters are used. Due to a rapid degradation of bradykinin in the storage bag, pre-storage filtration should minimise the risk of bradykinin related adverse reactions during transfusion with some filters.
Hild
M
M
Department of Clinical Immunology, Karolinska Hospital, Stockholm, Sweden.
Söderström
T
T
Egberg
N
N
Lundahl
J
J
D000293:Adolescent; D000328:Adult; D000368:Aged; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D001672:Biocompatible Materials; D001792:Blood Platelets; D001920:Bradykinin / Q000097:blood*; D018821:CD18 Antigens / Q000032:analysis; D015926:Complement C3a / Q000032:analysis; D015773:Enalaprilat / Q000494:pharmacology; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007700:Kinetics; D007937:Leukapheresis; D007958:Leukocyte Count; D008212:Lymphocyte Depletion / Q000379:methods*; D016177:Macrophage-1 Antigen / Q000032:analysis; D008875:Middle Aged; D055672:Static Electricity; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D001672:Biocompatible Materials; D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D015926:Complement C3a; D015773:Enalaprilat; D001920:Bradykinin