Dual rearrangement of immunoglobulin and T-cell receptor genes in a case of Philadelphia chromosome-positive acute leukemia.
A 48-year-old patient was admitted to our hospital for leukocytosis. The blast cells were positive for peroxidase and he was tentatively diagnosed as acute myeloid leukemia according to the French-American-British criteria. By flow cytometry, the bone marrow cells were positive for CD10, CD13, CD33 and HLA-DR, but two-color analysis revealed that most of the CD13- and CD33-positive cells did not express CD10. The marrow cells had Philadelphia chromosome with no additional abnormalities. Major bcr-abl fusion gene was observed by the reverse transcriptase-polymerase chain reaction method. Southern blot analysis disclosed rearrangement of both immunoglobulin heavy chain and T-cell receptor beta chain genes. He received combined chemotherapy for myeloid lineage and lymphoid lineage, but the response was quite poor. He died 64 days after admission due to pulmonary bleeding. Although the association of Ph1 with multilineage differentiation is unclear, our case has significant implication for further investigation of the relationship between Ph1-positive cells and lineage selection.
Wakimoto
N
N
The Third Department of Internal Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.
Mukai
Y
Y
Kuwada
N
N
Yamashita
T
T
Matsumura
T
T
Nakamura
Y
Y
Kimura
F
F
Sato
K
K
Nagata
N
N
Motoyoshi
K
K
D000208:Acute Disease; D001853:Bone Marrow / Q000276:immunology; D018826:CD13 Antigens / Q000032:analysis; D016044:Fusion Proteins, bcr-abl / Q000032:analysis; D015329:Gene Rearrangement, T-Lymphocyte; D005803:Genes, Immunoglobulin; D019674:Genes, T-Cell Receptor beta; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015260:Neprilysin / Q000032:analysis; D016133:Polymerase Chain Reaction
D006684:HLA-DR Antigens; D016044:Fusion Proteins, bcr-abl; D018826:CD13 Antigens; D015260:Neprilysin