title: Distinct roles of oncostatin M and leukemia inhibitory factor in the development of primordial germ cells and sertoli cells in mice.
abstract: Leukemia inhibitory factor (LIF) stimulates the growth of primordial germ cells (PGCs) in mouse embryo. However, as neither mice lacking LIF nor mice lacking the LIF receptor show defects in PGC growth, an alternate cytokine for PGC growth has been postulated. We investigated the role of mouse oncostatin M (mOSM), which is structurally and functionally related to LIF, in germ cell development. While LIF enhanced the survival of migratory as well as postmigratory PGCs, mOSM acted only on the postmigratory PGCs. Consistent with its biological activity, mOSM was found to be expressed in developing gonads. In the male, Sertoli cells in neonatal testis express mOSM; however, its expression is downregulated in adult testes. Moreover, mOSM enhanced the proliferation of Sertoli cells derived from neonatal testes in vitro more than human OSM or LIF. In contrast, postnatal ovaries do not express mOSM. These results indicate that mOSM is a stage- and sex-specific autocrine growth factor for Sertoli cells.
authors.affiliation: Institute of Molecular and Cellular Biosciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-0032, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Hara
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: M P
authors.initials: MP
authors.lastname: de Miguel
authors.forename: Y
authors.initials: Y
authors.lastname: Mukouyama
authors.forename: H j
authors.initials: Hj
authors.lastname: Kim
authors.forename: H
authors.initials: H
authors.lastname: Kogo
authors.forename: P J
authors.initials: PJ
authors.lastname: Donovan
authors.forename: A
authors.initials: A
authors.lastname: Miyajima
mesh_terms: D000818:Animals; D002455:Cell Division / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D016207:Cytokines / Q000494:pharmacology; D005314:Embryonic and Fetal Development / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D005854:Germ Cells / Q000187:drug effects*; D006066:Gonads / Q000166:cytology / Q000254:growth & development; D006131:Growth Inhibitors / Q000494:pharmacology*; D006651:Histocytochemistry; D006801:Humans; D017403:In Situ Hybridization; D007265:Inhibins; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains / Q000196:embryology; D053683:Oncostatin M; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000187:drug effects*
chemical_list: D016207:Cytokines; D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; C508351:OSM protein, human; C508352:Osm protein, mouse; D010455:Peptides; D012333:RNA, Messenger; C094377:inhibin-alpha subunit; D053683:Oncostatin M; D007265:Inhibins