Detection of the leghemoglobin gene on two chromosomes of Phaseolus vulgaris by in situ PCR linked-fluorescent in situ hybridization (FISH).
The leghemoglobin (Lb) gene on the metaphase chromosomes of Phaseolus vulgaris was amplified by in situ PCR. The amplified Lb gene could be detected on two chromosomes by fluorescent in situ hybridization (FISH) using the short Lb gene probe.
Uchiumi
T
T
Department of Chemistry and BioScience, Faculty of Science, Kagoshima University, Japan.
Kuwashiro
R
R
Miyamoto
J
J
Abe
M
M
Higashi
S
S
D001483:Base Sequence; D002874:Chromosome Mapping; D018744:DNA, Plant; D007887:Fabaceae / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007874:Leghemoglobin / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction / Q000379:methods*
D018744:DNA, Plant; D007874:Leghemoglobin