CpG islands detected by self-primed in situ labeling (SPRINS).
We have studied the distribution and methylation of CpG islands on human chromosomes, using the novel technique of self-primed in situ labeling (SPRINS). The SPRINS technique is a hybrid of the two techniques primed in situ labeling (PRINS) and nick translation in situ. SPRINS detects chromosomal DNA breaks, as in nick translation in situ, and not annealed primers, as is the case in PRINS. We analyzed in situ-generated DNA breaks induced by the restriction enzymes HpaII and MspI. These restriction enzymes enable the detection of chromosomal CpG islands. Both HpaII- and MspI-SPRINS produce a banding pattern resembling R-banding, indicating a higher level of CpG islands in R-positive bands than in R-negative bands. Our SPRINS banding observations also indicate differences in sequence copy number in the satellites of homologous acrocentric chromosomes. Furthermore, a comparison of homologous HpaII-SPRINS-banded X chromosomes of females from lymphocyte cultures grown without methotrexate or bromodeoxyuridine revealed methylation difference between them. The same comparison of homologous X chromosomes from the cell line GM01202D, which has four X chromosomes, one active and three inactive, revealed the active X chromosome to be hypermethylated.
Andersen
C L
CL
The Danish Cancer Society, Department of Cytogenetics, Tage Hansens Gade 2, DK-8000 Aarhus C, Denmark.
Koch
J
J
Kjeldsen
E
E
D002478:Cells, Cultured / Q000187:drug effects; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D018899:CpG Islands; D019175:DNA Methylation; D017931:DNA Primers; D004276:DNA, Satellite / Q000032:analysis; D019003:Deoxyribonuclease HpaII; D004303:Dosage Compensation, Genetic; D006570:Heterochromatin / Q000235:genetics / Q000648:ultrastructure; D006801:Humans; D017403:In Situ Hybridization; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D008727:Methotrexate / Q000494:pharmacology; D014960:X Chromosome / Q000235:genetics / Q000648:ultrastructure
D017931:DNA Primers; D004276:DNA, Satellite; D006570:Heterochromatin; D019003:Deoxyribonuclease HpaII; D008727:Methotrexate