Catabolic fate of dietary trilinoleoylglycerol hydroperoxides in rat gastrointestines.
To elucidate whether dietary lipid peroxides are absorbed in the body, the catabolic fate of trilinoleoylglycerol hydroperoxides (TL-OOH), in the gastrointestines of rats was examined. Oxidized trilinoleoylglycerol with a peroxide value of 1000 meq/kg, 0.5 or 20 mg, was dosed intragastrically to rat together with 59.5 or 40 mg unoxidized trilinoleoylglycerol, respectively. The fate of TL-OOH in gastric and intestinal lumina was determined by high-performance liquid chromatography periodically until 240 min after treatment. At low dose, TL-OOH was soon broken down to linoleic acid hydroperoxides (LA-OOH) and hydroxyls, probably through gastric lipases, whereas at high dose, TL-OOH was retained in the stomach. In both cases, TL-OOH did not reach the intestines, though the unoxidized lipids moved to the intestines. When LA-OOH was given intragastrically, the lipids decomposed in the stomach, and linoleic acid hydroxyls, hexanal, 9-oxononanoic acid, and two novel compounds were detected 30 min after treatment. The novel compounds were identified to be epoxyketones, 11-oxo-12,13-epoxy-9- and 11-oxo-9,10-epoxy-12-octadecenoic acids. Thus, dietary TL-OOH was broken down in the stomach releasing, LA-OOH which decomposed further, and did not reach the intestines.
Kanazawa
K
K
Laboratory of Food and Nutritional Chemistry, Faculty of Agriculture, Kobe University, Japan. kazuki@kobe-u.ac.jp
Ashida
H
H
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000378:metabolism; D005751:Gastric Lavage; D005753:Gastric Mucosa / Q000378:metabolism*; D007422:Intestines / Q000737:chemistry; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D019787:Linoleic Acid / Q000378:metabolism; D008054:Lipid Peroxides / Q000008:administration & dosage / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D008627:Mercuric Chloride / Q000008:administration & dosage; D009829:Oleic Acids / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000737:chemistry; D013997:Time Factors
D004042:Dietary Fats, Unsaturated; D004852:Epoxy Compounds; D007659:Ketones; D008054:Lipid Peroxides; D009829:Oleic Acids; D008627:Mercuric Chloride; D019787:Linoleic Acid