Rat oligodendrocyte O-2A precursor cells and the CG-4 oligodendrocyte precursor cell line express cadherins, beta-catenin and the neural cell adhesion molecule, NCAM.
Oligodendrocyte precursor cell migration throughout the developing central nervous system (CNS) and cessation of migration are poorly understood but are likely to involve cell adhesion molecules. The expression and distribution of neural cell adhesion molecule (NCAM), cadherins and beta-catenin were investigated in the CG-4 cell line and primary rat oligodendrocyte progenitor cells (O-2A) by immunofluorescence and Western blotting. NCAM was expressed by both cell types and was found all over the surface of both CG-4 cells and O-2A progenitor glia. The presence of a cadherin was detected in both CG-4 cells and O-2A progenitor glia, and this molecule was distributed all over the cell body and cell processes at different stages of differentiation. Beta-catenin showed a very similar distribution to that of the cadherin. We conclude that CG-4 cells are a valid model system to study cell adhesion molecule expression and function in oligodendrocyte progenitor cells.
Hughson
E
E
Department of Biology, York University, UK. ejh7@york.ac.uk
Dowler
S
S
Geall
K
K
Johnson
G
G
Rumsby
M
M
D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western; D001921:Brain / Q000166:cytology / Q000378:metabolism; D015820:Cadherins / Q000096:biosynthesis*; D002460:Cell Line; D019070:Cell Lineage; D002465:Cell Movement; D003598:Cytoskeletal Proteins / Q000096:biosynthesis*; D005455:Fluorescent Antibody Technique; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis*; D009836:Oligodendroglia / Q000378:metabolism*; D051381:Rats; D013234:Stem Cells / Q000378:metabolism*; D015534:Trans-Activators; D051176:beta Catenin
D015820:Cadherins; C495268:Ctnnb1 protein, rat; D003598:Cytoskeletal Proteins; D019006:Neural Cell Adhesion Molecules; D015534:Trans-Activators; D051176:beta Catenin