title: Cytogenetic adaptive response in cultured human lymphocytes: dependence on the time of exposure to adapting and challenging doses of gamma-rays.
abstract: Human lymphocytes from 16 healthy donors were exposed in vitro to an adapting dose of gamma-rays (0.05 Gy) at G0, or G1, or G1/S stage of the cell cycle and subsequently to a challenging dose of gamma-rays at G1, or G1/S, or S (1 Gy), or G2 (0.5 Gy) stage. Frequencies and distributions of the induced chromosome aberrations were analyzed in first-division metaphases. The data averaged over the donors revealed the protective action of the adapting exposure under the irradiation schemes with the challenging dose delivered at S or G2 stage. The majority of aberrations induced at these stages belonged to the chromatid type, and their yield was significantly higher in G2-exposed cells than in S-exposed cells. However, the relative reduction of the challenging dose effect (about 34%) in the adapted cells did not depend on the magnitude of this effect, and its value remained the same (within the experimental error) if aberrations were subdivided into chromosome and chromatid types or grouped as total deletions and total fragments. The adaptive response was not revealed under the schemes with the challenging dose delivered at G1 or G1/S stage. Analysis of the individual results showed that, in one and the same donor, the adaptive response could be observed under one irradiation scheme and not observed under other schemes, the most effective schemes being those with the challenging dose delivered at G2 stage. Four donors, however, did not show the adaptive response even under such schemes. Data on aberration distributions suggested that different repair processes, rather than a unique one, may underlie the adaptive response.
authors.affiliation: Medical Radiological Research Center, Russian Academy of Medical Sciences, Ul. Koroleva 4, Obninsk 249090, Russian Federation.
authors.forename: N I
authors.initials: NI
authors.lastname: Ryabchenko
authors.forename: M M
authors.initials: MM
authors.lastname: Antoshchina
authors.forename: E V
authors.initials: EV
authors.lastname: Fesenko
authors.forename: T I
authors.initials: TI
authors.lastname: Ivanova
authors.forename: T V
authors.initials: TV
authors.lastname: Kondrashova
authors.forename: V A
authors.initials: VA
authors.lastname: Nasonova
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005260:Female; D005720:Gamma Rays; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000528:radiation effects*; D008297:Male; D012016:Reference Values; D013997:Time Factors