title: An ex vivo study of T lymphocytes recovered from the lungs of I/St mice infected with and susceptible to Mycobacterium tuberculosis.
abstract: I/St mice, previously characterized as susceptible to Mycobacterium tuberculosis H37Rv, were given 10(3) or 10(5) CFU intravenously. At two time points postinoculation, the cell suspensions that resulted from enzymatic digestion of lungs were enumerated and further characterized phenotypically and functionally. Regarding the T-cell populations recovered at 2 and 5 weeks postinfection, two main results were obtained: (i) the population of CD44(-) CD45RB+ cells disappeared within 2 weeks postinfection, while the number of CD44(+) CD45RB-/low cells slowly increased between weeks 2 and 5; (ii) when cocultured with irradiated syngeneic splenocytes, these lung T cells proliferated in the presence of H37Rv sonicate. Using H37Rv sonicate and irradiated syngeneic splenocytes to reactivate lung T cells, we selected five CD3(+) CD4(+) CD8(-) T-cell clones. In addition to the H37Rv sonicate, the five clones react to both a short-term culture filtrate and an affinity-purified 15- to 18-kDa mycobacterial molecule as assessed by the proliferative assay. However, there was a clear difference between T-cell clones with respect to cytokine (gamma interferon [IFN-gamma] and interleukin-4 [IL-4] and IL-10) profiles: besides one Th1-like (IFN-gamma+ IL-4(-)) clone and one Th0-like (IFN-gamma+ IL-4(+) IL-10(+)) clone, three clones produced predominantly IL-10, with only marginal or no IL-4 and IFN-gamma responses. Inhibition of mycobacterial growth by macrophages in the presence of T cells was studied in a coculture in vitro system. It was found that the capacity to enhance antimycobacterial activity of macrophages fully correlated with INF-gamma production by individual T-cell clones following genetically restricted recognition of infected macrophages. The possible functional significance of cytokine diversity among T-cell clones is discussed.
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow 107564, Russia. asapt@aha.ru
authors.forename: I
authors.initials: I
authors.lastname: Lyadova
authors.forename: V
authors.initials: V
authors.lastname: Yeremeev
authors.forename: K
authors.initials: K
authors.lastname: Majorov
authors.forename: B
authors.initials: B
authors.lastname: Nikonenko
authors.forename: S
authors.initials: S
authors.lastname: Khaidukov
authors.forename: T
authors.initials: T
authors.lastname: Kondratieva
authors.forename: N
authors.initials: N
authors.lastname: Kobets
authors.forename: A
authors.initials: A
authors.lastname: Apt
mesh_terms: D000818:Animals; D015703:Antigens, CD; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells; D018920:Coculture Techniques; D004198:Disease Susceptibility; D005260:Female; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D008168:Lung / Q000166:cytology / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000472:pathogenicity*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma