Molecular analysis of chitin synthase 1 (CHS1) gene sequences of Trichophyton mentagrophytes complex and T. rubrum.
Nucleotide sequences of chitin synthase 1 (CHS1) gene of dermatophytes, Arthroderma benhamiae, A. simii, A. vanbreuseghemii, Trichophyton mentagrophytes var. interdigitale (T. interdigitale), and T. rubrum were analyzed for their phylogenetic relationship. About 620-bp genomic DNA fragments of the CHS1 gene were amplified from these dermatophytes by polymerase chain reaction (PCR) and sequenced. The CHS1 nucleotide sequences of these five dermatophytes showed more than 90% similarity between the species. The phylogenetic analysis of their sequences revealed that A. benhamiae, A. simii, A. vanbreuseghemii, and T. rubrum were genetically distinct from one another, but T. interdigitale was genetically very close to A. vanbreuseghemii. On the other hand, a specific restriction endonuclease site of HinfI was present in the CHS1 gene fragment of T. rubrum but not in those of A. benhamiae, A. simii, A. vanbreuseghemii and T. interdigitale. The molecular analysis of CHS1 genes will provide useful information for the identification of these Trichophyton species and the understanding of their evolution.
Kano
R
R
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Nakamura
Y
Y
Watari
T
T
Watanabe
S
S
Takahashi
H
H
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D001483:Base Sequence; D002687:Chitin Synthase / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D004285:Dogs; D005800:Genes, Fungal; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D013045:Species Specificity; D014249:Trichophyton / Q000145:classification / Q000201:enzymology* / Q000235:genetics*
D017931:DNA Primers; D004271:DNA, Fungal; D002687:Chitin Synthase