Effects of 2-deoxy-D-glucose administration on immune parameters in mice.
Physical exercise and diet alterations have been shown to affect immune parameters. Similar effects are also induced by the administration of the non-metabolizable glucose analog, 2-deoxy-D-glucose (2-DG). The current study was designed to characterize the effects of glucoprivation induced by 2-DG administration on leukocyte subset distribution and function. BDF1 mice (n = 8 per group) were injected intraperitoneally one or three times with 0, 500, 750, 1000 or 1500 mg/kg of 2-DG. Two hours after the last injection of 2-DG, immunological parameters were analyzed. A dose-dependent increase in plasma glucose concentrations of mice injected once with up to 1500 mg/kg of 2-DG was observed (p < 0.001). After either one or three injections of up to 1500 mg/kg of 2-DG, corticosterone levels, leukocyte counts in the spleen, and CD3+ cells in the thymus increased. In vitro proliferation of partially purified lymphocytes from the spleen in the presence of both concanavalin-A and lipopolysaccharide decreased in a dose dependent manner (p < 0.05). In addition, after three injections, the proportion of both thymocytes and splenocytes bearing alphabeta-TCR increased as the concentration of 2-DG increased (p < 0.01). These results demonstrate that 2-DG administration induced dose-dependent changes in both thymus and spleen cell distribution and function.
Dréau
D
D
Dept. General Surgery Research, Carolinas Medical Center, Charlotte, NC 28203, USA. ddreau@carolinas.org
Morton
D S
DS
Foster
M
M
Fowler
N
N
Sonnenfeld
G
G
D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D001786:Blood Glucose / Q000378:metabolism; D003345:Corticosterone / Q000097:blood; D003847:Deoxyglucose / Q000494:pharmacology*; D005260:Female; D019344:Lactic Acid / Q000097:blood; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D016131:Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D013950:Thymus Gland / Q000166:cytology / Q000276:immunology
D000963:Antimetabolites; D001786:Blood Glucose; D019344:Lactic Acid; D003847:Deoxyglucose; D003345:Corticosterone