23Na NMR study of the interaction between DNA and the platinum (II) compounds: cis-DDP, trans-DDP and TDP.
The interaction of calf thymus DNA with cis-DDP, trans-DDP and TDP was studied by 23Na NMR in aqueous solutions at pH=7.0, with Pt(II) compounds/DNA(P) (P=Phosphate) molar ratios r increasing from 0 till to 1P. 23Na NMR results are interpreted on one hand, with the help of a " two states model " with R(F) and R(B) relaxation rates, and, on the other hand, using the " entropy of fluctuations " concept developed by Lenk. We have established that, for the studied platinum compounds, the preference to interact with DNA phosphate sites -interpreted as a perturbation of the counterions environment- is in a decreasing order: TDP >> cis-DDP > trans-DDP. These results are discussed with regard to the interaction of DNA with the hard bication Mg++ and the soft bication Cu++.
Mallet
G
G
Laboratoire de Biophysique - GRECFO - Université de Nice-Sophia Antipolis Parc Valrose, Nice, France.
Ansiss
S
S
Vasilescu
D
D
D000818:Animals; D002417:Cattle; D002945:Cisplatin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012979:Sodium Radioisotopes; D012996:Solutions; D013696:Temperature
D012979:Sodium Radioisotopes; D012996:Solutions; C034697:transplatin; D004247:DNA; D002945:Cisplatin