title: Identification of tomato Lhc promoter regions necessary for circadian expression.
abstract: Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions are present at different positions relative to the transcription start site of Lhc b1*1, b1*2, Lhc a3 and Lhc a4. A short sequence of 47 nucleotides is necessary for conferring circadian Lhc mRNA oscillations. Sequence alignment of the specified promoter regions revealed a novel motif 'CAANNNNATC'. This motif is conserved in 5'-upstream regions of clock controlled Lhc genes and overlaps with a sequence relevant in phytochrome mediated gene expression.
authors.affiliation: Universität Rostock, Biochemie, Fachbereich Biologie, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Piechulla
authors.forename: N
authors.initials: N
authors.lastname: Merforth
authors.forename: B
authors.initials: B
authors.lastname: Rudolph
mesh_terms: D001483:Base Sequence; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology*; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D018551:Solanum lycopersicum / Q000235:genetics* / Q000502:physiology*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D045322:Photosynthetic Reaction Center Complex Proteins; D012333:RNA, Messenger; D018749:RNA, Plant