Mobilization of lead by calcium versenate and dimercaptosuccinate in the rat.
1. Calcium disodium ethylenediaminetetraacetate (CaNa2 EDTA) and meso-2,3-dimercaptosuccinic acid (DMSA) individually and in permutation-combination in various doses (0.1, 0.2 and 0.4 mmol/kg bodyweight) were investigated for their efficacy to mobilize lead from vital tissues into urine and faeces and to restore the lead-sensitive biochemical parameters in lead pre-exposed rats with a view to develop the most acceptable treatment regimen for lead poisoning with a minimal loss of endogenous essential elements. 2. The combined therapy was more effective than a single chelator treatment. 3. The combination of 0.2 mmol/kg CaNa2EDTA + 0.4 mmol/kg DMSA caused a lower depletion of zinc, calcium and iron but possessed almost equal capability to that of 0.4 mmol/kg CaNa2EDTA + 0.4 mmol/kg DMSA to produce urinary as well as faecal excretion of lead, to reduce the tissue burden of lead, including that of the brain, and to reverse lead-induced biochemical alterations. 4. The combination of 0.2 mmol/kg CaNa2EDTA + 0.4 mmol/kg DMSA has shown a definite improvement over previously reported combinations in terms of removal of lead from tissues, particularly the brain, restoration of urinary delta-aminolevulinic acid levels and a decrease in the loss of body zinc and is, therefore, recommended for the treatment of lead intoxication.
Tandon
S K
SK
Industrial Toxicology Research Centre, Lucknow, India. tandonintox@itrc.sirnetd.irnet.in
Singh
S
S
Prasad
S
S
Mathur
N
N
D000818:Animals; D001822:Body Burden; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology*; D007501:Iron / Q000378:metabolism; D007854:Lead / Q000378:metabolism*; D008297:Male; D051381:Rats; D004113:Succimer / Q000494:pharmacology*; D015032:Zinc / Q000378:metabolism
D002614:Chelating Agents; D007854:Lead; D004492:Edetic Acid; D004113:Succimer; D007501:Iron; D015032:Zinc; D002118:Calcium