title: Successful polymerase chain reaction-based diagnosis of fungal meningitis in a patient with chronic granulomatous disease.
abstract: Meningitis is not a common complication of chronic granulomatous disease (CGD). Here, we present details of a 3-year-old boy with X-linked CGD, who suffered from fungal meningitis. While 19 samplings using conventional cerebrospinal fluid (CSF) cultures failed to detect any organisms, fungal DNA was identified in the CSF by a new polymerase chain reaction (PCR)-based method. The patient recovered without any sequelae after treatment with a combination of antifungal agents, interferon-gamma and granulocyte infusions. This case report demonstrates that fungal meningitis must be included in the differential diagnosis of infections in CGD patients and that the PCR-based detection of fungal DNA is a powerful tool for diagnosis.
authors.affiliation: Department of Pediatrics, Nagoya University School of Medicine, Japan. itsuge@med.nagoya-u.ac.jp
authors.forename: I
authors.initials: I
authors.lastname: Tsuge
authors.forename: K
authors.initials: K
authors.lastname: Makimura
authors.forename: J
authors.initials: J
authors.lastname: Natsume
authors.forename: T
authors.initials: T
authors.lastname: Kubota
authors.forename: S
authors.initials: S
authors.lastname: Hasegawa
authors.forename: T
authors.initials: T
authors.lastname: Kawabe
authors.forename: S
authors.initials: S
authors.lastname: Nakashima
authors.forename: K
authors.initials: K
authors.lastname: Aso
authors.forename: T
authors.initials: T
authors.lastname: Negoro
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D002675:Child, Preschool; D004271:DNA, Fungal / Q000032:analysis; D006105:Granulomatous Disease, Chronic / Q000150:complications*; D006801:Humans; D008297:Male; D016921:Meningitis, Fungal / Q000175:diagnosis*; D016133:Polymerase Chain Reaction
chemical_list: D004271:DNA, Fungal