A new subculture and nematocidal assay using a species of diplogastridae.
A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4 h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known compounds, including the antiparasitic avermectin, antimalarial quinine, and the gamma-amino-n-butyric acidA (GABAA) activated Cl- channel antagonist picrotoxinin. Thus new assay could be used as a primary screening method for new nematocidal compounds.
Yanagida
J
J
School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Matsuhashi
R
R
Watanabe
I
I
Satou
T
T
Koike
K
K
Nikaido
T
T
Akao
S
S
D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D009348:Nematoda / Q000187:drug effects* / Q000254:growth & development*
D000969:Antinematodal Agents; D003470:Culture Media