Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for oxyuroid species (Nematoda).
Random amplified DNA markers (RAPD; Williams et al., 1990) were used to obtained specific RAPD fragments characterising different species of oxyuroids. We tested six species of worms parasitizing vertebrates or invertebrates: Passalurus ambiguus Rudolphi, 1819, parasite of Leporids; Syphacia obvelata (Rudolphi, 1802) Seurat, 1916, a parasite of rodents; Blatticola blattae (Graeffe, 1860) Chitwood, 1932 parasite of the cockroach Blattella germanica; Hammerschmidtiella diesingi (Hammerschmidt, 1838) Chitwood, 1932 and Thelastoma bulhoesi (Magalhaes, 1990) Travassos, 1929, parasites of the cockroach Periplaneta americana, and an undescribed parasite species of a passalid insect from New Caledonia. Among 15 oligonucleotides tested, nine produced several specific bands allowing the interspecific discrimination.
Jobet
E
E
Laboratoire de Parasitologie-Mycologie, Hôpital Ambroise-Paré, Boulogne.
Bougnoux
M E
ME
Morand
S
S
Rivault
C
C
Cloarec
A
A
Hugot
J P
JP
D000818:Animals; D003058:Cockroaches / Q000469:parasitology; D017931:DNA Primers / Q000737:chemistry; D015342:DNA Probes / Q000737:chemistry* / Q000235:genetics; D018509:DNA, Helminth / Q000032:analysis* / Q000235:genetics; D005260:Female; D007313:Insecta / Q000469:parasitology; D007793:Lagomorpha / Q000469:parasitology; D008297:Male; D009115:Muridae / Q000469:parasitology; D010124:Oxyuroidea / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D013045:Species Specificity
D017931:DNA Primers; D015342:DNA Probes; D018509:DNA, Helminth