Expression of activation surface markers, interleukin-2 synthesis and apoptosis rate in fresh or cultured lymphocytes from HIV-infected children.
In 2 groups of HIV-infected children aged from 7 to 10 years (ARC and AIDS, respectively) the following immune markers: % expression of CD69, CD25, HLA-DR activation surface determinants, IL-2 synthesis, rate of apoptosis were tested in non stimulated, PHA-stimulated or PHA and IL-12-stimulated T cell cultures. In all HIV-originated cells a decrease of CD69 expression and an increase of CD25 and HLA-DR expression were found. A strong correlation could be noticed between the clinical stage of the AIDS infection and the in vitro IL-2 production and the percentages of apoptotic cultured cells. IL-12 supplementation of PHA-stimulated cell samples restored the IL-2 synthesis and reduced the apoptosis rates only in the ARC-group, but not in the AIDS-group. The significance of the present data in the clinical and therapeutic monitoring of HIV infection among low-aged people are discussed.
Voiculescu
C
C
Faculty of Medicine, Department of Microbiology and Immunology, Craiova, Romania.
Avramescu
C
C
Radu
E
E
Bălăşoiu
M
M
Turculeanu
A
A
D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D017209:Apoptosis; D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D002648:Child; D015658:HIV Infections / Q000276:immunology*; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007376:Interleukin-2 / Q000096:biosynthesis*; D037181:Lectins, C-Type; D008214:Lymphocytes / Q000276:immunology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D006684:HLA-DR Antigens; D007376:Interleukin-2; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D000099192:CD69 Antigens