Bcl-2 is located predominantly in the inner membrane and crista of mitochondria in rat liver.
Bcl-2 is now recognized as a potent inhibitor of apoptotic cell death. It has been reported that Bcl-2 is located in the mitochondria, endoplasmic reticulum, and nuclear membrane in some cell lines, and it is not expressed in normal human and rat liver. An earlier study showed that Bcl-2 is an inner mitochondrial membrane protein. On the contrary, the following investigations using immunoelectron microscopy demonstrated that Bcl-2 resides predominantly in the mitochondrial outer membrane. In this study, using a cryo-sectioning immunogold labeling technique and immunoblotting, we carefully determined the subcellular localization of Bcl-2. Here we report that Bcl-2 is expressed in normal rat liver, and it is located predominantly in the inner membrane and crista rather than in the outer membrane of mitochondria.
Motoyama
S
S
Second Department of Surgery, Akita University School of Medicine, Japan.
Kitamura
M
M
Saito
S
S
Minamiya
Y
Y
Suzuki
H
H
Saito
R
R
Terada
K
K
Ogawa
J
J
Inaba
H
H
D000818:Animals; D002458:Cell Fractionation; D018922:HL-60 Cells; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D016253:Microscopy, Immunoelectron; D008930:Mitochondria, Liver / Q000378:metabolism* / Q000648:ultrastructure*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013367:Submitochondrial Particles / Q000378:metabolism / Q000648:ultrastructure
D019253:Proto-Oncogene Proteins c-bcl-2