Beta1 integrin deficiency impairs migration and differentiation of mouse embryonic stem cell derived neurons.
Cell-matrix interaction plays an important role during neuronal development, which is demonstrated by comparing wild type (D3)- and beta1 integrin-deficient (G201) embryonic stem cell derived neurons. In D3 preparations complex networks of functionally coupled neurons with bi- and multipolar morphologies develop. In contrast, neuronal differentiation is retarded in G201 derived neurons, recognised by limited migration and restricted morphological differentiation. Furthermore, beta1 integrin deficiency causes a delay in expression of major neurotransmitters like GABA and glutamate as well as of synaptophysin. These findings indicate a prominent role of beta1 integrin for both morphological and chemical differentiation.
Andressen
C
C
Institut I für Anatomie der Universität zu Köln, Germany. christian.andressen@uni-koeln.de
Arnhold
S
S
Puschmann
M
M
Bloch
W
W
Hescheler
J
J
Fässler
R
R
Addicks
K
K
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000502:physiology*; D018698:Glutamic Acid / Q000096:biosynthesis; D007150:Immunohistochemistry; D019012:Integrin beta1 / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D016708:Synaptophysin / Q000096:biosynthesis; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis
D019012:Integrin beta1; D016708:Synaptophysin; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid