[Production of recombinant human keratinocyte growth factor in Escherichia coli cells].
Here we describe the synthesis of the gene encoding human keratinocyte growth factor (hKGF) and the construction of vectors for cytoplasmic production of hKGF in Escherichia coli cells. The level of recombinant protein expression was 1-1.5% of the total cell protein. hKGF was purified to homogeneity by three-step ion-exchange and affinity chromatography. The protein is biologically active: it stimulates dose-dependent protein synthesis in cultured human epidermal keratinocytes.
Ptitsyn
L R
LR
State Research Institute Genetika, Moscow, Russia.
Al'tman
I B
IB
Gurov
M V
MV
Vasil'ev
A V
AV
Voroteliak
E A
EA
Terskikh
V V
VV
D000595:Amino Acid Sequence; D001483:Base Sequence; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D051526:Fibroblast Growth Factor 10; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors; D015870:Gene Expression; D005822:Genetic Vectors; D006133:Growth Substances / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
C496453:FGF7 protein, human; D051526:Fibroblast Growth Factor 10; D006133:Growth Substances; D011994:Recombinant Proteins; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors