Protection by rebamipide against acetic acid-induced colitis in rats: relationship with its antioxidative activity.
The aim of this study was to examine the efficacy of rebamipide on acetic acid-induced colitis in rats. Colitis was induced in male Wistar/ST strain rats by intracolonic injection of 2 ml of 5% acetic acid. After 24 hr, lesion score, myeloperoxidase (MPO) activity, thiobarbituric acid-reactive substances (TBARS), and antioxidants were measured. Rebamipide was administered orally to the rats twice, once immediately and once 8 hr after the induction of the colitis at doses of 7.5, 15, and 30 mg/kg. To evaluate the pathogenesis of neutrophils in this colitis model, neutrophil-depleted rats were also investigated. Increases of TBARS and MPO activity and decreases of total glutathione, superoxide dismutase (SOD) activity, and alpha-tocopherol were observed in this model. Rebamipide at 30 mg/kg decreased the lesion score significantly, which was associated with the reduction of colonic TBARS. The decreases of total glutathione, SOD activity, and alpha-tocopherol were also inhibited significantly by rebamipide. The preventive effect of rebamipide in this colitis model may be attributed to its inhibition of reactive oxygen species production.
Sakurai
K
K
Microbiological Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
Osaka
T
T
Yamasaki
K
K
D019342:Acetic Acid; D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D003092:Colitis / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007413:Intestinal Mucosa / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D015363:Quinolones / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D015363:Quinolones; D017382:Reactive Oxygen Species; C052785:rebamipide; D000409:Alanine; D019342:Acetic Acid