title: In vitro antigenotoxic activity of novel ginseng saponin metabolites formed by intestinal bacteria.
abstract: Ginseng saponin metabolites produced by human intestinal bacteria were evaluated for antigenotoxic properties by testing their effects on benzo[a]pyrene (B[a]P)-induced mutagenicity and clastogenicity. They include 20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol (IH-901), 20-O-(alpha-D-arabinopyranosyl(1-->6)-beta-D-glucopyranosyl]- 20(S)-protopanaxadiol (IH-902) and 20-O-[alpha-D-arabinofuranosyl(1-->6)-beta-D-glucopyranosyl]-20(S)- protopanaxadiol (IH-903). IH-901, IH-902 and IH-903 inhibited the mutagenicity of B[a]P in a dose-dependent manner. In the chromosome aberration assay, IH-901 and IH-903 reduced the frequency of chromosome aberration induced by B[a]P. These results suggest that the ginseng saponin metabolites tested in the present study have potential as chemopreventive agents.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, Chonbuk, Korea. bhlee@wonnms.wonkwang.ac.kr
authors.forename: B H
authors.initials: BH
authors.lastname: Lee
authors.forename: S J
authors.initials: SJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Hur
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Sung
authors.forename: J D
authors.initials: JD
authors.lastname: Huh
authors.forename: C K
authors.initials: CK
authors.lastname: Moon
authors.forename: J H
authors.initials: JH
authors.lastname: Hui
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000737:chemistry* / Q000494:pharmacology*; D001564:Benzo(a)pyrene / Q000037:antagonists & inhibitors / Q000633:toxicity*; D002460:Cell Line; D002869:Chromosome Aberrations; D006224:Cricetinae; D003412:Cricetulus; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008168:Lung; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009152:Mutagenicity Tests; D005894:Panax; D010946:Plants, Medicinal; D051381:Rats; D017207:Rats, Sprague-Dawley; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*; D012503:Saponins / Q000378:metabolism* / Q000494:pharmacology*; D012854:Sister Chromatid Exchange
chemical_list: D016587:Antimutagenic Agents; D012503:Saponins; D001564:Benzo(a)pyrene