title: Energy-dependent redox state of heme a + a3 and copper of cytochrome oxidase in perfused rat brain in situ.
abstract: Using the blood-free perfused rat brain, we examined the redox behavior of cytochrome oxidase of two chromophores, heme a + a3 and copper. When perfusate inflow was stopped to induce global ischemia, the reduction of heme a + a3 was triphasic, with a rapid phase, a slow phase, and a second rapid phase. In contrast, the reduction of copper was monophasic after the rapid phase of heme a + a3. The triphasic reduction of heme a + a3 was diminished by energy-depleting treatments, such as addition of an uncoupler. The time course of the reduction of copper was not affected by the energy depletion. During global ischemia the decrease in creatine phosphate nearly paralleled the reduction of heme a + a3, whereas ATP remained at the control level until approximately 60% of heme a + a3 was reduced in the rapid phase. In the slow phase, ATP started to decrease with the reduction of copper. The redox behavior of copper was similar to the slow phase of the reduction of heme a + a3 because of the higher oxygen affinity of copper than of heme a + a3. Therefore, the rapid phase of the reduction of heme a + a3 can be used as an alarm before a decrease in ATP, whereas the reduction of copper indicates a decrease in ATP under severe hypoxia. Thus the copper signal in noninvasive near-infrared spectroscopy is a useful parameter for the clinical setting.
authors.affiliation: Biophysics Group, Research Institute for Electronic Science, Hokkaido University, Sapporo 060, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Matsunaga
authors.forename: Y
authors.initials: Y
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Kuroda
authors.forename: M
authors.initials: M
authors.lastname: Tamura
authors.forename: J
authors.initials: J
authors.lastname: Nishihira
authors.forename: N
authors.initials: N
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000502:physiology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D003300:Copper / Q000378:metabolism*; D004569:Electroencephalography; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004734:Energy Metabolism; D006418:Heme / Q000031:analogs & derivatives* / Q000378:metabolism; D007003:Hypoglycemia; D002534:Hypoxia, Brain / Q000378:metabolism / Q000503:physiopathology; D002546:Ischemic Attack, Transient / Q000201:enzymology / Q000503:physiopathology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010725:Phosphocreatine; C027728:heme a; D006418:Heme; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D003300:Copper; D003576:Electron Transport Complex IV