title: Bb1-3, a transgenic hybrid cell line with erythroid and megakaryocytic differentiation potential that expresses high levels of human gamma-globin and human beta-globin.
abstract: We have characterized a murine hybrid cell line, Bb1-3, generated by the fusion of mouse primary erythroblasts with MEL cells. It proliferated in serum-free medium and displayed a low level of spontaneous erythroid and megakaryocyte differentiation. Terminal erythroid differentiation could be induced with HMBA and DMSO and was enhanced by serum. Treatment with phorbol esters resulted in a high proportion of megakaryocytes and the expression of megakaryocytic specific lineage markers. Bb1-3 cells contain a human beta-globin transgene that was expressed at levels of 20-50% of the endogenous mouse globin genes. Initially, expression was largely limited to the beta-globin gene but after adaptation to serum free growth, equal expression of both the human gamma- and human beta-globin genes was observed. This cell line provides further evidence that the differentiation potential of mouse erythroleukaemia cells is not restricted to the erythroid lineage and should be useful to study the mechanisms underlying both developmental globin gene regulation and the terminal differentiation of bipotential erythroid/megakaryocytic progenitor cells.
authors.affiliation: MRC Molecular Haematology Unit, Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital.
authors.forename: A M
authors.initials: AM
authors.lastname: Thomson
authors.forename: N A
authors.initials: NA
authors.lastname: Roberts
authors.forename: W G
authors.initials: WG
authors.lastname: Wood
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D016895:Culture Media, Serum-Free; D004247:DNA / Q000032:analysis; D003850:Deoxyribonuclease I / Q000235:genetics; D004912:Erythrocytes / Q000166:cytology*; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D005914:Globins / Q000235:genetics / Q000378:metabolism*; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D006801:Humans; D006825:Hybridomas; D008533:Megakaryocytes / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D014407:Tumor Cells, Cultured
chemical_list: D016895:Culture Media, Serum-Free; D016298:Hematopoietic Cell Growth Factors; D005914:Globins; D004247:DNA; D003850:Deoxyribonuclease I