title: Effects of various flow types on maternal hemodynamics during fetal bypass: is there nitric oxide release during pulsatile perfusion?
abstract: OBJECTIVE
  This study investigates the role of various flow conditions on maternal hemodynamics during fetal cardiopulmonary bypass.
  
  
  METHODS
  Normothermic fetal bypass was conducted under pulsatile, or steady flow, for a 60-minute period. Fetal lamb preparations were randomly assigned to 1 of the 3 groups: steady flow (n=7), pulsatile flow (n=7), or pulsatile blocked flow bypass (n=7), where fetuses were perfused with Nomega-nitro-L-arginine after the first 30 minutes of pulsatile flow to assess the potential role of endothelial autacoids.
  
  
  RESULTS
  Maternal oximetry and pressures remained unchanged throughout the procedure. Under fetal pulsatile flow, maternal cardiac output increased after 20 minutes of bypass and remained significantly higher than under steady flow at minute 30 (8.8+/-0.7 L x min(-1) vs 5.9+/-0.5 L x min(-1), P=.02). Maternal cardiac output in the pulsatile group also remained higher than in both steady and pulsatile blocked flow groups, reaching respectively 8.7+/-0.9 L x min(-1) vs 5.8+/-0.4 L x min(-1) (P=.02) and 5.9+/-0.3 L min(-1) (P=.01) at minute 60. Maternal systemic vascular resistances were significantly lower under pulsatile than under steady flow after 30 minutes and until the end of bypass (respectively, 9.1+/-0.6 IU vs 12.7+/-1.1 IU, P=.02 and 8.9+/-0.5 IU vs 12.9+/-1.2 IU, P=.01). Infusion of Nomega-nitro-L-arginine was followed by an increase in systemic vascular resistances from 9.3+/-0.7 IU, similar to that of the pulsatile group, to 13.5+/-1 IU at 60 minutes, similar to that of the steady flow group.
  
  
  CONCLUSIONS
  Maternal hemodynamic changes observed under fetal pulsatile flow are counteracted after infusion of Nomega-nitro-L-arginine, suggesting nitric oxide release from the fetoplacental unit under pulsatile fetal flow conditions.
authors.affiliation: Department of Anesthesia and Intensive Care Medicine in Cardiovascular Surgery, Louis Pradel Cardiologic Hospital, Lyon, France.
authors.forename: C
authors.initials: C
authors.lastname: Vedrinne
authors.forename: F
authors.initials: F
authors.lastname: Tronc
authors.forename: S
authors.initials: S
authors.lastname: Martinot
authors.forename: J
authors.initials: J
authors.lastname: Robin
authors.forename: C
authors.initials: C
authors.lastname: Garhib
authors.forename: J
authors.initials: J
authors.lastname: Ninet
authors.forename: J J
authors.initials: JJ
authors.lastname: Lehot
authors.forename: M
authors.initials: M
authors.lastname: Franck
authors.forename: G
authors.initials: G
authors.lastname: Champsaur
mesh_terms: D000818:Animals; D002302:Cardiac Output / Q000502:physiology; D002315:Cardiopulmonary Bypass; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005318:Fetal Heart / Q000502:physiology; D005333:Fetus / Q000601:surgery*; D006439:Hemodynamics / Q000502:physiology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D019335:Nitroarginine / Q000494:pharmacology; D021041:Placental Circulation / Q000502:physiology*; D011247:Pregnancy; D011673:Pulsatile Flow / Q000502:physiology; D012756:Sheep; D013997:Time Factors; D014655:Vascular Resistance / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D019335:Nitroarginine; D009569:Nitric Oxide