[Serum factors in cancer patients affecting the antitumor effect of 5-fluorouracil].
A human colon cancer cell line, HCT-15, was found to proliferate in human sera. Sera of cancer patients undergoing continuous intravenous administration of 5-fluorouracil (5-FU) were tested for the antitumor effect by counting HCT-15 cells stained with Giemsa solution. 5-FU concentrations in those sera were not correlated with the antitumor effects. After HCT-15 cells were incubated in sera of cancer patients or healthy volunteers for 24 hours followed by removal of those sera, those cells were incubated in a culture medium containing 500 or 1000 ng/ml of 5-FU for 48 hours. It was shown that preincubation of HCT-15 cells with sera of cancer patients but not sera of healthy volunteers decreased the sensitivity of HCT-15 cells to 5-FU. These results suggest that there may be some factors in the serum of a cancer patient, which affect the antitumor effect of 5-FU.
Miwa
M
M
First Dept. of Surgery, Aichi Medical University.
Kojima
T
T
Naruse
T
T
D000964:Antimetabolites, Antineoplastic / Q000097:blood / Q000494:pharmacology*; D003110:Colonic Neoplasms / Q000097:blood* / Q000473:pathology*; D005472:Fluorouracil / Q000097:blood / Q000494:pharmacology*; D006801:Humans; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil