title: Insulin down-regulates angiotensinogen gene expression and angiotensinogen secretion in cultured adipose cells.
abstract: Adipose tissue is an important source of angiotensinogen (AT) after liver. Since an association exists between body mass index, hypertension, and insulin-resistance, the role of insulin on the regulation of AT gene expression and AT secretion was examined in cultured Ob1771 and 3T3-F442A adipose cells. Within a physiological range of concentrations (1-17 nM), insulin exerted a negative effect on the abundance of AT mRNA and the secretion of AT. Alterations of insulin-resistance by treatment of adipose cells with TNF-alpha or the thiazolidinedione BRL49653 led respectively to a decrease or an increase in the potency of insulin to down-regulate AT gene expression, whereas maximal inhibition by insulin increased from 30% in TNFalpha-treated cells to 60% in BRL49653-treated cells. These results suggest that a potential link between insulin resistance and high blood pressure may exist by means of increased AT secretion from adipose tissue, especially in obese subjects.
authors.affiliation: Faculté des Sciences, Université de Nice-Sophia Antipolis, Parc Valrose, Nice cedex 2, 06108, France.
authors.forename: J
authors.initials: J
authors.lastname: Aubert
authors.forename: I
authors.initials: I
authors.lastname: Safonova
authors.forename: R
authors.initials: R
authors.lastname: Negrel
authors.forename: G
authors.initials: G
authors.lastname: Ailhaud
mesh_terms: D016475:3T3 Cells; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000808:Angiotensinogen / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics; D000077154:Rosiglitazone; D013844:Thiazoles / Q000494:pharmacology; D045162:Thiazolidinediones; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D007004:Hypoglycemic Agents; D007328:Insulin; D012333:RNA, Messenger; D013844:Thiazoles; D045162:Thiazolidinediones; D014409:Tumor Necrosis Factor-alpha; D000077154:Rosiglitazone; D000808:Angiotensinogen