Proliferative effects of oxidized low-density lipoprotein on vascular smooth muscle cells: role of dietary habits.
The effects were studied of native, partially-oxidized and totally-oxidized human low-density lipoprotein (LDL) on the proliferation of cultured rat aortic smooth muscle cells (VSMC), measured as an altered DNA synthesis. The LDL was obtained from three different human long-term diet groups (a control diet rich in saturated fats, a vegetarian diet, and a fish diet). The oxidized LDLs were prepared by oxidizing the LDL with copper sulfate. The DNA synthesis was measured by [3H]-thymidine incorporation into the DNA. The partially-oxidized LDL was the most potent promoter of DNA synthesis compared to the native or totally-oxidized LDL of the same diet group. The partially-oxidized LDL had a true mitogenic effect in the absence of exogenous growth factors. The native and totally-oxidized LDL induced a significant increase in DNA synthesis, if they were obtained from the fish diet group. This study suggests an enhanced proliferative effect of partially-oxidized LDL on VSMC growth.
Lähteenmäki
T A
TA
Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki, Finland. tuula.lahteenmaki@helsinki.fi
Korpela
R
R
Tikkanen
M J
MJ
Karjala
K
K
Laakso
J
J
Solatunturi
E
E
Vapaatalo
H
H
D000328:Adult; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D004032:Diet; D014676:Diet, Vegetarian; D005247:Feeding Behavior; D005260:Female; D005399:Fishes; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000494:pharmacology*; D008297:Male; D008460:Meat; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects*; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013936:Thymidine / Q000378:metabolism
D008077:Lipoproteins, LDL; D004247:DNA; D013936:Thymidine