Serum IgA antibodies from patients with coeliac disease react strongly with human brain blood-vessel structures.
BACKGROUND
Our objective was to determine the possible presence of IgA antibodies directed against human central nervous system (CNS) structures in sera from coeliac disease (CD) patients.


METHODS
Serum samples were collected from 4 patients with active CD on a gluten-containing diet, 11 biopsy-proven CD patients on a gluten-free diet (GFD), and 52 non-coeliac gastrointestinal controls. In all patients IgA antigliadin antibody (AGA) titres were determined with enzyme-linked immunoassay (ELISA), and IgA antiendomysium antibodies (EMA) with indirect immunofluorescence on human umbilical cord. Cryostat sections of human brain occipital cortex were incubated with the patients' sera and subsequently labelled with anti-human IgA fluorescein conjugate.


RESULTS
All sera from patients with active CD on a gluten-containing diet yielded positive results in both the IgG-AGA and EMA test and in indirect immunofluorescence on brain tissue, disclosing a strong fluorescence over blood-vessels structures. All sera from CD patients on a GFD and from non-coeliac gastrointestinal controls gave a negative result on both the EMA test and the immunofluorescence reaction on human brain.


CONCLUSIONS
Sera from patients with active CD contain IgA antibodies that react with human brain vessel structures, giving intense fluorescence. These antibodies are not present in sera from coeliac patients on a GFD or non-coeliac controls. This finding might be involved in the abnormal nervous system manifestations frequently described in association with coeliac disease.
Pratesi
R
R
Dept. of Pediatrics, Brasilia University, Brazil.
Gandolfi
L
L
Friedman
H
H
Farage
L
L
de Castro
C A
CA
Catassi
C
C
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D001921:Brain / Q000098:blood supply* / Q000276:immunology*; D016022:Case-Control Studies; D002446:Celiac Disease / Q000276:immunology*; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D019084:Fluorescent Antibody Technique, Indirect; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007223:Infant; D008297:Male; D008875:Middle Aged
D001323:Autoantibodies; D007070:Immunoglobulin A; D005903:Gliadin