Heterologous gene expression in an Escherichia coli population under starvation stress conditions.
A novel system to study the evolution of transcription signals in heterologous systems under selective starvation conditions is described. It is based on the plasmid-mediated transfer of his biosynthetic genes from Azospirillum brasilense into a heterologous Escherichia coli mutant population lacking histidine biosynthetic ability. We show that under highly selective stressful conditions, genetic changes in the donor plasmid lead to mutated sequences that are efficiently recognized as promoters by the E. coli RNA polymerase.
Fani
R
R
Gallo
R
R
Fancelli
S
S
Mori
E
E
Tamburini
E
E
Lazcano
A
A
D000222:Adaptation, Physiological / Q000235:genetics; D016949:Azospirillum / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D019020:Directed Molecular Evolution; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development*; D019143:Evolution, Molecular; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006639:Histidine / Q000096:biosynthesis / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
D006639:Histidine; D012321:DNA-Directed RNA Polymerases