title: Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus.
abstract: The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate.
authors.affiliation: Department of Microbiology and Biotechnology, Kossuth Lajos University, Debrecen, Hungary.
authors.forename: E
authors.initials: E
authors.lastname: Sándor
authors.forename: T
authors.initials: T
authors.lastname: Pusztahelyi
authors.forename: L
authors.initials: L
authors.lastname: Karaffa
authors.forename: Z
authors.initials: Z
authors.lastname: Karányi
authors.forename: I
authors.initials: I
authors.lastname: Pócsi
authors.forename: S
authors.initials: S
authors.lastname: Biró
authors.forename: A
authors.initials: A
authors.lastname: Szentirmai
authors.forename: I
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000164:Acremonium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis*; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D014312:Trisaccharides / Q000494:pharmacology*
chemical_list: D002511:Cephalosporins; D004791:Enzyme Inhibitors; D014312:Trisaccharides; C054851:allosamidin; C025163:cephalosporin C; D002688:Chitinases; D000117:Acetylglucosamine