title: Self-amplification of satellite DNA in vitro.
abstract: We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.
authors.affiliation: Institute of Infectious Diseases and Immunology, Utrecht University, The Netherlands.
authors.forename: J B
authors.initials: JB
authors.lastname: Buntjer
authors.forename: J A
authors.initials: JA
authors.lastname: Lenstra
mesh_terms: D000818:Animals; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics*; D016678:Genome; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
chemical_list: D004276:DNA, Satellite