Retrospective dose reconstruction of human radiation exposure by FISH/chromosome painting.
Measurements of stable chromosomal aberrations by fluorescence in situ hybridisation (FISH)-chromosome painting has the potential to be used for dose reconstruction, years after exposure to ionising radiation. The method is, however, not yet fully standardised and validated. In particular, with respect to the limited lifespan of circulating T-lymphocytes, a level of uncertainty exists on the long-term persistence of stable aberrations. The main principles of the technique will be demonstrated. Based on results from the literature, the reliability and limitations are discussed.
Bauchinger
M
M
GSF-National Research Center for Environment and Health, Institute of Radiobiology, Ingolstädter Landstr. 1, 85758 Oberschleissheim, Germany. bauchinger@gsf.de
D002138:Calibration; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D015342:DNA Probes / Q000235:genetics; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011829:Radiation Dosage; D011874:Radiometry; D014178:Translocation, Genetic / Q000235:genetics
D015342:DNA Probes; D005456:Fluorescent Dyes