title: Following the folding of RNA with time-resolved synchrotron X-ray footprinting.
abstract: The rapid mixing synchrotron X-ray footprinting technique described in this article allows nucleic acid folding and ligand binding reactions to be followed on a millisecond time resolution with single nucleotide resolution. In principle, the change in .OH protection of every nucleotide in a nucleic acid hundreds of nucleotides long can be monitored separately. In addition, a wide range of solution conditions are compatible with the radiolytic generation of .OH. These characteristics of synchrotron X-ray footprinting create opportunities for conducting thermodynamic and kinetic studies of nucleic acids that are both comprehensive and detailed. Kinetic footprinting studies of a number of systems have been initiated by the Center for Synchrotron Biosciences using this technique.
authors.affiliation: Department of Physiology and Biophysics, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
authors.forename: B
authors.initials: B
authors.lastname: Sclavi
authors.forename: S
authors.initials: S
authors.lastname: Woodson
authors.forename: M
authors.initials: M
authors.lastname: Sullivan
authors.forename: M
authors.initials: M
authors.lastname: Chance
authors.forename: M
authors.initials: M
authors.lastname: Brenowitz
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods*; D017665:Hydroxyl Radical; D007700:Kinetics; D008024:Ligands; D008274:Magnesium / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D017356:Synchrotrons; D014965:X-Rays
chemical_list: D008024:Ligands; D017665:Hydroxyl Radical; D012313:RNA; D008274:Magnesium