Effect of alpha-tocopherol on mitochondrial electron transport in experimental myocardial infarction in rats.
The effect of alpha-tocopherol pretreatment (6 mg/100 g body wt/day, orally for a period of 90 days) on mitochondrial electron transport in myocardial infarction induced by isoproterenol (20 mg/100 g body wt, subcutaneously for two days) was studied in rats. A significant decrease was observed in the activities of isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and cytochrome oxidase in heart mitochondria of isoproterenol administered rats. The cytochrome content and the oxidation of succinate in state 3 and state 4 decreased significantly in the cardiac mitochondria treatment. In alpha-tocopherol pretreated rats, the activities of TCA cycle enzymes, concentration of cytochromes and the oxidation of succinate in state 3 and state 4 were retained at near normal values, following isoproterenol administration.
Ithayarasi
A P
AP
Department of Biochemistry, University of Madras, Chennai, India.
Shyamala Devi
C
C
D000818:Animals; D002952:Citric Acid Cycle / Q000187:drug effects; D003580:Cytochromes / Q000378:metabolism; D004579:Electron Transport / Q000187:drug effects; D007545:Isoproterenol; D015227:Lipid Peroxidation / Q000187:drug effects; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism*; D009203:Myocardial Infarction / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D014810:Vitamin E / Q000494:pharmacology*
D003580:Cytochromes; D008054:Lipid Peroxides; D014810:Vitamin E; D007545:Isoproterenol