Prediction of selectivity for the separation of double-stranded DNA fragments in electrophoresis.
The electrophoretic resolution (selectivity) of double-stranded DNA (dsDNA) restriction fragments is studied for different organisms (phi X174, lambda phage, and pGEM-3 plasmids) by various separation and detection methods. The selectivity can be predicted by plotting the difference in chain length over their average chain length (base pair difference/average base pair magnitude of delta bp/Ave.-bp) against each DNA fragment pair. Experimental conditions and the nature of the DNA can influence the predicted results.
Otim
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Department of Chemistry, Wichita State University, Kansas 67260-0051, USA.
Singhal
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D004279:DNA, Viral / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000379:methods; D010957:Plasmids / Q000302:isolation & purification*
D004279:DNA, Viral