[Etiology and diagnostic of viral bronchopneumonias].
Community viral bronchopneumonias are frequent, mainly in children, and can be associated to all respiratory viruses: influenza- and parainfluenzavirus, respiratory syncytial virus, adenovirus, rhinovirus. The diagnostic method which proves viral infection of the respiratory tissues is selected as the direct detection by an immunofluorescence assay of viral infected cells in respiratory samples. In them, viral isolation or nucleic acid detection by PCR provide an amplification of the viruses. By using PCR-hybridation techniques viral detection is overall increased of 1.5 times for respiratory syncytial virus, 1.9 for parainfluenzavirus 3, 4 for rhinovirus and 10 times for adenovirus. This increased sensitivity raises questions about the meaning of the detection of viral sequences in nasal aspirates, with or without clinical signs. Cytomegalovirus (CMV) is a major agent of pneumonia in immunocompromised patients. All virological markers of CMV infection have to be sought (antigenemia, viremia...), but specific inclusions in pulmonary cells are the single diagnosis criteria. As pulmonary biopsies are rarely available and CMV inclusions rarely found in BAL, it has been reported useful to look for high viral loads or late m-RMA transcripts in these samples. Adenovirus pneumonia are unfrequent in these patients and mostly associated to rare or atypical strains. Such PCR-hybridization systems deserves also to be used in these cases.
Freymuth
F
F
Laboratoires de virologie humaine et moléculaire, Hôpital universitaire, Caen.
Vabret
A
A
Galateau
F
F
Brouard
J
J
Eugene
G
G
Petitjean
J
J
Gennetay
E
E
D015415:Biomarkers; D001706:Biopsy; D001992:Bronchoalveolar Lavage Fluid / Q000821:virology; D001996:Bronchopneumonia / Q000175:diagnosis* / Q000821:virology*; D002648:Child; D017714:Community-Acquired Infections / Q000175:diagnosis* / Q000821:virology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D011024:Pneumonia, Viral / Q000175:diagnosis* / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014776:Virus Cultivation
D015415:Biomarkers