Biochemical and histological analysis of the flexor tenosynovium in patients with carpal tunnel syndrome.
Carpal tunnel release is the most common hand operation performed in this country. In the absence of specific systemic diseases, the etiology and persistence of pain and dysfunction even after surgical decompression is poorly understood. The focus of this investigation was to investigate the biological factors present within the patients serum that may lead to increased sensitivity to pain. Tissue was collected from patients during surgery. The tissue was homogenized and the homogenate analyzed for the presence of IL-1, IL-6, prostaglandin E series (PGE2). The levels were compared with volunteers that had no evidence of carpal tunnel syndrome or pain. The results showed similar levels of IL-1 (range 42-26 ng/ml) in tissue homogenates, and a significant increase in levels of IL-6 and malionaldehyde bis-(diethyl acetal) in CTS patients in comparison to control tissues. This increase may be associated with oxidative changes occurring as a result of ischemia and reperfusion. Tissue homogenates were also evaluated for PGE2. The CTS tissues showed a five fold elevation in PGE2 compared to control tissues. Levels of PGE2 in CTS tissues were statistically different using a two-tailed student T-test. Increased levels of PGE2 can enhance vascular permeability at the site of injury, and can play an important role in activating adenylate cyclase which increases intracellular cyclic adenosine monophosphate (cAMP). This increase in cAMP levels can inhibit functional responses to other inflammatory stimuli. Increases in PGE2 can also cause sensitization of the nerve endings so that a normal stimulus that would not necessarily cause pain will now be experienced as painful. The results of this study demonstrate that arachidonic acid metabolites PGE2 may be responsible for both the pathological changes and clinical symptomatology in carpal tunnel syndrome.
Tucci
M A
MA
Department of Orthopaedic Surgery, University of Mississippi Medical Center, Jackson 39216, USA.
Barbieri
R A
RA
Freeland
A E
AE
D002349:Carpal Tunnel Syndrome / Q000378:metabolism* / Q000473:pathology; D015232:Dinoprostone / Q000032:analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007375:Interleukin-1 / Q000032:analysis; D015850:Interleukin-6 / Q000032:analysis; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008875:Middle Aged; D013583:Synovial Membrane / Q000737:chemistry* / Q000473:pathology; D013710:Tendons / Q000737:chemistry* / Q000473:pathology
D007375:Interleukin-1; D015850:Interleukin-6; D008315:Malondialdehyde; D015232:Dinoprostone