Dendritic cells from skin and blood of macaques both promote SIV replication with T cells from different anatomical sites.
The SIV-macaque system offers the opportunity to study the pathogenesis and immune aspects of a primate retroviral infection in which immunodeficiency also develops, much like HIV infection in humans. Since it is known that human dendritic cells (DCs) are involved in HIV replication, mature cytokine-generated DCs obtained from precursors in the blood and skin-derived DCs were isolated from healthy rhesus macaques and compared with respect to their ability to support SIV infection. Here, it is shown for both skin- and blood-derived DCs that i) virus production depends on both DCs and T cells, ii) this occurs similarly with T cells from blood, skin, spleen, or lymph nodes, and iii) DCs can transmit virus equally to syngeneic and allogeneic T cells. No differences between DCs from skin or blood were observed. Therefore, the easily accessible blood-derived DCs of macaques provide an appropriate population to study the role of DCs in immunodeficiency virus infection.
Ignatius
R
R
Laboratory of Cellular Physiology and Immunology, The Rockefeller University, New York, New York, USA.
Isdell
F
F
O'Doherty
U
U
Pope
M
M
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D018920:Coculture Techniques; D003713:Dendritic Cells / Q000166:cytology / Q000187:drug effects / Q000821:virology*; D005260:Female; D015683:Gene Products, gag / Q000096:biosynthesis; D015726:Giant Cells; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology; D008198:Lymph Nodes / Q000276:immunology; D008253:Macaca mulatta; D011994:Recombinant Proteins / Q000494:pharmacology; D015302:Simian Immunodeficiency Virus / Q000502:physiology*; D012867:Skin / Q000166:cytology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000821:virology*; D014779:Virus Replication
C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D011994:Recombinant Proteins; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor