title: Expression of the RNA component of telomerase during human development and differentiation.
abstract: We used a radioactive in situ method to study expression of the RNA component of human telomerase (hTR) during normal human development and differentiation using archival tissues. In embryonic tissues, the highest and most uniform expression was present in undifferentiated neuroepithelium. Expression was stronger in immature epithelium than in accompanying immature mesenchyme. Differentiation of most tissues was accompanied by decreased or absent expression. Except for testis and adrenal, the adult pattern of expression was present by the 10th postnatal week. In adult tissues, high expression was present in the testis (primary spermatocytes and Sertoli cells), moderate expression was present in lymphoid follicles (germinal centers), and weak expression was present in epithelia (regenerative cells) but was absent in the nervous system and mesenchymal derived tissues. Expression in adult tissues was predominantly limited to dividing cells, although certain differentiated postmitotic cells expressed the hTR. Our studies demonstrate the complex interrelationship of hTR expression with human development, differentiation, and cell division.
authors.affiliation: Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: K
authors.initials: K
authors.lastname: Yashima
authors.forename: A
authors.initials: A
authors.lastname: Maitra
authors.forename: B B
authors.initials: BB
authors.lastname: Rogers
authors.forename: C F
authors.initials: CF
authors.lastname: Timmons
authors.forename: A
authors.initials: A
authors.lastname: Rathi
authors.forename: H
authors.initials: H
authors.lastname: Pinar
authors.forename: W E
authors.initials: WE
authors.lastname: Wright
authors.forename: J W
authors.initials: JW
authors.lastname: Shay
authors.forename: A F
authors.initials: AF
authors.lastname: Gazdar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002490:Central Nervous System / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D002648:Child; D002675:Child, Preschool; D004622:Embryo, Mammalian / Q000201:enzymology; D005260:Female; D005333:Fetus / Q000201:enzymology; D015870:Gene Expression; D005865:Gestational Age; D006066:Gonads / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D006413:Hematopoietic System / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D008221:Lymphoid Tissue / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D008297:Male; D008648:Mesoderm / Q000166:cytology / Q000201:enzymology; D008875:Middle Aged; D017933:Peripheral Nervous System / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D012137:Respiratory System / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D012867:Skin / Q000196:embryology / Q000201:enzymology / Q000254:growth & development; D019098:Telomerase / Q000235:genetics*; D014018:Tissue Distribution; D014566:Urogenital System / Q000196:embryology / Q000201:enzymology / Q000254:growth & development
chemical_list: D012313:RNA; D019098:Telomerase