Neutrophil chemiluminescence during phagocytosis is inhibited by abnormally elevated levels of acetoacetate: implications for diabetic susceptibility to infections.
Human neutrophils by a chemiluminescence assay exhibit diminished phagocytic activity in the presence of abnormally high levels of the serum metabolite acetoacetate. These findings, along with our previous evidence demonstrating myeloperoxidase inhibition by acetoacetate, implicate metabolic ketosis in the inhibition of neutrophil microbicidal activity and thus in increased susceptibility to infections.
Saeed
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FA
Department of Biology, St. Mary's College of California, Moraga, California 94575, USA. fsaeed@stmarys-ca.edu
Castle
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GE
D000090:Acetoacetates / Q000097:blood / Q000494:pharmacology*; D003920:Diabetes Mellitus / Q000276:immunology*; D004198:Disease Susceptibility / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008163:Luminescent Measurements; D018375:Neutrophil Activation / Q000187:drug effects; D009504:Neutrophils / Q000502:physiology*; D010587:Phagocytosis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000090:Acetoacetates; C016635:acetoacetic acid; D013755:Tetradecanoylphorbol Acetate