title: Carbonic anhydrase I reduction in colonic mucosa of patients with active ulcerative colitis.
abstract: Ulcerative colitis (UC) is associated with low intracolonic pH and unbalanced transmucosal ionic exchanges. Along the gastrointestinal tract carbonic anhydrase isoenzyme I (CA-I) is specifically expressed in colon epithelium and is involved in mucosal control of ion, fluid, and acid-base balance. Since altered CA-I expression may play some role in UC, CA-I was measured at the mRNA and protein level and carbonic anhydrase (CA) enzyme activity was determined in colon biopsies of 14 UC patients (6 remission, 4 mild, 4 moderate UC) and of 12 healthy subjects. Patients with mild or moderate UC showed a significant reduction of CA-I mRNA and protein and of total CA activity in the inflamed mucosa compared to controls. Patients with UC in remission showed a pattern of CA-I expression and CA activity similar to controls. This is the first report showing a reduction in the expression of CA-I in active UC.
authors.affiliation: Cattedra di Gastroenterologia, Università de L'Aquila, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Fonti
authors.forename: G
authors.initials: G
authors.lastname: Latella
authors.forename: R
authors.initials: R
authors.lastname: Caprilli
authors.forename: G
authors.initials: G
authors.lastname: Frieri
authors.forename: A
authors.initials: A
authors.lastname: Marcheggiano
authors.forename: Y
authors.initials: Y
authors.lastname: Sambuy
mesh_terms: D000208:Acute Disease; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000201:enzymology*; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D010084:Oxidation-Reduction; D012313:RNA / Q000032:analysis
chemical_list: D012313:RNA; D002256:Carbonic Anhydrases