title: Development of long PCR techniques to analyze deletion mutations of the human hprt gene.
abstract: DNA primers and reaction conditions for long polymerase chain reaction amplification (PCR) of portions of the human hprt gene are presented. Use of these primers with DNA from previously defined hprt deletion mutants demonstrated production of the expected smaller size products as compared to DNA from wild type cells. These primers will be of value in the rapid analysis and subsequent sequencing of hprt deletion mutations in human cells.
authors.affiliation: Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555, USA.
authors.forename: B
authors.initials: B
authors.lastname: Van Houten
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: J A
authors.initials: JA
authors.lastname: Nicklas
authors.forename: I R
authors.initials: IR
authors.lastname: Rainville
authors.forename: J P
authors.initials: JP
authors.lastname: O'Neill
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion
chemical_list: D017931:DNA Primers; D007041:Hypoxanthine Phosphoribosyltransferase