title: Corneal innervation and sensitivity to noxious stimuli in trkA knockout mice.
abstract: Most primary sensory neurones depend on neurotrophins for survival. Mutant mice in which TrkA, the high-affinity receptor for nerve growth factor (NGF), has been inactivated lack nociceptive neurones in sensory ganglia and do not respond to noxious stimuli. The cornea of the eye is innervated by trigeminal neurones that are activated by noxious mechanical, thermal and chemical stimuli. In the human cornea, these stimuli evoke only sensations of pain. We have analysed the innervation pattern and the response to noxious stimulation of the cornea of trkA (-/-) mutant mice. Corneal nerves were stained with the gold chloride impregnation method. Corneal sensitivity to noxious stimuli was assessed by counting blinking movements evoked by von Frey hairs, topical application of saline at different temperatures and application of acetic acid and capsaicin at different concentrations. In the cornea of trkA (-/-) mutant animals, we observed a drastic reduction in the number of nerve trunks and branches in the corneal stroma. Furthermore, quantitative analysis of the number of thin nerve terminals revealed a marked decrease in the corneal epithelium of trkA (-/-) mice when compared to those present in wild type and trkA (+/-) animals. The blinking response of trkA (-/-) mice to mechanical, thermal and chemical noxious stimuli was also significantly reduced. These results indicate that the population of corneal sensory neurones is markedly depleted in trkA (-/-) mutant mice. However, a small portion of corneal sensory neurones survive in these mice suggesting that they may be NGF independent. On the basis of our results, we propose that these surviving cells are polymodal nociceptive neurones, sensitive to mechanical stimulation, noxious heat and acid.
authors.affiliation: Instituto de Neurociencias, Universidad Miguel Hernandez, San Juan de Alicante, Spain.
authors.forename: F
authors.initials: F
authors.lastname: de Castro
authors.forename: I
authors.initials: I
authors.lastname: Silos-Santiago
authors.forename: M
authors.initials: M
authors.lastname: López de Armentia
authors.forename: M
authors.initials: M
authors.lastname: Barbacid
authors.forename: C
authors.initials: C
authors.lastname: Belmonte
mesh_terms: D000143:Acids / Q000494:pharmacology; D000818:Animals; D001767:Blinking / Q000502:physiology; D002211:Capsaicin / Q000494:pharmacology; D003315:Cornea / Q000294:innervation*; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009475:Neurons, Afferent / Q000187:drug effects* / Q000502:physiology; D009619:Nociceptors / Q000187:drug effects / Q000502:physiology; D009676:Noxae; D010146:Pain / Q000503:physiopathology; D010525:Peripheral Nerves / Q000187:drug effects* / Q000502:physiology; D010812:Physical Stimulation; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020917:Receptor, trkA; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D013268:Stimulation, Chemical
chemical_list: D000143:Acids; D009676:Noxae; D011518:Proto-Oncogene Proteins; D017475:Receptors, Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; D020917:Receptor, trkA; D002211:Capsaicin