title: Clinical significance of combined immunohistochemical detection of CD44v and sialyl LeX expression for colorectal cancer patients undergoing curative resection.
abstract: To evaluate their prognostic value, the expressions of CD44v and sialyl LeX (SLX) in colorectal cancers were studied immunohistochemically. Tissue specimens were reacted with monoclonal antibodies (mAb) CD44-1V and CSLEX-1. Of the 145 colorectal cancer patients undergoing curative resection, 59 (40.7%) were positive for mAb CD44-1V, and 40 (27.6%) were positive for mAb CSLEX-1. There was a significant correlation between the combined expression of SLX and CD44v8-10 and lymph node metastasis. The patients with tumors negative for CD44v8-10 and SLX had the most favorable prognoses. Conversely, the patients with tumors positive for both CD44v8-10 and SLX had a high recurrence rate and the poorest prognoses. In a multivariate analysis using the Cox regression model, the combined expression of SLX and CD44v8-10 emerged as an independent prognostic indicator. These results suggested that the combined expression of CD44v8-10 and SLX may be a biologic marker of prognostic significance.
authors.affiliation: First Department of Surgery, Fukui Medical University, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: T
authors.initials: T
authors.lastname: Goi
authors.forename: K
authors.initials: K
authors.lastname: Seki
authors.forename: N
authors.initials: N
authors.lastname: Ohtaki
authors.forename: M
authors.initials: M
authors.lastname: Maehara
authors.forename: T
authors.initials: T
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Niimoto
authors.forename: K
authors.initials: K
authors.lastname: Katayama
authors.forename: K
authors.initials: K
authors.lastname: Hirose
authors.forename: G
authors.initials: G
authors.lastname: Nakagawara
authors.forename: S
authors.initials: S
authors.lastname: Matsukawa
mesh_terms: D000911:Antibodies, Monoclonal; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D005260:Female; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D007150:Immunohistochemistry; D016256:Lewis X Antigen / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009844:Oligosaccharides / Q000378:metabolism*; D011379:Prognosis; D016016:Proportional Hazards Models; D000080506:Sialyl Lewis X Antigen; D016019:Survival Analysis
chemical_list: D000911:Antibodies, Monoclonal; D018960:Hyaluronan Receptors; D016256:Lewis X Antigen; D009844:Oligosaccharides; D000080506:Sialyl Lewis X Antigen