title: Cerebral excitatory amino acids and Na+,K+-ATPase activity during resuscitation of severely hypoxic newborn piglets.
abstract: We tested the hypothesis that early brain recovery in hypoxic newborn piglets is improved by resuscitating with an O2 supply close to the minimum level required by the newborn piglet brain. Severely hypoxic 2-5-d-old anaesthetized piglets were randomly divided into three resuscitation groups: hypoxaemic (n = 8), 21% O2 (n = 8), and 100% O2 groups (n = 8). The hypoxaemic group was mechanically ventilated with 12-18% O2 adjusted to achieve a cerebral venous O2 saturation of 17-23% (baseline; 45 +/- 1%, mean +/- SEM). During the 2h resuscitation period, extracellular aspartate and glutamate concentrations in the cerebral striatum were higher during hypoxaemic resuscitation (p = 0.044 and p = 0.055, respectively) than during resuscitation with 21% O2 or 100% O2, suggesting an unfavourable accumulation of potent excitotoxins during hypoxaemic resuscitation. The cell membrane Na+,K+-ATPase activity of cerebral cortical tissue after 2 h resuscitation was similar in the three groups (p = 0.30). In conclusion, hypoxaemic resuscitation did not normalize early cerebral metabolic recovery as efficiently as resuscitation with 21% O2 or 100% O2. Resuscitation with 21% O2 was as efficient as resuscitation with 100% O2 in this newborn piglet hypoxia model.
authors.affiliation: Department of Paediatric Research and Institute for Surgical Research, National Hospital, Oslo, Norway.
authors.forename: B A
authors.initials: BA
authors.lastname: Feet
authors.forename: E
authors.initials: E
authors.lastname: Gilland
authors.forename: F
authors.initials: F
authors.lastname: Groenendaal
authors.forename: N C
authors.initials: NC
authors.lastname: Brun
authors.forename: L
authors.initials: L
authors.lastname: Hellström-Westas
authors.forename: H
authors.initials: H
authors.lastname: Hagberg
authors.forename: O D
authors.initials: OD
authors.lastname: Saugstad
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000378:metabolism* / Q000628:therapy; D002540:Cerebral Cortex / Q000201:enzymology*; D002851:Chromatography, High Pressure Liquid; D018846:Excitatory Amino Acids / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D017072:Neostriatum / Q000378:metabolism*; D010102:Oxygen Inhalation Therapy; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013552:Swine
chemical_list: D018846:Excitatory Amino Acids; D000254:Sodium-Potassium-Exchanging ATPase