title: Fas (CD95)-transduced signal preferentially stimulates lupus peripheral T lymphocytes.
abstract: Fas (CD95) is a cell surface receptor whose biological function in circulating peripheral T cells is not well understood. To address the question of abnormal T cell sensitivity to Fas stimulation in systemic lupus erythematosus (SLE), we studied Fas-transduced stimulation and apoptosis in peripheral blood T cells from patients with SLE and normal control. Immobilized anti-Fas monoclonal antibodies (mAb) (imCH-11; IgM type) significantly stimulated SLE T cell proliferation compared to T cells from normal donors and patients with rheumatoid arthritis (p < 0.003 and p < 0.005, respectively). The soluble form of CH-11 and other immobilized anti-Fas mAb (UB-2, ZB-4; IgG type) failed to stimulate lupus T cells while immobilized human Fas ligand did. Furthermore, imCH-11 induced IL-2 and IL-6 mRNA expression. However, imCH-11 activation failed to induce expression of the T cell activation surface molecules CD25 and CD69. Addition of exogenous ceramide, a second messenger for Fas-mediated apoptosis signaling, also induced T cell proliferation in SLE and normal controls. Moreover, fumonisin B1, a specific ceramide synthase inhibitor, and caspase inhibitors markedly suppressed imCH-11 induced T cell proliferation, suggesting that the ceramide pathway may be involved in Fas-transduced stimulation signals in SLE T cells. These results show that SLE T cells have an alteration in the Fas signal transduction pathway leading to cell proliferation. This defect may be important in Fas-mediated peripheral immune homeostasis.
authors.affiliation: The University of Texas Health Science Center at San Antonio, Department of Medicine, 78284-7874, USA.
authors.forename: K
authors.initials: K
authors.lastname: Sakata
authors.forename: A
authors.initials: A
authors.lastname: Sakata
authors.forename: N
authors.initials: N
authors.lastname: Vela-Roch
authors.forename: R
authors.initials: R
authors.lastname: Espinosa
authors.forename: A
authors.initials: A
authors.lastname: Escalante
authors.forename: L
authors.initials: L
authors.lastname: Kong
authors.forename: T
authors.initials: T
authors.lastname: Nakabayashi
authors.forename: J
authors.initials: J
authors.lastname: Cheng
authors.forename: N
authors.initials: N
authors.lastname: Talal
authors.forename: H
authors.initials: H
authors.lastname: Dang
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000276:immunology; D000945:Antigens, Differentiation, T-Lymphocyte / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D037181:Lectins, C-Type; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000276:immunology; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D019014:fas Receptor / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D037181:Lectins, C-Type; D015375:Receptors, Interleukin-2; D019014:fas Receptor; D000099192:CD69 Antigens