Dendritic cells derived from bone marrow and CD34+ selected blood progenitor cells of myeloma patients, cultured in serum-free media, do not contain the Kaposi sarcoma herpesvirus genome.
The aim of our study was to test if dendritic cells contain the KSHV genome. CD34+ peripheral blood progenitor cells (PBPC) and bone marrow mononuclear cells were cultured in X-VIVO 15 medium supplemented with GM-CSF and TNF-alpha in gas-permeable containers. Dendritic cells were identified morphologically and immunophenotypically. The KSHV genome was not identified in any of the cases using a nested primer PCR approach. Serological analysis corroborated the molecular findings: no antibodies for KSHV were found in any of the multiple myeloma patients. These data are of importance when considering use of DC for therapeutic approaches in multiple myeloma.
Mitterer
M
M
Department of Haematology and Bone Marrow Transplantation Centre, Bozen/Bolzano, Italy.
Mair
W
W
Gatti
D
D
Sheldon
J
J
Vachula
M
M
Coser
P
P
Schultz
T F
TF
D018952:Antigens, CD34; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D003713:Dendritic Cells / Q000821:virology*; D016679:Genome, Viral; D019288:Herpesvirus 8, Human / Q000302:isolation & purification*; D006801:Humans; D009101:Multiple Myeloma / Q000821:virology*; D013234:Stem Cells / Q000821:virology
D018952:Antigens, CD34; D016895:Culture Media, Serum-Free