Protection of mice against lethal coxsackievirus B3 infection by using DNA immunization.
Vaccination with DNA and recombinant vaccinia viruses (rec.VV) has been studied with the coxsackievirus B3 (CVB3) model system. Plasmids encoding all structural proteins of CVB3, when injected intramuscularly, induced only low levels of virus-specific antibodies. However, DNA vaccination with the major structural protein VP1 protected 72.2% of mice from lethal challenge, whereas VP1 expressed by rec.VV was much less efficient.
Henke
A
A
Institute of Virology, Medical Center, Friedrich Schiller University, 07745 Jena, Germany.
Wagner
E
E
Whitton
J L
JL
Zell
R
R
Stelzner
A
A
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D003384:Coxsackievirus Infections / Q000517:prevention & control* / Q000821:virology; D029822:Enterovirus B, Human / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000627:therapeutic use*; D014616:Vaccinia virus / Q000235:genetics; D015678:Viral Structural Proteins / Q000235:genetics
D000914:Antibodies, Viral; D019444:Vaccines, DNA; D015678:Viral Structural Proteins