title: Several novel transcripts of glyceraldehyde-3-phosphate dehydrogenase expressed in adult chicken testis.
abstract: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), in addition to being a classic glycolytic enzyme, is a multifunctional protein involved in relevant cell functions such as DNA replication, DNA repair, translational control of gene expression, and apoptosis. Although the multifunctional nature of GAPDH suggests versatility in the mechanisms regulating its expression, no major qualitative changes and few quantitative changes in the GAPDH transcripts have been reported. While studying the expression of GAPDH during spermatogenesis, we detected alternative initiations to TATA box and alternative splicings in the 5' region of the pre-mRNA, resulting in at least six different types of mRNAs. The amount and the polyadenylation of the GAPDH transcripts increased in mature testis in relation to immature testis and further increased when cell suspensions from mature testis were exposed to heat shock. These results suggest that alternative initiation, alternative splicing, and polyadenylation could provide the necessary versatility to the regulation of the expression of this multifunctional protein during spermatogenesis.
authors.affiliation: Laboratori de Genètica Molecular, Institut d'Investigacions Biomèdiques August Pi Sunyer, Facultat de Medicina, Universitat de Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Mezquita
authors.forename: M
authors.initials: M
authors.lastname: Pau
authors.forename: C
authors.initials: C
authors.lastname: Mezquita
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics* / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D008297:Male; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010446:Peptide Fragments / Q000235:genetics* / Q000378:metabolism*; D011061:Poly A / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics; D016385:TATA Box; D013737:Testis / Q000254:growth & development / Q000378:metabolism*; D014018:Tissue Distribution; D014158:Transcription, Genetic
chemical_list: D018840:HSP70 Heat-Shock Proteins; D010446:Peptide Fragments; C068142:glyceraldehyde 3-phosphate dehydrogenase (304-313); D011061:Poly A; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases