Effect of superoxide and superoxide-generating systems on the prooxidant effect of iron in oil emulsion and raw turkey homogenates.
Mechanisms of superoxide.O2--generating systems on the pro-oxidant effect of iron from various sources were studied. Reaction mixtures were prepared with distilled water, oil emulsion, or meat homogenates. Free ionic iron (ferrous and ferric), ferritin and hemoglobin (Hb) were used as iron sources, and KO2 and xanthine oxidase (XOD) systems were used to produce .O2-. Thiobarbituric acid reactive substances (TBARS) values and iron contents of the reaction mixtures were determined. Ferric iron and ferritin, in the presence or absence of superoxide-generating systems, had no catalytic effect on the oxidation of oil emulsion but became pro-oxidants when reducing agent (ascorbate) was present. Ferrous iron and Hb had strong catalytic effects on the oxidation of oil emulsion as shown by TBARS values. Superoxide and H2O2, generated from superoxide-generating systems, oxidized ferrous iron and ascorbate, and lowered the pro-oxidant effect of ferrous iron in oil emulsion. Addition of ferric or ferrous iron increased but Hb did not have any effect on the TBARS values of raw meat homogenates. The reaction mechanisms of superoxide and the superoxide-generating systems on the prooxidant effect of various iron sources indicated that .O2- was a strong oxidizer rather than a reducing agent, and the antioxidant effect of XOD system in oil was caused by the oxidation of ferrous iron to the ferric form by .O2- and/or H2O2.
Ahn
D U
DU
Animal Science Department, Iowa State University, Ames 50011, USA.
Kim
S M
SM
D000818:Animals; D000975:Antioxidants; D004655:Emulsions; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology*; D015227:Lipid Peroxidation; D016877:Oxidants / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010938:Plant Oils; D011202:Poultry Products; D013481:Superoxides / Q000378:metabolism* / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014422:Turkeys; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D004655:Emulsions; D016877:Oxidants; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D013481:Superoxides; D006861:Hydrogen Peroxide; D007501:Iron; D014969:Xanthine Oxidase