Estimation of apoptosis in C6 glioma cells treated with antidepressants.
Gel electrophoresis of DNA was used for estimation of DNA changes caused in C6 glioma cells by treatment with psychotropic drugs (imipramine, amitryptiline and fluoxetine). Some discrete bands containing a population of short DNA fragments appeared after 1 and 5 days of cultivation. Apoptotic DNA breaks were verified at single cell level using the TUNEL test in cells treated with fluoxetine.
Spanová
A
A
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno.
Kovárů
H
H
Lisá
V
V
Lukásová
E
E
Rittich
B
B
D000639:Amitriptyline / Q000494:pharmacology; D000928:Antidepressive Agents / Q000494:pharmacology*; D017209:Apoptosis; D053938:DNA Fragmentation / Q000187:drug effects; D005473:Fluoxetine / Q000494:pharmacology; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007099:Imipramine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D000928:Antidepressive Agents; D005473:Fluoxetine; D000639:Amitriptyline; D007099:Imipramine