title: Intracellular cytokine expression in the CD4+ and CD8+ T cells from intestinal mucosa of simian immunodeficiency virus infected macaques.
abstract: Isolated intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) from jejunum of SIV infected animals were examined for alterations in basal cytokine expression by RT-PCR. Remarkable changes in IFNgamma and IL-10 RNA levels were observed in IEL and LPL in SIV infection while IL-4 and IL-2 RNA levels remained unaltered. In addition, the CD4+ and CD8+ LPL were examined for intracellular cytokine production following mitogenic activation by flowcytometry. Both CD4+ and CD8+ T lymphocytes in intestinal mucosa retained the potential to produce IFNgamma in response to mitogenic stimulation in vitro, without a remarkable change in IL-4 production. The dominant IFNgamma cytokine response could be one of the major contributing factors in SIV associated enteropathy.
authors.affiliation: Department of Internal Medicine, University of California at Davis 95616, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Smit-McBride
authors.forename: J J
authors.initials: JJ
authors.lastname: Mattapallil
authors.forename: F
authors.initials: F
authors.lastname: Villinger
authors.forename: A A
authors.initials: AA
authors.lastname: Ansari
authors.forename: S
authors.initials: S
authors.lastname: Dandekar
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics*; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D017403:In Situ Hybridization; D007413:Intestinal Mucosa / Q000276:immunology* / Q000821:virology; D007583:Jejunum / Q000276:immunology / Q000821:virology; D008253:Macaca mulatta; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology*; D015302:Simian Immunodeficiency Virus / Q000302:isolation & purification*
chemical_list: D016207:Cytokines