Effects of the garlic components diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity in human colon tumour cells.
Diallyl sulfide (DAS) and diallyl disulfide (DADS), major components of garlic, were used to determine inhibition of arylamine N-acetyltransferase (NAT) activity in a human colon tumour (adenocarcinoma) cell line. Two assay systems were performed, one with cellular cytosols (9000g supernatant), the other with intact bacterial cell suspensions. The NAT activity in a human colon tumour cell line was inhibited by DAS and DADS in a dose-dependent manner in both system: that is, the greater the concentration of DAS and DADS in the reaction, the greater the inhibition of NAT activities in both systems. The data also indicated that DAS and DADS decrease the apparent values of Km and Vmax of NAT enzymes from human colon tumour cells in both systems examined. This is the first report to demonstrate that garlic components do affect human colon tumour cell NAT activity.
Chen
G W
GW
Department of Surgery, Institute of Chinese Medical Science, China Medical College, Taichung, Taiwan, ROC.
Chung
J G
JG
Hsieh
C L
CL
Lin
J G
JG
D015073:2-Acetylaminofluorene / Q000378:metabolism; D010129:4-Aminobenzoic Acid / Q000378:metabolism; D000230:Adenocarcinoma / Q000201:enzymology*; D000498:Allyl Compounds / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001191:Arylamine N-Acetyltransferase / Q000037:antagonists & inhibitors*; D003110:Colonic Neoplasms / Q000201:enzymology*; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D004220:Disulfides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005737:Garlic; D006801:Humans; D007700:Kinetics; D010946:Plants, Medicinal; D013440:Sulfides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000498:Allyl Compounds; D000970:Antineoplastic Agents; D004220:Disulfides; D004791:Enzyme Inhibitors; D013440:Sulfides; C028009:diallyl disulfide; C038491:allyl sulfide; D015073:2-Acetylaminofluorene; D001191:Arylamine N-Acetyltransferase; D010129:4-Aminobenzoic Acid