Time-dependent expression of interleukin-10 (IL-10) mRNA during the early phase of skin wound healing as a possible indicator of wound vitality.
This study was performed to clarify whether interleukin-10 messenger RNA (IL-10 mRNA) could be a possible indicator for the distinction between intravital wounds and postmortem damage. After incision, mice were sacrificed from 0 to 180 min. The initial amount of IL-10 mRNA in each skin specimen was evaluated using the reverse transcriptase-polymerase chain reaction (RT-PCR). After 15 min there was a rapid increase in IL-10 mRNA which peaked at 60 min. A significant increase in IL-10 mRNA occurred between 30 and 180 min. During the 5 day postmortem interval the increase in time-dependent IL-10 mRNA expression was maintained and no significant increase in IL-10 mRNA expression occurred in the postmortem control. The increased expression of IL-10 mRNA could be considered a vital reaction in skin specimens with postmortem change. This study demonstrated the possible use of mRNA analysis for forensic wound examination because mRNA was detectable by RT-PCR over a longer postmortem time course.
Ohshima
T
T
Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Japan.
Sato
Y
Y
D000818:Animals; D016753:Interleukin-10 / Q000096:biosynthesis*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011180:Postmortem Changes; D012333:RNA, Messenger / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000293:injuries* / Q000378:metabolism; D013997:Time Factors; D014945:Wound Healing
D012333:RNA, Messenger; D016753:Interleukin-10