Gene organization and transcriptional analysis of the Spiroplasma citri rpsB/tsf/x operon.
The nucleotide sequence of a 6863-bp Spiroplasma citri DNA fragment comprising the spiralin gene was determined. Sequence analysis revealed eight putative ORFs that encode ribosomal protein S2, elongation factor Ts, spiralin, 6-phosphofructokinase, pyruvate kinase, and three unidentified proteins (A, B, and X). The gene organization reported here is different from that previously published. Northern blot analysis of rpsB, tsf, and x transcripts indicates that these genes are organized into a single transcriptional unit (operon). However, the detection of an additional transcript corresponding to the rpsB gene alone suggests that a transcriptional mechanism should occur in the 3' region of the rpsB gene, allowing a conditional transcription termination.
Le Dantec
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Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique and Université Victor Segalen Bordeaux 2, Domaine de la Grande Ferrade, BP 81, 33883 Villenave d'Ornon Cedex, France.
Bové
J M
JM
Saillard
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C
D001426:Bacterial Proteins / Q000235:genetics; D015152:Blotting, Northern; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D009876:Operon; D010445:Peptide Elongation Factors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D012269:Ribosomal Proteins / Q000235:genetics; D013149:Spiroplasma / Q000235:genetics*
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010445:Peptide Elongation Factors; D012269:Ribosomal Proteins; C023512:elongation factor Ts; C030004:ribosomal protein S2