CMIX, a paired-type homeobox gene expressed before and during formation of the avian primitive streak.
We cloned a chicken homeobox gene closely related to the Xenopus Mix. 1 gene. CMIX is expressed early in embryogenis in a sickle-shaped area in the posterior zone of the blastoderm. With the beginning of gastrulation, CMIX transcripts are found in the primitive streak primordium, then in the young and medium-sized streak, however not in the mesoderm after its emergence. In the fully-extended streak, CMIX is restricted to its middle, i.e. the prospective ventral mesoderm. CMIX RNA is undetectable by whole-mount in-situ analysis in later stages. We compare CMIX expression to the early pattern of the brachyury gene.
Stein
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S
Max-Planck-Institut für biophysikalische Chemie, Abteilung Molekulare Zellbiologie, Am Fassberg, D-37077, Göttingen, Germany.
Roeser
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T
Kessel
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M
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins; D002642:Chick Embryo; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism*; D047108:Embryonic Development; D005775:Gastrula / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D005801:Genes, Homeobox / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D012313:RNA / Q000032:analysis / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D030161:Avian Proteins; C116550:CMIX protein, chicken; D018076:DNA, Complementary; D018398:Homeodomain Proteins; D012313:RNA