title: Enhancement of phagocytosis in mouse peritoneal macrophages by fragments of acidic fibroblast growth factor (aFGF).
abstract: To characterize the effects of acidic fibroblast growth factor (aFGF) in mouse peritoneal macrophages, the effects of aFGF fragments on phagocytosis were examined. Fragments that were tested included aFGF(1-15), aFGF(1-20), aFGF(1-29), Ala16-aFGF(1-29), aFGF(9-29) and aFGF(114-140). aFGF(1-29) induced an enhancement of phagocytosis in a dose-dependent manner and was more effective than any other fragments tested. Even in Ca2+-and Mg2+-free solutions, phagocytosis was enhanced by aFGF(1-29). However, the enhancement induced by aFGF(1-29) was completely inhibited in the presence of mannan (4 mg/ml). Furthermore, the enhancement of phagocytosis by aFGF(1-29) was suppressed by heparin (100 microg/ml). The results of the present study suggest that the active region of aFGF that is responsible for the enhancement of phagocytosis corresponds to residues 15-29 and that phagocytosis, which is modulated by aFGF, is independent of extracellular Ca2+ and is mediated by mannose receptors.
authors.affiliation: Department of Physiology, Shimane Medical University, Izumo, Japan. michinos@shimane-med.ac.jp
authors.forename: M
authors.initials: M
authors.lastname: Ichinose
authors.forename: M
authors.initials: M
authors.lastname: Sawada
authors.forename: K
authors.initials: K
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Oomura
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D005434:Flow Cytometry; D006493:Heparin / Q000494:pharmacology; D037181:Lectins, C-Type; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology
chemical_list: D000925:Anticoagulants; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D016220:Fibroblast Growth Factor 1; D006493:Heparin; D002118:Calcium