Gene gun-mediated in vivo analysis of tissue-specific repression of gene transcription driven by the chicken ovalbumin promoter in the liver and oviduct of laying hens.
In order to search tissue-specific elements in the 5'-upstream promoter region, gene gun was used to transfect in vivo plasmid DNAs with varying lengths of truncated ovalbumin promoter fused to the CAT reporter gene to the oviduct and liver of laying hens. The results indicated that in the oviduct, consistently high reporter gene expression was observed irrespective of the length of the truncated ovalbumin gene promoters, whereas in the liver the ovalbumin promoter extending from -3200 to +8 bp suppressed substantially the reporter gene expression compared with consistently high gene expression obtained by the ovalbumin promoters from -2800 to +8 bp or shorter length. It was concluded, therefore, that a tissue-specific silencer-like element might reside most likely in the ovalbumin gene promoter region between -3200 and -2800 bp which represses the ovalbumin gene transcription in the liver, but not in the oviduct of laying hens.
Muramatsu
T
T
Laboratory of Department of Biological Resources and Environmental Sciences, School of Agricultural Sciences, Nagoya University, Japan.
Imai
T
T
Park
H M
HM
Watanabe
H
H
Nakamura
A
A
Okumura
J
J
D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002645:Chickens; D004274:DNA, Recombinant / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics; D018014:Gene Transfer Techniques; D017930:Genes, Reporter / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D010047:Ovalbumin / Q000235:genetics*; D010057:Oviducts / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D014158:Transcription, Genetic / Q000235:genetics*
D020121:5' Untranslated Regions; D004274:DNA, Recombinant; D010047:Ovalbumin