title: Modification of the in vivo four-point loading model for studying mechanically induced bone adaptation.
abstract: We modified the noninvasive, in vivo technique for strain application in the tibiae of rats (Turner et al., Bone 12:73-79, 1991). The original model applies four-point bending to right tibiae via an open-loop, stepper-motor-driven spring linkage. Depending on the magnitude of applied load, the model produces new bone formation at periosteal (Ps) or endocortical surfaces (Ec.S). Due to the spring linkage, however, the range of frequencies at which loads can be applied is limited. The modified system replaces this design with an electromagnetic vibrator. A load transducer in series with the loading points allows calibration, the loaders' position to be adjusted, and cyclic loading completed under load control as a closed servo-loop. Two experiments were conducted to validate the modified system: (1) a strain gauge was applied to the lateral surface of the right tibia of 5 adult female rats and strains measured at applied loads from 10 to 60 N; and (2) the bone formation response was determined in 28 adult female Sprague-Dawley rats. Loading was applied as a haversine wave with a frequency of 2 Hz for 18 sec, every second day for 10 days. Peak bending loads were applied at 33, 40, 52, and 64 N, and a sham-loading group was included at 64 N. Strains in the tibiae were linear between 10 and 60 N, and the average peak strain at the Ps.S at 60 N was 2664 +/- 250 microstrain, consistent with the results of Turner's group. Lamellar bone formation was stimulated at the Ec.S by applied bending, but not by sham loading. Bending strains above a loading threshold of 40 N increased Ec lamellar bone formation rate, bone forming surface, and mineral apposition rate with a dose response similar to that reported by Turner et al. (J Bone Miner Res 9:87-97, 1994). We conclude that the modified loading system offers precision for applied loads of between 0 and 70 N, versatility in the selection of loading rates up to 20 Hz, and a reproducible bone formation response in the rat tibia. Adjustment of the loader also enables study of mechanical usage in murine tibia, an advantage with respect to the increasing variety of transgenic strains available in bone and mineral research.
authors.affiliation: Department of Anatomical Sciences, University of Queensland, Brisbane, Australia. m.forwood@mailbox.uq.edu.au
authors.forename: M R
authors.initials: MR
authors.lastname: Forwood
authors.forename: M B
authors.initials: MB
authors.lastname: Bennett
authors.forename: A R
authors.initials: AR
authors.lastname: Blowers
authors.forename: R L
authors.initials: RL
authors.lastname: Nadorfi
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D001846:Bone Development / Q000502:physiology*; D005260:Female; D008954:Models, Biological; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013180:Sprains and Strains / Q000209:etiology / Q000503:physiopathology; D013977:Tibia / Q000502:physiology*; D016474:Weight-Bearing