title: Discrimination of Bordetella parapertussis and Bordetella pertussis organisms from clinical isolates by PCR using biotin-labelled oligonucleotide probes.
abstract: A recently developed shared-primer polymerase chain reaction (PCR) was investigated, in an ongoing pertussis surveillance study for discrimination of Bordetella parapertussis and Bordetella pertussis organisms, by using specific biotin-labelled oligonucleotide probes. From a total of 132 samples, 83 were positive by the B. parapertussis specific probe, 33 were positive by the B. pertussis specific probe and 16 samples containing Hemophilus influenzae as a negative control were below threshold by both probes. The shared-primer PCR in combination with specific oligonucleotide probes provides a rapid, sensitive and specific molecular diagnostic tool for future surveillance studies. In addition, it may be used to further investigate whether B parapertussis antigens should be added to acellular pertussis vaccines to protect against B. parapertussis infections.
authors.affiliation: Kinderklinik Universitätsklinikum, Aachen, Germany. fmmueller@mail.uniwuerzburg.de
authors.forename: F M
authors.initials: FM
authors.lastname: Müller
authors.forename: U
authors.initials: U
authors.lastname: Heininger
authors.forename: N
authors.initials: N
authors.lastname: Schnitzler
authors.forename: P
authors.initials: P
authors.lastname: Kockelkorn
authors.forename: O
authors.initials: O
authors.lastname: Cloot
authors.forename: C
authors.initials: C
authors.lastname: Lorenz
authors.forename: G
authors.initials: G
authors.lastname: Haase
mesh_terms: D001710:Biotin; D001884:Bordetella / Q000235:genetics* / Q000302:isolation & purification*; D001885:Bordetella Infections / Q000382:microbiology; D001886:Bordetella pertussis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity; D014917:Whooping Cough / Q000382:microbiology
chemical_list: D015345:Oligonucleotide Probes; D001710:Biotin