title: Comparative studies of class IIa bacteriocins of lactic acid bacteria.
abstract: Four class IIa bacteriocins (pediocin PA-1, enterocin A, sakacin P, and curvacin A) were purified to homogeneity and tested for activity toward a variety of indicator strains. Pediocin PA-1 and enterocin A inhibited more strains and had generally lower MICs than sakacin P and curvacin A. The antagonistic activity of pediocin-PA1 and enterocin A was much more sensitive to reduction of disulfide bonds than the antagonistic activity of sakacin P and curvacin A, suggesting that an extra disulfide bond that is present in the former two may contribute to their high levels of activity. The food pathogen Listeria monocytogenes was among the most sensitive indicator strains for all four bacteriocins. Enterocin A was most effective in inhibiting Listeria, having MICs in the range of 0.1 to 1 ng/ml. Sakacin P had the interesting property of being very active toward Listeria but not having concomitant high levels of activity toward lactic acid bacteria. Strains producing class IIa bacteriocins displayed various degrees of resistance toward noncognate class IIa bacteriocins; for the sakacin P producer, it was shown that this resistance is correlated with the expression of immunity genes. It is hypothesized that variation in the presence and/or expression of such immunity genes accounts in part for the remarkably large variation in bacteriocin sensitivity displayed by lactic acid bacteria.
authors.affiliation: Laboratory of Microbial Gene Technology, Department of Biotechnological Sciences, Agricultural University of Norway, N-1432 As, Norway. vincent.eijsink@ibf.nlh.no
authors.forename: V G
authors.initials: VG
authors.lastname: Eijsink
authors.forename: M
authors.initials: M
authors.lastname: Skeie
authors.forename: P H
authors.initials: PH
authors.lastname: Middelhoven
authors.forename: M B
authors.initials: MB
authors.lastname: Brurberg
authors.forename: I F
authors.initials: IF
authors.lastname: Nes
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001430:Bacteriocins / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology*; D003013:Clostridium / Q000187:drug effects; D004220:Disulfides / Q000737:chemistry; D016983:Enterococcus / Q000187:drug effects / Q000378:metabolism*; D007778:Lactobacillus / Q000187:drug effects / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010376:Pediococcus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; D004220:Disulfides