title: Chemiluminescent determination of esterases in monocytes.
abstract: Esterase from monocytes promotes the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) yielding 2-methyl-1-propenol, which is oxidized by horseradish peroxidase/H2O2 producing triplet acetone. The chemiluminescence of this reaction can be enhanced by the addition of 9,10-dibromoanthracene-2-sulphonate. The non-specific esterase present in monocytes is responsible for MPB hydrolysis, since (a) the chemiluminescence of the reaction was inhibited by fluoride, and (b) cells that do not contain a significant amount of non-specific esterases, e.g. lymphocytes and neutrophils, did not trigger light emission. The analytical application of this reaction is considered.
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
authors.forename: L M
authors.initials: LM
authors.lastname: da Fonseca
authors.forename: B
authors.initials: B
authors.lastname: Yavo
authors.forename: L H
authors.initials: LH
authors.lastname: Catalani
authors.forename: R P
authors.initials: RP
authors.lastname: Falcão
authors.forename: I L
authors.initials: IL
authors.lastname: Brunetti
authors.forename: A
authors.initials: A
authors.lastname: Campa
mesh_terms: D001565:Benzoates; D004950:Esterases / Q000097:blood*; D005456:Fluorescent Dyes; D006098:Granulocytes / Q000201:enzymology*; D006735:Horseradish Peroxidase; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology*
chemical_list: C101261:2-methyl-1-propenylbenzoate; D001565:Benzoates; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D004950:Esterases