Identification of sigma 32-like factors and ftsX-rpoH gene arrangements in enteric bacteria.
Western blot analyses using anti-Escherichia coli K-12 sigma 32 antibodies and Southern blot analyses using rpoH and ftsX DNA probes were performed using different enteric bacteria. Results show that the bacterial strains analysed have sigma 32-like transcription factors and ftsX and rpoH homologs in a similar map position. Although the presence of sigma 32-like factors seems to be extended to all Proteobacteria, rpoH and ftsX homologs seem to be present as neighbors in the genome only in the enteric bacteria.
Ramírez-Santos
J
J
Departamento de Biología Molecular, Universidad Nacional Autónoma de México, D.F., México.
Gómez-Eichelmann
M C
MC
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D015139:Blotting, Southern; D015153:Blotting, Western; D018797:Cell Cycle Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000302:isolation & purification; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D012808:Sigma Factor; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D018797:Cell Cycle Proteins; D004269:DNA, Bacterial; C102286:FtsX protein, bacteria; D006360:Heat-Shock Proteins; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32