Quantitative determination of (-)-2'-deoxy-3'-thiacytidine (lamivudine) in human plasma, saliva and cerebrospinal fluid by high-performance liquid chromatography with ultraviolet detection.
A high-performance liquid chromatographic method for the quantitative determination of the HIV reverse transcriptase inhibitor lamivudine ((-)-2'-deoxy-3'-thiacytidine, 3TC, Epivir) in human plasma, saliva and cerebrospinal fluid is described. Lamivudine was extracted from samples using silica extraction columns prior to reversed-phase high-performance liquid chromatography with ultraviolet detection at 270 nm. The method has been validated over the range of 10 (lower limit of quantitation) to 5000 ng/ml using a 0.5-ml sample volume. Between-day and within-day precisions ranged from 3.5 to 9.0%. The assay has been used for the quantitative analysis of lamivudine in plasma and cerebrospinal fluid of HIV-1 infected patients.
Hoetelmans
R M
RM
Department of Pharmacy & Pharmacology, Slotervaart Hospital, Amsterdam, The Netherlands.
Profijt
M
M
Mennhorst
P L
PL
Mulder
J W
JW
Beijnen
J H
JH
D019380:Anti-HIV Agents / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D015658:HIV Infections / Q000097:blood; D015497:HIV-1; D006801:Humans; D019259:Lamivudine / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D018894:Reverse Transcriptase Inhibitors / Q000032:analysis* / Q000097:blood / Q000134:cerebrospinal fluid / Q000493:pharmacokinetics; D012463:Saliva / Q000737:chemistry / Q000821:virology; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D016781:Toxoplasmosis, Cerebral / Q000134:cerebrospinal fluid / Q000188:drug therapy
D019380:Anti-HIV Agents; D018894:Reverse Transcriptase Inhibitors; D019259:Lamivudine