Development of long PCR techniques to analyze deletion mutations of the human hprt gene.
DNA primers and reaction conditions for long polymerase chain reaction amplification (PCR) of portions of the human hprt gene are presented. Use of these primers with DNA from previously defined hprt deletion mutants demonstrated production of the expected smaller size products as compared to DNA from wild type cells. These primers will be of value in the rapid analysis and subsequent sequencing of hprt deletion mutations in human cells.
Van Houten
B
B
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555, USA.
Chen
Y
Y
Nicklas
J A
JA
Rainville
I R
IR
O'Neill
J P
JP
D001483:Base Sequence; D002874:Chromosome Mapping; D004252:DNA Mutational Analysis / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D017384:Sequence Deletion
D017931:DNA Primers; D007041:Hypoxanthine Phosphoribosyltransferase