[Morphofunctional state of ischemically damaged kidney transplant in preservation and reperfusion (an experimental study)].
In cadaveric renal transplantation with simple cold storage, pretransplant evaluation of allograft viability creates an important problem. To assess the degree of ischemic damage and microcirculation of the renal allograft, experiment comparing parameters, like median perfusate flow and median intrarenal resistance, with histological findings was made. Kidney harvesting was performed from three groups of New Zealand rabbits by keeping warm ischemic time zero, thirty, sixty minutes for each group. Following perfusion with Euro-Collins solution and 24 and 48 hours of hypothermic preservation, perfusion was repeated. Perfusion parameters were measured and histopathological examination of the kidneys was made with light microscopy. In arterioles and glomerular, peritubular capillaries, red blood cell trapping was seen. It was also observed that an increase of warm ischemic time aggravates the erythrocyte aggregation and tubular degeneration. Following reperfusion, resistance measured from renal arteries by Protocol Propaq 106 manometer was named as terminal intrarenal resistance. It is noticeable that this simply measured parameter shows correlation with other parameters and histopathological findings.
Bagirov
A M
AM
D000818:Animals; D015925:Cryopreservation; D016030:Kidney Transplantation / Q000473:pathology* / Q000502:physiology; D008833:Microcirculation; D009926:Organ Preservation; D011817:Rabbits; D012079:Renal Circulation; D015427:Reperfusion Injury; D013997:Time Factors