Presence of dioxins in human follicular fluid: their possible stage-specific action on the development of preimplantation mouse embryos.
Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations between 0.5 and 100 pM and evaluated at 24-h intervals for their development to the eight-cell and blastocyst stages. The percentage of eight-cell embryos exposed to TCDD at 1, 2, and 5 pM concentrations was significantly lower than that of controls. However, blastocyst formation of the surviving eight-cell embryos was accelerated, with the number of cells in the blastocysts increased in a dose-dependent manner. Findings suggest that PCDDs and PCDFs may be present in human reproductive fluid and may exert some stage-specific effects on early embryonic development.
Tsutsumi
O
O
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan. osamut-tky@umin.ac.jp
Uechi
H
H
Sone
H
H
Yonemoto
J
J
Takai
Y
Y
Momoeda
M
M
Tohyama
C
C
Hashimoto
S
S
Morita
M
M
Taketani
Y
Y
D000818:Animals; D001755:Blastocyst / Q000187:drug effects*; D004147:Dioxins / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism*; D006801:Humans; D051379:Mice
D004147:Dioxins