Overexpression of Bcl-2 in Kaposi's sarcoma-derived cells.
The pathogenesis of Kaposi's sarcoma (KS), a tumor of probable vascular origin, remains an enigma. It is still unclear whether KS is a true malignancy or whether it represents a reactive polyclonal process. Using both an immunohistochemical and an immunoblot approach, we found that cells derived from KS lesions express significant levels of Bcl-2, a protein known to prolong cellular viability and to antagonize apoptosis. Bcl-2 expression was found in AIDS-related KS-derived cells, as well as in cells derived from iatrogenic and sporadic KS, indicating that Bcl-2 upregulation may be important in the pathogenesis of KS regardless of its epidemiologic form. By contrast, fibroblasts and dermal microvascular endothelial, cells which are the probable vascular progenitors of KS cells, expressed low levels of Bcl-2. The expression of Bcl-2 in KS-derived cells was associated with a long-term survival in serum-deprived conditions, a situation that has been shown to induce apoptosis in various cell types. Incubation of fibroblasts or of dermal microvascular endothelial cells with KS cell-free supernatants did not enhance Bcl-2 expression, suggesting that Bcl-2 expression is not mediated by an agent released by KS cells. Analogously, KS supernatants failed to promote the viability of fibroblasts and of dermal microvascular endothelial cells cultured in serum-free conditions. Our findings suggest that the spindle cells derived from KS have a survival advantage and may adequately represent the tumor cells of KS.
Simonart
T
T
Department of Dermatology, Erasme University Hospital, Brussels, Belgium.
Degraef
C
C
Noel
J C
JC
Fokan
D
D
Zhou
L
L
Pradier
O
O
Ducarme
M
M
Schandene
L
L
Van Vooren
J P
JP
Parent
D
D
Heenen
M
M
D002470:Cell Survival / Q000502:physiology; D016895:Culture Media, Serum-Free; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D012514:Sarcoma, Kaposi / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D016895:Culture Media, Serum-Free; D019253:Proto-Oncogene Proteins c-bcl-2