The influence of intravenous infusions of glucose and amino acids of pancreatic enzyme and mucosal protein synthesis in human subjects.
BACKGROUND
Animal studies have shown that the synthesis and secretion of pancreatic enzymes and the turnover of mucosal proteins is strongly influenced by diet.


METHODS
To determine whether the absorbed products of digestion are responsible for these changes, we investigated in groups of five healthy volunteers, the effects of i.v. infusions of amino acids (0.08 g/kg/h) and glucose (0.3 g/kg/h) on pancreatic enzyme and mucosal protein synthesis. Proteins were labeled in vivo by a 4-hours i.v. infusion of 14C-leucine and the enteric infusion of 3H-leucine tracer, during simultaneous cholecystokinin stimulation and duodenal collection of secreted pancreatic enzymes. Labeling of mucosal proteins was measured by endoscopic biopsy.


RESULTS
The amino acid infusions elevated plasma amino acid levels, and the glucose infusions increased both glucose and insulin concentrations. The rates for amylase and trypsin secretion were significantly lower during the first 2 hours of glucose infusion and the rate of synthesis of trypsin was delayed by i.v. amino acid infusions from 52.1 +/- 4.1 to 77.6 +/- 8.5 minutes. Mucosal protein turnover rates were unaffected. 3H-labeling via the enteral route showed similar enzyme synthesis rates but variable mucosal incorporation rates.


CONCLUSIONS
I.v. nutrients do not appear to stimulate the synthesis of pancreatic and mucosal proteins in human subjects.
O'Keefe
S J
SJ
Gastrointestinal Clinic, Department of Medicine, Groote Schuur Hospital, University of Cape Town, South Africa.
Lemmer
E R
ER
Ogden
J M
JM
Winter
T
T
D000328:Adult; D000596:Amino Acids / Q000008:administration & dosage* / Q000097:blood; D000681:Amylases / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002250:Carbon Radioisotopes; D005753:Gastric Mucosa / Q000378:metabolism; D005947:Glucose / Q000008:administration & dosage*; D006801:Humans; D007262:Infusions, Intravenous; D007328:Insulin / Q000097:blood; D007413:Intestinal Mucosa / Q000378:metabolism; D007700:Kinetics; D007930:Leucine; D010179:Pancreas / Q000201:enzymology*; D014176:Protein Biosynthesis; D014316:Tritium; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D001786:Blood Glucose; D002250:Carbon Radioisotopes; D007328:Insulin; D014316:Tritium; D000681:Amylases; D014357:Trypsin; D007930:Leucine; D005947:Glucose