title: Adherence of Candida albicans to denture-base materials with different surface finishes.
abstract: OBJECTIVE
  To assess the in vitro adherence of Candida albicans to heat-cured hard and soft denture-base materials with varying surface roughness, and to observe the effect of a mixed salivary pellicle on candidal adhesion to these surfaces.
  
  
  METHODS
  In vitro adhesion assays on heat-cured acrylic resin (Trevalon), Molloplast B and Novus using the type strain of C. albicans (NCPF 3153A). Surfaces for the assays were prepared using clinically appropriate rotary instruments. Unstimulated, pooled and clarified whole saliva was used to assess its effect on adhesion.
  
  
  RESULTS
  Significantly greater adhesion of C. albicans to rough rather than smooth surfaces was found (P < 0.001), as well as increased adhesion to the machined soft lining materials compared with acrylic. Pre-coating denture-base materials with saliva reduced candidal adhesion on all materials.
  
  
  CONCLUSIONS
  Rough surfaces on denture-base materials promote the adhesion of C. albicans in vitro. However, saliva reduces adhesion of C. albicans and thus diminishes the effect of surface roughness and free surface energy differences between materials.
authors.affiliation: Department of Prosthetic Dentistry, UMDS, Guy's and St Thomas's Medical and Dental School, University of London, UK.
authors.forename: D R
authors.initials: DR
authors.lastname: Radford
authors.forename: S P
authors.initials: SP
authors.lastname: Sweet
authors.forename: S J
authors.initials: SJ
authors.lastname: Challacombe
authors.forename: J D
authors.initials: JD
authors.lastname: Walter
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000268:Adhesiveness; D000328:Adult; D001672:Biocompatible Materials / Q000737:chemistry; D002176:Candida albicans / Q000502:physiology*; D017554:Carbon Compounds, Inorganic; D003762:Dental Instruments; D003764:Dental Materials / Q000737:chemistry*; D044622:Dental Pellicle; D003819:Denture Bases / Q000382:microbiology*; D003822:Denture Liners / Q000382:microbiology; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D006801:Humans; D008297:Male; D008768:Methylmethacrylates / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D012463:Saliva / Q000502:physiology; D017655:Silicon Compounds; D012826:Silicone Elastomers / Q000737:chemistry; D013232:Steel; D013499:Surface Properties; D017973:Tungsten Compounds
chemical_list: D000180:Acrylic Resins; D001672:Biocompatible Materials; D017554:Carbon Compounds, Inorganic; D003764:Dental Materials; D004129:Dimethylpolysiloxanes; C020964:MDX 4-4210; D008768:Methylmethacrylates; D009943:Organophosphorus Compounds; D011108:Polymers; D017655:Silicon Compounds; D012826:Silicone Elastomers; D017973:Tungsten Compounds; C076789:polyphosphazene fluoroelastomer; C002802:tungsten carbide; D013232:Steel; C029883:Trevalon universal; C022088:silicon carbide