title: The role of T cells in polyethylene particulate induced inflammation.
abstract: OBJECTIVE
  To investigate the role of T lymphocytes in ultra-high molecular weight polyethylene (UHMWPE) induced inflammation in joint arthroplasty.
  
  
  METHODS
  We address the role of T cells in wear induced inflammation by injecting the knee joints of both immune competent rats and mice and severe combined immunodeficient (SCID) mice with UHMWPE. Histological and immunohistochemical analysis of the synovial tissues was compared. Interaction between human T cells and UHMWPE particles was examined in vitro using T cell activation assays.
  
  
  RESULTS
  Histological and immunohistochemical analysis of the knees of the immune competent animals showed significant UHMWPE induced inflammation. In contrast, the tissue in the SCID mice knee joints showed very little inflammatory response to UHMWPE despite phagocytosis of the particulate. Since the SCID mice have no functional T or B lymphocytes, it is highly likely that the lack of inflammation in knee joints may be due to the absence of mouse T cells, as the infiltration of T cells into the joint tissue may enhance the inflammatory response to UHMWPE particles. T cell activation assays showed that T cells were not directly activated by UHMWPE particles and the nature of the interaction was not revealed from these experiments.
  
  
  CONCLUSIONS
  Although T cells are not directly involved in UHMWPE particle induced inflammation, as shown by the T cell activation assays, the histological data from the mice studies clearly show differences in the amplitude of inflammation from animals with and without functional T cells. Our studies suggest that the T cells may enhance the inflammatory response due to a bystander effect. Since the macrophages upon ingestion of UHMWPE particles release several cytokines including tumor necrosis factor-alpha, interleukin 1, and IL-6, it is possible that T cells in the vicinity of these macrophages may become attracted to the knee joint and activated due to cytokine release.
authors.affiliation: Centre for Biomaterials, Department of Surgery, University of Toronto, Mount Sinai Hospital, Ontario, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Sandhu
authors.forename: J E
authors.initials: JE
authors.lastname: Waddell
authors.forename: M
authors.initials: M
authors.lastname: Henry
authors.forename: E L
authors.initials: EL
authors.lastname: Boynton
mesh_terms: D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D001178:Arthroplasty; D002455:Cell Division / Q000187:drug effects; D006651:Histocytochemistry; D006801:Humans; D007719:Knee Joint / Q000187:drug effects / Q000473:pathology / Q000601:surgery; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D016513:Mice, SCID; D011095:Polyethylenes / Q000009:adverse effects*; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000473:pathology
chemical_list: D011095:Polyethylenes; C111601:ultra-high molecular weight polyethylene