C-terminal domain of beta-1,3-glucanase H in Bacillus circulans IAM1165 has a role in binding to insoluble beta-1,3-glucan.
The deduced amino acid sequences of 72-kDa beta-1,3-glucanase from Bacillus circulans WL-12 (GIcA) and 91-kDa enzyme from B. circulans IAM1165 (BglH) are highly homologous, except that the latter has an additional long C-terminal region composed of 192 amino acid residues. Two mutant enzymes (BgIH deprived of the C-terminal region and GIcA with the C-terminal region added) were constructed. The enzymes possessing the C-terminal region bound more abundantly to pachyman (insoluble beta-1,3-glucan) and A.spergillus oryzae cell wall than those not possessing the region. This indicates that the C-terminal region participated in binding of the enzymes to insoluble beta-1,3-glucan.
Yamamoto
M
M
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan. myamamot@bio.titech.ac.jp
Ezure
T
T
Watanabe
T
T
Tanaka
H
H
Aono
R
R
D000595:Amino Acid Sequence; D001407:Bacillus / Q000201:enzymology*; D001665:Binding Sites; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D043326:Glucan 1,3-beta-Glucosidase; D005936:Glucans / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins; D012091:Repetitive Sequences, Nucleic Acid; D017385:Sequence Homology; D012995:Solubility; D013329:Structure-Activity Relationship; D013696:Temperature; D047071:beta-Glucans; D001617:beta-Glucosidase / Q000737:chemistry* / Q000378:metabolism*
D005936:Glucans; D010446:Peptide Fragments; D011994:Recombinant Proteins; D047071:beta-Glucans; C033363:beta-1,3-glucan; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase