Effect of gamma irradiation on chemical and biological properties of lipopolysaccharide from Salmonella typhimurium.
Lipopolysaccharide (LPS) from S. typhimurium on exposure to gamma-radiation resulted in decrease in toxicity and was less mitogenic, Silver stained profiles of irradiated LPS on polyacrylamide gels revealed complete loss of its heteropolysaccharides which was confirmed further by analysing lipid A and LPS from Salmonella minnesota Re mutants on SDS-PAGE. Glucosamine and 2-keto 3-deoxy-octonate(Kdo) contents were significantly decreased on treatment. Lipid A obtained by removal of heteropolysaccharides from LPS was less toxic on exposure to gamma radiations.
Naidu
M D
MD
Food Technology Division, Bhabha Atomic Research Centre, Mumbai, India.
Chander
R
R
Nair
P M
PM
D000818:Animals; D005720:Gamma Rays; D008070:Lipopolysaccharides / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000737:chemistry / Q000528:radiation effects*; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D008070:Lipopolysaccharides; D008934:Mitogens