[Initial aspects of a program for in vitro transfection of cytokine genes into LNCap tumor cells].
OBJECTIVE
To characterize the conditions and kinetics for in vitro growth of tumoral cells LNCaP.


METHODS
Determination of proliferation curves and time to cell duplication through spectrophotometry and manual cell counts in haemocytometer with and without foetal calf serum (FCS) challenge.


RESULTS
Data from the spectrophotometry and manual cell counts were correlated by mean of the equation through the number of cells = absorbance at 595 nm x 227,530-14,160. The correlation coefficient r was 0.99. Cell proliferation was higher in the presence of FCS (p > 0.001), the final culture density being 5.4 times higher than the baseline. Similarly, time to duplication in the presence of FCS was 36.4 hours.


CONCLUSIONS
This experiment allowed to establish the local conditions for growth of LNCaP cells. In contrast to the behaviour exhibited by other malignant cells in culture, LNCaP cells grow slowly and require the stimulus provided by FCS. Both aspect are of the highest significance when adopting the pre-established timetables and logistic to the actual laboratory operation.
Páez Borda
A
A
Unidad de Investigación Urológica, Hospital Universitario de Getafe, Madrid.
Angulo Frutos
J
J
Luján Galán
M
M
Llanes González
L
L
Berenguer Sánchez
A
A
D002452:Cell Count; D002455:Cell Division; D003470:Culture Media; D016207:Cytokines / Q000235:genetics; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000473:pathology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D003470:Culture Media; D016207:Cytokines