Annexin V expression and membrane vesiculation during activation of leukemic cell lines.
We investigated annexin V expression and membrane vesiculation during activation of four leukemic cell lines (U937, HL60, HEL, and CMK11-5) in order to determine whether annexin V had a role in the coagulation abnormalities related to malignancy. After stimulation by tissue plasminogen activator, binding of a monoclonal anti-annexin V antibody to U937 cells and HL60 cells increased in comparison with binding to control cells. Stimulation with thrombin or lipopolysaccharide also induced such an increase, but U46619 did not. Following activation of U937 and HL60 cells with thrombin and lipopolysaccharide, microparticle formation increased. Tissue plasminogen activator caused an increase of microparticles in U937 cells, but not HL60 cells. On the other hand, CMK11-5 and HEL cells did not show any increase of microparticles. These results suggest that some agonists can potently stimulate expression of prothrombinase
Xie
G L
GL
First Department of Internal Medicine, Kansai Medical University, Osaka, Japan.
Nomura
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Fukuhara
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D017304:Annexin A5 / Q000096:biosynthesis*; D017209:Apoptosis / Q000187:drug effects; D015415:Biomarkers / Q000032:analysis; D002457:Cell Extracts / Q000737:chemistry; D017077:Culture Media, Conditioned / Q000737:chemistry; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D013917:Thrombin / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure
D017304:Annexin A5; D015415:Biomarkers; D002457:Cell Extracts; D017077:Culture Media, Conditioned; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D013917:Thrombin; D010959:Tissue Plasminogen Activator