Differential turnover of syntaxin and SNAP-25 during synaptogenesis in cultured cerebellar granule neurons.
In order to investigate the molecular mechanism underlying synaptogenesis, we examined the dynamics and stability of syntaxin 1A and SNAP-25 in cultured cerebellar granule cells. In neurons cultured for less than 5 days in vitro (DIV), syntaxin was highly expressed with a half-life of >48 hours. SNAP-25 was also expressed at 5 DIV, but at a lower level and with a much shorter half-life of 16 hours. As the neurons matured and established synpatic connections, the expression of both proteins increased steadily, with the more rapid increase between 5 DIV and 8 DIV associated with SNAP-25. The half-life of syntaxin was slightly increased in the mature neurons. SNAP-25, however, showed an increased half-life of about 35 hours. These results suggested that the dynamics and stability of the t-SNAREs are differentially modulated during synaptogenesis, which may be important in establishing and maintaining synaptic connections.
Sanders
J D
JD
Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Yang
Y
Y
Liu
Y
Y
D000818:Animals; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D006207:Half-Life; D008565:Membrane Proteins / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D050765:Qa-SNARE Proteins; D051381:Rats; D013569:Synapses / Q000378:metabolism / Q000502:physiology*; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; C494210:Snap25 protein, rat; C494213:Stx1a protein, rat; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1