title: Developmental study on reduction and kinks of the tail in a new mutant knotty-tail mouse.
abstract: The knotty-tail (knt/knt) mouse has a short and knotty tail. The tail deformity is caused by a decrease in the number of caudal vertebrae and a deformity of them in the distal part of the tail. The objective of the study was to determine how reduction and kinks of the tail region were formed during secondary body formation. By day 12.0 pc, the somitogenesis of knt/knt embryos was completed; the number of caudal somites more or less agreed with those of the caudal vertebrae in knt/knt mice and were similar to those of knt/+ embryos. On the other hand, the somitogenesis of knt/+ embryos continued up to day 12.5 pc. The somites below about the sixth caudal somite were wedge-shaped with a dorsal apex in knt/knt embryos. The location of abnormal somites also corresponded well to that of deformed caudal vertebrae. Abnormal somitogenesis was always preceded by abnormalities in the presomitic region. Under gross observation, this could be seen to become markedly thickened, and histologically its dorsoventral diameter increased in the transverse plane on days 10.5-12.0 pc. In the mesenchyme there was often obvious cell death at the boundary of the unsegmented area and the tail bud after day 10.5 pc. These results suggested that the shortness of tail was primarily caused by the agenesis of distal caudal vertebrae following the agenesis of distal caudal somites, and partly by the disappearance of the presomitic part due to cell death, while the tail kinks were caused by the deformation of each caudal vertebra following disturbances of the caudal somites. Also, it is highly probable that the prominent cell death at the boundary of the unsegmented area and the tail bud may involve a defect or deformity of somites in this mutant.
authors.affiliation: Research Institute of Drug Safety, Setsunan University, Hirakata, Osaka, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Matsuura
authors.forename: I
authors.initials: I
authors.lastname: Narama
authors.forename: K
authors.initials: K
authors.lastname: Ozaki
authors.forename: M
authors.initials: M
authors.lastname: Nishimura
authors.forename: T
authors.initials: T
authors.lastname: Imagawa
authors.forename: H
authors.initials: H
authors.lastname: Kitagawa
authors.forename: M
authors.initials: M
authors.lastname: Uehara
mesh_terms: D000818:Animals; D001947:Breeding; D004475:Ectoderm / Q000166:cytology; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008817:Mice, Mutant Strains / Q000002:abnormalities / Q000033:anatomy & histology* / Q000235:genetics; D009024:Morphogenesis; D009436:Neural Tube Defects / Q000235:genetics; D009672:Notochord / Q000002:abnormalities; D011247:Pregnancy; D019170:Somites / Q000166:cytology; D013131:Spine / Q000002:abnormalities* / Q000033:anatomy & histology; D013623:Tail / Q000002:abnormalities* / Q000033:anatomy & histology