title: Comparison of culture and acid-fast bacilli stain to PCR for detection of Mycobacterium tuberculosis in clinical samples.
abstract: The major drawback in effective use of polymerase chain reaction (PCR) for detecting Mycobacterium tuberculosis (MTB) in clinical samples is the presence of PCR inhibitors and unique cell components of the organism that complicate DNA extraction and subsequent PCR amplification. A PCR assay with a unique multistep DNA extraction method that minimizes these problems was compared in a prospective study to acid-fast bacilli stain (AFBS) and culture for detecting MTB in clinical samples. A total of 254 clinical specimens in two separate studies were processed for MTB by these techniques. While PCR and culture were 100% sensitive and specific, culture required up to 8 weeks of incubation and additional time to perform biochemical testing to identify the isolated micro-organism. Acid-fast bacilli stain had a specificity of about 87% and did not differentiate among Mycobacterial species. In contrast, the results from PCR were available within 48 h and did not require additional testing to attain a final result. Polymerase chain reaction was highly reliable for detection and confirmation and interpretation of positive AFBS results. The assay was easy to perform with a turn around time of about 2 days.
authors.affiliation: Department of Laboratory Medicine, University of Connecticut Health Center, Farmington 06030, USA.
authors.forename: J
authors.initials: J
authors.lastname: Aslanzadeh
authors.forename: M
authors.initials: M
authors.lastname: de la Viuda
authors.forename: M
authors.initials: M
authors.lastname: Fille
authors.forename: W B
authors.initials: WB
authors.lastname: Smith
authors.forename: H
authors.initials: H
authors.lastname: Namdari
mesh_terms: D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D008168:Lung / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D012189:Retrospective Studies; D013183:Sputum / Q000382:microbiology; D013194:Staining and Labeling / Q000379:methods; D014376:Tuberculosis / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial