4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide as a fluorescent labelling reagent for determination of carboxylic acids in precolumn high-performance liquid chromatography.
A fluorescent labelling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide, was designed for the determination of carboxylic acids by precolumn HPLC. The reagent was reacted with carboxylic acids at 100 degrees C for 15 min in the presence of an activating reagent (a mixture of triphenylphosphine, 2,2'-dipyridyldisulfide and pyridine) and produced highly fluorescent derivatives, which were separated on a reversed-phase column by fluorescence measurement at 317 nm (excitation) and 380 nm (emission). The detection limits were 4-12 fmol/injection. The reagent was used for HPLC assays of long chain fatty acids in human serum by isocratic elution.
Inoue
H
H
Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
Ikeno
M
M
Ishii
Y
Y
Tsuruta
Y
Y
D002264:Carboxylic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000097:blood; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D010797:Phthalimides / Q000138:chemical synthesis* / Q000494:pharmacology; D012997:Solvents; D013050:Spectrometry, Fluorescence
C093940:4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl chloride; D002264:Carboxylic Acids; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010797:Phthalimides; D012997:Solvents