Morphological effects of Pasteurella multocida type-D dermonecrotoxin on rat osteosarcoma cells in a nude mouse model.
Of 15 athymic nude mice that received subcutaneous implants of a rat osteosarcoma cell line, two groups of four subsequently received either a short (group 1) or a more prolonged (group 2) course of subcutaneous injections of the dermonecrotic toxin (DNT) of Pasteurella multocida type D. The remaining seven mice (controls) received no DNT. Both groups of DNT-treated mice lost body weight as compared with controls. Tumour weight, expressed as a percentage of body weight, increased in the four group 1 mice. Tumours in this group 1 were consistently larger than those in appropriate controls, indicating that this percentage was not simply a function of decreased body weight. The immunohistochemical labelling of proliferating cell nuclear antigen (PCNA) and morphometric analysis of intratumoral necrosis suggested that the DNT had a mitogenic effect and contributed to the neoplastic growth. The presence of foci of neoplastic osteoblasts in the lungs of some DNT-treated mice suggested that the enhanced tumour growth led to an increased incidence of metastasis.
Dyer
N W
NW
Veterinary Diagnostic Laboratory, North Dakota State University, Fargo 58105, USA.
Haynes
J S
JS
Ackermann
M R
MR
Rimler
R B
RB
D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D002452:Cell Count; D002455:Cell Division; D004273:DNA, Neoplasm / Q000187:drug effects; D007124:Immunoenzyme Techniques; D008175:Lung Neoplasms / Q000737:chemistry / Q000556:secondary*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000737:chemistry / Q000188:drug therapy / Q000556:secondary*; D016979:Pasteurella multocida; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis; D051381:Rats; D012878:Skin Neoplasms / Q000737:chemistry / Q000188:drug therapy / Q000473:pathology*; D014407:Tumor Cells, Cultured; D015431:Weight Loss / Q000187:drug effects
D001427:Bacterial Toxins; D004273:DNA, Neoplasm; D018809:Proliferating Cell Nuclear Antigen