Induction of cyclooxygenase (COX)-2 but not COX-1 gene expression in apoptotic cell death.
In this study we assessed the regulation of cyclooxygenase (COX)-2 in models of apoptotic cell death in vivo and in vitro. By 6 h after hippocampal colchicine injection in rat, COX-2 (but not COX-1) mRNA expression was elevated. The induction of COX-2 mRNA expression preceded temporally and overlapped anatomically the cellular morphological features of apoptosis in the granule cell layer of the dentate gyrus. Similarly, in an established in vitro model of apoptosis in P19 cells, COX-2 induction preceded apoptosis in response to serum deprivation by 12 h. These studies suggest that COX-2 may be involved in the early mechanisms leading to apoptosis.
Ho
L
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Neuroinflammation Research Centre, Department of Psychiatry, The Mount Sinai School of Medicine, New York, NY 10029-6574, USA.
Osaka
H
H
Aisen
P S
PS
Pasinetti
G M
GM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D001798:Blood Proteins / Q000494:pharmacology; D003078:Colchicine; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D018891:Dentate Gyrus / Q000201:enzymology / Q000276:immunology; D015971:Gene Expression Regulation, Enzymologic / Q000276:immunology*; D007527:Isoenzymes / Q000032:analysis / Q000235:genetics*; D008297:Male; D008565:Membrane Proteins; D014411:Neoplastic Stem Cells; D009410:Nerve Degeneration / Q000139:chemically induced / Q000201:enzymology / Q000276:immunology; D010544:Peroxidases / Q000032:analysis / Q000235:genetics; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000235:genetics*; D017966:Pyramidal Cells / Q000201:enzymology / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D001798:Blood Proteins; D007527:Isoenzymes; D008565:Membrane Proteins; D012333:RNA, Messenger; D010544:Peroxidases; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; C496513:Ptgs1 protein, rat; D003078:Colchicine