title: Toxoplasma gondii: an ultrastructural study of host-cell invasion by the bradyzoite stage.
abstract: The invasion of Toxoplasma gondii tachyzoites and bradyzoites was followed in bovine kidney cells via electron microscopy. The process of invasion differed between bradyzoites and tachyzoites. In the early stages of entry there was evidence of localised formation of membrane projections in the host cell adjacent to the parasite. Parasite reorientation and rhoptry release appeared to be necessary for invasion; however, the tight junction could not be clearly discerned and there was no evidence of constriction or of any membrane shedding from the parasite. The resulting parasitophorous vacuole was smaller than the tachyzoite vacuole and parasites were frequently found to lie immediately under the host cell membrane. The vacuole was rapidly adapted by the release and formation of an intra-phagosomal membrane network, while the parasitophorous vacuole formed a relationship with host-cell endoplasmic reticulum.
authors.affiliation: Department of Biology, University of Leeds, UK. PABJES@lucs-mac.novell.leeds.ac.uk
authors.forename: P M
authors.initials: PM
authors.lastname: Sasono
authors.forename: J E
authors.initials: JE
authors.lastname: Smith
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000469:parasitology* / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000469:parasitology / Q000648:ultrastructure; D008854:Microscopy, Electron; D014122:Toxoplasma / Q000254:growth & development / Q000472:pathogenicity* / Q000648:ultrastructure*; D014617:Vacuoles / Q000648:ultrastructure