NF-kappa B activation following optic nerve transection.
In order to elucidate in vivo neuronal cell death in the retina, and involvement of NF-kappa B in this process, we studied the degeneration of retinal ganglion cells (RGCs) and the activation of NF-kappa B after transection of the optic nerve of adult rat at 5 mm from the eyeball. The morphology of dying ganglion cells in the retinal ganglion cell layer was observed by light and electron microscopy, the activation of NF-kappa B was investigated immunohistochemically. Seven and 14 days post-axotomy, dying cells contained pyknotic nuclei. The death of retinal ganglion cells involved apoptosis, activation of NF-kappa B (p50 and p65) was prominent in a time dependent manner. We observed axotomy-induced NF-kappa B activation, which may mediate apoptosis of retinal ganglion cells.
Choi
J S
JS
Department of Ophthalmology, College of Medicine, Catholic University, Seoul, Korea.
Sungjoo
K Y
KY
Joo
C K
CK
D000818:Animals; D017209:Apoptosis / Q000502:physiology; D019771:Axotomy; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D016328:NF-kappa B / Q000096:biosynthesis*; D009900:Optic Nerve / Q000601:surgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure
D016328:NF-kappa B