title: The iridoid glucoside secologanin is derived from the novel triose phosphate/pyruvate pathway in a Catharanthus roseus cell culture.
abstract: Secologanin is the iridoid building block of the majority of the terpenoid indole alkaloids. In the biosynthesis of secologanin, mevalonate was considered to be the exclusive precursor of isopentenyl diphosphate. After [1-(13)C]glucose feeding to a cell culture of Catharanthus roseus, its incorporation into secologanin was studied by 13C NMR spectroscopy. The data showed that the novel triose phosphate/pyruvate and not the mevalonate pathway was the major route for the biosynthesis of secologanin.
authors.affiliation: Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, The Netherlands. contin_a@chem.leidenuniv.nl
authors.forename: A
authors.initials: A
authors.lastname: Contin
authors.forename: R
authors.initials: R
authors.lastname: van der Heijden
authors.forename: A W
authors.initials: AW
authors.lastname: Lefeber
authors.forename: R
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D002478:Cells, Cultured; D045782:Hemiterpenes; D057888:Iridoid Glucosides; D039823:Iridoids; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010944:Plants / Q000378:metabolism*; D011714:Pyrans / Q000737:chemistry / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*
chemical_list: D045782:Hemiterpenes; D057888:Iridoid Glucosides; D039823:Iridoids; D009943:Organophosphorus Compounds; D011714:Pyrans; D011773:Pyruvates; C021086:secologanin; C004809:isopentenyl pyrophosphate