Following the folding of RNA with time-resolved synchrotron X-ray footprinting.
The rapid mixing synchrotron X-ray footprinting technique described in this article allows nucleic acid folding and ligand binding reactions to be followed on a millisecond time resolution with single nucleotide resolution. In principle, the change in .OH protection of every nucleotide in a nucleic acid hundreds of nucleotides long can be monitored separately. In addition, a wide range of solution conditions are compatible with the radiolytic generation of .OH. These characteristics of synchrotron X-ray footprinting create opportunities for conducting thermodynamic and kinetic studies of nucleic acids that are both comprehensive and detailed. Kinetic footprinting studies of a number of systems have been initiated by the Center for Synchrotron Biosciences using this technique.
Sclavi
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B
Department of Physiology and Biophysics, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
Woodson
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S
Sullivan
M
M
Chance
M
M
Brenowitz
M
M
D017022:Flow Injection Analysis / Q000379:methods*; D017665:Hydroxyl Radical; D007700:Kinetics; D008024:Ligands; D008274:Magnesium / Q000494:pharmacology; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry* / Q000187:drug effects / Q000528:radiation effects; D017356:Synchrotrons; D014965:X-Rays
D008024:Ligands; D017665:Hydroxyl Radical; D012313:RNA; D008274:Magnesium