Effect of octreotide on the pathology of hepatic schistosomiasis.
In clinical practice, octreotide (CAS 83150-76-9) has its greatest impact in the management of bleeding varices. The present work is the first one which was undertaken to investigate the possible use of octreotide as an antifibrotic agent and to study its effect on hepatic vasculature in Schistosoma mansoni infection. The material of this investigation consisted of two groups of albino mice (A, B), subdivided each into normal control, infected control, subgroups treated with octreotide, praziquantel (CAS 55268-74-1), and a combination of octreotide and praziquantel. Groups A and B were sacrificed at the 8th week and the 18th week post infection, respectively. By analysis of the obtained results, octreotide induced a reduction of the portal pressure, the weight of the spleen and the liver, the liver egg load (number of eggs) granuloma size and cellularity, and of the degree of hepatic fibrosis quantified by serum N-terminal peptide of type III procollagen in serum, serum laminin and tissue collagen using a Picrosirius red dye assay. Moreover, the biochemical state of hepatocytes has been improved. The subgroups treated with octreotide in association with praziquantel revealed better results than the subgroups treated with praziquantel alone. These obtained data were analysed in terms of histological extent of liver fibrosis in sections stained with Masson trichrome and sirius red, hepatocytic and sinusoidal changes at an ultrastructural level and by immunohistochemical demarcation of endothelial cells of blood vessels through the determination of factor VIII-related antigen. The promising results detected in this study may encourage to further investigate the positive findings of this drug with the intention of its possible application on a clinical level.
Mansy
S S
SS
Department of Electron Microscopy, Theodor Bilharz Research Institute, Imbaba Guiza, Egypt.
Yehia
H A
HA
Hassan
M M
MM
Hassan
E A
EA
Youssef
M M
MM
Hadi
A A
AA
Mackenzie
C D
CD
D000818:Animals; D001808:Blood Vessels / Q000469:parasitology / Q000473:pathology; D003094:Collagen / Q000378:metabolism; D004357:Drug Synergism; D005260:Female; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000469:parasitology / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism / Q000469:parasitology / Q000473:pathology; D008111:Liver Function Tests; D051379:Mice; D008815:Mice, Inbred Strains; D015282:Octreotide / Q000494:pharmacology*; D011223:Praziquantel / Q000494:pharmacology; D012555:Schistosomiasis mansoni / Q000469:parasitology / Q000473:pathology*; D012556:Schistosomicides / Q000494:pharmacology
D012556:Schistosomicides; D011223:Praziquantel; D003094:Collagen; D015282:Octreotide