cDNA cloning and inducible expression of human multidrug resistance associated protein 3 (MRP3).
Previously, we cloned rat MRP3 as a candidate for an inducible transporter for the biliary excretion of organic anions [Hirohashi et al. (1998) Mol. Pharmacol. 53, 1068-10751. In the present study, we cloned human MRP3 (1527 amino acids) from Caco-2 cells. Human MRP3 is predominantly expressed in liver, small intestine and colon; hepatic expression of MRP3 was observed in humans but not in normal rats. In HepG2 cells, the expression of MRP3 was induced by phenobarbital. These results suggest that MRP3 may act as an inducible transporter in the biliary and intestinal excretion of organic anions.
Kiuchi
Y
Y
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Japan.
Suzuki
H
H
Hirohashi
T
T
Tyson
C A
CA
Sugiyama
Y
Y
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D018938:Caco-2 Cells; D006528:Carcinoma, Hepatocellular; D003001:Cloning, Molecular; D003106:Colon / Q000737:chemistry; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D007421:Intestine, Small / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008113:Liver Neoplasms; D008969:Molecular Sequence Data; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010634:Phenobarbital / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D018528:ATP-Binding Cassette Transporters; D018076:DNA, Complementary; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D010634:Phenobarbital; C114462:multidrug resistance-associated protein 3