title: Prenatal determination of fetal RhD (rhesus positive) type by an amplification of DNA.
abstract: Examination of RhD genotype (Rhesus D gene) from amniotic cells (or chorionic villi cells) by PCR amplification of DNA can be done at early stage of pregnancy. Due to some missing exons in the Rh partial D variant (e.g. DVI), it is necessary to use different PCR systems to get relevant results. The localization of primers in our three PCR systems is on the different exons (10, 7, and 4 + 5). The advantage of PCR technique is prenatal detection of RhD of fetus from nonerythrocytes suspension (e.g. from an amnion fluid cell sediment) in comparison to a "standard" haemagglutination serological technique which uses blood erythrocytes only. The possibility of this technique to distinguished the heterozygous (D/d) or homozygous (D/D) fathers can help the clinicians in decisions about the management of further prevention of the hemolytic disease of newborn.
authors.affiliation: Department of Clinical Haematology, General University Hospital, Prague, Czech Republic. kvasnic.@OKHVFN.anet.cz
authors.forename: J
authors.initials: J
authors.lastname: Kvasnicka
authors.forename: B
authors.initials: B
authors.lastname: Rypácková
authors.forename: P
authors.initials: P
authors.lastname: Calda
authors.forename: Z
authors.initials: Z
authors.lastname: Hájek
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D002824:Chorionic Villi / Q000737:chemistry; D005260:Female; D005312:Fetal Blood / Q000737:chemistry*; D006580:Genetic Carrier Screening; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012204:Rh-Hr Blood-Group System / Q000097:blood* / Q000235:genetics
chemical_list: D012204:Rh-Hr Blood-Group System