Reappraisal of the expression of mast cell proteases of Mongolian gerbils (Meriones unguiculatus).
Mast cell proteases in the tongue and jejunum of Mongolian gerbils (Meriones unguiculatus) were examined by enzyme-histochemical methods. Both trypsin-like (tryptase) and chymotrypsin-like (chymase) protease activities were demonstrated in mast cells in the tongue of fresh cryosections. When frozen sections of the tongue were post-fixed in various fixatives, those fixed in Carnoy's fluid showed strongest enzyme activities. Tryptase and chymase activities in paraffin sections of both tissues were well preserved when tissues were fixed in Carnoy's fluid at 4 degrees C for 15 min. However, enzyme activities in both tissues, especially in the tongue, were drastically reduced by longer fixation time and higher temperature. When Carnoy-fixed (4 degrees C for 15 min) paraffin sections were treated with heparinase I or chondroitinase ABC before enzyme-histochemical stainings for proteases, tryptase activities were lost entirely in the tongue and mostly in the jejunum by heparinase I digestion, and slightly in both organs by chondroitinase ABC digestion. In contrast, chymase activities at both sites were not influenced by these pretreatments. These results show that although mast cells in the tongue as well as in the jejunum of Mongolian gerbils contain both tryptase and chymase activities, their stability to fixations is variable among organs so that tissue fixation conditions are crucial for the preservation. At least some part of the stability of mast cell proteases is dependent on the proteoglycans present in mast cell granules.
Kurokawa
M
M
Department of Dermatology, Miyazaki Medical College, Japan.
Ogata
K
K
Uchiyama
F
F
Kobayashi
T
T
Ishiwata
K
K
Nawa
Y
Y
D000818:Animals; D019765:Chondroitin ABC Lyase / Q000494:pharmacology; D053818:Chymases; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability; D005629:Frozen Sections; D005849:Gerbillinae; D019763:Heparin Lyase / Q000494:pharmacology; D007583:Jejunum / Q000201:enzymology; D008407:Mast Cells / Q000201:enzymology*; D016612:Paraffin Embedding; D012697:Serine Endopeptidases / Q000032:analysis / Q000096:biosynthesis*; D014059:Tongue / Q000201:enzymology; D053802:Tryptases
D012697:Serine Endopeptidases; C083294:chymase 2; D053818:Chymases; D053802:Tryptases; D019765:Chondroitin ABC Lyase; D019763:Heparin Lyase