title: Equine CRISP-3: primary structure and expression in the male genital tract.
abstract: Although originally described in the male rodent genital tract, cysteine-rich secretory proteins (CRISPs) are expressed in a variety of mammalian tissue and cell types. The proteins of the male genital tract have been observed associated to spermatozoa and are believed to play a role in mammalian fertilization. Here we describe the identification and primary structure of the first equine member of the CRISP family. Equine CRISP-3 is transcribed and expressed in the stallion salivary gland, in the ampulla and the seminal vesicle. It displays all 16 conserved cysteine residues and shows 82% homology to human and 78% to guinea pig CRISP-2 (AA1, TPX 1) and 77% to human CRISP-3. In contrast to other mammalia, in the horse CRISP-3 is synthesized in great amounts in the accessory sexual glands, ampulla and seminal vesicle, thus allowing the isolation of equine CRISP-3 in amounts suitable for biochemical, physiological and structural studies from stallion seminal plasma.
authors.affiliation: Institut für Reproduktionsmedizin, Tierärztliche Hochschule, Bünteweg 15, D-30559 Hannover, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Schambony
authors.forename: M
authors.initials: M
authors.lastname: Gentzel
authors.forename: H
authors.initials: H
authors.lastname: Wolfes
authors.forename: M
authors.initials: M
authors.lastname: Raida
authors.forename: U
authors.initials: U
authors.lastname: Neumann
authors.forename: E
authors.initials: E
authors.lastname: Töpfer-Petersen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence / Q000235:genetics; D005786:Gene Expression Regulation / Q000235:genetics; D006736:Horses; D008297:Male; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000737:chemistry*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: C489926:CRISP3 protein, human; D010446:Peptide Fragments; D012333:RNA, Messenger; D012471:Salivary Proteins and Peptides; D029589:Seminal Plasma Proteins