title: Polarity, integrin, and extracellular matrix dynamics in the postischemic rat kidney.
abstract: Acute renal failure (ARF) as a consequence of ischemic injury is a common disease affecting 5% of the hospitalized population. Despite the fact that mortality from ARF is high, there has been little improvement in survival rates over the last 40 years. The pathogenesis of ARF may be related to substantial changes in cell-cell and cell-extracellular matrix interactions mediated by beta1-integrins. On the basis of in vitro and in vivo studies, reorganization of beta1-integrins from basal to apical surfaces of injured tubular epithelia has been suggested to facilitate epithelial detachment, contributing to tubular obstruction and backleak of glomerular filtrate. In this study, we examine integrin and extracellular matrix dynamics during epithelial injury and repair using an in vivo rat model of unilateral ischemia. We find that, soon after reperfusion, beta1-integrins newly appear on lateral borders in epithelial cells of the S3 segment but are not on the apical surface. At later times, as further injury and regeneration coordinately occur, epithelia adherent to the basement membrane localize beta1 predominantly to basal surfaces even while the polarity of other marker proteins is lost. At the same time, amorphous material consisting of depolarized exfoliated cells fills the luminal space. Notably, beta1-integrins are not detected on exfoliated cells. A novel finding is the presence of fibronectin, a glycoprotein of plasma and the renal interstitium, in tubular spaces of the distal nephron and to a lesser extent S3 segments. These results indicate that beta1-integrins dramatically change their distribution during ischemic injury and epithelial repair, possibly contributing to cell exfoliation initially and to epithelial regeneration at later stages. Together with the appearance of large amounts of fibronectin in tubular lumens, these alterations may play a significant role in the pathophysiology of ARF.
authors.affiliation: Renal Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA.
authors.forename: A
authors.initials: A
authors.lastname: Zuk
authors.forename: J V
authors.initials: JV
authors.lastname: Bonventre
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: K S
authors.initials: KS
authors.lastname: Matlin
mesh_terms: D058186:Acute Kidney Injury / Q000209:etiology / Q000503:physiopathology*; D000818:Animals; D002462:Cell Membrane / Q000473:pathology / Q000502:physiology; D016764:Cell Polarity; D005109:Extracellular Matrix / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000096:biosynthesis*; D019012:Integrin beta1 / Q000032:analysis / Q000378:metabolism*; D007511:Ischemia / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D007668:Kidney / Q000098:blood supply* / Q000473:pathology; D007679:Kidney Medulla / Q000473:pathology; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017469:Receptors, Fibronectin / Q000032:analysis / Q000378:metabolism*; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*
chemical_list: D016326:Extracellular Matrix Proteins; D019012:Integrin beta1; D017469:Receptors, Fibronectin