Genotoxicity of ribo- and deoxyribonucleosides of 8-hydroxyguanine, 5-hydroxycytosine, and 2-hydroxyadenine: induction of SCE in human lymphocytes and mutagenicity in Salmonella typhimurium TA 100.
The induction of SCE by ribo- and deoxyribonucleosides of 8-hydroxyguanine, 5-hydroxycytosine, and 2-hydroxyadenine was tested using human peripheral blood lymphocytes. All of these compounds caused an increase in SCE frequency. The potency of SCE induction was as follows: 5-OH-C, 5-OH-dC > 8.OH-G > 8-OH-dG > 2-OH-A, 2-OH-dA. These results suggest that the oxidized nucleosides are incorporated into DNA with different efficiencies (or are repaired with different efficiencies) and exhibit genotoxicity in human blood cells. Ribo- and deoxyribo-derivatives of 5-OH-Cyt and 2-OH-Ade also showed mutagenic activity in Salmonella typhimurium TA 100.
Arashidani
K
K
Department of Environmental Management, School of Health Sciences, University of Occupational and Environmental Health Japan, Kitakyushu, Japan.
Iwamoto-Tanaka
N
N
Muraoka
M
M
Kasai
H
H
D003596:Cytosine / Q000031:analogs & derivatives / Q000633:toxicity; D004249:DNA Damage; D003853:Deoxyribonucleosides / Q000737:chemistry / Q000633:toxicity*; D006147:Guanine / Q000031:analogs & derivatives / Q000633:toxicity; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects / Q000648:ultrastructure; D009152:Mutagenicity Tests; D009153:Mutagens / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D017354:Point Mutation; D012263:Ribonucleosides / Q000737:chemistry / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D003853:Deoxyribonucleosides; D009153:Mutagens; D012263:Ribonucleosides; C024829:8-hydroxyguanine; D006147:Guanine; C017400:5-hydroxycytosine; D003596:Cytosine; C008183:isoguanine