title: Photoacoustic calorimetry of proteins.
abstract: We have described two examples of time-resolved photoacoustic calorimetry for the study of heme protein transient intermediates. Before photoacoustic calorimetry, determining thermodynamic information on short-lived intermediates was difficult. Along with being sensitive to enthalpic and volume changes, photoacoustic calorimetry can detect conformational changes in a time-resolved manner. In complex protein systems, the interpretation of the structural origins of a conformational change is sometimes difficult. Site-directed mutagenesis has been used successfully to identify the residues that play important roles in the ligand binding to both Mb and cytochrome P450cam. In both systems the hydration state of salt bridges gave rise to volume changes that were identified through mutagenesis of the residues involved. With its increasing popularity and the power of site-directed mutagenesis, time-resolved photoacoustic calorimetry is fast becoming a technique to probe conformational dynamics in proteins.
authors.affiliation: Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana 61801, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: McLean
authors.forename: C
authors.initials: C
authors.lastname: Di Primo
authors.forename: E
authors.initials: E
authors.lastname: Deprez
authors.forename: G H
authors.initials: GH
authors.lastname: Hoa
authors.forename: S G
authors.initials: SG
authors.lastname: Sligar
mesh_terms: D000162:Acoustics / Q000295:instrumentation; D002151:Calorimetry / Q000295:instrumentation / Q000379:methods*; D019475:Camphor 5-Monooxygenase / Q000737:chemistry / Q000528:radiation effects; D006420:Hemeproteins / Q000737:chemistry* / Q000528:radiation effects; D007834:Lasers; D008956:Models, Chemical; D009211:Myoglobin / Q000737:chemistry / Q000528:radiation effects; D010782:Photolysis; D013816:Thermodynamics; D014159:Transducers
chemical_list: D006420:Hemeproteins; D009211:Myoglobin; D019475:Camphor 5-Monooxygenase