Ultraviolet-A-dependent inhibition of cytoplasmic aconitase activity of iron regulatory protein-1 in NCTC 2544 keratinocytes.
The aconitase activity of the cytoplasmic iron regulatory protein-1 of NCTC 2544 keratinocytes is effectively inhibited by physiological doses of UVA. The time course of the photoinactivation is biphasic. A fast step is first observed corresponding to about 50% inactivation after exposure to 5 J/cm2 of UVA followed by a much slower photoinactivation at higher doses. The water-soluble antioxidant N-acetylcysteine only partially inhibits the photoinduced inactivation of the cytoplasmic aconitase function, whereas the lipophilic vitamin E, the iron chelator, desferrioxamine and the superoxide dismutase inhibitor, diethyldithiocarbamate do not protect at all. As a consequence, reactive oxygen species such as O2-., H2O2 and lipid peroxides and hydroperoxides seem to play a rather minor role in the inactivation induced by the UVA photooxidative stress although an oxidative stress produced by O2-. and H2O2 is known to inhibit reversibly and effectively cytoplasmic aconitase activity in mammalian cells.
Giordani
A
A
Laboratoire de Photobiologie, INSERM U.312, Muséum National d'Histoire Naturelle, Paris, France.
Morlière
P
P
Djavaheri-Mergny
M
M
Santus
R
R
D000154:Aconitate Hydratase / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D000818:Animals; D002460:Cell Line; D006801:Humans; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D015603:Keratinocytes / Q000378:metabolism*; D008322:Mammals; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D012867:Skin; D014466:Ultraviolet Rays
D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins; D016601:RNA-Binding Proteins; D000154:Aconitate Hydratase