Influence of the phosphorylation state on the biological activity of a low-molecular mitogen from group A streptococci.
A low molecular weight mitogen (LMP) from Streptococcus pyogenes strain NY 5 was successively purified by adsorption on phenylsepharose, chromatography on Resource S and Superdex G 30 and finally by affinity chromatography on antiphosphothreonine agarose. The N-terminal protein sequence of the mitogen was determined. The occurrence of phosphoamino acids was investigated by immunoassay using monoclonal antibodies. The LMP is a threonine-phosphorylated protein different of HPR protein of PTS-system, its mitogenic activity was lost after treatment with streptococcal protein phosphatase or alkaline phosphatase. The inactivated LMP was activated by phosphorylation with phosphokinase and ATP. The active LMP was also inactivated in streptococcal cultures secreting acid protein phosphatase during the phase of phosphate limitation.
Ozegowski
J H
JH
Institut für Experimentelle Mikrobiologie, Friedrich-Schiller-Universität Jena (FSU), Germany.
Günther
E
E
Vettermann
S
S
Müller
P J
PJ
Wollweber
L
L
D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D008934:Mitogens / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology*; D008970:Molecular Weight; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010766:Phosphorylation; D010769:Phosphothreonine / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity
D000911:Antibodies, Monoclonal; D008934:Mitogens; D010769:Phosphothreonine; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D000469:Alkaline Phosphatase; D010749:Phosphoprotein Phosphatases