Sequential G-banding and fluorescent in situ hybridization on peripheral blood, bone marrow, and amniotic fluid samples.
We describe a method for fluorescent in situ hybridization (FISH) on previously G-banded slides and mounted coverslip preparations. The technique is successful on previously G-banded slides from peripheral blood and bone marrow specimens and on amniotic fluid samples from in situ harvests. Satellite sequence probes, whole chromosome paints, and unique sequence probes have all produced reliable results.
Wiktor
A
A
Department of Medical Genetics, Henry Ford Health System, Detroit, Michigan, USA.
Puskorius
R
R
Zubrickas
K
K
Van Dyke
D L
DL
D000653:Amniotic Fluid; D001853:Bone Marrow; D002871:Chromosome Banding / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007963:Leukocytes, Mononuclear