title: Characterization and reclassification of an aromatic- and chloroaromatic-degrading Pseudomonas sp., strain HV3, as Sphingomonas sp. HV3.
abstract: Phylogenetic analyses of 16S rRNA gene sequences showed that the Gram-negative aromatic- and chloroaromatic-degrading Pseudomonas sp. strain HV3 carrying the mega-plasmid pSKY4 belongs to the genus Sphingomonas. The 16SrRNA sequence is most related to Sphingomonas chlorophenolica strains ATCC 33790(T) (98.5%) and SR3 (98.4%) and Sphingomonas sp. SS86 (98.4%). The G+C content was 64 mol%, and the DNA-DNA hybridization-based relative homology of strain HV3 to the S. chlorophenolica ATCC 33790(T) and S. chlorophenolica RA2 was 59.6% and 35.9%, respectively. The results showed that although strain HV3 is related to S. chlorophenolica it differs in certain characteristics. It is therefore proposed to reclassify Pseudomonas sp. strain HV3 as Sphingomonas sp. HV3.
authors.affiliation: Department of Biosciences, University of Helsinki, Finland. kim.yrjala@helsinki.fi
authors.forename: K
authors.initials: K
authors.lastname: Yrjälä
authors.forename: S
authors.initials: S
authors.lastname: Suomalainen
authors.forename: E L
authors.initials: EL
authors.lastname: Suhonen
authors.forename: S
authors.initials: S
authors.lastname: Kilpi
authors.forename: L
authors.initials: L
authors.lastname: Paulin
authors.forename: M
authors.initials: M
authors.lastname: Romantschuk
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D011549:Pseudomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D013107:Sphingolipids / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D013107:Sphingolipids