title: Macrophage inhibition of lymphocyte and tumor cell growth is mediated by 25-hydroxycholesterol in the cell membrane.
abstract: We have previously reported that a lipid molecule in the membrane fraction of cloned macrophage hybridomas inhibited the growth of lymphocytes and several tumor cell lines. In this study, the inhibitory lipid molecule in the membrane fraction of macrophages was analyzed by thin-layer chromatography and identified as 25-hydroxycholesterol, a family of oxysterols. This conclusion was confirmed by analysis using gas chromatography-mass spectrometry. In addition, both 25-hydroxycholesterol and the lipid molecule recovered from macrophage cell membrane induced apoptosis of the murine T cell lymphoma, BW-5147. These results suggest that an oxysterol expressed in the macrophage cell membrane may participate in the regulation of cell growth through cell contact.
authors.affiliation: Department of Safety Research on Biologics, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kato
authors.forename: A
authors.initials: A
authors.lastname: Horino
authors.forename: M
authors.initials: M
authors.lastname: Taneichi
authors.forename: N
authors.initials: N
authors.lastname: Fukuchi
authors.forename: Y
authors.initials: Y
authors.lastname: Eto
authors.forename: H
authors.initials: H
authors.lastname: Ushijima
authors.forename: K
authors.initials: K
authors.lastname: Komuro
authors.forename: T
authors.initials: T
authors.lastname: Uchida
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D002455:Cell Division / Q000276:immunology; D002462:Cell Membrane / Q000737:chemistry / Q000276:immunology / Q000502:physiology; D006825:Hybridomas; D006888:Hydroxycholesterols / Q000737:chemistry / Q000494:pharmacology*; D007941:Leukemia P388 / Q000473:pathology*; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013945:Thymoma / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D006888:Hydroxycholesterols; C007997:25-hydroxycholesterol