title: Isolation of human intestinal defensins from ileal neobladder urine.
abstract: We describe the isolation of naturally occurring human intestinal defensins HD-5 and HD-6 from ileal neobladder urine and ileal mucosa. Using an antibody-based detection assay, we found multiple N-terminally processed forms of HD-5. The predominant HD-5 forms in tissue were longer than those in neobladder urine (amino acid (aa) 23-94 and 29-94 versus aa 36-94, 56-94 and 63-94) suggesting that Paneth cells store prodefensin that is processed to mature defensin during or after degranulation. Search for mature HD-6 yielded aa 69-100 as the predominant form in both sources. The ileal neobladder is a promising model to study human Paneth cell secretion.
authors.affiliation: Department of Medicine, University of California, Los Angeles 90095, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Porter
authors.forename: M A
authors.initials: MA
authors.lastname: Poles
authors.forename: J S
authors.initials: JS
authors.lastname: Lee
authors.forename: J
authors.initials: J
authors.lastname: Naitoh
authors.forename: C L
authors.initials: CL
authors.lastname: Bevins
authors.forename: T
authors.initials: T
authors.lastname: Ganz
mesh_terms: D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000737:chemistry / Q000652:urine*; D023082:Defensins; D006801:Humans; D007082:Ileum / Q000378:metabolism* / Q000637:transplantation; D008969:Molecular Sequence Data; D019879:Paneth Cells / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D016476:Urinary Reservoirs, Continent
chemical_list: D001798:Blood Proteins; D023082:Defensins; D010741:Phospholipases A