Changes in physical state of ovarian membranes during pseudopregnancy in the rat.
The role of the physical state of ovarian membranes was studied in immature rats after PMSG- and hCG-induced pseudopregnancy. Parallel changes in LH/hCG receptors, progesterone secretion and rigidity of membrane lipids were observed during pseudopregnancy. Possible structure-functional properties of the LH/hCG receptor were analyzed by the thermal perturbation technique. Thermal stability of the receptor was higher 5 days after hCG ovulatory injection to rats compared to days 11 or 18 and to control rats. Pseudopregnancy modified the quenching of protein fluorescence. The Stern-Volmer constants for controls and for rats on days 5, 11 and 18 of pseudopregnancy were found to be 2.4 and 4.6, 5.1 and 4.4, respectively, indicating that accessibility of fluorophores for the quencher was increased.
Kolena
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Scsuková
S
S
Vranová
J
J
Jezová
M
M
Tatara
M
M
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000502:physiology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005454:Fluorescence Polarization; D006064:Gonadotropins, Equine / Q000494:pharmacology; D010053:Ovary / Q000502:physiology*; D011374:Progesterone / Q000097:blood; D011555:Pseudopregnancy / Q000139:chemically induced / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D011974:Receptors, LH / Q000502:physiology; D013329:Structure-Activity Relationship
D006063:Chorionic Gonadotropin; D006064:Gonadotropins, Equine; D011974:Receptors, LH; D011374:Progesterone