title: Leukocyte-endothelial adherence correlates with pancreatic nitric oxide production in early cerulein-induced pancreatitis in rats.
abstract: The role of nitric oxide (NO) in microcirculation during the development of acute pancreatitis was not clear. An in vivo microscopic technique was used for evaluating leukocyte-endothelial adherence in the pancreatic microcirculation after induction (cerulein) of acute pancreatitis. Microdialysis was performed to detect pancreatic nitrate concentration (NO level) by high-performance liquid chromatography. Cerulein caused significantly reduced flow velocity in 1 h (31 %) and increased the number of sticking leukocytes in 2 h; both persisted for at least 3 h. Pancreatic NO level was found to be significantly elevated (2.5-fold) in 1 h and also persisted for 3 h. Both microcirculatory changes and NO elevation were significantly alleviated in cerulein-induced animals pretreated with NO synthase inhibitor (NG-nitro-L-arginine), indicating that elevation of NO could precede and account for a major portion of the observed microcirculatory changes. Furthermore, there was a strong positive correlation between numbers of adherent leukocytes and pancreatic NO level, suggesting that during the development of acute pancreatitis, NO could play an adverse role in microcirculation.
authors.affiliation: Department of Surgery, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taipei, Taiwan.
authors.forename: H M
authors.initials: HM
authors.lastname: Chen
authors.forename: M H
authors.initials: MH
authors.lastname: Shyr
authors.forename: Y T
authors.initials: YT
authors.lastname: Lau
authors.forename: T L
authors.initials: TL
authors.lastname: Hwang
authors.forename: M F
authors.initials: MF
authors.lastname: Chen
mesh_terms: D000818:Animals; D002108:Ceruletide / Q000633:toxicity*; D004730:Endothelium, Vascular / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006002:Glycodeoxycholic Acid / Q000494:pharmacology; D007962:Leukocytes / Q000473:pathology; D008297:Male; D008833:Microcirculation; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009566:Nitrates / Q000032:analysis / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism*; D010179:Pancreas / Q000098:blood supply; D010195:Pancreatitis / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004791:Enzyme Inhibitors; D009566:Nitrates; D009569:Nitric Oxide; D006002:Glycodeoxycholic Acid; D002108:Ceruletide; D019331:NG-Nitroarginine Methyl Ester