PACSIN, a brain protein that is upregulated upon differentiation into neuronal cells.
To identify genes that are differentially expressed during self-repair processes in mouse brain, we screened a subtracted cDNA library enriched for brain-specific clones. One of these clones, H74, detected a 4.4-kb mRNA predominantly expressed in brain and dorsal root ganglia neurons. Expression increased continuously during the lifespan and the state of differentiation, but decreased after entorhinal-cortex lesion. A full-length cDNA clone was isolated from a cerebellum cDNA library and characterized. Sequence analysis and database search revealed high sequence similarity to FAP52, a protein expressed in focal-adhesion contacts, and uncharacterized Echinococcus and Caenorhabditis elegans gene products. Furthermore, peptide sequences derived from human cDNA fragments showed up to 65% sequence identity at the amino acid level. The presence of a C-terminal src homology 3 (SH3) domain and its phosphorylation by casein kinase 2 (CK2) and protein kinase C (PKC) imply a role in signaling. Here we demonstrate that the gene encodes a phosphoprotein, referred to as PACSIN, with a restricted spatial and temporal expression pattern.
Plomann
M
M
Institut für Biochemie II, Med. Fakultät, Universität zu Köln, Germany. markus.plomann@uni-koeln.de
Lange
R
R
Vopper
G
G
Cremer
H
H
Heinlein
U A
UA
Scheff
S
S
Baldwin
S A
SA
Leitges
M
M
Cramer
M
M
Paulsson
M
M
Barthels
D
D
D048868:Adaptor Proteins, Signal Transducing; D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D047390:Casein Kinase II; D015815:Cell Adhesion Molecules; D002454:Cell Differentiation; D018076:DNA, Complementary / Q000235:genetics; D018728:Entorhinal Cortex / Q000166:cytology*; D005727:Ganglia, Spinal; D015723:Gene Library; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009416:Nerve Regeneration / Q000235:genetics*; D009474:Neurons / Q000166:cytology*; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics*; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D013379:Substrate Specificity; D014018:Tissue Distribution; D015854:Up-Regulation; D018909:src Homology Domains
D048868:Adaptor Proteins, Signal Transducing; D015815:Cell Adhesion Molecules; D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; C114726:Pacsin1 protein, mouse; D010750:Phosphoproteins; C108082:focal adhesion protein, 52-kDa; D047390:Casein Kinase II; D017346:Protein Serine-Threonine Kinases; D011493:Protein Kinase C