title: Influence of human serum on pharmacodynamic properties of an investigational glycopeptide, LY333328, and comparator agents against Staphylococcus aureus.
abstract: The MICs and MBCs of 15 antibiotics for two strains of Staphylococcus aureus were determined in Mueller-Hinton broth (MHB) and 90% serum-10% MHB. Subsequent experiments established that highly protein-bound antibiotics (>/=80%), such as LY333328, demonstrated higher MICs and MBCs, less killing over an 8-h interval, and shorter postantibiotic effects in 90% serum-10% MHB than in MHB alone. Albumin was demonstrated to be almost solely responsible for changes in the aforementioned pharmacodynamic parameters of LY333328.
authors.affiliation: Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada. ggzhanel@pcs.mb.ca
authors.forename: G G
authors.initials: GG
authors.lastname: Zhanel
authors.forename: I D
authors.initials: ID
authors.lastname: Kirkpatrick
authors.forename: D J
authors.initials: DJ
authors.lastname: Hoban
authors.forename: A M
authors.initials: AM
authors.lastname: Kabani
authors.forename: J A
authors.initials: JA
authors.lastname: Karlowsky
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism*; D006020:Glycopeptides; D006801:Humans; D000077427:Lipoglycopeptides; D008826:Microbial Sensitivity Tests; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000187:drug effects*; D014640:Vancomycin / Q000031:analogs & derivatives
chemical_list: D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D006020:Glycopeptides; D000077427:Lipoglycopeptides; C100708:oritavancin; D014640:Vancomycin