Modification of organ protein synthesis after surgical stress by low energy diets with different lipid/glucose ratios.
The aim of this work was to study the effects of low energy parenteral diets with different lipid/glucose ratios on rat liver and jejunal mucosa protein synthesis. The studied diets were: L0 (100% glucose, control diet), L25 (25% lipids: 75% glucose), L50 (50% lipids: 50% glucose) and L75 (75% lipids: 25 % glucose). All diets were isoenergetic and isonitrogenated, with a standard amino acid content. The diets were assayed in 93 rats with open femoral fracture immobilized by Kirschner pin insertion. The diets were administered for 4 days. On the fifth day, liver and jejunal mucosa protein synthesis were determined. Highest liver protein synthesis rates were obtained with the diet compositions: lipid/carbohydrate ratio: 25% lipids and 75% carbohydrates (expressed as energy ratio). A higher proportion of lipids significantly decreases liver protein synthesis (p <0.05). Jejunal mucosa protein synthesis followed the same pattern, with the same statistical differences.
López-Hellin
J
J
Centre for Research in Biochemistry and Molecular Biology, Hospitals Vall d'Hebron, Barcelona, Spain.
Garcia-Arumi
E
E
Farriol
M
M
Arbos
M A
MA
Andreu
A L
AL
Schwartz
S
S
D000818:Animals; D002149:Energy Intake; D005264:Femoral Fractures / Q000601:surgery; D005947:Glucose / Q000008:administration & dosage*; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000378:metabolism; D007651:Keto Acids / Q000032:analysis; D008055:Lipids / Q000008:administration & dosage*; D008099:Liver / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000008:administration & dosage; D010288:Parenteral Nutrition; D011182:Postoperative Care; D014176:Protein Biosynthesis; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D013312:Stress, Physiological
D007651:Keto Acids; D008055:Lipids; C013082:alpha-ketoisocaproic acid; D005947:Glucose; D009584:Nitrogen