Determination of montelukast sodium in human plasma by column-switching high-performance liquid chromatography with fluorescence detection.
MK-0476 (montelukast sodium) is a potent and selective cysteinyl leukotriene receptor antagonist that is being investigated in the treatment of asthma. A simple and sensitive method for the determination of MK-0476 in human plasma was developed using column-switching high-performance liquid chromatography (HPLC) with fluorescence detection. A plasma sample was injected directly onto the HPLC system consisting of a pre-column (Capcell pak MF) and an analytical column (Capcell pak C18) which were connected with a six-port switching valve. The column eluate was monitored with a fluorescence detector (excitation at 350 nm; emission at 400 nm). The calibration curve was linear in a concentration range of 1-500 ng ml(-1) for MK-0476 in human plasma. The intra-day coefficients of variation of all concentrations within the range was less than 9.2%, and the intra-day accuracy values were between 97.2 and 114.6%. This method was used to measure the plasma concentration of MK-0476 following oral administration of the drug in humans.
Ochiai
H
H
Drug Metabolism, Development Research Laboratories, Banyu Pharmaceutical Co., Ltd., Saitama, Japan.
Uchiyama
N
N
Takano
T
T
Hara
K
K
Kamei
T
T
D000085:Acetates / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D000284:Administration, Oral; D018927:Anti-Asthmatic Agents / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D003521:Cyclopropanes; D006801:Humans; D020024:Leukotriene Antagonists / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D008297:Male; D011804:Quinolines / Q000097:blood* / Q000737:chemistry / Q000494:pharmacology / Q000528:radiation effects; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D013440:Sulfides; D014466:Ultraviolet Rays
D000085:Acetates; D018927:Anti-Asthmatic Agents; D003521:Cyclopropanes; D020024:Leukotriene Antagonists; D011804:Quinolines; D013440:Sulfides; C093875:montelukast