title: Effects of palatability-induced hyperphagia and food restriction on mRNA levels of neuropeptide-Y in the arcuate nucleus.
abstract: This study examined the effect of feeding either a bland cornstarch-based diet (BCD) or a highly palatable, high fat diet containing sucrose (HPD) on hypothalamic arcuate nucleus (ARC) gene expression for neuropeptide-Y (NPY). Male Sprague-Dawley rats received either BCD ad libitum, HPD ad libitum, HPD pair-fed to the caloric intake of the BCD, or the HPD at 60% of ad libitum HPD intake for 7 days. Animals receiving the HPD ad libitum consumed more calories and gained more weight than animals receiving the BCD (P<0.001). The HPD did not affect ARC NPY mRNA levels, whether the subjects were allowed to overeat or pair-fed to the BCD (P>0.05). However, feeding the HPD at 60% of ad libitum intake of the HPD, increased NPY mRNA levels in the ARC relative to the other treatments (P<0.01). The present data are consistent with the view that NPY in ARC responds to energy deficits rather than to hyperphagia stimuli related to palatability.
authors.affiliation: Minnesota Obesity Center and Research Service (151), VA Medical Center, 1 Veterans Drive, Minneapolis, MN 55417, USA.
authors.forename: E M
authors.initials: EM
authors.lastname: Kim
authors.forename: C C
authors.initials: CC
authors.lastname: Welch
authors.forename: M K
authors.initials: MK
authors.lastname: Grace
authors.forename: C J
authors.initials: CJ
authors.lastname: Billington
authors.forename: A S
authors.initials: AS
authors.lastname: Levine
mesh_terms: D000818:Animals; D001111:Arcuate Nucleus of Hypothalamus / Q000502:physiology*; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D004041:Dietary Fats / Q000008:administration & dosage; D019422:Dietary Sucrose / Q000008:administration & dosage; D005508:Food Deprivation / Q000502:physiology*; D006963:Hyperphagia / Q000209:etiology*; D008297:Male; D009478:Neuropeptide Y / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013213:Starch / Q000008:administration & dosage; D013395:Sucrose / Q000008:administration & dosage; D013649:Taste / Q000502:physiology
chemical_list: D004040:Dietary Carbohydrates; D004041:Dietary Fats; D019422:Dietary Sucrose; D009478:Neuropeptide Y; D012333:RNA, Messenger; D013395:Sucrose; D013213:Starch