Steady-state properties of sodium channels from healthy and tumorous human brain.
This extensive bilayer study of unpurified human brain channels from non-diseased and tumorous human brain involves more than 300 lipid bilayer experiments. Single channel conductances and subconductances, single channel fractional open times, the voltage-dependence of tetrodotoxin (TTX) block and the steady-state activation behavior of four different human brain synaptosomal preparations have been examined. Reproducible values have been obtained for the molecular electrophysiological parameters and their standard deviations, providing a database for future comparisons involving disease or drug-related changes in molecular sodium channel functions. In comparison with sodium channels from other species and under other experimental conditions, the bilayer system proved to be a reliable experimental setting. Despite the very different histology of the tissue probes, there were no significant differences in any of the examined electrophysiological features.
Frenkel
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C
Klinik und Poliklinik für Anästhesiologie und spezielle Intensivmedizin, Universität Bonn, Sigmund-Freud-Str. 25, D-53105, Bonn, Germany.
Wartenberg
H C
HC
Duch
D S
DS
Urban
B W
BW
D001921:Brain / Q000378:metabolism* / Q000648:ultrastructure; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D003627:Data Interpretation, Statistical; D004553:Electric Conductivity; D006801:Humans; D015640:Ion Channel Gating; D008051:Lipid Bilayers; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D015203:Reproducibility of Results; D026941:Sodium Channel Blockers; D015222:Sodium Channels / Q000502:physiology*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology
D008051:Lipid Bilayers; D026941:Sodium Channel Blockers; D015222:Sodium Channels; D013779:Tetrodotoxin