Recent developments of magnetic beads for use in nucleic acid purification.
The performance of Magarose, an agarose-based bead containing a paramagnetic component has been evaluated. The anion exchanger DEAE-Magarose is effective at binding DNA from a crude cell lysate. The plasmid pBluescript was isolated from 1.5 ml Escherichia coli JM109 cell culture, following alkaline lysis yielding 8.2 micrograms high-quality DNA. Under similar binding conditions 21 micrograms of salmon sperm DNA bound to the ion exchangers. The affinity medium oligo-dT Magarose was demonstrated to bind 75 mumol of an oligo-dA probe/g of medium by hybridization. Under similar conditions mRNA could be isolated from a preparation of baby hamster cell total RNA. The magnetic susceptibility of Magarose is very high, facilitating the use of this separation technique for rapid batch chromatographic processes.
Levison
P R
PR
Whatman International Ltd., Maidstone, Kent, UK.
Badger
S E
SE
Dennis
J
J
Hathi
P
P
Davies
M J
MJ
Bruce
I J
IJ
Schimkat
D
D
D000818:Animals; D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange; D006224:Cricetinae; D004269:DNA, Bacterial / Q000302:isolation & purification; D004926:Escherichia coli / Q000737:chemistry; D008280:Magnetics; D008297:Male; D009696:Nucleic Acids / Q000302:isolation & purification*; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification; D010957:Plasmids / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification; D012474:Salmon; D012685:Sepharose; D013094:Spermatozoa / Q000737:chemistry
D000837:Anion Exchange Resins; D004269:DNA, Bacterial; D009696:Nucleic Acids; D009841:Oligonucleotides; D012333:RNA, Messenger; D012685:Sepharose