DNA damage evaluated by the alkaline comet assay in lymphocytes of humans anaesthetized with isoflurane.
In the present paper, we report data on the possible DNA damage, induced in vivo by isoflurane using the alkaline single cell gel electrophoresis technique (SCGE-comet assay) in patients before/after anaesthesia and in control group. Twelve patients, aged 22-66 years old, were anaesthetized for elective abdominal surgery with isoflurane in oxygen for 120-162 min (mean: 133.2 min). Venous blood samples were obtained from the patients before the induction of anaesthesia, at 60 and 120 min of anaesthesia and on the first, third and fifth following days of anaesthesia. SCGE was examined in 100 cells from each specimen graded as undamaged, intermediate and tailed nuclei. The number of undamaged nucleus was almost same in control and in patients before anaesthesia. However, significant differences were observed in proportion of undamaged, intermediate and tailed nucleus of patients at 60 and 120 min of anaesthesia and on the first day. DNA damage started to return to normal rates after the third day of anaesthesia and were almost identical with the rates of control group five days later.
Sardaş
S
S
Department of Toxicology, Gazi University, Faculty of Pharmacy, 06330, Ankara, Turkey.
Karabiyik
L
L
Aygün
N
N
Karakaya
A E
AE
D000328:Adult; D000368:Aged; D018685:Anesthetics, Inhalation / Q000009:adverse effects*; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D004996:Ethidium; D005069:Evaluation Studies as Topic; D005456:Fluorescent Dyes; D006801:Humans; D007530:Isoflurane / Q000009:adverse effects*; D008214:Lymphocytes / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D008875:Middle Aged; D009152:Mutagenicity Tests; D012680:Sensitivity and Specificity
D018685:Anesthetics, Inhalation; D005456:Fluorescent Dyes; D007530:Isoflurane; D004996:Ethidium