The effect of Epstein-Barr virus gene BHRF1 expression on radioresistance of nasopharyngeal carcinoma cells.
In order to investigate the effect of the expression of Epstein-Barr virus gene BHRF1 on the apoptotic resistance of nasopharyngeal carcinoma cells to radiation, a highly expressing vector for BHRF1 was constructed and transfected into the nasopharyngeal carcinoma cell line CNE2. Then, the biologic alterations of the cells were tested after 60Co radiation. The results showed that, in the BHRF1-expressing cells, the apoptotic index was far lower than in the control groups after 60Co radiation, and cells recovered faster from the radiation, with a higher cell-proliferative rate, stronger ability of colony formation and tumor development in nude mice than that in the control groups. Given the functional homology of BHRF1 with bcl-2, our data indicate that BHRF1 expression could prohibit nasopharyngeal carcinoma cellular apoptosis caused by radiation and in this way contribute to oncogenic transformation.
Huang
H
H
Department of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai, PR of China. huanghai@ecmu.org.cn
Pan
X H
XH
Zhou
J H
JH
Yu
L
L
Kong
X T
XT
Zhou
S M
SM
Li
Z J
ZJ
Fu
Q
Q
Sun
X Y
XY
D000818:Animals; D017209:Apoptosis / Q000528:radiation effects*; D015152:Blotting, Northern; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D016009:Chi-Square Distribution; D004587:Electrophoresis, Agar Gel; D015972:Gene Expression Regulation, Neoplastic; D005814:Genes, Viral; D005822:Genetic Vectors; D004854:Herpesvirus 4, Human / Q000235:genetics*; D020287:In Situ Nick-End Labeling; D051379:Mice; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000532:radiotherapy / Q000821:virology*; D014162:Transfection / Q000379:methods; D014407:Tumor Cells, Cultured / Q000528:radiation effects