Apoptosis induced by modified ribonucleosides in human cell culture systems.
The in vitro modulation of apoptosis and cell proliferation by modified in comparison with non-modified ribonucleosides was investigated for the first time using peripheral blood lymphocytes, HL-60 cells and Caco-2 cells as human cell culture models. Modulating effects of several ribonucleosides were found in the range of 10(-7)-10(-3) mol/l. The following ribonucleosides induced significant apoptosis of HL-60 cells: adenosine, N6-dimethyladenosine, N6-(2-isopentenyl)-adenosine, N2-dimethylguanosine. A significant apoptotic effect on PBL was found with N6-dimethyladenosine and N6-(2-isopentenyl)-adenosine. N6-Dimethyladenosine, N6-(2-isopentenyl)-adenosine and guanosine had a pronounced inhibitory effect on Caco-2 cell apoptosis. Regarding the known function of ribonucleosides as pathobiochemical marker molecules for cancer, the possibility of a selective apoptotic effect against malignant cells is discussed.
Meisel
H
H
Bundesanstalt für Milchforschung, Institut für Chemie und Physik, Kiel, Germany. meisel@bafm.de
Günther
S
S
Martin
D
D
Schlimme
E
E
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003110:Colonic Neoplasms; D006151:Guanosine / Q000031:analogs & derivatives / Q000494:pharmacology; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007288:Inosine / Q000031:analogs & derivatives / Q000494:pharmacology; D007541:Isopentenyladenosine / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D012263:Ribonucleosides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D012263:Ribonucleosides; D006151:Guanosine; D007288:Inosine; D007541:Isopentenyladenosine; D000241:Adenosine