title: Antibiotic stress induces a large amount of outer membrane protein in Pseudomonas aeruginosa.
abstract: To investigate the bacterial response to antibiotic stress, we analyzed the outer membrane proteins of Pseudomonas aeruginosa grown in the presence of a sub-minimum inhibitory concentration of antibiotics. Among the antibiotics tested, fluoroquinolones and streptonigrin induced a large amount of outer membrane protein with a molecular mass of 43 kDa. This protein is most likely the stress-responsive protein, since the quinolone-resistant mutants with a higher minimum inhibitory concentration of antibiotic than the wild-type strain produced a large amount of 43-kDa protein only in the presence of sub-minimum inhibitory concentration of the mutants itself, but not that of the antibiotic-susceptible wild-type strain. The sequence of N-terminal 15 amino acids of the 43-kDa protein was identical to that of pyocin R1. However, purified pyocin R1 failed to accumulate in the outer membrane. Thus, we concluded that the 43-kDa protein (pyocin R1) is the antibiotic-stress-induced outer membrane protein.
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nakajima
authors.forename: M
authors.initials: M
authors.lastname: Hoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Nakae
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D024841:Fluoroquinolones; D006801:Humans; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D011709:Pyocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D013308:Streptonigrin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D001425:Bacterial Outer Membrane Proteins; D024841:Fluoroquinolones; D011709:Pyocins; D013308:Streptonigrin