[Correlation between pyrimidine nucleoside phosphorylase (PyNPase)/platelet-derived endothelial cell growth factor and histological prognostic factor, and influences of 5'-deoxy-5-fluorouridine (5'-DFUR) administration on PyNPase levels. 5'-DFUR Joint Research Group in the Osaka Area for Gastric Cancer].
OBJECTIVE
Pyrimidine nucleoside phosphorylase (PyNPase), among which thymidine phosphorylase (dThdPase) exists mainly in human tumor tissues, is an enzyme to convert 5'-deoxy-5-fluorouridine (5'-DFUR) to 5-fluorouracil. Recently, it was reported that dThdPase was identical to platelet-derived endothelial cell growth factor, angiogenetic factor. Therefore, we expect that there is possibility of dThdPase being a prognostic factor.


METHODS
We investigated for a possible correlation between PyNPase activities in tumor tissues and prognostic factors of histological findings, examined the influences of preoperative oral 5'-DFUR administration to PyNPase levels and investigated for a correlation between HPLC methods and ELISA methods in patients with gastric cancer.


RESULTS
Higher levels of PyNPase were observed in patients with advanced t,n,v, and ly factors. PyNPase levels decreased by 5'-DFUR in patients with differentiated cases. A high correlation was found between HPLC and ELISA methods.


CONCLUSIONS
This study suggests that we must investigate possibility of PyNPase being a prognostic factor in more detail.
Yamasaki
Y
Y
Dept. of Surgery, Osaka Kousei Nenkin Hospital.
Kuwata
K
K
Hioki
K
K
Sowa
M
M
Hirakawa
K
K
Okajima
K
K
Isozaki
H
H
Iwanaga
T
T
Furukawa
H
H
Nakano
H
H
Watanabe
A
A
Takami
M
M
Tsukahara
Y
Y
Matsunaga
S
S
Kikkawa
N
N
Gotoh
T
T
Tokuda
H
H
Takahashi
S
S
Toyohara
M
M
Yagi
M
M
Takebayashi
J
J
Nakajima
T
T
Miyazaki
H
H
Kuwata
H
H
Nakazato
H
H
D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005467:Floxuridine / Q000008:administration & dosage*; D006801:Humans; D010430:Pentosyltransferases / Q000378:metabolism*; D011379:Prognosis; D057845:Pyrimidine Phosphorylases; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology*; D013939:Thymidine Phosphorylase / Q000378:metabolism
D000970:Antineoplastic Agents; D005467:Floxuridine; D010430:Pentosyltransferases; D057845:Pyrimidine Phosphorylases; D013939:Thymidine Phosphorylase; C025034:doxifluridine