title: Cloning of the alphaA-crystallin genes of a blind cave form and the epigean form of Astyanax fasciatus: a comparative analysis of structure, expression and evolutionary conservation.
abstract: In the present study we have analyzed the integrity and expression of the alphaA-crystallin gene, that codes for a major structural component of the lens, in a blind cave form of the teleostean fish, Astyanax fasciatus. This is the first alphaA-crystallin gene cloned from a teleostean fish. Sequence comparison of this cave-form gene with its epigean conspecific and with homologs of distantly related taxa has illustrated conservation of regulatory and coding regions. Although no crystallin proteins are produced in the lens of the cave form, and the mRNA of this gene could not be detected by in situ hybridization of different developmental stages, the promoter region of cave-fish alphaA-crystallin is functionally intact. The deduced amino-acid sequence of the alphaA-crystallin gene of the cave form differs from that of its epigean conspecific at only one position (139). This is within an important, small heat-shock protein-related region, HCR2. A comparison of the 5'-flanking regions of the A. fasciatus alphaA-crystallin gene with the chicken homolog revealed the high conservation of lens-specific regulatory sequences and further demonstrates the evolutionary conservation of this gene. 1988 Elsevier Science B.V.
authors.affiliation: Institut für Zellbiochemie und klinische Neurobiologie, Universitätskrankenhaus Eppendorf, Martinistrasse 52, D-20246, Hamburg, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Behrens
authors.forename: H
authors.initials: H
authors.lastname: Wilkens
authors.forename: H
authors.initials: H
authors.lastname: Schmale
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001766:Blindness / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D003459:Crystallins / Q000235:genetics*; D019143:Evolution, Molecular; D005091:Exons / Q000235:genetics; D005399:Fishes / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D007908:Lens, Crystalline / Q000737:chemistry; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D003459:Crystallins; D012333:RNA, Messenger