Attractin (DPPT-L), a member of the CUB family of cell adhesion and guidance proteins, is secreted by activated human T lymphocytes and modulates immune cell interactions.
Attractin is a normal human serum glycoprotein of 175 kDa that is rapidly expressed on activated T cells and released extracellularly after 48-72 hr. We have cloned attractin and find that, as in its natural serum form, it mediates the spreading of monocytes that become the focus for the clustering of nonproliferating T lymphocytes. There are two mRNA species with hematopoietic tissue-specific expression that code for a 134-kDa protein with a putative serine protease catalytic serine, four EGF-like motifs, a CUB domain, a C type lectin domain, and a domain homologous with the ligand-binding region of the common gamma cytokine chain. Except for the latter two domains, the overall structure shares high homology with the Caenorhabditis elegans F33C8.1 protein, suggesting that attractin has evolved new domains and functions in parallel with the development of cell-mediated immunity.
Duke-Cohan
J S
JS
Division of Tumor Immunology, Dana-Farber Cancer Institute, Harvard Medical School, 44 Binney Street, Boston, MA 02115, USA.
Gu
J
J
McLaughlin
D F
DF
Xu
Y
Y
Freeman
G J
GJ
Schlossman
S F
SF
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D017173:Caenorhabditis elegans / Q000737:chemistry; D015815:Cell Adhesion Molecules / Q000737:chemistry*; D002465:Cell Movement / Q000187:drug effects; D003001:Cloning, Molecular; D016207:Cytokines / Q000737:chemistry; D004815:Epidermal Growth Factor / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D015801:Helminth Proteins / Q000737:chemistry; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry; D013601:T-Lymphocytes / Q000502:physiology*
D001798:Blood Proteins; D015815:Cell Adhesion Molecules; D016207:Cytokines; D006023:Glycoproteins; D015801:Helminth Proteins; D012333:RNA, Messenger; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D012697:Serine Endopeptidases