A comparative study on the efficacy of CD8-positive cells in enhancing allogeneic bone marrow engraftment: cell sorting vs microbead selection.
We have used a superparamagnetic microbead selection system to positively select a murine bone marrow CD8+ cell population. The functional ability of these cells to enhance allogeneic bone marrow engraftment was compared with that of fluorescence activated cell sorter purified CD8+ cells. The CD8+ cell population prepared by the microbead selection procedure was as effective as cell sorter purified CD8+ cells in enhancing T cell-depleted allogeneic bone marrow engraftment in lethally irradiated mice. Phenotypic characterization of these cells shows that most of these CD8+ cells express CD3 and the T cell antigen receptor complex.
Sen-Majumdar
A
A
Geneic Sciences Inc., Menlo Park, CA, USA.
Sheehan
K
K
Guo
G
G
Allegre
G
G
Simone
J
J
Harvey
M
M
Chow
P
P
Moen
R
R
Smith
A
A
D000818:Animals; D016026:Bone Marrow Transplantation; D018414:CD8-Positive T-Lymphocytes / Q000473:pathology*; D005434:Flow Cytometry; D006085:Graft Survival; D008212:Lymphocyte Depletion / Q000379:methods*; D051379:Mice; D008863:Microspheres; D014184:Transplantation, Homologous