title: Increased choline transport in erythrocytes from mice infected with the malaria parasite Plasmodium vinckei vinckei.
abstract: Parasitized erythrocytes from mice infected with the murine malaria parasite Plasmodium vinckei vinckei showed a marked increase in the rate of influx of choline compared with erythrocytes from uninfected mice. In contrast, uninfected erythrocytes from P. vinckei-infected animals transported choline at the same rate as those from uninfected mice. The increased influx of choline into parasitized cells was via two discrete routes. One was a saturable pathway with a Km similar to that of the choline carrier of normal erythrocytes but a Vmax approx. 20-fold higher than that observed in uninfected cells. The other was a non-saturable pathway inhibited by furosemide. At choline concentrations within the normal physiological plasma concentration range, the former pathway contributed approx. two-thirds and the latter approx. one-third of the influx of choline into parasitized cells. The characteristics of the furosemide-sensitive pathway were similar to those of a broad-specificity pathway that is induced in human erythrocytes infected in vitro with Plasmodium falciparum. The results of this study rule out the possibility that the induced transport pathway of P. falciparum-infected erythrocytes is an artifact arising in vitro from the long-term culture of parasitized cells and provide evidence that this pathway makes a significant contribution to the uptake of choline into the parasitized cells of malaria-infected animals.
authors.affiliation: Division of Biochemistry and Molecular Biology, Faculty of Science, Australian National University, Canberra ACT 0200, Australia.
authors.forename: H M
authors.initials: HM
authors.lastname: Staines
authors.forename: K
authors.initials: K
authors.lastname: Kirk
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002794:Choline / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D008288:Malaria / Q000097:blood* / Q000469:parasitology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D010961:Plasmodium / Q000254:growth & development
chemical_list: D005665:Furosemide; D002794:Choline