Molecular cloning of a lobster Gbeta subunit and Gbeta expression in olfactory receptor neuron dendrites and brain neuropil.
We have isolated from the olfactory organ of the American lobster (Homarus americanus) two cDNA clones with homology to beta subunits of G proteins. LobGbeta1 contained a complete open reading frame that predicted an amino acid sequence with >80% identity to Gbeta sequences from other species. LobGbeta2 was a fragment of an open reading frame whose predicted amino acid sequence had 65-69% identity to other Gbeta sequences. LobGbeta2 mRNA was not detectable in the brain, eye plus eyestalk, leg, dactyl, olfactory organ, or tail muscle. In contrast, lobGbeta1 was expressed in all these tissues as a single mRNA species of 6.4 kb and a protein of 37 kD. In the brain and olfactory organ, Gbeta immunoreactivity was almost exclusively confined to neurites: the neuropil regions of the brain and the outer dendrites of the olfactory receptor neurons. Coimmunoprecipitation revealed that lobster Gbeta interacted with both Galpha s and Galpha q. LobGbeta1 is likely to be involved in a wide range of signaling events including olfactory transduction and synaptic transmission in the brain.
Xu
F
F
Department of Physiology, University of Kentucky, Lexington 40536-0084, USA.
Hollins
B
B
Landers
T M
TM
McClintock
T S
TS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000166:cytology*; D001923:Brain Chemistry / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D004274:DNA, Recombinant / Q000096:biosynthesis / Q000235:genetics; D003712:Dendrites / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000096:biosynthesis*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000378:metabolism*; D019581:Neuropil / Q000166:cytology / Q000378:metabolism*; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D016366:Open Reading Frames; D012679:Sense Organs / Q000502:physiology
D004274:DNA, Recombinant; D019204:GTP-Binding Proteins