title: A new subculture and nematocidal assay using a species of diplogastridae.
abstract: A new subculture method and a novel microplate assay for nematocidal activity using a species of Diplogastridae have been developed. The assay gives results rapidly, with high sensitivity in 4 h, and indicated good correlation between action mechanism and the nematode shape when examining 15 known compounds, including the antiparasitic avermectin, antimalarial quinine, and the gamma-amino-n-butyric acidA (GABAA) activated Cl- channel antagonist picrotoxinin. Thus new assay could be used as a primary screening method for new nematocidal compounds.
authors.affiliation: School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Yanagida
authors.forename: R
authors.initials: R
authors.lastname: Matsuhashi
authors.forename: I
authors.initials: I
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Satou
authors.forename: K
authors.initials: K
authors.lastname: Koike
authors.forename: T
authors.initials: T
authors.lastname: Nikaido
authors.forename: S
authors.initials: S
authors.lastname: Akao
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D009348:Nematoda / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D000969:Antinematodal Agents; D003470:Culture Media