Uncoupling protein 3 is reduced in skeletal muscle of NIDDM patients.
Two recently described proteins in the mitochondrial uncoupling protein (UCP) family, UCP-2 and UCP-3, have been linked to phenotypes of obesity and NIDDM. We determined the mRNA levels of UCP-2 and UCP-3 in skeletal muscle of NIDDM patients and of healthy control subjects. No difference in the mRNA levels or in the protein expression of UCP-2 was observed between the two groups. In contrast, mRNA levels of UCP-3 were significantly reduced in skeletal muscle of NIDDM patients compared with control subjects. In the NIDDM patients, a positive correlation between UCP-3 expression and whole-body insulin-mediated glucose utilization rate was also noted. These results suggest that UCP-3 regulation may be altered in states of insulin resistance.
Krook
A
A
Department of Clinical Physiology, Karolinska Hospital, Stockholm, Sweden. ankr@klinfys.ks.se
Digby
J
J
O'Rahilly
S
S
Zierath
J R
JR
Wallberg-Henriksson
H
H
D001786:Blood Glucose / Q000378:metabolism; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000097:blood / Q000502:physiology; D007473:Ion Channels; D008875:Middle Aged; D008931:Mitochondria, Muscle / Q000378:metabolism; D024101:Mitochondrial Proteins; D018482:Muscle, Skeletal / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D014158:Transcription, Genetic; D000071247:Uncoupling Protein 3
D001786:Blood Glucose; D002352:Carrier Proteins; D017931:DNA Primers; D005947:Glucose; D006442:Glycated Hemoglobin; D007328:Insulin; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071247:Uncoupling Protein 3; C000604392:UCP3 protein, human