Rebamipide protects against oxygen radical-mediated gastric mucosal injury in rats.
Rebamipide, a novel antiulcer agent, has been shown to protect against gastric injury by free radicals. The effect of rebamipide was examined using two rat models of mucosal injury: the stomach was exposed to luminal perfusion of 10 mM H2O2 for 10 min or to local ischemia for 30 min. The effect of deferoxamine, a chelator of Fe3+, was also evaluated to determine whether Fe3+-mediated production of hydroxyl radicals contributed to the damage induced by H2O2. The pylorus was ligated and a double-lumen cannula was inserted into the forestomach for luminal perfusion. [51Cr]EDTA was administered intravenously and mucosal integrity was monitored by measuring blood-to-lumen [51Cr]EDTA clearance. Rebamipide reduced the increase in EDTA clearance induced by ischemia or H2O2. Furthermore, deferoxamine attenuated the H2O2-induced increase. These results suggest that rebamipide has a protective effect against oxygen radical-mediated gastric damage and that Fe3+ is involved in the H2O2-induced injury.
Kurokawa
T
T
1st Department of Internal Medicine, Nagoya City University Medical School, Nagoya, Japan.
Joh
T
T
Ikai
M
M
Seno
K
K
Yokoyama
Y
Y
Itoh
M
M
D000409:Alanine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D005609:Free Radicals; D005753:Gastric Mucosa / Q000187:drug effects*; D008297:Male; D010100:Oxygen / Q000378:metabolism*; D015363:Quinolones / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control
D000975:Antioxidants; D005609:Free Radicals; D015363:Quinolones; C052785:rebamipide; D000409:Alanine; D010100:Oxygen