title: Semiconductor nanocrystals as fluorescent biological labels.
abstract: Semiconductor nanocrystals were prepared for use as fluorescent probes in biological staining and diagnostics. Compared with conventional fluorophores, the nanocrystals have a narrow, tunable, symmetric emission spectrum and are photochemically stable. The advantages of the broad, continuous excitation spectrum were demonstrated in a dual-emission, single-excitation labeling experiment on mouse fibroblasts. These nanocrystal probes are thus complementary and in some cases may be superior to existing fluorophores.
authors.affiliation: Department of Chemistry, University of California, Berkeley, CA 94720, USA.
authors.forename: M
authors.initials: M
authors.lastname: Bruchez
authors.forename: M
authors.initials: M
authors.lastname: Moronne
authors.forename: P
authors.initials: P
authors.lastname: Gin
authors.forename: S
authors.initials: S
authors.lastname: Weiss
authors.forename: A P
authors.initials: AP
authors.lastname: Alivisatos
mesh_terms: D016475:3T3 Cells; D000199:Actins; D000818:Animals; D019921:Biotinylation; D019187:Cadmium Compounds; D002467:Cell Nucleus / Q000648:ultrastructure*; D003460:Crystallization; D003599:Cytoskeleton / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015336:Molecular Probe Techniques; D018036:Selenium Compounds; D012666:Semiconductors; D012822:Silicon Dioxide; D012995:Solubility; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods; D013440:Sulfides
chemical_list: D000199:Actins; D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D018036:Selenium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D012822:Silicon Dioxide; C058667:cadmium selenide