title: In vitro production of progesterone and estradiol by rat granulosa cells regulated by cabergoline and prolactin.
abstract: Cabergoline (CG), a dopamine agonist, and prolactin (PRL) added in vitro to isolated granulosa cells from immature rats cultured in serum-free medium inhibited FSH-stimulated production of progesterone. The lowest effective concentrations of CG and PRL were 10(-8)M and 1 ng/mL, respectively. In combination, the inhibitory activities were additive. CG at 10(-8) M also suppressed estradiol production by isolated rat granulosa cells; whereas PRL did not. In conclusion, CG inhibits FSH-stimulated progesterone and estradiol production by rat granulosa cells.
authors.affiliation: Dept. of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Negishi
authors.forename: I
authors.initials: I
authors.lastname: Furuta
authors.forename: K
authors.initials: K
authors.lastname: Ushigoe
authors.forename: S
authors.initials: S
authors.lastname: Fujimoto
authors.forename: S S
authors.initials: SS
authors.lastname: Koide
mesh_terms: D000818:Animals; D000077465:Cabergoline; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D018491:Dopamine Agonists / Q000494:pharmacology; D004873:Ergolines / Q000494:pharmacology*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism*; D011388:Prolactin / Q000494:pharmacology*; D011863:Radioimmunoassay; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016895:Culture Media, Serum-Free; D018491:Dopamine Agonists; D004873:Ergolines; D011374:Progesterone; D004958:Estradiol; D011388:Prolactin; D005640:Follicle Stimulating Hormone; D000077465:Cabergoline