[Chitinase production by a strain of Streptomyces griseoruber isolated from the rhizosphere of sugar cane].
Twenty nine mesophilic and 19 thermophilic actinomycetes from sugar cane phyllosphere, rhizosphere and root-free soil were isolated. Twelve mesophilic and 7 thermophilic strains produced clear zones of hydrolyses on colloidal chitin agar and hydrolysed a chitin colloidal suspension. The rhizosphere strains were significantly more chitinolytic (p < 0.05) than the root-free soil strains. Streptomyces griseoruber 202 produced chitinase in colloidal chitin liquid medium with an activity of 108 micrograms of colloidal chitin hydrolysed/mg protein/hour, at pH 4.6 and 40 degrees C. This strain also produced chitinase in a medium with insect exoskeleton powder.
Carrillo
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Facultad de Ciencias Agrarias, Universidad Nacional de Jujuy, Argentina. carrillo@ciunsa.edu.ar
Gómez Molina
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D000192:Actinomycetales / Q000201:enzymology / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis*; D018517:Plant Roots / Q000382:microbiology; D010945:Plants, Edible / Q000382:microbiology*; D012988:Soil Microbiology; D013045:Species Specificity; D013302:Streptomyces / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification
D001426:Bacterial Proteins; D002686:Chitin; D002688:Chitinases