Correlative microscopy using FluoroNanogold on ultrathin cryosections. Proof of principle.
We demonstrate a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to be a versatile reporter system for immunocytochemical labeling of ultrathin cryosections. FNG-labeled molecules in the same ultrathin cryosections can be resolved by two imaging techniques (i.e., fluorescence and electron microscopy). Lactoferrin, a marker protein for the specific granules in human neutrophils, was employed as the target for FNG immunolabeling. The spatial resolution of the fluorescence signal from FNG-labeled specific granules was compatible with that of silver-enhanced gold signal from the same granules in electron microscopy. Our results confirm that FNG can be used as a probe for high-resolution correlation between immunofluorescence and electron microscopy.
Takizawa
T
T
Department of Anatomy, Jichi Medical School, Tochigi, Japan.
Suzuki
K
K
Robinson
J M
JM
D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D007781:Lactoferrin / Q000032:analysis; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009504:Neutrophils / Q000737:chemistry / Q000648:ultrastructure
D007781:Lactoferrin