title: Communication between Hin recombinase and Fis regulatory subunits during coordinate activation of Hin-catalyzed site-specific DNA inversion.
abstract: The Hin DNA invertase becomes catalytically activated when assembled in an invertasome complex containing two Fis dimers bound to an enhancer segment. The region of Fis responsible for transactivation of Hin contains a mobile beta-hairpin arm that extends from each dimer subunit. We show here that whereas both Fis dimers must be capable of activating Hin, Fis heterodimers that have only one functional activating beta-arm are sufficient to form catalytically competent invertasomes. Analysis of homodimer and heterodimer mixes of different Hin mutants suggests that Fis must activate each subunit of the two Hin dimers that participate in catalysis. These experiments also indicate that all four Hin subunits must be coordinately activated prior to initiation of the first chemical step of the reaction and that the process of activation is independent of the catalytic steps of recombination. We propose a molecular model for the invertasome structure that is consistent with current information on protein-DNA structures and the topology of the DNA strands within the recombination complex. In this model, a single Fis activation arm could contact amino acids from both Hin subunits at the dimer interface to induce a conformational change that coordinately positions the active sites close to the scissile phosphodiester bonds.
authors.affiliation: Department of Biological Chemistry, School of Medicine, University of California at Los Angeles, Los Angeles, California 90095-1737, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Merickel
authors.forename: M J
authors.initials: MJ
authors.lastname: Haykinson
authors.forename: R C
authors.initials: RC
authors.lastname: Johnson
mesh_terms: D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism*; D007446:Chromosome Inversion; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000737:chemistry* / Q000378:metabolism*; D004251:DNA Transposable Elements; D019281:Dimerization; D004742:Enhancer Elements, Genetic; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D046911:Macromolecular Substances; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D017354:Point Mutation; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D011995:Recombination, Genetic; D015533:Transcriptional Activation
chemical_list: D002352:Carrier Proteins; D004251:DNA Transposable Elements; D035021:Factor For Inversion Stimulation Protein; D034982:Integration Host Factors; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D004247:DNA; D004254:DNA Nucleotidyltransferases; C059997:Hin recombinase