Comparison of amphotropic and pseudotyped VSV-G retroviral transduction in human CD34+ peripheral blood progenitor cells from adult donors with HIV-1 infection or cancer.
In this study we compared the transduction efficiency of conventional amphotropic MoMLV (LPONL[A]) with the MoMLV pseudotyped with that of VSV-G (LPONL[G]) in peripheral blood progenitor cells (PBPCs) from cancer patients and human immunodeficiency virus (HIV)-infected donors. The results showed that LPONL(A) and LPONL(G) infected the progenitor cells from these sources with equal efficiencies. The transgene neoR was detectable by polymerase chain reaction assay in colonies from 14-day colony-forming unit (CFU) assays and in those derived from long-term culture-initiating cell (LTC-ICs) assays. Although the overall levels of transduction efficiency were similar in cord blood and PBPCs from noninfected cancer donors (25-22%) when either LPONL(G) or LPONL(A) was used, they were significantly lower in HIV-1-infected donors compared with noninfected cancer donors when LPONL(G) was used (13 vs. 25%; p = 0.027), and when LPONL(A) was used (12 vs. 22%; p = 0.087). The clonogenic potentials of infected and noninfected CD34+ cells were similar; thus no toxicity could be attributed to the virus preparation. We conclude that PBPCs from HIV-1-infected individuals are transduced less efficiently than those from non-HIV-infected cancer donors. Nonetheless, PBPCs from HIV-infected persons serve as potential targets in gene therapy for acquired immune deficiency syndrome.
Yam
P Y
PY
Department of Virology and Infectious Diseases, City of Hope National Medical Center, Duarte, CA 91010, USA.
Yee
J K
JK
Ito
J I
JI
Sniecinski
I
I
Doroshow
J H
JH
Forman
S J
SJ
Zaia
J A
JA
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D018952:Antigens, CD34 / Q000097:blood; D001943:Breast Neoplasms / Q000235:genetics; D015316:Genetic Therapy; D005822:Genetic Vectors; D015497:HIV-1; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D008979:Moloney murine leukemia virus / Q000235:genetics; D009369:Neoplasms / Q000821:virology; D014161:Transduction, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000235:genetics*
D018952:Antigens, CD34