Inhibition of immediate type allergic reactions by the aqueous extract of Kum-Hwang-San.
We studied the effect of aqueous extract of Kum-Hwang-San (KHS) on mast cell-mediated immediate type allergic reactions. KHS (1-100 microg/site) inhibited concentration-dependently mast cell-dependent ear swelling response induced by compound 48/80 (200 microg/site) in mice by both topical and intradermal application. KHS (0.1-100 microg/site) inhibited concentration-dependently passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP) IgE in rats by both topical and intradermal application. KHS also inhibited concentration-dependently the histamine release from the rat peritoneal mast cells (RPMC) by compound 48/80 and anti-DNP IgE. Moreover, KHS had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-alpha (TNF-alpha) secretion from RPMC. These results indicate that KHS inhibits immediate type allergic reactions by inhibition of histamine release and TNF-alpha secretion from mast cells in vivo and in vitro.
Kim
H
H
College of Pharmacy, Wonkwang University, Iksan, Chonbuk, South Korea. hmkim@med.wonkwang.ac.kr
Shin
H
H
Lee
E
E
Lee
J
J
Jung
J
J
An
N
N
Lee
Y
Y
Kim
D
D
Jippo
T
T
Kitamura
Y
Y
D000818:Animals; D004140:Dinitrophenols / Q000276:immunology; D004342:Drug Hypersensitivity / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006636:Histamine Release / Q000187:drug effects; D006969:Hypersensitivity, Immediate / Q000188:drug therapy*; D007073:Immunoglobulin E / Q000494:pharmacology; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010529:Peritoneal Cavity / Q000166:cytology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D003189:p-Methoxy-N-methylphenethylamine
D004140:Dinitrophenols; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha; D007073:Immunoglobulin E; D003189:p-Methoxy-N-methylphenethylamine