Effect of a 17-member azalide on tumor cell growth.
The action of the 17-member azalide 4'-demycarosyl-20-deoxo-20-(di-N-benzylamino)-8a-aza-8a-homotyl osin was examined in vitro using five different human cell lines: laryngeal carcinoma (Hep2), pancreatic carcinoma (MiaPaCa2), breast carcinoma (MCF7), neuroblastoma, and normal diploid fibroblast (Hef522). After exposure, the cell growth was arrested, and morphological changes occurred in a dose-dependent manner. At a concentration of 10(-4) M the azalide completely inhibited the growth of all cell lines examined and induced morphological changes such as cell shrinkage, chromatin condensation, and DNA fragmentation. These features point to apoptosis.
Grdisa
M
M
Division of Molecular Medicine, Rud-er Bosković Institute, Zagreb, Croatia.
Lopotar
N
N
Pavelić
K
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000473:pathology; D002455:Cell Division / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D009447:Neuroblastoma / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects*; D015645:Tylosin / Q000031:analogs & derivatives* / Q000494:pharmacology
C114856:4-'demycarosyl-20-deoxo-20-(di-N-benzylamino)-8a-aza-8a-homotylosin; D000900:Anti-Bacterial Agents; D000970:Antineoplastic Agents; D015645:Tylosin