title: p53 status in radiation-induced soft-tissue sarcomas.
abstract: BACKGROUND
  Following therapeutic irradiation after a latency period of many years radiation-induced tumors, often sarcomas, can arise. Results of radiation-induced DNA damage can be 1. p53 over-expression, inducing growth arrest or apoptosis, and 2. occurrence of mutations, frequently including the p53 gene, as one molecular promotor for carcinogenesis. We were interested whether radiation-induced sarcomas are associated with alterations of the p53 status.
  
  
  METHODS
  Samples from 11 radiation-induced soft-tissue sarcomas (STS) were studied by a non-radioactive PCR-SSCP sequencing analysis and by immunohistochemistry with five antibodies for their p53 status.
  
  
  RESULTS
  A tumor of one patient possessed a G->A transition in codon 280 (exon 8). Of 11 tumors, 9 showed nuclear p53 positivity, detected by monoclonal antibody DO-1. Of these 9 patients, 7 died during the observation period, whereas the 2 patients with DO-1 negative tumor samples are still alive.
  
  
  CONCLUSIONS
  p53 over-expression and p53 mutation occur in radiation-induced STS. p53 status is expected to have prognostic impact for radiation-induced STS.
authors.affiliation: Institute of Pathology, Martin Luther University of Halle-Wittenberg, Germany. helge.taubert@medizn.uni-halle.de
authors.forename: H
authors.initials: H
authors.lastname: Taubert
authors.forename: A
authors.initials: A
authors.lastname: Meye
authors.forename: M
authors.initials: M
authors.lastname: Bache
authors.forename: R
authors.initials: R
authors.lastname: Hinze
authors.forename: H J
authors.initials: HJ
authors.lastname: Holzhausen
authors.forename: H
authors.initials: H
authors.lastname: Schmidt
authors.forename: F W
authors.initials: FW
authors.lastname: Rath
authors.forename: J
authors.initials: J
authors.lastname: Dunst
authors.forename: P
authors.initials: P
authors.lastname: Würl
mesh_terms: D000328:Adult; D000368:Aged; D005091:Exons; D005260:Female; D016158:Genes, p53; D006801:Humans; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D012509:Sarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000532:radiotherapy*; D016019:Survival Analysis; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000235:genetics
chemical_list: D016159:Tumor Suppressor Protein p53