title: Direct visualisation of individual gene organisation in Trypanosoma brucei by high-resolution in situ hybridisation.
abstract: We achieved the direct visualisation of gene organisation in Trypanosoma brucei using fluorescent in situ hybridisation on extended nuclear DNA fibres. We demonstrated the repetitive nature of the tubulin gene cluster, which consists of up to 19 alpha- and beta-tubulin genes arranged in tandem repeats.
authors.affiliation: School of Biological Sciences, 2.205 Stopford Building, University of Manchester, Manchester M13 9PT, UK. k.ersfeld@man.ac.uk
authors.forename: K
authors.initials: K
authors.lastname: Ersfeld
authors.forename: K
authors.initials: K
authors.lastname: Asbeck
authors.forename: K
authors.initials: K
authors.lastname: Gull
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D016054:DNA, Protozoan / Q000648:ultrastructure*; D017125:Genes, Protozoan; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D015800:Protozoan Proteins / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000648:ultrastructure; D014404:Tubulin / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D015800:Protozoan Proteins; D014404:Tubulin