Trapping of secreted molecules at single-cell level using an HPLC resin particle under a videomicroscope.
Particles of HPLC resins are used for the trapping of secreted molecules from a single cell. The basic molecules, e.g., histamine, are secreted from a single RBL-2H3 cell by granule exocytosis and are trapped by cation-exchange HPLC resins outside the cell. Since quinacrine is concentrated into the exocytotic and acidic microgranules in RBL-2H3 cells, which are used as a model cell line of mast cells, we measured the change in the fluorescence intensity of the quinacrine released from the cells and that of molecules trapped on the resin using a videomicroscope. By measuring the increase in the fluorescence intensity of the resins, we can estimate the real time course of molecular secretion from a single cell.
Masujima
T
T
Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Japan.
Ikeda
K
K
Ozawa
K
K
Tamura
A
A
Tada
Y
Y
Ohya
H
H
Suzuto
M
M
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D003594:Cytoplasmic Granules / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D051381:Rats; D012116:Resins, Plant; D014407:Tumor Cells, Cultured
D012116:Resins, Plant; D000001:Calcimycin