Expression of heat shock protein 47 is increased in remnant kidney and correlates with disease progression.
Glomerulosclerosis is characterized by accumulation of the mesangial extracellular matrix, including type I and V collagen. The processing for the collagens in the glomeruli may play a critical role for development of glomerulosclerosis. We examined the expression of heat shock protein 47 (HSP47), a collagen-binding molecular chaperone in the progressive glomerulosclerosis model. Subtotally nephrectomized rats, unlike sham-operated rats, developed focal and segmental glomerulosclerosis. Immunological staining demonstrated an increased expression of HSP47 which paralleled the expression of type I and IV collagen in the glomeruli of the nephrectomized rats as the glomerulosclerosis developed. The mRNA levels encoding type I and type IV collagen and HSP47 were increased 3.4 fold, 3.6 fold and 2.8 fold, respectively, at week 7 after nephrectomy. By in situ hybridization, the expression of HSP47 mRNA was determined to be localized to the glomeruli with segmental sclerosis. These results suggest that HSP47 may play a central role in the process of extracellular matrix accumulation during the development of glomerulosclerosis.
Sunamoto
M
M
Department of Clinical Bio-Regulatory Science, Faculty of Medicine, Kyoto University, Japan.
Kuze
K
K
Iehara
N
N
Takeoka
H
H
Nagata
K
K
Kita
T
T
Doi
T
T
D000818:Animals; D015153:Blotting, Western; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D018450:Disease Progression; D005109:Extracellular Matrix / Q000473:pathology; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005923:Glomerulosclerosis, Focal Segmental / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D017403:In Situ Hybridization; D007668:Kidney / Q000378:metabolism; D008297:Male; D009392:Nephrectomy; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar
D050958:HSP47 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; C494662:Serpinh1 protein, rat; D003094:Collagen