Determination of plasma oxalate with chloride ion insensitive oxalate oxidase.
A simple colorimetric method has been developed for determination of oxalate in plasma using a C1-insensitive oxalate oxidase purified from grain sorghum leaves. The ultrafiltered plasma collected in 3.5 N HC1 was pretreated with NaNO2 to avoid possible interference by ascorbate. The minimum detection limit of the method is 0.225 mg/l. The % recovery of the added oxalate was 91.5 +/- 5.0 (mean +/- S.D., n = 15). The coefficient of variation within and between batch were < 3 and < 5 respectively. The mean plasma oxalate concentration in healthy subjects was 0.29 mg/l. The method has the advantage over other enzymic methods that it doesn't require the removal of C1- prior to oxalate assay.
Pundir
C S
CS
Kuchhal
N K
NK
Thakur
M
M
Satyapal
D000328:Adult; D002712:Chlorides; D002523:Edible Grain / Q000201:enzymology; D006801:Humans; D007202:Indicators and Reagents; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D010070:Oxalates / Q000097:blood*; D010088:Oxidoreductases / Q000378:metabolism*; D018515:Plant Leaves; D012016:Reference Values; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods
D002712:Chlorides; D007202:Indicators and Reagents; D010070:Oxalates; D010088:Oxidoreductases; C020598:oxalate oxidase