IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.
Mycobacterium avium has become a major human pathogen, primarily due to the emergence of the AIDS epidemic. Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs. The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison. Moreover, by combining international DNA typing results of M. avium complex isolates from a broad spectrum of sources, long-lasting questions on the epidemiology of this major agent of mycobacterial infections will be answered.
van Soolingen
D
D
Diagnostic Laboratory of Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, 3720 BA Bilthoven, The Netherlands. D.van.Soolingen@rivm.nl
Bauer
J
J
Ritacco
V
V
Leão
S C
SC
Pavlik
I
I
Vincent
V
V
Rastogi
N
N
Gori
A
A
Bodmer
T
T
Garzelli
C
C
Garcia
M J
MJ
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology; D000818:Animals; D016172:DNA Fingerprinting; D004251:DNA Transposable Elements; D016208:Databases, Factual; D004197:Disease Reservoirs; D006801:Humans; D009162:Mycobacterium avium / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D012703:Serotyping / Q000379:methods; D012984:Software; D014376:Tuberculosis / Q000175:diagnosis* / Q000662:veterinary
D004251:DNA Transposable Elements