Rapid diagnosis of pulmonary tuberculosis with the LCx Mycobacterium tuberculosis assay and comparison with conventional diagnostic techniques.
The LCx MTB amplification assay is a nucleic acid amplification test intended for the direct detection of Mycobacterium tuberculosis complex in respiratory specimens. We evaluated its performance on 2,001 consecutive respiratory specimens; 78 were culture positive for M. tuberculosis. Sensitivity, specificity, and positive and negative predictive values of this assay for all specimens compared to culture results were 88.5, 97.7, 60.5, and 99.5%, respectively. When referred to resolved clinical diagnosis of active tuberculosis, these values improved to 90.2, 98.4, 72.8, and 99.5%, respectively.
Rohner
P
P
Infectious Disease Division, Bacteriology Laboratory, University Hospital Geneva, 1211 Geneva, Switzerland. Peter.Rohner@hcuge.ch
Jahn
E I
EI
Ninet
B
B
Ionati
C
C
Weber
R
R
Auckenthaler
R
R
Pfyffer
G E
GE
D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D005750:Gastric Juice / Q000382:microbiology; D006801:Humans; D007202:Indicators and Reagents; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013183:Sputum / Q000382:microbiology; D014132:Trachea / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis*
D004269:DNA, Bacterial; D007202:Indicators and Reagents; D011933:Reagent Kits, Diagnostic