IL-8 is one of the major chemokines produced by monkey airway epithelium after ozone-induced injury.
A rhesus monkey interleukin (IL)-8 cDNA clone with >94% homology to the human IL-8 gene was isolated by differential hybridization from a cDNA library of distal airways after ozone inhalation. In situ hybridization and immunohistochemistry showed increased IL-8 mRNA and protein levels in epithelial cells at 1 h but not at 24 h after inhalation of ozone. The appearance of IL-8 in airway epithelial cells correlated well with neutrophil influx into airway epithelia and lumens. Air-liquid interface cultures of tracheobronchial epithelial cells were exposed to ozone in vitro. We observed a transient increase in IL-8 secretion in culture medium immediately after ozone exposure and a dose-dependent increase in IL-8 secretion and mRNA production. In vitro neutrophil chemotaxis showed a parallel dose and time profile to epithelial cell secretion of IL-8. Treatment with anti-IL-8 neutralizing antibody blocked >80% of the neutrophil chemotaxis in vitro. These results suggest that IL-8 is a key chemokine in acute ozone-induced airway inflammation in primates.
Chang
M M
MM
Center for Comparative Respiratory Biology and Medicine, University of California, Davis, California 95616, USA.
Wu
R
R
Plopper
C G
CG
Hyde
D M
DM
D000280:Administration, Inhalation; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001483:Base Sequence; D001980:Bronchi / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D018076:DNA, Complementary; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D015723:Gene Library; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D008168:Lung / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010126:Ozone / Q000008:administration & dosage / Q000633:toxicity*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
D000911:Antibodies, Monoclonal; D018076:DNA, Complementary; D016209:Interleukin-8; D010126:Ozone