The P22 Erf protein and host RecA provide alternative functions for transductional segregation of plasmid-borne duplications.
A tandem DNA duplication carried on a ColE1-derived plasmid segregates at high frequency upon generalized transduction by phage P22 HT. Transductional segregation of the plasmid-borne duplication can be promoted either by RecA or by the Erf function of P22, indicating that transductional segregation is a consequence of the recombination events that re-circularize the plasmid in the recipient cell. RecA-mediated and Erf-mediated transduction give similar frequencies of duplication segregation and yield the same types of segregation products, indicating that two distinct recombination machineries (RecA + RecBCD and Erf + RecBCD) perform similar or identical recombination reactions on plasmid DNA substrates transduced by bacteriophage P22 HT.
Garzón
A
A
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Spain.
Cano
D A
DA
Casadesús
J
J
D017100:Bacteriophage P22 / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication; D004721:Endoplasmic Reticulum; D010957:Plasmids / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D011938:Rec A Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic / Q000502:physiology; D012486:Salmonella typhimurium / Q000235:genetics / Q000821:virology; D014161:Transduction, Genetic / Q000502:physiology*
D011938:Rec A Recombinases