Intrahepatic flow disturbance as detected by in vivo acridine orange staining in endothelin-1-treated and cirrhotic rats.
Intrahepatic flow in rats was examined by systemic acridine orange (AO) labeling and succeeding microscopic observations of post-fixed livers. In controls, all periportal areas were stained evenly throughout the liver mass, and the staining often branched between the liver lobules in a acinous fashion. Intraportal endothelin-1 infusion caused a marked heterogenous staining; strong staining around large portal vein branches in the central portion of the liver, but much less in the periphery. Cirrhotic livers exhibited overall weaker staining intensity with no accentuation in periportal areas and restricted radial staining within pseudo-lobules. AO infusion may help detect hepatic flow disturbance in vivo.
Masuda
Y
Y
Division of Toxicology, Niigata College of Pharmacy, Japan.
Oguma
T
T
Kaneko
H
H
D000165:Acridine Orange; D000818:Animals; D002251:Carbon Tetrachloride; D019332:Endothelin-1 / Q000494:pharmacology*; D005268:Femoral Vein; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000503:physiopathology; D008102:Liver Circulation / Q000187:drug effects* / Q000502:physiology*; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000503:physiopathology*; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000473:pathology / Q000503:physiopathology; D008856:Microscopy, Fluorescence; D010634:Phenobarbital; D017082:Portal Pressure / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
D019332:Endothelin-1; D002251:Carbon Tetrachloride; D000165:Acridine Orange; D010634:Phenobarbital