The INSL4 gene maps close to WI-5527 at 9p24.1-->p23.3 clustered with two relaxin genes and outside the critical region for the monosomy 9p syndrome.
The insulin like growth factor 4 (INSL4) gene belongs to the insulin gene superfamily and has been mapped by fluorescent in situ hybridization to 9p24. Expression of INSL4, of unknown function, has been recently detected in the perichondrium and ligaments of the human embryo. Here we have mapped INSL4 within the framework of a partial YAC contig covering the distal part of 9p to find out whether this gene lies within the critical region defined for the monosomy 9p syndrome. The gene was also located using a human-rodent radiation hybrid panel. INSL4 was found to be distal to the del(9p) critical region and excluded as a candidate for the syndrome. In addition, the positions of two relaxin genes (RLN1, RLN2) that belong to the same superfamily as INSL4 and which have also been mapped to chromosome 9, were refined. We have shown that the three genes are clustered in the same region.
Veitia
R
R
Unité d'Immunogénétique Humaine, Institut Pasteur; Descartes-Paris V, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Paris, France. rveitia@pasteur.fr
Laurent
A
A
Quintana-Murci
L
L
Ottolenghi
C
C
Fellous
M
M
Vidaud
M
M
McElreavey
K
K
D000818:Animals; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9; D017931:DNA Primers; D004622:Embryo, Mammalian; D005819:Genetic Markers; D006133:Growth Substances; D006801:Humans; D006822:Hybrid Cells; D036341:Intercellular Signaling Peptides and Proteins; D009006:Monosomy / Q000235:genetics*; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011257:Pregnancy Proteins / Q000235:genetics*; D012065:Relaxin / Q000235:genetics*; D012377:Rodentia; D013577:Syndrome
D017931:DNA Primers; D005819:Genetic Markers; D006133:Growth Substances; C101126:INSL4 protein, human; D036341:Intercellular Signaling Peptides and Proteins; D011257:Pregnancy Proteins; D012065:Relaxin