Validation of an EIA kit for determination of total thyroid hormones in rat serum. Effects of different anaesthetics.
Two enzyme immunoassays for the quantitative determination of total thyroxine (T4) and total triiodothyronine (T3) in human serum were validated to measure total T4 and T3 in rat serum. The specificity, sensitivity, detectability and reproducibility have been tested as well as the effects of different anaesthetics (pentobarbital and a mixture of ketamine and xylacine) on serum thyroid hormone levels. Hormones were quantified, by means of a previously validated technique, 18 hours after surgery for the placement of a stem for blood extraction in conscious and freely moving animals. Thyroid serum levels, especially T3, were slightly lower in xylacine plus ketamine treated animals than in those treated with pentobarbital. The administration of glutamic acid, stimulated the secretion of thyroid hormones, this effect appearing 30 minutes after its administration and it was independent of the anaesthetic used during the surgery for the cannula placement, although in pentobarbital treated rats, the serum T3 level increase induced by glutamate appears 60 minutes after the treatment.
Alfonso
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Departamento de Biología Funcional y Ciencias de la Salud, Laboratorio de Fisiología Animal, Facultad de Ciencias, Universidad de Vigo, Spain. pallares@setei.uvigo.es
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D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D010424:Pentobarbital / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013974:Thyroxine / Q000097:blood*; D014284:Triiodothyronine / Q000097:blood*; D014991:Xylazine / Q000494:pharmacology
D000777:Anesthetics; D011933:Reagent Kits, Diagnostic; D014284:Triiodothyronine; D014991:Xylazine; D018698:Glutamic Acid; D007649:Ketamine; D010424:Pentobarbital; D013974:Thyroxine