Long-term detection of microchimaerism in peripheral blood after pretransplantation blood transfusion.
Renal allograft survival is prolonged after pretransplantation blood transfusion. The aim of this study was to test retrospectively the development and persistence of microchimaerism after pretransplantation blood transfusion and to assess whether the type of blood transfusion (partially matched [= sharing of at least one HLA-B and one HLA-DR antigen between blood donor and recipient] versus mismatched) influences the (continued) presence of donor-type cells. A sensitive nested PCR technique based on HLA-DRB1 allele-specific amplification using sequence-specific primers (detection level: one donor cell among 10(5) recipient cells) for detection of donor cells was implemented in our laboratory. We studied 21 patients for microchimaerism in the peripheral blood compartment, following blood transfusion. Our preliminary data show that microchimaerism was detectable up to 8 weeks after blood transfusion. In all patients receiving a partially matched blood transfusion, donor-type cells were detected in the first week after transfusion, in 7/8 patients 2-4 weeks after transfusion, and in some patients up to 8 weeks after transfusion. After mismatched transfusion a tendency to shorter duration of microchimaerism was observed.
Vervoordeldonk
S F
SF
Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, University of Amsterdam.
Doumaid
K
K
Remmerswaal
E B
EB
ten Berge
I J
IJ
Wilmink
J M
JM
de Waal
L P
LP
Boog
C J
CJ
D001803:Blood Transfusion; D002678:Chimera; D005260:Female; D015803:HLA-DR3 Antigen / Q000097:blood; D015809:HLA-DR7 Antigen / Q000097:blood; D006650:Histocompatibility Testing; D006801:Humans; D016030:Kidney Transplantation; D008297:Male; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D013997:Time Factors
D015803:HLA-DR3 Antigen; D015809:HLA-DR7 Antigen