title: [A number of Vibrio cholerae non-O1 isolated from aquatic environments].
abstract: To estimated existence of Vibrio cholerae non-O1 in aquatic environments, the organisms isolated from river, estuary and sea water. V. cholerae non-O1 isolated form midstream and estuary water could be counted from 1.6 to 2400 CFU/100 ml by the membrane filtrated method (MF). V. cholerae non-O1 existed in midstream water more than in estuary water. However, the isolated organisms from estuary rate by MF (37.5%) was lower than it by alkaline peptone enrichment medium method (AP) (75.0%), as a result of halophilic bacteria grow an selected medium of MF. And the number of V. cholerae non-O1 isolated from aquatic environment did not correlate environmental parameters. The number of V. cholerae non-O1 isolated from river water varied, it suggested that the organism collectively adhere a floating matter. V. cholerae non-O1 was not detected in 500 ml sea water by AP and MF method. These results conclude that V. cholerae non-O1 exist in river water more than in sea.
authors.affiliation: Laboratory of Public Health, School of Allied Health Sciences, Kitasato University.
authors.forename: H
authors.initials: H
authors.lastname: Uchiyama
mesh_terms: D002452:Cell Count; D007564:Japan; D014734:Vibrio cholerae / Q000302:isolation & purification*; D014871:Water Microbiology