The Pad1+ gene encodes a subunit of the 26 S proteasome in fission yeast.
We have isolated a fission yeast mutant, mts5-1, in a screen for mutations that confer both methyl 2-benzimidazolecarbamate resistance (MBCR) and temperature sensitivity (ts) on Schizosaccharomyces pombe. This screen has previously isolated mutations in the 26 S proteasome subunits Mts2, Mts3, and Mts4. We show that the mutation in the mts5-1 strain occurs in the pad1(+) gene. pad1(+) was originally isolated on a multicopy plasmid that was capable of conferring staurosporine resistance on a wild type strain. mts5-1/pad1-1 has a similar phenotype to 26 S proteasome mutants previously isolated in the same screen and we show that Pad1 interacts genetically with two of these subunits, Mts3 and Mts4. In this study we describe the identification of Pad1 as a subunit of the 26 S proteasome in fission yeast.
Penney
M
M
MRC Human Genetics Unit Western General Hospital, Edinburgh EH4 2XU Scotland, United Kingdom.
Wilkinson
C
C
Wallace
M
M
Javerzat
J P
JP
Ferrell
K
K
Seeger
M
M
Dubiel
W
W
McKay
S
S
Allshire
R
R
Gordon
C
C
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D012568:Schizosaccharomyces / Q000235:genetics* / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D014452:Ubiquitins / Q000378:metabolism
D018076:DNA, Complementary; D005656:Fungal Proteins; D011994:Recombinant Proteins; D029702:Schizosaccharomyces pombe Proteins; D015534:Trans-Activators; D014452:Ubiquitins; C093857:rpn11 protein, S pombe; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease