title: Direct effects of corticotrophin on oral keratinocyte cell proliferation.
abstract: Corticotropin is produced by keratinocytes and may have an immunoregulatory role in oral mucosa and skin. We have investigated its effects on a human oral keratinocyte cell line and shown that corticotropin, acting via its specific receptor, stimulates a dose-dependent increase in DNA synthesis and induces cell proliferation. When cells were incubated in the presence of increasing concentrations of corticotropin, there were significant increases in intracellular cAMP levels. Corticotropin-stimulated mitogenesis and cell proliferation were attenuated by the adenylyl cyclase inhibitor SQ22,536, but were unaffected by inhibitors of protein kinase C or tyrosine kinase. These data identify corticotropin as a mitogenic regulatory peptide of keratinocytes acting via cAMP.
authors.affiliation: Molecular Signalling Group, Oral Diseases Research Centre, St Bartholomew's & the Royal London School of Medicine & Dentistry, UK. s.kapas@mds.qmw.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Kapas
authors.forename: E
authors.initials: E
authors.lastname: Hagi-Pavli
authors.forename: D W
authors.initials: DW
authors.lastname: Brown
authors.forename: V
authors.initials: V
authors.lastname: Chhajlani
authors.forename: P M
authors.initials: PM
authors.lastname: Farthing
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000067956:Adenylyl Cyclase Inhibitors; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects; D009061:Mouth Mucosa / Q000166:cytology / Q000187:drug effects*; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D018043:Receptors, Corticotropin / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000067956:Adenylyl Cyclase Inhibitors; D018043:Receptors, Corticotropin; C017759:9-(tetrahydro-2-furyl)-adenine; D003994:Bucladesine; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP; D011505:Protein-Tyrosine Kinases; D011493:Protein Kinase C; D000225:Adenine