A temperate phage with cohesive ends induced by mitomycin C treatment of Lactobacillus casei.
A temperate phage, named PL-2, was induced from Lactobacillus casei ATCC 27092 by mitomycin C treatment of the cells at exponential growth phase. The phage had an isometric head of 45 nm in diameter and a flexible, non-contractile tail, 150 nm long and 10 nm wide, with a sharp tip. Along the tail axis, about 40 regularly spaced striae were seen. The phage DNA had complementary cohesive ends. The restriction enzyme map of the DNA was constructed by using 13 different restriction endonucleases. The size of the DNA was 35.2 kb, 83% in size of that of phage PL-1 lytic for the same Lb. casei strain.
Nakashima
Y
Y
Microbiology Laboratory, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
Hasuwa
H
H
Kakita
Y
Y
Murata
K
K
Kuroiwa
A
A
Miake
F
F
Watanabe
K
K
D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007780:Lacticaseibacillus casei / Q000187:drug effects / Q000821:virology*; D016685:Mitomycin / Q000494:pharmacology*
D004279:DNA, Viral; D016685:Mitomycin