Studies in vivo of omega-gliadins in gluten sensitivity (coeliac sprue disease).
1. Highly purified omega-gliadins from wheat were used to challenge gluten-sensitized individuals. Characteristic responses by mucosal CD3(+) and gamma delta+ lymphocytes were demonstrated. Each lymphocyte subset showed an increase within 8-12 h post-challenge, indicating a specific response by the rectal mucosa to this gliadin species.2. Available sequence data for the omega-gliadins and homologous proteins from barley and rye indicate a common repeating octapeptide motif (consensus PQQPFPQQ). The results indicate, therefore, that the octapeptide repeat, or a contained sequence such as PQQP, plays an important role in the mucosal immunopathology of gluten sensitivity.
Ensari
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University Department of Medicine, Clinical Sciences Building, Hope Hospital, Stott Lane, Salford M6 8HD, U.K.
Marsh
M N
MN
Moriarty
K J
KJ
Moore
C M
CM
Fido
R J
RJ
Tatham
A S
AS
D017252:CD3 Complex / Q000032:analysis; D002446:Celiac Disease / Q000276:immunology*; D002852:Chromatography, Ion Exchange; D016384:Consensus Sequence; D005903:Gliadin / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000276:immunology*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D012007:Rectum / Q000276:immunology*
D017252:CD3 Complex; D016692:Receptors, Antigen, T-Cell, gamma-delta; D005903:Gliadin