title: Photoaffinity labeling of PKC isozymes by phorbol ester derivatives.
abstract: Photoaffinity probes PPDA and PPTD, which have diazoacetyl and trifluorodiazopropionyl group at C-13 position in phorbol, respectively, were synthesized. Photoaffinity labeling of protein kinase C isozymes by both the probes resulted in specific cross-linking.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Uotsu
authors.forename: M
authors.initials: M
authors.lastname: Sodeoka
authors.forename: M
authors.initials: M
authors.lastname: Shibasaki
mesh_terms: D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D003979:Diazonium Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D007527:Isoenzymes / Q000737:chemistry / Q000378:metabolism / Q000528:radiation effects; D010704:Phorbols / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D019920:Photoaffinity Labels / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D011493:Protein Kinase C / Q000737:chemistry* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D003432:Cross-Linking Reagents; D003979:Diazonium Compounds; D007527:Isoenzymes; D010704:Phorbols; D019920:Photoaffinity Labels; C115103:phorbol 12-pyrenebutyrate 13-(3,3,3-trifloro-2-diazopropioate); D011493:Protein Kinase C