Developmental changes and functional properties of human memory T cell subpopulations defined by CD60 expression.
The present study was undertaken to examine developmental changes of T cells expressing CD60 and their functional properties. Three-color immunofluorescence analysis revealed that the CD60 antigen was preferentially expressed on a proportion of memory (CD45RO+) CD4+ T cells, but less on memory CD8+ T cells, while this antigen is undetectable in naive (CD45RO-) T cells. A frequency of memory CD4+ T cells expressing CD60 in the peripheral blood was negligible in newborns and gradually increased with advancing age. CD60+ memory CD4+ T cells showed stronger proliferative responses to PPD and produced higher levels of IL-4 and IL-10 than CD60- ones, whereas production of IL-2 and IFN-gamma was similarly found in both cell subpopulations. In addition, it was shown that efficient helper activity for Ig production by B cells was predominated in CD60+ memory CD4+ T cells. These results suggest that CD60 may be primarily expressed on the functionally differentiated memory effector cells among circulating CD45RO+ CD4+ T cells.
Wada
T
T
Department of Pediatrics, Kanazawa University, Japan.
Seki
H
H
Konno
A
A
Ohta
K
K
Nunogami
K
K
Kaneda
H
H
Kasahara
Y
Y
Yachie
A
A
Koizumi
S
S
Taniguchi
N
N
Miyawaki
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000032:analysis*; D015496:CD4-Positive T-Lymphocytes / Q000737:chemistry*; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005732:Gangliosides / Q000032:analysis*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D007156:Immunologic Memory; D007223:Infant; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000032:analysis
D015703:Antigens, CD; D016207:Cytokines; D005732:Gangliosides; D007136:Immunoglobulins; C026226:ganglioside, GD3; D017493:Leukocyte Common Antigens