Effects of angiogenic growth factors and a penetrance enhancer on composite grafts.
Skin-cartilage composite grafts are invaluable tissues used in facial reconstruction, yet their survival is unpredictable beyond a 1-cm diameter. In this study, the angiogenic growth factors basic fibroblast growth factor (bFGF) and endothelial cell growth factor (ECGF) and a penetrance enhancer (dimethyl sulfoxide [DMSO]) were applied to composite grafts to determine their effects on survival and vascularization. We applied ECGF, bFGF, and DMSO either topically or by intradermal injection to 120 auricular composite grafts (3.0 cm diameter) in New Zealand White rabbits. Dermabrasion was performed in 2 groups to attempt to increase transdermal delivery. Graft viability and vascularity were evaluated 3 weeks later by template analysis and angiography. In the results, ECGF and bFGF, when grouped together, had a 40% increase in vascular ingrowth as compared to controls (p < .001). However, neither ECGF nor bFGF increased graft survival. A coincidental finding was that DMSO with dermabrasion significantly improved graft viability (>100%) with or without an angiogenic agent (p < .02). The potential of DMSO with dermabrasion to increase composite graft viability warrants further investigation.
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Department of Otolaryngology-Head and Neck Surgery, University of Minnesota School of Medicine, Hennepin County Medical Center, Minneapolis 55455, USA.
Winters
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D000287:Administration, Topical; D000792:Angiography; D000818:Animals; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016228:Endothelial Growth Factors / Q000494:pharmacology*; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D006085:Graft Survival / Q000187:drug effects*; D006801:Humans; D007271:Injections, Intradermal; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011817:Rabbits; D016038:Skin Transplantation / Q000502:physiology*; D013524:Surgical Flaps / Q000098:blood supply*
D016228:Endothelial Growth Factors; D016222:Fibroblast Growth Factor 2; D004121:Dimethyl Sulfoxide