Yeast as a model organism for studying the actions of DNA topoisomerase-targeted drugs.
The budding yeast Saccharomyces cerevisiae has been exploited to investigate the cytotoxic mechanisms of drugs that target DNA topoisomerases. This model organism has been used to establish eukaryotic DNA topoisomerase I or II as the cellular target of specific antineoplastic agents, to define mutations in these enzymes that confer drug resistance and to elucidate the cellular factors that modulate cell sensitivity to DNA topoisomerase-targeted drugs. These findings have provided valuable insights into the critical activities of these enzymes and how perturbing their functions produces DNA damage and cell death.
Reid
R J
RJ
Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, 233 S. 10th St., Philadelphia, PA 19107, USA.
Benedetti
P
P
Bjornsti
M A
MA
D000970:Antineoplastic Agents / Q000633:toxicity; D016923:Cell Death / Q000235:genetics; D004249:DNA Damage / Q000235:genetics; D004264:DNA Topoisomerases, Type I / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D004791:Enzyme Inhibitors / Q000633:toxicity*; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors
D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D004264:DNA Topoisomerases, Type I