A rapid method for the identification and partial serotyping of Listeria monocytogenes in food by PCR and restriction enzyme analysis.
Two highly specific primers for Listeria monocytogenes were used to yield from foods such as milk, soft cheese and meat, PCR products that were cleaved with the restriction enzyme HindIII. The fragments generated allowed a distinction between two groups of L. monocytogenes serovars: serovars 1/2a and 1/2c cluster in one group and serovars 1/2b, 3b and 4b in the other subgroup. Since this procedure can be completed in 24 h, an epidemiological association between human disease and suspected sources can be rapidly confirmed at the subgroup level in the laboratory.
Manzano
M
M
Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
Cocolin
L
L
Cantoni
C
C
Comi
G
G
D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D005516:Food Microbiology; D006801:Humans; D008089:Listeria monocytogenes / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D008460:Meat / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping / Q000379:methods; D013552:Swine
D017931:DNA Primers; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes