New method for determination of fecal sterols in urine using non-chlorinated solvents.
A new method has been developed to determine a number of sterols in urine using non-chlorinated solvents, namely methyl tert.-butyl ether and methanol (the MTB method). The method involves liquid-liquid extraction, saponification, reextraction, silylation and final identification and quantification by GC-FID. The sterols determined were coprostanol, epicoprostanol, cholesterol and dihydrocholesterol. 5Alpha-cholestane was used as internal standard. The limit of detection for the sterols in this experiment was 2 microg l(-1) urine. Recovery of coprostanol over the range 5-100 microg l(-1) urine by this method was between 80 and 92%.
Börjesson
E
E
Swedish University of Agricultural Science, Department of Microbiology, Uppsala.
Sundin
A
A
Leeming
R
R
Torstensson
L
L
D004083:Cholestanol / Q000652:urine; D005243:Feces / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D000432:Methanol; D008738:Methyl Ethers; D012680:Sensitivity and Specificity; D012722:Sewage / Q000032:analysis*; D012997:Solvents; D013261:Sterols / Q000652:urine*
D008738:Methyl Ethers; D012722:Sewage; D012997:Solvents; D013261:Sterols; C043243:methyl tert-butyl ether; D004083:Cholestanol; D000432:Methanol