title: The mouse Y chromosome interval necessary for spermatogonial proliferation is gene dense with syntenic homology to the human AZFa region.
abstract: The Delta Sxrb deletion interval of the mouse Y chromosome contains Spy, a spermatogenesis factor gene(s) whose expression is essential for the postnatal development of the mitotic germ cells, spermatogonia. The boundaries of Delta Sxrb are defined by the duplicated genes Zfy1 and Zfy2 and four further genes have previously been mapped within the interval: Ube1y and Smcy, linked with Zfy1 on a contig of 250 kb, and Dffry and Uty, which were unanchored. The interval was estimated to be >450 kb. In order to identify any further gene(s) that may underlie Spy, systematic exon trapping was performed on an extended contig, anchored on Zfy1, which covers 750 kb of the Delta Sxrb interval. Exons from two novel genes were isolated and placed together with Dffry and Uty on the contig in the order Dffry-Dby-Uty-Tspy-Eif2gammay-Smcy- Ube1y-Zfy1. All the genes, with the double exception of Tspy, are X-Y homologous and produce putatively functional, spliced transcripts. The tight linkage and order of Dffry, Dby and Uty was shown to be conserved in deletion intervals 5C/5D of the human Y chromosome by the construction of a contig of human PAC and YAC clones; this represents the first example of syntenic homology between Y chromosomes from two distinct mammalian orders. Interval 5C/5D contains the distal boundary of the AZFa interval, which, like Delta Sxrb, is believed to be necessary for spermatogonial development in the prepubertal testis. Our results therefore show that AZFa and Spy may be encoded by homologous genes.
authors.affiliation: INSERM U491, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille cedex 5, France.
authors.forename: S
authors.initials: S
authors.lastname: Mazeyrat
authors.forename: N
authors.initials: N
authors.lastname: Saut
authors.forename: C A
authors.initials: CA
authors.lastname: Sargent
authors.forename: S
authors.initials: S
authors.lastname: Grimmond
authors.forename: G
authors.initials: G
authors.lastname: Longepied
authors.forename: I E
authors.initials: IE
authors.lastname: Ehrmann
authors.forename: P S
authors.initials: PS
authors.lastname: Ellis
authors.forename: A
authors.initials: A
authors.lastname: Greenfield
authors.forename: N A
authors.initials: NA
authors.lastname: Affara
authors.forename: M J
authors.initials: MJ
authors.lastname: Mitchell
mesh_terms: D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002876:Chromosomes, Bacterial; D002877:Chromosomes, Human; D053487:DEAD-box RNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D005260:Female; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008025:Ligases / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens; D008969:Molecular Sequence Data; D009687:Nuclear Proteins; D011506:Proteins / Q000235:genetics; D051381:Rats; D013091:Spermatogenesis / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic; D044767:Ubiquitin-Protein Ligases; D014998:Y Chromosome
chemical_list: D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D015778:Minor Histocompatibility Antigens; D009687:Nuclear Proteins; D011506:Proteins; D014157:Transcription Factors; C484453:UTY protein, human; C102450:Uty protein, mouse; C054430:ZFY protein, human; C501342:Zfy1 protein, mouse; C501343:Zfy2 protein, mouse; D044767:Ubiquitin-Protein Ligases; C492034:DDX3Y protein, human; C115600:Ddx3y protein, mouse; D053487:DEAD-box RNA Helicases; D008025:Ligases