title: Immunohistochemical and ultrastructural evidence of hog cholera virus infection of megakaryocytes in bone marrow and spleen.
abstract: Twelve pigs were inoculated with a highly virulent strain of hog cholera virus (HCV) to study viral infection of megakaryocytes in the bone marrow and spleen. Immunohistochemical and ultrastructural examination revealed HCV infection in a small proportion (2.5-9.0%) of these cells from the 2nd to the 9th day after inoculation, at which time the experiment was terminated. Megakaryocyte infection accounts for the presence of viral antigens in platelets. The latter may represent a passive vehicle for spreading the virus in the animal.
authors.affiliation: Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
authors.forename: J C
authors.initials: JC
authors.lastname: Gómez-Villamandos
authors.forename: E
authors.initials: E
authors.lastname: Ruiz-Villamor
authors.forename: F J
authors.initials: FJ
authors.lastname: Salguero
authors.forename: M J
authors.initials: MJ
authors.lastname: Bautista
authors.forename: L
authors.initials: L
authors.lastname: Carrasco
authors.forename: C
authors.initials: C
authors.lastname: Sánchez
authors.forename: M
authors.initials: M
authors.lastname: Quezada
authors.forename: M A
authors.initials: MA
authors.lastname: Sierra
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001853:Bone Marrow / Q000473:pathology* / Q000821:virology; D002452:Cell Count; D006691:Classical Swine Fever / Q000473:pathology / Q000821:virology*; D006692:Classical Swine Fever Virus / Q000502:physiology* / Q000648:ultrastructure*; D005260:Female; D007124:Immunoenzyme Techniques / Q000662:veterinary; D002479:Inclusion Bodies / Q000648:ultrastructure; D008297:Male; D008533:Megakaryocytes / Q000648:ultrastructure / Q000821:virology*; D010976:Platelet Count / Q000662:veterinary; D013154:Spleen / Q000473:pathology* / Q000821:virology; D013552:Swine
chemical_list: D000956:Antigens, Viral