Chromosome abnormalities in peripheral blood lymphocytes from Macaca fascicularis and Erythrocebus patas (Cercopithecidae, Catarrhini) after X-ray irradiation.
In this paper, we describe the results of a qualitative and quantitative study of chromosomal reorganizations in X-irradiated (1 Gy and 2 Gy) lymphocytes from Macaca fascicularis (MFA) and Erythrocebus patas (EPA). A total of 515 breakpoints in M. fascicularis and 271 breakpoints in E. patas have been detected, identified and localized in the ideogram of the species. The Chi square test indicates that the distribution of breakpoints along the chromosomes is not random in M. fascicularis, and is not random for the p and q arms and bands in both species. Chromosome 5 of M. fascicularis (MFA5), chromosome 1 of E. patas (EPA1), and chromosome arms MFA5q, and EPA 1p are significantly more affected than expected, while chromosome MFA9 is less affected. Terminal regions of chromosome arms accumulate a higher number of breakpoints than the rest of the chromosome (44.4% in M. fascicularis and 45.98% in E. patas); 92.06% and 91.97% of breakpoints are observed in G negative bands in M. fascicularis and E. patas, respectively.
Borrell
M
M
Department de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Ponsà
M
M
Egozcue
J
J
Rubio
A
A
Garcia
M
M
D000818:Animals; D002871:Chromosome Banding; D019457:Chromosome Breakage; D002875:Chromosomes / Q000528:radiation effects / Q000648:ultrastructure; D004901:Erythrocebus patas / Q000097:blood / Q000235:genetics*; D005260:Female; D066298:In Vitro Techniques; D007621:Karyotyping; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D008252:Macaca fascicularis / Q000097:blood / Q000235:genetics*; D008297:Male; D013045:Species Specificity; D014178:Translocation, Genetic