A human RNA polymerase II transcription termination factor is a SWI2/SNF2 family member.
We obtained protein sequence information from Drosophila factor 2, an ATP-dependent RNA polymerase II transcription termination factor, and discovered that it was identical to a SWI2/SNF2 family member called lodestar. Portions of putative human and Caenorhabditis elegans homologues were found in the sequence data bases and a complete cDNA for the human factor was generated using polymerase chain reaction techniques. Recombinant human factor 2 was produced in a baculovirus expression system, purified, and characterized. Similar to the authentic Drosophila factor, the human factor displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity. Both factors were able to work on elongation complexes from either species. We discuss the mechanism of termination by factor 2 and the implications for the role of factor 2 in cellular activities.
Liu
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Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA.
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Price
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D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism; D003001:Cloning, Molecular; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000737:chemistry; D016208:Databases, Factual; D004330:Drosophila / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins; D012319:RNA Polymerase II / Q000235:genetics*; D011994:Recombinant Proteins / Q000737:chemistry; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000737:chemistry*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C499336:SMARCA1 protein, human; C084011:SMARCA2 protein, human; D014157:Transcription Factors; D012319:RNA Polymerase II; D000251:Adenosine Triphosphatases; C084108:SMARCA4 protein, human; D004265:DNA Helicases