Molecular organization of the gene encoding Xenopus laevis transforming growth factor-beta 5.
Transforming growth factor-beta s (TGF-beta s) are multifunctional polypeptides, known to influence proliferation and differentiation of many cell types. TGF-beta 5 cDNA was cloned from Xenopus laevis and this isoform is unique to the amphibians. Here, we report the isolation and characterization of the TGF-beta 5 genomic clones to determine the structure of TGF-beta 5 gene. The gene consists of seven exons and all intron-exon boundaries follow the GT-AG consensus. The organization of TGF-beta 5 gene was identical to that of the mammalian TGF-beta isoforms, with the exception of position of the first splice junction. We determined the size of TGF-beta 5 gene to be approximately 20 kb.
Vempati
U D
UD
Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science, Bangalore, India.
Kondaiah
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005091:Exons; D005796:Genes; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000235:genetics*; D014982:Xenopus laevis / Q000235:genetics*
D016212:Transforming Growth Factor beta