Formation of malonaldehyde in the presence of probucol, an anti-atherosclerosis drug.
Formation and inhibition of malonaldehyde (MA) from blood plasma lipids oxidized by Fenton's reagent in the absence or presence of probucol [4,4'-(isopropylidenedithio)bis(2,6-di-tert-butylphenol)] and L-ascorbic acid were investigated. The amount of MA formed was quantitatively analysed by gas chromatography. L-Ascorbic acid inhibited MA formation by about 30% at the level of 4.0/micromol, but the amount of MA formed was increased by the presence of probucol. When 3.0 micromol oxidized probucol was hydrolysed at pH 1. 3 and 5, 2616.5 nmol, 287.5 nmol and 103.9 nmol MA were recovered, respectively. This is the first report of quantitative analysis of MA formed from probucol on oxidation.
Miyake
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T
Department of Environmental Toxicology, University of California, Davis 95616, USA.
Shibamoto
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T
D000818:Animals; D000924:Anticholesteremic Agents / Q000097:blood / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D004347:Drug Interactions; D006736:Horses; D006861:Hydrogen Peroxide; D066298:In Vitro Techniques; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000032:analysis* / Q000097:blood; D011341:Probucol / Q000097:blood / Q000737:chemistry / Q000494:pharmacology*
D000924:Anticholesteremic Agents; D000975:Antioxidants; C045076:Fenton's reagent; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D007501:Iron; D011341:Probucol; D001205:Ascorbic Acid