Promoter characterization of the human Na+/I- symporter.
The Na+/I- symporter (NIS) is the plasma membrane protein that mediates active iodide uptake into thyroid follicular cells. To understand the molecular mechanisms of human NIS (hNIS) gene regulation, the 2-kb immediate 5'-flanking region of hNIS was characterized. The tentative hNIS transcriptional start site was mapped to -375 nucleotide relative to the ATG site. Various 5'-genomic DNA fragments were tested for promoter activity in thyroid and nonthyroid cells. Our results indicate that the DNA regulatory elements in the 2-kb immediate 5'-flanking region were not sufficient to confer thyroid-selective transcription of the hNIS gene. In addition, hNIS minimal promoter was localized to a region of 144 bp that contains a 90-bp stretch of a highly conserved DNA fragment between hNIS and rat NIS.
Ryu
K Y
KY
Department of Physiology, Ohio State University, Columbus 43210, USA.
Tong
Q
Q
Jhiang
S M
SM
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis / Q000737:chemistry; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D007454:Iodides / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D051381:Rats; D012045:Regulatory Sequences, Nucleic Acid; D015183:Restriction Mapping; D027981:Symporters; D013961:Thyroid Gland / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D014162:Transfection; D000099209:Sodium-Iodide Symporters
D002352:Carrier Proteins; D004247:DNA; D007454:Iodides; D008565:Membrane Proteins; D027981:Symporters; D014157:Transcription Factors; D000099209:Sodium-Iodide Symporters