title: In vivo tracking and protective properties of Yersinia-specific intestinal T cells.
abstract: After invasion via M cells enteropathogenic Yersinia enterocolitica subsequently establish an infection at three different sites: (i) Peyer's patches (PP), (ii) mesenteric lymph nodes (MLN), and after systemic dissemination in (iii) spleen, liver and lung. In order to characterize protective properties of intestinal T cells at the different sites of Y. enterocolitica infection, PP and MLN T cells were isolated from Y. enterocolitica-infected C57B1/6 mice and Yersinia-specific T cell lines were generated. These T cells exhibited the phenotype of CD4 Th1 cells. The adoptive transfer of Yersinia-specific Th1 cells from PP and MLN conferred protection against a lethal orogastric inoculum with Y. enterocolitica as revealed by survival post-infection. However, determination of bacterial counts in infected organs revealed that the transfer of PP T cells conferred protection in spleen but not in MLN and PP, whereas the transfer of T cells from MLN reduced bacterial counts in both spleen and MLN but not in PP. To elucidate the different protection pattern we wanted to track the transferred cells in vivo. For this purpose the cells were labelled with the stable green fluorescent cell linker PKH2-GL prior to the adoptive transfer. In vivo tracking of these cells revealed that the distribution pattern of transferred T cells in spleen, MLN and PP correlated closely with the protection pattern observed after Yersinia infection. Thus, most cells were recovered from the spleen, while only few cells were recovered from MLN and PP. In keeping with these results a rapid and significant increase in interferon-gamma (IFN-gamma) production in the spleen of mice after adoptive transfer of T cell lines was observed. Taken together, the present results demonstrate that intestinal CD4 Th1 cells from PP and MLN may be involved in the defence against Y. enterocolitica at different sites of the infection, and that PKH2-GL labelling is a suitable tool to characterize T cell functions in vivo.
authors.affiliation: Max-von-Pettenkofer-Institut für Hygiene und Mikrobiologie der Ludwig Maximilians Universität München, Germany.
authors.forename: V A
authors.initials: VA
authors.lastname: Kempf
authors.forename: E
authors.initials: E
authors.lastname: Bohn
authors.forename: A
authors.initials: A
authors.lastname: Noll
authors.forename: C
authors.initials: C
authors.lastname: Bielfeldt
authors.forename: I B
authors.initials: IB
authors.lastname: Autenrieth
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D007371:Interferon-gamma / Q000096:biosynthesis; D007422:Intestines / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D015008:Yersinia enterocolitica / Q000276:immunology*
chemical_list: D007371:Interferon-gamma