Comparative FISH-mapping of the prion protein gene (PRNP) on cattle, river buffalo, sheep and goat chromosomes.
Comparative FISH-mapping of the prion protein gene (PRNP) was performed on cattle (BTA), river buffalo (BBU), sheep (OAR) and goat (CHI) chromosomes using a PCR-product as a probe and R-banding. PRNP was mapped to BTA13q17, BBU14q15, OAR13q15 and CHI13q15 according to standard nomenclatures. These chromosomes and bands were homoeologous among the four species, confirming the high degree of gene and chromosome banding conservation among bovids. Furthermore, the assignment of PRNP to river buffalo and goat chromosomes allowed us to indirectly assign the bovine syntenic group U11 to specific chromosomes, since it is the first in situ localization on BBU14 and CHI13.
Iannuzzi
L
L
National Research Council (CNR), I.A.B.B.A.M., Naples, Italy.
Palomba
R
R
Di Meo
G P
GP
Perucatti
A
A
Ferrara
L
L
D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002478:Cells, Cultured; D002874:Chromosome Mapping; D006041:Goats / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology; D016133:Polymerase Chain Reaction; D011328:Prions / Q000235:genetics*; D012756:Sheep / Q000235:genetics*
D011328:Prions