A novel phosphorylated lipid counteracts activation of the renal plasma membrane (Ca2+ + Mg2+)ATPase by endogenous phosphatidylinositol-4-phosphate.
The plasma membrane (Ca2+ + Mg2+)ATPase is activated by acidic phospholipids in reconstituted systems. In this report it is shown that reversible phosphorylation of endogenous phosphatidylinositol regulates the renal plasma membrane (Ca2+ + Mg2+)ATPase, and that a novel phosphorylated lipid that can be isolated from the same membrane strongly counteracts the stimulatory effect of phosphatidylinositol-4-phosphate.
Guilherme
A
A
Departmento de Bioquímica Médica, Universidad Federal do Rio de Janeiro, Brazil.
Reis-Silva
C
C
Moraes-Albuquerque
J H
JH
Oliveira
M M
MM
Vieyra
A
A
D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology*; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology
D008563:Membrane Lipids; D018129:Phosphatidylinositol Phosphates; C037178:phosphatidylinositol 4-phosphate; D012969:Sodium Fluoride; D017301:Ca(2+) Mg(2+)-ATPase