Glycobiology and proteomics: is mass spectrometry the Holy Grail?
One characteristic of glycoproteins is that they are separated by two-dimensional electrophoresis (2-D PAGE) into typical 'trains' of protein spots which separate on the basis of different isoelectric point (pI) and/or molecular mass. The pattern of these trains often varies in development and disease. While the isoforms differ both in the number of sites of glycosylation and the types of carbohydrate attached to the protein, classical methods of glycan analysis are insensitive at the levels typically separated by 2-D PAGE. Developments in mass spectrometry technologies have enabled the characterization of most of the oligosaccharide attributes to be determined on picomole amounts of protein. These techniques are beginning to allow the glycoform heterogeneity on 2-D separated glycoproteins to be analyzed.
Packer
N H
NH
Macquarie University Centre for Analytical Biotechnology, School of Biological Sciences, Macquarie University, Sydney, NSW, Australia. nicolle.packer@mq.edu.au
Harrison
M J
MJ
D000595:Amino Acid Sequence; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D011506:Proteins / Q000032:analysis*
D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D011506:Proteins