title: Involvement of selectin-like carbohydrate binding specificity in human gamete interaction.
abstract: The recognition of carbohydrate epitopes by complimentary protein receptors has been shown to be a critical factor in gamete interaction in many different animal species. In this study it was hypothesized that, in the human, gamete binding requires an interaction between selectin ligands on the zona pellucida and putative egg binding proteins on the sperm surface. The hemizona assay (a unique internally controlled bioassay that evaluates tight binding of sperm to the zona) and advanced methods of carbohydrate analysis were used to test this hypothesis. From these tests it was shown that oligosaccharide recognition is also required for initial human gamete binding. This study suggests the existence of distinct egg binding proteins on human sperm that can bind to selectin ligands. Additionally, the results suggest a possible convergence in the types of carbohydrate sequences recognized during initial human gamete binding and immune/inflammatory cell interactions. Glycoconjugates that manifest selectin-ligand activity and that express specific carbohydrate epitopes have potent contraceptive and immunosuppressive effects. Such specific oligosaccharide sequences may provide an appropriate recognition signal for embryo development and protection.
authors.affiliation: Jones Institute for Women's Health, Department of Obstetrics & Gynecology, Eastern Virginia Medical School, Norfolk, USA.
authors.forename: S
authors.initials: S
authors.lastname: Oehninger
authors.forename: M
authors.initials: M
authors.lastname: Patankar
authors.forename: M
authors.initials: M
authors.lastname: Seppala
authors.forename: G F
authors.initials: GF
authors.lastname: Clark
mesh_terms: D050260:Carbohydrate Metabolism; D005854:Germ Cells; D000071816:Glycodelin; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D008297:Male; D011257:Pregnancy Proteins / Q000378:metabolism; D019087:Selectins / Q000378:metabolism*; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D000071816:Glycodelin; D006023:Glycoproteins; C045246:PAEP protein, human; D011257:Pregnancy Proteins; D019087:Selectins