Glucocorticoids inhibit the bradykinin B2 receptor increase induced by interleukin-1beta in human bronchial smooth muscle cells.
We studied the effect of the glucocorticoids, dexamethasone and budesonide, on the interleukin-1beta-induced increase of bradykinin B2 receptors in cultured human bronchial smooth muscle cells, a cellular model of bronchial hyperreactivity. Both compounds prevented the increase of the bradykinin B2 mRNA and the bradykinin-induced inositol phosphate accumulation. These results demonstrate a direct effect of glucocorticoids on airway smooth muscle hyperresponsiveness mediated through inhibition of the over-expression of receptors for contractile mediators induced by inflammatory mediators.
Schmidlin
F
F
Laboratoire de Neuroimmunopharmacologie, Faculté de Pharmacie, INSERM U425, Université Louis Pasteur, Strasbourg I, Illkirch, France.
Scherrer
D
D
Landry
Y
Y
Gies
J P
JP
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D016535:Bronchial Hyperreactivity / Q000188:drug therapy / Q000378:metabolism; D001993:Bronchodilator Agents / Q000494:pharmacology*; D019819:Budesonide / Q000494:pharmacology*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004347:Drug Interactions; D006801:Humans; D007295:Inositol Phosphates / Q000096:biosynthesis; D007375:Interleukin-1 / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009130:Muscle, Smooth / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure*; D012333:RNA, Messenger / Q000378:metabolism; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin / Q000096:biosynthesis*
D000893:Anti-Inflammatory Agents; D001993:Bronchodilator Agents; D007295:Inositol Phosphates; D007375:Interleukin-1; D012333:RNA, Messenger; D043782:Receptor, Bradykinin B2; D018002:Receptors, Bradykinin; D019819:Budesonide; D003907:Dexamethasone