Regulation of pectic enzymes from the exo-1 mutant strain of Neurospora crassa: effects of glucose, galactose, and galacturonic acid.
The exo-1 mutant of Neurospora crassa produced and secreted pectolytic activities when incubated in the presence of pectin-containing biological materials. This study shows that polygalacturonase, pectate lyase and pectin lyase activities were induced in media supplemented with galactose or galacturonic acid, indicating that these sugars induced the synthesis of pectinases. Pectinesterase activity was undetectable. Polygalacturonase activity was better induced by galactose than by galacturonic acid. The reverse was true for lyase activities. The inducing effect of galactose and galacturonic acid seemed to be different: (i) a mixture of galactose and galacturonic acid synergistically increased the production of pectic enzymes, as compared to that in the presence of one of these sugars; (ii) the inducing effect of galacturonic acid was partially repressed by glucose; (iii) in contrast, the inducing effect of galactose, rather than repressed, was enhanced by the presence of glucose. Altogether, these data point out to a complex mechanism of regulation of pectolytic enzymes by pectin-containing organic substances.
Crotti
L B
LB
Departamento de Biologia, Faculdade de Filosofía, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Brazil.
Terenzi
H F
HF
Jorge
J A
JA
de Lourdes
M
M
Polizeli
M L
ML
D004790:Enzyme Induction / Q000502:physiology; D005656:Fungal Proteins / Q000096:biosynthesis; D005690:Galactose / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D006603:Hexuronic Acids / Q000494:pharmacology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D010368:Pectins / Q000378:metabolism*; D011096:Polygalacturonase / Q000096:biosynthesis*; D011133:Polysaccharide-Lyases / Q000096:biosynthesis / Q000378:metabolism
D005656:Fungal Proteins; D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases; C027349:pectin lyase; C023911:pectate lyase; D005947:Glucose; D005690:Galactose