title: Pyrene degradation by Mycobacterium sp. strain KR2.
abstract: A Mycobacterium sp., strain KR2 which was able to utilise pyrene as sole source of carbon and energy was isolated from a polycyclic aromatic hydrocarbon (PAH) contaminated soil originating from the area of a former gaswork plant. The isolate metabolised up to 60% of the pyrene added (0.5 mg/mL) within 8 days at 20 degrees C. Cis-4,5-pyrene dihydrodiol, 4,5-phenanthrene dicarboxylic acid, 1-hydroxy-2-naphthoic acid, 2-carboxybenzaldehyde, phthalic acid, and protocatechuic acid were identified as degradation products. Based on these findings a degradation pathway for pyrene is suggested which is in good accordance with the data published so far on bacterial pyrene metabolism.
authors.affiliation: GSF-National Research Centre for Environment and Health, Institute of Ecological Chemistry, Neuherberg, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Rehmann
authors.forename: H P
authors.initials: HP
authors.lastname: Noll
authors.forename: C E
authors.initials: CE
authors.lastname: Steinberg
authors.forename: A A
authors.initials: AA
authors.lastname: Kettrup
mesh_terms: D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009161:Mycobacterium / Q000737:chemistry / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011721:Pyrenes / Q000737:chemistry / Q000378:metabolism*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes