Genotoxicity and oxidative stress induced by pesticide exposure in bovine lymphocyte cultures in vitro.
The genotoxic activity of the pesticides gliphosate, vinclozolin and DPX-E9636 was studied in in vitro cultures of bovine lymphocytes, using chromosome aberration (CA) and sister chromatid exchange (SCE) frequencies as genetic end-points and a variation of glucose 6-phosphate dehydrogenase (G6PD) enzyme activity as a marker of changes in the normal cell redox state. Results indicated a statistically significant increase of structural aberrations, sister chromatid exchanges and G6PD activity, suggesting that the pesticides tested induce either oxidative stress or a mutagenic effect in this species. The evaluation of both mitotic index and cell viability, after pesticide exposure, demonstrates a high cytotoxic effect which is always associated with the observed genotoxic effect.
Lioi
M B
MB
Dipartimento di Scienze delle Produzioni Animali, Università della Basilicata, Potenza, Italy.
Scarfì
M R
MR
Santoro
A
A
Barbieri
R
R
Zeni
O
O
Di Berardino
D
D
Ursini
M V
MV
D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005998:Glycine / Q000031:analogs & derivatives / Q000633:toxicity; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010080:Oxazoles / Q000633:toxicity; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010575:Pesticides / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects; D014508:Urea / Q000031:analogs & derivatives / Q000633:toxicity; D000097797:Glyphosate
D005954:Glucosephosphate Dehydrogenase; D005998:Glycine; D009153:Mutagens; D010080:Oxazoles; D010575:Pesticides; D014508:Urea; D000097797:Glyphosate; C113968:DPX E9636; C025643:vinclozolin