title: Aneurysm induced by periarterial application of elastase heals spontaneously.
abstract: BACKGROUND
  The pathogenesis of aortic aneurysms remains largely unknown, despite aneurysmal rupture being an increasingly common catastrophe.
  
  
  METHODS
  This study was designed to elucidate the mechanism of arterial dilatation histologically and electron microscopically, utilising a new animal model. Elastase, 3.0 mg/ml, was applied to the abdominal aortae of 18 New Zealand white rabbits from the adventitia side for 3 hours. The rabbits were sacrificed at 0, 3, 14, 28, 42 and 90 days after the procedure (n=4, 3, 2, 2, 2, 3).
  
  
  RESULTS
  Two rabbits were found to have developed aortic rupture. On day 0, elastase application induced fusiform aneurysms up to 1.62+/-0.14 times the pre-elastase application aortic diameter. Dilated walls revealed medial elastolysis, degeneration of smooth muscle cells (SMCs) and damaged endothelial cells. By day 3, the smooth muscle cells had changed to the synthetic type. Aneurysms did not progress, and after 42 days, showed gradual shrinkage. By day 90, aortic diameters had nearly normalised.
  
  
  CONCLUSIONS
  Aortic walls also returned to the pre-elastase application thickness and some mature medial elastic lamellae showed regeneration. Medial smooth muscle cells reverted to the contractile type. Aortic dilatation induced by peri-aortic application of elastase heals spontaneously, accompanied by regeneration of smooth muscle cells. Irreversible degeneration of medial smooth muscle cells appears to be more critical to aneurysm formation than degeneration of elastic lamellae.
authors.affiliation: First Department of Surgery, Faculty of Medicine, The University of Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Origuchi
authors.forename: H
authors.initials: H
authors.lastname: Shigematsu
authors.forename: N
authors.initials: N
authors.lastname: Izumiyama
authors.forename: K
authors.initials: K
authors.lastname: Nakamura
authors.forename: A
authors.initials: A
authors.lastname: Toku
authors.forename: T
authors.initials: T
authors.lastname: Muto
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects / Q000648:ultrastructure; D017544:Aortic Aneurysm, Abdominal / Q000209:etiology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D009131:Muscle, Smooth, Vascular / Q000648:ultrastructure; D010196:Pancreatic Elastase; D011817:Rabbits
chemical_list: D010196:Pancreatic Elastase