Characterization of psoralen-oleic acid cycloadducts and their possible involvement in membrane photodamage.
By UVA irradiation of an ethanol solution of psoralen and oleic acid, four main photoproducts have been isolated and characterized: two have cis,cis structure; the other two are trans,cis. The same adducts have been isolated from the photoreaction of psoralen with beta-oleoyl-gamma-stearoyl-1-alpha-phosphatidylcholine followed by enzymatic hydrolysis with phospholipase A2. The four isomers stimulate protein kinase C to almost the same extent.
Frank
S
S
Department of Pharmaceutical Sciences, University of Padua, Italy.
Caffieri
S
S
Raffaelli
A
A
Vedaldi
D
D
Dall'Acqua
F
F
D001792:Blood Platelets / Q000201:enzymology; D005363:Ficusin / Q000528:radiation effects*; D006801:Humans; D007536:Isomerism; D008968:Molecular Conformation; D015394:Molecular Structure; D019301:Oleic Acid / Q000528:radiation effects*; D010741:Phospholipases A / Q000378:metabolism; D054467:Phospholipases A2; D011493:Protein Kinase C / Q000097:blood*; D013237:Stereoisomerism; D014466:Ultraviolet Rays
D019301:Oleic Acid; D011493:Protein Kinase C; D010741:Phospholipases A; D054467:Phospholipases A2; D005363:Ficusin