HIV load in highly purified CD8+ T cells retrieved from pulmonary and blood compartments.
The pulmonary microenvironment can be HIV infected from the asymptomatic period of HIV infection and it has been demonstrated that HIV presence elicits a discrete virus-specific CTL immune response in the lungs. In this study we analyzed T cell subsets and compared the proportion of CD8+ T cells harboring HIV in the lung and in the circulation of HIV-seropositive patients with T cell alveolitis. Proviral load was assessed using the DNA-polymerase chain reaction technique on highly purified CD8+ T cells isolated from peripheral blood and bronchoalveolar lavage. The proviral load of pulmonary CD8+ T cells showed an upward trend with respect to corresponding samples isolated from the peripheral blood of the same patients.
Semenzato
G
G
Department of Clinical and Experimental Medicine, Padua University School of Medicine, Italy.
Agostini
C
C
Chieco-Bianchi
L
L
De Rossi
A
A
D000328:Adult; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000821:virology; D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D018414:CD8-Positive T-Lymphocytes / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000097:blood*; D006679:HIV Seropositivity / Q000097:blood / Q000821:virology; D006801:Humans; D008168:Lung / Q000166:cytology / Q000821:virology*; D016676:Macrophages, Alveolar / Q000821:virology; D008297:Male; D016133:Polymerase Chain Reaction; D019562:Viral Load
D004279:DNA, Viral