Expression of galectin-1 in the mouse olfactory system.
Primary sensory olfactory axons arise from the olfactory neuroepithelium that lines the nasal cavity and then project via the olfactory nerve into the olfactory bulb. The beta-galactoside binding lectin, galectin-1, and its laminin ligand have been implicated in the growth of these axons along this pathway. In galectin-1 null mutant mice, a subpopulation of primary sensory olfactory axons fails to reach its targets in the olfactory bulb. In the present study we examined the spatiotemporal expression pattern of galectin-1 in normal mice in order to understand its role in the development of the olfactory nerve pathway. At E15.5, when olfactory axons have already contacted the olfactory bulb, galectin-1 was expressed in the cartilage and mesenchyme surrounding the nasal cavity but was absent from the olfactory neuroepithelium, nerve and bulb. Between E16.5 and birth galectin-1 began to be expressed by olfactory nerve ensheathing cells in the lamina propria of the neuroepithelium and nerve fibre layer. Galectin-1 was neither expressed by primary sensory neurons in the olfactory neuroepithelium nor by their axons in the olfactory nerve. Laminin, a galectin-1 ligand, also exhibited a similar expression pattern in the embryonic olfactory nerve pathway. Our results reveal that galectin-1 is dynamically expressed by glial elements within the nerve fibre layer during a discrete period in the developing olfactory nerve pathway. Previous studies have reported galectin-1 acts as a substrate adhesion molecule by cross-linking primary sensory olfactory neurons to laminin. Thus, the coordinate expression of galectin-1 and laminin in the embryonic nerve fibre layer suggests that these molecules support the adhesion and fasciculation of axons en route to their glomerular targets.
Tenne-Brown
J
J
Department of Anatomy and Cell Biology, University of Melbourne, Parkville, Vic., Australia.
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D000818:Animals; D001369:Axons / Q000737:chemistry; D002356:Cartilage / Q000737:chemistry / Q000196:embryology; D004848:Epithelium / Q000737:chemistry / Q000196:embryology; D037483:Galectin 1; D006388:Hemagglutinins / Q000032:analysis*; D007797:Laminin / Q000032:analysis; D008297:Male; D008648:Mesoderm / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D009296:Nasal Cavity / Q000737:chemistry / Q000196:embryology; D009832:Olfactory Nerve / Q000737:chemistry / Q000196:embryology*; D009833:Olfactory Pathways / Q000737:chemistry* / Q000196:embryology*; D009928:Organ Specificity
D037483:Galectin 1; D006388:Hemagglutinins; D007797:Laminin