title: Histopathological and morphometric study of the late effects of heavy-ion irradiation on the spinal cord of the rat.
abstract: The late effects of heavy-ion irradiation on the spinal cord of the rat were investigated histologically and morphometrically. After a single exposure of each animal's lower thoracic and lumbar spinal cord to a carbon-ion beam, the animals were observed clinically for up to 69 weeks and their spinal cords were examined histologically after sacrifice. Paralysis of the hind limbs appeared from 16 to 20 weeks after irradiation with 20 Gy or more. The first histological change seen was vacuolization in the marginal white matter, which appeared 19 to 25 weeks after irradiation with more than 10 Gy. After irradiation with more than 15 Gy, bilateral destructive cavities occurred in the white matter, especially in the lateral tract. These histological changes were similar to those reported frequently for X irradiation. The mean cross-sectional area of the blood vessels in the irradiated spinal cord increased in a manner that was dependent on dose and was significantly larger 15 to 17 weeks after irradiation with 30 Gy. Reconstruction of small destructive lesions from serial sections consistently revealed dilated veins in the centers of these lesions. The effective dose that induces 50% incidence of hind-limb paralysis and destructive cavity formation (ED50) as determined using a curve-fitting method was 18.5 and 19.5 Gy, respectively, and the latent period was shorter than that for X irradiation.
authors.affiliation: Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: R
authors.initials: R
authors.lastname: Okeda
authors.forename: S
authors.initials: S
authors.lastname: Matsushita
authors.forename: A
authors.initials: A
authors.lastname: Kawano
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000473:pathology / Q000528:radiation effects; D002244:Carbon; D004307:Dose-Response Relationship, Radiation; D006614:Hindlimb; D008297:Male; D009186:Myelin Sheath / Q000473:pathology / Q000528:radiation effects; D010243:Paralysis / Q000209:etiology; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D013047:Specific Pathogen-Free Organisms; D013116:Spinal Cord / Q000098:blood supply / Q000473:pathology / Q000528:radiation effects*; D013118:Spinal Cord Diseases / Q000473:pathology*; D013997:Time Factors; D014617:Vacuoles
chemical_list: D002244:Carbon