Cloning of a human ether-a-go-go potassium channel expressed in myoblasts at the onset of fusion.
An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K+ current, I(K(NI)), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K+ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32-41, and is expressed as a approximately 9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I(K(NI)) indicating that h-eag constitutes the channel responsible for this current in vivo.
Occhiodoro
T
T
Département de Physiologie, Centre Médical Universitaire, Hôpital Cantonal Universitaire, Switzerland.
Bernheim
L
L
Liu
J H
JH
Bijlenga
P
P
Sinnreich
M
M
Bader
C R
CR
Fischer-Lougheed
J
J
D000293:Adolescent; D000328:Adult; D000595:Amino Acid Sequence; D001921:Brain / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003001:Cloning, Molecular; D051638:Ether-A-Go-Go Potassium Channels; D006801:Humans; D007223:Infant; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D015221:Potassium Channels / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D017421:Sequence Analysis
D051638:Ether-A-Go-Go Potassium Channels; C496884:KCNH1 protein, human; D015221:Potassium Channels