Allosamidin inhibits the fragmentation of Acremonium chrysogenum but does not influence the cephalosporin-C production of the fungus.
The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble intracellular chitinase activity was resistant to the inhibitory effect of allosamidin. On the other hand, about 76% of the total chitinase activity localised in both the soluble and insoluble enzyme fractions was effectively inhibited by allosamidin. All the chitinase activities were measured using a new procedure based on purified A. chrysogenum chitin as substrate.
Sándor
E
E
Department of Microbiology and Biotechnology, Kossuth Lajos University, Debrecen, Hungary.
Pusztahelyi
T
T
Karaffa
L
L
Karányi
Z
Z
Pócsi
I
I
Biró
S
S
Szentirmai
A
A
Pócsi
I
I
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000164:Acremonium / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D002511:Cephalosporins / Q000096:biosynthesis*; D002688:Chitinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D014312:Trisaccharides / Q000494:pharmacology*
D002511:Cephalosporins; D004791:Enzyme Inhibitors; D014312:Trisaccharides; C054851:allosamidin; C025163:cephalosporin C; D002688:Chitinases; D000117:Acetylglucosamine