Chemiluminescent determination of esterases in monocytes.
Esterase from monocytes promotes the hydrolysis of 2-methyl-1-propenylbenzoate (MPB) yielding 2-methyl-1-propenol, which is oxidized by horseradish peroxidase/H2O2 producing triplet acetone. The chemiluminescence of this reaction can be enhanced by the addition of 9,10-dibromoanthracene-2-sulphonate. The non-specific esterase present in monocytes is responsible for MPB hydrolysis, since (a) the chemiluminescence of the reaction was inhibited by fluoride, and (b) cells that do not contain a significant amount of non-specific esterases, e.g. lymphocytes and neutrophils, did not trigger light emission. The analytical application of this reaction is considered.
da Fonseca
L M
LM
Departamento de Análises Clínicas e Toxicológicas da Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Brazil.
Yavo
B
B
Catalani
L H
LH
Falcão
R P
RP
Brunetti
I L
IL
Campa
A
A
D001565:Benzoates; D004950:Esterases / Q000097:blood*; D005456:Fluorescent Dyes; D006098:Granulocytes / Q000201:enzymology*; D006735:Horseradish Peroxidase; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007700:Kinetics; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology*
C101261:2-methyl-1-propenylbenzoate; D001565:Benzoates; D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; D004950:Esterases