Endocytosis by the corneal endothelium. I. Regulation of binding and transport of hemeproteins and peroxidase-conjugated lectins across the tissue.
Binding, internalization, and movement of hemeproteins and peroxidase-conjugated lectins across organ cultured rat corneal endothelia has been investigated. Horseradish peroxidase (HRP) type II, bound to the surface, was minimally internalized and was easily washed off. In contrast, HRP-VI bound and was rapidly internalized. Reaction product was observed in vesicles, endosomes, multivesicular bodies, and extended along the length of the intercellular space (ICS) to Descemet's membrane. Studies at 4 degrees C indicated HRP-VI bound uniformly along the surface in a punctate fashion. Exposure to polylysine or mannose significantly decreased uptake. Other tracers such as HRP-VIII, -IX, catalase, and microperoxidase exhibited limited uptake by the tissue. However, endothelia vigorously internalized soybean agglutinin (SBA)-HRP, and reaction product was found intracellularly and within the ICS at the cell/Descemet's membrane interface. Internalization and the appearance of SBA-HRP within the ICS was diminished following polylysine or mannose treatment. Experiments at 4 degrees C indicated that SBA-HRP binding and uptake were temperature sensitive. Wheat germ agglutinin (WGA)-HRP was also strongly endocytosed and reaction product was visualized within vesicles, endosomes, and multivesicular bodies. Although WGA-HRP reaction product was observed within the ICS, none was detected at the level of Descemet's membrane. The WGA competitive sugar N-acetyl-D-glucosamine, reduced endocytosis, whereas exposure to unlabeled WGA and mannose together reduced uptake. These results indicate endothelia exhibit differential uptake of various hemeproteins and lectins which is dependent on charge, mannose receptors, and appropriate surface sugars.
Gordon
S R
SR
Department of Biological Sciences, Oakland University, Rochester, MI 48309-4476, USA. srgordon@Oakland.edu
Czerwinski-Mowers
D
D
Marchand
J
J
Shuffett
R
R
D000818:Animals; D001692:Biological Transport; D004705:Endocytosis; D004728:Endothelium, Corneal / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006420:Hemeproteins / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism; D037102:Lectins / Q000378:metabolism*; D009924:Organ Culture Techniques; D037121:Plant Lectins; D011107:Polylysine / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D030262:Soybean Proteins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate / Q000378:metabolism; D010835:Phytohemagglutinins
D006420:Hemeproteins; D006735:Horseradish Peroxidase; D007527:Isoenzymes; D037102:Lectins; D037121:Plant Lectins; D011107:Polylysine; D030262:Soybean Proteins; D018998:Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate; C028824:soybean lectin; D010835:Phytohemagglutinins