Genetic damage induced by methylglyoxal and methylglyoxal plus X-rays in Drosophila melanogaster germinal cells.
The effect of methylglyoxal (MG) and MG administered prior to X-irradiation was investigated in Drosophila melanogaster germinal cells using the sex-linked recessive lethal (s.l.r.l.), II-III autosomal translocation (AT) and X-chromosome nondisjunction (ND) tests. For the s.l.r.l. test the males were either injected with MG (0.5 M, 0.75 M or 1.7 M) or fed for 24 h (1 M) and two 24 h broods (A and B) were obtained. For the AT test the males were injected with MG 1.7 M and the same brooding scheme was followed. ND was tested in females fed on MG 1 M. The only effect observed after MG treatment was a significant increase on the yield of s.l.r.l. with MG 1.7 M. In the combined treatments MG was administered prior to irradiation with 20 Gy of X-rays and the induction of s.l.r.l. and AT was assessed. Pre-treatment with MG 0.75 M and 1.7 M enhanced the frequency of s.l.r.l. in cells sampled in brood B, consisting mainly of the rather hypoxic late spermatids. It is suggested that this radiosensitizing effect could be ascribed to a decrease in the level of glutathione due to the metabolization of MG.
Barnett
B M
BM
Radiobiología-Comisión Nacional de Energía Atómica, Av. Libertador 8250, 1429, Buenos Aires, Argentina.
Muñoz
E R
ER
D000818:Animals; D004247:DNA / Q000528:radiation effects*; D004249:DNA Damage; D004331:Drosophila melanogaster / Q000235:genetics* / Q000528:radiation effects; D005260:Female; D005804:Genes, Lethal; D005854:Germ Cells; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D009630:Nondisjunction, Genetic; D011765:Pyruvaldehyde / Q000633:toxicity*; D014178:Translocation, Genetic
D009153:Mutagens; D011765:Pyruvaldehyde; D004247:DNA