Characterization of mouse fibroblast (NIH3T3) and fibrosarcoma cell lines (WEHI-164 and MFS 8) using PMRS.
Proton magnetic resonance Spectroscopy (PMRS) has been used to study the differences between immortalized fibroblasts and fibrosarcoma cells of different grade. One and two dimensional purged correlation spectroscopy (PCOSY) have been used to assess intact viable fibroblast and fibrosarcoma cells, and differences in the triglyceride, cellular metabolite, and cell surface fucosylation patterns between the three cell lines have been observed. The clinical implication of this study is the potential use of PMRS as an adjunct to conventional histopathology.
Jayashree
B
B
Biophysics Division, Cancer Institute, Madras.
Visalakshi
V
V
Rajalakshmi
K R
KR
Sukumaran
M S
MS
Moni
M S
MS
Rajkumar
T
T
Deshmukh
S
S
D016475:3T3 Cells / Q000166:cytology / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002453:Cell Cycle; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D005354:Fibrosarcoma / Q000378:metabolism* / Q000473:pathology*; D005643:Fucose / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D051379:Mice; D010743:Phospholipids / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D010743:Phospholipids; D014280:Triglycerides; D005643:Fucose