title: Mutation of cis-proline 207 in mitochondrial creatine kinase to alanine leads to increased acid stability.
abstract: We show that the mutation of an uncharged residue far from the active site to another uncharged residue can have effects on the active site without disturbing the overall structure of the protein. Cis-proline 207 of mitochondrial creatine kinase was mutated to alanine. The mutant showed a decrease in the pH-optimum for ATP synthesis by 1.5 units while the maximum relative activity was lowered to 53% of the wild-type enzyme. In the direction of ATP consumption, the pH optimum was lowered by 1.3 units and the maximum relative activity was 49% of the wild-type enzyme. The enzyme kinetic parameters Km and Kd for the substrates did not change dramatically, indicating a largely unperturbed active site. Small-angle X-ray scattering was used to investigate the structural change concomitant with the mutation, yielding a scattering profile only slightly different from that of the wild-type enzyme. Neither the radius of gyration nor the molecular mass showed any significant differences, leading to the conclusion that quarternary organization and fold of the mutant and the wild-type enzymes were similar. Theoretical analysis suggests the most probable primary source of structural change to be a transition of residue 207 peptide bond torsional angle co from the cis to the trans configuration.
authors.affiliation: Institute of Cell Biology, Zürich, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Forstner
authors.forename: A
authors.initials: A
authors.lastname: Müller
authors.forename: D
authors.initials: D
authors.lastname: Rognan
authors.forename: M
authors.initials: M
authors.lastname: Kriechbaum
authors.forename: T
authors.initials: T
authors.lastname: Wallimann
mesh_terms: D003402:Creatine Kinase / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000648:ultrastructure; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D011392:Proline / Q000235:genetics; D011487:Protein Conformation; D014961:X-Ray Diffraction
chemical_list: D011392:Proline; D003402:Creatine Kinase