Intracranial androgenic activation of male-typical behaviours in house mice: concurrent stimulation of the medial preoptic area and medial nucleus of the amygdala.
This experiment examined whether testosterone proprionate (T) action in the medial preoptic area (MPO) would synergize with T action in the medial nucleus of the amygdala (AME) for the expression of androgen-dependent behaviors in house mice. Cannulae containing T were bilaterally implanted into the MPO, the AME, or both areas concurrently (MPO/AME) of castrated males. In addition, other castrates were implanted subcutaneously with empty Silastic capsules (BSIL) or Silastic capsules containing T (TSIL). All subjects were examined for the following androgen-dependent, male-typical behaviors: mounting, urinary scent marking, preference for female urine over male urine, preference for female over male conspecifics and ultrasonic mating vocalizations. MPO implants restored ultrasonic vocalizations and preference for females, but had little or no effect upon urine marking, mounting or preference for female urine. In contrast, AME implants were ineffective at restoring any of these male-typical behaviors. The combined MPO/AME implants were not more effective in restoring male-typical behaviors than MPO implants alone, thus providing no evidence for synergy in hormone action between these two brain areas. In general, castration (BSIL) resulted in low levels of all behaviors whereas systemic T replacement (TSIL) resulted in high levels of behavior, verifying the androgen-dependence of these behaviors. Group differences in male-typical behavior could not be accounted for by differences in general activity levels. Moreover, none of the brain-implanted groups had larger seminal vesicles than those of the BSIL. Thus, when the brain implants affected behavior, they most probably did so through their effects within the brain. Although the AME is a target for steroid hormones and is an important area for the expression of male-typical behaviors, intracranial T implants into the AME did not demonstrate a role for androgen in the AME in restoring male-typical behaviors in castrated mice.
Sipos
M L
ML
Department of Biological Sciences, Lehigh University, Bethlehem, PA 18015, USA.
Nyby
J G
JG
D000679:Amygdala / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D004343:Drug Implants; D005260:Female; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D009812:Odorants; D009919:Orchiectomy; D009929:Organ Size; D011301:Preoptic Area / Q000187:drug effects* / Q000502:physiology; D012669:Seminal Vesicles / Q000033:anatomy & histology; D012727:Sex Characteristics; D012726:Sexual Behavior, Animal / Q000187:drug effects; D013739:Testosterone / Q000008:administration & dosage / Q000494:pharmacology*; D014556:Urine; D014828:Vocalization, Animal
D004343:Drug Implants; D013739:Testosterone