[Pattern of various cytokeratins of normal vulva, vulvar intraepithelial neoplasia (VIN) and vulvar carcinoma].
The presence of specific keratins can be of diagnostic value for studying normal and neoplastic epithelium of the vulva. The aim of the present study was to investigate normal, preneoplastic and neoplastic epithelium of the vulva. Keratins 5, 6 and 18, identified by a polyspecific anti-human CK antibody (clone LP 34, DAKO), and the keratin subtypes 7, 10, 14, 18, 19 and 20 of normal, dysplastic and malignant vulval epithelium (paraffin-embedded sections) were detected by immunohistochemical APAAP staining. Keratins 5, 6, and 18 (clone LP 34) and keratin subtype 10 are expressed in the upper third of the normal vulval epithelium. In mild and moderate intraepithelial neoplasia only a few cells express these keratins. In patients with severe intraepithelial neoplasia (VIN III) the expression of these keratins seems to be associated with recurrence of the disease. In biopsy specimens of patients without recurrence we find positive results for keratins 5, 6 and 18 (clone LP 34) and keratin 10. If patients have a recurrence of the disease, expression of these keratins is only diffuse or is absent. The expression of these keratin subtypes in vulval carcinomas is mostly seen in differentiated cells. There was no association between recurrence and keratin pattern. We have not found any other expression of the tested keratin subtypes in VIN and in vulval carcinoma.
Küppers
V
V
Frauenklinik der Heinrich-Heine-Universität, Düsseldorf.
Koldovsky
U
U
Somville
T
T
Bender
H G
HG
D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D002278:Carcinoma in Situ / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D004848:Epithelium / Q000473:pathology; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007633:Keratins / Q000032:analysis*; D009364:Neoplasm Recurrence, Local / Q000473:pathology; D011230:Precancerous Conditions / Q000473:pathology*; D011379:Prognosis; D014844:Vulva / Q000473:pathology; D014846:Vulvar Neoplasms / Q000473:pathology*
D014408:Biomarkers, Tumor; D007633:Keratins