Mapping an endometrial cancer tumor suppressor gene at 10q25 and development of a bacterial clone contig for the consensus deletion interval.
Frequent loss of chromosome 10q sequences in endometrial cancers suggests the involvement of a tumor suppressor gene. Previous loss-of-heterozygosity (LOH)studies have pointed to the 10q25-q26 region as the likely site of a tumor suppressor involved in endometrial tumorigenesis (S. L. Peiffer et al., 1995, Cancer Res. 55: 1922-1926; S. Nagase et al., 1996, Br. J. Cancer 74: 1979-1983; S. Nagase et al.,1997, Cancer Res. 57: 1630-1633). In an attempt to define further the localization of a tumor suppressor gene at 10q25, we screened a panel of 123 endometrioid adenocarcinomas for loss of heterozygosity of 10q25.3 sequences. Forty-three (35%) revealed LOH at one or more loci. The observed patterns of allelic loss define a minimum consensus region of deletion between D10S221 and D10S610. A sequence-ready bacterial clone contig and a long-range restriction map for a 1-Mb interval spanning the deletion region were developed as the first step in experiments directed toward the discovery the 10q25 tumor suppressor.
Peiffer-Schneider
S
S
Department of Surgery, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, Missouri, 63110, USA.
Noonan
F C
FC
Mutch
D G
DG
Simpkins
S B
SB
Herzog
T
T
Rader
J
J
Elbendary
A
A
Gersell
D J
DJ
Call
K
K
Goodfellow
P J
PJ
D018269:Carcinoma, Endometrioid / Q000235:genetics*; D002874:Chromosome Mapping / Q000379:methods; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D003001:Cloning, Molecular; D005260:Female; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D015183:Restriction Mapping; D017384:Sequence Deletion / Q000235:genetics
D005819:Genetic Markers