Alterations in the enzyme activity and protein contents of protein disulfide isomerase in rat tissues during fasting and refeeding.
Protein disulfide isomerase (PDI) is an enzyme that participates in the formation of disulfide bonds. It is also known to be the subunits of some enzymes and the membrane-associated thyroid hormone-binding protein. In this study, we measured the quantitative distribution of PDI protein in rat tissues and examined the relationship between protein level and enzyme activity in PDI during fasting and refeeding. Western blotting with specific anti-PDI antiserum detected the PDI protein band of 55 kd. Among several tissues, liver contained the largest amount of PDI protein, followed by kidney and fat, in which one-third to one-fourth of the hepatic PDI protein existed. The PDI protein band was also detected in heart and muscle. Fasting for 3 days decreased PDI protein levels in rat liver by 40%; control levels were recovered after 3 days of refeeding. The same change was observed in kidney. PDI activity, measured by the scrambled ribonuclease method, did not show the parallel alteration to PDI protein level in liver and kidney. Isomerase activity decreased to 50% of control values during fasting, but did not recover by refeeding. Thyroidal status did not affect either PDI protein level or isomerase activity. These findings show that fasting and refeeding affect PDI protein and enzyme activity, and that PDI protein level does not always reflect PDI activity.
Mikami
T
T
Department of Internal Medicine, Hamamatsu University School of Medicine, Shizuoka, Japan.
Genma
R
R
Nishiyama
K
K
Ando
S
S
Kitahara
A
A
Natsume
H
H
Yoshimi
T
T
Horiuchi
R
R
Nakamura
H
H
D000818:Animals; D015153:Blotting, Western; D005215:Fasting; D008099:Liver / Q000201:enzymology; D008297:Male; D019704:Protein Disulfide-Isomerases / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013963:Thyroid Hormones / Q000032:analysis
D013963:Thyroid Hormones; D019704:Protein Disulfide-Isomerases