title: [The identification of minimal residual disease in the bone marrow and peripheral blood in neuroblastoma. The prognostic and therapeutic implications].
abstract: A highly sensitive and specific methodology to detect neuroblastoma cells in the bone marrow and peripheral blood of children with neuroblastoma is of critical importance for proper staging and treatment of these patients. In addition, patients with bone marrow infiltration at diagnosis need to undergo regular investigation to measure the effectiveness of chemotherapy (so called "in vivo" purging). Finally, the evaluation of autologous stem cells taken from bone marrow or peripheral blood is necessary to rule out or minimise the possibility of reinfusing tumor cells to the patient following myeloablative therapy. The authors provide a "state of the art" data on this complicated issue and give their preliminary results of their own experience, mainly concerning the immunocytological methods.
authors.affiliation: Sezione di Onco-ematologia, Azienda Ospedale Meyer, Università di Firenze, Italia.
authors.forename: L B
authors.initials: LB
authors.lastname: Faulkner
authors.forename: A
authors.initials: A
authors.lastname: Tamburini
authors.forename: V
authors.initials: V
authors.lastname: Tintori
authors.forename: A
authors.initials: A
authors.lastname: Paoli
authors.forename: A
authors.initials: A
authors.lastname: Tondo
authors.forename: G
authors.initials: G
authors.lastname: Bernini
authors.forename: D
authors.initials: D
authors.lastname: Medicina
authors.forename: M
authors.initials: M
authors.lastname: Brisigotti
authors.forename: M V
authors.initials: MV
authors.lastname: Corrias
authors.forename: P
authors.initials: P
authors.lastname: Scaruffi
authors.forename: C
authors.initials: C
authors.lastname: Rosanda
authors.forename: M S
authors.initials: MS
authors.lastname: Lo Piccolo
authors.forename: E
authors.initials: E
authors.lastname: Viscardi
authors.forename: C
authors.initials: C
authors.lastname: Milanaccio
authors.forename: A
authors.initials: A
authors.lastname: Garaventa
authors.forename: B
authors.initials: B
authors.lastname: De Bernardi
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D019046:Bone Marrow Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000556:secondary* / Q000628:therapy; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D018365:Neoplasm, Residual; D009360:Neoplastic Cells, Circulating / Q000378:metabolism / Q000473:pathology*; D009423:Nervous System Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology* / Q000556:secondary* / Q000628:therapy; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor