title: Retinoic acid increases in the retina of the chick with form deprivation myopia.
abstract: We previously reported that expression of retinoic acid receptor beta increases in the sclera of the 2-week-old chick with form deprivation myopia (FDM) and that all-trans-retinoic acid (t-RA) influences proliferation and differentiation of scleral cells. The purpose of this study was to quantify t-RA in the retina of the chick with FDM and to investigate the role of t-RA in FDM in the chick. FDM was induced in 2-day-old chicks by placement of a translucent plastic goggle over one eye, with the contralateral eye used as a control. After 5 days, the chicks were sacrificed. t-RA was extracted from neural retina and served for high-performance liquid chromatography analysis. 3H-t-RA was used for normalization. Pieces of the retinae from 5 eyes served as one sample. As a result, t-RA was 09.60 +/- 0.86 ng/eye (0.387 +/- 0.056 ng/mg protein) in the myopic retina was significantly higher than that in the control (p < 0.05, n = 7). These results demonstrate that t-RA increases in the retina within 5 days after visual deprivation. This finding suggests that t-RA may play a role in the metabolic changes in FDM.
authors.affiliation: Department of Ophthalmology, School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Seko
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
authors.forename: T
authors.initials: T
authors.lastname: Tokoro
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D008297:Male; D009216:Myopia / Q000209:etiology* / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012683:Sensory Deprivation / Q000502:physiology*; D014212:Tretinoin / Q000378:metabolism*; D015349:Vision, Monocular / Q000502:physiology*
chemical_list: D014212:Tretinoin