Effects of beta-ionone on the expression of cytochrome P450s and NADPH-cytochrome P450 reductase in Sprague Dawley rats.
We have recently reported that beta-ionone induces cytochrome P450 (P450) 2B1 in rats. Effects of beta-ionone on the expression of other P450 isozymes and NADPH-P450 reductase were further investigated in Sprague Dawley rats. Administration of beta-ionone subcutaneously 72 and 48 h before sacrificing the animals not only significantly induced the liver microsomal activities of P450-associated enzymes and NADPH-P450 reductase, but also clearly increased in the level of P450 1A1/2, P450 2C, and NADPH-P450 reductase proteins. The induction of P450 1A1/2 and 2C by beta-ionone was much greater in male than in female as measured by western immunoblotting. Reverse transcriptase-polymerase chain reactions showed that, in addition to P450 2B1 and 2B2 mRNAs, P450 1A2, 2C6 and NADPH-P450 reductase mRNAs were increased when beta-ionone was administered. Our previous and present results indicated that beta-ionone may induce several P450s and NADPH-P450 reductase by the accumulation of their corresponding mRNAs.
Jeong
T C
TC
Toxicology Research Center, Korea Research Institute of Chemical Technology, Yusung, Taejon, South Korea.
Gu
H K
HK
Chun
Y J
YJ
Yun
C H
CH
Han
S S
SS
Roh
J K
JK
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D019388:Cytochrome P-450 CYP1A2 / Q000096:biosynthesis; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D000072467:Cytochrome P450 Family 2; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D007527:Isoenzymes / Q000096:biosynthesis*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009251:NADPH-Ferrihemoprotein Reductase / Q000096:biosynthesis*; D045792:Norisoprenoids; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012737:Sex Factors; D013255:Steroid 21-Hydroxylase / Q000096:biosynthesis; D013250:Steroid Hydroxylases / Q000096:biosynthesis; D013729:Terpenes / Q000494:pharmacology*
D007527:Isoenzymes; D045792:Norisoprenoids; D012333:RNA, Messenger; D013729:Terpenes; D003577:Cytochrome P-450 Enzyme System; C008157:beta-ionone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C089877:Cyp2c6v1 protein, rat; D019388:Cytochrome P-450 CYP1A2; D019362:Cytochrome P-450 CYP2B1; D000072467:Cytochrome P450 Family 2; C025619:steroid 16-beta-hydroxylase; D013255:Steroid 21-Hydroxylase; D009251:NADPH-Ferrihemoprotein Reductase