title: Effect of acarbose (alpha-glucosidase inhibitor) on disaccharase activity in small intestine in KK-Ay and ddY mice.
abstract: The hypoglycemic effect and the alpha-glucosidase activity inhibition of acarbose (AC:alpha-glucosidase inhibitor) were investigated in normal and KK-Ay mice, an animal model of noninsulin-dependent diabetes mellitus (NIDDM). AC improved hyperglycemia after an oral administration of maltose or sucrose, dose dependently in normal mice (1, 10, and 50mg/kg body weight) and in KK-Ay mice (50mg/kg). Furthermore, AC (50mg/kg) significantly inhibited maltase and sucrase activities in the small intestines of normal and KK-Ay mice (inhibitory efficacy: sucrase > maltase). The enzymatic inhibition in KK-Ay mice is stronger than in normal mice. However, AC (50 mg/kg) did not suppress the blood glucose in oral lactose tolerance and did not inhibit the lactase activity in either normal or KK-Ay mice. These findings indicate that the AC effect on the inhibition of alpha-glucosidase activity is selective for sucrase and maltase in normal and NIDDM mice.
authors.affiliation: Suzuka University of Medical Science and Technology, Mie, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Miura
authors.forename: T
authors.initials: T
authors.lastname: Koide
authors.forename: R
authors.initials: R
authors.lastname: Ohichi
authors.forename: M
authors.initials: M
authors.lastname: Kako
authors.forename: M
authors.initials: M
authors.lastname: Usami
authors.forename: E
authors.initials: E
authors.lastname: Ishihara
authors.forename: N
authors.initials: N
authors.lastname: Yasuda
authors.forename: H
authors.initials: H
authors.lastname: Ishida
authors.forename: Y
authors.initials: Y
authors.lastname: Seino
authors.forename: K
authors.initials: K
authors.lastname: Tanigawa
mesh_terms: D020909:Acarbose; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology*; D004186:Disaccharidases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D007785:Lactose; D008297:Male; D008320:Maltose; D051379:Mice; D008817:Mice, Mutant Strains; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013395:Sucrose; D014312:Trisaccharides / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D014312:Trisaccharides; D013395:Sucrose; D008320:Maltose; D004186:Disaccharidases; D000520:alpha-Glucosidases; D013393:Sucrase; D007785:Lactose; D020909:Acarbose