Duodenal infusions of palmitic, stearic or oleic acids differently affect mammary gland metabolism of fatty acids in lactating dairy cows.
The effect of dietary lipids on the fatty acid (FA) profile of cows' milk fat is mainly dependent on digestive processes and mammary gland uptake and metabolism of FA. The objective of this study was to determine the separate effects of high arterial concentrations of 16:0, 18:0 and cis-18:1(n-9) on uptake, synthesis and 18:0 desaturation rate in the mammary gland of lactating dairy cows, via arterio-venous differences and mammary gland balance of FA. In a 4 x 4 Latin square, four lactating Holstein cows with cannula in the proximal duodenum were infused duodenally with a mixture providing daily 0 (C treatment) or 500 g FA with mainly 16:0 (P treatment), 18:0 (S treatment) or cis-18:1(n-9) (O treatment). Significantly higher arterial concentrations of infused FA in arterial plasma nonesterified FA and triglycerides (NETGFA) were observed with P and O treatments, but the effect of the S treatment was much lower. Arterio-venous differences of NETGFA increased with arterial concentrations. The number of synthesized FA in the mammary gland was not significantly affected by duodenal infusion of FA. Mean chain length was significantly reduced by P and O treatments, suggesting an effect of mammary gland uptake of long-chain FA on the termination process of mammary gland synthesis of FA. Across all treatments, 4:0 mammary gland balance increased linearly (r = 0.67, P = 0.004) with mammary gland FA uptake. Mammary gland desaturation of 18:0 to cis-18:1(n-9) averaged 52% and was not significantly affected by treatments, but was reduced by trans-18:1 mammary gland uptake. Uptake, synthesis and desaturation of FA by the mammary gland of dairy cows are affected by arterial concentrations of 16:0, 18:0 and cis-18:1(n-9).
Enjalbert
F
F
Ecole Nationale Vétérinaire, Département Elevage & Produits, Laboratoire d'Alimentation, 31076 Toulouse Cedex, France.
Nicot
M C
MC
Bayourthe
C
C
Moncoulon
R
R
D000818:Animals; D002417:Cattle; D004032:Diet; D004386:Duodenum; D005227:Fatty Acids / Q000008:administration & dosage* / Q000096:biosynthesis / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000098:blood supply / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D019308:Palmitic Acid / Q000008:administration & dosage*; D013229:Stearic Acids / Q000008:administration & dosage* / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D013229:Stearic Acids; D014280:Triglycerides; D019308:Palmitic Acid; C031183:stearic acid