Prostaglandin-independent stimulation of interleukin-6 production by fibrinogen degradation product D in perfused murine liver.
Bacterial endotoxin (LPS) and fibrinogen degradation product D (FDP-D) are both potent stimulators of interleukin-6 (IL-6) production in liver, however, there are differences in their metabolic effects. The aim of the present study was to compare the role of prostaglandins in the enhancement of IL-6 production by LPS or FDP-D in perfused mouse livers. Indomethacin inhibited the effect of LPS significantly but was ineffective in the case of FDP-D. Accordingly, production of prostaglandins D2 and E2 was not elevated following the addition of FDP-D, while their formation was increased several fold by LPS. At the same time interleukin-1 (IL-1) production in perfused liver rose markedly upon the addition of FDP-D. It is suggested that prostaglandins are not involved in the effects of FDP-D on the liver. The stimulatory effect of FDP-P on IL-6 production might be the consequence of elevated IL-1 levels.
Csala
M
M
Department of Medical Chemistry, Semmelweis University of Medicine, Budapest, Hungary.
Léránt
I
I
Bánhegyi
G
G
Kardon
T
T
Puskás
F
F
Mucha
I
I
Machovich
R
R
Falus
A
A
Mandl
J
J
D000818:Animals; D015232:Dinoprostone / Q000096:biosynthesis; D005338:Fibrin Fibrinogen Degradation Products / Q000494:pharmacology*; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051379:Mice; D010477:Perfusion; D015230:Prostaglandin D2 / Q000096:biosynthesis; D011453:Prostaglandins / Q000494:pharmacology*
D005338:Fibrin Fibrinogen Degradation Products; D007375:Interleukin-1; D015850:Interleukin-6; D011453:Prostaglandins; D015232:Dinoprostone; D015230:Prostaglandin D2; D007213:Indomethacin