Surface plasmon resonance analysis at a supported lipid monolayer.
Methods for the formation of supported lipid monolayers on top of a hydrophobic self assembled monolayer in a surface plasmon resonance instrument are described. Small unilamellar vesicles absorb spontaneously to the surface of the hydrophobic self-assembled monolayer to form a surface which resembles the surface of a cellular membrane. Lipophilic ligands, such as small acylated peptides or glycosylphosphatidylinositol-anchored proteins, were inserted into the absorbed lipid and binding of analytes to these ligands was analysed by surface plasmon resonance. Conditions for the formation of lipid monolayers have been optimised with respect to lipid type, chemical and buffer compatibility, ligand stability and reproducibility.
Cooper
M A
MA
Department of Chemistry, Cambridge Centre for Molecular Recognition, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. mc221@cam.ac.uk
Try
A C
AC
Carroll
J
J
Ellar
D J
DJ
Williams
D H
DH
D000900:Anti-Bacterial Agents / Q000378:metabolism; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000378:metabolism; D001427:Bacterial Toxins; D004731:Endotoxins / Q000378:metabolism; D006020:Glycopeptides; D017261:Glycosylphosphatidylinositols / Q000737:chemistry / Q000378:metabolism; D006460:Hemolysin Proteins; D050356:Lipid Metabolism; D008055:Lipids / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*
D000900:Anti-Bacterial Agents; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004731:Endotoxins; D006020:Glycopeptides; D017261:Glycosylphosphatidylinositols; D006460:Hemolysin Proteins; D008055:Lipids; C030956:insecticidal crystal protein, Bacillus Thuringiensis