title: 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide as a fluorescent labelling reagent for determination of carboxylic acids in precolumn high-performance liquid chromatography.
abstract: A fluorescent labelling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide, was designed for the determination of carboxylic acids by precolumn HPLC. The reagent was reacted with carboxylic acids at 100 degrees C for 15 min in the presence of an activating reagent (a mixture of triphenylphosphine, 2,2'-dipyridyldisulfide and pyridine) and produced highly fluorescent derivatives, which were separated on a reversed-phase column by fluorescence measurement at 317 nm (excitation) and 380 nm (emission). The detection limits were 4-12 fmol/injection. The reagent was used for HPLC assays of long chain fatty acids in human serum by isocratic elution.
authors.affiliation: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: M
authors.initials: M
authors.lastname: Ikeno
authors.forename: Y
authors.initials: Y
authors.lastname: Ishii
authors.forename: Y
authors.initials: Y
authors.lastname: Tsuruta
mesh_terms: D002264:Carboxylic Acids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000097:blood; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000494:pharmacology; D006801:Humans; D010797:Phthalimides / Q000138:chemical synthesis* / Q000494:pharmacology; D012997:Solvents; D013050:Spectrometry, Fluorescence
chemical_list: C093940:4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl chloride; D002264:Carboxylic Acids; D005227:Fatty Acids; D005456:Fluorescent Dyes; D010797:Phthalimides; D012997:Solvents