Expression of steroidogenic acute regulatory protein messenger ribonucleic acid is limited to theca of healthy bovine follicles collected during recruitment, selection, and dominance of follicles of the first follicular wave.
Expression of mRNA encoding steroidogenic acute regulatory protein (StAR) in bovine follicles during recruitment and selection was examined. Dairy heifers (4-5/time period) were ovariectomized at 12, 24, 36, 48, 60, 72, 84, or 96 h after initiation of the first follicular wave (Time 0) after estrus. Follicles were collected and stored at -80 degrees C until sectioning. Expression of StAR mRNA was localized by in situ hybridization and quantified by image analysis. Expression of StAR mRNA was first detected in theca interna of antral follicles as small as 0.5 mm in diameter and increased with increasing follicular size (>/= 4 mm; r = 0.75; p < 0.001). StAR mRNA was undetectable in granulosa of healthy follicles at any size or stage of follicular wave examined. However, granulosa or luteinized granulosa of some advanced or late atretic follicles expressed StAR mRNA. During recruitment, StAR mRNA expression in theca cells was similar among recruited follicles (4-8 mm). During selection of dominant follicles (36-48 h), StAR mRNA was expressed in theca of more than one follicle (7-9 mm); therefore, expression of StAR mRNA may not be associated with dominant follicle selection. StAR mRNA in theca was higher (p < 0.05) at 48 h after initiation of the first follicular wave than at 12, 24, and 36 h, and it remained elevated thereafter through 96 h. Dominant follicles expressed more (p < 0.01) StAR mRNA in theca than did subordinate healthy follicles. Healthy follicles expressed higher (p < 0.05) StAR mRNA in theca than atretic follicles. In summary, levels of StAR mRNA increased in theca with stage of follicular wave and size of follicles. Follicular atresia was associated with reduced expression of StAR mRNA in theca cells. The results indicate that expression of StAR mRNA in theca may not be the primary limiting factor for follicular recruitment and selection.
Bao
B
B
Departments of Animal Sciences, University of Missouri, Columbia, Missouri 65211, USA.
Calder
M D
MD
Xie
S
S
Smith
M F
MF
Salfen
B E
BE
Youngquist
R S
RS
Garverick
H A
HA
D000818:Animals; D015152:Blotting, Northern; D002417:Cattle; D005260:Female; D005498:Follicular Phase / Q000502:physiology*; D007091:Image Processing, Computer-Assisted; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D013799:Theca Cells / Q000378:metabolism*; D000098682:Steroidogenic Acute Regulatory Protein
D008565:Membrane Proteins; D010750:Phosphoproteins; D015347:RNA Probes; D012333:RNA, Messenger; D000098682:Steroidogenic Acute Regulatory Protein