title: [Absence of HHV-8 virus detected in immature hemangiomas in infants].
abstract: BACKGROUND
  The aim of our study was to search for the presence of HHV-8 DNA sequences in Biopsy specimens from hemangioma of the infancy.
  
  
  METHODS
  The study included 9 biopsies from hemangioma. DNA of human beta-globin gene and HHV-8 were searched for by PCR using specific primers. Amplified products were revealed after an hybridization with an internal probe digoxigenin-labelled.
  
  
  RESULTS
  Human beta-globin gene could be detected in all samples illustrating the absence of PCR inhibitor. HHV-8 could never be detected in samples analyzed.
  
  
  CONCLUSIONS
  Our study does not imply any causative role of HHV-8 in the pathogenesis of hemangioma. This result must be confirmed by serologic studies.
authors.affiliation: Service de Dermato-Vénéréologie, Hôpital Tarnier-Cochin, Paris.
authors.forename: N
authors.initials: N
authors.lastname: Dupin
authors.forename: O
authors.initials: O
authors.lastname: Enjolras
authors.forename: M
authors.initials: M
authors.lastname: Wassef
authors.forename: I
authors.initials: I
authors.lastname: Gorin
authors.forename: M
authors.initials: M
authors.lastname: Gradadam
authors.forename: J M
authors.initials: JM
authors.lastname: Huraux
authors.forename: H
authors.initials: H
authors.lastname: Agut
authors.forename: J P
authors.initials: JP
authors.lastname: Escande
mesh_terms: D001706:Biopsy; D002675:Child, Preschool; D004279:DNA, Viral / Q000032:analysis*; D005914:Globins / Q000235:genetics; D006391:Hemangioma / Q000473:pathology / Q000821:virology*; D006566:Herpesviridae Infections / Q000150:complications* / Q000473:pathology; D019288:Herpesvirus 8, Human / Q000235:genetics*; D006801:Humans; D007223:Infant; D016133:Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral; D005914:Globins