Genetic analysis of Histoplasma capsulatum strains isolated from clinical specimens in thailand by a PCR-based random amplified polymorphic DNA method.
Thirteen strains of Histoplasma capsulatum were isolated from clinical specimens, including those from AIDS patients, in Thailand. Random amplified polymorphic DNA (RAPD) analysis with three different PCR primers showed that the DNA fingerprint patterns of the Thai isolates were very similar to each other and homogeneous, with only one exceptional strain, although the patterns were clearly different from those of a reference North American strain with all primers tested. Although the difference in the DNA fingerprinting patterns was minor, Thai isolates could be classified into two to four groups. A common PCR band (about 700 bp) in the patterns of all H. capsulatum strains was extracted, and its DNA sequence was determined. A new PCR primer set for the identification of H. capsulatum species was developed based on this sequence information. This primer set was 100% successful in the identification of the reference strain as well as all Thai isolates. The results of specificity tests of the primer set for the identification of the fungus are also discussed.
Poonwan
N
N
Department of Medical Sciences, National Institute of Health, Nonthaburi, Thailand.
Imai
T
T
Mekha
N
N
Yazawa
K
K
Mikami
Y
Y
Ando
A
A
Nagata
Y
Y
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000382:microbiology*; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics; D006658:Histoplasma / Q000235:genetics* / Q000302:isolation & purification; D006660:Histoplasmosis / Q000175:diagnosis / Q000382:microbiology*; D006801:Humans; D008969:Molecular Sequence Data; D009656:North America; D019105:Random Amplified Polymorphic DNA Technique; D013785:Thailand
D017931:DNA Primers; D004271:DNA, Fungal