title: Interstitial and large chromosome 1p deletion occurs in localized and disseminated neuroblastomas and predicts an unfavourable outcome.
abstract: We studied chromosome 1p loss of heterozygosity (1p-LOH) in 53 neuroblastomas (NBs) using 15 (CA)n repeat loci, which covered a region of 90 cM. We also assessed chromosome 1p36 deletion by fluorescence in situ hybridization (FISH) on interphase nuclei. 1p-LOH was found in 19 (36%, 95% confidence interval (CI) 23-50%) NBs. We detected interstitial and large deletion in both localized and disseminated tumours and in one tumour of a patient at stage 4S. Allelic loss was frequently observed in 1p36 and 1p32 regions. In patients older than 1 year of age (53 versus 13%, P < 0.002) we detected significant chromosome 1p deletion and it was associated with MYCN amplification (P = 0.001). Overall survival (OS) analysis showed that 1p-LOH is predictive of a poor outcome (odds ratio 16.5, 95% CI 5.4-50.9%); therefore, 1p-LOH should be regarded as an additional tumour progression marker in neuroblastoma.
authors.affiliation: Department of Biomedicine of Evolutive Age, University of Bari, Genova, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Iolascon
authors.forename: C
authors.initials: C
authors.lastname: Lo Cunsolo
authors.forename: L
authors.initials: L
authors.lastname: Giordani
authors.forename: R
authors.initials: R
authors.lastname: Cusano
authors.forename: K
authors.initials: K
authors.lastname: Mazzocco
authors.forename: M
authors.initials: M
authors.lastname: Boumgartner
authors.forename: P
authors.initials: P
authors.lastname: Ghisellini
authors.forename: M F
authors.initials: MF
authors.lastname: Faienza
authors.forename: L
authors.initials: L
authors.lastname: Boni
authors.forename: B
authors.initials: B
authors.lastname: De Bernardi
authors.forename: M
authors.initials: M
authors.lastname: Conte
authors.forename: G
authors.initials: G
authors.lastname: Romeo
authors.forename: G P
authors.initials: GP
authors.lastname: Tonini
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D005784:Gene Amplification; D017353:Gene Deletion; D016259:Genes, myc / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D019656:Loss of Heterozygosity; D009447:Neuroblastoma / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D016019:Survival Analysis