Mode of action of linenscin OC2 against Listeria innocua.
Linenscin OC2 is a small hydrophobic substance produced by the orange cheese coryneform bacterium Brevibacterium linens OC2. Linenscin OC2 inhibits growth of gram-negative bacteria with an altered outer membrane permeability and gram-positive bacteria. It is also able to lyse eucaryotic cells. The mode of action of linenscin OC2 on the Listeria innocua cytoplasmic membrane and the effects of environmental parameters were investigated. Addition of low doses of linenscin OC2 resulted in an immediate perturbation of the permeability properties of the cytoplasmic membrane and of the bacterial energetic state. Linenscin OC2 induced a loss of cytoplasmic potassium, depolarization of the cytoplasmic membrane, complete hydrolysis of internal ATP, efflux of inorganic phosphate, and transient increase in oxygen consumption. Potassium loss occurred in the absence of a proton motive force and was severely reduced at low temperatures, presumably as a result of increased ordering of the lipid hydrocarbon chains of the cytoplasmic membrane. We propose that linenscin OC2 interacts with the cytoplasmic membrane and that the permeability changes observed at low doses reflect the formation of pore-like structures in this membrane.
Boucabeille
C
C
Institut de Génétique et Microbiologie, Unité de Recherche Associée au Centre National de la Recherche Scientifique 2225, Université Paris-Sud, 91405 Orsay Cedex 05, France.
Letellier
L
L
Simonet
J M
JM
Henckes
G
G
D000255:Adenosine Triphosphate / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000494:pharmacology*; D001951:Brevibacterium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003470:Culture Media; D008087:Listeria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D008564:Membrane Potentials; D008826:Microbial Sensitivity Tests; D010101:Oxygen Consumption / Q000187:drug effects; D010710:Phosphates / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D013696:Temperature
D000900:Anti-Bacterial Agents; D003470:Culture Media; D010710:Phosphates; D000255:Adenosine Triphosphate; D011188:Potassium