title: Influence of osmotic stress on heat shock proteins 25 and 72 in mouse mesangial cells.
abstract: Previous studies have shown intense staining for heat shock protein 25 (HSP25) in the extraglomerular mesangium (EGM). Because relationships are believed to exist between osmotic stress, expression of HSP25, and protection against stress and because the EGM may be exposed to high local tonicity, we examined the expression of HSP25 and the major stress-inducible and cytoprotective HSP72 in mouse mesangial cells and embryonic lung fibroblasts (3T3) after exposure to hypertonic stress (addition of 150 mM NaCl to the medium for two to seven days). Mesangial, but not 3T3, cells expressed high levels of HSP25 already under control conditions, whereas neither cell line contained HSP72. Hypertonic treatment neither enhanced (mesangial cells) or induced (3T3 cells) HSP25 expression. HSP72, however, was induced strongly in 3T3 cells, but only minimally in mesangial cells. The high level of HSP25 in mesangial cells thus seems not to be a consequence of high tonicity in the EGM because cultured mesangial cells express HSP25 already under control conditions, and osmotic stress did not induce HSP25 in either cell line. Furthermore, high amounts of HSP25 seem to reduce the requirement for HSP72 after stress exposure, suggesting that, in mesangial cells, HSP25 might assume some functions of HSP72.
authors.affiliation: Department of Physiology, University of Munich, Germany. eva.mueller@physiol.med.uni-muenchen.de
authors.forename: E
authors.initials: E
authors.lastname: Müller
authors.forename: A
authors.initials: A
authors.lastname: Burger-Kentischer
authors.forename: W
authors.initials: W
authors.lastname: Neuhofer
authors.forename: A
authors.initials: A
authors.lastname: Schober
authors.forename: F X
authors.initials: FX
authors.lastname: Beck
mesh_terms: D016475:3T3 Cells / Q000166:cytology / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology; D005920:Glomerular Mesangium / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000235:genetics*; D006982:Hypertonic Solutions / Q000494:pharmacology; D008168:Lung / Q000166:cytology; D051379:Mice; D018832:Molecular Chaperones; D009363:Neoplasm Proteins / Q000235:genetics*; D015345:Oligonucleotide Probes; D009995:Osmosis; D012333:RNA, Messenger / Q000032:analysis; D013314:Stress, Mechanical
chemical_list: D050884:HSP72 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486113:Hsbp1 protein, mouse; D006982:Hypertonic Solutions; D018832:Molecular Chaperones; D009363:Neoplasm Proteins; D015345:Oligonucleotide Probes; D012333:RNA, Messenger