A cell cycle regulated MAP kinase with a possible role in cytokinesis in tobacco cells.
Mitogen-activated protein (MAP) kinases have been demonstrated to have a role in meiosis but their involvement in mitotic events is less clear. Using a peptide antibody raised against the tobacco MAP kinase p43(Ntf6) and extracts from synchronized tobacco cell suspension cultures, we show that this kinase is activated specifically during mitosis. Entry into mitosis appears to be necessary for the activation of the kinase, which occurs as a post-translational event. The activation of the kinase occurs in late anaphase/early telophase. The p43(Ntf6) protein shows a transient localization to the cell plate in anaphase cells, in the middle of the two microtubule arrays characteristic of the phragmoplast, a plant-specific structure involved in laying down the new cell wall. The combined data support a role for the MAP kinase p43(Ntf6) in cytokinesis in tobacco cells.
Calderini
O
O
Institute of Microbiology and Genetics, University of Vienna, Dr Bohrgasse 9, A-1030 Vienna, Austria.
Bögre
L
L
Vicente
O
O
Binarova
P
P
Heberle-Bors
E
E
Wilson
C
C
D000705:Anaphase; D001549:Benzamides / Q000494:pharmacology; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002460:Cell Line; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D005656:Fungal Proteins / Q000378:metabolism; D015151:Immunoblotting; D008677:Metaphase; D008870:Microtubules / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases; D008938:Mitosis; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D010940:Plant Proteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D010947:Plants, Toxic; D011494:Protein Kinases / Q000378:metabolism; D011687:Purines / Q000494:pharmacology; D000077546:Roscovitine; D029702:Schizosaccharomyces pombe Proteins; D013692:Telophase; D014026:Nicotiana / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology
D001549:Benzamides; D018797:Cell Cycle Proteins; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D009578:Nitrobenzenes; D009946:Organothiophosphorus Compounds; D010940:Plant Proteins; D011687:Purines; D029702:Schizosaccharomyces pombe Proteins; C051655:Suc1 protein, S pombe; D000077546:Roscovitine; C001059:pronamide; C017823:amiprophos methyl; D011494:Protein Kinases; C102333:histone H1 kinase; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D018844:Cyclin-Dependent Kinases; D020928:Mitogen-Activated Protein Kinases; C084275:NTF3 protein, Nicotiana tabacum; D017239:Paclitaxel