Comparative susceptibility of two forms of Anopheles sinensis Wiedemann 1828 (Diptera : Culicidae) to infection with Plasmodium falciparum, P. vivax, P. yoelii and the determination of misleading factor for sporozoite identification.
Two karyotypic forms of laboratory-raised Anopheles sinensis, ie Form A (XY1) and Form B (XY2), were experimentally infected with various indigenous strains of Plasmodium falciparum and P. vivax using an artificial membrane feeding technique, and a rodent malaria, P. yoelii, using a direct feeding technic and dissected 7-9 days and 10-15 days after feeding for oocyst and sporozoite rates, respectively. The results revealed that two forms of An. sinensis were refractory vectors for P. falciparum and P. yoelii since 0% of oocyst and sporozoite rates were obtained, but poor vectors for P. vivax since 0.00-85.71% and 0.00-5.88% of oocyst and sporozoite rates were recovered. The sporozoite-like crystal found in the median lobe of the salivary gland of An. sinensis which could be a misleading factor in identification of true sporozoites in the salivary glands is reported for the first time.
Rongsriyam
Y
Y
Department of Medical Entomology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Jitpakdi
A
A
Choochote
W
W
Somboon
P
P
Tookyang
B
B
Suwonkerd
W
W
D000818:Animals; D000852:Anopheles / Q000469:parasitology*; D008018:Life Cycle Stages; D010963:Plasmodium falciparum / Q000254:growth & development / Q000502:physiology*; D010966:Plasmodium vivax / Q000254:growth & development / Q000502:physiology*; D010967:Plasmodium yoelii / Q000254:growth & development / Q000502:physiology*