title: p53 exon 7 mutations as a predictor of poor prognosis in patients with colorectal cancer.
abstract: We have studied 61 resected colorectal adenocarcinomas in order to investigate p53 mutations as a prognostic factor for this pathology. Mutations in exons 5-9 of the p53 gene were analyzed by the polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique followed by sequencing. Our data indicate that p53 exon 7 mutations were prevalent in the latest stages of colorectal carcinogenesis and patients bearing this alteration had the worst prognosis. Therefore, according to our results, mutations affecting exon 7 of the p53 gene could be considered as a useful marker of biological aggressiveness for colorectal cancer.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Iniesta
authors.forename: F J
authors.initials: FJ
authors.lastname: Vega
authors.forename: T
authors.initials: T
authors.lastname: Caldés
authors.forename: M
authors.initials: M
authors.lastname: Massa
authors.forename: C
authors.initials: C
authors.lastname: de Juan
authors.forename: F J
authors.initials: FJ
authors.lastname: Cerdán
authors.forename: A
authors.initials: A
authors.lastname: Sánchez
authors.forename: J A
authors.initials: JA
authors.lastname: López
authors.forename: A J
authors.initials: AJ
authors.lastname: Torres
authors.forename: J L
authors.initials: JL
authors.lastname: Balibrea
authors.forename: M
authors.initials: M
authors.lastname: Benito
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D005091:Exons / Q000235:genetics*; D005260:Female; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D011379:Prognosis; D016016:Proportional Hazards Models; D016019:Survival Analysis