Role of nitric oxide and thromboxane in the maintenance of cerebrovascular tone.
This study investigated the role of thromboxane A2 (TXA2) and neuronal nitric oxide (NO) synthase (nNOS)-derived NO in the maintenance of resting cerebrovascular tone. Rat basilar artery (BA) segments were mounted in myographs to study their isometric tension development. 7-Nitro indazole monosodium salt (7-NINA), a specific inhibitor of nNOS, had no significant effect on the resting tone, whereas the general NOS blocker N(G)-nitro-L-arginine (L-NA) induced strong contraction. The thromboxane (TP) receptor antagonist ICI 192605 induced weak vasodilation, and this effect was significantly enhanced after precontraction of the vessels with uridine-5'-triphosphate (UTP). Incubation of BA segments with ICI 192605 attenuated the contractile effect of UTP. These data indicate that nNOS is not involved in resting cerebrovascular NO production and that basal TXA2 release induces a weak contractile tone in the rat BA. Activation of P2U receptors by UTP appears to stimulate TXA2 release in these vessels.
Benyó
Z
Z
Department of Physiology, Ludwig-Maximilians University, Munich, Germany. u7232ah@sunmailhost.lrz-muenchen.de
Görlach
C
C
Wahl
M
M
D000818:Animals; D001488:Basilar Artery / Q000502:physiology; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology*; D004146:Dioxanes / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007191:Indazoles / Q000494:pharmacology; D008297:Male; D009569:Nitric Oxide / Q000502:physiology*; D009924:Organ Culture Techniques; D051381:Rats; D017208:Rats, Wistar; D013928:Thromboxane A2 / Q000502:physiology*; D014544:Uridine Triphosphate / Q000494:pharmacology; D014661:Vasoconstriction / Q000502:physiology
D004146:Dioxanes; D004791:Enzyme Inhibitors; D007191:Indazoles; C057498:ICI 192605; D009569:Nitric Oxide; D013928:Thromboxane A2; D014544:Uridine Triphosphate; C080122:7-nitroindazole