Effect of acarbose (alpha-glucosidase inhibitor) on disaccharase activity in small intestine in KK-Ay and ddY mice.
The hypoglycemic effect and the alpha-glucosidase activity inhibition of acarbose (AC:alpha-glucosidase inhibitor) were investigated in normal and KK-Ay mice, an animal model of noninsulin-dependent diabetes mellitus (NIDDM). AC improved hyperglycemia after an oral administration of maltose or sucrose, dose dependently in normal mice (1, 10, and 50mg/kg body weight) and in KK-Ay mice (50mg/kg). Furthermore, AC (50mg/kg) significantly inhibited maltase and sucrase activities in the small intestines of normal and KK-Ay mice (inhibitory efficacy: sucrase > maltase). The enzymatic inhibition in KK-Ay mice is stronger than in normal mice. However, AC (50 mg/kg) did not suppress the blood glucose in oral lactose tolerance and did not inhibit the lactase activity in either normal or KK-Ay mice. These findings indicate that the AC effect on the inhibition of alpha-glucosidase activity is selective for sucrase and maltase in normal and NIDDM mice.
Miura
T
T
Suzuka University of Medical Science and Technology, Mie, Japan.
Koide
T
T
Ohichi
R
R
Kako
M
M
Usami
M
M
Ishihara
E
E
Yasuda
N
N
Ishida
H
H
Seino
Y
Y
Tanigawa
K
K
D020909:Acarbose; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology*; D004186:Disaccharidases / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D007785:Lactose; D008297:Male; D008320:Maltose; D051379:Mice; D008817:Mice, Mutant Strains; D013393:Sucrase / Q000037:antagonists & inhibitors / Q000378:metabolism; D013395:Sucrose; D014312:Trisaccharides / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism
D001786:Blood Glucose; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D014312:Trisaccharides; D013395:Sucrose; D008320:Maltose; D004186:Disaccharidases; D000520:alpha-Glucosidases; D013393:Sucrase; D007785:Lactose; D020909:Acarbose