Glycated plasma proteins in experimentally induced acute toxic renal failure by dichromate injection: evidence for loss with urine and decreased plasma levels.
In the rat, a single subcutaneous injection of sodium dichromate (20 mg/kg) causes acute renal injury and significant polyuria, proteinuria, and glycosuria (peaking 2-3 days after treatment, and returning to normal by day 5) without any changes in the plasma levels of protein, glucose, and glycated haemoglobin. Surprisingly, the percentage levels of glycated plasma total proteins and albumin (assayed by boronate affinity chromatography) transiently and significantly decrease during recovery from proteinuria (days 4 and 10 after treatment) and were found in the normal range of values by day 18. These changes are concomitant with a significant increase in the percentage level of glycated albumin in urine. Constancy of total plasma protein and the temporal pattern of levels of glycation suggest that changes in the percentage values of glycated proteins are secondary to a transient selective loss of glycated plasma proteins in urine.
De Tata
V
V
Istituto di Patologia Generale, Medical School, University of Pisa, Italy.
Bombara
M
M
Novelli
M
M
Pingitore
R
R
Bergamini
E
E
D058186:Acute Kidney Injury / Q000097:blood* / Q000139:chemically induced / Q000652:urine; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism*; D002840:Chromates; D004591:Electrophoresis, Polyacrylamide Gel; D006442:Glycated Hemoglobin / Q000378:metabolism; D006031:Glycosylation; D008297:Male; D011507:Proteinuria / Q000097:blood* / Q000139:chemically induced; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism
D001786:Blood Glucose; D001798:Blood Proteins; D002840:Chromates; D006442:Glycated Hemoglobin; D012709:Serum Albumin; C016104:sodium bichromate