Expression of cartilage-specific functional matrix chondromodulin-I mRNA in rabbit growth plate chondrocytes and its responsiveness to growth stimuli in vitro.
Cartilage-specific functional matrix chondromodulin-I (ChM-I) is a 25 kDa glycoprotein purified from fetal bovine epiphyseal cartilage which stimulates the growth of rabbit chondrocytes and the colony formation of the cells in agarose. In the present study, we isolated rabbit ChM-I precursor CDNA by reverse transcription-polymerase chain reactions. Northern blot analysis revealed that the expression of ChM-I mRNA occurred in a tissue-specific manner in cartilage. Moreover, the ChM-I mRNA level was markedly changed in response to growth and differentiation stimuli in primary cultured chondrocytes. Among others, fibroblast growth factor-2, transforming growth factor-beta, and parathyroid hormone related peptide each markedly down-regulated the expression of ChM-I mRNA in chondrocytes. These results indicated that the expression ChM-I was placed under the dynamic control of local growth and differentiation factors.
Shukunami
C
C
Department of Molecular Interaction and Tissue Engineering, Kyoto University, Japan. shukunam@dent.osaka-u.ac.jp
Hiraki
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006132:Growth Plate / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D006133:Growth Substances / Q000235:genetics*; D006801:Humans; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011817:Rabbits; D019105:Random Amplified Polymorphic DNA Technique; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D017931:DNA Primers; D018076:DNA, Complementary; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; C068675:CNMD protein, human