Peptide substrates dissolved in dimethylformamide may be modified at the epsilon-amino group of lysyl residues, causing erroneous kinetic characterization of proteolytic enzymes.
Olesen
K
K
Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen Valby, DK-2500, Denmark. kol@crc.dk
Breddam
K
K
D002268:Carboxypeptidases / Q000378:metabolism; D043402:Cathepsin A; D004126:Dimethylformamide / Q000494:pharmacology*; D010450:Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000737:chemistry; D010455:Peptides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013379:Substrate Specificity
D010455:Peptides; D004126:Dimethylformamide; D002268:Carboxypeptidases; D010450:Endopeptidases; D043402:Cathepsin A; D008239:Lysine