title: Modification by ageing of the tetrodotoxin-sensitive sodium channels in rat skeletal muscle fibres.
abstract: Ageing leads to an impairment of muscle performance that may result from alteration of sarcolemma excitability. Therefore, we compare sodium channels of native fast-twitch skeletal muscle fibres of 21-26-month-old aged rats and 4-6-month-old young-adult rats, using the patch-clamp method. Extrajunctional sarcolemma of aged-rat fibres presented a higher sodium current density than that of young-rat fibres, which resulted from the presence of a higher number of available channels per membrane area. Open probability and availability voltage-dependence of sodium channels were similar in aged- and young-rat fibres, but permeation property was altered during ageing: aged-rat muscles showed a bimodal distribution of fibres with two values of sodium-channel conductance measured between -40 and 0 mV; a young phenotype with a conductance close to 18 pS overlapping that found in young-rat fibres and an aged phenotype with a lower approximately half conductance. Current-voltage curves extended to -60 and +20 mV showed that the aged-phenotype conductance level resulted from an outward rectification occurring in these aged-rat fibres. Furthermore, in these aged-rat fibres belonging to the aged phenotype, ensemble average sodium currents showed slower activation and inactivation kinetics. Sodium currents of the two phenotypes were blocked by 100 nM tetrodotoxin, therefore excluding possible denervation effect. These age-related modifications in sodium current may contribute to the alteration of muscle excitability and function observed during the ageing process.
authors.affiliation: Unità di Farmacologia, Dipartimento Farmaco-Biologico, Facoltà di Farmacia, Università degli Studi di Bari, via Orabona 4, I-70125 Bari, Italy.
authors.forename: J F
authors.initials: JF
authors.lastname: Desaphy
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.forename: P
authors.initials: P
authors.lastname: Imbrici
authors.forename: D
authors.initials: D
authors.lastname: Conte Camerino
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D008564:Membrane Potentials / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000502:physiology; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D015222:Sodium Channels / Q000187:drug effects*; D013779:Tetrodotoxin / Q000494:pharmacology*
chemical_list: D015222:Sodium Channels; D013779:Tetrodotoxin