Thioredoxin peroxidase (natural killer enhancing factor) regulation of activator protein-1 function in endothelial cells.
Thioredoxin peroxidase-1 (TxP-1), originally cloned as natural killer enhancing factor-B, belongs to a highly conserved antioxidant family. Tumor necrosis factor-alpha (TNF) activates the expression of activator protein-1 (AP-1) responsive genes. We show here that over-expression of TxP-1 blocks TNF-induced AP-1 activation in endothelial ECV304 cells, which was demonstrated by three independent experimental protocols: electromobility shift assay with AP-1 oligonucleotide probe; reporter gene expression with AP-1 binding site, and interleukin-8 production, which is dependent on AP-1. These results are consistent with the role of TxP-1 as an antioxidant and the previous reports that TNF-induced reactive oxygen species were responsible for AP-1 activation.
Shau
H
H
Division of Surgical Oncology, UCLA School of Medicine 90095, USA. hshau@ucla.edu
Huang
A C
AC
Faris
M
M
Nazarian
R
R
de Vellis
J
J
Chen
W
W
D000975:Antioxidants / Q000378:metabolism; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis; D009363:Neoplasm Proteins; D010544:Peroxidases / Q000235:genetics / Q000378:metabolism*; D061105:Peroxiredoxin III; D054464:Peroxiredoxins; D017382:Reactive Oxygen Species / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D015533:Transcriptional Activation / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000975:Antioxidants; D001798:Blood Proteins; D006360:Heat-Shock Proteins; D016209:Interleukin-8; D009363:Neoplasm Proteins; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha; D010544:Peroxidases; C086065:PRDX2 protein, human; C516115:PRDX3 protein, human; D061105:Peroxiredoxin III; D054464:Peroxiredoxins