Calcium imaging: a primer for mycologists.
This article aims to encourage more fungal biologists to consider the imaging of cytoplasmic Ca2+ fluxes. Compared to other organisms, for fungi there have been remarkably few attempts to characterize the role of Ca2+ fluxes in signal transduction and general cellular activities, even though other approaches indicate that fungal growth and development are highly dependent upon Ca2+. The methodologies for imaging Ca2+ fluxes continue to develop rapidly. These methodologies are explained here in a style that should be accessible to a newcomer to the field, hopefully forming a bridge to the more complex methodological literature.
Hyde
G
G
Faculty of Life Sciences, University of New South Wales, Sydney, 2052, Australia. G.Hyde@UNSW.edu.au
D002118:Calcium / Q000032:analysis*; D004396:Coloring Agents; D005658:Fungi / Q000254:growth & development / Q000502:physiology*; D007477:Ions; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D015398:Signal Transduction / Q000502:physiology
D004396:Coloring Agents; D007477:Ions; D002118:Calcium