Zonal induction of mixed lineage kinase ZPK/DLK/MUK gene expression in regenerating mouse liver.
ZPK/DLK/MUK is a serine/theronine kinase believed to be involved in the regulation of cell growth and differentiation. To further explore the suggested participation of ZPK/DLK/MUK in this process, we examined the expression and cellular localization of ZPK/DLK/MUK mRNA in regenerating mouse liver following partial hepatectomy by ribonuclease protection assay and in situ hybridization. The steady-state level of APK/DLKMUK mRNA was very low in normal and sham-operated mouse livers, whereas a marked and transient increase was observed in the regenerating liver. While ZPK/DLK/MUK mRNAs were rarely detected in hepatocytes from all zones of the normal liver, hepatocytes of regenerating liver exhibit a gradient of expression ranging from low in the periportal zone, to intermediate in the mid-zone, to high in the pericentral zone. These findings demonstrate a transient stimulation of ZPK/DLK/MUK gene expression that correlates with the growth response of hepatocyte subpopulations in regenerating liver.
Douziech
M
M
Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Québec, Canada.
Grondin
G
G
Loranger
A
A
Marceau
N
N
Blouin
R
R
D000818:Animals; D015870:Gene Expression; D006498:Hepatectomy; D017403:In Situ Hybridization; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology*; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D011494:Protein Kinases / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014018:Tissue Distribution
D012333:RNA, Messenger; D011494:Protein Kinases; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C090757:mitogen-activated protein kinase kinase kinase 12