FISH mapping and inter-Alu fingerprinting define the YAC contig map around the centromeric region of human chromosome 18.
Previous reports concerning the location of D18S44 with respect to the centromere have been ambiguous. Also, it has not been possible, based on formerly reported markers, to show that contigs WC18.0 and WC18.1 overlap. However, the data presented here definitively show, using FISH technology, that D18S44 (located on WC18.0) maps to proximal 18q. Furthermore, inter-Alu fingerprinting shows a clear overlap between WC18.0 and WC18.1, thereby establishing a complete contig between D18S44 and markers from WC18.1.
Greer
W L
WL
Department of Pathology, Queen Elizabeth II Health Sciences Centre, Halifax, NS, Canada. wgreer@is.dal.ca
Dobson
M J
MJ
Neumann
P E
PE
Girouard
G S
GS
Sparrow
S M
SM
Riddell
D C
DC
D002503:Centromere; D018244:Chromosomes, Artificial, Yeast; D002887:Chromosomes, Human, Pair 18; D016172:DNA Fingerprinting; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid