Enhancement of phagocytosis in mouse peritoneal macrophages by fragments of acidic fibroblast growth factor (aFGF).
To characterize the effects of acidic fibroblast growth factor (aFGF) in mouse peritoneal macrophages, the effects of aFGF fragments on phagocytosis were examined. Fragments that were tested included aFGF(1-15), aFGF(1-20), aFGF(1-29), Ala16-aFGF(1-29), aFGF(9-29) and aFGF(114-140). aFGF(1-29) induced an enhancement of phagocytosis in a dose-dependent manner and was more effective than any other fragments tested. Even in Ca2+-and Mg2+-free solutions, phagocytosis was enhanced by aFGF(1-29). However, the enhancement induced by aFGF(1-29) was completely inhibited in the presence of mannan (4 mg/ml). Furthermore, the enhancement of phagocytosis by aFGF(1-29) was suppressed by heparin (100 microg/ml). The results of the present study suggest that the active region of aFGF that is responsible for the enhancement of phagocytosis corresponds to residues 15-29 and that phagocytosis, which is modulated by aFGF, is independent of extracellular Ca2+ and is mediated by mannose receptors.
Ichinose
M
M
Department of Physiology, Shimane Medical University, Izumo, Japan. michinos@shimane-med.ac.jp
Sawada
M
M
Sasaki
K
K
Oomura
Y
Y
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology*; D005434:Flow Cytometry; D006493:Heparin / Q000494:pharmacology; D037181:Lectins, C-Type; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D051379:Mice; D008807:Mice, Inbred BALB C; D010446:Peptide Fragments / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000502:physiology
D000925:Anticoagulants; D037181:Lectins, C-Type; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D010446:Peptide Fragments; D011956:Receptors, Cell Surface; D016220:Fibroblast Growth Factor 1; D006493:Heparin; D002118:Calcium