title: A novel phosphorylated lipid counteracts activation of the renal plasma membrane (Ca2+ + Mg2+)ATPase by endogenous phosphatidylinositol-4-phosphate.
abstract: The plasma membrane (Ca2+ + Mg2+)ATPase is activated by acidic phospholipids in reconstituted systems. In this report it is shown that reversible phosphorylation of endogenous phosphatidylinositol regulates the renal plasma membrane (Ca2+ + Mg2+)ATPase, and that a novel phosphorylated lipid that can be isolated from the same membrane strongly counteracts the stimulatory effect of phosphatidylinositol-4-phosphate.
authors.affiliation: Departmento de Bioquímica Médica, Universidad Federal do Rio de Janeiro, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Guilherme
authors.forename: C
authors.initials: C
authors.lastname: Reis-Silva
authors.forename: J H
authors.initials: JH
authors.lastname: Moraes-Albuquerque
authors.forename: M M
authors.initials: MM
authors.lastname: Oliveira
authors.forename: A
authors.initials: A
authors.lastname: Vieyra
mesh_terms: D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004789:Enzyme Activation; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000201:enzymology*; D008563:Membrane Lipids / Q000302:isolation & purification / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D012969:Sodium Fluoride / Q000494:pharmacology
chemical_list: D008563:Membrane Lipids; D018129:Phosphatidylinositol Phosphates; C037178:phosphatidylinositol 4-phosphate; D012969:Sodium Fluoride; D017301:Ca(2+) Mg(2+)-ATPase