title: Fiber-type-dependent expression of adenovirus-mediated transgene in mouse skeletal muscle fibers.
abstract: We show the efficient transduction and expression of the lacZ gene in the skeletal muscle of adult C57BL/10ScSn mice after adenovirus-mediated gene transfer. Of the myofibers in the tibialis anterior muscle 62% were beta-galactosidase positive after injection of the lacZ gene under the control of the chicken beta-actin promoter and the cytomegalovirus enhancer. The transduced gene was preferentially expressed in type IIA and IIX fibers, which were richer in oxidative enzymes than type IIB fibers.
authors.affiliation: National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hagiwara
authors.forename: A
authors.initials: A
authors.lastname: Ishii
authors.forename: I
authors.initials: I
authors.lastname: Nonaka
authors.forename: T
authors.initials: T
authors.lastname: Kikuchi
authors.forename: S
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000199:Actins / Q000235:genetics; D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002645:Chickens; D004742:Enhancer Elements, Genetic / Q000235:genetics; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D007150:Immunohistochemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018485:Muscle Fibers, Skeletal / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism*; D009928:Organ Specificity; D011401:Promoter Regions, Genetic; D019076:Transgenes / Q000235:genetics*; D001616:beta-Galactosidase / Q000032:analysis / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000199:Actins; D001616:beta-Galactosidase