title: Stabilization of Tc-99m D,L-HMPAO preparations as a leucocyte labelling agent.
abstract: An attempt was made to use stabilized Tc-99m D,L-HMPAO (S-HMPAO) to label leucocytes. The radiochemical purity of Tc-99m D,L-HMPAO, labelling efficiency of leucocytes, cell viability of labelled leucocytes, and stability of S-HMPAO labelled leucocytes were calculated. In comparison with commercial Tc-99m D,L-HMPAO (C-HMPAO) without stabilization, immediately, at 0.5, 1, 2, 4, and 6 h after HMPAO preparation, the radiochemical purity of S-HMPAO and the labelling efficiencies of S-HMPAO labelled leucocytes were higher. S-HMPAO is more stable than C-HMPAO and can provide higher leucocyte labelling efficiency. S-HMPAO, therefore, has the potential to replace C-HMPAO as a leucocyte-labelling agent.
authors.affiliation: Department of Nuclear Medicine, Taichung Veterans General Hospital, Taiwan, ROC.
authors.forename: C H
authors.initials: CH
authors.lastname: Kao
authors.forename: W T
authors.initials: WT
authors.lastname: Huang
authors.forename: C S
authors.initials: CS
authors.lastname: Tsai
mesh_terms: D002470:Cell Survival; D004355:Drug Stability; D006801:Humans; D007700:Kinetics; D007962:Leukocytes / Q000166:cytology / Q000378:metabolism*; D019690:Technetium Tc 99m Exametazime / Q000097:blood* / Q000737:chemistry*
chemical_list: D019690:Technetium Tc 99m Exametazime