Expression of adhesion molecules in cultured human nasal mucosal microvascular endothelial cells activated by interleukin-1 beta or tumor necrosis factor-alpha: effects of dexamethasone.
Adhesion molecules of microvascular endothelial cells play a key role in the inflammatory processes involved in nonallergic sinusitis. We investigated the cytokine-regulated expression of intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the effect of dexamethasone on these expressions in cultured human nasal microvascular endothelial cells (HNMEC). ICAM-1 was enhanced, and E-selectin and VCAM-1 were induced in a dose-dependent fashion following stimulation with IL-1beta or TNF-alpha. HNMEC differed from human umbilical vein endothelila cells in that (1) maximal upregulation of ICAM-1 expression induced by IL-1beta or TNF-alpha required more time (2) TNF-alpha was more potent than IL-1beta in VCAM-1 expression, and (3) dexamethasone inhibited the upregulation of E-selectin expression alone. These findings contribute to a better understanding of the characteristic features of leukocyte infiltration into inflamed tissue and the effect of glucocorticoid in nonallergic chronic sinusitis.
Yamamoto
Y
Y
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Ikeda
K
K
Watanabe
M
M
Shimomura
A
A
Suzuki
H
H
Oshima
T
T
Imamura
Y
Y
Ohuchi
K
K
Takasaka
T
T
D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000494:pharmacology* / Q000502:physiology; D008833:Microcirculation / Q000187:drug effects; D009297:Nasal Mucosa / Q000098:blood supply* / Q000166:cytology / Q000187:drug effects*; D011446:Prospective Studies; D011897:Random Allocation; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000502:physiology; D014471:Umbilical Veins
D015815:Cell Adhesion Molecules; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D003907:Dexamethasone