Biological activities of C3 beta c, a novel neutrophil chemoattractant derived from the beta-chain of rat complement C3.
Biological activities of C3 beta c, which is a C-terminal fragment of the beta-chain of rat complement C3, have been studied by in vivo and in vitro experiments. C3 beta c was purified as a novel neutrophil chemoattractant from the exudate of the chronic phase of rat carrageenin-induced inflammation. The purified C3 beta c induced neutrophil chemotaxis in vivo when C3 beta c was injected into the preformed air-pouch on the back of rats. C3 beta c transiently increased the intracellular free Ca2+ concentration of neutrophils and enhanced the adhesion of neutrophils to fibrinogen in vitro, suggesting that C3 beta c has the ability to express an adhesion molecule of rat neutrophils. In addition, C3 beta c at low concentrations (10(-10)-10(-11) M) stimulated rat macrophages to produce cytokine-induced neutrophil chemoattractant-2, a member of the interleukin-8 family. Furthermore, C3 beta c enhanced vascular permeability in vivo, which is suppressed by cyproheptadine, suggesting that C3 beta c may have the characteristics of an anaphylatoxin. Our results suggest that C3 beta c contributes to oedema formation and neutrophil accumulation at inflammatory sites in rats.
Nakagawa
H
H
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Sunada
Y
Y
Ando
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002199:Capillary Permeability / Q000276:immunology; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000096:biosynthesis / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology*; D005340:Fibrinogen / Q000378:metabolism; D006133:Growth Substances / Q000096:biosynthesis; D036341:Intercellular Signaling Peptides and Proteins; D008262:Macrophage Activation / Q000276:immunology; D008264:Macrophages / Q000378:metabolism; D008297:Male; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology; D051381:Rats; D017208:Rats, Wistar
D019743:Chemokines, CXC; D002630:Chemotactic Factors; D003176:Complement C3; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010446:Peptide Fragments; D005340:Fibrinogen; D002118:Calcium