Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny.
Development-dependent mRNA expression of the chloride channels ClC-2 and ICln was studied by quantitative reverse transcriptase-polymerase chain reaction in rat ureteric bud and cortical collecting duct primary monolayer cultures. Abundance of ClC-2 mRNA increased in ureteric bud cells between embryonic day 15 (E15) and E17, peaked at postnatal day 3 (P3), and was down-regulated at P7 when morphogenesis is complete, suggesting a specific embryonic function. Expression of ICln mRNA, in contrast, up-regulated continuously with development.
Huber
S
S
Physiologisches Institut, Ludwig-Maximilians-Universität, Germany.
Braun
G
G
Schröppel
B
B
Horster
M
M
D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels / Q000235:genetics*; D004847:Epithelial Cells / Q000737:chemistry / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D007473:Ion Channels; D007685:Kidney Tubules, Collecting / Q000737:chemistry / Q000166:cytology / Q000196:embryology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014513:Ureter / Q000737:chemistry / Q000166:cytology / Q000196:embryology*
D018118:Chloride Channels; C498729:Clns1a protein, rat; D007473:Ion Channels; D012333:RNA, Messenger