[Cerebrospinal fluid proteins in the diagnosis of disorders of the blood-cerebrospinal fluid barrier in central nervous system diseases].
BACKGROUND
Many neurological diseases are connected with the dysfunction of blood-CSF barrier. The quantitative determination of CSF proteins has already been used in the diagnosis of barrier impairments and inflammatory diseases of the central nervous system.


METHODS
Serum and CSF, totaling 264 samples, were obtained from 15 controls and 117 patients with various diseases of the nervous system. Laurell's electroimmunoassay was used for estimation of albumin and IgG levels in serum and CSF. CSF-protein profile was evaluated according to Reiber's graph for the evaluation of the CSF-protein profile.


RESULTS
The graph for the protein profile can be divided into 5 functionally different parts (1--normal range, 2, 3, 4--different types of barrier dysfunctions and 5--local humoral response in CNS without any barrier impairment). There was a good correlation of CSF-protein profiles and neurological diseases in our group of patients.


CONCLUSIONS
According to our results, Reiber's graph was helpful for the diagnosis of blood-CSF-barrier dysfunctions. The graph has the following advantages: a) possibility of simultaneous assessment of the functional state of blood-CSF-barrier and the inflammatory response of the CNS, b)sensitivity for the determination of pathological local IgG-production in CNS and c) minimal number of protein assays necessary.
Turecký
L
L
Ustav lekárskej chémie, biochémie a klinickej biochémie Lekárskej fakulty Univerzity Komenského v Bratislave.
Kalina
P
P
Uhlíková
E
E
Procházková
L
L
D001812:Blood-Brain Barrier; D001927:Brain Diseases / Q000134:cerebrospinal fluid / Q000503:physiopathology*; D002555:Cerebrospinal Fluid / Q000502:physiology; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans
D002556:Cerebrospinal Fluid Proteins