Quantitation of fumonisins in corn by HPLC with o-phthalaldehyde postcolumn derivatization and their identification by LC/MS.
Fumonisins B1 (FB1) and B2 (FB2) were isocratically separated on a fluorocarbon column without using an ion pair reagent and nonvolatile buffer during the HPLC and were detected by an o-phthalaldehyde postcolumn derivatization system using a fluorescence detector. The minimum detectable concentrations of FB1 and FB2 in corn by this system were 0.01 micrograms/g and 0.01 micrograms/g, respectively. The separated fumonisins were further identified by a directly interfaced ion trap MS using electrospray ionization. FB1 and FB2 in naturally contaminated corn were identified in the selective ion monitoring mode at concentrations of 3.75 micrograms/g and 1.44 micrograms/g, respectively.
Akiyama
H
H
National Institute of Health Sciences, Tokyo, Japan.
Uraroongroj
M
M
Miyahara
M
M
Goda
Y
Y
Toyoda
M
M
D002264:Carboxylic Acids / Q000032:analysis* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D037341:Fumonisins; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification; D003313:Zea mays / Q000737:chemistry* / Q000382:microbiology; D009764:o-Phthalaldehyde
D002264:Carboxylic Acids; D037341:Fumonisins; D009183:Mycotoxins; C056934:fumonisin B2; C073063:fumonisin B3; C056933:fumonisin B1; D009764:o-Phthalaldehyde