Alternations of diacylglycerol kinase in streptozotocin-induced diabetic rats.
Dysfunction of organs has been reported in diabetic rats, suggesting an association with changes in intracellular signal transduction pathways including phosphatidylinositol (PI) turnover. Diacylglycerol (DG) kinase catalyses the phosphorylation of DG, which is considered to play a major physiological role in the metabolism of the intracellular messenger DG. However, no relation between DG kinase activity and any disease in mammalian tissue has been reported to date. In the present study, we investigated whether the changes in DG kinase activity are related to diabetes. Basal resting level of DG kinase activity changed in tissue isolated from diabetic rats. Decreases in resting activity detected in aorta and kidney and agonist-induced responses differed between these tissues. Submaximal increases in basal activity also were detected in vas deferens and hepatocytes. These changes in DG kinase activity resemble the functional changes associated with complications of diabetes, suggesting that changes in PI turnover followed by DG kinase activity are a key element in the complications. It is the first study about the changes in DG kinase activity in mammalian disease.
Nobe
K
K
Department of Pharmacology, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan. kojinobe@pharm.showa-u.ac.jp
Sakai
Y
Y
Momose
K
K
D000818:Animals; D001011:Aorta / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000201:enzymology*; D019852:Diacylglycerol Kinase / Q000378:metabolism*; D004075:Diglycerides / Q000378:metabolism; D007553:Isotope Labeling; D007672:Kidney Cortex / Q000378:metabolism; D008099:Liver / Q000166:cytology; D008297:Male; D010710:Phosphates / Q000378:metabolism; D010712:Phosphatidic Acids / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D051381:Rats; D013311:Streptozocin; D014649:Vas Deferens / Q000378:metabolism
D004075:Diglycerides; D010710:Phosphates; D010712:Phosphatidic Acids; D010761:Phosphorus Radioisotopes; C084527:dioctanoylphosphatidic acid; C044295:1,2-dioctanoylglycerol; D013311:Streptozocin; D019852:Diacylglycerol Kinase