PCR-based rapid detection of Mycobacterium tuberculosis in blood from immunocompetent patients with pulmonary tuberculosis.
A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for the circulating DNA copies of M. tuberculosis complex.
Ahmed
N
N
Molecular Biology Unit, National Dairy Research Institute, Karnal 132001, India. niyaz@mailcity.com
Mohanty
A K
AK
Mukhopadhyay
U
U
Batish
V K
VK
Grover
S
S
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000097:blood*; D018023:HIV Seronegativity; D006801:Humans; D007121:Immunocompetence; D007194:India; D007962:Leukocytes / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D014397:Tuberculosis, Pulmonary / Q000097:blood / Q000175:diagnosis*
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial