Calprotectin and lactoferrin levels in the gingival crevicular fluid of children.
The purpose of this study was to determine the levels of calprotectin and lactoferrin, 2 microbiostatic proteins, in the gingival crevicular fluid (GCF) of normal children. The children represented a population, primarily underprivileged, seeking care at a regional dental health care center. GCF was collected from Ramfjord teeth (or their deciduous equivalent). GCF volume was quantified by conductance. Calprotectin and lactoferrin levels were quantified by sandwich ELISA, and found to have a mean value of 70.8+/-94.2 microg/mL and 68.2+/-108.7 microg/mL, respectively. The levels of calprotectin and lactoferrin varied directly with one another and inversely with the amount of fluid obtained in a 20-second sampling period. The mean levels were at or above the minimum inhibitory concentration determined in vitro.
Miyasaki
K T
KT
Section of Oral Biology and the Dental Research Institute, UCLA School of Dentistry, Los Angeles, CA 90095-1668, USA. kmiyasak@ucla.edu
Voganatsi
A
A
Huynh
T
T
Marcus
M
M
Underwood
S
S
D000293:Adolescent; D000935:Antifungal Agents / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D002135:Calcium-Binding Proteins / Q000032:analysis*; D002648:Child; D019140:Dental Care for Children; D004553:Electric Conductivity; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007781:Lactoferrin / Q000032:analysis*; D039841:Leukocyte L1 Antigen Complex; D008297:Male; D008489:Medical Indigency; D008562:Membrane Glycoproteins / Q000032:analysis*; D019006:Neural Cell Adhesion Molecules / Q000032:analysis*; D012636:Secretory Rate
D000935:Antifungal Agents; D000954:Antigens, Surface; D002135:Calcium-Binding Proteins; D039841:Leukocyte L1 Antigen Complex; D008562:Membrane Glycoproteins; D019006:Neural Cell Adhesion Molecules; D007781:Lactoferrin