[Relationship between human papillomavirus E7 protein and Rb gene product in fresh tissues of squamous cervical carcinoma].
OBJECTIVE
To study the possibility of complex formation of the E7 protein encoded by human papillomavirus (HPV) binding to retinoblastoma gene product (pRb) in fresh samples of squamous cervical carcinoma (SCC).


METHODS
HPV 6/11, 16, 18, 33 DNA were detected in 40 samples of by polymerase chain reaction technique (PCR). The complexes of HPV E7-pRb were examined in fresh tissues of HPV contained SCC through capture enzyme linked immunosorbent assay (capture-ELISA).


RESULTS
45.0% (18/40) of the samples were proved to be HPV positive by PCR. Out of 18 samples with HPV positive samples, the complexes of HPV E7-pRb were detected in 9 cses, including 1 HPV18 positive and 8 HPV16 positives. The complexes of HPV E7-pRb were not found in 2 cases of positive HPV 6/11. No correlation was observed between the E7 protein binding to pRb and the histological grade of cervical carcinoma (P < 0.05). There was correlation with clinical staging, the number of cases showing that the E7 protein pRb complex in stage I was significantly higher than that in stages II-IV (P < 0.05).


CONCLUSIONS
The complex of "high risk" HPV E7-pRb was demonstrated in fresh tissues of SCC. There is no correlation between the complex and histological grade of SCC. The complex formation may occur in the early developmental stage of cervical cancer.
Second Affiliated Hospital, West University of Medical Sciences, Chengdu.
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P
Wang
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Peng
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Wang
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins; D005260:Female; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D027383:Papillomaviridae / Q000302:isolation & purification*; D050725:Papillomavirus E7 Proteins; D016160:Retinoblastoma Protein / Q000032:analysis*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000821:virology
D004279:DNA, Viral; D004268:DNA-Binding Proteins; C052633:E7 protein, Human papillomavirus type 18; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D016160:Retinoblastoma Protein; C059731:oncogene protein E7, Human papillomavirus type 16