Scanning near-field optical microscopy in cell biology and microbiology.
Scanning a point light source in close proximity over a sample and recording the scattered or transmitted light intensity point by point allows one to record optical images with a resolution not limited by diffraction. An overview of this technique called scanning near-field optical microscopy (SNOM or NSOM) is given with emphasis on cell- and microbiology. After an introduction, where the basic features of the technique are explained, illustrative examples are presented, such as a HeLa cell, fluorescence labelled human chromosomes, super resolution fluorescence imaging, single molecule imaging and fluorescence resonance energy transfer between a single pair of dye molecules.
Universität-Gesamthochschule Siegen, Germany.
A J
AJ
Meixner
H
H
Kneppe
D002877:Chromosomes, Human; D005456:Fluorescent Dyes; D006367:HeLa Cells / Q000648:ultrastructure; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008828:Microbiological Techniques / Q000295:instrumentation; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000295:instrumentation / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation / Q000379:methods; D008967:Molecular Biology / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
D005456:Fluorescent Dyes