A subtractive cDNA library from an identified regenerating neuron is enriched in sequences up-regulated during nerve regeneration.
We have constructed a subtractive cDNA library from regenerating Retzius cells of the leech, Hirudo medicinalis. It is highly enriched in sequences up-regulated during nerve regeneration. Sequence analysis of selected recombinants has identified both novel sequences and sequences homologous to molecules characterised in other species. Homologies include alpha-tubulin, a calmodulin-like protein, CAAT/enhancer-binding protein (C/EBP), protein 4.1 and synapsin. These types of proteins are exactly those predicted to be associated with axonal growth and their identification confirms the quality of the library. Most interesting, however, is the isolation of 5 previously uncharacterised cDNAs which appear to be up-regulated during regeneration. Their analysis is likely to provide new information on the molecular mechanisms of neuronal regeneration.
Sussex Centre for Neuroscience, Sussex University, Falmer, UK.
S
S
Korneev
A
A
Fedorov
R
R
Collins
S E
SE
Blackshaw
J A
JA
Davies
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D022762:CCAAT-Enhancer-Binding Proteins; D002147:Calmodulin / Q000737:chemistry; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000737:chemistry; D015723:Gene Library; D007865:Leeches / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009416:Nerve Regeneration / Q000235:genetics / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D009474:Neurons / Q000502:physiology*; D009479:Neuropeptides; D009687:Nuclear Proteins / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016704:Synapsins / Q000737:chemistry; D014404:Tubulin / Q000737:chemistry
D022762:CCAAT-Enhancer-Binding Proteins; D002147:Calmodulin; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D016704:Synapsins; D014404:Tubulin; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1