[Comparison of the results of the Etest and the method for determining minimum inhibitory concentrations in solid media for penicillin G, amoxicillin, and cefotaxime for S. pneumoniae. A multicenter study].
In 1996-1997 a multicentre study was carried out on 450 Streptococcus pneumoniae strains to compare the MICs and susceptibility categories obtained with the Etest (AB Biodisk) used under routine conditions in 22 hospital laboratories in the Rhône-Alpes region, France, with those obtained by the reference technique of agar dilution performed in a single coordinating centre. Each laboratory detected penicillin resistant pneumococci (PRP) by the oxacillin disk method (1 microgram and 5 micrograms) and determined the MICs of penicillin G (PG), amoxycillin (AMX) and cefotaxime (CTX) by the Etest. All the PRP strains were collected in the coordinating centre where MICs were carried out. The strains were classified as susceptible (S), intermediate (I) and resistant (R) according to the CASFM criteria (Comité de l'Antibiogramme de la Société Française de Microbiologie). The concordance results based on susceptibility categories are as follows: PG = 67.6%, AMX = 63.6%, CTX = 71.5%. Minor errors are as follows: PG = 31.2%, AMX = 36%, CTX = 28.5%. Major and very major errors are rare (0% to 0.6%). Agreement within 1 log2 dilution was obtained for about 80% of the strains. The minor errors results from strains clustering near the breakpoints 1 mg/l (PG) and 0.5 mg/l (AMX, CTX), and from practical difficulties in routine use of the Etest. These discrepancies may result in severe therapeutic problems. This study confirms the limits of the Etest. The authors insist on standardization and rigorous use of the Etest under routine conditions.
Clavier
B
B
Laboratorie de Biologie Médicale, Hôpital d'Instruction des Armées Desgenettes, Lyon, France.
Perrier-Gros-Claude
J D
JD
Foissaud
V
V
Dumas
M
M
Aubert
G
G
Barbé
G
G
Bland
S
S
Boucaud Maitre
Y
Y
Boyer
M
M
Chomarat
M
M
Clergeau
P
P
Delubac
F
F
Févre
D
D
Fuhrmann
C
C
Gravagnat
B
B
Letouzey
M N
MN
Mandjee
A
A
Martelet
P
P
Meley
R
R
Reverdy
M E
ME
Ros
A
A
Roure
C
C
Sabot
O
O
Tixier
A
A
Thierry
J
J
D000658:Amoxicillin / Q000494:pharmacology*; D002439:Cefotaxime / Q000494:pharmacology*; D018550:Cephalosporin Resistance; D003470:Culture Media; D004058:Diffusion; D005069:Evaluation Studies as Topic; D005188:False Negative Reactions; D005189:False Positive Reactions; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D010400:Penicillin G / Q000494:pharmacology*; D010403:Penicillin Resistance; D011786:Quality Control; D015203:Reproducibility of Results; D013296:Streptococcus pneumoniae / Q000187:drug effects*
D003470:Culture Media; D000658:Amoxicillin; D002439:Cefotaxime; D010400:Penicillin G
Comparaison des résultats du Etest et de la méthode de détermination des concentrations minimales inhibitrices en milieu solide pour la pénicilline G, l'amoxicilline et le céfotaxime chez S. pneumoniae. Une étude multicentrique.