The cytoplasmic zinc finger protein ZPR1 accumulates in the nucleolus of proliferating cells.
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Z
Z
Galcheva-Gargova
L
L
Gangwani
K N
KN
Konstantinov
M
M
Mikrut
S J
SJ
Theroux
T
T
Enoch
R J
RJ
Davis
D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000378:metabolism / Q000648:ultrastructure; D003001:Cloning, Molecular; D016384:Consensus Sequence; D017124:Conserved Sequence; D004815:Epidermal Growth Factor / Q000494:pharmacology; D015723:Gene Library; D005816:Genetic Complementation Test; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D026901:Membrane Transport Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
D002352:Carrier Proteins; D047908:Intracellular Signaling Peptides and Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C490986:ZPR1 protein, S cerevisiae; C099746:ZPR1 protein, human; C490987:Zfp259 protein, mouse; D004815:Epidermal Growth Factor