New quantitative methods for detection of feline parvovirus (FPV) and virus neutralizing antibody against FPV using a feline T lymphoid cell line.
Previously, we reported that a feline T lymphoid cell line, FL74 cells, was very sensitive to feline parvovirus (FPV) infection. In the present study, we developed new quantitative methods for detection of FPV and virus neutralizing antibody against FPV using FL74 cells. The methods presented here were very simple and applicable to both canine parvovirus and feline panleukopenia virus.
Ikeda
Y
Y
Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Miyazawa
T
T
Kurosawa
K
K
Naito
R
R
Hatama
S
S
Kai
C
C
Mikami
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002415:Cats; D002460:Cell Line; D004285:Dogs; D028301:Feline Panleukopenia Virus / Q000276:immunology* / Q000302:isolation & purification / Q000502:physiology*; D009500:Neutralization Tests; D017993:Parvovirus, Canine / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology*
D000914:Antibodies, Viral