Photolysis and membrane lipid peroxidation of human erythrocytes by m-chloroperbenzoic acid.
The aim of the present work was to investigate the biological hazard of photooxidation products of m-chloroperbenzoic acid (mCPBA), as a novel photo-sensitizer, on lysis and membrane lipid peroxidation of human red blood cells (RBC). The photohemolysis activity of mCPBA oxidative products was concentration- and exposure time-dependent. Ten minutes photoexposure time and 100 micromol/L of mCPBA concentration were optimum to study the effect of generated superoxide (O2.-) and hydroxyl (.OH) radicals on RBC. The hemolytic effect of mCPBA was highly significantly inhibited by formate (as an .OH radical scavenger) compared with the partial inhibition effect of SOD-like Cu(II) complex (as O2.- radical Scavenger). The MDA value (an end product of membrane lipid peroxidation of RBC) induced by mCPBA was highly significantly decreased by formate. The generation of O2.- radicals by mCPBA was also confirmed by the partial hemolytic effect of phenazine methosulfate (PMS., O2.- radical generation). The data suggest the molecular mechanism of the oxygen radical species (O2.- and .OH through the photosensitization reaction of mCPBA and explain that hydroxyl radicals (.OH) play an active role in the photohemolysis process and peroxidation of membrane lipids of human erythrocytes.
Abou-Seif
M A
MA
Chemistry Department, Faculty of Science, Mansoura University, Egypt.
Elgendy
E M
EM
D002723:Chlorobenzoates / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism*; D005561:Formates / Q000494:pharmacology; D006461:Hemolysis; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism / Q000494:pharmacology; D008027:Light; D015227:Lipid Peroxidation; D008315:Malondialdehyde / Q000097:blood; D008563:Membrane Lipids / Q000378:metabolism*; D010084:Oxidation-Reduction; D010782:Photolysis; D017319:Photosensitizing Agents; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D002723:Chlorobenzoates; D005561:Formates; D008563:Membrane Lipids; D017319:Photosensitizing Agents; D017392:Thiobarbituric Acid Reactive Substances; C030544:formic acid; D013481:Superoxides; D017665:Hydroxyl Radical; D008315:Malondialdehyde; C000433:3-chloroperbenzoic acid