K-ras mutations in stools and tissue samples from patients with malignant and nonmalignant pancreatic diseases.
Mutant-enriched PCR and reverse dot blot hybridization in microplates were applied for examining K-ras status in stools and tissue samples from patients with pancreatic tumors and chronic pancreatitis. In tissue samples, K-ras mutations were found in 32 of 35 cases of ductal adenocarcinoma, in 5 of 7 periampullary cancers, in 1 cystadenocarcinoma, and in 3 of 5 patients with chronic pancreatitis. In stools, mutated K-ras was seen in 10 of 25 cases of ductal adenocarcinoma, in 1 case of cystadenocarcinoma, and in 2 of 6 cases of chronic pancreatitis. These data indicate that the K-ras status of stool samples may help identify pancreatic carcinoma and persons at risk for cancer development; however, it does not allow discrimination of malignant from nonmalignant diseases.
Institute of Laboratory Medicine and Pathobiochemistry, Medical Faculty Charité, Humboldt-University, Berlin, Germany. christoph.berndt@charite.de
C
C
Berndt
K
K
Haubold
F
F
Wenger
B
B
Brux
J
J
Müller
P
P
Bendzko
T
T
Hillebrand
E
E
Köttgen
J
J
Zanow
D000230:Adenocarcinoma / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D018395:CA-19-9 Antigen / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000032:analysis; D002908:Chronic Disease; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004273:DNA, Neoplasm / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000737:chemistry*; D006801:Humans; D007124:Immunoenzyme Techniques; D015689:Oncogene Protein p21(ras) / Q000032:analysis / Q000235:genetics*; D010179:Pancreas / Q000737:chemistry*; D010183:Pancreatic Ducts; D010190:Pancreatic Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology; D010195:Pancreatitis / Q000235:genetics*; D017354:Point Mutation; D016133:Polymerase Chain Reaction
D014408:Biomarkers, Tumor; D018395:CA-19-9 Antigen; D002272:Carcinoembryonic Antigen; D004273:DNA, Neoplasm; D004247:DNA; D015689:Oncogene Protein p21(ras)