Development of in vitro screening system for assessment of antifilarial activity of compounds.
Evaluation of antifilarial activity of new potential agents in vivo is extremely time consuming and uneconomic. In the present study effort has been made to develop an in vitro screening method using Acanthocheilonema viteae, a subcutaneously dwelling rodent filariid with anaerobic metabolic characteristics like human filariids, W. Bancrofti/Brugia malayi as test parasite. Motility test and tetrazolium (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) based colorimetric assay were used as parameters in in vitro assay. Results showed that 92.3% of compounds (in vivo active) could be picked up in the in vitro assay when both adults and microfilarae (mf) were used simultaneously. Mf and adult stages separately detected, respectively, 84.6 and 69.2% of in vivo active compounds. The adults and mf separately and both the life stages together exhibited, respectively, 80.0, 50.0 and 80.0% false positive results in the in vitro test with in vivo inactive compounds. It is felt that mf stage when used in in vitro test using motility and MTT assays as parameters would be useful in primary screening of new potential filaricides.
Mukherjee
M
M
Division of Parasitology, Central Drug Research Institute, Lucknow, India.
Misra
S
S
Chatterjee
R K
RK
D000818:Animals; D003124:Colorimetry; D004396:Coloring Agents / Q000737:chemistry; D004153:Dipetalonema / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D004154:Dipetalonema Infections / Q000188:drug therapy / Q000469:parasitology; D005188:False Negative Reactions; D005189:False Positive Reactions; D005260:Female; D005369:Filaricides / Q000494:pharmacology*; D008297:Male; D008842:Microfilariae / Q000187:drug effects / Q000502:physiology; D009068:Movement / Q000187:drug effects; D009115:Muridae; D010084:Oxidation-Reduction; D011237:Predictive Value of Tests; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
D004396:Coloring Agents; D005369:Filaricides; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue