[A recombinant autoantigen U 1 RNP 70,000: expression in E. coli. and its serodiagnostic application].
OBJECTIVE
To express the recombinant autoantigen U 1 RNP 70,000 and to evaluate its serodiagnostic value.


METHODS
We used molecular cloning techniques and immunoblotting technique.


RESULTS
The recombinant proteins had authentic U 1 RNP 70,000 antigenicity, with the molecular weight corresponding to their theoretical value. 112 of 115(97.4%) anti-70,000 positive sera(IBT) could be detected by r 70,000.


CONCLUSIONS
r 70,000 has good specificity and can be used in further detailed epitopes mapping. r 70,000 can be put into use for serodiagnostic detection.
Department of Rheumatology, Renji Hospital, Shanghai Second Medical University, Shanghai Immunology Institute.
F
F
Xue
N
N
Shen
Y
Y
Lu
D001324:Autoantigens / Q000096:biosynthesis*; D003001:Cloning, Molecular; D018604:Epitope Mapping; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000175:diagnosis*; D008947:Mixed Connective Tissue Disease / Q000175:diagnosis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D012595:Scleroderma, Systemic / Q000175:diagnosis; D055517:snRNP Core Proteins
D001324:Autoantigens; D011993:Recombinant Fusion Proteins; D055517:snRNP Core Proteins