In vivo mutational analysis of bacteriophage Mu operators.
In bacteria lysogenic for bacteriophage Mu, the phage repressor binds to a tripartite operator region, O1,O2,O3, to repress the lytic promoter pE, located in O2, and negatively autoregulate its own synthesis at the pCM promoter located in O3. We isolated and characterized operator mutations which lead to derepression of pE. Their location in the first and third repressor-consensus-binding sequences in O2 confirms the importance of these sites for repressor/operator interactions.
Laboratoire de Génétique des Procaryotes, Université Libre de Bruxelles, Rhode St Genèse Belgium.
L
L
Desmet
M J
MJ
Gama
J E
JE
Laachouch
I
I
Petrescu
P
P
Rousseau
A
A
Toussaint
D010583:Bacteriophage mu / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D004279:DNA, Viral / Q000378:metabolism; D015967:Gene Expression Regulation, Viral; D008969:Molecular Sequence Data; D009875:Operator Regions, Genetic / Q000235:genetics*; D017354:Point Mutation / Q000235:genetics*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D012097:Repressor Proteins / Q000378:metabolism; D017384:Sequence Deletion
D004279:DNA, Viral; D012097:Repressor Proteins