A novel photointermediate of octopus rhodopsin activates its G-protein.
The photointermediate of octopus rhodopsin responsible for G-protein activation was examined by a GTPgammaS-binding assay in a reconstituted system with purified rhodopsin and photoreceptor G-protein. When octopus rhodopsin alone was incubated in the dark after illumination, its ability to stimulate GTPgammaS-binding by the G-protein decreased in a time-dependent manner. We associate this decay with the decay of a novel photointermediate, transient acid metarhodopsin, which lies between mesorhodopsin and acid metarhodopsin. Spectroscopic evidence for its existence was suggested by its effects on the turbidity of the vesicles. These results suggest that the transient acid metarhodopsin, not the stable final photoproduct, acid metarhodopsin, activates a G-protein in octopus photoreceptors.
Department of Life Science, Himeji Institute of Technology, Harima Science Garden City, Hyogo, Japan.
M
M
Nakagawa
S
S
Kikkawa
K
K
Tominaga
N
N
Tsugi
M
M
Tsuda
D000818:Animals; D003624:Darkness; D019204:GTP-Binding Proteins / Q000378:metabolism*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D007700:Kinetics; D008027:Light; D008871:Microvilli / Q000378:metabolism*; D049831:Octopodiformes; D017956:Photoreceptor Cells, Invertebrate / Q000378:metabolism*; D012243:Rhodopsin / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D013997:Time Factors
D016244:Guanosine 5'-O-(3-Thiotriphosphate); D012243:Rhodopsin; D019204:GTP-Binding Proteins