Liquid chromatographic determination of myocardial interstitial epinephrine.
This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring of epinephrine (Epi) in the myocardial interstitial space. The in vitro detection limit for Epi was 200 fg in a 50-microl injection. Using a cardiac dialysis technique, 60-microl dialysates were sampled from the myocardial interstitial space (6-min fractions). After an alumina procedure, the dialysate Epi concentration was measured using the HPLC-ED system. Although the basal Epi concentration was undetectable, local administration of desipramine increased Epi concentration of the dialysate to 38.1+/-18.5 pg/ml. This system affords a new possibility for estimating myocardial interstitial Epi level.
Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
T
T
Kawada
T
T
Yamazaki
T
T
Akiyama
T
T
Sato
T
T
Shishido
M
M
Sugimachi
M
M
Inagaki
J
J
Alexander
K
K
Sunagawa
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004837:Epinephrine / Q000032:analysis*; D005110:Extracellular Space / Q000737:chemistry*; D009206:Myocardium / Q000737:chemistry*; D011817:Rabbits; D012680:Sensitivity and Specificity
D004837:Epinephrine