Sequence elements involved in the rescue of IBV defective RNA CD-91.
Deletion mutagenesis has been used to identify essential regions for rescue of coronavirus defective RNAs (D-RNAs). Using this technique on a cloned IBV D-RNA CD-91, we have identified a region potentially important in its rescue. Comparing the sequence of D-RNAs rescued with those not rescued we have deduced that a 72 base region corresponding to base number 13,824 to 13,896 in the viral genome is required for rescue. This may be an IBV D-RNA packaging signal or a cis-acting element involved in replication. Further experiments and modification of our techniques will be required to differentiate between the two processes.
IAH, Compton, England.
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Dalton
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Penzes
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Wroe
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Stirrups
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Evans
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Shaw
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Brown
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Britton
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Cavanagh
D000818:Animals; D001483:Base Sequence; D003673:Defective Viruses / Q000235:genetics*; D001351:Infectious bronchitis virus / Q000235:genetics*; D012367:RNA, Viral
D012367:RNA, Viral