A SWI/SNF-related chromatin remodeling complex, E-RC1, is required for tissue-specific transcriptional regulation by EKLF in vitro.
Erythroid Krüppel-like factor (EKLF) is necessary for stage-specific expression of the human beta-globin gene. We show that EKLF requires a SWI/SNF-related chromatin remodeling complex, EKLF coactivator-remodeling complex 1 (E-RC1), to generate a DNase I hypersensitive, transcriptionally active beta-globin promoter on chromatin templates in vitro. E-RC1 contains BRG1, BAF170, BAF155, and INI1 (BAF47) homologs of yeast SWI/SNF subunits, as well as a subunit unique to higher eukaryotes, BAF57, which is critical for chromatin remodeling and transcription with EKLF. E-RC1 displays functional selectivity toward transcription factors, since it cannot activate expression of chromatin-assembled HIV-1 templates with the E box-binding protein TFE-3. Thus, a member of the SWI/SNF family acts directly in transcriptional activation and may regulate subsets of genes by selectively interacting with specific DNA-binding proteins.
Armstrong
J A
JA
Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Bieker
J J
JJ
Emerson
B M
BM
D001665:Binding Sites; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000378:metabolism*; D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D005914:Globins / Q000235:genetics; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D046911:Macromolecular Substances; D009687:Nuclear Proteins / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D016601:RNA-Binding Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear / Q000737:chemistry; D000071796:SMARCB1 Protein; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051741:Kruppel-Like Transcription Factors; D046911:Macromolecular Substances; D009687:Nuclear Proteins; D016601:RNA-Binding Proteins; D017412:Ribonucleoprotein, U1 Small Nuclear; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; C101681:SMARCC1 protein, human; C101682:SMARCC2 protein, human; D014157:Transcription Factors; C080774:erythroid Kruppel-like factor; C089969:snf protein, Drosophila; D005914:Globins; C084108:SMARCA4 protein, human; D004265:DNA Helicases