Fluconazole disk diffusion susceptibility testing of Candida species.
We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-microgram fluconazole disks. With 25-microgram disks, zones of inhibition of >/=20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were </=8 microgram/ml, and with 50-microgram disks, zones of >/=27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 microgram/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.
Departments of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284, USA.
W R
WR
Kirkpatrick
T M
TM
Turner
A W
AW
Fothergill
D I
DI
McCarthy
S W
SW
Redding
M G
MG
Rinaldi
T F
TF
Patterson
D017088:AIDS-Related Opportunistic Infections / Q000188:drug therapy / Q000382:microbiology; D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects* / Q000302:isolation & purification; D002176:Candida albicans / Q000187:drug effects / Q000302:isolation & purification; D002177:Candidiasis / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D004352:Drug Resistance, Microbial; D005069:Evaluation Studies as Topic; D015725:Fluconazole / Q000494:pharmacology*; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000706:statistics & numerical data; D009172:Mycology / Q000379:methods / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity
D000935:Antifungal Agents; D015725:Fluconazole