Overexpression of glucose transporter protein 5 in sciatic nerve of streptozotocin-induced diabetic rats.
The localization and the expression level of glucose transporter 5 (GLUT 5), detected by immunohistochemical and Western blot analyses, and motor nerve conduction velocity (MNCV) in normal and streptozotocin (STZ)-induced diabetic rats were compared. The effects of insulin and recombinant human insulin-like growth factor-I (rhIGF-I) were also investigated. GLUT 5 was localized in Schwann cells and axons. GLUT 5 was overexpressed in both sites 5 weeks after STZ injection and MNCV was decreased significantly in STZ-induced diabetic rats as compared with normal rats. These deviations returned to normal rat level after 2 weeks medication with insulin or rhIGF-I, started 3 weeks after STZ injection. These results suggest that overexpression of GLUT 5 may be a trigger for diabetic neuropathy.
Molecular Biological Research Laboratories, Fujisawa Pharmaceutical, Osaka, Japan. takashiasada@po.fujisawa.co.jp
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T
Asada
S
S
Takakura
T
T
Ogawa
M
M
Iwai
M
M
Kobayashi
D000818:Animals; D015153:Blotting, Western; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology*; D003929:Diabetic Neuropathies / Q000503:physiopathology*; D015870:Gene Expression / Q000502:physiology; D051296:Glucose Transporter Type 5; D007004:Hypoglycemic Agents / Q000378:metabolism; D007328:Insulin / Q000502:physiology; D007334:Insulin-Like Growth Factor I / Q000502:physiology; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000032:analysis / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000737:chemistry / Q000502:physiology*; D015854:Up-Regulation / Q000502:physiology
D051296:Glucose Transporter Type 5; D007004:Hypoglycemic Agents; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D007334:Insulin-Like Growth Factor I