Seroconversion of type B to O erythrocytes using recombinant Glycine max alpha-D-galactosidase.
Recombinant alpha-D-galactosidase (rGal) from soybean (Glycine max) hydrolyzed the immunodominant alpha-D-galactose residue from the B epitope of red blood cells. This converted type B erythrocytes to type O which are "universally" transfusable. Type B red blood cells were obtained from four different donors and enzymatically converted. Cell function parameters, including red cell indices, pH, methemoglobin, carboxyhemoglobin, osmotic fragility, hemolysis, 2,3-diphosphoglycerate, cholinesterase, ATP, and antigen typing of treated cells were compared to controls. These pilot studies indicate that rGal could have potential biotechnical application in the production of universally transfusable red blood cells.
Vosnidou
N C
NC
Department of Biology, University of Missouri, Columbia 65212, USA.
Johnson
S A
SA
Mitra
M M
MM
Wells
D C
DC
Li
C Q
CQ
Evans
M L
ML
Harmata
M A
MA
Walker
J C
JC
Smith
D S
DS
D019794:2,3-Diphosphoglycerate / Q000097:blood; D000017:ABO Blood-Group System / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000097:blood; D002802:Cholinesterases / Q000097:blood; D004909:Erythrocyte Indices; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D005690:Galactose / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009996:Osmotic Fragility; D011994:Recombinant Proteins / Q000378:metabolism; D013025:Glycine max; D000519:alpha-Galactosidase / Q000378:metabolism*
D000017:ABO Blood-Group System; D011994:Recombinant Proteins; D019794:2,3-Diphosphoglycerate; D000255:Adenosine Triphosphate; D002802:Cholinesterases; D000519:alpha-Galactosidase; D005690:Galactose