Glucose transport in a methylotrophic yeast Hansenula polymorpha.
Glucose transport was studied in a methylotrophic yeast Hansenula polymorpha. Two kinetically different glucose transport systems were revealed in cells grown under different growth conditions. Glucose-repressed cells exhibited a low-affinity transport system (Km for glucose 1.75 mM) while glucose-derepressed and ethanol-grown cells had a high-affinity transport system (Km for glucose 0.05-0.06 mM). The high- and low-affinity transport systems differed in substrate specificity, sensitivity to pH, dinitrophenol and protonophore carbonyl cyanide-m-chlorophenyl-hydrazone. The kinetic rearrangement of the glucose transport system in response to altered growth conditions was dependent on de novo protein synthesis.
Karp
H
H
Institute of Molecular and Cell Biology, University of Tartu, Estonia.
Alamäe
T
T
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010843:Pichia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose; D019297:2,4-Dinitrophenol