Close proximity of the tdh, trh and ure genes on the chromosome of Vibrio parahaemolyticus.
The distribution and location of the virulence-factor genes of Vibrio parahaemolyticus, tdh and trh, and the structural gene of urease, ureC, were examined on the genomic DNAs of 115 clinical isolates of V. parahaemolyticus. The majority of strains (81%) had two copies of tdh on the chromosome, and no copies of trh or ure. Southern hybridization with a tdh probe, after pulsed-field gel electrophoresis of Notl-digested genomic DNA of each strain revealed only single bands, suggesting that the two copies of the exist on single Notl fragments in each strain. Of the 115 strains, 7% had the tdh, trh and ure genes on chromosomal DNA. The three genes were also detected on single Notl fragments in these strains. More detailed analysis revealed that the three genes were localized within 40 kb. By long and accurate polymerase chain reactions (LA-PCR) the distance between trh and ure was shown to be less than 8.5 kb. These results reveal a close proximity of the tdh, trh and ure genes on the chromosome of pathogenic V. parahaemolyticus strains.
Lida
Tetsuya
T
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Park
Kwon-Sam
KS
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Suthienkul
Orasa
O
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Kozawa
Junji
J
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Yamaichi
Yoshiharu
Y
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Yamamoto
Koichiro
K
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
Honda
Takeshi
T
Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University3-1 Yamadaoka, Suita, Osaka 565Japan.
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005759:Gastroenteritis / Q000209:etiology; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction; D014510:Urease / Q000235:genetics; D014735:Vibrio Infections / Q000209:etiology; D014736:Vibrio parahaemolyticus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D017931:DNA Primers; D004269:DNA, Bacterial; D006460:Hemolysin Proteins; C088609:thermostable direct hemolysin-related hemolysin protein, Vibrio parahaemolyticus; C068489:thermostable direct hemolysin; D014510:Urease