Vasopressin antagonist improves renal function in a rat model of pneumoperitoneum.
Pneumoperitoneum (PP) is associated with oliguria and increased plasma arginine vasopressin (AVP) levels. This study investigated the role of AVP in the pathogenesis of oliguria due to PP. Anesthetized and ventilated rats (n = 12) were subjected for 1 h to carbon dioxide PP with an intra-abdominal pressure of 8 mmHg or, as control, at 0 mmHg, before the determination of plasma AVP level. Another group of rats (n = 48) subjected to PP or control conditions was pretreated with the AVP V2 receptor antagonist, OPC-31260 (5 mg/kg), or vehicle, and their renal parameters were measured. Glomerular filtration rate (GFR) was determined by inulin clearance in an additional group of rats (n = 12) subjected to PP with or without pretreatment with OPC-31260. Rats subjected to PP had higher plasma AVP levels than did controls (17.3 +/- 8.1 pg/ml vs 1.5 +/- 0. 6 pg/ml, P < 0.05). In rats pretreated with vehicle, PP decreased urine output, excretion of water, and urea nitrogen, leading to reduced serum osmolality and serum sodium levels as well as elevated blood urea nitrogen levels. OPC-31260 pretreatment improved urine output, excretion of water, and urea nitrogen, thereby preventing changes in serum osmolality, serum sodium levels, and blood urea nitrogen levels. OPC-31260 pretreatment did not affect GFR. Results suggest that plasma AVP contributes to the oliguria due to PP. OPC-31260 may be useful in treating the water retention associated with PP.
Dolgor
B
B
Department of Surgery I, Oita Medical University, 1-1 Idaigaoka Hasama-machi, Oita, 879-5593, Japan.
Kitano
S
S
Yoshida
T
T
Bandoh
T
T
Ninomiya
K
K
Matsumoto
T
T
D000818:Animals; D001127:Arginine Vasopressin / Q000097:blood; D001552:Benzazepines / Q000494:pharmacology*; D004231:Diuresis / Q000187:drug effects; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000503:physiopathology*; D008297:Male; D011027:Pneumoperitoneum / Q000097:blood / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014667:Vasopressins / Q000037:antagonists & inhibitors*
D001552:Benzazepines; D014667:Vasopressins; D001127:Arginine Vasopressin; C076251:mozavaptan