Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1.
Human SART-1 (hSART-1) gene encodes a 125 kD protein with a leucine-zipper motif expressed in the nucleus of all proliferating cells, and a 43 kD protein expressed in the cytosol of most epithelial cancers. In this study, two rodent genes (rSART-1 and mSART-1) homologous to hSART-1 were cloned from cDNA libraries of murine brain and a rat tumor cell line, respectively. mSART-1 and rSART-1 were highly homologous to hSART-1 with 86% and 84% identity at the nucleotide level, and 95% and 91% at the protein level, respectively. The leucine zipper domain and two basic amino acid portions that bind DNA, as well as peptide sequences recognized by human cytotoxic T lymphocytes (CTLs), were all conserved in these rodent genes. Nuclear protein homologous to the 125 kD hSART-1(800) protein, but not to the 43 kD cytosol SART-1(259) protein, was detectable with specific antibody in the nuclear fractions of rodent tumor cell lines, and normal rodent fetal liver and testis. These rodent genes should be a novel tool for studies on the biological roles of the SART-1 gene, and also in the construction of animal models of specific immunotherapy using SART-1 gene products.
Sumitomo Pharmaceutical Co., Ltd, Osaka.
M
M
Gotoh
S
S
Shichijo
T
T
Hoshino
Y
Y
Imai
T
T
Imaizumi
Y
Y
Inoue
H
H
Takasu
T
T
Yamaoka
K
K
Itoh
D000595:Amino Acid Sequence; D000818:Animals; D000951:Antigens, Neoplasm; D001483:Base Sequence; D005260:Female; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017411:Ribonucleoproteins, Small Nuclear; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015534:Trans-Activators
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D017411:Ribonucleoproteins, Small Nuclear; C431323:SART1 protein, human; C411898:Sart1 protein, mouse; C498986:Sart1 protein, rat; D015534:Trans-Activators