Interleukin-18/interferon-gamma-inducing factor, a novel cytokine, up-regulates ICAM-1 (CD54) expression in KG-1 cells.
Intercellular adhesion molecule-1 (ICAM-1, CD54) is a membrane glycoprotein and a member of the immunoglobulin superfamily. It plays a central role in cell to cell-mediated immune responses and is a ligand for leukocyte function-associated antigen-1 (LFA-1). We report here that a newly discovered cytokine, interferon-gamma-inducing factor (IGIF) [H. Okamura et al. (1995) Nature 378, 88] recently proposed to be designated as IL-18, selectively up-regulates ICAM-1 expression in KG-1 cells, a human myelomonocytic cell line, in which IL-18 also enhances interferon-gamma production. IL-18 induced heterotypic aggregation between KG-1 and Peer T cells, which was blocked by anti-ICAM-1 and/or LFA-1 antibodies. Anti-interferon-gamma antibody did not block the IL-18-induced up-regulation of ICAM-1 on KG-1 cells. These results thus show that IGIF/IL-18, enhances ICAM-1 expression in KG-1 cells in an interferon-gamma-independent pathway, up-regulates ICAM-1 functions, and that IL-18 might play a potential role in immunoregulation by mediating immune cell infiltration into the tissues.
First Department of Surgery, Okayama University School of Medicine, Shikata, Japan.
H
H
Kohka
T
T
Yoshino
H
H
Iwagaki
I
I
Sakuma
T
T
Tanimoto
Y
Y
Matsuo
M
M
Kurimoto
K
K
Orita
T
T
Akagi
N
N
Tanaka
D002460:Cell Line; D016207:Cytokines / Q000494:pharmacology* / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D018922:HL-60 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics; D039041:Integrin alpha4beta1; D016023:Integrins / Q000235:genetics; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis / Q000235:genetics*; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D020382:Interleukin-18 / Q000494:pharmacology* / Q000502:physiology; D007700:Kinetics; D019041:L-Selectin / Q000235:genetics; D015479:Leukemia, Myelomonocytic, Acute; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics; D016201:Receptors, Lymphocyte Homing / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D016207:Cytokines; D018960:Hyaluronan Receptors; D039041:Integrin alpha4beta1; D016023:Integrins; D007369:Interferon Inducers; D020382:Interleukin-18; D016169:Lymphocyte Function-Associated Antigen-1; D016201:Receptors, Lymphocyte Homing; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D018799:Intercellular Adhesion Molecule-1; D019041:L-Selectin; D007371:Interferon-gamma