Metabolism of cystathionine, N-monoacetylcystathionine, perhydro-1,4-thiazepine-3,5-dicarboxylic acid, and cystathionine ketimine in the liver and kidney of D,L-propargylglycine-treated rats.
Experimental cystathioninuria was induced by injection of D,L-propargylglycine in rats. The novel cystathionine metabolites, N-monoacetylcystathionine (NAc-cysta), perhydro-1,4-thiazepine-3,5-dicarboxylic acid (PHTZDC), and cystathionine ketimine (CK), were identified previously in the urine of patients with cystathioninuria and D,L-propargylglycine-treated rats. In this study, we identified these compounds in the liver and kidney of D,L-propargylglycine-treated rats using liquid chromatography-mass spectrometry with an atmospheric pressure chemical ionization interface system (LC/APCI-MS) and an amino acid analyzer. The metabolism of these compounds in the liver and kidney of D,L-propargylglycine-treated rats was also studied. PHTZDC, NAc-cysta, and CK were accumulated in the rat tissues in proportion to the content of cystathionine after D,L-propargylglycine administration. The concentrations of these compounds in the liver were higher than those in the kidney, and these compounds reached maxima earlier in the liver than in the kidney.
Department of Chemistry, Kochi Medical School, Japan.
J
J
Zhang
M
M
Zhang
D
D
Ma
K
K
Sugahara
H
H
Kodama
D000480:Alkynes / Q000494:pharmacology*; D000592:Amino Acid Metabolism, Inborn Errors / Q000378:metabolism*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003540:Cystathionine / Q000031:analogs & derivatives* / Q000378:metabolism* / Q000652:urine; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013841:Thiazepines / Q000378:metabolism
D000480:Alkynes; D013841:Thiazepines; C029425:S-(L-2-(acetylamino)-2-carboxyethyl)-L-homocysteine; D003540:Cystathionine; C010280:perhydro-1,4-thiazepine-3,5-dicarboxylic acid; C009055:propargylglycine; C038681:cystathionine ketimine; D005998:Glycine