Purification, crystallization and preliminary X-ray diffraction studies of retinal dehydrogenase type II.
One enzyme which catalyzes the last step of the formation of the hormone retinoic acid from vitamin A (retinol) is retinal dehydrogenase type II (Ra1DH2). Ra1DH2, expressed in the Escherichia coli BL21(DE3) strain, was purified and crystallized using ammonium sulfate as a precipitant. These crystals belong to the space group P212121 (a = 108, b = 150, c = 168 A, alpha = beta = gamma = 90 degrees).
Lamb
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AL
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Wang
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X
Napoli
J L
JL
Newcomer
M E
ME
D000445:Aldehyde Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification; D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry* / Q000302:isolation & purification; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification; D050697:Retinal Dehydrogenase
D007527:Isoenzymes; D011993:Recombinant Fusion Proteins; D000445:Aldehyde Oxidoreductases; D050697:Retinal Dehydrogenase