[Loss of heterozygosity microsatellite DNA on chromosome loci 3, 5, 7, 9 and 18 in human pancreatic cancer].
OBJECTIVE
Detecting the loss of heterozygosity in paraffin-embedded pancreatic cancer tissues.


METHODS
Analysing loss of heterzygosity (LOH) of microsatellite DNA on chromosome loci 3,5,7,9 and 18 with PCR-SSLP-silver stain method in pancreatic cancer.


RESULTS
In 45 sporadic pancreatic cancer samples and their paired control tissue, LOH was detected on site for D18S46( 18q21.1,31.0%), D18S474 (18q21.1, 20.0%), D9S176 (9q22-31,20.0%), D3S1234 (3p14.2,17.5%),D 3S1289 (3p21.1, 15.7%), D3S1481 (3p14.2,4.9%), D7S486 (7q22,3.6%), D5S365 (5q32, 3.2%) and D3S587 (3p24-26, 2.6%).


CONCLUSIONS
Different percentages of loss of heterzygosity on specific chromosomal regions were found, and the meaning of the results was discussed. Some key genes may play a role in the pathogenesis of pancreatic cancer.
Li
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W
National Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, 100021 P. R. China. liweimd@public.tpt.tj.cn
Wang
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Wang
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Hao
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Wu
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D000328:Adult; D000368:Aged; D002874:Chromosome Mapping; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D008297:Male; D018895:Microsatellite Repeats; D008875:Middle Aged; D010190:Pancreatic Neoplasms / Q000235:genetics*
D004273:DNA, Neoplasm