Sex steroidal regulation of vessel permeability associated with vessel endothelial cadherin (V-cadherin).
In an attempt to understand the roles of cadherins in the placenta, mRNA expression and biological function of cadherins in 3A(tPA-30-1) cells (derived from human term placenta and transformed by SV40), and in HUV-EC-C cells (derived from the endothelial cells in human umbilical cord) were studied under the influence of sex steroids. Estradiol transiently decreased the endothelial cell barrier properties (ECBP) of HUV-EC-C cells, and progesterone reversed the changes induced by estradiol. However, neither estradiol nor progesterone demonstrated any effect on cell aggregation of either 3A(tPA-30-1) or HUV-EC-C cells. Estradiol transiently decreased the level of V-cadherin and its mRNA in HUV-EC-C cells, and progesterone reversed the level decreased by estradiol. However, neither estradiol nor progesterone demonstrated any effect on the level of E-cadherin mRNA in 3A(tPA-30-1) cells. Therefore, a sex steroidal role for placental development and function related to cadherins seems to focus on the endothelial cells, plausibly via vessel permeability for the utilization of placental products.
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
J
J
Fujimoto
H
H
Sakaguchi
R
R
Hirose
T
T
Tamaya
D015703:Antigens, CD; D015820:Cadherins / Q000235:genetics / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002461:Cell Line, Transformed; D002463:Cell Membrane Permeability / Q000187:drug effects / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D010920:Placenta / Q000166:cytology / Q000502:physiology*; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013539:Simian virus 40 / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects; D014471:Umbilical Veins; D000099152:Cadherin 5
D015703:Antigens, CD; D015820:Cadherins; D004958:Estradiol; D011374:Progesterone; D012333:RNA, Messenger; D000099152:Cadherin 5