[Effects of glycated protein on the expression of plasminogen activator inhibitor-1 of cultured human mesangial cells].
OBJECTIVE
To investigate whether nonenzymatic glycated end products (AGEs) have effects on the expression and bioactivity of plasminogen activator inhibitor-1 (PAI-1), one of the seripin proteinases, which lead to extracellular matrix (ECM) degradation in cultured human mesangial cells.


METHODS
Human mesangial cells (HMC) were cultured. Cell proliferation, fibronectin production, mRNA expression and bioactivity of PAI-1 were determined after exposure to AGE-BSA for 24 hours and 48 hours in vitro.


RESULTS
HMC stimulated by AGE-BSA exhibited inhibition in HMC proliferation, increase in fibronectin production, and PAI-1 bioactivity. These changes were pronounced with prolongation of experimental time. PAI-1 mRNA expression increased significantly at 24 hr (0.45% +/- 0.06% vs 0.65% +/- 0.08%, P < 0.05), however more marked increase of PAI-1 mRNA expression at 48 hr (0.51 +/- 0.08% vs 0.92 +/- 0.10%, P < 0.01).


CONCLUSIONS
Increase of mRNA expression and bioactivity of PAI-1 induced by AGEs decreased ECM degradation and play an important role in the pathogenesis of ECM accumulation and glomerulosclerosis.
Deng
Y
Y
Department of Nephrology, General Hospital of P.L.A., Kidney Center of P.L.A, Beijing.
Chen
X
X
Ye
Y
Y
D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003928:Diabetic Nephropathies / Q000209:etiology; D005353:Fibronectins / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D005920:Glomerular Mesangium / Q000166:cytology* / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000494:pharmacology*; D006801:Humans; D017395:Plasminogen Activator Inhibitor 1 / Q000096:biosynthesis*
D005353:Fibronectins; D017127:Glycation End Products, Advanced; D017395:Plasminogen Activator Inhibitor 1