No significant expression of CYP2E1 in rat liver stellate cells.
The putative role of the ethanol-inducible cytochrome P450(CYP)2E1 in stimulating collagen synthesis by rat liver stellate cells was studied. Analysis of carefully isolated stellate cells revealed that their content of immunoreactive CYP2E1 protein and of CYP2E1 mRNA, as determined by reverse transcription, polymerase chain reaction (RT-PCR), was very low, i.e. only 0-4% of that in hepatocytes. We conclude that it is improbable that such low expression of CYP2E1 in stellate cells would have functional importance.
Alcohol Research Center, National Public Health Institute, Helsinki, Finland.
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Oinonen
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Koivisto
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Lindros
D000818:Animals; D019392:Cytochrome P-450 CYP2E1 / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014158:Transcription, Genetic
D019392:Cytochrome P-450 CYP2E1