[Effect of donor antigen-TCS on survival time of mouse to rat cardiac xenografts].
OBJECTIVE
To induce specific mouse to rat xenograft tolerance.


METHODS
The antibodies affinity chromatography was used to purify mouse major histocompatibility complex antigens (H-2 Ag). H-2 Ag was conjugated with trichosanthin (TCS) by heterobifunctional reagents SPDP and 2-IT. The specific inhibition function of H-2 Ag-TCS to recipient immune cell was Measured. H-2Ag-TCS was used in mouse to rat cardiac xenotransplantation (cuff technique).


RESULTS
The proliferation of recipient immune cell pretreated with conjugate H-2Ag-TCS was inhibited. H-2 Ag-TCS significanlly prolonged the cardiac survival time (6.88 +/- 1.36) compared with CgA group (2.83 +/- 0.75) (P < 0.01).


CONCLUSIONS
H-2 Ag-TCS can induce specific tolerance to xenograft.
Department of Surgery, First Affiliated Hospital, West China University of Medical Sciences, Cheng.
P
P
Lan
W
W
Zhan
J
J
Wang
D000818:Animals; D005260:Female; D006085:Graft Survival / Q000187:drug effects; D006183:H-2 Antigens / Q000494:pharmacology; D016027:Heart Transplantation; D007166:Immunosuppressive Agents / Q000494:pharmacology; D000922:Immunotoxins / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous; D015978:Trichosanthin / Q000494:pharmacology*
D006183:H-2 Antigens; D007166:Immunosuppressive Agents; D000922:Immunotoxins; D015978:Trichosanthin