Influence of ascorbic acid, sodium citrate, and sodium bicarbonate on the uptake of 59Fe-transferrin, 54Mn-transferrin, and 65Zn-transferrin from lactating mouse mammary gland cells.
The effects of ascorbic acid, sodium citrate, and sodium bicarbonate on 59Fe-transferrin, 54Mn-transferrin, and 65Zn-transferrin uptake by the receptors disposed of plasma membrane isolated from lactating mouse mammary gland cells have been investigated. The effect of 10(-2) mol/L ascorbic acid alone and in combination with NaHCO3 on the 59Fe-transferrin uptake is significant and positive. 54Mn-transferrin and 65Zn-transferrin binding to the cell receptors are influenced optimally by 0.5 mol/L sodium bicarbonate. Sodium citrate alone or in combination with other substances always has a negative effect on binding of these three metals. It is suggested that a precise mechanism may exist with large possibilities to rearrange metal uptake and its transport from blood to milk.
Department of Biochemistry, Medical Faculty, Medical University, Sofia, Bulgaria.
D A
DA
Moutafchiev
L M
LM
Sirakov
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D002951:Citrates / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007504:Iron Radioisotopes / Q000378:metabolism / Q000493:pharmacokinetics; D007774:Lactation; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000378:metabolism; D008345:Manganese / Q000378:metabolism / Q000493:pharmacokinetics; D051379:Mice; D011868:Radioisotopes / Q000493:pharmacokinetics; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D000077559:Sodium Citrate; D014168:Transferrin / Q000493:pharmacokinetics*; D015037:Zinc Radioisotopes / Q000378:metabolism / Q000493:pharmacokinetics
D002951:Citrates; D007504:Iron Radioisotopes; D011868:Radioisotopes; D014168:Transferrin; D015037:Zinc Radioisotopes; D000077559:Sodium Citrate; D008345:Manganese; D017693:Sodium Bicarbonate; D001205:Ascorbic Acid