[Substance P in the primary sensory neurons innervating the dental pulp in the guinea pig].
Primary sensory trigeminal neurons supplying the dental pulp of incisors in guinea pigs were labelled by retrograde axonal transport. Using an autometallographic intensification procedure, 48 h after injection of wheat germ agglutinin/colloidal gold in the pulp, gold particles were detected in the cytoplasm of the neurons as black granulations. A morphometric study showed a bimodal repartition of the labelled neurons of the ganglion. By submitting ganglion slices to an anti-substance P immunserum revealed by immunocytochemistry, it could be observed that, among the neurons supplying the dental pulp of incisors, the majority of the largest were substance P immunopositive while the smallest were substance P immunonegative. These observations suggest that there could be at least two different populations of nerve fibres supplying the guinea pig incisor dental pulp. Substance P negative neurons could express different neurotransmitters.
Laboratoire de physiologie de la manducation, université Denis-Diderot, Paris, France.
S
S
Alia
J
J
Azérad
M
M
Janian
G
G
Lévy
B
B
Pollin
D000818:Animals; D001370:Axonal Transport; D006224:Cricetinae; D003593:Cytoplasm / Q000737:chemistry; D003782:Dental Pulp / Q000294:innervation*; D007150:Immunohistochemistry; D008297:Male; D009475:Neurons, Afferent / Q000737:chemistry* / Q000648:ultrastructure; D013373:Substance P / Q000032:analysis*
D013373:Substance P
Substance P dans les neurones sensitifs primaires innervant la pulpe dentaire, chez le cobaye.