Localization and secretion of tissue kallikrein in peptidoglycan-induced enterocolitis in Lewis rats.
The plasma kallikrein-kinin system is a mediator of intestinal inflammation induced by peptidoglycan-polysaccharide from group A streptococci (PG-APS) in rats. In this study we investigated the participation of intestinal tissue kallikrein (ITK). Lewis rats were injected intramurally with PG-APS. ITK was visualized by immunohistochemical staining. Cecal ITK concentration was measured by radioimmunoassay, and gene expression was evaluated by RNase protection assay. Kallikrein-binding protein (KBP) was evaluated in plasma by ELISA. Tissue kallikrein was identified in cecal goblet cells in both control and PG-APS-injected rats and in macrophages forming granulomas in inflamed tissues. Cecal ITK was significantly lower in acute and chronic phases of inflammation and in supernatant from in vitro cultures of inflamed cecum. ITK mRNA levels were not significantly different. Plasma KBP levels were significantly reduced in inflamed rats. The presence of tissue kallikrein in macrophages suggests participation in experimental colitis. The decrease of ITK in the inflamed intestine associated with unchanged mRNA levels suggests ITK release during intestinal inflammation.
Stadnicki
A
A
Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
Chao
J
J
Stadnicka
I
I
Van Tol
E
E
Lin
K F
KF
Li
F
F
Sartor
R B
RB
Colman
R W
RW
D000818:Animals; D002432:Cecum / Q000201:enzymology*; D002478:Cells, Cultured; D004760:Enterocolitis / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006099:Granuloma / Q000201:enzymology / Q000473:pathology; D007150:Immunohistochemistry; D007249:Inflammation; D007413:Intestinal Mucosa / Q000201:enzymology*; D007610:Kallikreins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D008264:Macrophages / Q000201:enzymology; D010457:Peptidoglycan; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D011917:Rats, Inbred Lew; D013297:Streptococcus pyogenes; D020840:Tissue Kallikreins; D014158:Transcription, Genetic
D010457:Peptidoglycan; D012333:RNA, Messenger; D007610:Kallikreins; D020840:Tissue Kallikreins