[Characterization of monoclonal antibodies that recognize the thermolabile toxin of Vibrio cholerae].
The obtention of two monoclonal antibodies which recognize a single epitope present in the subunit B of the thermolabile toxin of Vibrio cholerae and another which shows a cross-reaction with those produced by certain enteropathogenic toxins, is reported. The standardization of a solid phase indirect immunoenzymatic assay (ELISA) for the primary screening and selection of hybrids was performed; in addition, the isotype was determined.
Instituto de Medicina Tropical Pedro Kouri.
M
M
González Garriga
H M
HM
García Sánchez
C
C
Hermida Díaz
R
R
Marcet Sánchez
R
R
Monté Boada
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D002199:Capillary Permeability; D002772:Cholera Toxin / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D014734:Vibrio cholerae
D000911:Antibodies, Monoclonal; D004768:Enterotoxins; D002772:Cholera Toxin
Caracterización de anticuerpos monoclonales que reconocen la toxina termolábil de Vibrio cholerae.