Fructans interact strongly with model membranes.
Bacterial fructans with a high degree of polymerisation cause a very large increase in surface pressure of lipid monolayers at the air-water interface with a broad range of lipids, including phosphatidylethanolamine and several types of phosphatidylcholines. The surface active effect of fructans contrasts strongly with the maximal effects observed for trehalose, sucrose and glucose under comparable conditions (20 and 0.6 mN/m for fructans and the other sugars, respectively). The results demonstrate a profound and specific membrane interaction of the fructans which is probably very different from the effect of the smaller carbohydrates. The fructan concentrations used in this study are within the physiological range observed in fructan-accumulating plants. The suggested water-stress protective effect of fructans may be induced by membrane-fructan interaction which prevent lipid condensation and phase transitions to take place.
Demel
R A
RA
Department Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, Netherlands. r.a.demel@chem.uu.nl
Dorrepaal
E
E
Ebskamp
M J
MJ
Smeekens
J C
JC
de Kruijff
B
B
D001412:Bacillus subtilis / Q000378:metabolism*; D019281:Dimerization; D005630:Fructans / Q000494:pharmacology*; D008051:Lipid Bilayers / Q000378:metabolism*; D008567:Membranes, Artificial
D005630:Fructans; D008051:Lipid Bilayers; D008567:Membranes, Artificial