A flash-annealing technique to improve diffraction limits and lower mosaicity in crystals of glycerol kinase.
A flash-annealing method has been developed that increases the diffraction limits, and simultaneously decreases the mosaicity of glycerol kinase crystals. This technique utilizes brief thawing and rapid freezing cycles of the crystal in the cold nitrogen stream. The effective resolution limits increased almost by 0.8 A, from 3.6 to 2.8 A, and mosaicity values halved.
Yeh
J I
JI
Department of Biological Structure, Biomolecular Structure Center, University of Washington, Box 357742, Seattle, Washington, 98195-7742, USA.
Hol
W G
WG
D003460:Crystallization; D005615:Freezing; D005991:Glycerol Kinase / Q000737:chemistry*; D006358:Hot Temperature; D014961:X-Ray Diffraction
D005991:Glycerol Kinase