Proanthocyanidins from barley bran potentiate retinoic acid-induced granulocytic and sodium butyrate-induced monocytic differentiation of HL60 cells.
Polyphenol extract from barley bran (BPE) induced nitro blue tetrazolium (NBT) reducing activity and alpha-naphthyl butyrate esterase activity in HL60 human myeloid leukemia cells. Because BPE induced the biochemical markers of HL60 cell differentiation, we investigated the effects of proanthocyanidins isolated from BPE on the HL60 cell differentiation of HL60 cells. Prodelphinidin B-3, T1, T2, and T3 induced 26-40% NBT-positive cells and 22-32% alpha-naphthyl butyrate esterase-positive cells. Proanthocyanidins potentiated retinoic acid (all-trans-retinoic acid)-induced granulocytic and sodium butyrate-induced monocytic differentiation in HL60 cells.
Research and Development Center, Hakubaku Co., Ltd., Yamanashi, Japan.
K
K
Tamagawa
S
S
Fukushima
M
M
Kobori
H
H
Shinmoto
T
T
Tsushida
D000872:Anthocyanins / Q000302:isolation & purification / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D020148:Butyric Acid / Q000494:pharmacology*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D006098:Granulocytes / Q000502:physiology; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects*; D006633:Histamine Antagonists / Q000494:pharmacology; D001467:Hordeum / Q000378:metabolism*; D006801:Humans; D007202:Indicators and Reagents / Q000737:chemistry; D009000:Monocytes / Q000502:physiology; D009580:Nitroblue Tetrazolium / Q000737:chemistry; D044945:Proanthocyanidins; D014212:Tretinoin / Q000494:pharmacology*
D000872:Anthocyanins; D000970:Antineoplastic Agents; D000975:Antioxidants; D006633:Histamine Antagonists; D007202:Indicators and Reagents; D044945:Proanthocyanidins; D020148:Butyric Acid; C013221:proanthocyanidin; D009580:Nitroblue Tetrazolium; D014212:Tretinoin; D002265:Carboxylic Ester Hydrolases; C021855:naphthylbutyrate esterase