[Preliminary study of AP-64 cell line (Aedes pseudoscutellaris) for dengue-1 and -2 virus multiplication].
We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.
Morier Díaz
L
L
Instituto de Medicina Tropical Pedro Kouri.
Alemán Miranda
M R
MR
Castillo Alvarez
A
A
Pérez Forcada
V
V
D000330:Aedes / Q000821:virology; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007814:Larva / Q000821:virology; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
Estudio preliminar de la línea celular AP-64 (Aedes pseudoscutellaris) para la multiplicación de los virus dengue-1 y 2.