The ontogeny of the expression of K+ channel-like gene (CHIF) in the rat kidney papilla.
Recently, an IsK-like potassium (K+) channel corticosteroid-induced gene (CHIF) was cloned. A high-K+ diet enhances, while a low-K+ diet decreases the expression of this gene. The major expression of CHIF in the adult rat kidney is in the papilla, where it is constitutive, in contrast to its inducibility by corticosteroids and a low-salt diet in the rat colon. In order to further understand the ontogeny of K+ clearance, we studied the presence of CHIF in the kidney papilla in different stages of rat development. Total RNA from rat kidney papillae of 1- to 3-day pre-labor unborn offspring, 2- to 3-day-old newborns, 10-day-old, 6-week-old, and 43-week-old rats underwent northern hybridization for CHIF and the alpha-subunit of the Na+-K+-ATPase mRNA. Minor expression of CHIF mRNA was found in fetal and newborn rat papillae, while older rats showed an age-related increase in gene expression. The expression of the alpha-sub unit of the Na+-K+-ATPase was not age related. We conclude that CHIF is present in the rat kidney papilla and the expression is related to age. The relative deficiency of CHIF in the newborn may be one of the factors responsible for the reduced K+ clearance in is period.
Nephrology Service, Hadassah Hebrew University Hospital, Jerusalem, Israel.
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Haviv
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Wald
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Garty
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Popovtzer
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active / Q000502:physiology; D015152:Blotting, Northern; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins; D007679:Kidney Medulla / Q000254:growth & development* / Q000378:metabolism*; D008297:Male; D015221:Potassium Channels / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
C094337:Fxyd4 protein, rat; D005938:Glucocorticoids; D047908:Intracellular Signaling Peptides and Proteins; D015221:Potassium Channels; D003907:Dexamethasone; D000254:Sodium-Potassium-Exchanging ATPase