Vascularization of embryonic adrenal gland grafted onto chorioallantoic membrane.
Vascularization and endothelial phenotype expression were analysed in embryonic adrenal tissue grafted onto chorioallantoic membrane (CAM), by means of routine light microscopy and immunocytochemical staining, and of electron microscopy. Adrenal gland tissue from chick or quail embryos (donors) was grafted onto CAMs of chick or quail embryos (host). Vessels of chick origin were discriminated from those of quail origin by monoclonal antibodies, anti-MB1, specific for quail endothelial and haemopoietic cells, and QCPN, which labels quail cell nuclei. Vessels of adrenal type were distinguished from those of CAM-type by their ultrastructural endothelial phenotype - porous in the former and continuous in the latter. The observations carried out 6 days after implantation indicate that the adrenal gland develops and differentiates according to a virtually normal histological pattern. As regards the adrenal and CAM vascularization, the grafting procedure elicits angiogenic events consisting in the formation of peripheral anastomoses between the graft and the CAM original microvasculature and in new-growth of vessels from the CAM into the grafted tissue and vice versa. As to the endothelial phenotype, the ultrastructural results demonstrate that besides its own native vasculature, the adrenal tissue contains vessels with continuous endothelium and the CAM mesenchyme is supplied by adrenal-type, fenestrated vessels.
Bertossi
M
M
Istituto di Anatomia Umana Normale, Istologia ed Embriologia, Facoltà di Medicina, Università di Bari, Italy.
Virgintino
D
D
Coltey
P
P
Errede
M
M
Mancini
L
L
Roncali
L
L
D000311:Adrenal Glands / Q000098:blood supply* / Q000637:transplantation / Q000648:ultrastructure; D000482:Allantois / Q000098:blood supply* / Q000648:ultrastructure; D000818:Animals; D002642:Chick Embryo; D002823:Chorion / Q000098:blood supply* / Q000648:ultrastructure; D003370:Coturnix; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D018919:Neovascularization, Physiologic; D019737:Transplants