Different response to inflammation of the multiple mRNAs of rat N-acetylglucosaminyltransferase I with variable 5'-untranslated sequences.
We found that there are at least five subclasses of N-acetylglucosaminyltransferase I (GnT-I; EC 2.4.1.101) mRNA with different 5'-untranslated regions in rat brain. These five subclasses were also expressed in many tissues with distinct tissue-specific patterns. Moreover, they were regulated differently in response to acute-phase inflammation. The expression of the most abundant subclass of GnT-I mRNA in rat liver decreased 2.5-fold in response to inflammation, concomitantly with a significant decrease in the total amount of GnT-I mRNA. In contrast, one of the minor subclasses of GnT-I mRNA was induced 10-fold by inflammation.
Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Japan.
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Fukada
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Kioka
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Nishiu
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Sakata
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Sakai
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Yamada
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Komano
D000818:Animals; D001921:Brain / Q000201:enzymology; D004926:Escherichia coli; D005091:Exons; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015723:Gene Library; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics*; D008070:Lipopolysaccharides / Q000633:toxicity; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects
D007527:Isoenzymes; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D017351:N-Acetylglucosaminyltransferases; C024850:alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I