Expression of chromosomally inserted bacillus thuringiensis israelensis toxin genes in bacillus sphaericus
Bacillus thuringiensis israelensis delta-endotoxin genes were inserted into transposon Tn917 in plasmid pTV51Ts and cloned into the chromosome of Bacillus sphaericus 2362. Many of the transformants reacted with antibody to the 135-, 128-, 65-, and 28-kDa B.t.israelensis toxin proteins and were approximately 10 times more toxic to A. aegypti larvae than the untransformed host. Some of the transformants differed physiologically and morphologically from the wild-type B. sphaericus. The toxicity of the transformed phenotype was maintained through many transfers in the absence of selective pressure. Copyright 1998 Academic Press.
Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
E
Bar
N
Sandler
M
Makayoto
A
Keynan