[Indirect ultramicroELISA for the detection of total adenovirus antibodies in human serum].
An indirect ultramicroELISA method was normalized for the detection of total antibodies to Adenovirus (AD) (UMELISA AD). The optimum concentration of surface antigen determined was 30 micrograms/mL; serum dilution was of 1:40; and conjugate dilution, of 1:1,000. The UMELISA AD was compared with the complement fixing and indirect immunofluorescence techniques. Results showed accordance by 88% and 96.6%, respectively. The method is valid for the screening of antibodies in seroepidemiological studies as well as for the diagnosis of the infection by paired serum samples.
Savón
C
C
Instituto de Medicina Tropical Pedro Kouri.
Laferté
J
J
Goyenechea
A
A
Valdivia
A
A
Morier
L
L
Tejero
Y
Y
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000914:Antibodies, Viral
UltramicroELISA indirecto para la detección de anticuerpos totales a Adenovirus en suero humano.