Determination of serum tetranectin: technical and clinical evaluation of three sandwich immunoassays.
The performance of two sandwich-type immunoassays for the determination of the tumour marker tetranectin using monoclonal antibodies Hyb 130-13 and 130-14 as catching layer was compared with the performance of a polyclonal assay. Sensitivities were 0.4-0.6 microg/l, and intra- and inter-assay coefficients of variation were < 10% in all assays. One-hundred-and-ten blood donors were examined, and women had higher concentrations of tetranectin in serum than men when measured with monoclonal assays (P < 0.05). In preoperative serum samples from 43 patients with ovarian cancer, tetranectin concentrations were reduced (P < 0.001), and the mean tetranectin concentration decreased with increasing FIGO stage of the patients (P < 0.05). In sera from patients with ovarian cancer, tetranectin concentrations were lower in the polyclonal assay than in the monoclonal assays. This could, hypothetically, be explained by ligand-binding or other conformational changes in tetranectin, influencing the antigenicity of the molecule.
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
A V
AV
Thougaard
C K
CK
Høgdall
S K
SK
Kjaer
J
J
Blaakaer
I
I
Jaliashvili
M
M
Christiansen
D000328:Adult; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D037102:Lectins / Q000032:analysis; D037181:Lectins, C-Type; D008297:Male; D051379:Mice; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood; D011817:Rabbits; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; D001798:Blood Proteins; D037102:Lectins; D037181:Lectins, C-Type; C048648:tetranectin