[The microsatellite instability and loss of heterozygosity in chromosome translocation breakpoint in leukemia].
OBJECTIVE
To investigate the microsatellite instability (MSI) and loss of heterozygosity (LOH) near the chromosome translocation breakpoints of various types of leukemia.


METHODS
PCR amplification of 11 microsatellite loci closely linked to chromosome translocation breakpoints in 30 leukemia patients.


RESULTS
Twenty one in 30 cases of leukemia had MSI in from 1 to 14 microsatellite loci. Eleven in 15 cases of acute leukemia had microsatellite instability (MSI) in more than 1 locus; among them 4 cases had MSI in from 7 to 11 loci. In order of MSI presence rate were D22S315>D9S179>D16S515>D8S559>D12S89. In six cases of chronic myeloid leukemia, 4 cases had MSI in more than 3 loci. Thirteen (2/15) percent of acute leukemia patients had loss of heterozygosity (LOH); the rate was higher than that in other leukemia types.


CONCLUSIONS
The results of MSI and LOH in many cases indicate that there are a lot of allelic alterations in leukemia patients, but those changes have no special relationship with chromosome translocation breakpoints in various leukemia types.
Department of Hematology, The First Affiliated Hospital, Beijing Medical University, Beijing, 100034, P. R. China. cinetnet@public3.bta.net.cn
Y
Y
Fu
P
P
Zhu
W
W
Wang
Y
Y
Liu
H
H
Xue
D019457:Chromosome Breakage; D006801:Humans; D007938:Leukemia / Q000235:genetics*; D019656:Loss of Heterozygosity; D018895:Microsatellite Repeats; D014178:Translocation, Genetic