Automated assay of glycosidases.
The Technicon Basic Auto Analyzer sampler system was modified for simultaneous sampling of glycosidase(s) and substrate-buffer solutions. The inexpensive modification allows performance of automated enzyme analyses and enzyme kinetic studies with minimal consumption of substrate and/or enzyme.
Rackham Arthritis Research Unit, University of Michigan Medical School, Ann Arbor 48109, USA.
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Sarber
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Langnas
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Jourdian
D000118:Acetylglucosaminidase / Q000032:analysis / Q000378:metabolism; D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D006026:Glycoside Hydrolases / Q000032:analysis*; D007700:Kinetics; D008297:Male; D013737:Testis / Q000201:enzymology
D006026:Glycoside Hydrolases; D000118:Acetylglucosaminidase