Crystallization and preliminary X-ray diffraction analysis of cytochrome c' from Rubrivivax gelatinosus at 1.3 A resolution.
Cytochrome c' from the purple non-sulfur phototrophic bacterium Rubrivivax gelatinosus has been crystallized by vapour diffusion at pH 5, 6.3 and 8, in sodium acetate, sodium citrate, and Tris-HCl buffers, respectively. Crystals grown at pH 5 and 6.3 diffract, respectively, to 2.0 A (298 K) and 1.4 A (100 K) using synchrotron radiation. Data up to 1.3 A resolution with 99.8% completeness were collected at 100 K on a crystal grown at pH 8. The space group is P3121 or P3221, and the unit-cell parameters are a = b = 69.63, c = 123.63 A.
Benini
S
S
Institute of Agricultural Chemistry, University of Bologna, Viale Berti Pichat 10 I-40127 Bologna, Italy.
Rypniewski
W R
WR
Wilson
K S
KS
Ciurli
S
S
D003460:Crystallization; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification*; D003627:Data Interpretation, Statistical; D006863:Hydrogen-Ion Concentration; D012244:Rhodospirillaceae / Q000737:chemistry*
D003574:Cytochrome c Group