Protein cross-linking mediated by metalloporphyrins.
A biomimetic protein cross-linking reaction is described which employs oxidatively-activated manganese and iron porphyrins as the reactive species. A wide range of proteins cross-link under these conditions, but only if they are intimately associated in solution. The reaction is rapid, efficient, and will be useful for the suprastructural analysis of multiprotein complexes.
Campbell
L A
LA
Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.
Kodadek
T
T
Brown
K C
KC
D002614:Chelating Agents / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006861:Hydrogen Peroxide; D007501:Iron / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013464:Sulfuric Acids
D002614:Chelating Agents; D003432:Cross-Linking Reagents; D008665:Metalloporphyrins; D011506:Proteins; D013464:Sulfuric Acids; D008345:Manganese; D006861:Hydrogen Peroxide; D007501:Iron; C048813:potassium peroxymonosulfuric acid