The first isolation of Arthroderma benhamiae in Japan.
A clinical isolate of Trichophyton mentagrophytes from rabbit was examined by polymerase chain reaction (PCR) analysis and a mating experiment. The species-specific primers designed from the nucleotide sequences of the chitin synthase 1 (CHS1) gene in the teleomorph of Arthroderma benhamiae amplified a fragment from genomic DNA samples of A. benhamiae and the clinical isolate but not from those of A. simii and A. vanbreuseghemii. On the other hand, the species-specific primers of A. simii and A. vanbreuseghemii did not amplify any fragment from the genomic DNA of the clinical isolates. When the isolate was respectively crossed with (+) or (-) tester strains of A. benhamiae, A. simii and A. vanbreuseghemii, ascospores were produced in the crossing with the A. benhamiae (+) strain. Therefore, the isolate was identified to be A. benhamiae (-), confirming the result of molecular analysis. This is the first report on the isolation of A. benhamiae in Japan.
Kano
R
R
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan. akano@ecc-mail.hongo.ecc.u-tokyo.ac.jp
Nakamura
Y
Y
Yasuda
K
K
Watari
T
T
Watanabe
S
S
Takahashi
H
H
Tsujimoto
H
H
Hasegawa
A
A
D000818:Animals; D003883:Arthrodermataceae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D002687:Chitin Synthase / Q000235:genetics; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary*; D005800:Genes, Fungal; D006801:Humans; D007564:Japan; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits / Q000382:microbiology*; D013045:Species Specificity
D017931:DNA Primers; D004271:DNA, Fungal; D002687:Chitin Synthase