Characterization of the gene for human EMMPRIN, a tumor cell surface inducer of matrix metalloproteinases.
EMMPRIN (extracellular matrix metalloproteinase inducer) also known as CD147 and basigin, is a member of the immunoglobulin family that is present on the surface of tumor cells and stimulates nearby fibroblasts to synthesize matrix metalloproteinases. Using our EMMPRIN cDNA, we have isolated a cosmid clone that contains the human EMMPRIN gene. S1 analysis with a fragment of the gene clone and primer extension of the mRNA was performed to determine the transcription start site. PCR and sequence analysis have defined the exon/intron organization of the gene and show that it is highly conserved with the mouse EMMPRIN/basigin gene. About 950 bases of the 5'-flanking region were examined for transcription factor consensus binding sites, locating three SP1 sites and two AP2 sites. The transcription start site was found to be located in a CpG island. Elements in the proximal promoter region were conserved in the human and mouse genes.
Guo
H
H
Department of Anatomy and Cellular Biology, Tufts Medical School, Boston, Massachusetts, USA.
Majmudar
G
G
Jensen
T C
TC
Biswas
C
C
Toole
B P
BP
Gordon
M K
MK
D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D001483:Base Sequence; D051926:Basigin; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000302:isolation & purification; D004790:Enzyme Induction; D005091:Exons; D006801:Humans; D007438:Introns; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D008666:Metalloendopeptidases / Q000096:biosynthesis*; D051379:Mice; D008969:Molecular Sequence Data; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D015703:Antigens, CD; D000951:Antigens, Neoplasm; C497886:BSG protein, human; D014408:Biomarkers, Tumor; C497887:Bsg protein, mouse; D018387:Codon, Initiator; D008562:Membrane Glycoproteins; D051926:Basigin; D008666:Metalloendopeptidases