Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control.
Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.
WFM Wasserforschung Mainz GmbH, Mainz, Germany. 0613112-6430@t-online.de
E
E
Frahm
I
I
Heiber
S
S
Hoffmann
C
C
Koob
H
H
Meier
W
W
Ludwig
R
R
Amann
K H
KH
Schleifer
U
U
Obst
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D016983:Enterococcus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011786:Quality Control; D012329:RNA, Bacterial / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics*; D013045:Species Specificity; D014871:Water Microbiology; D014881:Water Supply / Q000592:standards*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D015345:Oligonucleotide Probes; D012329:RNA, Bacterial; D012338:RNA, Ribosomal, 23S