Growth modulating effects of chlorinated oleic acid in cell cultures.
Chlorinated fatty acids represent a major fraction of extractable, organically bound chlorine in fish. After dietary intake such fatty acids may be transferred from the mother to the foetus through the placenta, and via breast milk to the child. In the present work we have studied the effect of chlorinated oleic acid on the growth of three widely differing types of cells in culture. Chlorinated oleic acid inhibited growth of Human Microvascular Endothelial Cells (HMVEC), Immortilized Human Kidney Epithelial (IHKE) cells, and human Hepatoma cells (HepG2). The order of potency was: HMVEC > IHKE > HepG2. Vitamin E counteracted the inhibitory effect of chlorinated oleic acid on HepG2 cells, but did not significantly affect the fatty acid effect on HMVEC or IHKE. Defatted serum albumin stimulated the growth of HMVEC and IHKE. With HMVEC there was no major interaction between the effect of albumin and chlorinated oleic acid on cell growth. In contrast, with IHKE albumin at low concentration abolished the growth inhibiting effect of chlorinated oleic acid and appreciably counteracted growth inhibition by the fatty acid of HepG2. We conclude that the growth modulation by chlorinated oleic acid and its interaction with vitamin E and albumin are cell specific.
Department of Preventive Medicine, University of Oslo, Norway.
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Høstmark
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Lystad
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Jebens
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Skramstad
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Frøyen
D000418:Albumins / Q000494:pharmacology; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology*; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004730:Endothelium, Vascular / Q000166:cytology* / Q000187:drug effects; D004847:Epithelial Cells; D006801:Humans; D007668:Kidney / Q000166:cytology* / Q000187:drug effects; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology*; D013229:Stearic Acids / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
C114375:9,10-dichlorostearic acid; D000418:Albumins; D013229:Stearic Acids; D014810:Vitamin E