Characterizing cortical neuron injury with Fluoro-Jade labeling after a neurotoxic regimen of methamphetamine.
We used Fluoro-Jade, a recently-developed fluorescent indicator of neuronal damage, to identify neurons injured 1-21 days after repeated injections of methamphetamine (m-AMPH) or saline. The m-AMPH-treated rats showed Fluoro-Jade positive neurons in parietal cortex (layers III and IV) and had less striatal tyrosine hydroxylase immunoreactivity than did saline-injected controls. Fluoro-Jade positive neurons were greatest in number 3 days post-treatment; some fluorescent neurons displayed bud-like surface protrusions. These observations support the hypothesis that certain neocortical neurons degenerate after m-AMPH.
Eisch
A J
AJ
Department of Psychobiology, University of California, Irvine 92697-4550, USA.
Schmued
L C
LC
Marshall
J F
JF
D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D005456:Fluorescent Dyes; D008297:Male; D008694:Methamphetamine / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D009498:Neurotoxins / Q000633:toxicity; D010296:Parietal Lobe / Q000187:drug effects / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D005456:Fluorescent Dyes; D009498:Neurotoxins; D008694:Methamphetamine; D014446:Tyrosine 3-Monooxygenase