Effects of selenium supplementation on virus-induced inflammatory heart disease.
The effects of 10 wk of selenium (Se) supplementation (5 ppm) in drinking water on immune responses and resistance to a myocarditic Coxsackie virus B3 (CB3) infection were studied in female Balb/c mice. Se supplementation reduced CB3-induced mortality: at day 14 postinoculation, survival was 58% in the Se-treated group as compared to 25% in the untreated group. Whole-blood glutathione peroxidase (GSH-Px) activity was elevated by 68% (p < 0.001) and Se content in the liver by 24% (p < 0.001). Red (RBC) and white blood cell (WBC) counts, as well as the number of cells in the spleen and thymus, were unaffected. The cellular counts of T-lymphocytes (CD4+, CD8+) and natural killer (NK+) cells in the blood were not affected. However, the CD4+/CD8+ ratio (5.2) tended to increase after Se supplementation (5.9). The spleen lymphoproliferative response to T- and B-cell mitogens were increased by 9 and 43%, respectively (ns), in the Se-supplemented group. The total NK cell activity in blood and spleen showed minor increases, but when the activity in the blood was expressed per cell, the increase amounted to 35% (ns) with Se supplementation. The inflammatory and necrotic lesions in the ventricular myocardium at 7 and 14 d postinoculation were not significantly reduced by Se treatment, probably owing to the increased survival with Se even of mice with the most pronounced heart damage; comparable untreated mice were estimated to have died at day 14. Results indicate that modest doses of Se can improve immune function, which may increase the general resistance to this viral infection.
Toxicology Division, National Food Administration, Uppsala, Sweden.
N G
NG
Ilbäck
J
J
Fohlman
G
G
Friman
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003384:Coxsackievirus Infections / Q000188:drug therapy* / Q000473:pathology; D029822:Enterovirus B, Human / Q000472:pathogenicity; D004906:Erythrocyte Count / Q000187:drug effects; D005260:Female; D007694:Killer Cells, Natural / Q000187:drug effects; D007958:Leukocyte Count / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009205:Myocarditis / Q000188:drug therapy* / Q000473:pathology / Q000821:virology*; D009206:Myocardium / Q000473:pathology; D012643:Selenium / Q000494:pharmacology*; D015996:Survival Rate; D013601:T-Lymphocytes / Q000187:drug effects
D012643:Selenium