The genome-linked protein (VPg) of southern bean mosaic virus is encoded by the ORF2.
The sequence of the 20 N-terminal amino acids of the viral protein (VPg) which is covalently attached to the genomic RNA of the bean strain of Southern bean mosaic virus (SBMV-B) has been determined. The obtained VPg sequence mapped to position 327 to 346 of the SBMV-B ORF2 product, downstream of the putative protease domain and in front of the RNA-dependent RNA polymerase. Thus indicating that the sobemovirus genomic arrangement is similar to that of subgroup II luteoviruses. Comparison with other viral sequences revealed a high similarity with the sequence of the ORF2-product of the cowpea strain of SBMV (SBMV-C). No significant similarities were detected with amino acid sequences derived of other sobemoviruses or non-related viruses.
van der Wilk
F
F
Department of Virology, DLO Research Institute for Plant Protection (IPO-DLO), Wageningen, The Netherlands. F.vanderWilk@IPO.DLO.NL
Verbeek
M
M
Dullemans
A
A
van den Heuvel
J
J
D000595:Amino Acid Sequence; D007887:Fabaceae / Q000821:virology*; D005814:Genes, Viral / Q000235:genetics; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000737:chemistry / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D010946:Plants, Medicinal; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D014758:Viral Core Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015678:Viral Structural Proteins / Q000235:genetics
D012367:RNA, Viral; D014758:Viral Core Proteins; D015678:Viral Structural Proteins