[Comparison of the synonymous and non-synonymous substitution rates in various regions of the neuraminidase and hemagglutinin genes of the influenza A virus].
A new, statistically justified approach was used to estimate the synonymous and non-synonymous substitution rates in several antigenic variants of influenza-virus surface proteins. The rates were compared for antigenic and nonantigenic regions of neuraminidase and hemagglutinin, as well as for neuraminidase surface and internal amino acids identified by X-ray analysis. For neuraminidase, the estimation was performed for the first time. The non-synonymous substitution rate was shown to be significantly higher in antigenic than in nonantigenic sites. However, neither subsample of antigenic sites displayed a fixation rate of non-synonymous substitutions higher than that of synonymous substitutions, which would confirm the effect of positive selection on these sites and argue against a neutral evolution character. Specific features of methods used to estimate the substitution fixation rates and problems in their interpretation are discussed.
Makarova
K S
KS
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Wulf
Iu I
IuI
Tereza
E P
EP
Ratner
V A
VA
D019943:Amino Acid Substitution; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000737:chemistry*; D005814:Genes, Viral; D006388:Hemagglutinins / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics*
D000956:Antigens, Viral; D006388:Hemagglutinins; D009439:Neuraminidase
Sravnenie skorosti nakopleniia sinonimicheskikh i nesinonimicheskikh zamen dlia razlichnykh raĭonov genov neĭraminidazy i gemaggliutinina virusa grippa A.