T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis.
Patients with early-onset periodontitis (EOP) are susceptible to infection with periodontopathic bacteria, such as Porphyromonas gingivalis. Ag53, 53-kDa outer membrane protein of P. gingivalis, evokes strong humoral immune responses in EOP patients. In a first step to clarify how host immune cells recognize Ag53, we established Ag53-specific short-term T cell lines from 22 subjects including 6 EOP patients and 16 healthy donors, using overlapping peptides based on Ag53 amino acid sequences. All T cell lines from active EOP patients recognized a common region (p141-181, especially p141-161) on Ag53, while those from healthy donors showed heterogeneous specificity. p141-181 was not recognized by T cell lines established from EOP patients following therapy. A monoclonal antibody to HLA-DRB 1 inhibited Ag53-induced proliferation of most of the T cell lines. Our observations suggest that, although antigen-presenting molecules are common in EOP patients and in healthy individuals, p141-161 includes a major T cell epitope(s) on Ag53 for active EOP patients but not for healthy individuals or inactive EOP patients.
Ohyama
H
H
Department of Periodontology and Endodontology, Okayama University Dental School, Japan.
Matsushita
S
S
Kato
N
N
Nishimura
F
F
Oyaizu
K
K
Kokeguchi
S
S
Kurihara
H
H
Takashiba
S
S
Nishimura
Y
Y
Murayama
Y
Y
D000328:Adult; D017668:Age of Onset; D000595:Amino Acid Sequence; D017951:Antigen Presentation; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D002460:Cell Line; D018604:Epitope Mapping; D005260:Female; D006680:HLA Antigens / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D010518:Periodontitis / Q000276:immunology*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
C115755:53 kDa OMP protein, Porphyromonas; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006680:HLA Antigens; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments