Using atomic force microscopy to investigate patch-clamped nuclear membrane.
Nuclear patch clamp is an emerging research field that aims to disclose the electrical phenomena underlying macromolecular transport across the nuclear envelope (NE), its properties as an ion barrier and its function as an intracellular calcium store. The authors combined the patch clamp technique with atomic force microscopy (AFM) to investigate the structure-function relationship of NE. In principle, patch clamp currents, recorded from the NE can indicate the activity of the nuclear pore complexes (NPCs) and/or of ion channels in the two biomembranes that compose the NE. However, the role of the NPCs is still nuclear because the observed NE current in patch clamp experiments is lower than expected from the known density of the NPCs. Therefore, AFM was applied to link patch clamp currents to structure. The membrane patch was excised from the nuclear envelope and, after electrical evaluation, transferred from the patch pipette to a substrate. We could identify the native nuclear membrane patches with AFM at a lateral and a vertical resolution of 3 nm and 0.1 nm, respectively. It was shown that complete NE together with NPCs can be excised from the nucleus after their functional identification in patch clamp experiments. However, we also show that membranes of the endoplasmic reticulum can contaminate the tip of the patch pipette during nuclear patch clamp experiments. This possibility must be considered carefully in nuclear patch clamp experiments.
Physiologisches Institut, Würzburg, Germany.
T
T
Danker
M
M
Mazzanti
R
R
Tonini
A
A
Rakowska
H
H
Oberleithner
D000818:Animals; D002467:Cell Nucleus / Q000502:physiology* / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force / Q000379:methods*; D009685:Nuclear Envelope / Q000502:physiology* / Q000648:ultrastructure*; D018408:Patch-Clamp Techniques; D013329:Structure-Activity Relationship; D014982:Xenopus laevis