Establishment of monoclonal antibody against human Apo B-48 and measurement of Apo B-48 in serum by ELISA method.
The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is translated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide representing human Apo B-48 C-terminal sequence as immunogen, we established a monoclonal antibody, B48-151, against human Apo B-48. The specific reactivity for Apo B-48 of this monoclonal antibody was confirmed using Western blot analysis of human plasma in fractions isolated as chylomicron and VLDL. Then, we developed a simple sandwich ELISA method for the detection of human Apo B-48 in serum by combining B48-151 as capturing antibody and HRP-conjugated-polyclonal antibodies for Apo B as signaling antibody. The established sandwich ELISA constitutes a simple method to monitorApo B-48 level in chylomicrons and chylomicron remnants in human serum.
Fujirebio, Inc. Diagnostic Research Laboratories, Hachioji, Tokyo, Japan.
Y
Y
Uchida
Y
Y
Kurano
S
S
Ito
D000328:Adult; D000911:Antibodies, Monoclonal; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B / Q000097:blood* / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002914:Chylomicrons / Q000097:blood / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005215:Fasting / Q000097:blood; D005260:Female; D006801:Humans; D008297:Male; D019518:Postprandial Period
D000911:Antibodies, Monoclonal; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; C116189:B48-151 monoclonal antibody; D002914:Chylomicrons