Rat strain minimally influences anesthetic and convulsant requirements of inhaled compounds in rats.
We assessed the effect of rat strain on susceptibility to anesthesia and convulsions produced by inhaled compounds. We determined the minimum alveolar anesthetic concentration (MAC) of desflurane and nitrous oxide, and the convulsive 50% effective dose (ED50) of 1,2-dichlorohexafluorocyclobutane, flurothyl, and difluoromethyl-1-chlorotetrafluoroethyl ether in five strains (three inbred [Long Evans, Sprague-Dawley, and Wistar] and two outbred [Fischer and Brown Norway]). Strain had slight effects on anesthetic potency, the strains with the highest MAC values (Long Evans and Brown Norway) having values < or =28% greater than the strains with the lowest values (Sprague Dawley and Wistar). MAC for nitrous oxide correlated directly with MAC for desflurane as a function of strain. MAC for either desflurane or nitrous oxide correlated inversely with the convulsive ED50 of 1,2-dichlorohexafluorocyclobutane, but correlated poorly (and directly) with the convulsive ED50 of the remaining compounds. Convulsivity varied little as a function of strain (greatest difference 21%) and did not vary consistently as a function of strain. No consistent difference was seen between inbred versus outbred strains.


CONCLUSIONS
Rat strain has a minimal effect on the potency of inhaled anesthetics or the convulsant activity of inhaled compounds. It seems that the sites acted on by inhaled compounds to produce anesthesia and convulsions are conserved across common rat strains.
Department of Anesthesia, University of California-San Francisco, 94143-0464, USA.
D
D
Gong
Z
Z
Fang
P
P
Ionescu
M J
MJ
Laster
R C
RC
Terrell
E I
EI
Eger
D000758:Anesthesia; D018685:Anesthetics, Inhalation / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D000077335:Desflurane; D005019:Ethyl Ethers / Q000494:pharmacology / Q000633:toxicity; D007530:Isoflurane / Q000031:analogs & derivatives / Q000494:pharmacology / Q000633:toxicity; D009609:Nitrous Oxide / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D011919:Rats, Inbred Strains; D012640:Seizures / Q000139:chemically induced*
D018685:Anesthetics, Inhalation; D005019:Ethyl Ethers; D000077335:Desflurane; D007530:Isoflurane; D009609:Nitrous Oxide