Differential adhesiveness between blood and marrow leukemic cells having similar pattern of VLA adhesion molecule expression.
Functional adhesion of blood and marrow leukemic cells from 14 acute myeloid leukemia patients presenting with hyperleukocytosis was evaluated by performing cytoadhesion assays on purified (extracellular matrix proteins) and non-purified supports (MRC5 fibroblastic cell line). Results, in 30-min chromium release assay, show a mean +/- S.D. adhesion to fibronectin, collagen, and laminin respectively of 30 +/- 17%, 20 +/- 13%, 25 +/- 17% for blood leukemic cells and 18 +/- 11%, 11 +/- 10%, 11 +/- 8% for marrow leukemic cells. These differences between blood and marrow cells were statistically significant (respectively P = 0.005, P = 0.01 and P = 0.002), while no difference was noted regarding adhesion to non-purified supports. The higher adhesion of blood blast cells to purified supports was observed regardless of CD34 expression. No significant difference was observed in the expression of cell surface VLA-molecules (CD29, CD49b, CD49d, CD49e, CD49f) between blood and marrow blast cells. The addition of GM-CSF or G-CSF induced increased adhesion of marrow blasts and decreased adhesion of blood blasts leading to a loss of the difference between blood and marrow cells. In a 60-min chromium release assay, marrow blasts adhered even more than blood leukemic cells to fibronectin. In contrast, marrow blasts from 'aleukemic' acute myeloid leukemia patients did not show any modification regarding their adhesion to extracellular matrix proteins when co-cultured with growth factors.
Hôpital Edouard Herriot, UFR Alexis Carrel, INSERM Unité 453, Centre Léon Bérard, Lyon, France.
X
X
Thomas
B
B
Anglaret
M
M
Bailly
O
O
Maritaz
J P
JP
Magaud
E
E
Archimbaud
D015703:Antigens, CD / Q000097:blood*; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules / Q000097:blood*; D002460:Cell Line; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism / Q000473:pathology*; D016029:Receptors, Very Late Antigen / Q000097:blood*; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; D016029:Receptors, Very Late Antigen