IL-5-producing T cells that induce airway eosinophilia and hyperresponsiveness are suppressed by dexamethasone and cyclosporin A in mice.
We have recently demonstrated that airway eosinophilic inflammation can be transferred to unprimed mice by infusion of IL-5-producing T cell clones. In this study, we investigated the effects of dexamethasone and cyclosporin A on the airway eosinophilic inflammation in mice transferred with T cell clones. An ovalbumin-reactive T cell clone, KW29, produced IL-5 as well as IL-2 and IL-4 upon stimulation with relevant antigen. Dexamethasone and cyclosporin A dose-dependently suppressed the production of these cytokines in vitro. The number of eosinophils recovered in the bronchoalveolar lavage fluid and the airway responsiveness to acetylcholine were increased in KW29-transferred mice after antigen provocation. Both responses were dose-dependently suppressed by the administration of dexamethasone or cyclosporin A in vivo. We concluded that airway eosinophilic inflammation can be controlled by agents capable of downregulating IL-5 production in T cells.
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
K
K
Wada
O
O
Kaminuma
A
A
Mori
A
A
Nakata
K
K
Ogawa
H
H
Kikkawa
K
K
Ikezawa
M
M
Suko
H
H
Okudaira
D019264:Adoptive Transfer; D000818:Animals; D016535:Bronchial Hyperreactivity / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D002999:Clone Cells; D016572:Cyclosporine / Q000494:pharmacology*; D003907:Dexamethasone / Q000494:pharmacology*; D004802:Eosinophilia / Q000209:etiology* / Q000276:immunology / Q000517:prevention & control; D007166:Immunosuppressive Agents / Q000494:pharmacology; D015848:Interleukin-5 / Q000096:biosynthesis*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D012140:Respiratory Tract Diseases / Q000209:etiology / Q000276:immunology / Q000517:prevention & control
D007166:Immunosuppressive Agents; D015848:Interleukin-5; D003907:Dexamethasone; D016572:Cyclosporine; D010047:Ovalbumin