Growth factor protection against cytokine-induced apoptosis in neonatal rat islets of Langerhans: role of Fas.
Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or insulin-like growth factor-1 (10(-8)M) gave only partial protection against cell killing, but prevented the Fas-mediated component. In the absence of cytokine treatment, Fas-mediated killing was not observed.
Department of Biochemistry, School of Biological Sciences, University of Sussex, Brighton, UK. moirah@sussex.ac.uk
M
M
Harrison
A M
AM
Dunger
S
S
Berg
J
J
Mabley
N
N
John
M H
MH
Green
I C
IC
Green
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D007371:Interferon-gamma / Q000494:pharmacology; D007515:Islets of Langerhans / Q000276:immunology / Q000473:pathology*; D051381:Rats; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019014:fas Receptor / Q000276:immunology*
D007004:Hypoglycemic Agents; D007328:Insulin; D014409:Tumor Necrosis Factor-alpha; D019014:fas Receptor; D007334:Insulin-Like Growth Factor I; D007371:Interferon-gamma