Capillary electrophoretic study of the complex formation between DNA and cationic surfactants.
Due to the growing interest in the use of cationic surfactants for the construction of liposomal genetic delivery systems, the study of complex formation between DNA and quaternary ammonium detergents is of fundamental importance. In this context, we undertook the study of this complex formation using capillary zone electrophoresis (CZE) with suppressed electroosmotic flow, a technique that allowed us to both monitor the change in mobility of DNA as a function of added surfactant in a precise and reproducible manner and evaluate the potential of CZE to reflect the change in hydrodynamic friction upon binding. Nevertheless, CZE must be applied with caution for binding studies where strong cooperativity occurs, because of the presence of peak splitting at concentrations close to the half-point of binding. Also, a comparison between this experiment and Manning's polyelectrolyte transport properties theory on one hand and Tirado and Garcia de la Torre expression for hydrodynamic friction of rod-like molecules on the other hand is given.
Jacquier
J C
JC
Irish Centre for Colloid Science and Biomaterials, Department of Chemistry, University College Dublin, Ireland.
Gorelov
A V
AV
McLoughlin
D M
DM
Dawson
K A
KA
D000818:Animals; D002412:Cations; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D013501:Surface-Active Agents / Q000737:chemistry*
D002412:Cations; D013501:Surface-Active Agents; D004247:DNA