A protein (M9) associated with monoclonal antibody-mediated agglutination of Mycoplasma gallisepticum is a member of the pMGA family.
A 62-kDa cell surface antigen (M9) of Mycoplasma gallisepticum PG31 that mediates antibody-induced agglutination of the organism was purified and subjected to N-terminal amino-acid sequencing. A 999-bp region of the cDNA encoding the M9 protein was generated by reverse transcription-PCR, and its nucleotide sequence was determined. PCR primers based on this sequence were used to screen a genomic DNA library of PG31. A full-length M9 protein-encoding gene was isolated and sequenced, revealing 96% nucleotide identity with the pMGA1.1 gene of M. gallisepticum S6. Sequence analyses of the M9 gene and flanking open reading frames that encode other pMGA family members suggest that a tandemly repeated GAA sequence may influence pMGA gene expression.
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849, USA.
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L
Liu
D M
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Payne
V L
VL
van Santen
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Dybvig
V S
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Panangala
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D003001:Cloning, Molecular; D006384:Hemagglutination / Q000276:immunology*; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000276:immunology*; D009174:Mycoplasma / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D001426:Bacterial Proteins; C079720:M9 protein, Mycoplasma gallisepticum