Caspase 7-induced cleavage of kinectin in apoptotic cells.
Kinectin has been characterized as the first known receptor for the molecular motor kinesin, which is critically involved in microtubule-based vesicle transport and membrane trafficking. Here we identify kinectin as a target for caspase-mediated proteolysis during apoptosis. Treatment of cells with diverse apoptotic stimuli including TNF, anti-Fas, anticancer drugs, gamma-radiation or ceramide leads to rapid proteolytic cleavage of the 160-kDa form of kinectin to a 120-kDa fragment. Evidence is provided that kinectin cleavage is mediated by caspase 7.
Institute of Immunology, Christian-Albrechts University of Kiel, Germany.
T
T
Machleidt
P
P
Geller
R
R
Schwandner
G
G
Scherer
M
M
Krönke
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology; D000906:Antibodies / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D053179:Caspase 7; D020169:Caspases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D002474:Cell-Free System; D002518:Ceramides / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D005047:Etoposide / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D019169:Jurkat Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008565:Membrane Proteins; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D019014:fas Receptor / Q000276:immunology / Q000378:metabolism
D000590:Amino Acid Chloromethyl Ketones; D000906:Antibodies; D002518:Ceramides; D015853:Cysteine Proteinase Inhibitors; C088547:KTN1 protein, human; D008565:Membrane Proteins; C098738:N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D019014:fas Receptor; D005047:Etoposide; C505520:CASP7 protein, human; D053179:Caspase 7; D020169:Caspases; D002945:Cisplatin; D003630:Daunorubicin