Human parvovirus B19: prevalence of viral DNA in volunteer blood donors and clinical outcomes of transfusion recipients.
OBJECTIVE
Blood donor units are not screened for human parvovirus B19 (B19) even though it can be acquired via blood products. We estimated the prevalence of B19 in a US volunteer blood donor population and determined the clinical outcomes of transfusion recipients.


METHODS
Donor units were screened for B19 DNA by PCR, and positive units analyzed by EIA for B19 Ig. Unit usage was determined and recipient chart review conducted.


RESULTS
B19 DNA was detected in 11/9, 568 allogeneic units (0.1%), of which 3 had no measurable B19 Ig. One individual developed anemia consistent with B19 infection after receiving a DNA+ unit lacking B19 Ig.


CONCLUSIONS
The apparent low incidence of disease in patients transfused with B19 DNA+ components may be due to coexistence of neutralizing antibodies in donors and/or recipients.
Jordan
J
J
Department of Pathology, Magee-Women's Research Institute, University of Pittsburgh, PA 15213, USA. jordanja+@pitt.edu
Tiangco
B
B
Kiss
J
J
Koch
W
W
D000740:Anemia / Q000209:etiology; D000906:Antibodies / Q000097:blood; D001782:Blood Donors / Q000706:statistics & numerical data*; D004279:DNA, Viral / Q000097:blood; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000150:complications / Q000453:epidemiology / Q000209:etiology; D016732:Parvovirus B19, Human / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015995:Prevalence; D065227:Transfusion Reaction; D014481:United States / Q000453:epidemiology; D014759:Viral Envelope Proteins / Q000276:immunology
D000906:Antibodies; D004279:DNA, Viral; D014759:Viral Envelope Proteins