Determination of 2-n-propylquinoline in mouse plasma and liver by high-performance liquid chromatography.
A high-performance liquid chromatographic method was developed for the specific determination of 2-n-propylquinoline, a new anti-leishmaniasis drug, in plasma and liver homogenates of mice. 2-n-Propylquinoline was extracted with methyl-tert.-butyl ether with quinoline as internal standard. Separation was carried out using a Nucleosil C18 column. The mobile phase consisted of methanol-0.005 M ammonium acetate buffer (60:40) at pH 5.5 and 8 for plasma and liver homogenates, respectively. Detection was monitored at 233 nm. The method was validated and shown to be accurate and precise for plasma and liver homogenates. Extraction yield was 96% in plasma and 81% in liver homogenates. This method was used to determine the pharmacokinetic profile of 2-n-propylquinoline following oral administration to mice.
Service de Pharmacie Clinique et des Biomatériaux, G H X Bichat-Cl Bernard, Paris, France.
M
M
Iglarz
B
B
Baune
J C
JC
Gantier
R
R
Hocquemiller
R
R
Farinotti
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D008099:Liver / Q000378:metabolism*; D051379:Mice; D011804:Quinolines / Q000097:blood / Q000493:pharmacokinetics*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
C116019:2-propylquinoline; D011804:Quinolines