The radioprotective effect of a new aminothiol (20-PRA).
We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of 60Co gamma-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58%) was seen 24 h after irradiation, followed by a decrease at 48 h (11%). At the high radiation dose (20 Gy) a low protective effect was also seen (35%). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 10(5)-10(6) times higher than that of the aminothiol WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane) whose protective effect is in the 0.1 to 1.0 mM range.
Departamento de Farmacologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.
M F
MF
Dolabela
M T
MT
Pereira
C E
CE
Salas
G M
GM
Steffani
D L
DL
Nelson
D
D
Piló-Veloso
M T
MT
Lopes
D000818:Animals; D006801:Humans; D008624:Mercaptoethylamines / Q000494:pharmacology*; D011437:Propylamines / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
C116676:2-N-propylamine-cyclo-hexanethiol; D008624:Mercaptoethylamines; D011437:Propylamines; D011837:Radiation-Protective Agents; D013438:Sulfhydryl Compounds; C020174:N-(2-mercaptoethyl)-1,3-diaminopropane