Crystallization and preliminary X-ray studies of allophycocyanin from red alga Porphyra yezoensis.
Allophycocyanin from red alga Porphyra yezoensis has been crystallized in three crystal forms. Form 1 crystals with space group P4132 or P4332 and cell parameters a = 286 A, alpha = 90 degrees were obtained by using isopropanol as precipitant. These crystals did not diffract beyond 5.8 A and could not be used in structure analysis. Form 2 crystals were obtained when crystallization conditions were slightly changed by adding 0.2 M magnesium chloride. The space group of form 2 was not determined because it was difficult to get large single crystals. Form 3 crystals were obtained by using ammonium sulfate as precipitant. The space group of form 3 is R32 with cell dimensions a = b = 105.3, c = 189.4 A and one alpha beta unit in the asymmetric unit. These crystals diffract up to 2.06 A resolution and are suitable for structure determination by molecular-replacement methods.
Liu
J Y
JY
State Key Laboratory, of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.
Zhang
J P
JP
Wan
Z L
ZL
Liang
D C
DC
Zhang
J P
JP
Wu
H J
HJ
D003460:Crystallization; D018360:Crystallography, X-Ray; D010798:Phycocyanin / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D000461:Rhodophyta / Q000737:chemistry*
D010940:Plant Proteins; C002497:allophycocyanin; D010798:Phycocyanin