Evaluation of a new antigen capture ELISA for detection and characterization of platelet alloantibodies.
The monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay is considered as a possible reference method for the detection of maternal alloantibodies when a foetomaternal alloimmunization is suspected. However, this method is tedious. In this study, we have compared the MAIPA results of 54 samples of mothers with (n=34) or without (n=20) alloantibodies with those obtained with a new antigen capture ELISA. Using the cutoff of 2.0 given by the manufacturer, the new kit had a sensitivity of 88.2% (95% CI 72.6-96.7) and a specificity of 100% (95% CI 98.0-100). From a receiver-operating characteristic curve analysis, the more appropriate cutoff for a screening assay would be 1.6, which gives a sensitivity of 100% (95% CI 89.7-100) with a specificity of 94.0% (95% CI 89.4-96.9). In conclusion, this new simple assay appears promising and could be used, with the modified cutoff, as a screening assay for the detection of platelet alloantibodies.
Boehlen
F
F
Medical Clinics II, Department of Medicine, University Hospital, Geneva, Switzerland.
Bulla
O
O
de Moerloose
P
P
D000911:Antibodies, Monoclonal; D000941:Antigens; D001792:Blood Platelets / Q000276:immunology*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007518:Isoantibodies / Q000097:blood*; D008431:Maternal-Fetal Exchange / Q000276:immunology; D011247:Pregnancy; D012372:ROC Curve; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013921:Thrombocytopenia / Q000175:diagnosis / Q000209:etiology / Q000276:immunology
D000911:Antibodies, Monoclonal; D000941:Antigens; D007518:Isoantibodies