[Invasion of Escherichia coli A2 induces reorganization of actin microfilaments in Hep-2 cells].
Bacteria of spontaneously isolated non-pathogenic strain E. coli A2 have been previously shown to produce a new proteinase, referred to as protease ECP 32, which specifically cleaves actin (Khaitlina et al., 1988; Matveyev et al., 1996). Similar proteinase activity was found in revertants of Shigella flexneri L-forms. In this work immunofluorescence and electron microscopy were used to address a question of whether E. coli A2 can invade epithelial cells similarly as it has been demonstrated for Sh. flexneri. Infection of Hep-2 cells with E. coli A2 resulted in bacterial invasion of the cells followed by cytoskeleton reorganization. On one end of intracellular bacteria bundles of actin filaments resembling a comet-like tail were observed. Bacteria of referent strain CCM 5172, not producing protease ECP 32, were not taken up by the cells. These data suggest that protease ECP 32 may be involved in the process of bacterial invasion and cytoskeleton reorganization.
Efremova
T N
TN
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Ender
N A
NA
Brudnaia
M S
MS
Komissarchik
Ia Iu
IaIu
Khaĭtlina
S Iu
SIu
D000199:Actins / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000378:metabolism / Q000648:ultrastructure*; D004847:Epithelial Cells / Q000378:metabolism* / Q000648:ultrastructure*; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000473:pathology*; D006801:Humans; D014407:Tumor Cells, Cultured
D000199:Actins
Reorganizatsiia aktinovykh mikrofilamentov v kletkakh HEP-2 v rezul' tate invazii bakteriĭ Escherichia coli A2.