Short- and intermediate-term carcinogenicity testing--a review. Part 1: the prototypes mouse skin tumour assay and rat liver focus assay.
Carcinogenicity testing is by far the most expensive and time-consuming study type of toxicology. For many years, the lifetime exposure with the maximum tolerated dose in two rodent species has been the gold standard of carcinogenicity testing of pharmaceuticals. Major change was introduced by the Fourth International Conference on Harmonization in July 1997; a chronic rodent bioassay in one species and a short-term carcinogenicity assay are regarded as sufficient for registration. Such requirements provide the opportunity to redirect the vast resources previously spent on the lifetime study in the second species. Numerous experimental protocols for short- and intermediate-term carcinogenicity testing in many target tissues have been available for years. The first part of this review describes the basic principles of short- and intermediate-term carcinogenicity testing using the examples of the widely used mouse skin tumour assay and the rat liver foci assay. In the context of these experimental models, the discrimination and quantification of initiating and promoting activity and the use of preneoplastic lesions as endpoints in carcinogenicity testing are described. The review includes the limitations of the models with regard to the extrapolation from effects observed in animal experiments to a potential exposure of humans.
Enzmann
H
H
Bayer AG, Institute of Toxicology, Wuppertal, Germany.
Bomhard
E
E
Iatropoulos
M
M
Ahr
H J
HJ
Schlueter
G
G
Williams
G M
GM
D000367:Age Factors; D000818:Animals; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000009:adverse effects / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008099:Liver / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced*; D051379:Mice; D011230:Precancerous Conditions / Q000139:chemically induced*; D051381:Rats; D012878:Skin Neoplasms / Q000139:chemically induced*; D013997:Time Factors
D002273:Carcinogens