[In vitro expansion of human umbilical cord blood hematopiotic cells and its subpopulation].
OBJECTIVE
To study the effect of expansion of cord blood stem cells in vitro.


METHODS
The mononuclear cells of human umbilical cord blood was cultured in vitro and the cell cycle and CD34+ subset analysis was performed on these cell preparations using flow cytometry.


RESULTS
After 35 days, the number of nucleated cells showed over a comulative 252 fold expansion. CD34+ cells increased 50-fold in 7-21 days. CD33+ cells increased 280-fold during 7-28 days. CD19+ and CD34+ cells disappared after 14 days. The cells remained in G0/G1 phase and increased in the S phase.


CONCLUSIONS
Single umbilical cord blood would be enough to transplant to adults if suitable time and a novel expansion strategies are set.
Hematology Department, Second Hospital, Suzhou Medical College.
X S
XS
Cao
Z
Z
Zou
W
W
Yu
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis*; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D002453:Cell Cycle; D002455:Cell Division; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D063268:Sialic Acid Binding Ig-like Lectin 3
D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; C571163:CD33 protein, human; D063268:Sialic Acid Binding Ig-like Lectin 3