Stat3 and NFkappaB glial expression after excitotoxic damage to the postnatal brain.
The nuclear factor-kappa B (NFkappaB) and the signal transducer activator of transcription 3 (STAT3), are putative transcription factors activated by growth factors and cytokines, and involved in glial gene expression changes after neuronal injury. Immunocytochemical analysis of NFkappaB and STAT3 from 2 h to 14 days after excitotoxic damage to the postnatal rat brain showed STAT3- and NFkappaB-positive glial cells at 2 h post-lesion, increasing in number to reach a maximum at day 1. Immunoreactivity then decreased but the glial scar remained positive. Glial STAT3 immunoreactivity was located in the nucleus up to 1 day post-lesion and in the nucleus, cytoplasm and cell processes from day 3. Glial NFkappaB immunoreactivity was mainly cytoplasmatic.
Faculty of Medicine, Department of Cell Biology and Physiology, Autonomous University of Barcelona, Bellaterra, Spain.
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L
Acarin
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González
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B
Castellano
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D000818:Animals; D000831:Animals, Newborn; D001927:Brain Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D018846:Excitatory Amino Acids / Q000633:toxicity*; D007150:Immunohistochemistry; D016328:NF-kappa B / Q000096:biosynthesis*; D051381:Rats; D050796:STAT3 Transcription Factor; D015534:Trans-Activators / Q000096:biosynthesis*
D000209:Acute-Phase Proteins; D004268:DNA-Binding Proteins; D018846:Excitatory Amino Acids; D016328:NF-kappa B; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D015534:Trans-Activators