Collagen type XVI expression is modulated by basic fibroblast growth factor and transforming growth factor-beta.
We investigated the effects of bFGF and TGF-beta on the expression of type XVI collagen, a member of the fibril associated collagen family, in human dermal fibroblasts and arterial smooth muscle cells. We found that bFGF decreased the alpha1(XVI) collagen mRNA to 18-24% of the controls, while TGF-beta increased the mRNA to 150-360%. Immunoprecipitation of metabolically labeled cells revealed corresponding, but less pronounced, changes at the protein levels. The results suggested that type XVI collagen expression is regulated by bFGF and TGF-beta in a manner similar to their regulation of the major type I fibrillar collagen produced by these cells.
Grässel
S
S
Dept. of Dermatology and Cutaneous Biology, Thomas Jefferson University, Philadelphia, PA 19107, USA.
Tan
E M
EM
Timpl
R
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Chu
M L
ML
D001158:Arteries / Q000378:metabolism; D001940:Breast; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007700:Kinetics; D009131:Muscle, Smooth, Vascular / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012867:Skin / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D003094:Collagen