Morphogenesis, biomass and oxytetracycline production of Streptomyces rimosus in submerged cultivation.
The minimal concentration of adenosine triphosphate (ATP) which could be detected with spectrophotometry, HPLC and luciferin-luciferase methods was 1.0 microM, 3.3 microM and 100 nM, respectively. In submerged cultivation, most Streptomyces rimosus TM-55 was in hyphae fragment form at 65 h, became short-rod mycelia at 166 h, and lysed at 504 h incubation. The ATP content had maximal value at 24 h, then gradually decreased during cultivation. The oxytetracycline potency increased as incubation occurred, had maximal potency 178.9 micrograms/ml at 166 h, and then gradually decreased. Morphogenesis was very important in oxytetracycline production in submerged cultivation of Streptomyces; short-rod mycelia had high oxytetracycline production.
Wang
J Y
JY
Department of Agricultural Chemistry, National Taiwan University, Taipei, Republic of China.
Yang
S S
SS
D000227:Adenine Nucleotides / Q000032:analysis; D018533:Biomass; D009024:Morphogenesis; D010118:Oxytetracycline / Q000096:biosynthesis*; D013302:Streptomyces / Q000254:growth & development / Q000378:metabolism*
D000227:Adenine Nucleotides; D010118:Oxytetracycline