Capillary electrophoresis interfaced to inductively coupled plasma mass spectrometry for element selective detection in arsenic speciation.
A method is presented to separate and detect six arsenic species by capillary electrophoresis (CE) interfaced to inductively coupled plasma mass spectrometry (ICP-MS). CE was used as a highly resolving separation system, whereas ICP-MS served as an element selective detector providing low detection limits. The special mode of operation included sample stacking and a differentiation of separation and detection. This provided separation and detection of six As species, uncharged and anionic, to be monitored within a single run. Detection limits were calculated according to IUPAC recommendation at 15 microg As/L for As (III), dimethyl arsinic acid (DA), monomethyl arsonic acid (MA) and As (V), or 65 microg As/L for arsenobetaine (AsB) and arsenocholine (AsC). Investigations were focused on possibly occurring interferences, e.g., ArCl+ interference at the monoisotope 75As. Finally, real samples from biomedical field (urine) and environmental field (sewage sludge) were analyzed.
GSF-National Research Center for Environment and Health, Institute for Ecological Chemistry, Neuherberg, Germany. bernhard.michalke@gsf.de
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Schramel
D000838:Anions; D001151:Arsenic / Q000652:urine; D001152:Arsenicals / Q000032:analysis* / Q000302:isolation & purification; D002101:Cacodylic Acid / Q000032:analysis; D002712:Chlorides / Q000652:urine; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008832:Microchemistry; D012722:Sewage
D000838:Anions; D001152:Arsenicals; D002712:Chlorides; D012722:Sewage; C039920:arsenocholine; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; D001151:Arsenic; C038992:arsenobetaine