A strategy for the generation of infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome.
A strategy to generate in vitro transcripts representing infectious RNAs and autonomously replicating RNAs based on the HCV 229E genome is presented. PCR-DNAs were ligated in vitro, resulting in 27 kbp and 22 kbp ligation products. These DNAs can now be transcribed in vitro and the RNAs tested for infectivity and their ability to replicate.
Herold
J
J
Institute for Virology and Immunology, University of Würzburg, Germany.
Thiel
V
V
Siddell
S G
SG
D017934:Coronavirus / Q000235:genetics* / Q000472:pathogenicity; D028941:Coronavirus 229E, Human; D004279:DNA, Viral; D016679:Genome, Viral; D006801:Humans; D012367:RNA, Viral / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral