Cytogenetic clonality analysis in monosomy 7 associated with juvenile myelomonocytic leukemia: clonality in B and NK cells, but not in T cells.
It remains unclear which lymphoid lineages are involved in juvenile myelomonocytic leukemia (JMML). We report a JMML patient who acquired monosomy 7 after intensive chemotherapy. In this case, the expression of monosomy 7 was analyzed in T, B and natural killer (NK) cells highly purified from peripheral blood mononuclear cells of the patient. The fluorescence in situ hybridization method revealed the expression of monosomy 7 in B cells, but not T cells. Half of the NK cells expressed monosomy 7; when NK cells were divided into CD2- and CD2+ populations, this abnormality was positive in 91.1% of CD2- NK cells but in only 14.7% of CD2+ NK cells. These results suggest that, in this JMML patient who acquired monosomy 7 after intensive chemotherapy, B cells and half of NK cells, but not T cells, have monosomy 7.
Department of Pediatrics, Shinshu University School of Medicine, Asahi, Matsumoto, Japan.
T
T
Nakazawa
K
K
Koike
K
K
Agematsu
S
S
Itoh
R
R
Hagimoto
Y
Y
Kitazawa
T
T
Higuchi
N
N
Sawai
H
H
Matsui
A
A
Komiyama
D001402:B-Lymphocytes; D018801:CD2 Antigens / Q000097:blood; D019070:Cell Lineage; D002897:Chromosomes, Human, Pair 7; D002999:Clone Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D007621:Karyotyping; D007694:Killer Cells, Natural; D015477:Leukemia, Myelomonocytic, Chronic / Q000235:genetics*; D008297:Male; D009006:Monosomy; D013601:T-Lymphocytes
D018801:CD2 Antigens