[Effect of refined xuefu capsule on mRNA expression of nitric oxide synthase of hypoxic ischemic myocytes evaluated by northern blotting].
Comparative study on effect of refined Xuefu capsule, Xuefu Zhuyu capsule and diltiazem on mRNA expression of nitric oxide synthase (NOS) and release of LDH-L, CK, GOT of hypoxic hypoglycemic cultured myocytes were studied by using the serum pharmacological method, reverse transcriptase-polymerase chain reaction (RT-PCR) and Northen blotting. It was shown that the three drugs can reduce release of LDH-L,CK,GOT of myocytes (P < 0.01), the effect of refined Xuefu capsule and diltiazem were obvious than that of Xuefu Zhuyu capsule in reducing LDH-L release and NOS mRNA expression (P < 0.01), and effect of refined Xuefu capsule was the strongest (P < 0.01). These findings indicated that all 3 drugs have significant effect in protecting myocytes which related to their effect on NO production of myocyte particularly the refined Xuefu capsule and diltiazem.
Wang
W
W
Xiyuan Hospital, China Academy of TCM, Beijing.
Chen
K J
KJ
Shi
D Z
DZ
D000818:Animals; D015687:Cell Hypoxia; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D051381:Rats
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D019001:Nitric Oxide Synthase