[Measuring cyclosporine levels in whole blood in kidney transplantation].
BACKGROUND
Cyclosporin A is a potent immunosuppressive drug effective in combatting rejection following organ transplantation. In na effort to replace a radioimmunoassay (RIA) for whole blood determination of cyclosporine (Cya) we compared RIA with fluorescence polarization immunoassay (FPIAm).


METHODS
65 blood samples were analysed from kidney transplanted patients. The samples were collected into tubes containing EDTA as anticoagulant and analysed by RIA and FPIAm.


RESULTS
The statistical analysis revealed a difference between both methods (p < 0.05). The linear-regression comparison of Cya concentration measured by RIA and FPIAm showed the following relationship: Cya(FPIAm) = 1.06 x Cya(RIA) + 5.8 (r = 0.9817).


CONCLUSIONS
We conclude that FPIAm provides na alternative method for measuring cyclosporine in whole blood with the added advantages of being reasonably rapid, precise and easy to perform.
Departamento de Patologia Clínica, Faculdade de Ciências Médicas, UNICAMP, Campinas, SP.
P V
PV
Bottini
F A
FA
Alves-Cunha
M A
MA
Moda
M I
MI
Souza
C R
CR
Garlipp
D016572:Cyclosporine / Q000097:blood* / Q000627:therapeutic use; D005260:Female; D016231:Fluorescence Polarization Immunoassay; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood* / Q000627:therapeutic use; D016030:Kidney Transplantation; D008297:Male; D011863:Radioimmunoassay; D012044:Regression Analysis; D015203:Reproducibility of Results
D007166:Immunosuppressive Agents; D016572:Cyclosporine
Monitoramento dos níveis de ciclosporina em sangue total em transplantes renais.