PCR-based identification of clinically relevant Pseudallescheria/Scedosporium strains.
Pseudallescheria boydii (anamorph: Scedosporium apiospermum) and S. prolificans can cause severe infections in both the immunocompromized host and accidentally injured people. A species-specific polymerase chain reaction (PCR) enabling detection and discrimination of these fungi has not been available to date. In view of the difficult treatment especially of S. prolificans infections, a PCR-based detection system has been developed. Based on results of partial sequencing of ribosomal DNA, Scedosporium DNA could be amplified, either at the genus or at the species level. Hybridization probes for the identification of the PCR-products were established.
Berlin University of Technology, Germany, FB 15, Department of Microbiology and Genetics, Berlin, Germany.
M
M
Wedde
D
D
Müller
K
K
Tintelnot
G S
GS
De Hoog
U
U
Stahl
D001483:Base Sequence; D015139:Blotting, Southern; D004275:DNA, Ribosomal / Q000235:genetics; D006801:Humans; D016867:Immunocompromised Host; D008969:Molecular Sequence Data; D008271:Mycetoma / Q000382:microbiology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011541:Pseudallescheria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage; D012988:Soil Microbiology; D014947:Wounds and Injuries / Q000382:microbiology
D004275:DNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; D012722:Sewage