Chromatographic separation and automated analysis of flavanols.
Flavanols from barley or hops were separated chromatographically and assayed automatically by reaction with the chromogen, 4-dimethylaminocinnamaldehyde. For separating the flavanols on Sephadex G-25, gradient elution with water-methanol mixtures was necessary. The chromogen was specific for flavanols and well suited to AutoAnalyzer methods. The method appears generally applicable in flavanol analysis of plant materials.
Research Laboratory, A. Guinness Son & Co. (Dublin) Ltd., St. James's Gate, Ireland.
I
I
McMurrough
J
J
McDowell
D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002934:Cinnamates; D003911:Dextrans; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D001467:Hordeum / Q000737:chemistry; D007202:Indicators and Reagents; D000432:Methanol; D014867:Water
D002934:Cinnamates; D003911:Dextrans; D005419:Flavonoids; D007202:Indicators and Reagents; D014867:Water; C025614:sephadex; C018523:4-dimethylaminocinnamaldehyde; D000432:Methanol