Acetylcholine release. Reconstitution of the elementary quantal mechanism.
Choline acetyltransferase and vesicular acetylcholine transporter genes are the products of two adjacent genes defining a cholinergic locus. The release mechanism is expressed independently of this locus in some cell lines. A cholinergic neuron will therefore have to coordinate the expression of release with that of the cholinergic locus. Transfection of a plasmid encoding Torpedo mediatophore in cells that are unable to release this transmitter endows them with a Ca2(+)-dependent and quantal release mechanism. The synchronization of mediatophore activation results from a control of calcium microdomains by the synaptic vesicles. It is therefore dependent on the proteins that dock vesicles close to calcium channels.
Israël
M
M
Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, Gif-sur-Yvette, France.
Dunant
Y
Y
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002795:Choline O-Acetyltransferase / Q000235:genetics / Q000502:physiology*; D006801:Humans; D026901:Membrane Transport Proteins; D009474:Neurons / Q000378:metabolism*; D011789:Quantum Theory; D011994:Recombinant Proteins / Q000378:metabolism; D014101:Torpedo; D014162:Transfection; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins
D002352:Carrier Proteins; D026901:Membrane Transport Proteins; D011994:Recombinant Proteins; C493459:SLC18A3 protein, human; D050494:Vesicular Acetylcholine Transport Proteins; D033921:Vesicular Transport Proteins; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine