A new method to characterize saturation functions by their first four moments.
The calculation of the first four moments of saturation functions is proposed as a method to describe the properties of enzymes or receptors models. The values of these moments in the case of the Langmuir or Michaelis-Menten equation and the Hill equation are reviewed. They have been calculated for the second degree Adair equation and in the case of binding site heterogeneity. A method for generalization to cases of greater complexity is also proposed. The advantage of this method over the classical ones--graphical representations and derivation of coefficients like nH, [L]0.9/[L]0.1 ...--is essentially that the moments are defined by one single value independently of any particular model for the whole of the saturation curve.
Boeynaems
J M
JM
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
Cantraine
F R
FR
D001665:Binding Sites; D001699:Biometry; D004798:Enzymes / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D016015:Logistic Models; D008954:Models, Biological; D011485:Protein Binding
D004798:Enzymes; D008024:Ligands