Structure of type III antifreeze protein at 277 K.
Fish antifreeze proteins (AFP's) depress the freezing point of blood and other body fluids below that of the surrounding seawater by binding to and inhibiting the growth of seed ice crystals. The high-resolution crystal structure of type III AFP, determined at room temperature, reveals a remarkably flat surface containing most of the ice-binding residues [Jia et al. (1996). Nature (London), 384, 285-288]. Since AFP's function at temperatures close to 273 K, it is important to know whether the structure determined at room temperature undergoes any change at much lower temperature. Therefore, type III AFP has been crystallized at 277 K and its structure determined. Although crystallization conditions at 277 K were similar to those at approximately 295 K, crystal growth took much longer at the lower temperature. Crystals grown at the two temperatures were isomorphous. Initial crystals appeared within 40-50 d and grew to their final size in about 8-12 months, instead of a couple of days at approximately 295 K. The type III antifreeze protein structure from crystals grown at 277 K was essentially the same as that determined at approximately 295 K, with the exception of some minor changes in side-chain conformation. The result is an indication that temperature has a minimal effect on the structure of type III AFP, thus lending increased physiological validity to the room-temperature structure which was used for the initial ice-binding modelling.
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Department of Biochemistry, Queen's University, Kingston, Ontario, K7L 3N6 Canada.
Leinala
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Jia
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D000818:Animals; D021301:Antifreeze Proteins; D003080:Cold Temperature; D003460:Crystallization; D018360:Crystallography, X-Ray; D005399:Fishes / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry* / Q000302:isolation & purification; D008958:Models, Molecular; D011487:Protein Conformation; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000302:isolation & purification
D021301:Antifreeze Proteins; D006023:Glycoproteins; D011993:Recombinant Fusion Proteins