Immunohistochemical demonstration of carcinogen-DNA adducts in tissues of rats given 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP): detection in paraffin-embedded sections and tissue distribution.
A polyclonal antibody against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-DNA adducts was raised for their immunohistochemical demonstration in paraffin-embedded sections. Specificity of this antibody was confirmed by competitive ELISA. Positive signals were immunohistochemically detected in acetone-fixed but not in formalin- or ethanol-fixed sections from F344 rats treated by gavage with a single dose of PhIP at 37.5-300 mg/kg and killed at 1, 2, and 7 days thereafter. Dose-dependent positive staining was observed in almost all organs of both sexes, including the colon, prostate, and mammary gland but largely independent of the tumor response. Repair activity, judged by disappearance of adducts with time, differed according to the organ or cell type. One exception was hepatocytes, the liver incidentally being a nontarget organ. The results suggest that the generated antibody is applicable for detection of cells targeted by PhIP in paraffin-embedded sections and also for the investigation of the mechanisms of PhIP-carcinogenesis.
First Department of Pathology, Nagoya City University Medical School, Nagoya, Japan. sattak@med.nagoya-cu.ac.jp
S
S
Takahashi
S
S
Tamano
M
M
Hirose
N
N
Kimoto
Y
Y
Ikeda
M
M
Sakakibara
M
M
Tada
F F
FF
Kadlubar
N
N
Ito
T
T
Shirai
D000284:Administration, Oral; D000818:Animals; D002273:Carcinogens / Q000008:administration & dosage / Q000493:pharmacokinetics*; D003106:Colon / Q000166:cytology / Q000378:metabolism; D003429:Cross Reactions; D018736:DNA Adducts / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007093:Imidazoles / Q000008:administration & dosage / Q000493:pharmacokinetics*; D007150:Immunohistochemistry; D008297:Male; D008321:Mammary Glands, Animal / Q000166:cytology / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000139:chemically induced* / Q000473:pathology; D011467:Prostate / Q000166:cytology / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D014018:Tissue Distribution
D002273:Carcinogens; D018736:DNA Adducts; D007093:Imidazoles; C049584:2-amino-1-methyl-6-phenylimidazo(4,5-b)pyridine