[An additional dehydrogenase or oxidation-reduction test in the classification of enterobacteria].
The dehydrogenase (DHG) or oxidation-reduction test is proposed for use together with the determination of such enzymes as hydrolases, cytochrome oxidase, dehydrocarboxylase, urease, etc. 200 Citrobacter freundii cultures and 76 strains of enteropathogenic Escherichia (EPE) were studied with the determination of their DHG activity in semiliquid mannitol and in Kligler's medium. The study revealed that this test, characterized by the reduction of the indicator, similarly to that in salmonellae and shigellae, was constantly negative in semiliquid mannitol in C. freundii and in 97.3% of cases in EPE. In 17.5% of C. freundii lactose-positive cultures the DHG test in Kligler's medium was positive, which made it possible to regard them as a separate biovar. Taking into account the results of this investigation, the subdivision of C. freundii into 3 biovars is proposed.
Bril'man
Ia E
IaE
City Infectious Hospital, Kaliningrad, Russia.
D001431:Bacteriological Techniques; D016971:Citrobacter freundii / Q000145:classification* / Q000201:enzymology; D003470:Culture Media; D004925:Escherichia / Q000145:classification* / Q000201:enzymology; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D007202:Indicators and Reagents; D010088:Oxidoreductases
Dopolnitel'nyĭ degidrogenaznyĭ ili okislitel'no-vosstanovitel'nyĭ test v klassifikatsii énterobakteriĭ.