Immunogenicity of purified lipopolysaccharide or protein-oligosaccharide conjugates of Pasteurella multocida type 6:B in mice.
Purified lipopolysaccharide (LPS) of Pasteurella multocida type 6:B, while toxic at higher doses, was protective at lower dose levels against experimentally-induced pasteurellosis in mice. However, the observed protection was abrogated if such LPS was digested with proteinase K prior to use in immunisation. The O-antigen polysaccharide side-chain (OS) of LPS did not appear to contribute to the observed protection as judged by the fact that immunisation of mice with purified OS or OS-protein conjugates, all of which were nontoxic, failed to confer protection against challenge with homologous virulent organisms. This was despite generation of significant levels of OS-specific antibodies, predominantly either of the IgM or IgG isotypes, in immunised mice.
Veterinary Research Institute, Ipoh, Malaysia.
N
N
Muniandy
D N
DN
Love
T K
TK
Mukkur
D000272:Adipates; D000586:Amination; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006433:Hemocyanins; D015151:Immunoblotting; D008070:Lipopolysaccharides / Q000276:immunology* / Q000633:toxicity; D051379:Mice; D008807:Mice, Inbred BALB C; D019081:O Antigens / Q000276:immunology / Q000302:isolation & purification; D009844:Oligosaccharides / Q000276:immunology; D010326:Pasteurella Infections / Q000276:immunology / Q000517:prevention & control; D016979:Pasteurella multocida / Q000276:immunology*; D011506:Proteins / Q000276:immunology*; D012710:Serum Albumin, Bovine
D000272:Adipates; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D019081:O Antigens; D009844:Oligosaccharides; D011506:Proteins; D012710:Serum Albumin, Bovine; C029900:adipic acid; D006433:Hemocyanins