Effects of Arg-Gly-Asp-Ser on Ca2+ transport of myocardial sarcoplasmic reticulum in rat septic shock.
OBJECTIVE
To study the effects of Arg-Gly-Asp-Ser (RGDS), a synthetic short peptide of fibrinogen degradation, on the Ca2+ transport function of cardiac sarcoplasmic reticulum in rat septic shock.


METHODS
RGDS 5 mumol.kg-1 was injected i.v. at 4 h and 14 h after cecal ligation and puncture (CLP) operation on rats. Highly purified membrane of sarcoplasmic reticulum (SR) was prepared from rat hearts. Assays were made of ATP-dependent Ca2+ uptake by cardiac SR and [3H] ryanodine binding to SR.


RESULTS
The initial rate and the capacity of SR Ca2+ uptake were increased by 104% (P < 0.01) and 12% (P < 0.05), respectively, paralleled by an increase in Ca(2+)-ATPase activity and a decrease in calcium accumulation of myo- cardium of septic rats, whereas the Bmax and Kd values of Ca2+ activated [3H]ryanodine binding to SR were unaffected after RGDS administration.


CONCLUSIONS
The results indicated that RGDS have cardioprotective effects of maintaining Ca2+ homeostasis of cardiac myocytes by enhancing SR Ca2+ uptake in rat septic shock.
Department of Pathophysiology, School of Basic Medical Sciences, Beijing, China.
Y
Y
Ji
M
M
Zhao
Y
Y
Qi
L W
LW
Dong
L L
LL
Wu
S Q
SQ
Peng
J Y
JY
Su
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D005340:Fibrinogen / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D009842:Oligopeptides / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*; D012772:Shock, Septic / Q000378:metabolism*
D009842:Oligopeptides; D010975:Platelet Aggregation Inhibitors; D005340:Fibrinogen; C046617:arginyl-glycyl-aspartyl-serine; D002118:Calcium