Cloning and genetic mapping of zebrafish BMP-2.
The BMP family of polypeptide growth factors has been shown to play diverse roles in establishing embryonic patterning and tissue fates. We report the cloning of the zebrafish homologue of BMP-2, examine its expression during embryogenesis, and find that it is localized to the distal end of the long arm of zebrafish chromosome 20. A missense mutation of the bmp2 gene has recently been shown to be responsible for the early dorsalized phenotype of the zebrafish swirl mutant [Kishimoto et al., 1997]. Given the dynamic expression of bmp2 in the developing embryo and the complex interactions of BMP signaling response in vertebrates, it is possible that other mutant phenotypes, due to altered bmp2 gene expression, will eventually map to or interact with this genetic locus.
Department of Cardiology, Children's Hospital, Boston, Massachusetts, USA.
K H
KH
Lee
J J
JJ
Marden
M S
MS
Thompson
H
H
MacLennan
Y
Y
Kishimoto
S J
SJ
Pratt
S
S
Schulte-Merker
M
M
Hammerschmidt
S L
SL
Johnson
J H
JH
Postlethwaite
D C
DC
Beier
L I
LI
Zon
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis / Q000235:genetics*; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004625:Embryo, Nonmammalian / Q000378:metabolism / Q000648:ultrastructure; D018507:Gene Expression Regulation, Developmental; D005796:Genes; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D018076:DNA, Complementary; D016212:Transforming Growth Factor beta