[The amounts of inositol 1,4,5-triphosphate and it response to epidermal growth factor and laminin of carcinoma substrains with high or low metastatic potentials].
OBJECTIVE
To examine the IP3 amounts, invasive potentials, and response to LN and EGF of the substrains with high or low metastatic ability from two kinds of carcinoma cell lines (MFC and CNZ-2Z).


METHODS
Radio-ligand binding assay and matrigel invasive assay.


RESULTS
The low metastatic substrains from the two lines had higher amounts of IP3 and stronger response to EGF than their responsive high ones, but high metastatic substrains from CNZ-2Z had stronger response to LN than the low ones while MFC substrains had not significant difference in response to LN.


CONCLUSIONS
There is internal difference in signal transduction between the high and low metastatic cancer cells, and it is significant to study the difference in detail.
Department of Pathology, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing.
X
X
Hu
J
J
Gao
Y
Y
Li
D000818:Animals; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006801:Humans; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007797:Laminin / Q000378:metabolism*; D051379:Mice; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D015398:Signal Transduction; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D014407:Tumor Cells, Cultured
D007797:Laminin; D004815:Epidermal Growth Factor; D015544:Inositol 1,4,5-Trisphosphate