The analgesic effects of tryptophan and its metabolites in the rat.
Male Sprague-Dawley rats weighing 150-200 g were given doses of tryptophan methyl ester or its metabolites; kynurenine sulphate, kynurenic acid, xanthurenic acid, quinolinic acid, anthranilic acid methyl ester or picolinic acid methyl ester. Doses administered intraperitoneally were 50, 100, 200, 300, 400 and 600 mg kg-1. Pain sensitivity was assessed using the hotplate and tailflick methods at 30 min before and at 30-min interval after the injection of test compounds. The administrations of tryptophan, kynurenic acid, quinolinic acid, anthranilic acid, xanthurenic acid, picolinic acid, and kynurenine were associated with analgesia. Animals given 300 or 600 mg kg-1 of tryptophan exhibited a significant decrease (P<0.05; P<0.01, respectively) in pain sensitivity with the hotplate test. l-Kynurenic acid (300 mg kg-1) produced analgesia (P<0.01) 30 min after drug administration. Quinolinic and anthranilic acids both produced prolonged decrease in pain sensitivity (P<0.05) using the tailflick test. These results indicate that tryptophan and some of its metabolites possess analgesic properties.
College of Pharmacy and Pharmaceutical Sciences, Florida A & M University, Tallahassee, FL 32307, USA.
S O
SO
Heyliger
C B
CB
Goodman
J M
JM
Ngong
K F
KF
Soliman
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000494:pharmacology; D007502:Iron Chelating Agents / Q000494:pharmacology; D007736:Kynurenic Acid / Q000494:pharmacology; D007737:Kynurenine / Q000494:pharmacology; D008297:Male; D010146:Pain / Q000503:physiopathology; D010848:Picolinic Acids / Q000494:pharmacology; D017378:Quinolinic Acid / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000187:drug effects; D012018:Reflex / Q000187:drug effects; D014364:Tryptophan / Q000378:metabolism* / Q000494:pharmacology*; D014977:Xanthurenates / Q000494:pharmacology; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D000700:Analgesics; D000927:Anticonvulsants; D018691:Excitatory Amino Acid Antagonists; D000960:Hypolipidemic Agents; D007502:Iron Chelating Agents; D010848:Picolinic Acids; D014977:Xanthurenates; D062367:ortho-Aminobenzoates; C031385:anthranilic acid; D007737:Kynurenine; C028330:xanthurenic acid; D014364:Tryptophan; D017378:Quinolinic Acid; D007736:Kynurenic Acid; C030614:picolinic acid