Comparison of four serological tests to determine the CagA or VacA status of Helicobacter pylori strains.
We compared four tests for antibodies to CagA or VacA, HelicoBlot 2. 0, RIDA Blot Helicobacter, CHIRON RIBA H. pylori SIA, and an enzyme-linked immunosorbent assay using recombinant CagA. Immunoblot assays were accurate for determining Helicobacter pylori status but poor for determining CagA or VacA status (accuracy, 66 to 80% for CagA status and 34 to 67% for VacA status). None can be recommended for determining CagA or VacA status.
Yamaoka
Y
Y
Department of Medicine, Veterans Affairs Medical Center and Baylor College of Medicine, Houston, Texas 77030, USA. yoshio@wt.net
Kodama
T
T
Graham
D Y
DY
Kashima
K
K
D000942:Antigens, Bacterial / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology; D001431:Bacteriological Techniques; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data; D005069:Evaluation Studies as Topic; D016481:Helicobacter Infections / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D006801:Humans; D015151:Immunoblotting / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori