Amidolytic and peptidolytic activities of immunoglobulin G present in sera from patients with rheumatoid arthritis, Sjogren's syndrome and systemic lupus erythematosus.
Polyclonal immunoglobulin G (IgG) from healthy subjects was found to be capable of hydrolyzing carbobenzoxy-Val-Gly-Arg-p-nitroanilide (a synthetic chromogenic substrate for trypsin) and D-Pro-Phe-Arg-p-nitroanilide (a substrate for plasma kallikrein). Statistically significant elevation of activity against the former substrate was found in patients with rheumatoid arthritis (RA), but not in patients with Sjogren's syndrome (SjS) or systemic lupus erythematosus (SLE). On the other hand, IgG samples from the patients with these three autoimmune diseases showed reduced activity against d-Pro-Phe-Arg methylcoumarinamide, although the differences were not statistically significant. Preliminary studies have shown that two out of three IgG samples from RA patients exhibited the activity of cleaving a pentapeptide, Gln-Arg-Arg-Ala-Ala, whereas virtually no cleavage of the same peptide was observed with IgG from healthy controls or from patients with SjS or SLE.
Third Department of Internal Medicine, Kinki University School of Medicine, Osaka, Japan.
K
K
Matsuura
S
S
Ikoma
M
M
Sugiyama
M
M
Funauchi
H
H
Sinohara
D000328:Adult; D000577:Amides / Q000378:metabolism*; D000814:Aniline Compounds / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D002863:Chromogenic Compounds / Q000378:metabolism; D000939:Epitopes / Q000378:metabolism; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D006868:Hydrolysis; D007074:Immunoglobulin G / Q000378:metabolism*; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009842:Oligopeptides / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism*; D012859:Sjogren's Syndrome / Q000276:immunology; D018709:Statistics, Nonparametric
D000577:Amides; D000814:Aniline Compounds; D002863:Chromogenic Compounds; D000939:Epitopes; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D009842:Oligopeptides; D010446:Peptide Fragments; C019498:4-nitroaniline; C026148:prolyl-phenylalanyl-arginine-4-nitroanilide