Elevated expression of chitinase 1 and chitin synthesis in myosin II-deficient Saccharomyces cerevisiae.
To determine if the attached cells formed in Myosin II-deficient Saccharomyces cerevisiae result from deficient chitinase 1 (CTS1) expression, the activity of chitinase 1 was assayed. Secretion of this enzyme was not prevented by a MYO1 gene deficiency, and soluble and cell wall-associated Cts1p activity were increased approximately 5-fold and 20-fold, respectively, in these cells. The increase in soluble activity was correlated with an increase in enzyme levels. Likewise, intracellular chitinase activity was increased approximately 22-fold, and the chitin content of cell walls was elevated 2-fold. These data suggest that the origin of myo1-associated phenotypes is not due to deficient chitinase expression and may instead be due to a deregulation of cell wall metabolism in these cells.
Rodríguez-Medina
J R
JR
Department of Biochemistry, University of Puerto Rico, Medical Sciences Campus, San Juan 00936-5067, USA.
Cruz
J A
JA
Robbins
P W
PW
Bi
E
E
Pringle
J R
JR
D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism; D002686:Chitin / Q000096:biosynthesis*; D002688:Chitinases / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000172:deficiency*; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins
D005656:Fungal Proteins; C112936:MYO1 protein, S cerevisiae; D009097:Multienzyme Complexes; D029701:Saccharomyces cerevisiae Proteins; C025310:lyticase; D002686:Chitin; D002688:Chitinases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases; D018995:Myosin Heavy Chains