Effect of cytokinin 4PU30 on organ differentiation and dihydroisocoumarin production in amacha callus cultures.
Effect of a suitable combination of 1-naphthaleneacetic acid (NAA) and cytokinin N-(2-chloro-4-pyridyl)-N'-phenylurea (4PU30) concentrations, regarding organ differentiation and dihydroisocoumarin production, was examined in Hydrangea macrophylla var. thunbergii callus cultures. The callus cultured in a Linsmaier-Skoog medium containing 10(-5) M NAA and 10(-5) M 4PU30 under a light condition gave the highest level for callus growth, shoot formation, and phyllodulcin 8-O-glucoside content.
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
H
H
Yamamoto
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M
Yamamoto
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K
Yamamoto
N
N
Chatani
M
M
Yoshikawa
D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D003374:Coumarins / Q000378:metabolism*; D007701:Kinetin; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010671:Phenylurea Compounds / Q000494:pharmacology*; D063245:Plant Development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018521:Plant Physiological Phenomena / Q000187:drug effects*; D010944:Plants / Q000187:drug effects* / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*
D003374:Coumarins; D009280:Naphthaleneacetic Acids; D010671:Phenylurea Compounds; D010937:Plant Growth Regulators; D011725:Pyridines; D015084:2,4-Dichlorophenoxyacetic Acid; C034182:1-naphthaleneacetic acid; D000225:Adenine; C116093:N-(2-chloro-4-pyridyl)-N'-phenylurea; D007701:Kinetin