The preference of tryptophan for membrane interfaces.
One of the ubiquitous features of membrane proteins is the preference of tryptophan and tyrosine residues for membrane surfaces that presumably arises from enhanced stability due to distinct interfacial interactions. The physical basis for this preference is widely believed to arise from amphipathic interactions related to imino group hydrogen bonding and/or dipole interactions. We have examined these and other possibilities for tryptophan's interfacial preference by using 1H magic angle spinning (MAS) chemical shift measurements, two-dimensional (2D) nuclear Overhauser effect spectroscopy (2D-NOESY) 1H MAS NMR, and solid state 2H NMR to study the interactions of four tryptophan analogues with phosphatidylcholine membranes. We find that the analogues reside in the vicinity of the glycerol group where they all cause similar modest changes in acyl chain organization and that hydrocarbon penetration was not increased by reduction of hydrogen bonding or electric dipole interaction ability. These observations rule out simple amphipathic or dipolar interactions as the physical basis for the interfacial preference. More likely, the preference is dominated by tryptophan's flat rigid shape that limits access to the hydrocarbon core and its pi electronic structure and associated quadrupolar moment (aromaticity) that favor residing in the electrostatically complex interface environment.
National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Rockville, Maryland 20852, USA.
W M
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Yau
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Wimley
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Gawrisch
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White
D001425:Bacterial Outer Membrane Proteins; D008051:Lipid Bilayers / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D010713:Phosphatidylcholines / Q000737:chemistry; D018272:Porins; D011991:Receptors, Virus / Q000737:chemistry; D055672:Static Electricity; D013499:Surface Properties; D013816:Thermodynamics; D014364:Tryptophan / Q000031:analogs & derivatives / Q000737:chemistry*; D014867:Water
D001425:Bacterial Outer Membrane Proteins; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D018272:Porins; D011991:Receptors, Virus; C016532:maltoporins; D014867:Water; D014364:Tryptophan; C017251:1,2-oleoylphosphatidylcholine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine