Accelerator mass spectrometry as a bioanalytical tool for nutritional research.
Accelerator Mass Spectrometry is a mass spectrometric method of detecting long-lived radioisotopes without regard to their decay products or half-life. The technique is normally applied to geochronology, but is also available for bioanalytical tracing. AMS detects isotope concentrations to parts per quadrillion, quantifying labeled biochemicals to attomole levels in milligram-sized samples. Its advantages over non-isotopic and stable isotope labeling methods are reviewed and examples of analytical integrity, sensitivity, specificity, and applicability are provided.
Vogel
J S
JS
Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, CA 94551, USA.
Turteltaub
K W
KW
D000818:Animals; D002127:Calcium Isotopes / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis; D002273:Carcinogens / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008954:Models, Biological; D009747:Nutritional Physiological Phenomena; D010315:Particle Accelerators; D011804:Quinolines / Q000652:urine; D011849:Radioactive Tracers; D051381:Rats; D012680:Sensitivity and Specificity
D002127:Calcium Isotopes; D002247:Carbon Isotopes; D002273:Carcinogens; D011804:Quinolines; D011849:Radioactive Tracers; D003903:Deuterium; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline