Molecular characterization of the Drosophila melanogaster gene encoding the pterin 4alpha-carbinolamine dehydratase.
We have isolated and characterized the cDNA and the genomic DNA encoding Drosophila melanogaster pterin 4alpha-carbinolamine dehydratase (PCD). The amino acid sequence deduced from the cDNA sequence was very similar to those of PCDs previously reported in other species (19-57% identity). The protein coding region of the cDNA was expressed in E. coli as a histidine fusion protein, and the expressed protein proved to have PCD activity. The characterization of the Drosophila genomic clone revealed that the Drosophila PCD gene is interrupted by two introns. The potential promoter region, deduced from the determination of the transcription start point (tsp), lacks the distinct TATAAA box consensus sequence.
Seong
C
C
Department and Institute of Genetic Engineering and Natural Sciences, Department of Genetic Engineering, Kyung Hee University, Yongin City 449-701, South Korea.
Jeong
S
S
Park
D
D
Yoon
J
J
Oh
Y
Y
Yim
J
J
Han
K
K
Baek
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017344:Genes, Insect; D006639:Histidine / Q000235:genetics; D006836:Hydro-Lyases / Q000096:biosynthesis / Q000235:genetics*; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D016415:Sequence Alignment
D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; D006639:Histidine; D004247:DNA; D006836:Hydro-Lyases; C076392:pterin-4a-carbinolamine dehydratase