A simplified polyacrylamide gel electrophoresis apparatus for simultaneous application of multiple buffer systems or detergent combinations.
A previous design of an apparatus for the simultaneous fractionation by polyacrylamide gel electrophoresis in 10 different buffer systems (1) was replaced by a greatly simplified new design, employing small, cylindrical buffer partitions within the lower buffer reservoir and/or upper buffer reservoir of a conventional, temperature-regulated polyacrylamide gel electrophoresis apparatus for cylindrical gels. The apparatus was tested in application to the problem of simultaneous polyacrylamide gel electrophoresis in different buffer systems with the purpose of optimizing the operative pH for a particular fractionation problem. It was also applied to fractionations in a single buffer system to which various combinations of ionic and nonionic detergents were admixed.
Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20014, USA.
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Newby
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Matthews
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Chrambach
D000262:Adenylyl Cyclases / Q000302:isolation & purification; D000818:Animals; D002021:Buffers; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation*; D004867:Equipment Design; D019382:Human Growth Hormone / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051381:Rats
D002021:Buffers; D003902:Detergents; D019382:Human Growth Hormone; D000262:Adenylyl Cyclases