Developmental expression of a Fasciola hepatica sequence homologous to ABC transporters.
Polymerase chain reaction and cDNA library screening approaches were employed to identify a putative member of the highly conserved family of ATP-binding cassette transport proteins from Fasciola hepatica. At the predicted protein level, the F. hepatica sequence identified in the present study shares 43% and 36% identity with the Schistosoma mansoni SMDR2 and human MDR1 ATP-binding cassette transport sequences, respectively. Northern blot and reverse transcriptase-PCR analyses have demonstrated that expression of the F. hepatica ABC-transporter homologue is confined to immature parasites. The biochemical basis for the stage-specific expression of the ATP-binding cassette transporter homologue within F. hepatica remains to be determined.
Department of Molecular Biology, Victorian Institute of Animal Science, Attwood, Australia.
M B
MB
Reed
M
M
Panaccio
R A
RA
Strugnell
T W
TW
Spithill
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D018509:DNA, Helminth / Q000032:analysis; D005210:Fasciola hepatica / Q000235:genetics* / Q000254:growth & development*; D019450:Genes, MDR / Q000235:genetics; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D018510:RNA, Helminth / Q000032:analysis; D012550:Schistosoma mansoni / Q000737:chemistry; D016415:Sequence Alignment
D018528:ATP-Binding Cassette Transporters; D018509:DNA, Helminth; D015801:Helminth Proteins; D018510:RNA, Helminth