Enzyme activity in parotid and mandibular saliva from red kangaroos, Macropus rufus.
Parotid and mandibular saliva was obtained from red kangaroos by concurrent acetylcholine isoprenaline stimulation. Salivary proteins were separated by horizontal electrophoresis on either cellulose acetate or starch gels and assessed by specific staining techniques for 23 enzymes commonly found in mammalian tissues and body fluids. Parotid saliva was positive for acid phosphatase, alpha-amylase, carbonic anhydrase, glucose-6-phosphate dehydrogenase, sorbitol dehydrogenase and superoxide dismutase activities. Mandibular saliva was positive for alcohol dehydrogenase in addition to the above six enzymes. The kangaroo salivas lacked activity for alkaline phosphatase, beta-galactosidase and non-specific esterase which occur in saliva from some mammalian species.
School of Biological Science, University of New South Wales, Sydney, Australia.
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D000109:Acetylcholine / Q000494:pharmacology; D000135:Acid Phosphatase / Q000032:analysis; D000426:Alcohol Dehydrogenase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002256:Carbonic Anhydrases / Q000032:analysis; D018678:Cholinergic Agents / Q000494:pharmacology; D004396:Coloring Agents; D004588:Electrophoresis, Cellulose Acetate; D004592:Electrophoresis, Starch Gel; D004950:Esterases / Q000032:analysis; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D007545:Isoproterenol / Q000494:pharmacology; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis; D007614:Macropodidae / Q000378:metabolism*; D008297:Male; D008322:Mammals / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012463:Saliva / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012471:Salivary Proteins and Peptides / Q000032:analysis; D013363:Submandibular Gland / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D013482:Superoxide Dismutase / Q000032:analysis; D013566:Sympathomimetics / Q000494:pharmacology; D000516:alpha-Amylases / Q000032:analysis; D001616:beta-Galactosidase / Q000032:analysis
D018678:Cholinergic Agents; D004396:Coloring Agents; D012471:Salivary Proteins and Peptides; D013566:Sympathomimetics; D000426:Alcohol Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000516:alpha-Amylases; D001616:beta-Galactosidase; D002256:Carbonic Anhydrases; D007545:Isoproterenol; D000109:Acetylcholine