Crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY.
The histidine-containing phosphotransfer (HPt) domain at the C-terminus of the anaerobic sensor kinase ArcB has been cocrystallized with the chemotaxis response regulator CheY by a hanging-drop vapor-diffusion method. Crystals belong to space group P212121 with unit-cell dimensions a = 55.32, b = 76.29 and c = 83.89 A, with one molecule in the crystallographic asymmetric unit. The crystals diffract to 2.7 A resolution. This is the first crystallization of a protein-protein complex formed by a transmitter domain of sensor kinase and a receiver domain of response regulator in the two-component signal-transduction system.
Kato
M
M
Department of Molecular Biology, Nara Institute of Science and Technology (NAIST), 8916-5 Takayama, Ikoma, Nara 630-01, Japan.
Mizuno
T
T
Hakoshima
T
T
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000737:chemistry*; D020134:Catalytic Domain; D002633:Chemotaxis; D003460:Crystallization; D004926:Escherichia coli / Q000201:enzymology*; D029968:Escherichia coli Proteins; D006639:Histidine / Q000032:analysis*; D008565:Membrane Proteins / Q000737:chemistry*; D018377:Neurotransmitter Agents / Q000737:chemistry*; D010766:Phosphorylation; D011494:Protein Kinases; D015398:Signal Transduction / Q000502:physiology
D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D018377:Neurotransmitter Agents; D006639:Histidine; D011494:Protein Kinases; C065271:arcB protein, E coli