Batch fermentation and optimization of media for Bacillus thuringiensis.
The composition of No. II medium obtained with shaking cultivation contained three factors: nitrogen source, carbon source, and inorganic salts. The relationship between component factors (x(i)) of the media and spore numbers (y) of Bacillus thuringiensis (Bt) was demonstrated by the orthogonal-rotation-combination test. A response surface equation was formed as follows: y = 384 - 7.245x1 + 11.705x2 + 15.475x3 + 14.039x1(2) + 41.831x2(2) - 79.49x3(2) - 35.375x1x2 - 3.375x1x3 - 106.625x2x3. The results showed that this method is simple, practical, and rapid enough for selecting fermentation media for Bt. In addition, the whole course of batch fermentation was also investigated.
Fujian Agricultural University, Fuzhou, China.
X
X
Guan
J
J
Chen
Z
Z
Huang
Y
Y
Tang
R
R
Gao
D001413:Bacillus thuringiensis / Q000254:growth & development* / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D005285:Fermentation; D008962:Models, Theoretical; D013171:Spores, Bacterial / Q000254:growth & development
D003470:Culture Media