[ELISA identification of agents causing light cytopathic effect isolated during the neuropathy outbreak in Cuba, 1991].
Using as a reference the strain 44/93 isolated during the neuropathy epidemic in 1991 and characterized as a producer of a light cytopathic effect (L-CPE), it was possible the standardization of an ELISA for the fast identification of other strains with similar effect. The assay consisted in a sandwich-type ELISA where the conditions selected for each reactive (10 micrograms/mL for the coating antibody, 1 mg/mL for the antigen, and dilution 1/2,000 for the conjugate) allowed to have an adequate discrimination between the antigen and the antigen control for the reference strain used. The evaluation of a panel of reference viral strains and of other L-CPE producing strains showed a 100% of coincidence between this method and the isolation in cellular culture. The results obtained permit us to recommend the use of this assay as a more precise alternative to identify these agents.
Instituto de Medicina Tropical Pedro Kouri.
G
G
González
J
J
Laferté
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P
Más
V
V
Vázquez
R
R
Rodríguez
J
J
Míguez
D003462:Cuba / Q000453:epidemiology; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D009422:Nervous System Diseases / Q000453:epidemiology / Q000821:virology
Identificación por ELISA de agentes productores de efecto citopático ligero aislados durante la epidemia de neuropatía en Cuba, 1991.