Molecular comparison of Giardia lamblia isolates.
Giardia lamblia (also Giardia duodenalis, Giardia intestinalis) isolates have been variably divided into two or three genotypes by different investigators. We have compared the triose phosphate isomerase sequences of the three genotypes (Groups 1, 2, and 3) described by Nash and shown that Groups 1 and 2 are similar, while Group 3 is markedly different from Groups 1 and 2, indicating that Group 1/2 and Group 3 correspond to the two major genotypes identified by other investigators. We have also analysed three Chinese isolates and showed that two fit into Group 3, while the third contained a mixture of Groups 1 and 3 isolates. These results confirm the relatedness of G. lamblia isolates from throughout the world, and established the feasibility of using DNA amplification and sequence analysis for detecting mixed isolates.
Department of Cell Biology, Capital University of Medical Sciences, Beijing, People's Republic of China.
S Q
SQ
Lu
A C
AC
Baruch
R D
RD
Adam
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan / Q000032:analysis; D005838:Genotype; D005872:Giardia / Q000145:classification / Q000235:genetics; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014305:Triose-Phosphate Isomerase
D016054:DNA, Protozoan; D014305:Triose-Phosphate Isomerase