[Direct sequencing of an amplified product from a serum sample].
It is reported the nucleotide and amino acidic sequence of a great variability region in the dengue 2 virus genome, starting from the RNA of the original virus with no passage in the isolation systems. It is compared with the first strain of dengue 2 isolated during the 1981 epidemic with 4 passages in lactating mouse. Results show that the nucleotide sequence of serum and of strain A15 are the same.
Alvarez
M
M
Instituto de Medicina Tropical Pedro Kouri.
Guzmán
M G
MG
Rosario
D
D
Vázquez
S
S
Pelegrino
J L
JL
Sariol
C A
CA
Kourí
G
G
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003715:Dengue / Q000097:blood; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000097:blood*
D012367:RNA, Viral
Secuenciación directa de un producto amplificado de una muestra de suero.