Accelerator mass spectrometry as a bioanalytical tool for nutritional research.
Accelerator Mass Spectrometry is a mass spectrometric method of detecting long-lived radioisotopes without regard to their decay products or half-life. The technique is normally applied to geochronology, but is also available for bioanalytical tracing. AMS detects isotope concentrations to parts per quadrillion, quantifying labeled biochemicals to attomole levels in milligram-sized samples. Its advantages over non-isotopic and stable isotope labeling methods are reviewed and examples of analytical integrity, sensitivity, specificity, and applicability are provided.
Center for Accelerator Mass Spectrometry, Lawrence Livermore National Laboratory, Livermore, CA 94551, USA.
J S
JS
Vogel
K W
KW
Turteltaub
D000818:Animals; D002127:Calcium Isotopes / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis; D002273:Carcinogens / Q000032:analysis; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid; D003903:Deuterium / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008954:Models, Biological; D009747:Nutritional Physiological Phenomena; D010315:Particle Accelerators; D011804:Quinolines / Q000652:urine; D011849:Radioactive Tracers; D051381:Rats; D012680:Sensitivity and Specificity
D002127:Calcium Isotopes; D002247:Carbon Isotopes; D002273:Carcinogens; D011804:Quinolines; D011849:Radioactive Tracers; D003903:Deuterium; C036989:2-amino-3,4-dimethylimidazo(4,5-f)quinoline