A computer-controlled variable light attenuator for protection and autoranging of a laser-induced fluorescence detector for capillary zone electrophoresis.
Photodetectors have a limited range over which they can measure light intensities for any particular setting. The intensity of light reaching the detector can be kept within this range by using a liquid crystal variable light attenuator controlled by a computer that continuously checks the amount of light reaching the detector and adjusts the attenuation to an appropriate level. Using such a system we have constructed an intensified charge-coupled device (ICCD) camera-based detector with a dynamic range of over six orders of magnitude which is never exposed to damaging or saturating light levels.
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. yoda@biochem.okstate.edu
J M
JM
Merz
A J
AJ
Mort
D000818:Animals; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D003201:Computers; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005453:Fluorescence; D006603:Hexuronic Acids; D007834:Lasers; D009281:Naphthalenes; D010783:Photometry; D014466:Ultraviolet Rays
D002241:Carbohydrates; D006603:Hexuronic Acids; D009281:Naphthalenes; C007819:galacturonic acid; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid