Mouse cytochrome b561: cDNA cloning and expression in rat brain, mouse embryos, and human glioma cell lines.
A cDNA encoding cytochrome b561 has been isolated from a mouse brain cDNA library, which predicts a protein of 250 amino acids with a deduced Mr of 27,770. Northern blot analysis of different mouse and rat tissues revealed one major mRNA of 3300 bp, which is abundantly distributed in a number of neuroendocrine tissues. In addition, cytochrome mRNA levels in rat brain sections showed the highest distribution of cytochrome b561 in the hypothalamus, hippocampus, thalamus, and striatum, with a moderate level in the cerebral cortex, and the lowest levels in the olfactory bulb and cerebellum. Because non-neuronal cells in the central nervous system contained peptidyl alpha-amidating monooxygenase (PAM), to which cytochrome b561 donates its electrons, we used RT-PCR to document the coexpression of cytochrome b561 with PAM and dopamine beta-hydroxylase (DBH) in glioblastoma cells. Cytochrome b561 expression was detectable in the 11-day-old mouse embryo, and the level of its mRNA increased tenfold by 15 and 17 days of gestation.
Department of Anatomy and Cell Biology, Uniformed Services University of Health Sciences, Bethesda, MD 20814, USA.
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M
Srivastava
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Pollard
P J
PJ
Fleming
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004299:Dopamine beta-Hydroxylase / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005909:Glioblastoma; D005910:Glioma / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D003573:Cytochrome b Group; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C023646:cytochrome b561; D006899:Mixed Function Oxygenases; D004299:Dopamine beta-Hydroxylase; C035189:peptidylglycine monooxygenase