Studies of murine coronavirus DI RNA replication from negative-strand transcripts.
The positive-strand transcripts as well as negative-strand transcripts of mouse hepatitis virus (MHV) defective interfering (DI) RNA, when introduced into MHV-infected cells, resulted in DI RNA replication and accumulation. The leader sequence of the majority of DI RNAs that accumulated from the expression of negative-strand DI RNA transcripts with no extra non-MHV nucleotides at the 3' end switched to that of helper virus, whereas this leader sequence switching did not occur in most of the positive-strand DI RNAs that accumulated from the expressed negative-strand DI RNA transcripts with extra non-MHV nucleotides at the 3' end. These data demonstrated that the extra 4 nucleotides at the 3'-end of negative-strand DI RNA transcripts affected leader sequence switching on DI RNA, and indicated that the leader switching probably occurred during positive-strand DI RNA synthesis.
Department of Microbiology, University of Texas at Austin 78712, USA.
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Banerjee
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Makino
D000818:Animals; D002460:Cell Line; D003673:Defective Viruses / Q000235:genetics; D051379:Mice; D006517:Murine hepatitis virus / Q000235:genetics*; D020038:RNA, Spliced Leader; D012367:RNA, Viral / Q000096:biosynthesis*
D020038:RNA, Spliced Leader; D012367:RNA, Viral