On-line capillary electrophoresis-electrospray ionization mass spectrometry using a polymerized anionic surfactant.
On-line capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has been used to determine the tricyclic antidepressant drugs (imipramine, doxepin, and amitriptyline) as well as the beta-adrenergic blocker drugs (propranolol and alprenolol). A CE-ESI-MS interface linking a manually operated CE system and a Finnigan MAT-900 sector mass spectrometer (with an Analytica electrospray ionization source) has been constructed in-house and employed for this study. Although a water/methanol based capillary zone electrophoresis (CZE) buffer was initially used to determine these analytes, enhanced resolution was obtained by addition of a polymerized surfactant, i.e., poly-sodium undecylenic sulfate (poly-SUS), into the electrokinetic chromatography (EKC) buffer. When a low concentration of this poly-SUS surfactant was added to a volatile EKC buffer, these structurally similar cationic drugs were EKC separated and on-line detected by ESI-MS.
Department of Chemistry, Louisiana State University, Baton Rouge 70803, USA.
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Warner
D000319:Adrenergic beta-Antagonists / Q000032:analysis*; D000526:Alprenolol / Q000032:analysis; D000639:Amitriptyline / Q000032:analysis; D000838:Anions; D000929:Antidepressive Agents, Tricyclic / Q000032:analysis*; D002412:Cations; D004316:Doxepin / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D007099:Imipramine / Q000032:analysis; D013058:Mass Spectrometry; D011108:Polymers; D011433:Propranolol / Q000032:analysis; D013431:Sulfates; D013501:Surface-Active Agents; D014476:Undecylenic Acids
D000319:Adrenergic beta-Antagonists; D000838:Anions; D000929:Antidepressive Agents, Tricyclic; D002412:Cations; D011108:Polymers; D013431:Sulfates; D013501:Surface-Active Agents; D014476:Undecylenic Acids; D004316:Doxepin; D000639:Amitriptyline; D000526:Alprenolol; D011433:Propranolol; D007099:Imipramine