Structure of pig plasma retinol-binding protein at 1.65 A resolution.
The crystal structure of pig plasma retinol-binding protein (RBP) has been determined at 1.65 A resolution. The space group is P212121, with a = 45.81 (4), b = 53.14 (5), c = 72.97 (8) A and one protein molecule in the asymmetric unit. The structure has been solved using the molecular replacement method and refined with restrained least squares to an R factor of 0.1844 and an Rfree of 0.237 for 18 874 and 1001 independent reflections, respectively. The relatively high resolution structure of pig holoRBP has revealed some new structural details. Moreover, it has provided a description of the binding site for Cd2+, a metal ion which is required for protein crystallization. The hepta-coordination of the RBP-bound cadmium ion involves different residues of two symmetry-related RBP molecules, consistent with the participation of the cation in intermolecular interactions that in turn promote protein crystallization.
Department of Organic Chemistry, University of Padova and Biopolymer Research Center, CNR, 35131 Padova, Italy. zanotti@pdchor.chor.unipd.it
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Zanotti
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Panzalorto
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Marcato
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Malpeli
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Folli
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Berni
D000818:Animals; D001665:Binding Sites; D002104:Cadmium / Q000378:metabolism; D002845:Chromatography; D002850:Chromatography, Gel; D003460:Crystallization; D018360:Crystallography, X-Ray; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D012177:Retinol-Binding Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D054839:Retinol-Binding Proteins, Plasma; D013552:Swine; D014801:Vitamin A / Q000378:metabolism
D046911:Macromolecular Substances; D012177:Retinol-Binding Proteins; D054839:Retinol-Binding Proteins, Plasma; D002104:Cadmium; D014801:Vitamin A