Identification of shyc, a novel gene expressed in the murine developing and adult nervous system.
The embryonal carcinoma cell line P19 responds to treatment with retinoid acid by differentiation into neuronal cell types [2]. Using radioactively labeled cDNA derived from differentiating P19 cells we screened an adult mouse brain cDNA library and isolated a gene named shyc for selective hybridizing clone. The encoded protein did not reveal homology to any known protein. We used in situ hybridization on mouse embryonic and adult brain sections to study shyc expression. The developing and embryonic nervous system showed the most prominent hybridization signals. In the adult brain the olfactory pathway was marked by shyc expression.
Köster
F
F
Zentrum für Molekulare Neurobiologie, Universität Hamburg, Germany.
Schinke
B
B
Niemann
S
S
Hermans-Borgmeyer
I
I
D048868:Adaptor Proteins, Signal Transducing; D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology*; D001923:Brain Chemistry; D054278:Embryonal Carcinoma Stem Cells; D018507:Gene Expression Regulation, Developmental; D017403:In Situ Hybridization; D051379:Mice; D008969:Molecular Sequence Data; D014411:Neoplastic Stem Cells; D009419:Nerve Tissue Proteins / Q000235:genetics*
D048868:Adaptor Proteins, Signal Transducing; C115472:Cyfip1 protein, mouse; D009419:Nerve Tissue Proteins