[Nuclear matrix-lamina-intermediate filament system in PtK 2 cells].
Selective extraction, whole mount cell preparation and DGD embeddment-free section were involved in visualizing the nuclear matrix-lamina-intermediate filament system in PtK 2 cells. After extraction the anaphase chromosome residues adjoined to the cytoplasmic intermediate filament in some areas. Immunofluorescent staining showed us that the intermediate filament reacted with AE 1 and AE 3; McAb 223 could be localized specifically on the lamina while McAb C 23 could crossreact with cytoplasmic intermediate filament beside the lamina location, monoclonal antibody against lamin A (C) could also bind to chromosome residues. Antibody to 280 kD nuclear matrix protein which were positive stained in HeLa cells could not react with the nuclear matrix components of PtK 2 cells. 2-D electrophoresis demonstrated that there were some differences in the composition of the nuclear matrix-lamina-intermediate filament system of HeLa and PtK 2 cells. TdR treatment could lead to alteration of nuclear matrix proteins.
Department of Anatomy, Chinese University of Hong Kong, Shatin NT, Hong Kong.
R J
RJ
Jiao
J M
JM
Chen
Z H
ZH
Zhai
L
L
Yang
E C
EC
Chew
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology; D007382:Intermediate Filaments / Q000648:ultrastructure*; D007668:Kidney / Q000166:cytology*; D008394:Marsupialia; D008854:Microscopy, Electron; D015530:Nuclear Matrix / Q000648:ultrastructure*