Type E botulism associated with vacuum-packaged hot-smoked whitefish.
On January 16, 1997 two Germans got botulism after eating hot-smoked Canadian whitefish produced in Finland. The serum sample of one of the patients contained 6 MLD/ml of botulinum toxin. The type of toxin was identified as E by the toxin neutralization test and the botulinum neurotoxin type E (BoNT/E) gene was also amplified from the serum by polymerase chain reaction (PCR), but C. botulinum could not be isolated from the positive serum sample. The remains of the hot-smoked whitefish eaten by the patients contained botulinum toxin detected by the mouse bioassay and the BoNT/E gene as determined by PCR. C. botulinum was isolated from the fish sample and it was confirmed to be type E by the mouse bioassay and by PCR. Eleven other fish samples from the same lot did not contain botulinum toxin nor any BoNT gene. The incriminated food was processed on the 9th and 10th of January, 1997 from frozen whitefish imported to Finland from Canada. The pulsed-field gel electrophoretic pattern of the isolated C. botulinum strain resembled a reference strain of North American origin. It did not match any C. botulinum strains isolated from the Baltic sea-bottom or from the fish caught in the area indicating that the fish was contaminated by C. botulinum in Canada. The conditions resulting in toxin production could not be identified. The safety problems associated with vacuum-packaged hot-smoked fish seem to be of utmost concern and the product is one of the most important botulism food vehicles processed on an industrial scale. Temperature monitoring and the use of time-temperature indicators are to be recommended in order to ensure adequate storage temperature from processing through to consumption. Allowing the use of nitrate and nitrite together with sufficiently high NaC1 concentration in this particular product should also be considered.
Korkeala
H
H
Department of Food and Environmental Hygiene, University of Helsinki, Finland.
Stengel
G
G
Hyytiä
E
E
Vogelsang
B
B
Bohl
A
A
Wihlman
H
H
Pakkala
P
P
Hielm
S
S
D000328:Adult; D000818:Animals; D001681:Biological Assay; D001905:Botulinum Toxins / Q000097:blood / Q000506:poisoning*; D001906:Botulism / Q000175:diagnosis / Q000209:etiology* / Q000503:physiopathology; D002170:Canada; D003014:Clostridium botulinum / Q000737:chemistry / Q000145:classification*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005387:Finland; D005516:Food Microbiology; D018857:Food Packaging; D005519:Food Preservation; D005525:Food-Processing Industry; D005858:Germany; D006801:Humans; D008297:Male; D051379:Mice; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012487:Salmonidae / Q000382:microbiology*
D004269:DNA, Bacterial; D001905:Botulinum Toxins; C096326:botulinum toxin type E