Purification and properties of an active site mutant, H48Q, of human non-pancreatic secreted phospholipase A2.
Edwards
S H
SH
Division of Biochemistry and Molecular Biology, School of Biological Sciences, University of Southampton, United Kingdom.
Baker
S F
SF
Wilton
D C
DC
D019943:Amino Acid Substitution; D020134:Catalytic Domain; D002846:Chromatography, Affinity; D003001:Cloning, Molecular; D004926:Escherichia coli; D005813:Genes, Synthetic; D005973:Glutamine; D006801:Humans; D007700:Kinetics; D010741:Phospholipases A / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D054467:Phospholipases A2; D017354:Point Mutation; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
D011994:Recombinant Proteins; D005973:Glutamine; D010741:Phospholipases A; D054467:Phospholipases A2