Protein kinase C isoform levels in normal and sodium dodecyl sulphate-irritated mouse skin.
Protein kinase C (PKC) comprises a family of related phospholipid-dependent serine/threonine protein kinases. PKC is important in signal transduction, regulating cell proliferation and differentiation. Recently, it has also been suggested that PKC may play a part in the pathogenesis of contact dermatitis. However, the expression of PKC isoforms in the skin of mice with irritant contact dermatitis (ICD) has not been examined. In this study, ICD was induced in mouse skin by applying 5%, 10% and 20% sodium dodecyl sulphate (SDS) in Finn chambers on the backs of mice and fixing with surgical dressings for 24 h. Depending upon the SDS concentration, mild to strong skin irritant reactions were observed 24 h after removal of the irritant patches. The intensity of the reactions increased with the increasing concentration of SDS. PKC isoforms alpha, beta, gamma and delta were all detected in normal mouse skin by Western immunoblotting. The specificity of the PKC isoforms detected was identified further by competitive Western immunoblotting. Compared with normal mouse skin treated with double-distilled water, the levels of PKC isoforms alpha, beta, gamma and delta in the SDS-irritated mouse skin was decreased by 24.8-75.8%. These results suggest that, in SDS-ICD, mouse skin PKC isoforms alpha, beta, gamma and delta are down-regulated. The significance of this decrease is under further investigation.
Department of Dermatology, University of Illinois at Chicago, College of Medicine 60612, USA.
L F
LF
Li
V C
VC
Fiedler
R
R
Kumar
D000818:Animals; D015153:Blotting, Western; D017453:Dermatitis, Irritant / Q000201:enzymology* / Q000209:etiology; D015536:Down-Regulation; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011493:Protein Kinase C / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D013501:Surface-Active Agents
D007527:Isoenzymes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D011493:Protein Kinase C