Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein.
The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. The intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC on LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz 1H-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S). A compilation of the established structures is shown below. [structure in text]
Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, The Netherlands.
J J
JJ
van Rooijen
J P
JP
Kamerling
J F
JF
Vliegenthart
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002845:Chromatography / Q000379:methods; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000652:urine*; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D017421:Sequence Analysis; D013431:Sulfates / Q000737:chemistry; D058949:Uromodulin
D009088:Mucoproteins; D009844:Oligosaccharides; D011134:Polysaccharides; D013431:Sulfates; C548612:UMOD protein, human; D058949:Uromodulin