Sequence of the cry11Bb11 gene from Bacillus thuringiensis subsp. medellin and toxicity analysis of its encoded protein.
The nucleotide sequence of the cry11Bb1 gene from Bacillus thuringiensis subsp. medellin was determined. The corresponding protein has a deduced molecular mass of 88.2 kDa, and is 60.9% and 83% identical to the proteins Cry11Aa1 and Cry11Ba1, respectively. The Cry11Bb1 protein contains five repetitive blocks of 16 amino acids at the C terminal part. It is highly toxic to first instar laboratory reared Aedes aegypti, Anopheles albimanus and Culex quinquefasciatus larvae.
Unidad de Biotecnología y Control Biológico, Corporación para Investigaciones Biológicas, Apartado Aéreo 7378, Medellín, Colombia.
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S
Orduz
M
M
Realpe
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R
Arango
L A
LA
Murillo
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A
Delécluse
D000330:Aedes / Q000187:drug effects; D000595:Amino Acid Sequence; D000818:Animals; D000852:Anopheles / Q000187:drug effects; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000633:toxicity; D001427:Bacterial Toxins / Q000235:genetics* / Q000633:toxicity; D001483:Base Sequence; D003465:Culex / Q000187:drug effects; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D001427:Bacterial Toxins; C115468:CRY11BB protein, Bacillus thuringiensis