High-performance liquid chromatography-atmospheric-pressure chemical ionization mass spectrometry as a new tool for the determination of the mycotoxin zearalenone in food and feed.
A new method for the determination of the mycotoxin zearalenone (ZON) in food and feed, based on HPLC-MS with an atmospheric-pressure chemical ionization (APCI) interface after extraction from cereals and clean-up by either conventional solid-phase or immunoaffinity cartridge is presented. The APCI interface parameters are optimized to provide detection of ZON with maximum sensitivity after RP separation of ZON on a C18 column with acetonitrile-water (40:60, v/v) at 1 ml/min column flow without split. Using APCI-MS detection, the sensitivity of the method was improved by a factor of ca. 50 in comparison to HPLC with fluorescence detection, allowing determination of ZON down to 0.12 microgram/kg maize which is well below present threshold values. Due to the selectivity of MS detection, it also was possible to quantitatively determine ZON both in raw extracts without clean-up using a normal-size (100 mm) chromatographic column or using only a short (20 mm) chromatographic column, when a clean-up was done to minimize possible interferences.
Institute of Analytical Chemistry, Vienna University of Technology, Austria.
E
E
Rosenberg
R
R
Krska
R
R
Wissiack
V
V
Kmetov
R
R
Josephs
E
E
Razzazi
M
M
Grasserbauer
D000821:Animal Feed / Q000032:analysis*; D001274:Atmospheric Pressure; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D013696:Temperature; D015025:Zearalenone / Q000032:analysis*
D007477:Ions; D015025:Zearalenone