Cloning and characterization of cDNAs for a bovine (Bos taurus) Mx protein.
Mx proteins are GTPases that are stringently induced in cells from many vertebrates on exposure to type I interferons (IFNs), and expression of some Mx proteins potently inhibits replication of specific viruses. Two cDNAs encoding bovine Mx proteins were isolated from an endometrial phage library. The open reading frames (ORFs) of these two clones predict proteins of 654 (Mxl) and 648 (Mxl-a) residues. Both possess the tripartite GTPase domains, dynamin signature, and leucine zipper motifs conserved in all other Mx proteins identified. The bovine protein sequences show highest identity to ovine Mx (93%) and are substantially similar to human MxA (73%) and mouse Mx1 (63%). Based on differences between the two bovine clones in the coding and 3'-untranslated regions, it was concluded that they represent two alleles of one gene, and heterozygous and homozygous cattle were identified. Expression of Mx mRNA was rapidly induced in cultured bovine cells by treatment with IFN.
Ellinwood
N M
NM
Department of Physiology, Colorado State University, Fort Collins 80523, USA.
McCue
J M
JM
Gordy
P W
PW
Bowen
R A
RA
D000595:Amino Acid Sequence; D000818:Animals; D000998:Antiviral Agents / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D020558:GTP Phosphohydrolases / Q000235:genetics*; D019204:GTP-Binding Proteins; D005815:Genetic Code; D008969:Molecular Sequence Data; D064547:Myxovirus Resistance Proteins; D011506:Proteins / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
D000998:Antiviral Agents; D018076:DNA, Complementary; C579071:MX1 protein, human; C579070:Mx1 protein, mouse; D064547:Myxovirus Resistance Proteins; D011506:Proteins; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins