Lazaroid-enhanced survival of grafted dopamine neurons does not increase target innervation.
The lazaroid U-74006F enhances survival of grafted ventral mesencephalic neurons. In this study the intraocular grafting model was used and survival and outgrowth from fetal ventral mesencephalic grafts treated with U-74006F was evaluated in nigrostriatal co-grafts. Fetal lateral ganglionic eminence was implanted into the anterior eye chamber and left to mature. Fetal ventral mesencephalon was then implanted and the eyes were treated with U-74006F. The lazaroid treatment enhanced survival of tyrosine hydroxylase (TH)-positive neurons, but did not enhance TH-positive nerve fiber growth into the striatal portions of the co-grafts. However, a marked increase in nerve fiber formation was found within the ventral mesencephalic grafts. In conclusion, increased cell survival enhanced nerve fiber formation within the ventral mesencephalic portion of the co-graft and not, as expected, in the striatal part.
Björklund
L
L
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Vidal
N
N
Strömberg
I
I
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology; D016380:Brain Tissue Transplantation / Q000502:physiology*; D002452:Cell Count; D017690:Cell Transplantation / Q000502:physiology*; D004298:Dopamine / Q000502:physiology*; D005260:Female; D006085:Graft Survival / Q000187:drug effects*; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D017072:Neostriatum / Q000201:enzymology / Q000637:transplantation*; D009474:Neurons / Q000502:physiology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D011281:Pregnatrienes / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D018696:Neuroprotective Agents; D011281:Pregnatrienes; D014446:Tyrosine 3-Monooxygenase; D000110:Acetylcholinesterase; D004298:Dopamine; C053355:tirilazad