[Detection of pathogenicity factors in strains of classical enteropathogenic Escherichia coli].
OBJECTIVE
To determine the number of strains of classic enteropathogenic E. coli (EPEC) that have the eae gene, that is considered a pathogenicity factor.


METHODS
The presence of the eae gene has been evaluated on 62 EPEC strains of ten different serogroups, isolated from children with gastroenteritis.


RESULTS
Amplification of the eae gene was positive in 10 out of 62 EPEC strains analyzed (16%) corresponding to seven different serogroups.


CONCLUSIONS
The low frequency of the detection of the eae gene on EPEC strains shows the limited correlation between the pathogenicity and the serogroup of the strains and would corroborate the need to reexamine this subject prospectively in our country.
Servicio de Microbiología, Hospital de la Santa Creu i Sant Pau, Barcelona.
C
C
Frías
N
N
Margall
B
B
Mirelis
G
G
Prats
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000502:physiology; D002352:Carrier Proteins; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology; D029968:Escherichia coli Proteins; D005759:Gastroenteritis / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction; D012703:Serotyping; D014774:Virulence
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D029968:Escherichia coli Proteins; C073819:eaeA protein, E coli
Detección de factores de patogenicidad en cepas de Escherichia coli enteropatógena clásica.