Isolation of a chromosome 1 region that contributes to high blood pressure and salt sensitivity.
Linkage analyses in the spontaneously hypertensive rat (SHR) suggest that a gene involved in blood pressure regulation may be located on rat chromosome 1, in the Sa region. To confirm this possibility, we replaced a region of chromosome 1 in the Wistar-Kyoto rat (WKY) defined by the markers D1Mit3 and MTPA with the corresponding chromosome segment from SHR. Genotyping using 65 polymorphic microsatellite markers throughout the entire genome confirmed the congenic status of this new strain designated WKY. SHR-D1Mit3/Rat57. In male WKY.SHR-D1Mit3/Rat57, mean blood pressures in the daytime and in the nighttime assessed by radiotelemetry were significantly higher than those in male progenitor WKY. Moreover, salt loading significantly increased the mean blood pressure in male WKY.SHR-D1Mit3/Rat57 but not in male progenitor WKY. The present study confirmed the existence of a gene that contributes to high blood pressure and salt sensitivity in this chromosomal segment. This congenic strain represents a new animal model for fine mapping and characterization of the gene in this region involved in salt-sensitive hypertension.
1st Department of Internal Medicine, Shiga University of Medical Science, Otsu, Japan. iwai@suncuore.shiga-med.ac.jp
N
N
Iwai
Y
Y
Tsujita
M
M
Kinoshita
D000818:Animals; D020296:Animals, Congenic; D001794:Blood Pressure / Q000187:drug effects / Q000235:genetics; D002874:Chromosome Mapping; D005819:Genetic Markers; D005838:Genotype; D006973:Hypertension / Q000139:chemically induced / Q000235:genetics*; D008297:Male; D010641:Phenotype; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D012965:Sodium Chloride / Q000009:adverse effects
D005819:Genetic Markers; D012965:Sodium Chloride