Crystallization and preliminary X-ray analysis of tetanus neurotoxin C fragment.
Two crystal forms of recombinant tetanus neurotoxin C fragment have been obtained. The C fragment corresponds to the C-terminal 451 amino-acid residues of tetanus neurotoxin and is the subunit responsible for receptor binding by the toxin. Both forms belong to space group P212121. Form I has unit-cell dimensions of a = 71.3, b = 79.7, c = 94.0 A and produces thin plate crystals. Form II has unit-cell dimensions of a = 67.4, b = 79.7, c = 91.1 A and produces thick rod-shaped crystals. Diffraction data to 2.6 A have been collected from form II crystals.
Biocrystallography Laboratory, PO Box 12055, VA Medical Center, Pittsburgh, PA 15240, USA. umland@vger.niddk.nih.gov
T C
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Umland
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Wingert
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Swaminathan
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Schmidt
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Sax
D003460:Crystallization; D018360:Crystallography, X-Ray; D008666:Metalloendopeptidases / Q000737:chemistry* / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D013744:Tetanus Toxin / Q000737:chemistry* / Q000302:isolation & purification*
D010446:Peptide Fragments; D011994:Recombinant Proteins; D013744:Tetanus Toxin; C035979:tetanospasmin; D008666:Metalloendopeptidases