Induction of plasminogen in rat hippocampal pyramidal neurons by kainic acid.
Tissue plasminogen activator (tPA) is used to treat acute stroke, but tPA- and plasminogen-gene-deficient mice exhibit resistance to neurodegeneration. Thus, it is unclear whether the tPA-plasminogen system, an extracellular proteolytic cascade plays a helpful or harmful role, and whether plasminogen is induced by neurodegeneration. In the CA3, kainic acid (KA)-injection caused neuronal damage after 6 h, and almost all of the neurons were lost after 7 days. Plasminogen mRNA was strongly induced 6 h after injection, then gradually decreased, and was very weak at 2 days after injection. Plasminogen protein was expressed after 6 h and localized in abnormally shaped neurons. The in vivo expression of plasminogen was synchronous with morphological changes in neurons. These results suggest that the expression of plasminogen induced by KA-injection may disrupt of neuron-extracellular matrix interaction and thereby contribute to cell death in neurons in the hippocampus.
Department of Neurobiology, Kyoto Pharmaceutical University, Yamashina, Japan. myasuji-ind@umin.ac.jp
Y
Y
Matsuoka
Y
Y
Kitamura
T
T
Taniguchi
D000818:Animals; D000906:Antibodies; D018690:Excitatory Amino Acid Agonists; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D007608:Kainic Acid; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism; D010958:Plasminogen / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D010960:Plasminogen Activators / Q000378:metabolism; D017966:Pyramidal Cells / Q000737:chemistry / Q000502:physiology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D000906:Antibodies; D018690:Excitatory Amino Acid Agonists; D012333:RNA, Messenger; D010958:Plasminogen; D010960:Plasminogen Activators; D005341:Fibrinolysin; D007608:Kainic Acid