Determination of tylosin residues in pig tissues using high-performance liquid chromatography.
In accordance with the maximum residue limit of 100 micrograms kg-1 established by EU legislation, a simple and sensitive high-performance liquid chromatographic (HPLC) method was developed for the measurement of tylosin residues in pig tissues (fat, kidney, liver and muscle). Tylosin, a macrolide antibiotic, is extracted with water-methanol and cleaned-up by solid-phase extraction (SPE) on cation-exchange cartridges using methanol elution. Tylosin was determined by reversed-phase HPLC with UV detection at 280 nm and the mean recovery from pig tissues fortified in the range 50-200 micrograms kg-1 was 70-85%, with intra- and inter-day RSDs in the ranges 3.4-9.1 and 3.9-10.1% respectively.
Istituto di Patologia e Igiene Veterinaria, Facoltà di Medicina Veterinaria, Università degli Studi di Padova, AGRIPOLIS, Legnaro, Italy.
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De Liguoro
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Anfossi
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Angeletti
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Montesissa
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004350:Drug Residues; D005506:Food Contamination / Q000032:analysis*; D008460:Meat; D013552:Swine; D015645:Tylosin / Q000032:analysis*
D000900:Anti-Bacterial Agents; D015645:Tylosin