A program which automatically quantitates gel electrophoretic autoradiograms.
The use of a computer-coupled film scanner to measure and analyze autoradiograms of gel electropherograms is described. A program has been written which fits Gaussian curves to the complex band pattern that constitutes a density profile without the need for estimated parameters in the input. The great majority of the fits are satisfactory. This program, which is written in FORTRAN, runs on a small, inexpensive computer. Another program which approximates a Gaussian least squares fit has been run for comparison; this procedure can also be used to refine occasional unsatisfactory fits. Finally, a program has been written which sums the density profile within specified limits, so that the integrated intensities of bands due to isolated protein components may be found.
Schumaker
M F
MF
Department of Biology, University of California at San Diego, La Jolla 92093, USA.
D000465:Algorithms; D001345:Autoradiography / Q000379:methods* / Q000706:statistics & numerical data; D003627:Data Interpretation, Statistical; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods* / Q000706:statistics & numerical data; D016018:Least-Squares Analysis; D016011:Normal Distribution; D012984:Software; D012985:Software Design