Effects of a time-varying strong magnetic field on transient increase in cytosolic free Ca2+ induced by bradykinin in cultured bovine adrenal chromaffin cells.
We tested the effects of exposure to a time-varying magnetic field changing between 0.07 and 1.7 T at an interval of 3 s on transient increase in intracellular Ca2+ stimulated by bradykinin in bovine adrenal chromaffin cells. Addition of bradykinin induced the increase in intracellular Ca2+ within a few minutes. The exposure to the magnetic field perfectly suppressed the increase in intracellular Ca2+ in Ca2+-free medium. The inhibition occurred for 15 min when the maximum magnetic flux density was more than 1.4 T. These results suggest that the exposure inhibits Ca2+ release from intracellular Ca2+ stores.
Department of Physiology, School of Medicine, The University of Tokushima, Japan.
T
T
Ikehara
K H
KH
Park
H
H
Houchi
H
H
Yamaguchi
K
K
Hosokawa
M
M
Shono
K
K
Minakuchi
T
T
Tamaki
Y
Y
Kinouchi
K
K
Yoshizaki
H
H
Miyamoto
D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D019439:Chromaffin Cells / Q000737:chemistry / Q000378:metabolism*; D004574:Electromagnetic Fields; D015398:Signal Transduction / Q000187:drug effects; D013997:Time Factors
D001920:Bradykinin; D002118:Calcium