The GroEL-like protein from Campylobacter rectus: immunological characterization and interleukin-6 and -8 induction in human gingival fibroblast.
The native GroEL-like protein was purified from Campylobacter rectus, a putative periodontal pathogen, by affinity chromatography on ATP-agarose followed by high performance liquid chromatography on Superose 6. The purified 64-kDa protein (denatured form of GroEL-like protein) was immunoreactive by SDS-PAGE and Western immunoblotting with the monoclonal antibody directed against heat shock protein 60 of human origin. The native GroEL-like protein stimulated both interleukin-6 (IL-6) and IL-8 secretion by a confluent monolayer of human gingival fibroblast in their culture supernatant. During the 22-h incubation, the amounts of IL-6 and IL-8 were increased by 5.4- and 3.5-fold, respectively. These data suggested that the GroEL-like protein might be considered to be a virulence factor of C. rectus in periodontal disease.
Department of Preventive Dentistry, School of Dentistry, University of Tokushima, Japan.
D
D
Hinode
M
M
Yoshioka
S
S
Tanabe
O
O
Miki
K
K
Masuda
R
R
Nakamura
D000595:Amino Acid Sequence; D000907:Antibodies, Bacterial; D002167:Campylobacter / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D002478:Cells, Cultured; D018834:Chaperonin 60 / Q000235:genetics / Q000276:immunology* / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D005881:Gingiva / Q000187:drug effects / Q000276:immunology; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D016209:Interleukin-8 / Q000096:biosynthesis*; D008969:Molecular Sequence Data
D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D015850:Interleukin-6; D016209:Interleukin-8