Development and evaluation of an Epstein-Barr virus (EBV) immunoglobulin M enzyme-linked immunosorbent assay based on the 18-kilodalton matrix protein for diagnosis of primary EBV infection.
A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively.
Chan
K H
KH
Department of Microbiology, Queen Mary Hospital, Hong Kong.
Luo
R X
RX
Chen
H L
HL
Ng
M H
MH
Seto
W H
WH
Peiris
J S
JS
D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D036022:Capsid Proteins; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000706:statistics & numerical data; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000276:immunology*; D005069:Evaluation Studies as Topic; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008970:Molecular Weight; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D017931:DNA Primers; C041788:Epstein-Barr viral capsid antigen; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; D014763:Viral Matrix Proteins