Production of prostaglandins E1 and E2 by adult human red blood cells.
We showed that human adult red blood cells (RBCs) produce prostaglandin E1 (PGE1) and E2 (PGE2). RBCs that were mechanically stressed in the presence of extracellular Ca2+ by being injected rapidly through a fine needle produced PGE1 and PGE2 within 30 min after this mechanical stress. The amounts of PGE1 and PGE2 produced by 1 x 10(9) mechanically stressed RBCs were approximately 50 pg and 100 pg, respectively, which were determined in the cytosolic fraction from sonicated RBCs using a competitive enzyme immunoassay method. A Western blot analysis using anti-cyclooxygenase-2 antibody revealed a band at the 70-kDa position in the samples from RBCs producing PGE1 and PGE2. Treatment with 10 micrograms/mL indomethacin completely inhibited the productions of PGE1 and PGE2. The present results may indicate a new role of RBCs in microcirculation.
NTT Medical Research Institute, Kanto Teishin Hospital, Tokyo, Japan.
T
T
Oonishi
K
K
Sakashita
N
N
Ishioka
N
N
Suematsu
H
H
Shio
N
N
Uyesaka
D000328:Adult; D000527:Alprostadil / Q000096:biosynthesis*; D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism; D015232:Dinoprostone / Q000096:biosynthesis*; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007213:Indomethacin / Q000494:pharmacology; D007700:Kinetics; D013010:Sonication; D013314:Stress, Mechanical
D016861:Cyclooxygenase Inhibitors; D000527:Alprostadil; D015232:Dinoprostone; D002118:Calcium; D007213:Indomethacin