Identification of Legionella species by random amplified polymorphic DNA profiles.
Random amplified polymorphic DNA (RAPD) was used for the identification of Legionella species. Primer SK2 (5'-CGGCGGCGGCGG-3') and standardized RAPD conditions gave the technique a reproducibility of 93 to 100%, depending on the species tested. Species-specific patterns corresponding to the 42 Legionella species were consequently defined by this method; the patterns were dependent on the recognition of a core of common bands for each species. This specificity was demonstrated by testing 65 type strains and 265 environmental and clinical isolates. No serogroup-specific profiles were obtained. A number of unidentified Legionella isolates potentially corresponding to new species were clustered in four groups. RAPD analysis appears to be a rapid and reproducible technique for identification of Legionella isolates to the species level without further restriction or hybridization.
Centre National de Référence des Legionella, EA1655, Faculté de Médecine R. T. H. Laennec, 69372 Lyon Cedex 08, France. derba@cimac-res.univ-lyon1.fr
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Lo Presti
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Riffard
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Vandenesch
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Etienne
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004783:Environmental Microbiology; D005069:Evaluation Studies as Topic; D006801:Humans; D007875:Legionella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016952:Legionella pneumophila / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D007876:Legionellosis / Q000382:microbiology; D007877:Legionnaires' Disease / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D015203:Reproducibility of Results; D013045:Species Specificity
D017931:DNA Primers