Haemorrhagic septicaemia by Aeromonas salmonicida subsp. salmonicida in a black-tip reef shark (Carcharhinus melanopterus).
One black-tip reef shark (Carcharhinus melanopterus) was found dead without previous signs of disease. Major lesions consisted in cutaneous erythema, mainly at the base of the fins, focal to diffuse inflammatory lesions in gills and intestinal wall, and discrete haemorrhages in the same organs, liver and kidneys. Microcolonies of Gram-negative rods were observed in the lamina propia of gills, underneath the intestinal mucosa and randomly distributed in the renal and hepatic parenchymas. Also, emboli containing Gram-negative rods were observed in capillaries of these organs. Aeromonas salmonicida subsp. salmonicida was isolated in pure culture from gills, liver and intestine. Specific immunostaining confirmed the relationship between the isolate and lesion-associated bacteria. No previous reports on this infection in sharks have been found in the literature.
Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
V
V
Briones
A
A
Fernández
M
M
Blanco
F
F
Ramiro
M L
ML
de Vicente
J
J
García
J L
JL
Méndez
J
J
Goyache
D000333:Aeromonas; D000818:Animals; D000836:Animals, Zoo; D005393:Fish Diseases / Q000175:diagnosis*; D005880:Gills / Q000473:pathology; D016905:Gram-Negative Bacterial Infections / Q000150:complications / Q000175:diagnosis / Q000662:veterinary*; D006483:Hemorrhagic Septicemia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D007422:Intestines / Q000473:pathology; D012754:Sharks; D012867:Skin / Q000473:pathology