AbeI, a restriction endonuclease from Azotobacter beijerinckii, which recognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3'(-5/-2).
A new restriction endonuclease Abe I has beenisolated from Azotobacter beijerinckii. This enzymerecognizes the asymmetric heptanucleotide sequence 5'-CCTCAGC-3' and cleaves within it symmetrically at positions -5/-2 in the opposing strands, producing three base protruding 5'-ends.
Institute of Biotechnology, Graiciuno 8, 2028 Vilnius, Lithuania.
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Vitkute
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Maneliene
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Janulaitis
D000256:Adenoviridae / Q000235:genetics; D001395:Azotobacter / Q000201:enzymology*; D017104:Bacteriophage M13 / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D010584:Bacteriophage phi X 174 / Q000235:genetics; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; C116121:endodeoxyribonuclease AbeI; D015252:Deoxyribonucleases, Type II Site-Specific