The winged helix transcription factor Hfh2 is expressed in neural crest and spinal cord during mouse development.
The embryonic neural crest is a unique group of cells that gives rise to the peripheral nervous system as well as many other cell types. Most of the work describing the behavior and specification of these cells has been done in the avian system, a system amenable to experimental manipulations such as tissue grafting, cell transplantation and lineage tracing. This work has been greatly facilitated by the use of molecular markers of neural crest cells such as HNK-1 and slug, markers that are not yet available for the study of mouse neural crest. Here we demonstrate that Hfh2 (HNF3 forkhead homologue 2), a member of the 'winged helix' or 'forkhead' transcription factor gene family, is expressed in premigratory and migrating neural crest cells in the early mouse embryo and in motorneuron progenitors in the developing spinal cord. Using linkage analysis we have localized the Hfh2 gene to chromosome 4 at 44.91 cM from the centromere.
Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 36th and Hamilton Walk, Philadelphia, PA 19104-6058, USA.
P A
PA
Labosky
K H
KH
Kaestner
D000818:Animals; D001665:Binding Sites; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D029721:Drosophila Proteins; D051767:Early Growth Response Protein 2; D051858:Forkhead Transcription Factors; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D060850:LIM-Homeodomain Proteins; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins; D009432:Neural Crest / Q000196:embryology* / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D051767:Early Growth Response Protein 2; C497327:Egr2 protein, mouse; D051858:Forkhead Transcription Factors; C491332:Foxb1 protein, mouse; C101757:Foxd3 protein, mouse; C101121:HOXB1 homeodomain protein; D018398:Homeodomain Proteins; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C063843:insulin gene enhancer binding protein Isl-1; D051096:Proto-Oncogene Proteins c-ret; D020794:Receptor Protein-Tyrosine Kinases; C416649:Ret protein, Drosophila