cDNA cloning and expression of a novel serine protease, TLSP.
A cDNA for a putative novel serine protease, TLSP, was cloned from human hippocampus cDNA with polymerase chain reaction based strategies. The putative amino acid sequence of TLSP is similar to the trypsin-type serine proteases. TLSP mRNA is expressed in keratinocytes. Overexpressed TLSP protein in neuro2a cells was detected in culture medium.
Yoshida
S
S
Division of Structural Cell Biology, Nara Institute of Science and Technology, Japan.
Taniguchi
M
M
Suemoto
T
T
Oka
T
T
He
X
X
Shiosaka
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D006624:Hippocampus / Q000201:enzymology*; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D010957:Plasmids; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D014357:Trypsin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C114880:trypsin-like serine protease; D012697:Serine Endopeptidases; D014357:Trypsin