Genetic effects of petroleum fuels: II. Analysis of chromosome loss and hyperploidy in peripheral lymphocytes of gasoline station attendants.
Molecular cytogenetic methods were applied to investigate the effect of the occupational exposure to low concentrations of benzene and petroleum fuels on genomic stability. Twelve male gasoline station attendants (average benzene exposure of 0.32 mg/m3 as 8h TWA) and 12 age- and smoking-matched unexposed controls were selected for the study. The incidence of hyperploidy and polyploidy in peripheral lymphocytes was evaluated through in situ hybridization of interphase cells, harvested 24 hr after stimulation, with centromeric probes of chromosomes 7, 11, 18, and X. For half of the subjects, metaphases harvested 24 hr later were analyzed. The incidence of chromosome loss in vitro was determined in cytokinesis-blocked cells, harvested at 66 hr, through the hybridization of micronuclei with a pancentromeric probe. Ten thousand chromosomes (more than 200 metaphases equivalent) and 2,000 binucleated cells/person were scored for hyperploidy and micronucleus analysis, respectively. The results obtained did not show any exposure-related excess of hyperploidy or micronucleus formation. Conversely, the age of the subjects was significantly correlated with several markers of genomic instability, such as the incidence of chromosome X and chromosome 18 hyperploidy, total hyperploidy and polyploidy, and close to statistical significance with chromosome loss. Smoking habits did not appear to contribute significantly to the effects measured. The parallel analysis of hyperploidy and polyploidy in interphase nuclei in 24-hr cultures and in metaphase cells harvested 24 hr later showed basically similar incidences of aneuploid cells, indicating that no significant selection against hyperploid and polyploid types occurred during the first cell cycle in vitro.
Laboratory of Comparative Toxicology and Ecotoxicology, Istituto Superiore di Sanità, Rome, Italy.
A
A
Carere
A
A
Antoccia
D
D
Cimini
R
R
Crebelli
F
F
Degrassi
P
P
Leopardi
F
F
Marcon
A
A
Sgura
C
C
Tanzarella
A
A
Zijno
D000395:Air Pollutants, Occupational / Q000009:adverse effects* / Q000032:analysis / Q000494:pharmacology / Q000652:urine; D000782:Aneuploidy; D001554:Benzene / Q000009:adverse effects* / Q000032:analysis / Q000494:pharmacology; D002453:Cell Cycle; D002478:Cells, Cultured; D002872:Chromosome Deletion; D002877:Chromosomes, Human / Q000187:drug effects*; D005742:Gasoline / Q000009:adverse effects* / Q000633:toxicity; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007399:Interphase; D008297:Male; D008677:Metaphase; D015162:Micronucleus Tests; D016273:Occupational Exposure; D012384:Rome; D012494:Sampling Studies; D012907:Smoking / Q000453:epidemiology
D000395:Air Pollutants, Occupational; D005742:Gasoline; D001554:Benzene