Nitric oxide as a putative messenger molecule in the crayfish olfactory midbrain.
NADPH-d histochemistry was used to investigate presumptive nitric oxide synthase (NOS)-containing neurons in the crayfish olfactory midbrain. Three anatomically different types of local olfactory interneurons exhibiting NADPH-d activity were observed: two pairs of large interneurons as well as positively stained globuli cells. Branches derived from the large interneurons were confined to the ipsilateral olfactory lobe and accessory lobe, but only a few branches innervated the olfactory lobe glomeruli. Local field potential recordings on the olfactory lobe showed that administration of SNP or SIN-1 (10-4 M) into the brain had reversible inhibitory effects on electrically-evoked responses of unidentified neuronal cell populations.
Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, VA 22903, USA.
K U
KU
Johansson
D
D
Mellon
D000818:Animals; D003400:Astacoidea; D005260:Female; D007150:Immunohistochemistry; D007395:Interneurons / Q000201:enzymology; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000502:physiology*; D009249:NADP / Q000032:analysis; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000032:analysis; D009830:Olfactory Bulb / Q000166:cytology / Q000502:physiology*; D015290:Second Messenger Systems / Q000502:physiology*
D009569:Nitric Oxide; D009249:NADP; D019001:Nitric Oxide Synthase