Inhibition of malaria-infected erythrocytes by deoxyspergualin: effect on in vitro growth of malarial cultures.
The effect of deoxyspergualin (DSG) on the K1 strain of human malarial parasite Plasmodium falciparum in vitro was studied to test a possible new antimalarial chemotherapy. Hypoxanthine labeled with tritium (3H) was used to assess macromolecular synthesis. The inhibitory effects of DSG on the parasite peaked after 72 h of incubation. Parasitemia without DSG treatment was 9%, whereas at a DSG concentration of more than 156 microg/ml it was less than 1%. The amount of [3H]hypoxanthine taken up decreased with increasing DSG concentration. DNA synthesis of malarial activity decreased with increasing DSG concentration. These findings provide more evidence for the effects of DSG on this malarial parasite. As in previous in vivo studies done with DSG, the in vitro findings showed that DSG may be a new antimalarial drug.
Suzuka University of Medical Science, Department of Public Health, School of Medicine, Mie University, Mie, Japan.
Y
Y
Midorikawa
Q M
QM
Haque
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S
Nakazawa
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology*; D002478:Cells, Cultured; D016054:DNA, Protozoan / Q000096:biosynthesis; D004912:Erythrocytes / Q000187:drug effects* / Q000469:parasitology*; D006146:Guanidines / Q000494:pharmacology*; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000187:drug effects* / Q000378:metabolism; D014316:Tritium
D000962:Antimalarials; D016054:DNA, Protozoan; D006146:Guanidines; D014316:Tritium; C037258:gusperimus