Growth inhibition of experimental glioma by human interferon-beta superinduced by cationic liposomes entrapping polyinosilic:polycytidilic acid.
Intracellular production of human interferon-beta (HuIFN-beta) was enhanced in three glioma cell lines (U251-MG, U251-SP, and U251-NN) using modified superinduction with cationic liposomes containing polyinosilic:polycytidilic acid (polyI:polyC), initially given with cycloheximide, to decrease the toxicity due to polyI:polyC. Modified superinduction had a significantly (p < 0.02-0.0001) greater inhibitory effect on all three glioma cell lines, and less toxicity than superinduction. A pilot trial in experimental gliomas implanted into nude mice was also performed. Superinduction including intratumoral injection of cationic liposomes containing polyI:polyC resulted in growth inhibition of U251-NN tumors and eradication of U251-SP tumors. Weight gain in mice was not inhibited by modified superinduction. Cationic liposomes decreased drug toxicity, and may help to target tumor cells in the modified superinduction of endogenous HuIFN-beta production.
Department of Neurosurgery, Nagoya University School of Medicine.
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Bucur
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Mizuno
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Wakabayashi
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Yoshida
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage*; D002455:Cell Division / Q000187:drug effects; D003513:Cycloheximide / Q000008:administration & dosage; D004337:Drug Carriers; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000008:administration & dosage*; D016899:Interferon-beta / Q000008:administration & dosage*; D008081:Liposomes; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D011070:Poly I-C / Q000008:administration & dosage*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000473:pathology
D000970:Antineoplastic Agents; D004337:Drug Carriers; D007369:Interferon Inducers; D008081:Liposomes; D016899:Interferon-beta; D003513:Cycloheximide; D011070:Poly I-C