Dehydration of Hydrated Bilayer of Dipalmitoylphosphatidylcholine Caused by Beryllium Ion: Evidence from a Differential Scanning Calorimetry of Bilayer Phase Transition.
The effect of polyvalent metal ions Be2+, Mg2+, Ca2+, Sr2+, Ba2+, and La3+ on the phase transition behavior of hydrated bilayer of dipalmitoylphosphatidylcholine (DPPC) was investigated by differential scanning calorimetry (DSC) in relation to their ability to induce the aggregation of DPPC vesicles. The addition of the metal ions other than Be2+ provided DSC thermograms characteristic to a fully hydrated DPPC bilayer. By the addition of Be2+, the endothermic peak associated with the bilayer phase transition was shifted to that corresponding to partially dehydrated DPPC bilayer, which was reported by Kodama et al. (Biochem. Biophys. Acta 689, 567, 1982). This demonstrates that Be2+ causes dehydration of DPPC head group in hydrated bilayer and supports the speculation that the unusual property of Be2+ to induce the aggregation of PC vesicles is attributed to the destruction of repulsive hydration force due to the partial dehydration of vesicular surface. Copyright 1998 Academic Press.
Faculty of Science, Fukuoka University, Nanakuma, Jonan-ku, Fukuoka, 814-0180, Japan
H
Minami
T
Inoue