Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: identification of serogroups O26 and O111.
Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.
Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
M
M
Louie
S
S
Read
A E
AE
Simor
J
J
Holland
L
L
Louie
K
K
Ziebell
J
J
Brunton
J
J
Hii
D018829:Adhesins, Bacterial; D000293:Adolescent; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D002352:Carrier Proteins; D003092:Colitis / Q000175:diagnosis* / Q000382:microbiology*; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology*; D029968:Escherichia coli Proteins; D005243:Feces / Q000382:microbiology; D005260:Female; D006471:Gastrointestinal Hemorrhage / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012703:Serotyping; D022622:Shiga Toxin 1; D014774:Virulence / Q000235:genetics
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001427:Bacterial Toxins; D002352:Carrier Proteins; D017931:DNA Primers; D029968:Escherichia coli Proteins; D022622:Shiga Toxin 1; C073819:eaeA protein, E coli