Accessing lysozyme nucleation with a novel dynamic light scattering detector.
Dynamic light scattering was employed to investigate the behaviour of nucleating hen egg-white lysozyme solutions. For these studies we employed a novel fiber-optic based microprobe that suppresses multiple light scattering and contributions from large clusters in the spectra by backscattering detection. The time evolution of small lysozyme clusters is found to obey classical nucleation at the initial stages of the reaction.
ALV-Laser Vertiebsgesellschaft mbH, Robert-Bosch-Strasse 46, D-63225 Langen, Germany.
R
R
Peters
Y
Y
Georgalis
W
W
Saenger
D000818:Animals; D002645:Chickens; D003460:Crystallization; D004527:Egg Proteins / Q000737:chemistry; D005069:Evaluation Studies as Topic; D005336:Fiber Optic Technology; D007834:Lasers; D008027:Light; D008832:Microchemistry / Q000295:instrumentation*; D009113:Muramidase / Q000737:chemistry*; D055100:Optical Fibers; D012542:Scattering, Radiation; D013053:Spectrophotometry / Q000295:instrumentation*
D004527:Egg Proteins; D009113:Muramidase