Isolation of acidophilic methane-oxidizing bacteria from northern peat wetlands.
Acidic northern wetlands are an important source of methane, one of the gases that contributes to global warming. Methane oxidation in the surface of these acidic wetlands can reduce the methane flux to the atmosphere up to 90 percent. Here the isolation of three methanotrophic microorganisms from three boreal forest sites is reported. They are moderately acidophilic organisms and have a soluble methane monooxygenase. In contrast to the known groups of methanotrophs, 16S ribosomal DNA sequence analysis shows that they are affiliated with the acidophilic heterotrophic bacterium Beijerinckia indica subsp. indica.
Dedysh
S N
SN
Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia.
Panikov
N S
NS
Liesack
W
W
Grosskopf
R
R
Zhou
J
J
Tiedje
J M
JM
D000595:Amino Acid Sequence; D005075:Biological Evolution; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics*; D005798:Genes, Bacterial; D016946:Gram-Negative Aerobic Rods and Cocci / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism*; D008772:Methylococcaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D016415:Sequence Alignment; D012800:Siberia; D012988:Soil Microbiology; D012995:Solubility
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D010105:Oxygenases; C021555:methane monooxygenase; D008697:Methane