[Prevalence of subgroups, serotypes, and electropherotypes of human rotavirus in Cumanaá, Venezuela].
From June 1992 to May 1993, rotaviruses were detected by an immunoenzymatic assay in 159 (49.5%) of 321 children admitted to the hospital with acute diarrhea. Of the 159 cases ELISA positive, 80 samples were chosen at random to investigate subgroups and serotypes of group A human rotavirus. By the ELISA test 9 (11.3%) of the strains were subgroup I, 46 (57.5%) were subgroup II, and 25 (31.3%) could not be grouped. The serotype G1 was identified in 52 cases (65%), G2 in 11 cases (13.8%), G3 in 1 case (1.2%), and 7 cases (8.8%) showed more than one serotype. By electrophoretic analysis of viral RNA, 137 (42.7%) of the samples exhibited an RNA pattern. The long pattern (59.1%) prevailed over the short pattern (35.8%), and by coelectrophoresis 8 different electropherotypes were found throughout the period of study. These results illustrate the great variety of rotavirus strains in this region of the country.
Postgrado en Biología Aplicada, Universidad de Oriente, Núcleo de Sucre, Cumaná, Estado Sucre, Venezuela.
A J
AJ
Maldonado
J W
JW
Bastardo
D000914:Antibodies, Viral / Q000097:blood; D002648:Child; D002675:Child, Preschool; D003967:Diarrhea / Q000453:epidemiology / Q000821:virology*; D003968:Diarrhea, Infantile / Q000453:epidemiology / Q000821:virology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007223:Infant; D012367:RNA, Viral / Q000032:analysis; D012401:Rotavirus / Q000145:classification* / Q000276:immunology / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology*; D016036:Seroepidemiologic Studies; D012703:Serotyping; D014687:Venezuela / Q000453:epidemiology
D000914:Antibodies, Viral; D012367:RNA, Viral
Prevalencia de subgrupos, serotipos y electroferotipos de rotavirus humanos en Cumaná, Venezuela.