Plasmid-mediated gene transfer between insect-resident bacteria, Enterobacter cloacae, and plant-epiphytic bacteria, Erwinia herbicola, in guts of silkworm larvae.
Five strains of Enterobacter cloacae isolated from several species of plants and insects were able to grow in the guts of silkworm larvae. A much larger population of Ent. cloacae strains was detected in the insect guts and feces collected 3 and 6 days than in samples collected 1 day after feeding artificial diets contaminating these bacteria. Furthermore, insect-origin strains of Ent. cloacae were mated with a donor strain, epiphytic Erwinia herbicola, harboring RSF1010 and pBPW1::Tn7 plasmids in the insect guts by introducing these bacteria through separate artificial diets administered at different times. A number of transconjugants, Ent. cloacae strains which had acquired RSF1010 plasmid, were detected from guts and fecal samples at transfer frequencies of 10(-2) to 10(-3) per recipient. Thus, gene transfer between epiphytic Er. herbicola and insect-resident Ent. cloacae strains in the insect guts was confirmed. These findings may provide significant information about the role of "in insecta mating" in the evolution of these bacteria.
Watanabe
K
K
National Institute of Sericultural and Entomological Science, Tsukuba, Ibaraki 305-8634, Japan.
Sato
M
M
D000818:Animals; D015139:Blotting, Southern; D012831:Bombyx / Q000382:microbiology*; D015169:Colony Count, Microbial; D003227:Conjugation, Genetic; D004064:Digestive System / Q000382:microbiology; D016972:Enterobacter cloacae / Q000235:genetics* / Q000254:growth & development; D004885:Erwinia / Q000235:genetics* / Q000254:growth & development; D005243:Feces / Q000382:microbiology; D007814:Larva / Q000382:microbiology; D010944:Plants / Q000382:microbiology; D010957:Plasmids / Q000235:genetics*