3-Bromoacetylamino benzoylurea (3-BAABU), a new antimicrotubule cancericidal agent applied in cytogenetic analysis in hematology.
3-Bromoacetylamino benzoylurea (3-BAABU) is a newly synthesized antimicrotubule cancericidal compound. In the present study, we investigated the possibility of using 3-BAABU as a mitotic blocking agent for hematologic karyotyping. Treatment with 3-BAABU caused scattering of metaphase chromosomes throughout the cytoplasm both in phytohemagglutinine (PHA)-stimulated human lymphocytes and in human leukemic cells. Kinetic showed a rapid uptake of 3-BAABU by treated cells and irreversibility of its effect. Using 3-BAABU in routine procedure, a karyotype of lymphocytes from a normal male was 46, XY, with normal structure and CEM leukemic line was 85, XX, in a representative spread with abnormalities similar to reports using other blocking agents. Using 3-BAABU in spectral karyotyping, details of translocations in CEM leukemic cells were readily detected in several chromosomes, such as 7 [t(7;11)], 8 [t(8;9)], 9 [t(8;9) & t(9;19)], 11 [t(7;11)], 16 [t(16;18;20)] and 20 [t(1;20)]. 3-BAABU displayed two important characters in cytogenetics, 1) it caused dispersion of chromosomes, avoiding chance of overlap; 2) compared to the conventional mitotic blocking agent, vinblastine sulfate, 3-BAABU exhibited much gentle effect on chromosomes, thus providing more flexibility in time to perform karyotyping.
Jiang
J D
JD
Division of Neoplastic Diseases of Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
Wang
Y
Y
Janish
C A
CA
Holland
J F
JF
Bekesi
J G
JG
D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D002877:Chromosomes, Human / Q000187:drug effects / Q000648:ultrastructure*; D003582:Cytogenetics / Q000379:methods; D053938:DNA Fragmentation; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D015458:Leukemia, T-Cell; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008297:Male; D008870:Microtubules / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D014508:Urea / Q000493:pharmacokinetics / Q000494:pharmacology*
C112347:3-bromoacetylamino benzoylurea; D000970:Antineoplastic Agents; D014508:Urea