Regulation of the expression of a gene encoding beta-endoglucanase secreted by Myxococcus xanthus during growth: role of genes involved in developmental regulation.
An endoglucanase, CelA, is secreted by Myxococcus xanthus only during exponential growth. The production of this enzyme is decreased by mutations in 5 different genes (Exc +/- phenotype), three of which correspond to asg genes which regulate the production of an early cell-to-cell signal in development. Transcription of celA is decreased in two of these Exc +/- mutants, whereas a post-transcriptional step is affected in two other Exc- mutants. Thus, asg genes, in addition to regulating the onset of development, also regulate a gene (celA) that is expressed during exponential growth and that is not involved in development.
Bensmail
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L
Laboratoire de Microbiologie du Froid, IFR 61 CNRS, INSERM, Université de Rouen, Mt. St Aignan, France.
Quillet
L
L
Petit
F
F
Barray
S
S
Guespin-Michel
J F
JF
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002364:Caseins / Q000737:chemistry; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D004586:Electrophoresis; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000096:biosynthesis / Q000235:genetics; D010770:Phosphotransferases; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D002364:Caseins; C090770:EXCB protein, Salmonella typhimurium; C090769:ExcA protein, Salmonella typhimurium; C034477:RNA primers; D012333:RNA, Messenger; C026018:azocasein; D012313:RNA; D010770:Phosphotransferases; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C093119:AsgA protein, Myxococcus xanthus; C074775:CelA endoglucanase; D002480:Cellulase