Restriction map of a YAC and cosmid contig encompassing the oculopharyngeal muscular dystrophy candidate region on chromosome 14q11.2-q13.
As part of our effort to clone positionally the oculopharyngeal muscular dystrophy (OPMD) gene, we constructed a YAC contig, a cosmid contig, and an EcoRI restriction map of the OPMD candidate region. The YAC contig spans more than 2 Mb and encompasses the loci D14S283 and D14S990 and the cardiac alpha and beta myosin heavy chain genes (MYH6 and MYH7). A 700-kb cosmid contig containing the D14S990 and the myosin genes and a long-range restriction map covering the region between D14S990 and the MYH6 and MYH7 gene cluster were established. A detailed EcoRI restriction map of the cosmid contig was determined, and five putative CpG islands were identified. Based on these data, the four loci were mapped within an approximately 600-kb region with the following centromere to telomere order: D14S283, D14S990, MYH6, and MYH7. The YAC and cosmid contigs will facilitate the identification of genes lying within the OPMD candidate interval.
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Center for Research in Neuroscience, McGill University, Montreal General Hospital, 1650 Cedar Avenue, Montreal, Quebec, H3G 1A4, Canada.
Rochefort
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Brais
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Howard
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Han
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Gou
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Maciel
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The
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Larsson
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Rouleau
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D016386:Chromosome Walking; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D020451:Contig Mapping; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D009136:Muscular Dystrophies / Q000235:genetics*; D009801:Oculomotor Muscles; D010609:Pharyngeal Muscles; D015183:Restriction Mapping
D005819:Genetic Markers