Gene expression in the electrically stimulated differentiation of PC12 cells.
Cell differentiation of PC12 cells was electrically induced to grow neurites in the absence of nerve growth factor (NGF) on the electrode surface, of which potential was modulated by a rectangular wave of potential. The electric stimulation induced the c-fos expression which is essential for cell differentiation. Non-specific calcium channel blocker, lanthanum ion, inhibited the electrically induced differentiation, while NGF-induced differentiation was not suppressed. An L-type calcium channel blocker, nifedipine, also inhibited the electrically induced calcium influx and c-fos expression. Moreover, a stretch-activated (SA) channel blocker, gadolinium ion, inhibited the electrically stimulated differentiation by blocking the calcium influx, but gave no prominent effects on the potassium ion-induced differentiation. Chelerythrine, a specific protein kinase C (PKC) inhibitor, almost inhibited the cell differentiation by the electric stimulation but not by the NGF treatment. These results indicate that the alternative potential may stimulate cell differentiation through a PKC cascade.
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
K
K
Kimura
Y
Y
Yanagida
T
T
Haruyama
E
E
Kobatake
M
M
Aizawa
D000470:Alkaloids; D000818:Animals; D053119:Benzophenanthridines; D002118:Calcium / Q000378:metabolism; D002121:Calcium Channel Blockers / Q000494:pharmacology; D015220:Calcium Channels / Q000502:physiology; D002454:Cell Differentiation / Q000235:genetics*; D002455:Cell Division / Q000502:physiology; D004558:Electric Stimulation; D005682:Gadolinium / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D007811:Lanthanum / Q000494:pharmacology; D009414:Nerve Growth Factors / Q000494:pharmacology; D009543:Nifedipine / Q000494:pharmacology; D016716:PC12 Cells / Q000166:cytology*; D010617:Phenanthridines / Q000494:pharmacology; D011493:Protein Kinase C / Q000037:antagonists & inhibitors; D016760:Proto-Oncogene Proteins c-fos / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats
D000470:Alkaloids; D053119:Benzophenanthridines; D002121:Calcium Channel Blockers; D015220:Calcium Channels; D009414:Nerve Growth Factors; D010617:Phenanthridines; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D007811:Lanthanum; D005682:Gadolinium; C016299:chelerythrine; D011493:Protein Kinase C; D009543:Nifedipine; D002118:Calcium