Regulation of the expression of a gene encoding beta-endoglucanase secreted by Myxococcus xanthus during growth: role of genes involved in developmental regulation.
An endoglucanase, CelA, is secreted by Myxococcus xanthus only during exponential growth. The production of this enzyme is decreased by mutations in 5 different genes (Exc +/- phenotype), three of which correspond to asg genes which regulate the production of an early cell-to-cell signal in development. Transcription of celA is decreased in two of these Exc +/- mutants, whereas a post-transcriptional step is affected in two other Exc- mutants. Thus, asg genes, in addition to regulating the onset of development, also regulate a gene (celA) that is expressed during exponential growth and that is not involved in development.
Laboratoire de Microbiologie du Froid, IFR 61 CNRS, INSERM, Université de Rouen, Mt. St Aignan, France.
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Bensmail
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Quillet
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Petit
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Guespin-Michel
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015152:Blotting, Northern; D002364:Caseins / Q000737:chemistry; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D004586:Electrophoresis; D015964:Gene Expression Regulation, Bacterial; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016941:Myxococcus xanthus / Q000235:genetics* / Q000254:growth & development; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000096:biosynthesis / Q000235:genetics; D010770:Phosphotransferases; D012313:RNA / Q000737:chemistry; D012333:RNA, Messenger / Q000096:biosynthesis; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic / Q000235:genetics
D001426:Bacterial Proteins; D002364:Caseins; C090770:EXCB protein, Salmonella typhimurium; C090769:ExcA protein, Salmonella typhimurium; C034477:RNA primers; D012333:RNA, Messenger; C026018:azocasein; D012313:RNA; D010770:Phosphotransferases; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C093119:AsgA protein, Myxococcus xanthus; C074775:CelA endoglucanase; D002480:Cellulase