Nucleotide sequence of the gene encoding the precursor protein of pepstatin insensitive acid protease B, scytalidopepsin B, from Scytalidium lignicolum.
A chromosomal DNA of Scytalidium lignicolum was digested with Sau3AI. The digest was self-ligated and amplified by inverse PCR procedure using primers designed based on the nucleotide sequences of up- and down-stream regions of an intron present in the scytalidopepsin B gene. Analysis of the nucleotide sequence of PCR product (700 bp) showed that the enzyme is synthesized as a precursor protein consisting of the prepro- and mature enzyme regions. The deduced amino acid sequence was highly similar to those of aspergillopepsin A and recently reported endothiapepsins B and C, but quite different from those of pepstatin-insensitive bacterial acid proteases and the pepstatin-sensitive aspartic protease family.
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
N
N
Oda
Y
Y
Gotoh
H
H
Oyama
S
S
Murao
K
K
Oda
D
D
Tsuru
D000595:Amino Acid Sequence; D016282:Aspartic Acid Endopeptidases / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics*; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010436:Pepstatins / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011480:Protease Inhibitors / Q000737:chemistry; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D004792:Enzyme Precursors; D010436:Pepstatins; D011480:Protease Inhibitors; D011498:Protein Precursors; C014195:Streptomyces pepsin inhibitor; D016282:Aspartic Acid Endopeptidases; C041236:Scytalidium lignicolum acid proteases; C031375:pepstatin