[Antigenicity of fresh, cryopreserved, or liquid-medium-preserved human parathyroid adenomas].
We have studied the ability of the cryopreservation and culture techniques to reduce the antigenicity of human parathyroid tissue by suppressing HLA DR bearing cells. Antigenicity was studied with an immunoperoxidase technique applied on frozen sections. Antibody against HLA DR, CD1a, CD3, CD22, CD45RA, CD68 and H et Y antigens were used. In fresh parathyroid tissue, endothelial cells, histiocytes and interstitial dendritic cells expressed HLA DR antigens. Antigenicity of cryopreserved tissue were not altered. In cultured tissue, interstitial HLA DR bearing cells have disappeared but antigenicity of endothelial cells were not modified.
Martin
L
L
Laboratoire d'Anatomie et de Cytologie Pathologiques, Hôpital Jean Minjoz, Besançon, France.
Viennet
G
G
Racadot
E
E
Saint Hillier
Y
Y
Carbillet
J P
JP
D000236:Adenoma / Q000276:immunology*; D015703:Antigens, CD / Q000276:immunology*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002478:Cells, Cultured; D015925:Cryopreservation; D003470:Culture Media; D004727:Endothelium / Q000276:immunology; D005629:Frozen Sections; D006684:HLA-DR Antigens / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007519:Isoantigens / Q000276:immunology; D010280:Parathyroid Glands / Q000637:transplantation; D010282:Parathyroid Neoplasms / Q000276:immunology*; D011309:Preservation, Biological / Q000379:methods*; D017154:Stromal Cells / Q000276:immunology
D015703:Antigens, CD; D000951:Antigens, Neoplasm; D003470:Culture Media; D006684:HLA-DR Antigens; D007519:Isoantigens
Antigénicité des adénomes parathyroïdiens humains frais, cryopréservés et conservés en milieu liquide.