Immunohistochemical characterization of two novel monoclonal antibodies that recognize human perivascular cells of the central nervous system and macrophage subsets.
Two monoclonal antibodies to cells of monocyte/macrophage lineage were established using a human glial cell-rich fraction as the immunogen. The antibodies, named GP-1 and GP-2, were originally found to react with perivascular cells of the central nervous system. They are immunohistochemically applicable on routinely formalin-fixed, paraffin-embedded tissue sections. GP-1 binds to a lysosomal protein, and GP-2 to a carbohydrate epitope of the cell membrane and lysosomes. Among the visceral organs, GP-1 labeled blood monocytes, almost all kinds of tissue and infiltrating macrophages in both normal and diseased states, and renal tubules. GP-1 staining of tissue macrophages tends to be intensified under inflammatory conditions. GP-1 staining also suggested that perivascular cells and macrophages had different ontogeny. GP-2 immunostained monocytes, Kupffer's cells, red pulp macrophages, infiltrating macrophages and reactive microglia, but not alveolar or tingible body macrophages. Besides those macrophages, GP-2 stained mantle zone lymphocytes, some hematopoietic cells, pneumocytes and renal collecting ducts. The staining pattern of ligands on THP-1 and HL-60 neoplastic human macrophage cell lines was dissimilar to that of other macrophage markers, suggesting that they recognize unknown macrophage-related antigens.
First Department of Pathology, Gunma University School of Medicine, Maebashi, Japan. yokoo@sb.gunma-u.ac.jp
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H
Yokoo
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Sasaki
J
J
Hirato
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Nakazato
D000328:Adult; D000368:Aged; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001921:Brain / Q000276:immunology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D009000:Monocytes / Q000276:immunology; D009457:Neuroglia / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000276:immunology; D014018:Tissue Distribution; D014781:Viscera / Q000276:immunology
D000911:Antibodies, Monoclonal