In vivo 3D localized 13C spectroscopy using modified INEPT and DEPT.
The 3D localized 13C spectroscopy methods LINEPT and LODEPT, which are modifications of INEPT and DEPT, are proposed. As long as a 13C inversion pulse (180-degree pulse) is applied at 1/(4J) before the proton echo time in LINEPT and a 13C excitation pulse (90-degree pulse) is applied at 1/(2J) before the proton echo time in LODEPT, the proton echo time can be set to any value longer than 1/(2J) in LINEPT and longer than 1/J in LODEPT. As a result, the proton and the 13C pulses can be applied separately and these proton pulses can be made slice-selective pulses. These localization features of LINEPT and LODEPT were evaluated using a phantom consisting of a cylinder filled with ethanol placed inside another cylinder filled with oil, and localized ethanol spectra could be obtained. In vivo 3D localized 13C spectra from the brain of a monkey could be obtained using decoupled LINEPT, and glutamate C-4 appeared directly after the administration of glucose C-1, followed by the appearance of glutamate C-2, C-3 and glutamine C-2, C-3, C-4.
Watanabe
H
H
Toshiba Research and Development Center, Kawasaki, 210-8582, Japan. watanabe@eel.rdc.toshiba.co.jp
Ishihara
Y
Y
Okamoto
K
K
Oshio
K
K
Kanamatsu
T
T
Tsukada
Y
Y
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000295:instrumentation*; D008252:Macaca fascicularis; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation*; D019047:Phantoms, Imaging; D012680:Sensitivity and Specificity
D001786:Blood Glucose; D018698:Glutamic Acid