Evaluation of B and T lymphocytes and plasma cells in colonic mucosa from healthy dogs and from dogs with inflammatory bowel disease.
The aim of this study was to investigate the subpopulations of lymphocytes in the colonic mucosa of healthy dogs and dogs with inflammatory bowel disease (IBD). Fourteen normal dogs and 13 dogs with IBD were examined. Endoscopic biopsy specimens of colonic mucosa from each dog were stained specifically for pan T lymphocytes (CD3) and pan B lymphocytes (CD79a), and for plasma cells with methyl green pyronin (MGP) stain. Cells were counted by means of a grid and statistical analysis was performed on the data collected. B and T lymphocytes were also counted in the glandular epithelium of normal dogs and dogs with IBD and the normal and abnormal groups compared statistically. Healthy dogs had significantly lower numbers of T cells in the lamina propria and glandular epithelium and significantly lower numbers of B cells in the lamina propria. Significant group differences for plasma cells were not evident. Our results indicate that in IBD a chronic cellular immune reaction is present in the diseased gut involving increased numbers of B and T lymphocytes.
Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Small Animal Clinic, Summerhall.
J
J
Stonehewer
J W
JW
Simpson
R W
RW
Else
N
N
Macintyre
D000818:Animals; D015703:Antigens, CD / Q000302:isolation & purification*; D016175:B-Lymphocyte Subsets / Q000276:immunology; D017252:CD3 Complex / Q000302:isolation & purification*; D051925:CD79 Antigens; D003106:Colon / Q000276:immunology*; D003113:Colonoscopy; D004396:Coloring Agents; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs / Q000276:immunology*; D007111:Immunity, Cellular; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D018655:Lymphocyte Count; D011754:Pyronine; D011947:Receptors, Antigen, B-Cell / Q000302:isolation & purification*; D012016:Reference Values; D016176:T-Lymphocyte Subsets / Q000276:immunology
D015703:Antigens, CD; D017252:CD3 Complex; D051925:CD79 Antigens; D004396:Coloring Agents; D011947:Receptors, Antigen, B-Cell; D011754:Pyronine