Association of sequences in the coat protein/readthrough domain of potato mop-top virus with transmission by Spongospora subterranea.
A monofungal culture of Spongospora subterranea was unable to acquire and transmit the T isolate of potato mop-top pomovirus (PMTV-T), which has been maintained by manual transmission in the laboratory for 30 years. A recently obtained field isolate (PMTV-S) was efficiently acquired and transmitted by the same fungus culture. Sequence analysis of the readthrough (RT) protein-coding region of PMTV-S showed the presence of an additional 543 nt in the 3' half of the coding region relative to that of PMTV-T. These additional nucleotides preserved the reading frame of the RT protein and inserted 181 amino acids into the RT protein. This was confirmed by a comparison by immunoblotting of the sizes of the RT protein of PMTV-T and other recent isolates of PMTV.
Scottish Crop Research Institute, Invergowrie, Dundee, UK. breavy@scri.sari.ac.uk
B
B
Reavy
M
M
Arif
G H
GH
Cowan
L
L
Torrance
D000595:Amino Acid Sequence; D000818:Animals; D002213:Capsid / Q000737:chemistry*; D008969:Molecular Sequence Data; D009235:Myxomycetes / Q000821:virology*; D010942:Plant Viruses / Q000737:chemistry*; D011198:Solanum tuberosum / Q000821:virology*