A specific T-cell subset with CD4+/CD38- markers derived from HIV-1 carriers induces apoptosis in healthy donor-derived T-lymphocytes.
Apoptosis is an important mechanism of human immunodeficiency virus type 1 (HIV-1)-induced T-cell depletion. Our recent findings revealed mitogenic stimulation-dependent apoptosis induction in healthy donor-derived peripheral blood T-lymphocytes after adsorption with defective HIV-1 particles through acquirement by a subset of CD4+/CD38- cells of specific killer function. Based on these in vitro observations, we have extended the significance of this killing activity of CD4+/CD38- cells directly derived from HIV-1 carriers. The CD4+/CD38- cells from HIV-1-positive individuals showed significantly higher cell-killing activities than those from HIV-1-negative donors by co-culture with allogeneic resting T-cells after mitogenic stimulation. Furthermore, most of the samples induced apoptosis in a Fas-dependent manner. Thus, it is suggested that HIV-1 infection-related apoptosis is triggered by inappropriate activation of a certain resting T-cell subset, presumably due to adsorption with HIV-1 particles.
Section of Serology, Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
M
M
Kameoka
W
W
Auwanit
S
S
Suzuki
H
H
Horikoshi
N
N
Khlai-Khlam
T
T
Meguro
K
K
Yamada
Y
Y
Tanaka
K
K
Yoshihara
R B
RB
Luftig
K
K
Ikuta
D000163:Acquired Immunodeficiency Syndrome / Q000276:immunology; D000911:Antibodies, Monoclonal; D017209:Apoptosis / Q000276:immunology*; D015415:Biomarkers; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D019014:fas Receptor / Q000276:immunology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D019014:fas Receptor