[Clinical importance of the quantification of HIV-1 RNA in cerebrospinal fluid for the diagnosis of HIV encephalitis].
We evaluated prospectively the HIV-1 RNA level in CSF as a marker of HIV encephalitis diagnosis. 110 HIV-1 infected patients (mean age: 39 years; sex-ratio M/F: 94/16) were tested for HIV-1 RNA in plasma and CSF. Lumbar punctures were performed to explore cognitive deficit, seizure or fever. HIV encephalitis was diagnosed in 15 patients (14%), other CNS disease in 34 (31%), and fever without CNS disease in 61 (55%). HIV-1 RNA was detectable in 93% of the plasma and in 62% of the CSF. No significant difference was observed in CSF HIV-1 RNA between patients with or without HIV encephalitis. CSF HIV-1 RNA was correlated with plasma HIV-1 RNA (p < 0.01), CSF protein (p < 0.01) and CSF white cell counts (p < 0.01). The absence of any significant difference between patients with or without HIV encephalitis, suggests that the CSF HIV-1 RNA level is not a good marker for its diagnosis.
Service de Virologie, Hôpital Pitié-Salpêtrière, Paris, France.
P
P
Bossi
S
S
Dupré
N
N
Dupin
A
A
Coutellier
F
F
Bricaire
C
C
Lubetzki
C
C
Katlama
V
V
Calvez
D015526:AIDS Dementia Complex / Q000134:cerebrospinal fluid / Q000175:diagnosis / Q000821:virology; D000328:Adult; D015415:Biomarkers; D018792:Encephalitis, Viral / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000821:virology; D005069:Evaluation Studies as Topic; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000134:cerebrospinal fluid / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012367:RNA, Viral / Q000097:blood / Q000134:cerebrospinal fluid*; D019562:Viral Load
D015415:Biomarkers; D012367:RNA, Viral
Intérêt clinique de la quantification de l'ARN VIH-1 dans le liquide céphalo-rachidien pour le diagnostic des encéphalites VIH.