Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism.
A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates.
Rosner
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A
Department of Virology, ARO, The Volcani Center, Bet Dagan, Israel. vpsarasp@volcani.agri.gov.il
Maslenin
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L
Spiegel
S
S
D019179:Ilarvirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D014158:Transcription, Genetic