A single-column amino acid analysis method which resolves hexosamines and several cysteine derivatives.
A single-column amino acid analysis method is presented for use in structural studies of glycoproteins. The system gives excellent resolution of glucosamine, galactosamine, cysteic acid, CM-cysteine, AE-cysteine, the internal standard norleucine, and all amino acids normally present in protein hydrolysates.
Division of Clinical Immunology and Allergy, Montreal General Hospital, Quebec, Canada.
M
M
Fauconnet
J
J
Rochemont
D000596:Amino Acids / Q000032:analysis*; D002021:Buffers; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods; D003545:Cysteine / Q000032:analysis; D006023:Glycoproteins / Q000737:chemistry; D006595:Hexosamines / Q000032:analysis; D007202:Indicators and Reagents
D000596:Amino Acids; D002021:Buffers; D006023:Glycoproteins; D006595:Hexosamines; D007202:Indicators and Reagents; D003545:Cysteine