[A monoclonal antibody-based study of the antigenic interrelations of typical and R forms of Vibrio cholerae].
Monoclonal antibodies to surface determinants of V. cholerae R forms (R-McA) were obtained. R-McA and monoclonal antibodies to lipopolysaccharide (LPS) of V. cholerae S forms (S-McA) were used to show that the LPS of deeply altered vibrios, agglutinating only with RO serum, completely lost its O-side chain. Some common O determinants on the basis of S-McA were detected in typical cultures of V. cholerae O1 and RO vibrios which agglutinated to 1/4 T with O serum and, in low titers, with RO serum. V. cholerae O1 were not capable of specifically binding with R-McA. Not all R strains under study were identified with the use of available R-McA due to essential differences of their terminal monosaccharides responsible for serological specificity.
Research Institute for Plague Control, Rostov-on-Don, Russia.
L P
LP
Alekseeva
I Ia
IIa
Cherepakhina
O I
OI
Sal'nikova
O S
OS
Burlakova
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D000954:Antigens, Surface / Q000032:analysis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D011815:R Factors; D014734:Vibrio cholerae / Q000276:immunology*
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D000939:Epitopes
Izuchenie antigennykh vzaimosviazeĭ tipichnykh i R-form kholernykh vibrionov na osnove monoklonal'nykh antitel.