Urinary metabolites of daidzin orally administered in rats.
In a study on the metabolism of flavonoids, the isoflavone glycoside daidzin was orally administered to rats. Urine samples were collected and treated with beta-glucuronidase and arylsulfatase. Aglycone daidzein (M3) and other three metabolites, 3',4',7-trihydroxyisoflavone (M1), 4',7-dihydroxyisoflavanone (M2) and 4',7-dihydroxyisoflavan (M4) were isolated from the urine following treatment with enzymes. The structures of M1, M2 and M4 were determined on the basis of chemical and spectral data.
Tohoku College of Pharmacy, Sendai, Miyagi, Japan.
T
T
Yasuda
K
K
Ohsawa
D000284:Administration, Oral; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism / Q000494:pharmacology; D002839:Chromans / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D060754:Equol; D005966:Glucuronidase / Q000378:metabolism / Q000494:pharmacology; D007529:Isoflavones / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
C007610:4',7-dihydroxy-3,4-dihydroisoflavone; D002839:Chromans; D007529:Isoflavones; C013908:daidzin; D060754:Equol; C004742:daidzein; D001192:Arylsulfatases; D005966:Glucuronidase; C061213:3',4',7-trihydroxyisoflavone