Three-dimensional appearance of Langerhans cells in human gingival epithelium as revealed by confocal laser scanning microscopy.
Detailed three-dimensional morphological information of the cell without distortion due to thin sectioning can be obtained from thick sections using a confocal laser scanning microscope. Here that microscope was used to evaluate human gingival Langerhans cells stained with anti-CD1a antibody. Optical sectioning and reconstruction by laser scanning microscopy revealed not only three-dimensional aspects of Langerhans cells but also spatial information on the distribution of their dendritic processes towards the gingival surface. The orientated configuration of those processes may reflect the reaction of the gingival Langerhans cell to the surrounding moist environment containing antigenic stimuli originating in saliva.
Ito
H
H
Department of Oral Pathology, Hiroshima University School of Dentistry, Japan.
Takekoshi
T
T
Miyauchi
M
M
Ogawa
I
I
Takata
T
T
Nikai
H
H
Takemoto
K
K
D000906:Antibodies; D000941:Antigens / Q000276:immunology; D018949:Antigens, CD1 / Q000276:immunology; D048429:Cell Size; D004396:Coloring Agents; D004847:Epithelial Cells / Q000166:cytology; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007801:Langerhans Cells / Q000166:cytology*; D018613:Microscopy, Confocal; D012463:Saliva / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D018949:Antigens, CD1; D004396:Coloring Agents