Preparative gel electrophoresis: detection, excision, and elution of protein bands from unstained gels.
Methods are described for localizing proteins in unstained gels and accurately excising the regions containing them. A gel elutor, which is all glass with Lucite fittings, is also described. The elutor removes proteins from gels with a high yield and concentrates the protein in a small volume. The elutor is simple and very easy to use. A way is presented for avoiding the oxidation of methionine and cysteine during preparative electrophoresis.
Mardian
J K
JK
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA.
Isenberg
I
I
D019342:Acetic Acid; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation / Q000379:methods*; D005656:Fungal Proteins / Q000302:isolation & purification; D006657:Histones / Q000302:isolation & purification; D008163:Luminescent Measurements; D017830:Octoxynol; D010084:Oxidation-Reduction; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D014508:Urea
D005656:Fungal Proteins; D006657:Histones; D011506:Proteins; D014508:Urea; D017830:Octoxynol; D019342:Acetic Acid