[Expression of nm23/NDPK, integrin, type IV collagenase and uPA in transplanted tumors with various metastatic potential in nude mice and surgical specimens].
OBJECTIVE
To observe the relationship between the metastasis of human lung carcinoma and the expressions of nm23/NDPK, integrins, type IV collagenase and uPA.


METHODS
By ABC immunohistochemical staining, we observed the expressions of nm23/NDPK, integrins, type IV collagenase and uPA in four strains of subcutaneous transplanted tumors and 87 surgical specimens for human lung carcinoma. The metastatic potential of four xenografts varied from low to high among PLA-801C, Anip973, PLA-801D, PLA-801DL. The effects of beta 1 antibody and the RGD peptide on the PLA-801DL xenograft were studied in the nude mice.


RESULTS
High expression of nm23/NDPK was observed in the four xenografts and specimens from the 87 patients. No association was found between expression of nm23/NDPK and lymph node metastasis. Low expressions of beta 1 subfamily integrins were observed in the four xenografts. beta 1 subunit antibody and RGD peptide had no effect on metastasis of PLA-801DL xenograft. The expressions of type IV collagenase were positively correlated with their metastatic capacity in the four xenografts and surgical specimens from the 87 patients. Anip973 and PLA-801D showed positive staining to uPA, while PLA-801DL and PLA-801C were negative.


CONCLUSIONS
The expression of type IV collagenase is positively correlated with the metastatic capacity of lung carcinoma. Although high expression of nm23/NDPK, low expression of integrins, and various expression of uPA were observed, these were not correlated with metastatic capacity of lung carcinoma.
Department of Laboratory Animal Science, Third Military Medical College, Chongqing.
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H
Wei
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Wu
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Zhou
D000818:Animals; D017364:Collagenases / Q000096:biosynthesis*; D015870:Gene Expression; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D016023:Integrins / Q000096:biosynthesis*; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009368:Neoplasm Transplantation; D009701:Nucleoside-Diphosphate Kinase / Q000096:biosynthesis; D014157:Transcription Factors / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis
D016023:Integrins; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D014568:Urokinase-Type Plasminogen Activator; D017364:Collagenases; D020559:Monomeric GTP-Binding Proteins