Efferent projection from the rostral ventrolateral medulla to the area postrema in rats.
The rostral ventrolateral medulla (RVLM) is a region of the brain primarily involved in cardiovascular control. It receives information from several areas of the brainstem, among which the area postrema (AP) and the nucleus of the solitary tract (NTS). The medial subnuclei of the solitary tract (TS) project towards the RVLM, providing cardiopulmonary information, and the AP serves information about circulatory hormones. Although the efferent pathways are well known, it is not the case for the connections from the RVLM towards the AP and the NTS. The present study was designed to examine the efferent connections from the RVLM onto the dorsal structures of the medulla: quantitatively by means of anatomical techniques, and functionally by means of electrophysiological techniques. Morphologically, Biocytin or Biotinylated dextran amine microinjections into the RVLM were followed by labelling of many fibres running towards the bulbar dorsomedial structures, with some pathways lying in the AP itself, or located in its caudal vicinity. Conversely, when microinjections of Fast Blue (FB) were made into the AP, FB-labelled cells could be observed within the RVLM. Electrophysiologically, single shock stimulation carried on AP allowed identification of axonal fibres issuing from somata located into the cardiovascular neuronal pool in the RVLM. From these results, we can assume: (1) the existence of dense efferent projection from RVLM to aspects of the dorsal vagal complex, including the AP and, among this dense projection, (2) the existence of some fibres terminating in, or crossing through the AP, and identified as conveying baroreceptor-related information, in the rat.
Département de Physiologie et Neurophysiologie (ESA CNRS 6032), Faculté des Sciences et Techniques Saint-Jérome, Marseille, France.
A
A
Frugière
E
E
Nunez
R
R
Pasaro
S
S
Gaytan
J C
JC
Barillot
D000578:Amidines; D000818:Animals; D001369:Axons / Q000502:physiology; D001710:Biotin / Q000031:analogs & derivatives; D001931:Brain Mapping; D002552:Cerebral Ventricles / Q000502:physiology*; D003911:Dextrans; D004525:Efferent Pathways / Q000502:physiology; D004594:Electrophysiology; D005260:Female; D005456:Fluorescent Dyes; D008239:Lysine / Q000031:analogs & derivatives; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D051381:Rats / Q000502:physiology*; D017208:Rats, Wistar; D009435:Synaptic Transmission / Q000502:physiology*
D000578:Amidines; D003911:Dextrans; D005456:Fluorescent Dyes; C076397:biotinylated dextran amine; C031455:diamidino compound 253-50; D001710:Biotin; C013411:biocytin; D008239:Lysine