Crystallization and preliminary X-ray analysis of a member of a new family of pectate lyases, PeIL from Erwinia chrysanthemi.
PeIL, a pectate lyase (E.C. 4.2.2.9) from E. chrysanthemi 3937 that is not homologous to the lyases with known structures, has been purified and crystallized by the hanging-drop method using a variety of organic solvents as precipitant. Elongated lathes grown from poly(ethylene glycol) plus isopropanol belong to the space group P212121 with cell dimensions a = 55.5, b = 58.2, c = 16.4 A with a single molecule in the asymmetric unit. Although complete data sets have been collected to 2.3 A resolution, these crystals diffract to at least 1.9 A resolution and are suitable for structure determination. Chunky plates grown using other organic solvents as the precipitant diffracted to 3 A resolution and were partially characterized as a second orthorhombic crystal form with space group P21212 and cell dimensions a = 119.1, b = 140.5 and c = 105.4 A, suggesting four molecules in the asymmetric unit.
Laboratoire de Génétique Moléculaire des Microorganismes, UMR CNRS 5577, INSA Bâtiment 406, 20 avenue Albert Einstein, 69621 Villeurbanne CEDEX, France.
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Shevchik
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Scott
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Mayans
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Jenkins
D003460:Crystallization; D003625:Data Collection; D016974:Dickeya chrysanthemi / Q000201:enzymology*; D005810:Multigene Family; D011133:Polysaccharide-Lyases / Q000737:chemistry* / Q000235:genetics; D014961:X-Ray Diffraction
D011133:Polysaccharide-Lyases; C037051:pectate disaccharide-lyase