Automatic collector of expired 14CO2 for liquid scintillation counting.
A filtration technique was employed to trap 14CO2 continuously for liquid scintillation counting. Devices for delivering scintillator and ethanolamine solutions were combined symmetrically with two fritted-glass aspirators for alternating operation. The collector was regulated by a fraction collector timer. Trial and animal tests indicated that the described method was efficient, reliable, and more convenient for frequent collection over long periods than alternative methods. The automatic collector was used for metabolic studies of [1-14C] arachidonic acid in rats kept in metabolic cages and the results were processed by multicompartmental analysis.
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.
A C
AC
Richards
F A
FA
Shamgar
F D
FD
Collins
D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000032:analysis* / Q000302:isolation & purification / Q000502:physiology; D002250:Carbon Radioisotopes; D019856:Ethanolamine; D008954:Models, Biological; D051381:Rats; D012119:Respiration; D012588:Scintillation Counting / Q000295:instrumentation / Q000379:methods*
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D016718:Arachidonic Acid; D019856:Ethanolamine