Ribosomal RNA genes of Phaseolus coccineus. IV. Structure and comparative-analysis of the intergenic spacer: possible involvement of some nucleotides in the transcription control of coding sequences. Sequence of the intergenic spacer of ribosomal genes.
The sequence of the intergenic spacer (IGS) of Phaseolus coccineus is determined. The IGS contains three distinct regions: Region A, constant in length; Region B, heterogeneous in length among genes, including two very similar segments 162 and 177 bp long, repeated two and nine times respectively in the investigated clone; Region C, constant in length, comprising five islands. The putative promoters and the sites of termination, processing and methylation are detected by a comparison with other plant systems.
Marrocco
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R
Dept. of Agrobiology and Agrochemistry, Tuscia University Viterbo, Italy.
Castiglione
M R
MR
Ruberti
F
F
Maggini
F
F
Avanzi
S
S
D000595:Amino Acid Sequence; D001482:Base Composition; D001483:Base Sequence; D018744:DNA, Plant / Q000737:chemistry / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000302:isolation & purification; D007887:Fabaceae / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D020459:Genes, rRNA / Q000235:genetics*; D008969:Molecular Sequence Data; D010946:Plants, Medicinal; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D013728:Terminator Regions, Genetic; D014158:Transcription, Genetic
D018744:DNA, Plant; D004275:DNA, Ribosomal