A secretory cellulose-binding protein cDNA cloned from the root-knot nematode (Meloidogyne incognita).
A cDNA encoding a secretory cellulose-binding protein was cloned from the root-knot nematode (Meloidogyne incognita) with RNA fingerprinting. The putative full-length cDNA, named Mi-cpb-1, encoded a 203 amino acid protein containing an N-terminal secretion signal peptide. The C-terminal sequence of the putative MI-CBP-1 was similar to a bacterial-type cellulose-binding domain, whereas the N-terminal sequence did not show significant similarity to any proteins in data bases. Recombinant MI-CBP-1 lacked cellulase activity, but bound to cellulose and plant cell walls. In Southern blot hybridization, Mi-cbp-1 hybridized with genomic DNA from M. incognita, M. arenaria, and M. javanica, but not M. hapla, Heterodera glycines, or Caenorhabditis elegans. Polyclonal antibodies raised against recombinant MI-CBP-1 strongly labeled secretory granules in subventral gland cells of second-stage juveniles in indirect immunofluorescence microscopy. Enzyme-linked immunosorbent assay detection of MI-CBP-1 in stylet secretions of second-stage juveniles with the polyclonal antibodies indicated MI-CBP-1 could be secreted through the nematodes' stylet, suggesting that the cellulose-binding protein may have a role in pathogenesis.
Department of Plant Pathology, University of Georgia, Athens 30602-7274, USA.
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Ding
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Shields
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Allen
R S
RS
Hussey
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics*; D019084:Fluorescent Antibody Technique, Indirect; D044643:Glucan 1,4-beta-Glucosidase; D015801:Helminth Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000469:parasitology; D017386:Sequence Homology, Amino Acid; D014431:Tylenchoidea / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity; D001617:beta-Glucosidase / Q000378:metabolism
D002352:Carrier Proteins; D017931:DNA Primers; D018076:DNA, Complementary; D018509:DNA, Helminth; D015801:Helminth Proteins; D002482:Cellulose; D001617:beta-Glucosidase; D044643:Glucan 1,4-beta-Glucosidase