Sodium dodecyl sulfate-capillary electrophoresis of proteins in a sieving matrix utilizing two-spectral channel laser-induced fluorescence detection.
We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.
Department of Chemistry, University of Alberta, Edmonton, Canada.
D B
DB
Craig
R M
RM
Polakowski
E
E
Arriaga
J C
JC
Wong
H
H
Ahmadzadeh
C
C
Stathakis
N J
NJ
Dovichi
D003207:Conalbumin / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D019793:Fluorescein; D005452:Fluoresceins; D005453:Fluorescence; D005456:Fluorescent Dyes; D005663:Furans; D007834:Lasers; D008970:Molecular Weight; D010047:Ovalbumin / Q000032:analysis; D011108:Polymers; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D011804:Quinolines; D012967:Sodium Dodecyl Sulfate; D013050:Spectrometry, Fluorescence; D014362:Trypsinogen / Q000032:analysis
C115825:6-(fluorescein-5-carboxamido)hexanoic acid succimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D005663:Furans; D011108:Polymers; D011506:Proteins; D011804:Quinolines; C063756:3-(2-furoyl)quinoline-2-carbaldehyde; D003207:Conalbumin; D012967:Sodium Dodecyl Sulfate; D014362:Trypsinogen; D010047:Ovalbumin; D019793:Fluorescein