Evaluation of a commercial ELISA test for the detection of allergen-specific IgE antibodies in atopic dogs.
A commercial enzyme-linked immunosorbent assay (ELISA) designed to detect allergen-specific immunoglobulin (Ig)E antibodies were evaluated in 36 atopic dogs and in 12 normal dogs. The test showed a sensitivity of 72.23% and a specificity of 41.6%. Positive and negative predictive values were 76.47 and 35.71% respectively. Correlation between the ELISA kit results and intradermal skin testing varied depending on the allergen and ranged from 47.1 to 80.4%, although positive correlation (i.e. allergens positive in both tests) ranged rom 2.7 to 19.4%. In conclusion, this serological test gave both false positive and false negative results. Sensitivity, specificity and predictive values indicate that this ELISA may not be useful in canine atopy. Although correlation studies were hampered by the impossibility of using the same allergenic extracts, the correlation observed between intradermal and serological testing indicates that results from both tests are not interchangeable.
Department of Veterinary Clinical Pathology, Faculty of Veterinary Medicine, University of Córdoba, Spain.
P J
PJ
Ginel
C
C
Riaño
R
R
Lucena
D000485:Allergens / Q000276:immunology*; D000818:Animals; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis / Q000276:immunology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D007073:Immunoglobulin E / Q000097:blood*; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012016:Reference Values; D012680:Sensitivity and Specificity
D000485:Allergens; D011933:Reagent Kits, Diagnostic; D007073:Immunoglobulin E