Role of farnesyltransferase in ABA regulation of guard cell anion channels and plant water loss.
Desiccation of plants during drought can be detrimental to agricultural production. The phytohormone abscisic acid (ABA) reduces water loss by triggering stomatal pore closure in leaves, a process requiring ion-channel modulation by cytoplasmic proteins. Deletion of the Arabidopsis farnesyltransferase gene ERA1 or application of farnesyltransferase inhibitors resulted in ABA hypersensitivity of guard cell anion-channel activation and of stomatal closing. ERA1 was expressed in guard cells. Double-mutant analyses of era1 with the ABA-insensitive mutants abi1 and abi2 showed that era1 suppresses the ABA-insensitive phenotypes. Moreover, era1 plants exhibited a reduction in transpirational water loss during drought treatment.
Department of Biology and Center for Molecular Genetics, University of California, San Diego, La Jolla, CA 92093-0116, USA.
Z M
ZM
Pei
M
M
Ghassemian
C M
CM
Kwak
P
P
McCourt
J I
JI
Schroeder
D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D019883:Alkyl and Aryl Transferases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000838:Anions; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005204:Farnesol / Q000031:analogs & derivatives / Q000494:pharmacology; D017353:Gene Deletion; D015870:Gene Expression; D017343:Genes, Plant; D007473:Ion Channels / Q000378:metabolism*; D009154:Mutation; D063065:Organophosphonates / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011090:Polyenes / Q000494:pharmacology; D053284:Polyunsaturated Alkamides; D017368:Protein Prenylation; D015398:Signal Transduction; D014867:Water / Q000378:metabolism*
C080570:(alpha-hydroxyfarnesyl)phosphonic acid; D000838:Anions; D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D007473:Ion Channels; D063065:Organophosphonates; D011090:Polyenes; D053284:Polyunsaturated Alkamides; D014867:Water; D005204:Farnesol; D000040:Abscisic Acid; D019883:Alkyl and Aryl Transferases; C064911:p21(ras) farnesyl-protein transferase; C087434:ABI1 protein, Arabidopsis; C106443:ABI2 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; C054474:manumycin