Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains.
Restriction fragment length polymorphism analysis of chromosomal DNA from 22 strains of Toxoplasma gondii were characterised using SalI and PstI restriction endonucleases and the TGR1E specific repetitive sequence as a probe. Two virulent strains, RH and P-CZ, had previously been isolated from humans, the remaining 20 strains were isolated from animals in the Czech Republic in 1994 and 1995. Among the 20 recently isolated strains, 19 belonged to an avirulent lineage and only one strain from the wild cat Felis silvestris belonged to a virulent lineage.
Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. zdposp@dior.ics.muni.cz
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D000818:Animals; D001717:Birds; D002335:Carnivora; D002415:Cats; D002522:Chlorocebus aethiops; D016000:Cluster Analysis; D018153:Czech Republic; D016054:DNA, Protozoan / Q000032:analysis; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D005819:Genetic Markers; D006801:Humans; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits; D012377:Rodentia; D013047:Specific Pathogen-Free Organisms; D014122:Toxoplasma / Q000235:genetics* / Q000472:pathogenicity*; D014709:Vero Cells; D014774:Virulence
D016054:DNA, Protozoan; D005819:Genetic Markers