Ultrasonic evaluation for the time of ovulation in ewes treated with norgestomet and norgestomet followed by pregnant mare's serum gonadotropin.
Progestogens and follicular stimulants have proved reasonably successful for estrus synchronization, but time of ovulation relative to removal of the progestogen is not clearly established. We monitored time of ovulation in ewes following synchronized estrus. Ovaries of 40 Dorset and Rambouillet x Dorset ewes were evaluated during the spring and fall (20/replicate). Ewes were randomly assigned to one of two treatment groups (n = 20/group): implant-only (I) ewes received a norgestomet implant for 10 d; and implant + PMSG (PI) ewes received a norgestomet implant for 10 d with an i.m. injection of 500 IU of PMSG at implant removal. Time of estrus onset was detected with the HeatWatch Estrus Detection System. Following the initiation of estrus, ovaries were monitored via rectal ultrasonography every 6 h to determine the interval from implant removal to ovulation (OVUL), and onset of estrus to ovulation (INT). Four I and PI ewes that lost implants before scheduled removal on d 10 were removed from the study. Estrus was detected in 13 of 16 (81%) I and 14 of 16 (88%) PI ewes within 108 h after implant removal. The interval from implant removal to estrus (EST), INT, and OVUL were not affected by replicate. The INT was not different in I (33.8 h) and PI (35.0 h) ewes. Mean OVUL was greater (P < .01) in I (79.8 h) than in PI ewes (68.6 h), and mean EST was also greater (P < .01) for I (46.0 h) than for PI ewes (32.6 h). The present data indicate that ovulation occurs on average 70 to 80 h after implant removal in ewes treated with norgestomet, and PMSG reduces the interval from implant removal to ovulation.
Department of Animal Science, Oklahoma State University, Stillwater 74078-6051, USA.
B E
BE
Cardwell
G Q
GQ
Fitch
R D
RD
Geisert
D000818:Animals; D004343:Drug Implants; D004973:Estrus Synchronization / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006064:Gonadotropins, Equine / Q000008:administration & dosage / Q000494:pharmacology*; D007315:Insemination, Artificial / Q000662:veterinary; D008297:Male; D010053:Ovary / Q000000981:diagnostic imaging; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D010061:Ovulation Detection / Q000662:veterinary; D011282:Pregnenediones / Q000008:administration & dosage / Q000494:pharmacology*; D045167:Progesterone Congeners / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D012756:Sheep / Q000502:physiology*; D013997:Time Factors; D014463:Ultrasonography
D004343:Drug Implants; D006064:Gonadotropins, Equine; D011282:Pregnenediones; D045167:Progesterone Congeners; C100284:norgestomet