Immunohistochemical analysis of insulin-like growth factor-binding proteins -1, -2, and -3 in implantation sites of the mouse.
OBJECTIVE
Our purpose was to analyze potential interactions between the embryo and the maternal endometrial interface in vivo by analyzing immunolocalization of insulin-like growth factor-binding proteins (IGFBPs) -1, -2, and -3 in implantation sites of the mouse.


METHODS
Six-week-old B6D2F1 female mice underwent superovulation followed by mating and sacrifice at timed intervals. Formalin-fixed paraffin-embedded tissue was used for avidin-biotin immunocytochemical localization of IGFBPs utilizing standard methodology.


RESULTS
Immunostaining at 1.5 days post coitum revealed light staining in the epithelial glandular cells and faint staining in decidual stroma for both IGFBP-1 and IGFBP-2. At 7.5-10.5 days post coitum, there was moderate-dense immunostaining in the decidualized stromal cells at the implantation site for all three IGFBPs, whereas light immunostaining was seen in nonimplantation site decidua.


CONCLUSIONS
Compartmentalization of immunostaining for IGFBP-1, -2, and -3 within decidualized stroma suggests that these proteins may be regulated by trophoblastic and/or embryonic signals.
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, New York, USA.
M A
MA
Damario
H C
HC
Liu
C A
CA
Mele
M G
MG
Horenstein
Z
Z
Rosenwaks
D015100:3,3'-Diaminobenzidine / Q000737:chemistry; D000818:Animals; D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D007106:Immune Sera / Q000096:biosynthesis / Q000276:immunology; D007150:Immunohistochemistry; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000032:analysis / Q000502:physiology*; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000032:analysis / Q000502:physiology*; D018972:Insulin-Like Growth Factor Binding Protein 3 / Q000032:analysis / Q000502:physiology*; D008297:Male; D051379:Mice; D011247:Pregnancy; D011817:Rabbits; D017154:Stromal Cells / Q000166:cytology; D013997:Time Factors; D014599:Uterus / Q000737:chemistry* / Q000166:cytology
D007106:Immune Sera; D018970:Insulin-Like Growth Factor Binding Protein 1; D018971:Insulin-Like Growth Factor Binding Protein 2; D018972:Insulin-Like Growth Factor Binding Protein 3; D001360:Avidin; D015100:3,3'-Diaminobenzidine; D001710:Biotin