Purification, characterization, and antifungal activity of chitinase from Fusarium chlamydosporum, a mycoparasite to groundnut rust, Puccinia arachidis.
Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Fusarium chlamydosporum and purified by ion-exchange chromatography and gel filtration. The molecular mass of purified chitinase was 40 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Chitinase was optimally active at a pH of 5 and stable from pH 4 to 6 and up to 40 degrees C. Among the metals and inhibitors tested, mercuric chloride completely inhibited the enzyme activity. The activity of chitinase was high on colloidal and pure chitin. The purified chitinase inhibited the germination of uredospores of Puccinia arachidis and also lysed the walls of uredospores and germ tubes. The results from these experiments indicated that chitinase of F. chlamydosporum plays an important role in the biocontrol of groundnut rust.
Centre for Advanced Studies in Botany, University of Madras, India.
N
N
Mathivanan
V
V
Kabilan
K
K
Murugesan
D000935:Antifungal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D010367:Arachis / Q000382:microbiology*; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000302:isolation & purification* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005656:Fungal Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D005658:Fungi / Q000187:drug effects; D005670:Fusarium / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D013170:Spores / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
D000935:Antifungal Agents; D004791:Enzyme Inhibitors; D005656:Fungal Proteins; D002686:Chitin; D002688:Chitinases