The adc locus, which affects competence for genetic transformation in Streptococcus pneumoniae, encodes an ABC transporter with a putative lipoprotein homologous to a family of streptococcal adhesins.
To identify new components involved in the phenomenon of transformation in Streptococcus pneumoniae, a library of potential mutants has been generated by random insertion of an erythromycin resistance gene. Transformation-deficient mutants were screened using an in situ colony transformation test. The adc locus, which was identified in this search, was cloned and sequenced. Sequence analysis revealed a putative operon of three ORFs (adcC, adcB and adcA) with homology to ATP-binding cassette (ABC) transport operons encoding streptococcal adhesins such as ScaA of S. gordonii and FimA of S. parasanguis. adcA can encode a lipoprotein of 313 amino acid residues containing a putative metal-binding site. The polypeptide shows about 30% sequence identity with ScaA and FimA. We discuss evidence which leads us to propose that AdcA, together with a set of 14 proteins including ScaA, FimA and homologous adhesins, defines a new family of external solute-binding proteins, cluster 9, specific for metals.
Laboratoire de Microbiologie et Génétique moléculaire CNRS-UPR 9007, Université Paul Sabatier, Toulouse, France.
A
A
Dintilhac
J P
JP
Claverys
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D000595:Amino Acid Sequence; D001422:Bacterial Adhesion; D001426:Bacterial Proteins / Q000235:genetics; D003001:Cloning, Molecular; D036781:Fimbriae Proteins; D008074:Lipoproteins / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics; D008670:Metals; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009154:Mutation; D016366:Open Reading Frames / Q000235:genetics; D009876:Operon / Q000235:genetics; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013296:Streptococcus pneumoniae / Q000235:genetics*; D014169:Transformation, Bacterial / Q000235:genetics*
D018528:ATP-Binding Cassette Transporters; C114936:AdcA protein, Streptococcus; C114935:AdcB protein, Streptococcus; C114934:AdcC protein, Streptococcus; D001426:Bacterial Proteins; D008074:Lipoproteins; D008565:Membrane Proteins; D008670:Metals; C079722:ScaA protein, Streptococcus gordonii; C063055:fimbrillin; D036781:Fimbriae Proteins