Microdialysis assessment of 5-fluorouracil release from thermosensitive magnetoliposomes induced by an electromagnetic field in tumor-bearing mice.
The current study was designed to evaluate the properties of thermosensitive magnetoliposomes (TMs), a new drug carrier proposed by the authors, in an electromagnetic field pertaining to their selective heating and drug release under an in vivo condition. TMs containing 5-fluorouracil (5-FU) were prepared by reverse-phase evaporation, injected into the tumor mass of B 16-BL6 melanoma in mice, and selectively heated by a 500-kHz electromagnetic field. The release profile of 5-FU from TMs was examined by using a microdialysis technique. The temperature of TMs in the tumor was effectively elevated to 42 degrees C and maintained at this temperature, overcoming the "cooling effect" of blood flow and surrounding tissues. The release kinetics of 5-FU from TMs was successfully analyzed by physiological modeling, which allows the prediction of intratumor drug concentrations during electromagnetic field exposure under various conditions. In conclusion, this study first demonstrated an in vivo evidence for the electromagnetic field-induced thermosensitive release of a drug from TMs in a tumor with the use of microdialysis.
Department of Hospital Pharmacy, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
E
E
Viroonchatapan
H
H
Sato
M
M
Ueno
I
I
Adachi
J
J
Murata
I
I
Saiki
K
K
Tazawa
I
I
Horikoshi
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000378:metabolism*; D004337:Drug Carriers; D004574:Electromagnetic Fields; D005260:Female; D005472:Fluorouracil / Q000097:blood / Q000378:metabolism*; D008081:Liposomes; D008546:Melanoma, Experimental / Q000097:blood / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D017551:Microdialysis / Q000379:methods; D008954:Models, Biological; D013821:Thermometers; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D004337:Drug Carriers; D008081:Liposomes; D005472:Fluorouracil