Intestinal amoebiasis: antibody-secreting cells and humoral antibodies.
Splenic plasma cell response and systemic antibody response to intestinal amoebiasis were studied in C3H/HeJ mice from 5 to 60 days post-inoculation with Entamoeba histolytica. At various time intervals specific antibody-secreting cells (ASC) in the spleen were measured in infected mice and non-infected control mice by enzyme-linked immunospot (ELISPOT) assay. Serum antibodies were measured by enzyme-linked immunosorbent assay (ELISA). The infected animals showed high IgA ASC from 30 to 50 days post-inoculation as compared to IgM and IgG ASC. However, class-specific serum antibody showed high IgG titre from 30 to 60 days post-inoculation as compared to IgM and IgA serum titres. Our results suggest that E. histolytica trophozoites can induce a plasma cell response in the spleen that is different from anti-amoebic antibody response in serum.
Department of Immunology, Universidad Nacional Autonoma de Mexico, D.F.
P K
PK
Ghosh
S
S
Gupta
L R
LR
Leon
R
R
Ghosh
B H
BH
Ruiz Ordaz
L
L
Ortiz-Ortiz
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology*; D004404:Dysentery, Amebic / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H
D000913:Antibodies, Protozoan