Nucleotide sequences of the trailer, nucleocapsid protein gene and intergenic regions of Newcastle disease virus strain Beaudette C and completion of the entire genome sequence.
The nucleotide sequences of the nucleocapsid protein (NP) gene, the intergenic regions in the nucleocapsid protein (NP)-phosphoprotein (P), P-matrix protein (M) and M-fusion glycoprotein gene junctions and the trailer region of a virulent Newcastle disease virus (NDV) strain Beaudette C were determined. The NP gene is 1747 nt long and encodes a protein of 489 amino acids. Each of the intergenic sequences determined is 1 nt long and, including the previously published intergenic sequences, the gene junction sequences varied in length from 1-47 nt and lacked any sequence identity. The 5' trailer region is 113 nt in length. Comparison of the sequences of the terminal leader and trailer regions of Beaudette C strain with those of nonvirulent strain B1 showed a high level of conservation, indicating the likelihood of these elements not being a factor in virulence. Together with previously published data, this report completes the sequence of the 15,186 nt genomic RNA of NDV strain Beaudette C.
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park 20742, USA.
S
S
Krishnamurthy
S K
SK
Samal
D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000235:genetics*; D019251:Nucleocapsid / Q000235:genetics*; D012367:RNA, Viral / Q000737:chemistry*
D012367:RNA, Viral