Interferon yield and MHC antigen expression of human medulloblastoma cells and its suppression during dibutyryl cyclic AMP-induced differentiation: do medulloblastoma cells derive from bipotent neuronal and glial progenitors?
1. Human medulloblastoma (ONS-76), a central nervous system (CNS)-derived undifferentiated cell line, was found to possess glial characteristics as defined by responses in the interferon (IFN) system; ONS-76 cells produced as much IFN-beta as human fibroblast and glioma cells by viral infection and poly(I):poly(C) induction. 2. Major histocompatibility complex (MHC) class I antigens were also induced under IFN-beta stimulation. ONS-76 cells expressed neurofilament protein, as shown by Northern blot analysis, and morphological differentiation was induced by dibutyryl cyclic AMP (dcAMP). 3. Expression of IFN-beta and MHC class I antigens was suppressed in ONS-76 cells during the dcAMP-induced differentiation. 4. These results showed that ONS-76 cells possessed a glial property in IFN system responses and a neuronal property in cytoskeleton protein, suggesting that the precursors of medulloblastoma may be characterized as bipotent neuronal and glial progenitors in CNS.
Institute for Molecular and Cellular Biology, Osaka University, Japan.
K C
KC
Park
K
K
Shimizu
T
T
Hayakawa
D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D048429:Cell Size; D002528:Cerebellar Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D008527:Medulloblastoma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D009522:Newcastle disease virus / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D015395:Histocompatibility Antigens Class I; D007369:Interferon Inducers; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D003994:Bucladesine; D016899:Interferon-beta; D011070:Poly I-C