Detection of Toxoplasma gondii in 94 placentae from infected women by polymerase chain reaction, in vivo, and in vitro cultures.
The biological diagnosis of congenital toxoplasmosis at birth is important to determine the infant's treatment. The aim of this study was to evaluate the placenta results in the congenital toxoplasmosis diagnosis and to compare them with those obtained with other samples collected at birth (cord blood and newborn blood). A total of 94 placentas, of which 33 came from fetuses suspected of or with proven congenital toxoplasmosis (CT+) and 61 from definitely or probably non-infected fetuses (CT-), was analysed by in vitro culture, mouse inoculation and polymerase chain reaction (PCR). The PCR sensitivity was higher (60.9 per cent) than that of cell culture (29.6 per cent) and mouse inoculation (51.5 per cent) but the number of PCR positive results in CT - patients was also higher (9.5 per cent). The presence of Toxoplasma gondii in the placenta tissues was the only argument at birth (IgM and neosynthesized Ig were negative) in three out of the 33 CT+ cases. The detection of IgM by ELISA and ISAGA and the detection of neosynthesized Ig by immunoblotting were more satisfactory to diagnose congenital toxoplasmosis but the placenta analysis was important to improve the sensitivity of the diagnosis at birth, especially when the prenatal diagnosis was negative or not performed.
Département de Parasitologie-Mycologie, Centre Hospitalier Universitaire, Grenoble, France.
H
H
Fricker-Hidalgo
H
H
Pelloux
C
C
Racinet
I
I
Grefenstette
C
C
Bost-Bru
A
A
Goullier-Fleuret
P
P
Ambroise-Thomas
D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D018929:Cell Culture Techniques; D016054:DNA, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005312:Fetal Blood / Q000469:parasitology; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D010920:Placenta / Q000737:chemistry / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D011296:Prenatal Diagnosis / Q000379:methods; D012680:Sensitivity and Specificity; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology; D014125:Toxoplasmosis, Congenital / Q000097:blood / Q000175:diagnosis*
D000913:Antibodies, Protozoan; D016054:DNA, Protozoan