Effects of inhibitors of nitric oxide synthase on isolated uteri of fasting rats.
The effects of inhibitors of nitric oxide synthase on the glucose metabolism of uteri isolated from 4-day underfed rats were studied. In control rats receiving normal feeding, the addition of indomethacin (5 x 10(-6) M); acetyl salicylic acid (10(-4) M); 400 microM of N(G)methyl-L-arginine, (L-NMMA) or 400 microM of sodium nitroprusside (SNP), does not modify the production of 14CO2 from U14C-glucose. On the contrary, in fasted rat uteri, indomethacin increases glucose oxidation significantly, while acetyl salicylic acid does not alter it. Also, the addition of L-NMMA has no effect. In another group of experiments, in the preparations containing indomethacin of uteri isolated from underfed rats, the addition of L-NMMA significantly changes the effect of indomethacin. Another inhibitor of nitric oxide synthase, N(omega)nitro-L-arginine methyl ester (L-NAME), or hemoglobin (2 microg ml(-1)) a nitric oxide scavenger have the same effects while N(omega)nitro arginine-D-methyl ester (D-NAME) does not. However (SNP), a nitric oxide donor, does not alter the production of 14CO2 in uteri isolated from fasted rats. These results show that in underfed rats, indomethacin increases glucose oxidation independently from its inhibiting effect on cyclooxygenase. Specific inhibitors of nitric oxide synthase can reverse this effect.
Cátedra de Fisiología, Facultad de Odontología, Universidad Nacional de Córdoba, Ciudad Universitaria, Argentina.
M L
ML
Campos
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Casalino-Matsuda
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JA
Linares
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A
Goldraij
D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D005215:Fasting / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D007213:Indomethacin / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D014599:Uterus / Q000187:drug effects* / Q000378:metabolism; D019323:omega-N-Methylarginine / Q000494:pharmacology
D002250:Carbon Radioisotopes; D002245:Carbon Dioxide; D009599:Nitroprusside; D019323:omega-N-Methylarginine; D019001:Nitric Oxide Synthase; D005947:Glucose; D001241:Aspirin; D007213:Indomethacin