[Design of a curve model for the detection of human immunoglobulin G against herpes simplex virus in ultramicroELISA from a single serum dilution].
The use of a curve model in the application of a ultramicroELISA technique for the detection of human immunoglobulin G against herpes simplex virus is reported. Based on end point titration (linear regression) of 100 initial sera, 51 of these, with r2 > 0.98, were selected, and 4 curve models were elaborated to relate the natural fluorescence logarithm for 4 dilutions with the previously determined natural end point titration logarithm. The curve corresponding to the 1:40 (r2 = 0.9645) was selected to evaluate 39 additional serum samples whose approximate titration was known through the graphic design of the 4 dilutions. An 89% coincidence was found regarding the latter. Divergence of the other 11% was due to the lack of accuracy of the graphic method in the points near the cutline.
Instituto de Medicina Tropical Pedro Kouri.
M A
MA
Angeles Ribas
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A
Otero
M
M
Alvarez
M
M
Marrero
S
S
Vázquez
D000465:Algorithms; D000914:Antibodies, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D012044:Regression Analysis; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G
Diseño de una curva patrón para la detección de inmunoglobulina G humana contra virus herpes simple en ultramicroELISA a partir de una dilución única del suero.