Mu gem2ts DNA integration is not necessary for induction of synchrony of cell division in Escherichia coli K12.
The gem2ts mutant of bacteriophage Mu induced synchrony of cell division on bacteria surviving infection. Induction of synchronous growth could also be observed as a response to the entire infected bacterial population, as in the case of infection of hic mutants, a peculiar class of gyrB alleles. After Mu wild-type or Mu gem2ts infection of hic mutants, there was a lack of viral DNA integration and replication, while phage gene expression (including that of A gene, coding for the transposase) seemed to be quite normal. These data indicate that the mechanism of bacterial synchronization induced by Mu gem2ts does not require integration nor replication of the phage DNA.
Bobrowicz
P
P
Dipartimento di Biologia, Università di Roma Tor Vergata, Italy.
Paolozzi
L
L
Ghelardini
P
P
D010583:Bacteriophage mu / Q000235:genetics*; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D004926:Escherichia coli / Q000166:cytology / Q000254:growth & development* / Q000821:virology; D008242:Lysogeny / Q000502:physiology*; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000032:analysis
D004269:DNA, Bacterial; D004279:DNA, Viral; D012329:RNA, Bacterial