Quality control of pentosane polysulfate by capillary zone electrophoresis using indirect detection.
Pentosane polysulfate sodium salt (PPS) is a mixture of multiply charged anionic polysaccharides, used for urological treatment. Several constituents of the polysaccharide can be characterized by a highly reproducible fingerprint. In comparison with earlier approaches the separation efficiency has been further improved using an anionic benzene-1,2,4-tricarboxylic acid buffer (8.7 mmol l-1, pH = 4.9) with indirect UV detection (lambda = 217 nm) and a special capillary pretreatment (1 M NaOH for 10 h at 25 degrees C applying -20 kV). The method has been optimized with regard to buffer concentration and pH. The robustness was tested on several capillaries. PPS was separated from all major synthetic impurities such a sulfate, chloride and acetate. Twelve PPS batches from two manufactures were measured and compared.
Department of Pharmacy and Food Chemistry, University of Würzburg, Germany.
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Degenhardt
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Benend
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Wätzig
D002240:Carbohydrate Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D008969:Molecular Sequence Data; D010426:Pentosan Sulfuric Polyester / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D010426:Pentosan Sulfuric Polyester