Sequence element required for efficient -1 ribosomal frameshifting in red clover necrotic mosaic dianthovirus.
The RNA-1 of the bipartite red clover necrotic mosaic dianthovirus (RCNMV) genome encodes the 88-kDa polymerase. The polymerase is translated from both 5' proximal and internal open reading frames by a -1 ribosomal frameshifting event. A shifty heptanucleotide conforming to the simultaneous slippage model is identified, and a downstream stem-loop structure and atypical pseudoknot are predicted. A beta-glucuronidase reporter assay identified a 118-nucleotide element containing both the shifty heptanucleotide and the predicted secondary structures that were required for efficient -1 ribosomal frameshift expression in vivo. A series of site-directed and compensatory mutations affecting the base-paired regions of the predicted secondary structure were introduced into a RCNMV RNA-1 cDNA clone from which infectious transcripts were derived. Mutations that destroyed the predicted pseudoknot had no effect on frameshifting efficiency in vitro or infectivity of the virus, whereas mutations destabilizing the stem-loop structure abolished both ribosomal frameshifting in vitro and biological activity. These results demonstrate the essential role of a predicted secondary structure that does not involve a pseudoknot in the expression of the RCNMV polymerase by ribosomal frameshifting.
Kim
K H
KH
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, 27695-7616, USA.
Lommel
S A
SA
D000595:Amino Acid Sequence; D020029:Base Pairing; D001483:Base Sequence; D002478:Cells, Cultured; D007887:Fabaceae / Q000821:virology; D018965:Frameshifting, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D016297:Mutagenesis, Site-Directed; D009690:Nucleic Acid Conformation; D010946:Plants, Medicinal; D010947:Plants, Toxic; D011523:Protoplasts / Q000821:virology; D012333:RNA, Messenger / Q000737:chemistry; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins; D014026:Nicotiana / Q000821:virology
D012333:RNA, Messenger; D012367:RNA, Viral; D011993:Recombinant Fusion Proteins