Morphologic and cytochemical characteristics of blood cells from Hawaiian green turtles.
OBJECTIVE
To identify and characterize blood cells from free-ranging Hawaiian green turtles, Chelonia mydas.


METHODS
26 green turtles from Puako on the island of Hawaii and Kaneohe Bay on the island of Oahu.


METHODS
Blood was examined, using light and electron microscopy and cytochemical stains that included benzidine peroxidase, chloroacetate esterase, alpha naphthyl butyrate esterase, acid phosphatase, Sudan black B, periodic acid-Schiff, and toluidine blue.


RESULTS
6 types of WBC were identified: lymphocytes, monocytes, thrombocytes, heterophils, basophils, and eosinophils (small and large). Morphologic characteristics of mononuclear cells and most granulocytes were similar to those of cells from other reptiles except that green turtles have both large and small eosinophils.


CONCLUSIONS
Our classification of green turtle blood cells clarifies improper nomenclature reported previously and provides a reference for future hematologic studies in this species.
US Geological Survey, Biological Resource Division, National Wildlife Health Center, Honolulu, HI 96850, USA.
T M
TM
Work
R E
RE
Raskin
G H
GH
Balazs
S D
SD
Whittaker
D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001773:Blood Cells / Q000166:cytology* / Q000648:ultrastructure; D001800:Blood Specimen Collection / Q000662:veterinary*; D006254:Hawaii; D007124:Immunoenzyme Techniques / Q000662:veterinary; D008854:Microscopy, Electron / Q000662:veterinary; D012016:Reference Values; D014426:Turtles / Q000097:blood*