Fibrinolytic activity of earthworms extract (G-90) on lysis of fibrin clots originated from the venous blood of patients with malignant tumors.
u-PA is secreted by the most malignant tumors. As a response to u-PA synthesis surrounding cells synthetize inhibitors of plasminogen activators for tissue protection. Plasminogen activators were found also in earthworm tissue. From the tissue homogenate of earthworm Eisenia foetida the glycolipoprotein mixture named G-90 was isolated. It contains two serine proteases (P I, P II) with fibrinolytic and anticoagulative activities. The fibrinolytic activity of G-90, P I and P II was tested in an in vitro euglobulinic test applied to fibrin clot from blood plasma of patients suffered from malignant tumors. G-90 and above-mentioned proteases applied in this study showed euglobulinic time proportionally with the concentrations of added substances. The influence of G-90 on the fibrinolysis rate does not depend only on its concentration, but depends too on histological type of tissue (organ) where the malignant tumors are located. Enzyme P I and P II do not show this activity.
Faculty of Veterinary Medicine, Department of Biology, Zagreb, Croatia. mthrzenj@rudjer.irb.hr
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Hrzenjak
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Popović
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Tiska-Rudman
D000328:Adult; D000368:Aged; D000818:Animals; D001780:Blood Coagulation Tests; D005260:Female; D005342:Fibrinolysis / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000502:physiology; D009369:Neoplasms / Q000097:blood*; D009835:Oligochaeta / Q000737:chemistry*; D009928:Organ Specificity; D012697:Serine Endopeptidases / Q000302:isolation & purification / Q000494:pharmacology*; D014020:Tissue Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D014568:Urokinase-Type Plasminogen Activator / Q000502:physiology; D014680:Veins
D005343:Fibrinolytic Agents; D009363:Neoplasm Proteins; D014020:Tissue Extracts; D012697:Serine Endopeptidases; D014568:Urokinase-Type Plasminogen Activator