Consistent repeated M- and H-Wave recording in the hind limb of rats.
Sensory and motor conduction velocities calculated from latencies of H reflexes and M waves in rat hind limbs have been used to assess experimental peripheral neuropathy. Amplitudes and latencies vary with recording location, and are seldom assessed directly. Using subcutaneous electrodes on the foot, we recorded consistent M waves and H reflexes while stimulating the sciatic or tibial nerve. The late wave disappeared when dorsal roots were cut, verifying that it was an H reflex. However, stimulus-response characteristics differed from those in humans: (a) the threshold was often higher than for M waves; (b) stimulus intensity eliciting a maximum H-reflex amplitude (Hmax) was often higher than adequate for a maximum M-wave amplitude; and (c) the amplitudes of H reflexes stimulated with intensities supramaximal for the M wave were over 90% of Hmax. H reflexes and M waves recorded repeatedly in rats can be useful in assessing sensory and motor function in models of neuropathy, using amplitudes as well as conduction velocities.
Cliffer
K D
KD
Regeneron Pharmaceuticals, Inc., Tarrytown, New York 10591, USA.
Tonra
J R
JR
Carson
S R
SR
Radley
H E
HE
Cavnor
C
C
Lindsay
R M
RM
Bodine
S C
SC
DiStefano
P S
PS
D000818:Animals; D004195:Disease Models, Animal; D004558:Electric Stimulation; D004594:Electrophysiology; D005260:Female; D006181:H-Reflex / Q000502:physiology*; D006614:Hindlimb / Q000294:innervation / Q000502:physiology*; D006801:Humans; D009046:Motor Neurons / Q000502:physiology; D009431:Neural Conduction / Q000502:physiology; D009475:Neurons, Afferent / Q000502:physiology; D010523:Peripheral Nervous System Diseases / Q000175:diagnosis / Q000503:physiopathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D011930:Reaction Time / Q000502:physiology; D019051:Rhizotomy; D012684:Sensory Thresholds / Q000502:physiology; D013126:Spinal Nerve Roots / Q000601:surgery