Comparison of the intergenic spacers and 3' end regions of the large subunit (28S) ribosomal RNA gene from three species of Schistosoma.
The sequences for the entire intergenic spacer (IGS) and 3' end region of the large subunit (28S) ribosomal RNA gene are presented for Schistosoma intercalatum, S. haematobium and S. mansoni. The IGS was found to have at least 2 size formats for S. intercalatum and S. haematobium and the region for all 3 species contains numerous repeats and evidence of recombination. An A + T rich sequence found in S. intercalatum and S. haematobium is described and its possible function and origins are discussed. The amplification of this region by means of the polymerase chain reaction can be used to discriminate clearly between the 3 species involved in this study. The putative end of the 28S gene is identified.
Department of Zoology, Natural History Museum, London. rak@nhm.ac.uk
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Rollinson
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry; D018509:DNA, Helminth / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012339:RNA, Ribosomal, 28S / Q000737:chemistry / Q000235:genetics*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D012547:Schistosoma / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D017931:DNA Primers; D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012339:RNA, Ribosomal, 28S