A new, fast, and very sensitive bioluminescence assay for phospholipases A and C.
A new, simple, and very sensitive assay for phospholipase A and C is described. The assay is based on the bioluminescence developed by the mutant of the bacterium Beneckea harveyi as a response to myristic acid released from dimyristoyl phosphatidylcholine by either phospholipase A or by a phospholipase C-lipase coupled system. It is possible to assay these enzymes at a rate corresponding to a release of as little as 1 to 2 pmol of myristic acid per minute.
Ulitzur
S
S
Department of Food Engineering and Biotechnology, Technion, Haifa, Israel.
Heller
M
M
D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements; D009154:Mutation; D010713:Phosphatidylcholines; D010741:Phospholipases A / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D012680:Sensitivity and Specificity; D010738:Type C Phospholipases / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D014733:Vibrio / Q000201:enzymology / Q000235:genetics
D010713:Phosphatidylcholines; D010741:Phospholipases A; D010738:Type C Phospholipases