Two iron-regulated putative ferric siderophore receptor genes in Bordetella bronchiseptica and Bordetella pertussis.
Two iron-regulated genes with deduced homology to TonB-dependent ferric siderophore receptors were cloned from Bordetella bronchiseptica by screening a library of TnphoA insertion mutants. bfrB and bfrC were iron-repressed in B. bronchiseptica by a Fur-dependent mechanism, and were expressed from promoters overlapped by potential Fur-binding sites. Both genes were highly conserved among Bordetella species and were also iron-regulated in Bordetella pertussis. bfrB and bfrC mutants of both species and a bfrB-bfrC double mutant of B. bronchiseptica had no discernible defects in utilization of known iron sources for Bordetella.
Centers for Disease Control and Prevention, Respiratory Diseases Laboratory Section, Atlanta, GA 30333, USA.
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D000469:Alkaline Phosphatase / Q000032:analysis; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D016950:Bordetella bronchiseptica / Q000235:genetics* / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004758:Enterobactin / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D006877:Hydroxamic Acids; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000378:metabolism* / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; C115331:BfrB protein, Bordetella; C115332:BfrC protein, Bordetella; D004269:DNA, Bacterial; D005290:Ferric Compounds; D006877:Hydroxamic Acids; C034477:RNA primers; D012329:RNA, Bacterial; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D017262:Siderophores; C092056:alcaligin; C035902:siderophore receptors; D004758:Enterobactin; D012313:RNA; D000469:Alkaline Phosphatase