Sulphated glycosaminoglycans prevent the neurotoxicity of a human prion protein fragment.
Although a number of features distinguish the disease isoform of the prion protein (PrPSc) from its normal cellular counterpart (PrPC) in the transmissible spongiform encephalopathies (TSEs), the neuropathogenesis of these diseases remains an enigma. The amyloid fibrils formed by fragments of human PrP have, however, been shown to be directly neurotoxic in vitro. We show here that sulphated polysaccharides (heparin, keratan and chondroitin) inhibit the neurotoxicity of these amyloid fibrils and this appears to be mediated via inhibition of the polymerization of the PrP peptide into fibrils. This provides a rationale for the therapeutic effects of sulphated polysaccharides and suggests a rapid in vitro functional screen for TSE therapeutics.
Centro de Biolog approximately ía Molecular, Universidad Autónoma de Madrid, 28049-Madrid, Spain.
M
M
Pérez
F
F
Wandosell
C
C
Colaço
J
J
Avila
D002807:Chondroitin / Q000378:metabolism / Q000494:pharmacology; D006025:Glycosaminoglycans / Q000378:metabolism / Q000494:pharmacology*; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007632:Keratan Sulfate / Q000378:metabolism / Q000494:pharmacology; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000378:metabolism; D011328:Prions / Q000378:metabolism* / Q000633:toxicity* / Q000648:ultrastructure
D006025:Glycosaminoglycans; D010446:Peptide Fragments; D011328:Prions; D006493:Heparin; D002807:Chondroitin; D007632:Keratan Sulfate