Atypical microtubule organization in undifferentiated human colon cancer cells.
We previously reported that undifferentiated colonic cancer HT-29 cells, unlike the differentiated ones, exhibit unusual organelle distributions and atypical vesicle trafficking patterns, which are microtubule-independent and microfilament-dependent. In the present study, we have analyzed the microtubule network in both phenotypes, using confocal microscopy, and determined the expression levels of some microtubule-associated proteins by quantitative immunoblotting. Differentiated cells exhibited the microtubular organization of polarized epithelial cells. Non-polarized undifferentiated cells presented an atypical microtubule organization as microtubules were localized mainly at the cell 'top'. Immunoblot analysis indicated the absence or low content of several structural and motor microtubule-associated proteins in undifferentiated cells, compared to differentiated cells. This may explain in part their atypical microtubular organization. This study agrees with a crucial role for microfilaments in the intracellular organization of undifferentiated HT-29 cancer cells, while differentiated HT-29 cells exhibit intracellular organization similar to that of normal enterocytic cells, although they are also tumoral.
Germen, faculté de médecine, université Paris-XII, 8, Créteil, France. chazaud@univ-paris 12.fr
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D002454:Cell Differentiation / Q000502:physiology; D003110:Colonic Neoplasms / Q000737:chemistry / Q000473:pathology* / Q000648:ultrastructure; D019073:HT29 Cells; D006801:Humans; D018613:Microscopy, Confocal; D008869:Microtubule-Associated Proteins / Q000032:analysis; D008870:Microtubules / Q000648:ultrastructure*; D009363:Neoplasm Proteins / Q000032:analysis; D010641:Phenotype
D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins