Nocistatin reverses nociceptin inhibition of glutamate release from rat brain slices.
We have examined the effects of the recently described heptadecapeptide nocistatin on K+-evoked glutamate release from rat cerebrocortical slices in vitro. In vivo, nocistatin reverses the action of nociceptin. Nocistatin (100 nM, n = 7) did not inhibit K+-evoked glutamate release alone. Nociceptin (100 nM) inhibited glutamate release by 51.7 +/- 8.3% (P < 0.05, n = 6) and this was fully reversed by nocistatin (100 nM). Nocistatin also appears to be an antagonist of nociceptin action in vitro.
University Department of Anaesthesia, Leicester Royal Infirmary, UK.
B
B
Nicol
D G
DG
Lambert
D J
DJ
Rowbotham
E
E
Okuda-Ashitaka
S
S
Ito
D
D
Smart
A T
AT
McKnight
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D018698:Glutamic Acid / Q000378:metabolism*; D018847:Opioid Peptides / Q000037:antagonists & inhibitors* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid / Q000819:agonists; D000097629:Nociceptin
D018698:Glutamic Acid; D018847:Opioid Peptides; D011957:Receptors, Opioid; D000097629:Nociceptin; C111148:nocistatin