Sensitive enzyme immunoassay for anti-beta-lactoglobulin IgG in serum.
A sensitive enzyme immunoassay for anti-beta-lactoglobulin immunoglobulin G (IgG) in serum is described. Serum containing anti-beta-lactoglobulin IgG was reacted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-beta-lactoglobulin conjugate and beta-lactoglobulin-peroxidase conjugate. The complex formed from the three components was trapped onto polystyrene balls coated with anti-2,4-dinitrophenyl group IgG, eluted with epsilon N-2,4-dinitrophenyl-L-lysine and transferred to polystyrene balls coated with anti-human IgG.gamma-chain IgG. Bound peroxidase activity was determined by fluorometry. This enzyme immunoassay was 100- to 1000-fold more sensitive and more reliable than the enzyme-linked immunosorbent assay (ELISA). Anti-beta-lactoglobulin IgG was detected in 91% of healthy subjects using this method.
Division of Maternal and Child Health Science, National Institute of Health and Nutrition, Tokyo, Japan.
K
K
Hirota
S
S
Hashida
E
E
Ishikawa
M
M
Totani
D000906:Antibodies / Q000097:blood / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood*; D007782:Lactoglobulins / Q000276:immunology*; D012680:Sensitivity and Specificity
D000906:Antibodies; D007074:Immunoglobulin G; D007782:Lactoglobulins