Purification of small peptides labeled with Bolton-Hunter reagent.
A method utilizing high-voltage electrophoresis on paper is described whereby a pentapeptide (Asp-Ser-Asp-Pro-Arg) labeled with Bolton-Hunter reagent is separated from hydrolyzed reagent and unreacted peptide and is recovered from the electrophoretogram in high yield. The general applicability to other peptides is discussed.
Coutts
S M
SM
Department of Biochemistry and Drug Disposition, USV Pharmaceutical Corporation, Tuckahoe, New York 10707, USA.
Reid
D M
DM
D004590:Electrophoresis, Paper / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000302:isolation & purification; D007202:Indicators and Reagents; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D013388:Succinimides
D007202:Indicators and Reagents; D010446:Peptide Fragments; D010455:Peptides; D013388:Succinimides; D007073:Immunoglobulin E; C022581:Bolton-Hunter reagent; C040297:pentigide