Perfusing dentine with horse serum or physiologic saline: its effect on adhesion of dentine bonding agents.
Freshly prepared dentine specimens of human teeth were perfused with either horse serum or physiologic saline. After application of AllBond2, ART Bond, Syntac or an experimental dentine bonding agent called P-Bond, a composite cylinder was added and cured at the same time. After 1500 thermal cycles with constant imitation of intrapulpal pressure, the shear bond strengths were measured. Resulting shear bond strength values were analysed with Students t-test or Mann-Whitney Test. The values for AllBond2 were not significantly different. The values for ART Bond (P < 0.05) and for Syntac (P < 0.05) were significantly higher if the dentine was perfused with horse serum. For P-Bond (P < 0.001) the values were significantly higher if the dentine was perfused with physiologic saline. According to these results it does not seem to be appropriate to take a clear decision as to which of the two perfusing media investigated might be more suitable to imitate in vivo conditions.
University of Zurich, Center for Dental and Oral Medicine, Department of Fixed and Removable Prosthodontics and Dental Material Sciences, Switzerland.
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C
Augustin
S J
SJ
Paul
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H
Lüthy
P
P
Schärer
D000818:Animals; D001769:Blood; D001840:Dental Bonding; D003782:Dental Pulp / Q000502:physiology; D003804:Dentin / Q000502:physiology / Q000648:ultrastructure*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D019234:Dentinal Fluid / Q000502:physiology*; D006736:Horses; D006801:Humans; D008298:Maleates / Q000737:chemistry; D008422:Materials Testing / Q000379:methods; D008689:Methacrylates / Q000737:chemistry; D011312:Pressure; D019279:Resin Cements / Q000737:chemistry; D012965:Sodium Chloride; D013816:Thermodynamics
C088247:ART Bond; D017220:Dentin-Bonding Agents; D008298:Maleates; D008689:Methacrylates; D019279:Resin Cements; C078484:Syntac dentine adhesive; C100742:All-Bond 2; D012965:Sodium Chloride