Vitamin E supplementation attenuates myointimal proliferation of the abdominal aorta after balloon injury in diet-induced hypercholesterolemic rabbits.
The effects of vitamin E supplementation in a dose of 450 mg/1000 g chow on the myointimal proliferation of the abdominal aorta after balloon injury were studied in 4 groups of rabbits (24 each). The animals were fed regular diet, regular diet plus vitamin E, 1% cholesterol-enriched diet, and 1% cholesterol-enriched diet plus vitamin E. Each animal underwent a balloon injury of the abdominal aorta and left common iliac artery after 2 weeks of feeding. The animals remained on their respective diets thereafter. In 8 balloon-injured and 8 sham-operated animals of each group, the abdominal aortas were harvested 3 days after the procedure for the analysis of prostacyclin and thromboxane A2 synthesis, thiobarbituric acid reactive substances (TBARS) levels, enzyme activities of glutathione reductase (GR) and glutathione peroxidase (GP) as well as reduced (GSH) and oxidized (GSSG) glutathione levels, 3H-thymidine uptake, cholesterol as well as vitamin E contents. In the other 8 balloon-injured rabbits of each group, the tissue was harvested 3 weeks later for the morphometric study. In dependent of high cholesterol feeding, the vitamin E-treated rabbits had lower aortic production of thromboxane B2, higher 6-keto-PGF1 alpha and higher 6-keto-PGF1 alpha/thromboxane B2 ratios in both procedures. The aortic TBARS levels of the rabbits treated high cholesterol alone were significantly higher than the other three groups in both procedures. Balloon injury had a trend to increase TBARS levels and had significantly higher 3H-thymidine uptake (each p < 0.001) than sham operation in each group. Vitamin E supplement to high cholesterol diet or regular chow reduced aortic TBARS levels (p < 0.005 and 0.01, respectively) and 3H-thymidine uptake (p < 0.05 and 0.01, respectively), as well as attenuated myointimal proliferation of the abdominal aorta and left common iliac artery after balloon injury; but only supplement to high cholesterol diet reached statistical significances (both p < 0.05 compared to rabbits fed high cholesterol alone). These results suggest that vitamin E supplement changes prostanoid metabolism to a favorable pattern and reduces lipid peroxidation of the abdominal aortic wall, thus attenuates myointimal proliferation after balloon injury; these presentations are particularly obvious in diet-induced hypercholesterolemic rabbits.
Department of Internal Medicine (Cardiology), National Taiwan University Medical Center, Taipei. mfchen@.ha.mc.ntu.edu.tw
M F
MF
Chen
H C
HC
Hsu
C S
CS
Liau
Y T
YT
Lee
D015121:6-Ketoprostaglandin F1 alpha / Q000378:metabolism; D000800:Angioplasty, Balloon / Q000009:adverse effects*; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001012:Aorta, Abdominal / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division; D002791:Cholesterol, Dietary / Q000494:pharmacology; D004032:Diet; D006937:Hypercholesterolemia / Q000139:chemically induced; D050356:Lipid Metabolism; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood; D008297:Male; D011817:Rabbits; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D013929:Thromboxane B2 / Q000032:analysis / Q000378:metabolism; D013936:Thymidine / Q000493:pharmacokinetics; D017539:Tunica Intima / Q000166:cytology* / Q000187:drug effects / Q000293:injuries; D014810:Vitamin E / Q000032:analysis / Q000494:pharmacology*
D000975:Antioxidants; D002791:Cholesterol, Dietary; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D013936:Thymidine