Cold preserved nerve allografts: changes in basement membrane, viability, immunogenicity, and regeneration.
Rat sciatic nerve graft segments were harvested and pretreated by either placement in the University of Wisconsin Cold Storage Solution at 5 degrees C and storage from 1 to 26 weeks, or repeatedly freezing (-40 degrees C) and thawing (20 degrees C). Following pretreatment, grafts were transplanted as either syngeneic or allogeneic nerve grafts. Storage and freeze-thawing did not affect the Schwann cell basal lamina or laminin distribution of the peripheral nerve. Graft cell viability decreased with increasing time of storage, with some viable cells detectable even after 3 weeks of storage. Freeze-thawed grafts were not viable. Increasing time of storage led to decreasing immune response and graft rejection, but improved regeneration. Freeze-thawed and 26-week stored allografts were nonimmunogenic and rejection was not seen, but regeneration was delayed compared to autografts. Graft storage may become a useful adjunct to clinical nerve allografting to permit elective scheduling of surgery, provide greater time for preoperative tissue testing, and possibly blunt the immune response.
Division of Orthopaedic Surgery, University of Toronto, Ontario, Canada.
P J
PJ
Evans
S E
SE
Mackinnon
A D
AD
Levi
J A
JA
Wade
D A
DA
Hunter
Y
Y
Nakao
R
R
Midha
D000818:Animals; D001485:Basement Membrane / Q000276:immunology / Q000473:pathology / Q000648:ultrastructure; D002470:Cell Survival / Q000502:physiology; D015925:Cryopreservation; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000096:biosynthesis; D006085:Graft Survival / Q000276:immunology*; D007518:Isoantibodies / Q000032:analysis; D007797:Laminin / Q000032:analysis; D008297:Male; D008854:Microscopy, Electron; D009413:Nerve Fibers, Myelinated / Q000737:chemistry / Q000473:pathology / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D051381:Rats; D011912:Rats, Inbred ACI; D011917:Rats, Inbred Lew; D012583:Schwann Cells / Q000473:pathology / Q000648:ultrastructure; D012584:Sciatic Nerve / Q000166:cytology / Q000637:transplantation*; D014184:Transplantation, Homologous; D014855:Wallerian Degeneration / Q000473:pathology
D007518:Isoantibodies; D007797:Laminin; D009419:Nerve Tissue Proteins; D004247:DNA