Reaggregation and binding of cell wall proteins from Candida albicans to structural polysaccharides.
Urea or hot sodium dodecyl sulphate extracted a significant amount of the same proteins from the matrix of the cell wall of the yeast form and mycelial cells of Candida albicans. Gel filtration analysis of the urea-extracted proteins revealed that they occurred in the form of large complexes which were unaffected by up to 8 M urea. Among them, proteins en route to becoming covalently associated within the wall scaffold were identified by their reaction with specific antibodies. When urea was removed by dialysis, some of these proteins specifically reassociated into large aggregates which bound strongly with ConA, whereas others remained soluble in smaller associated products. The ability of some of these proteins to bind to the insoluble wall polysaccharides was also assessed. No self-assembling proteins were able to bind to glucans and/or chitin. Specificity of the binding to polysaccharides made of beta-bound glucosyl or N-acetylglucosaminyl residues was determined by the competitive effect of several disaccharides. Whereas laminaribiose and diacetylchitobiose were strong inhibitors of protein binding to both glucan and chitin, lactose, maltose and sucrose were ineffective.
Sección de Microbiología, Facultad de Farmacia, Universidad de Valencia, Burjassot, Spain.
C
C
Aguado
J
J
Ruíz-Herrera
M
M
Iranzo
R
R
Sentandreu
S
S
Mormeneo
D000908:Antibodies, Fungal / Q000737:chemistry; D001667:Binding, Competitive; D015153:Blotting, Western; D002176:Candida albicans / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D002473:Cell Wall / Q000737:chemistry / Q000648:ultrastructure; D002686:Chitin / Q000378:metabolism*; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000378:metabolism*; D005936:Glucans / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011485:Protein Binding / Q000502:physiology; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility; D014508:Urea / Q000737:chemistry
D000908:Antibodies, Fungal; D005656:Fungal Proteins; D005936:Glucans; D008565:Membrane Proteins; D003208:Concanavalin A; D002686:Chitin; D012967:Sodium Dodecyl Sulfate; D014508:Urea