Fc-binding molecules specific for human IgG1 and IgG3 are present in Echinococcus granulosus protoscoleces.
In this work we describe the presence of Fc-binding activity on the suckers and tegument of E. granulosus protoscoleces. A fraction (PSA-Fc+) from protoscolex somatic antigens was isolated by affinity chromatography on human Fc-gamma1-Sepharose. Analysis by SDS-PAGE of PSA-Fc+ showed that it contained two major components. Using mouse F(ab')2-human Fc chimaeric monoclonal antibodies we verified that PSA-Fc+ bound mainly to human Fc-gamma1 and Fc-gamma3 isotypes. In addition, one of the components of PSA-Fc+ showed proteolytic activity. Both, Fc-binding and proteolytic activities localized on the protoscolex surface, may play a relevant role in the host-parasite interaction.
Cátedra de Inmunologá, Casilla de Correos 1157, Facultad de Química, Facultad de Ciencias, Montevideo, Uruguay.
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Baz
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Carol
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Marco
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Casabó
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Jones
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Dunne
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Nieto
D000818:Animals; D000909:Antibodies, Helminth / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000378:metabolism*; D004446:Echinococcus / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D006790:Host-Parasite Interactions / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism*; D051379:Mice; D011961:Receptors, Fc / Q000378:metabolism*
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc