[Fluorometric method for determination of nitric oxide and nitric oxide synthetase in rat brain].
A simple, sensitive fluorometric method for the determination of nitric oxide and nitric oxide synthetase in rat brain homogenates has been developed. The method is based on the absorption of a reaction product of N-acetylcysteine and nitric oxide overlapping the absorption of quinine sulfate. The lowest concentration that can be determined is 3 x 10(-8) mol.L-1.
Department of Pharmacology, Chinese Academy of Medical Sciences, Beijing.
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Zhang
D000179:Acrylates / Q000737:chemistry / Q000302:isolation & purification*; D000818:Animals; D001923:Brain Chemistry; D005561:Formates / Q000737:chemistry / Q000302:isolation & purification*; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000032:analysis*; D051381:Rats; D013050:Spectrometry, Fluorescence
C115230:2-2'-dimethoxy-3,3'-dihydro-5,5'-oxygen-6,6'-biphenylformic anhydride; D000179:Acrylates; D005561:Formates; C115229:octadecanyl-3-methoxy-4-hydroxybenzeneacrylate; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase