[Flow cytometric analysis of the effects of 50 Hz magnetic fields on mouse spermatogenesis].
The cellular effects of an extremely-low-frequency (ELF) magnetic field on mouse spermatogenesis were assessed by DNA flow cytometry and serum testosterone. Seven week old male ICR mice were exposed to a 50 Hz magnetic field the strength of which was 1.0 m Tesla. Seven mice per treatment group were exposed for 13, 26, 39 or 52 days. For each experimental point, an equal number of mice per sham-treated group were used as a control and were exposed only to the background field below 1 mu Tesla in the same room as the treatment group. In the control mice, the testis cellular DNA content distribution by flow cytometory was characterized by four quantifiable populations; round spermatids (1C), spermatogonia and other diploid cells (2C), spermatogonial cells synthesizing DNA (S-phase) and primary spermatocytes (4C). In animals exposed for 26 days the number of cells in the 4C and the 4C:2C ratio was significantly lower, and the 1C:4C ratio (meiotic transformation) was significantly higher than the corresponding control groups. In animals exposed for 52 days the cell population in 1C and the 1C:2C ratio (total germ-cell transformation) was significantly higher, and the cell population in 2C was significantly lower than the corresponding control groups. The concentration of serum testosterone in animals exposed for 13 days was significantly higher than in the corresponding control group. These changes suggest that long-term exposure to an ELF magnetic field had a possible effect on the proliferation and differentiation of spermatogonia.
Department of Environmental Health, Tokai University School of Medicine, Kanagawa, Japan.
H
H
Furuya
H
H
Aikawa
T
T
Hagino
T
T
Yoshida
K
K
Sakabe
D000818:Animals; D002454:Cell Differentiation / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D004247:DNA / Q000032:analysis; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005434:Flow Cytometry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D013091:Spermatogenesis / Q000528:radiation effects*; D013093:Spermatogonia / Q000166:cytology; D013737:Testis / Q000378:metabolism
D004247:DNA