Transfer of [3H]estrone-[35S]sulfate across guinea pig fetal membranes.
The possible role of fetal membrane deconjugating activity in the movement of a charged steroid conjugate between fetal and maternal compartments was investigated. The ability of amnion and chorion laeve to transfer [3H]estrone-[35S]sulfate was assessed in both orientations of guinea pig tissue at 45 days and near parturition. While early amnion was impermeable, late tissue transferred approximately 50% (w/w) of the substrate in a bidirectional process that was non-saturable and independent of either deconjugation or ATP. Transfer across early chorion was similar to late amnion. Saturation curves from each tissue were superimposable, as were those of the time course. Transfer across both early and late chorion proceeded in the absence of deconjugation, with no effect of tissue orientation or ATP depletion. However, late chorion exhibited a decrease in estrone-sulfate transfer, as verified by concentration dependency and time course analyses, though transport across the tissue remained non-saturable. The results in amnion were congruous with the presence and absence of tight junctions in the epithelium of early and late tissue, respectively. However, sulfoconjugate transfer across early chorion proceeded in the presence of a paracellular barrier, suggesting specialized regulation of the transport process which extended late into gestation.
Goldhawk
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DE
Department of Biochemistry, University of Western Ontario, London, Canada. goldhawk@julian.uwo.ca
Hobkirk
R
R
D000650:Amnion / Q000502:physiology*; D000818:Animals; D001692:Biological Transport; D002823:Chorion / Q000502:physiology*; D004966:Estrogens, Conjugated (USP) / Q000493:pharmacokinetics; D004970:Estrone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D005865:Gestational Age; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007700:Kinetics; D010539:Permeability; D011247:Pregnancy; D013462:Sulfur Radioisotopes; D014316:Tritium
D004966:Estrogens, Conjugated (USP); D013462:Sulfur Radioisotopes; D014316:Tritium; D004970:Estrone; C017296:estrone sulfate