Attachment and growth of human rotaviruses RV-3 and S12/85 in Caco-2 cells depend on VP4.
Studies with human neonatal rotaviruses RV-3 and S12/85 and their reassortants showed that VP4 is a determinant of rotavirus attachment to and growth in Caco-2 cells. The binding of these viruses to MA104 and Caco-2 cells correlated with their growth ability. Virus sensitivity to trypsin and the VP4 fusion region may be implicated in these processes.
Kirkwood
C D
CD
Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Parkville 3052, Victoria, Australia.
Bishop
R F
RF
Coulson
B S
BS
D000595:Amino Acid Sequence; D018938:Caco-2 Cells; D002213:Capsid / Q000235:genetics / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D005814:Genes, Viral; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D012400:Rotavirus Infections / Q000209:etiology / Q000821:virology; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000494:pharmacology
D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; D014357:Trypsin