The UL112/113 gene products of human cytomegalovirus which colocalize with viral DNA in infected cell nuclei are related to efficient viral DNA replication.
The UL112/113 gene products of human cytomegalovirus (HCMV) were shown by transient complementation ori Lyt-dependent DNA replication assay to be early viral proteins required for efficient viral DNA synthesis. By immunofluorescence analysis followed by fluorescence in situ hybridization, we showed that UL112/113 gene products of HCMV are colocalized with viral DNA prior to and during viral DNA replication in infected cell nuclei. We have used an anti-sense RNA approach for functional analysis of the UL112/113 gene in HCMV. The astrocytoma cell line U373-MG was used for permanent expression of the anti-sense UL112/113 gene. Expression of the anti-sense RNA in this cell line significantly blocked expression of UL112/113 gene products and viral DNA replication, indicating that the UL112/113 gene products are related to efficient viral DNA replication.
Department of Cell Regulation, Medical Research Institute, Tokyo Medical and Dental University, Japan.
T
T
Yamamoto
S
S
Suzuki
K
K
Radsak
K
K
Hirai
D002460:Cell Line / Q000821:virology; D002467:Cell Nucleus / Q000821:virology; D003587:Cytomegalovirus / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology*; D004279:DNA, Viral; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006801:Humans; D016133:Polymerase Chain Reaction; D016372:RNA, Antisense / Q000096:biosynthesis / Q000494:pharmacology; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
D004279:DNA, Viral; D016372:RNA, Antisense; D014764:Viral Proteins