Protein cross-linking mediated by metalloporphyrins.
A biomimetic protein cross-linking reaction is described which employs oxidatively-activated manganese and iron porphyrins as the reactive species. A wide range of proteins cross-link under these conditions, but only if they are intimately associated in solution. The reaction is rapid, efficient, and will be useful for the suprastructural analysis of multiprotein complexes.
Department of Chemistry and Biochemistry, University of Texas, Austin 78712, USA.
L A
LA
Campbell
T
T
Kodadek
K C
KC
Brown
D002614:Chelating Agents / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D006861:Hydrogen Peroxide; D007501:Iron / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008665:Metalloporphyrins / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013464:Sulfuric Acids
D002614:Chelating Agents; D003432:Cross-Linking Reagents; D008665:Metalloporphyrins; D011506:Proteins; D013464:Sulfuric Acids; D008345:Manganese; D006861:Hydrogen Peroxide; D007501:Iron; C048813:potassium peroxymonosulfuric acid