Studies on inhibitory activities of fukiic acid esters on germination, alpha-amylase and carboxypeptidase A.
Fukinolic acid (1) and cimicifugic acid A (2), caffeic acid analogs, as well as rosmarinic acid (3) and caffeic acid (4) showed inhibition on seed germination and seedling growth. The potency of 1 and 2 was comparable with that of 3. Compounds 1 and 2 also showed strong inhibitory activities as well as 3 and 4 on alpha-amylase. The activity of 1 was higher than that of acarbose used as a positive control, and its 50% inhibitory concentration (IC50) was 2.41 x 10(-5) M. Compounds 1 and 2 also showed inhibitory activities strong as 3 and stronger than 4 on carboxypeptidase A. The activities of 1 and 2 were higher than that of 1, 10-phenanthroline used as a positive control.
Osaka University of Pharmaceutical Sciences, Takatsuki, Japan.
G
G
Kusano
M
M
Takahira
M
M
Shibano
A
A
Kusano
Y
Y
Okamoto
H
H
Tsujibo
A
A
Numata
Y
Y
Inamori
D002109:Caffeic Acids / Q000494:pharmacology*; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors*; D043422:Carboxypeptidases A; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004952:Esters / Q000494:pharmacology; D018525:Germination / Q000187:drug effects*; D010648:Phenylacetates / Q000494:pharmacology*; D010666:Phenylpropionates / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D012639:Seeds / Q000187:drug effects* / Q000502:physiology; D013386:Succinates / Q000494:pharmacology*; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D002109:Caffeic Acids; D004791:Enzyme Inhibitors; D004952:Esters; D010648:Phenylacetates; D010666:Phenylpropionates; D010936:Plant Extracts; D013386:Succinates; C116092:cimicifugic acid; C116091:fukiic acid; D000516:alpha-Amylases; D002268:Carboxypeptidases; D043422:Carboxypeptidases A; C116090:fukinolic acid