lacZ transgenic mice to monitor gene expression in embryo and adult.
In transgenic experiments, lacZ can be used as a reporter gene for activity of a given promoter. Its main advantage is the ease of visualization in situ, on sections or in whole mount preparations, and the availability of simple protocols. In the following, we describe our procedure for detecting promoter activity in transgenic mice, including choice of lacZ vectors, generation of the transgenic mice, and analysis of expression. We had recently used this protocol to detect tyrosinase gene promoter activity in embryonic and adult brain.
Schmidt
A
A
ISREC (Swiss Institute for Experimental Cancer Research), Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.
Tief
K
K
Foletti
A
A
Hunziker
A
A
Penna
D
D
Hummler
E
E
Beermann
F
F
D000818:Animals; D004622:Embryo, Mammalian / Q000502:physiology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction