Higher plants possess two structurally different poly(ADP-ribose) polymerases.
One of the immediate reactions of the mammalian cell to many environmental stresses is a massive synthesis of poly(ADP-ribose), catalyzed by poly(ADP-ribose) polymerase (PARP). Most of the biological functions attributed to PARP are inferred from experimentation with mammalian cells. In plants, the biology of PARP may be more complicated and diverse than was previously thought. Two poly(ADP-ribose) polymerase homologues were found in plants, the classical Zn-finger-containing polymerase (ZAP) and the structurally non-classical PARP proteins (APP and NAP), which lack the characteristic N-terminal Zn-finger domain. By enzymatic and cytological experiments the recombinant APP protein was shown to be located in the nucleus and to possess DNA-dependent poly(ADP-ribose) polymerase activity in yeast. The nuclear localization was further confirmed by the analysis of transgenic tobacco plants that expressed a translational gene fusion between APP and the bacterial beta-glucuronidase. The app promoter was transcriptionally up-regulated in cells pre-determined to die because of deficiency in a DNA ligase I.
Babiychuk
E
E
Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, Belgium.
Cottrill
P B
PB
Storozhenko
S
S
Fuangthong
M
M
Chen
Y
Y
O'Farrell
M K
MK
Van Montagu
M
M
Inzé
D
D
Kushnir
S
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D011088:DNA Ligases / Q000378:metabolism; D004926:Escherichia coli; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry / Q000235:genetics*; D012441:Saccharomyces cerevisiae; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics; D016335:Zinc Fingers
D009687:Nuclear Proteins; D010940:Plant Proteins; D011065:Poly(ADP-ribose) Polymerases; D011088:DNA Ligases