A computer-controlled variable light attenuator for protection and autoranging of a laser-induced fluorescence detector for capillary zone electrophoresis.
Photodetectors have a limited range over which they can measure light intensities for any particular setting. The intensity of light reaching the detector can be kept within this range by using a liquid crystal variable light attenuator controlled by a computer that continuously checks the amount of light reaching the detector and adjusts the attenuation to an appropriate level. Using such a system we have constructed an intensified charge-coupled device (ICCD) camera-based detector with a dynamic range of over six orders of magnitude which is never exposed to damaging or saturating light levels.
Merz
J M
JM
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA. yoda@biochem.okstate.edu
Mort
A J
AJ
D000818:Animals; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry; D003201:Computers; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D005453:Fluorescence; D006603:Hexuronic Acids; D007834:Lasers; D009281:Naphthalenes; D010783:Photometry; D014466:Ultraviolet Rays
D002241:Carbohydrates; D006603:Hexuronic Acids; D009281:Naphthalenes; C007819:galacturonic acid; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid