Potent suppressive activity of chlorophyll a and b from green tea (Camellia sinensis) against tumor promotion in mouse skin.
Potent antigenotoxic and anti-tumor promoting activities of chlorophyll a from green tea (camellia sinensis) have been shown using in vitro cell culture experiments (Okai Y. et al. (1996) Mutation Res., 370, 11-17). In the present study, the authors analyzed in vivo effects of chlorophyll a and b from green tea on tumor promotion in mouse skin in the following ways. 1. When chlorophyll a and b from green tea were applied before each treatment by a tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on BALB/c mouse skin initiated by 7, 12-dimethylbenz [a] an-thracene (DMBA), they caused significant suppression in a dose-dependent manner against BALB/c mouse skin tumorigenesis. 2. Chlorophyll a and b showed significant suppressive effects against TPA-induced inflammatory reaction such as edema formation in BALB/c mouse ear skin in a dose-dependent fashion. These results suggest that chlorophyll a and b possess potent suppressive activities against tumor promotion in mouse skin.
Department of Human Life Science, Osaka Kun-Ei Women's College, Japan.
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Higashi-Okai
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Okai
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002273:Carcinogens / Q000037:antagonists & inhibitors*; D002734:Chlorophyll / Q000302:isolation & purification / Q000494:pharmacology*; D000077194:Chlorophyll A; D004487:Edema / Q000517:prevention & control; D051379:Mice; D008807:Mice, Inbred BALB C; D010860:Pigments, Biological / Q000302:isolation & purification / Q000494:pharmacology*; D012878:Skin Neoplasms / Q000517:prevention & control*; D013662:Tea / Q000737:chemistry*; D013755:Tetradecanoylphorbol Acetate / Q000037:antagonists & inhibitors*
D002273:Carcinogens; D010860:Pigments, Biological; D013662:Tea; D002734:Chlorophyll; D015127:9,10-Dimethyl-1,2-benzanthracene; C037184:chlorophyll b; D013755:Tetradecanoylphorbol Acetate; D000077194:Chlorophyll A