Antigenic characterization of canine parvovirus strains isolated in Italy.
28 isolates of canine parvovirus type-2 (CPV-2) were obtained from dogs with hemorrhagic gastroenteritis in Italy. The antigenic structure of CPV-2 isolates was characterized, using four discriminating monoclonal antibodies. In addition, four vaccinal strains were examined. Similar to reports from Australia and the United Kingdom, a much higher prevalence of CPV-2a (25/28 isolates) was observed than the other variant type, CPV-2b (3/28 isolates). DNA fragments (2.2 kbp) of representative strains of CPV-2, CPV-2a and CPV-2b were amplified by the polymerase chain reaction (PCR) and the products were digested by the restriction enzymes (RE) RsaI, HpaII, HindIII and PvuII. The RvaI enzyme allows the differentiation of CPV-2 from CPV-2a and CPV-2b.
Dipartimento di Sanita', Patologia, Farmaco-Tossicologia e Benessere Animale, Facolta' di Medicina Veterinaria, Valenzano, Bari, Italy.
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Sagazio
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Tempesta
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Buonavoglia
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F
Cirone
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Buonavoglia
D000818:Animals; D000911:Antibodies, Monoclonal; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000276:immunology*; D002213:Capsid / Q000235:genetics; D004285:Dogs; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D007558:Italy; D010322:Parvoviridae Infections / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000276:immunology* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D014765:Viral Vaccines
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D014765:Viral Vaccines