Enhanced penicillin production by oligosaccharides from batch cultures of Penicillium chrysogenum in stirred-tank reactors.
Alginate and galactomannan-derived oligosaccharides enhanced the production of penicillin G when added to stirred tank reactor cultures of Penicillium chrysogenum. The addition of oligomannuronate and oligoguluronate blocks increased penicillin G yield by 47% and 49%, respectively. The effect of mannan oligosaccharides was found to be more pronounced with 69% higher yield than the control cultures. The maximum increase in the average specific productivity of the oligosaccharide augmented cultures was 55% after addition of mannan oligosaccharides. In addition, a difference was observed in all cases in the accumulation pattern of the intermediate of penicillin biosynthesis, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine.
Biotechnology Department, University of Westminster, London, UK.
B
B
Ariyo
C
C
Tamerler
C
C
Bucke
T
T
Keshavarz
D000464:Alginates / Q000494:pharmacology; D019149:Bioreactors; D005285:Fermentation; D005690:Galactose / Q000031:analogs & derivatives; D007700:Kinetics; D008351:Mannans / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism; D009844:Oligosaccharides / Q000494:pharmacology; D010400:Penicillin G / Q000378:metabolism*; D010406:Penicillins / Q000096:biosynthesis*; D010408:Penicillium chrysogenum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D000464:Alginates; D008351:Mannans; D009842:Oligopeptides; D009844:Oligosaccharides; D010406:Penicillins; C012990:galactomannan; C011638:5-(2-aminoadipyl)cysteinylvaline; D010400:Penicillin G; D005690:Galactose