Up-regulation of the expression of major histocompatibility complex class I antigens by plasmid DNA transfection in non-hematopoietic cells.
The effect of DNA on the surface expression of major histocompatibility (MHC) class I antigens was examined in non-hematopoietic tumor cell lines. Transfection with plasmid DNA via liposome or electroporation significantly increased the surface expression of MHC class I molecules in a transient manner. Northern blot analysis showed that levels of MHC class I mRNA were increased by DNA transfection, probably via transcriptional activation. In contrast, the expression of the MHC class II and beta-actin genes was not affected, suggesting that the up-regulation of MHC class I expression by plasmid DNA works in a gene-specific manner.
Park
J H
JH
Department of Biology, Changwon National University, Kyungnam, South Korea. parkjh@sarim.changwon.ac.kr
Chang
S H
SH
Kim
M C
MC
Shin
S H
SH
Youn
H J
HJ
Kim
J K
JK
Jang
Y S
YS
Kim
C W
CW
D000199:Actins / Q000235:genetics; D000818:Animals; D015152:Blotting, Northern; D004269:DNA, Bacterial / Q000378:metabolism* / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003850:Deoxyribonuclease I / Q000378:metabolism / Q000494:pharmacology; D018274:Electroporation; D005354:Fibrosarcoma / Q000235:genetics / Q000276:immunology / Q000473:pathology; D005786:Gene Expression Regulation; D005802:Genes, MHC Class II; D006410:Hematopoiesis; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism*; D008081:Liposomes; D051379:Mice; D012333:RNA, Messenger; D015533:Transcriptional Activation; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects
D000199:Actins; D004269:DNA, Bacterial; D015395:Histocompatibility Antigens Class I; D008081:Liposomes; D012333:RNA, Messenger; D003609:Dactinomycin; D003850:Deoxyribonuclease I