Poly-L-lysine amplification of protamine immobilization and heparin adsorption.
We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method to augment protamine loading on the fiber, as well as to create multiple layers of immobilized protamine for heparin interaction. Results show that such a method yielded about a threefold increase in protamine loading and, consequently, about a fourfold enhancement in heparin adsorption when compared with the control without poly-L-lysine amplification. This technological improvement may facilitate development of a new generation of protamine filters with capacity and efficacy suitable for various clinical applications in extracorporeal heparin removal.
Zhang
Y
Y
College of Pharmacy, University of Michigan, Ann Arbor 48109-1065, USA.
Singh
V K
VK
Yang
V C
VC
D001672:Biocompatible Materials; D005374:Filtration / Q000295:instrumentation*; D006493:Heparin / Q000737:chemistry; D006494:Heparin Antagonists / Q000737:chemistry; D006801:Humans; D008567:Membranes, Artificial; D010956:Plasmapheresis / Q000295:instrumentation*; D011107:Polylysine; D011479:Protamines / Q000737:chemistry
D001672:Biocompatible Materials; D006494:Heparin Antagonists; D008567:Membranes, Artificial; D011479:Protamines; D011107:Polylysine; D006493:Heparin