Screening male intracytoplasmic sperm injection candidates for mutations of the follicle stimulating hormone receptor gene.
Follicle stimulating hormone (FSH) is considered to be essential for spermatogenesis. Therefore, genetic abnormalities of FSH signalling on testicular Sertoli cells would be expected to affect sperm production negatively in males. Inactivating FSH receptor mutations have been reported earlier in both males and females. All affected males had elevated FSH serum concentrations and abnormal sperm parameters. We postulated that inactivating FSH receptor mutations might be a cause of oligozoospermia or azoospermia and reviewed the clinical data of 151 male intracytoplasmic sperm injection (ICSI) candidates with special attention to FSH serum concentrations. The exclusion criteria for mutation screening of the FSH receptor gene were: a history of operative sterilization or testicular malignancy, congenital abnormality other than cryptorchidism, and a chromosomal aberration or a Y-chromosome microdeletion. The inclusion criteria were: male (ICSI candidate) with azoospermia or oligoasthenoteratozoospermia (OAT) and elevated FSH serum concentrations. In total, 23 males with OAT and five males with azoospermia were tested for mutations of the coding sequences and the intron-exon boundaries of the FSH receptor gene by polymerase chain reaction (PCR) followed by single strand conformation polymorphism analysis (SSCP). Neutral polymorphisms were readily detected using this technique in both probands and controls. None of the 28 selected patients showed a pathogenic FSH receptor mutation. Mutations in the FSH receptor gene are not a common cause of infertility in ICSI candidates.
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
J H
JH
Tuerlings
M J
MJ
Ligtenberg
J A
JA
Kremer
M
M
Siers
E J
EJ
Meuleman
D D
DD
Braat
L H
LH
Hoefsloot
H M
HM
Merkus
H G
HG
Brunner
D003593:Cytoplasm; D005307:Fertilization in Vitro / Q000379:methods; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007248:Infertility, Male / Q000235:genetics* / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008845:Microinjections; D009154:Mutation; D009845:Oligospermia / Q000235:genetics / Q000378:metabolism / Q000628:therapy; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011962:Receptors, FSH / Q000235:genetics*; D013094:Spermatozoa
D011962:Receptors, FSH; D005640:Follicle Stimulating Hormone