Direct human papillomavirus (HPV) sequencing method yields a novel HPV in a human immunodeficiency virus-positive Quebec woman and distinguishes a new HPV clade.
Papillomaviruses of supergroup A exhibit genital tropism and are best known as etiologic agents for benign and malignant cervical lesions in women. A polymerase chain reaction direct sequencing approach with P-33-labeled dideoxynucleotides was used to detect and type human papillomaviruses (HPVs) in cervical biopsies. A novel sequence was found in condylomatous specimens from a human immunodeficiency virus-positive French Canadian woman. The viral gene L1 was sequenced completely, yielding a novel HPV type of supergroup A, named JC9710. This is related to a previously described HPV type from New Mexico, CP8061, and to Colobus monkey papillomavirus 1. Sequence similarity searches and phylogenetic analyses with different software packages clustered the three viral types as a new clade, for which the next available number, A15, was proposed.
Feoli-Fonseca
J C
JC
Department of Pathology, Research Center, University of Montreal, Canada.
Oligny
L L
LL
Filion
M
M
Brochu
P
P
Russo
P A
PA
Yotov
W V
WV
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D001706:Biopsy; D002584:Cervix Uteri / Q000473:pathology; D003218:Condylomata Acuminata / Q000150:complications / Q000235:genetics / Q000473:pathology / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry; D005260:Female; D005838:Genotype; D006679:HIV Seropositivity / Q000150:complications / Q000235:genetics / Q000473:pathology / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011792:Quebec; D016415:Sequence Alignment; D014591:Uterine Diseases / Q000150:complications / Q000235:genetics / Q000473:pathology / Q000821:virology
D004279:DNA, Viral