Mouse cytochrome b561: cDNA cloning and expression in rat brain, mouse embryos, and human glioma cell lines.
A cDNA encoding cytochrome b561 has been isolated from a mouse brain cDNA library, which predicts a protein of 250 amino acids with a deduced Mr of 27,770. Northern blot analysis of different mouse and rat tissues revealed one major mRNA of 3300 bp, which is abundantly distributed in a number of neuroendocrine tissues. In addition, cytochrome mRNA levels in rat brain sections showed the highest distribution of cytochrome b561 in the hypothalamus, hippocampus, thalamus, and striatum, with a moderate level in the cerebral cortex, and the lowest levels in the olfactory bulb and cerebellum. Because non-neuronal cells in the central nervous system contained peptidyl alpha-amidating monooxygenase (PAM), to which cytochrome b561 donates its electrons, we used RT-PCR to document the coexpression of cytochrome b561 with PAM and dopamine beta-hydroxylase (DBH) in glioblastoma cells. Cytochrome b561 expression was detectable in the 11-day-old mouse embryo, and the level of its mRNA increased tenfold by 15 and 17 days of gestation.
Srivastava
M
M
Department of Anatomy and Cell Biology, Uniformed Services University of Health Sciences, Bethesda, MD 20814, USA.
Pollard
H B
HB
Fleming
P J
PJ
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001923:Brain Chemistry; D003001:Cloning, Molecular; D003573:Cytochrome b Group / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics*; D004299:Dopamine beta-Hydroxylase / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005909:Glioblastoma; D005910:Glioma / Q000737:chemistry*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D006899:Mixed Function Oxygenases / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured
D003573:Cytochrome b Group; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D012333:RNA, Messenger; C023646:cytochrome b561; D006899:Mixed Function Oxygenases; D004299:Dopamine beta-Hydroxylase; C035189:peptidylglycine monooxygenase