Clinical effects of the recombinant feline interferon-omega on experimental parvovirus infection in beagle dogs.
The clinical effects of recombinant feline interferon-omega (rFeIFN-omega), produced in silkworm by recombinant baculovirus, were examined in 3-4 month-old beagle dogs given an experimental canine parvovirus type-2 (CPV-2) infection. Clinical symptoms, such as pyrexia, vomiting, anorexia and diarrhea, were observed on day 4 after oral inoculation of 10(7) TCID50 of CPV-2 (cc 238 strain) in almost all the inoculated dogs. From day 4, rFeIFN-omega (1 mega units/kg/day) or physiological saline was administered intravenously to infected dogs for 3 consecutive days. Seven out of 17 dogs treated with physiological saline showed hemorrhagic diarrhea and continuously expressed severe clinical enteritis; one dog died with a large amount of hemorrhagic rice-water stool on day 6 after viral exposure. In contrast, 4 out of 12 dogs treated with rFeIFN-omega showed severe clinical enteritis associated with intermittent diarrhea. Scoring of fecal condition revealed that treatment with rFeIFN-omega significantly shifted the enteritis from a severe to mild form. Furthermore, rFeIFN-omega administered in the morning decreased the number of dogs expressing clinical enteritis in the evening suggesting a rapid effect. Vomiting and anorexia were also improved by treatment with rFeIFN-omega. These results suggest that rFeIFN-omega can reduce severe enteritis caused by CPV-2 infection in dogs.
Department of Microbiology, School of Medicine, Hokkaido University, Sapporo, Japan.
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K
Ishiwata
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Minagawa
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T
Kajimoto
D000818:Animals; D002415:Cats; D004283:Dog Diseases / Q000503:physiopathology / Q000628:therapy*; D004285:Dogs; D005243:Feces / Q000821:virology; D007370:Interferon Type I / Q000627:therapeutic use*; D010322:Parvoviridae Infections / Q000503:physiopathology / Q000628:therapy / Q000662:veterinary*; D017993:Parvovirus, Canine / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000627:therapeutic use; D014162:Transfection
D007370:Interferon Type I; D011994:Recombinant Proteins; C063743:interferon omega 1