The use of video-imaging to assess the sub-lethal impact of plant secondary compounds on Schistosoma mansoni miracidia.
The study describes methods developed for using video-imaging technology to record and measure the velocity of Schistosoma mansoni miracidia. The efficacy of the classical bioassay procedure (a qualitative behavioural assay) was compared with that of the new quantitative protocol, for assessing the sub-lethal impact of a larvicidal dichloromethane extract of the seeds of Millettia thonningii on miracidia. The new technique confirmed the efficacy of the classical bioassay for rapid determination of the lethal and sub-lethal impact of larvicides but also provided quantitative information on sub-lethal impacts on miracidial velocity and shape.
Infection and Immunity Research Group, Division of Life Sciences, King's College London, University of London, Campden Hill Road, Kensington, London, W8 7AH, UK. james.r.lyddiard@kcl.ac.uk
J R
JR
Lyddiard
A
A
Bartlett
B
B
Gray
P J
PJ
Whitfield
D000818:Animals; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry*; D007091:Image Processing, Computer-Assisted; D007814:Larva / Q000187:drug effects; D008752:Methylene Chloride / Q000494:pharmacology*; D009068:Movement / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D012550:Schistosoma mansoni / Q000187:drug effects* / Q000502:physiology; D013696:Temperature; D013997:Time Factors
D010936:Plant Extracts; D008752:Methylene Chloride