Decrease in the concentration of vitamin E in blood and tissues caused by di(2-ethylhexyl) phthalate, a commonly used plasticizer in blood storage bags and medical tubing.
OBJECTIVE
Significant amounts of di(2-ethylhexyl) phthlate (DEHP) leach into blood stored in DEHP-plasticized PVC bags. The aim of this study was to find out whether DEHP at these low levels has any effect on the concentration of vitamin E, an antioxidant which affords protection against free radical damage.


METHODS
DEHP was administered in low doses (150-750 microg/100 g body weight) to rats intraperitoneally and the concentration of vitamin E in the liver and testes was measured. Concentration of vitamin E was also measured in blood stored in glass bottles in the presence and absence of DEHP and in blood stored in DEHP-plasticized PVC bags.


RESULTS
A decrease in the concentration of vitamin E was observed in all cases. Administration of vitamin E to rats and incorporation of vitamin E in the additive solution in the case of blood prevented this decrease.


CONCLUSIONS
DEHP even at very low doses caused a decrease in the concentration of vitamin E in liver and tests of rats given this substance. Blood stored in DEHP-plasticized bags also showed a decrease in the concentration of vitamin E.
Department of Biological Sciences, Peninsula Polymers Limited, Trivandrum, India.
V
V
Manojkumar
K G
KG
Padmakumaran Nair
A
A
Santhosh
K V
KV
Deepadevi
P
P
Arun
L R
LR
Lakshmi
P A
PA
Kurup
D000818:Animals; D001793:Blood Preservation / Q000295:instrumentation; D004051:Diethylhexyl Phthalate / Q000032:analysis / Q000097:blood / Q000494:pharmacology*; D004912:Erythrocytes / Q000737:chemistry; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000187:drug effects; D008297:Male; D010968:Plasticizers / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000737:chemistry / Q000187:drug effects; D014810:Vitamin E / Q000032:analysis* / Q000097:blood
D010968:Plasticizers; D014810:Vitamin E; D004051:Diethylhexyl Phthalate