pzl-1 encodes a novel protein phosphatase-Z-like Ser/Thr protein phosphatase in Neurospora crassa.
The gene and cDNA of a novel protein phosphatase were cloned from Neurospora crassa. The pzl-1 gene encompasses three introns and is localized to the left arm of chromosome I between cyt-21 and Fsr-12. It encodes a protein of 58.3 kDa containing a Ser/Pro rich N-terminal segment, and a C-terminal domain that is similar to the catalytic subunit of type 1 protein phosphatases. The first 51 amino acid residues, including a potential N-myristoylation site, as well as the C-terminal domain (about 300 residues) have a high level of sequence identity with yeast PPZ phosphatases. However, residues 52-208 do not share high similarity with other proteins. The mRNA of pzl-1 was detected in all phases of asexual development of the filamentous fungus.
Department of Medical Chemistry, University Medical School of Debrecen, H-4012 Debrecen, Hungary.
B
B
Szöor
Z
Z
Fehér
T
T
Zeke
P
P
Gergely
E
E
Yatzkan
O
O
Yarden
V
V
Dombrádi
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D005800:Genes, Fungal; D008969:Molecular Sequence Data; D009491:Neurospora / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D010749:Phosphoprotein Phosphatases / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D012331:RNA, Fungal / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis
D012331:RNA, Fungal; D012333:RNA, Messenger; D010749:Phosphoprotein Phosphatases