Monoclonal antibodies directed against extracellular matrix proteins reduce the adherence of Candida albicans to HEp-2 cells.
The presence of the extracellular matrix (ECM) proteins collagen types I and IV, laminin and fibronectin on the surface of HEp-2 cells was confirmed by flow cytometry using monoclonal antibodies. Monoclonal antibodies directed against these ECM proteins reduced the adherence of C. albicans ATCC 44990 to HEp-2 cells, the greatest reductions being evident in assays which incorporated anti-collagen type IV monoclonal antibody. The ability of sugaramines to inhibit the adherence of C. albicans to a variety of cell types has been demonstrated previously and the most significant reduction in C. albicans-HEp-2 adherence was in assays which incorporated 0.2M galactosamine. The combination of anti-collagen IV monoclonal antibody and galactosamine reduced the adherence of C. albicans to HEp-2 cells by approximately 70% (p < 0.05).
Department of Biology, National University of Ireland, Kildare, Ireland.
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Cotter
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Weedle
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Kavanagh
D000911:Antibodies, Monoclonal; D002176:Candida albicans / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D003094:Collagen / Q000276:immunology / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000276:immunology* / Q000378:metabolism; D005353:Fibronectins / Q000276:immunology / Q000378:metabolism; D005434:Flow Cytometry; D005688:Galactosamine / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000276:immunology / Q000378:metabolism
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D005688:Galactosamine; D003094:Collagen