A method of stimulating an immune response with a hapten immobilized on a nitrocellulose membrane.
Hapten refers to a chemical compound of small molar mass (typically less than 1000 daltons) that can bind with an antibody, but cannot initiate an immune response by itself unless it is conjugated to a protein carrier of larger molar mass. A novel method to prepare a hapten to generate anti-hapten immunity without covalent conjugation to a carrier was developed. Coating both water-soluble and -insoluble haptens onto a nitrocellulose membrane effectively presented haptens to the system and caused the generation of specific anti-hapten B lymphocytes and antibodies by immunization both in vitro and in vivo. This method has a potential to substitute for conventional hapten carrier conjugation to generate anti-hapten immunity.
Cell Biology and Immunobiology Division, Development Center for Biotechnology, Taipei, Taiwan, R.O.C.
J J
JJ
Liu
C C
CC
Wang
Y L
YL
Hwang
S
S
Yue
T H
TH
Chang
D000327:Adsorption; D000818:Animals; D003101:Collodion; D006241:Haptens / Q000276:immunology*; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C
D006241:Haptens; D003101:Collodion