The combined affects of modified atmosphere, temperature, nisin and ALTA 2341 on the growth of Listeria monocytogenes.
A cocktail of seven Listeria monocytogenes isolates of food, human and environmental origin was used to assess the antilisterial activity of the bacteriocins nisin and ALTA 2341 in combination with various atmospheres: air, 100% N2, 40% CO2:60% N2, or 100% CO2. Buffered tryptone soya broth (pH 6.0) was used as the growth medium and incubation was at 4 degrees C (21 days) or 12 degrees C (7 days), or when temperature fluctuated between these values for defined periods. It was observed that atmosphere alone influenced the growth rate of L. monocytogenes, with 100% CO2 exerting the greatest inhibition. A 5 log population increase was observed in all atmospheres after 7 days at 12 degrees C. At 4 degrees C a 4-5 log population increase was observed in air, 100% N2 and 40% CO2:60% N2 within 21 days. Growth was prevented by 100% CO2. In the presence of nisin (400 IU/ml), an increase in the lag phase was observed before growth (5 log population increase after 7 days) in all atmospheres at 12 degrees C. This effect was enhanced at 4 degrees C where a maximum 2 log population increase was observed in all atmospheres except 100% CO2, in which growth was prevented. Increasing the concentration of nisin to 1250 IU/ml prevented L. monocytogenes growth in all atmosphere combinations at 4 and 12 degrees C. Two concentrations of ALTA 2341 were also tested. In the presence of 0.1% ALTA 2341 and at 12 degrees C, a 3-5 log population increase was observed in all atmospheres with the exception of 100% CO2, which prevented L. monocytogenes growth. At 4 degrees C, growth was observed in the combination of 0.1% ALTA 2341 and 100% N2 only (3 log population increase). Use of a higher concentration of ALTA 2341 (1.0%) resulted in a population decrease below the detection level within 24 h in all atmosphere/temperature combinations. Re-growth occurred in the presence of 1.0% ALTA 2341 in all atmospheres at 12 degrees C, and in combination with air or 100% N2 at 4 C. When the effectiveness of either nisin or ALTA 2341 and atmosphere was tested against L. monocytogenes as temperature fluctuated for periods between 4 and 12 degrees C, only the combination of 100% CO2 and 1.0% ALTA 2341 prevented growth. Cells surviving exposure to nisin or ALTA 2341 were recovered from 28 of the 32 combinations tested that contained bacteriocin. Nisin survivors remained sensitive to the bacteriocin. ALTA 2341 survivors had become resistant to the bacteriocin.
Food Science Australia, North Ryde Laboratory, Sydney. Lisa.Szabo@foodscience.afisc.csiro.au
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D001430:Bacteriocins / Q000494:pharmacology*; D002245:Carbon Dioxide / Q000494:pharmacology; D003080:Cold Temperature; D015169:Colony Count, Microbial; D005516:Food Microbiology; D005520:Food Preservatives / Q000494:pharmacology*; D006801:Humans; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D015999:Multivariate Analysis; D009561:Nisin / Q000494:pharmacology*; D009584:Nitrogen / Q000494:pharmacology; D012034:Refrigeration
D001430:Bacteriocins; D005520:Food Preservatives; D009561:Nisin; D002245:Carbon Dioxide; D009584:Nitrogen