Protoheme extraction from plant tissue.
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether. After evaporation of the ether, the residue is dissolved in alkaline pyridine, and the protoheme concentration is estimated from a dithionite-reduced-minus-ferricyanide-oxidized spectrum. When compared to some other methods, this procedure gives consistently higher yields.
Department of Biological Sciences, University of Illinois at Chicago Circle 60680, USA.
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LC
Stillman
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Gassman
D000096:Acetone; D004986:Ether; D006418:Heme / Q000302:isolation & purification*; D007202:Indicators and Reagents; D010944:Plants / Q000737:chemistry*; D011725:Pyridines; D015203:Reproducibility of Results; D013053:Spectrophotometry; D013696:Temperature
D007202:Indicators and Reagents; D011725:Pyridines; D004986:Ether; D000096:Acetone; D006418:Heme; C023666:pyridine