Detection of SV40 sequences in human mesothelioma.
The presence of SV40 DNA sequences in British cases of mesothelioma was investigated using previously reported PCR assays. Three different SV40 primer sets were used which gave positive detection rates of 100% (SV.for3/SV.rev), 42% (PYV.for/rev) and 15% (SV.for2/SV.rev). When 26 mesothelioma biopsies were tested. Negative samples included 22 semen samples from AIDS patients, 10 blood donor DNAs and the human mesothelioma cell line H-MESO-1. The problems encountered with these assays and the interpretation of the results are discussed.
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
D J
DJ
Griffiths
A G
AG
Nicholson
R A
RA
Weiss
D001483:Base Sequence; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D008654:Mesothelioma / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D013539:Simian virus 40 / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004279:DNA, Viral