Simultaneous determination of ritonavir and saquinavir, two human immunodeficiency virus protease inhibitors, in human serum by high-performance liquid chromatography.
The aim of this study was to describe an high-performance liquid chromatographic assay for the simultaneous determination of two HIV protease inhibitors, saquinavir and ritonavir, in human serum. The method involved extraction of ritonavir and saquinavir from serum with the aid of solid-phase extraction C18 cartridges followed by high-performance liquid chromatography with a C8 column and ultraviolet detection set at a wavelength of 240 nm. The assay was linear and has been validated over the concentrations range of 0.5-32 microg/ml for ritonavir and 0.075-4.8 microg/ml for saquinavir, from 600 microl serum extracted. In future, the assay will be used to support human population pharmacokinetic studies, and therapeutic drug monitoring for ritonavir and saquinavir.
Clinical Pharmacokinetic Department, Victor Segalen University Bordeaux France and Pharmacy Haut-leveque Hospital, Pessac.
S
S
Frappier
D
D
Breilh
E
E
Diarte
B
B
Ba
D
D
Ducint
J L
JL
Pellegrin
M C
MC
Saux
D019380:Anti-HIV Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017320:HIV Protease Inhibitors / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D019438:Ritonavir / Q000097:blood*; D019258:Saquinavir / Q000097:blood*; D012680:Sensitivity and Specificity
D019380:Anti-HIV Agents; D017320:HIV Protease Inhibitors; D019258:Saquinavir; D019438:Ritonavir