Preparation of lipid-free protein extracts of egg yolk.
1-Butanol extraction of chicken egg yolk homogenates containing 1 M NaCl yields lipid-free aqueous solutions of egg yolk proteins. These solutions, after dialysis, can be applied to a variety of chromatographic media without clogging. Although some proteins are denatured by this procedure, most of the water-soluble proteins remain in solution, including biotin-binding protein and riboflavin-binding protein.
Department of Chemistry, University of Delaware, Newark 19711, USA.
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D020001:1-Butanol; D000818:Animals; D002645:Chickens; D002855:Chromatography, Thin Layer; D004527:Egg Proteins / Q000302:isolation & purification*; D007525:Isoelectric Focusing; D008055:Lipids / Q000302:isolation & purification; D012995:Solubility; D014461:Ultracentrifugation; D014867:Water
D004527:Egg Proteins; D008055:Lipids; D014867:Water; D020001:1-Butanol