Overexpression of human intestinal oligopeptide transporter in mammalian cells via adenoviral transduction.
OBJECTIVE
Our goals are to establish an in vitro screening system and to evaluate a new approach in improving oral absorption of peptides and peptide-like drugs by overexpression of the human intestinal oligopeptide transporter (hPepT1). This study characterizes the expression of hPepT1 in human intestinal Caco-2 cells, rat intestinal epithelial cells (IEC-18), and human cervix epithelial cells (Hela) after adenoviral transduction.


METHODS
A recombinant replication-deficient adenovirus carrying the hPepT1 gene was made and used as a vector for the expression of hPepT1. The increase in the uptake permeability of cephalexin and Gly-Sar was determined. The effects of time, dose, apical pH, and substrate specificity were evaluated.


RESULTS
A significant increase in the uptake permeability of Gly-Sar and cephalexin was found in all three cell lines after viral transduction. The increase of Gly-Sar permeability in Hela. IEC-18, and Caco-2 cells was 85-, 46-, and 15-fold respectively. Immunoblotting using an antibody against hPepT1 detected high levels of a 85-98-kDa protein in all three infected cell lines. Substrate permeability was dependent on time of infection, inward pH gradients, and multiplicity of infection (MOI). Decreased infectivity and lower hPepT1 expression were observed in differentiated Caco-2 cells. The uptake was inhibited by dipeptides and beta-lactam antibiotics but not amino acids.


CONCLUSIONS
Adenoviral infected Hela cells displayed a pronounced level of hPepT1 expression with a low background and high specificity to dipeptides. These features make this system a useful tool for screening of potential substrates. The success of overexpression of hPepT1 in Caco-2 and IEC-18 cells may lead to a novel approach in improving oral absorption of peptides and peptidornimetic drugs.
College of Pharmacy, The University of Michigan, Ann Arbor 48109-1065, USA.
C P
CP
Hsu
J M
JM
Hilfinger
E
E
Walter
H P
HP
Merkle
B J
BJ
Roessler
G L
GL
Amidon
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D015153:Blotting, Western; D018938:Caco-2 Cells; D015820:Cadherins; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D026901:Membrane Transport Proteins; D051381:Rats; D013379:Substrate Specificity; D013997:Time Factors
C490584:CDH17 protein, human; D015820:Cadherins; D002352:Carrier Proteins; C490586:Cdh17 protein, rat; D026901:Membrane Transport Proteins; C067802:intestinal peptide-proton cotransporter