A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M.
Cell cycle progression is monitored by highly coordinated checkpoint machinery, which is activated to induce cell cycle arrest until defects like DNA damage are corrected. We have isolated an anti-proliferative cell cycle regulator named G2A (for G2 accumulation), which is predominantly expressed in immature T and B lymphocyte progenitors and is a member of the seven membrane-spanning G protein-coupled receptor family. G2A overexpression attenuates the transformation potential of BCR-ABL and other oncogenes, and leads to accumulation of cells at G2/M independently of p53 and c-Abl. G2A can be induced in lymphocytes and to a lesser extent in nonlymphocyte cell lines or tissues by multiple stimuli including different classes of DNA-damaging agents and serves as a response to damage and cellular stimulation which functions to slow cell cycle progression.
Weng
Z
Z
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Fluckiger
A C
AC
Nisitani
S
S
Wahl
M I
MI
Le
L Q
LQ
Hunter
C A
CA
Fernal
A A
AA
Le Beau
M M
MM
Witte
O N
ON
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004249:DNA Damage; D017931:DNA Primers; D004261:DNA Replication; D016195:G2 Phase; D019204:GTP-Binding Proteins / Q000378:metabolism*; D051379:Mice; D008938:Mitosis; D008969:Molecular Sequence Data; D018384:Oxidative Stress; D051381:Rats; D043562:Receptors, G-Protein-Coupled; D015398:Signal Transduction
D018797:Cell Cycle Proteins; D017931:DNA Primers; C115270:G2A receptor; D043562:Receptors, G-Protein-Coupled; D019204:GTP-Binding Proteins