In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification.
OBJECTIVE
The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH).


METHODS
Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH.


RESULTS
CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology.


CONCLUSIONS
CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.
Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
P T
PT
Odinot
J F
JF
Meis
J A
JA
Hoogkamp-Korstanje
W J
WJ
Melchers
D000818:Animals; D018938:Caco-2 Cells; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D017403:In Situ Hybridization; D051381:Rats; D011916:Rats, Inbred F344; D015009:Yersinia Infections / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000302:isolation & purification*