[Suppression of transforming growth factor beta 1 on malignant phenotype in HL-60 cells].
OBJECTIVE
To study the effect of transforming growth factor beta 1 on tumor cells.


METHODS
HL-60 cells were transfected with transforming growth factor beta 1 expression vector by electroporation. Stable cell clones resistant to G 418 were obtained after 2-3 week growth with the drug-containing medium. A series of malignant phenotype were compared between transfected and untransfected HL 60 cells.


RESULTS
The growth speed of HL-60 cells which overexpressed transforming growth factor beta 1 mRNA decreased significantly. Clonigenecity in soft agarase and tumor formation in athymic mice were lower than those untransfected HL-60 cells.


CONCLUSIONS
Suppression effect of transforming growth factor beta 1 on malignant phenotype in HL-60 cells were demonstrated.
Institute for Environmental Surveillance, Chinese Academy of Preventive Medicine, Beijing.
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L
Song
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Bai
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Yang
D000818:Animals; D002455:Cell Division; D018274:Electroporation; D016147:Genes, Tumor Suppressor; D018922:HL-60 Cells / Q000473:pathology*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta