Rapid analysis of allelic variants of the sheep PrP gene by oligonucleotide probes.
A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP(MARQ), PrP(TARQ) and PrP(MARR), were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171-Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie-resistant progenitors in the flocks.
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan. ishiguro@obihiro.ac.jp
N
N
Ishiguro
M
M
Shinagawa
S
S
Onoe
K
K
Yamanouchi
T
T
Saito
D000483:Alleles; D000818:Animals; D004252:DNA Mutational Analysis; D004198:Disease Susceptibility; D005260:Female; D014644:Genetic Variation; D008297:Male; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018620:PrPSc Proteins / Q000235:genetics*; D012608:Scrapie / Q000235:genetics*; D012756:Sheep / Q000235:genetics*
D015345:Oligonucleotide Probes; D018620:PrPSc Proteins