Effect of CRF and related peptides on calcium signaling in human and rodent melanoma cells.
Corticotropin releasing factor (CRF) induces a rapid, within seconds, and dose-dependent increase in the intracellular Ca2+ in both human and hamster melanoma cells. This effect is inhibited by depletion of extracellular calcium using 3 mM EGTA and is attenuated by the CRF receptor antagonist, alpha-helical-CRF(9-41). Other peptides of the CRF superfamily, sauvagine and urocortin, also induce increases in cytoplasmic calcium concentration but at higher concentrations than CRF. We conclude that malignant melanocytes express CRF receptors, which are coupled to activation of plasma membrane calcium channels.
Department of Pathology, Medical Center, Loyola University, Maywood, IL 60153, USA.
N
N
Fazal
A
A
Slominski
M A
MA
Choudhry
E T
ET
Wei
M M
MM
Sayeed
D029845:Amphibian Proteins; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000494:pharmacology*; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D036361:Peptide Hormones; D010455:Peptides / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects*; D014407:Tumor Cells, Cultured; D054832:Urocortins; D014665:Vasodilator Agents / Q000494:pharmacology
D029845:Amphibian Proteins; D036361:Peptide Hormones; D010455:Peptides; D054832:Urocortins; D014665:Vasodilator Agents; C025727:sauvagine; D003346:Corticotropin-Releasing Hormone; D002118:Calcium