A new method to characterize saturation functions by their first four moments.
The calculation of the first four moments of saturation functions is proposed as a method to describe the properties of enzymes or receptors models. The values of these moments in the case of the Langmuir or Michaelis-Menten equation and the Hill equation are reviewed. They have been calculated for the second degree Adair equation and in the case of binding site heterogeneity. A method for generalization to cases of greater complexity is also proposed. The advantage of this method over the classical ones--graphical representations and derivation of coefficients like nH, [L]0.9/[L]0.1 ...--is essentially that the moments are defined by one single value independently of any particular model for the whole of the saturation curve.
Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.
J M
JM
Boeynaems
F R
FR
Cantraine
D001665:Binding Sites; D001699:Biometry; D004798:Enzymes / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D016015:Logistic Models; D008954:Models, Biological; D011485:Protein Binding
D004798:Enzymes; D008024:Ligands