[An antigenic analysis and the protective properties of the R-form lipopolysaccharides of gram-negative bacteria].
The antigenic and immunogenic properties of the R-form lipopolysaccharides (R-LPS) of Pseudomonas aeruginosa, Salmonella minnesota, Escherichia coli and Shigella were studied. The results of the study revealed the existence of antigenic relationship between P.aeruginosa R-LPS and R-LPS Escherichia and Shigella. In serological tests no antigenic relationship between P. aeruginosa R-LPS and Salmonella R-LPS was revealed, but as shown in earlier experiments of the protection of mice, Re-glycolipid stimulated protective immunity against Pseudomonas infection in the animals. On the basis of R-LPS obtained from selected P. aeruginosa and Salmonella strains a vaccine was prepared which proved to be effective against infection caused by P. aeruginosa S-strain in experiments on mice. The vaccine induced protection in 40-100% of immunized mice, depending on the scheme of immunization. The vaccine may probably be effective against infections caused by other gram-negative bacteria.
Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia.
E S
ES
Stanislavskiĭ
T A
TA
Makarenko
E V
EV
Kholodkova
C
C
Lugowski
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology*; D001428:Bacterial Vaccines / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004926:Escherichia coli / Q000276:immunology; D006090:Gram-Negative Bacteria / Q000276:immunology*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control; D007114:Immunization; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D009154:Mutation / Q000276:immunology; D016981:Plesiomonas / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology; D012475:Salmonella / Q000276:immunology; D012764:Shigella sonnei / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides
Antigennyĭ analiz i protektivnye svoĭstva lipopolisakharidov R-form nekotorykh gramotritsatel'nykh bakteriĭ.