Primary intestinal epithelial cells can be infected with laboratory-adapted strain HIV type 1 NDK but not with clinical primary isolates.
Infectivities of HIV-1 primary isolates and laboratory-adapted strains were compared in primary fetal enterocytes and the colonic epithelial cell line HT29. Infection by two laboratory strains, HIV-1 NDK and HIV-1 NDK(A4), which were adapted on CEM and HT29 cells, respectively, produced significant amounts of virus in both target cell systems. Intestinal cells were resistant to infection with HIV-1 primary isolates regardless of their genetic subtype or SI/NSI phenotype. Biological properties of analyzed viruses rather than differences in cultivation system seem to be responsible for differences between these in vitro and ex vivo results.
INSERM U322, Unité de Recherches sur les Rétrovirus et maladies associées, Marseille, France.
A L
AL
Chenine
E
E
Matouskova
G
G
Sanchez
J
J
Reischig
L
L
Pavlikova
C
C
LeContel
J C
JC
Chermann
I
I
Hirsch
D002478:Cells, Cultured; D004848:Epithelium / Q000821:virology; D005260:Female; D005333:Fetus; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000502:physiology; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology* / Q000821:virology*; D007633:Keratins / Q000032:analysis; D010641:Phenotype; D011247:Pregnancy
D007633:Keratins