Function of zebrafish beta-catenin and TCF-3 in dorsoventral patterning.
We report the molecular cloning and expression of the zebrafish tcf-3 homologue and study its function and that of zebrafish betacat in dorsoventral patterning. Overexpression of mutant zTcf-3 products and Cadherin leads to a reduction in the expression of the dorsal-specific genes goosecoid and chording at the blastula stages, indicating a conserved role for betacat and tcf-3 in zebrafish dorsal axis induction. Later during gastrulation, overexpression of these same products leads to the ectopic expression of dorsal-specific genes in the marginal zone and the induction of ectopic axes, suggesting an additional role for betacat and Tcf-3 at these later stages in the repression of dorsal fates.
Max-Planck Institut fur Entwicklungsbiologie, Spemannstrasse 35/111, 72076, Tübingen, Germany. pel@mailer.mpib-tuebingen.mpg.de
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D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001665:Binding Sites / Q000235:genetics; D019521:Body Patterning / Q000235:genetics / Q000502:physiology*; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000502:physiology*; D029721:Drosophila Proteins; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D005775:Gastrula / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005796:Genes / Q000235:genetics; D024002:HMGB Proteins; D018398:Homeodomain Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D017386:Sequence Homology, Amino Acid; D051820:TCF Transcription Factors; D015534:Trans-Activators; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors / Q000235:genetics / Q000502:physiology*; D015027:Zebrafish / Q000196:embryology* / Q000235:genetics; D029961:Zebrafish Proteins; D051176:beta Catenin
D001426:Bacterial Proteins; D003598:Cytoskeletal Proteins; D029721:Drosophila Proteins; D024002:HMGB Proteins; D018398:Homeodomain Proteins; D051820:TCF Transcription Factors; D015534:Trans-Activators; D058125:Transcription Factor 7-Like 1 Protein; D014157:Transcription Factors; D029961:Zebrafish Proteins; D051176:beta Catenin; C495269:ctnnb1 protein, zebrafish; C052837:eve protein, Drosophila