Interference by carbohydrate substrates, flavonoids, and monosaccharide derivatives on bacterial beta-D-glucuronidase assays.
Most commercially available test kits for water and foodstuffs use beta-galactosidase activity for coliforms and beta-glucuronidase activity for Escherichia coli. We tested the effects on the beta-glucuronidase activity of E. coli W3110 of substances usually present in foods and several synthetic pharmaceutical compounds. Thirteen substances were tested: three carbohydrates, four flavonoids, five monosaccharide derivatives, and dimethyl sulphoxide. In a minimum medium without any other carbon source, glucose (0.1 mM), quercetin (0.1 mM), silymarin (10 mg/L), D-gluconic acid (0.01 mM), D-gluconic acid lactone (0.01 mM), isopropyl-beta-D-thiogalacto pyranoside (1 mM), p-nitrophenyl beta-D-glucuronide (1 mM), and DMSO (1 M) completely inhibited E. coli glucuronidase activity at the above concentrations. However, the following compounds stimulated E. coli glucuronidase activity within the ranges of concentrations shown: glucose (0.0001-0.01 mM), lactose and sucrose (>0.1 mM), D-saccharic acid 1,4 lactone (0.0001-0.1 mM), p-nitrophenyl beta-D-glucuronide (0.001-0.01 mM) and DMSO (2-500 mM). In a rich culture medium that contained other carbon sources (lauryl tryptose broth) E. coli glucuronidase activity in the presence of the extra nutrients was unaffected by the test substances and therefore, under normal conditions in water or foods, they should not interfere with E. coli assays based on measurements of beta-glucuronidase activity.
Departament of Preventive Medicine and Public Health, School of Medecine, University of Malaga, Campus Teatinos, 29071 Malaga, Spain.
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A
Mariscal
J
J
Gómez-Aracena
M C
MC
Varo
J
J
Fernández-Crehuet
D001681:Biological Assay; D050260:Carbohydrate Metabolism; D004926:Escherichia coli / Q000201:enzymology*; D005420:Flavoproteins / Q000378:metabolism; D005506:Food Contamination; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D009005:Monosaccharides / Q000378:metabolism; D004364:Pharmaceutical Preparations
D005420:Flavoproteins; D009005:Monosaccharides; D004364:Pharmaceutical Preparations; D005966:Glucuronidase