New antiherpetic nucleoside from a Basidiomycete.
Antiviral activity was characterized from the culture broth of the Basidiomycete Macrocystidia cucumis (Pers. ex Fr.) Heim. When the stationary phase was reached (21 d), the culture broth was shown through an ELISA assay to contain antiviral activity against herpes simplex virus type 1 (HSV-1), as assessed in baby hamster kidney cells (BHK-21). Once the presence of the anti-HSV-1 activity in the culture broth was demonstrated, we proceeded with the purification and isolation of the active principle using a semi-preparative HPLC technique. The activity was associated with a purine nucleoside designated McA. This compound displayed no cytotoxicity at antivirally effective concentrations and proved to be a novel nucleoside analogue.
Laboratoire de mycologie appliquée aux biotechnologies industrielles, Institut des sciences pharmaceutiques et biologiques de Lyon, université Claude-Bernard-Lyon-I, France.
D
D
Saboulard
A
A
Gaspar
B
B
Roussel
J
J
Villard
D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001487:Basidiomycota / Q000737:chemistry*; D001681:Biological Assay; D002460:Cell Line; D006224:Cricetinae; D008826:Microbial Sensitivity Tests; D009705:Nucleosides / Q000302:isolation & purification* / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development
D000998:Antiviral Agents; D009705:Nucleosides