Suppressive effects of SP-A on ionomycin-induced IL-8 production and release by eosinophils.
Recent studies have demonstrated that pulmonary surfactant protein (SP)-A plays a potential role in modifying inflammation and immune function. To see whether SP-A could modify IL-8 production and release by eosinophils stimulated with ionomycin, SP-A purified from surfactant recovered from patients with alveolar proteinosis was added to eosinophils isolated by the negative-selection method with immunomagnetic beads, and cultured for 24 h. The concentrations of IL-8 in the cell-free supernatants and cell lysates were then measured by ELISA. SP-A attenuated the production of IL-8 by eosinophils in a concentration-dependent manner. SP-A also attenuated the release of IL-8 from the eosinophils. The addition of SP-A antibody (PE10) reversed these effects of SP-A completely. These data suggest that SP-A may have the potential to modify allergic inflammation by inhibiting the release and production of IL-8 by eosinophils.
Department of Internal Medicine and Clinical Immunology, Dokkyo University School of Medicine, Tochigi, Japan.
G
G
Cheng
T
T
Ueda
H
H
Nakajima
A
A
Nakajima
S
S
Kinjyo
S
S
Motojima
T
T
Fukuda
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001249:Asthma / Q000188:drug therapy / Q000209:etiology / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D004804:Eosinophils / Q000187:drug effects* / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy / Q000209:etiology / Q000276:immunology; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000378:metabolism*; D015759:Ionomycin / Q000494:pharmacology*; D008168:Lung / Q000276:immunology / Q000378:metabolism; D011510:Proteolipids / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants / Q000008:administration & dosage / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D000911:Antibodies, Monoclonal; D016209:Interleukin-8; D011510:Proteolipids; D037662:Pulmonary Surfactant-Associated Protein A; D037661:Pulmonary Surfactant-Associated Proteins; D011663:Pulmonary Surfactants; D015759:Ionomycin