[Correlation between glucose and lactate concentrations in the human cornea and in organ culture medium].
BACKGROUND
Metabolic changes of the human donor cornea during organ-culture are not at all reflected by the endothelium. Therefore metabolic investigations have become of increasing interest. It was the aim of this study to determine the correlation between glucose and lactate in storage medium and within the cornea itself and to find thereby an additional parameter for glucose metabolism during organ-culture.


METHODS
Glucose and lactate were examined in 166 organ-culture medium samples as well as in 106 human corneas by enzymatical optical methods. Investigations were carried out after 1, 7, 15, 21 and 28 days of organ-culture.


RESULTS
Glucose consumption was highest during the first two weeks of organ-culture. Glucose concentrations showed a good linear correlation between medium samples and the cornea (r = 0.923). The correlation coefficient for lactate was worse (r = 0.733).


CONCLUSIONS
Glucose and lactate levels in the organ-culture medium can be used as a marker for glucose metabolism in the cornea.
Augenklinik der RWTH Aachen.
C
C
Redbrake
A
A
Frantz
S
S
Salla
D001786:Blood Glucose / Q000378:metabolism*; D003315:Cornea / Q000378:metabolism*; D016039:Corneal Transplantation / Q000502:physiology*; D003470:Culture Media; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D009926:Organ Preservation; D014022:Tissue Survival / Q000502:physiology
D001786:Blood Glucose; D003470:Culture Media; D019344:Lactic Acid
Korrelation zwischen Glucose- und Laktatkonzentrationen in der humanen Hornhaut und im Organkulturmedium.