Consecutive in vivo measurement of nitric oxide in transient forebrain ischemic rat under normothermia and hypothermia.
The effects of hypothermia on production of nitric oxide (NO) in ischemic brain were investigated by using in vivo microdialysis. Male Wistar rats were randomly divided into three groups; saline-treated normothermic group (37 degreesC, n=6), 30 mg/kg N-nitro-l-arginine methyl ester(l-NAME)-treated normothermic group (n=6), and saline-treated hypothermic group (30 degreesC, n=6). Transient forebrain ischemia was produced by bilateral common carotid artery occlusion combined with hypotension (MABP=50 mmHg). Saline-treated normothermic animals resulted in a reduction of LCBF to 9% of baseline. Saline-treated hypothermic rats revealed the similar changes of LCBF. In contrast, l-NAME administration reduced the basal CBF to 85% of saline-treated group and to 8% after ischemia. NO products were decreased during ischemia and transiently increased after reperfusion in saline-treated groups. However, the increase of NO products after reperfusion was less significant in the hypothermia. l-NAME-treated group showed a constant reduction of NO production during ischemia and after reperfusion.
Department of Neurosurgery, Asahikawa Medical College, 4-5 Nishikagura, Asahikawa, 078, Japan.
T
T
Sugimura
K
K
Sako
Y
Y
Tohyama
Y
Y
Yonemasu
D000818:Animals; D001831:Body Temperature / Q000502:physiology; D001921:Brain / Q000098:blood supply / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects; D002560:Cerebrovascular Circulation / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D007036:Hypothermia, Induced; D002546:Ischemic Attack, Transient / Q000378:metabolism* / Q000628:therapy*; D008297:Male; D017551:Microdialysis; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D018696:Neuroprotective Agents / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy
D004791:Enzyme Inhibitors; D018696:Neuroprotective Agents; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester