The genomic organization of the human corticotropin-releasing factor type-1 receptor.
We determined the genomic organization of human CRF type-1 receptor (hCRF-R1). The gene coding for hCRF-R1 consists of at least 14 exons and spans over 20 kilobases. hCRF-R1's three reported isoforms originate from the same gene by alternative splicing. The first hCRF-R1, which binds to CRF with the highest affinity and transduces the most sensitive cAMP accumulation in response to CRF, is encoded in a total of 13 exons, the only one excluded being exon 6. The second isoform contains an additional 29-amino acid sequence which corresponds to exon 6. Unlike the first isoform, the third lacks a 40-amino acid sequence, corresponding to exon 3. Exon-intron boundaries are the same as that of the consensus sequence. Locations of introns in the coding sequence are similar to human CRF-R1, rat CRF-R1, human CRF-R2alpha and others belonging to the human glucagon receptor family.
Sakai
K
K
Third Division, Department of Medicine, Hirosaki University School of Medicine, Hirosaki 036, Japan. yokos@cc.hirosaki-u.ac.jp
Yamada
M
M
Horiba
N
N
Wakui
M
M
Demura
H
H
Suda
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D005091:Exons; D005796:Genes; D015698:Genomic Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D051381:Rats; D018019:Receptors, Corticotropin-Releasing Hormone / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D000099052:CRF Receptor, Type 1
D018019:Receptors, Corticotropin-Releasing Hormone; D000099052:CRF Receptor, Type 1; D003346:Corticotropin-Releasing Hormone