Mechanisms of gene expression controlled by pressure in deep-sea microorganisms.
A pressure-regulated operon has been cloned and sequenced from deep-sea barophilic Shewanella strains. To understand pressure-regulated mechanisms of gene expression, a regulatory element upstream of the pressure-regulated operon from Shewanella sp. strain DSS12 was studied. Regions A and B were classified by sequence analysis. A unique octamer motif, AAGGTAAG, was found to be repeated in tandem 13 times in region B. An electrophoretic mobility shift assay demonstrated that a O54-like factor recognizes region A and other unknown factors recognize region B. Different shift patterns of the protein-DNA complexes were observed when extracts of cells cultured at 0.1 MPa or 50 MPa were incubated with a DNA probe specific for region B. These results indicate that the deep-sea strain DSS12 expresses different DNA-binding factors under different pressure conditions.
Japan Marine Science and Technology Center, Yokosuka, Japan.
K
K
Nakasone
A
A
Ikegami
C
C
Kato
R
R
Usami
K
K
Horikoshi
D001426:Bacterial Proteins / Q000378:metabolism; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D009876:Operon; D011312:Pressure; D012091:Repetitive Sequences, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins