CD38 expression on cryopreserved CD8+ T cells predicts HIV disease progression.
Previous studies have revealed that the expression of CD38 on CD8+ T cells is a strong predictor of disease progression in human immunodeficiency virus (HIV)-infected individuals. Those studies were performed using fresh patient samples over an extended trial period. After demonstrating the validity of assay results on cryopreserved cells, we performed a retrospective study using frozen cell samples to determine the predictive value of CD38 expression in patients with CD4 counts above 400 cells/microl. The CD38 expression as measured by antibody binding capacity and the CD38 median channel were shown to be associated with time to new opportunistic infection or death (both P < 0.001). These results suggest that CD38 expression on CD8+ T cells, whether fresh or frozen, provides a useful predictor of HIV disease progression.
Perfetto
S P
SP
Henry M. Jackson Foundation, Rockville, Maryland, USA.
Malone
J D
JD
Hawkes
C
C
McCrary
G
G
August
B
B
Zhou
S
S
Garner
R
R
Dolan
M J
MJ
Brown
A E
AE
D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000328:Adult; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis*; D015415:Biomarkers; D001793:Blood Preservation; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry*; D015925:Cryopreservation; D018450:Disease Progression; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000473:pathology; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins; D008875:Middle Aged; D009244:NAD+ Nucleosidase / Q000032:analysis*
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015415:Biomarkers; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1