Cloning of the porcine interferon-gamma receptor and its foeto-endometrial expression in early pregnancy.
In early gestation, trophoblastic cells of porcine preimplanting conceptuses transiently and massively secrete two distinct interferons (IFNs), one of which is IFN-gamma. In order to localize possible cellular target(s) for this IFN-gamma, the expression of the porcine IFN-gamma receptor and its developmental regulation have been investigated on the maternal endometrium and on the embryonic tissues. A cDNA encoding the porcine IFN-gamma binding-chain (pIFNGR1) was isolated. When expressed in COS-7 cells, it displayed a specific binding to radiolabelled pIFN-gamma and was shown to be a glycosylated membrane protein with an apparent molecular mass of 92 kDa. Porcine IFNGR1 mRNA was detected by RT-PCR not only in uterine epithelial cells but also in embryonic tissues from at least as early as day 10 of gestation. Moreover, membrane expression of the pIFN-gamma receptor quantified by binding and crosslinking of 32P-pIFN-gamma was demonstrated in uterine epithelium and in the trophoblast. In the trophoblast, expression of the receptor was found to be developmentally regulated: although expression was weak on days 12 and 15 of gestation, it reached a level similar to that found on some IFN-gamma-sensitive cells on day 16. This study shows that maternal endometrium is not the only possible target for trophoblastic IFN-gamma: the induction of pIFN-gamma receptor expression in the trophoblast around day 16 of gestation could suggest the appearance of responsiveness to pIFN-gamma in this implanted tissue and therefore a possible delayed autocrine effect of trophoblastic pIFN-gamma.
D'Andréa
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S
Institut National de la Recherche Agronomique, Laboratoire de Virologie et d'Immunologie Moléculaires, Jouy-en-Josas, France.
La Bonnardière
C
C
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D004717:Endometrium / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D007371:Interferon-gamma / Q000378:metabolism; D008969:Molecular Sequence Data; D011247:Pregnancy; D017471:Receptors, Interferon / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment; D013552:Swine; D000096743:Interferon gamma Receptor
D018076:DNA, Complementary; D007371:Interferon-gamma; D017471:Receptors, Interferon; D000096743:Interferon gamma Receptor