Glucose transport in a methylotrophic yeast Hansenula polymorpha.
Glucose transport was studied in a methylotrophic yeast Hansenula polymorpha. Two kinetically different glucose transport systems were revealed in cells grown under different growth conditions. Glucose-repressed cells exhibited a low-affinity transport system (Km for glucose 1.75 mM) while glucose-derepressed and ethanol-grown cells had a high-affinity transport system (Km for glucose 0.05-0.06 mM). The high- and low-affinity transport systems differed in substrate specificity, sensitivity to pH, dinitrophenol and protonophore carbonyl cyanide-m-chlorophenyl-hydrazone. The kinetic rearrangement of the glucose transport system in response to altered growth conditions was dependent on de novo protein synthesis.
Institute of Molecular and Cell Biology, University of Tartu, Estonia.
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Karp
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Alamäe
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D001692:Biological Transport; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010843:Pichia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013379:Substrate Specificity
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D005947:Glucose; D019297:2,4-Dinitrophenol