Purification, crystallization and preliminary crystallographic analysis of bovine cytosolic brain-type creatine kinase.
Creatine kinase (E.C. 2.3.7.2) is an important enzyme in energy metabolism which catalyzes the reversible transfer of a phosphoryl group between phosphocreatine and ADP to give ATP. Large quantities of a brain-type creatine kinase have been isolated from bovine photoreceptor cells and crystals suitable for X-ray diffraction analysis have been obtained by hanging-drop vapor diffusion. Crystals grow as tetragonal bipyramids in space group P43212 with cell dimensions a = b = 96.49, c = 108.42 A and diffract to at least 2.7 A resolution.
University of Illinois at Chicago, Department of Biochemistry and Molecular Biology, A-312 College of Medicine West, 1819 West Polk Street, Chicago, IL 60612-7334, USA. aloew@uic.edu
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D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002853:Chromatography, Liquid; D003402:Creatine Kinase / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D017886:Durapatite; D005136:Eye Proteins / Q000737:chemistry* / Q000302:isolation & purification; D007527:Isoenzymes; D009419:Nerve Tissue Proteins / Q000737:chemistry* / Q000302:isolation & purification; D011487:Protein Conformation; D012160:Retina / Q000201:enzymology*; D012685:Sepharose
D005136:Eye Proteins; D007527:Isoenzymes; D009419:Nerve Tissue Proteins; D012685:Sepharose; D017886:Durapatite; D003402:Creatine Kinase