Sulfalene concentrations in plasma and blood cells of Plasmodium falciparum malaria cases after treatment with metakelfin using high-performance liquid chromatography.
A reversed-phase high-performance liquid chromatographic method using acetonitrile-methanol-1 M perchloric acid-water (25:9:0.8:95, v/v/v) at a flow-rate of 1.0 ml min(-1) on LiChrospher 100 RP 18 column (250 x 4 mm; 5 microm) with UV (254 nm) detection has been developed for the determination of sulfalene in plasma and blood cells after oral administration of the antimalarial drug metakelfin. Calibration curves were linear in the range 0.5-100 microg ml(-1). The limit of quantification was 50 ng ml(-1). Within-day and day-to-day coefficients of variation averaged 3.84 and 5.31%, respectively. Mean extraction recoveries of sulfalene from plasma and blood cells were 87.21 and 84.65%, respectively. Mean concentrations of sulfalene in plasma of P. falciparum cases on days 2, 7 and 15 were 44.58, 14.90 and 1.70 microg ml(-1), respectively; in blood cells concentrations of sulfalene were 7.77, 3.25 and 0.75 microg ml(-1), respectively, after oral treatment with two tablets (1000 mg) of metakelfin. Significant difference was recorded on day 2 for sulfalene concentration in blood cells of healthy and P. falciparum cases (t=9.49; P<0.001).
Malaria Research Centre (Field Station), Health Centre, Sctor III, BHEL, Ranipur, Hardwar, India.
V K
VK
Dua
R
R
Sarin
N C
NC
Gupta
V P
VP
Sharma
D000328:Adult; D000962:Antimalarials / Q000627:therapeutic use*; D001773:Blood Cells / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004338:Drug Combinations; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000097:blood / Q000188:drug therapy*; D008297:Male; D008875:Middle Aged; D011739:Pyrimethamine / Q000627:therapeutic use*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013415:Sulfalene / Q000097:blood* / Q000627:therapeutic use*
D000962:Antimalarials; D004338:Drug Combinations; C050416:metakelfin; D013415:Sulfalene; D011739:Pyrimethamine