Whole sequence of spoIIIE-like, sporulation-inhibitory, and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus and detection of spi-like gene in the actinomycete chromosome.
The nucleotide sequence of spoIIIE-like and the sporulation-inhibitory and transfer gene (spi) in a conjugative plasmid, pSA1.1, of Streptomyces azureus were examined to detect the promoter region. Using Southern blotting and a spi fragment as probe, spi-like genes were detected in chromosomes of the host and other actinomycetes. These results suggest that there is a spi- and spoIIIE-like gene in chromosomes of some actinomycetes.
Microbial Genetics Division, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
K
K
Doi
Y
Y
Ono
E
E
Yokoyama
Y
Y
Tsukagoe
S
S
Ogata
D000192:Actinomycetales / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial / Q000648:ultrastructure; D003227:Conjugation, Genetic / Q000235:genetics*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000737:chemistry; D011401:Promoter Regions, Genetic / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000737:chemistry; D013302:Streptomyces / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors
D001426:Bacterial Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C114917:spi protein, actinomycetes; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus