The relationship between microbial metabolic activity and biocorrosion of carbon steel.
The effect of metabolic activity (expressed by generation time, rate of H2S production and the activity of hydrogenase and adenosine phosphosulphate (APS)-reductase enzymes) of the 8 wild strains of Desulfovibrio desulfuricans and of their resistance to metal ions (Hg2+, Cu2+, Mn2+, Zn2+, Ni2+, Cr3+) on the rate of corrosion of carbon steel was studied. The medium containing lactate as the carbon source and sulphate as the electron acceptor was used for bacterial metabolic activity examination and in corrosive assays. Bacterial growth inhibition by metal ions was investigated in the sulphate-free medium. The rate of H2S production was approximately directly proportional to the specific activities of the investigated enzymes. These activities were inversely proportional to the generation time. The rate of microbiologically induced corrosion (MIC) of carbon steel was directly proportional to bacterial resistance to metal ions (correlation coefficient r = 0.95). The correlation between the MIC rate and the activity of enzymes tested, although weaker, was also observed (r = 0.41 for APS-reductase; r = 0.69 for hydrogenase; critical value rc = 0.30, p = 0.05, n = 40).
Department of Biochemistry and Biophysics, Silesian Medical Academy, Sosnowiec, Poland.
Z
Z
Dzierzewicz
B
B
Cwalina
E
E
Chodurek
T
T
Wilczok
D003343:Corrosion; D003901:Desulfovibrio / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006862:Hydrogen Sulfide / Q000378:metabolism; D006864:Hydrogenase / Q000378:metabolism; D008670:Metals / Q000008:administration & dosage / Q000494:pharmacology*; D010088:Oxidoreductases / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors; D013232:Steel
D008670:Metals; D013232:Steel; D010088:Oxidoreductases; D006864:Hydrogenase; D050862:Oxidoreductases Acting on Sulfur Group Donors; C019618:adenylylsulfate reductase; D006862:Hydrogen Sulfide