Long-term pharmacological kindling increases in vitro release of IR-Met and IR-Leu-enkephalin from amygdala.
Met-enkephalin release is increased from amygdala and striatum 1 and 15 days after pharmacological kindling with pentylenetetrazol, following potassium-induced depolarization in vitro via a Ca2+ dependent mechanism. Leu-enkephalin release was only enhanced in amygdala and striatum 1 day after the last seizure. IR-Met-enkephalin amygdala tissue content enhanced 1 and 15 days after seizure. In striatum, we found an IR-Met-enkephalin decrease 35 days after the last stimulus. IR-Leu-enkephalin amygdala tissue content enhanced 1 day after the last seizure, and no significant increases were found in striatum 1, 15 and 35 days after the last seizure. In this paper, we show that opioid peptides release is differentially enhanced in rat brain for several days after the last seizure, thus suggesting that opioid peptides may have a protective action against seizure activity.
Laboratorio de Análisis Químicos, Instituto Mexicano de Psiquiatría, Mexico D.F.
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Asai
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Matamoros-Trejo
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Linares
D000679:Amygdala / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D003292:Convulsants / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000378:metabolism*; D004744:Enkephalin, Methionine / Q000378:metabolism*; D066298:In Vitro Techniques; D007696:Kindling, Neurologic / Q000187:drug effects*; D008297:Male; D010433:Pentylenetetrazole / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D003292:Convulsants; D004744:Enkephalin, Methionine; D004743:Enkephalin, Leucine; D010433:Pentylenetetrazole