Activation of a consensus FNR-dependent promoter by DNR of Pseudomonas aeruginosa in response to nitrite.
Expression of the enzymes for anaerobic nitrate respiration of Pseudomonas aeruginosa requires two CRP/FNR-related transcriptional regulators, ANR and DNR. Activity of the consensus CRP- or FNR-dependent promoter in the anr and dnr deficient mutants was investigated. The CRP-dependent promoter was active in the mutant strains. Both regulators could activate the promoter with a consensus FNR-binding motif. DNR-dependent activation was nitrite-dependent, whereas activation by ANR was not, suggesting that only DNR is involved in sensing nitrogen oxides.
Department of Biotechnology, University of Tokyo, Japan.
N
N
Hasegawa
H
H
Arai
Y
Y
Igarashi
D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000378:metabolism*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007506:Iron-Sulfur Proteins / Q000378:metabolism*; D009154:Mutation; D009573:Nitrites / Q000378:metabolism / Q000494:pharmacology*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000187:drug effects*
D001426:Bacterial Proteins; C095475:DNR protein, Pseudomonas aeruginosa; D029968:Escherichia coli Proteins; C060704:FNR protein, E coli; D007506:Iron-Sulfur Proteins; D009573:Nitrites; D014157:Transcription Factors