Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers.
This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.
Munford
R S
RS
Department of Internal Medicine, University of Texas Health Science Center, Southwestern Medical School, Dallas 75235, USA.
D000818:Animals; D001798:Blood Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006737:Horseshoe Crabs; D007457:Iodine Radioisotopes; D008033:Limulus Test / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis*
D001798:Blood Proteins; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; C013445:coagulogen