Effects of various N-terminal fragments of glucagon-like peptide-1(7-36) on food intake in the neonatal chick.
Recently, the suppressive effect on food intake by the central administration of glucagon-like peptide-1 (GLP-1) has been confirmed in both rats and chicks. The importance of the N-terminal amino acid, histidine, for the bioactivity of GLP-1(7-36) in the central nervous system was suggested, though the role for C-terminal amino acids in the central nervous system has not been reported. The present study was done to elucidate the central effect of N-terminal fragments of GLP-1(7-36) on food intake of the neonatal chick. Intracerebroventricular (i.c.v.) administration of mammalian GLP-1(7-36) inhibited food intake of chicks, but the fragments of GLP-1(7-16) and GLP-1(7-26) did not show the suppressive effect on food intake. Furthermore, the extended fragments, GLP-1(7-30) and GLP-1(7-33), also had no effects on food intake. It is concluded that C-terminal amino acids of GLP-1(7-36) have an important role for the bioactivity in the central nervous system with special reference to feeding behavior.
Furuse
M
M
Laboratory of Animal Feed Science, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan. furuse@agr.kyushu-u.ac.jp
Bungo
T
T
Shimojo
M
M
Masuda
Y
Y
Saito
N
N
Hasegawa
S
S
Sugahara
K
K
D000595:Amino Acid Sequence; D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D002645:Chickens; D005247:Feeding Behavior / Q000187:drug effects*; D005934:Glucagon; D052216:Glucagon-Like Peptide 1; D004763:Glucagon-Like Peptides; D007276:Injections, Intraventricular; D008297:Male; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000494:pharmacology*; D017386:Sequence Homology, Amino Acid
D010446:Peptide Fragments; C061970:glucagon-like peptide 1 (7-36); D004763:Glucagon-Like Peptides; D052216:Glucagon-Like Peptide 1; D005934:Glucagon