Equine peripheral blood mononuclear cells proliferate in response to tetanus toxoid antigen.
It has been reported that equine peripheral blood mononuclear cells (PBMNs) do not proliferate in response to tetanus toxoid (TT) (Frayne and Stokes 1995, Research in Veterinary Science 59, 79-81). Here we demonstrate that lymphocyte proliferation responses to TT, which are characteristic of a recall antigen, may be achieved under certain culture conditions. Given that TT vaccination is routinely applied to many horses, TT is a suitable antigen for the investigation of cellular immune responses by peripheral blood mononuclear cells in the horse.
Department of Veterinary Basic Sciences, The Royal Veterinary College, Herts.
J
J
McKelvie
S
S
Little
A P
AP
Foster
F M
FM
Cunningham
A
A
Hamblin
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D006736:Horses; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D018655:Lymphocyte Count / Q000187:drug effects; D013745:Tetanus Toxoid / Q000276:immunology / Q000494:pharmacology*
D000942:Antigens, Bacterial; D013745:Tetanus Toxoid