Cloning, expression, and localization of a novel gamma-adaptin-like molecule.
We describe the cloning, expression, and localization of gamma2-adaptin, a novel isoform of gamma-adaptin. The predicted human and mouse gamma2-adaptin proteins are approximately 90 kDa and 64.4% and 61.7%) identical to gamma-adaptin, respectively. gamma2-Adaptin was localized to the Golgi, its localization distinct from gamma-adaptin. The membrane association of gamma- and gamma2-adaptin could further be distinguished by differential sensitivity to the fungal metabolite brefeldin A, gamma2-adaptin binding being insensitive to drug treatment. Together, these results suggest that gamma2-adaptin plays a role in membrane transport distinct from that played by gamma-adaptin.
Lewin
D A
DA
Yale University School of Medicine, Department of Cell Biology, New Haven, CT 06520-8002, USA.
Sheff
D
D
Ooi
C E
CE
Whitney
J A
JA
Yamamoto
E
E
Chicione
L M
LM
Webster
P
P
Bonifacino
J S
JS
Mellman
I
I
D033982:Adaptor Protein Complex gamma Subunits; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D009928:Organ Specificity; D017421:Sequence Analysis
D033982:Adaptor Protein Complex gamma Subunits; D008565:Membrane Proteins