Chromatographic separation and automated analysis of flavanols.
Flavanols from barley or hops were separated chromatographically and assayed automatically by reaction with the chromogen, 4-dimethylaminocinnamaldehyde. For separating the flavanols on Sephadex G-25, gradient elution with water-methanol mixtures was necessary. The chromogen was specific for flavanols and well suited to AutoAnalyzer methods. The method appears generally applicable in flavanol analysis of plant materials.
McMurrough
I
I
Research Laboratory, A. Guinness Son & Co. (Dublin) Ltd., St. James's Gate, Ireland.
McDowell
J
J
D002850:Chromatography, Gel / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D002934:Cinnamates; D003911:Dextrans; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D001467:Hordeum / Q000737:chemistry; D007202:Indicators and Reagents; D000432:Methanol; D014867:Water
D002934:Cinnamates; D003911:Dextrans; D005419:Flavonoids; D007202:Indicators and Reagents; D014867:Water; C025614:sephadex; C018523:4-dimethylaminocinnamaldehyde; D000432:Methanol