[Usefulness of micronucleus assay in radiosensitivity tests using human cancer cell lines].
BACKGROUND
Recently, micronucleus assay is expected to be one of the radiosensitivity tests. The usefulness of micronucleus assay was compared with MTT assay and clonogenic assay using 5 human derived urological cancer cell lines, NBT-2, T24, PC3, OS-RC-2, and RERF-LC-AI in vitro. The correlation between the results in vitro assay and the radiation effects of nude mouse in vivo was investigated.


METHODS
In vitro, the micronucleus frequency of 2 Gy radiation was scored in micronucleus assay. The survival fraction of 2 Gy radiation was obtained in MTT assay and clonogenic assay. The correlation between 3 assays was investigated. In vivo, cancer cells was inoculated to nude mouse and the tumor volume was measured at 3-7 days interval in control group and 10 Gy irradiated group. The tumor volume ratio in irradiated group to control group was calculated as a radiation effect in each cell lines, the correlation between this ratio in vivo and each value of 2 Gy radiation in vitro was studied.


RESULTS
The correlation between micronucleus frequency and survival fraction in clonogenic assay was statistically significant (r = 0.941, p = 0.0169). But the correlation of the survival fraction between MTT assay and clonogenic assay is not statistically significant. The correlation between micronucleus frequency and the tumor volume ratio in vivo was statistically significant (r = 0.990, p = 0.0011). The correlation between survival fraction in clonogenic assay and the tumor volume ratio in vivo was also statistically significant (r = 0.914, p = 0.0298). However, the correlation between survival fraction in MTT assay and the tumor volume ratio in vivo was not statistically significant (r = 0.782, p = 0.118).


CONCLUSIONS
In this 5 cell lines, micronucleus assay was most correlated to nude mouse radiation effect. This result suggested the possibility of micronucleus assay to be a better predictive method than clonogenic assay for radiosensitivity test.
Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical University.
H
H
Kimura
D000818:Animals; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D015162:Micronucleus Tests; D009368:Neoplasm Transplantation; D011836:Radiation Tolerance; D013778:Tetrazolium Salts; D013844:Thiazoles; D014407:Tumor Cells, Cultured / Q000528:radiation effects*; D014410:Tumor Stem Cell Assay; D014571:Urologic Neoplasms / Q000473:pathology / Q000532:radiotherapy
D013778:Tetrazolium Salts; D013844:Thiazoles