Cloning of the cDNA for glutamyl-tRNA synthetase from Arabidopsis thaliana.
We cloned and characterized a full-length cDNA that encodes a glutamyl-tRNA synthetase (GluRSAt) from Arabidopsis. The GluRSAt is coded by a single gene. A transcript of about 2.3 kb hybridized with the cDNA. The deduced protein from the cDNA contained 719 amino acids with an estimated molecular mass of 81 kDa. Expression of the GluRSAt in E. coli resulted in a protein of the expected size. Comparison of the amino acid sequence GluRSAt to other glutamyl-tRNA synthetases showed strong sequence similarity to cytoplasmic GluRS proteins.
Day
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Department of Biology and Program in Cell and Molecular Biology, Colorado State University, Fort Collins 80523, USA.
Golovkin
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Reddy
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D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D005975:Glutamate-tRNA Ligase / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; C114873:AT5G26710 protein, Arabidopsis; D005975:Glutamate-tRNA Ligase