The response of periodontal ligament cells to fibronectin.
Fibronectin (fn) is an extracellular matrix (ECM) molecule important in cell adhesion and migration and in wound healing. It is also likely important in periodontal ligament (PDL) cell-ECM interactions, and thus in regenerating periodontal tissues. In this study we characterized PDL cells and their interactions with FN, testing different PDL cell isolates taken from healthy and diseased conditions. PDL cells were characterized by their morphology, integrin profile, motility, and bone nodule formation. Cells were then assayed for adhesion, proliferation, and chemotaxis in response to FN or FN fragments. Cell isolates were morphologically heterogeneous and fibroblastic, had a normal-appearing actin cytoskeleton and a wide range of migration potentials, and formed bone-like nodules in vitro. They expressed alpha5, beta1, alpha v, and alpha4 integrin subunits, known receptors for FN, and in fact they bound FN preferentially at 5 and 10 microg/ml. Intact FN induced greater PDL cell proliferation and chemotaxis than did FN fragments (120-kDa cell-binding, 60-kDa heparin-binding, and 45-kDa collagen-binding). PDL cells harvested from diseased and healthy conditions were no different on the basis of these assays. These data demonstrate that PDL cells are a mixed population of fibroblastic cells, capable of forming a mineralized matrix. They also suggest that maximal proliferation and chemotaxis require specific FN domains that are present on the intact molecule but not its fragments.
Department of Stomatology, School of Dentistry, University of California San Francisco, 94143-0512, USA. ykapila@itsa.ucsf.edu
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Johnson
D000199:Actins / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000032:analysis; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D048429:Cell Size; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D005109:Extracellular Matrix / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D005353:Fibronectins / Q000494:pharmacology*; D006801:Humans; D039441:Integrin alpha4; D039482:Integrin alpha5; D039564:Integrin alphaV; D019012:Integrin beta1 / Q000032:analysis; D016023:Integrins / Q000032:analysis; D010012:Osteogenesis; D010510:Periodontal Diseases / Q000473:pathology; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D017469:Receptors, Fibronectin / Q000032:analysis; D012038:Regeneration; D014945:Wound Healing / Q000187:drug effects
D000199:Actins; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D005353:Fibronectins; D039482:Integrin alpha5; D039564:Integrin alphaV; D019012:Integrin beta1; D016023:Integrins; D017469:Receptors, Fibronectin; D039441:Integrin alpha4