[Comparison of the synonymous and non-synonymous substitution rates in various regions of the neuraminidase and hemagglutinin genes of the influenza A virus].
A new, statistically justified approach was used to estimate the synonymous and non-synonymous substitution rates in several antigenic variants of influenza-virus surface proteins. The rates were compared for antigenic and nonantigenic regions of neuraminidase and hemagglutinin, as well as for neuraminidase surface and internal amino acids identified by X-ray analysis. For neuraminidase, the estimation was performed for the first time. The non-synonymous substitution rate was shown to be significantly higher in antigenic than in nonantigenic sites. However, neither subsample of antigenic sites displayed a fixation rate of non-synonymous substitutions higher than that of synonymous substitutions, which would confirm the effect of positive selection on these sites and argue against a neutral evolution character. Specific features of methods used to estimate the substitution fixation rates and problems in their interpretation are discussed.
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
K S
KS
Makarova
Iu I
IuI
Wulf
E P
EP
Tereza
V A
VA
Ratner
D019943:Amino Acid Substitution; D000940:Antigenic Variation; D000956:Antigens, Viral / Q000737:chemistry*; D005814:Genes, Viral; D006388:Hemagglutinins / Q000235:genetics*; D009980:Influenza A virus / Q000235:genetics* / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics*
D000956:Antigens, Viral; D006388:Hemagglutinins; D009439:Neuraminidase
Sravnenie skorosti nakopleniia sinonimicheskikh i nesinonimicheskikh zamen dlia razlichnykh raĭonov genov neĭraminidazy i gemaggliutinina virusa grippa A.