cDNA excision in stable retroviral cDNA transfectants is prevented by R removal.
The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.
Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
R
R
Rosin-Arbesfeld
A
A
Gazit
A
A
Yaniv
D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D015321:Gene Rearrangement; D004860:Infectious Anemia Virus, Equine / Q000235:genetics*; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral