Restriction map of a YAC and cosmid contig encompassing the oculopharyngeal muscular dystrophy candidate region on chromosome 14q11.2-q13.
As part of our effort to clone positionally the oculopharyngeal muscular dystrophy (OPMD) gene, we constructed a YAC contig, a cosmid contig, and an EcoRI restriction map of the OPMD candidate region. The YAC contig spans more than 2 Mb and encompasses the loci D14S283 and D14S990 and the cardiac alpha and beta myosin heavy chain genes (MYH6 and MYH7). A 700-kb cosmid contig containing the D14S990 and the myosin genes and a long-range restriction map covering the region between D14S990 and the MYH6 and MYH7 gene cluster were established. A detailed EcoRI restriction map of the cosmid contig was determined, and five putative CpG islands were identified. Based on these data, the four loci were mapped within an approximately 600-kb region with the following centromere to telomere order: D14S283, D14S990, MYH6, and MYH7. The YAC and cosmid contigs will facilitate the identification of genes lying within the OPMD candidate interval.
Center for Research in Neuroscience, McGill University, Montreal General Hospital, 1650 Cedar Avenue, Montreal, Quebec, H3G 1A4, Canada.
Y G
YG
Xie
D
D
Rochefort
B
B
Brais
H
H
Howard
F Y
FY
Han
L P
LP
Gou
P
P
Maciel
B T
BT
The
C
C
Larsson
G A
GA
Rouleau
D016386:Chromosome Walking; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D020451:Contig Mapping; D016521:Electrophoresis, Gel, Pulsed-Field; D005796:Genes / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D009136:Muscular Dystrophies / Q000235:genetics*; D009801:Oculomotor Muscles; D010609:Pharyngeal Muscles; D015183:Restriction Mapping
D005819:Genetic Markers