Patch-clamp, ion-sensing, and glutamate-sensing techniques to study glutamate transport in isolated retinal glial cells.
We have described how a combination of electrical, ion-sensing, and glutamate-sensing techniques has advanced our understanding of glutamate uptake into isolated salamander retinal glial cells. The next steps in understanding glutamate transport will inevitably depend strongly on molecular biological methods, as described elsewhere in this book, but will also require more detailed study of transporters in their normal environment, perhaps by using patch-clamping or imaging techniques to study cells in situ.
Department of Physiology, University College London, England.
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Billups
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Szatkowski
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Rossi
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Attwell
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D027322:Amino Acid Transport System X-AG; D000818:Animals; D001692:Biological Transport; D002531:Cerebellum / Q000502:physiology*; D004558:Electric Stimulation; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018698:Glutamic Acid / Q000031:analogs & derivatives / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009457:Neuroglia / Q000378:metabolism / Q000502:physiology*; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000502:physiology*; D051381:Rats; D012160:Retina / Q000166:cytology*; D014562:Caudata
D018528:ATP-Binding Cassette Transporters; D027322:Amino Acid Transport System X-AG; D005452:Fluoresceins; D005456:Fluorescent Dyes; D018698:Glutamic Acid; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein