Enhanced electron-density envelopes by extended solvent definition.
Extended delineation of water molecules, monitored using Rfree values, afforded considerable improvement in quality of electron-density maps for structure determination of mammalian class I and E. coli class II aldolases. Augmented solvent definition results in an additional decrease in Rfree values of 3-4% and is reflected in significantly enhanced electron-density envelopes enabling tracing of amino-acid sequences through regions of otherwise discontinuous or weak electron density.
Département de biochemie, Université de Montréal, CP 6128 Station Centre Ville, Montréal, Canada H3C 3J7. nick@bch.umontreal.ca
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Sygush
D004583:Electrons; D004926:Escherichia coli / Q000201:enzymology*; D005634:Fructose-Bisphosphate Aldolase / Q000737:chemistry*; D008958:Models, Molecular; D012997:Solvents
D012997:Solvents; D005634:Fructose-Bisphosphate Aldolase