On-line flow displacement immunoassay for fatty acid-binding protein.
In standard displacement flow immunoassays the analyte in the sample creates an active dissociation of labelled antigens (or antigen homologues) from an antigen binding site of an immobilized antibody, after which the labelled substance is measured downstream. Such systems have been described for molecules up to 1 kDa. In this study, we demonstrate displacement in a flow system for the detection of a small protein, cytoplasmic heart-type fatty acid-binding protein (15 kDa), a plasma marker for myocardial injury. The displacement system uses an inverse set-up: enzyme labelled monoclonal antibodies are associated to immobilized antigen, and are displaced by analyte in the sample. The system permits detection of both physiological (2-12 microg l(-1)) and pathological concentrations (12-2000 microg l(-1)) of fatty acid-binding protein in an on-line flow system.
Kaptein
W A
WA
Department of Chemistry, Hong Kong University of Science and Technology, Kowloon, China.
Korf
J
J
Cheng
S
S
Yang
M
M
Glatz
J F
JF
Renneberg
R
R
D000818:Animals; D015415:Biomarkers; D002352:Carrier Proteins / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D007118:Immunoassay / Q000379:methods*; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D018996:Myelin P2 Protein / Q000032:analysis*; D009203:Myocardial Infarction / Q000097:blood / Q000175:diagnosis; D009206:Myocardium / Q000737:chemistry; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D011994:Recombinant Proteins / Q000032:analysis; D012212:Rheology; D013045:Species Specificity
D015415:Biomarkers; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011994:Recombinant Proteins