The use of Escherichia coli bearing a phoN gene for the removal of uranium and nickel from aqueous flows.
A Citrobacter sp. originally isolated from metal-polluted soil accumulates heavy metals via metalphosphate deposition utilizing inorganic phosphate liberated via PhoN phosphatase activity. Further strain development was limited by the non-transformability of this environmental isolate. Recombinant Escherichia coli DH5 alpha bearing cloned phoN or the related phoC acquired metal-accumulating ability, which was compared with that of the Citrobacter sp. with respect to removal of uranyl ion (UO2(2+)) from dilute aqueous flows and its deposition in the form of polycrystalline hydrogen uranyl phosphate (HUO2PO4). Subsequently, HUO2PO4-laden cells removed Ni2+ from dilute aqueous flows via intercalation of Ni2+ into the HUO2PO4 lattice. Despite comparable acid phosphatase activity in all three strains, the E. coli DH5 alpha (phoN) construct was superior to Citrobacter N14 in both uranyl and nickel accumulation, while the E. coli DH5 alpha (phoC) construct was greatly inferior in both respects. Expression of phosphatase activity alone is not the only factor that permits efficient and prolonged metal phosphate accumulation, and the data highlight possible differences in the PhoN and PhoC phosphatases, which are otherwise considered to be related in many respects.
Basnakova
G
G
School of Biological Sciences, University of Birmingham, UK.
Stephens
E R
ER
Thaller
M C
MC
Rossolini
G M
GM
Macaskie
L E
LE
D001673:Biodegradation, Environmental; D002954:Citrobacter / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005798:Genes, Bacterial; D009532:Nickel / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants; D014501:Uranium / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; D014501:Uranium; D009532:Nickel; D010744:Phosphoric Monoester Hydrolases