Isolation and structure of pompilidotoxins, novel peptide neurotoxins in solitary wasp venoms.
Novel peptide neurotoxins, alpha- and beta-pompilidotoxins (alpha- and beta-PMTXs), were purified from the venoms of the solitary wasps Anoplius samariensis and Batozonellus maculifrons. Their structures were analyzed mostly by MALDI-TOF-MS, which were corroborated by solid-phase synthesis. alpha-PMTX, with 13 amino acid residues and the sequence of Arg-Ile-Lys-Ile-Gly-Leu-Phe-Gln-Asp-Leu-Ser-Lys-Leu-NH2, greatly potentiates synaptic transmission of lobster leg muscle by the presynaptic mechanisms. beta-PMTX, in which the lysine residue at 12 position of alpha-PMTX was replaced with arginine, was more potent than alpha-PMTX.
Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa, 199-0195, Japan.
K
K
Konno
M
M
Hisada
Y
Y
Itagaki
H
H
Naoki
N
N
Kawai
A
A
Miwa
T
T
Yasuhara
H
H
Takayama
D000818:Animals; D019476:Insect Proteins; D008121:Nephropidae; D009469:Neuromuscular Junction / Q000187:drug effects; D009498:Neurotoxins / Q000302:isolation & purification* / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects; D014862:Wasp Venoms; D014863:Wasps
D019476:Insect Proteins; D009498:Neurotoxins; D014862:Wasp Venoms; C115328:pompilidotoxin alpha; C115329:pompilidotoxin beta