[Standardization of an indirect ultramicro ELISA for the determination of total antibodies against the measles virus].
An indirect ultramicro ELISA system (UME) for the determination of antibodies to measles virus by the cuban technology of Ultramicro Analytical Systems (SUMA) was developed. A number of 448 serum samples from the National System of Seroepidemiological Surveillance of Measles were simultaneously processed by the Ultramicro ELISA system and by the haemagglutination inhibition technique. Results were compared in both assays. Afterwards; 120 serum pairs obtained from the same source were studies and processed in a similar way. Results evidenced the usefulness of such immunoenzymatic system for the serological diagnosis and the positive validation of replacing conventional techniques by this novel technology.
Instituto de Medicina Tropical Pedro Kouri.
M
M
Soler Nodarse
D R
DR
Domínguez
S
S
Vázquez Ramudo
J
J
Laferté Serrano
M G
MG
Guzmán Tirado
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008459:Measles virus / Q000276:immunology* / Q000502:physiology; D012680:Sensitivity and Specificity; D014709:Vero Cells; D014779:Virus Replication
D000914:Antibodies, Viral
Estandarización de un sistema ultramicroELISA indirecto para la determinación de anticuerpos totales al virus del sarampión.