Terminology and nomenclature for standardization in quantitative fluorescence cytometry.
Terminology in any field is a complex mix of established conventions, accepted usages, disputed terms, and occasional misnomers. The terminology that has evolved for quantitative fluorescence cytometry (QFCM) is especially multifarious, in part because QFCM encompasses a range from subjective visual assessments to objective photon counts. Thus, while descriptive terms such as "dim" and "bright" are still quite useful, quantitative terms such as "binding capacity" should be used with collective understanding of their exact meanings. This article reviews current usage and proposes definitions that, with refinement from suppliers and users of QFCM technology, can provide the required clarity.
National Diabetes Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, Georgia, USA.
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Henderson
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Marti
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Gaigalas
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Hannon
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Vogt
D002138:Calibration; D005434:Flow Cytometry / Q000379:methods / Q000592:standards*; D005456:Fluorescent Dyes; D012015:Reference Standards; D009626:Terminology as Topic
D005456:Fluorescent Dyes