Co-localization of 5-HT1B- and 5-HT1D receptor mRNA in serotonergic cell bodies in guinea pig dorsal raphé nucleus: a double labeling in situ hybridization histochemistry study.
In order to provide further details on the cellular localization of 5-HT1B- and 5-HT1D receptor mRNA in the dorsal raphé nucleus, we performed, in the same sections of guinea-pig dorsal raphé nucleus, double labeling in situ hybridization histochemistry for: (1) 5-HT1B receptor mRNA and 5-HT1D receptor mRNA, (2) 5-HT1B receptor mRNA and 5-HT transporter (5-HTT) mRNA as marker for serotonergic neurons and (3) 5-HT1D receptor mRNA and 5-HTT mRNA. The 5-HT1B receptor mRNA was present in all cells containing 5-HT1D receptor mRNA. Similarly, both 5-HT1B- and 5-HT1D receptor mRNA was present in all 5-HTT mRNA positive cells. The present study demonstrates that 5-HT1B- and 5-HT1D receptor mRNA is co-localized in serotonergic cell bodies of the guinea pig dorsal raphé nucleus.
Bonaventure
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P
Department of Biochemical Pharmacology, Janssen Research Foundation, Beerse, Belgium. pbonaven@janbe.jnj.com
Langlois
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X
Leysen
J E
JE
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D006168:Guinea Pigs; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D009474:Neurons / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D011903:Raphe Nuclei / Q000166:cytology / Q000378:metabolism*; D011985:Receptors, Serotonin / Q000235:genetics*; D012701:Serotonin / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins; D014018:Tissue Distribution
D002352:Carrier Proteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D011985:Receptors, Serotonin; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin