Intestinal lactase-phlorizin hydrolase (LPH): the two catalytic sites; the role of the pancreas in pro-LPH maturation.
Brush border lactase-phlorizin hydrolase carries two catalytic sites. In the human enzyme lactase comprises Glu-1749, phlorizin hydrolase Glu-1273. The proteolytic processing of pro-lactase-phlorizin hydrolase by (rat) enterocytes stops two amino acid residues short of the N-terminus of 'mature' final, brush border lactase-phlorizin hydrolase. Only these two amino acid residues are removed by luminal pancreatic protease(s), probably trypsin.
Zecca
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Department of Biochemistry, Swiss Federal Institute of Technology, ETH Zentrum, Zurich.
Mesonero
J E
JE
Stutz
A
A
Poirée
J C
JC
Giudicelli
J
J
Cursio
R
R
Gloor
S M
SM
Semenza
G
G
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D004789:Enzyme Activation; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D010694:Lactase-Phlorizin Hydrolase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*
D010694:Lactase-Phlorizin Hydrolase