Characterization and distribution of HLA-B*5002 in a Spanish population sample.
HLA-B45, in contrast to B44, does not show molecular polymorphism. We have found a group of Caucasian Spanish individuals, serologically typed as B45, showing an unexpected HLA-B12 PCR-SSO subtyping pattern. Complete coding region sequencing and B45 subtyping by PCR-SSO demonstrated that the B45 serologic specificity is constituted by two molecular alleles: B*4501 and B*5002. B*5002 is recognized by polyclonal and monoclonal allosera against B12 and B45, whereas it is not detected by B21, B49, or B50 reagents, providing a new example of poor correlation between serology and structure. B*5002 explains an important subset (18%) of the B45-positive individuals of the Spanish population studied, and almost half are included in a very infrequent haplotypic association, Cw6-B*5002-DRB1*0406-DQA1*03-DQB1*0402.
Histocompatibility, Regional Transfusion Centre, Madrid, Spain. ctm@bitmailer.net
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Balas
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Santos
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García-Sánchez
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Lillo
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Merino
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Vicario
D000595:Amino Acid Sequence; D003429:Cross Reactions; D015342:DNA Probes; D015870:Gene Expression / Q000276:immunology; D005787:Gene Frequency; D015235:HLA-B Antigens / Q000235:genetics* / Q000276:immunology*; D059948:HLA-B44 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013030:Spain; D014181:Transplantation Immunology / Q000235:genetics; D044465:White People / Q000235:genetics*
D015342:DNA Probes; D015235:HLA-B Antigens; D059948:HLA-B44 Antigen; C077377:HLA-B45 antigen