Interleukin-10 promoter polymorphisms in rheumatoid arthritis and Felty's syndrome.
OBJECTIVE
To examine whether promoter polymorphisms associated with variation in interleukin-10 (IL-10) production are relevant to the development of rheumatoid arthritis (RA) or Felty's syndrome (FS).


METHODS
DNA was obtained from 44 FS patients, 117 RA patients and 295 controls. The promoter region between -533 and - 1120 was amplified by polymerase chain reaction, and polymorphisms detected by restriction enzyme digest or sequence-specific oligonucleotide probing.


RESULTS
We found no significant difference in allele or haplotype frequencies between the groups.


CONCLUSIONS
There is no association between FS or RA and these recently identified IL-10 promoter polymorphisms. Other genetic or environmental factors could explain the alterations in IL-10 levels seen in these conditions.
Department of Rheumatology, Guy's, King's and St Thomas' School of Medicine, Guy's Hospital, London.
G
G
Coakley
C C
CC
Mok
A H
AH
Hajeer
W E
WE
Ollier
D
D
Turner
P J
PJ
Sinnott
I V
IV
Hutchinson
G S
GS
Panayi
J S
JS
Lanchbury
D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology; D016022:Case-Control Studies; D005258:Felty Syndrome / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics*
D016753:Interleukin-10