Do specific or nonspecific interactions with proteins underlie inhalational anesthetic action?
To determine whether specific or nonspecific interactions between inhaled anesthetics and proteins are more likely to underlie anesthetic actions, analysis of hydrogen/tritium exchange was used to measure effects on the stability of two model proteins that had been previously shown to bind anesthetics specifically (bovine serum albumin) or only nonspecifically (myoglobin). The data indicated that stabilization of albumin correlated with the potencies of a wide range of anesthetic compounds significantly better than did destabilization of myoglobin. In addition, sensitivity to nonanesthetics, isoflurane stereoselectivity, and temperature and pressure effects all influenced the stabilization of bovine serum albumin, but not the destabilization of myoglobin, in a manner strongly supporting the premise that specific binding interactions with protein targets underlie anesthetic action. These observations significantly increase the likelihood that such interactions can be found and optimized.
Departments of Anesthesia and Physiology, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104-4283, USA. reckenho@mail.med.upenn.edu
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D018685:Anesthetics, Inhalation / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D006874:Hydrostatic Pressure; D009211:Myoglobin / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013816:Thermodynamics
D018685:Anesthetics, Inhalation; D009211:Myoglobin; D012710:Serum Albumin, Bovine