Monoclonal antibodies directed against lipopolysaccharide of Legionella pneumophila serogroup 1.
The monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG-secreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.
Baranov
A M
AM
State Research Center for Applied Microbiology, Moscow Region, Russia.
Vetchinin
S S
SS
Baranova
E V
EV
Biketov
S F
SF
Svetoch
E A
EA
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D016952:Legionella pneumophila / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides