Use of P-glycoprotein gene probes to investigate anthelmintic resistance in Haemonchus contortus and comparison with Onchocerca volvulus.
A P-glycoprotein gene probe from the sheep parasitic nematode Haemonchus contortus was developed and used to analyse restriction fragment length polymorphisms between susceptible isolates and isolates resistant to either benzimidazole; levamisole and benzimidazole; or benzimidazole, ivermectin and closantel. No polymorphism could be correlated with any of the different resistances. A P-glycoprotein gene probe was also isolated from the human nematode parasite Onchocerca volvulus and an Onchocerca-specific PCR was developed.
Kwa
M S
MS
Department of Parasitology, University of Utrecht, The Netherlands.
Okoli
M N
MN
Schulz-Key
H
H
Okongkwo
P O
PO
Roos
M H
MH
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D015139:Blotting, Southern; D003001:Cloning, Molecular; D015342:DNA Probes / Q000235:genetics*; D018509:DNA, Helminth; D018432:Drug Resistance, Multiple / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005369:Filaricides; D017238:Genes, Helminth / Q000235:genetics; D006189:Haemonchus / Q000187:drug effects* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D017181:Onchocerca volvulus / Q000187:drug effects* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D012756:Sheep; D013045:Species Specificity
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D015342:DNA Probes; D018509:DNA, Helminth; D005369:Filaricides