Primary intestinal epithelial cells can be infected with laboratory-adapted strain HIV type 1 NDK but not with clinical primary isolates.
Infectivities of HIV-1 primary isolates and laboratory-adapted strains were compared in primary fetal enterocytes and the colonic epithelial cell line HT29. Infection by two laboratory strains, HIV-1 NDK and HIV-1 NDK(A4), which were adapted on CEM and HT29 cells, respectively, produced significant amounts of virus in both target cell systems. Intestinal cells were resistant to infection with HIV-1 primary isolates regardless of their genetic subtype or SI/NSI phenotype. Biological properties of analyzed viruses rather than differences in cultivation system seem to be responsible for differences between these in vitro and ex vivo results.
Chenine
A L
AL
INSERM U322, Unité de Recherches sur les Rétrovirus et maladies associées, Marseille, France.
Matouskova
E
E
Sanchez
G
G
Reischig
J
J
Pavlikova
L
L
LeContel
C
C
Chermann
J C
JC
Hirsch
I
I
D002478:Cells, Cultured; D004848:Epithelium / Q000821:virology; D005260:Female; D005333:Fetus; D015658:HIV Infections / Q000821:virology*; D015497:HIV-1 / Q000302:isolation & purification* / Q000472:pathogenicity* / Q000502:physiology; D019073:HT29 Cells; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000166:cytology* / Q000821:virology*; D007633:Keratins / Q000032:analysis; D010641:Phenotype; D011247:Pregnancy
D007633:Keratins