The efficacy of trypsin: chymotrypsin preparation in the reduction of oxidative damage during burn injury.
This study was mainly aimed to investigate the efficacy of trypsin:chymotrypsin to elicit anti-oxidant properties. In our earlier studies it was observed that the enzyme preparation exhibited an anti-inflammatory action as there was a remarkable reduction in oedema formation and tissue destruction. This led to further study on the amount of lipid peroxidation products formed and the levels of enzymatic and non-enzymatic anti-oxidants and relative trace element contents of copper, selenium, iron and zinc during administration of the enzyme preparation. Decreased formation of lipid peroxidation products was observed in treated group in comparison with the untreated group. Higher levels of enzymatic anti-oxidants mainly super oxide dismutase, catalase, glutathione peroxidase and glutathione-s-transferase and non-enzymatic antioxidant namely ceruloplasmin persisted for a longer period of time in the treated group than in the untreated group. No statistical significance was observed in non-enzymatic antioxidants viz. ascorbic acid and tocopherol levels in both the groups. Increased serum copper and selenium levels in the treated group could be related to higher levels of the ceruloplasmin and glutathione peroxidase observed in the treated group. The above studies support the finding that treatment with the enzyme preparation reduced tissue destruction leading to decreased formation of free radicals and subsequent effective scavenging of free radicals by the higher levels of enzymatic and non-enzymatic anti-oxidants.
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, India.
B
B
Latha
M
M
Ramakrishnan
V
V
Jayaraman
M
M
Babu
D000328:Adult; D002056:Burns / Q000188:drug therapy* / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D002918:Chymotrypsin / Q000627:therapeutic use*; D005260:Female; D005500:Follow-Up Studies; D005609:Free Radicals / Q000037:antagonists & inhibitors; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D014131:Trace Elements / Q000097:blood; D016896:Treatment Outcome; D014357:Trypsin / Q000627:therapeutic use*; D014815:Vitamins / Q000097:blood
D005609:Free Radicals; D014131:Trace Elements; D014815:Vitamins; D005979:Glutathione Peroxidase; D002570:Ceruloplasmin; D002918:Chymotrypsin; D014357:Trypsin