[Characterization of aldehyde dehydrogenase gene fragment from mung bean Vigna radiata using the polymerase chain reaction].
Two degenerate oligonucleotide sequence primers and polymerase chain reactions on total DNA have been utilized to clone on 651--bp gene fragment coding the central part of amino acid sequence of an earlier unknown aldehyde dehydrogenase (ALDH) from mung bean. The deduced partial amino acid sequence for this aldehyde dehydrogenase shows about 65% sequence identity to ALDHs of Vibrio cholerae Rhodococcus sp., Alcaligenes eutrophus and about 45% sequence identity to mammalian ALDHs 1 and 2, ALDHs of Aspergillus niger and A, nidulans, the betain aldehyde dehydrogenase from spinach. Alignment of the mung bean aldehyde dehydrogenase partial amino acid sequence with the sequence of 16 NAD(P)(+)-dependent aldehyde dehydrogenases has demonstrated that all strictly conserved amino acid residues and all three conservative regions are identical.
Institute for Biological Problems of Cryolithozone, Siberian Branch, Russian Academy of Sciences, Yakutsk.
A G
AG
Ponomarev
V V
VV
Bubiakina
T D
TD
Tatarinova
S M
SM
Zelenin
D000444:Aldehyde Dehydrogenase / Q000235:genetics*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D007887:Fabaceae / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal; D016415:Sequence Alignment; D017421:Sequence Analysis
D010940:Plant Proteins; D000444:Aldehyde Dehydrogenase
Kharakteristika fragmenta gena al'degiddegidrogenazy mash Vigna radiata s pomoshch'iu metoda polimeraznoĭ tsepnoĭ reaktsii.