Effect of macrophage colony-stimulating factor on mouse NK 1.1+ cell activity in vivo.
The effect of recombinant human macrophage colony-stimulating factor (rhM-CSF) on NK 1.1+ cell activity in vivo and in vitro was studied. An intravenous injection of rhM-CSF increased the numbers of NK 1.1+ cells in mouse spleen and blood and augmented the clearance of Yac-1 cells in vivo. Using a magnetic cell sorter (MACS), we purified NK 1.1+ cells from vehicle-injected and rhM-CSF-injected mouse spleen cells. More than 95% of the collected cells were NK 1.1 antigen-positive. NK 1.1+ cells purified from rhM-CSF-injected mouse spleen cells exhibited (a) higher cytotoxic activity against Yac-1 cells, (b) higher proliferative responsiveness to interleukin (IL)-2 and (c) a greater production of interferon (IFN)-gamma in response to IL-2 and IL-12 compared to cells purified from vehicle-injected mouse spleen cells in vitro. These results suggest that the administration of rhM-CSF increases NK 1.1+ cell numbers and activates the cells in vivo.
Biochemical Research Laboratory, Morinaga Milk Industry Co. Ltd, Higashihara Zama City, Kanagawa pref., Japan.
T
T
Sakurai
N
N
Wakimoto
M
M
Yamada
S
S
Shimamura
K
K
Motoyoshi
D000818:Animals; D002452:Cell Count / Q000187:drug effects; D003602:Cytotoxicity, Immunologic; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000494:pharmacology; D007694:Killer Cells, Natural / Q000187:drug effects* / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D016173:Macrophage Colony-Stimulating Factor / Q000097:blood / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology
D007376:Interleukin-2; D016173:Macrophage Colony-Stimulating Factor; D007371:Interferon-gamma