Preliminary X-ray analysis of a new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis.
A new crystal form of the vanadium-dependent bromoperoxidase from Corallina officinalis has been obtained. The crystals exhibit a 'teardrop' morphology and are grown from 2 M ammonium dihydrogen phosphate pH and diffract to beyond 1.7 A resolution. They are in tetragonal space group P4222 with unit-cell dimensions of a = b = 201.9, c = 178.19 A, alpha = beta = gamma = 90 degrees. A 2.3 A resolution native data set has been collected at the Hamburg Synchrotron. A mercury derivative data set has also been collected, and the heavy-atom positions have been determined. The self-rotation function and the positions of the heavy atoms are consistent with the molecule being a dodecamer with local 23 symmetry.
Departments of Chemistry and Biological Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, England.
A A
AA
Brindley
A R
AR
Dalby
M N
MN
Isupov
J A
JA
Littlechild
D018360:Crystallography, X-Ray; D008970:Molecular Weight; D010544:Peroxidases / Q000737:chemistry*; D012624:Seaweed / Q000201:enzymology*; D017356:Synchrotrons
D010544:Peroxidases; C026195:bromide peroxidase