Effects of dentin bonding agents on macrophage mitochondrial activity.
Dentin bonding agents (DBA) have been considered for use as root-end fillings. Previous studies have documented the release of DBA components in vivo and in vitro, but the biological implications are not clear. The macrophage is important in wound healing, and likely to be important in any inflammatory response. Therefore, this study determined the concentrations of the components of DBAs that suppress the mitochondrial activity of human macrophages in vitro. THP-1 macrophages were cultured in the presence of four DBA components (2-hydroxyethyl methacrylate (HEMA), 4-methacryloxyethyl trimellitate anhydride (4-META), bisphenol-glycidylmethacrylate (Bis-GMA), and urethane dimethacrylate (UDMA)) at various concentrations and for varying durations. Residual effects were also measured after the resins were removed. Controls received only the vehicle solution, ethanol or water. THP-1 mitochondrial activity was estimated using the MTT assay, and the 50% toxicity concentrations (TC50) were determined graphically. Resin components suppressed the mitochondrial activity of macrophages at different concentrations (TC50 values for HEMA (10,000 mumol/L), 4-META (3,800 mumol/L), Bis-GMA (130 mumol/L), and UDMA (110 mumol/L) at 24 h, and the effect was time-dependent. Residual effects were observed for all resins.
Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia, Augusta 30912-1260, USA.
D R
DR
Rakich
J C
JC
Wataha
C A
CA
Lefebvre
R N
RN
Weller
D000180:Acrylic Resins / Q000494:pharmacology; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D002478:Cells, Cultured; D017220:Dentin-Bonding Agents / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D008689:Methacrylates / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D011140:Polyurethanes / Q000494:pharmacology; D012184:Retrograde Obturation; D012387:Root Canal Filling Materials / Q000494:pharmacology; D013385:Succinate Dehydrogenase / Q000378:metabolism*; D013997:Time Factors
D000180:Acrylic Resins; D017220:Dentin-Bonding Agents; D008689:Methacrylates; D011140:Polyurethanes; D012387:Root Canal Filling Materials; C029824:urethane dimethacrylate luting resin; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; C031172:4-META resin; D013385:Succinate Dehydrogenase