Determination of acetaldehyde in rat blood by the use of rat liver aldehyde dehydrogenase.
A method has been developed for the determination of low concentrations of acetaldehyde in rat blood. The method involves extraction of blood in perchloric acid followed by a fluorimetric determination of acetaldehyde in neutralized extracts by the use of a low K(m) aldehyde dehydrogenase isolated from rat liver mitochondria. Acetaldehyde concentrations down to 2 to 3 microM could be detected in blood samples of 0.1 ml containing high concentrations of ethanol (10-40 mM). Due to its simplicity, sensitivity, and the use of a low-cost fluorimeter, this enzymatic method should be a valuable complement to gas chromatographic methods for acetaldehyde determination.
Tottmar
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O
Alcohol Research Group of the Swedish Medical Research Council, Institute of Zoophysiology, University of Uppsala, Sweden.
Marchner
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H
Pettersson
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H
D000079:Acetaldehyde / Q000097:blood*; D000444:Aldehyde Dehydrogenase; D000818:Animals; D000431:Ethanol / Q000097:blood; D008930:Mitochondria, Liver / Q000201:enzymology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000431:Ethanol; D000444:Aldehyde Dehydrogenase; D000079:Acetaldehyde