Stimulation of macrophages by Bacillus firmus: production of nitric oxide and cytokines.
Immunostimulatory properties of gram-positive Bacillus firmus were investigated under in vitro conditions using murine peritoneal macrophages. B. firmus stimulated in a concentration and time dependent manner the secretion of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10), but it had no influence upon interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) production. It also substantially augmented production of nitric oxide (NO) induced by exogenous IFN-gamma. Inhibitory experiments using neutralizing antibodies against TNF-alpha and/or IL-10 have demonstrated that these cytokines are responsible for triggering the underlying mechanism(s) leading to enhanced NO production. The cytokine-stimulatory and NO-costimulatory properties could participate in the antiinfectious and anticancer effects of B. firmus, detected previously in the in vivo experiments.
Zidek
Z
Z
Institute of Pharmacology, Academy of Sciences of the Czech Republic, Prague.
Tucková
L
L
Mára
M
M
Barot-Ciorbaru
R
R
Prokesová
L
L
Tlaskalová-Hogenová
H
H
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D001407:Bacillus / Q000276:immunology*; D005260:Female; D007371:Interferon-gamma / Q000032:analysis / Q000494:pharmacology; D016753:Interleukin-10 / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*; D007376:Interleukin-2 / Q000032:analysis; D008070:Lipopolysaccharides / Q000494:pharmacology; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009615:Nocardia / Q000276:immunology; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007376:Interleukin-2; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D009569:Nitric Oxide; D007371:Interferon-gamma; D019001:Nitric Oxide Synthase