Potato virus A detection by reverse transcription-polymerase chain reaction.
Simple and reliable procedure for sample preparation and reverse transcription-polymerase chain reaction (RT-PCR) detection of potato virus A (PVA) is described. PVA-specific primers used in the RT-PCR defined a target sequence of 321 bp and did not produce amplification product(s) with potato virus Y.
Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
N
N
Cerovská
K
K
Petrzik
T
T
Moravec
I
I
Mráz
D015139:Blotting, Southern; D004797:Enzyme-Linked Immunosorbent Assay; D016133:Polymerase Chain Reaction / Q000379:methods*; D017800:Potyvirus / Q000302:isolation & purification*; D011198:Solanum tuberosum / Q000821:virology