Early glomerular effects of contrast media in rats: evaluation with a simple method.
The aim of this study was to evaluate the early effects of high and low-osmolar contrast media on glomerular function in rats by using a new method based on the measurement of the urinary excretion of 99mTc-DTPA. Thirty-six Sprague-Dawley male rats were examined: nine rats were injected with diatrizoate (ionic high-osmolar contrast medium), nine rats with iohexol (nonionic low-osmolar contrast medium), and nine rats with saline as controls. The urinary excretion of 99mTc-DTPA in the first minutes after i.v. injection was assumed as an index of glomerular filtration rate. A lower urinary excretion of 99mTc-DTPA was found in rats treated with contrast media in comparison with control rats. This effect was more evident after diatrizoate but was statistically significant also after iohexol. In conclusion, a reduction in the glomerular filtration rate probably occurs in the first few minutes after contrast media administration. The measurement of urinary excretion of 99mTc-DTPA could be a simple method to detect acute glomerular effects due to contrast media or to other drugs.
Unitá di Nefrologia, University of Pisa, Italy.
C
C
Donadio
G
G
Tramonti
A
A
Lucchesi
I
I
Auner
C
C
Bianchi
D000818:Animals; D003287:Contrast Media / Q000633:toxicity*; D003973:Diatrizoate / Q000633:toxicity*; D004195:Disease Models, Animal; D005919:Glomerular Filtration Rate / Q000187:drug effects; D007275:Injections, Intravenous; D007472:Iohexol / Q000633:toxicity*; D007479:Iopamidol / Q000633:toxicity*; D007678:Kidney Glomerulus / Q000187:drug effects*; D008297:Male; D009994:Osmolar Concentration; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D016284:Technetium Tc 99m Pentetate / Q000097:blood / Q000652:urine*; D013997:Time Factors
D003287:Contrast Media; D003973:Diatrizoate; D007472:Iohexol; D007479:Iopamidol; D016284:Technetium Tc 99m Pentetate