Diagnosis of porcine cysticercosis: a comparative study of serological tests for detection of circulating antibody and viable parasites.
Epidemiological studies of porcine cysticercosis require identification of pigs harbouring viable Taenia solium cysticerci and estimates of the degree of exposure to the parasite in the pig population destined for human consumption. Identification of infected pigs with viable larvae is achieved through detection of their secretory products. However, detectable levels of circulating antibody may also be present in the absence of viable larvae. In this study, both types of tests have been evaluated in groups of pigs experimentally infected with T. solium. Detection of viable cysticerci was achieved using a monoclonal antibody-based (HP10) antigen capture assay. HP10 epitope-bearing antigens have now been demonstrated in T. solium and T. crassiceps cyst fluid and excretion/secretions. Serum antibodies were measured in ELISA assays using two parasite preparations as antigens; T. solium cyst fluid and T. crassiceps cyst fluid antigens bearing the HP10 epitope. Low-background values were obtained with sera from non-infected animals in all the assays used. In heavily infected pigs, both antigens and antibodies were detected at least 29 days and up to 200 days post-infection (pi), while in lightly infected pigs antigen and antibodies were first observed between 61-97 days pi. Thus, the levels of the serum antigen and antibody varied with the intensity of the infection.
Sciutto
E
E
Instituto de Investigaciones Biomédicas, UNAM D.F., Mexico City, Mexico. edda@servidor.unam.mx
Hernández
M
M
García
G
G
de Aluja
A S
AS
Villalobos
A N
AN
Rodarte
L F
LF
Parkhouse
M
M
Harrison
L
L
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth / Q000032:analysis / Q000097:blood; D015153:Blotting, Western / Q000662:veterinary; D003551:Cysticercosis / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D003552:Cysticercus / Q000276:immunology* / Q000502:physiology; D004306:Dose-Response Relationship, Immunologic; D004586:Electrophoresis / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D007411:Intestinal Diseases, Parasitic / Q000175:diagnosis / Q000517:prevention & control / Q000662:veterinary*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology / Q000517:prevention & control
D000909:Antibodies, Helminth; D000911:Antibodies, Monoclonal; D000947:Antigens, Helminth