P-Element insertion at the polyhomeotic gene leads to formation of a novel chimeric protein that negatively regulates yellow gene expression in P-element-induced alleles of Drosophila melanogaster.
Polyhomeotic is a member of the Polycomb group (Pc-G) of homeotic repressors. The proteins encoded by the Pc-G genes form repressive complexes on the polycomb group response element sites. The phP1 mutation was induced by insertion of a 1.2-kb P element into the 5' transcribed nontranslated region of the proximal polyhomeotic gene. The phP1 allele confers no mutant phenotype, but represses transcription of P-element-induced alleles at the yellow locus. The phP1 allele encodes a chimeric P-PH protein, consisting of the DNA-binding domain of the P element and the PH protein lacking 12 amino-terminal amino acids. The P-PH, Polycomb (PC), and Posterior sex combs (PSC) proteins were immunohistochemically detected on polytene chromosomes in the regions of P-element insertions.
Belenkaya
T
T
Department of the Control of Genetic Processes, Russian Academy of Sciences, Moscow 117334, Russia.
Soldatov
A
A
Nabirochkina
E
E
Biryukova
I
I
Birjukova
I
I
Georgieva
S
S
Georgiev
P
P
D000818:Animals; D003433:Crosses, Genetic; D004251:DNA Transposable Elements / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics; D019476:Insect Proteins / Q000032:analysis / Q000235:genetics; D009154:Mutation / Q000235:genetics; D009698:Nucleoproteins / Q000032:analysis / Q000235:genetics*; D063150:Polycomb Repressive Complex 1; D012333:RNA, Messenger / Q000032:analysis; D011993:Recombinant Fusion Proteins / Q000032:analysis; D019895:Transposases / Q000235:genetics; D014960:X Chromosome / Q000737:chemistry
D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D019476:Insect Proteins; D009698:Nucleoproteins; C066770:Pc protein, Drosophila; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; C072820:ph-d protein, Drosophila; C050680:y protein, Drosophila; C071050:Psc protein, Drosophila; D063150:Polycomb Repressive Complex 1; D019895:Transposases