Monocyte-dependent stimulation of human T cells by silicon dioxide.
Mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation from randomly selected silicone breast implant recipients for testing. Restricted antibody to HLA-DR (28-33 kD) depleted the concanavalin A mitogenic response which was expected but failed to inhibit the proliferative response to silicon dioxide. Further testing with monoclonal antibodies to HLA-DP, -DQ, and a second -DR with specificity for the NS1 region of the MHC class II genome, all markedly inhibited proliferation of T cells despite otherwise adequate stimulation by concanavalin A or silicon dioxide. Monoclonal antibodies directed against B7-1 also inhibited proliferation of T cells following stimulation with concanavalin A or silicon dioxide. These results confirm the T-cell response to silicon dioxide is monocyte-dependent and not a superantigen as has been speculated.
Baptist Regional Laboratories, The University of Tennessee, Memphis, Tenn., USA. dsmalley@utmem1.utmem.edu
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Shanklin
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Hall
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D018427:Breast Implants / Q000009:adverse effects*; D002450:Cell Communication; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D006682:HLA-DP Antigens / Q000276:immunology; D006683:HLA-DQ Antigens / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D009000:Monocytes / Q000276:immunology*; D012822:Silicon Dioxide / Q000009:adverse effects* / Q000276:immunology; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology*
D000911:Antibodies, Monoclonal; D006682:HLA-DP Antigens; D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D003208:Concanavalin A; D012822:Silicon Dioxide