Crystallization and preliminary X-ray diffraction studies of a family 26 endo-beta-1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa.
Crystals of an endo-beta-1,4-mannanase (1,4-beta-D-mannohydrolase, E. C. 3.2.1.78) from Pseudomonas fluorescens sub species cellulosa have been grown by the hanging-drop technique at 291 K over a period of one to two weeks to maximal dimensions of 0.17 x 0.17 x 0.25 mm. These crystals belong to the space group R32 (or R3) with cell dimensions of a = b = 155.4 and c = 250.8 A (hexagonal setting) and contain three (six) molecules in the asymmetric unit. The crystals diffract to at least 3.2 A using a laboratory source and are suitable for structure determination.
Institute of Food Research, Earley Gate, Whiteknights Road, Reading, Berkshire RG6 6BZ, England.
M
M
Scott
R W
RW
Pickersgill
G P
GP
Hazlewood
D
D
Bolam
H J
HJ
Gilbert
G W
GW
Harris
D003460:Crystallization; D008361:Mannosidases / Q000737:chemistry*; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D012984:Software; D014961:X-Ray Diffraction
D008361:Mannosidases; C041867:endo-1,4-beta-D-mannanase