A new method for allantoin determination and its application in allantoin determination in Agrostemma githago L. Seed.
We have established several optimal conditions for qualitative and quantitative allantoin determination by applying Ehrlich's reagent. The limit of detection for allantoin determination amounts to 5 x 10(-6) mM. Allantoin is determined quantitatively by measuring the absorbance at 440 nm (from 300 to 1000 micrograms/ml). The color of the complex becomes stable by standing for 10 min at room temperature. We have used these conditions for allantoin determination in Agrostemma githago seed.
Vrbaski
M M
MM
Institute for Biological Research, Faculty of Science, Belgrade, Yugoslavia.
Grujić-Injac
B
B
Gajić
D
D
D000481:Allantoin / Q000032:analysis*; D001547:Benzaldehydes; D007202:Indicators and Reagents; D010944:Plants / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013053:Spectrophotometry
D001547:Benzaldehydes; D007202:Indicators and Reagents; D000481:Allantoin; C510241:p-dimethylaminobenzaldehyde