Increased mitochondrial DNA deletion in the brain of SAMP8, a mouse model for spontaneous oxidative stress brain.
Oxidative stress is considered to be closely correlated with degenerative brain abnormalities. In this study, the plausibility of a SAMP8 strain mouse showing memory deterioration and short life span as an oxidative stress brain model was evaluated. Mitochondrial DNA deletions were detected using polymerase chain reaction (PCR) as cumulative spontaneous oxidative stress. In the 4-8-week-old SAMP8 brain, multiple mitochondrial DNA (mtDNA) deletions were already found and the contents were significantly higher than those of SAMR1 or ddY controls. Enzyme activity studies indicated that electron transport was disturbed at the lower site of the chain and the electronegativity of the upper site might be increased, a cause of radical production and therefore oxidative stress.
Biomedical Imaging Research Center, Fukui Medical University, Japan. yfuji@pharm.kyoto-u.ac.jp
Y
Y
Fujibayashi
S
S
Yamamoto
A
A
Waki
J
J
Konishi
Y
Y
Yonekura
D000375:Aging / Q000502:physiology*; D000818:Animals; D001483:Base Sequence / Q000235:genetics; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D004272:DNA, Mitochondrial / Q000235:genetics*; D017353:Gene Deletion; D051379:Mice; D008817:Mice, Mutant Strains / Q000235:genetics; D018384:Oxidative Stress / Q000502:physiology*; D013997:Time Factors
D004272:DNA, Mitochondrial