Prepulse inhibition of the Tritonia escape swim.
Presenting a weak stimulus just before a strong, startle stimulus reduces the amplitude of the ensuing startle response in humans and other vertebrates. This phenomenon, termed "prepulse inhibition" (PPI), appears to function to reduce distraction while processing sensory input. To date, no detailed neural mechanism has been described for PPI. Here we demonstrate PPI in the marine mollusk Tritonia diomedea, which has a nervous system highly suitable for cellular analyses. We found that a 100 msec vibrotactile prepulse prevented the animal's escape swim response to a closely following 1 sec tail shock. This inhibition was highly transient, with a significant effect lasting just 2.5 sec. These findings indicate that the Tritonia escape swim response undergoes a form of PPI phenomenologically similar to that observed in vertebrates. Further tests showed that the vibrotactile stimulus had no inhibitory effect if applied after tail shock, while the animal was preparing to swim, but it acted to terminate swims once they were actively under way. As a first step toward a cellular analysis of PPI, we recorded from neurons of the swim circuit in a semi-intact preparation and found that the vibrotactile stimulus used in the behavioral experiments also prevented the tail shock-elicited swim motor program. These results represent the first explicit demonstration of PPI in an invertebrate and establish Tritonia as a model system for analyzing its physiological basis.
Department of Neurobiology and Anatomy, The University of Texas Medical School at Houston, Houston, Texas 77225, USA.
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Mongeluzi
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Hoppe
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Frost
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D004594:Electrophysiology; D004597:Electroshock; D004924:Escape Reaction / Q000502:physiology*; D008974:Mollusca / Q000502:physiology*; D009424:Nervous System Physiological Phenomena; D009433:Neural Inhibition / Q000502:physiology*; D013216:Reflex, Startle / Q000502:physiology; D013550:Swimming / Q000502:physiology*; D014110:Touch / Q000502:physiology