Preservation of plant cell ultrastructure during immunolocalization of virus particles.
Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus.
Department of Plant Sciences, University of Arizona, Tucson 85721, USA.
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Ghoshroy
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Citovsky
D017360:Arabidopsis / Q000648:ultrastructure / Q000821:virology; D001937:Brassica / Q000821:virology; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010944:Plants / Q000648:ultrastructure* / Q000821:virology*; D010947:Plants, Toxic; D016610:Tissue Embedding; D016707:Tissue Fixation; D014026:Nicotiana / Q000648:ultrastructure / Q000821:virology; D017898:Tobamovirus / Q000302:isolation & purification* / Q000648:ultrastructure