[Establishment of a subline of C6/36 adapted to growing in a serum-free medium and its usefulness for studies on the dengue virus].
Using the C6/36 clone of the Aedes albopictus cell line, a derived cell line was obtained capable of growing in a commercial serum-free medium facilitating this line to keep its morphologic characteristics and adhesion capacity. The growth speed rate reduction of the adapted cell line was compensated by its low maintenance cost. Its usefulness in the multiplication of dengue viruses 1 and 2 was tested; the reported line showed the same susceptibility for the 2 agents than the original cell line when the indirect fluorescence technique was applied.
Castillo Alvarez
A
A
Instituto de Medicina Tropical Pedro Kouri.
Morier Díaz
L
L
D002455:Cell Division; D002460:Cell Line; D016895:Culture Media, Serum-Free; D003716:Dengue Virus / Q000254:growth & development*; D019084:Fluorescent Antibody Technique, Indirect
D016895:Culture Media, Serum-Free
Obtención de una sublínea de C6/36 adaptada a crecer en medío libre de suero y su utilización para estudios sobre virus del dengue.