Prevention of cyclophosphamide-induced micronucleus formation in mouse bone marrow by indole-3-carbinol.
Indole-3-carbinol (I3C) is a glucobrassicin derivative isolated from cruciferous vegetables. In this study, the protective effect of 13C is reported against cyclophosphamide (CP)-induced micronuclei formation in mouse bone marrow cells. The three test doses, namely 500, 250 and 125 mg/kg body weight of 13C provided protection when given 48 hr prior to the single ip administration of cyclophosphamide (50 mg/kg). The efficacy of the test doses of 13C was also evaluated using a lower dose of CP (25 mg/kg body weight). A significant inhibition in micronuclei formation was noticed with 13C at 250 and 125 mg/kg body weight dose. 13C could not induce micronuclei formation at the test doses 500 and 250 mg/kg body weight. 13C, therefore seems to have a preventive potential against CP-induced micronuclei formation in Swiss mouse bone marrow cells.
Environmental Carcinogenesis Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
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Agrawal
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S
Kumar
D000818:Animals; D016588:Anticarcinogenic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000473:pathology; D002273:Carcinogens / Q000037:antagonists & inhibitors; D003520:Cyclophosphamide / Q000037:antagonists & inhibitors; D004305:Dose-Response Relationship, Drug; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects*; D015162:Micronucleus Tests
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D007211:Indoles; D003520:Cyclophosphamide; C016517:indole-3-carbinol