[Astrocytes and lentivirus infection in an experimental models of macaque infected with SIVmac251].
The present study demonstrates the susceptibility of astrocytes to infection with SIVmac251. Indeed, primary cultures of astrocytes derived from simian adult brains, can be infected in vitro with the SIVmac251. Results show that SIVmac251 establishes a persistent infection in primary astroglial cultures and that viral replication can be reactivated by TNF-alpha, GM-CSF, IFN-gamma. Viral proteins as Nef, Rev, Vpx and occasionally gp120/160 are evidenced by immunocytochemistry. In vivo SIVmac251 and/or HIV-2 infected astrocytes have been isolated from brains of macaques following ex vivo primary cultures. The whole of these results demonstrated that, in this model, SIV establishes a persistent state of infection of astrocytes, that viral replication can be reactivated by cytokines and moreover suggest strongly an in vivo infection of astrocytes in the brain of these infected macaques.
Service de Neurovirologie, Commissariat à l'énergie atomique, DSV/DRM, Fontenay-aux-Roses. g.guillemin@cfi.UNSW.EDU.au
G
G
Guillemin
J
J
Croitoru
F D
FD
Boussin
R
R
Le Grand
M
M
Franck-Duchenne
D
D
Dormont
D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000821:virology*; D001921:Brain / Q000166:cytology / Q000473:pathology / Q000821:virology*; D002478:Cells, Cultured; D016207:Cytokines / Q000494:pharmacology* / Q000502:physiology; D004279:DNA, Viral / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D015498:HIV-2 / Q000472:pathogenicity / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D008252:Macaca fascicularis; D008253:Macaca mulatta; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000494:pharmacology; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000503:physiopathology*; D015302:Simian Immunodeficiency Virus / Q000472:pathogenicity / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects / Q000502:physiology*
D016207:Cytokines; D004279:DNA, Viral; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
Astrocytes et infection lentivirale dans le modèle expérimental du macaque infecté par le SIVmac251.