Salicylate inhibits fimbriae mediated HEp-2 cell adherence of and haemagglutination by enteroaggregative Escherichia coli.
Enteroaggregative Escherichia coli (EAggEC) are associated with both acute and persistent diarrhoea in children. Bowel colonisation due to fimbrial adherence factors appears to play a major role in the disease process. In this study, we investigated the effect of sodium salicylate and 5-aminosalicylic acid on adherence of a type strain and 40 clinical isolates of EAggEC to HEp-2 cells and erythrocytes from different species. Growth in the presence of 10 mM salicylate resulted in markedly decreased adherence to tissue culture cells with 33/40 (82.5%) isolates, and was also associated with inhibition of haemagglutination in 20/33 (60.6%) isolates. Complete or partial inhibition of adherence was also seen in two of five isolates showing localised adherence and three of five isolates with diffuse adherence. Decrease in adherence was associated with decreased or absent expression of fimbriae in 28/40 (70%) of the EAggEC isolates, although production of outer membrane proteins was not affected. Salicylates appear to inhibit adherence mediated by fimbrial adhesins.
Wellcome Trust Research Laboratory, I.C.M.R. Centre for Advanced Research on Enteric Diseases, Department of Gastrointestinal Sciences, Vellore, India. cherry@gastro.cmc.ernet.in
G
G
Kang
K A
KA
Balasubramanian
R
R
Koshi
M M
MM
Mathan
V I
VI
Mathan
D000328:Adult; D001422:Bacterial Adhesion / Q000187:drug effects*; D001425:Bacterial Outer Membrane Proteins / Q000302:isolation & purification; D002648:Child; D003967:Diarrhea / Q000382:microbiology; D004847:Epithelial Cells / Q000382:microbiology; D004912:Erythrocytes; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure; D010861:Fimbriae, Bacterial / Q000502:physiology / Q000648:ultrastructure; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D019804:Mesalamine / Q000494:pharmacology*; D008854:Microscopy, Electron; D012980:Sodium Salicylate / Q000494:pharmacology*; D014407:Tumor Cells, Cultured; D014774:Virulence
D001425:Bacterial Outer Membrane Proteins; D019804:Mesalamine; D012980:Sodium Salicylate