Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings.
Presence of homo- and hetero-tetrameric rice sucrose synthase (RSS) isoforms in etiolated rice seedlings was demonstrated by immunoprecipitation using monospecific antibodies. Three RSS isozymes with various pI values were purified by ammonium sulfate fractionation, Sepharose CL-6B gel filtration, DEAE-Sephacel and Mono-Q ion exchange chromatographies. They were characterized as heterotetramers composed of RSS1 and RSS2 subunits. All of them used UDP as the best substrate. The presence of divalent metal ions increased the activities of synthesis but inhibited the cleavage of sucrose.
Department of Agricultural Chemistry, National Taiwan University, Taipei.
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Huang
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Wang
D015153:Blotting, Western; D002118:Calcium / Q000494:pharmacology; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D007526:Isoelectric Point; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D012275:Oryza / Q000201:enzymology*; D011233:Precipitin Tests; D013395:Sucrose / Q000378:metabolism; D014530:Uridine Diphosphate / Q000378:metabolism
D007527:Isoenzymes; D013395:Sucrose; D014530:Uridine Diphosphate; D005964:Glucosyltransferases; C022699:sucrose synthase; D008274:Magnesium; D002118:Calcium