Cholinergic excitation of dendrites in neocortical neurons.
Discharge patterns were studied in response to iontophoretic application of acetylcholine to the soma and dendrites of 128 neocortical pyramidal neurons of layer V. Extracellular recordings were obtained from slices of the guinea-pig parietal cortex. All responses found were excitatory and were better expressed in spontaneously firing cells than in silent ones. Sensitivity to acetylcholine was approximately the same at somatic and dendritic sites in all the cells. Activation of muscarinic receptors gave rise to firing patterns with equal latencies and intensities when applied to both soma and dendrites. The latter suggests that membrane excitation elicited in dendrites by binding of acetylcholine to muscarinic cholinoreceptors is likely to propagate towards the soma through intracellular biochemical processes. Modulating effect of acetylcholine on output firing patterns, elicited by dendritic application of excitatory amino acids, included shortening of the somatic response latency and increase of response intensity and duration. We propose that, in contrast to glutamatergic excitation, the spread of cholinergic excitation along dendrites involves intra-cellular chemical signalling and results in changing the electrical properties of dendrites all over their length.
Institute of Higher Nervous Activity and Neurophysiology, Moscow, Russia.
Y S
YS
Mednikova
S V
SV
Karnup
E V
EV
Loseva
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000502:physiology; D003712:Dendrites / Q000187:drug effects* / Q000502:physiology; D018698:Glutamic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D007478:Iontophoresis; D008564:Membrane Potentials; D019579:Neocortex / Q000166:cytology*; D010296:Parietal Lobe / Q000502:physiology; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D011930:Reaction Time; D011976:Receptors, Muscarinic / Q000187:drug effects* / Q000502:physiology; D009435:Synaptic Transmission
D011976:Receptors, Muscarinic; D001224:Aspartic Acid; D018698:Glutamic Acid; D000109:Acetylcholine