[Indirect ultramicroELISA for the detection of total adenovirus antibodies in human serum].
An indirect ultramicroELISA method was normalized for the detection of total antibodies to Adenovirus (AD) (UMELISA AD). The optimum concentration of surface antigen determined was 30 micrograms/mL; serum dilution was of 1:40; and conjugate dilution, of 1:1,000. The UMELISA AD was compared with the complement fixing and indirect immunofluorescence techniques. Results showed accordance by 88% and 96.6%, respectively. The method is valid for the screening of antibodies in seroepidemiological studies as well as for the diagnosis of the infection by paired serum samples.
Instituto de Medicina Tropical Pedro Kouri.
C
C
Savón
J
J
Laferté
A
A
Goyenechea
A
A
Valdivia
L
L
Morier
Y
Y
Tejero
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002522:Chlorocebus aethiops; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D012680:Sensitivity and Specificity; D014709:Vero Cells
D000914:Antibodies, Viral
UltramicroELISA indirecto para la detección de anticuerpos totales a Adenovirus en suero humano.