Comparison of ELISA and 2-META assays used in serological diagnosis of infection with Actinobacillus pleuropneumoniae serotypes 2 and 4-7 in breeding pigs in Croatia.
The aim of the study was to compare the results of ELISA and 2-META assays in serological diagnosis of swine pleuropneumonia in breeding pigs of different ages. A total of 874 sera were examined for the presence of antibodies against Actinobacillus (A.) pleuropneumoniae serotype 2. The sera had been taken from 437 gilts (aged approximately 6 months), 334 sows (> 2 years of age) and 104 boars (1-2 years). Sixty-nine sera of the gilts and sows, and 46 sera of the boars were also examined for the presence of antibodies against A. pleuropneumoniae serotype 4-7. Sera from the youngest age group (gilts) showed the highest agreement between the results obtained by the two assays, with a kappa value of 0.633 and 0.437 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. The lowest agreement of the two assays was recorded in the oldest age group (sows), with a kappa value of 0.128 and 0.199 for detection of antibodies against A. pleuropneumoniae serotype 2 and 4-7, respectively. Boars were older than gilts and younger than sows. In this group of animals, agreement between the results of the two assays was inferior to that found in gilts and superior to that recorded in sows.
Croatian Veterinary Institute, Department of Bacteriology, Zagreb, Croatia.
B
B
Habrun
V
V
Bilić
A
A
Humski
D000189:Actinobacillus Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification / Q000302:isolation & purification; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001947:Breeding; D017523:Croatia; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D006386:Hemagglutination Tests / Q000662:veterinary*; D008297:Male; D008623:Mercaptoethanol; D011001:Pleuropneumonia / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D012703:Serotyping / Q000379:methods / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology
D000907:Antibodies, Bacterial; D008623:Mercaptoethanol