[Explant test with skin and peritoneum of the neonatal rat as a predictive test of tolerance of local anti-infective agents in wounds and body cavities].
In vitro culture of peritoneal explants of neonatal rats after previous application of agents simulating wound antisepsis is a sensitive screening method for the determination of the tissue compatibility of local wound antiinfectives. Two test models are differentiated: (1) separated peritoneal explants as a model for chronic or deep wounds and (2) peritoneum in situ in the experimental animal with subsequent extraction and cultivation of the explants. Considering the present state of knowledge the following conclusions can be drawn regarding antisepsis of wounds: Lavasept (0.1%) may be classified as the agent of choice for deep and chronic wounds, for drip-suck irrigation and for antiinfective lavage of body cavities inclusively for peritoneal lavage (0.05%). Taurolidin is antiseptically effective in long term application (> 6 h), and because of its antitoxic effect as well as lack of cytotoxicity it is especially suitable for peritoneal lavage. Betaisodona solution is very well suited for superficial contaminated wounds and can be used in a dilution of 1:10 for short-term rinsing of deep wounds, including body cavities but not for peritoneal lavage. Ethanol causes no inhibition of explant growth and therefore retains its importance in wound antisepsis.
Institut für Hygiene und Umweltmedizin, Ernst-Moritz-Arndt-Universität Greifswald.
A
A
Kramer
V
V
Adrian
P
P
Rudolph
S
S
Wurster
H
H
Lippert
D000287:Administration, Topical; D000818:Animals; D000831:Animals, Newborn; D000890:Anti-Infective Agents / Q000633:toxicity*; D000891:Anti-Infective Agents, Local / Q000633:toxicity; D046508:Culture Techniques; D004202:Disinfectants / Q000633:toxicity; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D010537:Peritoneum / Q000187:drug effects*; D051381:Rats; D012867:Skin / Q000187:drug effects*
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D004202:Disinfectants
Explantationstest mit Haut und Peritoneum der neonatalen Ratte als Voraussagetest zur Verträglichkeit lokaler Antiinfektiva für Wunden und Körperhöhlen.