[Detection of Hep-2 and hepatoma cell line chromosomal aberration by using fluorescence in situ hybridization].
OBJECTIVE
To study the chromosomal aberration of hepatoma cell line (1172) and laryngocarcinoma cell line (Hep-2).


METHODS
Fluorescence in situ hybridization (FISH) was used with nine chromosomal special libraries.


RESULTS
The abnormal signals indicated that very complicated aberrations existed in the two cell lines. This suggested that the aberrations also existed in other chromosomes not yet studied. In 1172, the major type was structure aberration, but in Hep-2, numerical abnormality was shown in almost all chromosomes studied.


CONCLUSIONS
Compared with traditional cytological methods, this technique has the advantages of being quicker and more accurate and sensitive.
Department of Biology, Najing Normal University, Nanjing, Jiangsu, 210097 P. R. China.
S
S
Pan
X
X
Zhang
F
F
Wang
W
W
Zhao
C
C
Li
Y
Y
Chen
D002277:Carcinoma / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002460:Cell Line; D002869:Chromosome Aberrations; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics*; D014407:Tumor Cells, Cultured