Spin trapping for nitric oxide produced in LPS-treated mouse using various new dithiocarbamate iron complexes having substituted proline and serine moiety.
Four dithiocarbamate derivatives of 4-substituted L-proline and N-methyl-L-serine were synthesized, and their iron complexes were prepared in Tris-HCl buffer solution. These complexes were used as spin trapping reagents for nitric oxide in ESR spectrometry, and compared with each other in regard to their spin trapping properties in vivo. When the synthesized complexes were injected to lipopolysaccharide-treated mice intravenously, the nitric oxide adducts were detected both in the liver and in the blood except N-dithiocarboxy-4-(methoxymethyl)oxy-L-proline iron complex, whose nitric oxide adduct was detected mostly in the blood. When the exogenous nitric oxide adduct of this complex was injected, it was not detected in the liver, too. It is considered that this complex can trap nitric oxide in the blood by excluding the accumulation of the nitric oxide adduct in the liver.
Nakagawa
H
H
National Institute of Radiological Sciences, Chiba, Japan.
Ikota
N
N
Ozawa
T
T
Masumizu
T
T
Kohno
M
M
D019943:Amino Acid Substitution; D000818:Animals; D005296:Ferrous Compounds; D007275:Injections, Intravenous; D008070:Lipopolysaccharides / Q000633:toxicity*; D008099:Liver / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000097:blood; D011392:Proline; D012694:Serine; D019097:Spin Trapping / Q000379:methods*; D013859:Thiocarbamates
D005296:Ferrous Compounds; D008070:Lipopolysaccharides; D013859:Thiocarbamates; D009569:Nitric Oxide; D012694:Serine; D011392:Proline