Discovering transthyretin amyloid fibril inhibitors by limited screening.
Insoluble protein fibrils, resulting from the self-assembly of a conformational intermediate are implicated to be the causative agent in several human amyloid diseases including familial amyloid polyneuropathy (FAP) and senile systemic amyloidosis (SSA). These diseases are associated with transthyretin (TTR) amyloid fibrils, which appear to form in the acidic partial denaturing environment of a lysosome or endosome. Here we identify several structural classes of small molecules that are capable of inhibiting the TTR conformational changes facilitating amyloid fibril formation. A small molecule inhibitor that stabilizes the normal conformation of a protein is desirable as a promising approach to treat amyloid diseases and to rigorously test the amyloid hypothesis, the apparent causative role of amyloid fibrils in amyloid disease.
Baures
P W
PW
Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA.
Peterson
S A
SA
Kelly
J W
JW
D000682:Amyloid / Q000037:antagonists & inhibitors; D017772:Amyloid Neuropathies / Q000188:drug therapy; D000686:Amyloidosis / Q000188:drug therapy; D000880:Anthraquinones / Q000737:chemistry / Q000494:pharmacology; D001577:Benzophenones / Q000737:chemistry / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D005439:Flufenamic Acid / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D011228:Prealbumin / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship
D000682:Amyloid; D000880:Anthraquinones; D001577:Benzophenones; D005419:Flavonoids; D011228:Prealbumin; D005439:Flufenamic Acid