Transmembrane topology mapping using biotin-containing sulfhydryl reagents.
Seal
R P
RP
Program in Neuroscience, Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201, USA.
Leighton
B H
BH
Amara
S G
SG
D000818:Animals; D001710:Biotin; D019921:Biotinylation / Q000379:methods; D015153:Blotting, Western / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D003545:Cysteine / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007202:Indicators and Reagents; D008565:Membrane Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000378:metabolism*; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D013439:Sulfhydryl Reagents; D014162:Transfection / Q000379:methods
D007202:Indicators and Reagents; D008565:Membrane Proteins; D011994:Recombinant Proteins; D013439:Sulfhydryl Reagents; D001710:Biotin; D003545:Cysteine