[The effect of ionizing radiation and radiosensitizing agent AK-2123 on luminol-dependent chemiluminescence of human neutrophils].
The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.
Shchepetkin
I A
IA
Institute of Oncology, Tomsk Research Center, Russian Academy of Medical Sciences.
D005720:Gamma Rays; D006801:Humans; D007202:Indicators and Reagents / Q000494:pharmacology; D008163:Luminescent Measurements; D008165:Luminol / Q000494:pharmacology; D009504:Neutrophils / Q000737:chemistry / Q000187:drug effects / Q000528:radiation effects*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D014230:Triazoles / Q000494:pharmacology*
D007202:Indicators and Reagents; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D008165:Luminol
Vliianie ioniziruiushchego izlucheniia i radiosensibilizatora AK-2123 na liuminolzavisimuiu khemiliuminestsentsiiu neĭtrofilov cheloveka.