Quantification of early damage in latissimus dorsi muscle grafts.
Acute damage in the latissimus dorsi muscle may account for variable clinical results following dynamic cardiomyoplasty and an ischemic cause has been suggested. Using established techniques, we set out to demonstrate and to quantify the acute muscle damage in a rodent model. The left latissimus dorsi muscle of 5 Sprague-Dawley rats was mobilized on its thoracodorsal vascular pedicle, thus interrupting the regional blood supply to its distal part. The undisturbed contralateral muscle served as a matched control. After 24 hours, the muscle was excised and divided into proximal, middle, and distal thirds. Damage was graded histologically and quantified by nitroblue tetrazolium macrohistochemistry. Both methods of assessment correlated well (r=-0.936; P < 0.001) and demonstrated significant damage, principally in the middle and the distal regions of the ischemic muscles. Therefore, the rodent model appears to be useful for investigating the pathogenesis and prevention of acute ischemic damage in the latissimus dorsi graft under conditions resembling the clinical scenario.
Department of Cardiothoracic Surgery, Wythenshawe Hospital, Manchester, UK.
A T
AT
Tang
P S
PS
Hasleton
H
H
Reid
T L
TL
Hooper
D000818:Animals; D018421:Cardiomyoplasty / Q000009:adverse effects* / Q000379:methods*; D004195:Disease Models, Animal; D006085:Graft Survival / Q000502:physiology; D007511:Ischemia / Q000503:physiopathology*; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000166:cytology; D018482:Muscle, Skeletal / Q000098:blood supply / Q000166:cytology / Q000637:transplantation*; D009580:Nitroblue Tetrazolium; D051381:Rats; D017207:Rats, Sprague-Dawley
D009580:Nitroblue Tetrazolium