[Establishment of partial gene expression map of 7q32 in nasopharyngeal carcinoma and primary culture normal nasopharyngeal epithelial cells].
OBJECTIVE
To establish partial gene expression map of 7q32 in nasopharyngeal carcinoma (NPC) cell line, tissues and primary culture normal nasopharyngeal epithelial cells.


METHODS
We detected the expression of 20 ESTs at 7q32 in NPC cell line HNE1,13 NPC biopsies and primary culture normal nasopharyngeal epithelial cells using differential RT PCR and Northern hybridization.


RESULTS
8 ESTs (AA188181, AA13079,N27556, AA031919, N22721, H20825, T91284, AA001936) expressed equally in both of HNE1 and primary culture normal nasopharyngeal epithelial cells; 7 ESTs (T64215, AA025822, R60014,R80002,H06688, R60192,R95096) expressed in neither of them; 3 ESTs (H19830,W72688,AA130630) overexpressed in HNE1 ; and 2 ESTs (AA070437, H90882) overexpressed in primary culture normal nasopharyngeal epithelial cells. W72688 and H19830 each overexpressed in 77%(10/13) of NPC biopsies; AA070437 down-expressed in 30.7% of NPC biopsies.


CONCLUSIONS
Partial gene expression map of 7q32 in nasopharyngeal carcinoma cell line ,tissues and primary culture normal nasopharyngeal epithelial cells has been established. The up-regulation of W72688, H19830 and down-regulation of AA070437 may be related to the occurrence of NPC.
Jiang
N
N
Cancer Research Institute, Hunan Medical University, Changsha,Hunan, 410078 P. R. China. ligy@public.cs.hn.cn
Zhan
F
F
Xie
Y
Y
Zeng
Z
Z
Zhou
M
M
Deng
L
L
Li
G
G
D015152:Blotting, Northern; D002460:Cell Line; D004847:Epithelial Cells; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D009305:Nasopharynx / Q000166:cytology*; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured