Evaluation of a commercial PCR kit for diagnosis of cytomegalovirus infection of the central nervous system.
We evaluated the AMPLICOR cytomegalovirus (CMV) PCR kit for the diagnosis of neurologic CMV infections on 43 positive and 112 negative archived cerebrospinal fluid specimens originally tested by an in-house PCR method. The AMPLICOR kit showed sensitivity and specificity of 95 and 100%, respectively, versus the home-grown assay, indicating its utility in this clinical setting.
Pediatric Infectious Diseases, University of Colorado School of Medicine, Denver, Colorado, USA. adriana.weinberg@uchsch.edu
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Weinberg
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Spiers
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Cai
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Long
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Sun
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Tevere
D001483:Base Sequence; D002494:Central Nervous System Infections / Q000175:diagnosis* / Q000821:virology*; D002555:Cerebrospinal Fluid / Q000821:virology; D003587:Cytomegalovirus / Q000235:genetics* / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000134:cerebrospinal fluid / Q000235:genetics; D005069:Evaluation Studies as Topic; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods / Q000706:statistics & numerical data
D017931:DNA Primers; D004279:DNA, Viral