Echinococcus multilocularis infection in mice: in vivo treatment with a low dose of IFN-gamma decreases metacestode growth and liver fibrogenesis.
As no antiparasitic drug is definitively efficient in patients with alveolar echinococcosis, the effects of exogenous IFN-gamma on murine Echinococcus multilocularis infection were assessed with regards to the parasite burden, parasite-specific immune responses, and the urinary level of the collagen cross-link pyridinolines. They were analyzed after 3-week treatments with 1 or 5 micrograms of IFN-gamma per day twice a week. The treatment with 1 microgram transiently reduced the liver metacestode load, and the metastase weight as far as 6 weeks after the end of treatment. It slightly increased Th 1-type T cell responses and reduced the excretion of pyridinolines. These results should encourage further study to assess whether the decrease in liver fibrosis leads to an improvement of the efficacy of albendazole therapy. In contrast, the treatment with 5 micrograms increased the liver metacestode load and was less efficient than that with 1 microgram in decreasing pyridinoline excretion. These results incitate to follow up carefully patients with alveolar echinococcosis who are treated with IFN-gamma.
Laboratoire de Parasitologie, Faculté de Médecine, Créteil, France.
M
M
Liance
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S
Ricard-Blum
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I
Emery
R
R
Houin
D A
DA
Vuitton
D000596:Amino Acids / Q000652:urine; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004444:Echinococcosis, Hepatic / Q000188:drug therapy*; D004446:Echinococcus / Q000276:immunology / Q000302:isolation & purification; D006968:Hypersensitivity, Delayed; D007074:Immunoglobulin G / Q000096:biosynthesis; D007371:Interferon-gamma / Q000008:administration & dosage / Q000627:therapeutic use*; D008099:Liver / Q000469:parasitology / Q000473:pathology; D051379:Mice; D008806:Mice, Inbred AKR; D009929:Organ Size; D011994:Recombinant Proteins
D000596:Amino Acids; D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D011994:Recombinant Proteins; C015484:pyridinoline; D007371:Interferon-gamma