[Preliminary study of AP-64 cell line (Aedes pseudoscutellaris) for dengue-1 and -2 virus multiplication].
We study a new arthropod cell line, AP-64, which was obtained from Aedes pseudoscutellaris larvae. The best growth and maintenance media are defined, testing the adequacy of dengue 1 and 2 viruses which become manifest by the appearance of the cytopathogenic effect as syncytiums. The immunofluorescence technique is applied to detect early multiplication of those viruses.
Instituto de Medicina Tropical Pedro Kouri.
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L
Morier Díaz
M R
MR
Alemán Miranda
A
A
Castillo Alvarez
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Pérez Forcada
D000330:Aedes / Q000821:virology; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D003716:Dengue Virus / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D007814:Larva / Q000821:virology; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication
Estudio preliminar de la línea celular AP-64 (Aedes pseudoscutellaris) para la multiplicación de los virus dengue-1 y 2.