The characterisation of thiobarbituric acid reactivity in human plasma and urine.
The specificity of the thiobarbituric acid reaction (TBA) has been investigated using techniques of high-performance thin-layer chromatography and spectrofluorimetry. It was found that malondialdehyde (MDA) derived from different lipid and nonlipid origins formed the same MDA-TBA complex. This complex could be separated from other TBA-reactive compounds by both chromatography and spectrofluorimetry. Normal human plasma and urine both formed an MDA-TBA complex along with other TBA-reactive compounds. In plasma this was associated mainly with phosphatidylcholine and appeared to be peroxidic in reaction. Urine, however, contained polar MDA-forming compounds probably resulting from the oxidation of 2-deoxyaldoses during the acid-heating stage of the TBA test.
Department of Clinical Biochemistry, Whittington Hospital, London, England.
J M
JM
Gutteridge
T R
TR
Tickner
D002855:Chromatography, Thin Layer; D006801:Humans; D015227:Lipid Peroxidation; D008055:Lipids / Q000097:blood / Q000737:chemistry; D008315:Malondialdehyde / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis*
D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde