Crystallization of two hCG-specific monoclonal antibody fragments.
The Fab fragments of two monoclonal antibodies (Fab3A2, Fab6A) raised against epitopes of human chorionic gonadotrophin (hCG) have been crystallized using the vapour-diffusion technique. The Fab3A2 antibody recognises an epitope on the C-terminal peptide of the beta-subunit and the Fab6A a conformational epitope of hCG. Both Fab crystals grow as hexagonal rods from ammonium sulfate solutions. The Fab3A2 crystals belong to space group P3121 with a = b = 74.84, c = 198.2 A and diffract to 1.33 A at the ESRF. The Fab6A crystals are in the space group P3221 with a = b = 129.53, c = 74.40 A and diffract to 2.7 A at the Daresbury SRS. One Fab molecule per asymmetric unit is present in both crystals.
Department of Chemistry, University of Glasgow, Glasgow G12 8QQ, Scotland.
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Fotinou
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Beauchamp
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Emsley
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Black
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DeHaan
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Isaacs
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D006063:Chorionic Gonadotropin / Q000276:immunology*; D003460:Crystallization; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D051379:Mice
D000911:Antibodies, Monoclonal; D006063:Chorionic Gonadotropin; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments