[Effect of silymarin on mouse liver damage, production and activity of tumor necrosis factor].
Tumor necrosis factor (TNF) has been well-characterized as a prominent mediator in the development of liver injury. Effects of silymarin (SB) on mouse liver damage, TNF production and activity were studied. Pretreatment with SB (25-50 mg.kg-1, i.p., bid x 3 d) before the lipopolysaccharides (LPS) injection markedly alleviated liver injury and diminished LPS-induced TNF production in Propionibacterium acnes (PA)-primed mice. SB (12.5-50 micrograms.ml-1) significantly inhibited LPS-induced TNF release from mouse peritoneal macrophage in a concentration-dependent manner. SB(12.5-100 micrograms.ml-1) was also shown to markedly reduce TNF cytotoxicity on human hepatic cell line GSG-7701 and mouse fibroblastic cell line L929 cells concentration-dependently. These results suggest that inhibition of TNF production and its actions may be involved in the mechanism of protective action of SB on liver damage.
Zhang
J P
JP
Research Laboratory of Natural and Synthetic Drugs, College of Pharmacy, Second Military Medical University, Shanghai.
Hu
Z L
ZL
Feng
Z H
ZH
Lin
W
W
Yu
X B
XB
Qian
D H
DH
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism*; D005260:Female; D008070:Lipopolysaccharides; D017737:Macrophages, Peritoneal / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D020011:Protective Agents / Q000494:pharmacology*; D012838:Silymarin / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D008070:Lipopolysaccharides; D020011:Protective Agents; D012838:Silymarin; D014409:Tumor Necrosis Factor-alpha