Structure of double-shelled rice dwarf virus.
Rice dwarf virus (RDV), a member of the Reoviridae family, is a double-stranded RNA virus. Infection of rice plants with RDV reduces crop production significantly and can pose a major economic threat to Southeast Asia. A 25-A three-dimensional structure of the 700-A-diameter RDV capsid has been determined by 400-kV electron cryomicroscopy and computer reconstruction. The structure revealed two distinctive icosahedral shells: a T=13l outer icosahedral shell composed of 260 trimeric clusters of P8 (46 kDa) and an inner T=1 icosahedral shell of 60 dimers of P3 (114 kDa). Sequence and structural comparisons were made between the RDV outer shell trimer and the two crystal conformations (REF and HEX) of the VP7 trimer of bluetongue virus, an animal analog of RDV. The low-resolution structural match of the RDV outer shell trimer to the HEX conformation of VP7 trimer has led to the proposal that P8 consists of an upper domain of beta-sandwich motif and a lower domain of alpha helices. The less well fit REF conformation of VP7 to the RDV trimer may be due to the differences between VP7 and P8 in the sequence of the hinge region that connects the two domains. The additional mass density and the absence of a known signaling peptide on the surface of the RDV outer shell trimer may be responsible for the different interactions between plants and animal reoviruses.
National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China.
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Lu
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Zhou
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Baker
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Jakana
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Wei
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Chen
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Chiu
D000595:Amino Acid Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure; D020285:Cryoelectron Microscopy; D007091:Image Processing, Computer-Assisted; D046911:Macromolecular Substances; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012275:Oryza / Q000821:virology*; D011487:Protein Conformation; D012087:Reoviridae / Q000737:chemistry / Q000235:genetics / Q000648:ultrastructure*; D017386:Sequence Homology, Amino Acid
D046911:Macromolecular Substances