cDNA cloning and expression of a novel serine protease, TLSP.
A cDNA for a putative novel serine protease, TLSP, was cloned from human hippocampus cDNA with polymerase chain reaction based strategies. The putative amino acid sequence of TLSP is similar to the trypsin-type serine proteases. TLSP mRNA is expressed in keratinocytes. Overexpressed TLSP protein in neuro2a cells was detected in culture medium.
Division of Structural Cell Biology, Nara Institute of Science and Technology, Japan.
S
S
Yoshida
M
M
Taniguchi
T
T
Suemoto
T
T
Oka
X
X
He
S
S
Shiosaka
D000595:Amino Acid Sequence; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000737:chemistry; D006624:Hippocampus / Q000201:enzymology*; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology*; D008969:Molecular Sequence Data; D010957:Plasmids; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D014357:Trypsin / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C114880:trypsin-like serine protease; D012697:Serine Endopeptidases; D014357:Trypsin