Identification of atypical strains of Staphylococcus epidermidis by use of molecular tools.
Four atypical coagulase-negative staphylococcal (CNS) isolates from clinical sources were compared with Staphylococcus epidermidis strains by ribotyping. The ribotypes of the four strains shared close rDNA restriction profiles with those of the S. epidermidis strains used. The DNA sequence encoding 16S rRNA demonstrated 99.9% homology with S. epidermidis. S1 nuclease experiments showed that these atypical strains formed a homogeneous genomic group. DNA-DNA homologies between the S. epidermidis type strain CCM 2124 and the four CNS isolates ranged from 70 to 89%. The guanine-plus-cytosine content of the deoxyribonucleic acid of the four strains ranged from 31 to 32 mol%.
Riou
C
C
Département de Recherche en Bactériologie Médicale (UPRES EA 1655), Faculté de Médecine RTH Laennec, Lyon, France.
Meugnier
H
H
Bes
M
M
Brun
Y
Y
Fiedler
F
F
Lasne
Y
Y
Etienne
J
J
Freney
J
J
D000596:Amino Acids / Q000032:analysis; D001295:Atypical Bacterial Forms / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis; D005838:Genotype; D006801:Humans; D015336:Molecular Probe Techniques; D010457:Peptidoglycan / Q000737:chemistry; D010641:Phenotype; D010758:Phosphorus / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics; D013212:Staphylococcus epidermidis / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification
D000596:Amino Acids; D004275:DNA, Ribosomal; D010457:Peptidoglycan; D012336:RNA, Ribosomal, 16S; D010758:Phosphorus