Comparative studies of protein crystallization by vapour-diffusion and microbatch techniques.
Numerous reports have been published in the literature which describe the crystallization of macromolecules by a variety of crystallization methods, including the vapour-diffusion and microbatch techniques. This topical review compares the results of examples of proteins which were crystallized by both vapour-diffusion and microbatch methods. The inherent features of the vapour-diffusion and microbatch methods are discussed and some specific conditions where one method appears more favourable than the other are reported. Guidelines for the conversion of crystallization conditions from vapour diffusion to microbatch (and vice versa) are also presented.
Chayen
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Biophysics Section, Blackett Laboratory, Imperial College of Science, Technology and Medicine, London SW7 2BZ, England. n.chayen@ic.ac.uk
D019747:Aldose-Ketose Isomerases / Q000737:chemistry; D002352:Carrier Proteins; D003460:Crystallization; D004058:Diffusion; D004546:Elapid Venoms / Q000737:chemistry; D008156:Luciferases / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*
D002352:Carrier Proteins; D004546:Elapid Venoms; D010446:Peptide Fragments; D011506:Proteins; C018494:crustacyanins; C020273:dendrotoxin; D008156:Luciferases; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase