Alterations of the PPP2R1B gene in human lung and colon cancer.
The PPP2R1B gene, which encodes the beta isoform of the A subunit of the serine/threonine protein phosphatase 2A (PP2A), was identified as a putative human tumor suppressor gene. Sequencing of the PPP2R1B gene, located on human chromosome 11q22-24, revealed somatic alterations in 15% (5 out of 33) of primary lung tumors, 6% (4 out of 70) of lung tumor-derived cell lines, and 15% (2 out of 13) of primary colon tumors. One deletion mutation generated a truncated PP2A-Abeta protein that was unable to bind to the catalytic subunit of the PP2A holoenzyme. The PP2R1B gene product may suppress tumor development through its role in cell cycle regulation and cellular growth control.
Wang
S S
SS
McDermott Center for Human Growth and Development, The University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA.
Esplin
E D
ED
Li
J L
JL
Huang
L
L
Gazdar
A
A
Minna
J
J
Evans
G A
GA
D000595:Amino Acid Sequence; D002453:Cell Cycle; D002455:Cell Division; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D003110:Colonic Neoplasms / Q000201:enzymology / Q000235:genetics*; D016368:Frameshift Mutation; D016147:Genes, Tumor Suppressor; D006801:Humans; D019656:Loss of Heterozygosity; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D054648:Protein Phosphatase 2; D017384:Sequence Deletion; D014407:Tumor Cells, Cultured
D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2