Shigella and enteroinvasive Escherichia coli strains are derived from distinct ancestral strains of E. coli.
The differentiation between Shigella subspecies, and the phylogenetic position of Shigella clones within Escherichia coli clones was determined by analysis of restriction fragment length polymorphisms of rDNA (ribotyping). Seventy-five Shigella strains belonging to the four subspecies and 13 enteroinvasive E. coli (EIEC) strains were compared with the 72 E. coli strains of the ECOR collection, which have been classified into four phylogenetic groups (A, B1, B2 and D). Seventeen Shigella dysenteriae ribotypes, 12 Shigella flexneri ribotypes, 23 Shiegella boydii ribotypes, 12 Shigella sonnei ribotypes and 13 EIEC ribotypes were identified following digestion with HindIII and EcoRI. Correspondence analysis of the data showed that S. boydii serotype 13 strains were distantly related to the other Shigella strains, and that S. sonnei and S. flexneri were distinct from S. boydii and S. dysenteriae. The ribotypes of Shigella and ECOR strains were indistinguishable, and S. sonnei, S. flexneri and most S. dysenteriae strains were closely related to phylogenetic group D, whereas S. dysenteriae serotype 1 strains belonged to phylogenetic group B1, and S. boydii strains were evenly distributed between the two groups. The Shigella strains were distantly related to group B2, which contains E. coli strains frequently implicated in extra-intestinal infections in humans. In contrast, the 13 EIEC strains were more widely distributed between phylogenetic groups B1, A and B2. Thus, there was no primordial Shigella species and Shigella and EIEC strains are derived from different ancestral strains.
Rolland
Karine
K
Laboratoire de Microbiologie, Facult de Mdecine de Brest22 Avenue Camille Desmoulins, B.P. 815, 29285 Brest CedexFrance.
Lambert-Zechovsky
Nicole
N
Laboratoire de MicrobiologieHoKpital Beaujon, ClichyFrance.
Picard
Bertrand
B
Laboratoire de Microbiologie, Facult de Mdecine de Brest22 Avenue Camille Desmoulins, B.P. 815, 29285 Brest CedexFrance.
Denamur
Erick
E
INSERM U 458, HoKpital Robert Debr, ParisFrance.
D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004751:Enteritis / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology; D005798:Genes, Bacterial; D006801:Humans; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012760:Shigella / Q000145:classification / Q000235:genetics*; D013045:Species Specificity; D014774:Virulence / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal