Effects of sustained flow reduction on postnatal intestinal circulation.
Studies were conducted to determine the effect of mechanically induced sustained flow reduction on intestinal hemodynamics and oxygenation in 3- and 35-day-old swine. In vitro gut loops were perfused under controlled-pressure conditions from an oxygenated blood reservoir at age-appropriate perfusion pressures; pressure was rapidly reduced to a level that lowered flow rate to approximately 50% of its baseline value, and pressure was then kept at that level for 2 h. In 3-day-old intestine, vascular resistance (Ri) increased by 20% immediately after pressure and flow reduction but then stabilized for 3-4 min; thereafter, flow began to decrease despite maintenance of perfusion pressure, so that Ri increased an additional 15% by 30 min after flow reduction. Flow continued to diminish over the next 90 min, though at much slower rate. Intestine from 35-day-old swine demonstrated an immediate increase in Ri after pressure and flow reduction, but thereafter Ri increased very little. The protocol was repeated within in vitro gut loops perfused under controlled-flow conditions, and within autoperfused, innervated gut loops developed in vivo and similar observations were made in both preparations. In 3-day-old intestine, pretreatment with the L-arginine analog Nomega-monomethyl-L-arginine (10(-4) M) had no effect on the immediate rise in resistance occurring in the first 1 min but substantially attenuated the subsequent slow, progressive rise noted thereafter. Pretreatment with the angiotensin 1A receptor antagonist losartan (2 x 10(-6) M) had no effect on hemodynamic changes during the first 60 min after mechanical perfusion pressure reduction but attenuated the very slight increase in resistance noted during the final 60 min of the protocol. The postnatal intestinal circulation demonstrates progressive vasoconstriction when its flow rate is mechanically reduced in a sustained manner, and this effect is age specific, occurring in 3- but not 35-day-old swine. These changes in gut vascular resistance may be consequent to loss of nitric oxide production and/or local production of angiotensin.
Department of Pediatrics, The Ohio State University, Columbus 43210; and Wexner Institute for Pediatric Research, Childrens Hospital, Columbus, Ohio 43205, USA.
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D000375:Aging / Q000502:physiology*; D000803:Angiotensin I / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001794:Blood Pressure / Q000187:drug effects; D006439:Hemodynamics / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D036341:Intercellular Signaling Peptides and Proteins; D007421:Intestine, Small / Q000098:blood supply* / Q000254:growth & development; D007537:Isometric Contraction / Q000187:drug effects; D008638:Mesenteric Arteries / Q000254:growth & development / Q000502:physiology*; D024510:Muscle Development; D009130:Muscle, Smooth / Q000098:blood supply / Q000254:growth & development; D009131:Muscle, Smooth, Vascular / Q000254:growth & development / Q000502:physiology*; D010101:Oxygen Consumption; D010455:Peptides / Q000494:pharmacology; D010477:Perfusion; D012039:Regional Blood Flow; D013552:Swine; D014655:Vascular Resistance; D019323:omega-N-Methylarginine / Q000494:pharmacology
D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D000804:Angiotensin II; D019323:omega-N-Methylarginine; D000803:Angiotensin I; C042955:H-asparaginyl-arginyl-valyl-tyrosyl-isoleucyl-histyl-prolyl-phenylalanyl-histyl-leucyl-valyl-isoleucyl-serine