[Detection of anti-Histoplasma capsulatum antibodies by the ELISA technique. Preliminary study].
An indirect micro-ELISA system is presented for diagnosing histoplasmosis. The diagnostic criteria are defined by using sera from 12 patients who are histoplasmosis carriers. For this group, the optical density values were superior to 1,000; use was made of 43 sera from blood bank donors and 9 sera from children without a history of exposure. The optical density values in these cases were inferior to 0,200. The significant difference found led to the diagnostic criterion for confirming 3 histoplasmosis carriers who showed clinical manifestations but had been negative to double immunodiffusion. Thus, the usefulness of the proposed micro-ELISA system for early diagnosis was proved.
Instituto de Medicina Tropical Pedro Kouri.
C
C
Fernández Andreu
G
G
Martínez Machín
R
R
Fernández Llanes
A
A
Llop Hernández
D000908:Antibodies, Fungal / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006658:Histoplasma / Q000276:immunology*; D006660:Histoplasmosis / Q000097:blood / Q000276:immunology*; D006801:Humans; D008172:Lung Diseases, Fungal / Q000097:blood / Q000276:immunology*
D000908:Antibodies, Fungal
Detección de anticuerpos anti-Histoplasma capsulatum mediante la técnica de ELISA. Estudio preliminar.