A surface plasmon resonance study of albumin adsorption to PEO-PPO-PEO triblock copolymers.
Pluronic surfactants, PEO-PPO-PEO triblock copolymers, have been investigated widely due to their protein-resistant properties in applications as coatings for implants and in controlled drug release systems. We have studied a wide range of these copolymers, varying in both PEO and PPO block size, by adsorbing them to a polystyrene surface and investigating their subsequent resistance to human serum albumin adsorption. This investigation has been carried out in real time, using surface plasmon resonance, with the surfaces subsequently visualized by atomic force microscopy. This approach has allowed determination of the effect of the lengths of the PEO and PPO polymer chains on protein resistivity. For low-molecular-weight Pluronics a significant, yet not complete, reduction in albumin adsorption has been observed whereas higher molecular weight Pluronics appear to completely inhibit adsorption within the time frame of this experiment. An increase in the PPO block size of the copolymer also appears to increase its protein resistance. This work further confirms that the binding strength of the anchoring block to the hydrophobic surface, rather than the length of the protruding hydrophilic PEO chains, determines a copolymer's protein resistance capability.
Department of Pharmaceutical Sciences, The University of Nottingham, United Kingdom.
R J
RJ
Green
M C
MC
Davies
C J
CJ
Roberts
S J
SJ
Tendler
D000418:Albumins / Q000737:chemistry*; D001672:Biocompatible Materials; D006801:Humans; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011409:Propylene Glycols / Q000737:chemistry*; D011485:Protein Binding; D020349:Surface Plasmon Resonance
D000418:Albumins; D001672:Biocompatible Materials; C116176:PEO-PPO-PEO; D011108:Polymers; D011409:Propylene Glycols; D011092:Polyethylene Glycols