Synergistic approaches based on nonchromatographic continuous separation techniques (solid-phase extraction and pervaporation) and chromatography couplings.
Approaches based on continuous separation units coupled to either liquid or gas chromatography for improving the features of analytical methods are proposed. Examples of solid-phase separation-liquid chromatography for the determination of fat-soluble vitamins and their metabolites in clinical samples, and pervaporation-gas chromatography for the determination of volatile compounds in solid environmental samples are described. The clean-up and preconcentration effect achieved by the former coupling and the easy and effective solid-sample pretreatment in the latter clearly show their utility. The use of pervaporation as an advantageous alternative to headspace is demonstrated.
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
M D
MD
Luque de Castro
J M
JM
Fernández-Romero
D002762:Cholecalciferol / Q000031:analogs & derivatives / Q000097:blood / Q000302:isolation & purification; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans
D002762:Cholecalciferol