[Biosynthesis of leucine aminopeptidase by Xanthomonas rubrilineans 67 in culture with various concentrations of nitrogen containing compounds].
The dynamics of consumption of amine and ammonium nitrogen and glucose in the process of Xanthomonas rubrilineans 67 growth and biosynthesis of leucine aminopeptidase was studied. It was shown that the rate of leucine, alanine and glycine consumption as a source of amine nitrogen out of 16 amino acids was the highest during the fermentation. Addition of these three amino acids or their mixtures to the medium at definite stages of the fermentation process increased the leucine aminopeptidase biosynthesis by 50 to 100 per cent. Ammonium nitrogen was not used by X.rubrilineans 67. The consumption of glucose during the fermentation was even: by the 24th hour of the process the medium contained about 10 per cent of the glucose initial concentration. The optimal temperature for the culture growth and leucine aminopeptidase biosynthesis was determined. It was shown to be 28 degrees C. Higher aeration increased the culture productivity.
I.M. Sechenov Moscow Medical Academy, National Research Centre of Antibiotics, Moscow.
E G
EG
Koniaeva
I N
IN
Krest'ianova
V P
VP
Ivanov
A V
AV
Shvets
M V
MV
Baturina
D000409:Alanine / Q000378:metabolism; D000588:Amines / Q000378:metabolism; D003470:Culture Media; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D007931:Leucyl Aminopeptidase / Q000096:biosynthesis*; D009584:Nitrogen / Q000378:metabolism*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism; D013696:Temperature; D014974:Xanthomonas / Q000201:enzymology / Q000254:growth & development*
D000588:Amines; D003470:Culture Media; D000644:Quaternary Ammonium Compounds; D007931:Leucyl Aminopeptidase; D007930:Leucine; D005947:Glucose; D009584:Nitrogen; D000409:Alanine; D005998:Glycine
Biosintez leĭtsinaminopeptidazy kul'turoĭ Xanthomonas rubrilineans 67 pri var'irovanii azotosoderzhashchikh komponentov sredy.