Lipoxygenase-1 from soybean seed inhibiting the activity of pancreatic lipase.
There are some similar characteristics in protein nature between the lipase inhibitor from soybean seed and soybean lipoxygenase-1 (LOX-1). Thus, the inhibiting protein for pancreatic lipase was prepared from defatted soybean meal by the procedure for the isolation of LOX-1 [Axelrod et al., Methods in Enzymology, 71, 441-451 (1981)]. The LOX-1 from soybean seed dose-dependently inhibited the release of fatty acid from a soybean oil emulsion, and the concentration of LOX-1 to cause half inhibition of the lipase activity was 3.2 x 10(-7) M. The LOX-1 obtained from E. coli transfected with a plasmid carrying the soybean LOX-1 gene also inhibited the lipase activity. However, the lipase-inhibiting activity by the LOX-1 was not affected by the presence of nordihydroguaiaretic acid, an inhibitor for LOX, in the reaction mixture. These results show that the soybean LOX-1 inhibits lipase activity regardless of its lipoxygenase activity.
Satouchi
K
K
Department of Food Science and Technology, Fukuyama University, Japan. satouchi@fubac.fukuyama-u.ac.jp
Hirano
K
K
Fujino
O
O
Ikoma
M
M
Tanaka
T
T
Kitamura
K
K
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004655:Emulsions; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry; D008041:Linoleic Acids / Q000032:analysis; D008049:Lipase / Q000037:antagonists & inhibitors*; D008054:Lipid Peroxides / Q000032:analysis; D008084:Lipoxygenase / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D016859:Lipoxygenase Inhibitors / Q000494:pharmacology; D009637:Masoprocol / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology*; D012639:Seeds / Q000201:enzymology* / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology* / Q000378:metabolism; D013053:Spectrophotometry
D004655:Emulsions; D005230:Fatty Acids, Nonesterified; D008041:Linoleic Acids; D008054:Lipid Peroxides; D016859:Lipoxygenase Inhibitors; C008285:linoleic acid hydroperoxide; D009637:Masoprocol; D008084:Lipoxygenase; D008049:Lipase