An agarose gel electrophoretic method for analysis of sulfated glycosaminoglycans of cultured cells.
Agarose disc gel electrophoresis has been adapted to achieve the separation of the major sulfated glycosaminoglycans produced by cells in culture. By use of buffers containing barium ion, mixtures of chondroitin sulfate, dermatan sulfate, and heparan sulfate are well resolved into discrete bands. The technique can be used preparatively as well as analytically to separate quantities of glycosaminoglycans up to a milligram in a 6-mm diameter gel.
Department of Ophthalmology, University of Washington, Seattle 98195, USA.
J L
JL
Funderburgh
J W
JW
Chandler
D000818:Animals; D001464:Barium; D002417:Cattle; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000032:analysis; D003871:Dermatan Sulfate / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis
D001464:Barium; D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate