The homeobox gene NTH23 of tobacco is expressed in the basal region of leaf primordia.
We reported isolation and characterization of a homeobox gene from tobacco, NTH23. The homeodomain structure of NTH23 was highly homologous to the same regions of class 2 genes of the KN1-type homeobox (sharing more than 85% amino acid identity), but was less similar to class 1 genes of KN1-type. RNA gel blot analysis revealed that NTH23 was expressed in all organs we tested although the gene is primarily expressed in young leaves. To determine more precisely the spatial expression pattern of NTH23 in tobacco, a chimeric NTH23::GUS fusion gene was introduced into tobacco. The signal of GUS activity was observed at the basal part of leaf blade primordia in the NTH23::GUS transgenic tobacco plants. This observation suggests the possibility that NTH23 may be important for the lateral growth of leaf blades.
BioScience Center, Nagoya University, Aichi, Japan.
N
N
Sentoku
M
M
Tamaoki
A
A
Nishimura
M
M
Matsuoka
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D005801:Genes, Homeobox; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D010947:Plants, Toxic; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D014026:Nicotiana / Q000235:genetics*
D018076:DNA, Complementary; D018398:Homeodomain Proteins; C114858:NTH23 protein, Nicotiana tabacum; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins