Intestinal lactase-phlorizin hydrolase (LPH): the two catalytic sites; the role of the pancreas in pro-LPH maturation.
Brush border lactase-phlorizin hydrolase carries two catalytic sites. In the human enzyme lactase comprises Glu-1749, phlorizin hydrolase Glu-1273. The proteolytic processing of pro-lactase-phlorizin hydrolase by (rat) enterocytes stops two amino acid residues short of the N-terminus of 'mature' final, brush border lactase-phlorizin hydrolase. Only these two amino acid residues are removed by luminal pancreatic protease(s), probably trypsin.
Department of Biochemistry, Swiss Federal Institute of Technology, ETH Zentrum, Zurich.
L
L
Zecca
J E
JE
Mesonero
A
A
Stutz
J C
JC
Poirée
J
J
Giudicelli
R
R
Cursio
S M
SM
Gloor
G
G
Semenza
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D004789:Enzyme Activation; D006801:Humans; D007422:Intestines / Q000201:enzymology*; D010694:Lactase-Phlorizin Hydrolase / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010179:Pancreas / Q000201:enzymology*
D010694:Lactase-Phlorizin Hydrolase