Detection of duck enteritis virus by polymerase chain reaction.
Duck enteritis virus (DEV), a herpesvirus, is the causative agent of duck viral enteritis in free-flying, feral, and domesticated members of the Anatidae family. HindIII-digested DEV DNA was cloned into the plasmid pBluescript, and a 1.95-kb fragment was sequenced. This fragment codes for the 3' region of the DEV homologues of varicella-zoster virus (VZV) open reading frame (ORF) UL6 and the 5' region of VZV UL7. Alignment of the putative peptide fragments for DEV UL6 and UL7 showed a 64% and 37% identity with VZV UL6 and UL7, respectively. Primers located in the highly conserved domain of the UL6 gene were used for a polymerase chain reaction (PCR) assay, which was able to amplify DEV DNA. The PCR assay also amplified DEV DNA from the original outbreak samples and/or after passage in Muscovy duck embryos.
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
P J
PJ
Plummer
T
T
Alefantis
S
S
Kaplan
P
P
O'Connell
S
S
Shawky
K A
KA
Schat
D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000235:genetics; D004372:Ducks / Q000821:virology*; D006564:Herpesviridae / Q000235:genetics*; D006566:Herpesviridae Infections / Q000235:genetics / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011201:Poultry Diseases / Q000235:genetics / Q000821:virology*; D016415:Sequence Alignment
D004279:DNA, Viral