[RAPD typing of Campylobacter jejuni and comparison with Lior's or Penner's serotyping system].
Campylobacter jejuni were isolated from 7 epidemic outbreaks (121 isolates), 15 patients with gastroenteritis, chicken meats (47 isolates) and chicken cecal contents (70 isolates). The isolates and one standard strain of C. jejuni JCM2013 were analysed by randomly amplified polymorphic DNA method (RAPD). Total of 254 C. jejuni isolates were divided 68 different RAPD types which included strains that did not to divided by Lior's or Penner's serotyping system. To compare the similarities of RAPD patterns among the isolates, the amplification patterns of DNA were estimated by means of the Dice coefficient, and clustering of strains was based on the unweighted average pair group method (UPGMA) to facilitate the plotting of a dendrogram. It suggests that amplification band patterns of human isolates were different from those of chicken ones. Thus additional information given from RAPD profiles serves for epidemiological investigation and RAPD analysis is recommended as rapid and effective typing method.
Section of Food Microbiology, Saitama Institute of Public Health.
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Ono
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Yamamoto
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Niwa
D000818:Animals; D015373:Bacterial Typing Techniques; D002169:Campylobacter Infections / Q000453:epidemiology / Q000382:microbiology; D016123:Campylobacter jejuni / Q000145:classification* / Q000302:isolation & purification; D002645:Chickens; D004196:Disease Outbreaks; D005517:Foodborne Diseases / Q000453:epidemiology / Q000382:microbiology; D006801:Humans; D007564:Japan / Q000453:epidemiology; D011202:Poultry Products / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping