Structure refinement against synchrotron Laue data: strategies for data collection and reduction.
The synchrotron Laue technique has been applied to high-resolution structure refinement of the ribotoxin, restrictocin [Yang & Moffat (1996). Structure, 4, 837-852]. By employing carefully designed data-collection strategies and the data-reduction algorithms incorporated in the software system LaueView [Ren & Moffat (1995a). J. Appl. Cryst. 28, 461-481; Ren & Moffat (1995b). J. Appl. Cryst. 28, 482-493], a set of high-resolution Laue data with a completeness and accuracy comparable to excellent monochromatic data was obtained. Through detailed comparison with the monochromatic data and electron-density maps derived from the Laue data, optimum data-collection and reduction strategies were identified and the application of Laue diffraction techniques to conventional crystallographic refinement was demonstrated.
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
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D000465:Algorithms; D018360:Crystallography, X-Ray / Q000379:methods*; D003625:Data Collection; D003627:Data Interpretation, Statistical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017356:Synchrotrons