Crystallization and preliminary X-ray analysis of the 12S form of phosphofructokinase from Saccharomyces cerevisiae.
The tetrameric 12S form of yeast phosphofructokinase, obtained by limited proteolytic cleavage of the native enzyme, was crystallized under a variety of conditions. The crystals have been characterized in the X-ray beam and are suitable for crystallographic studies.
Obmolova
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European Molecular Biology Laboratory (EMBL), c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
Kopperschläger
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Heinisch
J
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Rypniewski
W R
WR
D003460:Crystallization; D010732:Phosphofructokinase-1 / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014961:X-Ray Diffraction
D010732:Phosphofructokinase-1