Evaluation of strategies for molecular fingerprinting for use in the routine work of a Mycobacterium reference unit.
We have investigated the role of two rapid PCR-based typing methods, IS6110-based PCR and spacer-oligonucleotide typing, within a national tuberculosis reference service. The validity of clusters with IS6110 restriction fragment length polymorphism fingerprints with less than 6 bands was also investigated in the context of referred isolates.
Public Health Laboratory Service Mycobacterium Reference Unit, Dulwich Hospital, East Dulwich Grove, East Dulwich, London SE22 8QF, United Kingdom. stuart.wilson@kcl.ac.uk
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Wilson
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Goss
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Drobniewski
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004196:Disease Outbreaks; D005069:Evaluation Studies as Topic; D006801:Humans; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology; D006113:United Kingdom / Q000453:epidemiology
D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial