Evidence for the internal location of actin in the pseudorabies virion.
Pseudorabies virions were purified by sucrose gradient and virion-associated proteins were examined. Cytoskeleton actin was found to be a component of virion preparation. In addition, abundant virion-associated actin was detected even after the virion preparation was treated with trypsin digestion or the viral envelope was removed by Triton X-100. This finding indicated that the location of actin is inside the pseudorabies virion. Furthermore, the possible involvement of actin in the life cycle of pseudorabies virus was studied by using cytochalasin D, an F-actin binding drug, and the result showed that cytochalasin D reduced the number of plaques and the size of the plaque of pseudorabies virus.
Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan, ROC.
M L
ML
Wong
C H
CH
Chen
D000199:Actins / Q000032:analysis*; D000818:Animals; D002417:Cattle; D002460:Cell Line / Q000821:virology; D015638:Cytochalasin D / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D011558:Herpesvirus 1, Suid / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification; D015151:Immunoblotting; D011092:Polyethylene Glycols; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis*; D014771:Virion / Q000737:chemistry* / Q000187:drug effects / Q000302:isolation & purification
D000199:Actins; D014764:Viral Proteins; D015638:Cytochalasin D; D011092:Polyethylene Glycols; D014357:Trypsin