Bean Dwarf mosaic geminivirus movement proteins recognize DNA in a form- and size-specific manner.
Plant viral movement proteins mediate the cell-to-cell movement of nucleic acids. This involves either a direct interaction between the viral movement protein and the nucleic acid or an indirect interaction involving host factors. The bipartite geminiviruses possess two movement proteins, BV1 and BC1, that coordinate movement of viral DNA across nuclear and plasmodesmal boundaries, respectively. Here, we demonstrate that both BV1 and BC1 interact directly with DNA and, in addition, that they have the unique property to recognize DNA on the basis of form and size rather than sequence. This is a novel feature for plant virus movement proteins and raises the possibility that BV1 and BC1 may be determinants of genome size in the bipartite geminiviruses.
Division of Biological Sciences, University of California, Davis 95616, USA.
M R
MR
Rojas
A O
AO
Noueiry
W J
WJ
Lucas
R L
RL
Gilbertson
D004270:DNA, Circular / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D007887:Fabaceae / Q000821:virology; D017791:Geminiviridae / Q000378:metabolism*; D053830:Plant Viral Movement Proteins; D010946:Plants, Medicinal; D014764:Viral Proteins / Q000378:metabolism*
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004268:DNA-Binding Proteins; D053830:Plant Viral Movement Proteins; D014764:Viral Proteins