A stomatin-like protein encoded by the slp gene of Rhizobium etli is required for nodulation competitiveness on the common bean.
Rhizobium etli strain TAL182 is a competitive strain for effective nodulation of beans. From this strain, a novel gene was isolated, slp, which is 669 bp in size and required for nodulation competition on the common bean. The slp knockout mutant of TAL182 is defective in nodulation competition, shows reduced growth in the presence of 200 mM NaCl, KCl or LiCl and is complemented by the cloned slp gene. The deduced amino acid sequence of slp shows 66-72% similarity to stomatin proteins of Homo sapiens, Mus musculus and Caenorhabditis elegans. Expression of slp in Escherichia coli from a T7 promoter shows a 26 kDa protein which cross-reacts with human-stomatin-specific polyclonal antibody. Like the human stomatin protein, the slp-deduced protein, Slp, is very hydrophilic except for a single hydrophobic membrane-spanning domain. Among various bean-nodulating rhizobia, slp is present in R. etli, Rhizobium leguminosarum bv. phaseoli and Rhizobium tropici type A strains but is absent in R. tropici type B strains. It is also absent in Bradyrhizobium and several other Rhizobium spp.
Departments of Microbiology, University of HawaiiHonolulu, HI 96822USA.|Departments of Plant Molecular Physiology HonoluluHI 96822USA.
Zerong
Z
You
Departments of Plant Molecular Physiology HonoluluHI 96822USA.
Xuefeng
X
Gao
Department of Medicine, Rayne InstituteLondon WC1E 6JJUK.
Mei M
MM
Ho
Departments of Plant Molecular Physiology HonoluluHI 96822USA.
Dulal
D
Borthakur
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D001798:Blood Proteins / Q000235:genetics*; D017173:Caenorhabditis elegans / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D006801:Humans; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009586:Nitrogen Fixation / Q000235:genetics; D010946:Plants, Medicinal; D012231:Rhizobium / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D013559:Symbiosis
D001426:Bacterial Proteins; D001798:Blood Proteins; D017931:DNA Primers; C488964:Stom protein, mouse; D008565:Membrane Proteins; C036860:STOM protein, human