New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction.
An adenine-to-guanine mutation at nucleotide position (np) 3243 in the mitochondrial tRNALeu(UUR) gene is closely associated with various clinical phenotypes of diabetes mellitus. Because the mutation creates a new restriction site for the restriction enzyme ApaI, the mutation is usually detected and quantified by ApaI cleavage of the PCR products including np 3243. The sensitivity of the conventional method is, however, 5-10% heteroplasmy. The percentage of heteroplasmy of the mutation is usually highest in the affected tissues and is much lower in peripheral blood cells, which are used most frequently for the analysis. The sensitivity of the conventional method, however, is not sufficient to detect the mutation from peripheral blood cells. Utilizing ligation-mediated polymerase chain reaction, we have developed a feasible and sensitive method to detect 0.01% heteroplasmy of the 3243 mutation in peripheral leukocytes.
Clinical Laboratory, Kyushu University Hospital, Fukuoka, Japan.
M
M
Urata
M
M
Wakiyama
M
M
Iwase
M
M
Yoneda
S
S
Kinoshita
N
N
Hamasaki
D
D
Kang
D000225:Adenine / Q000737:chemistry*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002460:Cell Line; D004272:DNA, Mitochondrial / Q000097:blood / Q000737:chemistry / Q000235:genetics*; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics*; D005260:Female; D006147:Guanine / Q000737:chemistry*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D004272:DNA, Mitochondrial; D006147:Guanine; D000225:Adenine