Effects of microheterogeneity in hen egg-white lysozyme crystallization.
In earlier sodium dodecylsulfate-polyacylamide gel electrophoresis (SDS-PAGE) studies it has been found that commonly utilized commercial hen egg-white lysozyme (HEWL) preparations contained 0.2-0.4 mol% covalently bound dimers. Here it is shown, using high-performance capillary electrophoresis (HPCE), that HEWL contains, in addition, two differently charged monomers in comparable amounts. To explore the origin of these microheterogeneous contaminants, purified HEWL (PHEWL) has been oxidized with hydrogen peroxide (0.0026-0.88 M) at various pH levels between 4.5 and 12.0. Optical densitometry of oxidized PHEWL (OHEWL) bands in SDS-PAGE gels shows that hydrogen peroxide at 0.88 M in acetate buffer pH 4.5 increased the amount of dimers about sixfold over that in commercial HEWL. OHEWL had, in addition to one of the two monomer forms found in HEWL and PHEWL, three other differently charged monomer forms, each of them representing about 25% of the preparation. SDS-PAGE analysis of OHEWL yielded two closely spaced dimer bands with Mr = 28000 and 27500. In addition, larger HEWL oligomers with Mr = 1.7 million and 320000 were detected by gel-filtration fast protein liquid chromatography with multiangle laser light scattering detection. Non-dissociating PAGE in large pore size gels at pH 4.5 confirmed the presence of these large oligomers in HEWL and OHEWL. Increased microheterogeneity resulted in substantial effects on crystal growth and nucleation rate. On addition of 10 microgram-1 mg ml-1 OHEWL to 32 mg ml-1 HEWL crystallizing solutions, both the number and size of forming crystals decreased roughly proportionally to the concentration of the added microheterogeneity. The same effect was observed in HEWL solutions on addition of 0.03-0.3 M hydrogen peroxide. Repartitioning of the dimer during crystallization at various temperatures between 277 and 293 K was analyzed by SDS-PAGE. The crystals contained </= 25%(w/w) of the oligomers in the solution, with no apparent temperature dependence of the repartitioning.
Thomas
B R
BR
Center for Microgravity and Materials Research, University of Alabama in Huntsville, Huntsville, Alabama 35899, USA. brtprotein@aol.com
Vekilov
P G
PG
Rosenberger
F
F
D000818:Animals; D002645:Chickens; D003460:Crystallization; D019281:Dimerization; D004563:Electrochemistry; D006861:Hydrogen Peroxide; D008970:Molecular Weight; D009113:Muramidase / Q000737:chemistry* / Q000302:isolation & purification*; D016877:Oxidants; D010084:Oxidation-Reduction; D011487:Protein Conformation
D016877:Oxidants; D006861:Hydrogen Peroxide; D009113:Muramidase