Quantitative Limulus lysate assay for endotoxin activity: aggregation of radioiodinated coagulogen monomers.
This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.
Department of Internal Medicine, University of Texas Health Science Center, Southwestern Medical School, Dallas 75235, USA.
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Munford
D000818:Animals; D001798:Blood Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D006737:Horseshoe Crabs; D007457:Iodine Radioisotopes; D008033:Limulus Test / Q000379:methods*; D008070:Lipopolysaccharides / Q000032:analysis*
D001798:Blood Proteins; D007457:Iodine Radioisotopes; D008070:Lipopolysaccharides; C013445:coagulogen