Plasma source mass spectrometry in experimental nutrition.
The development and commercial availability of plasma ion source, specifically inductively coupled plasma, mass spectrometers (ICP-MS) have significantly extended the potential application of stable isotopes for nutritional modeling. The status of research and commercial ICP-MS instruments, and their applications and limitations for stable isotopic studies are reviewed. The consequences of mass spectroscopic resolution and measurement sensitivity obtainable with quadrupole, sector, time-of-flight, and trap instruments on stable isotope analysis are examined. Requirements for reliable isotope measurements with practical biological samples including tissues and fluids are considered. The possibility for stable isotope analysis in chemically separated compounds (speciation) also is explored. On-line compound separations by chromatography or electrophoresis, for example, have been combined instrumentally with ICP-MS. Som possibilities and requirements are described for stable isotope speciation analysis.
Department of Chemistry, Lederle Graduate Research Center, University of Massachusetts, Amherst, USA.
R M
RM
Barnes
D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D007554:Isotopes / Q000032:analysis*; D007854:Lead / Q000097:blood; D008954:Models, Biological; D014116:Toxicology
D007554:Isotopes; D007854:Lead