Structure determination of the phiX174 closed procapsid.
The structure of a procapsid of the single-stranded DNA bacteriophage ++phiX174 was determined to 3.5 A resolution. The crystal space group was I213 with a unit-cell length of 774 A. The unit cell contained 16 icosahedral virus particles, each situated on a crystallographic threefold axis. Thus, there are two independent one-thirds of a particle per asymmetric unit, and a total of 40-fold non-crystallographic redundancy. To aid in the interpretation of the packing arrangement, crystals were prepared for thin sectioning and analyzed by electron microscopy. Oscillation X-ray diffraction data was collected on image plates using synchrotron radiation and oscillation angles of either 0.25 or 0.30 degrees. A low-resolution 6.5 A data set collected from a single frozen crystal was particularly helpful in the structure determination, because of its completeness and internal consistency. The initial particle orientations were determined using self-rotation functions, while the initial position of one particle was determined from a Patterson map. The structure was solved by molecular replacement real-space averaging using a model based on a cryo-electron microscopy reconstruction as a starting point for the phase determination. The initial structure determination used the data between 20 and 13 A resolution, which was then extended one reciprocal lattice point at a time to 6.5 A resolution. At this point, a 3.5 A resolution data set compiled from a number of crystals collected at 277 K was introduced. Phase extension and averaging continued to 3.5 A resolution after re-determining the particle positions and orientations. The amino-acid sequences of most of the D, F and G proteins and part of the B protein could be unambiguously built into the 3.5 A electron-density map. Partial crystallographic refinement yielded an R factor of 31.6%, consistent with the relatively low resolution and lack of completeness of the data.
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
T
T
Dokland
R
R
McKenna
D M
DM
Sherman
B R
BR
Bowman
W F
WF
Bean
M G
MG
Rossmann
D010584:Bacteriophage phi X 174 / Q000737:chemistry*; D002213:Capsid / Q000737:chemistry* / Q000302:isolation & purification; D003198:Computer Simulation; D003460:Crystallization; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D011487:Protein Conformation; D012399:Rotation; D013696:Temperature