A human B cell line AF10 expressing HIL-17.
AF10, a human B cell line, is a mycoplasma-free variant cloned from the human IgE myeloma cell line U266. Total RNA isolated from the AF10 cells was used as template for RT-PCR, and a specific product of about 411bp corresponding to the coding region of hIL-17 lack of a leading sequence obtained. The sequence of the RT-PCR product is the same to that reported in the literature. The expressed rhIL-17 in E. coli can induce 10- to 15-fold increase of IL-6 secretion by mouse fibroblast 3T3 cells. It is for the first time until now that hIL-17 messager has been detected in B cell. There may exist some potential relationship between hIL-17 and myeloma cells.
Department of Molecular Genetics, Institute of Basic Medical Sciences, Beijing, People's Republic of China.
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L
Zhou
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S
Peng
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J
Duan
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J
Zhou
L
L
Wang
J
J
Wang
D016475:3T3 Cells; D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism*; D003001:Cloning, Molecular; D004926:Escherichia coli; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology
D020381:Interleukin-17; D015850:Interleukin-6; D011994:Recombinant Proteins