Stationary crystal diffraction with a monochromatic convergent X-ray source and application for macromolecular crystal data collection.
A diffraction geometry utilizing convergent X-rays from a polycapillary optic incident on a stationary crystal is described. A mathematical simulation of the resulting diffraction pattern (in terms of spot shape, position and intensity) is presented along with preliminary experimental results recorded from a lysozyme crystal. The effective source coverage factor is introduced to bring the reflection intensities onto the same scale. The feasibility of its application to macromolecular crystal data collection is discussed.
Ho
J X
JX
ES 76 Marshall Space Flight Center, NASA, Huntsville, Alabama 35812, USA. efe@zenith.umdnj.edu
Snell
E H
EH
Sisk
R C
RC
Ruble
J R
JR
Carter
D C
DC
Owens
S M
SM
Gibson
W M
WM
D000465:Algorithms; D000818:Animals; D002645:Chickens; D003625:Data Collection; D003627:Data Interpretation, Statistical; D046911:Macromolecular Substances; D009113:Muramidase / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods*
D046911:Macromolecular Substances; D009113:Muramidase