Fine mapping of 12 previously unassigned EST clones to individual YACs in the familial Alzheimer's disease (FAD) region of chromosome 14q24.3.
We have constructed an approximately 4-Mb YAC contig spanning the microsatellite markers D14S1028-D14S61 on chromosome 14q24.3 using oligonucleotide primers to microsatellite markers, ESTs and the genes PSEN1, FOS, TGFB3 and UBE2L1. This contig consists of 44 ICI YACs and 5 megaCEPH YACs. The data reported here should aid the final construction of an integrated map of chromosome 14 and allow the identification and accurate mapping of additional novel ESTs within this region.
Ardley
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HC
Molecular Medicine Unit, University of Leeds, St. James's University Hospital, Leeds, UK.
Markham
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Robinson
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D000544:Alzheimer Disease / Q000235:genetics*; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast; D002883:Chromosomes, Human, Pair 14; D003001:Cloning, Molecular; D004276:DNA, Satellite / Q000235:genetics; D005819:Genetic Markers; D006801:Humans; D011828:Radiation Chimera
D004276:DNA, Satellite; D005819:Genetic Markers