Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli.
The primary receptor (PotF) of the putrescine transport system in E. coli has been crystallized by the hanging-drop vapor-diffusion technique. The crystals belong to the space group P21212 with unit-cell dimensions a = 269.4, b = 82.33 and c = 93.74 A. The crystals diffract beyond 2.2 A with a rotating-anode X-ray source. A complete data set from the native crystals has been collected and processed at 2.3 A resolution. Two heavy-atom derivatives have been prepared from the same Pt compound at 293 and 277 K. The difference Patterson maps revealed completely different major heavy-atom sites between these two derivatives.
Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan. dmitry@crl.mei.co.jp
D G
DG
Vassylyev
T
T
Kashiwagi
H
H
Tomitori
K
K
Kashiwagi
K
K
Igarashi
K
K
Morikawa
D001692:Biological Transport; D003460:Crystallization; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins; D033902:Periplasmic Binding Proteins; D011700:Putrescine / Q000737:chemistry / Q000493:pharmacokinetics*; D017982:Receptors, Biogenic Amine / Q000737:chemistry*
D029968:Escherichia coli Proteins; D033902:Periplasmic Binding Proteins; D017982:Receptors, Biogenic Amine; C049683:potF protein, E coli; D011700:Putrescine