Separation of acetic and propionic acid analogs of L-thyroxine and L-triiodothyronine by thin-layer chromatography.
A thin-layer chromatographic method is described for the separation of the acetic and propionic acid analogs of thyroxine and triiodothyronine, which are inseparable by currently available methods. The separation is achieved on silica gel H in a solvent system consisting of methylacetate-2.5% ammonia (w/v) (95:5 v/v). The complete analysis takes 2 h.
Naidoo
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Department of Physiology-Anatomy, University of California, Berkeley 94720, USA.
D019342:Acetic Acid; D002855:Chromatography, Thin Layer / Q000379:methods*; D011422:Propionates; D013974:Thyroxine / Q000031:analogs & derivatives* / Q000302:isolation & purification; D014284:Triiodothyronine / Q000031:analogs & derivatives* / Q000302:isolation & purification
D011422:Propionates; D014284:Triiodothyronine; C010642:3,3',5-triiodothyroacetic acid; C012080:3,3',5-triiodothyropropionic acid; C029658:propionic acid; D019342:Acetic Acid; D013974:Thyroxine