Measurement of S-adenosyl-L-methionine levels by SP Sephadex chromatography.
Cation-exchange chromatography with sulfopropyl Sephadex was used to measure the levels of S-[methyl-3H]adenosyl-L-methionine synthesized by L1210 cells in vitro. Separation of S-[methyl-3H]adenosyl-L-methionine from [methyl-3H]methionine and other metabolites was achieved by stepwise elution with varying concentrations of HCl.
Laboratory of Medicinal Chemistry and Biology, National Cancer Institute, Bethesda, Maryland 20014, USA.
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RI
Glazer
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Peale
D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods*; D066298:In Vitro Techniques; D007939:Leukemia L1210 / Q000378:metabolism; D051379:Mice; D012435:S-Adenosylhomocysteine / Q000302:isolation & purification; D012436:S-Adenosylmethionine / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification
D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine