Gene encoding a human testis Sertoli cell component related to LIM domain protein.
The cDNA (HED-2) encoding a 20 kDa protein found in mammalian epididymal fluid was isolated from a human testis expression library. It is composed of 1908 bp, containing a reading frame of 1479 bp, coding a polypeptide consisting of 493 amino acids, and assigned the accession number: U15158 by GenBank (Biochem. Mol. Biol. Int. 34, 1131-1136, 1994). HED-2 has 99% identity with the zyxin gene in amino acid sequence, a component of cell junction matrix and a member of the LIM domain protein family. Northern blot analysis of RNAs prepared from various human tissues showed that the HED-2 gene was expressed in all tissues analyzed. Sertoli cells of human testis expressed the gene as determined by an in situ hybridization method. The present study shows that the HED-2 gene is a member of the LIM domain protein family.
Yang
J X
JX
National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Miao
S Y
SY
Wu
Y W
YW
Zhang
Z
Z
Zong
S D
SD
Wang
L F
LF
Koide
S S
SS
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics*; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D008667:Metalloproteins / Q000737:chemistry; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012708:Sertoli Cells / Q000737:chemistry*; D060589:Zyxin
D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D006023:Glycoproteins; D008667:Metalloproteins; D012333:RNA, Messenger; C092842:ZYX protein, human; D060589:Zyxin