Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family.
A new member of the staphylococcal bi-component leucotoxins family, LukE (32 kDa) and LukD (34.3 kDa) has been characterized from Staphylococcus aureus strain Newman. LukE was 58-68% identical with the class S proteins, whereas LukD was 71-77% identical with the class F proteins of the family. A partial immunoreactivity with the various affinity-purified antibodies specific for the other proteins was observed. Immunoprecipitation assay and gene probing confirmed a 30% frequency among human clinical isolates, differing from the distribution of the other known leucotoxins (P<0.005). LukE+LukD was as effective as the Panton-Valentine leucocidin for inducing dermonecrosis when injected in the rabbit skin, but not hemolytic and poorly leucotoxic compared to other leucotoxins expressed by Staphylococcus aureus.
UPRES EA-1318, Institut de Bactériologie de la Faculté de Médecine, Université Louis Pasteur-Hôpitaux Universitaires de Strasbourg, France.
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D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D001483:Base Sequence; D003001:Cloning, Molecular; D005098:Exotoxins / Q000737:chemistry / Q000235:genetics* / Q000633:toxicity*; D006801:Humans; D005779:Immunodiffusion; D008969:Molecular Sequence Data; D009336:Necrosis; D009504:Neutrophils / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry / Q000633:toxicity; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; C115218:LukD protein, Staphylococcus aureus; C115217:LukE protein, Staphylococcus aureus; D012333:RNA, Messenger; D011994:Recombinant Proteins