NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus.
N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of beta-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results were obtained using a dominant-negative NSF which is known to compete with endogenous NSF. The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus.
School of Life Science, Tokyo University of Pharmacy and Life Science, Hachioji, Japan.
T
T
Fukunaga
A
A
Furuno
K
K
Hatsuzawa
K
K
Tani
A
A
Yamamoto
M
M
Tagaya
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D020126:Brefeldin A; D016466:CHO Cells; D002352:Carrier Proteins / Q000378:metabolism*; D006224:Cricetinae; D003517:Cyclopentanes / Q000494:pharmacology*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure*; D018942:Macrolides; D008854:Microscopy, Electron; D052066:N-Ethylmaleimide-Sensitive Proteins; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D033921:Vesicular Transport Proteins
D000900:Anti-Bacterial Agents; D002352:Carrier Proteins; D003517:Cyclopentanes; D018942:Macrolides; D011500:Protein Synthesis Inhibitors; D033921:Vesicular Transport Proteins; D020126:Brefeldin A; D052066:N-Ethylmaleimide-Sensitive Proteins