Photosensitization of skin-derived cell lines by Dimegin [2,4-di-(alpha-methoxyethyl)-deuteroporphyrin IX] in vitro.
The deuteroporphyrin-IX derivative Dimegin [2,4-di-(alpha-methoxyethyl)-deuteroporphyrin IX] was investigated with respect to cellular uptake, intracellular localization and cell survival following photodynamic treatment in human cell lines derived from the skin (SCL1 and SCL2, squamous cell carcinoma; HaCaT keratinocytes; N1 fibroblasts). Using flow cytometry, we determined the cellular fluorescence as a marker of the uptake of Dimegin after incubation for 24 h. The intracellular localization of Dimegin was analysed using fluorescence microscopy and co-staining with fluorescent dyes specific for cell organelles. Following irradiation with an incoherent light source (580-740 nm) using a light dose of 24 J/cm2, phototoxicity was determined by means of trypan blue dye exclusion, MTT assays and growth curves. The relative Dimegin fluorescence of the different cell lines declined as follows: SCL1 > HaCaT > N1 > SCL2. Intracellular localization of Dimegin was found in the mitochondria. For all cell lines Dimegin concentrations above 15 microM yielded a significant phototoxic effect. The EC50 for SCL1 cells was 8.9 +/- 2.0 microM Dimegin. The EC50 for the cell lines increased as follows: SCL1 < HaCaT < N1 < SCL2, thus correlating with the cellular fluorescence of Dimegin. The results of the MTT assay were confirmed by trypan blue dye exclusion assay and growth curves. In conclusion, the study shows that Dimegin is an effective photosensitizer with a rapid mechanism of action in vitro, resulting in an immediate loss of plasma membrane integrity following irradiation.
Fickweiler
S
S
Department of Dermatology, University of Regensburg, Germany.
Szeimies
R M
RM
Abels
C
C
Ponomarev
G V
GV
Hofstädter
F
F
Wolfbeis
O S
OS
Landthaler
M
M
D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology; D002460:Cell Line; D002470:Cell Survival; D003905:Deuteroporphyrins / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D008856:Microscopy, Fluorescence; D017319:Photosensitizing Agents / Q000493:pharmacokinetics / Q000494:pharmacology* / Q000633:toxicity; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured / Q000378:metabolism / Q000473:pathology
D003905:Deuteroporphyrins; D017319:Photosensitizing Agents; C115898:dimegin(2,4-di-methoxyethyl)-deuteroporphyrin IX