Effect of temperature on the optical properties of ex vivo human dermis and subdermis.
The effect of temperature on the optical properties of human dermis and subdermis as a function of near-infrared wavelength has been studied between 25 degrees C and 40 degrees C. Measurements were performed ex vivo on a total of nine skin samples taken from the abdomen of three individuals. The results show a reproducible effect of temperature on the transport scattering coefficient of dermis and subdermis. The relative change of the transport scattering coefficient showed an increase for dermis ((4.7+/-0.5) x 10(-3) degrees C(-1)) and a decrease for subdermis ((-1.4+/-0.28) x 10(-3) degrees C(-1)). Note that the magnitude of the temperature coefficient of scattering was greater for dermis than subdermis. A reproducible effect of temperature on the absorption coefficient could not be found within experimental errors. System reproducibility in transport scattering coefficient with repeated removal and repositioning of the same tissue sample at the same temperature was excellent at +/-0.35% for all measurements. This reproducibility enabled such small changes in scattering coefficient to be detected.
University College London, Department of Medical Physics and Bioengineering, UK.
J
J
Laufer
R
R
Simpson
M
M
Kohl
M
M
Essenpreis
M
M
Cope
D055592:Biophysical Phenomena; D001703:Biophysics; D020405:Dermis / Q000502:physiology* / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D007259:Infrared Rays; D055095:Optics and Photonics / Q000295:instrumentation; D012542:Scattering, Radiation; D012879:Skin Physiological Phenomena; D012881:Skin Temperature / Q000502:physiology; D013055:Spectrophotometry, Infrared