Expression of receptors for basic fibroblast growth factor on human periodontal ligament cells.
Basic fibroblast growth factor (FGF-2; bFGF) is a major mitogen for connective tissue cells, and participates in the healing process. It has already been reported that FGF-2 could be applicable to enhance periodontal regeneration. In the present study, we examined FGF receptor (FGFR) expression on human periodontal ligament (PDL) cells. The binding of [125I]-labeled FGF-2 to human PDL cells was studied by radioreceptor assay. The binding of [125I]-FGF-2 to PDL cells reached a plateau after 2.5 h incubation at 4 degrees C and was inhibited by the addition of unlabeled FGF-2 and acidic FGF (FGF-1; aFGF), but not insulin-like growth factor-I, platelet-derived growth factor and transforming growth factor-beta 1. Scatchard analysis revealed the presence of approximately 1.0 x 10(5) FGF-2 binding sites per cell with an apparent Kd of 1.2 x 10(-10) M. Interestingly, the binding of [125I]-FGF-2 on PDL cells reached its maximum at d 6 of the culture and then gradually decreased. Scatchard analysis also demonstrated that the number of FGFRs on a PDL cell was altered during the course of the culture, while the affinity between FGF-2 and its receptor was not. The responsiveness of PDL cells to FGF-2, which was monitored by the inhibitory effect on alkaline phosphatase activity, was reduced in proportion to the decrease in the number of FGFRs on the PDL cells. The present study suggests that PDL cells alter the responsiveness to FGF-2 during the course of the culture by changing the density of its receptor, and that the density of FGFR expression might be a marker of the cytodifferentiation of PDL cells into mineralized tissue forming cells.
Takayama
S
S
Department of Periodontology and Endodontology, Osaka University Faculty of Dentistry, Japan.
Murakami
S
S
Nozaki
T
T
Ikezawa
K
K
Miki
Y
Y
Asano
T
T
Terashima
A
A
Okada
H
H
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D016220:Fibroblast Growth Factor 1 / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000032:analysis* / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology; D007457:Iodine Radioisotopes; D010513:Periodontal Ligament / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D019275:Radiopharmaceuticals; D017468:Receptors, Fibroblast Growth Factor / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000235:genetics; D012038:Regeneration; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D004791:Enzyme Inhibitors; D007457:Iodine Radioisotopes; D010982:Platelet-Derived Growth Factor; D019275:Radiopharmaceuticals; D017468:Receptors, Fibroblast Growth Factor; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D007334:Insulin-Like Growth Factor I; D000469:Alkaline Phosphatase