Comparison of growth and metabolism of avian osteoblasts on polished disks versus thin films of titanium alloy.
The purpose of this study was to evaluate the efficacy of using high vacuum, thermal evaporation to deposit thin films of Ti-6Al-4V onto plates for subsequent cell culture investigations. Osteoblastic response to thin-film coated plates was compared to that of cells grown on Ti alloy disk inserts and uncoated culture plates. The Ti alloy disks were polished, cleaned, and passivated following a commercial protocol for orthopedic implants. Mean surface roughness was 262 nm for the Ti alloy disks and 4.756 nm for the coated culture plates. Osteoblasts isolated from 16-day chick embryo calvariae were cultured on polystyrene, thin films, and disks. At confluence, the cells were cultured an additional 48 h and were evaluated for cell number (DNA content), rate of glycolysis (lactate production), alkaline phosphatase activity (ALPase), and collagenous (3H-proline hydroxylation) and noncollagenous protein synthesis. Cell morphology was similar for the controls, disks, and thin-film groups. DNA, lactate, cell layer ALPase, 3H-hydroxyproline, and noncollagenous protein were not different (p > 0.05) among the control, thin-film, and disk groups. Medium ALPase was lower (p < 0.05) in the thin-film group compared to the control group. Although aluminum and vanadium percentages varied from nominal in the thin-film groups (11Al-2V as opposed to 6Al-4V), avian osteoblasts responded similarly to the Ti alloy thin films, disks, and uncoated culture plates for the smooth surfaces tested. The thin-film cell culture system used for elemental material studies appears to offer a promising method for the investigation of cellular response to alloyed biomaterials as well. Proper adjustments in alloy percentages before deposition, however, need to be made if thermal evaporation is utilized.
Orthopaedic Research Laboratories, Carolinas Medical Center, Charlotte, North Carolina 28232, USA.
C S
CS
Koontz
W K
WK
Ramp
R D
RD
Peindl
K K
KK
Kaysinger
M E
ME
Harrow
D000818:Animals; D001672:Biocompatible Materials; D018929:Cell Culture Techniques / Q000379:methods; D002455:Cell Division; D002642:Chick Embryo; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism*; D014025:Titanium
D001672:Biocompatible Materials; D014025:Titanium