Determination of naringenin and its glucuronide conjugate in rat plasma and brain tissue by high-performance liquid chromatography.
An isocratic high-performance liquid chromatographic method with ultraviolet detection was utilized for the investigation of the pharmacokinetics of naringenin and its glucuronide conjugate in rat plasma and brain tissue. Plasma and brain tissue were deproteinized by acetonitrile, then centrifuged for sample clean-up. The drugs were separated by a reversed-phase C18 column with a mobile phase consisting of acetonitrile-orthophosphoric acid solution (pH 2.5-2.8) (36:64, v/v). The detection limits of naringenin in rat plasma and brain tissue were 50 ng/ml and 0.4 microg/g, respectively. The glucuronide conjugate of naringenin was evaluated by the deconjugated enzyme beta-glucuronidase. The naringenin conjugation ratios in rat plasma and brain tissue were 0.86 and 0.22, respectively, 10 min after naringenin (20 mg/kg, i.v.) administration. The mean naringenin conjugation ratio in plasma was approximately four fold that in brain tissue.
Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan.
H W
HW
Peng
F C
FC
Cheng
Y T
YT
Huang
C F
CF
Chen
T H
TH
Tsai
D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044950:Flavanones; D005419:Flavonoids / Q000032:analysis* / Q000097:blood; D005965:Glucuronates / Q000032:analysis / Q000097:blood; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D044950:Flavanones; D005419:Flavonoids; D005965:Glucuronates; C005273:naringenin