Crystallization of the alpha-hemolysin heptamer solubilized in decyldimethyl- and decyldiethylphosphine oxide.
Crystals of the alpha-hemolysin heptamer, a transmembrane pore-forming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 A resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 A, alpha = 92.6, beta = 94.8, gamma = 90.2 degrees.
Song
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Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.
Gouaux
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D001427:Bacterial Toxins / Q000737:chemistry* / Q000302:isolation & purification*; D003460:Crystallization; D003902:Detergents; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D010720:Phosphines; D011487:Protein Conformation; D012995:Solubility; D013211:Staphylococcus aureus / Q000737:chemistry; D014961:X-Ray Diffraction
C091451:APO 10; D001427:Bacterial Toxins; D003902:Detergents; D006460:Hemolysin Proteins; D010720:Phosphines; C115338:decyldiethylphosphine oxide; C021091:staphylococcal alpha-toxin