[Platelet calcium oscillation by laser scanning confocal microscopy].
OBJECTIVE
To observe platelet calcium oscillation and the influence of extracellular Ca2+ on it.


METHODS
We observed a single platelet intracellular Ca++ change and the influence of extracellular Ca2+ on it by laser sanning confocal microscopy when various agonists of different concentrations stimulated platelets.


RESULTS
The other agonists except thrombin induced platelet calcium oscillation. With increasing agonis concentrations, the frequence of calcium oscillation enhanced and calcium oscillation sustained longer. In the absence of Ca++, the amplitude of ADP-induced calcium oscillation was smaller without frequence variations, and 5-TH induced calcium oscillation was inhibited.


CONCLUSIONS
Platelet calcium oscillation increased from fluctuation either in the entry of external calcium or the release from internal stores.
General Hospital of PLA, Beijing.
Y
Y
Cong
J
J
Wang
N
N
Li
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001693:Biological Transport, Active; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006801:Humans; D018613:Microscopy, Confocal; D012701:Serotonin / Q000494:pharmacology
D012701:Serotonin; D000244:Adenosine Diphosphate; D002118:Calcium