Structure refinement against synchrotron Laue data: strategies for data collection and reduction.
The synchrotron Laue technique has been applied to high-resolution structure refinement of the ribotoxin, restrictocin [Yang & Moffat (1996). Structure, 4, 837-852]. By employing carefully designed data-collection strategies and the data-reduction algorithms incorporated in the software system LaueView [Ren & Moffat (1995a). J. Appl. Cryst. 28, 461-481; Ren & Moffat (1995b). J. Appl. Cryst. 28, 482-493], a set of high-resolution Laue data with a completeness and accuracy comparable to excellent monochromatic data was obtained. Through detailed comparison with the monochromatic data and electron-density maps derived from the Laue data, optimum data-collection and reduction strategies were identified and the application of Laue diffraction techniques to conventional crystallographic refinement was demonstrated.
Yang
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X
Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA.
Ren
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Z
Moffat
K
K
D000465:Algorithms; D018360:Crystallography, X-Ray / Q000379:methods*; D003625:Data Collection; D003627:Data Interpretation, Statistical; D008958:Models, Molecular; D008969:Molecular Sequence Data; D015394:Molecular Structure; D017356:Synchrotrons