Cloning and sequence determination of a phi AAU2 gene whose product aborts the phage lytic cycle.
This communication describes the cloning of a 1.8-kb fragment from the genome of the corynephage phi AAU2, which aborts the phage lytic cycle when cloned on a high-copy shuttle vector. The associated phenotype, called Apld (aborting phage lytic development), was revealed by noting the reduced plaque size and lower efficiencies of plaquing of phi AAU2 cp, a virulent derivative of phi AAU2, on "Arthrobacter aureus"-C70 Apld+ cells. Adsorption and phage DNA transfection experiments showed evidence that Apld acted once the phage DNA had entered into the cell; apld was confined to a single open reading frame (ORF), encoding a putative 63-aa polypeptide which did not show any homology to proteins contained in the databanks; apld is followed by an ORF the product of which shows homology with a protein expressed by the early region of the Streptomyces phage phi C31.
Le Marrec
C
C
Membranes et Osmorégulation, UPRES-A 6026, Université de Rennes I, France.
Moreau
S
S
Leret
V
V
Schäffer
A
A
Blanco
C
C
Trautwetter
A
A
D000595:Amino Acid Sequence; D001173:Arthrobacter / Q000737:chemistry / Q000235:genetics*; D001435:Bacteriophages / Q000737:chemistry* / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000737:chemistry; D008242:Lysogeny; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D016366:Open Reading Frames; D015183:Restriction Mapping; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014162:Transfection; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D014774:Virulence
C115330:Apld protein, Corynephage phiAAU2; D004269:DNA, Bacterial; D014764:Viral Proteins