[Comparison between 2 monoclonal antibodies (clones 95/12 and 1C3 + AYm-1) for the detection of pp65 antigenemia associated with human cytomegalovirus].
BACKGROUND
A prospective parallel and blind comparative study was carried out to evaluate the diagnostic efficacy of two available anti-pp65 monoclonal antibodies (clone 95/12 and the pool 1C3 + AYM-1) for the cytomegalovirus (CMV) antigenemia assay.


METHODS
We carried out a comparative study of 107 blood samples from immunodepressed patients (renal transplant and AIDS patients) with suspected disseminated infection by CMV. The PMNLs were obtained using the method of sedimentation in saline dextran. Slides were stained by an indirect immunofluorescence assay with two commercially available monoclonal antibodies.


RESULTS
Of the 107 blood samples studied 33 (30.8%) had a positive antigenemia test. The clone 95/12 detected 30 (90.9%) samples and the pool 31 (93.9%), no statistically significant difference was observed in the sensitivity of two reagents (p = 0.42). The values of the mean CMV-positive cell count obtained with the clone 95/12 was 60.6 vs 61.9 with the monoclonal pool (p = 0.026).


CONCLUSIONS
No significant difference was detected between the two commercial monoclonal antibodies. However the pool detected a slightly superior CMV-positive cell count.
Unidad de Virología, Servicio de Microbiología Clínica, Hospital Universitario Son Dureta, Palma de Mallorca.
J
J
Reina
M
M
Munar
I
I
Blanco
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000276:immunology; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016867:Immunocompromised Host; D016030:Kidney Transplantation; D009504:Neutrophils / Q000821:virology; D010750:Phosphoproteins / Q000097:blood* / Q000276:immunology; D011183:Postoperative Complications / Q000175:diagnosis / Q000276:immunology / Q000821:virology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D016037:Single-Blind Method; D014763:Viral Matrix Proteins / Q000097:blood* / Q000276:immunology; D014766:Viremia / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D010750:Phosphoproteins; D014763:Viral Matrix Proteins; C065418:cytomegalovirus matrix protein 65kDa
Comparación entre dos anticuerpos monoclonales (clones 95/12 y 1C3 + AYM-1) en la detección de antigenemia pp65 frente al citomegalovirus humano.