Endogenous inhibitors of nitric oxide synthesis and lipid peroxidation in hyperlipidemic rabbits.
OBJECTIVE
To examine whether the elevation of endogenous NG, NG-dimethylarginine (DMA) content is related to lipid peroxidation in the high lipid-fed rabbit.


METHODS
In high lipid diet-fed rabbits, concentrations of serum cholesterol, triglyceride, malondialdehyde (MDA), and DMA were measured, and endothelium-dependent relaxation to acetylcholine (ACh) was tested.


RESULTS
After 6-wk on a high lipid-diet, the levels of serum total cholesterol, triglyceride, MDA, and DMA were increased vs those in control group (MDA was 2.88 +/- s 0.20 vs 1.54 +/- 0.13 nmol.L-1, P < 0.01 and DMA was 1.51 +/- 0.07 vs 0.75 +/- 0.13 mumol.L-1, P < 0.01), while the endothelium-dependent vasodilation in the isolated thoracic aorta was impaired (the maximal response to ACh was 45.59 +/- 3.10 vs 76.93 +/- 5.68%). Supplementation with vitamin E decreased MDA and DMA content and improved the endothelium-dependent vasodilation.


CONCLUSIONS
An increase in serum concentration of DMA may be secondary to the elevation of lipid peroxides in the hyperlipidemic rabbit.
Department of Pharmacology, Hu-nan Medical University Changsha, China.
Y
Y
Xiong
Y J
YJ
Li
X J
XJ
Yu
G Z
GZ
Liu
N S
NS
Li
D000818:Animals; D001013:Aorta, Thoracic; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D006949:Hyperlipidemias / Q000097:blood*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D009569:Nitric Oxide / Q000096:biosynthesis; D011817:Rabbits; D014664:Vasodilation / Q000187:drug effects; D014810:Vitamin E / Q000494:pharmacology
D014810:Vitamin E; D009569:Nitric Oxide; D008315:Malondialdehyde; C018524:N,N-dimethylarginine; D001120:Arginine