Structural model of phospholipid-reconstituted human transferrin receptor derived by electron microscopy.
BACKGROUND
The transferrin receptor (TfR) regulates the cellular uptake of serum iron. Although the TfR serves as a model system for endocytosis receptors, neither crystal structure analysis nor electron microscopy has yet revealed the molecular dimensions of the TfR. To derive the first molecular model, we analyzed purified, lipid-reconstituted human TfR by high-resolution electron microscopy.


RESULTS
A structural model of phospholipid-reconstituted TfR was derived from 72 cryo-electron microscopic images. The TfR dimer consists of a large extracellular globular domain (6.4 x 7.5 x 10.5 nm) separated from the membrane by a thin molecular stalk (2.9 nm). A comparative protein sequence analysis suggests that the stalk corresponds to amino acid residues 89-126. Under phospholipid-reconstitution conditions, the human TfR not only integrates into vesicles, but also forms rosette-like structures called proteoparticles. Scanning transmission electron microscopy revealed an overall diameter of 31.5 nm and a molecular mass of 1669 +/- 26 kDa for the proteoparticles, corresponding to nine TfR dimers. The average mass of a single receptor dimer was determined as being 186 +/- 4 kDa.


CONCLUSIONS
Proteoparticles resemble TfR exosomes that are expelled by sheep reticulocytes upon maturation. The structure of proteoparticles in vitro is thus interpreted as being the result of the TfR's strong self-association potential, which might facilitate the endosomal sequestration of the TfR away from other membrane proteins and its subsequent return to the cell surface within tubular structures. The stalk is assumed to facilitate the tight packing of receptor molecules in coated pits and recycling tubuli.
Institut für Laboratoriumsmedizin und Pathobiochemie Charité Campus Virchow-Klinikum Augustenburger Platz 1, D-13353, Berlin, Germany.
H
H
Fuchs
U
U
Lücken
R
R
Tauber
A
A
Engel
R
R
Gessner
D000818:Animals; D002645:Chickens; D006224:Cricetinae; D020285:Cryoelectron Microscopy; D006801:Humans; D051379:Mice; D017348:Microscopy, Electron, Scanning Transmission; D008961:Models, Structural; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*; D011990:Receptors, Transferrin / Q000737:chemistry / Q000648:ultrastructure*
D010713:Phosphatidylcholines; D010743:Phospholipids; D011990:Receptors, Transferrin