Multiple restricted origin of oligodendrocytes.
The plp gene encodes the proteolipid protein and its alternatively spliced product DM-20, major proteins of CNS myelin. In the mouse, plp/dm-20 transcripts are expressed beginning at embryonic day 9.5 (E9.5) by restricted foci of germinative neuroepithelial cells. To determine the identity of the neural precursors expressing plp/dm- 20, a zeomycin resistance gene fused to the lacZ reporter was expressed in transgenic mice under the control of the plp regulatory sequences. In the three different lines generated, the pattern of beta-galactosidase expression was similar and superimposable on the expression pattern of endogenous plp/dm-20. Both in vivo and in vitro, the transgene was expressed by O4(+) pre-oligodendrocytes, and later by RIP+ differentiated oligodendrocytes, but not by neuronal cells, astrocytes, or radial glial cells. After zeomycin selection, a dramatic enrichment in O4(+) pre-oligodendrocytes was observed in cultures derived from E12.5 transgenic embryos. This enrichment indicates the oligodendroglial specification of neural precursors that continuously express plp/dm-20. Early plp/dm-20-expressing precursors, however, appear to be a separate population from previously described PDGFRalpha oligodendrocyte precursors, as shown by the striking differences in their (1) patterns of distribution and (2) responsiveness to PDGF. These data suggest that oligodendrocytes have a plural origin and that early plp/dm-20 defines one of the neural lineages generating oligodendrocytes.
Spassky
N
N
Biologie des Interactions Neurones/Glie, Institut National de la Santé et de la Recherche Médicale U-495, Université Pierre Marie Curie, Hôpital de la Salpêtrière, 75651 Paris Cedex 13, France.
Goujet-Zalc
C
C
Parmantier
E
E
Olivier
C
C
Martinez
S
S
Ivanova
A
A
Ikenaka
K
K
Macklin
W
W
Cerruti
I
I
Zalc
B
B
Thomas
J L
JL
D000818:Animals; D015415:Biomarkers; D001761:Bleomycin / Q000494:pharmacology; D001923:Brain Chemistry / Q000235:genetics; D002454:Cell Differentiation / Q000502:physiology; D019070:Cell Lineage / Q000502:physiology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000196:embryology; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D004352:Drug Resistance, Microbial; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007763:Lac Operon; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D009836:Oligodendroglia / Q000737:chemistry / Q000166:cytology* / Q000502:physiology*; D017479:Receptors, Platelet-Derived Growth Factor / Q000032:analysis / Q000235:genetics; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D014157:Transcription Factors / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes / Q000502:physiology; D001616:beta-Galactosidase / Q000235:genetics
D015415:Biomarkers; D004268:DNA-Binding Proteins; C489726:Myt1 protein, mouse; D014157:Transcription Factors; D001761:Bleomycin; C105427:Zeocin; D017479:Receptors, Platelet-Derived Growth Factor; D001616:beta-Galactosidase