[Oxygen free radical on interleukin-1 activity of hemorrhage/resuscitation rat].
OBJECTIVE
To investigate the effects of oxygen free radical on the enhancement of IL-1 activity in vivo and in vitro on hemorrhagic and resuscitated rat.


METHODS
30% of rats total blood volume was withdraw by carotid artery catheter and resuscitated with 2 times of lost blood volume 1 h later.


RESULTS
Plasm IL-1 activity and MDA content increased and SOD activity decreased significantly 1-4 hours after resuscitation. There was a marked correlation between IL-1 activity and MDA content as well as SOD activity. Treatment with SOD as resuscitation prevented the postresuscitation increase in plasma IL-1 activity significantly. Hemorrhage and resuscitation also caused significant decrease of SOD activity and elevation of MDA in peritoneal macrophage 2 hours after resuscitation. After preincubation with SOD for 1 hour, the macrophage presented a much lowered capacity to release IL-1.


CONCLUSIONS
Oxygen free radical may be one of the most important factors that contribute to elevation of IL-1 level after hemorrhage and resucitation.
Department of General Surgery, South Hospital, Guangzhou.
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D000818:Animals; D005260:Female; D005609:Free Radicals; D006470:Hemorrhage / Q000378:metabolism*; D007375:Interleukin-1 / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism; D012151:Resuscitation; D013482:Superoxide Dismutase / Q000378:metabolism*
D005609:Free Radicals; D007375:Interleukin-1; D008315:Malondialdehyde; D013482:Superoxide Dismutase