Purification of small peptides labeled with Bolton-Hunter reagent.
A method utilizing high-voltage electrophoresis on paper is described whereby a pentapeptide (Asp-Ser-Asp-Pro-Arg) labeled with Bolton-Hunter reagent is separated from hydrolyzed reagent and unreacted peptide and is recovered from the electrophoretogram in high yield. The general applicability to other peptides is discussed.
Department of Biochemistry and Drug Disposition, USV Pharmaceutical Corporation, Tuckahoe, New York 10707, USA.
S M
SM
Coutts
D M
DM
Reid
D004590:Electrophoresis, Paper / Q000379:methods; D006801:Humans; D007073:Immunoglobulin E / Q000302:isolation & purification; D007202:Indicators and Reagents; D010446:Peptide Fragments / Q000302:isolation & purification; D010455:Peptides / Q000302:isolation & purification*; D013388:Succinimides
D007202:Indicators and Reagents; D010446:Peptide Fragments; D010455:Peptides; D013388:Succinimides; D007073:Immunoglobulin E; C022581:Bolton-Hunter reagent; C040297:pentigide