Detection and typing of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks attached to human skin by PCR.
Live Ixodes ricinus ticks attached to humans residing in Germany were examined for borreliae by dark-field microscopy and PCR. Borrelia species were identified by 16S rRNA sequence analysis, which showed the presence of several species, some not yet defined, and a high prevalence of multiply infected ticks.
Liebisch
G
G
Institute of Medical Microbiology, Hannover Medical School, Hannover, Germany.
Sohns
B
B
Bautsch
W
W
D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D015748:Borrelia burgdorferi Group / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017931:DNA Primers / Q000235:genetics; D005069:Evaluation Studies as Topic; D005858:Germany / Q000453:epidemiology; D006801:Humans; D018884:Ixodes / Q000382:microbiology*; D008193:Lyme Disease / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D012867:Skin / Q000382:microbiology* / Q000469:parasitology*; D013045:Species Specificity
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S