Interaction of antibody with antigen immobilized on polystyrene latex beads: characterization by density gradient centrifugation.
Isopycnic banding by density gradient centrifugation was used to measure density changes in complexes formed by the interaction between antigen and antibody immobilized on polystyrene latex beads (diameter, 0.109 +/- 0.0025 micron). Measurements of density changes allowed calculation of the interacting masses under the given experimental conditions. Interaction equilibrium constants and free energy change for two sets of reactions, bovine IgG and anti-bovine IgG (rabbit) IgG and rabbit IgG and anti-rabbit IgG (goat) IgG systems, were calculated from isopycnic banding density gradient centrifugation runs. The procedure demonstrates a new method of obtaining quantitative information on antigen-antibody interactions.
Department of Chemical, Metallurgical, and Polymer Engineering, University of Tennessee, Knoxville 37916, USA.
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Genung
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Hsu
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D002417:Cattle; D002500:Centrifugation, Isopycnic / Q000379:methods; D007074:Immunoglobulin G / Q000302:isolation & purification; D008863:Microspheres; D011137:Polystyrenes; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D011137:Polystyrenes