Sequencing of a gene encoding a member of the mitochondrial carrier family of transport proteins from Aspergillus nidulans.
Mitochondrial carrier proteins comprise a superfamily of evolutionarily conserved proteins that regulate the specific transport of essential metabolites across the mitochondrial membranes. In this report we describe the cloning and sequencing of a gene from Aspergillus nidulans, amc-1, that encodes the first reported example of a mitochondrial carrier protein in Aspergillus species. The amc-1 gene is located on chromosome 7, and is transcribed as a 1.6 kb unspliced polyadenylated RNA. The predicted translation product of the amc-1. cDNA displays three tandemly repeated domains which possess protein signature motifs that are characteristic of mitochondrial carrier proteins that localize to the inner mitochondrial membrane. amc-1 shares the greatest similarity with a Neurospora mitochondrial carrier protein that is implicated in basic amino acid transport, suggesting that the amc-1 protein may provide a related function.
Department of Pathology & Laboratory Medicine, University of Cincinnati, OH 45267-0529, USA.
J
J
Sun
J C
JC
Rhodes
D S
DS
Askew
D000595:Amino Acid Sequence; D026923:Amino Acid Transport Systems, Basic; D001233:Aspergillus nidulans / Q000235:genetics*; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D015698:Genomic Library; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
C116043:Amc-1 protein, Aspergillus nidulans; D026923:Amino Acid Transport Systems, Basic; D002352:Carrier Proteins; D018076:DNA, Complementary; D005656:Fungal Proteins