Staphylococcal protein A-sepharose columns and the characterization of measles virus-specific polypeptides in persistently infected cells.
Viral polypeptides in extracts prepared from [35S]methionine-labeled human epithelial carcinoma cells persistently infected with measles virus were reacted with virus-specific antisera. Microcolumns of staphylococcal protein A linked to Sepharose were used to isolate antigen-antibody complexes that contained few contaminating host polypeptides. Measles virus polypeptides in these complexes could be identified readily on sodium dodecyl sulfate-polyacrylamide gels even when antiserum with low reactivity toward the viral antigens was used.
Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710, USA.
E C
EC
Hayes
L L
LL
Wright
H J
HJ
Zweerink
D000818:Animals; D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D002847:Chromatography, Agarose / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D008459:Measles virus / Q000737:chemistry* / Q000276:immunology; D012685:Sepharose / Q000031:analogs & derivatives; D014764:Viral Proteins / Q000276:immunology / Q000302:isolation & purification*; D013205:Staphylococcal Protein A
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D012685:Sepharose; D014764:Viral Proteins; C042030:Staphylococcal protein A-sepharose; D013205:Staphylococcal Protein A