Crystallization of succinylated concanavalin A bound to a synthetic bivalent ligand and preliminary structural analysis.
Crystals have been obtained of succinylated concanavalin A complexed to a novel bidentate synthetic ligand. The crystals are the first example of a lectin with a synthetic multivalent ligand and the first report of crystallization of succinylated concanavalin A. The crystals were obtained by sitting-drop vapour diffusion equilibrating with a solution of 20% polyethylene glycol, pH 5, 293. 5 K. Crystals are orthorhombic, belonging to space group C2221 with unit-cell dimensions of a = 99.1, b = 127.4, c = 118.9 A. The asymmetric unit contains a dimer, with over 65% of the volume occupied by water. The ligand cross links concanavalin A monomers. Succinylated concanavalin A is known to be a dimer in solution, yet it is found as the typical concanavalin A tetramer in the crystal. The contacts holding together the tetramer appear extensive and suggest that a fine balance between dimer and tetramers exists. Data to 2.65 A have been collected and the structure determined by the molecular replacement method.
Centre for Biomolecular Science, Purdie Building, The University, St Andrews KY16 9ST, Scotland.
D N
DN
Moothoo
S A
SA
McMahon
S M
SM
Dimick
E J
EJ
Toone
J H
JH
Naismith
D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D003208:Concanavalin A / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D003460:Crystallization; D018360:Crystallography, X-Ray; D008024:Ligands; D046911:Macromolecular Substances; D008362:Mannosides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D011485:Protein Binding; D011487:Protein Conformation; D013386:Succinates / Q000737:chemistry
C115881:1,3-di-(N-propyloxymannopyranosyl)carbamoyl-5-methylazidobenzene; D001386:Azides; D008024:Ligands; D046911:Macromolecular Substances; D008362:Mannosides; D013386:Succinates; D003208:Concanavalin A