Effect of nicotine and cotinine on the susceptibility to in vitro oxidation of LDL in healthy non smokers and smokers.
Cigarette smoke of which the major component is nicotine plays an important role in the development of cardiovascular diseases. To study the effect of in vitro incubation of LDL with nicotine and its metabolite, cotinine on a copper-induced peroxidation, we monitored the formation of conjugated dienes, hydroperoxides and thiobarbituric acid-reactive substances production. The LDL studied were taken from six non-smokers (aged 41.5 years) and six smokers who consumed at least ten cigarettes per day (40.7 years). LDL oxidation with CuSO4 showed that cigarette smoking promotes LDL susceptibility to peroxidative modification. During the peroxidation of LDL with nicotine (O to 5 mmol/1) and CuSO4 (5 micromol/l), the formation of hydroperoxides decreased when nicotine concentrations increased and the production of TBARS increased in a concomitant manner. The results showed that the presence of nicotine destabilized the production of hydroperoxides in LDL and increased the formation of secondary oxidation products. On the other hand, cotinine had no effect on LDL oxidative susceptibility in smokers and non-smokers.
Laboratory of Chemical Pathology, Rangueil University Hospital, Toulouse, France. valdiguie@rangueil.inserm.fr
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Gouazé
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Dousset
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JC
Dousset
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Valdiguié
D000328:Adult; D019327:Copper Sulfate / Q000494:pharmacology; D003367:Cotinine / Q000378:metabolism / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood* / Q000378:metabolism; D009538:Nicotine / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012907:Smoking / Q000097:blood; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; D009538:Nicotine; D004492:Edetic Acid; D003367:Cotinine; D019327:Copper Sulfate