[Quantitative analysis of inducible systems of Escherichia coli and Bacillus subtilis using radioimmunological methods].
By RIA dot-blot method two main cell system, system SOS-repair and heat shock system, was showed possibility to receive as well qualitative as quantitative results influence of different induced agents. Was showed quantitative differences in initial levels enzymes of RecA and CroEL in different strains of E. coli, as well of principle possibility to using received antibody, which has high specific to this enzymes, for investigation reactions this systems in the other bacterial species, such as Bac. subtilis.
B.P. Konstantinov St.-Petersburg Institute of Nuclear Physics, Academy of Sciences of Russia, Gatchina.
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D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D000907:Antibodies, Bacterial; D001412:Bacillus subtilis / Q000378:metabolism* / Q000528:radiation effects; D018834:Chaperonin 60 / Q000276:immunology / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D018882:Heat Stress Disorders; D008937:Mitomycins / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods; D011938:Rec A Recombinases / Q000276:immunology / Q000378:metabolism*; D013014:SOS Response, Genetics
D000903:Antibiotics, Antineoplastic; D000907:Antibodies, Bacterial; D018834:Chaperonin 60; D008937:Mitomycins; D011938:Rec A Recombinases
Kolichestvennoe issledovanie indutsibel'nykh sistem kletok Escherichia coli i Bacillus subtilis radioimmunologicheskim metodom.