Enhancement of interleukin-2-induced lymphokine-activated killer activity by interleukin 7 against autologous human renal cell carcinoma.
Adjuvant immunotherapy with interferons and/or interleukin 2 (IL-2) is widely used for advanced kidney cancer. However, the results are not satisfactory so far. The purpose of this study is to evaluate the inducible activity of lymphokine-activated killer (LAK) cells against autologous human renal cell carcinoma. The effect of interleukin 7 (IL-7) on IL-2-induced LAK activity was assessed by the autologous assay system which we have established. Peripheral blood lymphocytes from patients with renal cell carcinoma were stimulated with IL-2 and/or IL-7, and tested for antitumor activity against autologous renal cell carcinoma. In all 10 cases tested, IL-7 alone induced LAK activity. Moreover, IL-2-induced LAK activity was augmented by the concomitant addition of IL-7. Flow cytometry revealed an increase in IL-2-receptor-positive lymphocytes following incubation with IL-7. These results suggest that combination therapy using IL-2 and IL-7 may be a useful treatment for patients with advanced renal cell carcinoma.
Department of Urology, Osaka University Medical School, Osaka, Japan.
M
M
Kondo
N
N
Nonomura
T
T
Miki
Y
Y
Kojima
M
M
Yokoyama
E
E
Nakano
A
A
Okuyama
D000368:Aged; D015703:Antigens, CD / Q000097:blood; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000628:therapy*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016219:Immunotherapy, Adoptive / Q000379:methods*; D007376:Interleukin-2 / Q000494:pharmacology*; D015851:Interleukin-7 / Q000494:pharmacology*; D007680:Kidney Neoplasms / Q000097:blood / Q000628:therapy*; D015979:Killer Cells, Lymphokine-Activated / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D008875:Middle Aged
D015703:Antigens, CD; D004273:DNA, Neoplasm; D007376:Interleukin-2; D015851:Interleukin-7