[Diagnosis of cytomegalovirus infections in patients with HIV infection. Comparison of the indirect immunofluorescence and viral isolation techniques].
The technique for the detection of fluorescent early antigen was compared to the classical method of viral isolation in cells from the human lung. The study was performed with the use of 85 urine samples from 64 patients presenting with HIV infection. The technique for the detection of fluorescent early antigens showed a sensitivity of 91%, an specificity of 97%, and a coincidence of 94% with respect to viral isolation. The main advantage of the technique for the detection of fluorescent early antigens with respect to viral isolation is that the former provides a quicker diagnosis of cytomegalovirus infection within 48-72 hours, besides being easy to perform.
Instituto de Medicina Tropical Pedro Kouri.
A
A
Valdivia
M
M
Alvarez
M
M
Marrero
O
O
Valdés
M
M
Muné
D000956:Antigens, Viral / Q000302:isolation & purification; D003587:Cytomegalovirus / Q000276:immunology / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D019084:Fluorescent Antibody Technique, Indirect; D015658:HIV Infections / Q000150:complications*; D006801:Humans; D012680:Sensitivity and Specificity
D000956:Antigens, Viral
Diagnóstico de la infección por Citomegalovirus en pacientes infectados por el VIH. Comparación de las técnicas de inmunofluorescencia indirecta y aislamiento viral.