Increased serum IgG1 levels and reduced numbers of B-1 B cells in DBA/2J mice.
B-cell heterogeneity studies have historically focused upon BALB/c mice and their derivatives. In contrast, the B cells of DBA/2J mice, a prototype strain for the study of the endogenous minor lymphocyte stimulatory (Mls) viral superantigen Mls-1a, have not been extensively investigated. DBA/2J B cells, by functioning as Mls-1a antigen-presenting cells, influence their own differentiation and diversity by inducing the proliferation and differentiation of specific CD4 T-cell subsets. In this report, the B cells of DBA/2J and BALB/c mice were compared for their ability to restore B-cell function in severe combined immunodeficient (SCID) recipients. Although spleen and bone marrow cells from these strains exhibited similar restoration of serum IgM production, the transfer of DBA/2J B cells into SCID mice led to greater IgG1 production. The peritoneal cells of DBA/2J mice consisted of a lower percentage of B-1 B cells and were less capable of restoring B-cell function after transfer into SCID recipients. These differences are discussed with respect to the possible role of viral superantigens in influencing B-lymphocyte diversity.
Department of Biology, Rider University, Lawrenceville, NJ 08648-3099, USA.
K
K
Tocce
G
G
Masters
C
C
Rago
L
L
Prior
K
K
Suppiah
T
T
Glysing-Jensen
S
S
Leary
J
J
Riggs
D019264:Adoptive Transfer; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D005434:Flow Cytometry; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA / Q000276:immunology*; D016513:Mice, SCID; D010537:Peritoneum / Q000276:immunology; D012190:Retroviridae / Q000276:immunology; D016511:Severe Combined Immunodeficiency / Q000276:immunology* / Q000628:therapy*; D018089:Superantigens / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D018089:Superantigens