Investigation of the cellular tropism of bovine immunodeficiency-like virus.
Bovine immunodeficiency-like virus (BIV) was first isolated from an animal showing transient leucocytosis, lymphadenopathy, lesions in the central nervous system and progressive weakness and emaciation. Similar signs are observed in other immunosuppressive lentiviral infections. BIV, like other lentiviruses, has been isolated from peripheral blood mononuclear cells and lymphoid tissue of infected animals. However, the in vivo cellular tropism of BIV remains unclear although initial studies indicate that BIV may be pantropic, infecting T cells, B cells and monocytes similar to some of the immunodeficiency-causing lentiviruses. PCR, Southern blot hybridisation, cell culture and reverse transcriptase assays were used to demonstrate the presence of BIV proviral DNA and the production of infectious virus in CD2+, WC1+, B cells and monocytes during the acute stages of infection. Western immunoblot assays were used to assess the development of antibody responses towards the virus.
Rabies Research and Diagnostic Group, Veterinary Laboratories Agency, Addlestone, Surrey. pheaton@hgmp.mrc.ac.uk
P R
PR
Heaton
P
P
Johnstone
J
J
Brownlie
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000302:isolation & purification*; D000917:Antibody Formation; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000821:virology; D002478:Cells, Cultured; D004279:DNA, Viral / Q000302:isolation & purification; D005434:Flow Cytometry; D016087:Immunodeficiency Virus, Bovine / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D016180:Lentivirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D007962:Leukocytes / Q000821:virology; D016133:Polymerase Chain Reaction; D018523:Tropism
D000914:Antibodies, Viral; D004279:DNA, Viral