Improved conditions for the removal of 2-oxoacyl groups from the N-terminus of proteins.
Proteins with R-CO-CO-NH- at the N-terminus, rather than the usual R-CH(-NH3+)-CO-NH-, are produced by non-enzymic transamination and also occur in the pyruvoyl enzymes. The oxoacyl group may be specifically removed from a model peptide, in yields of 70-80%, by treating them in 0.1 M phosphate buffer at 37 degreesC for 24 h with 25 mM of the N-phosphonomethyl derivative of phenylene-1,2-diamine. This provides mild conditions for the stepwise removal of N-terminal residues without denaturation.
Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.
M
M
Sunde
M J
MJ
Sparkes
H B
HB
Dixon
D012437:Adenosylmethionine Decarboxylase / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004590:Electrophoresis, Paper; D009842:Oligopeptides / Q000737:chemistry; D009943:Organophosphorus Compounds; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry*; D011761:Pyrrolidonecarboxylic Acid / Q000031:analogs & derivatives
C115466:N-(2-aminomethyl)aminomethylphosphonic acid; D009842:Oligopeptides; D009943:Organophosphorus Compounds; D011506:Proteins; C115467:pyroglutamyl-alanyl-tyrosine; D012437:Adenosylmethionine Decarboxylase; D011761:Pyrrolidonecarboxylic Acid