Interferon yield and MHC antigen expression of human medulloblastoma cells and its suppression during dibutyryl cyclic AMP-induced differentiation: do medulloblastoma cells derive from bipotent neuronal and glial progenitors?
1. Human medulloblastoma (ONS-76), a central nervous system (CNS)-derived undifferentiated cell line, was found to possess glial characteristics as defined by responses in the interferon (IFN) system; ONS-76 cells produced as much IFN-beta as human fibroblast and glioma cells by viral infection and poly(I):poly(C) induction. 2. Major histocompatibility complex (MHC) class I antigens were also induced under IFN-beta stimulation. ONS-76 cells expressed neurofilament protein, as shown by Northern blot analysis, and morphological differentiation was induced by dibutyryl cyclic AMP (dcAMP). 3. Expression of IFN-beta and MHC class I antigens was suppressed in ONS-76 cells during the dcAMP-induced differentiation. 4. These results showed that ONS-76 cells possessed a glial property in IFN system responses and a neuronal property in cytoskeleton protein, suggesting that the precursors of medulloblastoma may be characterized as bipotent neuronal and glial progenitors in CNS.
Park
K C
KC
Institute for Molecular and Cellular Biology, Osaka University, Japan.
Shimizu
K
K
Hayakawa
T
T
D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage; D048429:Cell Size; D002528:Cerebellar Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002675:Child, Preschool; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D015395:Histocompatibility Antigens Class I / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology; D016899:Interferon-beta / Q000096:biosynthesis* / Q000235:genetics; D008527:Medulloblastoma / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000166:cytology; D009522:Newcastle disease virus / Q000502:physiology; D011070:Poly I-C / Q000494:pharmacology; D013234:Stem Cells / Q000166:cytology*; D014407:Tumor Cells, Cultured / Q000187:drug effects
D015395:Histocompatibility Antigens Class I; D007369:Interferon Inducers; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D003994:Bucladesine; D016899:Interferon-beta; D011070:Poly I-C