Fully automated high-performance liquid chromatography of ciprofloxacin with direct injection of plasma and Mueller-Hinton broth for pharmacokinetic/pharmacodynamic studies.
An isocratic high-performance liquid chromatographic method with column switching and direct injection has been developed to determine ciprofloxacin in plasma and Mueller-Hinton broth. An on-line dilution of the sample was performed with a loading mobile phase consisting of 173 mM phosphoric acid. The analyte was retained on a LiChrocart 4-4 precolumn filled with a LiChrospher 100 RP18, 5 microm. An electric-actuated system with two six-port valves allowed a clean-up step with a mixture 20 mM phosphate buffer (pH 3.5)-methanol (97:3, v/v) and the transfer of the analyte by a back-flush mode to a 150 x 4.6 mm I.D. column packed with a Kromasil C8 5 microm, using a mobile phase of 20 mM phosphate buffer (pH 3.5)-acetonitrile (85:15, v/v). Fluorescence detection allowed a quantification limit of 0.078 microg/ml with a 40-microl sample size. The method was evaluated to determine its usefulness in studying the pharmacokinetic/pharmacodynamic behaviour of ciprofloxacin in an in vitro model.
Pharmacokinetic and Clinical Pharmacy Laboratory, Haut Lévêque Hospital and University Victor Segalen Bordeaux II, Pessac, France.
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Ducint
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Fourtillan
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Saux
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D002939:Ciprofloxacin / Q000097:blood* / Q000493:pharmacokinetics / Q000494:pharmacology; D003470:Culture Media; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D003470:Culture Media; D002939:Ciprofloxacin