Mycobacterium-mediated chemokine expression in pleural mesothelial cells: role of C-C chemokines in tuberculous pleurisy.
Pulmonary tuberculosis is characterized by granulomatous inflammation with an extensive infiltration of mononuclear phagocytes, but the mechanisms of phagocyte recruitment to the pleural space is unknown. In this study, pleural fluid from patients with tuberculosis contained significantly (P<.001) more biologically active MIP-1alpha and MCP-1 (C-C cytokines) than did effusions from patients with congestive heart failure. Antigenic MIP-1alpha and MCP-1 was detected by immunocytochemistry in pleural biopsy sections of patients with tuberculous pleurisy. In vitro, pleural mesothelial cells stimulated with bacille Calmette-Guérin (BCG) or interferon (IFN)-gamma produced MIP-1alpha and MCP-1. Reverse transcription-polymerase chain reaction studies confirmed that both BCG and IFN-gamma induced MIP-1alpha and MCP-1 expression in mesothelial cells, demonstrating that mesothelial cell-derived C-C chemokines play a biologically important role in the recruitment of mononuclear cells to the pleural space.
Department of Pulmonary Medicine, Veterans Affairs Medical Center, Indiannapolis, Indiana, USA.
K A
KA
Mohammed
N
N
Nasreen
M J
MJ
Ward
K K
KK
Mubarak
F
F
Rodriguez-Panadero
V B
VB
Antony
D001706:Biopsy; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D002634:Chemotaxis, Leukocyte; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D006333:Heart Failure / Q000378:metabolism; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D009161:Mycobacterium / Q000378:metabolism*; D010994:Pleura / Q000378:metabolism* / Q000382:microbiology; D010996:Pleural Effusion / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014396:Tuberculosis, Pleural / Q000378:metabolism*
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D019402:Macrophage Inflammatory Proteins; D012333:RNA, Messenger