Juvenile female Litomosoides sigmodontis produce an excretory/secretory antigen (Juv-p120) highly modified with dimethylaminoethanol.
A 120 kDa antigen produced by juvenile female Litomosoides sigmodontis (Juv-p120) was isolated and purified. The amino acid composition of the molecule was determined. Juv-p120 was shown to be highly modified with N,N-dimethyl-aminoethanol (28.4 mol%). Treatment of Juv-p120 with potassium hydroxide (beta-elimination) or with sodium m-periodate leads to the destruction of epitopes recognized by antibodies immune affinity-purified with isolated Juv-p120. Juvenile L. sigmodontis were shown to release Juv-p120 into the pleural cavity of infected Mastomys coucha before the onset of patency.
Biochemisches Institut am Klinikum, Justus-Leibig-Universität, Giessen, Germany.
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M
Hintz
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Schares
A
A
Taubert
R
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Geyer
H
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Zahner
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Stirm
F J
FJ
Conraths
D000581:Amidohydrolases / Q000737:chemistry; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D015153:Blotting, Western; D002241:Carbohydrates / Q000032:analysis; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003642:Deanol / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000737:chemistry; D005260:Female; D005368:Filariasis / Q000276:immunology; D005370:Filarioidea / Q000276:immunology*; D006026:Glycoside Hydrolases / Q000737:chemistry; D006596:Hexosaminidases / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D009115:Muridae; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; D010504:Periodic Acid / Q000737:chemistry; D017680:Potassium Compounds / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D011817:Rabbits; D016205:Sigmodontinae; D010738:Type C Phospholipases / Q000737:chemistry
D000596:Amino Acids; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D002241:Carbohydrates; D006878:Hydroxides; D017680:Potassium Compounds; D010504:Periodic Acid; D003642:Deanol; C009288:metaperiodate; D010738:Type C Phospholipases; D006026:Glycoside Hydrolases; D006596:Hexosaminidases; C060638:glycopeptide alpha-N-acetylgalactosaminidase; D019286:Endopeptidase K; D000581:Amidohydrolases; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; C029943:potassium hydroxide