Increased production of TNF-alpha and decreased levels of dienoic eicosanoids, IL-6 and IL-10 in mice fed menhaden oil and juniper oil diets in response to an intraperitoneal lethal dose of LPS.
Eicosapentaenoic acid (EPA) and the non-methylene interrupted fatty acids (NMIFA) displace arachidonic acid (AA: 20:4omega6 -5,8,11,14) in the membrane phospholipids. Unlike EPA (20:5omega3 -5,8,11,14,17), the NMIFA (20:3omega6 -5,11,14 and 20:4omega3 -5,11,14,17) lacking the delta-8 double bond are not substrates for the formation of eicosanoids. For 20 days, the mice were fed diets containing 5wt% dietary fats from various sources. The magnitudes in the production of eicosanoids and cytokines produced in response to an intraperitoneal injection of endotoxin in mice fed menhaden fish oil (MO) diets enriched with EPA were compared with those maintained on juniper oil (JO) containing NMIFA or on safflower oil (SO), a major source of the AA precursor, linoleic acid. The levels of PGE2, 6-keto-PGF1alpha and TXB2 were markedly lower (P < 0.01) in animals fed either MO or JO diets compared to the controls. The plasma levels of tumor necrosis factor (TNF)-alpha were significantly higher (P < 0.05) with a concomitant decrease of interleukin (IL)-6 and of IL-10 in mice fed MO or JO diets (P < 0.01) compared to those fed SO diet. These data suggest that the effects of consuming NMIFA of JO despite their inability to form eicosanoids are similar to those of feeding EPA which forms biologically active alternate metabolites.
Department of Surgery, Harvard Institutes of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
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Zhong
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D015121:6-Ketoprostaglandin F1 alpha / Q000097:blood; D000818:Animals; D016207:Cytokines / Q000097:blood*; D015232:Dinoprostone / Q000097:blood; D015777:Eicosanoids / Q000097:blood*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D018653:Juniperus; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000633:toxicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D010938:Plant Oils / Q000494:pharmacology*; D012450:Safflower Oil / Q000494:pharmacology; D013929:Thromboxane B2 / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D016207:Cytokines; D015777:Eicosanoids; D005231:Fatty Acids, Unsaturated; D005395:Fish Oils; D015850:Interleukin-6; D008070:Lipopolysaccharides; D010938:Plant Oils; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C033266:Menhaden oil; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; D012450:Safflower Oil; D015232:Dinoprostone; C115013:juniper berry oil