A hydrophobic peptide (VAP-peptide) of the silkworm, Bombyx mori: a unique role for adult activity proposed from gene expression and production at the terminal phase of metamorphosis.
A unique hydrophobic peptide (VAP-peptide) isolated from male adult heads of the silkworm, Bombyx mori, has been shown to act as a synergist to the diapause hormone when administered exogenously. Here, we investigated the true role of the endogenous VAP-peptide on differentiation and development of adult organs in the silkworm. By northern blot analyses, the VAP-peptide gene was shown to be exclusively expressed at the terminal phase of adult development in epithelial tissues, especially in the wing and the thoracic integument. In situ hybridization analysis revealed that the gene was highly expressed in the epidermal cells of the wing vein and the thoracic integument. The stage- and tissue-dependent gene expression were clearly correlated to the accumulation profile of VAP-peptide. In the adult thoracic integument, VAP-peptide was predominantly deposited in the cuticle layer. Affinity chromatography indicated the ability of VAP-peptide to bind to chitin. Based on its expression patterns, localization, and chemical properties, VAP-peptide is conceived to be a structural protein that participates in mechanical strengthening of specific cuticle structures, supporting their physical requirements in the adult life of the silkworm.
Shiomi
K
K
Graduate School of Bioagricultural Sciences, Nagoya University, Japan.
Niimi
T
T
Sato
Y
Y
Imai
K
K
Yamashita
O
O
D000818:Animals; D012831:Bombyx / Q000254:growth & development* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D006257:Head; D017403:In Situ Hybridization; D007301:Insect Hormones / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008297:Male; D008675:Metamorphosis, Biological / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D013909:Thorax; D014158:Transcription, Genetic
D007301:Insect Hormones; C115047:VAP peptide