[Fasciola hepatica antigens. Use in the diagnosis of human fascioliasis].
The ELISA technique was standardized for detecting antibodies against Fasciola in 25 serum samples from patients-infected with Fasciola hepatica using excretion-secretion antigens (ESA), tegument antigen and somatic antigen. A study was made of serum samples from 46 patients with other parasites such as Ascaris lumbricoides, Trichuris trichiura, Entamoeba histolytica, Schistosoma mansoni and Giardia lamblia. The cohort value for each trial was established after studying 100 serum samples from healthy people as negative controls taking as criterion the mean optical density plus 2 standard deviations from the mean. Regarding this group and using ESA, we obtained specificity and sensitivity.
Instituto de Medicina Tropical Pedro Kouri.
A M
AM
Espino Hernández
N
N
Seuret
C
C
Morlote
B
B
Duménigo Ripoll
D000818:Animals; D000909:Antibodies, Helminth / Q000032:analysis*; D000947:Antigens, Helminth; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis*; D006801:Humans; D012680:Sensitivity and Specificity
D000909:Antibodies, Helminth; D000947:Antigens, Helminth
Antígenos de Fasciola hepatica. Su utilidad en el diagnóstico de la fasciolasis humana.