A high resolution HLA class I and class II matching method for bone marrow donor selection.
HLA matching in bone marrow transplantation has an important role in determining successful outcome. However HLA typing of both potential related and unrelated donors can be both time-consuming and laborious, and does not always resolve accurately the true level of histocompatibility. We have utilised a method, reference strand mediated conformation analysis (RSCA), which is technically simple and allows high resolution matching for all HLA loci, for the typing of 48 patients and their potential 120 donors. The results indicate that RSCA can detect many mismatches that are not routinely identified by conventional HLA typing methods. In addition, RSCA can be applied for the simultaneous analysis of multiple potential BM donor samples in order to quickly identify the best match for each patient.
Anthony Nolan Research Institute, The Royal Free Hospital School of Medicine, London, UK.
J R
JR
Argüello
A M
AM
Little
E
E
Bohan
D
D
Gallardo
J
J
O'Shea
I A
IA
Dodi
J M
JM
Goldman
J A
JA
Madrigal
D000483:Alleles; D016026:Bone Marrow Transplantation / Q000276:immunology*; D004247:DNA / Q000097:blood / Q000737:chemistry / Q000235:genetics; D005069:Evaluation Studies as Topic; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D015395:Histocompatibility Antigens Class I / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000235:genetics; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D014019:Tissue Donors
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D004247:DNA