Two iron-regulated putative ferric siderophore receptor genes in Bordetella bronchiseptica and Bordetella pertussis.
Two iron-regulated genes with deduced homology to TonB-dependent ferric siderophore receptors were cloned from Bordetella bronchiseptica by screening a library of TnphoA insertion mutants. bfrB and bfrC were iron-repressed in B. bronchiseptica by a Fur-dependent mechanism, and were expressed from promoters overlapped by potential Fur-binding sites. Both genes were highly conserved among Bordetella species and were also iron-regulated in Bordetella pertussis. bfrB and bfrC mutants of both species and a bfrB-bfrC double mutant of B. bronchiseptica had no discernible defects in utilization of known iron sources for Bordetella.
Beall
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Centers for Disease Control and Prevention, Respiratory Diseases Laboratory Section, Atlanta, GA 30333, USA.
D000469:Alkaline Phosphatase / Q000032:analysis; D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D016950:Bordetella bronchiseptica / Q000235:genetics* / Q000378:metabolism; D001886:Bordetella pertussis / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004758:Enterobactin / Q000494:pharmacology; D005290:Ferric Compounds / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D006877:Hydroxamic Acids; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010957:Plasmids / Q000737:chemistry; D012313:RNA / Q000737:chemistry; D012329:RNA, Bacterial / Q000737:chemistry; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D017262:Siderophores / Q000378:metabolism* / Q000494:pharmacology
D001425:Bacterial Outer Membrane Proteins; C115331:BfrB protein, Bordetella; C115332:BfrC protein, Bordetella; D004269:DNA, Bacterial; D005290:Ferric Compounds; D006877:Hydroxamic Acids; C034477:RNA primers; D012329:RNA, Bacterial; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; D017262:Siderophores; C092056:alcaligin; C035902:siderophore receptors; D004758:Enterobactin; D012313:RNA; D000469:Alkaline Phosphatase