Use of scavenger beads to remove excess labeling reagents from capillary zone electrophoresis samples.
In many cases, samples for capillary zone electrophoresis (CZE) are derivatized with a chromophore or fluorophore to enhance their detectability. To ensure efficient labeling, a large excess of labeling agent is often used, which leads to the presence of a large peak for unreacted reagent. Here we report that excess reagent can be reacted with "scavenger beads" carrying an appropriate functional group to remove it from the sample solution. We present examples of removal of aminonaphthalene mono-, di-, and trisulfonic acid from mixtures in which they were used to label mono- or oligosaccharides by reductive amination. Aldehyde-containing scavenger beads were made by oxidizing Sephadex G-50 beads with sodium periodate. These were added to the labeling reaction mixtures after the reductive amination of the sugars was complete. Almost complete elimination of the peak from the labeling agent could be achieved.
Mort
A J
AJ
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater 74078-3035, USA. amort@biochem.okstate.edu
Zhan
D
D
Rodriguez
V
V
D000447:Aldehydes; D003911:Dextrans; D019075:Electrophoresis, Capillary / Q000379:methods*; D007202:Indicators and Reagents; D008863:Microspheres; D009281:Naphthalenes; D009844:Oligosaccharides; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000737:chemistry; D013451:Sulfonic Acids
D000447:Aldehydes; D003911:Dextrans; D007202:Indicators and Reagents; D009281:Naphthalenes; D009844:Oligosaccharides; D013451:Sulfonic Acids; D010504:Periodic Acid; C025614:sephadex; C009288:metaperiodate