Growth inhibition of pancreatic tumor cells by modified antisense oligodeoxynucleotides.
BACKGROUND
Pancreatic adenocarcinomas are largely resistant to apoptosis. More than 50% of pancreatic tumors reveal mutations in the p53 tumor suppressor gene.


METHODS
We investigated the growth of pancreatic tumor cells after downregulation of p53 protein expression by antisense oligodeoxynucleotides.


RESULTS
Proliferation and p53 expression of PancTu-I cells overexpressing mutant p53 protein were inhibited by antisense oligodeoxynucleotide treatment. When analyzed, two of three other pancreatic tumor cell lines with mutated p53 were also inhibited in their growth. Two of two wild-type (wt) p53 pancreatic tumor cells were not significantly influenced by p53 expression and were, only to a lesser extent, affected in their proliferation. K562 cells (lacking p53 mRNA) and normal human skin fibroblasts used as a target mismatch control showed no changes in proliferation rates with treatment. The different biological effects in the various cells were not caused by differences in the uptake of the oligodeoxynucleotides as monitored by confocal laser-scanning microscopy.


CONCLUSIONS
Truncation and 5'- and 3'-lipophilic modifications of the oligodeoxynucleotides drastically enhanced the growth inhibition of PancTu-I cells, which were resistant to apoptosis-inducing agents. Furthermore, a higher sequence-specificity of the observed effects was achieved with these compounds.
Fiedler
A
A
Research Unit for Molecular Oncology, Clinic for General and Thoracic Surgery, Christian-Albrechts-University, Kiel, Germany.
Knorre
C
C
Franke
Y
Y
Henne-Bruns
D
D
Kremer
B
B
Lüttges
J
J
Maier
M
M
Gerster
M
M
Bleicher
K
K
Bayer
E
E
Kalthoff
H
H
D000230:Adenocarcinoma / Q000473:pathology*; D002452:Cell Count; D002460:Cell Line; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D020014:K562 Cells / Q000187:drug effects; D020319:Oligodeoxyribonucleotides, Antisense / Q000494:pharmacology*; D010190:Pancreatic Neoplasms / Q000473:pathology*; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000032:analysis
D020319:Oligodeoxyribonucleotides, Antisense; D016159:Tumor Suppressor Protein p53