Placental iodothyronine deiodinase III and II ratios, mRNA expression compared to enzyme activity.
Iodothyronine deiodinases III and II (D3 and D2) specific enzyme activities in human placenta both decrease with gestational age. The relation of the enzyme activities with their respective mRNA expression was investigated by semi-quantitative RT-PCR on human placenta mRNA. To investigate if RT-PCR is a useful tool to detect iodothyronine deiodinase mRNA, several tissues were screened using this technique. In all tissues with iodothyronine deiodinase enzyme activity, the corresponding RT-PCR product is present. Similar to D3 specific enzyme activity, the amount of D3 mRNA in placenta declines with gestational age. The ratios of the D3/D2 enzyme activity and mRNA expression in placenta do not correlate. D3 enzyme activity shows an average 300-fold excess compared to D2 activity. However, semi-quantitative PCR analysis of D3 and D2 mRNA shows a D3/D2 ratio varying from 0.05 to 52. These results suggest that the placental D2 mRNA amplified is not translated into placental D2 enzyme activity.
Stulp
M R
MR
Academic Medical Center, Laboratory of Pediatric and Neonatal Endocrinology, University of Amsterdam, The Netherlands. m.r.stulp@amc.uva.nl
de Vijlder
J J
JJ
Ris-Stalpers
C
C
D005260:Female; D005333:Fetus / Q000201:enzymology; D015870:Gene Expression; D005865:Gestational Age; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics / Q000378:metabolism*; D009928:Organ Specificity; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000097564:Iodothyronine Deiodinase Type II
D007453:Iodide Peroxidase; D012333:RNA, Messenger; D000097564:Iodothyronine Deiodinase Type II