[Normalization of an ultramicroELISA for the detection of antibodies against human Parvovirus B19].
An ultramicroELISA (UME) method was normalized for the detection of antibodies to serum human parvovirus B19. The optimum antigen concentration determined was 400 ng/ml, serum dilution was of 1:100; and the conjugate work dilution was of 1:2,000. 11 paired serum samples were also evaluated and antibodies were detected. The usefulness of the analyzed system is discussed.
Instituto de Medicina Tropical Pedro Kouri.
L
L
Morier Díaz
A
A
Castillo Alvarez
J
J
Laferté Serrano
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards*; D006801:Humans; D016732:Parvovirus B19, Human / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral
Normalización de un ultramicroELISA para la detección de anticuerpos a Parvovirus humano B19.