[Characterization of phenolic glycolipid-I (PGL-I) like antigen in renal cell carcinoma].
BACKGROUND
We investigated whether phenolic glycolipid-I (PGL-I) produced by Mycobacterium leprae (M. leprae), can be used as a marker of renal tumors.


METHODS
Using a preparted anti-PGL-I monoclonal antibody (SF-I), anti-PGL-I group antibodies and PGL-I group antigens were detected and PGL-I immunohistologic staining were performed.


RESULTS
The titer of anti-PGL-I antibody in patients with renal cell carcinoma was 0.283 +/- 0.103, showing a trend of rising titer of anti-PGL-I group antibody with higher cytologic atypia. When compared by the extent of tumor infiltration, a high antibody titer was observed in stage 4 infiltration group. Regarding the PGL-I group antigen that was detected using SF-1 in the urine of patients with renal cell carcinoma, no relationships between the positive rte of the antigens with common antigenicity to PGL-I and the grade of carcinoma were observed. However, in terms of the extent of tumor infiltration, a trend that the positive rate for PGL-I group antigen increased with the progression of cancer stage was seen. The Western blot assay of the urine of patients with renal cell carcinoma following electrophoresis revealed a change around 40 KD. The immunohistologic stains with SF-1 disclosed a predominance in the proximal kidney tubule.


CONCLUSIONS
Using PGL-I group antigen and PGL-I group antibody as markers of renal cell carcinoma, a rapid and precise measurement may be provided.
Department of Urology, Kinki University School of Medicine.
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H
Matsuda
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D014408:Biomarkers, Tumor / Q000032:analysis*; D002292:Carcinoma, Renal Cell / Q000175:diagnosis*; D005260:Female; D006017:Glycolipids / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D014408:Biomarkers, Tumor; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae