Quaternary structure of UEA-II, the chitobiose specific lectin from gorse.
The chitobiose specific Ulex europaeus lectin II crystallizes in space group P3221 with unit-cell dimensions a = b = 105.54, c = 176.26 A. The asymmetric unit contains a complete lectin tetramer. The crystals were shown to diffract to 4.5 A on a rotating-anode source and to 2.7 A at the Daresbury synchrotron source. Molecular replacement and subsequent rigid-body refinement using data to 4.5 A yielded a solution corresponding to a tetramer very similar to that of phytohemagglutinin-L and soybean agglutinin. The monomers in the Ulex lectin tetramer are rotated approximately 5 degrees compared with the phytohemagglutinin-L and soybean agglutinin structures.
Laboratorium voor Ultrastruktuur, Vlaams Interinuversitair Instituut voor Biotechnologie, Vrije Universiteit Brussel, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium.
M H
MH
Dao-Thi
P
P
Rizkallah
L
L
Wyns
F
F
Poortmans
R
R
Loris
D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D003460:Crystallization; D018360:Crystallography, X-Ray; D007887:Fabaceae / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010946:Plants, Medicinal; D011487:Protein Conformation; D013379:Substrate Specificity
D037102:Lectins; D037121:Plant Lectins; C036850:Ulex europaeus lectins