Colorimetric assay of sialic acid by a methyl-3-benzothiazolinone-2-hydrazone reactant.
A colorimetric assay of sialic acid has been developed in which bound sialic acid is oxidized by periodic acid so as to quantitatively release formaldehyde. In the second step of the reaction formaldehyde reacts with methyl-3-benzothiazolinone-2-hydrazone, giving a colored compound. This method does not require a prior hydrolysis and presents the advantage of great accuracy.
Massamiri
Y
Y
Departement de Biochimie, U. E. R. des Sciences Pharmaceutiques et Biologiques, Chatenay Malabry, France.
Beljean
M
M
Durand
G
G
Feger
J
J
Pays
M
M
Agneray
J
J
D052160:Benzothiazoles; D003124:Colorimetry / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006835:Hydrazones; D007202:Indicators and Reagents; D019158:N-Acetylneuraminic Acid / Q000032:analysis*; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012015:Reference Standards; D013844:Thiazoles
D052160:Benzothiazoles; D006835:Hydrazones; D007202:Indicators and Reagents; D013844:Thiazoles; D010504:Periodic Acid; C009288:metaperiodate; D019158:N-Acetylneuraminic Acid; C002536:3-methyl-2-benzothiazolone hydrazone