[Cloning and analysis of DNA from interband regions 85D9/D10 and 86B4/B6 of Drosophila melanogaster polytene chromosomes].
A recently developed complex approach based on electron microscopic mapping of the P element inserts in the interband regions of Drosophila polytene chromosomes was used for the cloning and sequencing of DNA from the 85D9/D10 and 86B4/B6 interband regions. The in situ hybridization technique showed that these DNA fragments belonged to the fraction of unique sequences of the Drosophila genome. Comparison of the cloned sequences with the earlier cloned 61C7/C8 interband region was carried out. Our findings indicated that these clones were represented by AT-rich sequences (over 80% AT) interspersed with segments with a random nucleotide composition. The interband regions were shown to contain large amounts of polypurine, polypyrimidine and purine-pyrimidine stretches. This structural organization is typical of DNA sequences that are able to form noncanonical secondary structures and is similar to that of matrix-attached DNA regions. Cloned interband DNA regions were shown to contain short open reading frames with an unknown functional activity.
Schwartz
Iu V
IuV
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia. schwartz@bionet.nsc.ru
Demakov
S A
SA
Khimulev
I F
IF
D000818:Animals; D001482:Base Composition; D002871:Chromosome Banding; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D017403:In Situ Hybridization; D008854:Microscopy, Electron
D004247:DNA
Klonirovanie i analiz DNK iz mezhdiskovykh raĭonov 85D9/D10 i 86B4/B6 politennykh kromosom Drosophila melanogaster.