3-Bromoacetylamino benzoylurea (3-BAABU), a new antimicrotubule cancericidal agent applied in cytogenetic analysis in hematology.
3-Bromoacetylamino benzoylurea (3-BAABU) is a newly synthesized antimicrotubule cancericidal compound. In the present study, we investigated the possibility of using 3-BAABU as a mitotic blocking agent for hematologic karyotyping. Treatment with 3-BAABU caused scattering of metaphase chromosomes throughout the cytoplasm both in phytohemagglutinine (PHA)-stimulated human lymphocytes and in human leukemic cells. Kinetic showed a rapid uptake of 3-BAABU by treated cells and irreversibility of its effect. Using 3-BAABU in routine procedure, a karyotype of lymphocytes from a normal male was 46, XY, with normal structure and CEM leukemic line was 85, XX, in a representative spread with abnormalities similar to reports using other blocking agents. Using 3-BAABU in spectral karyotyping, details of translocations in CEM leukemic cells were readily detected in several chromosomes, such as 7 [t(7;11)], 8 [t(8;9)], 9 [t(8;9) & t(9;19)], 11 [t(7;11)], 16 [t(16;18;20)] and 20 [t(1;20)]. 3-BAABU displayed two important characters in cytogenetics, 1) it caused dispersion of chromosomes, avoiding chance of overlap; 2) compared to the conventional mitotic blocking agent, vinblastine sulfate, 3-BAABU exhibited much gentle effect on chromosomes, thus providing more flexibility in time to perform karyotyping.
Division of Neoplastic Diseases of Department of Medicine, Mount Sinai School of Medicine, New York, NY 10029, USA.
J D
JD
Jiang
Y
Y
Wang
C A
CA
Janish
J F
JF
Holland
J G
JG
Bekesi
D000970:Antineoplastic Agents / Q000493:pharmacokinetics / Q000494:pharmacology*; D002877:Chromosomes, Human / Q000187:drug effects / Q000648:ultrastructure*; D003582:Cytogenetics / Q000379:methods; D053938:DNA Fragmentation; D006801:Humans; D007621:Karyotyping; D007700:Kinetics; D015458:Leukemia, T-Cell; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D008297:Male; D008870:Microtubules / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D014178:Translocation, Genetic; D014407:Tumor Cells, Cultured; D014508:Urea / Q000493:pharmacokinetics / Q000494:pharmacology*
C112347:3-bromoacetylamino benzoylurea; D000970:Antineoplastic Agents; D014508:Urea