The enzymic synthesis of beta-[32P]UDP-N-acetylglucosamine.
Cells of Micrococcus sp. 2102 incorporate inorganic [32P]phosphate from the medium into the sugar-phosphate polymer of the wall. Controlled acid hydrolysis of sodium dodecyl sulphate-extracted cells gives N-acetylglucosamine 6-[32P]-phosphate which can be purified by ion-exchange chromatography and incubated with UTP in the presence of crude preparations of phosphoacetylglucosamine mutase from Neurospora crassa and UTP:N-acetylglucosamine 1-phosphate phosphotransferase from Bacillus licheniformis which act in concert to synthesise beta-[32P]UDP-N-acetylglucosamine.
Microbiological Chemistry Research Laboratory, University of Newcastle upon Tyne, United Kingdom.
J
J
Heptinstall
P J
PJ
Ward
I C
IC
Hancock
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000302:isolation & purification; D001407:Bacillus / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D008837:Micrococcus / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D009492:Neurospora crassa / Q000201:enzymology; D010761:Phosphorus Radioisotopes; D017875:Phosphotransferases (Phosphomutases) / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000096:biosynthesis* / Q000302:isolation & purification
D010761:Phosphorus Radioisotopes; C010004:N-acetylglucosamine 6-phosphate; D014537:Uridine Diphosphate N-Acetylglucosamine; D017351:N-Acetylglucosaminyltransferases; D017875:Phosphotransferases (Phosphomutases); C018718:phosphoacetylglucosamine mutase; D000117:Acetylglucosamine