Removal of sodium dodecyl sulfate from proteins.
Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of protein.
Biochemistry Department, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
O H
OH
Kapp
S N
SN
Vinogradov
D000818:Animals; D001665:Binding Sites; D002021:Buffers; D002417:Cattle; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification*; D012967:Sodium Dodecyl Sulfate / Q000302:isolation & purification*; D014867:Water
D002021:Buffers; D011506:Proteins; D014867:Water; D012967:Sodium Dodecyl Sulfate