Immunolocalization of CD44 in the dystrophic rat retina.
The distribution of the cell surface adhesion/receptor molecule CD44 was studied in retinas of the Royal College of Surgeons (RCS) rat which exhibits an inherited retinal dystrophy. In this animal model, the retinal pigment epithelium fails to phagocytize shed photoreceptor outer segment material, a membranous debris layer accumulates in the subretinal space and the photoreceptor cells degenerate. Using immunoperoxidase and immunogold labeling, CD44 was localized to Müller cell apical microvilli in normal rat retinas, as noted in other species. For the RCS rat, immunoperoxidase labeling of 18 day and 1 month retinas showed the typical microvillar labeling pattern. At 2 months postnatal, following degeneration of most of the photoreceptors, a more condensed band of microvillar label was observed. At 3 months, when photoreceptor degeneration was virtually complete, only distinct regions of dense label remained between the neural retina and debris zone. Upon ultrastructural and immunogold analysis, these regions were found to contain closely packed Müller cell microvilli. At all ages studied, labeling for CD44 in the inner retina did not increase, as it does in other forms of retinal degeneration which lack a debris zone. However, by 3 months the debris zone was labeled for CD44 indicating that CD44 molecules remain on Müller cell microvilli and processes which have extended into and become part of the debris zone. This may be caused by an altered distribution of still undetermined ligands for CD44 which are present within the interphotoreceptor matrix of the RCS rat retina.
Chaitin
M H
MH
Department of Anatomy and Cell Biology and North Texas Eye Research Institute, University of North Texas Health Science Center at Fort Worth, Fort Worth, Texas, USA.
Brun-Zinkernagel
A M
AM
D000818:Animals; D020296:Animals, Congenic; D018960:Hyaluronan Receptors / Q000032:analysis*; D007124:Immunoenzyme Techniques; D016253:Microscopy, Immunoelectron; D009137:Muscular Dystrophy, Animal / Q000378:metabolism*; D051381:Rats; D011916:Rats, Inbred F344; D011922:Rats, Mutant Strains; D012160:Retina / Q000737:chemistry*
D018960:Hyaluronan Receptors