Pattern of apolipoprotein D immunoreactivity in human brain.
Presence of intracytoplasmatic apolipoprotein D (apo D), a lipophilic ligand transporter, was investigated in normal human brains between 20 and 55 years, using an anti-human apolipoprotein D antibody and extravidin-biotin-enhanced immunohistochemistry. Apo D immunoreactivity was found in neuroglial cells of white matter in all sampled brain regions studied but also in pial cells and perivascular cells. Immunoreactive neurons do not present a uniform pattern throughout the gray matter. The pons and the brainstem show a high immunoreactivity for apo D in several nuclei (olivary, arciforme, cuneado, raphe). In the cerebellum the immunoreactivity appears in some neurons of the Purkinje layer. Finally in the cerebral cortex apo D positive neurons were not observed. These results suggest that apo D role may vary depending of cellular synthesis or location.
Departamento de Morfología y Biología Celular, Facultad de Biología y Medicina, Universidad de Oviedo, Spain.
A
A
Navarro
J
J
Tolivia
A
A
Astudillo
E
E
del Valle
D000328:Adult; D001053:Apolipoproteins / Q000378:metabolism*; D053399:Apolipoproteins D; D015415:Biomarkers; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002529:Cerebellar Nuclei / Q000737:chemistry / Q000166:cytology; D002540:Cerebral Cortex / Q000737:chemistry / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry / Q000166:cytology; D010296:Parietal Lobe / Q000737:chemistry / Q000166:cytology; D011689:Purkinje Cells / Q000737:chemistry / Q000166:cytology
D001053:Apolipoproteins; D053399:Apolipoproteins D; D015415:Biomarkers