Two modes of survival of fission yeast without telomerase.
Deletion of the telomerase catalytic subunit gene trt1+ in Schizosaccharomyces pombe results in death for the majority of cells, but a subpopulation survives. Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller number maintain their telomeres presumably through recombination. When the telomeric DNA-binding gene taz1+ is also deleted, trt1- taz1- survivors use the recombinational mode more frequently. Moreover, the massive elongation of telomeres in taz1- cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast.
Nakamura
T M
TM
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA.
Cooper
J P
JP
Cech
T R
TR
D015825:Chromosomes, Fungal / Q000235:genetics / Q000378:metabolism*; D015342:DNA Probes; D004271:DNA, Fungal / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D012313:RNA; D011995:Recombination, Genetic; D012568:Schizosaccharomyces / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D016615:Telomere / Q000235:genetics* / Q000378:metabolism*; D034501:Telomere-Binding Proteins
D015342:DNA Probes; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D011506:Proteins; D029702:Schizosaccharomyces pombe Proteins; D034501:Telomere-Binding Proteins; C104344:taz1 protein, S pombe; C422952:telomerase RNA; C509188:trt1 protein, S pombe; D012313:RNA; D019098:Telomerase