Pulse exposure of cultured rat neurons to aluminum-maltol affected the axonal transport system.
Although chronic aluminum neurotoxicity has been well established, the mechanism of the toxicity has not been elucidated yet. In order to simplify the study of the aluminum neurotoxicity, we employed the pulse exposure of cultured rat cortical neurons to 250 microM aluminum-maltol for 1 h at the early stage (6 h after plating), which resulted in abnormal distribution of neurofilament L (NFL) and fast axonal transported proteins, whereas the axonal transport of tubulin, actin, and clathrin were not impaired. Otherwise, the pulse exposure of neurons at the late stage (4 days after plating) to the same concentration of aluminum-maltol did not affect the cell morphology and the distribution of NFL. The pulse exposure of cultured neurons to aluminum-maltol at the early stage might affect the axonal transport system of NFL and fast axonal transported proteins.
Department of Neuropsychiatry, Osaka University Medical School, Suita-shi, Japan. kasiwagi@psy.med.osaka-u.ac.jp
Y
Y
Kashiwagi
Y
Y
Nakamura
Y
Y
Miyamae
R
R
Hashimoto
M
M
Takeda
D000199:Actins / Q000032:analysis; D000535:Aluminum / Q000633:toxicity; D000818:Animals; D001370:Axonal Transport / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D002966:Clathrin / Q000032:analysis; D016900:Neurofilament Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*; D009498:Neurotoxins / Q000633:toxicity; D009942:Organometallic Compounds / Q000633:toxicity*; D011673:Pulsatile Flow; D011753:Pyrones / Q000633:toxicity*; D051381:Rats; D014404:Tubulin / Q000032:analysis
D000199:Actins; D002966:Clathrin; D016900:Neurofilament Proteins; D009498:Neurotoxins; D009942:Organometallic Compounds; D011753:Pyrones; D014404:Tubulin; C044882:neurofilament protein L; C067527:aluminum maltolate; D000535:Aluminum