Identification and characterization of the glycoprotein E and I genes of canine herpesvirus.
We have determined the sequence of the gE and gI genes of canine herpesvirus (CHV), DFD-6 strain. The gE ORF codes for a 522 a.a. polypeptide with a signal sequence at the amino-terminus and a trans-membrane domain at the carboxy-terminus. The gI ORF codes for a 259 a.a. polypeptide with a signal sequence but no trans-membrane domain. Comparison with another line of CHV indicated that the DFD-6 gI gene underwent a frame-shift mutation which caused the loss of the trans-membrane domain. Antibodies against the gE and gI polypeptides detected a 94 kDa gE and a broad band of gI (55-62 kDa) in DFD-6 infected cells, respectively. The precursor of DFD-6 gE is modified to the mature form by N-linked glycosylation only in the presence of gI. Together with the fact that the gI- mutant of DFD-6 produced smaller plaques, it is suggested that the truncated DFD-6 gI is functional. The precursor of DFD-6 gI is modified to the mature form by N-linked glycosylation only in the presence of gE.
Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Science, The University of Tokyo, Japan.
Y
Y
Nishikawa
X
X
Xuan
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H
Otsuka
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D004285:Dogs; D005260:Female; D005814:Genes, Viral; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics*; D002184:Herpesvirus 1, Canid / Q000235:genetics*; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011817:Rabbits; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014616:Vaccinia virus / Q000235:genetics; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics*
D004279:DNA, Viral; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C116238:glycoprotein E, Canid herpesvirus 1; C116239:glycoprotein I, Canid herpesvirus 1