[Development and evaluation of an ELISA for the detection of IgM antibodies against the hepatitis A virus].
We present the results of the normalization of an IgM capture ELISA method for the diagnosis of type A viral hepatitis with reagents produced in the laboratory and its comparison with the "Diag-A-Hep" commercial ELISA. The results attained were: sensibility by 91%; specificity by 100%; and coincidence of the two systems by 97%. Results are discussed and their relationship with clinical symptoms and epidemiological characteristics is established. The results attained in 13 serum samples taken from patients seen during 2 acute viral hepatitis outbreaks agreed with clinical findings.
Instituto de Medicina Tropical Pedro Kouri.
L
L
Rodríguez Lay
P
P
Más Lago
A
A
Balmaseda
M M
MM
Comellas
G
G
Delgado
R
R
Palomera
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006506:Hepatitis A / Q000175:diagnosis*; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000097:blood*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D011933:Reagent Kits, Diagnostic
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M; D011933:Reagent Kits, Diagnostic
Desarrollo y evaluación de un ELISA para la detección de anticuerpos IgM contra el virus de la hepatitis A.