[Effect of some DNA-intercalators and antioxidants on the phagosome- lysosome fusion and F-actin content in murine peritoneal macrophages].
Effect of DNA-intercalators ethidium bromide (EB, 0.005 and 0.015 mM) and dimidium bromide (DB, 0.005 and 0.010 mM) and antioxidative compounds acetylsalicylic acid (ASA, 0.05 and 0.50 mM) and beta-carotene (0.01, 0.02, 0.05 mM) on the phagosome-lysosome (P-L) fusion and F-actin content in murine peritoneal macrophages were studied. EB, DB, ASA and beta-carotene were found to stimulate P-L fusion and the effect depending on the concentration of compounds tested. The strongest influence as evoked by 0.5 mM of ASA and 0.05 mM of beta-carotene. The compounds tested enhanced the F-actin content in macrophages, especially by the action of beta-carotene (0.05 mM). The obtained data indicate a correlation between P-L fusion stimulation and F-actin content under the influence of compounds tested in murine peritoneal macropheages.
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
T P
TP
Mozhenok
T N
TN
Beliaeva
A G
AG
Bulychev
E A
EA
Leont'eva
D000199:Actins / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001241:Aspirin / Q000494:pharmacology; D002478:Cells, Cultured; D004996:Ethidium / Q000494:pharmacology*; D008247:Lysosomes / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D010588:Phagosomes / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D010617:Phenanthridines / Q000494:pharmacology*; D019207:beta Carotene / Q000494:pharmacology
D000199:Actins; D000975:Antioxidants; D010617:Phenanthridines; D019207:beta Carotene; C035275:dimidium; D004996:Ethidium; D001241:Aspirin
Vliianie nekotorykh DNK-interkaliatorov a antioksidantov na sliianie fagosom s lizosomami i na soderzhanie F-aktina v peritoneal'nykh makrofagakh myshi.