[Use of a dengue anti-complex monoclonal antibody in viral purification].
The purification of different serotypes of dengue virus from a monoclonal antibody which recognizes an epitope present in the four serotypes bond to a Sepharose 4B matrix activated with cyanogen bromide, is reported. Results evidence that the method employed makes quicker the process of purification with a high degree of purity.
Instituto de Medicina Tropical Pedro Kouri.
C
C
Hermida Díaz
M
M
Pupo
M G
MG
Guzmán Tirado
M
M
González Garriga
R
R
Marcet Sánchez
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003716:Dengue Virus / Q000276:immunology* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral
Empleo de un anticuerpo monoclonal anticomplejo dengue en la purificación viral.