Lysis of Paracoccidioides brasiliensis by Zygosporium geminatum.
Zygosporium geminatum, isolated as a contaminant in a culture of the mycelial phase of Paracoccidioides brasiliensis, was lethal to the latter organism. Its lytic action was due to exocellular alpha-1,3- and beta-1,3-glucanases which degraded the P. brasiliensis cell wall. The alpha-1,3-glucanase was more active at 30 degrees C and the beta-1,3-glucanase at 23 degrees C, each having pH 6.0 as its optimum.
Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Microbiología y Biología Celular, Apartado 21827, Caracas 1020A, Venezuela. gsanblas@pasteur.ivic.ve
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San-Blas
D002473:Cell Wall; D043326:Glucan 1,3-beta-Glucosidase; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology* / Q000254:growth & development; D010228:Paracoccidioides / Q000254:growth & development; D013816:Thermodynamics; D001617:beta-Glucosidase / Q000378:metabolism*
D006026:Glycoside Hydrolases; D001617:beta-Glucosidase; D043326:Glucan 1,3-beta-Glucosidase; C021780:exo-1,3-alpha-glucanase