Reverse transcription-polymerase chain reaction and immunohistochemical study of the expression of fibronectin mRNA in human submandibular salivary gland.
The alternative expression of fibronectin mRNA in submandibular salivary glands was analysed using three groups of polymerase chain reaction probes to amplify the cell-binding domain (CBD), and the extra domain (ED)-A and ED-B exon regions in five normal human adults. The fibronectin CBD region was fairly well expressed in the salivary gland while less than trace amounts of ED-A and ED-B exons were expressed. Immunohistochemical staining with an antihuman plasma fibronectin monoclonal antibody, which recognized specifically the CBD region, demonstrated definite positive cytoplasmic staining in the duct-cell area. On the other hand, an anticellular fibronectin ED-A-specific monoclonal antibody was very low positive in duct cells. The results suggest that salivary fibronectin is synthesized in the submandibular salivary gland only, and does not include the ED-A segment. Furthermore, duct cells also produced fibronectin without ED-A.
Doi
S
S
Department of Oral Pathology, Asahi University School of Dentistry, Motosu, Gifu, Japan.
Kawarada
M
M
Hirano
H
H
Takeuchi
H
H
D000328:Adult; D005091:Exons; D005260:Female; D005353:Fibronectins / Q000096:biosynthesis* / Q000235:genetics*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D011487:Protein Conformation; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018987:Salivary Ducts / Q000378:metabolism; D013363:Submandibular Gland / Q000378:metabolism*
D005353:Fibronectins; D012333:RNA, Messenger