Absent myocardial iodine-123-BMIPP uptake and platelet/monocyte CD36 deficiency.
Global absence of myocardial 123I-15-(p-iodophenyl)-3-(R,S)-methyl pentadecanoic acid (BMIPP) uptake is occasionally noted, and it reflects myocardial long-chain fatty acid uptake abnormality. CD36, a membrane glycoprotein expressed on platelet, monocyte and endothelial cells, may contribute to myocardial fatty acid transport, and its deficiency has been reported in a small subset of the population. We hypothesized that CD36 deficiency may be related to absent myocardial BMIPP uptake. Thus, we investigated CD36 expression of platelet/monocyte in patients with absent myocardial BMIPP uptake.


METHODS
Peripheral blood of 7 patients with global absence of myocardial BMIPP uptake (3 of 7 patients in one family) and 3 control subjects were examined in flow cytometric analysis. Platelet/monocyte surface CD36 was detected by using OKM5, an anti-CD36 mouse monoclonal antibody.


RESULTS
There were no apparent relationships between specific clinical symptoms and absent myocardial BMIPP uptake. None of the blood samples of the 7 patients were stained with OKM5 on the platelet/monocyte cell surface, indicating that all of these patients were Type I CD36-deficient subjects. In contrast, all the control subjects showed normal staining.


CONCLUSIONS
The fact that rare Type I CD36 deficiency was observed in all patients with absent myocardial BMIPP uptake suggests that CD36 plays a role in the myocardial long-chain fatty acid uptake process in humans.
Department of Nuclear Medicine, Kanazawa University School of Medicine, Japan.
E H
EH
Hwang
J
J
Taki
S
S
Yasue
M
M
Fujimoto
M
M
Taniguchi
I
I
Matsunari
K
K
Nakajima
S
S
Shiobara
T
T
Ikeda
N
N
Tonami
D000328:Adult; D000368:Aged; D001792:Blood Platelets / Q000276:immunology; D018955:CD36 Antigens / Q000502:physiology; D005227:Fatty Acids / Q000493:pharmacokinetics; D005260:Female; D006321:Heart / Q000000981:diagnostic imaging*; D006801:Humans; D007457:Iodine Radioisotopes; D007462:Iodobenzenes / Q000493:pharmacokinetics; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D009206:Myocardium / Q000378:metabolism*; D010980:Platelet Membrane Glycoproteins / Q000172:deficiency*; D015899:Tomography, Emission-Computed, Single-Photon
D018955:CD36 Antigens; D005227:Fatty Acids; D007457:Iodine Radioisotopes; D007462:Iodobenzenes; D010980:Platelet Membrane Glycoproteins; C049338:iodofiltic acid