Effects of surfactants on glucosyltransferase production and in vitro sucrose-dependent colonization by Streptococcus mutans.
The presence of Tween 80 in media was associated with a significant increase in three glucosyltransferases(GTFs)(I, SI and S), especially GTF-I, produced by Streptococcus mutans strain PS14, indicating that the surfactant is a major cause of the enhanced GTF production observed in cultures in M4 medium. Lecithin and Tween 20 also enhanced GTF-I production, while Triton X-100 depressed it. At a lot concentration of 0.00125%, Tween 80 enhanced markedly only GTF-I production and its effect reached maximum at a concentration of 0.0025%. Water-insoluble glucan synthesis and artificial plaque formation (in vitro sucrose-dependent colonization) by PS14 were significantly enhanced by the addition of Tween 80 at concentrations over 0.00125%. These results suggest that surfactants might vary the cariogenic potential of Strep. mutans even at low concentrations.
Department of Operative Dentistry, Nihon University School of Dentistry at Matsudo, Chiba, Japan.
Y
Y
Tomita
T
T
Watanabe
T
T
Takeuchi
A
A
Nanbu
N
N
Shinozaki
T
T
Ikemi
K
K
Fukushima
D001422:Bacterial Adhesion / Q000187:drug effects; D003773:Dental Plaque / Q000378:metabolism / Q000382:microbiology; D005936:Glucans / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D017830:Octoxynol / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D011136:Polysorbates / Q000494:pharmacology; D012995:Solubility; D013295:Streptococcus mutans / Q000145:classification / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D013501:Surface-Active Agents / Q000494:pharmacology*
D005936:Glucans; D010713:Phosphatidylcholines; D011135:Polysaccharides, Bacterial; D011136:Polysorbates; D013501:Surface-Active Agents; D013395:Sucrose; D017830:Octoxynol; D005964:Glucosyltransferases