A simple method for synthesizing [gamma-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase.
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.
Second Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.
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PI
Bauer
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Várady
D000084:Acetate Kinase; D002606:Charcoal; D002848:Chromatography, DEAE-Cellulose; D009711:Nucleotides / Q000138:chemical synthesis*; D010755:Organophosphates; D010761:Phosphorus Radioisotopes
D009711:Nucleotides; D010755:Organophosphates; D010761:Phosphorus Radioisotopes; D002606:Charcoal; C011632:acetyl phosphate; D000084:Acetate Kinase