Liquid chromatographic determination of five benzoylurea insecticides in fruit and vegetables.
A liquid chromatographic (LC) method was developed to determine 5 benzoylureas--diflubenzuron, hexaflumuron, teflubenzuron, flufenozuron, and lufenuron--in peppers, tomatoes, eggplants, cucumbers, and oranges. Preparation of samples involve extraction with acetone and partitioning into dichloromethane-petroleum ether. A portion of this extract is cleaned up with a solid-phase extraction aminopropyl disposable column. With LC analysis using an RP-8-DB microbore column, acetonitrile-water (70 + 30, v/v) as mobile phase, and photodiode array detection at 254 nm, recovery and repeatability data were collected for the 5 benzoylureas on 4 vegetables and citrus in the range 0.04-2.0 mg/kg. Validated limits of detection and quantitation were 0.01 and 0.04 mg/kg, respectively. The method is reliable for routine analysis of vegetables and fruits.
Gamón
M
M
Laboratorio Agroalimentario, Valencia, Spain.
Saez
A
A
Pelegrí
R
R
Peris
I
I
de la Cuadra
J G
JG
Coscollá
R
R
D001562:Benzimidazoles / Q000032:analysis; D002853:Chromatography, Liquid; D005506:Food Contamination; D005638:Fruit / Q000737:chemistry*; D010573:Pesticide Residues / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014508:Urea / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
D001562:Benzimidazoles; D010573:Pesticide Residues; D014508:Urea