Arachidonic acid modulation of [3H]naloxone specific binding to rat brain opioid receptors.
Arachidonic acid effect on binding of [3H]naloxone to rat brain membranes were studied at opioid receptor subtype level. Arachidonic acid inhibited opioid receptor binding in a dose-dependent manner, both in the presence and absence of sodium. With blockage experiments it was shown that delta-opioid receptors were modulated by arachidonic acid to a greater extent than that of mu-opioid receptors. On the other hand, there was no significant difference in terms of IC50 values for arachidonic acid inhibition of [3H]naloxone binding at agonist and antagonist configuration of the receptor subtypes.
Department of Biology, Middle East Technical University, Ankara, Turkey.
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D000818:Animals; D016718:Arachidonic Acid / Q000494:pharmacology*; D001667:Binding, Competitive / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D008024:Ligands; D008297:Male; D009270:Naloxone / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000378:metabolism; D017450:Receptors, Opioid, mu / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D014316:Tritium
D008024:Ligands; D011957:Receptors, Opioid; D017465:Receptors, Opioid, delta; D017450:Receptors, Opioid, mu; D014316:Tritium; D016718:Arachidonic Acid; D009270:Naloxone; D012964:Sodium