MFR, a putative receptor mediating the fusion of macrophages.
We had previously identified a macrophage surface protein whose expression is highly induced, transient, and specific, as it is restricted to actively fusing macrophages in vitro and in vivo. This protein is recognized by monoclonal antibodies that block macrophage fusion. We have now purified this protein and cloned its corresponding cDNA. This protein belongs to the superfamily of immunoglobulins and is similar to immune antigen receptors such as the T-cell receptor, B-cell receptor, and viral receptors such as CD4. We have therefore named this protein macrophage fusion receptor (MFR). We show that the extracellular domain of MFR prevents fusion of macrophages in vitro and therefore propose that MFR belongs to the fusion machinery of macrophages. MFR is identical to SHPS-1 and BIT and is a homologue of P84, SIRPalpha, and MyD-1, all of which have been recently cloned and implicated in cell signaling and cell-cell interaction events.
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut, USA.
C
C
Saginario
H
H
Sterling
C
C
Beckers
R
R
Kobayashi
M
M
Solimena
E
E
Ullu
A
A
Vignery
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000943:Antigens, Differentiation; D001483:Base Sequence; D019556:COS Cells; D002459:Cell Fusion / Q000502:physiology*; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000235:genetics; D016676:Macrophages, Alveolar / Q000502:physiology*; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry*; D010446:Peptide Fragments / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000737:chemistry*; D011971:Receptors, Immunologic; D011994:Recombinant Proteins / Q000276:immunology; D017422:Sequence Analysis, DNA; D015398:Signal Transduction / Q000502:physiology; D014162:Transfection / Q000235:genetics
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation; D008562:Membrane Glycoproteins; D039842:Neural Cell Adhesion Molecule L1; D019006:Neural Cell Adhesion Molecules; D010446:Peptide Fragments; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; D011994:Recombinant Proteins; C501924:Sirpa protein, rat