Influence of 2-acetylaminofluorene (2-AAF) on biotransformation and lipid peroxidation in salivary glands and liver from male rats.
Salivary glands proved to be active in biotransformation. In microsomes of rat salivary glands 7-ethoxyresorufin O-deethylation (EROD) and 7-pentoxyresorufin O-depentylation (PEROD) were detectable, but with much lower activities than in the liver. Beside the well-known induction of EROD or PEROD in the liver by beta-naphthoflavone (BNF) or phenobarbital (PB), respectively, a marked rise in EROD rate of salivary glands was observed after BNF treatment. Administration of 2-AAF caused an increase in EROD rates in liver microsomes, but a decrease in microsomes of salivary glands. This decrease in EROD rate was accompanied by selective cytotoxic damages in the convoluted granulated tubules of the submandibular glands. No cytotoxic damage occurred in the submandibular glands after a combined administration of the inducer BNF and 2-AAF. This indicates relations between these toxic effects of 2-AAF and changes of 2-AAF-metabolism in BNF-induced rats, maybe in the liver and/or in the submandibular glands themselves.
Department of Prosthetic Dentistry and Dental Material Research, Friedrich Schiller University Jena, Germany.
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Jahn
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Planz
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Danz
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Müller
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D001711:Biotransformation / Q000187:drug effects; D002273:Carcinogens / Q000494:pharmacology*; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis*; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis*; D004790:Enzyme Induction; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D019324:beta-Naphthoflavone / Q000494:pharmacology
D002273:Carcinogens; D017392:Thiobarbituric Acid Reactive Substances; D019324:beta-Naphthoflavone; D015073:2-Acetylaminofluorene; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1; D005978:Glutathione