Attachment and growth of human rotaviruses RV-3 and S12/85 in Caco-2 cells depend on VP4.
Studies with human neonatal rotaviruses RV-3 and S12/85 and their reassortants showed that VP4 is a determinant of rotavirus attachment to and growth in Caco-2 cells. The binding of these viruses to MA104 and Caco-2 cells correlated with their growth ability. Virus sensitivity to trypsin and the VP4 fusion region may be implicated in these processes.
Department of Gastroenterology and Clinical Nutrition, Royal Children's Hospital, Parkville 3052, Victoria, Australia.
C D
CD
Kirkwood
R F
RF
Bishop
B S
BS
Coulson
D000595:Amino Acid Sequence; D018938:Caco-2 Cells; D002213:Capsid / Q000235:genetics / Q000502:physiology*; D036022:Capsid Proteins; D002460:Cell Line; D005814:Genes, Viral; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D012401:Rotavirus / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity*; D012400:Rotavirus Infections / Q000209:etiology / Q000821:virology; D017386:Sequence Homology, Amino Acid; D014357:Trypsin / Q000494:pharmacology
D036022:Capsid Proteins; C060339:VP4 protein, Rotavirus; D014357:Trypsin