Suppression of transformed phenotypes of Ha-ras-transformed NIH3T3 cells by caspase-2.
Biological effects of caspases-1, -2 and -3 have been investigated by transfection of these human cDNAs into activated c-Ha-ras-transformed NIH3T3 mouse fibroblasts. High expression of caspase-2 but not caspase-1 or -3 induced flat revertant cells which lost the ability of anchorage-independent growth. Notably, these revertants contained a reduced amount of Ras protein. These suggest that caspase-2 exhibited a tumor-suppressive activity by affecting the expression level of Ras protein.
School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba, 260-8670, Japan. hiwasamed.m.chiba-u.ac.jp
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Nakagawara
D016475:3T3 Cells; D000818:Animals; D020170:Caspase 1 / Q000235:genetics; D053143:Caspase 2; D053148:Caspase 3; D020169:Caspases / Q000235:genetics*; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D011905:Genes, ras; D006801:Humans; D051379:Mice; D014162:Transfection
D018076:DNA, Complementary; C505407:CASP3 protein, human; C505409:Casp3 protein, mouse; D053143:Caspase 2; D053148:Caspase 3; D020169:Caspases; D020170:Caspase 1