cDNA excision in stable retroviral cDNA transfectants is prevented by R removal.
The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.
Rosin-Arbesfeld
R
R
Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Gazit
A
A
Yaniv
A
A
D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D015870:Gene Expression; D015967:Gene Expression Regulation, Viral; D015321:Gene Rearrangement; D004860:Infectious Anemia Virus, Equine / Q000235:genetics*; D014162:Transfection
D018076:DNA, Complementary; D004279:DNA, Viral