Further characterization of the 5'-flanking promoter region of the human beta1-adrenergic receptor gene.
Further characterization of the 5'-flanking promoter region of the human beta1-adrenergic receptor (AR) gene was attempted. The transcription initiation sites, determined by the primer extension and the rapid amplification of the 5'-cDNA end, are multiple in a spanning about 30 nucleotides (-289 to -261 relative to the translation start site). There exist inverted CCAAT boxes, multiple binding sites for transcription factor Sp1 and AP-2 nearby transcription initiation sites, however, this region lacks a typical TATA box. In order to localize the regulatory region for the basal transcription of the human beta1-AR gene, a variety of 5'-flanking sequence/chloramphenicol acetyltransferase reporter gene fusion constructs was prepared and transiently expressed in HeLa cells. Functional analyses reveal negatively (-3813 to -2925 and -1772 to -796) as well as positively (-2925 to -1772) regulatory regions, in addition to the region (-796 to -87) being necessary for the basic expression of the human beta1-AR gene.
Department of Neuropsychiatry, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan.
Y
Y
Okawa
A
A
Adachi
M
M
Ino
S
S
Nagumo
H
H
Matsui
D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000032:analysis; D005786:Gene Expression Regulation; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors
D018076:DNA, Complementary; D018342:Receptors, Adrenergic, beta-1; D014157:Transcription Factors