Quinolinic acid-induced inflammation in the striatum does not impair the survival of neural allografts in the rat.
It has been suggested that inflammation related to intracerebral transplantation surgery can affect the survival of intrastriatal neural allografts. To test this hypothesis, we transplanted dissociated embryonic mesencephalic tissue from one of two rat strains, Lewis (allogeneic grafts) or Sprague-Dawley (syngeneic grafts), to the striatum of Sprague-Dawley rats. The target striatum was either intact or had received a local injection of quinolinic acid 9 days earlier, in order to induce a marked inflammation. At 6 or 12 weeks after transplantation, there was no significant difference between the different groups regarding the number of surviving grafted tyrosine hydroxylase immunoreactive neurons. However, the graft volume of both the syngeneic and allogeneic implants was significantly larger in the quinolinate-lesioned than in the intact striatum. There were dramatically increased levels of expression of major histocompatibility complex class I and II antigens, marked infiltrates of macrophages, activated microglia and astrocytes, and accumulation of large numbers of CD4 and CD8 positive T-lymphocytes in the quinolinate-lesioned striatum. In contrast, these immunological markers were much less abundant around both syngeneic and allogeneic grafts placed in intact striatum. We conclude that severe inflammation caused by quinolinic acid does not lead to rejection of intrastriatal neural allografts.
Section for Neuronal Survival, Wallenberg Neuroscience Center, Department of Physiology and Neuroscience, University of Lund, S olvegatan 17, S-223 62 Lund, Sweden. duanx003@tc.umn.edu
W M
WM
Duan
H
H
Widner
R M
RM
Cameron
P
P
Brundin
D000818:Animals; D001253:Astrocytes / Q000473:pathology; D003342:Corpus Striatum / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D004622:Embryo, Mammalian; D005260:Female; D006085:Graft Survival / Q000276:immunology*; D007150:Immunohistochemistry; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology*; D008264:Macrophages / Q000473:pathology; D017628:Microglia / Q000473:pathology; D009474:Neurons / Q000637:transplantation*; D017378:Quinolinic Acid / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D017207:Rats, Sprague-Dawley; D013601:T-Lymphocytes / Q000473:pathology; D013997:Time Factors
D017378:Quinolinic Acid