Involvement of DA1 and DA2 receptors in the gastroprotective activity of amylin in the rat.
Peripheral administration of amylin (40 microg kg-1) exerts gastroprotective effects in the reserpine-induced gastric lesions in the rat. This activity is decreased by pretreatment (30 min before) with (-)-sulpiride (0.1 mg kg-1 s.c.) or domperidone (0.1-2.5 mg kg-1 per os), dopamine DA2 antagonists. Pretreatment with SCH 23390 (0.5-4 mg kg-1 s.c.), a DA1 antagonist, at the maximal dose used, also significantly decreased the gastroprotective activity of the peptide. Amylin does not exert any gastroprotective effect in indomethacin-pretreated rats (7.5 mg kg-1 s.c., 30 min before), as well as in the aspirin-induced ulcer test (200 mg kg-1 per os at the time of amylin administration). Our data confirm that the gastroprotective effect of amylin in reserpine-induced gastric lesions involves, at least in part, the dopaminergic transmission, interfering with both the DA1 and DA2 receptor subtypes.
Clementi
G
G
Institute of Pharmacology, University of Catania School of Medicine, viale Andrea Doria 6, Catania, 95125, Italy.
Caruso
A
A
Cutuli
V M
VM
De Bernardis
E
E
Prato
A
A
Amico-Roxas
M
M
D000682:Amyloid / Q000494:pharmacology*; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology*; D001552:Benzazepines / Q000494:pharmacology; D004294:Domperidone / Q000494:pharmacology; D005753:Gastric Mucosa / Q000187:drug effects*; D058228:Islet Amyloid Polypeptide; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017447:Receptors, Dopamine D1 / Q000187:drug effects / Q000502:physiology*; D017448:Receptors, Dopamine D2 / Q000187:drug effects / Q000502:physiology*; D012110:Reserpine / Q000633:toxicity; D013469:Sulpiride / Q000494:pharmacology
D000682:Amyloid; D000897:Anti-Ulcer Agents; D001552:Benzazepines; D058228:Islet Amyloid Polypeptide; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D004294:Domperidone; D013469:Sulpiride; D012110:Reserpine