Cytochemical demonstration of sites of hydrogen peroxide generation and increased vascular permeability in isolated pig hearts after ischaemia and reperfusion.
Isolated pig hearts, subsequently perfused with pig or human blood, were prepared for the cytochemical demonstration of sites of hydrogen peroxide generation and increased vascular permeability. Oxidant stress was associated with ultrastructural changes commonly seen following myocardial reperfusion. In addition, the precipitation of cerium perhydroxide following perfusion with physiological saline containing cerium chloride suggested the vascular endothelium and leukocytes as sources of oxidants. This was associated with rapid penetration of horseradish peroxidase through the intercellular clefts of the vascular endothelium into the interstitial space, suggesting increased vascular leakiness at these sites. The rapid penetration of horseradish peroxidase was observed at all monitored periods of reperfusion with pig or human blood. This indicates that the increased permeability occurred during the ischaemic period and continued during reperfusion. Morphological damage was greatest in pig hearts reperfused with whole human blood and this was attenuated if the blood was preabsorbed to remove antibodies prior to reperfusion. We conclude that oxidant stress was initiated during ischaemia and continued during reperfusion in this model.
Multi-Imaging Centre, Department of Anatomy, University of Cambridge, UK.
J N
JN
Skepper
R N
RN
Pierson
V K
VK
Young
J A
JA
Rees
J M
JM
Powell
V
V
Navaratnam
N R
NR
Cary
D N
DN
Tew
P J
PJ
Bacon
J
J
Wallwork
D J
DJ
White
D K
DK
Menon
D000818:Animals; D002199:Capillary Permeability; D002563:Cerium / Q000378:metabolism; D003331:Coronary Vessels / Q000473:pathology / Q000503:physiopathology*; D004577:Electron Probe Microanalysis; D006321:Heart / Q000503:physiopathology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008854:Microscopy, Electron; D017202:Myocardial Ischemia / Q000473:pathology / Q000503:physiopathology*; D015428:Myocardial Reperfusion Injury / Q000473:pathology / Q000503:physiopathology*; D009206:Myocardium / Q000473:pathology; D018384:Oxidative Stress; D013552:Swine
D002563:Cerium; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase