Monoclonal antibodies directed against lipopolysaccharide of Legionella pneumophila serogroup 1.
The monoclonal antibodies (MAbs) against lipopolysaccharide of virulent strain of Legionella pneumophila serogroup 1 were produced. Three most productive hybrid clones (5F4, 5F10 and 2C9) were selected from fusions of mouse myeloma cells with spleen cells from BALB/c mice, immunized with bacterial outer membrane antigens. All generated clones were IgG-secreting. The MAbs had narrow strain specificity and showed no cross-reactions with other unrelated bacterial species. These antibodies were tested in sandwich ELISA. The results suggest that the MAbs could be used for diagnostic purposes.
State Research Center for Applied Microbiology, Moscow Region, Russia.
A M
AM
Baranov
S S
SS
Vetchinin
E V
EV
Baranova
S F
SF
Biketov
E A
EA
Svetoch
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D016952:Legionella pneumophila / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides