Influence of the MexAB-OprM multidrug efflux system on quorum sensing in Pseudomonas aeruginosa.
Pseudomonas aeruginosa nalB mutants which hyperexpress the MexAB-OprM multidrug efflux system produce reduced levels of several extracellular virulence factors known to be regulated by quorum sensing. Such mutants also produce less acylated homoserine lactone autoinducer PAI-1, consistent with an observed reduction in lasI expression. These data suggest that PAI-1 is a substrate for MexAB-OprM, and its resulting exclusion from cells hyperexpressing MexAB-OprM limits PAI-1-dependent activation of lasI and the virulence genes.
Evans
K
K
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Passador
L
L
Srikumar
R
R
Tsang
E
E
Nezezon
J
J
Poole
K
K
D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000378:metabolism*; D001692:Biological Transport; D018432:Drug Resistance, Multiple / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D010675:Pheromones / Q000096:biosynthesis; D011550:Pseudomonas aeruginosa / Q000472:pathogenicity / Q000502:physiology*; D011710:Pyocyanine / Q000096:biosynthesis; D015398:Signal Transduction / Q000235:genetics; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; C081237:LasI protein, Pseudomonas aeruginosa; D010675:Pheromones; C030654:N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone; D011710:Pyocyanine; D015107:4-Butyrolactone