Preservation of plant cell ultrastructure during immunolocalization of virus particles.
Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus.
Ghoshroy
S
S
Department of Plant Sciences, University of Arizona, Tucson 85721, USA.
Citovsky
V
V
D017360:Arabidopsis / Q000648:ultrastructure / Q000821:virology; D001937:Brassica / Q000821:virology; D016253:Microscopy, Immunoelectron / Q000379:methods*; D010944:Plants / Q000648:ultrastructure* / Q000821:virology*; D010947:Plants, Toxic; D016610:Tissue Embedding; D016707:Tissue Fixation; D014026:Nicotiana / Q000648:ultrastructure / Q000821:virology; D017898:Tobamovirus / Q000302:isolation & purification* / Q000648:ultrastructure