Electrostatics of the phospholipase-membrane interaction.
The electrostatic interaction of the Phospholipase A2 (PLA2)-membrane complex in the presence and absence of calcium is analysed by the computation of the electrostatic profiles of the components and the complex. The electrostatic potential was computed by using of the program MOLPOT that implement the boundary element method to solve the electrostatic problem. It considers a closed surface in three dimensions that contains the macromolecule that follows as close as possible the macromolecule shape. The results show that the presence of calcium ions contributes to the stability of the complex and at the same time creates a favourable electrostatic potential pattern that may be favourable for the lipolysis of the membrane components.
Costabel
M D
MD
Departamento de Física, Universidad Nacional del Sur, Bahia Blanca, Argentina.
Vallejo
D F
DF
Grigera
J R
JR
D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D003198:Computer Simulation; D008564:Membrane Potentials; D008956:Models, Chemical; D010741:Phospholipases A / Q000737:chemistry* / Q000378:metabolism*; D054467:Phospholipases A2; D055672:Static Electricity
D010741:Phospholipases A; D054467:Phospholipases A2; D002118:Calcium