Oral microbiota and implant type membranes.
Candida albicans (Ca), Staphylococcus aureus (Sa), Streptococcus sanguis (Ss), Actinomyces naeslundii (An), Actinomyces odontolyticus (Ao), Porphyromona spp (P spp), Candida glabrata (Cg), Candida krusei (Ck), and Rhodotorula spp (R spp) were tested with equal pieces of biodegradable membranes. Membranes pretreated with saliva or clorhexidine and nontreated control membranes were tested in three different culture media containing 0.1 mL homologous suspension for each strain under study. Incubation was performed at 37 degrees C for 48 hours for aerobiosis and for five days for anaerobiosis. Macroscopy and microscopy were carried out. Membranes were removed, washed, and resuspended. Samples were sonicated, and the supernatant was disseminated on brain heart infusion broth or blood agar. Incubation was repeated, colony-forming unit counts were performed, and statistical analysis was carried out using analysis of variance transforming results to Log10 (x + 1), the highest interaction level was used to calculate standard error. Orthogonal contrast was used to compare the different microorganisms under study. Highest adhesion was found with Ca, Cg, Ck, Sa, and Ss. A sufficient quantity of Actinomyces could not be recovered from the membranes. Results with P spp were poor, confirming lower gram-negative adhesion. Replicate flasks with Ss and Ca were cultivated. Membranes were removed after washing and subjected to scanning electron microscopy, as were untreated control pieces. A cavelike surface was observed. Streptococcus sanguis adhering to the membranes showed extracellular projections. Candida and gram-positive cocci showed great recovery capacity.
Microbiology Department, School of Dentistry, University of Buenos Aires, Argentina.
S L
SL
Molgatini
M I
MI
González
A C
AC
Rosa
M B
MB
Negroni
D000190:Actinomyces / Q000254:growth & development / Q000502:physiology; D000704:Analysis of Variance; D001422:Bacterial Adhesion; D001673:Biodegradation, Environmental; D018441:Biofilms / Q000254:growth & development*; D002175:Candida / Q000254:growth & development / Q000502:physiology; D015169:Colony Count, Microbial; D017077:Culture Media, Conditioned; D015921:Dental Implants / Q000382:microbiology*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D009055:Mouth / Q000382:microbiology*; D017861:Porphyromonas / Q000254:growth & development / Q000502:physiology; D012248:Rhodotorula / Q000254:growth & development / Q000502:physiology; D013210:Staphylococcus / Q000254:growth & development / Q000502:physiology; D013291:Streptococcus / Q000254:growth & development / Q000502:physiology
D017077:Culture Media, Conditioned; D015921:Dental Implants; D008567:Membranes, Artificial