Expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes in squamous cell carcinomas of the cervix uteri and metastases.
Monocyte chemoattractant protein 1 (MCP-1) plays an important role in the recruitment of monocytes in solid tumors. Previously, for cervical carcinoma cell lines an inverse correlation between the expression of the MCP-1 gene and the human papillomavirus (HPV) oncogenes E6/E7 was described. In this study paraffin-embedded biopsy specimens from 25 squamous cell carcinomas of the cervix uteri were analyzed for the expression of the MCP-1 gene and the HPV 16 E6/E7 oncogenes by RNA/RNA in situ hybridization. Similar to in vitro analyses, in our material a negative correlation between the transcription of these genes in tumor cells could be demonstrated in 19/25 cases, whereas in 6 cases both MCP-1 gene and HPV oncogene transcription could be detected. Interestingly, the same results regarding MCP-1 gene and HPV oncogene expression were observed in the majority of corresponding metastases. On the other hand, in stromal cells MCP-1-specific transcripts could be detected in all cases. In most of them intra- and/or peritumoral macrophages were observed. Our findings support the hypothesis that HPV oncoproteins are negative regulators of MCP-1 transcription.
Department of Gynecopathology, University Hospital Hamburg, Germany. riethdor@plexus.uke.uni-hamburg.de
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Riethdorf
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Riethdorf
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Richter
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Löning
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000556:secondary / Q000821:virology*; D018932:Chemokine CCL2 / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D005814:Genes, Viral; D006801:Humans; D009856:Oncogene Proteins, Viral / Q000235:genetics*; D009857:Oncogenes; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D050725:Papillomavirus E7 Proteins; D030361:Papillomavirus Infections / Q000150:complications / Q000235:genetics / Q000821:virology; D012097:Repressor Proteins; D014412:Tumor Virus Infections / Q000150:complications / Q000235:genetics / Q000821:virology; D002583:Uterine Cervical Neoplasms / Q000150:complications / Q000235:genetics* / Q000821:virology*
D018932:Chemokine CCL2; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D050725:Papillomavirus E7 Proteins; D012097:Repressor Proteins; C059731:oncogene protein E7, Human papillomavirus type 16