Motif 2 in adenosine kinase homologous ginseng polypeptide showed affinity to D-ribose by capillary zone electrophoresis and surface plasmon resonance.
Affinity capillary electrophoresis (ACE) and surface plasmon resonance (SPR) were applied to the analysis of anti-lipolytic acidic tetradecapeptide from Panax ginseng roots. The ginseng polypeptide (GPP) and modified GPPs were chemically synthesized and their affinity to D-ribose and adenosine was examined by ACE and SPR. GPP had affinity to D-ribose and adenosine and the binding constants (Kb) to GPP were calculated by both methods (Kb = 1.04 x 10(4) mol-1 to D-ribose by ACE and Kb = 1.91 x 10(4) mol-1 to adenosine by SPR). Most of the modified GPPs lost their affinity to D-ribose and adenosine through substitution or rearrangement of the amino acids.
National Institute of Agrobiological Resources, Ibaraki, Japan.
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Kajiwara
D000248:Adenosine Kinase / Q000737:chemistry*; D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D004837:Epinephrine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000960:Hypolipidemic Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D008066:Lipolysis / Q000187:drug effects; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D012266:Ribose / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013057:Spectrum Analysis
D000960:Hypolipidemic Agents; D010940:Plant Proteins; C104839:ginseng pentadecapeptide, Panax ginseng; D012266:Ribose; D000248:Adenosine Kinase; D004837:Epinephrine