Isolation of a novel mycobacterium from an adolescent with cervical lymphadenitis.
A slow-growing mycobacterium was isolated from a cervical lymph node of an adolescent male. This isolate produced small, smooth, scotochromogenic colonies after 6 weeks of incubation at 25 degrees C and 30 degrees C (but not at 37 degrees C or 43 degrees C). The results of 16S-rRNA gene sequencing and high-performance liquid chromatography suggest that this isolate belongs to a hitherto unrecognised pathogenic species.
Goodwin
A
A
Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia.
Lumb
R
R
Patkin
M
M
Bastian
I
I
D000293:Adolescent; D015373:Bacterial Typing Techniques; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D008198:Lymph Nodes / Q000382:microbiology; D008297:Male; D008969:Molecular Sequence Data; D009161:Mycobacterium / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D009164:Mycobacterium Infections / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014388:Tuberculosis, Lymph Node / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S