[Relationship between methylenetetrahydrofolate reductase gene polymorphism and coronary heart disease].
OBJECTIVE
This study inquired into the relationship between methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and coronary heart disease.


METHODS
By polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), MTHFR 677C-->T mutation was detected in 79 healthy controls and 69 patients with coronary heart disease.


RESULTS
The frequency of MTHFR variant V677 of patients was significantly higher than that of healthy controls (P<0.01).


CONCLUSIONS
This study demonstrated that MTHFR gene V677 mutation was probably one of the genetic risk factors of coronary heart disease and this provided a new basis for exploring the relevant pathogenesis.
Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang, 150086 P. R. China. biology@ems.hrbmu.edu.cn
B
B
Chen
X
X
Wang
J
J
Yu
G
G
Zhang
F
F
Chen
J
J
Den
S
S
Fu
P
P
Li
W
W
Cao
H
H
Yu
D003327:Coronary Disease / Q000235:genetics*; D006801:Humans; D042965:Methylenetetrahydrofolate Reductase (NADPH2); D009154:Mutation; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors
D000587:Oxidoreductases Acting on CH-NH Group Donors; D042965:Methylenetetrahydrofolate Reductase (NADPH2)