Bioactive factors affect proliferation and phenotypic expression in progenitor and pluripotent stem cells.
Progenitor and pluripotent stem cells reside within connective tissue compartments. They are also present in granulation tissue. This study examined the effects of treating these two cell populations with eight bioactive factors. Cells were assayed for DNA content as a measure of proliferation and for tissue-specific phenotypic markers as measures of lineage progression and lineage commitment. Platelet-derived endothelial growth factor and insulin-like growth factor-II did not induce proliferation in either population. However, dexamethasone, insulin, insulin-like growth factor-I, muscle morphogenetic protein, platelet-derived growth factor-AA, and platelet-derived growth factor-BB stimulated proliferation in one or both cell populations. Platelet-derived growth factor-BB was the most potent stimulator of proliferation in either population. Phenotypic expression markers were induced in the progenitor cells by insulin, insulin-like growth factor-I, insulin-like growth factor-II, dexamethasone, and muscle morphogenetic protein. However, only dexamethasone and muscle morphogenetic protein induced phenotypic expression markers in the pluripotent cells. Platelet-derived endothelial cell growth factor, platelet-derived growth factor-AA, and platelet-derived growth factor-BB did not induce phenotypic expression markers in progenitor or pluripotent cells. This study suggests the potential for using progenitor and pluripotent cells as an in vitro model to ascertain the effects of various bioactive factors on stem cells potentially involved in tissue maintenance and repair.
Division of Basic Medical Sciences, Mercer University School of Medicine, Macon, Ga, USA.
H E
HE
Young
R P
RP
Wright
M L
ML
Mancini
P A
PA
Lucas
C R
CR
Reagan
A C
AC
Black
D000818:Animals; D002455:Cell Division / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D004247:DNA / Q000032:analysis / Q000187:drug effects; D003907:Dexamethasone / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D007334:Insulin-Like Growth Factor I / Q000378:metabolism* / Q000494:pharmacology; D009124:Muscle Proteins / Q000378:metabolism / Q000494:pharmacology; D010641:Phenotype; D010982:Platelet-Derived Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D012016:Reference Values; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D013939:Thymidine Phosphorylase / Q000378:metabolism* / Q000494:pharmacology
D003470:Culture Media; D007328:Insulin; D009124:Muscle Proteins; D010982:Platelet-Derived Growth Factor; D007334:Insulin-Like Growth Factor I; D003907:Dexamethasone; D004247:DNA; D013939:Thymidine Phosphorylase