Substrate specificity of the human coronavirus 229E 3C-like proteinase.
Coronavirus gene expression involves proteolytic processing of the gene 1-encoded polyproteins and a key enzyme in this process is the virus-encoded 3C-like proteinase. In this study, we describe the biosynthesis of the human coronavirus 229E 3C-like proteinase in Escherichia coli and the substrate specificity of the purified protein. Using immunofluorescence microscopy, we have also investigated the subcellular localization of the 3C-like proteinase and have found a punctate, perinuclear distribution of the proteinase in virus-infected cells.
Institute of Virology, University of Würzburg, Germany.
J
J
Ziebuhr
G
G
Heusipp
A
A
Seybert
S G
SG
Siddell
D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D002460:Cell Line; D017934:Coronavirus / Q000201:enzymology*; D028941:Coronavirus 229E, Human; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014764:Viral Proteins
D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases