[Tyrosine kinases of the Src family, enzymes with multiple functions: from the growth of fibroblasts to the migration of epithelial cells].
The tyrosine kinases of the Src family were first discovered due to their oncogenic properties. In untransformed fibroblasts, these kinases are activated as cells exists quiescence in response to some growth factors. Using microinjection to introduce catalytically inactive dominant-negative form of cSrc, as well as an antibody that neutralizes cSrc, Fyn and cYes, we have shown that Src kinases are required for DNA synthesis induced by most growth factors (PDGF, EGF, CSF-1, insulin, IGF-1). A functional link between Src kinases and the expression of the transcription factor c-Myc was also shown. In addition to cell growth promotion, some factors induce epithelial cell scattering and this also requires cSrc and cYes activities. However, in contrast to mitogenesis, they do not need novel gene expression for signalling but rather may act by phosphorylating components that regulate the cytoskeleton. Finally, increased Src kinase activities were found in several human carcinomas and we propose that these enzymes are involved in cell invasion.
Roche
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CNRS EP 612, Faculté de Pharmacie, Montpellier. roche@balard.pharma.univ-montp1.fr
D000818:Animals; D002465:Cell Movement; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells / Q000502:physiology*; D005347:Fibroblasts / Q000166:cytology*; D006133:Growth Substances / Q000494:pharmacology / Q000502:physiology; D006801:Humans; D009361:Neoplasm Invasiveness; D009369:Neoplasms / Q000201:enzymology / Q000473:pathology; D011505:Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction; D018909:src Homology Domains
D006133:Growth Substances; D004247:DNA; D011505:Protein-Tyrosine Kinases
Tyrosine kinases de la famille Src, enzymes aux multiples fonctions: de la croissance des fibroblastes à la migration des cellules épithéliales.