A composite CMV-IE enhancer/beta-actin promoter is ubiquitously expressed in mouse cutaneous epithelium.
In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable to express a transgene in all the cells of the cutaneous epithelium. Since no ubiquitously expressed promoter sequences had previously been identified, we used lacZ reporter transgenes to test two enhancer/promoter sequences for ubiquitous expression in the skin of adult transgenic mice. We find that a CMV enhancer/CMV promoter is not active in most cell types in the skin, whereas a CMV enhancer/modified beta-actin promoter sequence is active in the suprabasal and basal cells of the epidermis as well as in the epithelial cells of the hair follicles, sebaceous glands, and the dermal papillae.
Lankenau Medical Research Center, 100 Lancaster Avenue, Wynnewood, Pennsylvania, 19096, USA.
J A
JA
Sawicki
R J
RJ
Morris
B
B
Monks
K
K
Sakai
J
J
Miyazaki
D000199:Actins / Q000235:genetics*; D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics*; D002645:Chickens; D003433:Crosses, Genetic; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D004847:Epithelial Cells / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012867:Skin / Q000378:metabolism*
D000199:Actins; D000956:Antigens, Viral; D017874:Immediate-Early Proteins; C044582:immediate-early proteins, cytomegalovirus