The effects of nitric oxide on the contractility of isolated uterine and aortic rings from pregnant rats.
OBJECTIVE
The object was to compare the effects of nitric oxide on isolated uterus and aorta of pregnant rats.


METHODS
Rings of uterus and thoracic aorta without endothelium from Sprague-Dawley rats at mid and late gestation were used for isometric tension recording. The concentration-response curve for diethylamine/nitric oxide was studied in the presence or absence of oxyhemoglobin (10(-5) mol/L), or oxyhemoglobin was added after the response to diethylamine/nitric oxide.


RESULTS
Diethylamine/nitric oxide concentration dependently inhibited uterine contractions, and the effect was attenuated by previous treatment with oxyhemoglobin at mid gestation (n = 8). The effects were negligible at late gestation (n = 8). The relaxation of aortic rings by diethylamine/nitric oxide and its attenuation by previous treatment with oxyhemoglobin were similar at mid (n = 6) and late (n = 6) gestation. The sensitivity of aortic rings to diethylamine/nitric oxide is significantly higher than that of uterine rings. Oxyhemoglobin partly restored inhibited diethylamine/nitric oxide phenylephrine tension in aortic rings and had no effect on diethylamine/nitric oxide-inhibited uterine rings.


CONCLUSIONS
Uterine smooth muscle is less sensitive to nitric oxide than is aortic smooth muscle. Nitric oxide sensitivity of rat uterus but not aorta decreases toward term.
University of Texas Medical Branch, Galveston 77555-1062, USA.
T
T
Okawa
A S
AS
Syal
Y P
YP
Vedernikov
G R
GR
Saade
K
K
Chwalisz
R E
RE
Garfield
D000818:Animals; D001011:Aorta / Q000187:drug effects*; D004048:Diethylamines / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D009569:Nitric Oxide / Q000494:pharmacology*; D010108:Oxyhemoglobins / Q000494:pharmacology; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014590:Uterine Contraction / Q000187:drug effects*; D014661:Vasoconstriction / Q000187:drug effects*
D004048:Diethylamines; D010108:Oxyhemoglobins; D009569:Nitric Oxide; C034281:diethylamine