Positive and negative control of Serrate expression during early development of the Drosophila wing.
The product of the Drosophila gene Serrate acts as a short-range signal during wing development to induce the organising centre at the dorsal/ventral compartment boundary, from which growth and patterning of the wing is controlled. Regulatory elements reflecting the early Serrate expression in the dorsal compartment of the wing disc have recently been confined to a genomic fragment in the 5'-upstream region of the gene. Here we present data to suggest that this fragment responds to various positive and negative inputs required for the early Serrate expression. First, activation and maintenance of expression in the dorsal compartment of the wing discs of second and early third instar larvae depends on apterous, as revealed by reporter gene expression in discs either lacking or ectopically expressing apterous. Second, transcriptional downregulation during third larval instar is mediated by hiiragi. Finally, this regulatory element responds to Delta signalling in a nonautonomous way to maintain Serrate expression along the dorsal margin. The results clearly show that some of the previously described transactivators of Serrate protein expression, e.g. fringe, act on elements required for later aspects of Serrate expression.
Institut für Genetik, Heinrich-Heine Universität Düsseldorf, Universitätsstr. 1, 40225, Düsseldorf, Germany.
A
A
Bachmann
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Knust
D000818:Animals; D002135:Calcium-Binding Proteins; D015536:Down-Regulation; D004330:Drosophila / Q000235:genetics / Q000254:growth & development*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter; D018398:Homeodomain Proteins; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D060850:LIM-Homeodomain Proteins; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D014921:Wings, Animal / Q000254:growth & development*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D002135:Calcium-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D036341:Intercellular Signaling Peptides and Proteins; D047908:Intracellular Signaling Peptides and Proteins; D000072100:Jagged-1 Protein; D060850:LIM-Homeodomain Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C000606278:Ser protein, Drosophila; D000072099:Serrate-Jagged Proteins; D014157:Transcription Factors; C074459:ap protein, Drosophila; C064045:delta protein; D017351:N-Acetylglucosaminyltransferases; C090576:fng protein, Drosophila; D001616:beta-Galactosidase