Quantification of penicillin-G and procaine in equine urine and plasma using high-performance liquid chromatography.
A rapid and sensitive method for the extraction and quantification of penicillin-G and procaine in horse urine and plasma samples has been successfully developed. The method involves the use of solid-phase extraction (SPE) for penicillin-G, liquid-liquid extraction (LLE) for procaine, and high-performance liquid chromatography (HPLC) for the quantification of penicillin-G and procaine. The new method described here has been successfully applied in the pharmacokinetic studies of procaine, penicillin-G and procaine-penicillin-G administrations in the horse.
Luo
Y
Y
University of Pennsylvania, School of Veterinary Medicine, New Bolton Center Campus, Philadelphia 19348, USA.
McNamara
B
B
Fennell
M A
MA
Teleis
D C
DC
May
L
L
Rudy
J
J
Watson
A O
AO
Uboh
C E
CE
Soma
L R
LR
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006736:Horses; D010400:Penicillin G / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D011343:Procaine / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D011343:Procaine; D010400:Penicillin G