Immuno-stimulatory effects of bacterial-derived plasmids depend on the nature of the antigen in intramuscular DNA inoculations.
The CpG motifs of bacterial-derived plasmids augment antigen-specific immune responses and steer those responses towards the T helper 1 (Th1) type. In this study, we have addressed the immuno-stimulatory effect of intramuscular co-administration of CpG motifs containing vector DNA on the modulation of immune responses to the haemagglutinin (HA) and the nucleoprotein (NP) proteins of influenza virus. The co-administration of vector DNA with a HA-encoding plasmid DNA showed a significant enhancement in the total IgG response, the generation of cytotoxic T lymphocyte (CTL), and the T-cell proliferative response. In the case of NP-encoding plasmid DNA inoculations, the co-administration of vector DNA slightly decreased the total IgG response, although the IgG2a/IgG1 ratio and the CTL responses to NP were significantly increased. These observations suggest that the immuno-stimulatory effects of bacterial-derived plasmids depend upon the nature of the co-administered antigen.
Lee
S W
SW
Department of Life Science, School of Environmental Engineering, Pohang University of Science and Technology, Hyoja Dong, Phoang, Korea.
Sung
Y C
YC
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage*; D001419:Bacteria / Q000235:genetics*; D005260:Female; D005822:Genetic Vectors; D006389:Hemagglutinins, Viral / Q000235:genetics; D007111:Immunity, Cellular; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology*; D007273:Injections, Intramuscular; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009698:Nucleoproteins / Q000235:genetics; D010957:Plasmids / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G; D009698:Nucleoproteins; D019444:Vaccines, DNA