An antimicrobial activity of cytolytic T cells mediated by granulysin.
Cytolytic T lymphocytes (CTLs) kill intracellular pathogens by a granule-dependent mechanism. Granulysin, a protein found in granules of CTLs, reduced the viability of a broad spectrum of pathogenic bacteria, fungi, and parasites in vitro. Granulysin directly killed extracellular Mycobacterium tuberculosis, altering the membrane integrity of the bacillus, and, in combination with perforin, decreased the viability of intracellular M. tuberculosis. The ability of CTLs to kill intracellular M. tuberculosis was dependent on the presence of granulysin in cytotoxic granules, defining a mechanism by which T cells directly contribute to immunity against intracellular pathogens.
Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA.
S
S
Stenger
D A
DA
Hanson
R
R
Teitelbaum
P
P
Dewan
K R
KR
Niazi
C J
CJ
Froelich
T
T
Ganz
S
S
Thoma-Uszynski
A
A
Melián
C
C
Bogdan
S A
SA
Porcelli
B R
BR
Bloom
A M
AM
Krensky
R L
RL
Modlin
D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D003594:Cytoplasmic Granules / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006801:Humans; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D008562:Membrane Glycoproteins / Q000276:immunology / Q000494:pharmacology; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000502:physiology / Q000648:ultrastructure; D054353:Perforin; D052899:Pore Forming Cytotoxic Proteins; D011994:Recombinant Proteins / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*
D000945:Antigens, Differentiation, T-Lymphocyte; C075005:GNLY protein, human; D008562:Membrane Glycoproteins; D052899:Pore Forming Cytotoxic Proteins; D011994:Recombinant Proteins; D054353:Perforin