A vector for the expression of recombinant monoclonal Fab fragments in bacteria.
The availability of genes coding for monoclonal Fab fragments of a desired specificity permits their expression in bacteria and provides a simple method for the generation of good quality reagents. In this paper we describe a new phagemid vector for the production of recombinant Fabs from genes obtained from phage display combinatorial libraries. The phagemid features an antibiotic resistance cassette which, once inserted between the heavy chain fragment and the light chain genes, avoids unwanted recombination and preserves useful restriction sites not affecting the Fab production rate.
Burioni
R
R
Istituto di Microbiologia, Università Cattolica del S. Cuore, Rome, Italy. r.burioni@agora.stm.it
Plaisant
P
P
Bugli
F
F
Delli Carri
V
V
Clementi
M
M
Fadda
G
G
D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D002702:Chloramphenicol Resistance / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific; D004926:Escherichia coli; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000096:biosynthesis* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D007140:Immunoglobulin Fab Fragments; D011993:Recombinant Fusion Proteins; C067043:endodeoxyribonuclease NheI; C056436:endodeoxyribonuclease SpeI; D015252:Deoxyribonucleases, Type II Site-Specific