Total parenteral nutrition supplemented with medium-chain triacylglycerols prevents atrophy of the intestinal mucosa in septic rats.
Total parenteral nutrition (TPN) is associated with an increased incidence of bacterial translocation (BT) compared with enteral nutrition because of the disuse atrophy of the intestine. In this study, we assessed the effect of adding medium-chain triacylglycerols (MCT) to TPN for the prevention of mucosal atrophy in the intestine. Rats were subjected to either fat-free TPN, TPN with long-chain triacylglycerols (LCT), or TPN with MCT for 5 d and nutrition parameters were evaluated. In another set of rats receiving the same TPN regimen, 0.8 or 0.05 mg/kg endotoxin was administered on day 4. Survival was evaluated and BT to the mesenteric lymph nodes, liver, and systemic blood was measured 24 h later. The mucosal heights of the jejunum and ileum were evaluated concurrently. The survival rate was significantly improved in the MCT group (P < 0.05) at the endotoxin dose of 0.8 mg/kg. The nutrition condition presented by phospholipid, total cholesterol, and total ketone body levels was the best in the MCT group compared to the other groups. The intestinal villous height in the ileum was significantly greater in the MCT group. However, the improvement of BT in MCT group was not statistically significant. In this endotoxin-challenged rat model, survival rate was improved by the supplementation of MCT. This effect may be presented in some part by the improvement in nutrition condition and by the prevention of mucosal atrophy in the intestine.
Iba
T
T
Department of Surgery, Juntendo Urayasu Hospital, Juntendo University School of Medicine, Chiba, Japan. LDW06754@niftyserve.or.jp
Yagi
Y
Y
Kidokoro
A
A
Ohno
Y
Y
Kaneshiro
Y
Y
Akiyama
T
T
D000818:Animals; D001284:Atrophy / Q000517:prevention & control; D018988:Bacterial Translocation; D002210:Caprylates / Q000008:administration & dosage; D004926:Escherichia coli; D005230:Fatty Acids, Nonesterified / Q000097:blood; D007413:Intestinal Mucosa / Q000473:pathology*; D008070:Lipopolysaccharides; D008297:Male; D009752:Nutritional Status; D010289:Parenteral Nutrition, Total / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D015996:Survival Rate; D014280:Triglycerides / Q000008:administration & dosage* / Q000097:blood
D002210:Caprylates; D005230:Fatty Acids, Nonesterified; D008070:Lipopolysaccharides; D014280:Triglycerides; C003637:tricaprylin