Isolation, replication and polyhedrin gene sequence of an Israeli Helicoverpa armigera single nucleopolyhedrovirus.
A local strain of Helicoverpa armigera baculovirus was isolated from infected H. armigera larvae. Infectivity to Helicoverpa cells, restriction enzyme analysis and electron microscopy allowed its identification as a single embedded nucleopolyhedrovirus, designated HaSNPV-IS. Analysis of DNA replication, protein synthesis and polyhedrin expression in HaSNPV-infected cells located the late and very late phases of the viral cycle at 24 and 48 h after infection, respectively. The viral polyhedrin gene was isolated and characterized. It encoded for a polypeptide of 246 amino acid residues. A 32 kDa polypeptide was identified by immunoblot analysis using antipolyhedrin antiserum. The HaSNPV-IS polyhedrin DNA sequence revealed 99.4% of homology to the HzSNPV polyhedrin. The availability of this efficient replication system and the above knowledge paves the way to future genetic engineering of the HaSNPV.
Rivkin
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Entomology Department, Volcani Center, Bet Dagan, Israel.
Mor
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Chejanovsky
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005814:Genes, Viral / Q000235:genetics*; D007557:Israel; D008969:Molecular Sequence Data; D009036:Moths / Q000821:virology*; D019251:Nucleocapsid; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D000077183:Occlusion Body Matrix Proteins; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics*; D015678:Viral Structural Proteins / Q000235:genetics*; D014779:Virus Replication
D004279:DNA, Viral; D000077183:Occlusion Body Matrix Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus