[Effect of aconitine on content of corticotropin-releasing hormone in hypothalamus of rats].
The effect of aconitine on the content of corticotropin-Releasing-Hormone (CRH) in the hypothalamus of the normal rat injected with aconitine intraperitoneally was studied by means of radioimmunoassay (RIA). Aconitine is the main effective component of Radix Aconiti Carmichaeli Praeparata, the representative traditional Chinese herbal medicine of Warm-Supplementing Kidney-Yang (WSKY). Results showed that the content of hypothalamic CRH menifested a dosedependent increase after 7 days intraperitoneal injection of aconitine (1 microgram/kg, 3 micrograms/kg and 10 micrograms/kg). The CRH neurons in the paraventricular nucleus of hypothalamus and the neural fibers of median eminence also demonstrated increases in number and enrichment in gray tone as observed by immunohistochemical method of ABC. These results suggested that one of the possible mechanism of the WSKY drugs in improving the HPA axis might be exciting the hypothalamic CRH neurons.
Cai
D F
DF
Huashan Hospital, Shanghai Medical University.
Shen
Z Y
ZY
Chen
X H
XH
D000157:Aconitine / Q000494:pharmacology*; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D007031:Hypothalamus / Q000378:metabolism*; D007274:Injections, Intraperitoneal; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D003346:Corticotropin-Releasing Hormone; D000157:Aconitine