Abnormal DNA damage-inducible protein in cells from Sjögren's syndrome patients.
Antinuclear antibodies are commonly found in patients with Sjögren's syndrome. It has been suggested that the development of antinuclear antibodies depends on the activation of the spliceosome and other transcription-related subcellular particles, some of which have recently been shown also to function in DNA-modifying processes, such as DNA repair and V(D)J recombination. These observations add weight to a previously proposed model for the aetiology of Sjögren's syndrome. This includes the abnormal processing of the T-cell receptor and immunoglobulin genes. To test this hypothesis further, the present study on DNA-modifying proteins in Sjögren's syndrome was initiated. Gel-shift experiments using protein extracted from UV-treated Sjögren cells provided evidence of high molecular weight DNA-binding protein in six out of 12 Sjögren patients studied (but not among seven healthy controls). Some Sjögren sera displayed antibodies to protein extracts from cells treated with psoralen plus UVA radiation. These results indicate an abnormal DNA damage-inducible response in Sjögren's syndrome. It may therefore be concluded that alterations in nuclear protein may play a role in the aetiology of Sjögren's syndrome.
Department of Medical Microbiology, Lund University, University Hospital, Malmö, S-205 02, Sweden.
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S
Lavasani
G
G
Henriksson
M
M
Brant
A
A
Henriksson
M
M
Radulic
R
R
Manthorpe
A
A
Bredberg
D000974:Antibodies, Antinuclear / Q000097:blood; D034961:Antigens, Nuclear; D001323:Autoantibodies / Q000097:blood; D001402:B-Lymphocytes / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D004249:DNA Damage; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000032:analysis / Q000097:blood*; D005363:Ficusin / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015151:Immunoblotting; D000072200:Ku Autoantigen; D009687:Nuclear Proteins / Q000032:analysis; D017319:Photosensitizing Agents / Q000494:pharmacology; D012859:Sjogren's Syndrome / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D014466:Ultraviolet Rays
D000974:Antibodies, Antinuclear; D034961:Antigens, Nuclear; D001323:Autoantibodies; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D017319:Photosensitizing Agents; D004265:DNA Helicases; C106893:XRCC5 protein, human; C000606509:Xrcc6 protein, human; D000072200:Ku Autoantigen; D005363:Ficusin