Expression of VCAM-1 in lymphocytes during the process of apoptosis.
The administration of corticosteroids induced apoptosis of thymocytes in vivo. Among various adhesion molecules examined, vascular cell adhesion molecule-1 (VCAM-1, CD106) was shown to be strongly expressed in these apoptotic cells. Flow cytometric analysis also showed the expression of VCAM-1 in apoptotic thymocytes. An RT-PCR study demonstrated the expression of VCAM-1 mRNA in thymocytes. Splenic lymphocytes and other lymphoid cell lines also expressed VCAM-1 during the process of apoptosis. VCAM-1 mRNA expression was also observed in RT-PCR performed on these cell lines.
Department of Pathology and Immunology, Faculty of Medicine, Tokyo Medical and Dental University, Tokyo, Japan. ishipth2@med.tmd.ac.jp
N
N
Ishiyama
M
M
Kitagawa
H
H
Takahashi
T
T
Kina
K
K
Hirokawa
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D005434:Flow Cytometry; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006854:Hydrocortisone / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D007700:Kinetics; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics*
D017931:DNA Primers; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; D006854:Hydrocortisone