Insertion of the IL-2 gene decreases tumorigenicity of murine fibrosarcoma.
Murine interleukin 2 (mIL-2) cDNA was introduced through lipofection into cells of murine F-69-3 fibrosarcoma line established in vitro from tumors induced chemically in athymic mice. Using a modified MTT bioassay in CTLL-2 indicator line the F-69-3/IL-2 transfectants were found to secrete between 650-1750 laboratory units (LU) of IL-2/5 x 10(5) cells/48 h in restricted culture conditions. When inoculated subcutaneously to immunocompetent BALB/c or CD2F1 mice, the transfected cells showed reduced tumorigenic potential as compared with parental F-69-3/wt or control F-69-3/neo cells. The rejection of F-69-3/IL-2 tumors required an intact immune system as they grew progressively in athymic mice. The majority of immunocompetent mice that rejected IL-2 secretors were found to be protected against subsequent challenge with parental cells. These preliminary results suggest that IL-2-transfected murine fibrosarcoma could be used as a model for studying mechanisms underlying the antitumor immune response.
Department of Tumor Immunology, Polish Academy of Sciences, Wrocław, Poland.
H
H
Kuśnierczyk
P
P
Załecki
E
E
Pajtasz-Piasecka
C
C
Radzikowski
D000818:Animals; D018076:DNA, Complementary / Q000008:administration & dosage*; D005354:Fibrosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D015316:Genetic Therapy; D007376:Interleukin-2 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D007376:Interleukin-2