Kinetics of activation of a Ca2+-dependent K+ current induced by flash photolysis of caged carbachol in isolated guinea-pig outer hair cells.
It has been shown that the application of acetylcholine activates a Ca2+-dependent K+ current in outer hair cells, and the resulting hyperpolarization is thought to be an important part of the inhibition mediated by cholinergic efferent nerve fibres to the cochlea. In order to study the kinetics of the current, flash photolysis has been used to apply a cholinergic agonist, carbachol, rapidly to isolated outer hair cells. A delay in the onset of the outward potassium current following photorelease of carbachol was consistently observed, and the activation phase of the response could be described by a sigmoidal-like function with a mean delay of 59 ms and time constant of 71 ms. The sum of these values lies within the time scale reported for the onset of the inhibition following electrical stimulation of the efferent nerves. Although a distinct current attributable to an acetylcholine receptor was not visible in these experiments, indirect evidence for a carbachol-induced influx of Ca2+ was obtained.
Department of Communication and Neuroscience, Keele University, Staffordshire, UK.
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D000818:Animals; D002038:Bungarotoxins / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002217:Carbachol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000528:radiation effects; D002478:Cells, Cultured; D005463:Fluoroacetates; D006168:Guinea Pigs; D018072:Hair Cells, Auditory, Outer / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*; D017136:Ion Transport / Q000187:drug effects; D007700:Kinetics; D008027:Light; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008954:Models, Biological; D009578:Nitrobenzenes; D018408:Patch-Clamp Techniques; D010782:Photolysis; D011188:Potassium / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014269:Trifluoroacetic Acid / Q000494:pharmacology / Q000528:radiation effects
D002038:Bungarotoxins; D005463:Fluoroacetates; C088047:N-((alpha-carboxy)-2-nitrobenzyl)carbamoylcholine trifluoroacetate; D009578:Nitrobenzenes; D011978:Receptors, Nicotinic; D002217:Carbachol; D014269:Trifluoroacetic Acid; D011188:Potassium; D002118:Calcium