Induction of hypoxia inducible factor 1 by oxygen glucose deprivation is attenuated by hypoxic preconditioning in rat cultured neurons.
Hypoxia-inducible factor 1 (HIF-1) is a posttranscriptionally regulated transcription factor, controlling several hypoxia-inducible genes. Here we show a rapid and transient increase of HIF-1 DNA binding activity in a dose dependent manner in primary cortical neurons of rats exposed to oxygen glucose deprivation (OGD) for 30, 60, 90, or 120 min. A reduced induction of binding activity was observed when neurons were preconditioned by a non-lethal OGD interval (60 min) 48 h prior to the 90 min OGD. Thus, hypoxic preconditioning reduces activation of HIF-1 binding activity. The molecular mechanism of HIF-1 activation, its target genes, and its role in tolerance induction and/or ischemia induced damage remain to be elucidated.
Department of Neurology, Charité Hospital, Humboldt University, Berlin, Germany.
K
K
Ruscher
N
N
Isaev
G
G
Trendelenburg
M
M
Weih
L
L
Iurato
A
A
Meisel
U
U
Dirnagl
D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D003213:Conditioning, Psychological; D004247:DNA / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000172:deficiency*; D000860:Hypoxia / Q000378:metabolism / Q000503:physiopathology*; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009474:Neurons / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats / Q000196:embryology; D013997:Time Factors; D014157:Transcription Factors
D004268:DNA-Binding Proteins; C497444:Hif1a protein, rat; D051793:Hypoxia-Inducible Factor 1; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009687:Nuclear Proteins; D014157:Transcription Factors; D004247:DNA; D005947:Glucose