Regulation of parathyroid hormone-related protein expression in a canine squamous carcinoma cell line by colchicine.
The regulation of parathyroid hormone-related protein expression by colchicine, vinblastine, nocodazole, taxol, transforming growth factor-beta1 (TGFbeta1), and epidermal growth factor (EGF) was investigated in a canine squamous carcinoma cell line (SCC 2/88 cells). SCC 2/88 cells were stably transfected with a human P2/P3 PTHrP promoter-luciferase reporter gene construct and gene expression was measured after chemical treatments. The greatest increase in reporter gene expression was observed after colchicine treatment and small increases occurred after treatment with vinblastine, taxol, TGFbeta1, or EGF. Nocodazole had no significant effect on reporter gene expression. Colchicine also increased PTHrP steady state mRNA expression and PTHrP secretion by SCC 2/88 cells. These results demonstrated that PTHrP production was increased in SCC 2/88 cells by colchicine and suggested that factors or events during mitosis are capable of stimulating PTHrP production. An increase in PTHrP production during mitosis of malignant epithelial cells may be important in the pathogenesis of humoral hypercalcemia of malignancy.
Department of Veterinary Biosciences, The Ohio State University, Columbus, USA.
A
A
Gröne
M T
MT
Weckmann
C C
CC
Capen
T J
TJ
Rosol
D000818:Animals; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology*; D004285:Dogs; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects*; D008156:Luciferases / Q000378:metabolism; D008162:Lumicolchicines / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000235:genetics* / Q000378:metabolism; D015739:Nocodazole / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011863:Radioimmunoassay; D014158:Transcription, Genetic; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology
D008162:Lumicolchicines; D009363:Neoplasm Proteins; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C042109:lumicolchicine; D014747:Vinblastine; D004815:Epidermal Growth Factor; D008156:Luciferases; D017239:Paclitaxel; D015739:Nocodazole; D003078:Colchicine