Deletion of bone marrow stromal cell antigen-1 (CD157) gene impaired systemic thymus independent-2 antigen-induced IgG3 and mucosal TD antigen-elicited IgA responses.
Bone marrow stromal cell Ag-1 (BST-1; CD157)-deficient mice were generated to examine the immunologic roles of the molecule in vivo. In BST-1(-/-) mice, the development of peritoneal B-1 cells was delayed, and CD38(low/-) B-lineage cells were increased in the bone marrow and spleen. Partial impairment of thymus-independent (TI-2) and thymus-dependent (TD) Ag-specific immune responses was noted in the systemic and mucosal compartments of BST-1(-/-) mice, respectively. Although serum Ig levels as well as TD and TI-1 Ag-specific systemic immune responses were normal, the TI-2 Ag-induced IgG3 response was selectively impaired. Oral immunization of BST-1(-/-) mice with cholera toxin, a potent TD Ag for the induction of IgA response, resulted in the poor production of Ag-specific Abs at the intestinal mucosa accompanied by the reduced number of Ag-specific IgA-producing cells in the lamina propria. These results indicate that BST-1 has roles in B cell development and Ab production in vivo.
Division of Molecular Oncology, Biomedical Research Center, Osaka University Medical School, Suita, Japan.
M
M
Itoh
K
K
Ishihara
T
T
Hiroi
B O
BO
Lee
H
H
Maeda
H
H
Iijima
M
M
Yanagita
H
H
Kiyono
T
T
Hirano
D036541:ADP-ribosyl Cyclase; D000818:Animals; D015703:Antigens, CD; D000955:Antigens, T-Independent / Q000276:immunology*; D001854:Bone Marrow Cells / Q000276:immunology*; D058851:GPI-Linked Proteins; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000276:immunology; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D018345:Mice, Knockout; D017154:Stromal Cells / Q000276:immunology*
D015703:Antigens, CD; D000955:Antigens, T-Independent; D058851:GPI-Linked Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C087521:ADP-ribosyl cyclase 2