A YAC contig in Xq22.3-q23, from DXS287 to DXS8088, spanning the brain-specific genes doublecortin (DCX) and PAK3.
Although several genes for mental retardation and epilepsy, including double cortex/X-linked lissencephaly (DC/XLIS), have been localized to Xq21.3-q23, there has been no complete physical map of this region available. We constructed a YAC/STS contig map by initiating two yeast artificial chromosome (YAC) walks from the markers that flanked the DC/XLIS candidate gene region. We report an approximately 4-Mb contig extending from DXS287 to DXS8088, encompassing DXS1072 and DXS1059, and composed of 52 YACs identified with 15 previously published STSs and 19 novel YAC-end STSs. This contig also contains two brain-specific genes, doublecortin (HGMW-approved symbol DCX), responsible for DC/XLIS, and PAK3, which may be responsible for neurological diseases localized to this region. The new contig extends and incorporates several previously published contigs, providing a total overlapping contig extending approximately 34 Mb from DXS441 in Xq13.1 to DXS8088 in Xq23.
Department of Neurology, Harvard Medical School, Boston, Massachusetts, 02115, USA.
K M
KM
Allen
J G
JG
Gleeson
S M
SM
Shoup
C A
CA
Walsh
D001921:Brain / Q000378:metabolism*; D016386:Chromosome Walking; D020451:Contig Mapping; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005796:Genes / Q000235:genetics*; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D016324:Sequence Tagged Sites; D014960:X Chromosome / Q000235:genetics*; D054462:p21-Activated Kinases
C000714328:DCX protein, human; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; C515911:PAK3 protein, human; D017346:Protein Serine-Threonine Kinases; D054462:p21-Activated Kinases