Influence of cell surface glycoprotein gC produced by pseudorabies virus on cytopathic effect.
The wild-type pseudorabies virus (WT-PRV) produced a round-type cytopathic effect (CPE) in PK-15 cell line of porcine kidney origin, while PRVgCs lacking in gC-transmembrane-anchor region and PRVgC-defecting in gC gene produced a syncytium-type CPE. The mouse embryo cell line (BALB/3T3 clone A31) were transfected with recombinant plasmid of pcDNA3 which incorporated with gC gene. The transfected A31/gC cells were stably expressing gC. Only a round-type CPE was observed in these cells infected with WT-PRV, while a syncytium-type CPE was observed in the cells infected with each of the PRVgCs and PRVgC-. Any viruses described above induced a syncytium-type CPE in A31/pcDNA cells transfected with a plasmid without gC gene. By WT-PRV infection, PK-15 cells generated about 2- or 8-fold more gC than the A31/gC and A31/pcDNA cells when gC was measured by hemagglutination test. Flowcytometric analysis revealed that amount of gC on the cell surface of A31/gC and PK-15 cells increased after infection with WT-PRV. Round-type CPE was observed with the increase of gC. These results suggest that the type of CPE formation induced by PRV is dominated by the amount of gC on the infected cell surface.
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
S
S
Katayama
N
N
Okada
T
T
Ohgitani
T
T
Kokubu
Y
Y
Shimizu
D016475:3T3 Cells; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002522:Chlorocebus aethiops; D002999:Clone Cells; D015752:Genes, env; D015726:Giant Cells; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000502:physiology*; D007668:Kidney; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis; D013552:Swine; D014162:Transfection; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D011994:Recombinant Proteins; D014759:Viral Envelope Proteins