Inhibitory action of CGS 21680 on cerebral cortical neurons is antagonized by bicuculline and picrotoxin-is GABA involved?
The possibility of an involvement of endogenously released GABA in the inhibitory actions of A1 and A2a adenosine receptor agonists on rat cerebral cortical neurons discharges was examined using the GABAA antagonists bicuculline and picrotoxin. The A1 agonist N6-cyclopentyladenosine (CPA), the A2a agonist CGS 21680 and the non-selective receptor agonist, adenosine, depressed neuronal firing. Applications of bicuculline or picrotoxin enhanced the spontaneous firing rate of cortical neurons, indicating the presence of ongoing GABA-ergic inhibition. Antagonism of GABAA receptors blocked the depressant effects of CGS 21680 on neuronal firing; was without effect on CPA-evoked inhibitions and tended to reduce the duration of adenosine-evoked inhibitions. These results suggest that the depressant effects of A2a receptor activation are due to an increase in GABA-ergic inhibition, likely as a consequence of increased GABA release. GABA does not appear to be involved in adenosine A1 receptor-mediated inhibition of neuronal firing.
Department of Physiology, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201-1928, USA. jphillis@med.wayne.edu
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JW
Phillis
D000200:Action Potentials / Q000187:drug effects; D000241:Adenosine / Q000031:analogs & derivatives* / Q000037:antagonists & inhibitors / Q000494:pharmacology; D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects*; D018756:GABA Antagonists / Q000494:pharmacology*; D008297:Male; D009474:Neurons / Q000187:drug effects*; D010627:Phenethylamines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D010852:Picrotoxin / Q000494:pharmacology; D058906:Purinergic P1 Receptor Agonists; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
D000241:Adenosine; D001640:Bicuculline; D018756:GABA Antagonists; D010627:Phenethylamines; D010852:Picrotoxin; D058906:Purinergic P1 Receptor Agonists; D005680:gamma-Aminobutyric Acid; C061282:2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine; C048599:N(6)-cyclopentyladenosine