A novel method for concurrent visualization of immunostain under light and electron microscopy in pancreatic islets.
We developed a simple method employing the use of flat-embedding techniques on thick frozen sections which allows correlation of light and electron microscopic immunohistochemistry. This method has been particularly useful in visualization of pancreas sections, an adaptation especially important because this tissue is not amenable to conventional vibratome sectioning. In this study we demonstrate the use of this technique to examine the same tissue section at the light and the electron microscopic level while maintaining morphology.
Wong
F S
FS
Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Visintin
I
I
Shanabrough
M
M
Leranth
C
C
Janeway
C A
CA
D000818:Animals; D016827:CD8 Antigens / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism / Q000648:ultrastructure; D005629:Frozen Sections; D007150:Immunohistochemistry / Q000379:methods*; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism* / Q000648:ultrastructure*; D051379:Mice; D016688:Mice, Inbred NOD; D008854:Microscopy, Electron / Q000379:methods*
D016827:CD8 Antigens; D007328:Insulin