title: Expression of the intermediate filament keratin gene, K15, in the basal cell layers of epithelia and the hair follicle.
abstract: The intermediate filament keratin, K15, is present in variable abundance in stratified epithelia. In this study we have isolated and characterized the sheep K15 gene, focusing on its expression in the follicles of sheep and mice. We show that K15 is expressed throughout the hair cycle in the basal layer of the outer root sheath that envelops the follicle. Strikingly, however, in large medullated wool follicles, a small group of basal outer root sheath cells located in the region thought to contain hair follicle stem cells are K15-negative. In the follicle bulb K15 is expressed in cells situated next to the dermal papilla but not in the inner bulb cells. Elsewhere, K15 is expressed at a low, variable level in the basal layer of the epidermis and sebaceous gland, often in a punctate pattern. In the esophagus of the sheep K15 expression is restricted to the basal layer, in contrast to human esophagus where it is expressed throughout the epithelium. Transgenic mouse lines established with a 15-kb sheep K15 gene construct exhibited faithful expression and showed no phenotypic consequences of K15 overexpression. An investigation of transgene expression showed that K15 is continuously expressed in outer root sheath cells during the hair cycle. Given its expression in the mitotically active basal cell layers of diverse epithelia and the follicle, K15 expression appears to signal an early stage in the pathway of keratinocyte differentiation that precedes the decision of a cell to become epidermal or hair-like.
authors.affiliation: John Curtin School of Medical Research, Australian National University, Acton, ACT, 0200, Australia.
authors.forename: L A
authors.initials: LA
authors.lastname: Whitbread
authors.forename: B C
authors.initials: BC
authors.lastname: Powell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004817:Epidermis; D004847:Epithelial Cells / Q000378:metabolism*; D004947:Esophagus / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D053569:Keratin-15; D007633:Keratins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D012627:Sebaceous Glands / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep
chemical_list: D053569:Keratin-15; C492053:Krt15 protein, mouse; D007633:Keratins