title: Fluorescence and surface-enhanced Raman study of 9-aminoacridine in relation to its aggregation and excimer emission in aqueous solution and on silver surface.
abstract: Fluorescence spectroscopy and surface-enhanced Raman spectroscopy (SERS) have been applied to study the aggregation and excimer emission of 9-aminoacridine (9AA) and 9-aminoacridine hydrochloride (9AA-HCl) in aqueous solution and on silver colloids. The effect of the drug concentration, pH, and chloride concentration on these processes has been investigated. The excimer emission of 9AA is connected to the dimerization of this drug in solution: the formation of 9AA dimers is greatly favored when the drug is under the amino form at neutral and acidic pH, while at alkaline pH the imino 9AA form tends to form large-size aggregates which cannot be excited to render excimer emission. 9AA is adsorbed on the silver surface under two different forms: strongly and weakly attached 9AA, each one corresponding to the different drug tautomers: imino and amino. The interaction of 9AA with silver induces a charge transfer from the adsorbate to the metal leading to a remarkable fluorescence quenching, a basicity decrease of the adsorbed drug and a considerable weakening of the dimer-excimer emission. Furthermore, an attribution of the main Raman features appearing in the SERS spectra has been proposed, providing marker bands for the imino and amino 9AA tautomers, and a mechanism for the molecular dimerization is also suggested.
authors.affiliation: Dep. de Dermatología, Hospital Universitario San Carlos, Universidad Complutense de Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Murza
authors.forename: S
authors.initials: S
authors.lastname: Sánchez-Cortés
authors.forename: J V
authors.initials: JV
authors.lastname: García-Ramos
mesh_terms: D000585:Aminacrine / Q000032:analysis / Q000737:chemistry*; D003102:Colloids; D012834:Silver; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014867:Water
chemical_list: D003102:Colloids; D012996:Solutions; D014867:Water; D012834:Silver; D000585:Aminacrine