title: Enzyme-linked immunosorbent assay for detection of IgG against penicillins in children.
abstract: OBJECTIVE
  To establish an enzyme-linked immunosorbent assay (ELISA) for detection of IgG against penicillins and then investigate this antibody response in children.
  
  
  METHODS
  Western blotting, ELISA, and hapten inhibition assay were used.
  
  
  RESULTS
  Penicillins reacted with bovine serum albumin (BSA) to generate conjugate which was used as coating antigen (100 mg.L-1) in ELISA. A good correlation was found between absorbance (A) and dilutions of sample with a linear coefficient of 0.9918. Thirteen subjects who were clinically suspicious of penicillin allergy were tested for specific IgG by ELISA, 4 positive for benzylpenicillin, 1 for benzylpenicillin and piperacillin, and 3 for benzylpenicillin, piperacillin and ampicillin.
  
  
  CONCLUSIONS
  An ELISA was successfully established and penicillin reactive IgG response in children was heterogeneous. IgG antibodies recognized benzylpenicillin molecule, degraded products and new antigenic determinant from conjugate. Cross reactions occurred among benzylpenicillin, ampicillin and piperacillin.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Shanghai Medical University, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: Y M
authors.initials: YM
authors.lastname: Wang
authors.forename: G
authors.initials: G
authors.lastname: Shen
authors.forename: B Y
authors.initials: BY
authors.lastname: Chen
mesh_terms: D002648:Child; D002675:Child, Preschool; D004342:Drug Hypersensitivity / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007223:Infant; D010406:Penicillins / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D010406:Penicillins