Characteristics of Yersinia pseudotuberculosis isolated from animals in Brazil.
Strains (105) of Yersinia pseudotuberculosis isolated in Brazil between 1982 and 1990 were bio-serotyped. They were also studied for plasmid profile, autoagglutination and calcium dependence at 37 degrees C, Congo red uptake, pyrazinamidase activity, esculin hydrolysis, salicin fermentation and drug sensitivity: 95.24% were biotype 2, serogroup O:3; 2.86% were biotype 1, serogroup O:1; and 1.90% were biotype 2, non-agglutinable. Plasmids were found in 77.14% of the strains (one in each strain). There was total correlation between the presence of the virulence plasmid and autoagglutination, calcium dependence at 37 degrees C and Congo red uptake. The esculin, salicin and pyrazinamidase tests were not efficient in differentiating pathogenic from non-pathogenic Y. pseudotuberculosis isolates. All strains were highly sensitive to the drugs used. These results indicate that Y. pseudotuberculosis is a potential pathogen for humans in Brazil, especially because the bio-serogroups detected among animals are those most frequently associated with human diseases.
Martins
C H
CH
Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas-UNESP, Araraquara, São Paulo, Brazil.
Bauab
T M
TM
Falcão
D P
DP
D000581:Amidohydrolases / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D001592:Benzyl Alcohols / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003224:Congo Red / Q000378:metabolism; D004929:Esculin / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005960:Glucosides; D006801:Humans; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000032:analysis; D012703:Serotyping; D014774:Virulence; D015011:Yersinia pseudotuberculosis / Q000145:classification* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology / Q000662:veterinary*
D000900:Anti-Bacterial Agents; D001592:Benzyl Alcohols; D005960:Glucosides; D004929:Esculin; D003224:Congo Red; C005696:salicin; D000581:Amidohydrolases; C022490:pyrazinamide deamidase; D002118:Calcium