Dimerization of native myosin LC2(RLC)-free subfragment 1 from adult rabbit skeletal muscle.
We reinvestigated whether the native myosin LC2-free-subfragment 1 (S1) dimer exists by using viscometry, capillary electrophoresis, and laser light scattering. We found that the intrinsic viscosity of the monomer is [eta]m = 6.7 cm3/g and its translation diffusion coefficient is (c = 0) = 4.43 x 10(-)7 cm2/s. For the dimer, [eta]d = 19.8 cm3/g and (c = 0) = 2.54 x 10(-)7 cm2/s. Using the Svedberg equation and introducing the values of the sedimentation coefficients (5.05 S for the monomer and 6.05 S for the dimer), we find the following molecular weights: Mr,m = 108 000 Da and Mr,d = 213 000 Da, which agree well with previous determinations. Capillary electrophoresis successfully separated S1(A1) and S1(A2), in a monomer buffer, and S1(A1) and S1(A2) and a heterodimer S1(A1)-S1(A2), in a dimer buffer. An interesting feature of the monomer-dimer equilibrium is the presence of temperature transitions, whose positions and widths depend upon the buffer conditions. At low temperatures, a pure dimer was observed, whereas at high temperatures only the monomer was present. The dimerization site on both myosin and S1 is extremely labile.
Morel
J E
JE
Laboratoire de Biologie, Ecole Centrale de Paris, Châtenay-Malabry, France.
Taouil
K
K
D'hahan
N
N
Aguilar
A
A
Merah
Z
Z
Dalbiez
J P
JP
Bayol
P
P
Guillo
N
N
Patard
L
L
Cabane
V
V
Ferrari
M
M
Figuera Picazo
G
G
Dam Hieu
H
H
Francin
M
M
D000266:Adenylyl Imidodiphosphate / Q000378:metabolism; D000818:Animals; D002021:Buffers; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D019281:Dimerization; D019075:Electrophoresis, Capillary; D008956:Models, Chemical; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D018994:Myosin Light Chains / Q000737:chemistry / Q000378:metabolism*; D015879:Myosin Subfragments / Q000737:chemistry / Q000378:metabolism*; D011817:Rabbits; D014783:Viscosity
D002021:Buffers; D018994:Myosin Light Chains; D015879:Myosin Subfragments; D000266:Adenylyl Imidodiphosphate; D017301:Ca(2+) Mg(2+)-ATPase