Estrogen attenuates endothelin-1 production by bovine endothelial cells via estrogen receptor.
To investigate the mechanism underlying the antiatherogenic effect of estrogen, we examined the role of estrogen in the regulation of endothelin-1 (ET-1) production by cultured bovine carotid arterial endothelial cells. Treatment of the cells with 17beta-estradiol dose dependently inhibited gene expression and peptide secretion of ET-1. This inhibitory effect of 17beta-estradiol was more marked when ET-1 production was stimulated by transforming growth factor-beta1. Simultaneous addition of ICI 182,780, a pure estrogen receptor antagonist, completely blocked the effect of 17beta-estradiol, indicating that 17beta-estradiol acted via estrogen receptor. These results suggest that reduced ET-1 production by endothelial cells may be involved in the vasoprotective effect of estrogen.
Akishita
M
M
Department of Geriatric Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113-8655, Japan.
Kozaki
K
K
Eto
M
M
Yoshizumi
M
M
Ishikawa
M
M
Toba
K
K
Orimo
H
H
Ouchi
Y
Y
D000818:Animals; D002339:Carotid Arteries; D002417:Cattle; D002474:Cell-Free System; D002478:Cells, Cultured; D019332:Endothelin-1 / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D011960:Receptors, Estrogen / Q000502:physiology*
D019332:Endothelin-1; D012333:RNA, Messenger; D011960:Receptors, Estrogen; D004958:Estradiol