Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.).
The in vivo and in vitro pathogenic activities of whole cells and extracellular products of Vibrio alginolyticus for cultured gilt-head sea bream were evaluated. The 50% lethal doses ranged from 5.4 x 10(4) to 1.0 x 10(6) CFU/g of body weight. The strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. In addition, the in vitro ability of V. alginolyticus to adhere to mucus and skin cells of sea bream was demonstrated by scanning electron microscopy. The biological activities of extracellular products of V. alginolyticus were hydrolytic activities; the products were able to degrade sea bream mucus. V. alginolyticus was cytotoxic for fish cell lines and lethal for sea bream. Moreover, the extracellular products could degrade sea bream tissues. However, experiments performed with the bath immersion inoculation technique demonstrated that V. alginolyticus should be considered a pathogen for sea bream only when the mucus layer is removed and the skin is damaged.
Balebona
M C
MC
Departments of Microbiology, Faculty of Sciences, University of Malaga, Campus de Teatinos, 29071 Malaga, Spain.
Andreu
M J
MJ
Bordas
M A
MA
Zorrilla
I
I
Moriñigo
M A
MA
Borrego
J J
JJ
D000818:Animals; D001422:Bacterial Adhesion; D001427:Bacterial Toxins / Q000633:toxicity; D002474:Cell-Free System; D004912:Erythrocytes / Q000187:drug effects; D005393:Fish Diseases / Q000382:microbiology* / Q000473:pathology; D005880:Gills / Q000382:microbiology; D006386:Hemagglutination Tests; D006736:Horses; D007413:Intestinal Mucosa / Q000382:microbiology; D018482:Muscle, Skeletal / Q000382:microbiology / Q000473:pathology; D010473:Perciformes / Q000382:microbiology*; D012867:Skin / Q000382:microbiology / Q000473:pathology / Q000648:ultrastructure; D014733:Vibrio / Q000302:isolation & purification / Q000472:pathogenicity* / Q000502:physiology; D014735:Vibrio Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D014774:Virulence
D001427:Bacterial Toxins