title: Antigenic variation and cross-reactivity in Bacteroides forsythus clinical isolates detected by western blot.
abstract: Bacteroides forsythus is one of the etiologic agents of destructive periodontal diseases. Determining which antigenic components of the bacterium are recognized in the immune response of periodontitis patients is an important step in assessing strategies for vaccine development. The aim of this study was to identify the major strain-variable and cross-reactive antigens of B. forsythus clinical isolates recognized by serum IgG from patients with early-onset rapidly progressive periodontitis. Ten patient sera with measurable IgG against antigenic components of the species were identified by Western blot. Positive sera were tested by checkerboard ELISA to identify those most responsive to strain-variable antigens in nine clinical isolates and ATCC strain 43037. Correlation analysis of the ELISA data suggested that different subsets of isolates were preferentially recognized by different sera. Western blots revealed that certain sera also recognized major shared components across all the isolates, but preferential recognition of different isolate subsets by different patients was clearly confirmed. To determine if the variable antigens recognized were nonprotein, proteinase K-digested isolates were compared to undigested controls by Western blot. The main strain-variable antigens were proteinase resistant, while proteins at 200 and 210 kDa were identified as the major shared components. Two-dimensional SDS-PAGE revealed that these proteins are the quantitatively dominant heat-modifiable components of the cell envelope. Even though variable antigens are prominent in the immune response of patients, a cross-protective vaccine based on the shared envelope proteins of B. forsythus seems feasible in light of these observations.
authors.affiliation: Research Center in Oral Biology, University of Washington, Seattle 98195, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Sims
authors.forename: L A
authors.initials: LA
authors.lastname: Mancl
authors.forename: P H
authors.initials: PH
authors.lastname: Braham
authors.forename: B W
authors.initials: BW
authors.lastname: Bainbridge
authors.forename: R C
authors.initials: RC
authors.lastname: Page
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000276:immunology; D000940:Antigenic Variation / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology*; D001439:Bacteroides / Q000276:immunology* / Q000302:isolation & purification; D001442:Bacteroides Infections / Q000276:immunology / Q000382:microbiology; D015153:Blotting, Western; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008970:Molecular Weight; D010518:Periodontitis / Q000276:immunology / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G