Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers.
A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries.
Chansiri
K
K
Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand. kosum@psm.swu.ac.th
Kawazu
S
S
Kamio
T
T
Fujisaki
K
K
Panchadcharam
C
C
Watanapokasin
Y
Y
Uthaisang
W
W
Tananyutthawongese
C
C
Sarataphan
N
N
D000818:Animals; D001210:Asia, Southeastern / Q000453:epidemiology; D001315:Australia / Q000453:epidemiology; D002417:Cattle; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D018503:Genome, Protozoan; D007564:Japan / Q000453:epidemiology; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary; D015203:Reproducibility of Results; D013045:Species Specificity; D016794:Theileria / Q000145:classification* / Q000235:genetics; D013801:Theileriasis / Q000453:epidemiology / Q000469:parasitology*
D017931:DNA Primers; D016054:DNA, Protozoan