pBLA8, from Brevibacterium linens, belongs to a gram-positive subfamily of ColE2-related plasmids.
A 3.1 kb DNA fragment from pBLA8, a Brevibacterium linens cryptic plasmid, containing all the information required for autonomous replication was cloned and sequenced. Using deletion analysis, the fragment essential and sufficient for autonomous replication was delimited to 1.5 kb. This fragment is characterized by the presence of an ori site located upstream of an operon encoding two proteins, RepA and RepB, both essential for replication. Based on structural similarities and a strong conservation of ori, RepA and RepB, pBLA8 was assigned to a new subfamily of the ColE2 plasmid family. This subfamily is distinguished by the requirement for two Rep proteins and the location of an ori site upstream of the repAB operon. RepA is thought to encode primase activity, whereas RepB could be a DNA-binding protein. An Escherichia coli-B. linens shuttle vector, derived from pBLA8, was constructed. Its host spectrum was extended to Arthrobacter species.
Leret
Veronique
V
Trautwetter
Annie
A
Rind
Alain
A
Blanco
Carlos
C
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D003086:Bacteriocin Plasmids / Q000235:genetics; D001483:Base Sequence; D001951:Brevibacterium / Q000235:genetics*; D004265:DNA Helicases; D019915:DNA Primase / Q000235:genetics; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006094:Gram-Positive Bacteria / Q000235:genetics; D019077:Helix-Turn-Helix Motifs / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D017433:Protein Structure, Secondary; D011506:Proteins / Q000235:genetics; D012093:Replicon / Q000235:genetics*; D015183:Restriction Mapping; D016415:Sequence Alignment; D017384:Sequence Deletion / Q000235:genetics; D015534:Trans-Activators
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D011506:Proteins; C048817:RepB protein, Bacteria; D015534:Trans-Activators; C045264:replication initiator protein; D019915:DNA Primase; C023140:DNA replicase; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases