title: Mass spectrometric survey of interganglionically transported peptides in Aplysia.
abstract: The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.
authors.affiliation: Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: T P
authors.initials: TP
authors.lastname: Moroz
authors.forename: R W
authors.initials: RW
authors.lastname: Garden
authors.forename: P D
authors.initials: PD
authors.lastname: Floyd
authors.forename: K R
authors.initials: KR
authors.lastname: Weiss
authors.forename: J V
authors.initials: JV
authors.lastname: Sweedler
mesh_terms: D000818:Animals; D001048:Aplysia / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D001693:Biological Transport, Active / Q000502:physiology; D002490:Central Nervous System / Q000737:chemistry; D017952:Ganglia, Invertebrate / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D009479:Neuropeptides / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: C094043:LUQIN; D009479:Neuropeptides; C103535:cerebral peptide 1