title: High level expression and structural characterisation of herpes simplex virus type I transcriptional activator VP16 (alpha-trans inducing factor).
abstract: We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % alpha-helix and 17.5 % beta-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies.
authors.affiliation: Institut für Bakteriologie, Mykologie, und Hygiene, Veterinärmedizinische Universität Wien, Veterinaerplatz 1, Vienna, A-1210, Austria. Martin.Spatz@vu-wein.ac.at
authors.forename: A M
authors.initials: AM
authors.lastname: Spatz
authors.forename: S A
authors.initials: SA
authors.lastname: Ali
authors.forename: M
authors.initials: M
authors.lastname: Auer
authors.forename: C
authors.initials: C
authors.lastname: Graf
authors.forename: M M
authors.initials: MM
authors.lastname: Eibl
authors.forename: A
authors.initials: A
authors.lastname: Steinkasserer
mesh_terms: D016367:Baculoviridae / Q000235:genetics; D002942:Circular Dichroism; D015967:Gene Expression Regulation, Viral; D017405:Herpes Simplex Virus Protein Vmw65 / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000737:chemistry*; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
chemical_list: D017405:Herpes Simplex Virus Protein Vmw65; D011994:Recombinant Proteins