title: Comparison of the BAX for screening/E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef.
abstract: Escherichia coli O157:H7 is an important food-borne pathogen. Often E. coli O157:H7 is difficult to detect, because it is present sporadically at very low levels together with very high levels of competitor organisms which can be difficult to distinguish phenotypically. Cultural methods are time-consuming and give variable results in the detection of E. coli O157:H7. This study examined the performance of BAX for Screening/E. coli O157:H7, a new rapid method for the detection of E. coli O157:H7, against traditional and improved cultural methods and an immunodiffusion assay. All cultural methods demonstrated inadequacy in detecting the presence of E. coli O157:H7 in inoculated samples. The limitations of these cultural methods further complicate evaluation of screening methodologies. The BAX for Screening/E. coli O157:H7 assay outperformed the other methods, with a detection rate of 96.5%, compared to 39% for the best cultural method and 71.5% for the immunodiffusion method. The BAX for Screening/E. coli O157:H7 assay proved to be a rapid, highly sensitive test for the detection of low levels of E. coli O157:H7 in ground beef.
authors.affiliation: Deibel Laboratories, Madison, Wisconsin 53704, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Johnson
authors.forename: C L
authors.initials: CL
authors.lastname: Brooke
authors.forename: S J
authors.initials: SJ
authors.lastname: Fritschel
mesh_terms: D000818:Animals; D002417:Cattle; D004927:Escherichia coli Infections / Q000517:prevention & control / Q000635:transmission; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005511:Food Handling; D006801:Humans; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion / Q000379:methods; D008460:Meat / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors