title: cDNA cloning and predicted primary structure of scallop sarcoplasmic reticulum Ca(2+)-ATPase.
abstract: Sarcoplasmic reticulum (SR) Ca(2+)-ATPase of the scallop cross-striated adductor muscle was purified with deoxycholate and digested with lysyl endopeptidase for sequencing of the digested fragments. Overlapping cDNA clones of the ATPase were isolated by screening the cDNA library with an RT-PCR product as a hybridization probe, which encodes the partial amino acid sequence of the ATPase. The predicted amino acid sequence of the ATPase contained all the partial sequences determined with the proteolytic fragments and consisted of the 993 residues with approximately 70% overall sequence similarity to those of the SR ATPases from rabbit fast-twitch and slow-twitch muscles. An outline of the structure of the scallop ATPase molecule is predicted to mainly consist of ten transmembrane and five 'stalk' domains with two large cytoplasmic regions as observed with the rabbit ATPase molecules. The sequence relationship between scallop and other sarco/endoplasmic reticulum-type Ca(2+)-ATPases is discussed.
authors.affiliation: Biological Institute, Graduate School of Science, Osaka University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Nagata
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: M
authors.initials: M
authors.lastname: Ema
authors.forename: J
authors.initials: J
authors.lastname: Mimura
authors.forename: Y
authors.initials: Y
authors.lastname: Fujii-Kuriyama
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Furukohri
authors.forename: K
authors.initials: K
authors.lastname: Konishi
authors.forename: D
authors.initials: D
authors.lastname: Sato
authors.forename: G
authors.initials: G
authors.lastname: Tajima
authors.forename: J
authors.initials: J
authors.lastname: Nakamura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000737:chemistry*; D003001:Cloning, Molecular; D008565:Membrane Proteins / Q000737:chemistry; D008969:Molecular Sequence Data; D008974:Mollusca / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D010802:Phylogeny; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D017422:Sequence Analysis, DNA
chemical_list: D008565:Membrane Proteins; D000252:Calcium-Transporting ATPases