[The standardization of an ultramicro ELISA assay for the detection of IgG antibodies to the respiratory syncytial virus].
An ultramicro ELISA assay of double antibody for the detection of IgG antibodies to the respiratory syncytial virus (RSV) was standardized. It was used a RVS antiprotein F monoclonal antibody produced by the Genetic Engineering and Biotechnology Center (GEBC) in Havana. The use of this antibody allowed to include crude antigenic preparations instead of purified fractions, which caused a significant reduction of the reactivity obtained with the antigen control. The assay conditions were determined by crossed titration. It was obtained a sensitivity of 97.2%, a coincidence of 91%, and a specificity of 83.3% of the UMELISA as regards the complement fixation. The results may be qualitatively expressed or by antibody titres using only one serum dilution (1:40) and a pattern curve.
Savón
C
C
Instituto de Medicina Tropical Pedro Kourí.
Laferté
J
J
Goyenechea
A
A
Valdivia
A
A
Morier
L
L
Tejeiro
Y
Y
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005069:Evaluation Studies as Topic; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D018113:Respiratory Syncytial Virus, Human / Q000276:immunology*; D012680:Sensitivity and Specificity; D014760:Viral Fusion Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D014760:Viral Fusion Proteins