Isolation of the factor VIII-von Willebrand factor complex directly from plasma by gel filtration.
A high capacity gel filtration system was developed with the purpose of isolating factor VIII (FVIII) and von Willebrand factor (vWF) directly from plasma in significantly higher yields than obtained by cryoprecipitation, the technique most commonly used to recover FVIII-vWF from human plasma. After laboratory-scale gel filtration of plasma, a FVIII-containing fraction was collected containing about 90% of FVIII in the applied plasma and with almost tenfold higher purity than that obtained by cryoprecipitation. The gel filtration step has been scaled up for use as the initial step in the manufacturing process for a FVIII preparation (Nordiate).
Kaersgaard
P
P
HemaSure Denmark A/S, Gentofte.
Barington
K A
KA
D002850:Chromatography, Gel; D004563:Electrochemistry; D005169:Factor VIII / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007123:Immunoelectrophoresis, Two-Dimensional; D013696:Temperature; D014841:von Willebrand Factor / Q000302:isolation & purification* / Q000378:metabolism
D014841:von Willebrand Factor; D005169:Factor VIII