title: Characterization of erythromycin-resistant isolates of Staphylococcus aureus recovered in the United States from 1958 through 1969.
abstract: We tested 16 erythromycin-resistant clinical isolates of S. aureus, recovered from patients hospitalized in the United States from 1958 to 1969, for the presence of ermA, ermB, and ermC by using PCR. Fifteen of 16 isolates contained at least one copy of ermA; the remaining isolate, which was also clindamycin resistant, contained ermB. Eight of the 15 isolates harboring ermA, all of which were inducible, contained a single copy of the gene in the chromosome, while the remaining seven isolates had two copies of the gene. ermB was plasmid encoded and mediated constitutive resistance to erythromycin.
authors.affiliation: Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
authors.forename: F G
authors.initials: FG
authors.lastname: Nicola
authors.forename: L K
authors.initials: LK
authors.lastname: McDougal
authors.forename: J W
authors.initials: JW
authors.lastname: Biddle
authors.forename: F C
authors.initials: FC
authors.lastname: Tenover
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis; D004352:Drug Resistance, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field; D004917:Erythromycin / Q000494:pharmacology*; D006801:Humans; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D013997:Time Factors; D014481:United States
chemical_list: D004269:DNA, Bacterial; D004917:Erythromycin