Kinetics of ultraweak light emission from human erythroleukemia K562 cells upon electroporation.
Electroporation involves the application of an electric pulse that creates transient aqueous channels (electropores) across the lipid bilayer membranes. Here, we describe an instrument set up suitable to record ultraweak light emission from human erythroleukemia K562 cells during and immediately after delivery of electric pulses. Most of light was emitted in the first seconds after each pulse, following a complex decay which can be fitted by a double exponential equation characterized by two different time constants (T1 and T2), both in the order of seconds. T1 was approximately 10-fold shorter than T2 and both time constants were dependent on field strength of the electric pulse. The effect of various antioxidants on the amount of emitted photons and on T1 and T2 values was investigated, in order to shed some light on the chemical species responsible for cellular luminescence.
Maccarrone
M
M
Department of Experimental Medicine and Biochemical Sciences, University of Rome 'Tor Vergata', Via di Tor Vergata 135, I-00133 Rome, Italy.
Fantini
C
C
Agrò
A F
AF
Rosato
N
N
D002460:Cell Line; D004574:Electromagnetic Fields; D018274:Electroporation; D006801:Humans; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D008027:Light; D008054:Lipid Peroxides / Q000737:chemistry; D008163:Luminescent Measurements; D017382:Reactive Oxygen Species
D008054:Lipid Peroxides; D017382:Reactive Oxygen Species