Attachment and metabolic activity of hepatocytes cultivated on selected polymeric membranes.
Plasma or hormones added to hepatocyte incubation media mask the function of membranes as substrata per se for hepatocyte adhesion. This hypothesis was verified with hepatocyte cultures on various membranes in serum and hormone free medium. Freshly isolated rat hepatocytes were seeded on flat sheet membranes made of Cellulose Acetate (CA), aminated Cellulose Acetate (aCA), polysulfone (PSf) and sulfonated polysulfone (sPSf) and incubated in Hank's Balanced Salts Solutions (HBSS) as well as in William's E medium supplemented with newborn calf serum. It was found that PSf promoted hepatocyte adhesion most effectively. Good properties of PSf as a biomaterial for hepatocyte culture were confirmed in both media cultures. Urea synthesis and ammonia utilization measured in hepatocytes cultured on PSf were higher compared with other membrane cultures. PSf secured longer viability for a higher number of cells seeded on membrane compared with other investigated membranes, which is the reason for higher metabolic activity in PSf culture.
Jóźwiak
A
A
Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland.
Karlik
W
W
Wiechetek
M
M
Weryński
A
A
D000641:Ammonia / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials; D002448:Cell Adhesion / Q000502:physiology*; D002452:Cell Count; D002470:Cell Survival; D002482:Cellulose / Q000031:analogs & derivatives*; D003470:Culture Media; D008099:Liver / Q000166:cytology*; D008297:Male; D008567:Membranes, Artificial; D011108:Polymers; D051381:Rats; D017208:Rats, Wistar; D013450:Sulfones; D013499:Surface Properties; D014508:Urea / Q000378:metabolism
D001672:Biocompatible Materials; D003470:Culture Media; D008567:Membranes, Artificial; D011108:Polymers; D013450:Sulfones; C017662:polysulfone P 1700; C005062:acetylcellulose; D000641:Ammonia; D014508:Urea; D002482:Cellulose