A perfusion reversed-phase chromatographic method for ultrarapid determination of soybean proteins in soybean infant formulas and soybean milks: method development and validation.
Perfusion reversed-phase high-performance liquid chromatography (perfusion RP-HPLC) is applied for the first time to the determination of soybean proteins in commercial samples prepared from soybean protein isolate. A linear binary gradient of acetonitrile-water-0.1% trifluoroacetic acid at a flow rate of 3 mL/min and a temperature of 60 degrees C is optimized to analyze soybean proteins in approximately 3 min. Direct injection of samples into the chromatographic system is possible by dissolving them in water. Quantitation of soybean proteins is achieved by the proposed method using a soybean protein isolate as an external standard. The method is validated and applied to the quantitation of soybean proteins in infant formulas and powdered and liquid soybean milks.
García
M C
MC
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Madrid, Spain.
Torre
M
M
Marina
M L
ML
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D007231:Infant, Newborn; D030262:Soybean Proteins / Q000032:analysis*
D007202:Indicators and Reagents; D030262:Soybean Proteins