title: Molecular cloning of human ABPL, an actin-binding protein homologue.
abstract: Based on two partial cDNA sequences, a full-length cDNA sequence for an actin-binding like protein previously named ABPL has been isolated and characterized. ABPL is homologous to the human actin-binding proteins ABP-280 and ABP-278. The predicted sequence for ABPL is 2,705 amino acids in length with a calculated molecular mass of 289 kDa. It contains an amino terminal actin-binding domain followed by 24 tandem repeats of approximately 96 amino acids. Two hinge regions, Hinge I and Hinge II, were located prior to repeats 16 and 24, respectively. An isoform of ABPL lacking Hinge I, with a calculated molecular mass of 286 kDa, was also identified by the reverse transcriptase PCR (RT-PCR) method. A comparison with genomic sequences indicated the isoform resulted from alternative RNA splicing. ABPL has a unique insertion sequence of 82 amino acids in repeat 20 that was not present in the other two homologues and has a tissue distribution that was also different from the other two homologues.
authors.affiliation: Department of Biochemistry, University of Washington, Seattle, Washington, 98195, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Xie
authors.forename: W
authors.initials: W
authors.lastname: Xu
authors.forename: E W
authors.initials: EW
authors.lastname: Davie
authors.forename: D W
authors.initials: DW
authors.lastname: Chung
mesh_terms: D000199:Actins / Q000378:metabolism; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003285:Contractile Proteins / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D064448:Filamins; D006801:Humans; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011485:Protein Binding; D020033:Protein Isoforms / Q000235:genetics; D017386:Sequence Homology, Amino Acid
chemical_list: D000199:Actins; D003285:Contractile Proteins; D018076:DNA, Complementary; C578662:FLNB protein, human; D064448:Filamins; D008840:Microfilament Proteins; D020033:Protein Isoforms