Changes in outer membrane protein profiles of bacteria after meropenem-induced postantibiotic effect studied by capillary electrophoresis.
Persistent inhibition of bacterial growth, called postantibiotic effect (PAE), after a short exposure to a new carbapenem, meropenem, was determined in different strains of the Enterobacteriaceae family. Capillary electrophoresis (CE), as well as sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the outer membrane protein (OMP) profiles before and after meropenem treatment. CE proved to be suitable for the characterization of the OMP profiles of bacteria. Significant changes in the electrophoretic patterns were observed, showing the consequential effect of meropenem on bacteria.
Kustos
I
I
Department of Medical Microbiology and Immunology, Medical University of Pécs, Hungary. ikustos@apacs.pote.hu
Kocsis
B
B
Kerepesi
I
I
Kilár
F
F
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D000077731:Meropenem; D008970:Molecular Weight; D011511:Proteus / Q000187:drug effects / Q000378:metabolism; D012475:Salmonella / Q000187:drug effects / Q000378:metabolism; D012764:Shigella sonnei / Q000187:drug effects / Q000378:metabolism; D013845:Thienamycins / Q000494:pharmacology*
D001425:Bacterial Outer Membrane Proteins; D013845:Thienamycins; D000077731:Meropenem