Increased reactivity to 5-hydroxytryptamine of portal veins from mice infected with Schistosoma mansoni.
In chronic severe infection with Schistosoma mansoni, portal hypertension accompanied by anatomical changes of the portal vasculature can develop as a consequence of granulomatous response to eggs. Mice infected unisexually with male worms were used in the present study in order to investigate a direct effect of worms on the reactivity of their host portal vein. A higher reactivity in the presence of 5-hydroxytryptamine (5-HT), but not in the presence of KCl 100 mM solution, was observed in portal vein from infected mice compared to healthy mice. It was characterized by an increase in the maximal contraction and sensitivity to 5-HT. Blockade of NO-synthase with N omega-nitro-L-arginine methyl ester (L-NAME) induced a small increase in 5-HT potency in the portal vein from non-infected mice, but did not change the amplitude of the contractions. In portal veins from infected mice, preincubation with L-NAME did not affect the reactivity to 5-HT. Histological analysis indicated endothelial damage, subendothelial fibrous plaques, and focal areas of inflammatory infiltrates in the adventitial layer. As a conclusion, these results show that unisexual infection of mice with male S. mansoni increased the reactivity of the portal vein to 5-HT which seems to be only partially related to an alteration in the endothelial production of nitric oxide.
Silva
C L
CL
Departamento de Farmacologia Básica e Clínica, ICB, Universidade Federal do Rio de Janeiro, Ilha do Fundão, RJ, Brazil.
Morel
N
N
Lenzi
H L
HL
Noël
F
F
D000818:Animals; D000831:Animals, Newborn; D004730:Endothelium, Vascular / Q000503:physiopathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015726:Giant Cells; D008297:Male; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D011169:Portal Vein / Q000187:drug effects*; D011189:Potassium Chloride / Q000494:pharmacology; D012555:Schistosomiasis mansoni / Q000503:physiopathology*; D012701:Serotonin / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects*; D014662:Vasoconstrictor Agents / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D014662:Vasoconstrictor Agents; D012701:Serotonin; D011189:Potassium Chloride; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester