Detection of staphylococcal enterotoxin B and toxic shock syndrome toxin: PCR versus conventional methods.
An equal accuracy of commercially available kits, i.e., the reverse passive latex agglutination test (RPLA-Oxoid) and the Enzyme-Linked Immunosorbent Assay (SET-EIA Riedel Haen, Germany) to identify the presence of the staphylococcal enterotoxin B and the toxic shock syndrome toxin TSST-1 as well as the PCR-technique to detect the presence of the seb and tst-genes was demonstrated in 18 Staphylococcus aureus isolates. Selection of a primer pair from the etb gene sequence allowing simultaneously detection of the etb and tst-genes in S. aureus using a rapid DNA extraction method is emphasized.
Schumacher-Perdreau
F
F
Institut für Medizinische Mikrobiologie und Hygiene, Universität zu Köln, Germany.
Akatova
A
A
Pulverer
G
G
D001427:Bacterial Toxins; D004768:Enterotoxins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D007118:Immunoassay; D007841:Latex Fixation Tests / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods; D011933:Reagent Kits, Diagnostic; D013211:Staphylococcus aureus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D018089:Superantigens
D001427:Bacterial Toxins; D004768:Enterotoxins; D011933:Reagent Kits, Diagnostic; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal; C031818:enterotoxin B, staphylococcal