Cross-clade cytotoxic T cell response to human immunodeficiency virus type 1 proteins among HLA disparate North Americans and Thais.
A globally effective vaccine will need to elicit cytotoxic T lymphocytes (CTL) capable of recognizing diverse human immunodeficiency virus type 1 (HIV-1) clades. Study of the cellular immune responses of HIV-1-infected persons may allow predictions to be made regarding useful vaccine antigen components. The frequency and magnitude of CTL responses to clade E and B Gag, Pol-RT, Env, and Nef proteins were compared in 12 HLA-characterized, clade E-infected Thais and in 10 clade B-infected North Americans using vaccinia recombinant constructs for protein expression. While responses were detected against all proteins, they were most frequent and cross-reactive to Gag in both groups. Pol-RT was recognized less frequently in Thais than North Americans. Cross-clade protein recognition was common but not uniformly present among these HLA-disparate individuals. Population-specific CTL data are needed to adequately prepare for vaccine trials outside of North America and Europe.
Lynch
J A
JA
Department of Pediatrics, Walter Reed Army Medical Center, Washington DC, USA.
deSouza
M
M
Robb
M D
MD
Markowitz
L
L
Nitayaphan
S
S
Sapan
C V
CV
Mann
D L
DL
Birx
D L
DL
Cox
J H
JH
D044466:Asian People / Q000235:genetics; D044383:Black People / Q000235:genetics; D003429:Cross Reactions; D005431:Florida; D005787:Gene Frequency; D015488:HIV Antigens / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000145:classification / Q000276:immunology*; D015497:HIV-1 / Q000145:classification / Q000276:immunology*; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D044469:Racial Groups / Q000235:genetics*; D011994:Recombinant Proteins / Q000276:immunology; D012703:Serotyping; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D013785:Thailand; D014616:Vaccinia virus / Q000235:genetics; D044465:White People / Q000235:genetics; D001741:Black or African American
D015488:HIV Antigens; D006680:HLA Antigens; D011994:Recombinant Proteins