(+)-rhododendrol and epi-rhododendrin suppress the NO production by activated macrophages in vivo.
In this study, we investigated the effect of (+)-rhododendrol (1) and epi-rhododendrin (2) isolated from Acer nikoense Maxim. (Aceraceae) on nitric oxide (NO) production in mouse peritoneal macrophages elicited by bacillus Calmette-Guérin and in vitro stimulated by lipopolysaccharide. The NO production was not affected by an oral administration of methanol extract at a dose of 100 mg/kg/day. However, the AcOEt soluble fraction significantly reduced the NO production. (+)-Rhododendrol (1) isolated as an active substance from the AcOEt fraction suppressed the NO production. epi-Rhododendrin (2), the glucoside of (+)-rhododendrol (1) isolated from the n-BuOH fraction, also suppressed the NO production. As NO is one of the critical mediators in inflammation, these results suggest that (+)-rhododendrol (1) and epi-rhododendrin (2) contribute in part to the anti-inflammatory effect of A. nikoense.
Fushiya
S
S
Faculty of Pharmaceutical Sciences, Tohoku University, Sendai, Japan.
Kabe
Y
Y
Ikegaya
Y
Y
Takano
F
F
D000818:Animals; D000440:Butanols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010946:Plants, Medicinal / Q000737:chemistry; D013237:Stereoisomerism
D000440:Butanols; C115945:rhododendrol; D009569:Nitric Oxide