The heat-shock element is a functional component of the Arabidopsis APX1 gene promoter.
Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of the apx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1 promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shock cis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.
Storozhenko
S
S
Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Belgium.
De Pauw
P
P
Van Montagu
M
M
Inzé
D
D
Kushnir
S
S
D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D060387:Ascorbate Peroxidases; D001483:Base Sequence; D018983:DNA Footprinting; D017931:DNA Primers; D018076:DNA, Complementary; D006360:Heat-Shock Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D010544:Peroxidases / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D018076:DNA, Complementary; D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D010544:Peroxidases; D060387:Ascorbate Peroxidases