title: Activation by P2X7 agonists of two phospholipases A2 (PLA2) in ductal cells of rat submandibular gland. Coupling of the calcium-independent PLA2 with kallikrein secretion.
abstract: Isolated ductal cells of rat submandibular gland phospholipid pools were labeled with [3H]arachidonic acid (AA). The tracer was incorporated preferentially to phosphatidylcholine (46% of the lipidic fraction). Extracellular ATP induced the release of [3H]AA to the extracellular medium in a time- and dose-dependent manner (EC50 = 220 microM). Among other agents tested, only 2', 3'-O-(4-benzoylbenzoyl)adenosine 5'-triphosphate (Bz-ATP) was able to mimic the effect of ATP (EC50 = 15 microM), without activation of phospholipase C. The purinergic antagonists oxidized ATP, suramin, and Coomassie Blue partly inhibited the response to 1 mM ATP and 100 microM Bz-ATP; the response was also blocked by the addition of Mg2+ or Ni2+. Expression of P2X7 receptor mRNA in these cells was confirmed by reverse transcription-polymerase chain reaction. In the presence of extracellular calcium, the phospholipase A2 inhibitor 2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid (a nonspecific inhibitor), arachidonyl trifluoromethylketone (AACOCF3, an inhibitor of the calcium-dependent cytosolic PLA2 (cPLA2)), and bromoenol lactone (an inhibitor of the calcium-independent PLA2 (iPLA2)) inhibited the release of [3H]AA induced by ATP and Bz-ATP. In the absence of extracellular calcium, the release of [3H]AA in response to the purinergic agonists was still observed; this response was not affected by AACOCF3 and completely blocked by bromoenol lactone. ATP and Bz-ATP stimulated a calcium-independent secretion of kallikrein, which could be blocked by BEL but which was enhanced by AACOCF3. It is concluded that the P2X7 receptor in ductal cells is coupled to kallikrein secretion through a calcium-dependent cPLA2 and a calcium-independent iPLA2.
authors.affiliation: Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of the Basque Country, 48940 Leioa, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Alzola
authors.forename: A
authors.initials: A
authors.lastname: Pérez-Etxebarria
authors.forename: E
authors.initials: E
authors.lastname: Kabré
authors.forename: D J
authors.initials: DJ
authors.lastname: Fogarty
authors.forename: M
authors.initials: M
authors.lastname: Métioui
authors.forename: N
authors.initials: N
authors.lastname: Chaïb
authors.forename: J M
authors.initials: JM
authors.lastname: Macarulla
authors.forename: C
authors.initials: C
authors.lastname: Matute
authors.forename: J P
authors.initials: JP
authors.lastname: Dehaye
authors.forename: A
authors.initials: A
authors.lastname: Marino
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D016718:Arachidonic Acid / Q000378:metabolism; D002413:Cations, Divalent / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D007610:Kallikreins / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D008297:Male; D009532:Nickel / Q000378:metabolism; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D058910:Purinergic P2 Receptor Agonists; D011687:Purines / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018048:Receptors, Purinergic P2 / Q000235:genetics; D058486:Receptors, Purinergic P2X7; D013363:Submandibular Gland / Q000201:enzymology* / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; C546470:P2rx7 protein, rat; D058910:Purinergic P2 Receptor Agonists; D011687:Purines; D012333:RNA, Messenger; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D016718:Arachidonic Acid; D009532:Nickel; D000255:Adenosine Triphosphate; D010741:Phospholipases A; D054467:Phospholipases A2; D010738:Type C Phospholipases; D007610:Kallikreins; D008274:Magnesium