A plasmid that encodes three genes for resistance to macrolide antibiotics in Staphylococcus aureus.
In previous letters, FEMS Microbiol. Lett. 148 (1997) 91-96, it was demonstrated that plasmid pMS97-obtained from a in 1971 clinically isolated Staphylococcus aureus strain MS8968 resistant to macrolide (Mac) antibiotics--carried an msrA gene and uncharacterized erm gene, respectively. msrA encodes a cytoplasmic membrane protein that mediates the so-called 'active Mac-efflux' (designated hereafter as msrSA') and erm encodes a methyltransferase by which a specific adenine residue of 23S rRNA is modified: methylation prevents Mac antibiotics from binding to the 50S ribosomal subunit. Interestingly, we found, in addition, an mph-like gene (hereafter referred to as mphBM) present together with msrSA' and erm on pMS97. By a BLASTP analysis, the gene mphBM product has 49% identity and 67% similarity to the amino acid sequence of MPH(2')II encoded by mphB from Escherichia coli. The order of genes was 5'-msrSA'-mphBM-3', with a 342-base-pair spacer sequence. Although we have not yet determined where erm gene is located on pMS97, the gene seems to be downstream from mphBM. This finding suggests a warning to us concerning the imprudent use of antibiotics.
Matsuoka
M
M
Division of Microbiology, Hokkaido College of Pharmacy, Japan.
Endou
K
K
Kobayashi
H
H
Inoue
M
M
Nakajima
Y
Y
D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D018942:Macrolides; D026901:Membrane Transport Proteins; D008780:Methyltransferases / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; D026901:Membrane Transport Proteins; C115841:MphBM protein, Staphylococcus aureus; C066202:msrA protein, Staphylococcus epidermidis; D008780:Methyltransferases