Identification of quinine metabolites in urine after oral dosing in humans.
A gas chromatographic-mass spectrometric method was used to separate quinine and its metabolites present in urine after oral dosing of 300 mg quinine in humans. The technique allowed the separation of quinine and ten metabolites. Four of these metabolites were definitely identified as 3-hydroxyquinine, 2'-quinone, O-desmethylquinine and 10,11-dihydroxydihydroquinine, by comparing their methane chemical ionization mass spectra with those of authentic standards prepared by organic synthesis. Six other metabolites are described for the first time in human urine. From their electron impact and chemical ionization mass spectra, we propose these compounds to be 3-hydroxy-2'-quinone, O-desmethyl-2'-quinone, O-desmethyl-3-hydroxyquinine, O-desmethyl-3-hydroxy-2'-quinone, 10,11-dihydroxydihydro-2'-quinone and 10,11-dihydroxydihydro-O-desmethylquinine. These secondary metabolites probably arose from further biotransformation of the four primary metabolites.
Bannon
P
P
Département de Biochimie, Centre hospitalier de l'Université de Montréal, Campus Saint-Luc, Québec, Canada.
Yu
P
P
Cook
J M
JM
Roy
L
L
Villeneuve
J P
JP
D000284:Administration, Oral; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D011803:Quinine / Q000008:administration & dosage / Q000652:urine*
D011803:Quinine