Identification and chromosomal assignment of USP1, a novel gene encoding a human ubiquitin-specific protease.
We have cloned a novel gene encoding a human ubiquitin-specific protease (USP1). The product, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases. Recombinant USP1 protein showed genuine UBP activity, correctly cleaving Ub-beta-galactosidase to produce ubiquitin and beta-galactosidase. Chromosomal mapping by fluorescence in situ hybridization and radiation hybrid analyses localized the USP1 gene to the p31.3-p32.1 band of chromosome 1. As losses of heterozygosity or amplifications have been observed in the distal region of the short arm of chromosome 1 in some neuroblastomas, breast cancers, and pancreatic adenocarcinomas, the USP1 gene may be a candidate for either the tumor-suppressive or the oncogenic activities associated with that chromosomal region.
Fujiwara
T
T
Otsuka GEN Research Institute, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno Kawauchi-cho, Tokushima, 771-0192, Japan. tfuji@otsuka.gr.jp
Saito
A
A
Suzuki
M
M
Shinomiya
H
H
Suzuki
T
T
Takahashi
E
E
Tanigami
A
A
Ichiyama
A
A
Chung
C H
CH
Nakamura
Y
Y
Tanaka
K
K
D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D018076:DNA, Complementary; D010450:Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA; D064570:Ubiquitin-Specific Proteases
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D011994:Recombinant Proteins; D010450:Endopeptidases; C579228:USP1 protein, human; D064570:Ubiquitin-Specific Proteases