[The identification and isolation of Toxoplasma gondii antigens].
It is know that 30% of AIDS patients may develop toxoplasmic encephalitis in an advanced stage of the disease. This study was conducted to investigate the presence of antigens to Toxoplasma gondii in the urine of patients infected by human immunodeficiency virus, group IV, who presenting with acute toxoplasmosis. These proteins were identified by electrophoresis in polyacrylamide gels, in the presence of sodium dodecyl sulfate in reduction conditions with the subsequent transfer to nitrocellulose sheets. Finally, they were isolated by affinity chromatography. Proteins with molecular weights nearly 66 and 61 KD were identified in the samples studied. Results obtained from this study highlights the presence of Toxoplasma gondii circulating antigens in patient presenting with AIDS and toxoplasmic encephalitis. The detection of these antigens in the urine of patients would be a very useful tool for the diagnosis of the acute infection by this protozoon.
Fonseca Géigel
L
L
Instituto de Medicina Tropical Pedro Kourí.
Fachado Carvajales
A
A
D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis; D000818:Animals; D000953:Antigens, Protozoan / Q000302:isolation & purification / Q000652:urine*; D015153:Blotting, Western / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D015497:HIV-1; D006801:Humans; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits; D014122:Toxoplasma / Q000276:immunology*; D016781:Toxoplasmosis, Cerebral / Q000175:diagnosis
D000953:Antigens, Protozoan