title: Isoforms of murine and human serum amyloid P component.
abstract: Isoelectric focusing (IEF) and immunofixation of murine serum amyloid P component (SAP), purified and in serum, showed a distinct and strain-dependent isoform pattern with up to seven bands (pI 5.1-5.7). Neuraminidase treatment caused a shift of the isoforms to more basic pI values, but did not affect their number. When the acute-phase response was analysed in three mouse strains, CBA/J and C3H/HeN initially showed seven SAP isoforms in serum and C57BL/6 J three or four. The responses in all three strains peaked at day 2 and were normalized within 14 days. On days 2 and 4, CBA/J and C3H/HeN mice showed one more acidic isoform and an increase in the concentration of the most basic isoform. C57BL/6 J mice exhibited two to three new isoforms during the acute-phase response. This appears to be the first demonstration of the physiological existence of SAP isoforms. In contrast, demonstration of isoforms of human SAP required the presence of urea and higher SAP concentrations. TEF and immunofixation of SAP monomers showed five to eight isoforms, ranging from pI 4.7-5.7. IEF of SAP in human serum resulted in a less distinct pattern and more acidic isoforms. As with murine SAP, neuraminidase treatment caused a shift of the isoforms, but no reduction in isoform number. Two-dimensional gel electrophoresis confirmed the existence of multiple isoforms of human SAP monomers.
authors.affiliation: Department of Medical Microbiology, Odense University, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Nybo
authors.forename: R
authors.initials: R
authors.lastname: Hackler
authors.forename: B
authors.initials: B
authors.lastname: Kold
authors.forename: E H
authors.initials: EH
authors.lastname: Nielsen
authors.forename: A
authors.initials: A
authors.lastname: Steinmetz
authors.forename: S E
authors.initials: SE
authors.lastname: Svehag
mesh_terms: D000818:Animals; D006801:Humans; D007525:Isoelectric Focusing; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D020033:Protein Isoforms; D000683:Serum Amyloid P-Component / Q000737:chemistry* / Q000378:metabolism
chemical_list: D020033:Protein Isoforms; D000683:Serum Amyloid P-Component