title: Antibody repertoire development in fetal and neonatal piglets. I. Four VH genes account for 80 percent of VH usage during 84 days of fetal life.
abstract: VDJ rearrangement and VH gene usage during fetal development in 35 outbred piglets was examined by PCR amplification of VDJs; VDJs were subsequently characterized by hybridization with VH-specific gene probes and by sequencing. VDJ rearrangement was first seen in the fetal liver on day 30 of a 114-day gestation. Four VH genes (V(H)A, V(H)B, V(H)C, and V(H)E) accounted for approximately 80% of all VH gene usage regardless of gestational age, choice of piglet, or lymphoid tissue tested; D(H)A and D(H)B were used in >90% of the fetal VDJs examined. Evidence of somatic hypermutation during fetal development was not found. The proportion of the four prominent fetal VH genes did not differ significantly between cDNA and DNA, suggesting the absence of selective B cell differentiation. A comparison of recombination signal sequences, flanking sequences, and framework sequences of these fetal genes with other germline VH genes of swine offered no clue as to their selective usage. N-region additions were prominent on day 40 but not on day 30, suggesting that the onset of terminal deoxynucleotidyltransferase activity occurs after 30 days of fetal development. These collective findings indicate that the preimmune, "natural Ab" repertoire of the fetal piglet is largely restricted to the use of four nonpolymorphic and nonmutated VH genes and two nonmutated DH segments. This suggests that the preimmune repertoire of swine is either highly restricted or almost entirely determined by junctional diversity in complementarity-determining region-3.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City 52242-1109, USA.
authors.forename: J
authors.initials: J
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Hayward
authors.forename: R
authors.initials: R
authors.lastname: Shinde
authors.forename: R
authors.initials: R
authors.lastname: Christenson
authors.forename: S P
authors.initials: SP
authors.lastname: Ford
authors.forename: J E
authors.initials: JE
authors.lastname: Butler
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000916:Antibody Diversity / Q000502:physiology; D001483:Base Sequence; D016384:Consensus Sequence; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D005333:Fetus / Q000276:immunology*; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D005865:Gestational Age; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D008221:Lymphoid Tissue / Q000196:embryology / Q000276:immunology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013552:Swine / Q000196:embryology / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D045567:VDJ Recombinases
chemical_list: D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D004254:DNA Nucleotidyltransferases; D045567:VDJ Recombinases