title: The role of biomineralization in microbiologically influenced corrosion.
abstract: Synthetic iron oxides (goethite, alpha-FeO.OH; hematite, Fe2O3; and ferrihydrite, Fe(OH)3) were used as model compounds to simulate the mineralogy of surface films on carbon steel. Dissolution of these oxides exposed to pure cultures of the metal-reducing bacterium, Shewanella putrefaciens, was followed by direct atomic absorption spectroscopy measurement of ferrous iron coupled with microscopic analyses using confocal laser scanning and environmental scanning electron microscopies. During an 8-day exposure the organism colonized mineral surfaces and reduced solid ferric oxides to soluble ferrous ions. Elemental composition, as monitored by energy dispersive x-ray spectroscopy, indicated mineral replacement reactions with both ferrihydrite and goethite as iron reduction occurred. When carbon steel electrodes were exposed to S. putrefaciens, microbiologically influenced corrosion was demonstrated electrochemically and microscopically.
authors.affiliation: Naval Research Laboratory, Stennis Space Center, MS 39529, USA.
authors.forename: B
authors.initials: B
authors.lastname: Little
authors.forename: P
authors.initials: P
authors.lastname: Wagner
authors.forename: K
authors.initials: K
authors.lastname: Hart
authors.forename: R
authors.initials: R
authors.lastname: Ray
authors.forename: D
authors.initials: D
authors.lastname: Lavoie
authors.forename: K
authors.initials: K
authors.lastname: Nealson
authors.forename: C
authors.initials: C
authors.lastname: Aguilar
mesh_terms: D001673:Biodegradation, Environmental; D002244:Carbon; D003343:Corrosion; D004566:Electrodes; D005290:Ferric Compounds / Q000737:chemistry / Q000378:metabolism; D005293:Ferritins / Q000737:chemistry / Q000378:metabolism; D006092:Gram-Negative Facultatively Anaerobic Rods / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D058085:Iron Compounds / Q000737:chemistry / Q000378:metabolism; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008903:Minerals; D010084:Oxidation-Reduction; D012965:Sodium Chloride / Q000737:chemistry; D013232:Steel
chemical_list: D005290:Ferric Compounds; D058085:Iron Compounds; D008903:Minerals; D013232:Steel; C094886:goethite; C000499:ferric oxide; D012965:Sodium Chloride; D002244:Carbon; C092844:ferric oxyhydroxide; D005293:Ferritins; D007501:Iron