Effect of gadolinium chloride treatment on concanavalin A-induced cytokine mRNA expression in mouse liver.
The effect of Kupffer cell depression on concanavalin A (Con A)-induced cytokine mRNA expression in the liver was studied. Gadolinium chloride (GdCl3) is a commonly used Kupffer cell inhibitor. GdCl3 (40 mg/kg, i.p.) was injected into each mouse, and 24 hr later, Con A (0.2 mg/mouse) was administered. Plasma was obtained at 24 hr after Con A treatment for alanine aminotransferase (ALT) measurement. GdCl3 treatment inhibited Con A-induced elevation of ALT. However, it did not inhibit Con A-induced interleukin-2 or tumor necrosis factor-alpha mRNA expression. The present results suggest that Kupffer cells are not responsible for Con A-induced cytokine expression in the liver.
Okamoto
T
T
Research Laboratories, Nippon Chemiphar Co., Ltd., Misato, Saitama, Japan.
Maeda
O
O
Tsuzuike
N
N
Hara
K
K
D000410:Alanine Transaminase / Q000097:blood / Q000187:drug effects; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D016207:Cytokines / Q000187:drug effects* / Q000235:genetics; D005260:Female; D005682:Gadolinium / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007376:Interleukin-2 / Q000235:genetics; D007728:Kupffer Cells / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000187:drug effects* / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000235:genetics
D000893:Anti-Inflammatory Agents; D016207:Cytokines; D007376:Interleukin-2; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D005682:Gadolinium; D000410:Alanine Transaminase; C038958:gadolinium chloride