title: Effects of neurotransmitters, gut hormones, and inflammatory mediators on mucus discharge in rat colon.
abstract: The effect of potential mediators of mucus secretion was investigated in the isolated vascularly perfused rat colon by using a sandwich enzyme-linked immunosorbent assay for rat colonic mucin and by histochemical analysis. Bethanechol (100-200 microM), bombesin (100 nM), and vasoactive intestinal peptide (VIP, 100 nM) provoked a dramatic mucin discharge (maximal response at 900, 900, and 600% of control loops, respectively). VIP-stimulated mucin secretion was abolished by tetrodotoxin, whereas atropine was without effect. In contrast, both tetrodotoxin and atropine significantly decreased mucin release induced by bombesin. Isoproterenol or calcitonin gene-related peptide was without effect. Serotonin (1-5 microM) and peptide YY (10 nM) evoked mucin discharge, whereas glucagon-like peptide-1 did not release mucin. Finally, bromolasalocid (20 microM), interleukin-1beta (0.25 nM), sodium nitroprusside (1 mM), and dimethyl-PGE2 (2.5 microM) induced mucus discharge. The results demonstrated a good correlation between the immunological method and histological analysis. In conclusion, these findings suggest a role for the enteric nervous system, the enteroendocrine cells, and resident immune cells in mediation of colonic mucus release.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale U-45, Hôpital Edouard Herriot, 69347 Lyon Cedex 03, France.
authors.forename: P
authors.initials: P
authors.lastname: Plaisancié
authors.forename: A
authors.initials: A
authors.lastname: Barcelo
authors.forename: F
authors.initials: F
authors.lastname: Moro
authors.forename: J
authors.initials: J
authors.lastname: Claustre
authors.forename: J A
authors.initials: JA
authors.lastname: Chayvialle
authors.forename: J C
authors.initials: JC
authors.lastname: Cuber
mesh_terms: D015064:16,16-Dimethylprostaglandin E2 / Q000494:pharmacology; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D018723:Bethanechol / Q000494:pharmacology; D001839:Bombesin / Q000494:pharmacology; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D003106:Colon / Q000502:physiology*; D017615:Enteric Nervous System / Q000187:drug effects / Q000502:physiology; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D005934:Glucagon / Q000494:pharmacology; D052216:Glucagon-Like Peptide 1; D007249:Inflammation; D007375:Interleukin-1 / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D007545:Isoproterenol / Q000494:pharmacology; D007832:Lasalocid / Q000031:analogs & derivatives / Q000494:pharmacology; D008297:Male; D009093:Mucus / Q000187:drug effects / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D019894:Peptide YY / Q000494:pharmacology; D011498:Protein Precursors / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000494:pharmacology; D013779:Tetrodotoxin / Q000494:pharmacology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D005768:Gastrointestinal Hormones; D007375:Interleukin-1; D018377:Neurotransmitter Agents; D010446:Peptide Fragments; D011498:Protein Precursors; D018723:Bethanechol; D019894:Peptide YY; D009599:Nitroprusside; D012701:Serotonin; D014660:Vasoactive Intestinal Peptide; D013779:Tetrodotoxin; D001285:Atropine; D052216:Glucagon-Like Peptide 1; D005934:Glucagon; D015740:Calcitonin Gene-Related Peptide; D007545:Isoproterenol; D015064:16,16-Dimethylprostaglandin E2; D001839:Bombesin; C029086:bromolasalocid; D007832:Lasalocid