Atrial natriuretic factor does not affect norepinephrine catabolism in rat hypothalamus and adrenal medulla.
We have previously reported that atrial natriuretic factor (ANF) increases neuronal uptake and endogenous content of norepinephrine (NE) and diminishes neuronal release, synthesis and turn-over of NE in rat hypothalamus and adrenal medulla. The aim of the present work was to study another aspect of NE metabolism and therefore investigate the possible effects of ANF on NE catabolism. The determination of monoamine oxidase (MAO) and catechol-O-methyl transferase (COMT) activity and deaminates metabolites formation were studied in vitro in rat hypothalamus and adrenal medulla slices. Results showed that, in the hypothalamus, 100 nM ANF diminished MAO activity while 10 nM ANF did not modify the enzyme activity. Conversely, 10 and 100 nM ANF reduced MAO activity in adrenal medulla. On the other hand, the atrial factor modified neither COMT activity nor the formation of deaminates metabolites in the hypothalamus and adrenal medulla. Present results as well as previous findings support a putative role for ANF in the modulation of NE metabolism not only in the hypothalamus but also in the adrenal medulla of the rat, affecting the storage, release and uptake of NE but not its catabolism.
Vatta
M S
MS
Catedras de Fisiologia y Fisiopatologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina. mvatta@ffyb.uba.ar
Rubio
M
M
Bianciotti
L G
LG
Fernandez
B E
BE
D000313:Adrenal Medulla / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000502:physiology*; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D007031:Hypothalamus / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008333:Mandelic Acids / Q000032:analysis; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000032:analysis; D008995:Monoamine Oxidase / Q000378:metabolism; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism*; D009647:Normetanephrine / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar
D008333:Mandelic Acids; C472610:atrial natriuretic peptide, rat; D009647:Normetanephrine; D008734:Methoxyhydroxyphenylglycol; C000989:3,4-dihydroxymandelic acid; D009320:Atrial Natriuretic Factor; D008995:Monoamine Oxidase; D002394:Catechol O-Methyltransferase; C010117:3,4-dihydroxyphenylglycol; D009638:Norepinephrine