Establishment of the enzyme-linked immunosorbent assay for connective tissue growth factor (CTGF) and its detection in the sera of biliary atresia.
Connective tissue growth factor (CTGF) is a mitogenic, chemotactic, and cell matrix-inducing factor for fibroblasts. We generated murine monoclonal antibodies against CTGF and established a sandwich enzyme-linked immunosorbent assay (ELISA) for detection of CTGF. By using the ELISA, we confirmed that CTGF was specifically induced in human fibroblasts by TGF-beta but not by PDGF, FGF, IGF-I, or EGF. We also found that the serum levels of CTGF were significantly correlated with the progression of hepatic fibrosis in biliary atresia. These results indicated that CTGF is potentially a useful parameter for monitoring certain types of fibrotic disorders.
Tamatani
T
T
Pharmaceutical Frontier Research Laboratories, JT Inc., Yokohama, Kanagawa, 236-0004, Japan. tamatani@ikrl.jti.co.jp
Kobayashi
H
H
Tezuka
K
K
Sakamoto
S
S
Suzuki
K
K
Nakanishi
T
T
Takigawa
M
M
Miyano
T
T
D001656:Biliary Atresia / Q000097:blood* / Q000150:complications; D055513:Connective Tissue Growth Factor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005347:Fibroblasts / Q000187:drug effects; D006133:Growth Substances / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D008103:Liver Cirrhosis / Q000150:complications; D008111:Liver Function Tests; D016037:Single-Blind Method; D016211:Transforming Growth Factor alpha / Q000096:biosynthesis
C526317:CCN2 protein, human; D006133:Growth Substances; D017874:Immediate-Early Proteins; D036341:Intercellular Signaling Peptides and Proteins; D016211:Transforming Growth Factor alpha; D055513:Connective Tissue Growth Factor