title: Serology versus ARMS-PCR in prospective HLA-class I typing for bone marrow transplantation.
abstract: While HLA class II alleles identification by means of complement mediated lymphocytotoxicity (serology) is almost replaced by DNA typing techniques, serology is still widely used for routine class I typing. The aim of this prospective study was to compare PCR-based Amplification Refractory Mutation System with serology in clinical HLA class I alleles assignment in patients receiving marrow transplants and their potential donors. The total discrepancy rate in 114 consecutively typed individuals for HLA-A and HLA-C alleles was only in favor of ARMS-PCR, whereas HLA-B typing was discrepant also in favor of serology. The discrepancies were higher in patients, particularly in those with acute lymphoblastic leukaemia, than in healthy individuals. We conclude, that ARMS-PCR is clearly superior to serology in definition of class I alleles, which might be of clinical importance particularly for bone marrow transplantation.
authors.affiliation: Cardiovascular Surgery Center of Dresden, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Usichenko
authors.forename: A
authors.initials: A
authors.lastname: Lattermann
authors.forename: G
authors.initials: G
authors.lastname: Mueller-Eckhardt
mesh_terms: D000293:Adolescent; D000328:Adult; D020137:Base Pair Mismatch; D016026:Bone Marrow Transplantation / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007223:Infant; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011446:Prospective Studies; D015203:Reproducibility of Results; D012698:Serologic Tests
chemical_list: D015395:Histocompatibility Antigens Class I