TAFII mutations disrupt Dorsal activation in the Drosophila embryo.
In this study, we present evidence that the Dorsal activator interacts with limiting amounts of the TFIID complex in the Drosophila embryo. In vitro transcription reactions and protein binding assays implicate the TAFII110 and TAFII60 subunits of the TFIID complex in contributing to Dorsal-mediated activation. Mutations in TAFII110 and TAFII60 result in altered patterns of snail and twist transcription in embryos derived from dl/+ females. These results suggest that TAFIIs contribute to the activation of transcription in vivo and support the hypothesis that subunits of TFIID may serve as targets of enhancer binding proteins.
Zhou
J
J
Molecular and Cell Biology Department, Molecular and Cell Biology Department, University of California, Berkeley, 401 Barker Hall, Berkeley, CA 94720-3204, USA.
Zwicker
J
J
Szymanski
P
P
Levine
M
M
Tjian
R
R
D000818:Animals; D004330:Drosophila / Q000196:embryology / Q000235:genetics*; D029721:Drosophila Proteins; D018507:Gene Expression Regulation, Developmental; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D010750:Phosphoproteins / Q000235:genetics*; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII / Q000235:genetics*; D015533:Transcriptional Activation
D029721:Drosophila Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C062236:dl protein, Drosophila