Capillary chromatography/microelectrospray mass spectrometry used for the identification of putative cyclin-dependent kinase inhibitory protein in Medicago.
A home-built capillary chromatography/microelectrospray system was used for peptide mass mapping of a putative cyclin-dependent kinase inhibitor protein with molecular mass of 8.5 kDa. The masses of identified tryptic fragments were then input to a protein database search routine. Daughter ion scans were done only on the most abundant tryptic fragments. On the basis of protein database search results and tandem mass spectrometric measurements the bioactive protein was identified as ubiquitin.
Kele
Z
Z
Department of Medical Chemistry, Albert Szent-Györgyi Medical University, Hungary.
Janáky
T
T
Mészáros
T
T
Fehér
A
A
Dudits
D
D
Szabó
P T
PT
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D019075:Electrophoresis, Capillary; D004791:Enzyme Inhibitors / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D013058:Mass Spectrometry; D000455:Medicago sativa / Q000737:chemistry*; D008869:Microtubule-Associated Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
C108573:CdkI-p30 protein, Cricetulus griseus; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D004791:Enzyme Inhibitors; D008869:Microtubule-Associated Proteins; D010446:Peptide Fragments; D018844:Cyclin-Dependent Kinases; D014357:Trypsin