Protective effects of the amino acid glutamine and of ascorbic acid against chromosomal damage induced by doxorubicin in mammalian cells.
The interaction of antioxidants can provide an essential protection against the damaging effects of free radicals. Beneficial interactions include radioprotection, protection against acute toxicity of chemicals, and antimutagenic and anticarcinogenic activity. The present study was undertaken to evaluate the protective effect of the amino acid glutamine (GLN) and ascorbic acid (AA) on the frequency of chromosomal aberrations induced by the antineoplastic agent doxorubicin (DXR). These micronutrients were tested separately and simultaneously in Wistar rat bone marrow and Chinese hamster ovary (CHO) cells. The treatments with GLN and/or AA significantly decreased the frequency of DXR-induced clastogenic damage in both test systems.
Tavares
D C
DC
Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Brasil. dtavares@spider.usp.br
Cecchi
A O
AO
Antunes
L M
LM
Takahashi
C S
CS
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000633:toxicity*; D001205:Ascorbic Acid / Q000494:pharmacology*; D016466:CHO Cells; D002869:Chromosome Aberrations; D006224:Cricetinae; D004317:Doxorubicin / Q000633:toxicity*; D005260:Female; D005973:Glutamine / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000903:Antibiotics, Antineoplastic; D005973:Glutamine; D004317:Doxorubicin; D001205:Ascorbic Acid