title: Bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep.
abstract: OBJECTIVE
  To study the clinical signs following bluetongue virus serotypes 1 and 3 infection in Poll Dorset sheep.
  
  
  METHODS
  A clinical and pathological study.
  
  
  METHODS
  Twenty Poll Dorset sheep were inoculated with bluetongue virus serotypes 1 or 3, each inoculum having a different passage history. The sheep were examined daily and their clinical appearance and rectal temperatures recorded. Heparinised and non-heparinised blood samples were taken at intervals for virological and serological study. Gross pathological findings were recorded for several sheep at necropsy and tissue samples were collected from three sheep for virological studies.
  
  
  RESULTS
  All inoculated sheep developed clinical disease. The clinical signs and gross pathological changes varied considerably but were consistent with damage to the vascular endothelial system. There was a decline in the titres of infectious bluetongue virus and of antigen in tissues collected between 7 and 12 days after infection.
  
  
  CONCLUSIONS
  The severity of disease was related to the speed of onset and duration of pyrexia and not the development or titre of viraemia. Generally, those animals with sensitive mouths, depression, coronitis, recumbency and reluctance to move were the most debilitated. Whole blood was the most reliable source of infectious virus from acutely and chronically infected and convalescent animals. However, tissue samples particularly spleen, collected from dead or killed animals suffering from either peracute or acute forms of disease were most appropriate for the rapid confirmation of a clinical diagnosis.
authors.affiliation: Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
authors.forename: C
authors.initials: C
authors.lastname: Hamblin
authors.forename: J S
authors.initials: JS
authors.lastname: Salt
authors.forename: S D
authors.initials: SD
authors.lastname: Graham
authors.forename: K
authors.initials: K
authors.lastname: Hopwood
authors.forename: A M
authors.initials: AM
authors.lastname: Wade-Evans
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000956:Antigens, Viral / Q000032:analysis; D001344:Autopsy / Q000662:veterinary; D001819:Bluetongue / Q000276:immunology / Q000473:pathology / Q000821:virology*; D001820:Bluetongue virus / Q000145:classification* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D012692:Serial Passage / Q000662:veterinary; D012703:Serotyping / Q000662:veterinary; D012756:Sheep; D014766:Viremia / Q000276:immunology / Q000662:veterinary* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral