Role of serum in regulating the Entamoeba histolytica cell cycle: a flowcytometric analysis.
To identify stage-specific molecules that might be important in pathogenicity, we focused on synchronizing Entamoeba histolytica HM1:1MSS axenic cultures by serum deprivation. By growing trophozoites in medium containing 2% serum for 16 h and then replacing with complete medium, we found that most of the trophozoites were in the G0/G1 phase of the cell cycle. Subsequently, the distribution of trophozoites in G0/G1, S and G2/M phases was studied for up to 24 h and it was observed that after 8 h, the trophozoites began to come out of the quiescent G0/G1 phase and the S and G2/M populations increased, indicating that certain serum factors regulate induction of cell division. It will be important to investigate these factors to find serum substitutes for cultivation media.
Vohra
H
H
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Mahajan
R C
RC
Ganguly
N K
NK
D000818:Animals; D001790:Blood Physiological Phenomena; D016895:Culture Media, Serum-Free; D004748:Entamoeba histolytica / Q000254:growth & development*; D005434:Flow Cytometry; D007399:Interphase; D008018:Life Cycle Stages; D008938:Mitosis; D016196:S Phase
D016895:Culture Media, Serum-Free