Comparative study on the inhibitory effect of recombinant FN polypeptide CH50 and CH56 on the metastasis of melanoma cells.
On the basis of preparation of anti-metastatic recombinant FN polypeptides, CH50 and CH56, we further studied the function of these polypeptides. The capacity of CH50 binding with melanoma cells (ED50 30 mM) was higher than that of CH56 (ED50 45 mM). Both of the polypeptides could significantly suppress the binding of melanoma B16 cells to laminin. There was no significant difference in the inhibitory effect between two polypeptides. In the experimental metastasis of melanoma cells, both of CH50 and CH56 could significantly inhibit the metastasis of the tumor cells, and reduce the number of lung metastasis by about 80%. Our results suggest that III-11 and ED-A repeats influenced, to some extent, the binding capacity of bifunctional-domain polypeptide to cells, but did not affect the inhibition of the polypeptide on the metastasis of melanoma cells. The presence and connection of cell I and Hep II domains are the elements which determine the ability of recombinant FN polypeptides to inhibit the metastasis of tumor cells.
Zhang
G
G
Department of Medical Molecular Biology, Tongji Medical University, Wuhan.
Feng
Z
Z
Zhang
H
H
Li
D
D
Fan
Q
Q
D000818:Animals; D005353:Fibronectins / Q000494:pharmacology*; D007797:Laminin / Q000378:metabolism; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology*; D051379:Mice; D009362:Neoplasm Metastasis / Q000517:prevention & control*; D010455:Peptides / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D005353:Fibronectins; D007797:Laminin; D010455:Peptides; D011994:Recombinant Proteins