title: Identification of a Torpedo homolog of Sam68 that interacts with the synapse organizing protein rapsyn.
abstract: Nicotinic acetylcholine receptors (nAChRs) are initially expressed diffusely on the surface of myotubes and, in response to neuronally derived factors, cluster at the endplate to a final concentration of approximately 10000/microm2. The synaptic peripheral membrane protein rapsyn has been shown to mediate clustering of nAChRs in several systems. Here we describe the use of the yeast two-hybrid system to identify proteins that can interact with rapsyn. One of the clones we have identified is a Torpedo californica homolog of the Src-associated in mitosis protein (Sam68). We further show that Sam68, like rapsyn, is localized at the neuromuscular junction.
authors.affiliation: Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: E T
authors.initials: ET
authors.lastname: Fung
authors.forename: A
authors.initials: A
authors.lastname: Lanahan
authors.forename: P
authors.initials: P
authors.lastname: Worley
authors.forename: R L
authors.initials: RL
authors.lastname: Huganir
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism*; D009469:Neuromuscular Junction / Q000737:chemistry; D016601:RNA-Binding Proteins / Q000302:isolation & purification / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism*; D014101:Torpedo
chemical_list: D009124:Muscle Proteins; D016601:RNA-Binding Proteins; D011978:Receptors, Nicotinic; C051609:peripheral membrane protein 43K