title: Construction and characterization of hexon-chimeric adenoviruses: specification of adenovirus serotype.
abstract: This study has used the strategy of gene replacement to characterize the contribution of the adenovirus (Ad) capsid protein hexon to serotype definition. By replacing the Ad type 5 (Ad5) hexon gene with sequences from Ad2, we have changed the type specificity of the chimeric virus. The type-determining epitopes are primarily associated with loop 1 of hexon and, to a much lesser degree, with loop 2. In spite of the serotype distinctiveness of the chimeric hexon viruses, epitope similarity between the vectors resulted in a low level of cross-reactive neutralizing antibody, which in combination with activated cellular and innate arms of the immune system is sufficient to suppress gene transduction following readministration in vivo.
authors.affiliation: Department of Microbiology, W. R. Hearst Research Foundation, Cornell University Medical College, New York, New York 10021, USA.
authors.forename: J G
authors.initials: JG
authors.lastname: Gall
authors.forename: R G
authors.initials: RG
authors.lastname: Crystal
authors.forename: E
authors.initials: E
authors.lastname: Falck-Pedersen
mesh_terms: D000257:Adenoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000235:genetics; D002213:Capsid / Q000235:genetics* / Q000276:immunology*; D036022:Capsid Proteins; D002460:Cell Line; D002678:Chimera / Q000235:genetics* / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes / Q000235:genetics; D005814:Genes, Viral; D005822:Genetic Vectors; D006801:Humans; D017921:Mastadenovirus / Q000145:classification* / Q000235:genetics* / Q000276:immunology; D009500:Neutralization Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D011995:Recombination, Genetic; D012703:Serotyping; D014161:Transduction, Genetic
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D036022:Capsid Proteins; D000939:Epitopes; C018315:hexon capsid protein, Adenovirus