A polymerase chain reaction (PCR) method for the identification of collagen adhesin gene (CNA) in Staphylococcus-induced prosthesis infections.
Staphylococci are well-recognized pathogens of foreign body-associated infections. The pathogenesis of such infections involves an initial step of contact between the colonizing bacterium and the biomaterial, with subsequent colony formation. Several studies have been devoted to identify adhesion mechanisms for these bacteria. Slime in particular has been extensively investigated. Recently, considerable attention has been given to the host protein receptors that have been shown in in vitro assays to serve as substrates for bacterial adherence. To determine the importance of the collagen adhesin as virulence factor in Staphylococcus-induced prosthesis infection, a simple and reliable method using a polymerase chain reaction (PCR) was devised to identify collagen adhesin gene (cna). By using lysates of ten strains from orthopedic prostheses (5 Staphylococcus aureus and 5 Staphylococcus epidermidis) and two 20-oligonucleotides as primers, a 192-bp region of the cna gene was amplified by PCR and detected by agarose gel electrophoresis. Results obtained by this method were in accordance with those obtained by the in vitro phenotypic characterization of binding ability to collagen of Staphylococcus strains.
Montanaro
L
L
Laboratorio di Biocompatibilità dei Materiali da Impianto, Istituti Ortopedici Rizzoli, Bologna, Italy.
Arciola
C R
CR
Borsetti
E
E
Brigotti
M
M
Baldassarri
L
L
D018829:Adhesins, Bacterial / Q000235:genetics* / Q000276:immunology; D001422:Bacterial Adhesion / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics* / Q000276:immunology; D001672:Biocompatible Materials; D017931:DNA Primers / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D006622:Hip Prosthesis / Q000009:adverse effects / Q000382:microbiology; D006801:Humans; D007720:Knee Prosthesis / Q000009:adverse effects / Q000382:microbiology; D016133:Polymerase Chain Reaction; D016459:Prosthesis-Related Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000276:immunology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity; D013212:Staphylococcus epidermidis / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D001672:Biocompatible Materials; D017931:DNA Primers; D004269:DNA, Bacterial; C048165:adhesin, Staphylococcus aureus