The growing cell plate of higher plants is a site of both actin assembly and vinculin-like antigen recruitment.
Compelling evidence supports the idea that actin filaments play an active role in the cytokinetic process of higher plant cells. However, the mechanisms that control the growth of the cell plate and its stabilization remain so far unknown. We show that a novel population of short actin filaments continuously assembles in the phragmoplast at the growing cell plate. Microinjection of rhodamine-phalloidin during these final stages of telophase revealed the dynamic assembly and organization of these actin filaments during vesicle fusion. Comparable data were obtained in endosperm syncytia during the development of the cell plate between non sister nuclei, i.e. independently of the formation of the mitotic phragmoplast. Concomitantly, plant polypeptides sharing epitopes with human vinculin are revealed within the forming cell plate, suggesting their recruitment during cytokinesis-associated actin assembly. These vinculin-like antigens may participate in membrane/F-actin anchorage protein complexes. Our data, in addition to the identification of plant integrin homologues reported by several authors, suggest the existence of a cell wall/extracellular matrix/plasma membrane/actin cytoskeleton continuum. Such an architecture may control cell-cell interactions during cell plate formation and may contribute to the establishment of polarity in higher plants.
Endlé
M C
MC
Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique UPR 406, Université Louis Pasteur, Strasbourg, France.
Stoppin
V
V
Lambert
A M
AM
Schmit
A C
AC
D000199:Actins / Q000302:isolation & purification* / Q000378:metabolism; D000941:Antigens / Q000302:isolation & purification / Q000378:metabolism; D002455:Cell Division; D007150:Immunohistochemistry; D007425:Intracellular Membranes / Q000648:ultrastructure; D019684:Magnoliopsida / Q000648:ultrastructure*; D008954:Models, Biological; D008961:Models, Structural; D016596:Vinculin / Q000302:isolation & purification* / Q000378:metabolism
D000199:Actins; D000941:Antigens; D016596:Vinculin