Mouse cortical neurones lacking APP show normal neurite outgrowth and survival responses in vitro.
We have cultured neurones from the developing cortex of mice that have had the amyloid precursor protein gene deleted (APP-null). Neurones cultured for a period of 24 h show similar neurite outgrowth and survival responses to wild-type neurones. Similar neurite outgrowth responses were also seen when neurones from APP-null mice were treated with a neurotrophic peptide derived from the APP sequence and compared with wild-type neurones. Finally, cortical cultures derived from APP-null mice showed similar survival responses to the toxic amyloid-beta peptide.
Harper
S J
SJ
Department of Pharmacology, Merck, Sharp & Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK.
Bilsland
J G
JG
Shearman
M S
MS
Zheng
H
H
Van der Ploeg
L
L
Sirinathsinghji
D J
DJ
D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology; D016564:Amyloid beta-Protein Precursor / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects
D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor