title: Perforated patch recording with beta-escin.
abstract: Perforated patch recording with nystatin, amphotericin B and gramicidin can be more difficult in the hands of some investigators than others. In addition, it is difficult to introduce low molecular weight substances such as dyes into the cytoplasm in such experiments. We have determined that beta-escin represents a convenient, easy-to-use alternative to less water-soluble ionophores.
authors.affiliation: Department of Physiology and Biophysics, University of Texas Medical Branch, Galveston, Texas, TX 77555-0641, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Fan
authors.forename: P
authors.initials: P
authors.lastname: Palade
mesh_terms: D000666:Amphotericin B; D000818:Animals; D015220:Calcium Channels / Q000502:physiology; D004553:Electric Conductivity; D017097:Electric Impedance; D004928:Escin; D005456:Fluorescent Dyes; D006096:Gramicidin; D006321:Heart / Q000502:physiology*; D007700:Kinetics; D008297:Male; D009761:Nystatin; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015220:Calcium Channels; D005456:Fluorescent Dyes; D009761:Nystatin; D006096:Gramicidin; D004928:Escin; D000666:Amphotericin B