Morphological and functional characterization of a conditionally immortalized collecting tubule cell line.
A conditionally immortalized collecting tubule cell line, mCT1, was derived from the H-2Kb-ts A58 transgenic mouse (ImmortoMouse), which harbors a temperature-sensitive mutant of the SV40 large T antigen oncogene. Cells maintained under permissive conditions [33 degreesC with interferon-gamma (IFN-gamma)] form epithelial monolayers, express large T antigen, and proliferate (>50 passages). The cells retain properties characteristic of the renal collecting tubule (CT) including: vasopressin (VP)-stimulated cAMP accumulation, aquaporin-2 expression, high transepithelial electrical resistance, VP-stimulated ion transport, and amiloride-sensitive sodium absorption. When the cells are transferred to nonpermissive conditions (39 degreesC without IFN-gamma), the steady-state level of large T antigen protein declines (>95% decrease) and cell proliferation is arrested. This conditionally immortalized, murine renal cell line should prove useful for studies of CT physiology and large T antigen biology.
Takacs-Jarrett
M
M
Departments of Pediatrics, and Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio 44106-4948, USA.
Sweeney
W E
WE
Avner
E D
ED
Cotton
C U
CU
D000818:Animals; D000957:Antigens, Viral, Tumor; D002460:Cell Line; D002472:Cell Transformation, Viral; D002712:Chlorides / Q000378:metabolism; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008822:Mice, Transgenic; D013539:Simian virus 40 / Q000276:immunology; D012964:Sodium / Q000378:metabolism
D000957:Antigens, Viral, Tumor; D002712:Chlorides; D012964:Sodium