Listeria monocytogenes infection and activation of human brain microvascular endothelial cells.
Listeria monocytogenes invasion of human brain microvascular endothelial cells (BMEC) and its role as a stimulus for endothelial cell activation were studied. Binding and invasion of intact BMEC monolayers were independent of the L. monocytogenes inlAB invasion locus. Cytochalasin D abrogated invasion of BMEC, whereas genistein effected only a 53% decrease in invasion, indicating a requirement for rearrangement of actin microfilaments but less dependence on tyrosine kinase activity. L. monocytogenes stimulated surface expression of E-selectin, ICAM-1, and to a lesser extent, VCAM-1, whereas L. monocytogenes prfA- and Deltahly mutants were severely compromised in this respect. Other experiments showed that BMEC infection stimulated monocyte and neutrophil adhesion and that CD18-mediated binding was the predominant mechanism for neutrophil adhesion to infected BMEC under static conditions. These data suggest that invasion of BMEC is a mechanism for triggering inflammation and leukocyte recruitment into the central nervous system during bacterial meningitis.
Wilson
S L
SL
Departments of Medicine, West Virginia University Health Sciences Center, Morgantown, West Virginia, USA.
Drevets
D A
DA
D008841:Actin Cytoskeleton / Q000502:physiology / Q000648:ultrastructure; D000199:Actins / Q000502:physiology / Q000648:ultrastructure; D001422:Bacterial Adhesion / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D002560:Cerebrovascular Circulation; D015638:Cytochalasin D / Q000494:pharmacology; D019040:E-Selectin / Q000235:genetics; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects / Q000382:microbiology* / Q000502:physiology*; D005786:Gene Expression Regulation; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics; D008089:Listeria monocytogenes / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D008833:Microcirculation; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
D000199:Actins; D019040:E-Selectin; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D015638:Cytochalasin D; D019833:Genistein; D011505:Protein-Tyrosine Kinases