In vitro effect of central nervous system active drugs on the ATPase-ADPase activity and acetylcholinesterase activity from cerebral cortex of adult rats.
1. The effect of several central nervous system active drugs was studied in vitro on ATPase-ADPase activity and acetylcholinesterase (AChE) activity from the cerebral cortex of adult rats. 2. Lithium (1.0-10.0 mM) had no effect on either ATPase-ADPase or acetylcholinesterase activity. 3. Imipramine (0.5-5.0 mM), desipramine (0.5-5.0 mM), amitriptyline (0.1-1.0 mM) and diazepam (0.5-2.0 mM) inhibited ATP and ADP hydrolysis at all concentrations tested. 4. AChE activity was altered by imipramine (1.0-2.0 mM) and by diazepam (0.5-2.0 mM). 5. The possible participation of ATP diphosphohydrolase and AChE in the action of these drugs cannot be ruled out. The probable reduction of ATP, ADP and acetylcholine hydrolysis by the inhibitory effect of these drugs is discussed.
Barcellos
C K
CK
Department of Biochemistry, Biosciences Institute, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Schetinger
M R
MR
Dias
R D
RD
Sarkis
J J
JJ
D000110:Acetylcholinesterase / Q000032:analysis / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000639:Amitriptyline / Q000494:pharmacology; D000818:Animals; D001081:Apyrase / Q000032:analysis / Q000378:metabolism*; D002491:Central Nervous System Agents / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects* / Q000201:enzymology*; D003891:Desipramine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D006867:Hydrolases / Q000032:analysis / Q000378:metabolism; D007099:Imipramine / Q000494:pharmacology; D066298:In Vitro Techniques; D008094:Lithium / Q000494:pharmacology; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism
D002491:Central Nervous System Agents; D000639:Amitriptyline; D008094:Lithium; D006867:Hydrolases; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases; D001081:Apyrase; D007099:Imipramine; D003975:Diazepam; D003891:Desipramine