Chromosome localization and genomic structure of the KiSS-1 metastasis suppressor gene (KISS1).
The identification and sequence of KiSS-1 (HGMW-approved symbol, KISS1), a human malignant melanoma metastasis-suppressor gene, was recently published. In this report, we present a corrected genomic sequence, genomic structure, and refined chromosomal location for KiSS-1. The genomic organization of the sequence reveals a gene consisting of four exons. The first two exons are not translated; the third exon contains 38 5' noncoding bases followed by the translational start site and another 100 translated bases. The terminal exon contains a further 332 translated bases, the translational stop codon, and the polyadenylation signal. The gene maps to chromosome 1q32 as determined by radiation hybrid mapping and FISH analysis. The relatively simple organization of this gene will facilitate analyses for mutations and abnormal expression in human tumors.
West
A
A
Department of Pathology and Laboratory Medicine and UNC-Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina, 27599, USA.
Vojta
P J
PJ
Welch
D R
DR
Weissman
B E
BE
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D018076:DNA, Complementary; D005091:Exons / Q000235:genetics; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns / Q000235:genetics; D059648:Kisspeptins; D008545:Melanoma / Q000235:genetics* / Q000556:secondary; D008969:Molecular Sequence Data; D009362:Neoplasm Metastasis / Q000235:genetics*; D011506:Proteins / Q000235:genetics*; D025521:Tumor Suppressor Proteins
D018076:DNA, Complementary; C106628:KISS1 protein, human; D059648:Kisspeptins; D011506:Proteins; D025521:Tumor Suppressor Proteins