Granulocyte-macrophage colony-stimulating factor (GM-CSF)-coated implants and their potential for reducing biomaterial-associated infection in neutropenic hosts.
The incidence of infections associated with the use of medical biomaterials is high for skin-penetrating devices, when microbes of the normal skin flora like coagulase-positive and coagulase-negative staphylococci dominate as causative organisms. The most serious ones are infections in immunocompromised individuals. A mouse model of subcutaneous staphylococcal infection yielding abscesses in cyclophosphamide-induced neutropenic mice implanted with heparinized polyethylene (H-PE) was used. The present study addresses the question of the effects of implant modification with recombinant granulocyte-macrophage stimulating factor (rGM-CSF) on the course of infection. Our findings demonstrate that such modification reduces the proliferation of bacteria within the abscess and as a consequence limits the dissemination of bacteria from the local infection induced in the neutropenic host.
Rózalska
B
B
Department of Infectious Biology, University of Lódź, Poland.
Sadowska
B
B
Ljungh
A
A
Rudnicka
W
W
D000038:Abscess / Q000382:microbiology / Q000517:prevention & control*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004195:Disease Models, Animal; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009503:Neutropenia / Q000150:complications*; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology; D011095:Polyethylenes; D011994:Recombinant Proteins; D013203:Staphylococcal Infections / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000254:growth & development*; D013212:Staphylococcus epidermidis / Q000254:growth & development*
D000900:Anti-Bacterial Agents; D011095:Polyethylenes; D011994:Recombinant Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor