Homology between human chromosome 2p13.3 and the wobbler critical region on mouse chromosome 11: comparative high-resolution mapping of STS and EST loci on YAC/BAC contigs.
Human Chr 2p13-14 and homologous regions on mouse Chrs 6 and 11 have been subjects of previous studies because they comprise the loci for several neuromuscular diseases. Here we report on high-resolution mapping of 55 STS and EST loci on human Chr 2p13.3 and of 47 markers on the corresponding region on proximal mouse Chr. 11. The maps comprise several known genes, MEIS1/Meis1, RAB1a/Rab1a, MDH1/Mor2, OTX1/Otx1, and REL on human 2p13.3 and mouse Chr 11, respectively, as well as the wobbler (wr) critical region of the mouse. Whereas a perfect correspondence was found in most of the 4-Mb region, a small rearrangement was discovered around the OTX1/Otx1 locus. The detailed STS and EST transcript maps of these regions and a further narrowing down of the mouse wr critical region to the interval between D11Mit79 and D11Mit19 allow for the selection of positional candidate genes for wr, and the exclusion of others.
Resch
K
K
Developmental Biology Unit, W7, University of Bielefeld, D-33501 Bielefeld, Germany.
Korthaus
D
D
Wedemeyer
N
N
Lengeling
A
A
Ronsiek
M
M
Thiel
C
C
Baer
K
K
Jockusch
H
H
Schmitt-John
T
T
D000818:Animals; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D019204:GTP-Binding Proteins / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008291:Malate Dehydrogenase / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000235:genetics; D009857:Oncogenes / Q000235:genetics; D051857:Otx Transcription Factors; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors; D020761:rab1 GTP-Binding Proteins
D018398:Homeodomain Proteins; C000616391:MEIS1 protein, human; C000616392:Meis1 protein, mouse; D000075064:Myeloid Ecotropic Viral Integration Site 1 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; C497655:OTX1 protein, human; D051857:Otx Transcription Factors; C497656:Otx1 protein, mouse; D014157:Transcription Factors; D008291:Malate Dehydrogenase; C514238:Mor2 protein, mouse; D019204:GTP-Binding Proteins; D020761:rab1 GTP-Binding Proteins