title: Detection of chromosomal aberrations in tumor cells and tumor infiltrating lymphocytes by molecular cytogenetics in patients with gynecological cancer.
abstract: Conventional cytogenetic studies of tumor cells from patients with breast or ovarian cancer have shown multiple chromosomal abnormalities including chromosomes 7, 12, and 17. This study was designed to analyze the cytogenetic features of tumor cells and tumor infiltrating lymphocytes (TILs) by using a combination of magnetic activated cell sorting (MACS) and fluorescence in situ hybridization (FISH). Tumor cell, peripheral blood (PB), and TIL samples from 37 patients (20 ovarian tumors, 13 breast cancers, 3 uterine sarcoma, 1 carcinoma of the filamentary tube) were analyzed for the presence of numerical aberrations of chromosomes 7, 12, and 17. All of the tumor cells showed a high frequency of numerical aberrations of chromosomes 7, 12, and 17, especially trisomies or tetrasomies. There was no statistically significant difference in the incidence of chromosomal abnormalities in tumor tissue and effusions, or between primary and relapsed disease in patients with breast or ovarian tumors. However, tumor cells from patients with solid metastatic disease had significantly higher numbers of aberrations of chromosome 7 in the primary tumor than in tumors from patients without metastases (P = 0.049), suggesting that chromosome 7 is frequently involved in the progression of disease. Monosomies and trisomies of chromosomes 7 and 12 also occurred at a low percentage of TILs without any statistically significant difference between primary and relapsed tumors. The presence of these aneuploidies might be responsible for treatment failures in the immunotherapy of gynecological cancer.
authors.affiliation: Department of Gynecology and Obstetrics, University of Cologne, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Engel
authors.forename: J
authors.initials: J
authors.lastname: Friedrich
authors.forename: C
authors.initials: C
authors.lastname: Kleespies
authors.forename: C M
authors.initials: CM
authors.lastname: Kurbacher
authors.forename: T
authors.initials: T
authors.lastname: Schöndorf
authors.forename: O
authors.initials: O
authors.lastname: Grecu
authors.forename: H
authors.initials: H
authors.lastname: Kolhagen
authors.forename: P
authors.initials: P
authors.lastname: Mallmann
mesh_terms: D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000235:genetics; D002869:Chromosome Aberrations; D002881:Chromosomes, Human, Pair 12; D002886:Chromosomes, Human, Pair 17; D002897:Chromosomes, Human, Pair 7; D005260:Female; D005833:Genital Neoplasms, Female / Q000235:genetics*; D006801:Humans; D016246:Lymphocytes, Tumor-Infiltrating / Q000648:ultrastructure*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D010051:Ovarian Neoplasms / Q000235:genetics; D014594:Uterine Neoplasms / Q000235:genetics