title: Identification of a histidine acid phosphatase (phyA)-like gene in Arabidopsis thaliana.
abstract: A close examination of the protein sequence encoded by the Arabidopsis thaliana gene F21M12.26 reveals the gene product to be a phosphomonoesterase, acid optimum (EC 3.1.3.2). A subclass of this broad acid phosphatase is also known as 'histidine acid phosphatase. ' This is the first sequence-based evidence for a 'histidine acid phosphatase' in a dicotyledon. One important member of this class of enzymes is Aspergillus niger (ficuum) phytase, which came into prominence for its commercial application as a feed additive. The putative protein from A. thaliana gene F21M12.26 shares many important features of Aspergillus phytase, namely, size, active-site sequence, catalytic dipeptide and ten cysteine residues located in the key areas of the molecule, but lacks all nine N-glycosylation sites.
authors.affiliation: Southern Regional Research Center, ARS, New Orleans, Louisiana, 70124, USA.
authors.forename: E J
authors.initials: EJ
authors.lastname: Mullaney
authors.forename: A H
authors.initials: AH
authors.lastname: Ullah
mesh_terms: D000135:Acid Phosphatase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics*; D001665:Binding Sites / Q000235:genetics; D017124:Conserved Sequence; D003545:Cysteine; D017343:Genes, Plant; D006031:Glycosylation; D006639:Histidine / Q000378:metabolism*; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D006639:Histidine; D000135:Acid Phosphatase; D003545:Cysteine