Transport of intact porphyrin by HpuAB, the hemoglobin-haptoglobin utilization system of Neisseria meningitidis.
The meningococcal hemA gene was cloned and used to construct a porphyrin biosynthesis mutant. An analysis of the hemA mutant indicated that meningococci can transport intact porphyrin from heme (Hm), hemoglobin (Hb), and Hb-haptoglobin (Hp). By constructing a HemA- HpuAB- double mutant, we demonstrated that HpuAB is required for the transport of porphyrin from Hb and Hb-Hp.
Lewis
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LA
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73103, USA. llewis@rex.uokhsc.edu
Sung
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MH
Gipson
M
M
Hartman
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K
Dyer
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DW
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D004269:DNA, Bacterial; D006242:Haptoglobins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D006242:Haptoglobins; D006454:Hemoglobins; C105625:HpuB protein, Neisseria; D008074:Lipoproteins; D011166:Porphyrins; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase