Inhibitory effects of prior low dose X-ray irradiation on Fe(3+)-NTA-induced hepatopathy in rats.
Blood activities of hepatocellular enzymes such as lactate dehydrogenase (LDH), glutamic pyruvic transaminase (GPT) and glutamic oxalacetic transaminase (GOT) peaked at 12 hours after a single intraabdominal injection of ferric nitrilotriacetate (Fe(3+)-NTA) in rats. Enzymes such as alkaline phosphatase (ALP) and leucin amino peptidase (LAP) originating in the capillary bile ducts or bile secretory liver cells were also released into the blood between 6-24 hours after intraabdominal injection of Fe(3+)-NTA in rats. Furthermore, hyperoxidation of lipids occurred in rat hepatic cell membranes, reaching a peak 6 hours after intraabdominal injection of Fe(3+)-NTA. It was found that a single prior 0.5 Gy whole body X-ray irradiation significantly increased superoxide dismutase (SOD) activities and suppressed above-mentioned symptoms of transient hepatopathy in rats.
Yamaoka
K
K
Bio-Science Department Komae Branch, Abiko Research Laboratory, Tokyo, Japan.
Nomura
T
T
Iriyama
K
K
Kojima
S
S
D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001646:Bile / Q000378:metabolism; D001652:Bile Ducts / Q000201:enzymology; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D005290:Ferric Compounds / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007931:Leucyl Aminopeptidase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects / Q000528:radiation effects; D008099:Liver / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014965:X-Rays
D005290:Ferric Compounds; D011837:Radiation-Protective Agents; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate