Enhanced immunogenicity of a recombinant genital warts vaccine adjuvanted with monophosphoryl lipid A.
The regression of genital warts is believed to be a T-cell-mediated immune effect. We have sought to enhance the immunogenicity of a therapeutic vaccine for the treatment of genital warts with the use of the adjuvant monophosphoryl lipid A (MPL-immunostimulant), a detoxified form of the lipopolysaccharide (LPS) of Salmonella minnesota R595. The comparative immunogenicity and reactogenicity of a recombinant human papillomavirus type 6 (HPV6) L2E7 fusion protein in either aqueous, oil-in-water emulsions or Alhydrogel formulations containing MPL was evaluated. We conclude that the simple addition of MPL to the L2E7 fusion protein already adsorbed onto Alhydrogel preferentially enhances antigen specific in vitro T-cell proliferative responses, IFN gamma production and in vivo delayed type hypersensitivity responses without increasing its reactogenicity.
Thompson
H S
HS
Cantab Pharmaceuticals Research Ltd, Cambridge, UK.
Davies
M L
ML
Watts
M J
MJ
Mann
A E
AE
Holding
F P
FP
O'Neill
T
T
Beech
J T
JT
Thompson
S J
SJ
Leesman
G D
GD
Ulrich
J T
JT
D000276:Adjuvants, Immunologic; D000536:Aluminum Hydroxide / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D003218:Condylomata Acuminata / Q000276:immunology / Q000517:prevention & control*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008050:Lipid A / Q000031:analogs & derivatives* / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009856:Oncogene Proteins, Viral / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015678:Viral Structural Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000956:Antigens, Viral; C073507:L2 protein, cottontail rabbit papillomavirus; D008050:Lipid A; D009856:Oncogene Proteins, Viral; D011993:Recombinant Fusion Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C062495:oncogene protein E7, Human papillomavirus type 6; D000536:Aluminum Hydroxide; D007371:Interferon-gamma; C048436:monophosphoryl lipid A