title: Determination of glutathione peroxidase activity and its contribution to hydrogen peroxide removal in erythrocytes.
abstract: A new method for the determination of glutathione peroxidase activity in erythrocytes was developed. The present method was applied to the measurement of hydrogen peroxide removal rates by glutathione peroxidase in erythrocytes at 70 microM hydrogen peroxide under simulated in vivo conditions. The removal rates by glutathione peroxidase in mouse erythrocytes were twenty-times faster than those in human ones and were 5.2 mumol/sec/g of Hb. The removal rates in acatalasemic mouse erythrocytes indicate that glutathione peroxidase is the main means of hydrogen peroxide removal in acatalasemic mouse erythrocytes. Based on these results, we concluded that glutathione peroxidase in mouse erythrocytes had sufficient ability to remove hydrogen peroxide at even relatively high concentrations. This may be one of the reasons why acatalasemic mice suffer no health problems while Japanese acatalasemic patients suffer from Takahara disease when infected with hydrogen peroxide-generating bacteria.
authors.affiliation: Department of Biochemistry, Okayama University Medical School, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Wakimoto
authors.forename: N
authors.initials: N
authors.lastname: Masuoka
authors.forename: T
authors.initials: T
authors.lastname: Nakano
authors.forename: T
authors.initials: T
authors.lastname: Ubuka
mesh_terms: D000328:Adult; D000818:Animals; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000097:blood*; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood* / Q000493:pharmacokinetics; D008658:Inactivation, Metabolic; D008297:Male; D008722:Methods; D051379:Mice; D008809:Mice, Inbred C3H; D008875:Middle Aged
chemical_list: D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase