[26S-ribonucleoprotein complex (26S-proteasome) directly interacts with fibrillar actin].
26S-complex was purified from rat liver cells by centrifugation in sucrose gradients and ion exchange chromatography. The ability of 26S-proteasome to interact with fibrillar actin (F-actin) was examined by rapid cosidementation of these particles with F-actin in isokinetic sucrose gradients. It was shown that direct interaction occurred only at a low ATP concentration.
Galkin
V E
VE
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Turoverova
L V
LV
Konstantinova
I M
IM
Pinaev
G P
GP
D000199:Actins / Q000737:chemistry*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008099:Liver / Q000737:chemistry*; D008297:Male; D010447:Peptide Hydrolases / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex; D051381:Rats
D000199:Actins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease