[Effect of adenosine on intracellular free calcium in cultured rat hippocampal CA1 neurons during anoxia].
Intracellular free Ca2+ concentration ([Ca2+]i) was measured by laser scanning confocal microscope, using Ca2+ indicator Fluo-3 in cultured hippocampal CA1 neurons isolated from newborn rat. The results showed that acute anoxia induced a rapid increase of [Ca2+]i in hippocampal CA1 neurons, and this increase could be attenuated by 100 mumol/L adenosine significantly. This effect of adenosine could be suppressed by adenosine A1 receptor antagonist CPT or potassium channel blockers, 4-AP and glipizide. These results suggest that adenosine activates 4-AP- or ATP-sensitive potassium channels through A1 receptors, and consequently inhibits the [Ca2+]i elevation in hippocampal neurons during anoxia.
Wan
Q
Q
Institute of Basic Medical Sciences, Beijing.
Wang
F Z
FZ
Yao
H
H
Liu
Z W
ZW
Huang
Y H
YH
Ding
A S
AS
D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018613:Microscopy, Confocal; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D000241:Adenosine; D002118:Calcium