PCR for detecting Pneumocystis carinii in clinical or environmental samples.
Since Pneumocystis carinii cannot be cultured in vitro, the introduction of polymerase chain reaction (PCR) has been an enormous advantage for research purposes. It is now possible to detect P. carinii in specimens containing low numbers of organisms where conventional detection methods using microscopic examination of histochemical stains has been insufficient. PCR has been used to detect P. carinii in bronchoalveolar lavage, induced sputum, spontaneous expectorates, oropharyngeal gargles, nasopharyngeal aspirates, serum, blood and in environmental samples. The use of PCR will enable the study of the epidemiology of P. carinii infection by detecting the organism in environmental samples, permitting molecular typing and thereby the study of the transmission of the organism. Furthermore PCR will facilitate studies on the response to therapy, studies monitoring for the emergence of drug resistant strains of P. carinii and in the diagnosis of P. carinii pneumonia in noninvasive specimens, in patients unable to undergo more invasive diagnostic procedures.
Lundgren
B
B
Department of Clinical Microbiology, Statens Serum Institut, Copenhagen, Denmark.
Wakefield
A E
AE
D004783:Environmental Microbiology; D006801:Humans; D011010:Pneumocystis / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*