Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient?
Four human colon cancer cell lines, HCT-8, HRT-18, DLD-1, and HCT-15, with an epithelioid morphotype reproducibly formed alpha-catenin-deficient round cells. Using DNA fingerprinting, we found that these four cell lines have an identical genetic background. Our finding strongly suggests a genetic background for the reproducible loss of alpha-catenin and the ensuing acquisition of invasiveness in all four cell lines.
Vermeulen
S J
SJ
Department of Radiotherapy and Nuclear Medicine, University Hospital Gent, Belgium.
Chen
T R
TR
Speleman
F
F
Nollet
F
F
Van Roy
F M
FM
Mareel
M M
MM
D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics*; D016172:DNA Fingerprinting; D004273:DNA, Neoplasm / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D007621:Karyotyping; D009363:Neoplasm Proteins / Q000032:analysis / Q000235:genetics*; D015534:Trans-Activators; D014407:Tumor Cells, Cultured / Q000473:pathology; D051176:beta Catenin
C495270:CTNNB1 protein, human; D003598:Cytoskeletal Proteins; D004273:DNA, Neoplasm; D005819:Genetic Markers; D009363:Neoplasm Proteins; D015534:Trans-Activators; D051176:beta Catenin