Chick skeletal muscle proteolysis in vitro increased by corticosterone.
Six-day-old chick skeletal muscle (extensor digitorum longus) was incubated in the presence of corticosterone (CTC; 0, 3, 30, and 300 ng/ml) for 2 h at 37 degrees C. Tyrosine and N (tau)-methylhistidine releases, as indices of total muscle and myofibrillar proteolysis, were increased by CTC but with different dose responses, indicating an independent regulation of myofibrillar and non-myofibrillar protein degradation.
Ohtsuka
A
A
Faculty of Agriculture, Department of Biochemical Science and Technology, Kagoshima University, Japan. ohtsuka@chem.agri.kagoshima-u.ac.jp
Tanaka
H
H
Hizaki
K
K
Hayashi
K
K
D000818:Animals; D002645:Chickens; D003345:Corticosterone / Q000378:metabolism* / Q000494:pharmacology; D010450:Endopeptidases / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008762:Methylhistidines / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D008762:Methylhistidines; D014443:Tyrosine; D010450:Endopeptidases; D003345:Corticosterone