Changes in the activities of mitochondrial enzymes in the progress of tumorigenesis of bladder cancer.
To investigate changes in the activities of mitochondrial enzymes in the progress of tumorigenesis of bladder cancer, N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) carcinogen was administered orally to male Sprague-Dawley rats for eight weeks. The urinary bladders were harvested periodically for the evaluation of tumorigenesis. The activities of NADH cytochrome c reductase (NCCR), succinate cytochrone c reductase (SCCR) and cytochrome c oxidase (CCO) were measured. The NCCR and SCCR activities elevated significantly by the exposure of BBN and decreased rapidly when BBN was withdrawn. However, the CCO activity increased and reached plateau at 18 weeks in spite of the discontinuance of BBN. The results indicated that the NCCR, SCCR and CCO activities were significantly elevated in the tumorigenesis. However, the CCO enzyme may be more related to the progress of tumorigenesis of bladder cancer.
Huang
C N
CN
Department of Urology, Kaohsiung Medical College, Taiwan.
Tsai
J L
JL
Chen
M T
MT
Wu
W J
WJ
Kuo
K W
KW
Huang
C H
CH
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002273:Carcinogens; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D002471:Cell Transformation, Neoplastic; D018450:Disease Progression; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D009245:NADH Dehydrogenase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism*; D001743:Urinary Bladder / Q000201:enzymology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000473:pathology
D002273:Carcinogens; D002085:Butylhydroxybutylnitrosamine; D013384:Succinate Cytochrome c Oxidoreductase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV