title: Dental plaque: an unlikely reservoir of Helicobacter pylori.
abstract: OBJECTIVE
  Dental plaque has been suggested as a reservoir for Helicobacter pylori, though data in this regard are conflicting. We evaluated the prevalence of H. pylori DNA in dental plaque using polymerase chain reaction (PCR).
  
  
  METHODS
  Antral H. pylori status of 156 patients with acid-peptic disease (APD) was studied by rapid urease test (RUT), histology and culture. Dental plaque obtained from these 156 patients and 92 healthy volunteers was evaluated for the presence of H. pylori using RUT, culture and PCR.
  
  
  RESULTS
  H. pylori was present in 133 antral biopsy samples by RUT and/or histology. The dental plaque of 37 patients with APD and 21 healthy volunteers tested positive by RUT. H. pylori was not isolated by culture from any of the dental plaques. PCR gave a significant amplification product in 11 of 248 (4.4%) dental plaque samples, 7 from patients with APD and 4 from normal healthy volunteers.
  
  
  CONCLUSIONS
  The frequency of H. pylori in the dental plaque is low, and this is unlikely to be a prominent site of infection with H. pylori.
authors.affiliation: Department of Microbiology, B Y L Nair Ch Hospital, Mumbai.
authors.forename: A H
authors.initials: AH
authors.lastname: Kamat
authors.forename: P R
authors.initials: PR
authors.lastname: Mehta
authors.forename: A A
authors.initials: AA
authors.lastname: Natu
authors.forename: A Y
authors.initials: AY
authors.lastname: Phadke
authors.forename: I M
authors.initials: IM
authors.lastname: Vora
authors.forename: P D
authors.initials: PD
authors.lastname: Desai
authors.forename: G V
authors.initials: GV
authors.lastname: Koppikar
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D017931:DNA Primers; D003773:Dental Plaque / Q000382:microbiology*; D004197:Disease Reservoirs; D004415:Dyspepsia / Q000382:microbiology*; D005260:Female; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D015995:Prevalence
chemical_list: D017931:DNA Primers