Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae PMR1 gene.
A gene homologous to Saccharomyces cerevisiae PMR1 has been cloned in the methylotrophic yeast Hansenula polymorpha. The partial DNA fragment of the H. polymorpha homologue was initially obtained by a polymerase chain reaction and used to isolate the entire gene which encodes a protein of 918 amino acids. The putative gene product contains all ten of the conserved regions observed in P-type ATPase. The cloned gene product exhibits 60.3% amino acid identity to the S. cerevisiae PMR1 gene product and complemented the growth defect of a S. cerevisiae pmr1 null mutant in the EGTA-containing medium. The results demonstrate that the H. polymorpha gene encodes the functional homologue of the S. cerevesiae PMR1 gene product, a P-type Ca(2+)-ATPase.
Kang
H A
HA
Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon, Korea.
Kim
J Y
JY
Ko
S M
SM
Park
C S
CS
Ryu
D D
DD
Sohn
J H
JH
Choi
E S
ES
Rhee
S K
SK
D018528:ATP-Binding Cassette Transporters / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D000252:Calcium-Transporting ATPases; D003001:Cloning, Molecular; D015342:DNA Probes / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry; D005816:Genetic Complementation Test; D018832:Molecular Chaperones; D008969:Molecular Sequence Data; D010843:Pichia / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D015342:DNA Probes; D004271:DNA, Fungal; D018832:Molecular Chaperones; C101767:SSC1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D000252:Calcium-Transporting ATPases