Effect of ethanol on the metabolism of trichloroethylene in the perfused rat liver.
The effect of 2 mM ethanol, a concentration indicative of daily alcohol consumption, was investigated on trichloroethylene (TRI) metabolism in perfused Wistar rat liver. The study consisted of two parts: The first part studied TRI administration with or without ethanol. In the second study chloral hydrate (CH), an intermediate in TRI metabolism, was administered in the absence or presence of ethanol to phenobarbital (PB) treated or non-PB-treated rats. The concentrations of the metabolites, total trichloroethanol (TCE), and trichloroacetic acid (TCA) were measured by gas chromatography and intracellular reduced pyridine nucleotides by surface fluorometry. In the first study, ethanol infusion significantly increased the TCE/TCA ratio, TCE production rate, and percentage of reduced pyridine nucleotides, and decreased TCA production rate without an associated change in the sum of TCE and TCA formation rates. In the second study, ethanol infusion in the absence or presence of PB produced similar significant increases in the TCE/TCA ratio, TCE production rate, and percentage of reduced pyridine nucleotides, accompanied by a decrease in TCA formation. The observed shift in TRI metabolism in the presence of ethanol, from oxidation to TCA to reduction to TCE, suggests that alcohol exerts alterations in hepatic intracellular oxidation-reduction (redox) states.
Watanabe
M
M
Department of Public Health and Environmental Science, School of Medicine, Tokyo Medical and Dental University, Japan.
Takano
T
T
Nakamura
K
K
D000818:Animals; D001711:Biotransformation; D002697:Chloral Hydrate / Q000494:pharmacology; D004334:Drug Administration Schedule; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D010084:Oxidation-Reduction / Q000187:drug effects; D010477:Perfusion / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D014241:Trichloroethylene / Q000493:pharmacokinetics*
D014241:Trichloroethylene; D000431:Ethanol; D002697:Chloral Hydrate