Preparative separation of alkaloids from the root of Sophora flavescens Ait by pH-zone-refining counter-current chromatography.
pH-Zone-refining counter-current chromatography was applied to the separation of alkaloids from a crude extract of the root of Sophora flavescens Ait using a multilayer coil planet centrifuge. After methyl tert.-butyl ether and water were equilibrated, triethylamine (10 mM) was added to the organic phase as a retainer and hydrochloric acid (5-10 mM) to the aqueous phase as an eluter. The separation was performed by eluting the aqueous phase while the organic phase was used as the stationary phase. From 1.0 g of the crude extract, sophocarpine (170 mg) and matrine (600 mg) were separated within 4.5 h at high purity of over 98%.
Yang
F
F
Beijing Institute of New Technology Application, China.
Quan
J
J
Zhang
T
T
Ito
Y
Y
D000470:Alkaloids / Q000302:isolation & purification*; D002845:Chromatography; D002855:Chromatography, Thin Layer; D003377:Countercurrent Distribution; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D018517:Plant Roots / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D012997:Solvents
D000470:Alkaloids; D007202:Indicators and Reagents; D012997:Solvents