A novel expression cloning method to isolate mammalian transcription factors in Schizosaccharomyces pombe.
We describe a novel expression cloning strategy in the fission yeast for the isolation of mammalian transcription factors using a mammalian promoter as target. This strategy is possible because of the conservation between mammalian cells and Schizosaccharomyces pombe of the mechanism that leads to the selection of the transcription start site. It also opens new perspectives to investigate the transcriptional regulation of genes for which detailed promoter analysis is difficult.
Remacle
J E
JE
Department of Cell Growth, Differentiation and Development, Flanders Interuniversity Institute for Biotechnology, University of Leuven, Campus Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium. jre@sgi.celgen.kuleuven.ac.be
Brys
R
R
Pype
S
S
Nelles
L
L
Huylebroeck
D
D
D002478:Cells, Cultured; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D011401:Promoter Regions, Genetic; D012568:Schizosaccharomyces / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics* / Q000302:isolation & purification*; D014158:Transcription, Genetic; D014162:Transfection; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
D018076:DNA, Complementary; D014157:Transcription Factors; D019010:Vascular Cell Adhesion Molecule-1