Platelet-derived growth factor (PDGF)-BB stimulates osteoclastic bone resorption directly: the role of receptor beta.
The growth-promoting activity of PDGF-BB was studied on the adult osteoclasts in the present study. The PDGF receptor beta was detected on the osteoclast membrane through immunohistochemistry (LSAB method) and immunomicroscopy. The PDGF-BB was exerted on the osteoclasts that adhered to the bone slice at concentrations of 0, 10, 20, 30, and 40 ng/ml. The volume of Howship's lacuna augmented significantly and the number of resorption pits also increased with its dose (p < 0.01). The activity of both total acid phosphatase (ACP) and tartrate-resistant acid phosphatase (TRAP) increased significantly. These results suggest that PDGF-BB promotes adult osteoclastic bone resorption directly through PDGF receptor beta and is believed to play important roles in the bone healing process and reconstruction.
Zhang
Z
Z
Nan Fang Hospital, First Military Medical University, Guangzhou, (510515), People's Republic of China.
Chen
J
J
Jin
D
D
D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000818:Animals; D000077214:Becaplermin; D001862:Bone Resorption / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D001842:Bone and Bones; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D016253:Microscopy, Immunoelectron; D010010:Osteoclasts / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010982:Platelet-Derived Growth Factor / Q000378:metabolism / Q000502:physiology*; D020574:Proto-Oncogene Proteins c-sis; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor / Q000502:physiology*
D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D000077214:Becaplermin; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor; D000135:Acid Phosphatase