Effect of sodium glycyrrhetinate on neonatal rat myocardial cells.
OBJECTIVE
To investigate the effect of sodium glycyrrhetinate (SG) on neonatal rat myocardial cells.


METHODS
The neonatal rat myocardial cells were cultured in vitro. Radioimmunoassay and fluorimetry were used to determine cAMP and [Ca2+], respectively.


RESULTS
The beating rate of myocardial cells was depressed by SG 0.4 mmol.L-1 at 5, 10, and 15 min, from 73 +/- 9 min-1 to 62 +/- 5, 59 +/- 7, and 56 +/- 6 min-1, respectively. SG 0.1 and 0.2 mmol.L-1 showed above similar results at 15 min and 10, 15 min, respectively. When the myocardial cells were incubated with SG 0.2 and 0.4 mmol.L-1 at 37 degrees C for 10 and 15 min, the concentration of cAMP and- [Ca2+] were reduced. cAMP contents in SG 0.2 mmol.L-1 treated group at 10 and 15 min were lower than control group (1.09 +/- 0.18 vs 1.65 +/- 0.48 pmol per vial, P < 0.05; 1.12 +/- 0.35 vs 1.72 +/- 0.49 pmol per vial, P < 0.01), and so was [Ca2+] (30 +/- 4 nmol.L-1 vs 41 +/- 6 nmol.L-1, P < 0.05 and 28 +/- 6 nmol.L-1 vs 38 +/- 7 nmol.L-1, P < 0.01). SG 0.1 and 0.2 nmol.L-1 increased the pO2 change rate (87% +/- 5%, 75% +/- 4% vs 54% +/- 3%, P < 0.01) in suspension fluid of myocardial cells, but SG 0.4 mmol.L-1 decreased it (31% +/- 2% vs 54% +/- 3%, P < 0.01).


CONCLUSIONS
SG protects myocardium or treats ischemic cardiac disease.
Zhu
X W
XW
Department of Pharmacology, Lanzhou Medical College, China.
Meng
F M
FM
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism*; D002316:Cardiotonic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D019695:Glycyrrhizic Acid / Q000494:pharmacology*; D006339:Heart Rate / Q000187:drug effects; D006352:Heart Ventricles; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D002316:Cardiotonic Agents; D019695:Glycyrrhizic Acid; D000242:Cyclic AMP; D002118:Calcium