Mass spectrometric survey of interganglionically transported peptides in Aplysia.
The major ganglionic connectives in Aplysia are assayed to determine putative neuropeptides. Matrix-assisted laser desorption/ionization mass spectrometry allows direct measurement of peptides in a nerve. Many previously characterized peptides are observed, including APGWamide, buccalins, small cardioactive peptides, and egg-laying hormone. Several unreported peptides are detected in specific nerves, suggesting they may have important physiological roles. Furthermore, novel processing products of the L5-67 precursor peptide and the APGWamide/cerebral peptide 1 prohormone are strongly suggested, and their interganglionic transport demonstrated.
Li
L
L
Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Moroz
T P
TP
Garden
R W
RW
Floyd
P D
PD
Weiss
K R
KR
Sweedler
J V
JV
D000818:Animals; D001048:Aplysia / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D001693:Biological Transport, Active / Q000502:physiology; D002490:Central Nervous System / Q000737:chemistry; D017952:Ganglia, Invertebrate / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D009479:Neuropeptides / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
C094043:LUQIN; D009479:Neuropeptides; C103535:cerebral peptide 1