Increased appetite augments hypothalamic NPY Y1 receptor gene expression: effects of anorexigenic ciliary neurotropic factor.
Neuropeptide Y (NPY) is a potent endogenous appetite transducer but the NPY receptor subtype mediating the induction of appetite is unknown. Evaluation of hypothalamic NPY Y1 and Y5 receptor mRNA by RNase protection assay showed that appetite evoked either by fasting or food-restriction increased expression of Y1 mRNA. Suppression of appetite by the cytokine, ciliary neurotropic factor, blocked this increase in Y1 gene expression. In contrast Y5 mRNA levels were unchanged by these treatments. These findings suggest that NPY-induced stimulation of appetite requires signal transmission though Y1 receptor subtype in the hypothalamus.
Xu
B
B
Department of Neuroscience, University of Florida College of Medicine, Gainesville 32610-0244, USA.
Kalra
P S
PS
Moldawer
L L
LL
Kalra
S P
SP
D000818:Animals; D000855:Anorexia / Q000139:chemically induced / Q000235:genetics / Q000503:physiopathology; D001066:Appetite / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D020934:Ciliary Neurotrophic Factor; D015536:Down-Regulation / Q000187:drug effects; D005215:Fasting / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects / Q000502:physiology; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000235:genetics* / Q000502:physiology
D020934:Ciliary Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C097212:neuropeptide Y-Y1 receptor; C100568:neuropeptide Y5 receptor