title: Overexpression of cation-dependent mannose 6-phosphate receptor prevents cell death induced by serum deprivation in PC12 cells.
abstract: PC12 cells express well cation-independent mannose 6-phosphate receptors (CI-MPR), but not cation-dependent (CD)-MPR as much. To examine CD-MPR dependency of transport of cathepsins B and D to lysosomes in PC12 cells, we prepared the cells overexpressing CD-MPR. Immunoreactivity for cathepsin B became more distinct and larger in size in the transfected cells than in wild-type cells. No difference in the distribution of cathepsin D was seen between these two cells. The viability of the cells following serum deprivation was significantly higher in the transfected cells than in wild-type cells. This increased viability of the transfected cells was blocked by CA074, a specific inhibitor of cathepsin B, while pepstatin A suppressed the action of CA074. The results suggest that CD-MPR preferentially transport cathepsin B in PC12 cells, and cathepsins B and D participate in the regulation of PC12 cell apoptosis.
authors.affiliation: Department of Conservative Dentistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Kanamori
authors.forename: S
authors.initials: S
authors.lastname: Waguri
authors.forename: M
authors.initials: M
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Isahara
authors.forename: Y
authors.initials: Y
authors.lastname: Ohsawa
authors.forename: A
authors.initials: A
authors.lastname: Konishi
authors.forename: S
authors.initials: S
authors.lastname: Kametaka
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: S
authors.initials: S
authors.lastname: Ebisu
authors.forename: E
authors.initials: E
authors.lastname: Kominami
authors.forename: Y
authors.initials: Y
authors.lastname: Uchiyama
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002401:Cathepsin B / Q000032:analysis; D002402:Cathepsin D / Q000032:analysis; D002412:Cations / Q000378:metabolism*; D002470:Cell Survival; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells / Q000378:metabolism*; D051381:Rats; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis*
chemical_list: D002412:Cations; D016895:Culture Media, Serum-Free; D017527:Receptor, IGF Type 2; D002401:Cathepsin B; D002402:Cathepsin D