Increased recovery of viable spermatozoa through oscillating centrifugation.
OBJECTIVE
To compare the number and quality of spermatozoa recovered following the standard centrifugation method with those recovered following oscillation of the relative centrifugal field during the centrifugation method.


METHODS
Prospectively controlled in vitro study.


METHODS
Private andrology laboratory.


METHODS
Seventeen healthy sperm donors.


METHODS
Equal halves of the same semen sample were evaluated following the standard centrifugation or following oscillation of the relative centrifugal field during centrifugation.


METHODS
Number of sperm, sperm motility, progressive sperm motility at 0 hours and after 3-hour of incubation at 5 degrees C in TES and Tris (buffer) (TEST)-yolk.


RESULTS
The number of sperm, sperm motility, progressive sperm motility at 0 hours and after 3-hour of incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following oscillating centrifugation compared with standard centrifugation.


CONCLUSIONS
Oscillating the relative centrifugal field during centrifugation yields higher number of viable spermatozoa.
Ozornek
M H
MH
Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.
Biefeld
P
P
Jeyendran
R S
RS
D002470:Cell Survival / Q000502:physiology; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D008297:Male; D009991:Oscillometry; D011446:Prospective Studies; D011785:Quality Assurance, Health Care; D012016:Reference Values; D013076:Sperm Count; D013081:Sperm Motility / Q000502:physiology*