Central role of hemoglobin degradation in mechanisms of action of 4-aminoquinolines, quinoline methanols, and phenanthrene methanols.
We have used a specific inhibitor of the malarial aspartic proteinase plasmepsin I and a nonspecific cysteine proteinase inhibitor to investigate the importance of hemoglobin degradation in the mechanism of action of chloroquine, amodiaquine, quinine, mefloquine (MQ), halofantrine, and primaquine. Both proteinase inhibitors antagonized the antiparasitic activity of all drugs tested with the exception of primaquine. An inhibitor of plasmepsin I, Ro40-4388, reduced the incorporation of radiolabelled chloroquine and quinine into malarial pigment by 95%, while causing a 70% reduction in the incorporation of radiolabelled MQ. Cysteine proteinase inhibitor E64 reduced the incorporation of chloroquine and quinine into malarial pigment by 60 and 40%, respectively. This study provides definitive support for the central role of hemoglobin degradation in the mechanism of action of the 4-aminoquinolines and the quinoline and phenanthrene methanol antimalarials.
Mungthin
M
M
Department of Pharmacology and Therapeutics, The University of Liverpool, Liverpool L69 3BX, United Kingdom.
Bray
P G
PG
Ridley
R G
RG
Ward
S A
SA
D000655:Amodiaquine / Q000378:metabolism / Q000494:pharmacology; D000962:Antimalarials / Q000494:pharmacology*; D002738:Chloroquine / Q000378:metabolism / Q000494:pharmacology; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D015767:Mefloquine / Q000378:metabolism / Q000494:pharmacology; D010616:Phenanthrenes / Q000378:metabolism / Q000494:pharmacology; D011319:Primaquine / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology
D000962:Antimalarials; D006454:Hemoglobins; D010616:Phenanthrenes; D011480:Protease Inhibitors; D000655:Amodiaquine; D002738:Chloroquine; D007930:Leucine; D011319:Primaquine; C023768:halofantrine; C024974:E 64; D015767:Mefloquine