Immunohistochemical detection of Dirofilaria immitis using a rabbit polyclonal anti-serum against adult somatic antigens and excretory-secretory products.
An immunohistochemical study on tissue sections of kidney, lung, liver and spleen from six dogs naturally infected by Dirofilaria immitis was carried out, using a rabbit polyclonal anti-serum against somatic antigen (SA) of adult worms, excretory-secretory products (ES) of adult worms and fraction C3 of complement (Dog C3). Positive immunoreactions were observed on circulating microfilariae when anti-SA and anti-ES were used, but no differences were noted between the two anti-sera. No antigen deposits were observed when the three anti-sera (anti-SA, anti-ES or anti-C3) were employed as primary antibody. In two cases of human filariosis (by Loa loa and Mansonella streptocerca) no positive immunoreactions were observed.
Molina
J M
JM
Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Spain.
Oros
J
J
Ferrer
O
O
Rodrigues
E
E
Gutierrez
A C
AC
Hernández
S
S
D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology*; D004183:Dirofilaria immitis / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D004283:Dog Diseases / Q000469:parasitology; D004285:Dogs / Q000469:parasitology; D005260:Female; D006801:Humans; D007106:Immune Sera; D007668:Kidney / Q000469:parasitology; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008297:Male; D008842:Microfilariae; D011817:Rabbits
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D007106:Immune Sera