Size separation of sodium dodecyl sulfate complexes of human plasma proteins by capillary electrophoresis employing linear polyacrylamide as a sieving polymer.
Electrophoretic conditions to separate sodium dodecyl sulfate (SDS) complexes of human plasma proteins according to their size differences, by capillary electrophoresis employing linear polyacrylamide as a sieving matrix (LPA-CE), have been examined. Using the optimized separation conditions, SDS complexes of human plasma proteins not treated with reducing agents were separated into about 40 peaks and shoulders within 60 min. The molecular mass values of major peaks in a separation pattern were estimated from a plot of molecular mass and migration time for standard proteins and some of the major plasma proteins have been identified on the pattern. The electrophoretic conditions were successfully applied for the analysis of proteins in immunoglobulin G (IgG) myeloma sera.
Manabe
T
T
Department of Chemistry, Faculty of Science, Ehime University, Matsuyama, Japan. manabet@dpc.ehime-u.ac.jp
Oota
H
H
Mukai
J
J
D000180:Acrylic Resins; D001798:Blood Proteins / Q000737:chemistry / Q000302:isolation & purification*; D002021:Buffers; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008970:Molecular Weight; D009194:Myeloma Proteins / Q000032:analysis; D010316:Particle Size; D011108:Polymers; D015203:Reproducibility of Results; D012967:Sodium Dodecyl Sulfate; D000099166:Polyacrylamides
D000180:Acrylic Resins; D001798:Blood Proteins; D002021:Buffers; D007074:Immunoglobulin G; D009194:Myeloma Proteins; D011108:Polymers; D012967:Sodium Dodecyl Sulfate; D000099166:Polyacrylamides