Serine protease detection in mixed lymphocyte cultures: a histochemical method for possible prediction of graft-versus-host disease.
OBJECTIVE
Graft-versus-host disease (GVHD) presents an important complication of allogeneic bone marrow transplantation. A method to predict GVHD might be the analysis of cytotoxic T lymphocyte precursors, but the technique requires the use of radioactive elements not suitable in all laboratories.


METHODS
Serine esterase (SE) activity was studied by a cytochemical method in donor-recipient mixed lymphocytes cultures (MLC). Twelve patients, affected by acute or chronic leukemia, and 20 donors were studied. MLC incubated with and without growth factors (IGF-1 or IL-2), were analyzed. The relationship between positivity of MLC and GVHD in transplanted patients was evaluated.


RESULTS
The data obtained showed that the percentage of SE positive cells was higher in MLC compared to negative control MLC. The highest percentage of positive cells was found in the MLC obtained from unrelated subjects.


CONCLUSIONS
These results show that serine protease expression in MLC may be a predictive marker of GVHD and could be used as an additional early test associated with cytotoxicity.
Maiocchi
M A
MA
Dipartimento di Biologia Animale, Università di Pavia, Italy.
Nano
R
R
Capelli
E
E
Bonfichi
M
M
Alessandrino
E P
EP
Bernasconi
P
P
D000328:Adult; D015415:Biomarkers; D004796:Clinical Enzyme Tests; D003594:Cytoplasmic Granules / Q000201:enzymology; D004950:Esterases / Q000032:analysis*; D006086:Graft vs Host Disease / Q000517:prevention & control*; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000201:enzymology*; D008875:Middle Aged; D012697:Serine Endopeptidases / Q000032:analysis*
D015415:Biomarkers; D004950:Esterases; C022635:serine esterase; D012697:Serine Endopeptidases