title: Inhibitory effects of Serenoa repens on the kinetic of pig prostatic microsomal 5alpha-reductase activity.
abstract: The pathogenesis of benign prostatic hyperplasia is linked to the accumulation of dihydrotestosterone (DHT), the active form of testosterone (T), in prostatic tissue. We have defined characteristics of 5alpha-reductase enzyme which catalyzes the conversion of T into DHT in prostatic microsomes of growing pigs. Peaks for the 5alpha-reductase activity were found at pH 5.5 and 8.0, which indicates the presence of both type 1 and type 2 isozymes. Kinetic parameters of porcine 5alpha-reductase in the presence of Serenoa repens extracts revealed uncompetitive, noncompetitive, and mixed types of inhibitions. Our results show the inhibitory action of S. repens on prostate porcine microsomal 5alpha-reductase activity.
authors.affiliation: Agriculture and Agri-Food Canada, Dairy and Swine Research and Development Centre, Lennoxville, Quebec. Palinmf@EM.AGR.CA
authors.forename: M F
authors.initials: MF
authors.lastname: Palin
authors.forename: M
authors.initials: M
authors.lastname: Faguy
authors.forename: J G
authors.initials: JG
authors.lastname: LeHoux
authors.forename: G
authors.initials: G
authors.lastname: Pelletier
mesh_terms: D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D058891:5-alpha Reductase Inhibitors; D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D010936:Plant Extracts / Q000494:pharmacology*; D011467:Prostate / Q000201:enzymology* / Q000648:ultrastructure; D013552:Swine
chemical_list: D058891:5-alpha Reductase Inhibitors; D004791:Enzyme Inhibitors; D010936:Plant Extracts; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase