[Detection of poliovirus by digoxigenin-labeled cDNA probe prepared by PCR technique].
A study including reverse transcribed cDNAs of Sabin I, II, III virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid, more sensitive and more specific for poliovirus typing.
Liu
M T
MT
Guangxi Health and Anti-epidemic Center, Nanning.
Wang
S S
SS
Zhu
T F
TF
D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics; D004076:Digoxigenin; D006801:Humans; D017955:Poliovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D018076:DNA, Complementary; D004279:DNA, Viral; D004076:Digoxigenin