title: Patterns of cytokine expression in AIDS-related non-Hodgkin's lymphoma.
abstract: The pathogenesis of AIDS-related non-Hodgkin's lymphomas (AIDS-NHL) involves accumulation of genetic lesions, stimulation and selection by antigen, as well as infection by viruses. Deregulation of cytokine loops has also been proposed to contribute to AIDS-NHL development, although data are available only for a limited number of cytokines. In this study we have utilized a panel of AIDS-NHL cell lines to investigate in detail the pattern of tumour expression and production of a wide spectrum of cytokines. The cytokines investigated included interleukin (IL)-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-13, TNF alpha, TNF beta, IFN gamma, TGF beta2, G-CSF, GM-CSF and SCF. The AIDS-NHL cell lines utilized were representative of both AIDS-related Burkitt lymphoma (AIDS-BL) and AIDS-related body cavity-based lymphoma (AIDS-BCBL). Overall, AIDS-NHL were found to produce IL-6, IL-10 and TNF beta, although with different patterns depending upon the biological features of the tumour. Production of high levels of IL10 preferentially associated with Epstein-Barr virus (EBV) positive AIDS-BL and AIDS-BCBL, although lower levels of the cytokine were also detectable among EBV-negative AIDS-BL. Production of IL-6 was restricted to EBV-positive AIDS-BL and AIDS-BCBL, whereas it was absent among EBV-negative AIDS-BL. Production of TNF beta clustered with AIDS-BL, whereas this was absent among AIDS-BCBL. These results define that the pattern of cytokine expression of AIDS-NHL depends upon the biological features of the tumour and may have implications for the pathogenesis of these disorders.
authors.affiliation: Dipartimento di Scienze Cliniche e Biologiche, Ospedale San Luigi, Università di Torino, Orbassano-Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Pastore
authors.forename: G
authors.initials: G
authors.lastname: Gaidano
authors.forename: P
authors.initials: P
authors.lastname: Ghia
authors.forename: L
authors.initials: L
authors.lastname: Fassone
authors.forename: A M
authors.initials: AM
authors.lastname: Cilia
authors.forename: A
authors.initials: A
authors.lastname: Gloghini
authors.forename: D
authors.initials: D
authors.lastname: Capello
authors.forename: D
authors.initials: D
authors.lastname: Buonaiuto
authors.forename: S
authors.initials: S
authors.lastname: Gonella
authors.forename: S
authors.initials: S
authors.lastname: Roncella
authors.forename: A
authors.initials: A
authors.lastname: Carbone
authors.forename: G
authors.initials: G
authors.lastname: Saglio
mesh_terms: D016207:Cytokines / Q000378:metabolism*; D019288:Herpesvirus 8, Human / Q000302:isolation & purification; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D016483:Lymphoma, AIDS-Related / Q000378:metabolism* / Q000821:virology; D012313:RNA / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D007378:Interleukins; D014409:Tumor Necrosis Factor-alpha; D012313:RNA