An experimental model of diabetes and cancer in rats.
The aim of this study was to develop an experimental model for the study of cancer associated with diabetes. For diabetes induction, Sprague-Dawley rats were given streptozotocin (STZ, 90 mg/kg body weight (BW), by intraperitoneal injection on the second day of life. For mammary tumour induction, rats were injected with 50 mg/kg BW of N-nitroso-N-methylurea (NMU) at 50, 80 and 110 days old. The neoplastic process and the effect of tamoxifen treatment was examined in non-diabetic and diabetic rats. The latency period, NMU-induced tumour incidence and the number of tumours per rat in diabetic rats versus controls were 117 +/- 7 days versus 79 +/- 9 days (P < 0.001); 93% versus 95% (NS); and 5.2 +/- 1.6 versus 2.7 +/- 0.5 (P < 0.02). A more benign histological pattern for tumours in diabetic animals was observed. Mammary tumours in diabetic rats grew more slowly than in controls. Tamoxifen (1 mg/kg/day) treated diabetic rats showed tumour regression in 67% of NMU-induced mammary tumours versus 53% in controls (NS). Our results show that tumour progression seems to be affected by diabetes in this experimental model. We suggest this is the result of changes to insulin-like growth factors and their receptors, which occur in diabetics, and our future research will examine this hypothesis.
Cocca
C
C
Laboratorio de Radiosótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Martin
G
G
Rivera
E
E
Davio
C
C
Cricco
G
G
Lemos
B
B
Fitzsimons
C
C
Gutierrez
A
A
Levin
E
E
Levin
R
R
Croci
M
M
Bergoc
R M
RM
D000818:Animals; D000900:Anti-Bacterial Agents; D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D002273:Carcinogens / Q000633:toxicity; D002455:Cell Division; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000473:pathology; D005260:Female; D007328:Insulin / Q000097:blood; D008325:Mammary Neoplasms, Experimental / Q000097:blood / Q000209:etiology* / Q000473:pathology; D008770:Methylnitrosourea / Q000633:toxicity; D051381:Rats; D013311:Streptozocin; D013629:Tamoxifen / Q000627:therapeutic use*
D000900:Anti-Bacterial Agents; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D007328:Insulin; D013629:Tamoxifen; D013311:Streptozocin; D008770:Methylnitrosourea