[The application of the hybridization in colonies technic for the identification of toxigenic Vibrio cholerae 01].
By means of the polymerase chain reaction (PCR) it was obtained a probe for the gen that codifies the subunit B of cholerae toxin (CTxB), which carried a Vibrio cholerae 01 reference strain. The checking of the amplified product was performed by using the hybridization techniques in colonies. This product hybridized with the gen that codifies for the subunit B of cholerae toxin isolated from Peru and Ecuador, representing the present epidemics in Latin America, but it did not so with the phylogenetically related strains.
Bravo Fariñas
L
L
Instituto de Medicina Tropical Pedro Kourí.
Monté Boada
R J
RJ
Ramírez Alvarez
M
M
Maestre Mesa
J L
JL
Suárez Moreno
O
O
Morales Grillo
J
J
D001431:Bacteriological Techniques; D002772:Cholera Toxin / Q000235:genetics*; D004484:Ecuador; D005798:Genes, Bacterial; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007194:India; D010568:Peru; D016133:Polymerase Chain Reaction; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification
D002772:Cholera Toxin