title: [Effect of acute hypoxia on ATP-sensitive potassium current in ventricular myocytes of guinea-pig].
abstract: The role of the ATP-sensitive potassium channel in arrhythmogenesis is not clearly understood. Cellular K+ loss and accumulation of [K+]o may contribute to genesis of malignant ventricular arrhythmia during myocardial ischemia. In the present study, acute hypoxia simply caused a partial decrease in K+ efflux at normal [K+]o, which was not sensitive to glibenclamide (5 x 10(-3) mmol/L). However, at a higher concentration of [K+]o (10.8 mmol/L), the outward K+ current increased dramatically after 10 min hypoxia, which was accompanied with an irreversible hypercontracture and eventual death of the cell. The I-V relation was linear with increasing repolarization, which was blocked by glibenclamide, an antagonist of ATP-sensitive potassium channel. The results suggest that the increased K+ current is ATP-sensitive and is facilitated by accumulation of the [K+]o.
authors.affiliation: Shanghai Institute of Physiology, Chinese Academy of Sciences.
authors.forename: S J
authors.initials: SJ
authors.lastname: Pan
authors.forename: Z N
authors.initials: ZN
authors.lastname: Zhou
mesh_terms: D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors / Q000502:physiology; D000818:Animals; D001693:Biological Transport, Active; D015687:Cell Hypoxia; D005260:Female; D005905:Glyburide / Q000494:pharmacology; D006168:Guinea Pigs; D007004:Hypoglycemic Agents / Q000494:pharmacology; D008297:Male; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism*; D015221:Potassium Channels / Q000378:metabolism*
chemical_list: D007004:Hypoglycemic Agents; D015221:Potassium Channels; D000255:Adenosine Triphosphate; D011188:Potassium; D005905:Glyburide