A functional myo-inositol catabolism pathway is essential for rhizopine utilization by Sinorhizobium meliloti.
Rhizopine (L-3-O-methyl-scyllo-inosamine) is a symbiosis-specific compound found in alfalfa nodules induced by specific Sinorhizobium meliloti strains. It has been postulated that rhizobial strains able to synthesize and catabolize rhizopine gain a competitive advantage in the rhizosphere. The pathway of rhizopine degradation is analysed here. Since rhizopine is an inositol derivative, it was tested whether inositol catabolism is involved in rhizopine utilization. A genetic locus required for the catabolism of inositol as sole carbon source was cloned from S. meliloti. This locus was delimited by transposon Tn5 mutagenesis and its DNA sequence was determined. Based on DNA similarity studies and enzyme assays, this genetic region was shown to encode an S. meliloti myo-inositol dehydrogenase. Strains that harboured a mutation in the myo-inositol dehydrogenase gene (idhA) did not display myo-inositol dehydrogenase activity, were unable to utilize myo-inositol as sole carbon/energy source, and were unable to catabolize rhizopine. Thus, myo-inositol dehydrogenase activity is essential for rhizopine utilization in S. meliloti.
Galbraith
Mark P
MP
Department of Biological Sciences, Western, Michigan UniversityKalamazoo, MI 49008USA.
Feng
Szi Fei
SF
Department of Biological Sciences, Western, Michigan UniversityKalamazoo, MI 49008USA.
Borneman
James
J
Department of Agronomy and Center for the Study of Nitrogen Fixation, University of Wisconsin- MadisonMadison, WI 53706USA.
Triplett
Eric W
EW
Department of Agronomy and Center for the Study of Nitrogen Fixation, University of Wisconsin- MadisonMadison, WI 53706USA.
de Bruijn
Frans J
FJ
MSU-DOE Plant Research Laboratory, Department of Microbiology, NSF Center for Microbial Ecology, Michigan State UniversityEast Lansing, MI 48824USA.
Rossbachl
Silvia
S
Department of Biological Sciences, Western, Michigan UniversityKalamazoo, MI 49008USA.
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003227:Conjugation, Genetic; D005816:Genetic Complementation Test; D007294:Inositol / Q000031:analogs & derivatives* / Q000378:metabolism*; D000455:Medicago sativa / Q000382:microbiology; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009243:NAD / Q000378:metabolism; D009586:Nitrogen Fixation / Q000235:genetics; D016366:Open Reading Frames / Q000235:genetics; D010641:Phenotype; D015183:Restriction Mapping; D012230:Rhizobiaceae / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013401:Sugar Alcohol Dehydrogenases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D009243:NAD; C082577:rhizopine; D007294:Inositol; D013401:Sugar Alcohol Dehydrogenases; C021448:myo-inositol 2-dehydrogenase