title: Homing of mononuclear cells from iliac lymph nodes to the genital and rectal mucosa in non-human primates.
abstract: The route of immunization may affect the type of immunity that is induced. The objectives of this investigation were to establish in the non-human primate if the internal iliac lymph nodes (LN) function as an inductive site of immunity from which mononuclear cells home to the rectal and cervico-vaginal mucosa. Rhesus macaques were immunized with simian immunodeficiency virus (SIV) core antigen p27 in the proximity of the iliac lymph nodes, and compared with the intramuscular (i.m.) (deltoid or gluteal), and axillary LN routes of immunization. The macaques were then challenged rectally or vaginally by a particulate SIVp27 antigen which was applied to the mucosal surface. The tracking dye PKH26 was injected near the immunizing LN or i.m. site and a week later the mucosal and lymphoid tissues were examined at autopsy. Preferential homing of PKH26-labeled cells from the internal iliac LN to the rectal and vaginal mucosa was demonstrated by flow cytometry after targeted iliac LN (TILN) but not after intramuscular (deltoid) or axillary LN immunization. Homing of the subsets of cells revealed that labeled CD4, CD8 and B cells, as well as monocytes were found in the rectum, colon, vagina or cervix. The results of this investigation shows that the route of immunization may affect regional mucosal immunity. Furthermore, the internal iliac LN may function as an inductive immunological site from which CD4, CD8 and B cells may home preferentially to the rectal, cervical and vaginal mucosa, as well as to the related regional but not the unrelated distal LN.
authors.affiliation: Department of Immunology, United Medical & Dental Schools of Guy's & St. Thomas' Hospitals, London, GB.
authors.forename: E A
authors.initials: EA
authors.lastname: Mitchell
authors.forename: L A
authors.initials: LA
authors.lastname: Bergmeier
authors.forename: C
authors.initials: C
authors.lastname: Doyle
authors.forename: R
authors.initials: R
authors.lastname: Brookes
authors.forename: L A
authors.initials: LA
authors.lastname: Hussain
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: T
authors.initials: T
authors.lastname: Lehner
mesh_terms: D000818:Animals; D002465:Cell Movement; D005260:Female; D005456:Fluorescent Dyes; D015683:Gene Products, gag / Q000276:immunology; D007085:Ilium / Q000276:immunology*; D018928:Immunity, Mucosal; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology*; D008214:Lymphocytes; D008253:Macaca mulatta; D009092:Mucous Membrane / Q000166:cytology / Q000276:immunology; D009930:Organic Chemicals; D011323:Primates; D012007:Rectum / Q000166:cytology / Q000276:immunology*; D014621:Vagina / Q000166:cytology / Q000276:immunology*
chemical_list: D005456:Fluorescent Dyes; C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D009930:Organic Chemicals; C070080:PKH 26