title: Growth temperature ranges of Borrelia burgdorferi sensu lato strains.
abstract: Three strains of Borrelia burgdorferi sensu lato, representing three human pathogenic genomospecies (B31, B. burgdorferi sensu stricto; BR14, B. garinii; BR75, B. afzelii) were grown in BSK-H medium at different temperatures and the spirochaetal cells were counted by dark-field microscopy after 0, 4, 8, 16 and 48 days. Approximate optimum (minimum-maximum) temperatures for the in-vitro growth were found to be 33 degrees C (22-39 degrees C) in strain B31, 35 degrees C (20-40 degrees C) in strain BR75 and 37 degrees C (20-41 degrees C) in strain BR14. Maximum, optimum and minimum growth temperatures seem to be important characteristics of B. burgdorferi s.l. strains, with relevance for the symptomatology, epidemiology and epizoology of Lyme borreliosis.
authors.affiliation: Institute of Landscape Ecology, Academy of Sciences, Brno, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Hubálek
authors.forename: J
authors.initials: J
authors.lastname: Halouzka
authors.forename: M
authors.initials: M
authors.lastname: Heroldová
mesh_terms: D000818:Animals; D001096:Arachnid Vectors / Q000382:microbiology; D001717:Birds / Q000382:microbiology; D001831:Body Temperature; D015748:Borrelia burgdorferi Group / Q000254:growth & development*; D006801:Humans; D007303:Insect Vectors / Q000382:microbiology; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000382:microbiology; D008322:Mammals / Q000382:microbiology; D005423:Siphonaptera / Q000382:microbiology; D012881:Skin Temperature; D013696:Temperature