Vitamin D3 inhibits proliferation and increases c-myc expression in fibroblasts from psoriatic patients.
Fibroblast cultures were established from the skin of normal and psoriatic subjects. The response to 1 alpha,25-dihydroxyvitamin D3 [1,25-(OH)2D3] of each kind of cells was assessed by measuring tritiated thymidine incorporation into DNA as an index of cell proliferation. We found that both types of cells responded with a similar dose- and time-dependent inhibition of thymidine incorporation. We also studied the response of the mRNA encoding the proto-oncogene c-myc, since its level is associated to the proliferative state in many cell types. Psoriatic fibroblasts contained higher basal amounts of c-myc RNA than control fibroblasts. Addition of 1,25-(OH)2D3 to the culture medium induced a time-dependent increase of c-myc RNA in psoriatic fibroblasts but not in controls. As a control, retinoic acid had no effect in any of the two cell types. It is concluded that in primary normal human fibroblasts, c-myc RNA levels are not correlated with the proliferative state, and that there is an altered expression of this proto-oncogene in psoriasis.
Casado
M
M
Department of Dermatology, Ciudad Sanitaria La Paz, Spain.
Martin
M
M
Muñoz
A
A
Bernal
J
J
D000328:Adult; D015152:Blotting, Northern; D002117:Calcitriol / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D015342:DNA Probes; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects*; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D008297:Male; D000090063:Proto-Oncogene Mas; D011565:Psoriasis / Q000378:metabolism / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology; D014284:Triiodothyronine / Q000494:pharmacology
D003470:Culture Media; D015342:DNA Probes; C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D014284:Triiodothyronine; D014212:Tretinoin; D004247:DNA; D002117:Calcitriol