title: Determination of hepatotoxic pyrrolizidine alkaloids by on-line high performance liquid chromatography mass spectrometry with an electrospray interface.
abstract: The present study describes the determination of two different types of hepatotoxic pyrrolizidine alkaloids (PAs) and also distinguishing the hepatotoxic PAs from non-toxic ones by both in-source collision-induced dissociation high performance liquid chromatography mass spectrometry (CID-HPLC/MS) and HPLC/MS/MS (CID in the collision cell), using electrospray ionization. The mass spectra provided molecular ions and characteristic fragment ions, which could be used readily for a rapid identification of different types of PAs. Applications of both in-source CID-HPLC/MS and HPLC/MS/MS analytical methods were successful for the determination of PAs in blood samples obtained from rats dosed with PAs and in the PA-containing plant. The results demonstrated that the developed HPLC/MS methods with two different CID techniques provided a very simple and rapid analysis for an unequivocal diagnosis of PA poisoning and for definitive identification of PAs in plants or herbal medicines.
authors.affiliation: Department of Pharmacology, Chinese University of Hong Kong, Shatin, SAR.
authors.forename: G
authors.initials: G
authors.lastname: Lin
authors.forename: K Y
authors.initials: KY
authors.lastname: Zhou
authors.forename: X G
authors.initials: XG
authors.lastname: Zhao
authors.forename: Z T
authors.initials: ZT
authors.lastname: Wang
authors.forename: P P
authors.initials: PP
authors.lastname: But
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D011763:Pyrrolizidine Alkaloids