Lack of correlation between repair of DNA interstrand cross-links and hypersensitivity of hamster cells towards mitomycin C and cisplatin.
The ability to repair DNA interstrand cross-links may be an important factor contributing to mitomycin C (MMC) and cisplatin cytotoxicities. We have assessed the repair of interstrand cross-links induced by MMC in two MMC-hypersensitive hamster cell mutants and their resistant parental cell line. Using a gene-specific repair assay, we found no evidence for repair of MMC cross-links in either parental or mutant cells, suggesting that persistence of DNA interstrand cross-links is not responsible for the differential toxicity of MMC towards hypersensitive cells. Repair of cisplatin-induced interstrand cross-links was efficient in resistant as well as in mutant cells. Therefore we concluded that a defect in excision repair of interstrand cross-links was not responsible for the cytotoxic effects of MMC and cisplatin in these hypersensitive mutants.
Larminat
F
F
Institut de Pharmacologie et de Biologie Structurale, UPR 9062, C.N.R.S., 205, Toulouse, France. flol@ipbs.fr
Cambois
G
G
Zdzienicka
M Z
MZ
Defais
M
M
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D003114:Colony-Forming Units Assay; D006224:Cricetinae; D003412:Cricetulus; D003432:Cross-Linking Reagents; D004260:DNA Repair; D016685:Mitomycin / Q000494:pharmacology*; D009154:Mutation
D003432:Cross-Linking Reagents; D016685:Mitomycin; D002945:Cisplatin