title: Regulation of the Escherichia coli sheA gene and characterization of its encoded hemolytic activity.
abstract: Escherichia coli K-12 carries the cryptic hemolysin gene sheA which is under the control of positive and negative transcriptional regulators. The objectives of the present study were to further analyze the regulation of the sheA gene in E. coli, to compare the sheA genes from E. coli K-12 and a pathogenic E. coli strain, and to characterize the SheA hemolytic activity. Northern blot analysis demonstrated that the transcriptional regulator SlyA activates the E. coli K-12 sheA gene. The main transcriptional start site of the sheA gene was 56 nucleotides upstream from the start codon as determined by primer extension analysis. The sheA genes from E. coli K-12 and a pathogenic E. coli strain were identical. SheA hemolytic activity was cell associated and Ca2+ independent.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln 68583-0905, USA.
authors.forename: S V
authors.initials: SV
authors.lastname: Fernández
authors.forename: J
authors.initials: J
authors.lastname: Xing
authors.forename: V
authors.initials: V
authors.lastname: Kapur
authors.forename: S J
authors.initials: SJ
authors.lastname: Libby
authors.forename: R G
authors.initials: RG
authors.lastname: Barletta
authors.forename: R A
authors.initials: RA
authors.lastname: Moxley
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D004927:Escherichia coli Infections / Q000662:veterinary; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism; D006461:Hemolysis; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012329:RNA, Bacterial / Q000032:analysis; D017422:Sequence Analysis, DNA; D013552:Swine; D014158:Transcription, Genetic
chemical_list: D029968:Escherichia coli Proteins; D006460:Hemolysin Proteins; D012329:RNA, Bacterial; C119944:hlyE protein, E coli; D002118:Calcium