Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis.
The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences. A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A. pleuropneumoniae serotype 2 seem to be clonally related.
Fussing
V
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Danish Veterinary Laboratory, Copenhagen, Denmark. vfu@ssi.dk
D000189:Actinobacillus Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D000818:Animals; D015373:Bacterial Typing Techniques; D002170:Canada / Q000453:epidemiology; D016000:Cluster Analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D016521:Electrophoresis, Gel, Pulsed-Field; D005060:Europe / Q000453:epidemiology; D020459:Genes, rRNA; D008168:Lung / Q000382:microbiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000382:microbiology*
D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D015252:Deoxyribonucleases, Type II Site-Specific