A novel method to immobilize bioactive substances on hydrophobic surfaces using a polymerizable cationic lipid.
We have successfully developed a novel method to stably immobilize bioactive substances that have anionic groups, such as heparin and succinylated collagen (SC), on hydrophobic surfaces through ionic complexation using a polymerizable cationic lipid, diallyl(dioleyl)ammonium bromide (DADOA). It is composed of a hydrophobic part consisting of long hydrocarbon chains and a hydrophilic head with double bonds which render it polymerizable. Analysis of the modification with DADOA and heparin suggested that the modification formed a thin layer, roughly 60 nm in thickness, as a result of the spontaneous deposition of DADOA and heparin dissolved in water, through the hydrophobic interaction between DADOA and the surface and the ionic complexation between DADOA and heparin. The heparin deposition and its rate of release in plasma were 1.5 microg/cm2 and 0.0017 U/cm2/min, respectively. Cytotoxicity test results showed that the polymerization of the deposited DADOA rendered the modified surface stable and noncytotoxic. Further, antithrombogenicity and cell attachability test results demonstrated that heparin and SC were effectively immobilized on hydrophobic surfaces through ionic complexation. This method has proved useful for the modification of the hydrophobic surfaces of medical devices because the modification process can be performed under aqueous conditions without the use of organic solvents which induce crazing/cracking of plastic casings.
Yamazaki
M
M
Department of Membrane and Biomedical Materials, Japan Research Center, W.R. Grace & Co. Connecticut, Atsugi.
Kobayashi
K
K
Nakai
T
T
Mikami
M
M
Yoshioka
H
H
Mori
Y
Y
Satoh
T
T
Kubota
S
S
D000498:Allyl Compounds / Q000737:chemistry*; D000818:Animals; D000925:Anticoagulants / Q000097:blood / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001965:Bromides / Q000737:chemistry*; D002412:Cations; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry; D004285:Dogs; D005347:Fibroblasts / Q000187:drug effects; D005404:Fixatives; D006493:Heparin / Q000097:blood / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008297:Male; D011108:Polymers / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds; D051381:Rats; D017208:Rats, Wistar; D013386:Succinates / Q000737:chemistry; D013499:Surface Properties; D013927:Thrombosis / Q000517:prevention & control
D000498:Allyl Compounds; D000925:Anticoagulants; D001672:Biocompatible Materials; D001965:Bromides; D002412:Cations; D002614:Chelating Agents; D020099:Coated Materials, Biocompatible; D005404:Fixatives; D008055:Lipids; D011108:Polymers; D000644:Quaternary Ammonium Compounds; D013386:Succinates; C116182:diallyl(dioleyl)ammonium bromide; D006493:Heparin; D003094:Collagen; C051470:ammonium bromide