title: Functional changes in THP-1 human monocytic cells after stimulation with lipopolysaccharide of oral microorganisms and granulocyte macrophage colony stimulating factor.
abstract: A human THP-1 monocyte cell line culture system has been utilized to observe the effect of granulocyte macrophage colony stimulating factor (GM-CSF) supplementation with lipopolysaccharide (LPS) of oral microorganisms to stimulate monocyte/macrophage functional activity. LPS of oral microorganisms, Fusobacterium nucleatum and Porphyromonas gingivalis was produced by phenol-water extraction and characterized. The phagocytosis assay was performed using F1TC labeled Saccharomyces yeast particles. Phagocytic functional activity was observed in 10-11% of resting THP-1 cells. Treatment of THP-1 cells with LPS of F. nucleatum or P. gingivalis increased the phagocytic activity of THP-1 cells 2-3 fold. GM-CSF significantly increased phagocytosis either alone or when supplemented with LPS of F. nucleatum or P. gingivalis. A chemotaxis assay was performed using a 48 well chemotaxis chamber. Chemotactic functional activity of THP-1 cells was increased 2-fold after 4 days of treatment with GM-CSF. Stimulation of THP-1 cells with LPS of F. nucleatum or P. gingivalis significantly reduced the chemotactic activity indicating the maturation towards a fixed macrophage. There were functional variations (chemotaxis and phagocytosis) in THP-1 cells in response to LPS of oral microorganisms following stimulation with GM-CSF.
authors.affiliation: Department of Oral Medicine, Dental School, University of Maryland, Baltimore 21201, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Baqui
authors.forename: T F
authors.initials: TF
authors.lastname: Meiller
authors.forename: B F
authors.initials: BF
authors.lastname: Turng
authors.forename: J I
authors.initials: JI
authors.lastname: Kelley
authors.forename: W A
authors.initials: WA
authors.lastname: Falkler
mesh_terms: D002460:Cell Line; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D016967:Fusobacterium nucleatum / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D009000:Monocytes / Q000187:drug effects* / Q000276:immunology*; D009055:Mouth / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects; D016966:Porphyromonas gingivalis / Q000378:metabolism; D013268:Stimulation, Chemical
chemical_list: D008070:Lipopolysaccharides; D016178:Granulocyte-Macrophage Colony-Stimulating Factor