Utilization of antipeptide antibodies as affinity ligands in immunoaffinity purification.
Anti-peptide antibodies against the C-terminal regions of chimeric alpha-amylase, recombinant CD2 and insulin B-chain were obtained by using peptides corresponding to the C-terminal regions as immunogens. These anti-peptide antibodies adsorbed the native proteins, as well as the antigen peptides. The proteins were purified to high purity using the anti-peptide antibodies as affinity ligands. These ligands could discriminate the target proteins having different C-terminal regions. The adsorbed proteins were specifically eluted by the eluents containing the antigen peptides.
Katoh
S
S
Department of Chemical Science and Engineering, Kobe University, Nada Rokkodai, Japan.
Terashima
M
M
Shiomi
N
N
D000327:Adsorption; D000345:Affinity Labels; D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D018801:CD2 Antigens / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D007328:Insulin / Q000276:immunology; D008024:Ligands; D010455:Peptides / Q000276:immunology*; D000516:alpha-Amylases / Q000276:immunology
D000345:Affinity Labels; D000906:Antibodies; D018801:CD2 Antigens; D007328:Insulin; D008024:Ligands; D010455:Peptides; D000516:alpha-Amylases