Neuropeptide Y receptors involved in calcium channel regulation in PC12 cells.
Our laboratory has previously used NGF-differentiated PC12 cells as a sympathetic neuronal model to investigate the effects of NPY on catecholamine synthesis and release. We have additionally used these cells to demonstrate the NPY-induced inhibition of Ca2+ channels which was suggested by those studies. In the present work, multiple NPY, PYY, and PP analogs are utilized to further define the receptor subtypes involved in this Ca2+ channel modulation. We find that in PC12 cells NPY and PP modulate Ca2+ channels through Y1, Y2, Y3, and Y4 receptors. In addition, we show that these receptors are differentially coupled to N, L, and non-N, non-L Ca2+ channel subtypes. The results of the present study in combination with our previous investigations demonstrate an intriguing and complex role for NPY and PP in the modulation of sympathetic neurotransmission.
McCullough
L A
LA
Department of Pharmacological and Physiological Science, Saint Louis University Health Sciences Center, MO 63104, USA.
Egan
T M
TM
Westfall
T C
TC
D000818:Animals; D001464:Barium / Q000378:metabolism; D015220:Calcium Channels / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D008959:Models, Neurological; D009478:Neuropeptide Y / Q000031:analogs & derivatives / Q000494:pharmacology; D016716:PC12 Cells; D010191:Pancreatic Polypeptide / Q000031:analogs & derivatives / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D019894:Peptide YY / Q000494:pharmacology; D051381:Rats; D017476:Receptors, Neuropeptide Y / Q000145:classification / Q000187:drug effects / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects / Q000502:physiology
D015220:Calcium Channels; D009478:Neuropeptide Y; D010446:Peptide Fragments; D017476:Receptors, Neuropeptide Y; D011994:Recombinant Proteins; D019894:Peptide YY; D001464:Barium; D010191:Pancreatic Polypeptide