[Method and significance of removal of dermal epithelial components in the study of composite skin].
In order to investigate the possible re-epithelialization originated from the dermal epithelial components after trypsinization of the allo-dermis, microscopic observation, tissue culture study and histological observation after allo-grafting were adopted. The number of the hair follicles in dermal layer was counted in the study process. The results showed that a few of hair follicles in the structure of allo-dermis remained after trypsin digestion for 80 minutes. After culturing and grafting of such trypsinized dermis, none of the newly formed epithelium was observed.


CONCLUSIONS
1. Trypsinization of allo-dermis eliminates the antigenic epithelial components of dermis preventing the possibility of re-epithelization; 2. Strictly controled time is required while digesting the allo-dermis with trypsin.
Cui
Z
Z
Department of Burns, Xijing Hospital, Fourth Military Medical University, Xi'an.
Chen
B
B
Shi
X
X
D000818:Animals; D000831:Animals, Newborn; D000078404:Epidermal Cells; D018859:Hair Follicle / Q000033:anatomy & histology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012867:Skin / Q000033:anatomy & histology*; D016038:Skin Transplantation / Q000379:methods*; D013552:Swine; D013997:Time Factors; D014183:Transplantation, Heterologous; D014357:Trypsin
D014357:Trypsin