title: Utilization of antipeptide antibodies as affinity ligands in immunoaffinity purification.
abstract: Anti-peptide antibodies against the C-terminal regions of chimeric alpha-amylase, recombinant CD2 and insulin B-chain were obtained by using peptides corresponding to the C-terminal regions as immunogens. These anti-peptide antibodies adsorbed the native proteins, as well as the antigen peptides. The proteins were purified to high purity using the anti-peptide antibodies as affinity ligands. These ligands could discriminate the target proteins having different C-terminal regions. The adsorbed proteins were specifically eluted by the eluents containing the antigen peptides.
authors.affiliation: Department of Chemical Science and Engineering, Kobe University, Nada Rokkodai, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Katoh
authors.forename: M
authors.initials: M
authors.lastname: Terashima
authors.forename: N
authors.initials: N
authors.lastname: Shiomi
mesh_terms: D000327:Adsorption; D000345:Affinity Labels; D000595:Amino Acid Sequence; D000906:Antibodies / Q000276:immunology*; D018801:CD2 Antigens / Q000276:immunology; D002846:Chromatography, Affinity / Q000379:methods*; D007328:Insulin / Q000276:immunology; D008024:Ligands; D010455:Peptides / Q000276:immunology*; D000516:alpha-Amylases / Q000276:immunology
chemical_list: D000345:Affinity Labels; D000906:Antibodies; D018801:CD2 Antigens; D007328:Insulin; D008024:Ligands; D010455:Peptides; D000516:alpha-Amylases