title: Generation of type-specific probes for the detection of single-copy human papillomavirus by a novel in situ hybridization method.
abstract: The integration of human papillomavirus (HPV) DNA is associated with the pathogenesis of HPV-associated malignancies. The ability, however, of standard in situ hybridization (ISH) to detect low-copy integrated HPV DNA is limited. We describe the generation of HPV type-specific biotin-labeled DNA probes and a novel ISH method that uses the catalyzed reporter deposition (CARD) system for the detection of single-copy target HPV DNA in formalin-fixed, paraffin-embedded tissue. Consensus primers flanking the noncoding region of HPVs were used to generate biotin-labeled HPV-6b, -11, -16 and -18 probes by polymerase chain reaction (PCR). The probes were used for ISH with the novel technique of CARD to increase the sensitivity of the assay. Tissue blocks were prepared from CaSki (500-600 copies of HPV-16), SiHa (1-2 copies of HPV-16), and HeLa (10-50 copies of HPV-18) cell lines, as well as from an HPV-negative cell line, C33A, and then tested to demonstrate the sensitivity and specificity of the probes. Surgical specimens were used to show the clinical applicability of this technique. We successfully detected HPV-16 DNA in CaSki and SiHa cells but not in HeLa or C33A cells. HPV-18 DNA was detected in HeLa cells but not in CaSki, SiHa, or C33A cells. Sensitivity was increased when ISH was performed using probes with more biotin incorporation or when more cycles of signal amplification were employed, but significant nonspecific background was observed after more than two cycles of signal amplification. The probes generated in this study detected specific types of HPV in surgical specimens with much higher sensitivity than did conventional ISH. We concluded that our new method was highly sensitive and could be applied to formalin-fixed, paraffin-embedded clinical material for the detection of HPV.
authors.affiliation: Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, USA. huangcc@adm.cgmh.com.tw
authors.forename: C C
authors.initials: CC
authors.lastname: Huang
authors.forename: J T
authors.initials: JT
authors.lastname: Qiu
authors.forename: M L
authors.initials: ML
authors.lastname: Kashima
authors.forename: R J
authors.initials: RJ
authors.lastname: Kurman
authors.forename: T C
authors.initials: TC
authors.lastname: Wu
mesh_terms: D002277:Carcinoma / Q000473:pathology / Q000821:virology; D016384:Consensus Sequence; D017931:DNA Primers / Q000737:chemistry; D015344:DNA Probes, HPV; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D007822:Laryngeal Neoplasms / Q000175:diagnosis / Q000821:virology; D010212:Papilloma / Q000473:pathology / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics*; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000821:virology*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014407:Tumor Cells, Cultured / Q000473:pathology / Q000821:virology; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000821:virology
chemical_list: D017931:DNA Primers; D015344:DNA Probes, HPV; D004279:DNA, Viral