[Experimental in vivo passage of H2 strain of live attenuated vaccine of hepatitis A in common marmosets].
Common marmosets with negative serum antibodies against hepatitis A virus (anti-HAV) and normal liver functions were used for the tests of passage of H2 vaccine strain of HAV to explore its residual virulence after attenuation and possibility of its reversion. Common marmosets were inoculated with attenuated live vaccine, and fecal suspension and/or proliferative liquid in tissue culture of the progeny of vaccine HAV excreted from their feces were passaged in common marmosets. Result of three serial passages in vivo indicated that there was no significant difference in serum anti-HAV titer, and no or low-level changes in activities of liver enzymes, no pathological changes in liver tissues observed, and detectable but minimal HAV was shed in stool of the all animals.
Huang
X
X
Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Kunming.
Lian
Y
Y
Wang
Q
Q
D000818:Animals; D002144:Callithrix; D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies / Q000097:blood*; D006507:Hepatovirus / Q000276:immunology*; D012692:Serial Passage / Q000379:methods; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines
D035922:Hepatitis A Antibodies; D022362:Hepatitis A Vaccines; D006508:Hepatitis Antibodies; D014613:Vaccines, Attenuated; D014761:Viral Hepatitis Vaccines