Brain administration of OB protein (leptin) inhibits neuropeptide-Y-induced feeding in ob/ob mice.
OB protein (or leptin) administration causes a long-lasting reduction in food intake and body weight in obese ob/ob mice. Neuropeptide Y, a stimulator of feeding, has been proposed to be a major mediator of the biological actions of OB protein. To test this hypothesis, the interaction of brain administration of exogenous OB protein and NPY on the feeding behavior of ob/ob mice was examined. Human OB protein, in a dose-dependent manner, partially or completely blocked feeding induced by exogenous NPY. These results demonstrate that OB protein can functionally antagonize and dominate the actions of exogenous NPY on feeding.
Smith
F J
FJ
Department of Metabolic Diseases, Hoffmann-La Roche Inc., Nutley, NJ 07110, USA. francoise_j.smith@roche.com
Campfield
L A
LA
Moschera
J A
JA
Bailon
P S
PS
Burn
P
P
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000502:physiology; D003346:Corticotropin-Releasing Hormone / Q000502:physiology; D004347:Drug Interactions; D004435:Eating / Q000187:drug effects* / Q000502:physiology; D005260:Female; D006801:Humans; D007276:Injections, Intraventricular; D020738:Leptin; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D009478:Neuropeptide Y / Q000008:administration & dosage* / Q000502:physiology; D009765:Obesity / Q000209:etiology / Q000503:physiopathology; D011333:Pro-Opiomelanocortin / Q000502:physiology; D011506:Proteins / Q000008:administration & dosage* / Q000502:physiology; D011994:Recombinant Proteins / Q000008:administration & dosage
D020738:Leptin; D009478:Neuropeptide Y; D011506:Proteins; D011994:Recombinant Proteins; D011333:Pro-Opiomelanocortin; D003346:Corticotropin-Releasing Hormone