title: Drosophila ferritin mRNA: alternative RNA splicing regulates the presence of the iron-responsive element.
abstract: Several mRNAs encoding the same ferritin subunit of Drosophila melanogaster were identified. Alternative RNA splicing and utilisation of different polyadenylation sites were found to generate the transcripts. The alternative RNA splicing results in ferritin transcripts with four unique 5' untranslated regions. Only one of them contains an iron-responsive element. The iron-responsive element was found to bind in vitro specifically to human recombinant iron regulatory protein 1. Furthermore, the ferritin subunit mRNAs are differentially expressed during development. Our data provides the first molecular evidence that the presence of iron-responsive element in a ferritin mRNA is regulated by alternative RNA splicing.
authors.affiliation: Department of Physiological Mycology, Uppsala University, Sweden.
authors.forename: M I
authors.initials: MI
authors.lastname: Lind
authors.forename: S
authors.initials: S
authors.lastname: Ekengren
authors.forename: O
authors.initials: O
authors.lastname: Melefors
authors.forename: K
authors.initials: K
authors.lastname: Söderhäll
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005293:Ferritins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015698:Genomic Library; D006801:Humans; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D011990:Receptors, Transferrin / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D035925:Iron-Regulatory Proteins; D007506:Iron-Sulfur Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011990:Receptors, Transferrin; D011994:Recombinant Proteins; D005293:Ferritins