title: Comparison of liquid chemical sterilization with peracetic acid and ethylene oxide sterilization for long narrow lumens.
abstract: The aim of this study was to determine how well peracetic acid liquid chemical sterilization (LCPAS) killed test organisms in the presence of 10% fetal bovine serum and 0.65% salt challenge (RPMI-S) compared with a 100% ethylene oxide (ETO) sterilizer and an ETO hydrochlorofluorocarbon (ETO-HCFC) sterilization method with long (125 cm), narrow (3-mm internal diameter) flexible lumens as the test carrier. The inoculated lumens were dried overnight before processing. The test organisms included Mycobacterium chelonei, Enterococcus faecalis, and Bacillus subtilis. For all 3 organisms tested, the LCPAS process resulted in a 6 log10 reduction in bacterial load compared with a 2.5 log10 to 6 log10 reduction for the 100% ETO and ETO-HCFC sterilizers. Sterilization was achieved for 100%, 61%, and 67% of the lumen test carriers for the LCPAS, 100% ETO, and ETO-HCFC sterilizers, respectively. The data indicate that of the sterilization methods evaluated, LCPAS was the most effective for sterilizing narrow flexible lumens in the presence of residual inorganic and organic soil. This effectiveness was achieved through a combination of organism wash-off and peracetic acid sterilant killing of organisms. Salt was the major compounding factor for effective ETO gas sterilization, because carriers inoculated with organisms in 10% fetal bovine serum alone all were sterilized by both 100% ETO and ETO-HCFC sterilization methods. Our data support the critical need to ensure adequate precleaning of narrow flexible lumen endoscopes before any sterilization method.
authors.affiliation: St Boniface Research Centre, Winnipeg, MB, Canada.
authors.forename: M J
authors.initials: MJ
authors.lastname: Alfa
authors.forename: P
authors.initials: P
authors.lastname: DeGagne
authors.forename: N
authors.initials: N
authors.lastname: Olson
authors.forename: R
authors.initials: R
authors.lastname: Hizon
mesh_terms: D001412:Bacillus subtilis / Q000254:growth & development; D004202:Disinfectants; D019723:Endoscopes / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000254:growth & development; D005027:Ethylene Oxide; D016926:Mycobacterium chelonae / Q000254:growth & development; D010463:Peracetic Acid; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid; D005027:Ethylene Oxide