Fibrinolytic and anticoagulative activities from the earthworm Eisenia foetida.
Biologically active glycolipoprotein complex (G-90) isolated from whole earthworm tissue extract shows anticoagulative and fibrinolytic activities. We isolated two tyrosine like serine peptidases with molecular masses of 34 kDa (P I) and 23 kDa (P II), respectively. P I peptidase is autocatalytically degraded to P II. Both peptidases exhibit fibrinolytic and anticoagulative activities. The activity of P I is much higher. P I in concentration of 10(5) ng ml-1 of plasma shortened the physiological time of fibrin clot lysis by 54% and completely inhibited blood clotting at a concentration of 10(3) ng ml-1 of venous blood.
Hrzenjak
T
T
Department of Biology, Faculty of Veterinary Medicine, University of Zagreb, Croatia.
Popović
M
M
Bozić
T
T
Grdisa
M
M
Kobrehel
D
D
Tiska-Rudman
L
L
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005337:Fibrin / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D015801:Helminth Proteins / Q000302:isolation & purification; D007700:Kinetics; D009835:Oligochaeta / Q000201:enzymology*; D012697:Serine Endopeptidases / Q000737:chemistry*; D014105:Tosyl Compounds / Q000494:pharmacology
D000925:Anticoagulants; D004791:Enzyme Inhibitors; D005343:Fibrinolytic Agents; D015801:Helminth Proteins; D014105:Tosyl Compounds; C003614:4-toluenesulfonyl fluoride; D005337:Fibrin; D012697:Serine Endopeptidases