Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei.
Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.
Cagampang
F R
FR
Anatomy and Human Biology, Division of Biomedical Sciences, King's College London, London, WC2R 2LS, UK.
Antoni
F A
FA
Smith
S M
SM
Piggins
H D
HD
Coen
C W
CW
D000262:Adenylyl Cyclases / Q000096:biosynthesis*; D000818:Animals; D001345:Autoradiography; D002940:Circadian Rhythm / Q000502:physiology*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D000262:Adenylyl Cyclases