title: Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum.
abstract: Yeasts of various genera were screened for denitrifying activity, and several yeast strains such as Trichosporon cutaneum, Fellomyces fuzhouensis, and Candida sp. were found to exhibit distinct activities to convert nitrite to nitrous oxide. Dissimilatory nitrite reductase (Nir) or nitric oxide reductase (Nor) activities were detected in the cell-free extracts of these yeasts. Spectrophotometric as well as Western blot analyses showed that T. cutaneum contains Nor of the cytochrome P450 type. This is the first report that shows that denitrification is also distributed among yeasts although their systems are incomplete, only capable of reducing nitrite to nitrous oxide.
authors.affiliation: Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Tsuruta
authors.forename: N
authors.initials: N
authors.lastname: Takaya
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Shoun
authors.forename: K
authors.initials: K
authors.lastname: Kimura
authors.forename: M
authors.initials: M
authors.lastname: Hamamoto
authors.forename: T
authors.initials: T
authors.lastname: Nakase
mesh_terms: D000693:Anaerobiosis; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004790:Enzyme Induction; D009572:Nitrite Reductases / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism*; D009609:Nitrous Oxide / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D013053:Spectrophotometry; D014250:Trichosporon / Q000145:classification / Q000201:enzymology* / Q000254:growth & development; D015003:Yeasts / Q000145:classification / Q000201:enzymology* / Q000378:metabolism
chemical_list: D009573:Nitrites; D003577:Cytochrome P-450 Enzyme System; D010088:Oxidoreductases; D009572:Nitrite Reductases; C080610:nitric-oxide reductase (P450); D009609:Nitrous Oxide