Neural and endogenous catecholamines in the bone marrow. Circadian association of norepinephrine with hematopoiesis?
Members of our research team have recently reported that adrenergic agents may affect hematopoiesis via alpha1-adrenoceptors present on bone marrow B cell precursors. In this paper we demonstrate that murine bone marrow contains a substantial amount of catecholamines. Norepinephrine (NE) and dopamine (DA) exhibited a daily rhythmicity, with peak values observed during the night. The rhythm was disrupted by chemical sympathectomy, whereas epinephrine (E) showed no rhythmicity or sensitivity to 6-hydroxydopamine. High and low values of NE and DA were associated with high and low values of their metabolites, which indicated a rhythmic catecholamine release. NE, but not DA or E, was positively associated with the proportion of cells in the G2/M and S phases of the cell cycle. Moreover, NE and DA were found in both short-term and long-term bone marrow cultures as well as in human or murine B lymphoid cell lines. These findings indicate that endogenous catecholamines in the bone marrow have both neural and cellular origins. The neural input shows a daily rhythm and may be implicated in the regulation of hematopoiesis.
Maestroni
G J
GJ
Center for Experimental Pathology, Cantonal Institute of Pathology, Locarno, Switzerland.
Cosentino
M
M
Marino
F
F
Togni
M
M
Conti
A
A
Lecchini
S
S
Frigo
G
G
D000818:Animals; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology; D001853:Bone Marrow / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000737:chemistry / Q000166:cytology; D002395:Catecholamines / Q000032:analysis / Q000096:biosynthesis*; D002453:Cell Cycle; D002478:Cells, Cultured / Q000737:chemistry; D002940:Circadian Rhythm; D004298:Dopamine / Q000032:analysis / Q000378:metabolism; D005260:Female; D006410:Hematopoiesis / Q000502:physiology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism / Q000502:physiology; D013997:Time Factors
D002395:Catecholamines; D004298:Dopamine; D009638:Norepinephrine