Induction of antitumor cytotoxic activity using CD34+ cord blood cell-derived and irradiated tumor cell-primed dendritic cells.
Dendritic cells (DCs) are the most powerful antigen-presenting cells in the immune system. They can activate immunologically naive T cells and improve the efficacy of immunotherapy against tumors. In the present study we investigated whether CD34+ cord blood cell-derived DCs are capable of inducing antitumor cytotoxic cells, such as cytotoxic T lymphocytes (CTLs), NK cells, and monocytes. Cord blood T cells stimulated by DCs pulsed with irradiated K562 major histocompatibility complex (MHC) class I+ cells were highly effective in eliciting a selective killing response against K562 class I+ cells. This killing activity was almost completely abrogated by antibodies to CD8 or MHC class I, but not to CD4. This suggests that tumor cell-pulsed DCs generated from CD34+ cord blood cells are able to induce tumor specific CTLs against corresponding tumor cells from cord blood T cells, and that these CTLs are CD8+ T cells which may recognize tumor cells via MHC class I molecules. This observation has potentially important implications for the use of DCs in clinical immunotherapy in cord blood transplantation.
Fujii
S
S
Second Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Fujimoto
K
K
Osato
M
M
Matsui
K
K
Takatsuki
K
K
Kawakita
M
M
D017951:Antigen Presentation / Q000276:immunology; D018952:Antigens, CD34 / Q000032:analysis; D000951:Antigens, Neoplasm / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000502:physiology; D003602:Cytotoxicity, Immunologic / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology* / Q000528:radiation effects; D005312:Fetal Blood / Q000166:cytology*; D015395:Histocompatibility Antigens Class I / Q000032:analysis; D006801:Humans; D007231:Infant, Newborn; D020014:K562 Cells / Q000276:immunology; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014407:Tumor Cells, Cultured
D018952:Antigens, CD34; D000951:Antigens, Neoplasm; D015395:Histocompatibility Antigens Class I