title: Mouse cortical neurones lacking APP show normal neurite outgrowth and survival responses in vitro.
abstract: We have cultured neurones from the developing cortex of mice that have had the amyloid precursor protein gene deleted (APP-null). Neurones cultured for a period of 24 h show similar neurite outgrowth and survival responses to wild-type neurones. Similar neurite outgrowth responses were also seen when neurones from APP-null mice were treated with a neurotrophic peptide derived from the APP sequence and compared with wild-type neurones. Finally, cortical cultures derived from APP-null mice showed similar survival responses to the toxic amyloid-beta peptide.
authors.affiliation: Department of Pharmacology, Merck, Sharp & Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK.
authors.forename: S J
authors.initials: SJ
authors.lastname: Harper
authors.forename: J G
authors.initials: JG
authors.lastname: Bilsland
authors.forename: M S
authors.initials: MS
authors.lastname: Shearman
authors.forename: H
authors.initials: H
authors.lastname: Zheng
authors.forename: L
authors.initials: L
authors.lastname: Van der Ploeg
authors.forename: D J
authors.initials: DJ
authors.lastname: Sirinathsinghji
mesh_terms: D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000494:pharmacology; D016564:Amyloid beta-Protein Precursor / Q000172:deficiency* / Q000235:genetics / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D048429:Cell Size / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D018345:Mice, Knockout; D008818:Mice, Neurologic Mutants; D008969:Molecular Sequence Data; D016501:Neurites / Q000502:physiology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor