Induction of sister chromatid exchanges by pyrimethamine in human lymphocyte cultures.
This study examined the effect of pyrimethamine (PYR) on sister chromatid exchanges (SCEs) in human lymphocytes. PYR is a folic acid antagonist used for the treatment of malaria and toxoplasmosis. PYR was added to peripheral blood lymphocyte cultures at three different doses: 0.05, 0.1, and 0.2 mg/ml. The results of the cytogenetic and statistical evaluations showed that the frequency of SCEs increased significantly in a dose-dependent manner. Thus, it is shown that PYR has a genotoxic effect on human chromosomes.
Egeli
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Department of Medical Biology, Faculty of Medicine, University of Uludag, Bursa, Turkey.
D000328:Adult; D000981:Antiprotozoal Agents / Q000633:toxicity*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D011739:Pyrimethamine / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects*
D000981:Antiprotozoal Agents; D011739:Pyrimethamine