title: The use of tailed octamer primers for cycle sequencing.
abstract: Studies have been carried out on the use of octamer oligonucleotides tailed with different base analogues as primers in cycle sequencing reactions. 5-Nitroindole tails improved the performance as primers of a number of octamers. A tail length of three or four 5-nitroindole residues significantly increased the sequencing signal intensity for almost all primers. The use of incomplete libraries of tailed octamer primers for primer walking is discussed.
authors.affiliation: Nycomed Amersham plc, Amersham Laboratories, White Lion Road, Amersham, Buckinghamshire HP7 9LL, UK.
authors.forename: S
authors.initials: S
authors.lastname: Ball
authors.forename: M A
authors.initials: MA
authors.lastname: Reeve
authors.forename: P S
authors.initials: PS
authors.lastname: Robinson
authors.forename: F
authors.initials: F
authors.lastname: Hill
authors.forename: D M
authors.initials: DM
authors.lastname: Brown
authors.forename: D
authors.initials: D
authors.lastname: Loakes
mesh_terms: D001483:Base Sequence; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics*; D007211:Indoles / Q000737:chemistry; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D007211:Indoles; C090067:5-nitroindole