[Sensitivity and specificity of the PCR-test-system for diagnosing HIV infections].
Sensitivity and specificity of the Russian PCR test system (experimental lot) were assessed with materials from 107 Russian patients with HIV infection and 100 donors without specific antibodies to HIV-1. The electrophoretic and hybridization-enzymatic methods for amplicon detection were compared. The sensitivity of electrophoresis was 89.9%, specificity 91%; for hybridization-enzymatic detection these values were 96.1 and 100%, respectively. Samples which yielded false-negative results were repeatedly tested by two-staged amplification (nested PCR) with external and internal primers. The main cause of false-negative results was a low concentration of proviral HIV-1 DNA in some blood samples.
Sarkisian
K A
KA
Shipulin
G A
GA
Vorob'eva
M S
MS
Shipulina
O Iu
OIu
Shakhgil'dian
V I
VI
Kravchenko
A V
AV
Buravtseva
E V
EV
Serebrovskaia
L V
LV
Pokrovskiĭ
V V
VV
Korystova
E V
EV
D015492:AIDS Serodiagnosis / Q000592:standards*; D004279:DNA, Viral / Q000097:blood; D005188:False Negative Reactions; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity
D004279:DNA, Viral