title: Phosphatidylglycerolphosphate synthase encoded by the PEL1/PGS1 gene in Saccharomyces cerevisiae is localized in mitochondria and its expression is regulated by phospholipid precursors.
abstract: The PEL1/PGS1 gene of the yeast Saccharomyces cerevisiae is essential for the viability of rho-/rho degrees mutants and the normal cardiolipin content of cells. The PEL1-GFP fusion gene has been found to complement the pel1/pgs1 mutation and its fluorescent protein was localized to mitochondria similarly to the beta-galactosidase activity of a protein encoded by the PEL1-lacZ fusion gene. The expression of the PEL1-lacZ reporter gene was repressed in cells grown in the presence of inositol and choline, reduced in the ino2 and ino4 strains, but constitutive in the opi1 null-mutant strain. The results demonstrate that Pel1p, playing a vital role in cells impaired in the mitochondrial DNA, is localized in the mitochondria and expressed in response to inositol and choline.
authors.affiliation: Department of Microbiology and Virology, Comenius University, Mlynská dolina B2, 842 15 Bratislava, Slovak Republic.
authors.forename: V
authors.initials: V
authors.lastname: Dzugasová
authors.forename: M
authors.initials: M
authors.lastname: Obernauerová
authors.forename: K
authors.initials: K
authors.lastname: Horváthová
authors.forename: M
authors.initials: M
authors.lastname: Vachová
authors.forename: M
authors.initials: M
authors.lastname: Záková
authors.forename: J
authors.initials: J
authors.lastname: Subík
mesh_terms: D002794:Choline / Q000494:pharmacology; D019286:Endopeptidase K / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D017930:Genes, Reporter / Q000235:genetics; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins; D007150:Immunohistochemistry; D007294:Inositol / Q000494:pharmacology; D007763:Lac Operon / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008928:Mitochondria / Q000201:enzymology* / Q000235:genetics; D016296:Mutagenesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics*; D014170:Transformation, Genetic / Q000235:genetics
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D007294:Inositol; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; D019286:Endopeptidase K; D002794:Choline