Characterization of a GTP-binding protein in the ADP-ribosylation factor subfamily from Leishmania tarentolae.
We report the cloning and characterization of a gene, LtARL, which encodes a small GTP-binding protein, from the protozoan Leishmania tarentolae. Hybridization analysis of genomic DNA under high stringency conditions indicates the single-copy nature of LtARL. LtARL is transcribed and yields a approximately 0.9 kb mRNA that is processed at the 5' end by trans-splicing. When expressed in Escherichia coli, LtArl binds GTP with a low stoichiometry and in a phospholipid-independent manner. Based on the greatest sequence identity with Homo sapiens Arl3 and lipid-independent binding of guanine nucleotides we designate this gene LtARL and the encoded protein LtArl.
Sturm
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NR
Department of Microbiology and Immunology, UCLA School of Medicine, 10833 Le Conte Avenue, Los Angeles, CA 90095-1747, USA.
Van Valkenburgh
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H
Kahn
R A
RA
Campbell
D A
DA
D020727:ADP-Ribosylation Factors; D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D017125:Genes, Protozoan; D007891:Leishmania / Q000235:genetics* / Q000378:metabolism; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D008565:Membrane Proteins; D011994:Recombinant Proteins; D000246:Adenosine Diphosphate Ribose; C097313:ADP-ribosylation factor related proteins; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors