title: Organization of fusicoccin receptor in higher plant plasma membrane: relationship between affinity and molecular mass
abstract: Higher plant plasma membranes carry receptors of different affinity for the phytotoxin fusicoccin. Reception of fusicoccin involves proteins belonging to the highly conserved 14-3-3 family, but the complete structure of the fusicoccin receptor (FCR) is unknown. Using radiation inactivation analysis, we estimated the molecular masses of low-affinity and high-affinity FCR at 63 +/- 7 and 130 +/- 15 kD, respectively. The dose dependences of receptor inactivation indicate that microsomal specimens contain "silent" FCRs of 420 +/- 90 kD in amounts commensurate with that of the active FCRs. Both low- and high-affinity FCRs are inactivated by hydrolytic enzymes from the outer surface of the plasma membrane, and impairment of protoplast integrity causes an irreversible transition of the low-affinity binding site into the high-affinity one. A scheme is proposed for the organization of different types of FCR in the plasma membrane, implying that the membrane affinity for fusicoccin reflects the interaction between proteins in the FCR complex.
authors.affiliation: Institute of Agricultural Biotechnology, Russian Academy of Agricultural Sciences, Moscow, 127550, Russia. avb@agrobio.msk.ru.
authors.initials: OI
authors.lastname: Klychnikov
authors.initials: AV
authors.lastname: Drabkin
authors.initials: OV
authors.lastname: Vasilenko
authors.initials: YS
authors.lastname: Pavlov
authors.initials: MS
authors.lastname: Trofimova
authors.initials: IN
authors.lastname: Smolenskaya
authors.initials: AA
authors.lastname: Rozenkranz
authors.initials: AS
authors.lastname: Sobolev
authors.initials: AV
authors.lastname: Babakov