[Investigation on the rate of urinary excretion of leptospires among cattle naturally infected with Leptospira interrogans].
Urine samples of Leptospires from cattle were detected by polymerase chain reaction (PCR) and isolation. Positive rates by Southern-blot and agarose gel electrophoresis of PCR amplification productions were 13% and 11% respectively. Positive rate of isolation was 3.1%. Various positive rates by PCR for cattle urine from different areas were discovered. Average rate of urinary excretion of Leptospires among cattle that naturally infected with Leptospira interrogans was 13.2%. Data showed that cattle was a major source of infection of Leptospirosis in some parts of China. According to the comparsion of results between PCR and isolation, we believe that PCR is a sensitive, rapid and simple method for the investigation on source of infection of Leptospirosis.
Shi
M H
MH
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Liang
Z X
ZX
Terpstera
W J
WJ
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000652:urine; D004197:Disease Reservoirs; D007921:Leptospira interrogans / Q000302:isolation & purification*; D007922:Leptospirosis / Q000382:microbiology / Q000662:veterinary*; D016133:Polymerase Chain Reaction; D014895:Weil Disease / Q000382:microbiology / Q000662:veterinary
D004269:DNA, Bacterial