title: Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: role of occludin in endothelial solute barriers.
abstract: OBJECTIVE
  The purpose of this study was to correlate the expression of occludin and VE-cadherin with the solute barrier properties of arterial and venous endothelial monolayers.
  
  
  METHODS
  Immunofluorescent confocal and traditional microscopy were used to determine junctional protein localization in endothelium in vivo and in vitro respectively, and western and northern analysis used to determine protein and gene expression levels. Permeability of endothelial monolayers was examined under normal, low calcium, and cytochalasin-D treatment conditions. Antisense oligonucleotide experiments for occludin were performed to determine the contribution of occludin to solute barrier.
  
  
  RESULTS
  Occludin protein in endothelial monolayers is more concentrated in arterial junctions than in venous junctions both in vivo and in vitro. Arterial endothelial cells express 18-fold more occludin protein and nine times more occludin mRNA compared to venous endothelial cells. In vivo, both endothelial cells demonstrate VE-cadherin staining; and in vitro, only venous endothelial cells express VE-cadherin protein and mRNA. Occludin antisense experiments suggest that both arterial and venous barrier properties are due to these different amounts of occludin expression. Venous barrier was remarkably sensitive to low extracellular calcium, while arterial barrier was more sensitive to cytochalasin-D.
  
  
  CONCLUSIONS
  These findings suggest strongly that arterial and venous endothelial barrier reflects the level of expression of different adhesion molecules and that modulation of these proteins, especially occludin, may regulate the level of endothelial solute barrier.
authors.affiliation: Department of Molecular and Cellular Physiology, LSU Medical Center, Shreveport 71130, USA.
authors.forename: C G
authors.initials: CG
authors.lastname: Kevil
authors.forename: N
authors.initials: N
authors.lastname: Okayama
authors.forename: S D
authors.initials: SD
authors.lastname: Trocha
authors.forename: T J
authors.initials: TJ
authors.lastname: Kalogeris
authors.forename: L L
authors.initials: LL
authors.lastname: Coe
authors.forename: R D
authors.initials: RD
authors.lastname: Specian
authors.forename: C P
authors.initials: CP
authors.lastname: Davis
authors.forename: J S
authors.initials: JS
authors.lastname: Alexander
mesh_terms: D015703:Antigens, CD; D001158:Arteries / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence; D015820:Cadherins / Q000235:genetics* / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D062793:Occludin; D020319:Oligodeoxyribonucleotides, Antisense / Q000235:genetics; D010539:Permeability; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism; D014680:Veins / Q000166:cytology / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D008565:Membrane Proteins; D062793:Occludin; D020319:Oligodeoxyribonucleotides, Antisense; D012333:RNA, Messenger; D000099152:Cadherin 5; C568985:OCLN protein, human