Typing of HLA-B*15 alleles using sequence-specific primers.
We have developed a DNA based typing method to detect 38 known B*15 alleles using sequence-specific primers (PCR-SSP). This method involves 38 primers and 39 PCR-SSP reactions with results that can be obtained in 3 hours. The method is easy, fast and suitable for clinical typing for bone marrow and organ transplantation. We have typed 106 HLA-B15 samples using this method. For homozygous HLA-B15 samples, some B*15 allele combinations need to be resolved by additional PCR reactions not included in this article. The method allows the detection of potential new alleles requiring sequencing for confirmation, and it is useful to resolve unusual serological reaction patterns for different HLA-B15 serological specificities. In addition, it could be used to resolve ambiguous PCR-SSOP typing results and for recognition of mismatches in serologically matched unrelated individuals.
Yu
N
N
American Red Cross Blood Services, New England Region, Dedham, MA 02026, USA.
Ohashi
M
M
Alosco
S
S
Salazar
M
M
Cao
K
K
Fernandez Vina
M
M
Yunis
E J
EJ
D000483:Alleles; D016026:Bone Marrow Transplantation; D017931:DNA Primers; D005006:Ethnicity / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016377:Organ Transplantation; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D017931:DNA Primers; D015235:HLA-B Antigens