Synergies between regulatory elements of the immunoglobulin heavy chain locus and its palindromic 3' locus control region.
Transcriptional enhancers of the IgH locus include E mu and four 3' elements (C alpha3', hs1-2, hs3 and hs4), some of which are by themselves weak. We show that these weak elements behave as strong "co-enhancers" when combined, and display a stage-dependent activity which differs from that obtained when they are alone. Combinations mimicking the palindromic structure of the 3' IgH region are particularly efficient. Noticeably in pre-B cells, hs4 is boosted by the addition of elements previously considered inactive at this stage, hs1-2 and hs3. Combinations of 3' elements also strongly boost E mu at all maturation stages, but inhibitory interactions occasionally occur between E mu and incomplete 3' combinations, indicating that full transcriptional activity is mainly achieved when all 5' and 3' partners play their respective roles.
Chauveau
C
C
Laboratoire d'Immunologie, CNRS EP118, Faculté de Médecine, Limoges, France.
Pinaud
E
E
Cogne
M
M
D001402:B-Lymphocytes / Q000378:metabolism; D002460:Cell Line; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D005803:Genes, Immunoglobulin; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D019169:Jurkat Cells; D013601:T-Lymphocytes; D014158:Transcription, Genetic
D007143:Immunoglobulin Heavy Chains