title: Chromatin conformation in living cells: support for a zig-zag model of the 30 nm chromatin fiber.
abstract: A new method was used to probe the conformation of chromatin in living mammalian cells. The method employs ionizing radiation and is based on the concept that such radiation induces correlated breaks in DNA strands that are in spatial proximity. Human dermal fibroblasts in G0 phase of the cell cycle and Chinese hamster ovary cells in mitosis were irradiated by X-rays or accelerated ions. Following lysis of the cells, DNA fragments induced by correlated breaks were end-labeled and separated according to size on denaturing polyacrylamide gels. A characteristic peak was obtained for a fragment size of 78 bases, which is the size that corresponds to one turn of DNA around the nucleosome. Additional peaks between 175 and 450 bases reflect the relative position of nearest-neighbor nucleosomes. Theoretical calculations that simulate the indirect and direct effect of radiation on DNA demonstrate that the fragment size distributions are closely related to the chromatin structure model used. Comparison of the experimental data with theoretical results support a zig-zag model of the chromatin fiber rather than a simple helical model. Thus, radiation-induced damage analysis can provide information on chromatin structure in the living cell.
authors.affiliation: Lawrence Berkeley National Laboratory, University of California, One Cyclotron Road, MS29-100, Berkeley, CA, 94720, USA.
authors.forename: B
authors.initials: B
authors.lastname: Rydberg
authors.forename: W R
authors.initials: WR
authors.lastname: Holley
authors.forename: I S
authors.initials: IS
authors.lastname: Mian
authors.forename: A
authors.initials: A
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D016466:CHO Cells / Q000528:radiation effects; D002843:Chromatin / Q000737:chemistry* / Q000528:radiation effects; D003198:Computer Simulation; D006224:Cricetinae; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005347:Fibroblasts / Q000528:radiation effects; D006801:Humans; D008938:Mitosis; D008954:Models, Biological; D008958:Models, Molecular; D008967:Molecular Biology / Q000379:methods*; D009707:Nucleosomes / Q000737:chemistry / Q000378:metabolism
chemical_list: D002843:Chromatin; D004277:DNA, Single-Stranded; D009707:Nucleosomes