title: Deletion of the 389 N-terminal residues of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans.
abstract: Utilizing a homologous gene replacement in order to retain the native promoter and 5' and 3' untranslated messenger regions (and thereby ensure physiological validity), we have shown that deletion of the N-terminal 389 amino acids of the transcriptional activator AREA does not result in nitrogen metabolite derepression in Aspergillus nidulans. Our results provide no evidence for a modulating interaction involving the N terminus of AREA and contrast with those of H. K. Lamb, A. L. Dodds, D. R. Swatman, E. Cairns, and A. R. Hawkins (J. Bacteriol. 179:6649-6656, 1997), who used nontargeted ectopic copies of a construct containing a heterologous promoter and untranslated regions. Results obtained with this deletion mutant, nevertheless, provide further evidence for the dispensability of large portions of AREA.
authors.affiliation: School of Biological Sciences, Donnan Laboratories, University of Liverpool, Liverpool L69 7ZD, United Kingdom.
authors.forename: M X
authors.initials: MX
authors.lastname: Caddick
authors.forename: H N
authors.initials: HN
authors.lastname: Arst
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D009584:Nitrogen / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C079728:AreA protein, Aspergillus nidulans; D005656:Fungal Proteins; D015534:Trans-Activators; D014157:Transcription Factors; D009584:Nitrogen