title: Epitope analysis of staphylococcal enterotoxin A using different synthetic peptides.
abstract: The antigenic determinants (or epitopes) of staphylococcal enterotoxin A (SEA) were analyzed using synthetic peptides and rabbit antibodies to their corresponding peptides. Of 12 different synthetic peptides tested, peptides A-1 (corresponding to the amino acid sequence 1-20), A-5 (81-100), and A-8 (141-160) were reactive with anti-SEA antibodies. However, all synthetic peptides were found to elicit antibodies reactive with native SEA molecule. These findings suggest that native SEA molecule contains at least 3 different antigenic determinants.
authors.affiliation: Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
authors.forename: D L
authors.initials: DL
authors.lastname: Hu
authors.forename: A
authors.initials: A
authors.lastname: Imai
authors.forename: K
authors.initials: K
authors.lastname: Ono
authors.forename: S
authors.initials: S
authors.lastname: Sasaki
authors.forename: A
authors.initials: A
authors.lastname: Nakane
authors.forename: S
authors.initials: S
authors.lastname: Sugii
authors.forename: K
authors.initials: K
authors.lastname: Shinagawa
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; C026066:enterotoxin A, Staphylococcal