[Transport into the cell nucleus of p42 and p44 isoforms of MAP kinase induced by epidermal growth factor requires the presence of intact active cytoskeleton].
The influence of cytoskeleton-depolymerising agents (nocodazole and cytochalasine D) on EGF-induced transport of p42/p44MAPK into the cell nucleus was investigated. Using immunoblotting and immunofluorescence methods we have shown that depolymerization of microtubules does not affect EGF-induced nuclear import of MAPK, while actin depolymerization significantly inhibited this process. At the same time, cytochalasine D did not affect the EGF-induced MAPK activation in the cytoplasm. These data indicate that the EGF-induced nuclear import of MAPK depends on the microfilament system.
Arnautov
A M
AM
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Nikol'skiĭ
N N
NN
D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002467:Cell Nucleus / Q000201:enzymology; D003599:Cytoskeleton / Q000201:enzymology; D004815:Epidermal Growth Factor / Q000502:physiology*; D006801:Humans; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases
D004815:Epidermal Growth Factor; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases