Potato protein kinase StCPK1: a putative evolutionary link between CDPKs and CRKs.
Calcium-dependent protein kinases (CDPKs) in plants are characterized by a four-domain structure including conserved sequences in the catalytic domain, and in the C-terminal calmodulin-like domain. Based on this conservation we have PCR-amplified and isolated a potato cDNA clone (StCPK1) from a library representing an early stage of tuber development. DNA sequence analysis revealed that in the catalytic domain, StCPK1 shares more homology with CDPK-related kinases than with CDPKs; however, like CDPKs, it possesses canonical EF-hands at the calmodulin-like 3' end. StCPK1 exists in a few copies in the potato genome and is abundantly expressed in the sepals of mature flowers. Floral expression of genes homologous to StCPK1 appears to be widespread in the family Solanaceae.
Lakatos
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Agricultural Biotechnology Center, P.O. Box 411, H-2101 Gödöllo, Hungary.
Hutvágner
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G
Bánfalvi
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Z
D000595:Amino Acid Sequence; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000737:chemistry / Q000235:genetics*; D002147:Calmodulin / Q000737:chemistry; D020134:Catalytic Domain; D003001:Cloning, Molecular; D017931:DNA Primers; D019143:Evolution, Molecular; D015723:Gene Library; D008969:Molecular Sequence Data; D005810:Multigene Family; D010944:Plants / Q000201:enzymology; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D011198:Solanum tuberosum / Q000201:enzymology* / Q000235:genetics*
D002147:Calmodulin; D017931:DNA Primers; D017871:Calcium-Calmodulin-Dependent Protein Kinases