Effect of 26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 on the expression of vitamin-D-responsive genes in vitamin-D-deficient mice.
26,26,26,27,27,27-Hexafluoro-1,25-dihydroxyvitamin D3 (ST-630) is a newly developed agent to maintain the levels of calcium and phosphorus in blood. Herein, we investigated the effect of this compound on the expression of vitamin-D-responsive genes in vitamin-D-deficient mice. ST-630 was more effective than 1, 25-dihydroxyvitamin D3 [1,25(OH)2D3] with respect to the induction of Cyp24 and calbindin-D9k mRNAs in the kidney and in the small intestine. Moreover, the increase in mRNA levels of vitamin-D-responsive genes induced by ST-630 lasted longer than that induced by 1,25(OH)2D3. These results indicate that ST-630 was more effective in inducing Cyp24 and calbindin-D9k gene expression than 1, 25(OH)2D3 when both compounds were injected into vitamin-D-deficient mice.
Yoshimura
T
T
Faculty of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Itoh
S
S
Tsujikawa
K
K
Yamada
E
E
Ishii
T
T
Iemura
O
O
Kameda
Y
Y
Mimura
T
T
Kohama
Y
Y
D000818:Animals; D064026:Calbindins; D002117:Calcitriol / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use*; D002118:Calcium / Q000097:blood; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis; D004790:Enzyme Induction / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D007275:Injections, Intravenous; D007421:Intestine, Small / Q000187:drug effects* / Q000201:enzymology; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012333:RNA, Messenger / Q000235:genetics; D064030:S100 Calcium Binding Protein G / Q000187:drug effects / Q000235:genetics; D013250:Steroid Hydroxylases / Q000096:biosynthesis; D014807:Vitamin D / Q000235:genetics* / Q000378:metabolism; D014808:Vitamin D Deficiency / Q000097:blood / Q000188:drug therapy*; D065668:Vitamin D3 24-Hydroxylase; D005466:Fluorocarbons
D064026:Calbindins; D002117:Calcitriol; D002118:Calcium; D003577:Cytochrome P-450 Enzyme System; D012333:RNA, Messenger; D064030:S100 Calcium Binding Protein G; D013250:Steroid Hydroxylases; D014807:Vitamin D; D065668:Vitamin D3 24-Hydroxylase; C576507:S100g protein, mouse; C040488:26,26,26,27,27,27-hexafluoro-1,25-dihydroxyvitamin D3; D005466:Fluorocarbons