title: Molecular analysis of phosphoglycerate kinase in Trypanoplasma borreli and the evolution of this enzyme in kinetoplastida.
abstract: In the protozoan kinetoplastid organism Trypanoplasma borreli, phosphoglycerate kinase (PGK) activity was found in two different cell compartments: 80% in the cytosol and 20% in peroxisome-like organelles called glycosomes. However, only one functional pgk gene could be detected, in addition to a pseudo-pgk gene. No short-range linkage could be established between these two genes, although they are presumably present on the same chromosome. The intact gene codes for a polypeptide of 411 amino acids, with a C-terminal extension of four residues, -VAKF, a sequence with probably a low targeting efficiency for glycosomes. The calculated net charge and molecular mass of the encoded polypeptide are +13 and 44230Da, respectively. In other Kinetoplastida, different tandemly arranged genes code for distinct PGK isoenzymes in glycosomes and cytosol. By comparison of the pgk gene organization, and a phylogenetic analysis, we have traced a plausible scenario of the evolution of the PGK isoenzymes in these organisms and of the enzymes' intracellular compartmentation.
authors.affiliation: Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology (ICP), Laboratory of Biochemistry, Catholic University of Louvain (UCL), Brussels, Belgium.
authors.forename: C A
authors.initials: CA
authors.lastname: Adjé
authors.forename: F R
authors.initials: FR
authors.lastname: Opperdoes
authors.forename: P A
authors.initials: PA
authors.lastname: Michels
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D016830:Kinetoplastida / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014351:Trypanosomatina / Q000201:enzymology / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase