Cats are protected against feline immunodeficiency virus infection following vaccination with a homologous AP-1 binding site-deleted mutant.
Following establishment, via the vaginal route, of infection with an AP-1 binding-site deleted mutant (delta AP-1) of feline immunodeficiency virus (FIV), cats were challenged with a homologous intact strain (TM2) of FIV. The cats were observed for 23 weeks to evaluate the efficacy of the delta AP-1 against the homologous TM2 strain challenge. These two viruses were differentiated by Southern blotting after amplification of proviral DNA by semi-nested polymerase chain reaction in DNAs of peripheral blood mononuclear cells and tissues. A TM2-specific band was detected in one cat exposed to but not infected with delta AP-1, but not in two delta AP-1-infected. These results indicate that delta AP-1 could protect against subsequent challenge with homologous FIV TM2 strain.
Kohmoto
M
M
Department of Veterinary Microbiology, University of Tokyo, Japan.
Miyazawa
T
T
Sato
E
E
Uetsuka
K
K
Nishimura
Y
Y
Ikeda
Y
Y
Inada
G
G
Doi
K
K
Mikami
T
T
D000818:Animals; D001665:Binding Sites; D002415:Cats; D004279:DNA, Viral / Q000032:analysis; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D005260:Female; D009154:Mutation; D016133:Polymerase Chain Reaction; D011533:Proviruses / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000276:immunology*; D014611:Vaccination; D014623:Vaginal Diseases / Q000517:prevention & control*; D014765:Viral Vaccines / Q000276:immunology*
D004279:DNA, Viral; D018808:Transcription Factor AP-1; D014765:Viral Vaccines