Secretory immune response in patients with intestinal amoebiasis.
The secretory immune response in saliva from intestinal amoebiasis patients against antigens obtained from Entamoeba histolytica membranes was studied. Western blot analysis indicated that patient saliva contains secretory IgA antibodies against antigens with molecular masses ranging from 170 to 24 kDa, some of which were also recognized by saliva from healthy subjects. However, antigens of 170, 125, 46 and 37 kDa are recognized more frequently (> 90%) by the secretory IgA from patients with intestinal amoebiasis than by that from healthy subjects (< 10%).
Ortiz-Ortiz
L
L
Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México.
Mora
N
N
Zambrano-Villa
S A
SA
Carrero
J C
JC
Sánchez-Zerpa
M
M
Osuna
A
A
Rosales-Borjas
D M
DM
D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis; D000953:Antigens, Protozoan / Q000302:isolation & purification; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000276:immunology*; D006801:Humans; D015151:Immunoblotting; D007071:Immunoglobulin A, Secretory / Q000032:analysis*; D007411:Intestinal Diseases, Parasitic / Q000276:immunology*; D008565:Membrane Proteins / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000302:isolation & purification; D012463:Saliva / Q000276:immunology*
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory; D008565:Membrane Proteins; D015800:Protozoan Proteins