Differential release of endotoxin from Pseudomonas aeruginosa treated with beta-lactam antibiotics and its effect on the lethal activity.
The relationship between the level of endotoxin released from Pseudomonas aeruginosa by exposure to beta-lactam antibiotics and the lethal activity against D-galactosamine (D-GalN)-sensitized mice was examined. In vitro treatment of P. aeruginosa with imipenem (IPM) exclusively caused low-level release of free endotoxin, which was not lethal for D-GalN-sensitized mice. Treatment with other beta-lactam antibiotics, such as ceftazidime (CAZ), meropenem (MEPM) and cefozopran (CZOP) caused high-level release of free endotoxin and it exhibited the lethal action against D-GalN-sensitized mice. This study demonstrated close association of free endotoxin released by antibiotic treatment with the lethal activity.
Narita
K
K
Department of Microbiology and Immunology, Aichi Medical University.
Koide
N
N
Morikawa
A
A
Kato
Y
Y
Sugiyama
T
T
Yoshida
T
T
Yokochi
T
T
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D005688:Galactosamine; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D013237:Stereoisomerism; D047090:beta-Lactams
D000900:Anti-Bacterial Agents; D008070:Lipopolysaccharides; D047090:beta-Lactams; D005688:Galactosamine