[The value of DNA flow cytometry in evaluation of proliferating activity of neoplasm cells in patients with laryngeal cancer].
A study of DNA ploidy was carried out including 84 cases of laryngeal squamous cell carcinoma. Material was fixed during 30 min in 3% paraformaldehyde and embedded in low-melting-point paraffin. The histologic studies were done for diagnosis and general view. Results were as follows. The highest percentage of cancers with DNA diploidy was found in well- and medium-differentiated tumors. Among well-differentiated carcinomas there were 18% diploid versus 5% polyploid tumors. In the medium-differentiated cancers there were 23.5% diploid, 6% polyploid, and 6% hyperdiploid tumors. The highest percentage of DNA hyperdiploidy and poliploido-aneuploidy was found in low-differentiated cancers. Additionally, tumor proliferative activity was evaluated in relation to the percentage of cells in phase S of cellular cycle. Special attention was paid to tumors which revealed the phenomenon of DNA poliploidy-aneuploidy beside DNA diploidy.
Golusiński
W
W
Katedra i Klinika Otolaryngologii AM w Poznaniu.
Szmeja
Z
Z
Krygier-Stojałowska
A
A
Biczysko
W
W
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D002465:Cell Movement / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D009360:Neoplastic Cells, Circulating; D011003:Ploidies