Cloning and sequence analysis of the gene encoding invertase (INV1) from the yeast Candida utilis.
The gene INV1 encoding invertase from the yeast Candida utilis has been cloned using a homologous PCR hybridization probe, amplified with two sets of degenerate primers designed considering sequence comparisons between yeast invertases. The cloned gene was sequenced and found to encode a polypeptide of 533 amino acids that contain a 26 amino-acid signal peptide and 12 potential N-glycosylation sites. The nucleotide sequences of the 5' and 3' non-coding regions were found to contain motifs probably involved in initiation, regulation and termination of gene transcription. The amino-acid sequence shows significant identity with other yeast, bacterial and plant beta-fructofuranosidases. The INV1 gene from C. utilis was able to complement functionally the suc2 mutation of S. cerevisiae.
Chávez
F P
FP
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Pons
T
T
Delgado
J M
JM
Rodríguez
L
L
D000595:Amino Acid Sequence; D001483:Base Sequence; D015139:Blotting, Southern; D002175:Candida / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D043324:beta-Fructofuranosidase
D017931:DNA Primers; D021382:Protein Sorting Signals; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase