title: Apoptotic cell death in human granulosa-lutein cells: a possible indicator of in vitro fertilization outcome.
abstract: OBJECTIVE
  To determine whether women who do not conceive during an IVF treatment despite normal FSH levels have a higher rate of apoptosis in their granulosa-lutein cells than women who do conceive.
  
  
  METHODS
  Prospective.
  
  
  METHODS
  Large teaching hospital and fertility center.
  
  
  METHODS
  Patients with normal FSH levels undergoing an IVF treatment.
  
  
  METHODS
  None.
  
  
  METHODS
  Outcome of the IVF treatment and percentage of apoptotic granulosa-lutein cells.
  
  
  RESULTS
  A significantly lower percentage of granulosa-lutein cells were apoptotic in patients who became pregnant compared with those who did not become pregnant. A higher basal FSH level was significantly correlated with the duration of the stimulation, and the number of follicles was significantly inversely correlated with the number of ampules of FSH used and the duration of the treatment. The number of embryos was significantly correlated with the number of oocytes, and significantly inversely correlated with the number of apoptotic granulosa-lutein cells.
  
  
  CONCLUSIONS
  Fewer granulosa-lutein cells are apoptotic in women who have an ongoing pregnancy after IVF treatment than in women who do not conceive.
authors.affiliation: Department of Obstetrics and Gynecology, Medisch Spectrum Twente Hospital Group, Amsterdam, The Netherlands.
authors.forename: G J
authors.initials: GJ
authors.lastname: Oosterhuis
authors.forename: H W
authors.initials: HW
authors.lastname: Michgelsen
authors.forename: C B
authors.initials: CB
authors.lastname: Lambalk
authors.forename: J
authors.initials: J
authors.lastname: Schoemaker
authors.forename: I
authors.initials: I
authors.lastname: Vermes
mesh_terms: D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry; D006107:Granulosa Cells / Q000473:pathology*; D006801:Humans; D008184:Luteal Cells / Q000473:pathology*; D011247:Pregnancy; D011379:Prognosis; D011446:Prospective Studies; D016896:Treatment Outcome