Increased metabolism of peroxynitrite-modified [125I] beta-VLDL by monocytes from hypercholesterolemic rabbits.
The metabolism (uptake and degradation) of peroxynitrite-modified beta-VLDL and native beta-VLDL labeled with [125I]iodine was studied in monocytes isolated from peripheral blood of hypercholesterolemic (HC) and normolipidemic rabbits (NC). The peroxynitrite-modified beta-VLDL uptake and degradation was up to 2-fold higher in monocytes from HC rabbits than monocytes from NC rabbits. In addition, monocytes from HC rabbits took up and degraded significantly more peroxynitrite-modified beta-VLDL than native beta-VLDL. In contrast, monocytes from NC rabbits took up both lipoproteins in a similar rate and degraded more native beta-VLDL than peroxynitrite-modified beta-VLDL. Our results suggest that hypercholesterolemia can affect monocyte metabolism enhancing foam cell formation by increasing the influx of peroxynitrite-modified beta-VLDL.
da Silva
E L
EL
Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences (FCFUSP), University of São Paulo, Brazil.
Abdalla
D S
DS
D000818:Animals; D002784:Cholesterol / Q000097:blood; D002791:Cholesterol, Dietary / Q000008:administration & dosage; D006937:Hypercholesterolemia / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D009566:Nitrates / Q000494:pharmacology*; D011817:Rabbits; D014280:Triglycerides / Q000097:blood
D002791:Cholesterol, Dietary; D008079:Lipoproteins, VLDL; D009566:Nitrates; D014280:Triglycerides; C039642:peroxynitric acid; D002784:Cholesterol