Analysis of variable number of tandem repeats of human genome D1S80 locus using capillary electrophoresis with laser-induced fluorescence detector.
The conventional method of identifying individuals by DNA in the field of forensic medicine is slab gel electrophoresis, which is time-consuming, labor-intensive, and nonquantitative. Accordingly, the use of capillary electrophoresis with a laser-induced fluorescence detector (CE-LIF), human genome D1S80 locus, a DNA marker which has a variable number of tandem repeats (VNTR) on chromosome 1, was examined to improve DNA analysis for identification. Using an internal standard, fragment size of VNTR was accurately and rapidly determined.
Nishimura
A
A
Department of Functional Molecular Chemistry, Kobe Pharmaceutical University, Japan.
Tsuhako
M
M
Baba
Y
Y
D004247:DNA / Q000032:analysis* / Q000235:genetics; D019075:Electrophoresis, Capillary; D005819:Genetic Markers; D015894:Genome, Human; D006801:Humans; D007834:Lasers; D018598:Minisatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
D005819:Genetic Markers; D004247:DNA