The effect of prednisolone on canine neutrophil function: in vivo and in vitro studies.
The in vivo effect of a therapeutic dose of prednisolone on canine neutrophil adherence, random migration, chemotaxis, phagocytosis of IgG and C3b opsonized yeast cells, chemiluminescence, Fc- and CR3-receptor expression was investigated. Prednisolone was also added in vitro to neutrophils as isolated cells and in whole blood. In the in vivo study, prednisolone increased the IgG mediated ingestion of yeast cells and the number of activated neutrophils in the phagocytosis assay, while flow cytometric investigation of the IgG-receptor Fc gamma RIII with a monoclonal antibody showed similar expression before, during and after treatment. Prednisolone also increased the ingestion of C3b-opsonized yeast cells, while the expression of CR3-receptors (CD11b CD18) measured by flow cytometry was unchanged. Chemiluminescence and the chemotactic response towards zymosan activated serum were increased, while adherence to nylon wool was decreased. The in vitro studies revealed that prednisolone had no or a dampening effect on neutrophils in cell suspensions. Adherence as well as IgG mediated ingestion was decreased at the highest prednisolone concentration (800 ng/ml) in whole blood. The present study suggests that the part of the antiinflammatory effect of corticosteroids mediated through their influence on neutrophils, besides reduced adherence, may be exerted by increased clearance of microorganisms and IgG-complexes through an elevated functional capacity.
Trowald-Wigh
G
G
Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Uppsala, Sweden. Gunilla.Trowald-Wigh@kirmed.slu.se
Håkansson
L
L
Johannisson
A
A
Edqvist
L E
LE
D000818:Animals; D002478:Cells, Cultured; D002633:Chemotaxis / Q000187:drug effects*; D004285:Dogs; D005260:Female; D005434:Flow Cytometry; D005938:Glucocorticoids / Q000494:pharmacology*; D007104:Immune Adherence Reaction; D008297:Male; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D011239:Prednisolone / Q000494:pharmacology*
D005938:Glucocorticoids; D011239:Prednisolone