Purification of first-generation Eimeria tenella schizonts.
A rapid and simple method for purifying first-generation Eimeria tenella schizonts was developed with infected chicken cecal tissue. The schizonts were harvested from the tissue by treatment with a mixture of 3 enzymes: hyaluronidase, dispase, and collagenase. Subsequent purification of the parasites by filtration through gauze and centrifugation resulted in a clean final preparation compared with the starting material. Microscopical and biochemical examinations, performed to assess the quality of the schizont purification, showed a high degree of purity. This procedure may be used to obtain clean schizonts minimally contaminated with host-cell debris in order to study this parasitic stage.
Ouarzane
M
M
Unité de Virologie et Immunologie Moléculaires, INRA, Jouy-en-Josas, France.
Labbé
M
M
Péry
P
P
D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis / Q000276:immunology; D015153:Blotting, Western / Q000662:veterinary; D002432:Cecum / Q000378:metabolism / Q000469:parasitology*; D002498:Centrifugation / Q000662:veterinary; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D017364:Collagenases / Q000378:metabolism; D016786:Eimeria tenella / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D010450:Endopeptidases / Q000378:metabolism; D005374:Filtration; D006821:Hyaluronoglucosaminidase / Q000378:metabolism; D007106:Immune Sera / Q000276:immunology; D051379:Mice; D011201:Poultry Diseases / Q000469:parasitology*; D015800:Protozoan Proteins / Q000032:analysis / Q000276:immunology; D016053:RNA, Protozoan / Q000032:analysis; D013997:Time Factors
D000953:Antigens, Protozoan; D007106:Immune Sera; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D006821:Hyaluronoglucosaminidase; D010450:Endopeptidases; D017364:Collagenases; C023139:dispase