Spontaneous activity of preoptic neurons in slice preparations of the hypothalamus of European hamsters (Cricetus cricetus) and Wistar rats under different states of hypothermia.
Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 and 57% of spontaneously active neurons in the medial preoptic area were classified as warm-sensitive in rats and hamsters, respectively. With decreasing slice temperature, the number of active neurons decreased progressively without a significant difference between rats and hamsters. At a slice temperature of 10 degreesC, 57% of all hypothalamic neurons in rats and 42% in hamsters were still spontaneously active. The average temperature at which activity ceased completely when the temperature was decreased further (the cut-off temperature) was 7.9 +/- 0.3 degreesC (n = 14) in rats but was significantly lower at 4.9 +/- 0.4 degreesC (n = 8) in hamsters (P < 0.001). Firing rates and temperature coefficients did not differ in their temperature dependence between rats and hamsters. Action potential duration increased with decreasing slice temperature in both species, but the increase in duration was significantly greater in rats.
Hashimoto
M
M
Max-Planck-Institut für Physiologische und Klinische Forschung, W. G. Kerckhoff-Institut, Parkstrasse 1, Bad Nauheim, D-61231, Germany. mhashi@asahikawa-med.ac.jp
Schmid
H A
HA
Ohwatari
N
N
Pleschka
K
K
D000200:Action Potentials; D000818:Animals; D001833:Body Temperature Regulation / Q000502:physiology; D006224:Cricetinae; D006605:Hibernation / Q000502:physiology*; D007035:Hypothermia / Q000503:physiopathology*; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000502:physiology*; D011301:Preoptic Area / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D013045:Species Specificity; D013696:Temperature