Study of the O-acetylesterase activity of five influenza C virus strains.
Four influenza C virus strains, isolated in France in 1991, were used as a source for a kinetic study of the enzyme O-acetylesterase (EC 3.1.1.53) related to another strain, C/JHB/1/66, considered as the reference strain. Similarities, but also differences, in their haemagglutination titres were detected. Remarkable differences were found for enzyme activity and the K(m), Vmax, and the Vmax/K(m) ratio between certain strains, as well as for their thermostability at 40 degrees C when methylumbelliferyl acetate was used as substrate. By contrast, their optimum pH, stability at different pH values, and stability at 4 degrees C over 14 days were very similar. The effect of some compounds on O-acetylesterase activity was studied. The peculiarities of these factors are discussed in relation to the functional variation of the virus.
Sánchez-Bernal
C
C
Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Salamanca, Salamanca, Spain.
Muñoz-Barroso
I
I
Manuguerra
J C
JC
Hannoun
C
C
Cabezas
J A
JA
D000115:Acetylesterase / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D009982:Gammainfluenzavirus / Q000201:enzymology*; D007700:Kinetics
D000115:Acetylesterase