title: Effects of basic fibroblast growth factor on proliferation and phenotype expression of chondrocytes embedded in collagen gel.
abstract: 1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.
authors.affiliation: Department of Orthopaedics, Shimane Medical University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Matsusaki
authors.forename: M
authors.initials: M
authors.lastname: Ochi
authors.forename: Y
authors.initials: Y
authors.lastname: Uchio
authors.forename: N
authors.initials: N
authors.lastname: Shu
authors.forename: H
authors.initials: H
authors.lastname: Kurioka
authors.forename: K
authors.initials: K
authors.lastname: Kawasaki
authors.forename: N
authors.initials: N
authors.lastname: Adachi
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002809:Chondroitin Sulfates / Q000037:antagonists & inhibitors; D003094:Collagen; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005782:Gels; D007150:Immunohistochemistry; D010641:Phenotype; D011817:Rabbits; D016610:Tissue Embedding
chemical_list: D003470:Culture Media; D005782:Gels; D016222:Fibroblast Growth Factor 2; D002809:Chondroitin Sulfates; D003094:Collagen