Patch-clamp and amperometric recordings from norepinephrine transporters: channel activity and voltage-dependent uptake.
Transporters for the biogenic amines dopamine, norepinephrine, epinephrine and serotonin are largely responsible for transmitter inactivation after release. They also serve as high-affinity targets for a number of clinically relevant psychoactive agents, including antidepressants, cocaine, and amphetamines. Despite their prominent role in neurotransmitter inactivation and drug responses, we lack a clear understanding of the permeation pathway or regulation mechanisms at the single transporter level. The resolution of radiotracer-based flux techniques limits the opportunities to dissect these problems. Here we combine patch-clamp recording techniques with microamperometry to record the transporter-mediated flux of norepinephrine across isolated membrane patches. These data reveal voltage-dependent norepinephrine flux that correlates temporally with antidepressant-sensitive transporter currents in the same patch. Furthermore, we resolve unitary flux events linked with bursts of transporter channel openings. These findings indicate that norepinephrine transporters are capable of transporting neurotransmitter across the membrane in discrete shots containing hundreds of molecules. Amperometry is used widely to study neurotransmitter distribution and kinetics in the nervous system and to detect transmitter release during vesicular exocytosis. Of interest regarding the present application is the use of amperometry on inside-out patches with synchronous recording of flux and current. Thus, our results further demonstrate a powerful method to assess transporter function and regulation.
Galli
A
A
Department of Pharmacology and Center for Molecular Neuroscience, Vanderbilt University Medical Center, Nashville TN 37232-6600, USA.
Blakely
R D
RD
DeFelice
L J
LJ
D002352:Carrier Proteins / Q000502:physiology*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D004594:Electrophysiology / Q000379:methods; D006801:Humans; D007473:Ion Channels / Q000502:physiology*; D007668:Kidney; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008954:Models, Biological; D008961:Models, Structural; D009638:Norepinephrine / Q000378:metabolism* / Q000494:pharmacology; D050484:Norepinephrine Plasma Membrane Transport Proteins; D018408:Patch-Clamp Techniques; D011994:Recombinant Proteins / Q000378:metabolism; D027981:Symporters; D014162:Transfection
D002352:Carrier Proteins; D007473:Ion Channels; D050484:Norepinephrine Plasma Membrane Transport Proteins; D011994:Recombinant Proteins; C493227:SLC6A2 protein, human; D027981:Symporters; D009638:Norepinephrine