title: Regulation of fucosyltransferase-VII expression in peripheral lymph node high endothelial venules.
abstract: Binding of L-selectin to the highly glycosylated peripheral lymph node addressins (PNAd) plays a central role in the normal recirculation of lymphocytes between the bloodstream and the lymph node. This interaction requires correct fucosylation of the PNAd, mediated by the recently identified fucosyltransferase-VII (Fuc-TVII). Here we show that during ontogeny Fuc-TVII is absent at the day of birth, barely detectable on day 1, and clearly present from day 2 onwards. PNAd expression as detected by the MECA-79 antibody precedes the expression of Fuc-TVII. Furthermore, we demonstrate that in adult mice antigenic stimulation of peripheral lymph nodes leads to a temporary disappearance of Fuc-TVII at days 2 and 3 after stimulation, followed by a complete reappearance by day 4, while expression of MECA-79 is never completely absent during this period. Finally, occlusion of afferent lymphatics to peripheral lymph nodes resulted in a decreased expression of Fuc-TVII in the high endothelial venules by day 5, and complete disappearance within 8 days. We conclude that the activity of Fuc-TVII in cells of high endothelial venules is directly affected by afferent lymph and activation processes that occur in the lymph node after antigenic stimulation. The expression of Fuc-TVII is therefore yet another level at which the function of high endothelial venules, and thus lymphocyte trafficking, can be regulated.
authors.affiliation: Department of Cell Biology and Immunology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
authors.forename: V V
authors.initials: VV
authors.lastname: Swarte
authors.forename: D H
authors.initials: DH
authors.lastname: Joziasse
authors.forename: D H
authors.initials: DH
authors.lastname: Van den Eijnden
authors.forename: B
authors.initials: B
authors.lastname: Petryniak
authors.forename: J B
authors.initials: JB
authors.lastname: Lowe
authors.forename: G
authors.initials: G
authors.lastname: Kraal
authors.forename: R E
authors.initials: RE
authors.lastname: Mebius
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000954:Antigens, Surface / Q000096:biosynthesis; D015815:Cell Adhesion Molecules / Q000276:immunology; D004730:Endothelium, Vascular / Q000201:enzymology*; D005647:Fucosyltransferases / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008198:Lymph Nodes / Q000196:embryology / Q000201:enzymology*; D008565:Membrane Proteins; D051379:Mice; D008807:Mice, Inbred BALB C; D010081:Oxazolone / Q000494:pharmacology
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D015815:Cell Adhesion Molecules; C069886:L-selectin counter-receptors; C091520:MECA-325 antigen, mouse; D008565:Membrane Proteins; D010081:Oxazolone; D005647:Fucosyltransferases; C031692:galactoside 3-fucosyltransferase