Acute hyperinsulinemia fails to change GLUT-4 content in crude membranes from goat skeletal muscles and adipose tissue.
The effect of insulin on GLUT-4 protein level in samples of adipose tissue and skeletal muscles from goats was studied in vivo using an euglycemic hyperinsulinemic clamp. The clamp was maintained in conscious goats for 6 h in the presence of amino acids to prevent insulin-induced hypoaminoacidemia. GLUT-4 protein was assessed in crude membrane preparations from adipose tissue and four skeletal muscles (longissimus dorsi, tensor fasciae latae, anconeus and diaphragm) by Western blot analysis. No changes of GLUT-4 protein content were detected after 6 h of hyperinsulinemia in either adipose tissue or skeletal muscles from goats. These results suggest that insulin is not the prime factor involved in the short-term regulation of GLUT-4 protein transporter content in insulin-sensitive tissues from goats.
Balage
M
M
Unité d'Etude du Métabolisme Azoté, INRA-Centre de Clermont Ferrand, Theix, Saint Genès Champanelle, France. balage@clermont.inra.fr
Larbaud
D
D
Debras
E
E
Hocquette
J F
JF
Grizard
J
J
D000208:Acute Disease; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation; D005943:Gluconeogenesis; D051275:Glucose Transporter Type 4; D006041:Goats; D006946:Hyperinsulinism / Q000378:metabolism*; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*
D051275:Glucose Transporter Type 4; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins