title: [Detection of poliovirus by digoxigenin-labeled cDNA probe prepared by PCR technique].
abstract: A study including reverse transcribed cDNAs of Sabin I, II, III virus used as templates and digoxigenin-labeled dUTP added to the reaction solution was carried out. After amplification, digoxigenin-labeled poliovirus cDNA probe was prepared by this PCR technique. Results showed that the direct preparation of digoxigenin-labeled poliovirus cDNA probe by PCR is a convenient and rapid method with a high labeling rate. Compared with neutralization test, the probe has the advantages of more rapid, more sensitive and more specific for poliovirus typing.
authors.affiliation: Guangxi Health and Anti-epidemic Center, Nanning.
authors.forename: M T
authors.initials: MT
authors.lastname: Liu
authors.forename: S S
authors.initials: SS
authors.lastname: Wang
authors.forename: T F
authors.initials: TF
authors.lastname: Zhu
mesh_terms: D018076:DNA, Complementary; D004279:DNA, Viral / Q000235:genetics; D004076:Digoxigenin; D006801:Humans; D017955:Poliovirus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D004076:Digoxigenin