The effect of leustroducsin B on the production of cytokines by human mesenchymal cells.
Leustroducsin B (LSN-B), a novel colony-stimulating factor (CSF) inducer, has been shown to have various biologic activities in vivo. To compare the CSF-inducing activity of LSN-B in vitro with that of the well-known cytokine inducer, interleukin-1beta (IL-1beta), bacterial lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA), we measured granulocyte (G)-CSF and granulocyte-macrophage (GM)-CSF levels that were induced with the stimuli in several mesenchymal cells. The results indicated that each stimulant displayed a different profile in the induction of G-CSF and GM-CSF. Next, to investigate if LSN-B induces cytokines other than G-CSF and GM-CSF, we characterized cytokines that were induced with LSN-B from bone marrow stromal cells (BMSCs). The results showed that a variety of cytokines, including G-CSF and GM-CSF, were induced in both clonal and primary BMSCs. From these results, we speculate that LSN-B induces cytokine production via a regulatory pathway distinct from that of IL-1beta, LPS, or PMA and that this signaling of LSN-B might lead to the production of a variety of cytokines in BMSCs. In addition, from our in vitro and in vivo results, we speculate that the biologic activities of LSN-B in vivo might be based on its own cytokine-inducing activity even though the target cell type of LSN-B in vivo remains to be determined.
Koishi
R
R
Biomedical Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan. koishi@shina.sankyo.co.jp
Serizawa
N
N
Kohama
T
T
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D016179:Granulocyte Colony-Stimulating Factor / Q000096:biosynthesis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000096:biosynthesis; D006801:Humans; D007783:Lactones / Q000494:pharmacology; D008648:Mesoderm / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011753:Pyrones; D015398:Signal Transduction / Q000187:drug effects
D000900:Anti-Bacterial Agents; D016207:Cytokines; D007783:Lactones; D009943:Organophosphorus Compounds; D011753:Pyrones; D016179:Granulocyte Colony-Stimulating Factor; C084414:leustroducsin B; D016178:Granulocyte-Macrophage Colony-Stimulating Factor