Human but not ovine isolates of Bordetella parapertussis are highly clonal as determined by PCR-based RAPD fingerprinting.
The DNA fingerprints of 170 human isolates and ten ovine isolates of Bordetella parapertussis were examined by arbitrarily-primed PCR/RAPD with 29 primers. Based on this technique, all the human isolates appear highly genetically homogeneous. The ovine isolates could be distinguished from human isolates and they showed diversity among themselves. Therefore, human isolates of B. parapertussis are a highly clonal group adapted to infect humans and they are distinct from polymorphic ovine isolates.
Yuk
M H
MH
Dept. of Microbiology and Immunology, UCLA School of Medicine 90095-1747, USA.
Heininger
U
U
Martínez de Tejada
G
G
Miller
J F
JF
D000818:Animals; D001884:Bordetella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001885:Bordetella Infections / Q000382:microbiology* / Q000662:veterinary; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D006801:Humans; D009520:New Zealand; D019105:Random Amplified Polymorphic DNA Technique; D012606:Scotland; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*; D013045:Species Specificity
D004269:DNA, Bacterial