[Genetic subtyping of HIV-1 strains by heteroduplex mobility assay].
DNA fragments of HIV-1 env gene were amplified by nested PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from 24 HIV-1 infected individuals. The PCR products were separated by melting and annealing with denatured PCR product prepared from reference plasimd of representative subtypes. Heteroduplex were then formed between the single-stranded DNA from the two sources and were analysed on polyacrylamide gels. The results from heteroduplex mobility assay (HMA) were compared with HIV-1 subtype results determined by DNA sequencing. With advantages of high speed, low cost and high specificity, HMA is a reliable screening method for HIV-1 subtyping.
Yu
H
H
Institute of Virology, CAPM, Beijing.
Su
L
L
Shao
J J
JJ
D000163:Acquired Immunodeficiency Syndrome / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D015752:Genes, env / Q000235:genetics*; D015497:HIV-1 / Q000145:classification / Q000235:genetics*; D006801:Humans; D009692:Nucleic Acid Heteroduplexes; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA
D004279:DNA, Viral; D009692:Nucleic Acid Heteroduplexes