[The effect of substance P upon middle ear effusion of secretory otitis media and the mode of action].
This experiment aimed to explore the effects of substance P (SP) on secretory otitis media (SOM). Both immunohistochemistry ABC-GDN and image pattern analysis technique were adopted to investigate the relation between SP content of SOM middle ear mucosa and middle ear effusion, and observe the effect of SP receptor antagonist spantide and histamine H2 receptor blocker cimetidin on middle ear effusion. The findings showed: middle ear mucosa SP content tended to increase, and had positive correlation with middle ear effusion, intra-abdominally injecting 1 mg/ml Spantide and 1 mg/ml Cinetidin per day could have middle ear effusion decreased obviously, but the quantities of reduction were more significant. The results suggest that SP plays a role in SOM, might accelerate vasodilation and increase the permeability of cupillary in middle ear mucosa mediated by histamine.
Huang
W
W
Department of Otolaryngology, Xijing Hospital, Fourth Military Medical University, Xi'an.
Jiang
H
H
Qiu
J
J
D000818:Animals; D002415:Cats; D002927:Cimetidine / Q000494:pharmacology; D004432:Ear, Middle / Q000378:metabolism*; D006635:Histamine H2 Antagonists / Q000494:pharmacology; D009092:Mucous Membrane / Q000378:metabolism; D010034:Otitis Media with Effusion / Q000378:metabolism*; D018040:Receptors, Neurokinin-1 / Q000187:drug effects; D013373:Substance P / Q000031:analogs & derivatives / Q000378:metabolism* / Q000494:pharmacology
D006635:Histamine H2 Antagonists; D018040:Receptors, Neurokinin-1; D013373:Substance P; D002927:Cimetidine; C042867:spantide