title: A novel variant of translation elongation factor-1beta: isolation and characterization of the rice gene encoding EF-1beta2.
abstract: A rice gene encoding a novel isoform of translation elongation factor-1beta subunit (termed EF-1beta2) was isolated and characterized. The gene comprises of eight exons, and encodes a 226-amino-acid protein. Expression of EF-1beta2 mRNA is abundant in seeds and cultured cells, but is considerably low in the tissues of the rice seedling. Antiserum raised against an EF-1beta2 synthetic peptide detected a protein with a relative molecular mass of about 32 kDa, indicating the EF-1beta2 gene is actually expressed in rice tissues. EF-1beta2 showed a close similarity to the cognate subunits from plant (beta and beta').
authors.affiliation: Cryobiosystem Research Center, Faculty of Agriculture, Iwate University, Ueda, Morioka, Iwate 020-8550, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Terui
authors.forename: K
authors.initials: K
authors.lastname: Tsutsumi
authors.forename: S
authors.initials: S
authors.lastname: Kidou
authors.forename: T
authors.initials: T
authors.lastname: Sawazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Kuroiwa
authors.forename: M
authors.initials: M
authors.lastname: Yamaki
authors.forename: S
authors.initials: S
authors.lastname: Ejiri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000235:genetics; D017343:Genes, Plant; D014644:Genetic Variation; D015698:Genomic Library; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012275:Oryza / Q000235:genetics*; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
chemical_list: D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors