title: Partitioning of rhodanese onto GroEL. Chaperonin binds a reversibly oxidized form derived from the native protein.
abstract: The mammalian mitochondrial enzyme, rhodanese, can form stable complexes with the Escherichia coli chaperonin GroEL if it is either refolded from 8 M urea in the presence of chaperonin or is simply added to the chaperonin as the folded conformer at 37 degreesC. In the presence of GroEL, the kinetic profile of the inactivation of native rhodanese followed a single exponential decay. Initially, the inactivation rates showed a dependence on the chaperonin concentration but reached a constant maximum value as the GroEL concentration increased. Over the same time period, in the absence of GroEL, native rhodanese showed only a small decline in activity. The addition of a non-denaturing concentration of urea accelerated the inactivation and partitioning of rhodanese onto GroEL. These results suggest that the GroEL chaperonin may facilitate protein unfolding indirectly by interacting with intermediates that exist in equilibrium with native rhodanese. The activity of GroEL-bound rhodanese can be completely recovered upon addition of GroES and ATP. The reactivation kinetics and commitment rates for GroEL-rhodanese complexes prepared from either unfolded or native rhodanese were identical. However, when rhodanese was allowed to inactivate spontaneously in the absence of GroEL, no recovery of activity was observed upon addition of GroEL, GroES, and ATP. Interestingly, the partitioning of rhodanese and its subsequent inactivation did not occur when native rhodanese and GroEL were incubated under anaerobic conditions. Thus, our results strongly suggest that the inactive intermediate that partitions onto GroEL is the reversibly oxidized form of rhodanese.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Smith
authors.forename: P A
authors.initials: PA
authors.lastname: Voziyan
authors.forename: M T
authors.initials: MT
authors.lastname: Fisher
mesh_terms: D018834:Chaperonin 60 / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D017510:Protein Folding; D013884:Thiosulfate Sulfurtransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D013884:Thiosulfate Sulfurtransferase