title: [In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR)].
abstract: It was demonstrated that biologically active ALR could be expressed from its cDNA in transient expression experiments in cos-7 cells. The results showed that the cytosolic fraction from the transfected cells with constructed plasmids DNA stimulated of DNA synthesis of in vitro HTC cells in a dose dependent manner. This finding suggests that the HTC hepatoma cell line may be used as a target for bioassay of human ALR in vitro.
authors.affiliation: Beijing Institute of Radiation Medicine.
authors.forename: X M
authors.initials: XM
authors.lastname: Yang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Hu
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Wu
authors.forename: C T
authors.initials: CT
authors.lastname: Wu
authors.forename: F C
authors.initials: FC
authors.lastname: He
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D010455:Peptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D004273:DNA, Neoplasm; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011994:Recombinant Proteins; C073456:hepatic stimulator substance