Effect of long-term treatment with a purified micronized flavonoid fraction on pancreatic mononuclear cell infiltration in diabetic BB rats.
Bio Breeding (BB) rats develop a genetically determined insulin-dependent diabetes, because of the early destruction of pancreatic beta cells of Langerhans islets, massively infiltrated by inflammatory mononuclear cells. S 5682, registered as Daflon, 500 mg, is a purified micronized flavonoid fraction (90% diosmin, 10% hesperidin), which has been shown to possess antiinflammatory properties, including anti-free radical activity, effects on vascular permeability, venous tone, and perivenous inflammation. We studied the effect of S 5682 on the course of pancreatic insulitis in diabetic BB rats. All the diabetic BB rats were hyperglycemic, with an increase of plasma levels of fructosamine, alpha-1 acid glycoprotein, and fibrinogen, and a dramatic decrease of C-peptide level. These parameters were not modified by S 5682. Pancreas histologic studies showed that in S 5682-treated diabetic BB rats, lymphocytic infiltration of Langerhans islets was less important and frequent than in untreated diabetic BB rats. By quantitative analysis, a highly significant difference was observed for insulitis, as well as perivasculitis, between S 5682-treated and untreated diabetic BB rats. This inhibitory effect of S 5682 on pancreatic mononuclear cell infiltration may be useful for a complementary treatment to decrease the development of insulitis in human insulin-dependent diabetes mellitus.
Guillot
R
R
Equipe de Recherche sur la Biochimie et la Pharmacologie des Vaisseaux et du Rein, Faculté de Médecine, Broussais-Hôtel-Dieu, Université de Paris V, France.
Ogneva
V
V
Hadjiisky
P
P
Kassab
J P
JP
Andre
J
J
Mozère
G
G
Peyroux
J
J
Sternberg
M
M
D000818:Animals; D002096:C-Peptide / Q000097:blood; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000473:pathology*; D004145:Diosmin / Q000494:pharmacology*; D004338:Drug Combinations; D005340:Fibrinogen / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D019270:Fructosamine / Q000378:metabolism; D006569:Hesperidin / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000473:pathology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D008297:Male; D009961:Orosomucoid / Q000378:metabolism; D051381:Rats; D011913:Rats, Inbred BB
D002096:C-Peptide; D004338:Drug Combinations; D005419:Flavonoids; D009961:Orosomucoid; C080675:S 5682; D019270:Fructosamine; D004145:Diosmin; D005340:Fibrinogen; D006569:Hesperidin