title: Ammonium/methylammonium permeases of a Cyanobacterium. Identification and analysis of three nitrogen-regulated amt genes in synechocystis sp. PCC 6803.
abstract: Ammonium is an important nitrogen source for many microorganisms and plants. Ammonium transporters whose activity can be probed with [14C]methylammonium have been described in several organisms including some cyanobacteria, and amt genes encoding ammonium/methylammonium permeases have been recently identified in yeast, Arabidopsis thaliana, and some bacteria. The unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibited a [14C]methylammonium uptake activity that was inhibited by externally added ammonium. Three putative amt genes that are found in the recently published complete sequence of the chromosome of strain PCC 6803 were inactivated by insertion of antibiotic resistance-encoding gene-cassettes. The corresponding mutant strains were impaired in uptake of [14C]methylammonium. Open reading frame sll0108 (amt1) was responsible for a high affinity uptake activity (Ks for methylammonium, 2.7 microM), whereas open reading frames sll1017 (amt2) and sll0537 (amt3) made minor contributions to uptake at low substrate concentrations. Expression of the three amt genes was higher in nitrogen-starved cells than in cells incubated in the presence of a source of nitrogen (either ammonium or nitrate), but amt1 was expressed at higher levels than the other two amt genes. Transcription of amt1 was found to take place from a promoter bearing the structure of the cyanobacterial promoters activated by the nitrogen control transcription factor, NtcA.
authors.affiliation: Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, E-41092 Sevilla, Spain.
authors.forename: M L
authors.initials: ML
authors.lastname: Montesinos
authors.forename: A M
authors.initials: AM
authors.lastname: Muro-Pastor
authors.forename: A
authors.initials: A
authors.lastname: Herrero
authors.forename: E
authors.initials: E
authors.lastname: Flores
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008565:Membrane Proteins; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008744:Methylamines / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000172:deficiency; D016366:Open Reading Frames; D010940:Plant Proteins; D011401:Promoter Regions, Genetic; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D036261:Receptor, EphB6; D017386:Sequence Homology, Amino Acid
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008744:Methylamines; D009566:Nitrates; D010940:Plant Proteins; D000644:Quaternary Ammonium Compounds; D036261:Receptor, EphB6; C088952:ammonium transporters, plant; C027451:methylamine; D009584:Nitrogen