Establishment of monoclonal antibody, gp21-34, against HTLV-II envelope protein (p20E).
A monoclonal antibody (MAb), gp21-34, specifically reactive with human T-lymphotropic virus type-II (HTLV-II) transmembranous envelope glycoprotein (p20E) was developed by immunization with a recombinant protein fused with thioredoxin moiety at the N-terminal portion. This MAb, which belongs to the IgG1 kappa subclass, reacted with HTLV-II infected cell lines (TON-1, C3-44, and Si-IIA) by IFA, but not with HTLV-I infected cell lines (TCL-Kan and MT-2). By Western blot analysis, this MAb reacted with p20E of HTLV-II lysates but not with HTLV-I lysates. Some epitope analyses with synthetic peptides were carried out to look for a plausible linear epitope in the C-terminal region of p20E.
Miyagawa
E
E
Ube Research Laboratory, Fujirebio, Inc., Yoshiwa Ube, Yamaguchi, Japan.
Yoshiki
A
A
Fujii
N
N
Honda
H
H
Ueno
E
E
Kurano
Y
Y
Ito
S
S
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015480:Deltaretrovirus Antibodies; D000939:Epitopes; D015367:Human T-lymphotropic virus 2 / Q000276:immunology*; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology; D011993:Recombinant Fusion Proteins / Q000276:immunology; D015682:Retroviridae Proteins, Oncogenic / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D015480:Deltaretrovirus Antibodies; D000939:Epitopes; D009842:Oligopeptides; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D015682:Retroviridae Proteins, Oncogenic; D014759:Viral Envelope Proteins; C116194:p20E protein, human T-cell leukemia virus type II