Developmental potential of bovine embryos reconstructed from enucleated matured oocytes fused with cultured somatic cells.
Muscle and skin biopsies taken from bovine fetuses and young calves have been used as a source of donor nuclei for cloning experiments. After culture, cells were individually fused to enucleated matured oocytes and the resulting blastocysts obtained after 7 d of culture (3-8% depending on the cell type) were transferred to foster recipient heifers. Two calves, a female and a male, both originating from muscle cells were born, and four additional pregnancies have surpassed mild-term gestation. The pregnancies include one fetus established from a transgenic nucleus from a fetal skin cell, and another one resulting from a skin biopsy performed on a female calf. Our data demonstrate that nuclei from cultured bovine somatic cells obtained from differentiated tissues can be made multipotent.
Vignon
X
X
Unité de biologie cellulaire et microscopie, Jouy-en-Josas, France.
Chesné
P
P
Le Bourhis
D
D
Fléchon
J E
JE
Heyman
Y
Y
Renard
J P
JP
D000818:Animals; D001706:Biopsy; D002417:Cattle; D002459:Cell Fusion / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000502:physiology; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D008297:Male; D009132:Muscles / Q000473:pathology; D009865:Oocytes / Q000502:physiology* / Q000648:ultrastructure; D011247:Pregnancy; D011256:Pregnancy Outcome; D012867:Skin / Q000473:pathology