Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences.
Hagiwara
K
K
Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
Freeman
A A
AA
McMenamin
M G
MG
Harris
C C
CC
D001419:Bacteria; D003001:Cloning, Molecular / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D008970:Molecular Weight; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D051901:Smad4 Protein; D015534:Trans-Activators / Q000235:genetics
D017931:DNA Primers; D004268:DNA-Binding Proteins; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D015534:Trans-Activators; D004262:DNA Restriction Enzymes