Recovery of microvascular blood perfusion and energy metabolism of the obstructed rabbit urinary bladder after relieving outlet obstruction.
We investigated the changes in urinary bladder microvascular blood perfusion and energy metabolism following outlet obstruction and after relieving the obstruction. We induced mild bladder outlet obstruction in male New Zealand White rabbits. Following 2 weeks of obstruction, one group of animals (n = 6) was sacrificed, while outlet obstruction was relieved in three additional groups, which were sacrificed 1 (n = 5), 2 (n = 5) and 4 (n = 5) weeks after relieving the obstruction. Seven sham-operated rabbits served as controls. Before obstruction, immediately before relieving the obstruction and preceding the sacrifice, the microvascular blood perfusion of the urinary bladder was measured using a laser Doppler blood flowmeter. The detrusor content of phosphocreatine and adenine nucleotides was determined by high-performance liquid chromatography. The results showed: (1) 2 weeks of outlet obstruction significantly decreased the bladder microvascular blood perfusion, which recovered gradually after relief of the obstruction and returned to the control level by 4 weeks of obstruction reversal; (2) outlet obstruction reduced detrusor energy charge and phosphocreatine content, which were restored in parallel after relieving the obstruction; by 4 weeks the bladder had regained their normal energy producing capability; (3) bladder microvascular perfusion has a very close correlation with detrusor energy charge (r = 0.791, p < 0.001). In conclusion, our findings of the close correlation between microvascular perfusion and energy production in bladder outlet obstruction suggest an important role for the decreased microvascular blood perfusion in reducing bladder energy production.
Tong-Long Lin
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Division of Urology, Department of Surgery, Veterans General Hospital-Taipei and National Yang-Ming University School of Medicine, Taipei, Taiwan. lintl@vghtpe.gov.tw
Chen
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Yang
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Chang
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LS
D000227:Adenine Nucleotides / Q000378:metabolism*; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D004734:Energy Metabolism; D017078:Laser-Doppler Flowmetry; D008297:Male; D008833:Microcirculation; D009929:Organ Size; D010477:Perfusion; D010725:Phosphocreatine / Q000378:metabolism*; D011817:Rabbits; D012016:Reference Values; D012039:Regional Blood Flow / Q000502:physiology; D001743:Urinary Bladder / Q000098:blood supply* / Q000473:pathology; D001748:Urinary Bladder Neck Obstruction / Q000503:physiopathology* / Q000601:surgery*
D000227:Adenine Nucleotides; D010725:Phosphocreatine