Elevated levels of Mr 92,000 type IV collagenase during tumor growth in vivo.
Our previous studies demonstrated that matrix metalloproteinase (MMP-9) levels were significantly higher in human glioblastoma tissue samples than in low-grade brain tumors and normal brain tissue (Rao et al., Cancer Res. 53, 2208-2211, 1993). In the present study, we measured the levels of MMP-2 and MMP-9 during the growth of glial tumors in nude mice by intracerebral injection of glioblastoma cells. Using gelatin zymography, densitometry, and an enzyme-linked immunosorbent assay, we found that the enzyme activity and protein count of MMP-2 and MMP-9 were a respective 3- to 10- and 2- to 30-fold higher in tumors at day 14 and 28 than in normal tissue. Immunohistochemical staining for MMP-9 showed strong immunoreactivity in tumor cells and the staining intensity was much higher at day 28, compared to day 14. These results suggest that upregulation of MMP-9 plays a major role in the glioma tumor growth in vivo.
Sawaya
R
R
Department of Neurosurgery, Department of Neuro-Oncology, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas, 77030, USA.
Go
Y
Y
Kyritisis
A P
AP
Uhm
J
J
Venkaiah
B
B
Mohanam
S
S
Gokaslan
Z L
ZL
Rao
J S
JS
D000818:Animals; D002455:Cell Division; D017364:Collagenases / Q000302:isolation & purification / Q000378:metabolism*; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018093:Gelatinases / Q000302:isolation & purification / Q000378:metabolism*; D005909:Glioblastoma / Q000201:enzymology* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D008666:Metalloendopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D051379:Mice; D008819:Mice, Nude; D008970:Molecular Weight; D013997:Time Factors; D014183:Transplantation, Heterologous
D017364:Collagenases; D018093:Gelatinases; D008666:Metalloendopeptidases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9