Effects of the anti-ICAM-1 monoclonal antibody on dextran sodium sulphate-induced colitis in rats.
Increased expression of intercellular adhesion molecule-1 (ICAM-1) in the colon of inflammatory bowel disease (IBD) has been reported. We evaluated the effects of monoclonal antibodies to ICAM-1 on acute colitis induced by dextran sodium sulphate (DSS) in rats. Colitis was induced by feeding rats 3% DSS for 7 days. Anti-ICAM-1 antibody or vehicle alone was injected intraperitoneally in rats daily from day 0 to day 6. On day 7 the rats were killed and colitis was evaluated histologically. Prophylactic treatment with anti-ICAM-1 significantly attenuated colonic damage, neutrophil infiltration and the shortening of the colon in DSS colitis. Our findings demonstrate that ICAM-1 plays an important role in this model of inflammatory bowel disease. Although this study does not directly address the effect of anti-ICAM-1 therapy in IBD, our findings encourage experiments using therapies that target ICAM-1 in rats with already developed disease.
Taniguchi
T
T
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine, Japan.
Tsukada
H
H
Nakamura
H
H
Kodama
M
M
Fukuda
K
K
Saito
T
T
Miyasaka
M
M
Seino
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000627:therapeutic use*; D003093:Colitis, Ulcerative / Q000209:etiology / Q000473:pathology / Q000628:therapy*; D003106:Colon / Q000737:chemistry / Q000473:pathology; D016264:Dextran Sulfate; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis / Q000276:immunology* / Q000502:physiology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000032:analysis; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1; D016264:Dextran Sulfate