title: Immunohistochemical evaluation of cellular deposits on posterior chamber intraocular lenses.
abstract: OBJECTIVE
  We used immunohistochemistry to characterize cellular and proteinaceous deposits on the surfaces of explanted posterior chamber intraocular lenses (PC-IOLs).
  
  
  METHODS
  A total of 30 PC-IOLs were immunostained for the alpha- or beta-subunits of prolyl 4-hydroxylase, which is involved in collagen biosynthesis; cellular fibronectin; alpha B crystalline; and CD68, a macrophage marker, to characterize the cellular deposits that adhere to the IOL surfaces and to evaluate the distribution of cells involved in the deposition of extracellular matrix on IOLs.
  
  
  RESULTS
  Cellular or proteinaceous deposits were observed on all 30 PC-IOLs. Cells that showed positive staining for alpha B crystallin were classified as lens epithelial cells; CD68-positive cells were considered to be of macrophagic origin. Positivity for cellular fibronectin, including the macrophages and related cells, appeared to be responsible for the accumulation of fibronectin on the surfaces of PC-IOLs. Prolyl 4-hydroxylase-positive cells were involved in the deposition of collagen on PC-IOLs.
  
  
  CONCLUSIONS
  Immunohistochemical study revealed that macrophages, foreign-body giant cells, and lens epithelial cells adhered to explanted PC-IOLs. Such adherent cells are responsible for the deposition of extracellular matrix on the surfaces of PC-IOLs and may regulate the assembly of the extracellular matrix, influencing the biocompatibility of PC-IOLs.
authors.affiliation: Department of Ophthalmology, Wakayama Medical College, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Saika
authors.forename: T
authors.initials: T
authors.lastname: Miyamoto
authors.forename: A
authors.initials: A
authors.lastname: Yamanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Kawashima
authors.forename: Y
authors.initials: Y
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Tanaka
authors.forename: O
authors.initials: O
authors.lastname: Yamanaka
authors.forename: S
authors.initials: S
authors.lastname: Ohmi
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: A
authors.initials: A
authors.lastname: Ooshima
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D003094:Collagen / Q000378:metabolism; D003459:Crystallins / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D007910:Lenses, Intraocular; D008264:Macrophages / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D013194:Staining and Labeling; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D003094:Collagen; D003459:Crystallins; D005353:Fibronectins; D011393:Procollagen-Proline Dioxygenase; C067980:CD68 antigen, human; D000097382:CD68 Molecule