In vitro motility disorders associated with displaced abomasum in dairy cows.
The objective of this study was to investigate in vitro abomasal motility in dairy cows diagnosed with displaced abomasum. Longitudinal muscle myenteric plexus preparations originating from the abomasal antrum of control cows, and cows diagnosed with left displaced abomasum (LDA), right displaced abomasum (RDA) or abomasal volvulus (AV) were used. In control preparations electrical field stimulation evoked an immediate cholinergic contractile response exceeding amplitude of basal contractions by 60%. In contrast, contractile activity was significantly inhibited during electrical stimulation in LDA, RDA and AV by 47%, 66% and 45%, respectively. This inhibition was reversed in the presence of L-NAME. The staining intensity of NADPH-positive myenteric neurones was significantly higher in displaced abomasa than in controls. Concentration-response curves indicated that preparations from displaced abomasa showed reduced sensitivity to acetylcholine. This study demonstrated motility disorders in displaced abomasa in vitro. The results suggested that abomasal displacement is associated with malfunctions at the level of the intrinsic nervous system combined with impaired cholinergic muscle responses. There appeared to be a predominance of nitrergic inhibitory mechanisms over excitatory mechanisms. These results might be of significance for diseases associated with gastric hypomotility and emptying disorders.
Geishauser
T
T
Medical and Forensic Veterinary Clinic II (Internal Diseases of Ruminants), Justus-Liebig-University, Giessen, Germany.
Reiche
D
D
Schemann
M
M
D000018:Abomasum / Q000201:enzymology / Q000473:pathology / Q000503:physiopathology*; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000473:pathology / Q000503:physiopathology*; D004558:Electric Stimulation; D005260:Female; D005769:Gastrointestinal Motility / Q000502:physiology*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D009130:Muscle, Smooth / Q000294:innervation / Q000473:pathology / Q000503:physiopathology; D009252:NADPH Dehydrogenase / Q000378:metabolism; D013272:Stomach Diseases / Q000473:pathology / Q000503:physiopathology / Q000662:veterinary*
D009252:NADPH Dehydrogenase; D000109:Acetylcholine