Identification and initial characterization of mSLK, a murine member of the STE20 family of kinases.
Protein kinases play a major role in the regulation of cellular growth. We isolated a murine cDNA encoding a novel serine/threonine kinase (referred to as mSLK) ubiquituously expressed during all stages of murine development and in all adult tissues examined. The cDNA codes for a 1233-amino-acid polypeptide characterized by an N-terminal catalytic domain and a large C-terminal region of unknown function. The sequence of the catalytic domain places mSLK in the STE20 family of cytoplasmic kinases. The noncatalytic domain does not show significant homology to any known protein. mSLK expressed in either COS7 cells or in bacteria was shown to contain kinase activity. The N-terminal fragment of mSLK was able to autophosphorylate on serine and threonine residues, phosphorylate threonine residues on a C-terminal fragment of the molecule, and phosphorylate exogenous substrates myelin basic protein and histone III in vitro. Furthermore, autophosphorylation of the catalytic domain was enhanced in the presence of the C-terminal fragment, which suggests a possible regulatory role for the C-terminal region of mSLK. A genomic clone of mSLK was isolated and used for fluorescence in situ hybridization locating the mSLK gene on band D2 of mouse chromosome 19.
Pytowski
B
B
ImClone Systems Incorporated, New York, New York, 10014, USA. bronek@imclone.com
Hicklin
D J
DJ
Kornhaber
G
G
Dellaratta
D V
DV
Witte
L
L
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D003001:Cloning, Molecular; D047908:Intracellular Signaling Peptides and Proteins; D020930:MAP Kinase Kinase Kinases; D051379:Mice; D008969:Molecular Sequence Data; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; D017346:Protein Serine-Threonine Kinases; D020930:MAP Kinase Kinase Kinases; C078754:STE20 protein, S cerevisiae