Areca nut extract inhibits the growth, attachment, and matrix protein synthesis of cultured human gingival fibroblasts.
Betel quid chewing is a popular oral habit in India, South Africa, and many Southeast Asian countries. The effects of areca nut (AN) extract on the growth, attachment, and protein synthesis of healthy human gingival fibroblasts (GF) were investigated to determine why betel quid (BQ) chewers have higher prevalence of periodontal disease than non-chewers. Twenty-four hour exposure of human GF to AN extract (> 200 microg/ml) in culture led to the formation of numerous intracellular vacuoles. As analyzed by modified MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay, AN extract significantly suppressed the growth of GF over 5 days of incubation in a dose-dependent manner. At concentrations of 50 and 300 microg/ml, AN extract suppressed the growth of GF with 30% and 57% (P < 0.05), respectively. AN extract also significantly suppressed the synthesis of [3H]proline incorporation into trichloroacetic acid (TCA) precipitated proteins. At concentrations of 200, 400, and 600 microg/ml, AN extract suppressed the protein synthesis with 33%, 58%, and 63% of inhibition (P < 0.05), respectively. Preincubation of cells in a medium containing AN extract for 2 hours inhibits the subsequent attachment of cultured GF to type I collagen at the 50% inhibitory concentration (IC50) which is about 720 to 798 microg/ml. Considering the frequent consumption of BQ throughout the day, impairment of sequential fibroblast functions by BQ ingredients is a potential mechanism through which BQ chewing exert a deleterious effect to the gingival tissues.
Chang
M C
MC
Team of Biomedical Science, Chang-Gung Institute of Nursing, Taoyuan, Taiwan.
Kuo
M Y
MY
Hahn
L J
LJ
Hsieh
C C
CC
Lin
S K
SK
Jeng
J H
JH
D001114:Areca / Q000009:adverse effects; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004396:Coloring Agents; D004305:Dose-Response Relationship, Drug; D016326:Extracellular Matrix Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D005881:Gingiva / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D010510:Periodontal Diseases / Q000209:etiology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010946:Plants, Medicinal; D015995:Prevalence; D011392:Proline / Q000378:metabolism; D019275:Radiopharmaceuticals; D013778:Tetrazolium Salts; D013844:Thiazoles; D013997:Time Factors; D014316:Tritium; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure
D004396:Coloring Agents; D016326:Extracellular Matrix Proteins; D010936:Plant Extracts; D019275:Radiopharmaceuticals; D013778:Tetrazolium Salts; D013844:Thiazoles; D014316:Tritium; D003094:Collagen; D011392:Proline; C022616:thiazolyl blue