title: Kinetics of the graft-versus-leukemia response after donor leukocyte infusions for relapsed chronic myeloid leukemia after allogeneic bone marrow transplantation.
abstract: Little is known about the mechanisms and the kinetics of the so-called graft-versus-leukemia (GVL) response induced by donor lymphocyte infusions (DLI) in patients with leukemic relapse after allogeneic bone marrow transplantation (BMT). We sought to elucidate this problem by sequentially studying three patients with relapsed chronic myeloid leukemia after sex-mismatched BMT from time before donor leukocyte infusion until achievement of complete molecular remission. Lineage-specific chimerism was assessed longitudinally by a combined fluorescent immunophenotyping and sex chromosome-specific in situ hybridization approach. Results were related to quantitative detection of bcr-abl transcripts by competitive differential reverse transcriptase-polymerase chain reaction (RT-PCR), qualitative bcr-abl RT-PCR, and multiplex PCR-based DNA donor/recipient chimerism. All patients had predominant donor lymphopoiesis at the time of DLI, suggesting a state of tolerance to recipient leukemic and/or normal cells. In contrast, granulopoiesis and erythropoiesis were mainly recipient derived in both patients with hematologic relapse and partly recipient derived in the patient with molecular relapse. Eighty percent, 90%, and 8% of CD34(+) cells, respectively, were found to be of recipient origin at relapse, and few donor stem cells predicted for cytopenia post-DLI. Responses were seen after a time lag of 5 to 13 weeks after DLI and resulted in reversal to full donor chimerism within a critical switch period of 4 to 5 weeks. A sudden decrease in recipient cells was paralleled by a sharp decrease in bcr-abl transcript numbers detectable several weeks before achievement of molecular remission and onset of clinical graft-versus-host disease (GVHD). This response pattern was confirmed by retrospective RT-PCR analysis in an additional five patients. Prospective monitoring of stem cell chimerism and response may enable us to individually tailor adoptive immunotherapy in the future.
authors.affiliation: Abteilung für Innere Medizin mit Schwerpunkt Hämatologie/Onkologie, Virchow Klinikum der Humboldt Universität zu Berlin, Berlin, Germany. Herrad.Baurmann@t-online.de
authors.forename: H
authors.initials: H
authors.lastname: Baurmann
authors.forename: S
authors.initials: S
authors.lastname: Nagel
authors.forename: T
authors.initials: T
authors.lastname: Binder
authors.forename: A
authors.initials: A
authors.lastname: Neubauer
authors.forename: W
authors.initials: W
authors.lastname: Siegert
authors.forename: D
authors.initials: D
authors.lastname: Huhn
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood; D016026:Bone Marrow Transplantation; D002678:Chimera; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000097:blood; D020350:Graft vs Tumor Effect; D006801:Humans; D016219:Immunotherapy, Adoptive; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000628:therapy; D015466:Leukemia, Myeloid, Chronic-Phase / Q000628:therapy; D017708:Leukocyte Transfusion; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D012008:Recurrence; D012189:Retrospective Studies; D016879:Salvage Therapy; D013997:Time Factors; D014184:Transplantation, Homologous; D017211:Treatment Failure
chemical_list: D014408:Biomarkers, Tumor; D016044:Fusion Proteins, bcr-abl