Overexpression of cation-dependent mannose 6-phosphate receptor prevents cell death induced by serum deprivation in PC12 cells.
PC12 cells express well cation-independent mannose 6-phosphate receptors (CI-MPR), but not cation-dependent (CD)-MPR as much. To examine CD-MPR dependency of transport of cathepsins B and D to lysosomes in PC12 cells, we prepared the cells overexpressing CD-MPR. Immunoreactivity for cathepsin B became more distinct and larger in size in the transfected cells than in wild-type cells. No difference in the distribution of cathepsin D was seen between these two cells. The viability of the cells following serum deprivation was significantly higher in the transfected cells than in wild-type cells. This increased viability of the transfected cells was blocked by CA074, a specific inhibitor of cathepsin B, while pepstatin A suppressed the action of CA074. The results suggest that CD-MPR preferentially transport cathepsin B in PC12 cells, and cathepsins B and D participate in the regulation of PC12 cell apoptosis.
Kanamori
S
S
Department of Conservative Dentistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Waguri
S
S
Shibata
M
M
Isahara
K
K
Ohsawa
Y
Y
Konishi
A
A
Kametaka
S
S
Watanabe
T
T
Ebisu
S
S
Kominami
E
E
Uchiyama
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002401:Cathepsin B / Q000032:analysis; D002402:Cathepsin D / Q000032:analysis; D002412:Cations / Q000378:metabolism*; D002470:Cell Survival; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D016716:PC12 Cells / Q000378:metabolism*; D051381:Rats; D017527:Receptor, IGF Type 2 / Q000096:biosynthesis*
D002412:Cations; D016895:Culture Media, Serum-Free; D017527:Receptor, IGF Type 2; D002401:Cathepsin B; D002402:Cathepsin D