title: Higher-order structure and thermal instability of bovine mitochondrial tRNASerUGA investigated by proton NMR spectroscopy.
abstract: Although mammalian mitochondrial serine-specific tRNA with the anticodon UGA (tRNASerUGA) appears to possess an almost normal cloverleaf secondary structure, it exhibits an extraordinarily low melting temperature (tm). An in vitro tRNASerUGA transcript without modified nucleosides had an even lower tm and slightly less hyperchromicity, but its tertiary structure was apparently very similar to that of the native counterpart judging from its aminoacylation activity and the body of experimental evidence so far obtained for canonical tRNAs. The transcript was therefore used to investigate the higher-order structure and thermal instability of tRNASerUGA. 1H-NMR analysis of the transcript showed that it takes a nearly L-shaped tertiary structure with similar tertiary base-pairings to those found in yeast tRNAPhe, which is representative of canonical tRNAs. However, magnesium ion titration revealed that Mg2+ affected the chemical shifts of the tRNASerUGA transcript differently than those of canonical tRNAs so far studied; the former was less sensitive toward Mg2+, especially in the D-arm region. This observation was confirmed by NMR analysis with paramagnetic manganese ion titration. Hill plots derived from the CD spectral changes caused by titration with Mg2+ suggested that the tRNASerUGA transcript had fewer Mg2+ binding sites than those of yeast tRNAPhe as well as its transcript, a finding that was consistent with the NMR data. We thus surmise that the thermal instability of both the transcript and tRNASerUGA itself originated from a reduction in the number of the divalent ion binding sites within the tRNA molecule. These results suggest a new type of thermal instability for mitochondrial tRNA.
authors.affiliation: Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Tokyo, Bunkyo-ku, 113-8656, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hayashi
authors.forename: G
authors.initials: G
authors.lastname: Kawai
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D020029:Base Pairing; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D003062:Codon; D008274:Magnesium / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008345:Manganese / Q000737:chemistry / Q000494:pharmacology; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry / Q000187:drug effects / Q000235:genetics*; D000077278:RNA, Mitochondrial; D012360:RNA, Transfer, Phe / Q000737:chemistry; D012362:RNA, Transfer, Ser / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D015203:Reproducibility of Results; D013696:Temperature; D014158:Transcription, Genetic; D014316:Tritium
chemical_list: D003062:Codon; D000077278:RNA, Mitochondrial; D012360:RNA, Transfer, Phe; D012362:RNA, Transfer, Ser; D014316:Tritium; D008345:Manganese; D012313:RNA; D008274:Magnesium