title: Mutation of the Angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation.
abstract: The E6-AP ubiquitin ligase (human/mouse gene UBE3A/Ube3a) promotes the degradation of p53 in association with papilloma E6 protein, and maternal deficiency causes human Angelman syndrome (AS). Ube3a is imprinted with silencing of the paternal allele in hippocampus and cerebellum in mice. We found that the phenotype of mice with maternal deficiency (m-/p+) for Ube3a resembles human AS with motor dysfunction, inducible seizures, and a context-dependent learning deficit. Long-term potentiation (LTP) was severely impaired in m-/p+ mice despite normal baseline synaptic transmission and neuroanatomy, indicating that ubiquitination may play a role in mammalian LTP and that LTP may be abnormal in AS. The cytoplasmic abundance of p53 was increased in postmitotic neurons in m-/p+ mice and in AS, providing a potential biochemical basis for the phenotype through failure to ubiquitinate and degrade various effectors.
authors.affiliation: Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Y H
authors.initials: YH
authors.lastname: Jiang
authors.forename: D
authors.initials: D
authors.lastname: Armstrong
authors.forename: U
authors.initials: U
authors.lastname: Albrecht
authors.forename: C M
authors.initials: CM
authors.lastname: Atkins
authors.forename: J L
authors.initials: JL
authors.lastname: Noebels
authors.forename: G
authors.initials: G
authors.lastname: Eichele
authors.forename: J D
authors.initials: JD
authors.lastname: Sweatt
authors.forename: A L
authors.initials: AL
authors.lastname: Beaudet
mesh_terms: D000161:Acoustic Stimulation; D000818:Animals; D001245:Association Learning / Q000502:physiology; D001921:Brain / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism*; D004195:Disease Models, Animal; D007859:Learning Disabilities / Q000235:genetics*; D008025:Ligases / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism; D017774:Long-Term Potentiation / Q000235:genetics*; D051379:Mice; D009043:Motor Activity / Q000502:physiology; D009154:Mutation / Q000235:genetics; D009474:Neurons / Q000378:metabolism; D012640:Seizures / Q000209:etiology / Q000235:genetics; D013569:Synapses / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
chemical_list: D016159:Tumor Suppressor Protein p53; C471639:UBE3A protein, human; D044767:Ubiquitin-Protein Ligases; D008025:Ligases