title: Kidney ectopeptidases in gentamicin and mercuric chloride-induced acute renal failure.
abstract: The clinical use of gentamicin is associated by nephrotoxicity in over 10% of patients. However, the biochemical events responsible for proximal tubular injury and acute renal failure (ARF) are still unclear. In the present study, effects of gentamicin and, comparatively, of mercuric chloride upon rat kidney cortex ectopeptidases were studied. Gentamicin was given i.p., 100 mg/kg daily for up to 6 days, mercuric chloride i.p. once 2.5 mg/kg to rats studied after 3 days. Kidney cortex aminopeptidase N decreased significantly after 3 and 6 days' treatment (p < 0.01), however, angiotensinase A significantly decreased after 3 (p < 0.01), and much more after 6 days of gentamicin treatment (p < 0.001). Dipeptidylpeptidase IV activity was significantly decreased only after 6 days of treatment with gentamicin. In mercuric chloride-induced ARF all three peptidase activities were markedly decreased (p < 0.001). These results suggest that gentamicin-induced decrease in ectopeptidase activities could compromise vasoactive and other biologically active peptides handling, with important consequences upon renal function and metabolism.
authors.affiliation: Institute of Nephrology and Hemodialysis, University School of Medicine, Nis, Yugoslavia.
authors.forename: V
authors.initials: V
authors.lastname: Stefanović
authors.forename: P
authors.initials: P
authors.lastname: Vlahović
authors.forename: V
authors.initials: V
authors.lastname: Savić
authors.forename: S
authors.initials: S
authors.lastname: Ilić
authors.forename: M
authors.initials: M
authors.lastname: Mitić-Zlatković
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced*; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D018826:CD13 Antigens / Q000378:metabolism; D003404:Creatinine / Q000097:blood; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D020689:Exopeptidases; D005839:Gentamicins / Q000633:toxicity*; D043384:Glutamyl Aminopeptidase; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008627:Mercuric Chloride / Q000633:toxicity*; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014508:Urea / Q000097:blood
chemical_list: D005839:Gentamicins; D008627:Mercuric Chloride; D014508:Urea; D003404:Creatinine; D020689:Exopeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D018826:CD13 Antigens; D043384:Glutamyl Aminopeptidase; D018819:Dipeptidyl Peptidase 4