title: [26S-ribonucleoprotein complex (26S-proteasome) directly interacts with fibrillar actin].
abstract: 26S-complex was purified from rat liver cells by centrifugation in sucrose gradients and ion exchange chromatography. The ability of 26S-proteasome to interact with fibrillar actin (F-actin) was examined by rapid cosidementation of these particles with F-actin in isokinetic sucrose gradients. It was shown that direct interaction occurred only at a low ATP concentration.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
authors.forename: V E
authors.initials: VE
authors.lastname: Galkin
authors.forename: L V
authors.initials: LV
authors.lastname: Turoverova
authors.forename: I M
authors.initials: IM
authors.lastname: Konstantinova
authors.forename: G P
authors.initials: GP
authors.lastname: Pinaev
mesh_terms: D000199:Actins / Q000737:chemistry*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D015151:Immunoblotting; D008099:Liver / Q000737:chemistry*; D008297:Male; D010447:Peptide Hydrolases / Q000737:chemistry*; D046988:Proteasome Endopeptidase Complex; D051381:Rats
chemical_list: D000199:Actins; D010447:Peptide Hydrolases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease