Potential use of repeated fluorescence in situ hybridization in the same human blastomeres for preimplantation genetic diagnosis.
OBJECTIVE
To assess the feasibility of repeated fluorescence in situ hybridization (FISH) procedures in the same nucleus of a human blastomere.


METHODS
Three consecutive FISH procedures were performed in the same human blastomere by using direct label fluorescence CEP and WCP probes (Vysis).


METHODS
Hospital-based private IVF program.


METHODS
Twenty-eight infertile couples who underwent conventional IVF in our center.


METHODS
Embryos from oocytes with three pronuclei after in vitro insemination were used in this study.


METHODS
The rates of nuclear loss, present signals, and absent signal were examined.


RESULTS
In group 1, the rates of presence of signals were 94% after the first FISH, 92% after the second FISH, and 88% after the third FISH. In group 2, the rates of presence of signals were 96% after the first FISH, 93% after the second FISH, and 87% after the third FISH. There was no statistically significant difference in the rates of nuclear loss, present signals, and absent signal between three consecutive FISH procedures and between CEP and WCP probes.


CONCLUSIONS
Six or more chromosomes of a single blastomere may be examined with use of this repeated FISH procedure, which may be important for preimplantation genetic diagnosis.
Liu
J
J
The Greater Baltimore Medical Center Fertility Center, Maryland 21204, USA. jliu@gbmc.org
Tsai
Y L
YL
Zheng
X Z
XZ
Baramki
T A
TA
Yazigi
R A
RA
Katz
E
E
D001757:Blastomeres; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*