Expression analysis of the autolysin gene (atl) of Staphylococcus aureus.
The bifunctional autolysin gene (atl) of Staphylococcus aureus was transcribed into a 4.1-kb transcript. The transcription initiation site was located at an adenine residue 33-nt upstream from the putative atl start codon. Analysis using a promoter-reporter plasmid showed that promoter activity increased during the exponential growth phase. The Tn551 insertion site of the autolysis-deficient mutant S. aureus RUSAL2 was located in the putative catalytic region of the glucosaminidase domain.
Oshida
T
T
Lead Generation Research Laboratory, Tanabe Seiyaku Co., Ltd., Toda, Saitama, Japan. oshidata@genox.co.jp
Takano
M
M
Sugai
M
M
Suginaka
H
H
Matsushita
T
T
D001483:Base Sequence; D015152:Blotting, Northern; D004251:DNA Transposable Elements; D005798:Genes, Bacterial / Q000235:genetics*; D017930:Genes, Reporter; D006596:Hexosaminidases / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D013211:Staphylococcus aureus / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D012333:RNA, Messenger; D006596:Hexosaminidases; D009238:N-Acetylmuramoyl-L-alanine Amidase