[Diet and antioxidant--a nutritional intervention study].
It has been implicated that high vegetable intake is associated with a decreased risk for various forms of cancer in epidemiological studies. A major defense system against cancer has shown to be antioxidants. Glutathione S-transferases (GSTs), one of the major defense ststems against oxidative damage, can be induced by antioxidant and addition of vegetables to their normal diet. Serum retinol and a-tocopherol were determined by HPLC and the gene expression of GST pi in lymphocytes was measured by reverse transcription-polymerase chain reaction (RT-PCR) techniques. The results showed that there was on significant difference in the levels of serum retinol and a-tocopherol before and after the addition of vegetables. GST pi mRNA levels in lymphocytes were higher in four out of five subjects after addition of vegetables. The results suggested that it might be other antioxidants than vitamin A and E in the vegetables that induced the gene transcription of GST pi.
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Institute for Chemical Carcinogenesis, Guangzhou Medical College, PR. China.
Persson
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Rylander
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D000328:Adult; D000975:Antioxidants; D004032:Diet; D015870:Gene Expression; D005982:Glutathione Transferase / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000096:biosynthesis; D014675:Vegetables; D014801:Vitamin A / Q000097:blood*; D014810:Vitamin E / Q000097:blood*
D000975:Antioxidants; D012333:RNA, Messenger; D014801:Vitamin A; D014810:Vitamin E; D005982:Glutathione Transferase