Role of ergosterol in growth inhibition of Saccharomyces cerevisiae by syringomycin E.
The antifungal activity of the lipodepsipeptide syringomycin E from Pseudomonas syringae pv. syringae is modulated by sterols. To study the requirement of the predominant fungal sterol, ergosterol, in syringomycin E action, the sterol composition of Saccharomyces cerevisiae sterol auxotroph strain FY-14 was modified and sensitivity to syringomycin E examined. Cells containing solely ergosterol, cholesterol, beta-sitosterol or stigmasterol were sensitive to syringomycin E with the latter two being the most sensitive. Cells containing growth-promoting cholesterol were the most sensitive and those with growth-promoting ergosterol the least sensitive. It is concluded that sensitivity to syringomycin E is modulated by growth-promoting sterols and does not necessarily require ergosterol.
Wangspa
R
R
Department of Biology, Utah State University, Logan 84322-5305, USA.
Takemoto
J Y
JY
D000935:Antifungal Agents / Q000494:pharmacology*; D002855:Chromatography, Thin Layer; D004875:Ergosterol / Q000032:analysis / Q000494:pharmacology*; D010456:Peptides, Cyclic / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development; D013261:Sterols / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology
D000935:Antifungal Agents; D010456:Peptides, Cyclic; D013261:Sterols; C105246:syringomycin E; D004875:Ergosterol