title: [Effects of basic fibroblast growth factor on anoxia/reoxygenation injury of rat neonatal cardiomyocytes].
abstract: The effects of basic fibroblast growth factor (bFGF) on anoxia/reoxygenation (A/R) injury and protein kinase C (PKC) activity were studied on a model of A/R injury of neonatal rat cardiomyocytes to investigate the possibility of its using as a substrate for pharmacological preconditioning. The data indicated that bFGF improved the viability of cardiomyocytes, lowered the deplection of ATP and leakage of intracellular lactate dehydrogenase (LDH) in a concentration-dependent manner. PKC inhibitor, H7, completely abolished the protective effects. It was also found that bFGF directely activated PKC in cardiomyocytes in a time course similar to that in hypoxic preconditioning. The data suggested that the protective effect of bFGF on cardiomyocyte A/R injury might be mediated by PKC.
authors.affiliation: Institute of Cardiovascular Research, Beijing Medical University.
authors.forename: X H
authors.initials: XH
authors.lastname: Liu
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Pang
authors.forename: C S
authors.initials: CS
authors.lastname: Tang
authors.forename: J Y
authors.initials: JY
authors.lastname: Su
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Qin
authors.forename: H
authors.initials: H
authors.lastname: Piao
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D019157:Ischemic Preconditioning, Myocardial; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000166:cytology*; D011493:Protein Kinase C / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D016222:Fibroblast Growth Factor 2; D011493:Protein Kinase C