Numerical abnormalities of chromosomes 1 and 10 in endometrial adenocarcinoma: fluorescence in situ hybridization analysis of 23 archival paraffin-embedded samples.
Retrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei isolated from formalin-fixed paraffin-embedded tissue. We examined 23 archival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosomes 1 and 10 were detected in 39% of the case analyzed, and the frequency of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involving chromosomes 1 and 10 with endometrial carcinoma, as reported by other studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation.
Muresu
R
R
Istituto di Genetica Molecolare del CNR, Alghero, Italy.
Cossu
A
A
Scarpa
A M
AM
Volpi
E V
EV
Rocca
P C
PC
Pintus
A
A
Tibiletti
M G
MG
Tanda
F
F
Pirastu
M
M
Massarelli
G
G
D000328:Adult; D000368:Aged; D000782:Aneuploidy; D018196:Carcinoma, Adenosquamous / Q000235:genetics*; D018269:Carcinoma, Endometrioid / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D016612:Paraffin Embedding; D012189:Retrospective Studies
D005819:Genetic Markers