Reduced mucosal perianastomotic capillary density in rat small intestine with chronic radiation damage.
Anastomoses in an intestine with chronic radiation damage are prone to leakage, possibly due to a reduced blood supply induced by a reduced capillary bed. In an animal model, the numerical capillary density in the perianastomotic area was investigated in intestine with or without chronic radiation damage. A 2-cm segment of rat ileum received a single dose of 21 Gy. Twenty weeks later, when the chronic radiation-induced changes were established, an anastomosis was constructed in this segment and in a corresponding segment in control rats. In situ perfusion fixation of the intestine was done 4 or 7 days after construction of the anastomosis, sections of the intestine were removed surgically, the specimens were embedded in methacrylate plastic and sectioned at 2.5 microm, and capillaries were counted under a light microscope. The circumferential mucosal capillary density was lower in irradiated than in nonirradiated animals at both 4 and 7 days (P < 0.001 and P = 0.04, respectively). This reduction was greater in the mesenteric quadrant than in the other quadrants around the circumference. These results are indicative of a reduced capillary bed in the vicinity of anastomoses in intestine with chronic radiation damage, which might lead to an impeded blood supply and subsequent leakage.
Jahnson
S
S
Department of Urology, Orebro Medical Center, Sweden.
Christofferson
R H
RH
Gerdin
B
B
D000714:Anastomosis, Surgical; D000818:Animals; D002196:Capillaries / Q000528:radiation effects*; D007413:Intestinal Mucosa / Q000098:blood supply*; D007421:Intestine, Small / Q000098:blood supply / Q000528:radiation effects* / Q000601:surgery; D008297:Male; D011833:Radiation Injuries, Experimental / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley