Growth-dependent change of the 26S proteasome in budding yeast.
The 26S proteasome is assembled from the 20S proteasome and the regulatory subunit complex in an ATP-dependent manner. In the present study, we found that the ATP-dependent activity and the protein amount of the 26S proteasome change during growth of the budding yeast Saccharomyces cerevisiae. Both levels in the stationary phase are higher than those in the exponentially growing phase. On the other hand, the levels of the 20S proteasome appear to remain unchanged during growth. These results suggest that the 26S proteasome undergoes a growth-dependent change and that the 26S proteasome plays a role in the survival of yeast cells under starvation conditions.
Fujimuro
M
M
Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, 060-0812, Japan.
Takada
H
H
Saeki
Y
Y
Toh-e
A
A
Tanaka
K
K
Yokosawa
H
H
D000255:Adenosine Triphosphate / Q000494:pharmacology; D003546:Cysteine Endopeptidases / Q000032:analysis; D004789:Enzyme Activation; D005656:Fungal Proteins / Q000032:analysis; D015151:Immunoblotting; D009097:Multienzyme Complexes / Q000032:analysis; D010447:Peptide Hydrolases / Q000032:analysis*; D046988:Proteasome Endopeptidase Complex; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000254:growth & development*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D005656:Fungal Proteins; D009097:Multienzyme Complexes; D012967:Sodium Dodecyl Sulfate; D000255:Adenosine Triphosphate; D010447:Peptide Hydrolases; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease