[Effect of three kinds (tonifying kidney, invigorating spleen, promoting blood circulation) recipes on the hypothalamus-pituitary-adrenal-thymus (HPAT) axis and CRF gene expression].
OBJECTIVE
To observe the effect of tonifying Kidney (TK), invigorating Spleen (IS) and promoting blood circulation (PBC) recipes on the hypothalamus-pituitary-adrenal-thymus (HPAT) axis.


METHODS
Reverse-transcription polymerase chain reaction, radioimmunoassay and cellular immunity techniques were used to observe the effects of TH, IS, PBC on the neuroendocrine and immune system of this experimental model which HPAT axis was inhibited by corticosterone.


RESULTS
Only TK could avoid the depression of exogenous corticosterone, which by enhancing the expression of CRFmRNA in hypothalamus, and following the improvement of function of HPAT axis, that IS might have direct promotion on immune system, whereas without any effect of PBC on HPAT axis was observed.


CONCLUSIONS
TK, IS and PBC recipes have different effects on HPAT axis.
Zhong
L Y
LY
Huashan Hospital, Shanghai Medical University.
Shen
Z Y
ZY
Cai
D F
DF
D000818:Animals; D003345:Corticosterone; D003346:Corticotropin-Releasing Hormone / Q000096:biosynthesis* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression; D006854:Hydrocortisone / Q000097:blood; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects*; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism; D008297:Male; D008516:Medicine, Chinese Traditional; D010913:Pituitary-Adrenal System / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D016711:Yang Deficiency / Q000139:chemically induced* / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D003346:Corticotropin-Releasing Hormone; D003345:Corticosterone; D006854:Hydrocortisone