Immunization with the "immunogenic peptide" of TSH receptor induces oligoclonal antibodies with various biological activities.
Balb/c mice were immunized with a synthetic peptide (P354-14) corresponding to "the immunogenic peptide" of human thyrotropin receptor (hTSH-R). Through screening for binding to the peptide, we obtained several monoclonal antibodies with various biological activities: thyroid stimulation (SAb), inhibition of TSH stimulation (BAb) and no significant effect on cAMP production. One of the stimulatory clones was further studied. This clone enhanced cAMP production in Cos-7 cells transformed with the truncated TSH-R cDNA deleting the immunogenic peptide. These results indicated that the immunogenic peptide of the TSH-R induces oligoclonal anti-TSH-R antibodies, although the region is not essential for the functional epitope.
Sugawa
H
H
Department of Laboratory Medicine, Kyoto University School of Medicine, Sakyoku, Japan. hsugawa@kuhp.kyoto-u.ac.jp
Ueda
Y
Y
Ueda
M
M
Kosugi
S
S
Ichiyama
S
S
Mori
T
T
D000818:Animals; D001323:Autoantibodies / Q000096:biosynthesis* / Q000502:physiology*; D015551:Autoimmunity; D019556:COS Cells / Q000378:metabolism; D000242:Cyclic AMP / Q000502:physiology; D000939:Epitopes / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation / Q000235:genetics; D010455:Peptides / Q000276:immunology*; D011989:Receptors, Thyrotropin / Q000235:genetics / Q000276:immunology*; D013961:Thyroid Gland / Q000502:physiology; D013972:Thyrotropin / Q000502:physiology; D014162:Transfection
D001323:Autoantibodies; D000939:Epitopes; D010455:Peptides; D011989:Receptors, Thyrotropin; D013972:Thyrotropin; D000242:Cyclic AMP