Immunocompetence of human microvascular brain endothelial cells: cytokine regulation of IL-1beta, MCP-1, IL-10, sICAM-1 and sVCAM-1.
Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with gamma-interferon (IFN), tumour necrosis factor alpha (TNF-alpha) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1beta and soluble intercellular adhesion molecule-(sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-(sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-alpha and gamma-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after gamma-IFN stimulation.
Frigerio
S
S
Istituto Nazionale Neurologico C. Besta, Milan, Italy.
Gelati
M
M
Ciusani
E
E
Corsini
E
E
Dufour
A
A
Massa
G
G
Salmaggi
A
A
D000328:Adult; D000595:Amino Acid Sequence; D001327:Autoimmune Diseases / Q000276:immunology; D001921:Brain / Q000166:cytology / Q000276:immunology*; D018932:Chemokine CCL2 / Q000096:biosynthesis; D016207:Cytokines / Q000502:physiology*; D004727:Endothelium / Q000166:cytology / Q000276:immunology*; D005260:Female; D006801:Humans; D007121:Immunocompetence / Q000276:immunology*; D066298:In Vitro Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007371:Interferon-gamma / Q000494:pharmacology; D007375:Interleukin-1 / Q000096:biosynthesis; D008297:Male; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D018932:Chemokine CCL2; D016207:Cytokines; D007375:Interleukin-1; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma