IgG response to the immunoreactive region of parvovirus B19 nonstructural protein by immunoblot assay with a recombinant antigen.
A total of 190 serum samples from patients with different clinical manifestations in whom B19 parvovirus infection was suspected and 108 control serum samples were analyzed for the IgG response against parvovirus B19 nonstructural protein. An immunoblot assay was developed using the immunoreactive region of the B19 nonstructural protein as recombinant antigen. Virologic (B19 DNA) and serologic (IgM and IgG anti-VP) markers of B19 infection were also investigated. There was an even distribution of IgG nonstructural antibodies in serum samples of parvovirus B19-infected patients and controls (27.7% and 21.7%, respectively) that were mainly associated with patterns of past B19 infection.
Venturoli
S
S
Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Italy.
Gallinella
G
G
Manaresi
E
E
Gentilomi
G
G
Musiani
M
M
Zerbini
M
M
D000917:Antibody Formation; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D001168:Arthritis / Q000097:blood / Q000276:immunology / Q000821:virology; D005260:Female; D006402:Hematologic Diseases / Q000097:blood / Q000276:immunology / Q000821:virology; D006801:Humans; D015160:Hydrops Fetalis; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood*; D010322:Parvoviridae Infections / Q000097:blood / Q000276:immunology*; D016732:Parvovirus B19, Human / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000276:immunology / Q000821:virology; D011994:Recombinant Proteins / Q000276:immunology; D012016:Reference Values; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D017361:Viral Nonstructural Proteins