Measurements of zeta potentials of particulate biomaterials in protein-rich hyaluronan solution with changes in pH and protein constituents.
This study was undertaken to determine the zeta potentials of particulate biomaterials in three types of protein-rich hyaluronan solution with changes in pH; a microelectrophoretic method was used. For the purpose of determining the pH value of synovial fluid in various inflammatory conditions, we collected synovial fluid samples from joints with osteoarthritis (OA), rheumatoid arthritis (RA), and those undergoing revisions arthroplasties. The mean values of the pH in the synovial fluid from joints with OA, RA, and revision arthroplasty were shown to be 7.9, 7.5, and 8.1, respectively. The pH-zeta potential curves obtained differed, depending on the biomaterial and the medium. Addition of gamma-globulin to the medium reduced the absolute value of the zeta potentials of some of the biomaterials. The findings of this study suggest that the electrophoretic behaviors of the particulate biomaterials tested in this study are affected by the protein constituents of and pH changes in protein-rich synovial fluid. The values we obtained will be useful as reference standards and will also aid in the study of the surface phenomena of biomaterials.
Kitano
T
T
Department of Orthopaedic Surgery, Osaka City University, Medical School, Osaka, Japan. tkitano@med.osaka-cu.ac.jp
Ohashi
H
H
Kadoya
Y
Y
Kobayashi
A
A
Yutani
Y
Y
Yamano
Y
Y
D001672:Biocompatible Materials; D004563:Electrochemistry; D006820:Hyaluronic Acid / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry*; D012996:Solutions; D013582:Synovial Fluid / Q000737:chemistry
D001672:Biocompatible Materials; D011506:Proteins; D012996:Solutions; D006820:Hyaluronic Acid