Serum butyrylcholinesterase of non-human primate shows amine sensitive aryl acyl amidase and metallopeptidase activities and characteristics similar to those of the human serum enzyme.
Butyrylcholinesterase (BChE) was purified from monkey serum and the catalytic activities were examined. The enzyme has a molecular mass of approximately equal to 74 kDa as seen by SDS-gel electrophoresis. Monkey serum BChE also exhibits an amine sensitive aryl acylamidase (AAA) and a metallocarboxypeptidase activity. The tyramine activation of the aryl acylamidase activity and the metal chelator inhibition of the peptidase activity were characteristics similar to those of the human enzyme. Studies on 65Zn2+ binding and zinc chelate Sepharose chromatography showed that monkey serum BChE and human serum BChE have similar characteristics. Limited alpha chymotrypsin digestion of monkey serum BChE followed by Sephadex gel chromatography cleaved the enzyme into a 36 kDa fragment exhibiting peptidase activity. However the 20 kDa fragment corresponding to cholinesterase and aryl acylamidase activity was not detectable possibly due to the unstable nature of the fragment. Immunological studies showed that a polyclonal antibody against human serum BChE cross reacted with monkey serum BChE. The identical nature of the catalytic activities of human serum BChE and monkey serum BChE supports the postulate that all three catalytic activities co-exist in the same enzyme. This is the first time that purification and characterisation of the monkey serum BChE which has the highest sequence identity and immunological identity with that of human serum BChE, is being reported.
Bhanumathy
C D
CD
Neurochemistry Laboratory, Christian Medical College and Hospital, Vellore, India.
Rao
R V
RV
Balasubramanian
A S
AS
D000581:Amidohydrolases / Q000378:metabolism; D000588:Amines / Q000494:pharmacology*; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood*; D002268:Carboxypeptidases / Q000378:metabolism; D002918:Chymotrypsin / Q000378:metabolism; D004743:Enkephalin, Leucine / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D000882:Haplorhini; D008667:Metalloproteins / Q000378:metabolism; D010446:Peptide Fragments / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D000588:Amines; D004791:Enzyme Inhibitors; D008667:Metalloproteins; D010446:Peptide Fragments; D004743:Enkephalin, Leucine; D002091:Butyrylcholinesterase; D002268:Carboxypeptidases; D002918:Chymotrypsin; D000581:Amidohydrolases; D015032:Zinc