Simultaneous determination of diclofenac and oxybuprocaine in human aqueous humor with HPLC and electrochemical detection.
A sensitive and selective bioanalytical method for simultaneous determination of diclofenac and oxybuprocaine in human aqueous humor using reversed-phase HPLC and electrochemical detection is described. Chromatographic separation was achieved by using a Regis SPS 100 RP-8 column (5 microns; 150 x 4.6 mm I.D.). This support is coated with a hydrophilic polyoxyethylenepolymer. It allows protein-containing samples to be injected directly onto the column. The electrochemical detector permit a detection limit of 500 pg diclofenac per ml (daily relative standard deviation 6.3%) and 50 ng oxybuprocaine per ml (daily R.S.D. 2.6%), respectively. Results of administered and measured drug-concentrations in time dependent decrease are presented.
Kuhlmann
O
O
Martin-Luther-University, Halle-Wittenberg, Department of Biochemistry/Biotechnology, Germany.
Stoldt
G
G
Struck
H G
HG
Krauss
G J
GJ
D000779:Anesthetics, Local / Q000032:analysis*; D001082:Aqueous Humor / Q000737:chemistry*; D002387:Cataract Extraction; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016861:Cyclooxygenase Inhibitors / Q000032:analysis*; D004008:Diclofenac / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D006801:Humans; D011343:Procaine / Q000031:analogs & derivatives* / Q000032:analysis; D012680:Sensitivity and Specificity
D000779:Anesthetics, Local; D016861:Cyclooxygenase Inhibitors; D004008:Diclofenac; D011343:Procaine; C005298:benoxinate