Rapid identification of Mycobacterium tuberculosis and Mycobacterium avium by polymerase chain reaction and restriction enzyme analysis within sigma factor regions.
A single Polymerase Chain Reaction (PCR) within the rpoV gene was developed to rapidly distinguish mycobacteria isolated from clinical specimens. The species identifications of Mycobacterium avium complex (MAC) and Mycobacterium tuberculosis were congruent with standard typing techniques. The analysis was targeted toward the identification of species-specific markers for the clinically relevant M. tuberculosis and M. avium. In addition, HaeIII digestion of the amplification products yielded isolates-specific bands.
Comincini
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S
Dipartimento di Genetica e Microbiologia, Università di Pavia, Italy.
Barbarini
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D
Telecco
S
S
Bono
L
L
Marone
P
P
D017931:DNA Primers / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D015269:Mycobacterium avium Complex / Q000145:classification* / Q000235:genetics; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012808:Sigma Factor / Q000737:chemistry; D013045:Species Specificity; D014376:Tuberculosis / Q000175:diagnosis*
D017931:DNA Primers; D004269:DNA, Bacterial; D012808:Sigma Factor; D004262:DNA Restriction Enzymes