title: Primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum.
abstract: This is the first report on a primary culture of dispersed skin epidermal cells of rainbow trout Oncorhynchus mykiss Walbaum. These primary cells revealed a low seeding efficiency after 3 days (11.6 +/- 4.6%), whereas subcultured cells had a higher seeding efficiency at the same time point (75.5 +/- 34.0%) and increased in cell number (150-200% of seeded cells after 20 to 30 days). The cells were characterized applying histological, immunocytochemical and ultrastructural methods. The culture consisted of undifferentiated keratinocytes. Mucous cells as well as differentiated epithelial cells were absent. To date the cells were cultured for maximally 9 passages and 402 days and therefore provide the possibility for long-term studies.
authors.affiliation: Institute of Animal Pathology, University of Berne, Switzerland. lamche@itpa.unibe.ch
authors.forename: G
authors.initials: G
authors.lastname: Lamche
authors.forename: W
authors.initials: W
authors.lastname: Meier
authors.forename: M
authors.initials: M
authors.lastname: Suter
authors.forename: P
authors.initials: P
authors.lastname: Burkhardt-Holm
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D007633:Keratins / Q000378:metabolism; D008854:Microscopy, Electron; D017686:Oncorhynchus mykiss / Q000033:anatomy & histology* / Q000378:metabolism
chemical_list: D007633:Keratins