[Changes of calcium transport in rat liver nuclei during sepsis].
Calcium transport changes in rat liver nuclei were observed on the model of early sepsis (9 h after operation of cecal ligation and puncture). Calcium content in hepatocytes and nuclei were significantly increased by 20% and 36% respectively (P < 0.05) during sepsis. The activity of Ca(2+)-ATPase in hepatocytic nuclei was increased by 94% (P < 0.01) and 45Ca2+ transport accelerated by 32% (P < 0.01). There was a positive correlation between nuclear 45Ca2+ transport and nuclear Ca(2+)-ATPase activity (r = 0.914, P < 0.01). Calmodulin stimulated the activity of nuclear Ca(2+)-ATPase and 45Ca2+ transport; while calmodulin inhibitor trifluoperazine exerted an opposite effect. The above results suggest that liver nuclear calcium transport is strengthened during early sepsis as a result of changes of Ca(2+)-ATPase activity.
Wang
P Y
PY
Institute of Cardiovascular Basic Research, Beijing Medical University.
Ye
C
C
Pang
Y Z
YZ
Su
J Y
JY
Tang
C S
CS
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018805:Sepsis / Q000378:metabolism*
D000252:Calcium-Transporting ATPases; D002118:Calcium