Isolation and separation of HMG proteins and histones H1 and H5 and core histones by column chromatography on phosphocellulose.
A single-step method to dissociate histones as well as nonhistone chromosomal proteins from chicken erythrocyte chromatin and their separation into histones H1, H5, core histones, and high mobility group proteins by column chromatography on phosphocellulose is presented. NaCl at 2.0 M is effective in dissociating both histones and nonhistone proteins. The core histones elute as a complex. The pH is a critical factor in separating H5 from the core histones.
Garg
L C
LC
Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
Reeck
G R
GR
D000818:Animals; D002482:Cellulose / Q000031:analogs & derivatives* / Q000378:metabolism; D002645:Chickens; D002845:Chromatography / Q000379:methods; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry; D006609:High Mobility Group Proteins / Q000302:isolation & purification*; D006657:Histones / Q000302:isolation & purification*
D002868:Chromosomal Proteins, Non-Histone; D006609:High Mobility Group Proteins; D006657:Histones; D002482:Cellulose; C008881:phosphocellulose