Dietary administration of an extract from rosemary leaves enhances the liver microsomal metabolism of endogenous estrogens and decreases their uterotropic action in CD-1 mice.
We evaluated the effects of a methanol extract from the leaves of the plant Rosmarinus officinalis L. (rosemary) on the metabolism and action of estradiol and estrone. Treatment of female CD-1 mice with 2% rosemary in AIN-76A diet for 3 weeks increased the liver microsomal 2-hydroxylation of estradiol and estrone by approximately 150%, increased their 6-hydroxylation by approximately 30% and inhibited the 16alpha-hydroxylation of estradiol by approximately 50%. Treatment of female CD-1 mice with 2% rosemary diet for 3 weeks also stimulated the liver microsomal glucuronidation of estradiol and estrone by 54-67% and 37-56%, respectively. In additional studies, feeding 2% rosemary diet to ovariectomized CD-1 mice for 3 weeks inhibited the uterotropic action of estradiol and estrone by 35-50% compared with animals fed a control diet. The results of this study showed that feeding female mice a 2% rosemary diet increased the liver microsomal oxidation and glucuronidation of estradiol and estrone and inhibited their uterotropic action.
Zhu
B T
BT
Department of Chemical Biology, College of Pharmacy, Rutgers--The State University of New Jersey, Piscataway 08854-8020, USA.
Loder
D P
DP
Cai
M X
MX
Ho
C T
CT
Huang
M T
MT
Conney
A H
AH
D000818:Animals; D004032:Diet; D004967:Estrogens / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D006900:Hydroxylation; D019686:Lamiaceae / Q000737:chemistry*; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D039181:Steroid 16-alpha-Hydroxylase; D014599:Uterus / Q000502:physiology*
D004967:Estrogens; D005965:Glucuronates; D010936:Plant Extracts; D009249:NADP; D039181:Steroid 16-alpha-Hydroxylase