title: Stimulation of mucosal and systemic antibody responses against Bordetella pertussis filamentous haemagglutinin and recombinant pertussis toxin after nasal administration with chitosan in mice.
abstract: Mice were intranasally immunised with a mixture of Bordetella pertussis filamentous haemagglutinin (FHA) and recombinant pertussis toxin, PT-9K/129G (rPT) in combination with chitosan. For both antigens, this formulation induced systemic responses as measured by serum IgG and also mucosal responses as measured by secretory IgA in lung lavage and nasal washes. Immunosorbant assays were used to measure these responses. Both the systemic and mucosal responses were considerably higher than those produced when a mixture of rPT and FHA was administered nasally without chitosan. In comparison, intraperitoneally administered rPT/FHA adsorbed to Alhydrogel elicited only a systemic response, and nasal chitosan solution produced neither systemic nor mucosal response. This study clearly demonstrated that chitosan potentiated the serum and mucosal immune responses to nasally administered FHA and rPT in mice. Hence, this nasal chitosan delivery system has potential as a new non-injectable vaccine for the prophylaxis of whooping cough.
authors.affiliation: Danbiosyst UK Ltd, Albert Einstein Centre, Highfields Science Park, Nottingham, UK.
authors.forename: I
authors.initials: I
authors.lastname: Jabbal-Gill
authors.forename: A N
authors.initials: AN
authors.lastname: Fisher
authors.forename: R
authors.initials: R
authors.lastname: Rappuoli
authors.forename: S S
authors.initials: SS
authors.lastname: Davis
authors.forename: L
authors.initials: L
authors.lastname: Illum
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology*; D000276:Adjuvants, Immunologic; D000281:Administration, Intranasal; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology*; D002686:Chitin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000276:immunology; D048271:Chitosan; D005260:Female; D006388:Hemagglutinins / Q000276:immunology*; D018928:Immunity, Mucosal; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin; D010567:Pertussis Vaccine / Q000008:administration & dosage / Q000276:immunology*; D011994:Recombinant Proteins / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006388:Hemagglutinins; D010567:Pertussis Vaccine; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; D002686:Chitin; D048271:Chitosan; D037342:Pertussis Toxin