Analysis of the regulatory region of the lysC gene of Escherichia coli.
A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream the transcription start site of promoter P1. Both promoters are regulated by lysine.
Liao
H H
HH
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan.
Hseu
T H
TH
D001222:Aspartate Kinase / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D007763:Lac Operon / Q000235:genetics; D008239:Lysine / Q000096:biosynthesis; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
D011993:Recombinant Fusion Proteins; D001222:Aspartate Kinase; D001616:beta-Galactosidase; D008239:Lysine