title: Continuous perfusion of donor hearts in the beating state extends preservation time and improves recovery of function.
abstract: OBJECTIVE
  Improving methods of donor heart preservation may permit prolonged storage and remote procurement of cardiac allografts. We hypothesized that continuous, sanguineous perfusion of the donor heart in the beating, working state may prolong myocardial preservation.
  
  
  METHODS
  We developed a portable perfusion apparatus for use in donor heart preservation. Contractile, metabolic, and vasomotor functions were monitored simultaneously in an isolated swine heart. The metabolic state was monitored by myocardial tissue pH. Vasomotor function was assessed in isolated coronary ring chambers. Hearts were randomized into 3 groups: group I (n = 5), cardioplegic arrest, 12-hour storage at 4 degrees C with modified Belzer solution, and 2-hour sanguineous reperfusion in the working state; group II (n = 6), 12-hour continuous perfusion in the beating working state, 30 minutes of arrest (to simulate re-implantation time), and 2 hours of reperfusion, as above; group III (n = 7), coronary ring control hearts.
  
  
  RESULTS
  At 2 hours of reperfusion, left ventricular developed pressure in group II was higher than in group I (mean +/- standard deviation: 90 +/- 6 mm Hg, 53 +/- 15 mm Hg, P = .005). Significantly less myocardial edema was observed in group II than in group I (73% +/- 4%, 80% +/- 1% water content, P = .01). Significantly less myocardial acidosis was noted in group II than in group I during preservation (pH 7.3 +/- 0.01, 6.1 +/- 0.03, P < .001) and reperfusion (pH 7.3 +/- 0.008, 6.8 +/- 0.05, P < .001). Coronary endothelial vasomotor function was better preserved in group II than in group I as evidenced by dose-response relaxation of coronary rings to 10(-8) mol/L bradykinin (37%, 55% delta baseline, P = .01).
  
  
  CONCLUSIONS
  This new method extends the current preservation limit and avoids time-dependent ischemic injury, thereby allowing for distant procurement of donor organs.
authors.affiliation: Department of Surgery, Brockton/West Roxbury Veterans Administration Medical Center, Harvard Medical School, Boston, Mass 02132, USA.
authors.forename: W H
authors.initials: WH
authors.lastname: Hassanein
authors.forename: L
authors.initials: L
authors.lastname: Zellos
authors.forename: T A
authors.initials: TA
authors.lastname: Tyrrell
authors.forename: N A
authors.initials: NA
authors.lastname: Healey
authors.forename: M D
authors.initials: MD
authors.lastname: Crittenden
authors.forename: V
authors.initials: V
authors.lastname: Birjiniuk
authors.forename: S F
authors.initials: SF
authors.lastname: Khuri
mesh_terms: D000818:Animals; D003326:Coronary Circulation / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology; D006324:Heart Arrest, Induced; D016027:Heart Transplantation / Q000502:physiology*; D008297:Male; D009200:Myocardial Contraction / Q000502:physiology*; D015428:Myocardial Reperfusion Injury / Q000503:physiopathology; D009926:Organ Preservation / Q000295:instrumentation; D010477:Perfusion; D014022:Tissue Survival / Q000502:physiology