Cytokeratin expression in precancerous lesions of the human oral cavity.
Cytokeratin (CK) expression was studied in buccal mucosa (BM) from 20 leucoplakia and 7 submucous fibrosis patients using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and two-dimensional gel electrophoresis with iso-electric focussing (IEF) as the first dimension. Normal BM expresses CK 4, 5, 13, 14 and perhaps 19. Of 20 leucoplakia samples analysed, CK 5 was not detected in 17 samples, while CK 14 was not found in 13 samples. CK 1 and CK 8 were aberrantly expressed in six and seven samples, respectively. CK expression in contralaterally collected uninvolved tissues from 3 patients showed a normal pattern in two samples. Non-expression of CK 5 was observed in five of seven submucous fibrosis samples, while CK 14 was not detected in only two samples. CK 8 was aberrantly expressed in three samples. All the leucoplakia patients were chronic tobacco chewers. Thus, non-expression of CK 5 may be an early event occurring in tobacco-associated pathological changes in the BM.
Vaidya
M M
MM
Cell Biology Division, Cancer Research Institute, Tata Memorial Centre, Parel, Mumbai, India. cri3@soochak.ncst.ernet.in; bhise@soochak.ncst.ernet.in
Sawant
S S
SS
Borges
A M
AM
Ogale
S B
SB
Bhisey
A N
AN
D014408:Biomarkers, Tumor / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007633:Keratins / Q000378:metabolism*; D007972:Leukoplakia, Oral / Q000378:metabolism*; D009061:Mouth Mucosa / Q000378:metabolism; D009062:Mouth Neoplasms / Q000378:metabolism*; D009914:Oral Submucous Fibrosis / Q000378:metabolism*; D010947:Plants, Toxic; D011230:Precancerous Conditions / Q000378:metabolism*; D014030:Tobacco, Smokeless
D014408:Biomarkers, Tumor; D007633:Keratins