The renin-angiotensin system in hybrid NG108-15 cells. Renin gene is from mouse neuroblastoma, angiotensinogen and angiotensin-converting enzyme genes are of rat glioma origin.
Angiotensin II (Ang II) increases the level of tyrosine phosphorylation of several proteins in nondifferentiated NG108-15 cells, a hybrid derived from the fusion of mouse neuroblastoma and rat glioma cells. Conversely, incubation of NG108-15 cells with an angiotensin-converting enzyme (ACE) inhibitor decreased the basal level of tyrosine phosphorylation of proteins, suggesting that locally secreted Ang II may act as an autocrine regulator. By RT-PCR, we found that nondifferentiated NG108-15 cells contained the mRNA transcript of the rat angiotensinogen, mouse renin and rat ACE genes, thus confirming that NG108-15 cells contain all the elements of a local renin-angiotensin system.
Laflamme
L
L
Service of Endocrinology, Faculty of Medicine, University of Sherbrooke, Canada.
Brechler
V
V
Reudelhuber
T L
TL
Gallo-Payet
N
N
Deschepper
C F
CF
D000804:Angiotensin II / Q000494:pharmacology; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology; D000808:Angiotensinogen / Q000235:genetics*; D000818:Animals; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D005910:Glioma / Q000235:genetics*; D006822:Hybrid Cells / Q000378:metabolism; D017706:Lisinopril / Q000494:pharmacology; D051379:Mice; D009447:Neuroblastoma / Q000235:genetics*; D007703:Peptidyl-Dipeptidase A / Q000235:genetics*; D010766:Phosphorylation / Q000187:drug effects; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D012084:Renin-Angiotensin System / Q000235:genetics*
D000806:Angiotensin-Converting Enzyme Inhibitors; D017931:DNA Primers; D012333:RNA, Messenger; D000808:Angiotensinogen; D000804:Angiotensin II; D017706:Lisinopril; D007703:Peptidyl-Dipeptidase A