High-level expression of eukaryotic polypeptides from bacterial chromosomes.
Attractive economics and short development timelines have often been cited as reasons for using bacteria to express eukaryotic proteins on a commercial scale. Nevertheless, routine techniques for bacterial expression of heterologous proteins are beset by a variety of technical and legal difficulties. In particular, the use of plasmids to express foreign proteins, popular promoter systems, protein fusion partners, and histidine tags and the recovery of proteins from inclusion bodies are affected by a host of issued patents. Chromosomally encoded leaderless fusions (CELF) offer a variety of technical and legal advantages over existing bacterial expression systems. In this study, we show that CELF can be used to produce a wide assortment of eukaryotic proteins at 10-liter fermentation scale.
Olson
P
P
Department of Molecular and Cell Biology, Celtrix Pharmaceuticals, 3055 Patrick Henry Drive, Santa Clara, California, 95054, USA.
Zhang
Y
Y
Olsen
D
D
Owens
A
A
Cohen
P
P
Nguyen
K
K
Ye
J J
JJ
Bass
S
S
Mascarenhas
D
D
D017123:Bacteriophage T7 / Q000235:genetics; D019149:Bioreactors; D002875:Chromosomes / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods; D004926:Escherichia coli / Q000378:metabolism*; D005057:Eukaryotic Cells / Q000737:chemistry; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D010330:Patents as Topic / Q000331:legislation & jurisprudence; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000191:economics / Q000302:isolation & purification*
D011993:Recombinant Fusion Proteins