title: IL-2 expansion of T and NK cells from growth factor-mobilized peripheral blood stem cell products: monocyte inhibition.
abstract: The expansion of T and natural killer (NK) cells in growth factor-mobilized peripheral blood stem cell (PSC) products with interleukin-2 (IL-2) requires a reduction in monocyte frequency. Monocytes are enriched with stem cells during apheresis and, in this series of growth factor-mobilized PSC products from breast cancer patients, represented 36 +/- 6% of the cells in the product. Immunophenotyping studies revealed that monocytes inhibited the proliferation of NK cells (CD56+ and CD3- CD8+ CD56+ cells) and T cells (CD3+, CD4+, and CD8+ cells) during IL-2 co-culture for 7, 14, or 21 days. A reduction in monocytes resulted in 61-fold expansion of CD3- CD8+ CD56+ cells compared with a 3.7-fold increase of CD3+ cells by day 21. In addition, following IL-2 co-culture, cells from PSC products with a reduced frequency of monocytes had a significantly increased T cell mitogenic response and NK cell activity in PSC products compared with intact products. We suggest that monocytes inhibit the IL-2-dependent proliferation and augmented function of NK and T cells from growth factor-mobilized PSC products.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.
authors.forename: A G
authors.initials: AG
authors.lastname: Ageitos
authors.forename: R K
authors.initials: RK
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Ino
authors.forename: I
authors.initials: I
authors.lastname: Ozerol
authors.forename: S
authors.initials: S
authors.lastname: Tarantolo
authors.forename: E K
authors.initials: EK
authors.lastname: Reed
authors.forename: J E
authors.initials: JE
authors.lastname: Talmadge
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects*; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D005260:Female; D019650:Hematopoietic Stem Cell Mobilization; D006801:Humans; D016130:Immunophenotyping; D007376:Interleukin-2 / Q000494:pharmacology*; D007694:Killer Cells, Natural / Q000187:drug effects*; D009000:Monocytes / Q000187:drug effects*
chemical_list: D007376:Interleukin-2