Fatty acid alpha-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (Cucumis sativus).
Fatty acid alpha-oxidation in cucumber (Cucumis sativus) involves enzymatic conversion of long-chain Cn-fatty acids to the C(n-1)-aldehyde and CO2. However, the mechanism of this process is not well understood. In this study, the alpha-oxidation of the fatty acid analogues tetradecylthioacetic acid (TTA) and tetradecylthiopropionic acid (TTP) with a sulphur atom substituting the methylene group in positions 3 and 4, respectively, was investigated and compared to palmitic acid. Both [1-14C]TTA and [1-14C]TTP could be alpha-oxidised in the cucumber subcellular 150000xgmax fraction. [1-14C]TTP was an even better substrate compared to the natural palmitic acid, while [1-14C]TTA was alpha-oxidised to a lower extent. [2-14C]TTA revealed no 14CO2, indicating that only one cycle of alpha-oxidation occurred. TTA was an inhibitor of the palmitic acid alpha-oxidation, and the inhibitory effects were examined.
Andersen Borge
G I
GI
Norwegian Food Research Institute, Osloveien 1, N-1430 As, Norway. grethe.andersen.borge@inf.nlh.no
Slinde
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E
Nilsson
A
A
D000818:Animals; D002250:Carbon Radioisotopes; D002851:Chromatography, High Pressure Liquid; D018553:Cucumis sativus / Q000378:metabolism* / Q000648:ultrastructure; D005227:Fatty Acids / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006900:Hydroxylation; D007850:Lauric Acids / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D019308:Palmitic Acid / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013440:Sulfides / Q000378:metabolism*
D002250:Carbon Radioisotopes; D005227:Fatty Acids; D007850:Lauric Acids; D011422:Propionates; D013440:Sulfides; C030358:lauric acid; C062077:1-(carboxyethylthio)tetradecane; D019308:Palmitic Acid; D009249:NADP; C062078:1-(carboxymethylthio)tetradecane