[Comparison of sequences of hepatitis B virus S gene fragment in eight cities of China].
Sequencing and comparative analysis of 52nd to 512th nucleotide of S gene in hepatitis B virus (HBV) were conducted in 43 cases of sero-positive and 52 cases of sero-negative HB from eight cities, including Changchun, Datong, Hangzhou, Shenzhen, Qingtao, Xi'an, Kunming and Lahsa, to study the relationship between focal variation of HBV S gene and seronegative HB. It was found that sequences of HBV S gene fragment in the same genotype were more conservative, most of its mutation happened randomly, and no insertion, deletion or null mutation occurred. There were three and five hypervariable nucleotide points in HBV isolated from two different HB groups with identical genotype and serotype (C genotype/adr serotype and C genotype/ayw1 serotype), respectively, and there were five hypervariable nucleotide points in HBV of D genotype/ayw1 serotype (five strains of sero-positive HBV) and D genotype/ayw3 serotype (ten strains of sero-negative HBV), respectively. It suggests that most cases of sero-negative HB may correlate with low level of HBV in their blood, and a small number of cases may be caused by HBV S gene mutation.
Fan
J
J
Department of Microbiology, Beijing Medical University.
Zhuang
H
H
Li
Y
Y
D002681:China; D005814:Genes, Viral / Q000235:genetics*; D005838:Genotype; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D017354:Point Mutation; D017422:Sequence Analysis, DNA
D006514:Hepatitis B Surface Antigens