title: Cys25-nitrosylation inactivates papain.
abstract: Nitric oxide (NO) may modulate the catalytic activity of cysteine proteases. In the present study, the inhibitory effect of NO, released by the NO-donors (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide and nitroprusside, on papain action is reported. Papain inactivation via NO-mediated nitrosylation of the Cys25 catalytic residue represents a molecular model for cysteine protease inhibition.
authors.affiliation: Department of Biology, University of Rome Tre, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Venturini
authors.forename: E
authors.initials: E
authors.lastname: Fioravanti
authors.forename: M
authors.initials: M
authors.lastname: Colasanti
authors.forename: T
authors.initials: T
authors.lastname: Persichini
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
mesh_terms: D003545:Cysteine / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology*; D009574:Nitro Compounds / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010206:Papain / Q000037:antagonists & inhibitors* / Q000378:metabolism*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D009574:Nitro Compounds; D009599:Nitroprusside; D009569:Nitric Oxide; C061872:FK 409; D010206:Papain; D003545:Cysteine