title: Structural changes of IgG induced by heat treatment and by adsorption onto a hydrophobic Teflon surface studied by circular dichroism spectroscopy.
abstract: Thermal denaturation of mouse monoclonal immunoglobulin G (isotype 1), as well as structural rearrangements resulting from adsorption on a hydrophobic Teflon surface, are studied by circular dichroism spectroscopy. Both heat-induced and adsorption-induced denaturation do not lead to complete unfolding into an extended polypeptide chain, but leave a significant part of the IgG molecule in a globular or corpuscular form. Heating dissolved IgG causes a decrease of the fractions of beta-sheet and beta-turn conformations, whereas those of random coil and, to a lesser extent, alpha-helix increase. Adsorption enhances the formation of alpha-helices and random coils, but the beta-sheet content is strongly reduced. Heating adsorbed IgG results in a gradual break-down of the alpha-helix and beta-turn contents, and a concomitant formation of beta-sheet structures. Thus, the structural changes in IgG caused by heating and by adsorption, respectively, are very different. However, after heating, the structure of adsorbed IgG approaches the structure of thermally denatured IgG in solution.
authors.affiliation: Laboratory for Physical Chemistry and Colloid Science, Wageningen Agricultural University, The Netherlands. ronald.vermeer.rv@bayer_ag.de
authors.forename: A W
authors.initials: AW
authors.lastname: Vermeer
authors.forename: M G
authors.initials: MG
authors.lastname: Bremer
authors.forename: W
authors.initials: W
authors.lastname: Norde
mesh_terms: D000327:Adsorption; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002942:Circular Dichroism; D004355:Drug Stability; D006358:Hot Temperature; D007074:Immunoglobulin G / Q000737:chemistry*; D051379:Mice; D011138:Polytetrafluoroethylene; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D013499:Surface Properties; D013816:Thermodynamics
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D011138:Polytetrafluoroethylene