Pulp and root development after partial extrusion in immature rat molars: a histopathological study.
Experimental studies were undertaken in immature rat molar teeth to investigate the relationship between root development and the prognosis after partial extrusion. The experimental partial extrusion was induced at 1/2 root formation, 2/3 root formation and 3/4 root formation, using an extrusion device designed by the author. Histopathological studies and SEM observation of the pulpal tissue in rat maxillary first molars with partially formed roots after partial extrusion were conducted and evaluated. The degree of extrusion was changed according to the development of the root to produce a similar luxated state. The rats were sacrificed immediately after experimental extrusion, immediately after operation, and after 3 days, 7 days, 14 days, 30 days, and 60 days to compare the histopathological and SEM findings between experimental groups and control groups. Immediately after the extrusive injury, ablations of the odontoblast layer from dentin were seen with retention of a fluid component in the ablated spaces in the pulp. On 1 and 3 days after surgery, cellular components such as erythrocytes and leukocytes due to bleeding were seen. Ablations of the odontoblast layer were seen in the coronal pulp most frequently in the pulp horn, cervical area and floor of the pulp chamber. In general, pulp reaction after extrusion in groups with more complete root formation was more severe than in groups with less complete root formation. It is suggested that the more complete the root formation, the greater the effect on the pulp as result of partial extrusion, while with less complete root formation, the greater the malformation of the root apices.
Shibue
T
T
Department of Pediatric Dentistry, Fukuoka Dental College, Japan. sibutaku@college.fdcnet.ac.jp
Taniguchi
K
K
Motokawa
W
W
D000818:Animals; D003739:Dental Cementum / Q000473:pathology; D003782:Dental Pulp / Q000098:blood supply / Q000473:pathology*; D003786:Dental Pulp Cavity / Q000473:pathology; D003790:Dental Pulp Necrosis / Q000209:etiology; D003804:Dentin / Q000473:pathology; D003809:Dentin, Secondary / Q000503:physiopathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013997:Time Factors; D019227:Tooth Apex / Q000254:growth & development / Q000503:physiopathology; D014084:Tooth Avulsion / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D014092:Tooth Root / Q000254:growth & development / Q000293:injuries*