title: Hierarchical order in a satellite DNA from the European brown frog Rana dalmatina.
abstract: We have cloned and sequenced a 341 bp EcoRV fragment of a repetitive DNA from the European brown frog Rana dalmatina. This fragment contained 11 copies in tandem of the 31 bp long repeat of a satellite DNA, that was named RdS2. RdS2 is species-specific, is not A+T rich and represents about 0.24% of the frog genome (3.5 x 10(5) copies of repeats per haploid genome). Southern blots of Rana dalmatina. DNA partially digested with EcoRV and RsaI indicated that RdS2 also contains high-order repeats with periodicities varying from 10 to 26 copies of the 31 bp unit repeat. Thus, RdS2 appears to have a hierarchical order like a number of satellite DNAs from other vertebrate and invertebrate genomes.
authors.affiliation: Dipartimento di Medicina Clinica e Sperimentale, Facoltà di Medicine e Chirurgia, Università di Napoli Federico II.
authors.forename: D E
authors.initials: DE
authors.lastname: Cardone
authors.forename: I
authors.initials: I
authors.lastname: Feliciello
authors.forename: G
authors.initials: G
authors.lastname: Chinali
mesh_terms: D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004276:DNA, Satellite