Identification of rat OX40 ligand by molecular cloning.
OX40 (CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily first identified as a rat T cell activation marker. In the present study, we identified the rat ligand for OX40 (OX40L) by molecular cloning. Rat OX40L cDNA was cloned from a HTLV-1-transformed rat T cell line by cross-hybridization with mouse OX40L cDNA. The predicted rat OX40L polypeptide is composed of 199 amino acids, showing 80.9 and 43.3% homology to mouse and human OX40L, respectively. Expression of rat OX40L mRNA was found in HTLV-1-transformed rat T cell lines. Expression of OX40L on the cell surface of these HTLV-1-transformed rat T cell lines was also demonstrated by flow cytometric analysis with a soluble fusion protein composed of the extracellular region of the Fc portion of human IgG (OX40-Ig). To explore the function of rat OX40L, we generated cDNA transfectants stably expressing rat OX40L. The rat OX40L transfectants exhibited a potent costimulatory activity for proliferation and IL-2 production of anti-CD3-stimulated rat T cells. These results indicated that rat OX40L can provide an efficient costimulation for rat T cells and that it may be involved in HTLV-1-associated pathologies in the rat system as has been suggested in the human system.
Akiba
H
H
Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Atsuta
M
M
Yagita
H
H
Okumura
K
K
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002455:Cell Division; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D003001:Cloning, Molecular; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D015368:Human T-lymphotropic virus 1; D006801:Humans; D007668:Kidney; D008024:Ligands; D008562:Membrane Glycoproteins; D051379:Mice; D008969:Molecular Sequence Data; D053278:OX40 Ligand; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D014407:Tumor Cells, Cultured; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism*; D048069:Tumor Necrosis Factors
D016207:Cytokines; D008024:Ligands; D008562:Membrane Glycoproteins; D053278:OX40 Ligand; D012333:RNA, Messenger; D053262:Receptors, OX40; D018124:Receptors, Tumor Necrosis Factor; C505716:TNFRSF4 protein, human; C089251:TNFSF4 protein, human; C505717:Tnfrsf4 protein, mouse; C505718:Tnfrsf4 protein, rat; C504586:Tnfsf4 protein, mouse; C504587:Tnfsf4 protein, rat; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D048069:Tumor Necrosis Factors