Suppression of the 5' splice site mutation in the Nagase analbuminemic rat with mutated U1snRNA.
Nagase analbuminemic rats (NAR) are deficient in serum albumin due to skipping of the albumin exon H caused by a mutation in the intron HI. This mutation deletes nucleotides from +5 to +11 in the 5' splice site region, where it interacts with U1snRNA. To determine whether the mutation can be suppressed by the compensatory base substitution in U1snRNA, we constructed mutated U1snRNA genes with various degrees of complementarity to the mutated 5' splice site. Several mutated U1snRNA genes activated the mutated 5' splice site of the intron HI, when cotransfected with the albumin minigene derived from NAR. In vivo activity of these mutated U1snRNAs correlated well with the predicted thermodynamic stability. Since mutation in the 5' splice site is one of common causes of genetic defects in human (5), these data indicate that NAR is a good model system to examine the possibility of gene therapy using a mutated U1snRNA.
Hitomi
Y
Y
Investigative Treatment Division, National Cancer Center Research Institute East, 6-5-1, Kashiwanoha, Chiba, Kashiwa, 277, Japan.
Sugiyama
K
K
Esumi
H
H
D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D017353:Gene Deletion; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D012326:RNA Splicing / Q000235:genetics*; D012342:RNA, Small Nuclear / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D011922:Rats, Mutant Strains; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000172:deficiency* / Q000235:genetics*; D013489:Suppression, Genetic / Q000502:physiology*; D014162:Transfection
D012342:RNA, Small Nuclear; D012709:Serum Albumin