[Role of endogenous endothelins in renal injury in burnt rats].
Endothelins are potent vasoconstrictors released by endothelium, and their concentrations, which may be involved in the pathophysiology of burn injury, are increased in burn patients. The purpose of this study was to investigate the role of endogenous endothleins in postburn renal injury. 30% TBSA III degree burnt rat model was used, and 72 male Wistar rats were studied: 32 burnt rats received intravenous infusion of the non-selective ETA-ETB receptor antagonist PD145065 (1 mumol/Kg body weight) for 3 hours (group 1); other 32 burnt rats received only the vehicle (0.5 ml/250 g/h) (group 2). The rats both in group 1 and group 2 were given adequate resuscitation via different ways. 8 healthy rats were normal controls. In group 2, the blood flow of kidney decreased sharply, while marked lipoperoxidation damage and ATP depletion in kidney occurred although the arterial blood pressure remained unchanged postburn after sufficient fluid was given. Administration of endothelin receptor antagonist PD145065 has been demonstrated to be able to prevent kidney from damage to some extents both morphologically and biochemically by means of increment of blood flow, stimulation of the activity of SOD, enhancement of the contents of ATP and ADP, and reduction of the severity of edema in kidney. These results suggest that endogenous endothelins contribute to renal injury in the early postburn stage in rats through vascular constriction and tissue hypoxia which in turn induced ATP depletion and lipoperoxidation damage.
Gao
J
J
Burn Center of People's Liberation Army, 304 Hospital, Beijing.
Wu
X
X
Yang
Z
Z
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000378:metabolism*; D007668:Kidney / Q000473:pathology*; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D009842:Oligopeptides; C082887:PD 145065; D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase