title: Intramuscular 3-hydroxybutyrate levels after 60 tetani/min contraction in diabetic and non-diabetic rats.
abstract: Post-exercise hyperketonemia in poorly-controlled diabetic patients is a well recognized phenomenon, but because studies concerning changes in ketone body metabolism in muscle during and/or after exercise are scarce, we measured the intramuscular 3-hydroxybutyrate (3-OHBA) and lactate concentrations in 12 diabetic (streptozotocin-induced; 60 mg/kg, ip) and 13 non-diabetic control rats before and after a 1-h muscle contraction. One thigh tetanic contraction was elicited at a tetanic frequency of 60 tetani/min and the other thigh was kept resting. We used a microdialysis technique in both quadriceps muscles, vastus lateralis, and the right jugular vein. Blood flow in both femoral muscles was also monitored before and after contraction. Dialysate 3-OHBA and lactate levels in contracting and non-contracting muscles and in blood showed a significant increase after contraction (P<0.05) in diabetic rats. In control rats there was no significant change in dialysate 3-OHBA or the lactate concentration in contracting and non-contracting muscles or in blood before and after contraction. A significant increase in contracting muscle blood flow was observed only for the first 5 min after contraction in diabetic and control rats. These results suggest that 3-OHBA uptake in contracting muscle is reduced, and that this phenomenon may play a role in post-exercise ketosis in diabetes.
authors.affiliation: Research Center of Health, Physical Fitness and Sports, Nagoya University, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Oshida
authors.forename: J
authors.initials: J
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Nakai
authors.forename: Y
authors.initials: Y
authors.lastname: Shimomura
authors.forename: I
authors.initials: I
authors.lastname: Ohsawa
authors.forename: Y
authors.initials: Y
authors.lastname: Sato
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000378:metabolism*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values
chemical_list: D019344:Lactic Acid; D020155:3-Hydroxybutyric Acid