Differences in the isodesmin pattern between the electric organs of Electrophorus electricus L.
Desmin, the intermediate filament protein of muscle, is present in the electric organs of Electrophorus electricus L. as five isovariants, instead of the one to two isovariants found in muscle. We analyzed the isodesmin pattern in the three different electric organs using densitometry of Coomassie blue-stained bands in electrofocusing polyacrylamide gel electrophoresis. We were able to compare the relative amount of each of the five desmin isovariants in an isodesmin pattern characteristic of each electric organ. These patterns proved to be, in some cases, statistically different. Desmin in each electric organ could have slightly different functions in order to correlate with the organ-specific isovariant patterns.
Costa
M L
ML
Departamento de Histologia e Embriologia, Universidade Federal do Rio de Janeiro, Brazil. mlcosta@chagas. biof.ufrj.br
Mermelstein
C S
CS
Fróes
M M
MM
Chagas
C
C
Moura Neto
V
V
D000818:Animals; D003720:Densitometry; D003893:Desmin / Q000737:chemistry*; D004557:Electric Organ / Q000737:chemistry*; D004593:Electrophorus / Q000378:metabolism*; D007382:Intermediate Filaments / Q000737:chemistry; D007525:Isoelectric Focusing
D003893:Desmin