Involvement of the Saccharomyces cerevisiae UTH1 gene in the oxidative-stress response.
The UTH1 gene was identified by screening a Saccharomyces cerevisiae promoter-probe gene bank for oxidative stress-responsive genes. Transcription of UTH1 was decreased by the superoxide anion and increased by hydrogen peroxide. Deletion of UTH1 did not affect the growth of grande cells, however in a rho0 background it caused retarded growth. The uth1 mutant showed increased resistance to peroxides and, in contrast, was sensitive to superoxide or the thiol oxidant diamide. Furthermore, the mutant exhibited increased survival under starvation conditions, with elevated levels of dormant cells in starved cell cultures. A multicopy plasmid containing the first half of the ORF could confer increased resistance to superoxide and increased sensitivity to peroxides/diamide/starvation on wild-type cells. The same plasmid in the uth1 background caused a highly increased mortality.
Bandara
P D
PD
Cooperative Research Centre for Food Industry Innovation and School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, NSW 2052, Australia.
Flattery-O'Brien
J A
JA
Grant
C M
CM
Dawes
I W
IW
D000595:Amino Acid Sequence; D002470:Cell Survival / Q000235:genetics; D003001:Cloning, Molecular; D003958:Diamide / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005800:Genes, Fungal / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D018384:Oxidative Stress / Q000235:genetics*; D010269:Paraquat / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013481:Superoxides / Q000494:pharmacology
D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; C116477:UTH1 protein, S cerevisiae; D003958:Diamide; D013481:Superoxides; D006861:Hydrogen Peroxide; D010269:Paraquat