Regulation of Zn-alpha2-glycoprotein-mediated cell adhesion by kininogens and their derivatives.
MC3T3-E1 (mouse osteoblast-like) cells adhered to a tissue culture plate coated with human Zn-alpha2-glycoprotein (Znalpha2gp). The adhesion of MC3T3-E1 cells to Znalpha2gp was inhibited by synthetic peptides such as RGDS and ELRGDV, and by antibody against vitronectin receptor. These findings suggested that the RGD region of Znalpha2gp interacts with the vitronectin receptor (alphavbeta3) on the MC3T3-E1 cell surface. Furthermore, we found that the common heavy chain of both HMW- and LMW-kininogens accelerated the Znalpha2gp-mediated MC3T3-E1 cell adhesion. Among the three domains of the common heavy chain of both kininogens, domain 3 promoted the cell adhesion by up to 200%. Among the nine synthetic peptides covering domain 3, the peptide, N334AEVYVVPWEKKIYPTVN351 accelerated in a dose-dependent manner the Znalpha2gp- and vitronectin (VN)-mediated MC3T3-E1 cell adhesion. These findings suggested that a defined region of domain 3 is responsible for the acceleration of cell adhesion.
Ogikubo
O
O
Department of Orthopedics, Nagoya City University Medical School, Nagoya, Mizuho-ku, 467-8601, Japan.
Maeda
T
T
Yamane
T
T
Ohtsuka
T
T
Ohkubo
I
I
Takahashi
S
S
Ohnishi
S
S
Matsui
N
N
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D007704:Kininogens / Q000737:chemistry / Q000494:pharmacology / Q000502:physiology*; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D019096:Vitronectin / Q000502:physiology; D060749:alpha-2-HS-Glycoprotein
C556354:AHSG protein, human; C556357:Ahsg protein, mouse; D001798:Blood Proteins; D007704:Kininogens; D046911:Macromolecular Substances; D010446:Peptide Fragments; D019096:Vitronectin; D060749:alpha-2-HS-Glycoprotein