Bacterial extract OM85-BV induces interleukin-12-dependent IFN-gamma production by human CD4+ T cells.
To obtain insight into the possible mode of action of bacterial extracts used as immunostimulants in Europe, we used the ELISPOT technique to investigate the effects of one of them (OM85-BV, Broncho-Vaxom) on interferon-y (IFN-gamma) production by peripheral blood mononuclear cells (PBMC). We found that (1) OM85-BV stimulates IFN-gamma secretion by PBMC from normal individuals and human immunodeficiency virus (HIV)-infected patients, (2) CD4+ cells represent the major source of IFN-gamma produced in response to OM85-BV, and (3) this effect of OM85-BV involves the induction of interleukin-12 (IL-12) secretion by accessory cells. We conclude that bacterial extracts might enhance antimicrobial defenses by eliciting IL-12-dependent IFN-gamma synthesis by CD4+ T cells.
Byl
B
B
Department of Immunology, Hôpital Erasme, Brussels, Belgium.
Libin
M
M
Gérard
M
M
Clumeck
N
N
Goldman
M
M
Mascart-Lemone
F
F
D000163:Acquired Immunodeficiency Syndrome / Q000503:physiopathology; D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D001419:Bacteria; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D016022:Case-Control Studies; D002457:Cell Extracts; D006801:Humans; D016130:Immunophenotyping; D007369:Interferon Inducers / Q000494:pharmacology*; D007371:Interferon-gamma / Q000096:biosynthesis*; D018664:Interleukin-12 / Q000378:metabolism / Q000502:physiology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D012016:Reference Values; D000095983:Bacterial Lysates
D000276:Adjuvants, Immunologic; D002457:Cell Extracts; D007369:Interferon Inducers; D007371:Interferon-gamma; D018664:Interleukin-12; C030259:Broncho-Vaxom; D000095983:Bacterial Lysates