Actions of tachykinins on the ion transport across the frog skin.
The tachykinin-dependent stimulation of ion transport across frog skin was studied. Tachykinin stimulation was due to interaction with an NK1-like receptor as [Sar9-Met(O2)11]-Substance P (a very selective NK1 agonist) strongly stimulated SCC, whereas [beta-Ala8]-Neurokinin A 4-10 (a very selective NK2 agonist) did not. The rank order of tachykinin potency was: PG-KI > Uperolein > Hylambatin > Kassinin > Phyllomedusin > [Sar9-Met(O2)11]-Substance P > Ranatachykinin A > Physalaemin > Ranakinin > Substance P and Eledoisin >> Neurokinin A. Neurokinin B, Scyliorhinin I, Urechistachykinin I and Urechistachykinin II had no effect. We conclude that the minimal structural requirements for stimulating SCC in the frog skin were the presence of: a) the C-terminal sequence Phe-X-Gly-Leu-Met-NH2; b) at least one Pro residue in the N-terminal sequence.
Lippe
C
C
Dipartimento di Fisiologia Generale ed Ambientale, Università di Bari, Italy. c.lippe@biologia.uniba.it
Lobasso
S
S
Cassano
G
G
Bellantuono
V
V
Ardizzone
C
C
D000595:Amino Acid Sequence; D000818:Animals; D017136:Ion Transport / Q000502:physiology*; D011893:Rana esculenta / Q000502:physiology*; D012879:Skin Physiological Phenomena; D015320:Tachykinins / Q000737:chemistry / Q000502:physiology*
D015320:Tachykinins