title: Molecular composition of the 16S toxin produced by a Clostridium botulinum type D strain, 1873.
abstract: The 16S toxin was purified from a Clostridium botulinum type D strain 1873 (D-1873). Furthermore, the entire nucleotide sequences of the genes coding for the 16S toxin were determined. It became clear that the purified D-1873 16S toxin consists of neurotoxin, nontoxic nonhemagglutinin (NTNH), and hemagglutinin (HA), and that HA consists of four subcomponents, HA1, HA2, HA3a, and HA3b, the same as type D strain CB16 (D-CB16) 16S toxin. The nucleotide sequences of the nontoxic components of these two strains were also found to be identical except for several bases. However, the culture supernatant and the purified 16S toxin of D-1873 showed little HA activity, unlike D-CB16, though the fractions successively eluted after the D-1873 16S toxin peak from an SP-Toyopearl 650S column showed a low level of HA activity. The main difference between D-1873 and D-CB16 HA molecules was the mobility of the HA1 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Therefore it was presumed that the loss of HA activity of D-1873 16S toxin might be caused by the differences of processing HA after the translation.
authors.affiliation: Department of Bacteriology, Okayama University Medical School, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Ikeda
authors.forename: Y
authors.initials: Y
authors.lastname: Fujinaga
authors.forename: H
authors.initials: H
authors.lastname: Sunagawa
authors.forename: K
authors.initials: K
authors.lastname: Takeshi
authors.forename: T
authors.initials: T
authors.lastname: Ohyama
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: K
authors.initials: K
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Oguma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001427:Bacterial Toxins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D001905:Botulinum Toxins / Q000235:genetics* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D003014:Clostridium botulinum / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005798:Genes, Bacterial / Q000235:genetics; D006386:Hemagglutination Tests; D006388:Hemagglutinins / Q000032:analysis / Q000235:genetics; D015151:Immunoblotting; D051379:Mice; D008969:Molecular Sequence Data; D009498:Neurotoxins / Q000032:analysis / Q000235:genetics; D016133:Polymerase Chain Reaction
chemical_list: C116382:16S toxin, Clostridium botulinum; D001427:Bacterial Toxins; D006388:Hemagglutinins; D009498:Neurotoxins; D001905:Botulinum Toxins