title: Circadian changes of type II adenylyl cyclase mRNA in the rat suprachiasmatic nuclei.
abstract: Circadian functions of the suprachiasmatic nuclei (SCN) are influenced by cyclic AMP (cAMP). Adenylyl cyclase type II (AC-II) is a cAMP-generating enzyme which, in the context of activation by Gsalpha, is further stimulated by protein kinase C or G protein betagamma subunits. Using in situ hybridization we have found a biphasic variation in AC-II mRNA within the rat SCN during the light-dark cycle (peaks at Zeitgeber time 6 and 18) and also in constant darkness (peaks at circadian time 2 and 14). The cingulate cortex showed no such variation. These findings suggest that circadian changes in AC-II expression may be pertinent to the rhythmic functions of the SCN.
authors.affiliation: Anatomy and Human Biology, Division of Biomedical Sciences, King's College London, London, WC2R 2LS, UK.
authors.forename: F R
authors.initials: FR
authors.lastname: Cagampang
authors.forename: F A
authors.initials: FA
authors.lastname: Antoni
authors.forename: S M
authors.initials: SM
authors.lastname: Smith
authors.forename: H D
authors.initials: HD
authors.lastname: Piggins
authors.forename: C W
authors.initials: CW
authors.lastname: Coen
mesh_terms: D000262:Adenylyl Cyclases / Q000096:biosynthesis*; D000818:Animals; D001345:Autoradiography; D002940:Circadian Rhythm / Q000502:physiology*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D017208:Rats, Wistar; D013493:Suprachiasmatic Nucleus / Q000378:metabolism*
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D000262:Adenylyl Cyclases