Water-soluble conjugate of double-stranded DNA and Poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins.
Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 degreesC. This system was used for the one-pot separation of restriction endonuclease EcoRI.
Umeno
D
D
Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Kawasaki
M
M
Maeda
M
M
D000180:Acrylic Resins / Q000737:chemistry*; D002846:Chromatography, Affinity; D004247:DNA / Q000737:chemistry*; D004268:DNA-Binding Proteins / Q000302:isolation & purification*; D015246:Deoxyribonuclease EcoRI / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D011564:Furocoumarins / Q000737:chemistry; D007202:Indicators and Reagents; D010777:Photochemistry; D012995:Solubility
D000180:Acrylic Resins; D004268:DNA-Binding Proteins; D011564:Furocoumarins; D007202:Indicators and Reagents; C052970:poly-N-isopropylacrylamide; D004247:DNA; D015246:Deoxyribonuclease EcoRI