Double color in situ hybridization of alpha-satellite chromosome 13, 21 specific cosmid clones for a rapid screening of their specificity.
Double color in situ hybridization was used to determine the specificity of cloned alpha-satellite cosmid DNA sequences belonging to satellite DNA sequences shared by chromosomes 13 and 21. It was determined that cosmid clone cos56D8 is more specific to chromosome 13, cosmid clone cos37E is more specific to chromosome 21 and cosmid clones cosA5130 is specific to both chromosomes 13 and 21. These new cosmid probes could bw useful in the molecular-cytogenetic analysis of trisomic cells at Patau or Down syndromes as well as in analysis of marker chromosomes originated from the chromosomes 13 and 21.
Soloviev
I V
IV
National Research Center of Mental Health, RAMS, Moscow.
Yurov YuD
Vorsanova
S G
SG
Malet
P
P
Zerova
T E
TE
Buzhievskaya
T I
TI
D002478:Cells, Cultured; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D003001:Cloning, Molecular; D003116:Color; D003360:Cosmids / Q000235:genetics*; D015342:DNA Probes; D004276:DNA, Satellite / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase / Q000235:genetics; D008677:Metaphase / Q000235:genetics
D015342:DNA Probes; D004276:DNA, Satellite