Colocalization of tumor necrosis factor-alpha and nitric oxide-synthase immunoreactivity in mast cell granules of nasal mucosa.
We have demonstrated, with immunohistochemical techniques, the colocalization of tumour necrosis factor-alpha (TNF alpha) with a constitutive neuronal isoform of nitric oxide-synthase (NOS) in granules of the majority (52.77%) of the mast cells (MCs) of healthy human nasal mucosa. Very few cells were positive for NOS alone (2.54%). Some cells were positive for TNF alpha alone (16.73%) or negative for both antigens (18%). Since dim degranulation occurs in MCs of healthy nasal mucosa at any time, we propose that low concentrations of TNF alpha and NOS secreted by these cells are involved not only in the regulation of homeostasis of normal human nasal mucosa, but also in the survival and function of MCs themselves.
Bacci
S
S
Department of Human Anatomy and Histology, University of Florence, Italy.
Rucci
L
L
Riccardi-Arbi
R
R
Borghi-Cirri
M B
MB
D000293:Adolescent; D000328:Adult; D003594:Cytoplasmic Granules / Q000737:chemistry; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000737:chemistry* / Q000201:enzymology; D009297:Nasal Mucosa / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052248:Nitric Oxide Synthase Type I; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
D014409:Tumor Necrosis Factor-alpha; C496320:NOS1 protein, human; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I