title: Time-lapse microscopy reveals unique roles for kinesins during anaphase in budding yeast.
abstract: The mitotic spindle is a complex and dynamic structure. Genetic analysis in budding yeast has identified two sets of kinesin-like motors, Cin8p and Kip1p, and Kar3p and Kip3p, that have overlapping functions in mitosis. We have studied the role of three of these motors by video microscopy of motor mutants whose microtubules and centromeres were marked with green fluorescent protein. Despite their functional overlap, each motor mutant has a specific defect in mitosis: cin8Delta mutants lack the rapid phase of anaphase B, kip1Delta mutants show defects in the slow phase of anaphase B, and kip3Delta mutants prolong the duration of anaphase to the point at which the spindle becomes longer than the cell. The kip3Delta and kip1Delta mutants affect the duration of anaphase, but cin8Delta does not.
authors.affiliation: Department of Physiology, School of Medicine, University of California San Francisco, San Francisco, California 94143, USA. aaron_straight@hms.harvard.edu
authors.forename: A F
authors.initials: AF
authors.lastname: Straight
authors.forename: J W
authors.initials: JW
authors.lastname: Sedat
authors.forename: A W
authors.initials: AW
authors.lastname: Murray
mesh_terms: D000705:Anaphase / Q000502:physiology*; D005656:Fungal Proteins / Q000378:metabolism; D016547:Kinesins / Q000378:metabolism / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008938:Mitosis; D020409:Molecular Motor Proteins; D016296:Mutagenesis; D010784:Photomicrography; D029701:Saccharomyces cerevisiae Proteins; D004718:Saccharomycetales / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D008941:Spindle Apparatus; D013997:Time Factors
chemical_list: C075342:CIN8 protein, S cerevisiae; D005656:Fungal Proteins; C431747:KIP1 protein, S cerevisiae; C108256:KIP3 protein, S cerevisiae; D008869:Microtubule-Associated Proteins; D020409:Molecular Motor Proteins; D029701:Saccharomyces cerevisiae Proteins; D016547:Kinesins