Do overexpressed oncoproteins cause malignant growth of cancer cells?--Studied by antisense oligonucleotides.
Antisense oligonucleotides were used to demonstrate the importance of insulin-like growth factor II and alpha-fetoprotein for the growth of hepatoma cell lines. The level of insulin-like growth factor II was found to correlate positively with cell proliferative activity, whereas alpha-fetoprotein was not. We have developed an in vitro system for the screening of antisense oligonucleotides effective for inhibiting target protein production. Using this system, the effectiveness of antisense oligonucleotides can be determined even when a specific antibody or activity assay method is not available. These approaches will be useful for verifying the physiological role of other oncoproteins or proteins in living cells, and antisense oligonucleotides may be developed as new therapeutic agents.
Lin
S B
SB
Graduate Institute of Medical Technology, College of Medicine and Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University, Taipei, ROC. sblin@ha.mc.ntu.edu.tw
D002455:Cell Division / Q000235:genetics*; D006801:Humans; D007335:Insulin-Like Growth Factor II / Q000096:biosynthesis; D009369:Neoplasms / Q000473:pathology*; D016376:Oligonucleotides, Antisense; D015513:Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D000509:alpha-Fetoproteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis
D016376:Oligonucleotides, Antisense; D015513:Oncogene Proteins; D000509:alpha-Fetoproteins; D007335:Insulin-Like Growth Factor II