Spectral sensitivity of cones in the goldfish, Carassius auratus.
The spectral sensitivities of retinal cones isolated from goldfish (Carassius auratus) retinas were measured in the range 277-737 nm by recording membrane photocurrents with suction pipette electrodes (SPE). Cones were identified with lambda max (+/- S.D.) at 623 +/- 6.9 nm, 537 +/- 4.7 nm, 447 +/- 7.7 nm, and about 356 nm (three cells). Two cells (lambda max 572 and 576 nm) possibly represent genetic polymorphism. A single A2 template fits the alpha-band of P447(2), P537(2), and P623(2). HPLC analysis showed 4% retinal:96% 3-dehydroretinal. Sensitivity at 280 nm is nearly half that at the lambda max in the visible. The lambda max of the beta-band (in nm) is a linear function of the lambda max of the alpha-band and follows the same relation as found for A1-based cone pigments of a cyprinid fish.
Palacios
A G
AG
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520-8103, USA.
Varela
F J
FJ
Srivastava
R
R
Goldsmith
T H
TH
D000818:Animals; D003118:Color Perception / Q000502:physiology*; D006054:Goldfish / Q000502:physiology*; D066298:In Vitro Techniques; D008564:Membrane Potentials; D010775:Photic Stimulation; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D012168:Retinal Pigments / Q000032:analysis; D013053:Spectrophotometry
D012168:Retinal Pigments