title: Comparative study of tissue reactions to calcium phosphate ceramics among cancellous, cortical, and medullar bone sites in rabbits.
abstract: In order to understand the influence of the implantation site on bone biomaterial evaluation, we implanted cylinders of HA and beta-TCP ceramics in the femoral diaphysis and condyle of rabbits. After 3, 8, 12, and 24 weeks of implantation, histological investigation and histomorphometry were performed on undecalcified samples. Our results show that spontaneous bone healing in the empty cavities is significantly different (p < 0.05) between cortical (SBH > 80%) and cancellous bone sites (SBH < 31%) and that no new bone is formed in marrow tissue. For both porous ceramics, the highest osteogenesis was obtained in the cortical site. Osteogenesis was intermediate in the cancellous site and weak in the medullar site. The material biodegradation was the strongest in the medullar site and higher in the cancellous site than in the cortical site. Both activities were better in the beta-TCP than in the HA (p > 0.05). The marrow tissue presents a foreign-body reaction more reliable, sensitive, and durable than other bone tissues. Therefore, the cancellous bone site is a good site for evaluation of the biofunctionality of biomaterials because of the equilibrium of the osteogenesis and the biodegradation activities, but marrow tissue seems to be better for testing material biocompatibility in vivo.
authors.affiliation: Institut de Recherche sur les Maladies du Squelette, Berck-Sur-Mer, France. irms@wanadoo.fr
authors.forename: J X
authors.initials: JX
authors.lastname: Lu
authors.forename: A
authors.initials: A
authors.lastname: Gallur
authors.forename: B
authors.initials: B
authors.lastname: Flautre
authors.forename: K
authors.initials: K
authors.lastname: Anselme
authors.forename: M
authors.initials: M
authors.lastname: Descamps
authors.forename: B
authors.initials: B
authors.lastname: Thierry
authors.forename: P
authors.initials: P
authors.lastname: Hardouin
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001842:Bone and Bones / Q000502:physiology*; D002130:Calcium Phosphates; D002516:Ceramics; D008855:Microscopy, Electron, Scanning; D011817:Rabbits
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; C485828:alpha-tricalcium phosphate; C485830:tetracalcium phosphate; C485838:calcium phosphate, monobasic, anhydrous; C020243:calcium phosphate; C485829:calcium phosphate, dibasic, anhydrous