title: Ratio of indocyanine green and glucose dilutions detects capillary protein leakage following endotoxin injection in dogs.
abstract: This study was designed to determine whether endotoxin-induced generalized capillary protein leakage can be detected using the ratio of indocyanine green (ICG) and glucose dilutions. Plasma volume determined by the ICG dilution method (Vd-ICG) and the initial distribution volume of glucose (IDVG) were assessed simultaneously before and after either the administration of lipopolysaccharide (LPS) 0.3 mg kg-1 or equal amounts of isotonic saline in two groups of eight dogs, using a one-compartment model after simultaneous infusions of both ICG 0.5 mg kg-1 and glucose 100 mg kg-1. The IDVG has been shown to indicate the extracellular fluid volume of highly perfused organs including plasma. All post-treatment values decreased compared with the pre-treatment values (P < 0.05), except Vd-ICG in the LPS group, even though the post-treatment total plasma protein concentration of the LPS group decreased significantly compared with the pre-treatment value (P < 0.05). The post-treatment Vd-ICG/IDVG ratio of the saline group remained unchanged compared with that of the pre-treatment, and the post-treatment ratio of the LPS group increased significantly (P < 0.01). It can be concluded that overestimation of the Vd-ICG can occur after endotoxin injection, which can be detected by simultaneous measurements of the Vd-ICG and the IDVG.
authors.affiliation: Department of Anaesthesiology, University of Hirosaki School of Medicine, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Sakai
authors.forename: H
authors.initials: H
authors.lastname: Ishihara
authors.forename: T
authors.initials: T
authors.lastname: Iwakawa
authors.forename: A
authors.initials: A
authors.lastname: Suzuki
authors.forename: A
authors.initials: A
authors.lastname: Matsuki
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D019559:Capillary Leak Syndrome / Q000175:diagnosis* / Q000209:etiology / Q000503:physiopathology; D004396:Coloring Agents; D004285:Dogs; D005260:Female; D006439:Hemodynamics; D007208:Indocyanine Green; D008070:Lipopolysaccharides; D008297:Male; D010953:Plasma Volume
chemical_list: D001786:Blood Glucose; D004396:Coloring Agents; D008070:Lipopolysaccharides; D007208:Indocyanine Green