A sequence-specific splicing activator, tra2beta, is up-regulated in response to nerve injury.
Tra2beta is the first mammalian protein which is proved to activate mRNA splicing in sequence-specific manner. Following hypoglossal nerve injury, the expression of Tra2beta mRNA was elevated in injured motoneurons transiently. The up-regulation of Tra2beta mRNA was observed from post-operative day 3 to 21. In addition to the nerve injury in PNS, a brain lesion in CNS also enhanced the expression of Tra2beta mRNA. The present study could be the first observation showing that an expression of the sequence-specific splicing activator is enhanced in neuronal cells in response to nerve injury, and indicates that Tra2beta may participate in the control of injury-specific splicing patterns in order to express molecules which are necessary for regeneration.
Kiryu-Seo
S
S
Department of Anatomy, Asahikawa Medical college, Nishikagura 4-5-3-11, Asahikawa, 078-8510, Japan.
Matsuo
N
N
Wanaka
A
A
Ogawa
S
S
Tohyama
M
M
Kiyama
H
H
D000818:Animals; D019771:Axotomy; D004292:Dominance, Cerebral; D005786:Gene Expression Regulation; D007002:Hypoglossal Nerve / Q000378:metabolism; D061228:Hypoglossal Nerve Injuries; D008297:Male; D008526:Medulla Oblongata / Q000378:metabolism*; D009046:Motor Neurons / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D012326:RNA Splicing; D016601:RNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D000068103:Serine-Arginine Splicing Factors; D013702:Temporal Lobe / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins; C490770:Tra2b protein, rat; D000068103:Serine-Arginine Splicing Factors