Effects of basic fibroblast growth factor on proliferation and phenotype expression of chondrocytes embedded in collagen gel.
1. Whether basic fibroblast growth factor (bFGF) can stimulate proliferation and synthesis of chondroitin sulfate of chondrocytes in gel culture while maintaining the phenotype was studied. 2. At 3 weeks in culture, the cell number in 1.0 ng/ml (18.5+/-2.1 x 10(5)) and 10.0 ng/ml of the bFGF group (15.3+/-1.9 x 10(5)) was significantly larger than that in 0 ng/ml (12.3+/-2.1 x 10(5)), 0.1 ng/ ml (11.7+/-2.2 x 10(5)) and 100.0 ng/ml of the bFGF group (9.8+/-2.3 x 10(5)). 3. All doses of bFGF used in this study suppressed synthesis of chondroitin 6-sulfate. 4. Chondrocyte phenotype in gel culture was maintained for 4 weeks even with stimulation of bFGF.
Matsusaki
M
M
Department of Orthopaedics, Shimane Medical University, Japan.
Ochi
M
M
Uchio
Y
Y
Shu
N
N
Kurioka
H
H
Kawasaki
K
K
Adachi
N
N
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002452:Cell Count / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002809:Chondroitin Sulfates / Q000037:antagonists & inhibitors; D003094:Collagen; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005109:Extracellular Matrix / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005782:Gels; D007150:Immunohistochemistry; D010641:Phenotype; D011817:Rabbits; D016610:Tissue Embedding
D003470:Culture Media; D005782:Gels; D016222:Fibroblast Growth Factor 2; D002809:Chondroitin Sulfates; D003094:Collagen