title: Saccharomyces cerevisiae ACR2 gene encodes an arsenate reductase.
abstract: The ACR2 gene of Saccharomyces cerevisiae was disrupted by insertion of a HIS3 gene. Cells with the disruption were sensitive to arsenate. This phenotype could be complemented by ACR2 on a plasmid. The ACR2 gene was cloned and expressed in Escherichia coli as a malE gene fusion with a C-terminal histidine tag. The combination of chimeric MBP-Acr2-6H protein and yeast cytosol from an ACR2-disrupted strain exhibited arsenate reductase activity.
authors.affiliation: Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA.
authors.forename: R
authors.initials: R
authors.lastname: Mukhopadhyay
authors.forename: B P
authors.initials: BP
authors.lastname: Rosen
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics* / Q000378:metabolism; D053502:Arsenate Reductases; D001149:Arsenates / Q000494:pharmacology; D053501:Arsenite Transporting ATPases; D019677:Artificial Gene Fusion; D004352:Drug Resistance, Microbial / Q000235:genetics; D004926:Escherichia coli; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D016623:Ion Pumps; D009097:Multienzyme Complexes; D010957:Plasmids; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D001149:Arsenates; D016623:Ion Pumps; D009097:Multienzyme Complexes; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; C506971:ARR2 protein, S cerevisiae; D053502:Arsenate Reductases; D000251:Adenosine Triphosphatases; D053501:Arsenite Transporting ATPases