title: Antifungal metabolites (monorden, monocillin IV, and cerebrosides) from Humicola fuscoatra traaen NRRL 22980, a mycoparasite of Aspergillus flavus sclerotia.
abstract: The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a sclerotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zone of inhibition surrounding paper assay disks on agar plates seeded with conidia of A. flavus. Monorden was twice as inhibitory to A. flavus mycelium extension (MIC > 28 microg/ml) as monocillin IV (MIC > 56 microg/ml). Cerebrosides C and D, metabolites known to potentiate the activity of cell wall-active antibiotics, were separated from the ethyl acetate extract but were not inhibitory to A. flavus when tested as pure compounds. This is the first report of natural products from H. fuscoatra.
authors.affiliation: Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, USA. wicklodt@mail.ncaur.usda.gov
authors.forename: D T
authors.initials: DT
authors.lastname: Wicklow
authors.forename: B K
authors.initials: BK
authors.lastname: Joshi
authors.forename: W R
authors.initials: WR
authors.lastname: Gamble
authors.forename: J B
authors.initials: JB
authors.lastname: Gloer
authors.forename: P F
authors.initials: PF
authors.lastname: Dowd
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001203:Ascomycota / Q000737:chemistry* / Q000254:growth & development / Q000302:isolation & purification; D001231:Aspergillus flavus; D002554:Cerebrosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D018942:Macrolides; D008826:Microbial Sensitivity Tests; D012275:Oryza / Q000382:microbiology
chemical_list: D000935:Antifungal Agents; D002554:Cerebrosides; D007783:Lactones; D018942:Macrolides; C116417:monocillin IV; C035359:monorden