title: Performance optimization of the ThinPrep processor: effect of microscope slides.
abstract: Our objective was to evaluate the cellular adherence property of various microscope slides. Slide preparations were made using the ThinPrep 2000 System, in which cells are transferred from a filter to a slide. A total of eight slide types was selected for testing against the ThinPrep microscope slide: S/P MicroSlide, S/P Pusher, S/P Colorfrost, Menzel Superfrost, Sayag, Sail Brand, Fisher Plus, and Newcomer Poly-L-Lysine. Gynecologic samples from 4 different patients were utilized on the ThinPrep Processor. Multiple preparations were made from each sample using the different microscope slides. The slides were stained with a commercial Pap stain, and cellular material was quantified by scanning each preparation using a linear stage mounted on a Hitachi U-2000 spectrophotometer set at 580 nm. The data generated by this study show that ThinPrep microscope slides produce preparations with the highest level of cellular material. This study quantifies slide performance, examines a range of microscope slide types, and shows that ThinPrep microscope slides exhibit significantly increased cell adherence over the other slide types tested.
authors.affiliation: Cytyc Corporation, Boxborough, Massachusetts, USA. dan.lapen@cytyc.com
authors.forename: D C
authors.initials: DC
authors.lastname: Lapen
authors.forename: J
authors.initials: J
authors.lastname: Papillo
mesh_terms: D002448:Cell Adhesion; D002452:Cell Count; D005069:Evaluation Studies as Topic; D005260:Female; D016591:Histocytological Preparation Techniques / Q000592:standards*; D006801:Humans; D008853:Microscopy / Q000295:instrumentation*; D014014:Tissue Adhesives; D014626:Vaginal Smears
chemical_list: D014014:Tissue Adhesives