Protein profile characterization of bacterial lysates by capillary electrophoresis.
A fast and reproducible method was developed to characterize cell lysates by their electrophoretic profiles using capillary electrophoresis (CE). Characteristic and reproducible patterns were recorded for each bacterial strains when "dynamic sieving" CE, using a polymer solution in the capillary, was applied to distinguish four strains of the Enterobacteriaceae family. The electropherograms showed distinct differences when comparing them to the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein profiles. This is certainly a result of the differences in the separation principles and in the detection methods of the two techniques.
Kustos
I
I
Department of Medical Microbiology and Immunology, Medical University of Pécs, Hungary.
Kocsis
B
B
Kerepesi
I
I
Kilár
F
F
D001426:Bacterial Proteins / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000737:chemistry* / Q000254:growth & development; D004926:Escherichia coli / Q000737:chemistry; D011108:Polymers; D011511:Proteus / Q000737:chemistry; D015203:Reproducibility of Results; D012475:Salmonella / Q000737:chemistry; D012764:Shigella sonnei / Q000737:chemistry
D001426:Bacterial Proteins; D011108:Polymers