title: Effect of amino acid cardioplegia on myocardial metabolism and function of ischemic canine heart.
abstract: To evaluate the effect of amino acid cardioplegia on myocardial metabolism and function of ischemic canine heart, canine cardiopulmonary bypass (CPB) model was established and the dog heart was subjected to a 120 min ischemic arrest. Animals were divided into 3 groups, group 1: warm blood cardioplegia induction and terminal perfusion plus 4 C ST. Thomas hospital solution (STS) during ischemia; group 2: warm blood cardioplegia enriched with amino acid (L-asparte and L-glutamate 13 mmol/L each) and STS without amino acid (A. G.) and group 3: both warm blood cardioplegic solution and STS enriched with A. G. . The result demonstrated that the cardiac function of animals in group 2 and 3 had a significantly better recovery after ischemic-re-perfusion. By the end of ischemia the content of myocardial ATP in group 3 was distinctly higher than that in group 1 (P < 0.05), with the release of cardiac enzyme being the least. Myocardial ultra-structure almost remained intact before and after ischemia. Our experiment suggests that the cardioplegia arrest with warm blood and cold crystalloid solution enriched with amino acids could diminish the ischemia-re-perfusion injuries of the heart and enhance the effect of myocardial protection.
authors.affiliation: Institute of Cardiovascular Diseases, Tongji Medical University, Wuhan.
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Lan
authors.forename: G
authors.initials: G
authors.lastname: Cheng
authors.forename: P
authors.initials: P
authors.lastname: Fu
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: Z
authors.initials: Z
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Feng
mesh_terms: D000596:Amino Acids; D000818:Animals; D001224:Aspartic Acid; D001769:Blood; D002314:Cardioplegic Solutions; D004285:Dogs; D018698:Glutamic Acid; D006324:Heart Arrest, Induced; D006358:Hot Temperature; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D002314:Cardioplegic Solutions; D001224:Aspartic Acid; D018698:Glutamic Acid