title: Separation of several free polyamines and their acetylated derivatives by ion-pair reversed-phase high performance liquid chromatography.
abstract: Free polyamines and their acetylated derivatives, [putrescine (Put), spermine (Spm), spermidine (Spd), N1-acetylspermidine N1-AcSpd), N8-acetylspermidine N8-AcSpd)], were separated using ion-pair reversed-phase liquid chromatography after pre-column derivatization with dansyl chloride. Also 1,6-hexanediamine (1,6-Hda) and 1,7-diaminoheptane (1,7-Dah) which used as an internal standards were separated under the same chromatographic conditions. The reported method provided a good resolution between N1-AcSpd and N8 AcSpd (Rs = 0.86). This quality of resolution together with the very low detection limit obtained, (33.6, 8.33, 2.72, 2.67, 4.76, 4.27, and 2.27 fmoles/microliters for N1-AcSpd, N8-AcSpd, Put, 1,6-Hda, 1,7-Dah, Spm, and Spd, respectively), offer advantages to this method. Furthermore, a significant correlation coefficients were obtained as follows: 0.998 (N8-AcSpd), 0.996 N1-AcSpd), 0.997 (Put), 0.9995 (1,6-Hda), 0.9992 (1,7-Dah), 0.9996 (Spd), and 0.997 (Spm). The described method can be adapted for the separation of the free and acetylated polyamines in biological specimens.
authors.affiliation: Biological and Medical Research Department (MBC 03), King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia.
authors.forename: H Y
authors.initials: HY
authors.lastname: Aboul-Enein
authors.forename: I A
authors.initials: IA
authors.lastname: al-Duraibi
mesh_terms: D000107:Acetylation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007477:Ions; D011073:Polyamines / Q000737:chemistry / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
chemical_list: D007477:Ions; D011073:Polyamines