Growth hormone-releasing peptide-2 (GHRP-2) does not act via the human growth hormone-releasing factor receptor in GC cells.
Effect of growth hormone-releasing peptide-2 (GHRP-2) on ovine somatotrophs is abolished by a growth hormone-releasing factor (GRF) receptor antagonist, which raises the possibility that GHRP-2 may act on GRF receptors. In the present study, we used rat pituitary GC cells with or without stable transfection of cDNA coding for the human GRF receptor (GC/R+ or GC/R-) to determine whether or not GHRP-2 acts via the GRF receptor. Northern blot analysis indicated that GRF receptor mRNA was undetectable in GC/R-cells, whereas a high level of expression occurred in GC/R+ cells that were transfected by GRF receptor cDNA. In GC/R- cells, incubation with up to 10(-7)M of either hGRF or GHRP-2 did not alter the intracellular cAMP, [Ca2+]i, or GH secretion. In GC/R+ cells, hGRF (10(-11)-10(-7)M) increased cAMP levels in a concentration-dependent manner up to 20-fold. This increase in cAMP levels was blocked by a GRF receptor antagonist, [Ac-Tyr1, D-Arg2]-GRF 1-29, but not by a Ca2+ channel blocker, NiCl2 (0.5 mM). GH secretion and [Ca2+]i were, however, not increased by hGRF. Incubation of the transfected cells with 10(-1)-10(-8)MGH RP-2 did not modify intracellular cAMP levels. This result suggests that GHRP-2 does not act through the GRF receptor.
Chen
C
C
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia. chen.chen@med.monash.edu.au
Farnworth
P
P
Petersenn
S
S
Musgrave
I
I
Canny
B J
BJ
Clarke
I J
IJ
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D013007:Growth Hormone-Releasing Hormone / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000494:pharmacology*; D010902:Pituitary Gland / Q000502:physiology*; D051381:Rats; D018013:Receptors, Neuropeptide / Q000819:agonists / Q000502:physiology*; D018017:Receptors, Pituitary Hormone-Regulating Hormone / Q000819:agonists / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D014162:Transfection
D009842:Oligopeptides; D018013:Receptors, Neuropeptide; D018017:Receptors, Pituitary Hormone-Regulating Hormone; D013007:Growth Hormone-Releasing Hormone; C091874:growth hormone-releasing peptide-2; C048250:somatotropin releasing hormone receptor