[Standardization of hemopoietic colony assay reagents].
Several Japanese Red Cross blood centers have begun cooperating with hospitals in peripheral blood stem cell transplantation research. However, most have not yet standardized their techniques or reagents for that purpose yet. Consequently, wide variations are often observed in data from different blood centers, especially for hemopoietic colony assays. We compared our colony assay reagent set with those in three commercial colony assay kits. The best results were obtained with the kit from a manufacturer referred to here as company A. Although our reagent set obtained lower colony values, the CFU-GM, BFU-E, and total colony values correlated well with those obtained using company A's kit (r = 0.74, 0.80, and 0.97, respectively). Company A's kit gave reproducible results even with the use of different lots, and includes reagents that can be stored for up to two years at -20 degrees C. These features highlighted its advantages as a standard reagent set.
Ueda
M
M
Japanese Red Cross Central Blood Center.
Takanashi
M
M
Tsubokura
M
M
Ide
T
T
Iwai
S
S
Okai
M
M
Akaza
T
T
Nakajima
K
K
Tadokoro
K
K
Juji
T
T
D003114:Colony-Forming Units Assay / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D011933:Reagent Kits, Diagnostic / Q000592:standards*
D011933:Reagent Kits, Diagnostic