title: Increased pulmonary blood flow produces endothelial cell dysfunction in neonatal swine.
abstract: BACKGROUND
  The mechanisms by which increased pulmonary blood flow results in pulmonary hypertension have not been determined.
  
  
  METHODS
  To determine if increased pulmonary blood flow produces endothelial dysfunction that precedes vascular remodeling and smooth muscle proliferation, neonatal swine (n = 12) (age, 6.1+/-0.5 days) underwent ligation of the left pulmonary artery (LPA) to increase blood flow to the right lung. At 12 weeks of age, endothelium-dependent vasodilatation was assessed by acetylcholine infusion and endothelium-independent vasodilatation by inhaled nitric oxide (NO) in the LPA group and age-matched controls (CON) (n = 11).
  
  
  RESULTS
  Mean pulmonary artery pressure was 24.1+/-3.0 mm Hg in the LPA group and 20.8+/-1.9 mm Hg in the CON group (p < 0.1). Pulmonary vascular resistance was 13.2+/-2.2 Wood units in the LPA group and 5.8+/-0.8 Wood units in the CON group (p = 0.001). Acute occlusion of the left pulmonary artery in the CON group increased pulmonary vascular resistance to 6.9+/-3.9 Wood units (p = 0.04). Administration of acetylcholine in the CON group after preconstriction with the thromboxane A2 analogue U46619 resulted in a 30.6%+/-5.4% decrease in pulmonary vascular resistance. In the LPA group, acetylcholine produced paradoxical vasoconstriction and a 15.4%+/-4.1% increase in pulmonary vascular resistance (p < 0.001 versus CON) indicating loss of endothelium-dependent vasodilatation. Nitric oxide decreased pulmonary vascular resistance by 41.9%+/-3.3% in the CON group and 30.8%+/-2.7% in the LPA group (p = 0.04 versus CON), indicating preserved endothelium-independent vasodilatation in both groups. Morphometric analysis was performed in 4 animals from each group. Medial wall thickness as percent of external diameter of small arteries (<100 microm) was the same in both groups (6.4%+/-0.4% in the LPA group versus 6.6% +/-0.4% in the CON animals; p > 0.1).
  
  
  CONCLUSIONS
  Increased pulmonary blood flow in immature animals produces endothelial cell dysfunction with loss of endothelium-dependent vasodilatation before the onset of pulmonary vascular remodeling. Subsequent smooth muscle proliferation may be mediated by endothelium-derived factors.
authors.affiliation: Department of Pathology, The Children's Hospital of Philadelphia, Pennsylvania 19104, USA.
authors.forename: E V
authors.initials: EV
authors.lastname: Vitvitsky
authors.forename: J P
authors.initials: JP
authors.lastname: Griffin
authors.forename: M H
authors.initials: MH
authors.lastname: Collins
authors.forename: T L
authors.initials: TL
authors.lastname: Spray
authors.forename: J W
authors.initials: JW
authors.lastname: Gaynor
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D004730:Endothelium, Vascular / Q000166:cytology / Q000503:physiopathology*; D006976:Hypertension, Pulmonary / Q000209:etiology*; D008168:Lung / Q000098:blood supply / Q000254:growth & development; D009131:Muscle, Smooth, Vascular / Q000166:cytology; D009569:Nitric Oxide / Q000502:physiology; D011651:Pulmonary Artery / Q000473:pathology / Q000503:physiopathology*; D011652:Pulmonary Circulation / Q000502:physiology*; D013552:Swine; D014655:Vascular Resistance / Q000502:physiology; D014664:Vasodilation / Q000502:physiology
chemical_list: D009569:Nitric Oxide