title: Effects of beta-lactoglobulin on the tight-junctional stability of Caco-2-SF monolayer.
abstract: The mechanisms for tight-junction (TJ) stabilization by beta-lactoglobulin (beta-Lg) were studied. Treatment of Caco-2-SF cells with inhibitors for some enzymes in the intracellular signal transduction pathways and a cytoskeleton-disturbing agent (cytochalasin D) reduced the TJ-stabilizing activity of beta-Lg. So beta-Lg is suggested to modulate the cytoskeletal structure through the activation of phospholipase C and protein kinase C, resulting in the TJ stabilization.
authors.affiliation: Department of Bioproductive Science, Utsunomiya University, Japan. keih@cc.utsunomiya-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Hashimoto
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.forename: M
authors.initials: M
authors.lastname: Shimizu
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D006801:Humans; D007782:Lactoglobulins / Q000378:metabolism*; D019108:Tight Junctions / Q000502:physiology*
chemical_list: D007782:Lactoglobulins