title: Mucosal antibody expression following rapid SIV(Mne) dissemination in intrarectally infected Macaca nemestrina.
abstract: The early kinetics of antibody expression following transmucosal infection by SIV(Mne) were examined in several mucosal compartments in Macaca nemestrina. Five male-female pairs of macaques were inoculated intrarectally with SIV(Mne) E11S, a biological clone, and serially euthanized at 1, 2, 4, 8, and 12 weeks postinoculation. Plasma, tears, saliva, rectal secretions, and vaginal washes were collected serially and just prior to euthanasia. Both total and SIV-specific IgG and IgA levels were measured by immunoglobulin isotype-specific quantitative enzyme-linked immunosorbent assays (ELISAs), and were further examined by conventional and enhanced chemiluminescence (ECL) immunoblots. Virus coculture, polymerase chain reaction, and in situ hybridization assays revealed the systemic spread of virus as early as 1 week postinoculation in 8 of 10 animals. ECL immunoblots detected SIV-specific antibodies in mucosal samples collected 1 week postinoculation. The most dramatic increases in both total and SIV-specific IgA levels were detected in rectal secretion samples. In contrast, plasma and nonrectal mucosal samples from the same time points increased only slightly, suggesting that the most robust antibody response occurred at the portal of infection. Our results show that the SIV-infected macaque is an excellent model for studies designed to assess mucosal immune responses to primate lentivirus infections. Additional studies will assess the correlation between the antiviral protection afforded by candidate vaccines and mucosal antibody responses.
authors.affiliation: Washington Regional Primate Research Center, University of Washington, Seattle 98195-7330, USA.
authors.forename: L
authors.initials: L
authors.lastname: Kuller
authors.forename: J
authors.initials: J
authors.lastname: Thompson
authors.forename: R
authors.initials: R
authors.lastname: Watanabe
authors.forename: D
authors.initials: D
authors.lastname: Iskandriati
authors.forename: C E
authors.initials: CE
authors.lastname: Alpers
authors.forename: W R
authors.initials: WR
authors.lastname: Morton
authors.forename: M B
authors.initials: MB
authors.lastname: Agy
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000956:Antigens, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D018928:Immunity, Mucosal; D015151:Immunoblotting; D007132:Immunoglobulin Isotypes / Q000276:immunology; D008254:Macaca nemestrina; D008297:Male; D018448:Models, Immunological; D009894:Opportunistic Infections / Q000276:immunology; D012007:Rectum; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D007132:Immunoglobulin Isotypes