title: Detection of the satA gene and transferability of virginiamycin resistance in Enterococcus faecium from food-animals.
abstract: The satA gene encoding streptogramin A resistance was detected in virginiamycin-resistant Enterococcus faecium isolates from pigs and broilers. The satA gene was present in 22 of 89 (25%) virginiamycin-resistant E. faecium isolates. It was shown that the satA gene and other gene(s) encoding streptogramin resistance could be transferred between isogenic E. faecium strains at frequencies ranging from 2.3 x 10(-4) to 2.2 x 10(-3) transconjugants per donor.
authors.affiliation: Danish Veterinary Laboratory, Copenhagen V. Denmark. aha@svs.dk
authors.forename: A M
authors.initials: AM
authors.lastname: Hammerum
authors.forename: L B
authors.initials: LB
authors.lastname: Jensen
authors.forename: F M
authors.initials: FM
authors.lastname: Aarestrup
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins; D001483:Base Sequence; D002645:Chickens / Q000382:microbiology; D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial / Q000235:genetics*; D004586:Electrophoresis; D016521:Electrophoresis, Gel, Pulsed-Field; D016984:Enterococcus faecium / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D007422:Intestines / Q000382:microbiology; D008460:Meat / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA; D013552:Swine / Q000382:microbiology; D014769:Virginiamycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D014769:Virginiamycin; D000123:Acetyltransferases; C084756:streptogramin A acetyltransferase, Enterococcus faecium