Differences in enterocyte brush border enzyme activities in ageing rats reared in germ-free and conventional conditions.
The aim of this study was to evaluate the levels of disaccharidase and dipeptidyl peptidase i.v. activities in rat jejunal enterocytes under the influence of long-term germ-free conditions. We found that the brush-border lactase and dipeptidyl peptidase i.v. activities were two to three times higher in 2-month-old germ-free rats in comparison with their conventional counterparts. The highest effect of germ-free condition was observed on lactase activity in 6-month-old and dipeptidyl peptidase i.v. in 2-month-old rats. No difference between germ-free and conventional rats in sucrase and glucoamylase activities was found in 2-month-old rats. The difference develops with increasing age, sucrase activity becoming significantly higher in 6- and 12-month-old rats and glucoamylase in 12-month-old germ-free rats.
Kozáková
H
H
Department of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Stĕpánková
R
R
Reháková
Z
Z
Kolínská
J
J
D000375:Aging; D000818:Animals; D018819:Dipeptidyl Peptidase 4 / Q000378:metabolism; D004186:Disaccharidases / Q000378:metabolism; D005260:Female; D005856:Germ-Free Life; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000648:ultrastructure; D007583:Jejunum / Q000201:enzymology* / Q000648:ultrastructure; D043322:Lactase; D008297:Male; D008871:Microvilli / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013393:Sucrase / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism
D004186:Disaccharidases; D043322:Lactase; D001616:beta-Galactosidase; D005087:Glucan 1,4-alpha-Glucosidase; D013393:Sucrase; D018819:Dipeptidyl Peptidase 4