Role of outer membrane proteins on the adherence of Vibrio parahaemolyticus to rabbit intestinal epithelial cell in vitro.
Antiserum against outer membrane preparation of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus were raised in rabbits and absorbed with their lipopolysaccharide. The anti-outer membrane protein serum and its Fab (IgG) fragment inhibited the adherence of Kanagawa-positive strains to rabbit intestinal epithelial cells in vitro. Preincubation of rabbit intestinal epithelial cells with outer membrane preparation also inhibited the adherence of these bacteria. Anti-lipopolysaccharide serum or its Fab (IgG) fragment did not inhibit adherence of V. parahaemolyticus. Moreover, pre-treatment of rabbit intestinal epithelial cells with lipopolysaccharide did not inhibit adherence of these strains. These results suggest that outer membrane proteins of V. parahaemolyticus play an important role in the adherence of Kanagawa-positive strains to rabbit intestinal epithelial cells.
Chakrabarti
M K
MK
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
Bhattacharya
J
J
Sinha
A K
AK
De
S P
SP
D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D004847:Epithelial Cells / Q000382:microbiology*; D011817:Rabbits; D014736:Vibrio parahaemolyticus / Q000378:metabolism*
D001425:Bacterial Outer Membrane Proteins