title: DNA vaccination with HIV-1 expressing constructs elicits immune responses in humans.
abstract: Humoral and cellular immune responses have been produced by intramuscular vaccination with DNA plasmids expressing HIV-1 genes, suggesting possible immunotherapeutic and prophylactic value for these constructs. Vaccination with these constructs has decreased HIV-1 viral load in HIV-1-infected chimpanzees. In addition, naive (i.e. non-HIV-1-infected) chimpanzees were protected against a heterologous challenge with HIV-1. Ongoing phase I clinical trials show that therapeutic vaccinations indeed boost anti-HIV-1 immune responses in humans. A therapeutic phase I trial on humans with these constructs induced a good safety profile and also demonstrated an immunological potentiation. These findings indicate that further studies with these constructs in humans are warranted.
authors.affiliation: University of South Florida College of Medicine, Department of Medical Microbiology and Immunology, Tampa 33612, USA. kugen@com1.med.usf.edu
authors.forename: K E
authors.initials: KE
authors.lastname: Ugen
authors.forename: S B
authors.initials: SB
authors.lastname: Nyland
authors.forename: J D
authors.initials: JD
authors.lastname: Boyer
authors.forename: C
authors.initials: C
authors.lastname: Vidal
authors.forename: L
authors.initials: L
authors.lastname: Lera
authors.forename: S
authors.initials: S
authors.lastname: Rasheid
authors.forename: M
authors.initials: M
authors.lastname: Chattergoon
authors.forename: M L
authors.initials: ML
authors.lastname: Bagarazzi
authors.forename: R
authors.initials: R
authors.lastname: Ciccarelli
authors.forename: T
authors.initials: T
authors.lastname: Higgins
authors.forename: Y
authors.initials: Y
authors.lastname: Baine
authors.forename: R
authors.initials: R
authors.lastname: Ginsberg
authors.forename: R R
authors.initials: RR
authors.lastname: Macgregor
authors.forename: D B
authors.initials: DB
authors.lastname: Weiner
mesh_terms: D016915:AIDS Vaccines / Q000627:therapeutic use*; D000595:Amino Acid Sequence; D000917:Antibody Formation / Q000276:immunology; D004279:DNA, Viral / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015483:HIV Antibodies / Q000096:biosynthesis / Q000097:blood; D015699:HIV Envelope Protein gp120 / Q000276:immunology; D015658:HIV Infections / Q000097:blood / Q000276:immunology / Q000628:therapy*; D015497:HIV-1 / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010957:Plasmids / Q000008:administration & dosage; D019444:Vaccines, DNA / Q000627:therapeutic use*
chemical_list: D016915:AIDS Vaccines; D004279:DNA, Viral; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120; D019444:Vaccines, DNA