hAG-2, the human homologue of the Xenopus laevis cement gland gene XAG-2, is coexpressed with estrogen receptor in breast cancer cell lines.
hAG-2, the human homologue of the Xenopus laevis cement gland gene Xenopus Anterior Gradient-2 (XAG-2), has been found to be coexpressed with estrogen receptor (ER) in breast cancer cell lines by using suppression subtractive hybridization. Sequence analysis of clones obtained from a human breast adenocarcinoma MCF7 cDNA library suggests that at least three mRNA transcripts, differing in their 3'-untranslated regions, are transcribed from the hAG-2 gene. Northern blot analyses identified two principal hAG-2 signals in all ER-positive breast carcinoma cell lines that were absent in cell lines not expressing, or minimally expressing, ER. Coexpression of hAG-2 with ER suggests that hAG-2 may be involved in the tumor biology specific to the well-differentiated phenotype of hormonally-responsive breast cancers.
Thompson
D A
DA
Department of Surgery, Stanford University, Stanford, California, 94305-5494, USA.
Weigel
R J
RJ
D000230:Adenocarcinoma; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001943:Breast Neoplasms / Q000378:metabolism*; D003001:Cloning, Molecular; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D014176:Protein Biosynthesis; D019704:Protein Disulfide-Isomerases; D011506:Proteins / Q000235:genetics*; D011960:Receptors, Estrogen / Q000096:biosynthesis* / Q000235:genetics; D017422:Sequence Analysis, DNA; D014407:Tumor Cells, Cultured; D029867:Xenopus Proteins; D014982:Xenopus laevis
D011506:Proteins; D011960:Receptors, Estrogen; D029867:Xenopus Proteins; D019704:Protein Disulfide-Isomerases; C113451:XAG-2 protein, Xenopus