title: A novel strategy for unambiguous DPB1 typing.
abstract: A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8-9 and 84-87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.
authors.affiliation: Department of Pathology, Royal Melbourne Hospital, Parkville, VIC, Australia.
authors.forename: M D
authors.initials: MD
authors.lastname: Varney
authors.forename: B D
authors.initials: BD
authors.lastname: Tait
mesh_terms: D006682:HLA-DP Antigens / Q000145:classification / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen