title: Screening pregnant women for group B streptococcal colonization.
abstract: The recovery rates of group B streptococcus (GBS) from anorectal swabs (RS) and vaginal swabs (VS) that were enriched were compared to the routine method to determine the optimal procedure. Separate RS and VS were collected from women attending antenatal clinics. RS and VS were placed in 2 ml enrichment and selective broth. Swabs were inoculated onto colistin/nalidixic acid agar (CNA) upon arrival in the laboratory and onto 5% sheep blood agar (SBA) and CNA after 24 h enrichment. The routine method consisted of a VS sent in transport medium and inoculated in the laboratory onto SBA (no enrichment). The overall GBS colonization rate was 24% (64/264). Of the 64 GBS carriers, 77% were colonized in the vagina and 89% were colonized in the anorectum. The anorectum was the only site of colonization in 24% of the women, whereas the vagina was the only site of colonization in 11% of cases. Enrichment increased the detection of GBS from both RS (55 versus 42; P < 0.025) and from VS (49 versus 27; P < 0.001). Of the 64 cases, enriched RS detected 86%, enriched VS detected 77% and the standard VS detected only 41%. Enriched RS and enriched VS collectively detected 99% of cases. SBA was better than CNA for subculture of the enrichment broth because of a higher recovery rate (98-100% versus 80-82%; P < 0.01) and the fact that the hemolysis on SBA made it easier to differentiate GBS from enterococci. The data confirm that optimal screening of pregnant women for GBS should include a combined RS/VS swab placed in enrichment broth that is then subcultured onto SBA after 24 h incubation.
authors.affiliation: Dept. of Medicine, King Abdulaziz University Hospital, Jeddah, Saudi Arabia.
authors.forename: T A
authors.initials: TA
authors.lastname: Madani
authors.forename: G K
authors.initials: GK
authors.lastname: Harding
authors.forename: M
authors.initials: M
authors.lastname: Helewa
authors.forename: M J
authors.initials: MJ
authors.lastname: Alfa
mesh_terms: D003470:Culture Media; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control; D008403:Mass Screening / Q000379:methods*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*; D011295:Prenatal Care; D011446:Prospective Studies; D012007:Rectum / Q000382:microbiology; D013048:Specimen Handling / Q000379:methods; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000517:prevention & control / Q000635:transmission; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology
chemical_list: D003470:Culture Media