The influence of plasma lipoprotein subfractions on 3T3-L1 and human preadipocyte differentiation in cell culture.
3T3-L1 and human preadipocyte differentiation was significantly (P < 0.001) enhanced by HDL2, LDLII/III and LDLIV. The concentrations of lipoproteins required for maximal differentiation in human preadipocytes were not achieved over the concentration range 50-150 micrograms lipoprotein protein ml-1, whereas maximal differentiation in 3T3-L1 preadipocytes was achieved for all lipoprotein subfractions at approximately 75 micrograms lipoprotein ml-1, a level almost double that required for complete HDL and LDL fractions in 3T3-L1 cells. Despite the enhanced extent of differentiation caused by certain lipoprotein subfractions, the time needed for the conversion process was unaffected. GPDH activity development in both cell types was most pronounced in response to LDLIV, with HDL2 resulting in the lowest activity. In both cell types, the enhancement of differentiation was only evident when the cells were exposed to lipoproteins during the early stage of the program, i.e. before visible formation of lipid droplets.
Stanton
L A
LA
Department of Biochemistry and Microbiology, University of Port Elizabeth, South Africa.
van de Venter
M
M
Oelofsen
W
W
D016475:3T3 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D000818:Animals; D015415:Biomarkers; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000145:classification / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000097:blood / Q000494:pharmacology; D053439:Lipoproteins, HDL2; D008077:Lipoproteins, LDL / Q000097:blood / Q000145:classification / Q000494:pharmacology; D051379:Mice; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology
D015415:Biomarkers; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D053439:Lipoproteins, HDL2; D008077:Lipoproteins, LDL; D005993:Glycerolphosphate Dehydrogenase