High-performance thin-layer chromatographic separation of ranitidine hydrochloride and two related compounds.
Ranitidine hydrochloride and its two related compounds, used in the USP TLC purity testing of the drug, were separated on a high-performance thin-layer chromatography (HPTLC) RP-18 WF254S precoated plate using methanol-3% NH4OH (4:1, v/v) as the mobile phase. The main advantage of the proposed HPTLC system over the USP TLC system for testing the purity of ranitidine is a better and more efficient separation of these three compounds in a shorter time and with less consumption of solvents. The system is promising from the point of view of the development of a new method for the TLC purity testing of ranitidine hydrochloride. A video system was used for imaging thin-layer chromatograms. Direct UV densitometric quantitation of the three compounds and a model for the calculation of analytical performance parameters is presented in the second part of the paper.
Simonovska
B
B
National Institute of Chemistry, Ljubljana, Slovenia.
Prosek
M
M
Vovk
I
I
Jelen-Zmitek
A
A
D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry; D007091:Image Processing, Computer-Assisted; D011899:Ranitidine / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D011899:Ranitidine