Molecular cloning and expression of rat contraception associated protein 1 (CAP1), a protein putatively involved in fertilization.
Epididymal fluid from rats rendered infertile by oral administration of ornidazole contains a protein CAP1 (contraception-associated protein 1) that is absent from epididymal fluid, but present on epididymal sperm, from fertile vehicle-treated rats. The gene for CAP1 has been isolated from a rat testis cDNA library and its expression investigated in different tissues. The deduced protein sequence of CAP1 contains 189 amino-acid residues and database searches revealed a high degree of homology (83-95%) with human and mouse DJ-1 at the nucleotide and amino acid levels. Northern blot hybridisation from different rat tissues indicated that CAP1 is encoded by a 1.6-kilobase RNA transcript and seems to be ubiquitiously expressed in the rat with a high level of expression in the testis.
Wagenfeld
A
A
Institute of Reproductive Medicine of the University, Münster, D-48129, Germany.
Gromoll
J
J
Cooper
T G
TG
D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004822:Epididymis / Q000502:physiology*; D005298:Fertility; D005306:Fertilization; D015723:Gene Library; D006801:Humans; D007248:Infertility, Male / Q000139:chemically induced / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000737:chemistry; D009928:Organ Specificity; D009950:Ornidazole / Q000494:pharmacology; D014176:Protein Biosynthesis; D000071617:Protein Deglycase DJ-1; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D013094:Spermatozoa / Q000378:metabolism*
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D011506:Proteins; D011994:Recombinant Proteins; C115438:contraception associated protein 1; D009950:Ornidazole; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1