Neurotransmitter release from semi-intact synaptosomes.
We have developed a secretion assay composed of semi-intact synaptosomes from which transmitter release is optimally evoked by micromolar Ca2+ in the presence of cytosol. Transmitter release from this preparation reconstitutes known characteristics of regulated exocytosis and is accompanied by a marked decrease in synaptic vesicles. The assay is useful in characterizing the components known to be involved in transmitter release, and should also facilitate the identification of additional factors that are important for this process.
Tandon
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A
Departments of Cell and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California, 92037, USA.
Tan
P K
PK
Bannykh
S
S
Banerjee
A
A
Balch
W E
WE
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001905:Botulinum Toxins / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D005089:Exocytosis; D005971:Glutamates / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure
D005971:Glutamates; D018377:Neurotransmitter Agents; D001905:Botulinum Toxins; D002118:Calcium; D009638:Norepinephrine