title: Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography.
abstract: The oxalate oxidase enzyme expressed in barley roots is a thermostable, protease-resistant enzyme that generates H2O2. It has great medical importance because of its use to assay plasma and urinary oxalate, and it has also been used to generate transgenic, pathogen-resistant crops. This protein has now been purified and three types of crystals grown. X-ray analysis shows that the symmetry present in these crystals is consistent with a hexameric arrangement of subunits, probably a trimer of dimers. This structure may be similar to that found in the related seed storage proteins.
authors.affiliation: School of Plant Sciences, The University of Reading, Whiteknights, UK.
authors.forename: E J
authors.initials: EJ
authors.lastname: Woo
authors.forename: J M
authors.initials: JM
authors.lastname: Dunwell
authors.forename: P W
authors.initials: PW
authors.lastname: Goodenough
authors.forename: R W
authors.initials: RW
authors.lastname: Pickersgill
mesh_terms: D018360:Crystallography, X-Ray; D001467:Hordeum / Q000201:enzymology*; D010088:Oxidoreductases / Q000737:chemistry*
chemical_list: D010088:Oxidoreductases; C020598:oxalate oxidase