The guinea pig cochlear AE2 anion exchanger: cDNA cloning and in situ localization within the cochlea.
This study has characterized the repertoire of the anion exchanger (AE) family members expressed within the guinea pig organ of Corti, the auditory neuroepithelia. Both AE2 and AE3 cDNAs were present, but AE1 cDNA was not detected. The more abundant AE2 was sequenced and its expression characterized in the cochlea. The 3888 base pairs (bp) AE2 sequence, compiled from multiple clones, includes 150 bp of upstream non-coding sequence and 3717 bp of open reading frame encoding a protein of 1238 amino acids. Immunoblot of cochlear homogenate revealed a single AE2-immunoreactive band of Mr 180 kDa. In situ hybridization and immunohistochemical analysis localized AE2 expression to several tissues and cell types within the guinea pig inner ear, including superior half of the spiral ligament and within the interdental cells lining the spiral limbus. However, AE2 was not clearly detected in the outer hair cells (OHC) of the organ of Corti by either immunohistochemistry or in situ hybridization. The results of these studies imply a physiologic role of AE2 in the cochlear homeostasis, but do not support its role as a potential 'motor protein' in mediating the in vitro-observed voltage-gated, ATP-independent OHC motility.
Mhatre
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Laboratory of Molecular Otology, Epstein Laboratories, Department of Otolaryngology-Head and Neck Surgery, University of California San Francisco, 533 Parnassus Avenue, U490A, San Francisco, CA 94117, USA.
Charachon
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Alper
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Lalwani
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D000595:Amino Acid Sequence; D000818:Animals; D027321:Anion Transport Proteins; D017920:Antiporters; D001483:Base Sequence; D003001:Cloning, Molecular; D003051:Cochlea / Q000378:metabolism*; D018076:DNA, Complementary / Q000032:analysis / Q000096:biosynthesis*; D006168:Guinea Pigs; D015151:Immunoblotting; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009925:Organ of Corti / Q000378:metabolism; D064606:SLC4A Proteins; D016415:Sequence Alignment
D027321:Anion Transport Proteins; D017920:Antiporters; D018076:DNA, Complementary; D008565:Membrane Proteins; D064606:SLC4A Proteins