Multiple processing of procathepsin L to cathepsin L in vivo.
Three amino-terminal-specific peptidic antibodies against cathepsin L were generated. These antibodies recognize in vitro processing products of procathepsin L in time-course-dependent fashion. Immunoblot analyses with these antibodies followed by immunoprecipitation with anti-cathepsin L antibody showed that the amino terminal amino acid sequences of intracellular cathepsin L are heterogeneous: the single chain form of cathepsin L starts with either EPLML, LKIPK or IPKSV, and the heavy chain of the two chain form with IPKSV. Percoll density gradient and fluorescence immunohistochemistry suggested that these three species of cathepsin L localize in the lysosomes where procathepsin L processing occurs.
Ishidoh
K
K
Department of Biochemistry, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113, Japan. kishidoh@med.Juntendo.ac.jp
Saido
T C
TC
Kawashima
S
S
Hirose
M
M
Watanabe
S
S
Sato
N
N
Kominami
E
E
D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D056668:Cathepsin L; D002403:Cathepsins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D003546:Cysteine Endopeptidases; D010450:Endopeptidases; D004792:Enzyme Precursors / Q000737:chemistry / Q000378:metabolism*; D011905:Genes, ras; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D011499:Protein Processing, Post-Translational; D012867:Skin / Q000201:enzymology
D004792:Enzyme Precursors; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; C063905:procathepsin L; D056668:Cathepsin L; C534278:Ctsl protein, mouse