The porcine proteasome subunit A4 (PSMA4) gene: isolation of a partial cDNA, linkage and physical mapping.
A partial cDNA clone encoding the porcine proteasome subunit A4 (PSMA4 or proteasome subunit C9) has been isolated from a porcine muscle cDNA library and sequenced. A biallelic TaqI RFLP was identified in Large White, Landrace and Duroc breeds. Moreover, the 3'-untranslated region of the gene showed a triallelic SSCP. By linkage analysis the PSMA4 locus was assigned to pig chromosome 7 and by radioactive in situ hybridization this locus was mapped to the region 7q13-q14.
Davoli
R
R
DIPROVAL, Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy.
Fontanesi
L
L
Russo
V
V
Cepica
S
S
Musilová
P
P
Stratil
A
A
Rubes
J
J
D020413:3' Untranslated Regions; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D005787:Gene Frequency; D008040:Genetic Linkage; D006801:Humans; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics*; D020161:Physical Chromosome Mapping; D018807:Polymorphism, Single-Stranded Conformational; D046988:Proteasome Endopeptidase Complex; D011487:Protein Conformation; D051381:Rats; D013045:Species Specificity; D013552:Swine / Q000235:genetics*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D009097:Multienzyme Complexes; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex