Determination of hepatotoxic pyrrolizidine alkaloids by on-line high performance liquid chromatography mass spectrometry with an electrospray interface.
The present study describes the determination of two different types of hepatotoxic pyrrolizidine alkaloids (PAs) and also distinguishing the hepatotoxic PAs from non-toxic ones by both in-source collision-induced dissociation high performance liquid chromatography mass spectrometry (CID-HPLC/MS) and HPLC/MS/MS (CID in the collision cell), using electrospray ionization. The mass spectra provided molecular ions and characteristic fragment ions, which could be used readily for a rapid identification of different types of PAs. Applications of both in-source CID-HPLC/MS and HPLC/MS/MS analytical methods were successful for the determination of PAs in blood samples obtained from rats dosed with PAs and in the PA-containing plant. The results demonstrated that the developed HPLC/MS methods with two different CID techniques provided a very simple and rapid analysis for an unequivocal diagnosis of PA poisoning and for definitive identification of PAs in plants or herbal medicines.
Lin
G
G
Department of Pharmacology, Chinese University of Hong Kong, Shatin, SAR.
Zhou
K Y
KY
Zhao
X G
XG
Wang
Z T
ZT
But
P P
PP
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D008297:Male; D013058:Mass Spectrometry; D010944:Plants / Q000737:chemistry; D011763:Pyrrolizidine Alkaloids / Q000032:analysis* / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
D011763:Pyrrolizidine Alkaloids