Flavin-containing monooxygenase mediated metabolism of benzydamine in perfused brain and liver.
Benzydamine (BZY) N-oxidation mediated by flavin-containing monooxygenase (FMO) was evaluated in perfused brain and liver. Following 20 min of perfusion with modified Ringer solution, the infusion of BZY into brain or liver led to production of BZY N-oxide. BZY N-oxide, a metabolite of BZY oxidized exclusively by FMO, was mostly recovered in the effluent without undergoing further metabolism or reduction back to the parent substrate. The BZY N-oxide formation rate increased as the infusion concentration of BZY increased both in perfused brain and perfused liver. BZY N-oxidation activities in perfused rat brain and liver were 4.2 nmol/g brain/min and 50 nmol/g liver/min, respectively, although the BZY N-oxidation activity in brain homogenates was one 4000th that in liver homogenates. This is the first study of FMO activity in brain in situ.
Kawaji
A
A
Department of Toxicology, Faculty of Pharmaceutical Sciences, Setsunan University, Hirakata, Osaka, Japan. kotani@pharm.setsunan.ac.jp
Isobe
M
M
Tochino
Y
Y
Takabatake
E
E
Chikaoka
Y
Y
Nomura
Y
Y
Tamura
M
M
D000818:Animals; D001591:Benzydamine / Q000031:analogs & derivatives / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
C048872:benzydamine N-oxide; D001591:Benzydamine; D010105:Oxygenases; C020066:dimethylaniline monooxygenase (N-oxide forming)