title: A new candidate region for the positional cloning of the XLP gene.
abstract: X-linked lymphoproliferative disease (XLP) is an inherited immunodeficiency characterised by selective susceptibility to Epstein-Barr virus and frequent association with malignant lymphomas chiefly located in the ileocecal region, liver, kidney and CNS. Taking advantage of a large bacterial clone contig, we obtained a genomic sequence of 197620 bp encompassing a deletion (XLP-D) of 116 kb in an XLP family, whose breakpoints were identified. The study of potential exons from this region in 40 unrelated XLP patients did not reveal any mutation. To define the critical region for XLP and investigate the role of the XLP-D deletion, detailed haplotypes in a region of approximately 20 cM were reconstructed in a total of 87 individuals from 7 families with recurrence of XLP. Two recombination events in a North American family and a new microdeletion (XLP-G) in an Italian family indicate that the XLP gene maps in the interval between DXS1001 and DXS8057, approximately 800 kb centromeric to the previously reported familial microdeletion XLP-D.
authors.affiliation: International XLP Consortium, Laboratorio di Genetica Molecolare, Istituto Gaslini, Genova, Quarto, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bolino
authors.forename: L
authors.initials: L
authors.lastname: Yin
authors.forename: M
authors.initials: M
authors.lastname: Seri
authors.forename: R
authors.initials: R
authors.lastname: Cusano
authors.forename: R
authors.initials: R
authors.lastname: Cinti
authors.forename: A
authors.initials: A
authors.lastname: Coffey
authors.forename: R
authors.initials: R
authors.lastname: Brooksbank
authors.forename: G
authors.initials: G
authors.lastname: Howell
authors.forename: D
authors.initials: D
authors.lastname: Bentley
authors.forename: J R
authors.initials: JR
authors.lastname: Davis
authors.forename: A
authors.initials: A
authors.lastname: Lanyi
authors.forename: D
authors.initials: D
authors.lastname: Huang
authors.forename: M
authors.initials: M
authors.lastname: Stark
authors.forename: M
authors.initials: M
authors.lastname: Creaven
authors.forename: L
authors.initials: L
authors.lastname: Bjørkhaug
authors.forename: F
authors.initials: F
authors.lastname: Heitzmann
authors.forename: J
authors.initials: J
authors.lastname: Lamartine
authors.forename: S
authors.initials: S
authors.lastname: Gaudi
authors.forename: B S
authors.initials: BS
authors.lastname: Sylla
authors.forename: G M
authors.initials: GM
authors.lastname: Lenoir
authors.forename: E
authors.initials: E
authors.lastname: Castagnola
authors.forename: R
authors.initials: R
authors.lastname: Giacchino
authors.forename: G
authors.initials: G
authors.lastname: Porta
authors.forename: B
authors.initials: B
authors.lastname: Franco
authors.forename: M
authors.initials: M
authors.lastname: Zollo
authors.forename: J
authors.initials: J
authors.lastname: Sumegi
authors.forename: G
authors.initials: G
authors.lastname: Romeo
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular; D017931:DNA Primers; D005260:Female; D017353:Gene Deletion; D008040:Genetic Linkage; D006239:Haplotypes; D006801:Humans; D008232:Lymphoproliferative Disorders / Q000235:genetics*; D008297:Male; D010375:Pedigree; D014960:X Chromosome
chemical_list: D017931:DNA Primers