Fasciola hepatica: partial characterization of circulating antigens.
Antigens present in sera from Fasciola hepatica-infected goats were partially characterized by means of polyacrylamide gel electrophoresis of immunoprecipitates obtained by counterimmunoelectrophoresis. Agar containing the 2 immunoprecipitin lines, agar containing no precipitin lines, a crude extract of F. hepatica, and normal rabbit IgG were assayed. The electrophoretic pattern of 1 precipitin line showed, apart from the light and heavy chains of IgG and other faint bands, 2 prominent polypeptides of about 70 and 85 kDa. The other precipitin line showed a similar pattern, but with the 70-kDa band absent.
Rodriguez-Osorio
M
M
Instituto de Parasitología y Biomedicina López-Neyra, Granada, Spain.
Rojas
J
J
Gómez-García
V
V
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D003378:Counterimmunoelectrophoresis / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005210:Fasciola hepatica / Q000276:immunology*; D005211:Fascioliasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D015511:Goat Diseases / Q000175:diagnosis* / Q000276:immunology; D006041:Goats; D007106:Immune Sera / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D011817:Rabbits
D000947:Antigens, Helminth; D007106:Immune Sera; D007074:Immunoglobulin G