title: Regional assignment and expression analysis of 29 expressed sequence tags mapped to chromosome 3.
abstract: Of 311 expressed sequenced tags (ESTs) mapped to single human chromosomes by analysis of a monochromosome somatic cell hybrid panel, 29 were localized to chromosome 3. Analysis of somatic cell hybrid lines containing different regions of chromosome 3 has enabled the regional assignment of these 29 ESTs to 13 of 23 intervals covering chromosome 3. Northern analysis of 25 of the EST clones has provided information on the pattern of expression of potential genes represented by these transcripts in 16 human tissue types. Nine of the clones hybridized solely to a transcript(s) in the testis, 12 hybridized to transcripts in testis and other tissues, and 4 hybridized with transcripts in testis and other tissues but in addition have testis-specific transcript sizes. These ESTs will provide useful markers throughout chromosome 3 for the development of physical and transcription maps. In addition, they provide candidate genes for disease loci mapping to the intervals defined by the chromosome 3 deletion panel.
authors.affiliation: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, England.
authors.forename: M H
authors.initials: MH
authors.lastname: Jones
authors.forename: K N
authors.initials: KN
authors.lastname: Tirosvoutis
authors.forename: C
authors.initials: C
authors.lastname: Bowgen
authors.forename: P
authors.initials: P
authors.lastname: Davey
authors.forename: S
authors.initials: S
authors.lastname: Moore
authors.forename: S
authors.initials: S
authors.lastname: Naylor
authors.forename: N A
authors.initials: NA
authors.lastname: Affara
mesh_terms: D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D004247:DNA / Q000235:genetics; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D006822:Hybrid Cells; D008297:Male; D013737:Testis / Q000378:metabolism
chemical_list: D004247:DNA