Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum.
Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.
Thomas
C J
CJ
NERC Institute of Virology and Environmental Microbiology, Oxford OX1 3SR, United Kingdom.
Brown
H L
HL
Hawes
C R
CR
Lee
B Y
BY
Min
M K
MK
King
L A
LA
Possee
R D
RD
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002688:Chitinases / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology* / Q000821:virology*; D007814:Larva / Q000201:enzymology / Q000821:virology; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D008969:Molecular Sequence Data; D009036:Moths / Q000201:enzymology / Q000254:growth & development / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D021382:Protein Sorting Signals / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera
D021382:Protein Sorting Signals; D002688:Chitinases