title: Daily cycle of bHLH-PAS proteins, Ah receptor and Arnt, in multiple tissues of female Sprague-Dawley rats.
abstract: The aryl hydrocarbon receptor (AhR) shares a common PAS domain with a number of genes that exhibit a pronounced circadian rhythm. Therefore, this study examined the daily cycle of AhR and AhR nuclear translocator (Arnt) protein expression in multiple tissues of female Sprague-Dawley rats. Rats were euthanized at 4, 7, and 11 am and 4, 7, and 11 pm after which whole tissue homogenates were made from multiple tissues. Western blot analysis showed that the daily cycle of relative AhR protein expression exhibits a similar oscillation pattern in the liver, lungs, and thymus. The daily cycle of relative Arnt protein expression exhibits a similar oscillation pattern in the liver and lungs. The apparent daily cycle of AhR and Arnt protein expression in multiple tissues was not observed within the spleen. This preliminary report is the first study to suggest that the PAS proteins, AhR and Arnt, exhibit a daily oscillation pattern within multiple target tissues which may give insight into the tissue-specific toxic and biochemical responses mediated through this dimerization pair, as well as the physiological function of these proteins.
authors.affiliation: U.S. Environmental Protection Agency, National Health & Environmental Effects Research Laboratory, Research Triangle Park, North Carolina, 27711, USA.
authors.forename: V M
authors.initials: VM
authors.lastname: Richardson
authors.forename: M J
authors.initials: MJ
authors.lastname: Santostefano
authors.forename: L S
authors.initials: LS
authors.lastname: Birnbaum
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator; D002940:Circadian Rhythm; D004268:DNA-Binding Proteins; D005260:Female; D005786:Gene Expression Regulation; D018257:Helix-Loop-Helix Motifs; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D009991:Oscillometry; D051381:Rats; D017207:Rats, Sprague-Dawley; D018336:Receptors, Aryl Hydrocarbon / Q000235:genetics*; D013950:Thymus Gland / Q000378:metabolism; D013995:Time; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C497398:ARNT protein, rat; D004268:DNA-Binding Proteins; D018336:Receptors, Aryl Hydrocarbon; D014157:Transcription Factors; D051784:Aryl Hydrocarbon Receptor Nuclear Translocator