title: Pim-1 kinase and p100 cooperate to enhance c-Myb activity.
abstract: The pim-1 oncogene is regulated by hematopoietic cytokine receptors, encodes a serine/threonine protein kinase, and cooperates with c-myc in lymphoid cell transformation. Using a yeast two-hybrid screen, we found that Pim-1 protein binds to p100, a transcriptional coactivator that interacts with the c-Myb transcription factor. Pim-1 phosphorylated p100 in vitro, formed a stable complex with p100 in animal cells, and functioned downstream of Ras to stimulate c-Myb transcriptional activity in a p100-dependent manner. Thus, Pim-1 and p100 appear to be components of a novel signal transduction pathway affecting c-Myb activity, linking all three to the cytokine-regulated control of hematopoietic cell growth, differentiation, and apoptosis.
authors.affiliation: Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208-3500, USA.
authors.forename: J D
authors.initials: JD
authors.lastname: Leverson
authors.forename: P J
authors.initials: PJ
authors.lastname: Koskinen
authors.forename: F C
authors.initials: FC
authors.lastname: Orrico
authors.forename: E M
authors.initials: EM
authors.lastname: Rainio
authors.forename: K J
authors.initials: KJ
authors.lastname: Jalkanen
authors.forename: A B
authors.initials: AB
authors.lastname: Dash
authors.forename: R N
authors.initials: RN
authors.lastname: Eisenman
authors.forename: S A
authors.initials: SA
authors.lastname: Ness
mesh_terms: D000123:Acetyltransferases; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004720:Endonucleases; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D020598:Proto-Oncogene Proteins c-myb; D051573:Proto-Oncogene Proteins c-pim-1; D015398:Signal Transduction / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D018631:ras Proteins / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D011506:Proteins; D011518:Proto-Oncogene Proteins; D020598:Proto-Oncogene Proteins c-myb; D015534:Trans-Activators; D000123:Acetyltransferases; C077509:mim-1 protein, Gallus gallus; C496636:Pim1 protein, mouse; D017346:Protein Serine-Threonine Kinases; D051573:Proto-Oncogene Proteins c-pim-1; D004720:Endonucleases; C490640:Snd1 protein, mouse; D018631:ras Proteins