title: Potential use of repeated fluorescence in situ hybridization in the same human blastomeres for preimplantation genetic diagnosis.
abstract: OBJECTIVE
  To assess the feasibility of repeated fluorescence in situ hybridization (FISH) procedures in the same nucleus of a human blastomere.
  
  
  METHODS
  Three consecutive FISH procedures were performed in the same human blastomere by using direct label fluorescence CEP and WCP probes (Vysis).
  
  
  METHODS
  Hospital-based private IVF program.
  
  
  METHODS
  Twenty-eight infertile couples who underwent conventional IVF in our center.
  
  
  METHODS
  Embryos from oocytes with three pronuclei after in vitro insemination were used in this study.
  
  
  METHODS
  The rates of nuclear loss, present signals, and absent signal were examined.
  
  
  RESULTS
  In group 1, the rates of presence of signals were 94% after the first FISH, 92% after the second FISH, and 88% after the third FISH. In group 2, the rates of presence of signals were 96% after the first FISH, 93% after the second FISH, and 87% after the third FISH. There was no statistically significant difference in the rates of nuclear loss, present signals, and absent signal between three consecutive FISH procedures and between CEP and WCP probes.
  
  
  CONCLUSIONS
  Six or more chromosomes of a single blastomere may be examined with use of this repeated FISH procedure, which may be important for preimplantation genetic diagnosis.
authors.affiliation: The Greater Baltimore Medical Center Fertility Center, Maryland 21204, USA. jliu@gbmc.org
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: Y L
authors.initials: YL
authors.lastname: Tsai
authors.forename: X Z
authors.initials: XZ
authors.lastname: Zheng
authors.forename: T A
authors.initials: TA
authors.lastname: Baramki
authors.forename: R A
authors.initials: RA
authors.lastname: Yazigi
authors.forename: E
authors.initials: E
authors.lastname: Katz
mesh_terms: D001757:Blastomeres; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*