title: High implantation and pregnancy rates with transfer of human blastocysts developed in preimplantation stage one and blastocyst media.
abstract: OBJECTIVE
  To evaluate the proficiency of preimplantation stage one (P1) and blastocyst media in supporting human blastocyst development and to document implantation and clinical pregnancy rates from the transfer of the normally developed blastocysts.
  
  
  METHODS
  Retrospective clinical study.
  
  
  METHODS
  Private IVF unit of a university-affiliated center.
  
  
  METHODS
  Twenty-eight women aged 33.7 +/- 2.9 years who underwent IVF treatment for infertility.
  
  
  METHODS
  Bipronucleate oocytes obtained from IVF and intracytoplasmic sperm injection were cultured in vitro with P1 and blastocyst media for 96-120 hours. One to three embryos were transferred (2.1 +/- 0.2 for the patients who became pregnant and 1.5 +/- 0.3 for those who did not become pregnant).
  
  
  METHODS
  Total number and percentage of developed blastocysts, frequency of blastocysts of grades A and B, and implantation and pregnancy rates.
  
  
  RESULTS
  From 431 oocytes retrieved, 269 bipronucleate oocytes were cultured, producing 81 blastocysts that resulted in the transfer of 54 embryos in 27 procedures. Blastocysts developed in 39.7% +/- 5.5% of the pregnant group and 30.2% +/- 4.5% of the nonpregnant group. From 15 (15/27 = 55.6%) clinical pregnancies, 18 (18/54 = 33.3%) gestational sacs were visualized. The rate of implantation in the pregnant group was 58.1% (18/31).
  
  
  CONCLUSIONS
  These results provide evidence for the benefits of extending human embryo culture with P1 and blastocyst media for all normally fertilized embryos in vitro.
authors.affiliation: Centro de Medicina Reprodutiva Huntington São Paulo, SP, Brazil. bebefiv@uol.com.br
authors.forename: E L
authors.initials: EL
authors.lastname: Alves da Motta
authors.forename: J R
authors.initials: JR
authors.lastname: Alegretti
authors.forename: E C
authors.initials: EC
authors.lastname: Baracat
authors.forename: D
authors.initials: D
authors.lastname: Olive
authors.forename: P C
authors.initials: PC
authors.lastname: Serafini
mesh_terms: D000328:Adult; D017677:Age Distribution; D001755:Blastocyst / Q000502:physiology; D003470:Culture Media; D010064:Embryo Implantation; D004624:Embryo Transfer; D047108:Embryonic Development; D005314:Embryonic and Fetal Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D012189:Retrospective Studies
chemical_list: D003470:Culture Media