Delayed progression of murine AIDS in C57BL/6 mice pre-immunized with a highly antigenic 10-mer peptide encoded by the murine AIDS defective virus gag p12 gene.
C57BL/6 (B6) mice were immunized with a highly antigenic 10-mer peptide (P12-10), which is encoded by the murine AIDS (MAIDS) defective virus gag p12 gene, emulsified in incomplete Freund's adjuvant (ICFA). One week later, the mice were inoculated with the MAIDS virus to see if the immunization affects progression of MAIDS. It was demonstrated that the immunization significantly delayed progression of MAIDS, although it failed to induce appreciable cytotoxic T lymphocyte (CTL) responses against the P12-10 antigen. In contrast, immunization of B6 mice with the P12-10 coupled with liposome induced substantial CTL responses but failed to protect the mice against MAIDS development. This segregation between CTL activity and in vivo protection efficacy might be worth considering when we exploit vaccines for augmenting cellular immunity mediated by CD8+ T cells.
Mizuochi
T
T
Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Tokyo, Japan. miz@nih.go.jp
Horino
A
A
Uchida
T
T
D000818:Animals; D003673:Defective Viruses / Q000737:chemistry / Q000276:immunology / Q000472:pathogenicity; D018450:Disease Progression; D005620:Freund's Adjuvant / Q000276:immunology; D015683:Gene Products, gag / Q000235:genetics / Q000276:immunology* / Q000494:pharmacology; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D009052:Leukemia Virus, Murine / Q000737:chemistry / Q000276:immunology; D008055:Lipids; D008081:Liposomes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D016183:Murine Acquired Immunodeficiency Syndrome / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
D015683:Gene Products, gag; D015395:Histocompatibility Antigens Class I; D008055:Lipids; D008081:Liposomes; C114843:incomplete Freund's adjuvant; D005620:Freund's Adjuvant