title: Transport of intact porphyrin by HpuAB, the hemoglobin-haptoglobin utilization system of Neisseria meningitidis.
abstract: The meningococcal hemA gene was cloned and used to construct a porphyrin biosynthesis mutant. An analysis of the hemA mutant indicated that meningococci can transport intact porphyrin from heme (Hm), hemoglobin (Hb), and Hb-haptoglobin (Hp). By constructing a HemA- HpuAB- double mutant, we demonstrated that HpuAB is required for the transport of porphyrin from Hb and Hb-Hp.
authors.affiliation: Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73103, USA. llewis@rex.uokhsc.edu
authors.forename: L A
authors.initials: LA
authors.lastname: Lewis
authors.forename: M H
authors.initials: MH
authors.lastname: Sung
authors.forename: M
authors.initials: M
authors.lastname: Gipson
authors.forename: K
authors.initials: K
authors.lastname: Hartman
authors.forename: D W
authors.initials: DW
authors.lastname: Dyer
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D004269:DNA, Bacterial; D006242:Haptoglobins / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism*; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009345:Neisseria meningitidis / Q000235:genetics / Q000378:metabolism*; D011166:Porphyrins / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D006242:Haptoglobins; D006454:Hemoglobins; C105625:HpuB protein, Neisseria; D008074:Lipoproteins; D011166:Porphyrins; D000445:Aldehyde Oxidoreductases; C067282:glutamyl tRNA reductase