title: The role of quaternary interactions on the stability and activity of ascorbate peroxidase.
abstract: Point mutations at the dimer interface of the homodimeric enzyme ascorbate peroxidase (APx) were constructed to assess the role of quaternary interactions in the stability and activity of APx. Analysis of the APx crystal structure shows that Glu112 forms a salt bridge with Lys20 and Arg24 of the opposing subunit near the axis of dyad symmetry between the subunits. Two point mutants, E112A and E112K, were made to determine the effects of a neutral (alanine) and repulsive (lysine) mutation on dimerization, stability, and activity. Gel filtration analysis indicated that the ratio of the monomer to dimer increased as the dimer interface interactions went from attractive to repulsive. Differential scanning calorimetry (DSC) data exhibited a decrease in both the transition temperature (Tm) and enthalpy of unfolding (deltaHc) with Tm = 58.3 +/- 0.5 degrees C, 56.0 +/- 0.8 degrees C, and 53.0 +/- 0.9 degrees C and deltaHc = 245 +/- 29 kcal/mol, 199 +/- 38 kcal/mol, and 170 +/- 25 kcal/mol for wild-type APx, E112A, and E112K, respectively. Similar changes were observed based on thermal melting curves obtained by absorption spectroscopy. No change in enzyme activity was found for the E112A mutant, and only a 25% drop in activity was observed for the E112K mutant which demonstrates that the non-Michaelis Menten kinetics of APx is not due to the APx oligomeric structure. The cryogenic crystal structures of the wild-type and mutant proteins show that mutation induced changes are limited to the dimer interface including an alteration in solvent structure.
authors.affiliation: University of California at Irvine, Department of Molecular Biology and Biochemistry, 92697-3900, USA.
authors.forename: D
authors.initials: D
authors.lastname: Mandelman
authors.forename: F P
authors.initials: FP
authors.lastname: Schwarz
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: T L
authors.initials: TL
authors.lastname: Poulos
mesh_terms: D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D018360:Crystallography, X-Ray; D019281:Dimerization; D004795:Enzyme Stability; D006860:Hydrogen Bonding; D007700:Kinetics; D008958:Models, Molecular; D008970:Molecular Weight; D010544:Peroxidases / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry; D017354:Point Mutation / Q000235:genetics; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013816:Thermodynamics; D014867:Water / Q000737:chemistry
chemical_list: D010940:Plant Proteins; D014867:Water; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D001205:Ascorbic Acid