Inhibition of succinic semialdehyde dehydrogenase by N-formylglycine.
N-formylglycine was developed as a dead-end inhibitor of the succinic semialdehyde dehydrogenase reaction. At 4 mM, it inhibited Aspergillus niger succinic semialdehyde dehydrogenase by 40%. N-formylglycine is a reversible, complete inhibitor; the inhibition is competitive with succinic semialdehyde and uncompetitive with respect to NAD+ and the Ki values are 4.9 and 10.4 mM respectively. Potato succinic semialdehyde dehydrogenase is also inhibited by N-formylglycine to a similar extent, the nature of the inhibition being identical to that observed with the A. niger enzyme.
Kumar
S
S
Biotechnology Centre, Indian Institute of Technology-Bombay, Powai, Mumbai, India.
Punekar
N S
NS
D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors*; D001234:Aspergillus niger / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005998:Glycine / Q000031:analogs & derivatives* / Q000494:pharmacology; D011198:Solanum tuberosum / Q000201:enzymology; D050644:Succinate-Semialdehyde Dehydrogenase
D004791:Enzyme Inhibitors; C032736:N-formylglycine; D000445:Aldehyde Oxidoreductases; D050644:Succinate-Semialdehyde Dehydrogenase; D005998:Glycine