Primer tRNA(Trp) of RSV-transformed or RAV-1-infected cells up-regulates the antiribosomal activity of gelonin.
Some ribosome-inactivating proteins (RIPs) with RNA-N-glycosidase activity on 28S rRNA require, for maximal inactivation of ribosomes, the presence of tRNA. tRNA(Trp) specifically up-regulates gelonin, the RIP from Gelonium multiflorum. The same tRNA is the primer of the reverse transcriptase of Rous sarcoma virus (RSV) and of its mutant (RAV-1) which lacks the src gene. Here we demonstrate that gelonin is more active in inhibiting endogenous protein synthesis by lysates of RSV-transformed or RAV-1-infected cells and that such increase in activity correlates with the increased amount of primer tRNA(Trp) in the cells.
Brigotti
M
M
Dipartimento di Patologia sperimentale, Università di Bologna, Italy.
Petronini
P G
PG
Borghetti
A F
AF
Guidotti
G G
GG
Sperti
S
S
Montanaro
L
L
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000502:physiology; D002460:Cell Line; D002461:Cell Line, Transformed; D002642:Chick Embryo; D010940:Plant Proteins / Q000494:pharmacology*; D012313:RNA / Q000494:pharmacology*; D012364:RNA, Transfer, Trp / Q000235:genetics*; D054789:Ribosome Inactivating Proteins, Type 1; D012270:Ribosomes / Q000187:drug effects*; D015854:Up-Regulation / Q000187:drug effects*
D010940:Plant Proteins; C034477:RNA primers; D012364:RNA, Transfer, Trp; D054789:Ribosome Inactivating Proteins, Type 1; D012313:RNA; C025674:GEL protein, Gelonium multiflorum