Proof of hepatitis A virus negative-sense RNA by RNA/DNA-hybrid detection: a method for specific detection of both viral negative- and positive-strand RNA species.
The detection of hepatitis A virus (HAV) negative-strand RNA, which is synthesized during replication of the positive-strand RNA genome, proved to be difficult. We developed a method for the specific detection of HAV negative-strand RNA by RNA-DNA hybridization and luminescence detection using an anti-RNA:DNA hybrid antibody. This method, which is also applicable for the specific detection of positive-strand RNA, offers a simple, yet relatively rapid and certain means of detecting low amounts of RNA such as HAV negative-strand RNA. By using appropriate hybridization DNA probes, the method should be applicable for the detection of single-stranded RNA species of different viruses in general.
Schmitz
G
G
Department of Virology, University of Bremen, Leobener Strasse/UFT, D-28359 Bremen, Germany.
Dotzauer
A
A
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D015342:DNA Probes / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D006507:Hepatovirus / Q000235:genetics*; D008253:Macaca mulatta; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012367:RNA, Viral / Q000032:analysis* / Q000235:genetics*
D015342:DNA Probes; D004279:DNA, Viral; D012367:RNA, Viral