Divercin V41, a new bacteriocin with two disulphide bonds produced by Carnobacterium divergens V41: primary structure and genomic organization.
Divercin V41 is a new bacteriocin produced by Carnobacterium divergens V41, a lactic acid bacterium isolated from fish viscera. The amino acid sequence of divercin V41 showed high homologies with pediocin PA-1 and enterocin A. Two disulphide bonds were present in the hydrophilic N-terminal domain and in the highly variable hydrophobic C-terminal domain, respectively. A DNA probe designed from the N-terminal sequence of the purified peptide was used to locate the structural gene of divercin V41. A 6 kb chromosomal fragment containing the divercin V41 structural gene (dvnA) was cloned and sequenced. The results indicate that divercin V41 is synthesized as a pre-bacteriocin of 66 amino acids. The 23-residue N-terminal extension is cleaved off to yield the mature 43-amino-acid divercin V41. In addition, the fragment encodes putative proteins commonly found within bacteriocin operons, including an ATP-dependent transporter, two immunity-like proteins and the two components of a lantibiotic-type signal-transducing system. The genetic organization of the fragment suggested important gene rearrangements.
Metivier
Anita
A
lnstitut de Biologie MolCculaire et d`lngCnierie GCnCtiqueCNRS-ESA 6031, Universite de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers CedexFrance.|ENITIAA, Laboratoire de Microbiologie44072 Nantes CedexFrance.
Pilet
Marie-France
MF
ENITIAA, Laboratoire de Microbiologie44072 Nantes CedexFrance.
Dousset
Xavier
X
ENITIAA, Laboratoire de Microbiologie44072 Nantes CedexFrance.
Sorokine
Odile
O
CNRS, Laboratoire de SpectromCtrie de Masse BioorganiqueURA31 Universite Louis Pasteur, 67008 StrasbourgFrance.
Anglade
Patricia
P
INRA, Domaine de Vilvert78352 Jouy en Josas cedexFrance.
Zagorec
Monique
M
INRA, Domaine de Vilvert78352 Jouy en Josas cedexFrance.
Piard
Jean-Christophe
JC
INRA, Domaine de Vilvert78352 Jouy en Josas cedexFrance.
Marlon
Didier
D
INRA, Unite de Biochimie et Technologie des ProtCines44316 Nantes cedexFrance.
Cenatiempo
Yves
Y
lnstitut de Biologie MolCculaire et d`lngCnierie GCnCtiqueCNRS-ESA 6031, Universite de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers CedexFrance.
Fremaux
Christophe
C
T6exel, groupe RhBnePoulencZA de Buxieres BP 10,86220 DangC Saint-RomainFrance.|lnstitut de Biologie MolCculaire et d`lngCnierie GCnCtiqueCNRS-ESA 6031, Universite de Poitiers, 40 avenue du Recteur Pineau, 86022 Poitiers CedexFrance.
D000595:Amino Acid Sequence; D001224:Aspartic Acid / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D001430:Bacteriocins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003545:Cysteine / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry* / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D015698:Genomic Library; D007777:Lactobacillaceae / Q000378:metabolism*; D013058:Mass Spectrometry; D008666:Metalloendopeptidases; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D010455:Peptides / Q000737:chemistry; D016133:Polymerase Chain Reaction; D011725:Pyridines / Q000378:metabolism; D016415:Sequence Alignment; D017421:Sequence Analysis
D001426:Bacterial Proteins; D001430:Bacteriocins; D004220:Disulfides; D010455:Peptides; D011725:Pyridines; C116372:divercin V41; D001224:Aspartic Acid; D010450:Endopeptidases; D008666:Metalloendopeptidases; C058921:endoproteinase Asp-N; C029351:4-vinylpyridine; D003545:Cysteine