Simple and broadly applicable sample preparation by use of magnetic glass particles.
A key issue in nucleic acid-based assays related to infectious disease testing and genetic testing is the sample preparation. This has to be convenient, efficient and suitable for automation. We describe a new method based on the use of glass magnetic particles. The steps involved are the lysis of bacterial cells and viral particles by chaotropic salts, binding of released nucleic acids to the magnetic glass particles, the removal of inhibitors and the release of purified nucleic acids ready for PCR amplification. The method fulfills most of the requirements for sample preparation such as sensitivity, universality and suitability for automation.
Bartl
K
K
Roche Diagnostics Boehringer Mannheim GmbH, Tutzing, Germany. Knut.Bartl@Roche.com
Wenzig
P
P
Kleiber
J
J
D004247:DNA / Q000652:urine*; D005898:Glass; D006801:Humans; D008280:Magnetics; D012313:RNA / Q000652:urine*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
D012313:RNA; D004247:DNA