title: Anti-beta2-glycoprotein I antibodies.
abstract: The relationship between presence of anti-beta2-glycoprotein I autoantibodies (abeta2-GPI) and history of thrombosis is now widely known. However, differences in the methodology of abeta2-GPI detection have made the comparison of data from different laboratories extremely difficult. We discuss the significance of abeta2-GPI of the IgG, IgM and IgA isotypes, and our approach to developing an easier and more reproducible method for the detection of this autoantibody. In addition, we present data that shows that commercially available enzyme immunoassay plates differ regarding detectability of abeta2-GPI. Since the clinical significance of this heterogeneity is presently unclear, the set-up of the detection systems and interpretation of data need great care.
authors.affiliation: Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Tsutsumi
authors.forename: K
authors.initials: K
authors.lastname: Ichikawa
authors.forename: E
authors.initials: E
authors.lastname: Matsuura
authors.forename: T
authors.initials: T
authors.lastname: Koike
mesh_terms: D000818:Animals; D017153:Antibodies, Anticardiolipin / Q000097:blood / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000276:immunology; D000937:Antigen-Antibody Reactions; D016736:Antiphospholipid Syndrome / Q000175:diagnosis / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis / Q000276:immunology*; D000939:Epitopes / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D046911:Macromolecular Substances; D051379:Mice; D008815:Mice, Inbred Strains; D011933:Reagent Kits, Diagnostic; D012015:Reference Standards; D053482:beta 2-Glycoprotein I
chemical_list: D017153:Antibodies, Anticardiolipin; D000911:Antibodies, Monoclonal; D001324:Autoantigens; D000939:Epitopes; D006023:Glycoproteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D046911:Macromolecular Substances; D011933:Reagent Kits, Diagnostic; D053482:beta 2-Glycoprotein I