Position effect variegations and brain-specific silencing in transgenic mice overexpressing human acetylcholinesterase variants.
Position effect variegations as well as brain-specific silencing were observed in novel transgenic mouse pedigrees expressing human acetylcholinesterase (AChE) variants. Muscle AChE activities varied between 1.6- and 350-fold of control in these lines, one carrying insertion-inactivated InE6-AChE and two with 'readthrough' I4/E5 AChE, all under control of the ubiquitous CMV promoter. In contrast, brain AChE levels remained within a range of 1.5-fold over control, suggesting an upper limit of brain AChE which is compatible with life.
Sternfeld
M
M
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Patrick
J D
JD
Soreq
H
H
D000110:Acetylcholinesterase / Q000235:genetics*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D014644:Genetic Variation; D016678:Genome; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D009132:Muscles / Q000201:enzymology; D009473:Neuronal Plasticity / Q000502:physiology; D009928:Organ Specificity; D010375:Pedigree; D013315:Stress, Psychological / Q000378:metabolism; D014981:Xenopus
D000110:Acetylcholinesterase