Effects of beta-lactoglobulin on the tight-junctional stability of Caco-2-SF monolayer.
The mechanisms for tight-junction (TJ) stabilization by beta-lactoglobulin (beta-Lg) were studied. Treatment of Caco-2-SF cells with inhibitors for some enzymes in the intracellular signal transduction pathways and a cytoskeleton-disturbing agent (cytochalasin D) reduced the TJ-stabilizing activity of beta-Lg. So beta-Lg is suggested to modulate the cytoskeletal structure through the activation of phospholipase C and protein kinase C, resulting in the TJ stabilization.
Hashimoto
K
K
Department of Bioproductive Science, Utsunomiya University, Japan. keih@cc.utsunomiya-u.ac.jp
Nakayama
T
T
Shimizu
M
M
D000818:Animals; D018938:Caco-2 Cells; D002417:Cattle; D006801:Humans; D007782:Lactoglobulins / Q000378:metabolism*; D019108:Tight Junctions / Q000502:physiology*
D007782:Lactoglobulins