Simple method for determination of hydrochlorothiazide in human urine by high performance liquid chromatography utilizing narrowbore chromatography.
A simple high performance liquid chromatographic (HPLC) method utilizing narrowbore chromatography was developed for the determination of hydrochlorothiazide in human urine. A mobile phase of 0.1% aqueous acetic acid--acetonitrile (93:7, v/v) pH 3 was used with a C18 analytical column and ultraviolet detection (UV). The method demonstrated linearity from 2 to 50 micrograms ml-1 using 50 microliters of urine with a detection limit of 1 microgram ml-1. The method was utilized in a study evaluating if racial differences are present in the pharmacokinetic and pharmacodynamic effects of hydrochlorothiazide.
Farthing
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Division of Clinical Pharmacology and Hypertension, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298-0160, USA.
Fakhry
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I
Ripley
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EB
Sica
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D
D044383:Black People; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004232:Diuretics; D005260:Female; D006801:Humans; D006852:Hydrochlorothiazide / Q000493:pharmacokinetics / Q000652:urine*; D006973:Hypertension / Q000208:ethnology / Q000652:urine; D008297:Male; D012680:Sensitivity and Specificity; D049993:Sodium Chloride Symporter Inhibitors / Q000493:pharmacokinetics / Q000652:urine*; D044465:White People
D004232:Diuretics; D049993:Sodium Chloride Symporter Inhibitors; D006852:Hydrochlorothiazide