[Follicular conjunctivitis caused by an adenovirus. The characterization of its strains by restriction endonucleases].
A study of conjunctivitis cases with a possible viral cause was carried out in Havana City during June and August, 1993. Thirty seven cases from "Ramón Pando Ferrer" Ophthalmological Hospital were studied, of whom 13 isolations (35.1%) in cell culture of Hela cells were obtained. Isolations showed a cytopathic effect characteristic of Adenovirus. A direct immunofluorescence with conjugated serum of rabbit specific to Adenovirus was performed and results were positive in 100% of cases. A DNA restriction analysis of each isolated was performed for their classification. Restriction endonucleases SmaI and Hind III were used and 2 isolates were classified as Adenovirus type 3, and 6 as Adenovirus type 7b, 4 out of 13 isolates were not classified because of the degradation of the genetic material.
Savón
C
C
Instituto de Medicina Tropical Pedro Kourí.
Muné
M
M
Goyenechea
A
A
Valdivia
A
A
Soto
Y
Y
Pérez
L
L
D000258:Adenovirus Infections, Human / Q000821:virology*; D000260:Adenoviruses, Human / Q000145:classification / Q000302:isolation & purification*; D003236:Conjunctivitis, Viral / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D019085:Fluorescent Antibody Technique, Direct; D006367:HeLa Cells; D006801:Humans; D015183:Restriction Mapping
D004279:DNA, Viral