title: Protection by alpha-tocopherol but not ascorbic acid from hydrogen peroxide induced cell death in normal human breast epithelial cells in culture.
abstract: Alpha-tocopherol and ascorbic acid have been suggested to play a role in breast cancer prevention due to their antioxidative capacity. Increased exposure to endogenous and exogenous sex steroids is a known risk factor for breast cancer. We have studied the effects of alpha-tocopherol and ascorbic acid on hydrogen peroxide induced cell death in sex hormone treated normal breast epithelial cells in culture. We found that alpha-tocopherol but not ascorbic acid alone protected the cells. The effect of alpha-tocopherol increased when ascorbic acid was added to the cultures. The hydrogen peroxide degradation rate decreased in cultures treated with alpha-tocopherol alone and in combination with ascorbic acid compared to cells grown in medium or with ascorbic acid only. Oestradiol and progesterone treatment did not influence the results. Possible beneficial effects of combining various antioxidants, endogenous as well as exogenous, on human breast tissue need to be investigated further both in vivo and in vitro.
authors.affiliation: Department of Obstetrics and Gynaecology, Faculty of Health Sciences, University Hospital, Linköping, Sweden. lotda@ihm.liu.se
authors.forename: C
authors.initials: C
authors.lastname: Dabrosin
authors.forename: K
authors.initials: K
authors.lastname: Ollinger
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology*; D001940:Breast / Q000166:cytology* / Q000187:drug effects; D016923:Cell Death / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011374:Progesterone / Q000494:pharmacology; D014810:Vitamin E / Q000032:analysis / Q000494:pharmacology*
chemical_list: D014810:Vitamin E; D011374:Progesterone; D004958:Estradiol; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D001205:Ascorbic Acid