Identification of a mutation (A1879G) of transferrin from cDNA prepared from peripheral blood cells.
We have prepared a transferrin (TF) cDNA from total RNA of peripheral blood cells of an individual with a TF B variant (TF Bv) phenotype to analyze the molecular basis for the TF Bv. The TF B variant allele (TF Bv) was found to contain an A to G transition at nucleotide 1879 in the coding region that may result in substitution of glutamic acid for lysine at codon 627 located in exon 16. In addition, a transient expression study by transfection of each plasmid containing TF C1, TF C2 or TF Bv cDNA into monkey kidney (COS7) cells indicated that the products from specific TF alleles were able to be identified respective phenotypes by isoelectrofocusing electrophoresis and immunoblotting analyses. Based on this nucleotide substitution, we also established a PCR-RFLP method to detect the TF Bv allele from genomic DNA. Thus, peripheral blood cells were useful as an alternative source for preparation of cDNA from human liver for the molecular analysis of TF polymorphism.
Pang
H
H
Department of Forensic Medicine, Kurume University School of Medicine, Japan.
Koda
Y
Y
Soejima
M
M
Kimura
H
H
D000818:Animals; D001483:Base Sequence; D001773:Blood Cells / Q000378:metabolism; D019556:COS Cells; D017931:DNA Primers; D018076:DNA, Complementary; D006579:Heterozygote; D006801:Humans; D009154:Mutation; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D014168:Transferrin / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D014168:Transferrin