title: Deep uterine insemination of cattle: a fruitful way forward with smaller numbers of spermatozoa.
abstract: After describing the site of fertilisation and that of the functional sperm reservoir in the female tract, proposals are made concerning a modified site of sperm deposition in cattle. By means of a deep pre-ovulatory insemination into the ipsilateral uterine horn, the chances should be raised of establishing viable spermatozoa in the isthmus where they would undergo a form of physiological encapsulation and storage. Release and activation of such spermatozoa would be prompted by imminent ovulation. Potential advantages of this approach include those of raising the overall fertility of genetically valuable bulls whose non-return rates are sub-optimal; reducing the number of spermatozoa in each insemination dose; using effectively the limited numbers of sex-selected sperm cells (X and Y chromosome bearing spermatozoa) currently available from flow cytometry. Putative disadvantages might include rectal palpation of the ovaries to locate the pre-ovulatory follicle; perforation of the uterine wall by the deep insemination catheter; risk of polyspermic fertilisation; and the inappropriateness of the technique for non-clinically qualified inseminators. Each of these reservations is responded to in a rational manner. Given a change of attitude, a modified technique of insemination would be feasible under commercial conditions and might give a welcome boost to a sagging artificial insemination industry.
authors.affiliation: Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. TG@kvl.dk
authors.forename: R H
authors.initials: RH
authors.lastname: Hunter
authors.forename: T
authors.initials: T
authors.lastname: Greve
mesh_terms: D000818:Animals; D002417:Cattle; D005260:Female; D005836:Genitalia, Female / Q000033:anatomy & histology; D007315:Insemination, Artificial / Q000379:methods / Q000662:veterinary*; D008297:Male