Capillary zone electrophoretic determination of the four vitamin C esters L-ascorbyl-2-phosphate, L-ascorbyl-2-sulfate, L-ascorbyl-2-diphosphate and L-ascorbyl-2-triphosphate in fish feed, plasma and tissue.
A new and fast method for the determination of L-ascorbyl-2-phosphate, the 2-diphosphate, 2-triphosphate and the 2-sulfate ester in feed, fish plasma and tissue samples by capillary zone electrophoresis is described. The substances were extracted with a 200 mM potassium phosphate buffer pH 4.0. Separation was achieved using a 50 microm uncoated fused-silica bubble capillary of 40 cm length to the detector, which was set at 254 nm, and a 80 mM tricine buffer at pH 9.2. Repeatability checked on a trout feed sample revealed a migration time variation of 1.2% (n=24) and an area calculation variation of 2.8% (n=24). Detector response for L-ascorbyl-2-phosphate was linear up to at least 100 microg ml(-1). After administration of a high dose of L-ascorbyl-2-phosphate to rainbow trout, the compound was found in the stomach and intestine but not in plasma, proving a fast conversion to vitamin C.
Pauli
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NM
F. Hoffmann-La Roche Ltd., Vitamins and Fine Chemicals, Basle, Switzerland.
Schuep
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D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D004952:Esters; D017686:Oncorhynchus mykiss / Q000097:blood; D015203:Reproducibility of Results
D004952:Esters; D001205:Ascorbic Acid