Oxidised LDL (OxLDL) induces production of platelet derived growth factor AA (PDGF AA) from aortic smooth muscle cells.
OBJECTIVE
Elevated concentrations of oxidised low density lipoproteins (OxLDL) are associated with accelerated atherogenesis. The aim of our study was to determine the effect of OxLDL on the proliferation rate and platelet derived growth factor (PDGF) AA production on aortic smooth muscle cells. High density lipoproteins (HDL), which are known to have a protective effect against atherosclerosis, were used as control.


METHODS
Bovine aortic smooth muscle cells were grown in presence of increased concentrations of OxLDL and HDL and in presence of control medium culture (DMEM). Proliferation rate was assessed by 3H-thymidine uptake. PDGF AA production was determined by ELISA and Western Blot Analysis.


RESULTS
OxLDL increased the proliferation rate of aortic smooth muscle cells as compared to DMEM and HDL (p < 0.001). The mitogenic activity of OxLDL on smooth muscle cells was reduced adding anti-PDGF AA antibodies (p < 0.001). PDGF AA production by aortic smooth muscle cells was increased after exposure to OxLDL as compared to DMEM (p < 0.001). HDL significantly reduced the production of PDGF AA by aortic smooth muscle cells (p < 0.001).


CONCLUSIONS
Part of the atherogenic effect of OxLDL is mediated through the autocrine production of PDGF AA from aortic smooth muscle cells.
Cucina
A
A
First Department of Surgery, University of Rome La Sapienza, Italy.
Pagliei
S
S
Borrelli
V
V
Corvino
V
V
Stipa
F
F
Cavallaro
A
A
Sterpetti
A V
AV
D000818:Animals; D001013:Aorta, Thoracic; D001161:Arteriosclerosis / Q000209:etiology; D015153:Blotting, Western; D002417:Cattle; D002455:Cell Division; D008075:Lipoproteins, HDL / Q000494:pharmacology; D008077:Lipoproteins, LDL / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010982:Platelet-Derived Growth Factor / Q000096:biosynthesis*
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010982:Platelet-Derived Growth Factor; C075278:platelet-derived growth factor A