New biocompatible cationic amphiphiles derivative from glycine betaine: a novel family of efficient nonviral gene transfer agents.
With the aim of developing new efficient agents for transfecting of eukaryotic cells we have designed and synthesized a novel family of cationic lipid vectors derived from glycine betaine. In this study we present three novel molecules differing by the length of their aliphatic chains (R=12,R=14,R=16). The lyotropic properties of these cationic lipids have been determined, and their transfection efficiency on different cell lines evaluated, using a luminescent assay. Two of these compounds, GB14 and GB12 are efficient in vitro experiments. Cytoxicity evaluation of these new molecules showed promising results with a low cytotoxicity, especially when co-lipids were included in the formulation. These compounds represent a new family of gene transfer vectors which display good transfection efficiency and low toxicity, possibly due to the natural properties of glycine betaine. These compounds have great potential for the future development of in vivo gene transfer protocols.
Floch
V
V
Centre de Biogénétique, University, Hospital, ETSBO, Brest Cedex, 29275, France.
Legros
N
N
Loisel
S
S
Guillaume
C
C
Guilbot
J
J
Benvegnu
T
T
Ferrieres
V
V
Plusquellec
D
D
Ferec
C
C
D001622:Betaine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000633:toxicity; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000378:metabolism* / Q000633:toxicity; D002412:Cations / Q000633:toxicity; D018014:Gene Transfer Techniques; D019073:HT29 Cells; D006801:Humans; D020014:K562 Cells; D008081:Liposomes / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000235:genetics; D012996:Solutions; D018675:Toxicity Tests; D014162:Transfection
D001672:Biocompatible Materials; D002412:Cations; D008081:Liposomes; D010714:Phosphatidylethanolamines; D012996:Solutions; D001622:Betaine; C020888:1,2-dielaidoylphosphatidylethanolamine