Cellular expression of inducible nitric oxide synthase following rat cortical incision and its suppression by hydroxyl radical scavenger, 1,2-bis(nicotinamido)propane.
Cellular expression of an inducible isoform of nitric oxide synthase (iNOS) was studied immunohistochemically 1,3 and 7 days following rat cortical incision. The induction of iNOS was demonstrated almost exclusively in the macrophages accumulated within the incision cavity on day 3. They were significantly reduced in number by the treatment with 1,2-bis(nicotinamido)propane (P < 0.01), suggesting the involvement of hydroxyl radicals in the macrophage activation after cortical injury.
Yamanaka
K
K
Department of Neurosurgery, Osaka University Medical School, Suita, Japan.
Kumura
E
E
Yoshimine
T
T
Maruno
M
M
Taniguchi
M
M
Kosaka
H
H
Hayakawa
T
T
D000818:Animals; D000906:Antibodies; D001930:Brain Injuries / Q000201:enzymology*; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D008264:Macrophages / Q000502:physiology; D008297:Male; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D017207:Rats, Sprague-Dawley
D000906:Antibodies; D016166:Free Radical Scavengers; D009536:Niacinamide; D017665:Hydroxyl Radical; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat; C042625:nicaraven