Genotoxicity of the dust organic extract and its fractions derived from an aluminium electrolytic plant.
The dust derived from an aluminium electrolytic plant was collected on a filter, then extracted by mixed solvent of benzene, hexane and isopropanol (7/2/1, v/v). The solvent-soluble components was separated into five fractions, namely organic acids, organic alkalis, aliphatic hydrocarbons, polycyclic aromatic hydrocarbons and polar compounds. The genotoxicities of the dust organic extract and its five fractions were examined with Ames test, unscheduled DNA synthesis (UDS), micronucleus (MN) and sister-chromatid exchange (SCE) tests in human lymphocytes in vitro which involve the different test systems (bacteria and mammalian cells) and three genetic endpoints (gene mutation, chromosome aberration and DNA damage). The results of four experiments indicated that the dust organic extract showed higher genotoxicity. Among the five fractions, three fractions, namely organic acids, polycyclic aromatic hydrocarbons and polar compounds had higher genotoxicity than others. The other fractions, organic alkalis and aliphatic hydrocarbons had no genotoxicity. According to this study, it is necessary to take effective measures to abate the dust and protect the environment and human health.
Yumei
W
W
Department of Biology, Lanzhou University, GanSu, People's Republic of China.
Jinfeng
J
J
Xiaohong
Z
Z
Baoshan
Y
Y
D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000535:Aluminum / Q000633:toxicity*; D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004391:Dust / Q000032:analysis; D004595:Electroplating; D006801:Humans; D008669:Metallurgy; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D012854:Sister Chromatid Exchange / Q000187:drug effects
D000395:Air Pollutants, Occupational; D004391:Dust; D009930:Organic Chemicals; D004247:DNA; D000535:Aluminum