title: Targeting of acetylcholine receptor and 43 kDa rapsyn to the postsynaptic membrane in Torpedo marmorata electrocyte.
abstract: In this study we have investigated the intracellular routing of two major components of the postsynaptic membrane in Torpedo electrocytes, the nicotinic acetylcholine receptor and the extrinsic 43 kDa protein rapsyn, and of a protein from the non-innervated membrane, the Na+,K+ ATPase. We isolated subpopulations of post-Golgi vesicles (PGVs) enriched either in AChR or in Na+,K+ ATPase. Rapsyn was associated to AChR-containing PGVs suggesting that both AChR and rapsyn are targeted to intracellular organelles in the secretory pathway before delivery to the postsynaptic membrane. In vitro assays further show that rapsyn-containing PVGs do bind more efficiently to microtubules compared to Na+,K+ ATPase-enriched PVGs. These data provide evidence in favor of the contribution of the secretory pathway to the delivery of synaptic components.
authors.affiliation: Institut Jacques-Monod, CNRS, Universités Paris 6, France.
authors.forename: F
authors.initials: F
authors.lastname: Bignami
authors.forename: G
authors.initials: G
authors.lastname: Camus
authors.forename: S
authors.initials: S
authors.lastname: Marchand
authors.forename: L
authors.initials: L
authors.lastname: Bailly
authors.forename: F
authors.initials: F
authors.lastname: Stetzkowski-Marden
authors.forename: J
authors.initials: J
authors.lastname: Cartaud
mesh_terms: D000818:Animals; D004557:Electric Organ / Q000737:chemistry* / Q000166:cytology / Q000294:innervation; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D011950:Receptors, Cholinergic / Q000032:analysis*; D011978:Receptors, Nicotinic / Q000032:analysis*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013570:Synaptic Membranes / Q000737:chemistry*; D014101:Torpedo / Q000033:anatomy & histology / Q000378:metabolism*
chemical_list: D009124:Muscle Proteins; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; C051609:peripheral membrane protein 43K; D000254:Sodium-Potassium-Exchanging ATPase