Extraction and characterization of native canine bone morphogenetic protein (cBMP) qualified with osteoinductivity.
Bone morphogenetic protein (BMP) was extracted from canine bone matrix, partially purified and tested for osteoinductivity. A radiographically and histologically detectable ectopic bone formation was induced by 6.0 mg canine (cBMP) in muscle pouch of BALB mouse at 21 days post implantation. Characterization of the cBMP preparation by a gel filtration chromatography defined that the material consisted of proteins or protein complexes with molecular weights from 4 to 120 kD. Isoelectric focusing showed that the molecules were acidic with isoelectric points of 4.6-5.6.
Oksanen
J
J
Surgical Bioimplants Center, University of Tampere, Finland. Jarkko.Oksanen@uta.fi
Marttinen
A
A
Paatsama
S
S
Lindholm
T S
TS
D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D016723:Bone Remodeling / Q000187:drug effects*; D001842:Bone and Bones / Q000000981:diagnostic imaging; D002850:Chromatography, Gel; D004285:Dogs; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D010012:Osteogenesis / Q000187:drug effects; D011859:Radiography
D019485:Bone Morphogenetic Proteins