Control of expression of a gene encoding an extensin by phytochrome and a blue light receptor in spores of Adiantum capillus-veneris L.
In the present study, using a newly developed fluorescent differential display technique, we have carried out large-scale screening for genes whose expression was regulated by phytochrome and antagonistically by a blue light receptor in the spores of the fern Adiantum capillus-veneris L. Spores after imbibition were briefly irradiated with red, red/blue or blue light and collected 8 h after the irradiation. Total RNA was isolated from each sample and used to make cDNA with an oligo-dT primer. The cDNA was then used as a template for PCR with the oligo-dT primer and 80 arbitrary primers. The resulting PCR products were analyzed by an automated fluorescent DNA sequencer. Among 8000 displayed bands, we identified 15 up-regulated and four down-regulated bands by red light, and this red light effect was irreversibly reversed by blue light. We cloned one of the up-regulated cDNA fragments and used it to screen a cDNA library prepared from the spores. The isolated insert is predicted to encode Ser-(Pro)n repeats and showed homology with cell wall-associated extensins. The expression of this cDNA was induced 8 h after a red light treatment and the red light induction was photoreversibly prevented by far-red light and photoirreversibly by blue light. The mRNA of this gene was detectable 4 h after red light irradiation and gradually increased in germinating spores.
Uchida
K
K
Hitachi Advanced Research Laboratory, Saitama, Japan.
Muramatsu
T
T
Jamet
E
E
Furuya
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D056931:Cryptochromes; D016172:DNA Fingerprinting; D018744:DNA, Plant / Q000737:chemistry; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D006023:Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D017956:Photoreceptor Cells, Invertebrate; D010940:Plant Proteins / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000737:chemistry; D043562:Receptors, G-Protein-Coupled; D013170:Spores / Q000737:chemistry
D056931:Cryptochromes; D018744:DNA, Plant; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; D006023:Glycoproteins; D010940:Plant Proteins; D018749:RNA, Plant; D043562:Receptors, G-Protein-Coupled; C119828:cry protein, Drosophila; C005488:extensin protein, plant