Epitope analysis of staphylococcal enterotoxin A using different synthetic peptides.
The antigenic determinants (or epitopes) of staphylococcal enterotoxin A (SEA) were analyzed using synthetic peptides and rabbit antibodies to their corresponding peptides. Of 12 different synthetic peptides tested, peptides A-1 (corresponding to the amino acid sequence 1-20), A-5 (81-100), and A-8 (141-160) were reactive with anti-SEA antibodies. However, all synthetic peptides were found to elicit antibodies reactive with native SEA molecule. These findings suggest that native SEA molecule contains at least 3 different antigenic determinants.
Hu
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DL
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Japan.
Imai
A
A
Ono
K
K
Sasaki
S
S
Nakane
A
A
Sugii
S
S
Shinagawa
K
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis*; D004768:Enterotoxins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000032:analysis*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D013211:Staphylococcus aureus / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004768:Enterotoxins; D000939:Epitopes; D010446:Peptide Fragments; C026066:enterotoxin A, Staphylococcal