p21 expression and mutation in gastric carcinoma: analysis by immunohistochemistry and PCR-SSCP.
p21 protein has been reported to be a critical downstream effector of p53 and a potent inhibitor of cyclin-dependent kinases. Thus, the p21 gene is thought to play a central role in tumor suppression. In this study we investigated p21 protein expression and mutation in gastric adenocarcinoma. A total of 76 primary gastric carcinoma specimens were immunohistochemically stained for p21 protein expression and evaluated the correlations between p21 expression and clinicopathologic features. In a proportion of them (20 cases), we also analyzed the possible presence of p21 gene mutations using PCR-SSCP method. Fourty seven out of 76 cases (61.8%) were p21-negative, and the remaining twenty nine cases (38.2%) were p21 -positive on immunostains. There was a correlation between the expression of p21, and the depth of tumor invasion and lymph node metastasis (p<0.05). No mutation of the p21 gene was detected in all of 20 tumor tissues. These results suggest that the status of p21 expression may have prognostic value in gastric adenocarcinoma.
Park
Y E
YE
Department of Pathology, College of Medicine, Hallym University, Chun-chon, Korea. path@sun.hallym
Choi
K C
KC
Choi
Y H
YH
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000328:Adult; D000368:Aged; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D013194:Staining and Labeling; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins