Effects of Salmonella typhimurium infection and ofloxacin treatment on glucose and glutamine metabolism in Caco-2/TC-7 cells.
The effects of both Salmonella typhimurium infection and 5 mM ofloxacin treatment on 2 mM glutamine and 5 mM glucose metabolism in the enterocyte-like Caco-2/TC-7 cell line were studied. These cells utilized glutamine (212.07 +/- 16.75 [mean +/- standard deviation] nmol per h per 10(6) viable cells) and, to a lesser extent, glucose (139.63 +/- 11.52 nmol per h per 10(6) viable cells). Metabolism of these substrates in Caco-2/TC-7 cells resembled that in rat, pig, or human enterocytes. Infection by S. typhimurium C53-enhanced glucose and glutamine substrate utilization by 32 and 22%, respectively and enhanced glucose and glutamine substrate oxidation by eight- and twofold, respectively. These increases in glucose and glutamine metabolism (especially glucose metabolism) were due in part to the metabolism of intracellular bacteria and/or to the activation of cellular metabolism. Substrate metabolism (especially glucose metabolism) in C53-infected cells was partially reduced by treatment with ofloxacin. It was concluded that cellular fuel metabolism is stimulated at the earliest stage of infection (3 to 4 h) and that treatment with 5 mM ofloxacin does not completely restore substrate metabolism to the levels observed in uninfected cells, possibly because this treatment does not eradicate intracellular S. typhimurium completely.
Posho
L
L
Centre Hospitalier Universitaire Bichat-Claude Bernard, Institut National de la Santé et de la Recherche Médicale, Unité 13, Paris, France.
Delbos-Bocage
L
L
Gueylard
D
D
Farinotti
R
R
Carbon
C
C
D000890:Anti-Infective Agents / Q000494:pharmacology*; D018938:Caco-2 Cells; D002245:Carbon Dioxide / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D015242:Ofloxacin / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000502:physiology
D000890:Anti-Infective Agents; D005973:Glutamine; D002245:Carbon Dioxide; D015242:Ofloxacin; D005947:Glucose