title: [The evolution of resistance in a Culex quinquefasciatus strain starting from selection with the pyrethroid insecticide lambdacyhalothrin].
abstract: The resistance change to different insecticides in Culex quinquesfasciatus strain select at the laboratory with doses of pyrethroid lambdacyhalothrin that would cause a larva mortality of 90% were studied. It was attained an increase of the resistance to this insecticide of 144.5 times compared with the original level, and it was obtained a resistant strain (287x). There was an increase of the levels of resistance to methyl-pyrimifos (2.4 times), propoxur (6 times), DDT (5.2 times), clorpirifos (22 time), cypermethrin (67.5 times), and deltamethrin (20.2 times). The frequencies of the genes that codify for the elevated esterases enzymes and for the modified acetylcholinesterase reached their maximum value. Significant changes were observed in the phenotypes for esterases in the electrophoresis in polyacrylamide gels. It was detected synergism of DEF and PB with lambdacyhalothrin. Therefore, the elevated esterases and the esterases of multiple function may contribute to resistance.
authors.affiliation: Instituto de Medicina Tropical Pedro Kourí.
authors.forename: T
authors.initials: T
authors.lastname: González
authors.forename: J A
authors.initials: JA
authors.lastname: Bisset
authors.forename: C
authors.initials: C
authors.lastname: Díaz
authors.forename: M M
authors.initials: MM
authors.lastname: Rodríguez
authors.forename: L
authors.initials: L
authors.lastname: Diéguez
mesh_terms: D000110:Acetylcholinesterase / Q000235:genetics; D000818:Animals; D003465:Culex / Q000201:enzymology / Q000235:genetics; D004357:Drug Synergism; D005787:Gene Frequency; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000037:antagonists & inhibitors*; D007814:Larva; D007928:Lethal Dose 50; D009570:Nitriles; D010641:Phenotype; D011722:Pyrethrins / Q000037:antagonists & inhibitors*; D012641:Selection, Genetic
chemical_list: D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; D000110:Acetylcholinesterase; C037304:cyhalothrin