title: Screening cloned PCR fragments by restriction endonuclease finger-printing to obtain wild-type sequences.
authors.affiliation: Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA.
authors.forename: K
authors.initials: K
authors.lastname: Hagiwara
authors.forename: A A
authors.initials: AA
authors.lastname: Freeman
authors.forename: M G
authors.initials: MG
authors.lastname: McMenamin
authors.forename: C C
authors.initials: CC
authors.lastname: Harris
mesh_terms: D001419:Bacteria; D003001:Cloning, Molecular / Q000379:methods*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D008970:Molecular Weight; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D017422:Sequence Analysis, DNA; D051901:Smad4 Protein; D015534:Trans-Activators / Q000235:genetics
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; C497796:SMAD4 protein, human; D051901:Smad4 Protein; D015534:Trans-Activators; D004262:DNA Restriction Enzymes