Detección de Escherichia coli toxigénica (LT) mediante la reacción en cadena de la polimerasa [The detection of toxigenic Escherichia coli (LT) by the polymerase chain reaction].
In this paper it is described the detection enteroxigenic Escherichia coli LT (+). This method is based on the amplification of a DNA fragment of 400 pairs of bases by polymerase chain reaction (PRC). The oligonucleotides were designed by the authors and the characteristic patterns were observed when the samples were submitted to an electrophoresis in an Agarose gel at 2%. The PCR had positive results with the strains of Escherichia coli 0:149 K; 88 (LT+) collection and with 20 strains isolated from patients with acute diarrhea. Negative results were found in Escherichia coli 0:101 K:99 NM (ST+), Vibrio cholerae 01 and Aeromonas hydrophila.
Ramírez
M
M
Instituto de Medicina Tropical Pedro Kourí.
Monté
R J
RJ
Regue
M
M
Bravo
L
L
Maestre
J L
JL
García
B
B
D016980:Aeromonas hydrophila / Q000302:isolation & purification; D001483:Base Sequence; D002675:Child, Preschool; D003967:Diarrhea / Q000382:microbiology; D004768:Enterotoxins; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D014734:Vibrio cholerae / Q000302:isolation & purification
D004768:Enterotoxins