title: Expression of the P2X7-receptor subunit in neurons of the rat retina.
abstract: Despite the considerable evidence of signaling by extracellular nucleotides in other sensory systems, few studies have been undertaken in the eye. Molecular and immunohistochemical methods were used to demonstrate the expression and cellular localization of the P2X7 receptor subunit in the retina and choroid. RT-PCR was used for the detection of P2X7 subunit mRNA in the rat of different postnatal developmental stages (P23-P210) and revealed the presence of P2X7-mRNA in the retina, but not in the choroid. In the adult rat retina, immunolabelling for P2X7 was detected in a number of cells in the inner nuclear layer (INL) and ganglion cell layer (GCL), suggesting different types of amacrine cells and ganglion cells. These results demonstrate for the first time the expression of the P2X7 receptor in the mammalian retina and furthermore in distinct neuronal cell populations. Our data suggest that extracellular ATP may provide both neuromodulatory and trophic influences on visual processing.
authors.affiliation: Department of Otolaryngology, University of Tuebingen, Roentgenweg 11, 72076, Tuebingen, Germany.
authors.forename: U
authors.initials: U
authors.lastname: Brändle
authors.forename: K
authors.initials: K
authors.lastname: Kohler
authors.forename: T H
authors.initials: TH
authors.lastname: Wheeler-Schilling
mesh_terms: D000367:Age Factors; D000818:Animals; D002829:Choroid / Q000737:chemistry / Q000166:cytology / Q000254:growth & development; D015870:Gene Expression; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D018048:Receptors, Purinergic P2 / Q000032:analysis* / Q000096:biosynthesis*; D058486:Receptors, Purinergic P2X7; D012160:Retina / Q000737:chemistry / Q000166:cytology* / Q000254:growth & development; D012165:Retinal Ganglion Cells / Q000737:chemistry / Q000166:cytology
chemical_list: C546470:P2rx7 protein, rat; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7