Differential expression of Rb2/p130 and p107 in normal human tissues and in primary lung cancer.
Two proteins, p107 and pRb2/p130, which are structurally and functionally similar to the product of the retinoblastoma gene (pRb), were cloned by taking advantage of their ability to bind transforming proteins of DNA tumor viruses through a particular region called the "pocket domain." Like pRb, both proteins play a fundamental role in growth control. Using immunocytochemical techniques, we examined a variety of normal human tissues for the expression of pRb2/p130 and p107. Both proteins were expressed ubiquitously, although a different tissue distribution and/or level of expression were found in various organs. Terminally differentiated cells, such as neurons and skeletal muscle, showed high expression levels for Rb2/p130, whereas p107 was expressed at higher levels in other cell types such as epithelia of the breast and prostate. We then examined the expression pattern of Rb2/p130 in 158 specimens of human lung cancer and found an inverse correlation between the histological grading of the tumors, the development of metastasis, and the level of expression of Rb2/p130.
Baldi
A
A
Departments of Pathology, Institute for Cancer Research and Molecular Medicine, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA.
Esposito
V
V
De Luca
A
A
Fu
Y
Y
Meoli
I
I
Giordano
G G
GG
Caputi
M
M
Baldi
F
F
Giordano
A
A
D001773:Blood Cells / Q000378:metabolism; D001923:Brain Chemistry; D001940:Breast / Q000378:metabolism; D002454:Cell Differentiation; D004064:Digestive System / Q000378:metabolism; D004702:Endocrine Glands / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D016161:Genes, Retinoblastoma; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D005810:Multigene Family; D009124:Muscle Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D018482:Muscle, Skeletal / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D009928:Organ Specificity; D010525:Peripheral Nerves / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D011467:Prostate / Q000378:metabolism; D011506:Proteins; D012137:Respiratory System / Q000378:metabolism; D050720:Retinoblastoma-Like Protein p107; D050717:Retinoblastoma-Like Protein p130; D014551:Urinary Tract / Q000378:metabolism
D009124:Muscle Proteins; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins; D010750:Phosphoproteins; D011506:Proteins; C493844:RBL1 protein, human; C493837:RBL2 protein, human; D050720:Retinoblastoma-Like Protein p107; D050717:Retinoblastoma-Like Protein p130