title: Determination of the affinity and kinetic constants for the interaction between the human virus echovirus 11 and its cellular receptor, CD55.
abstract: The biochemical properties of the molecular interactions mediating viral-cell recognition are poorly characterized. In this study, we use surface plasmon resonance to study the affinity and kinetics of the interaction of echovirus 11 with its cellular receptor decay-accelerating factor (CD55). As reported for interactions between cell-cell recognition molecules, the interaction has a low affinity (KD approximately 3.0 microM) as a result of a very fast dissociation rate constant (kon approximately 10(5) M-1.s-1, koff approximately 0.3 s-1). This contrasts with the interaction of soluble ICAM-1 (sICAM-1, CD54) with human rhinovirus 3 which has been reported to have a similar affinity but 10(2)-10(3)-fold slower kinetics (Casasnovas, J. M., and Springer, T. A. (1995) J. Biol. Chem. 270, 13216-13224). The extracellular portion of decay-accelerating factor comprises four short consensus repeat domains (domains 1-4) and a mucin-like stalk. By comparison of the binding affinity for echovirus 11 of various fragments of decay-accelerating factor, we are able to conclude that short consensus repeat domain 3 contributes approximately 80% of the binding energy.
authors.affiliation: Laboratory of Molecular Biophysics, Department of Biochemistry, University of Oxford, Rex Richards Building, South Parks Road, Oxford OX1 3QU, United Kingdom. susan@biop.ox.ac.uk
authors.forename: S M
authors.initials: SM
authors.lastname: Lea
authors.forename: R M
authors.initials: RM
authors.lastname: Powell
authors.forename: T
authors.initials: T
authors.lastname: McKee
authors.forename: D J
authors.initials: DJ
authors.lastname: Evans
authors.forename: D
authors.initials: D
authors.lastname: Brown
authors.forename: D I
authors.initials: DI
authors.lastname: Stuart
authors.forename: P A
authors.initials: PA
authors.lastname: van der Merwe
mesh_terms: D015374:Biosensing Techniques; D018958:CD55 Antigens / Q000378:metabolism*; D002460:Cell Line; D016384:Consensus Sequence; D029822:Enterovirus B, Human / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008562:Membrane Glycoproteins / Q000378:metabolism*; D010843:Pichia; D011485:Protein Binding; D011991:Receptors, Virus / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D018958:CD55 Antigens; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011994:Recombinant Proteins; C074958:echovirus 11 receptor