[Alteration of inositol phosphate metabolism in aortic smooth muscle cells from spontaneously hypertensive rat].
The changes of inositol phosphates in quiescent and GTP gamma S activated vascular smooth muscle cells (VSMC) derived from aorta of spontaneously hypertensive rat (SHR) were examined. The inositol phosphate assay revealed that there was no difference in the inositol-1, 4, 5-triphosphate (InsP3) level between the quiescent VSMCs of SHR and that of normotensive Wistar-Kyoto rats (WKY). G-protein activator GTP gamma S caused a dose- and time-dependent increase in the InsP3 level in VSMCs. Activated G proteins increased significantly the InsP3 level in VSMCs of SHR. In conclusion, the highly sensitive reactivity to GTP gamma S in the generation of InsP3 in VSMCs from SHR may be related to the possible abnomality of the number, substructure or function of some components in G proteins, phosphoinositide turnover.
Hu
S J
SJ
Cardiovascular Department, First Affiliated Hospital, Zhejiang Medical University, Hangzhou.
Huang
Y W
YW
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D007295:Inositol Phosphates / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D007295:Inositol Phosphates; D015544:Inositol 1,4,5-Trisphosphate; D019204:GTP-Binding Proteins