Multiple promoters direct stimulus and temporal specific expression of brain-derived neurotrophic factor in the somatosensory cortex.
Neuronal activity rapidly induces expression of brain-derived neurotrophic factor (BDNF) in the adult rat cortex. The rat BDNF gene has four differentially regulated promoters, each of which produce an mRNA containing a unique 5' exon (I-IV) and a common 3' exon (V) that encodes the mature BDNF protein. The present study used an exon-specific RT-PCR analysis to determine the time course of the induction from both seizures and whisker stimulation. Our data show that specific promoters are utilized at different stages of the activity-dependent induction of the BDNF gene. Furthermore, the data show a differential utilization of the four promoters following a specific stimulus.
Nanda
S
S
Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI 53705, USA.
Mack
K J
KJ
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000235:genetics*; D005786:Gene Expression Regulation; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012640:Seizures / Q000139:chemically induced / Q000378:metabolism; D013003:Somatosensory Cortex / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic; D014738:Vibrissae / Q000502:physiology
D019208:Brain-Derived Neurotrophic Factor; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger