Induction of an intronic enhancer of the human ciliary neurotrophic factor receptor (CNTFRalpha) gene by the TR3 orphan receptor.
A hormone response element, CNTFRalpha-NBRE (5'-AAAGGTCA-3') has been identified in the fifth intron of the alpha component of ciliary neurotrophic factor receptor gene (CNTFR-15) for the human TR3 orphan receptor (TR3). A specific binding between in vitro expressed TR3 and CNTFRalpha-NBRE was demonstrated by using electrophoretic mobility shift assay. A reporter gene assay using chloramphenicol acetyl-transferase (CAT) showed that CNTFR-15 has an enhancer activity that could be induced by TR3 in a dose-dependent manner. This induction was significantly reduced in the absence of CNTFRalpha-NBRE. Together, these results indicate CNTFRalpha-NBRE is sufficient to mediate TR3 action in inducing the enhancer activity of CNTFR-15. Our finding may, therefore, suggest CNTFRalpha is a target gene regulated by TR3 and expand the role of TR3 in the nervous system.
Mu
X M
XM
Department of Pathology, University of Rochester, NY 14642, USA.
Young
W J
WJ
Liu
Y X
YX
Uemura
H
H
Chang
C
C
D020934:Ciliary Neurotrophic Factor; D004268:DNA-Binding Proteins / Q000235:genetics*; D004742:Enhancer Elements, Genetic / Q000235:genetics; D005786:Gene Expression Regulation / Q000502:physiology*; D018390:Gene Targeting; D006801:Humans; D009419:Nerve Tissue Proteins / Q000502:physiology; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D020794:Receptor Protein-Tyrosine Kinases / Q000235:genetics*; D020801:Receptor, Ciliary Neurotrophic Factor; D018160:Receptors, Cytoplasmic and Nuclear; D017475:Receptors, Nerve Growth Factor / Q000235:genetics*; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors / Q000235:genetics*
D020934:Ciliary Neurotrophic Factor; D004268:DNA-Binding Proteins; C539821:NR4A1 protein, human; D009419:Nerve Tissue Proteins; D057105:Nuclear Receptor Subfamily 4, Group A, Member 1; D020801:Receptor, Ciliary Neurotrophic Factor; D018160:Receptors, Cytoplasmic and Nuclear; D017475:Receptors, Nerve Growth Factor; D011987:Receptors, Steroid; D011988:Receptors, Thyroid Hormone; D014157:Transcription Factors; D020794:Receptor Protein-Tyrosine Kinases