title: hpttg, a human homologue of rat pttg, is overexpressed in hematopoietic neoplasms. Evidence for a transcriptional activation function of hPTTG.
abstract: We have isolated a human cDNA clone encoding a novel protein of 22 kDa that is a human counterpart of the rat oncoprotein PTTG. We show that the corresponding gene (hpttg) is overexpressed in Jurkat cells (a human T lymphoma cell line) and in samples from patients with different kinds of hematopoietic malignancies. Analysis of the sequence showed that hPTTG has an amino-terminal basic domain and a carboxyl-terminal acidic domain, and that it is a proline-rich protein with several putative SH3-binding sites. Subcellular fractionation studies show that, although hPTTG is mainly a cytosolic protein, it is partially localized in the nucleus. In addition we demonstrate that the acidic carboxyl-terminal region of hPTTG acts as a transactivation domain when fused to a heterologous DNA binding domain, both in yeast and in mammalian cells.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Domínguez
authors.forename: F
authors.initials: F
authors.lastname: Ramos-Morales
authors.forename: F
authors.initials: F
authors.lastname: Romero
authors.forename: R M
authors.initials: RM
authors.lastname: Rios
authors.forename: F
authors.initials: F
authors.lastname: Dreyfus
authors.forename: M
authors.initials: M
authors.lastname: Tortolero
authors.forename: J A
authors.initials: JA
authors.lastname: Pintor-Toro
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D019337:Hematologic Neoplasms / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000235:genetics; D064249:Securin; D014158:Transcription, Genetic; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D009363:Neoplasm Proteins; D064249:Securin; C578002:pituitary tumor-transforming protein 1, human; D001616:beta-Galactosidase