title: Engineering of N-terminal threonines in the D1 protein impairs photosystem II energy transfer in Synechocystis 6803.
abstract: Mutants of the cyanobacterium Synechocystis sp. PCC 6803 with N-terminal changes in the photosystem (PSII) II D1 protein were analysed by flash-induced oxygen evolution, chlorophyll a fluorescence decay kinetics and 77 K fluorescence emission spectra. The data presented here show that mutations of the Thr-2, Thr-3 and Thr-4 in D1 do not influence the oxygen evolution. A perturbation on the acceptor side was observed and the importance of the N-terminal threonines for an efficient energy transfer between the phycobilisome and PSII and for stability of the PSII complex was demonstrated.
authors.affiliation: Department of Biochemistry, Stockholm University, Sweden.
authors.forename: C
authors.initials: C
authors.lastname: Funk
authors.forename: W P
authors.initials: WP
authors.lastname: Schröder
authors.forename: G
authors.initials: G
authors.lastname: Salih
authors.forename: R
authors.initials: R
authors.lastname: Wiklund
authors.forename: C
authors.initials: C
authors.lastname: Jansson
mesh_terms: D019943:Amino Acid Substitution; D002734:Chlorophyll / Q000378:metabolism; D000077194:Chlorophyll A; D000458:Cyanobacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D003624:Darkness; D004735:Energy Transfer; D007700:Kinetics; D008027:Light; D045342:Light-Harvesting Protein Complexes; D010100:Oxygen / Q000378:metabolism; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D045524:Phycobilisomes; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013912:Threonine
chemical_list: D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D045524:Phycobilisomes; D011994:Recombinant Proteins; D002734:Chlorophyll; D013912:Threonine; D010100:Oxygen; D000077194:Chlorophyll A