Chaperone-like activity of tubulin.
Tubulin, a ubiquitous protein of eukaryotic cytoskeleton, is a building block unit of microtubule. Although several cellular processes are known to be mediated through the tubulin-microtubule system, the participation of tubulin or microtubule in protein folding pathway has not yet been reported. Here we show that goat brain tubulin has some functions and features similar to many known molecular chaperones. Substoichiometric amounts of tubulin can suppress the non-thermal and thermal aggregation of a number of unrelated proteins such as insulin, equine liver alcohol dehydrogenase, and soluble eye lens proteins containing beta- and gamma-crystallins. This chaperone-like activity of tubulin becomes more pronounced as temperature increases. Aging of tubulin solution at 37 degreesC also enhances its chaperone-like activity. Tubulin loses its chaperone-like activity upon removal of its flexible hydrophilic C-terminal tail. These results suggest that both electrostatic and hydrophobic interactions are important in substrate binding by tubulin and that the negatively charged C-terminal tails play a crucial role for its chaperone-like activity.
Guha
S
S
Department of Biochemistry, Bose Institute, Calcutta 700054, India.
Manna
T K
TK
Das
K P
KP
Bhattacharyya
B
B
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D003459:Crystallins / Q000378:metabolism; D006041:Goats; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D008970:Molecular Weight; D011487:Protein Conformation; D017510:Protein Folding; D014404:Tubulin / Q000378:metabolism*
D003459:Crystallins; D007328:Insulin; D018832:Molecular Chaperones; D014404:Tubulin; D000426:Alcohol Dehydrogenase