title: Nuclear localization of the functional Bel 1 transactivator but not of the gag proteins of the feline foamy virus.
abstract: Interactions between host cells and foamy or spumaretroviruses are different from those of other known retroviruses. Previous work has suggested that the Gag and high-affinity DNA-binding Bel 1 transactivator of human foamy virus are localized in the nuclei of infected cells. Using two independent detection methods, we show here that the functionally active Bel 1 transactivator protein of feline foamy virus is of nuclear localization. In contrast to that reported for the human foamy virus Gag protein, the cat foamy virus Gag proteins exclusively localized in the cytoplasm close to perinuclear regions.
authors.affiliation: Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, 69009, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Bodem
authors.forename: M
authors.initials: M
authors.lastname: Zemba
authors.forename: R M
authors.initials: RM
authors.lastname: Flügel
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000032:analysis; D002415:Cats; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D015683:Gene Products, gag / Q000378:metabolism*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007668:Kidney; D008164:Luminescent Proteins; D058619:Maltose-Binding Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012191:Retroviridae Proteins / Q000378:metabolism*; D016092:Spumavirus / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014162:Transfection
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D015683:Gene Products, gag; D008164:Luminescent Proteins; D058619:Maltose-Binding Proteins; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D012191:Retroviridae Proteins; D015534:Trans-Activators; C074061:bel1 protein, Human foamy virus; D049452:Green Fluorescent Proteins