Study on oxygen supply and protection of bone marrow in acute radiation injured mice.
After irradiated by 8 Gy 60Co gamma-ray, mice were intraperitoneally injected immediately with 0.2 ml 100% compound blood-activating soup twice a day for 10 days. The in situ ulnar bone marrow partial pressure of oxygen (PbO2) was determined in vivo before, during and after irradiation respectively. The bone marrow sections in the same part were observed. Our results showed that the normal murine ulnar PbO2 was 12.72 +/- 1.05 kPa. During irradiation, the level of PbO2 decreased to 10.78 +/- 1.17 kPa (P < 0.001). And 3 days after irradiation, PbO2 decreased to 9.75 +/- 0.52 kPa, suggesting that the commonly used "blood-activating and stasis-eliminating" Chinese drugs could promote the rehabilitation and proliferation of bone marrow microvessels in the acute radiation injured mice, expand their areas, increase the oxygen supply of bone marrow microenvironment, thereby leading to PbO2 much higher increase than that of control group. It is also helpful in the proliferation and rehabilitation of hematopoietic cells.
Sun
H
H
Department of Internal Medicine, Tongji Hospital, Tongji Medical University, Wuhan.
Liu
W
W
Xiao
K
K
Dong
L
L
He
M
M
Hu
Y
Y
Shen
A
A
Jiang
Q
Q
D000818:Animals; D001785:Blood Gas Monitoring, Transcutaneous; D001853:Bone Marrow / Q000098:blood supply*; D002196:Capillaries / Q000187:drug effects; D003037:Cobalt Radioisotopes; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D008297:Male; D051379:Mice; D010101:Oxygen Consumption; D011833:Radiation Injuries, Experimental / Q000097:blood / Q000503:physiopathology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*
D003037:Cobalt Radioisotopes; D004365:Drugs, Chinese Herbal; D011837:Radiation-Protective Agents