Evaluation of parenchymal and nonparenchymal cell injury after different conditions of storage and reperfusion.
We used the isolated perfused rat liver model (IPRL) to assess parenchymal and nonparenchymal cell integrity after different conditions of storage and reperfusion. Two studies were performed. In study 1, the IPRL was applied to evaluate the effects of 30 min of normothermic reperfusion with Elohes solution, enriched William's medium (Wif), or Carolina rinse solution (CRS) following 24 h of cold preservation in high-K+ or high-Na+ UW solution. As indicated by creatine kinase-BB (CK-BB) release, reperfusion with CRS provided greater protection of endothelial cells after storage in high-K+ UW solution than after storage in high-Na+ UW solution. In study 2, livers were cold-preserved (24 h, 4 degrees C) in either high-K+ or high-Na+ UW solution, then flushed with either CRS or Wif solution at room temperature before reperfusion (120 min, 37 degrees C) with 5% albumin-William's medium E. There was no statistical difference between the rinse solutions for bile flow and transaminases release. However, CRS improved bile indocyanine green excretion, which is known to be a marker of parenchymal and nonparenchymal cell integrity. Therefore, we can assume that this rinse solution protects rat liver grafts from reperfusion-induced microvascular damage.
Abdennebi
H B
HB
Laboratoire de Recherches Chirurgicales, INSERM U.281, Hôpital Edouard Herriot, Lyon, France.
Steghens
J P
JP
Margonari
J
J
Ramella-Virieux
S
S
Barbieux
A
A
Boillot
O
O
D000818:Animals; D003402:Creatine Kinase / Q000378:metabolism; D015925:Cryopreservation; D004730:Endothelium, Vascular / Q000201:enzymology / Q000473:pathology; D007527:Isoenzymes; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D008111:Liver Function Tests; D008297:Male; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology / Q000473:pathology*
D007527:Isoenzymes; D019188:Organ Preservation Solutions; D003402:Creatine Kinase