Automated measurement of serum ferroxidase activity.
A method is described for automated measurement of serum ceruloplasmin ferroxidase activity. In this method, Fe2+ ions are used as the substrate. In addition, a new calibration system without ceruloplasmin is also presented. Optimum assay reaction conditions were determined. Maximal catalytic activity was obtained at 0.45 mol/L acetate buffer, pH 5.8. The reagents and calibrator are stable for at least 6 months. Significant correlations between serum ferroxidase and p-phenylenediamine oxidase activities (r = 0.96; P <0.0001) and copper concentration (r = 0.93; P <0.0001) were found. The range for serum ceruloplasmin ferroxidase activity in healthy persons was 198-1107 U/L, and in patients with bronchial asthma it was 601-1912 U/L.
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Department of Clinical Biochemistry, Faculty of Medicine, Research Hospital, Harran University, Sanliurfa, Turkey. ereloz@doruk.net.tr
D001249:Asthma / Q000097:blood; D001322:Autoanalysis / Q000379:methods / Q000592:standards; D002138:Calibration; D002570:Ceruloplasmin / Q000032:analysis* / Q000378:metabolism; D003300:Copper / Q000097:blood; D006527:Hepatolenticular Degeneration / Q000097:blood; D006801:Humans; D016780:Malaria, Vivax / Q000097:blood; D010084:Oxidation-Reduction; D010655:Phenylenediamines / Q000378:metabolism; D012016:Reference Values; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D010655:Phenylenediamines; D003300:Copper; D002570:Ceruloplasmin; C029728:4-phenylenediamine