Altered frequency of a promoter polymorphic allele of the kinin B1 receptor gene in inflammatory bowel disease.
OBJECTIVE
Evidence of kinin-mediated inflammation in the gastrointestinal tract is accumulating. The genes and some polymorphic sites have been characterized for both kinin B1 and B2 receptors. These candidate genes were studied for their possible association with inflammatory bowel disease (IBD).


METHODS
In a retrospective study, the prevalence of allele pairs for four polymorphic sites of the two kinin receptor genes was determined in 53 patients with IBD and in 110 healthy volunteers similar in age, body weight, and gender proportions, using polymerase chain reaction and other techniques.


RESULTS
Only the B1 receptor promoter polymorphism (G-699-->C) exhibited a significantly different allele frequency between the two groups (prevalence of the C allele of 5.7% in patients with IBD compared with 33.6% in controls; P = 0.0002) or between the controls and either etiologic subgroup (ulcerative colitis and Crohn's disease). Allelic polymorphisms affecting exon 3 of the B1 receptor gene (A1098-->G) or exon 2 (C181-->T) or 1 (a 9-base pair deletion) of the B2 receptor gene were found to be neutral.


CONCLUSIONS
The gene corresponding to the B1 receptor for kinins may be a nonetiologic marker of symptomatic IBD, as suggested by the altered prevalence of a polymorphism presumably affecting its regulation.
Bachvarov
D R
DR
Centre de Recherche, Pavillon l'Hôtel-Dieu de Québec, Centre Hospitalier Universitaire de Québec, Québec, Canada. dimchob@rsvs.ulaval.ca
Landry
M
M
Houle
S
S
Paré
P
P
Marceau
F
F
D000328:Adult; D000483:Alleles; D003093:Colitis, Ulcerative / Q000235:genetics*; D003424:Crohn Disease / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin / Q000235:genetics*
D043783:Receptor, Bradykinin B1; D018002:Receptors, Bradykinin