title: In vivo delayed-type hypersensitivity skin test anergy in human immunodeficiency virus type 1 infection is associated with T cell nonresponsiveness in vitro.
abstract: In a cross-sectional study, the prevalence of delayed-type hypersensitivity skin test anergy (DTHA) was examined in 136 asymptomatic human immunodeficiency virus-infected participants in relation to immunologic and virologic parameters. DTHA was assessed with a multitest cell-mediated immunity skin test. Of the 136 participants, with a mean CD4 T cell count of 335 x 10(6)/L, 25 were anergic (18.4%). In the stepwise forward multivariate logistic regression models, after adjustment for CD4 T cell counts, depending on whether it was analyzed continuously or after dichotomization (20th percentile), both T cell reactivity to CD2 plus CD28 antibodies or to CD3 antibodies were the most predictive markers of DTHA (odds ratio, 0.80; 95% confidence interval, 0.67-0.94; and odds ratio, 2.97; 95% confidence interval, 1.1-8.3, respectively). This study shows a strong correlation between the decreased T cell responses in vitro and DTHA. Therefore, next to DTHA testing, T cell function assays may be useful to test immune reconstitution observed during antiretroviral treatment.
authors.affiliation: Department of Public Health and Environment, Academic Medical Centre, Amsterdam, The Nethrlands. jmaas@gggd.amsterdam.nl
authors.forename: J J
authors.initials: JJ
authors.lastname: Maas
authors.forename: M T
authors.initials: MT
authors.lastname: Roos
authors.forename: I P
authors.initials: IP
authors.lastname: Keet
authors.forename: E A
authors.initials: EA
authors.lastname: Mensen
authors.forename: A
authors.initials: A
authors.lastname: Krol
authors.forename: J
authors.initials: J
authors.lastname: Veenstra
authors.forename: P T
authors.initials: PT
authors.lastname: Schellekens
authors.forename: S
authors.initials: S
authors.lastname: Jurriaans
authors.forename: R A
authors.initials: RA
authors.lastname: Coutinho
authors.forename: F
authors.initials: F
authors.lastname: Miedema
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D015415:Biomarkers; D018791:CD4 Lymphocyte Count; D017635:Clonal Anergy; D015658:HIV Infections / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006716:Homosexuality; D006801:Humans; D006968:Hypersensitivity, Delayed; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D015415:Biomarkers