title: In vivo tissue pO2 measurements in hamster skinfold by recessed pO2 microelectrodes and phosphorescence quenching are in agreement.
abstract: OBJECTIVE
  Phosphorescence quenching has been used successfully to optically measure in vivo blood pO2 in the microvasculature. Optical measurements have also been made in some tissues, but it is not clear whether these results accurately reflect tissue pO2.
  
  
  METHODS
  Recessed pO2 microelectrodes and the phosphorescence quenching technique were used simultaneously to measure in vivo tissue pO2 in hamster skinfold. The optical window for phosphorescence quenching was focused around the tips of microelectrodes that were positioned in tissue regions at least 100 microns from large microvessels.
  
  
  RESULTS
  Mean tissue pO2 measured by recessed pO2 microelectrodes was 18.4 +/- 1.7 (SE) Torr, and mean tissue pO2 determined from the time course of phosphorescence decay was 18.8 +/- 2.0 Torr (no significant difference). The two tissue pO2 measurements agreed over a wide range, from 2 to 46 Torr (r = 0.93, 39 paired measurements from six sites in 3 animals). There was no systematic change in the microelectrode tissue pO2 during the period of light excitation used for the optical method.
  
  
  CONCLUSIONS
  Under the conditions of our study, sufficient amounts of porphyrin dye leaked from the vasculature and diffused into tissue, allowing accurate measurements of tissue pO2 by the phosphorescence quenching technique. Furthermore, the optical method did not deplete significant amounts of O2 from tissue during light excitation.
authors.affiliation: Department of Physiology, University of Pennsylvania, Philadelphia 19104-6068, USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Buerk
authors.forename: A G
authors.initials: AG
authors.lastname: Tsai
authors.forename: M
authors.initials: M
authors.lastname: Intaglietta
authors.forename: P C
authors.initials: PC
authors.lastname: Johnson
mesh_terms: D000818:Animals; D006224:Cricetinae; D005069:Evaluation Studies as Topic; D008163:Luminescent Measurements; D008647:Mesocricetus; D008652:Mesoporphyrins; D008665:Metalloporphyrins; D008833:Microcirculation / Q000378:metabolism; D008839:Microelectrodes; D010092:Oximetry / Q000295:instrumentation / Q000379:methods*; D010100:Oxygen / Q000032:analysis* / Q000097:blood / Q000378:metabolism; D011048:Polarography / Q000379:methods; D012867:Skin / Q000098:blood supply / Q000378:metabolism
chemical_list: D008652:Mesoporphyrins; D008665:Metalloporphyrins; C079625:palladium-meso-tetra(4-carboxyphenyl)porphine; D010100:Oxygen