title: atp Mutants of Escherichia coli fail to grow on succinate due to a transport deficiency.
abstract: Escherichia coli atp mutants, which lack a functional H+-ATPase complex, are capable of growth on glucose but not on succinate or other C4-dicarboxylates (Suc- phenotype). Suc+ revertants of an atp deletion strain were isolated which were capable of growth on succinate even though they lack the entire H+-ATPase complex. Complementation in trans with the yhiF gene suppressed the growth of the Suc+ mutants on succinate, which implicates the yhiF gene product in the regulation of C4-dicarboxylate metabolism. Indeed, when the E. coli C4-dicarboxylate transporter (encoded by the dctA gene) was expressed in trans, the Suc- phenotype of the atp deletion strain reverted to Suc+, which shows that the reason why the E. coli atp mutant is unable to grow aerobically on C4-dicarboxylates is insufficient transport capacity for these substrates.
authors.affiliation: Department of Molecular Cell Physiology, Faculty of Biology, Free University, Amsterdam, The Netherlands.
authors.forename: F C
authors.initials: FC
authors.lastname: Boogerd
authors.forename: L
authors.initials: L
authors.lastname: Boe
authors.forename: O
authors.initials: O
authors.lastname: Michelsen
authors.forename: P R
authors.initials: PR
authors.lastname: Jensen
mesh_terms: D001426:Bacterial Proteins; D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics; D002874:Chromosome Mapping; D003470:Culture Media; D029382:Dicarboxylic Acid Transporters; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D017353:Gene Deletion; D005816:Genetic Complementation Test; D016296:Mutagenesis; D010641:Phenotype; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism*; D019895:Transposases
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D003470:Culture Media; D029382:Dicarboxylic Acid Transporters; D013386:Succinates; C073786:dctA protein, Sinorhizobium meliloti; D019895:Transposases; D006180:Proton-Translocating ATPases