Localization of HuC (ELAVL3) to chromosome 19p13.2 by fluorescence in situ hybridization utilizing a novel tyramide labeling technique.
HuC is a neural-specific member of the Elav family of RNA-binding proteins. This highly conserved gene family plays a crucial role in neurogenesis, and HuC (HGMW-approved symbol ELAVL3) is expressed at an early stage of neural development. Using a novel tyramide fluorescence in situ hybridization (T-FISH) technique, we localized HuC to chromosome 19p13.2. This localization was confirmed by radiation hybrid mapping and coincides with that of HuR (HGMW-approved symbol ELAVL1), another elav family member. Dual T-FISH analysis with HuC and HuR probes, however, indicated distinct loci, with HuC being centromeric to HuR. This study demonstrates the utility of T-FISH in colocalizing two genes on the same chromosomal preparation using only biotinylated probes.
Van Tine
B A
BA
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, 35294, USA.
Knops
J F
JF
Butler
A
A
Deloukas
P
P
Shaw
G M
GM
King
P H
PH
D000954:Antigens, Surface / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D000067778:ELAV-Like Protein 3; D005456:Fluorescent Dyes; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics
D000954:Antigens, Surface; D017931:DNA Primers; D051959:ELAV Proteins; D000067780:ELAV-Like Protein 1; D000067778:ELAV-Like Protein 3; C098770:ELAVL1 protein, human; C490264:ELAVL3 protein, human; D005456:Fluorescent Dyes; D009419:Nerve Tissue Proteins; D016601:RNA-Binding Proteins