title: The plasminogen binding site of the C-type lectin tetranectin is located in the carbohydrate recognition domain, and binding is sensitive to both calcium and lysine.
abstract: Tetranectin, a homotrimeric protein belonging to the family of C-type lectins and structurally highly related to corresponding regions of the mannose-binding proteins, is known specifically to bind the plasminogen kringle 4 protein domain, an interaction sensitive to lysine. Surface plasmon resonance and isothermal calorimetry binding analyses using single-residue and deletion mutant tetranectin derivatives produced in Escherichia coli showed that the kringle 4 binding site resides in the carbohydrate recognition domain and includes residues of the putative carbohydrate binding site. Furthermore, the binding analysis revealed that the interaction is sensitive to calcium in addition to lysine.
authors.affiliation: Laboratory of Gene Expression, Department of Molecular and Structural Biology, University of Aarhus, DK-8000 Aarhus C, Denmark.
authors.forename: J H
authors.initials: JH
authors.lastname: Graversen
authors.forename: R H
authors.initials: RH
authors.lastname: Lorentsen
authors.forename: C
authors.initials: C
authors.lastname: Jacobsen
authors.forename: S K
authors.initials: SK
authors.lastname: Moestrup
authors.forename: B W
authors.initials: BW
authors.lastname: Sigurskjold
authors.forename: H C
authors.initials: HC
authors.lastname: Thogersen
authors.forename: M
authors.initials: M
authors.lastname: Etzerodt
mesh_terms: D001665:Binding Sites; D001798:Blood Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D002846:Chromatography, Affinity; D006801:Humans; D037181:Lectins, C-Type; D008239:Lysine / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D010958:Plasminogen / Q000378:metabolism*; D011485:Protein Binding; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D037181:Lectins, C-Type; D011994:Recombinant Proteins; C048648:tetranectin; D010958:Plasminogen; D008239:Lysine; D002118:Calcium