title: Two-component signal-transducing systems involved in stress responses and vancomycin susceptibility in Lactobacillus sakei.
abstract: Fragments of five rrp genes encoding response regulators (RRs) in Lactobacillus sakei were amplified by PCR using degenerate oligonucleotide primers. The five rrp genes were part of distinct loci that also comprised hpk genes encoding histidine protein kinases (HPKs). The putative RRs belonged to the OmpR-PhoB subclass of response regulators that consist of N-terminal receiver and C-terminal DNA-binding domains. The putative HPKs were members of the EnvZ-NarX family of orthodox histidine protein kinases which possess two transmembrane segments in a non-conserved N-terminal domain and a C-terminal cytoplasmic kinase domain. Insertional inactivation of the rrp genes indicated that the RRs are implicated in susceptibility to the glycopeptide antibiotic vancomycin, and to extreme pH, temperature and oxidative conditions.
authors.affiliation: Laboratoire de Recherche sur la Viandel and La boratoire de Genetique Microbiennez.
authors.forename: Franqoise
authors.initials: F
authors.lastname: Morel-Deville
authors.affiliation: Laboratoire de Recherche sur la Viandel and La boratoire de Genetique Microbiennez.
authors.forename: Franck
authors.initials: F
authors.lastname: Fauvel
authors.affiliation: lnstitut National de la Recherche AgronomiqueDomaine de Vilvert, 78352 Jouy-en-Josas CedexFrance.
authors.forename: Patrice
authors.initials: P
authors.lastname: Morel
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D005798:Genes, Bacterial; D005809:Genes, Regulator / Q000235:genetics* / Q000502:physiology; D000071677:Histidine Kinase; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress / Q000235:genetics; D010641:Phenotype; D011494:Protein Kinases / Q000737:chemistry / Q000235:genetics* / Q000502:physiology; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics*; D013696:Temperature; D014640:Vancomycin / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; D014640:Vancomycin; D006861:Hydrogen Peroxide; D011494:Protein Kinases; D000071677:Histidine Kinase