title: [L-arginine transport in cultured vascular smooth muscle cells of spontaneously hypertensive rats and effect of liposome on the transport].
abstract: The characteristics of L-arginine (L-Arg) transport in cultured aortic smooth muscle cells (SMC) of spontaneously hypertensive rat (SHR) and control WKY rats were studied and the effect of liposome as L-Arg carrier on the transport was investigated. The results showed that the L-Arg transport of SMC in SHR was obviously lower than that in WKY rats. Maximum transport velocity (Vmax) of high and low affinity in SHR were respectively 48% (P < 0.01) and 49% (P < 0.01) of WKY rat, while the michaelis constant (K(m)) showed no significant difference (P > 0.05). Increase of L-Arg transport induced by tumor necrosis factor-alpha (TNF alpha) in SMC of SHR was obviously lower than that in WKY rats (P < 0.01). The uptake of L-Arg increased 10 to 20 times in SMC when incubated with liposome encapsulated L-Arg (Liposome-L-Arg) than with free L-Arg. The transport velocity in SMC incubated with liposome-L-Arg showed no significant difference in SHR and WKY rats (P > 0.05). The transport of liposome-L-Arg in SMC was not affected by TNF alpha in both the types of rats. The above results indicate that there exists a functional disturbance in L-Arg transport in the SMC of SHR, but the L-Arg transport in SMC can be obviously enhanced when liposome is used as L-Arg carrier. Thus, it appears that liposome-L-Arg may have clinical perspective in the treatment of hypertension.
authors.affiliation: Institute of Cardiovascular Basic Research, Beijing Medical University.
authors.forename: X H
authors.initials: XH
authors.lastname: Yao
authors.forename: X Y
authors.initials: XY
authors.lastname: Lu
authors.forename: L Z
authors.initials: LZ
authors.lastname: Zhang
authors.forename: D D
authors.initials: DD
authors.lastname: Niu
authors.forename: L R
authors.initials: LR
authors.lastname: Gao
authors.forename: Y Z
authors.initials: YZ
authors.lastname: Pang
authors.forename: J Y
authors.initials: JY
authors.lastname: Su
authors.forename: C S
authors.initials: CS
authors.lastname: Tang
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism; D001120:Arginine / Q000378:metabolism*; D001692:Biological Transport; D002478:Cells, Cultured; D004337:Drug Carriers; D006973:Hypertension / Q000378:metabolism*; D008081:Liposomes; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D014409:Tumor Necrosis Factor-alpha; D001120:Arginine