title: Numerical abnormalities of chromosomes 1 and 10 in endometrial adenocarcinoma: fluorescence in situ hybridization analysis of 23 archival paraffin-embedded samples.
abstract: Retrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei isolated from formalin-fixed paraffin-embedded tissue. We examined 23 archival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosomes 1 and 10 were detected in 39% of the case analyzed, and the frequency of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involving chromosomes 1 and 10 with endometrial carcinoma, as reported by other studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation.
authors.affiliation: Istituto di Genetica Molecolare del CNR, Alghero, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Muresu
authors.forename: A
authors.initials: A
authors.lastname: Cossu
authors.forename: A M
authors.initials: AM
authors.lastname: Scarpa
authors.forename: E V
authors.initials: EV
authors.lastname: Volpi
authors.forename: P C
authors.initials: PC
authors.lastname: Rocca
authors.forename: A
authors.initials: A
authors.lastname: Pintus
authors.forename: M G
authors.initials: MG
authors.lastname: Tibiletti
authors.forename: F
authors.initials: F
authors.lastname: Tanda
authors.forename: M
authors.initials: M
authors.lastname: Pirastu
authors.forename: G
authors.initials: G
authors.lastname: Massarelli
mesh_terms: D000328:Adult; D000368:Aged; D000782:Aneuploidy; D018196:Carcinoma, Adenosquamous / Q000235:genetics*; D018269:Carcinoma, Endometrioid / Q000235:genetics*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008875:Middle Aged; D016612:Paraffin Embedding; D012189:Retrospective Studies
chemical_list: D005819:Genetic Markers