Comparisons of the NACP self-oligomerizations induced by Abeta25-35 in the presence of dicyclohexylcarbodiimide and N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline.
NACP, the precursor protein of the non-amyloid beta/A4 protein (Abeta) component of Alzheimer's disease (AD) amyloid, also known as alpha-synuclein was shown to undergo self-oligomerization only in the presence of a modified Abeta fragment (residues 25 35) by using a relatively hydrophobic coupling reagent, dicyclohexylcarbodiimide (DCCD). Since the oligomerization not only required a relatively high concentration of DCCD but also its efficiency was suppressed even at a slightly basic pH of 7.5, another coupling reagent called N-(ethoxycarbonyl)-2-ethoxy-1,2-dihydroquinoline (EEDQ) was examined in order to make use of this technique to access the functional aspects of NACP in vitro by exploring more accurate and reproducible reaction conditions. The EEDQ also gave rise to the NACP oligomerization only in the presence of Abeta25-35 among the variously modified Abeta peptides. The reagent was about three times more effective than DCCD in terms of its optimal concentration to visualize the oligomers. In addition, its oligomerizing potency was not affected by the basic condition. Although physiological and pathological significance of the NACP self-oligomerization are currently unknown, this dramatic phenomenon and its visualization technique could shed light on the determination of molecular relationships of NACP with various intracellular or extracellular biomolecules related to the pathological conditions of Alzheimer's and Parkinson's diseases.
Lee
J H
JH
Department of Biochemistry, College of Medicine, Inha University, Inchon, Korea.
Shin
H J
HJ
Chang
C S
CS
Paik
S R
SR
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D001704:Biopolymers; D003432:Cross-Linking Reagents; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D010446:Peptide Fragments / Q000494:pharmacology*; D010750:Phosphoproteins / Q000737:chemistry*; D011804:Quinolines / Q000494:pharmacology*; D012995:Solubility; D051843:Synucleins; D014867:Water / Q000737:chemistry; D051844:alpha-Synuclein
D016229:Amyloid beta-Peptides; D001704:Biopolymers; D003432:Cross-Linking Reagents; D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D010750:Phosphoproteins; D011804:Quinolines; D051843:Synucleins; D051844:alpha-Synuclein; C074636:amyloid beta-protein (25-35); D014867:Water; D004024:Dicyclohexylcarbodiimide; C000470:EEDQ