Promoter analysis of the acetate-inducible isocitrate lyase gene (acu-3) from Neurospora crassa.
Analysis of the promoter region of the acetate-induced isocitrate lyase gene (acu-3) of Neurospora crassa was undertaken. A series of deletions in the 5' non-transcribed region were constructed and the effects of these mutations on the enzyme levels following growth on sucrose and transfer to acetate were measured. Sequences within the region -603 to -271 relative to the transcription start site appear essential for transcription. The region -950 to -1278 is required for sucrose repression, which is consistent with previous protein/DNA gel retardation results of protein extracts from N. crassa cultured on sucrose. Protein extracts from acetate-induced mycelia identify alternative promoter regions apparently involved in acetate-induced gene transcription.
Bibbins
M
M
Department of Food and Macromolecular Science, Institute of Food Research, Earley Gate, Reading RG6 6BZ, UK.
Sheffield
P J
PJ
Gainey
L D
LD
Mizote
T
T
Connerton
I F
IF
D000085:Acetates / Q000494:pharmacology; D001483:Base Sequence; D004790:Enzyme Induction; D007522:Isocitrate Lyase / Q000096:biosynthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015183:Restriction Mapping; D017384:Sequence Deletion; D013395:Sucrose / Q000494:pharmacology; D014158:Transcription, Genetic
D000085:Acetates; D013395:Sucrose; D007522:Isocitrate Lyase