Diacetylamethystoidin A protects isolated working rat heart against myocardial reperfusion injury.
OBJECTIVE
To observe the effect of diacetylamethystoidin A (6 alpha, 13 beta-diacetyl-11, 15-dioxo-19-hydroxyl-kaurene, DAA-A) on myocardial ischemia reperfusion injury.


METHODS
Working heart mode was induced in isolated rat heart subjected to a 40-min ceasing perfusion followed by a 25-min reperfusion.


RESULTS
DAA-A 0.13, 0.25, 0.50 mmol.L-1 reduced the incidence of ventricular fibrillation (1, 1, 0 vs 4 in Vehicle and 5 in Control, respectively), increased the coronary flow (mL.min-1, 1.8 +/- 0.9, 1.9 +/- 0.8, 1.7 +/- 0.6 vs 1.0 +/- 0.3 in Vehicle and 0.9 +/- 0.5 in Control as reperfusion time 5 min, respectively), improved the contractile function of heart, decreased the release of lactic dehydrogenase (LDH) and malondialdehyde (MDA). DAA-A 0.25 mmol.L-1 reduced the injury of myocardial cell ultrastructure.


CONCLUSIONS
DAA-A has cardioprotective effect through diminishing cellular lipid peroxidation induced by oxygen free radicals.
Zhang
C F
CF
Department of Pharmacology, Jining Medical College, China.
Wang
D S
DS
Ling
X Z
XZ
D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D004224:Diterpenes / Q000494:pharmacology*; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D051381:Rats; D017208:Rats, Wistar; D014693:Ventricular Fibrillation / Q000517:prevention & control*
D000889:Anti-Arrhythmia Agents; D004224:Diterpenes; C116375:diacetylamethystoidin A; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase