Prognostic value of JC virus load in cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy.
JC virus (JCV) load was determined by using quantitative polymerase chain reaction in cerebrospinal fluid (CSF) of 12 patients with AIDS-associated progressive multifocal leukoencephalopathy (PML) and compared with clinical outcome. JCV loads varied widely (3-7 log10 JCV equivalents/mL of CSF) and were apparently not related to absolute CD4 cell counts or CSF and plasma human immunodeficiency virus type 1 loads. A significant correlation was observed between JCV load and survival time (Spearman's rank correlation, -0.83; P<. 01). Moreover, CSF JCV load decreased and then became undetectable in 1 PML patient receiving cidofovir treatment, and this was associated with clinical improvement. These results show that CSF JCV load may be useful as a prognostic parameter and in monitoring the effectiveness of anti-JCV therapies in PML patients.
Taoufik
Y
Y
Department of Microbiology, Paul Brousse Hosptial, Cedex, France. yassine.taoufik@pbr.ap-hop-paris.fr
Gasnault
J
J
Karaterki
A
A
Pierre Ferey
M
M
Marchadier
E
E
Goujard
C
C
Lannuzel
A
A
Delfraissy
J F
JF
Dussaix
E
E
D015526:AIDS Dementia Complex / Q000134:cerebrospinal fluid* / Q000503:physiopathology / Q000821:virology*; D017088:AIDS-Related Opportunistic Infections / Q000134:cerebrospinal fluid / Q000188:drug therapy; D000998:Antiviral Agents / Q000627:therapeutic use; D018791:CD4 Lymphocyte Count; D000077404:Cidofovir; D003596:Cytosine / Q000031:analogs & derivatives / Q000627:therapeutic use; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral / Q000134:cerebrospinal fluid; D005260:Female; D015497:HIV-1 / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification*; D008297:Male; D063065:Organophosphonates; D009943:Organophosphorus Compounds / Q000627:therapeutic use; D030361:Papillomavirus Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000209:etiology; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D018709:Statistics, Nonparametric; D014412:Tumor Virus Infections / Q000134:cerebrospinal fluid* / Q000188:drug therapy / Q000209:etiology; D019562:Viral Load
D000998:Antiviral Agents; D017931:DNA Primers; D015342:DNA Probes; D004279:DNA, Viral; D063065:Organophosphonates; D009943:Organophosphorus Compounds; D003596:Cytosine; D000077404:Cidofovir