Increase in odontoclast nuclei number by cell fusion: a three-dimensional reconstruction of cell fusion of human odontoclasts.
Osteoclasts and odontoclasts are known to increase their nuclear number by fusion of mononuclear precursors. However, the pattern of fusion remains morphologically unclear. One lower right deciduous canine of an 8-year-old male was investigated. Tartrate-resistant acid phosphatase activity (TRAP) positive cells on the resorbing surface of the tooth were serially sectioned into 0.5 microm-thick semithin sections. The sections were photographed, and cells possessing a light microscopic brush border facing a resorptive lacuna were identified as odontoclasts. Fourteen odontoclasts appearing as a continuous figure of cellular membrane between cells on one section were three-dimensionally reconstructed using NIKON COSMOZONE 2SA. A criterion for fusion was established in this study, requiring that there must be two or more nucleated cells which contacted each other at one site only in the three-dimensional reconstruction. Among 14 reconstructed cells, 10 odontoclasts satisfied the criterion for fusion. The observations of the three-dimensional structures of these odontoclasts showed that mononuclear and multinucleated odontoclasts participated in fusion. Cell fusion occurred between resorbing odontoclasts and cells not forming lacunae, and between resorbing odontoclasts. A case of odontoclastic fusion among three cells was also observed. The results establish that fusion resulting in multinucleation occurred among various odontoclasts with different numbers of nuclei including mononuclear odontoclasts.
Domon
T
T
Department of Oral Anatomy II, Hokkaido University School of Dentistry, Sapporo, Japan.
Yasuda
M
M
Osanai
M
M
Suzuki
R
R
Takahashi
S
S
Yamamoto
T
T
Wakita
M
M
D002459:Cell Fusion / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D002648:Child; D003481:Cuspid / Q000166:cytology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008297:Male; D008867:Microtomy; D010010:Osteoclasts / Q000166:cytology* / Q000648:ultrastructure