A novel strategy for unambiguous DPB1 typing.
A DPB1 typing method is described that assigns DPB1 alleles into six groups based on polymorphism at amino acid positions 8-9 and 84-87 using sequence-specific priming (SSP). The results obtained allow the selection of primers for a subsequent sequence-specific oligonucleotide (SSO) hybridization procedure which permits DPB1 alleles to be analysed separately in a heterozygote individual. This has greatly reduced the occurrence of typing reaction patterns consistent with multiple combinations of DPB1 alleles seen in other DPB1 typing methods.
Varney
M D
MD
Department of Pathology, Royal Melbourne Hospital, Parkville, VIC, Australia.
Tait
B D
BD
D006682:HLA-DP Antigens / Q000145:classification / Q000235:genetics*; D059868:HLA-DP beta-Chains; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
D006682:HLA-DP Antigens; D059868:HLA-DP beta-Chains; C071170:HLA-DPB1 antigen