[Receptors of monoamine in sympathetic preganglionic neurons of neonatal rat spinal cord in vitro].
By means of intracellular recordings from spinal cord slices of neonatal rats in vitro, the effects of 5-hydroxytryptamine (5-HT), nor-adrenaline (NA) and adrenaline (AD) on membrane potential in sympathetic preganglionic neurons (SPN) were observed, in order to clarify whether these neuron contain a single type of the monoamine receptor or in combination with more than one type of receptors. The results showed that: (1) 5-HT, NA and AD induced membrane depolarization respectively in 57.1% (16/28), 60% (15/25) and 52.4% (11/21) of SPN. (2) According to the reactions of SPN to the three monoamines, several subtypes of SPN could be divided: those sensitive to all the three monoamines (3/19), those sensitive to two of them (9/19), those only sensitive to one type of monoamines (4/19) and those insensitive at all (3/19). The significance of coexistence of more than one type of the three monoamines in a single neuron remains to be elucidated.
Cai
S R
SR
Department of Physiology, Anhui Medical University, Hefei.
Wang
L C
LC
Kong
D H
DH
Huang
Z X
ZX
Ma
R C
RC
D000818:Animals; D000831:Animals, Newborn; D001339:Autonomic Fibers, Preganglionic / Q000737:chemistry* / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000502:physiology; D051381:Rats; D011941:Receptors, Adrenergic / Q000032:analysis*; D011985:Receptors, Serotonin / Q000032:analysis*; D013116:Spinal Cord / Q000737:chemistry* / Q000502:physiology
D011941:Receptors, Adrenergic; D011985:Receptors, Serotonin