title: Identification of kinesin-like molecules in myogenic cells.
abstract: Numerous organelles are repositioned during myogenic differentiation and are maintained in an asymmetric distribution throughout the life span of a myotube. It is likely that members of the kinesin superfamily may be responsible for some or all of these microtubule-dependent movements. Consequently, we have attempted to identify kinesin-like molecules expressed throughout myogenesis. Using a standard PCR-based strategy, we cloned two kinesin-like molecules from a rat myogenic cell line, L6. Sequence analysis of the first of these, KIF3C, defines it as a novel member of the KIF3 subfamily of kinesin-like proteins. KIF3C is expressed throughout myogenesis as well as in numerous rat tissues. Like other members of the KIF3 subfamily, KIF3C has an N-terminal motor domain. The second molecule identified is a rat homolog of murine KIF1B, a putative mitochondrial transporter. KIF1B is also expressed ubiquitously both in myogenic cells at all stages and in a variety of rat tissues.
authors.affiliation: Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: K
authors.initials: K
authors.lastname: Faire
authors.forename: D
authors.initials: D
authors.lastname: Gruber
authors.forename: J C
authors.initials: JC
authors.lastname: Bulinski
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D016547:Kinesins / Q000235:genetics / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000235:genetics / Q000302:isolation & purification*; D018482:Muscle, Skeletal / Q000737:chemistry* / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D051381:Rats; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; C490399:Kif1b protein, rat; C484677:Kif3c protein, rat; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D016547:Kinesins