title: DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.
abstract: The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
authors.affiliation: Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington 98109, USA.
authors.forename: M S
authors.initials: MS
authors.lastname: Jurica
authors.forename: R J
authors.initials: RJ
authors.lastname: Monnat
authors.forename: B L
authors.initials: BL
authors.lastname: Stoddard
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D003461:Crystallography; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D007438:Introns; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; C048901:endodeoxyribonuclease CreI; D002118:Calcium