A reproducible model of thromboembolic stroke in mice.
AN experimental mouse model of thromboembolic stroke is presented, in which standardized fibrin-rich emboli (150 microm diameter, 1.5 mm or 4 mm length) are injected into the internal carotid artery. Injection of six or eight 1.5 mm clots (corresponding to 0.16 or 0.21 microl clot material) led to a variable decrease in laser Doppler flow (LDF), but did not result in reproducible infarcts of the middle cerebral artery (MCA) territory, as determined by triphenyltetrazolium chloride (TTC) staining 24 h after embolization. Injection of ten 1.5 mm clots (0.27 microl) or four 4 mm clots (0.28 microl), however, caused a persistent LDF decline to about 20-30% of baseline and led to reproducible infarcts covering the entire MCA territory. The method establishes a clinically relevant, reproducible stroke model for the study of molecular mechanisms of ischemic brain injury in genetically engineered mice.
Kilic
E
E
Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Cologne, Germany.
Hermann
D M
DM
Hossmann
K A
KA
D000818:Animals; D001921:Brain / Q000098:blood supply / Q000473:pathology; D002341:Carotid Artery Thrombosis / Q000473:pathology; D002343:Carotid Artery, Internal; D002561:Cerebrovascular Disorders / Q000473:pathology*; D004195:Disease Models, Animal; D004617:Embolism / Q000503:physiopathology; D002542:Intracranial Embolism and Thrombosis / Q000473:pathology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008953:Models, Anatomic; D015203:Reproducibility of Results; D013778:Tetrazolium Salts / Q000737:chemistry
D013778:Tetrazolium Salts; C009591:triphenyltetrazolium