Self-complementary oligodeoxyribonucleotides incorporating L-related isodeoxynucleosides: synthesis, physical characterization, enzymology, and CD studies.
The synthesis of self-complementary oligodeoxyribonucleotides (14-16, 18) incorporating the L-related isodeoxynucleotides, isodA (1) and/or isodT (2), is described. For this purpose, the fully protected phosphoramidite building blocks 10 and 12 were synthesized. The conceptually new oligomers exhibit high resistance towards exonucleases. Temperature- and concentration-dependent CD spectral data and accompanying thermodynamic parameters of the oligomers show that isodA-isodT or mixed dA-isodT/isodA-dT base pairs participate in duplex stabilization.
Wenzel
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Department of Chemistry, The University of Iowa, Iowa City, Iowa 52242, USA.
Nair
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D055598:Chemical Phenomena; D002627:Chemistry, Physical; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D018076:DNA, Complementary / Q000138:chemical synthesis / Q000737:chemistry*; D005092:Exonucleases / Q000378:metabolism; D006868:Hydrolysis; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000737:chemistry*; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature; D013816:Thermodynamics
D018076:DNA, Complementary; D007202:Indicators and Reagents; D009705:Nucleosides; D009838:Oligodeoxyribonucleotides; D005092:Exonucleases