Latex immunoassays: comparative studies on covalent and physical immobilization of antibodies. II. IgG.
Latex particles coated with IgG, currently used for immunoassay tests, are not colloidally stable under physiological conditions. Post-treatment of sensitized polystyrene microspheres with different substances (BSA, surfactants) to increase colloidal stability has been often used to solve this problem. We propose the possibility of stabilizing the antibody-latex conjugates by hydration forces at high ionic strength. On the other hand. immobilization of the proteins may be performed either by physical adsorption or by covalent binding to functionalized surface groups. In this second part of these series we have studied the immunoreactivity of IgG antibody partly covalently bound and physically adsorbed, and the results were compared to those obtained with F(ab')2 fragments in Part I.
Molina-Bolívar
J A
JA
Departamento de Física Aplicada, Facultad de Ciencias, Universidad de Granada, Spain.
Galisteo-González
F
F
Hidalgo-Alvarez
R
R
D000327:Adsorption; D002097:C-Reactive Protein / Q000737:chemistry / Q000276:immunology; D003102:Colloids; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000276:immunology; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D007841:Latex Fixation Tests; D008863:Microspheres; D009391:Nephelometry and Turbidimetry; D013997:Time Factors
D003102:Colloids; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D002097:C-Reactive Protein