title: Agrobacterium tumefaciens transformation of the radiation hypersensitive Arabidopsis thaliana mutants uvh1 and rad5.
abstract: The Arabidopsis thaliana mutants uvh1 and rad5, originally identified as radiation hypersensitive, were reported to be deficient in T-DNA integration based on the relative efficiencies of stable transformation and T-DNA transfer. We reassessed these mutants for susceptibility to transformation by Agrobacterium tumefaciens. The mutant rad5 showed a significant reduction in the efficiency of transient as well as stable transformation, compared with its wild-type progenitor. These data indicate that rad5 is blocked at a step in the transformation process prior to T-DNA integration. We additionally found, using both an in vitro root inoculation and an in vivo flower bolt inoculation assay, that the mutant uvh1 is as susceptible to A. tumefaciens-mediated transformation as is its wild-type progenitor, C10.
authors.affiliation: Department of Biological Sciences, Purdue University, West Lafayette, IN 47907-1392, USA.
authors.forename: J
authors.initials: J
authors.lastname: Nam
authors.forename: K S
authors.initials: KS
authors.lastname: Mysore
authors.forename: S B
authors.initials: SB
authors.lastname: Gelvin
mesh_terms: D000251:Adenosine Triphosphatases; D016960:Agrobacterium tumefaciens / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics* / Q000382:microbiology / Q000528:radiation effects; D004265:DNA Helicases; D005656:Fungal Proteins / Q000235:genetics*; D009154:Mutation; D011836:Radiation Tolerance / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014170:Transformation, Genetic
chemical_list: D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; D000251:Adenosine Triphosphatases; C076225:RAD5 protein, S cerevisiae; D004265:DNA Helicases