title: Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of toxoplasma gondii.
abstract: Ultrastructural localization of a P29 protein of Toxoplasma gondii was examined on thin sections by an immunogold technique using a P29 antigen-specific monoclonal antibody (5-241-178). Immunolocalization of the P29 protein in extracellular tachyzoites demonstrated that this antigen was present in the dense granules. Thus, we have identified this P29 antigen as the seventh protein (GRA7) to be localized to the dense granules of T. gondii. P29 immunolocalization in intracellular tachyzoites demonstrated association of this antigen with the parasite membrane complex, tubular elements of the intravacuolar network, and with the parasitophorous vacuolar membrane. Our immunolabeling data suggest trafficking of the P29 (GRA7) antigen from the dense granule via the intravacuolar network to the parasitophorous vacuolar membrane on invasion of the tachyzoite into the host cell. (J Histochem Cytochem 46:1411-1421, 1998)
authors.affiliation: Equipe 4 INSERM U.314 IFR 53, CHU Maison Blanche, Reims, France.
authors.forename: A
authors.initials: A
authors.lastname: Bonhomme
authors.forename: G T
authors.initials: GT
authors.lastname: Maine
authors.forename: A
authors.initials: A
authors.lastname: Beorchia
authors.forename: H
authors.initials: H
authors.lastname: Burlet
authors.forename: D
authors.initials: D
authors.lastname: Aubert
authors.forename: I
authors.initials: I
authors.lastname: Villena
authors.forename: J
authors.initials: J
authors.lastname: Hunt
authors.forename: L
authors.initials: L
authors.lastname: Chovan
authors.forename: L
authors.initials: L
authors.lastname: Howard
authors.forename: S
authors.initials: S
authors.lastname: Brojanac
authors.forename: M
authors.initials: M
authors.lastname: Sheu
authors.forename: J
authors.initials: J
authors.lastname: Tyner
authors.forename: M
authors.initials: M
authors.lastname: Pluot
authors.forename: J M
authors.initials: JM
authors.lastname: Pinon
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000032:analysis; D015153:Blotting, Western; D003594:Cytoplasmic Granules / Q000737:chemistry; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D015800:Protozoan Proteins / Q000032:analysis* / Q000276:immunology; D014122:Toxoplasma / Q000737:chemistry* / Q000648:ultrastructure
chemical_list: D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; C112573:GRA7 protein, Toxoplasma gondii; D015800:Protozoan Proteins