[In vitro stimulation of HTC hepatoma cell growth by recombinant human augmenter of liver regeneration (ALR)].
It was demonstrated that biologically active ALR could be expressed from its cDNA in transient expression experiments in cos-7 cells. The results showed that the cytosolic fraction from the transfected cells with constructed plasmids DNA stimulated of DNA synthesis of in vitro HTC cells in a dose dependent manner. This finding suggests that the HTC hepatoma cell line may be used as a target for bioassay of human ALR in vitro.
Yang
X M
XM
Beijing Institute of Radiation Medicine.
Hu
Z Y
ZY
Xie
L
L
Wu
Z Z
ZZ
Wu
C T
CT
He
F C
FC
D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D008115:Liver Regeneration; D010455:Peptides / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology*; D014162:Transfection; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D010455:Peptides; D011994:Recombinant Proteins; C073456:hepatic stimulator substance