[Typing of H. pylori strains isolated from patients with gastric diseases].
Helicobacter pylori, an organism recently described in association with gastritis and peptic lesions. The degree of relationship between these micro-organisms was studied on molecular level using SDS-PAGE protein profiles and immunoblot patterns. Study on a total of 112 lysates of H. pylori showed some differences in protein patterns of different H. pylori strains. However, the difference became more apparent when studied by immunoblot analysis. According to SDS-PAGE protein, strains isolated from gastric mucous of the patients were identified as types I-IV, while type IV can be further classified as sub-type IV-1 to IV-5 analysis of SDS-PAGE protein patterns proved to be an excellent method of characterizing those H. pylori, different from traditional methods.
Chen
J J
JJ
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Zhang
J Z
JZ
Jiang
X G
XG
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D005753:Gastric Mucosa / Q000382:microbiology; D005756:Gastritis / Q000382:microbiology*; D016480:Helicobacter pylori / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D013276:Stomach Ulcer / Q000382:microbiology*