DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).
The analysis of cattle MHC (BoLA) class I gene expression is an essential component of studies on immune responses and susceptibility to disease. International BoLA workshops have generated data and reagents that allow discrimination of class I molecules at the haplotype level, but progress has been limited by difficulties encountered in defining single alleles. Our aim in this study was to develop a DNA-based system for improved identification of expressed class I alleles, utilizing available cDNA sequences derived from cattle carrying a series of serologically defined class I specificities. This method has allowed more accurate typing of animals for expression of the class I genes present within a small number of haplotypes. The method has also reliably differentiated between allelic variants (identified by prior sequence analysis) and has split existing serological specificities. The data show that MHC class I genes in cattle are more polymorphic than demonstrated by serology and biochemical analysis.
Ellis
S A
SA
Institute for Animal Health, Compton Laboratory, UK.
Staines
K A
KA
Stear
M J
MJ
Hensen
E J
EJ
Morrison
W I
WI
D000483:Alleles; D000818:Animals; D002417:Cattle; D004247:DNA / Q000145:classification; D017931:DNA Primers; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D004247:DNA