title: Simultaneous determination of pteridines in multicomponent mixtures using derivative spectrophotometry and partial least-squares calibration.
abstract: Simple binary mixtures composed of very similar pteridines (neopterin and pterin) have been resolved by derivative spectrophotometry. Detection limits of 0.30 microgram ml-1 and 0.12 microgram ml-1 for pterin and neopterin, respectively, have been calculated. Also, different mixtures of pteridines considered as disease markers, such as pterin, neopterin, xanthopterin and isoxanthopterin, have been determined by using a partial least-squares (PLS-2) model. Calibration set containing 0-7 micrograms ml-1 for each component was used. The resolution of several mixtures and single determination was tested in artificial samples.
authors.affiliation: Department of Analytical Chemistry, University of Extremadura, Badajoz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Espinosa-Mansilla
authors.forename: I
authors.initials: I
authors.lastname: Durán-Merás
authors.forename: F
authors.initials: F
authors.lastname: Salinas
mesh_terms: D004338:Drug Combinations; D004867:Equipment Design; D016018:Least-Squares Analysis; D015394:Molecular Structure; D019798:Neopterin / Q000032:analysis; D011621:Pteridines / Q000032:analysis* / Q000737:chemistry; D011622:Pterins / Q000032:analysis; D013053:Spectrophotometry / Q000295:instrumentation / Q000379:methods*; D014976:Xanthopterin / Q000032:analysis
chemical_list: D004338:Drug Combinations; D011621:Pteridines; D011622:Pterins; C008217:isoxanthopterin; D019798:Neopterin; D014976:Xanthopterin