A 500-kb YAC and BAC contig encompassing the high-growth deletion in mouse chromosome 10 and identification of the murine Raidd/Cradd gene in the candidate region.
The mouse high growth (hg) gene was identified in a selection experiment for rapid growth. It produces a 30-50% increase in weight gain of homozygous individuals without resulting in obesity. Recently, hg was mapped to a deletion around marker D10Mit69. Here we report a map of yeast artificial chromosome and bacterial artificial chromosome clones spanning the entire region deleted in high-growth mice. The size of the deletion estimated from the clone lengths is approximately 500 kb. Using exon trapping, the murine Raidd/Cradd gene was identified in the hg region, and its cDNA sequence and expression pattern were determined. The human RAIDD/CRADD was mapped using radiation hybrid mapping to human chromosome 12, 2.9 cR distal to marker AFMB311WC5. The identified Raidd/Cradd gene, which is deleted in high-growth mice, presents a potential candidate for hg.
Horvat
S
S
Division of Molecular Biology, Roslin Institute (Edinburgh), Roslin, EH25 9PS, Scotland, United Kingdom. HORVATS@BBSRC.AC.UK
Medrano
J F
JF
D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D053420:CRADD Signaling Adaptor Protein; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D020451:Contig Mapping; D018076:DNA, Complementary; D017353:Gene Deletion; D005819:Genetic Markers; D006128:Growth / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016324:Sequence Tagged Sites
D048868:Adaptor Proteins, Signal Transducing; D053420:CRADD Signaling Adaptor Protein; C104252:CRADD protein, human; D002352:Carrier Proteins; C484481:Cradd protein, mouse; D018076:DNA, Complementary; D005819:Genetic Markers; D011506:Proteins