title: NO concentration in the periendothelial area of the femoral artery of the dog measured in vivo.
abstract: .NO concentration was measured in the periendothelial area of the femoral artery by Malinski's porphyrinic .NO sensor in seven anaesthetized dogs. The basal concentration was 154.2 +/- 5.6 nM and two-minute intraarterial infusions of acetylcholine (3-4 micrograms/ml/min) or bradykinin (30-40 ng/ml/min) increased this value significantly to 204.3 +/- 16.4 and 266.5 +/- 16.4 nM (P < 0.01), respectively. Inhibition of .NO synthase by L-NAME (50 mg/kg) declined the basal .NO concentration only to 137.2 +/- 3.3 nM (P < 0.01). Subsequent administration of acetylcholine and bradykinin attenuated significantly the increase in .NO concentration. Surprisingly, both agonists still induced a significant increase of .NO concentration by 125.3 +/- 8.3 and 156.6 +/- 26.9 nM, respectively (P < 0.01). One of the possible explanations may be that besides arginine-citrulline plus the .NO pathway other sources of .NO could be involved in the high level of .NO after .NO synthase blockade by L-NAME.
authors.affiliation: Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Gerová
authors.forename: S
authors.initials: S
authors.lastname: Mesaros
authors.forename: F
authors.initials: F
authors.lastname: Kristek
authors.forename: M
authors.initials: M
authors.lastname: Kittová
authors.forename: T
authors.initials: T
authors.lastname: Malinski
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D015374:Biosensing Techniques; D001920:Bradykinin / Q000494:pharmacology; D004285:Dogs; D004730:Endothelium, Vascular / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005263:Femoral Artery / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D000109:Acetylcholine; D001920:Bradykinin; D019331:NG-Nitroarginine Methyl Ester