[Detection of p53 gene mutation in paraffin-embedded asbestos-related lung cancer tissue].
Paraffin-embedded asbestos-related lung cancer tissue of ten cases was analyzed with polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) to study its genetic mutation of exon 5, 7 and 8 of anti-oncogene p53 by silver stain. Results showed that fragment of exon 7 or 8 of p53 gene in four cases was positive in silver-staining analysis of PCR-SSCP. Exon 7 or 8 fragments of p53 gene was detected positive for mutation in seven of these ten cases with autoradiographic analysis of PCR-SSCP. No exon 5 fragment was found positive for mutation in these ten cases with both methods. Agreement between results of silver staining and those of autoradiographic PCR-SSCP was 60%. Hence, autoradiographic method could be replaced by silver staining, a simpler and more sensitive one, in PCR-SSCP to study gene mutation of asbestosrelated lung cancer.
Fu
D
D
Department of Molecular Biology, Institute of Occupational Medicine, Beijing.
Liu
B
B
Wang
H
H
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D001195:Asbestosis / Q000235:genetics* / Q000473:pathology; D016158:Genes, p53 / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology; D009154:Mutation; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational