title: [Participation of phosphoinositide-specific phospholipase Cgamma1 and STAT1 protein in the formation of latent complexes of signal proteins].
abstract: It is known that the growth factor activates appropriate membrane receptors which become starting points of cascades of protein-protein interactions leading to cellular response. Recent data suggest that different signalling pathways may cross-talk during the cellular response. Here we show that phosphoinositide-specific phospholipase C gamma 1, one of the key elements in phosphoinositide pathway of signal transduction, is physically associated with members of the STAT pathway. The precipitation of phospholipase C gamma 1, using polyclonal antibody in A-431 cells, leads to co-immunoprecipitation of STAT1 alpha and STAT1 beta, as well as STAT3. The formation of such complexes was observed in both unstimulated and EGF stimulated cells. The participation of SH3-domains in the formation of such complexes is discussed.
authors.affiliation: Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
authors.forename: A L
authors.initials: AL
authors.lastname: Evdonin
authors.forename: D B
authors.initials: DB
authors.lastname: Tvorogov
authors.forename: N V
authors.initials: NV
authors.lastname: Tsupkina
authors.forename: N N
authors.initials: NN
authors.lastname: Nikol'skiĭ
authors.forename: N D
authors.initials: ND
authors.lastname: Medvedeva
mesh_terms: D002461:Cell Line, Transformed; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D051966:Phospholipase C gamma; D050794:STAT1 Transcription Factor; D015398:Signal Transduction; D015534:Trans-Activators / Q000378:metabolism*; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D007527:Isoenzymes; D050794:STAT1 Transcription Factor; C494079:STAT1 protein, human; D015534:Trans-Activators; D004815:Epidermal Growth Factor; D010738:Type C Phospholipases; D051966:Phospholipase C gamma