Protective effect of hyperin against myocardial ischemia and reperfusion injury.
OBJECTIVE
To study the protective and antiperoxidative effects of hyperin (hyperoside; quercetin-3-O-galactoside; Hyp) on myocardial ischemia/reperfusion.


METHODS
The rabbit anterior descenging branch of left coronary artery was occluded for 60 min and then released to allow reperfusion for 20 min. Hemodynamics (LVP, LV +/- dp/dt) and electrocardiogram (ECG, lead II) were monitored continuously with polygraph. After reperfusion, the blood sample and myocardium were taken to assay plasma creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and cations in myocardium. Using a Langendorff system, the isolated heart of rat was initiated by ischemia for 40 min followed by 30 min of reperfusion. Malondialdehyde (MDA) contents of cardiac effluent and myocardium were measured with fluorescence spectrophotometer.


RESULTS
Hyp 10 mg.kg-1 i.v. depressed changes in LVP, LV +/- dp/dtmax, ECG, plasma CPK, LDH, and cations (Ca2+, Mg2+, Na+) in myocardium induced by ischemia/reperfusion in rabbits. Hyp 10 and 100 mumol.L-1 markedly reduced the increase in MDA production in isolated rat hearts after ischemia/reperfusion.


CONCLUSIONS
Hyp possesses a protective effect against myocardial ischemia/reperfusion injury via attenuating lipid peroxidation.
Wang
W Q
WQ
Department of Pharmacology, Anhui Medical University, Hefei, China.
Ma
C G
CG
Xu
S Y
SY
D000818:Animals; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D003402:Creatine Kinase / Q000097:blood; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000378:metabolism* / Q000503:physiopathology; D009206:Myocardium / Q000378:metabolism; D011794:Quercetin / Q000031:analogs & derivatives* / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D002121:Calcium Channel Blockers; D008315:Malondialdehyde; C021304:hyperoside; D011794:Quercetin; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase