title: Involvement of histidine permease (Hip1p) in manganese transport in Saccharomyces cerevisiae.
abstract: In a search for components involved in Mn2+ homeostasis in the budding yeast Saccharomyces cerevisiae, we isolated a mutant with modifications in Mn2+ transport. The mutation was found to be located in HIP1, a gene known to encode a high-affinity permease for histidine. The mutation, designated hip1-272, caused a frameshift that resulted in a stop codon at position 816 of the 1812-bp ORF. This mutation led to Mn2+ resistance, whereas the corresponding null mutation did not. Both hip1-272 cells and the null mutant exhibited low tolerance to divalent cations such as Co2+, Ni2+, Zn2+, and Cu2+. The Mn2+ phenotype was not influenced by supplementary histidine in either mutant, whereas the sensitivity to other divalent cations was alleviated by the addition of histidine. The cellular Mn2+ content of the hip1-272 mutant was lower than that of wild type or null mutant, due to increased rates of Mn2+ efflux. We propose that Hiplp is involved in Mn2+ transport, carrying out a function related to Mn2+ export.
authors.affiliation: Department of Molecular BioTechnology, Graduate School of Engineering, Hiroshima University, Higashi-Hiroshima, Japan.
authors.forename: I C
authors.initials: IC
authors.lastname: Farcasanu
authors.forename: M
authors.initials: M
authors.lastname: Mizunuma
authors.forename: D
authors.initials: D
authors.lastname: Hirata
authors.forename: T
authors.initials: T
authors.lastname: Miyakawa
mesh_terms: D018528:ATP-Binding Cassette Transporters; D026923:Amino Acid Transport Systems, Basic; D001426:Bacterial Proteins; D002455:Cell Division / Q000187:drug effects; D016368:Frameshift Mutation / Q000235:genetics; D008040:Genetic Linkage; D015698:Genomic Library; D006639:Histidine / Q000494:pharmacology; D006706:Homeostasis; D017136:Ion Transport; D008345:Manganese / Q000378:metabolism* / Q000494:pharmacology; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA
chemical_list: D018528:ATP-Binding Cassette Transporters; D026923:Amino Acid Transport Systems, Basic; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D008345:Manganese; D006639:Histidine; C043828:histidine permease, Bacteria