title: hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster.
abstract: The role of HoxX in hydrogenase biosynthesis of Alcaligenes eutrophus H16 was re-examined. The previously characterized hoxX deletion mutant HF344 and a newly constructed second hoxX mutant carrying a smaller in-frame deletion were studied. The second mutant was impaired in the activity of both the soluble and the membrane-bound hydrogenase. The two hydrogenase activities were reduced by approximately 50% due to delayed processing of the active-site-containing large subunits, while hydrogenase gene expression was not affected. We conclude that the mutation in mutant HF344 causes polarity resulting in the observed regulatory phenotype of this mutant. The data presented in this report point to an enhancing function of HoxX in the conversion of the soluble hydrogenase and of the membrane-bound hydrogenase large-subunit precursor. Thus, hoxX encodes a member of the Hyp proteins that are required for the formation of active hydrogenase and was accordingly renamed hypX.
authors.affiliation: Institut für Biologie, Humboldt-Universität zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Buhrke
authors.forename: B
authors.initials: B
authors.lastname: Friedrich
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics; D003001:Cloning, Molecular; D020118:Cupriavidus necator / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D017353:Gene Deletion; D005798:Genes, Bacterial / Q000235:genetics*; D006801:Humans; D006864:Hydrogenase / Q000096:biosynthesis / Q000235:genetics*; D015151:Immunoblotting; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics; D009154:Mutation; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000502:physiology
chemical_list: D001426:Bacterial Proteins; C088143:HoxX protein, Alcaligenes eutrophus; D008565:Membrane Proteins; D014157:Transcription Factors; C043296:nickel-iron hydrogenase; D006864:Hydrogenase