title: Comparative classification of Acinetobacter baumannii strains using seven different typing methods.
abstract: A group of 49 Acinetobacter baumannii strains obtained from several hospital outbreaks and some sporadic cases were typed by biotyping, antimicrobial susceptibility testing, pulsed-field gel electrophoresis (PFGE), plasmid typing, multilocus enzyme electrophoresis, whole-cell protein profile, and Fourier-transform infrared (FT-IR) spectroscopy. All these methods have shown a high degree of reproducibility and are capable of recognising strains from the same epidemiological event. However, their power to discriminate between epidemiologically unrelated strains varies, with PFGE being superior to the other methods investigated. FT-IR spectroscopy, which has not yet been used for typing of Acinetobacter strains, proved to be a very rapid and highly reproducible method, but was somewhat limited in its discriminating power.
authors.affiliation: Robert-Koch-Institut, Bereich Wernigerode.
authors.forename: G
authors.initials: G
authors.lastname: Seltmann
authors.forename: W
authors.initials: W
authors.lastname: Beer
authors.forename: H
authors.initials: H
authors.lastname: Claus
authors.forename: H
authors.initials: H
authors.lastname: Seifert
mesh_terms: D000150:Acinetobacter / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D000151:Acinetobacter Infections / Q000382:microbiology; D001426:Bacterial Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D004592:Electrophoresis, Starch Gel; D004798:Enzymes / Q000032:analysis; D006801:Humans; D010957:Plasmids; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004798:Enzymes