Degradation of fluoranthene by Pasteurella sp. IFA and Mycobacterium sp. PYR-1:isolation and identification of metabolites.
The findings from a biodegradability study of fluoranthene using two pure bacterial strains, Pasteurella sp. IFA (B-2) and Mycobacterium sp. PYR-1 (AM) are reported. Of total fluoranthene, 24% (B-2) and 46% (AM) was biodegraded in an aqueous medium during 14 d of incubation at room temperature. During this period the bacteria were capable of mineralizing approximately two-thirds (B-2) and four-fifths (AM) of biodegraded fluoranthene to CO2, while one-third (B-2) and one-fifth (AM) of the original fluoranthene remained as stable metabolic products. These metabolites were isolated using liquid-liquid extraction and identified using gas chromatography-mass spectrometry (GC-MS) and derivatization techniques. Two metabolites (9-fluorenone-1-carboxylic acid and 9-fluorenone) were identified by GC-MS directly, while the metabolites 9-fluorenone-1-carboxylic acid, 9-hydroxyfluorene, 9-hydroxy-1-fluorene-carboxylic acid, 2-carboxybenzaldehyde, benzoic acid and phenylacetic acid were determined in their derivatized forms. From the identified metabolites, a fluoranthene biodegradation pathway was proposed for Pasteurella sp. IFA.
Sepic
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Department of Environmental Sciences, Jozef Stefan Institute, National Institute of Biology, Ljubljana, Slovenia.
Bricelj
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Leskovsek
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D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D002849:Chromatography, Gas; D003470:Culture Media; D005449:Fluorenes / Q000378:metabolism*; D013058:Mass Spectrometry; D009161:Mycobacterium / Q000254:growth & development / Q000378:metabolism*; D010101:Oxygen Consumption; D010325:Pasteurella / Q000254:growth & development / Q000378:metabolism*; D012989:Soil Pollutants / Q000378:metabolism*
D003470:Culture Media; D005449:Fluorenes; D012989:Soil Pollutants; D002245:Carbon Dioxide; C007738:fluoranthene