Detection of HLA-A*24 null alleles by DNA typing methods.
A number of cases have been identified (seven unrelated individuals from the Northern Ireland bone marrow donor registry and two family groups) where an HLA-A*24 allele fails to express the normal HLA-A24 antigen. Family information has revealed common haplotypes with respect to each non-expressed allele indicating that the occurrence of these mutations has been a recent event. Two methods for the clinical typing of these alleles have been evaluated--PCR-SSOP and PCR-SSCP analysis.
Williams
F
F
Northern Ireland Regional Histocompatibility and Immunogenetics Laboratory, City Hospital, Belfast.
Curran
M D
MD
Middleton
D
D
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D005091:Exons; D015234:HLA-A Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007438:Introns; D007494:Ireland / Q000453:epidemiology; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D016415:Sequence Alignment
D015234:HLA-A Antigens; D004247:DNA