[Effect of interleukin-2 on the proliferation of cultured rat anterior pituitary cells].
The present study was to investigate whether IL-2 is involved in the proliferation control of anterior pituitary (AP) cells. Primary culture of AP cells was obtained from either male or female Sprague-Dawley rats. The effects of IL-2 were assessed by 3H-thymidine incorporation rate. It was found that: (1) IL-2 (10-500 U/ml) significantly stimulated 3H-TdR incorporation into AP cells of female pregnant as well as none-pregnant rats, but inhibited 3H-TdR incorporation in the male rats. (2) The stimulatory effect of IL-2 was reversed in overiectomized rats, but could be restored when the overiectomized rats were treated with estradiol benzoate (5 micrograms/d per rat). (3) When the male rats were orchiectomized for 2 weeks, the inhibitory effects of IL-2 did not occur. The above data indicated that the proliferative effect of IL-2 on AP is a joint action of sex steroids hormones.
Wang
G F
GF
Fourth Military Medical University, Xian.
Zhu
Y L
YL
Hu
Y Z
YZ
Zhang
W H
WH
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D007376:Interleukin-2 / Q000494:pharmacology*; D008297:Male; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D007376:Interleukin-2; D004958:Estradiol