Application of conductance techniques to the detection of thermophilic Campylobacter spp. in river water.
A study of basal medal identified Campylobacter enrichment broth, with (CEB+) and without (CEB) antibiotic supplement, as a suitable medium for the detection and enumeration of Campylobacter jejuni, C. coli and C. lari within aqueous samples via conductance methodology. Despite apparent differences in conductivity profiles between species in the presence of antibiotics, no significant differences (P < 0.05) were detected between detection times for each species tested. CEB+ was successfully employed within a combined enrichment and conductance protocol to the detection of C. jejuni from river water at a concentration of 1 CFU ml-1 from 83% of samples in under 39 h and thus demonstrated an improvement over an applied conventional membrane filtration technique.
Thomas
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Microbiology Department, School of Applied Sciences, University of Wolverhampton, UK. Chris.Thomas@ul.ie
Hill
D J
DJ
Mabey
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D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002167:Campylobacter / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D003470:Culture Media; D004553:Electric Conductivity; D005618:Fresh Water / Q000382:microbiology*; D008851:Micropore Filters; D014871:Water Microbiology; D014876:Water Pollution
D000900:Anti-Bacterial Agents; D003470:Culture Media