title: Distribution of alpha3, alpha5 and alpha(v) integrin subunits in mature and immature human oocytes.
abstract: The distribution of three integrin subunits, alpha3, alpha5 and alpha(v), in immature and mature human oocytes has been examined using immunofluorescence and confocal microscopy. The results demonstrate that both alpha5 and alpha(v) are present at the germinal vesicle stage, while alpha3 was only detected in oocytes after germinal vesicle breakdown, in metaphase I and II stage oocytes. The cortical concentration of integrin subunits alpha3 and alpha5 is consistent with their localization in the oolemma. In contrast, the homogeneous distribution of alpha(v) throughout the oocyte suggests the existence of cytoplasmic reservoirs of this protein in the oocyte.
authors.affiliation: Assisted Conception Unit, King's College School of Medicine and Dentistry, London, UK.
authors.forename: G
authors.initials: G
authors.lastname: Capmany
authors.forename: M
authors.initials: M
authors.lastname: Mart
authors.forename: J
authors.initials: J
authors.lastname: Santaló
authors.forename: V N
authors.initials: VN
authors.lastname: Bolton
mesh_terms: D015703:Antigens, CD / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D007211:Indoles / Q000737:chemistry; D039422:Integrin alpha3; D039482:Integrin alpha5; D039564:Integrin alphaV; D016023:Integrins / Q000276:immunology / Q000378:metabolism*; D008677:Metaphase; D009865:Oocytes / Q000502:physiology*
chemical_list: D015703:Antigens, CD; D007211:Indoles; D039422:Integrin alpha3; D039482:Integrin alpha5; D039564:Integrin alphaV; D016023:Integrins; C007293:DAPI