title: Cultured, autologous nucleus pulposus cells induce functional changes in spinal nerve roots.
abstract: METHODS
  Nerve conduction velocity in pig nerve roots was assessed after application of various preparations of nucleus pulposus and control.
  
  
  OBJECTIVE
  To study whether cultured nucleus pulposus cells could reduce nerve conduction velocity after epidural application.
  
  
  BACKGROUND
  It is known that nucleus pulposus applied epidurally may reduce the nerve conduction velocity of the adjacent nerve roots and that this reduction seems to be related to the cells of the nucleus pulposus.
  
  
  METHODS
  Nucleus pulposus cells and fibroblasts were cultured for 3 weeks, and various preparations were applied to the cauda equina in 29 pigs. The cells were always from the same animals from which they had been harvested. After 1 week, nerve conduction velocity was determined by local electrical stimulation.
  
  
  RESULTS
  Application of live fibroblasts and conditioned culture medium from the nucleus pulposus cell culture dishes did not induce significant reduction of conduction velocity, compared with application of dead fibroblasts, which served as control. However, application of live and dead nucleus pulposus cells induced significant reductions.
  
  
  CONCLUSIONS
  Application of nucleus pulposus cells reproduced the previously seen reduction in nerve conduction velocity induced by nonmodified nucleus pulposus. Because membranes of the nucleus pulposus cells had similar effects, it can be assumed that the effects are related to membrane-bound substances or structures.
authors.affiliation: Department of Orthopaedics, Göteborg University, Sahlgrenska University Hospital, Gothenburg, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Kayama
authors.forename: K
authors.initials: K
authors.lastname: Olmarker
authors.forename: K
authors.initials: K
authors.lastname: Larsson
authors.forename: E
authors.initials: E
authors.lastname: Sjögren-Jansson
authors.forename: A
authors.initials: A
authors.lastname: Lindahl
authors.forename: B
authors.initials: B
authors.lastname: Rydevik
mesh_terms: D000818:Animals; D002420:Cauda Equina / Q000502:physiology*; D002450:Cell Communication / Q000502:physiology; D017690:Cell Transplantation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D007403:Intervertebral Disc / Q000166:cytology* / Q000637:transplantation*; D007405:Intervertebral Disc Displacement / Q000473:pathology; D017116:Low Back Pain / Q000473:pathology; D009431:Neural Conduction / Q000502:physiology*; D013552:Swine