title: Antigen heterogeneity among Mycoplasma mycoides subsp. mycoides SC isolates: discrimination of major surface proteins.
abstract: The protein and antigen profiles of 60 isolates, strains and the type strain PG1 of Mycoplasma mycoides subsp. mycoides s.c. were compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis and immunoblot analysis. Analysis using contagious bovine pleuropneumonia antisera and hyperimmune rabbit sera against several representative strains revealed some differences in protein profiles and variability in antigens among strains from different geographic regions. The most common antigenic bands had the molecular masses of 110, 95, 80, 69, 62, 60, 48, 44, 39 and 38 kDa. There were differences among European strains, where a larger group coming from Italy lacked the p98 antigen, thus, with one exception, distinguishing the Italian strains from Portuguese, French and Spanish strains. African, Australian and PG1 strains showed heterogenic profiles, with quantitative differences and in a few strains some antigenic bands were absent. The group constituting African, Australian and PG1 strains was characterised by the presence of 71.5/70 kDa antigens, which were not detected in European strains. Mycoplasma mycoides subsp. mycoides s.c. membrane proteins were characterised by Triton X-114 partitioning and p110, p98, p95, p62/60 and p48 were identified as immunogenic antigens. The simultaneous presence of these five antigens was common to all the sera examined and, therefore, indicates the diagnostic potential of immunoblotting. Most immunodominant antigens are surface-exposed proteins as determined by the trypsin treatment.
authors.affiliation: Laboratório Nacional de Investigação Veterinária, Lisboa, Portugal.
authors.forename: R
authors.initials: R
authors.lastname: Gonçalves
authors.forename: J
authors.initials: J
authors.lastname: Regalla
authors.forename: J
authors.initials: J
authors.lastname: Nicolet
authors.forename: J
authors.initials: J
authors.lastname: Frey
authors.forename: R
authors.initials: R
authors.lastname: Nicholas
authors.forename: J
authors.initials: J
authors.lastname: Bashiruddin
authors.forename: P
authors.initials: P
authors.lastname: de Santis
authors.forename: A P
authors.initials: AP
authors.lastname: Gonçalves
mesh_terms: D000349:Africa; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis; D001315:Australia; D001426:Bacterial Proteins / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000382:microbiology; D005060:Europe; D005843:Geography; D015511:Goat Diseases / Q000175:diagnosis / Q000382:microbiology; D006041:Goats; D007106:Immune Sera; D009176:Mycoplasma mycoides / Q000145:classification* / Q000276:immunology* / Q000302:isolation & purification; D011002:Pleuropneumonia, Contagious / Q000175:diagnosis* / Q000382:microbiology; D011817:Rabbits; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology
chemical_list: D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D007106:Immune Sera