Up-regulation of thioredoxin expression in motor neurons after nerve injury.
A substantial up-regulation of thioredoxin, a dithiol/disulfide oxido-reductase, in adult rat motoneurons following hypoglossal nerve axotomy, was demonstrated by using both in situ hybridization and immunohistochemistry. Although thioredoxin is normally accumulated more in the nucleus of a motoneuron rather than in the cytoplasm, a dramatic increase of thioredoxin in the cytoplasmic region after nerve injury was observed. The up-regulation of mRNA lasted more than 9 weeks, whereas, the detectable up-regulation of protein was observed for more than 5 weeks.
Mansur
K
K
Department of Anatomy, Asahikawa Medical College, 4-5-3-11 Nishikagura, Asahikawa 078-8510, Japan.
Iwahashi
Y
Y
Kiryu-Seo
S
S
Su
Q
Q
Namikawa
K
K
Yodoi
J
J
Kiyama
H
H
D000818:Animals; D019771:Axotomy; D002467:Cell Nucleus / Q000737:chemistry / Q000473:pathology; D003593:Cytoplasm / Q000737:chemistry; D005786:Gene Expression Regulation / Q000502:physiology; D007002:Hypoglossal Nerve / Q000378:metabolism; D061228:Hypoglossal Nerve Injuries; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009046:Motor Neurons / Q000378:metabolism*; D009457:Neuroglia / Q000737:chemistry / Q000473:pathology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D013879:Thioredoxins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology*
D012333:RNA, Messenger; D013879:Thioredoxins