title: Genetic analysis of inflammatory bowel disease in a large European cohort supports linkage to chromosomes 12 and 16.
abstract: OBJECTIVE
  Inflammatory bowel disease (IBD) is a complex disorder of unknown etiology. Epidemiological investigations suggest a genetic basis for IBD. Recent genetic studies have identified several IBD linkages. The significance of these linkages will be determined by studies in large patient collections. The aim of this study was to replicate IBD linkages on chromosomes 12 and 16 in a large European cohort.
  
  
  METHODS
  Three hundred fifty-nine affected sibling pairs from 274 kindreds were genotyped using microsatellite markers spanning chromosomes 12 and 16. Affection status of the sibling pairs was defined as Crohn's disease (CD) or ulcerative colitis (UC).
  
  
  RESULTS
  Nonparametric statistical analyses showed linkage for both chromosomes. Two-point results for chromosome 12 peaked at D12S303 (logarithm of odds [LOD], 2.15; P = 0.003) for CD and at D12S75 (LOD, 0.92; P = 0.03) for UC. Multipoint analyses produced a peak LOD of 1.8 for CD. Chromosome 16 showed linkage for CD at marker D16S415 (LOD, 1.52; P = 0.007). Multipoint support peaked above markers D16S409 and D16S411 (LOD, 1.7).
  
  
  CONCLUSIONS
  These data are consistent with linkage of IBD to chromosomes 12 and 16. The replication of genetic risk loci in a large independent family collection indicates important and common susceptibility genes in these regions and will facilitate identification of genes involved in IBD.
authors.affiliation: AxyS Pharmaceuticals Inc., La Jolla, California, USA. mark_curran@sequana.com
authors.forename: M E
authors.initials: ME
authors.lastname: Curran
authors.forename: K F
authors.initials: KF
authors.lastname: Lau
authors.forename: J
authors.initials: J
authors.lastname: Hampe
authors.forename: S
authors.initials: S
authors.lastname: Schreiber
authors.forename: S
authors.initials: S
authors.lastname: Bridger
authors.forename: A J
authors.initials: AJ
authors.lastname: Macpherson
authors.forename: L R
authors.initials: LR
authors.lastname: Cardon
authors.forename: H
authors.initials: H
authors.lastname: Sakul
authors.forename: T J
authors.initials: TJ
authors.lastname: Harris
authors.forename: P
authors.initials: P
authors.lastname: Stokkers
authors.forename: S J
authors.initials: SJ
authors.lastname: Van Deventer
authors.forename: M
authors.initials: M
authors.lastname: Mirza
authors.forename: A
authors.initials: A
authors.lastname: Raedler
authors.forename: W
authors.initials: W
authors.lastname: Kruis
authors.forename: U
authors.initials: U
authors.lastname: Meckler
authors.forename: D
authors.initials: D
authors.lastname: Theuer
authors.forename: T
authors.initials: T
authors.lastname: Herrmann
authors.forename: P
authors.initials: P
authors.lastname: Gionchetti
authors.forename: J
authors.initials: J
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Mathew
authors.forename: J
authors.initials: J
authors.lastname: Lennard-Jones
mesh_terms: D002881:Chromosomes, Human, Pair 12 / Q000235:genetics*; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D015331:Cohort Studies; D005060:Europe; D005838:Genotype; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D008126:Lod Score; D018895:Microsatellite Repeats / Q000235:genetics