title: Metabolic regulation of endogenous adenosine release from single neurons.
abstract: The mechanisms that regulate adenosine release in the brain are not well understood. The present study investigated the hypothesis that individual neurons can generate and release sufficient adenosine to regulate their synaptic inputs. We utilized the whole-cell recording technique to apply enzyme inhibitors and nucleotides directly into the cytoplasm of single rat hippocampal CA1 pyramidal neurons. Cytoplasmic delivery of adenosine induced the release of sufficient adenosine to inhibit excitatory synaptic inputs. However, intracellular delivery of nucleotides and enzyme inhibitors failed to increase adenosine receptor-mediated inhibition. These data suggest that while pyramidal neurons in the hippocampus are capable of releasing large amounts of adenosine into the extracellular space, they do not readily form adenosine from endogenous sources.
authors.affiliation: Department of Pharmacology, University of Colorado Health Science Center, Denver 80262, USA.
authors.forename: J M
authors.initials: JM
authors.lastname: Brundege
authors.forename: T V
authors.initials: TV
authors.lastname: Dunwiddie
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D000241:Adenosine / Q000378:metabolism* / Q000502:physiology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D004333:Drug Administration Routes; D004558:Electric Stimulation; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D066298:In Vitro Techniques; D008954:Models, Biological; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018408:Patch-Clamp Techniques; D058915:Purinergic P1 Receptor Antagonists; D017966:Pyramidal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D014372:Tubercidin / Q000031:analogs & derivatives / Q000494:pharmacology
chemical_list: D015226:Dinucleoside Phosphates; D058915:Purinergic P1 Receptor Antagonists; C034214:5-iodotubercidin; D000249:Adenosine Monophosphate; C011440:diadenosine triphosphate; C010192:9-(2-hydroxy-3-nonyl)adenine; D000244:Adenosine Diphosphate; D013806:Theophylline; D000225:Adenine; D000241:Adenosine; D014372:Tubercidin