Respiratory syncytial virus induces the expression of 5-lipoxygenase and endothelin-1 in bronchial epithelial cells.
Respiratory syncytial virus (RSV) infection causes and exacerbates asthma, yet the mechanism by which RSV triggers asthma is poorly understood. Herein, an in vitro model of RSV infection was established using HEp-2 and BEAS-2B bronchial epithelial cell lines, and the expression of 5-lipoxygenase (5-LO), and endothelin-1 (ET-1) was examined. RSV infection increased the expression of 5-LO mRNA and protein in both cell lines, as detected by RT-PCR and western blot analysis, respectively. The levels of leukotrienes also increased in the supernatants of RSV infected cells. Furthermore, RSV infection increased the expression of ET-1 mRNA and protein following RSV infection in a time-dependent manner. It is concluded that RSV infection upregulates the expression of ET-1 and 5-LO in the epithelial cells leading to the production of leukotrienes, which may mediate the consequent exacerbation of asthma.
Behera
A K
AK
Department of Internal Medicine, University of South Florida College of Medicine, and James A. Haley VA Hospital, Tampa, FL 33612, USA.
Kumar
M
M
Matsuse
H
H
Lockey
R F
RF
Mohapatra
S S
SS
D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis*; D016718:Arachidonic Acid / Q000378:metabolism; D001249:Asthma; D001980:Bronchi / Q000166:cytology / Q000821:virology*; D002460:Cell Line; D019332:Endothelin-1 / Q000096:biosynthesis*; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology; D006801:Humans; D015289:Leukotrienes / Q000032:analysis; D012333:RNA, Messenger / Q000096:biosynthesis; D018357:Respiratory Syncytial Virus Infections; D012136:Respiratory Syncytial Viruses / Q000254:growth & development*; D015854:Up-Regulation
D019332:Endothelin-1; D015289:Leukotrienes; D012333:RNA, Messenger; D016718:Arachidonic Acid; D001094:Arachidonate 5-Lipoxygenase