title: New reference reagent for the quality assurance of anti-D antibody detection.
abstract: A UK BTS-NIBSC freeze-dried anti-D preparation has been prepared and used to monitor the performance of routine antibody detection tests and of the test operators. With the day-to-day use of this preparation, adverse changes in test performance and in test operator may be detected and appropriate action taken before the effect becomes significant. Two dilutions of this preparation have been defined, one which should be detected unequivocally in every test; the other, more dilute, may not be detected in every test but is used to monitor changes in performance. Experience with the use of this preparation is reported from three test centres undertaking routine antibody detection tests. By monitoring results over a series of working days, significant differences were noted in operator performance within one test centre, as was a reduced sensitivity of a given test system within one test centre compared with the same system in the other test centres. These differences were detected only by monitoring the results obtained with this preparation.
authors.affiliation: National Institute for Biological Standards and Control (NIBSC), Potters Bar, UK.
authors.forename: P
authors.initials: P
authors.lastname: Phillips
authors.forename: D
authors.initials: D
authors.lastname: Voak
authors.forename: M
authors.initials: M
authors.lastname: Downie
authors.forename: N
authors.initials: N
authors.lastname: Clark
authors.forename: J
authors.initials: J
authors.lastname: Miguta
authors.forename: J
authors.initials: J
authors.lastname: Rawlings
authors.forename: B
authors.initials: B
authors.lastname: Patel
authors.forename: M
authors.initials: M
authors.lastname: Redman
mesh_terms: D003298:Coombs Test / Q000592:standards; D005612:Freeze Drying; D006801:Humans; D007202:Indicators and Reagents / Q000592:standards; D007518:Isoantibodies / Q000097:blood*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012204:Rh-Hr Blood-Group System / Q000276:immunology; D018029:Rho(D) Immune Globulin; D012680:Sensitivity and Specificity; D013647:Task Performance and Analysis
chemical_list: D007202:Indicators and Reagents; D007518:Isoantibodies; C061961:RHO(D) antibody; D012204:Rh-Hr Blood-Group System; D018029:Rho(D) Immune Globulin