Cloning and characterization of human Sep1 (hSEP1) gene and cytoplasmic localization of its product.
We isolated and sequenced a human cDNA (designated as hSEP1) encoding both a homologue of mouse Dhm2 and budding yeast SEP1. The gene was shown to be located on the long arm of chromosome 3 (3q25-26.1). The putative hSEP1 product (hSEP1p) consisted of 1694 amino acid residues with a molecular mass of about 190 kDa. Northern blot analysis showed a major 10-kb mRNA expressed ubiquitously in various organs as well as a minor 5.5-kb mRNA expressed relatively highly in the testis and placenta. hSEP1p is localized in the cytoplasm as examined by cytochemical and Western blot analyses of fractionated cellular extracts. The biological function of hSEP1p was discussed in correlation with its cytoplasmic localization.
Sato
Y
Y
AGENE Research Institute, Kamakura, Kanagawa, Japan.
Shimamoto
A
A
Shobuike
T
T
Sugimoto
M
M
Ikeda
H
H
Kuroda
S
S
Furuichi
Y
Y
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D002893:Chromosomes, Human, Pair 3; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism*; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D005095:Exoribonucleases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029701:Saccharomyces cerevisiae Proteins; D005095:Exoribonucleases; C068398:XRN1 protein, S cerevisiae