title: Effect of pamidronate on bone blood flow in oophorectomized rats.
abstract: In connection with the known inhibitive action of bisphosphonates on bone resorption we were interested in their possible influence on bone blood flow (BBF). We determined BBF (85Sr-microsphere uptake in the tibia, distal femur and diaphysis of femur), cardiac output, density and ash weight of the tibia, as well as 24-h incorporation of 45Ca and 3H-proline into the tibia. Pamidronate (Aredia, CIBA-Geigy, Switzerland) was administered to sham-operated or oophorectomized (OOX) female rats in doses of 0.6 mg i.p. 2 days a week for 4 weeks. 85Sr-microsphere uptake was increased after OOX in the tibia and distal femur, simultaneous pamidronate administration significantly suppressed this increase below the control level. In addition, pamidronate inhibited the 24-h incorporation of 45Ca and 3H-proline in sham-operated females and suppressed the incorporation of 3H-proline that was increased after OOX. Bone density and ash weight were significantly increased after pamidronate administration in both sham-operated and OOX rats. The results of both experiments showing a significant effect of pamidronate on BBF and incorporation of 45Ca and 3H-proline require further verification and elucidation.
authors.affiliation: Laboratory for Endocrinology and Metabolism, First Faculty of Medicine, Charles University, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Kapitola
authors.forename: J
authors.initials: J
authors.lastname: Zák
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity / Q000187:drug effects; D001842:Bone and Bones / Q000098:blood supply*; D002132:Calcium Radioisotopes / Q000378:metabolism; D004164:Diphosphonates / Q000494:pharmacology*; D005260:Female; D005269:Femur / Q000098:blood supply; D008863:Microspheres; D010052:Ovariectomy; D000077268:Pamidronate; D011392:Proline / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013326:Strontium Radioisotopes; D013977:Tibia / Q000098:blood supply; D014316:Tritium
chemical_list: D002132:Calcium Radioisotopes; D004164:Diphosphonates; D013326:Strontium Radioisotopes; D014316:Tritium; D011392:Proline; D000077268:Pamidronate