Effects of captopril and enalaprilat on intracellular Ca2+ content in isolated cardiomyocytes from rats.
OBJECTIVE
To study the effects of ACEI captopril (Cap) and enalaprilat (Ena) on intracellular Ca2+ concentration ([Ca2+]i) in cardiac myocytes isolated from SHR and WKY rats.


METHODS
Using fluorescent probe Fura 2-AM combined with computer image processing technique to measure [Ca2+]i.


RESULTS
Resting [Ca2+]i was higher in SHR cardiac myocytes (174 +/- 5 nmol.L-1) than that in WKY rat myocytes (148 +/- 15 nmol.L-1, P < 0.01). Cap and Ena decreased the resting [Ca2+]i in SHR myocytes (161 +/- 11 and 166 +/- 7 nmol.L-1, respectively, P < 0.05) but not in WKY rat myocytes (P > 0.05). Both drugs inhibited [Ca2+]i increment induced by KCI, NE, or Ang II in SHR and WKY rat myocytes except on KCI-induced [Ca2+]i increment in WKY rat myocytes (P > 0.05).


CONCLUSIONS
Cap and Ena had direct effects on pathological voltage-operated calcium channel in cardiac myocytes.
Wang
J
J
Department of Pharmacology, Shanghai Second Medical University, China.
Zhang
L
L
Qi
J H
JH
Zhang
P J
PJ
Wei
P J
PJ
Gu
P K
PK
Jin
Z J
ZJ
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000187:drug effects; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D015773:Enalaprilat / Q000494:pharmacology*; D006973:Hypertension / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D000806:Angiotensin-Converting Enzyme Inhibitors; D015220:Calcium Channels; D002216:Captopril; D015773:Enalaprilat; D002118:Calcium