Characterization of the lys2 gene of Penicillium chrysogenum encoding alpha-aminoadipic acid reductase.
A DNA fragment containing a gene homologous to LYS2 gene of Saccharomyces cerevisiae was cloned from a genomic DNA library of Penicillium chrysogenum AS-P-78. It encodes a protein of 1409 amino acids (Mr 154859) with strong similarity to the S. cerevisiae (49.9% identity) Schizosaccharomyces pombe (51.3% identity) and Candida albicans (48.12% identity) alpha-aminoadipate reductases and a lesser degree of identity to the amino acid-activating domains of the non-ribosomal peptide synthetases, including the alpha-aminoadipate-activating domain of the alpha-aminoadipyl-cysteinyl-valine synthetase of P. chrysogenum (12.4% identical amino acids). The lys2 gene contained one intron in the 5'-region and other in the 3'-region, as shown by comparing the nucleotide sequences of the cDNA and genomic DNA, and was transcribed as a 4.7-kb monocistronic mRNA. The lys2 gene was localized on chromosome III (7.5 Mb) in P. chrysogenum AS-P-78 and on chromosome IV (5.6 Mb) in strain P2, whereas the penicillin gene cluster is known to be located in chromosome I in both strains. The lys2-encoded protein is a member of the aminoacyladenylate-forming enzyme family with a reductase domain in its C-terminal region.
Casqueiro
J
J
Area of Microbiology, Faculty of Biology, University of León, Institute of Biotechnology INBIOTEC Parque Científico de León, Spain.
Gutiérrez
S
S
Bañuelos
O
O
Fierro
F
F
Velasco
J
J
Martín
J F
JF
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D003066:Coenzyme A Ligases; D016384:Consensus Sequence; D016521:Electrophoresis, Gel, Pulsed-Field; D015698:Genomic Library; D007438:Introns; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D008239:Lysine; D008969:Molecular Sequence Data; D010408:Penicillium chrysogenum / Q000201:enzymology / Q000235:genetics*; D010453:Peptide Synthases; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D015183:Restriction Mapping; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014443:Tyrosine; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014443:Tyrosine; D000445:Aldehyde Oxidoreductases; D050647:L-Aminoadipate-Semialdehyde Dehydrogenase; D003066:Coenzyme A Ligases; C090799:FAA2 protein, S cerevisiae; D000098859:Long-Chain-Fatty-Acid-CoA Ligase; D010453:Peptide Synthases; D008239:Lysine