Purification and characterization of 38-kDa protein in plasma of dietary magnesium-deficient rat.
We have found an increase of a 38-kDa plasma protein level from 10 to 30 days in rats on a magnesium-deficient diet (Mg; 0.001 per cent), using sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This 38-kDa protein returned to the control level after feeding the rats a magnesium-sufficient diet (Mg; 0.07 per cent) for 1 week. Purification of the protein by reverse-phase HPLC separated it into two components (p38k-1 and p38k-2). Amino acid analysis showed that these components were both haptoglobin (Hp) beta-chain. Furthermore, hemoglobin-immobilized affinity chromatography analysis confirmed the increase of Hp in magnesium-deficient rat plasma. This is the first demonstration of an increase of Hp in plasma of dietary magnesium-deficient rats.
Nakagawa
M
M
Department of Veterinary Pharmacology, Faculty of Agriculture, Kagoshima University, Japan.
Matsuo
T
T
Fujimoto
S
S
Nishio
A
A
D000595:Amino Acid Sequence; D000818:Animals; D001798:Blood Proteins / Q000032:analysis* / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006242:Haptoglobins / Q000032:analysis; D008274:Magnesium / Q000097:blood; D008275:Magnesium Deficiency / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D051381:Rats; D017208:Rats, Wistar
D001798:Blood Proteins; D006242:Haptoglobins; D008274:Magnesium