Cardiovascular effects of total soyasaponin in central nervous system and its relationship with monoamine transmitters.
OBJECTIVE
To study the cardiovascular effect of total soyabeans saponins (TS) in brain and its relationship with monoamines.


METHODS
After injection of TS (75 micrograms) into ventriculus lateralis cerebri (VLC) the changes of blood pressure (BP) and heart rate (HR) were observed and the contents of monoamines both in peripheral blood and brain (telencephalon, diencephalon, brainstem) were measured respectively by HPLC-ECD and fluorophotometry.


RESULTS
After injection of TS into VLC, BP rise from 11.59 +/- 0.84 to 14.59 +/- 0.69 kPa; HR increased from 411 +/- 21 to 465 +/- 14 bpm; the contents of NE and E in peripheral blood increased from 6 +/- 3 to 64 +/- 44, from 6 +/- 2 to 38 +/- 34 nmol/L plasma, respectively, NE in brainstem increased from 14 +/- 0 to 18 +/- 3 nmol/g wet tissue respectively, but the contents of 5-HT in the 3 areas measured in the experiment decreased: in telecephalon from 9 +/- 1 to 5 +/- 1, in diencephalon from 14 +/- 2 to 7 +/- 2, in brainstem from 14 +/- 3 to 6 +/- 1 nmol/g wet tissue.


CONCLUSIONS
The cardiovascular effects of TS in CNS were involved in the monoamine transmitters.
Guo
J H
JH
Department of Biochemistry, Norman Bethune University of Medical Sciences, Changchun, China.
Tang
Y H
YH
Wei
Y D
YD
Li
J H
JH
Zhao
H
H
Wang
S
S
D000818:Animals; D015306:Biogenic Monoamines / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects*; D001921:Brain / Q000378:metabolism; D006339:Heart Rate / Q000187:drug effects*; D007276:Injections, Intraventricular; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*
D015306:Biogenic Monoamines; D012503:Saponins