title: [Effects of serotonin on the proliferation of pulmonary arterial smooth muscle cell under hypoxia].
abstract: The effects of hypoxia on the bovine pulmonary arterial smooth muscle cell (PASM) proliferation and serotonin transporter gene expression were investigated using 3H-Thymidine incorporation, immunocytochemical staining and mRNA dot blot hybridyzation, the results were as follows, PASM 3H-Thymidine incorporation increased in response to anoxia for 24 h (P < 0.05). The anoxia-induced increased in DNA synthesis of PASM was significantly stimulated by 5-HT (100, 10 and 1 mumol/L) (P < 0.001), but 5-HT did not increase 3H-TdR incorporation under normoxic condition. Immunocytochemical reaction of serotonin in PASM under normoxic condition was markedly stronger than that of anoxic PASM (P < 0.001). Dot blot analysis showed hypoxia increased serotonin transporter mRNA expression of PASM (P < 0.01). These results indicate that stimulation of PASM proliferation with enhanced serotonin transporter gene expression and high consumption of 5-HT in hypoxia or/and anoxia may take part in the development of hypoxic pulmonany hypertention.
authors.affiliation: Department of Pathophysiology, Third Military Medical College, Chongqing.
authors.forename: J
authors.initials: J
authors.lastname: Liu
authors.forename: P Y
authors.initials: PY
authors.lastname: Wang
authors.forename: D C
authors.initials: DC
authors.lastname: Luo
authors.forename: Z H
authors.initials: ZH
authors.lastname: Yu
authors.forename: B Y
authors.initials: BY
authors.lastname: Sun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006976:Hypertension, Pulmonary / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D011651:Pulmonary Artery / Q000166:cytology; D012701:Serotonin / Q000494:pharmacology*
chemical_list: D012701:Serotonin