title: Successful experimental multiorgan transplant from non-heart-beating donors using percutaneous cardiopulmonary support.
abstract: Organ procurement from non-heart-beating donors (NHBDs) may expand donor pools. In this study, the method of reanimation of heart, lung, and kidney in NHBDs by percutaneous cardiopulmonary support (PCPS) was evaluated. Thirteen beagles were asphyxiated after being given prostacyclin analogue, verapamil, propranolol, and nafmostat mesilate intravenously. Thirty minutes after cardiac arrest, the body was reperfused by PCPS for 1 hr. PCPS priming fluid contained the four drugs above and KCl. Eight hearts immersed in University of Wisconsin (Madison, WI) solution for 24 hr were transplanted orthotopically using leukocyte depleted blood cardioplegia, five left lungs immersed in modified Collins solution were transplanted orthotopically, and five kidneys immersed in University of Wisconsin solution were transplanted heterotopically. All donor hearts arrested without ventricular fibrillation. All transplanted hearts beat spontaneously, and all animals were weaned from cardiopulmonary bypass without inotropic support. The oxygen and carbon dioxide pressure of pulmonary vein blood in the donor lung were no different from those in the recipient lung. All transplanted kidneys made urine soon after reperfusion. These data suggested that hearts, lungs, and kidneys from NHBDs pretreated with four drugs and reanimated with PCPS can be transplanted successfully and that this method may expand the donor pool.
authors.affiliation: Department of Surgery, Osaka University Medical School, Suita, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Fukushima
authors.forename: R
authors.initials: R
authors.lastname: Shirakura
authors.forename: J C
authors.initials: JC
authors.lastname: Chang
authors.forename: H
authors.initials: H
authors.lastname: Izutani
authors.forename: M
authors.initials: M
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: M
authors.initials: M
authors.lastname: Yoshitatsu
authors.forename: I
authors.initials: I
authors.lastname: Ahamet
authors.forename: S
authors.initials: S
authors.lastname: Saito
authors.forename: H
authors.initials: H
authors.lastname: Matsuda
mesh_terms: D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D004285:Dogs; D005260:Female; D005978:Glutathione; D016027:Heart Transplantation; D007328:Insulin; D016030:Kidney Transplantation; D016040:Lung Transplantation; D008297:Male; D019188:Organ Preservation Solutions; D011887:Raffinose; D009927:Tissue and Organ Procurement
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose