Flow cytometric light scattering analysis, acrosome reaction, reactive oxygen species production and leukocyte contamination of semen preparation in prediction of fertilization rate in vitro.
The effects on fertilization of the morphology of spermatozoa, acrosome reaction, reactive oxygen species (ROS) production, leukocyte contamination and the light scattering in flow cytometry of semen preparation were investigated in 73 couples undergoing their first in-vitro fertilization treatment. All men had normal concentrations of spermatozoa with sufficient motility (> or = 50%) and yield (> or = 6 x 10(6)) in the semen preparation on the day of oocyte retrieval. The light scattering properties of all cells present in the semen preparation, as assessed with flow cytometry, were correlated with fertilization. The proportion of metaphase I oocytes was also correlated with the fertilization rate of all collected oocytes and of metaphase II oocytes. ROS production, leukocyte contamination, spontaneous or calcium ionophore-stimulated acrosome reaction, percentage of normal morphology, and multiple anomalies index had no independent contribution.
Moilanen
J M
JM
Infertility Clinic, The Family Federation of Finland, Helsinki.
Carpén
O
O
Hovatta
O
O
D020101:Acrosome Reaction; D000328:Adult; D005260:Female; D005307:Fertilization in Vitro; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D008297:Male; D011237:Predictive Value of Tests; D018873:Pregnancy Rate; D017382:Reactive Oxygen Species; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*
D017382:Reactive Oxygen Species