The use of phosphocreatine plus ADP as energy source for motility of membrane-deprived trout spermatozoa.
Live trout spermatozoa initiate flagellar motility for a short period of time (30 s at 18 degrees C), during which their mean beat frequency (BF) decreases steadily from 60 to 20 Hz; motility then stops abruptly. When demembranated, the motility of axonemes lasts much longer, up to 20 min, with high beat frequency, provided that ATP (millimolar concentration) and cAMP (micromolar) are added. In the present paper, the motility of demembranated trout sperm was investigated in the absence of added ATP in various incubation conditions relative to other substrates. Without the addition of exogenous creatine kinase, the addition of phosphocreatine (PCr) and ADP shows the appearance of a progressive activation of all sperm models with BF increasing with time up to high values. Without the addition of cAMP, the BF increases to lower values but flagella propagated poorly coordinated waves for only a few min. Similar progressive activation is also observed when only ADP is added (without any previous in vivo activation) and BF increases up to moderate values. In this latter case, no activation occurs without addition of cAMP. The respective roles of creatine kinase and adenylate kinase in this process were investigated by addition of specific inhibitors such as fluorodinitrobenzene and P1,P5-di(adenosine-5')pentaphosphate in the above described conditions. We conclude from these observations that all the elements necessary for a coupling between ADP/PCr/creatine kinase on one hand and ATP/ADP/dynein on the other appear to be present in trout spermatozoa: thus the existence of a shuttle sustaining this coupling is strongly suggested.
Saudrais
C
C
Laboratoire de Physiologie des Poissons, INRA, Campus de Beaulieu, Rennes, France.
Fierville
F
F
Loir
M
M
Le Rumeur
E
E
Cibert
C
C
Cosson
J
J
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D003402:Creatine Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D015226:Dinucleoside Phosphates / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018715:Microscopy, Video; D008954:Models, Biological; D017686:Oncorhynchus mykiss / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism*; D013081:Sperm Motility / Q000502:physiology*; D013082:Sperm Tail / Q000378:metabolism; D013094:Spermatozoa / Q000201:enzymology / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
D015226:Dinucleoside Phosphates; D010725:Phosphocreatine; D014638:Vanadates; C012275:P(1),P(5)-di(adenosine-5'-)pentaphosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D004139:Dinitrofluorobenzene; D000242:Cyclic AMP; D003402:Creatine Kinase; D000263:Adenylate Kinase; D014357:Trypsin