Genetic instability of microsatellite sequences in non-small cell lung cancers.
To evaluate the frequency and pattern of microsatellite instability in NSCLCs, we examined 36 cases of resected NSCLC. The mean age of the patients was 59.9+/-8.4 years. There were 19 cases of squamous cell carcinoma, 15 of adenocarcinoma and two of large cell carcinoma. We observed microsatellite instability at one or more loci in 13 (36%) of 36 tumors analyzed, and this instability ranged from six tumors showing instability in only a single microsatellite to three tumors that had alterations in three of four tested microsatellites. The microsatellite that showed instability most frequently in these tumors was D3S1340 (31%). Microsatellite instability was found in five (26%) of 19 squamous cell carcinomas, six (40%) of 15 adenocarcinomas, and in both large cell carcinomas tested. We found microsatellite instability in four (24%) of 17 cancers at stage I, in one (17%) of six at stage II, in eight (73%) of eleven at stage IIIa, and in neither at stage IIIb. In conclusion, microsatellite instability was noted in at least one third of non-small cell lung cancers, suggesting its possible role in cancer development.
Kim
C H
CH
Department of Internal Medicine and Lung Institute, College of Medicine, Seoul National University, South Korea.
Yoo
C G
CG
Han
S K
SK
Shim
Y S
YS
Kim
Y W
YW
D000230:Adenocarcinoma / Q000235:genetics; D000368:Aged; D018287:Carcinoma, Large Cell / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA