title: Viability of Chlamydia trachomatis in fallopian tubes of patients with ectopic pregnancy.
abstract: OBJECTIVE
  To use standard molecular methods to define the prevalence and metabolic characteristics of Chlamydia trachomatis during infection of fallopian tubes in women with ectopic pregnancies.
  
  
  METHODS
  Polymerase chain reaction (PCR)- and reverse transcription-PCR (RT-PCR)-based assessment of presence of chlamydial DNA and various RNA species in fallopian tube biopsy samples.
  
  
  METHODS
  Hospital and molecular genetics laboratory.
  
  
  METHODS
  Ten women of varying ages, each presenting with ectopic pregnancy.
  
  
  METHODS
  Positive signal in specific chlamydia-directed PCR and RT-PCR assays.
  
  
  RESULTS
  Nucleic acid preparations from 7 of the 10 fallopian tube patient samples were PCR-positive for C. trachomatis DNA. Each of the 7 PCR-positive samples also showed the presence of several transcripts from the bacterium, including primary transcripts from the ribosomal RNA operons.
  
  
  CONCLUSIONS
  A higher proportion of ectopic pregnancies than was believed previously may be attributable to infection of the fallopian tubes by C. trachomatis. The presence of various chlamydial RNA molecules suggests that viable, metabolically active bacteria were present in fallopian tubes of the patients studied.
authors.affiliation: Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
authors.forename: H C
authors.initials: HC
authors.lastname: Gérard
authors.forename: P J
authors.initials: PJ
authors.lastname: Branigan
authors.forename: G R
authors.initials: GR
authors.lastname: Balsara
authors.forename: C
authors.initials: C
authors.lastname: Heath
authors.forename: S S
authors.initials: SS
authors.lastname: Minassian
authors.forename: A P
authors.initials: AP
authors.lastname: Hudson
mesh_terms: D000328:Adult; D002692:Chlamydia trachomatis / Q000254:growth & development*; D005187:Fallopian Tubes / Q000382:microbiology*; D005260:Female; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction