title: Nicotinic acetylcholine subunit mRNA expression in dopaminergic neurons of the rat substantia nigra and ventral tegmental area.
abstract: The molecular composition of the nicotinic acetylcholine receptors (nAChRs) located on dopaminergic neurons and modulating their activity is unclear. Using the reverse transcriptase-polymerase chain reaction we have analyzed the mRNA for nAChR subunits expressed in the substantia nigra (SN) and ventral tegmental area (VTA) following unilateral 6-hydroxydopamine lesion of the dopaminergic system. In contrast to the unlesioned hemisphere, no signal was found in the lesioned hemisphere for alpha3, alpha5, alpha6 and beta4 subunits in the SN nor for alpha2, alpha3, alpha5, alpha6, alpha7 and beta4 subunits in the VTA, indicating the expression of these subunits in dopaminergic neurons. mRNA for alpha4, beta2 and beta3 subunits (and alpha7 in the SN) were still detected after lesion, suggesting that they are expressed in GABAergic neurons and interneurons of these brain areas. These results demonstrate the selective localisation of a number of nAChR subunit mRNA within dopaminergic neurons, strongly suggesting that a heterogenous population of nAChRs play a role in modulating dopaminergic neuronal activity.
authors.affiliation: Department of Genomic Biology, Synthélabo Recherche, Rueil Malmaison, France.
authors.forename: E
authors.initials: E
authors.lastname: Charpantier
authors.forename: P
authors.initials: P
authors.lastname: Barnéoud
authors.forename: P
authors.initials: P
authors.lastname: Moser
authors.forename: F
authors.initials: F
authors.lastname: Besnard
authors.forename: F
authors.initials: F
authors.lastname: Sgard
mesh_terms: D000818:Animals; D004298:Dopamine / Q000378:metabolism; D004333:Drug Administration Routes; D005260:Female; D007267:Injections; D008474:Medial Forebrain Bundle / Q000187:drug effects; D009474:Neurons / Q000737:chemistry* / Q000166:cytology; D016627:Oxidopamine / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011978:Receptors, Nicotinic / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013378:Substantia Nigra / Q000737:chemistry*; D017557:Ventral Tegmental Area / Q000737:chemistry*
chemical_list: D012333:RNA, Messenger; D011978:Receptors, Nicotinic; D016627:Oxidopamine; D004298:Dopamine