Production and characterization of a rabbit monoclonal antibody against human CDC25C phosphatase.
We produced a rabbit monoclonal antibody (MAb) against human CDC25C phosphatase. The antibody reacts with a minimal epitope between amino acids 291-295 in the highly conserved C-terminal region of CDC25C. The antibody recognizes denatured CDC25C of recombinant and mammalian origin in Western blot analysis. The corresponding rabbit polyclonal serum is able to immunoprecipitate the native protein, but this ability has been lost during the selection procedure. Although the production of the rabbit MAb requires more effort and patience than the mouse MAb technology, it offers a true alternative in case of antigens that are not immunogenic in mice.
Rief
N
N
Department of Medical Biochemistry and Molecular Biology, University of the Saarland, Homburg, Germany.
Waschow
C
C
Nastainczyk
W
W
Montenarh
M
M
Götz
C
C
D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins / Q000737:chemistry / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007527:Isoenzymes / Q000276:immunology; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000737:chemistry / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D020687:cdc25 Phosphatases
D000911:Antibodies, Monoclonal; D018797:Cell Cycle Proteins; D000939:Epitopes; D007527:Isoenzymes; D010749:Phosphoprotein Phosphatases; C517009:CDC25C protein, human; C517010:Cdc25c protein, mouse; D020687:cdc25 Phosphatases