title: Surface-enhanced Raman scattering and fluorescence spectroscopy reveal molecular interactions of all-trans retinoic acid and RAR gamma ligand-binding domain.
abstract: Surface-enhanced Raman scattering and fluorescence were used to investigate the interactions of all-trans retinoic acid with the gamma-type retinoic acid receptor. Raman data revealed a significant attenuation in intensity of the bands originating from the retinoic acid polyenic chain upon receptor binding, with the spectrum being dominantly that of the beta-ionone ring. Fluorescence measurements supported the hydrophobic character of the ligand binding. These novel spectroscopic results are fully consistent with the published X-ray crystallographic data and suggest that these techniques may be valuable additional tools to characterize the interactions of agonists and antagonists with residues in the ligand-binding pockets of retinoid receptor homo- and heterodimers.
authors.affiliation: Laboratoire de Spectroscopie Biomoléculaire, IFR53, UFR de Pharmacie, Université de Reims Champagne-Ardenne, France.
authors.forename: H
authors.initials: H
authors.lastname: Morjani
authors.forename: A
authors.initials: A
authors.lastname: Beljebbar
authors.forename: G D
authors.initials: GD
authors.lastname: Sockalingum
authors.forename: T A
authors.initials: TA
authors.lastname: Mattioli
authors.forename: D
authors.initials: D
authors.lastname: Bonnier
authors.forename: H
authors.initials: H
authors.lastname: Gronemeyer
authors.forename: M
authors.initials: M
authors.lastname: Manfait
mesh_terms: D001665:Binding Sites; D018360:Crystallography, X-Ray; D018168:Receptors, Retinoic Acid / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014212:Tretinoin / Q000737:chemistry* / Q000378:metabolism; D000097768:Retinoic Acid Receptor gamma
chemical_list: D018168:Receptors, Retinoic Acid; D014212:Tretinoin; D000097768:Retinoic Acid Receptor gamma