The three isoforms of transforming growth factor-beta co-stimulate rat T cells and inhibit lymphocyte apoptosis.
In this study the three different mammalian isoforms of transforming growth factor-beta (TGF-beta) were compared with regard to their effect on the response of rat T lymphocytes to the superantigen, staphylococcal enterotoxin A (SEA). All different isozymes were found to increase the proliferative response of rat T lymphocytes, which was accompanied by a significantly lower percentage of apoptotic cells than proliferation in the absence of TGF-beta. The same effect of TGF-beta was observed on the generation of apoptotic cells in an allo response (mixed lymphocyte reaction). TGF-beta2 and TGF-beta3 were three to 10-fold more potent than TGF-beta1 as co-stimulators of T lymphocytes, and equal in decreasing the percentage of apoptotic T cells. TGF-beta1 reduced the frequency and the number of cells undergoing apoptosis in T cells and, to an even higher degree, among B lymphocytes. TGF-beta did not seem to affect the production of the apoptosis inducer, tumour necrosis factor-alpha (TNF-alpha), neither at the mRNA level nor at the protein level. Neutralizing antibodies against the cytokine, TNF-alpha, decreased the percentage of apoptotic cells among T cells responding to SEA, both in the absence and in the presence of added TGF-beta1. Thus, when TGF-beta acts as a co-stimulator for T-cell activation it inhibits the induction of apoptosis and sustains the number of viable cells.
Schiött
A
A
Department of Tumor Immunology, University of Lund, Sweden.
Sjögren
H O
HO
Lindvall
M
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000276:immunology; D005434:Flow Cytometry; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D020033:Protein Isoforms / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000737:chemistry* / Q000494:pharmacology*
D020033:Protein Isoforms; D016212:Transforming Growth Factor beta