Both inhibin A and B respond to exogenous follicle-stimulating hormone in the follicular phase of the human menstrual cycle.
To ascertain whether changes in the concentrations of the dimeric inhibins A and/or B (INH-A and INH-B) contributed to the previously described dose-dependent increase in immunoreactive inhibin (INH) in response to FSH during the follicular phase of the human menstrual cycle, both dimers were measured by specific two-site assays in stored serum samples from regularly cycling normal volunteers who had received saline as a control (n = 5) or FSH [100 IU (n = 6) or 200 IU (n = 5)] between days 3-5 of the menstrual cycle. Both INH-A and INH-B showed a dose-dependent increase in response to administered FSH; INH-A rose from 13.5 to 35.9 ng/L (P < 0.01), and INH-B rose from 77.8 to 205 ng/L (P < 0.05) at 36 h after 200 IU FSH. Highly significant correlations were observed between INH and each of the specific inhibin dimers (A: r = 0.79, P < 0.001; B: r = 0.76, P < 0.001), and the responses of the two dimers were also highly correlated (r = 0.59, P < 0.001). The response of each inhibin was also highly correlated with the response of serum estradiol (A: r = 0.45, P < 0.001; B: r = 0.40, P < 0.001). When analyzed by ANOVA, the INH response of INH-B was significantly above the control value at 36 h after treatment with both 100 and 200 IU FSH, whereas the response of INH-A was significant only at 200 IU. It is concluded that the concentrations of both dimeric INH-A and INH-B are stimulated by increases in FSH within the physiological range in the follicular phase of the human menstrual cycle and that both contribute to the previously observed rise in INH.
Burger
H G
HG
Prince Henry's Institute of Medical Research, Monash Medical Center, Clayton, Victoria, Australia. henry.burger@med.monash.edu.au
Groome
N P
NP
Robertson
D M
DM
D000328:Adult; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D005498:Follicular Phase / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007265:Inhibins / Q000378:metabolism*; D008597:Menstrual Cycle / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism*; D012016:Reference Values; D012044:Regression Analysis
D020033:Protein Isoforms; D007265:Inhibins; D005640:Follicle Stimulating Hormone