title: Replacement of dietary saturated by unsaturated fatty acids: effects of platelet protein kinase C activity, urinary content of 2,3-dinor-TXB2 and in vitro platelet aggregation in healthy man.
abstract: The present study was conducted to compare the effects of oleic (OA) and linoleic acids (LA) on platelet function in healthy subjects. After a 4-week period on a diet rich in saturated fatty acids (SAFA), 38 volunteers (20 female, 18 male) had a high OA (18.0 en%) diet or a high LA diet (11.5 en%) for four weeks in a controlled manner. A control group of 13 subjects consumed their habitual diet throughout the study. Replacing the SAFA diet by the OA or LA diet did not affect the membrane-associated activity of platelet protein kinase C (PKC). However, both diets tended to increase the cytosolic activity to a comparable extent. Both the OA and the LA diets increased urinary excretion of 2.3-dinor-TXB2, as compared to the SAFA diet, whereas the urinary excretion of beta-thromboglobulin remained unchanged. As compared to the initial SAFA diet, platelet aggregation to collagen increased after both diets, while ADP induced aggregation showed no diet-induced changes. The results indicate increased platelet activity after both oil diets with no differences between the OA and LA diet and confirm in vitro findings that cis-unsaturated fatty acids have a distinct effect on cytosolic PKC, in particular.
authors.affiliation: Department of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, Finland. anu.turpeinen@helsinki.fi
authors.forename: A M
authors.initials: AM
authors.lastname: Turpeinen
authors.forename: A M
authors.initials: AM
authors.lastname: Pajari
authors.forename: R
authors.initials: R
authors.lastname: Freese
authors.forename: R
authors.initials: R
authors.lastname: Sauer
authors.forename: M
authors.initials: M
authors.lastname: Mutanen
mesh_terms: D000328:Adult; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D005227:Fatty Acids / Q000494:pharmacology*; D005260:Female; D006801:Humans; D019787:Linoleic Acid / Q000494:pharmacology*; D008297:Male; D019301:Oleic Acid / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects*; D011493:Protein Kinase C / Q000378:metabolism*; D013929:Thromboxane B2 / Q000031:analogs & derivatives* / Q000652:urine
chemical_list: D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D019301:Oleic Acid; D013929:Thromboxane B2; C028965:2,3-dinor-thromboxane B2; D019787:Linoleic Acid; D011493:Protein Kinase C