Identification and chromosomal localization of CTNNAL1, a novel protein homologous to alpha-catenin.
Sodium butyrate (NaB) was shown to induce growth inhibition and apoptosis in a human pancreatic cancer cell line (AsPC-1). To identify the genes that are differentially regulated by NaB, we generated subtracted EST libraries highly enriched for up- or down-regulated transcripts using the suppression subtraction hybridization technique. One of the ESTs identified in the down-regulated library showed significant homology to human alpha-catenin. A cDNA of 2.45 kb that codes for a putative protein of 734 aa was cloned. The cloned cDNA was used as a template in an in vitro transcription-translation reaction. This produced a protein product of about 82 kDa in size. The gene designated as CTNNAL1 (catenin (cadherin-associated protein), alpha-like 1) was found to be ubiquitously expressed in many tissues including pancreas, heart, and skeletal muscle. A human BAC clone containing the gene was isolated and used as a probe for fluorescence in situ hybridization (FISH). Both radiation hybrid and FISH analysis mapped the gene to chromosome band 9q31.2, a region where frequent abnormalities have been observed in bladder carcinoma, esophageal cancers, and several other tumors.
Zhang
J S
JS
Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester, Minnesota, 55905, USA.
Nelson
M
M
Wang
L
L
Liu
W
W
Qian
C P
CP
Shridhar
V
V
Urrutia
R
R
Smith
D I
DI
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002087:Butyrates / Q000494:pharmacology; D002874:Chromosome Mapping; D002899:Chromosomes, Human, Pair 9 / Q000235:genetics*; D003001:Cloning, Molecular; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018076:DNA, Complementary; D020224:Expressed Sequence Tags; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D010190:Pancreatic Neoplasms; D017386:Sequence Homology, Amino Acid; D014407:Tumor Cells, Cultured; D051178:alpha Catenin
D002087:Butyrates; C495273:CTNNA1 protein, human; C495274:CTNNAL1 protein, human; D003598:Cytoskeletal Proteins; D018076:DNA, Complementary; D051178:alpha Catenin