[Toxoplasma gondii: the characterization of an anti-P30 monoclonal antibody].
A specific monoclonal antibody was characterized to Toxoplasma gondii. The hybridoma produced IgG immunoglobulins. The western blot analysis showed that the monoclonal antibody was specific for the antigen of an apparent molecular mass of 30 kd, which was present on the antigen surface. The monoclonal antibody was purified starting from mouse's ascitic fluid and it was matched with sepharose 4B. This immunoabsorbent was used to purify the specific parasitic antigen. The monoclonal antibody studied may be useful for those techniques contributing to the toxoplasmosis diagnosis.
Fachado
A
A
Instituto de Medicina Tropical Pedro Kourí.
Fernández
N
N
Hernández
E
E
Fonseca
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000302:isolation & purification*; D000913:Antibodies, Protozoan / Q000032:analysis / Q000302:isolation & purification*; D000918:Antibody Specificity; D000953:Antigens, Protozoan / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D000939:Epitopes; D006825:Hybridomas / Q000276:immunology; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000276:immunology*; D011817:Rabbits; D014122:Toxoplasma / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D000954:Antigens, Surface; D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma