title: Structure and organization of the human reduced folate carrier gene.
abstract: The human reduced folate carrier gene was found to contain 7 exons, including two alternative non-coding exons (exons 1 and 2), spanning approximately 29 kb. Two transcript variants involving exon 7 were detected in K562 cells by RT-PCR, distinguishable from the wild-type transcript by deletions of 625 bp (KS32) and 988 bp (KS1). The presence of consensus splice donor and acceptor elements in the deleted KS1 isoform suggested that this form was likely a splice variant; however, KS32 likely arose during reverse transcription rather than by alternative splicing.
authors.affiliation: Experimental and Clinical Therapeutics Program, Karmanos Cancer Institute, 110 E. Warren Ave., Detroit, MI 48201, USA.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: S C
authors.initials: SC
authors.lastname: Wong
authors.forename: L H
authors.initials: LH
authors.lastname: Matherly
mesh_terms: D017398:Alternative Splicing; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D005091:Exons; D005492:Folic Acid / Q000378:metabolism; D006801:Humans; D007438:Introns; D020014:K562 Cells; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D058978:Reduced Folate Carrier Protein; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D014158:Transcription, Genetic
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D020033:Protein Isoforms; D058978:Reduced Folate Carrier Protein; C548699:SLC19A1 protein, human; C120043:SLC19A2 protein, human; D005492:Folic Acid