title: Antibodies to the chlamydial 60 kilodalton heat shock protein in women with tubal factor infertility.
abstract: BACKGROUND
  Fallopian tube damage and subsequent infertility are common sequelae of upper genital tract infection with Chlamydia trachomatis. This fallopian tube damage is thought to be immune mediated. The 60 kilodalton chlamydial heat shock protein (hsp) may be the key antigen associated with this pathogenic response. Our objective was to study the relationship between antibody response to 60 kilodalton chlamydial hsp and tubal factor infertility (TFI).
  
  
  METHODS
  Twenty-three women with TFI and 33 women with male factor infertility (controls) were studied. Tubal factor infertility was defined as infertility for one year with hydrosalpinx or distal tubal occlusion. Patients' sera were tested for antibodies to the chlamydial hsp using an enzyme-linked immunosorbent assay (ELISA). A stepwise logistic regression was performed by each patient's age, race/ethnicity, self-reported history of chlamydia infection, gonorrhea, or pelvic inflammatory disease (PID), history of ectopic pregnancy, and antibodies to the chlamydial hsp.
  
  
  RESULTS
  Eighteen of the 23 women with TFI had a positive result on the hsp ELISA (78.6%) versus 23.4% of controls. Risk factors for TFI were a history of PID (P = 0.022), "nonwhite" race (P = 0.004), history of ectopic pregnancy (P = 0.027), and antibodies to the 60 kilodalton chlamydial hsp (P < 0.001).
  
  
  CONCLUSIONS
  Antibodies to 60 kilodalton chlamydial hsp are strongly associated with TFI.
authors.affiliation: Department of Obstetrics and Gynecology, University of Kansas Medical Center, Kansas City, USA. kevin-ault@uiowa.edu
authors.forename: K A
authors.initials: KA
authors.lastname: Ault
authors.forename: B D
authors.initials: BD
authors.lastname: Statland
authors.forename: M M
authors.initials: MM
authors.lastname: King
authors.forename: D I
authors.initials: DI
authors.lastname: Dozier
authors.forename: M L
authors.initials: ML
authors.lastname: Joachims
authors.forename: J
authors.initials: J
authors.lastname: Gunter
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002692:Chlamydia trachomatis / Q000737:chemistry* / Q000276:immunology; D005184:Fallopian Tube Diseases / Q000276:immunology / Q000382:microbiology*; D005260:Female; D006360:Heat-Shock Proteins / Q000276:immunology*; D006801:Humans; D007247:Infertility, Female / Q000276:immunology / Q000382:microbiology*; D016015:Logistic Models; D008297:Male; D008970:Molecular Weight; D000292:Pelvic Inflammatory Disease / Q000382:microbiology; D011247:Pregnancy; D011271:Pregnancy, Ectopic / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D006360:Heat-Shock Proteins