Production of superoxide and nitric oxide by alveolar macrophages in the bleomycin-induced interstitial pneumonia mice model.
To elucidate the potential role of superoxide (O2-) and nitric oxide (NO) in the pathogenesis of interstitial pneumonia, the quantity of O2- and NO produced by the alveolar macrophages (AM) were determined in the bleomycin (BLM)-induced interstitial pneumonia mouse model. The production of O2- and NO increased on days 7, 14 and 21 after BLM injection. Strong expression of peroxynitrite (ONOO-) was seen in AM by using immunostaining for nitrotyrosine. The hydroxyproline contents increased on day 21 after BLM injection. O2- and NO are thought to play an important role in the pathology of fibrosis.
Yamazaki
C
C
Tokyo Research Institute, Seikagaku Corporation, Higashiyamato, Japan.
Hoshino
J
J
Sekiguchi
T
T
Hori
Y
Y
Miyauchi
S
S
Mizuno
S
S
Horie
K
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D001761:Bleomycin / Q000009:adverse effects*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D017563:Lung Diseases, Interstitial / Q000139:chemically induced / Q000378:metabolism*; D016676:Macrophages, Alveolar / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis*; D013481:Superoxides / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism
D000900:Anti-Bacterial Agents; D001761:Bleomycin; D013481:Superoxides; D009569:Nitric Oxide; C002744:3-nitrotyrosine; D014443:Tyrosine; D006909:Hydroxyproline