title: Clinical, virological and immune responses of normal and immunosuppressed donkeys (Equus asinus africanus) after inoculation with African horse sickness virus.
abstract: To elucidate the role that donkeys may play in African horse sickness virus (AHSV) persistence during inter-epizootic periods we looked for clinical signs of infection and studied the viraemia and neutralising antibody kinetics in 3 immunocompetent and 3 immunosuppressed donkeys inoculated with AHSV-4. None of the donkeys developed signs of AHS. However infectious AHSV was isolated from the blood of the immunocompetent donkeys for up to 17 days post infection (dpi) and viral antigens were detected for up to 28 dpi. Immune cells also increased significantly from 35 to 60 dpi. There was no evidence of a recrudescence of viraemia following immunosuppression of these donkeys at 90 dpi despite a decrease in the numbers of immune cells. Infectious virus was not isolated from the blood of donkeys that had been immunosuppressed, prior to AHSV inoculation. However viral antigens were detected for up to 35 dpi. The titres of AHSV-specific neutralising antibodies and the number of immune cells were also significantly lower than in immunocompetent animals. Our findings suggest that donkeys may be able to play a role in the epidemiology of AHS but the ability of vectors to become infected by feeding upon viraemic donkeys needs to be assessed before the significance of that role can be fully understood.
authors.affiliation: Département de Microbiologie, Immunologie et Maladies Contagieuses, Institut Agronomique et Vétérinaire Hassañ, Rabat-Instituts, Morocco.
authors.forename: O
authors.initials: O
authors.lastname: Fassi-Fihri
authors.forename: M
authors.initials: M
authors.lastname: el Harrak
authors.forename: M M
authors.initials: MM
authors.lastname: Fassi-Fehri
mesh_terms: D000355:African Horse Sickness / Q000453:epidemiology / Q000276:immunology* / Q000821:virology; D000356:African Horse Sickness Virus / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002460:Cell Line; D002522:Chlorocebus aethiops; D018397:Equidae; D005260:Female; D019085:Fluorescent Antibody Technique, Direct / Q000662:veterinary; D019084:Fluorescent Antibody Technique, Indirect / Q000662:veterinary; D007165:Immunosuppression Therapy / Q000662:veterinary; D007958:Leukocyte Count / Q000662:veterinary; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D009018:Morocco / Q000453:epidemiology; D009500:Neutralization Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012367:RNA, Viral / Q000097:blood; D014709:Vero Cells; D014766:Viremia / Q000276:immunology / Q000662:veterinary / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral