Increase in histamine production by inflammatory exudate in the chronic phase of allergic inflammation in rats.
In the air pouch-type allergic inflammation in rats, we reported that a sustained histamine production in the late phase is induced by a cytokine-like factor, named histamine-production-increasing factor (HPIF) (1). Recently, we found another type of histamine-production-increasing factor in the pouch fluid at the chronic phase of air pouch-type allergic inflammation. Although it did not increase histamine production by itself, it enhanced the HPIF-induced histamine production by rat bone marrow cells. It also increased GM-CSF-induced histamine production. The activity of this factor increased time-dependently from 3 to 7 days after the antigen challenge. Injection of the 5 day pouch fluid sample containing this factor into the pouch 4 h after the antigen challenge increased histamine contents in the pouch fluid at 24 h, indicating that this factor enhances HPIF-induced histamine production in vivo. Biochemical analysis of the 5 day pouch fluid sample indicated that this factor is a heat-labile and trypsin-sensitive protein of which pI value and molecular weight are 7-8 and about 100 kDa, respectively.
Hirasawa
N
N
Department of Pathophysiological Biochemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.
Shiraishi
M
M
Oikawa
S
S
Mue
S
S
Ohuchi
K
K
D000818:Animals; D002908:Chronic Disease; D004195:Disease Models, Animal; D005122:Exudates and Transudates / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006632:Histamine / Q000096:biosynthesis*; D006967:Hypersensitivity / Q000276:immunology*; D007249:Inflammation / Q000378:metabolism*; D007526:Isoelectric Point; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D011506:Proteins; D006632:Histamine; D016178:Granulocyte-Macrophage Colony-Stimulating Factor