Molecular analysis of phosphoglycerate kinase in Trypanoplasma borreli and the evolution of this enzyme in kinetoplastida.
In the protozoan kinetoplastid organism Trypanoplasma borreli, phosphoglycerate kinase (PGK) activity was found in two different cell compartments: 80% in the cytosol and 20% in peroxisome-like organelles called glycosomes. However, only one functional pgk gene could be detected, in addition to a pseudo-pgk gene. No short-range linkage could be established between these two genes, although they are presumably present on the same chromosome. The intact gene codes for a polypeptide of 411 amino acids, with a C-terminal extension of four residues, -VAKF, a sequence with probably a low targeting efficiency for glycosomes. The calculated net charge and molecular mass of the encoded polypeptide are +13 and 44230Da, respectively. In other Kinetoplastida, different tandemly arranged genes code for distinct PGK isoenzymes in glycosomes and cytosol. By comparison of the pgk gene organization, and a phylogenetic analysis, we have traced a plausible scenario of the evolution of the PGK isoenzymes in these organisms and of the enzymes' intracellular compartmentation.
Adjé
C A
CA
Research Unit for Tropical Diseases, Christian de Duve Institute of Cellular Pathology (ICP), Laboratory of Biochemistry, Catholic University of Louvain (UCL), Brussels, Belgium.
Opperdoes
F R
FR
Michels
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D019143:Evolution, Molecular; D005658:Fungi / Q000201:enzymology / Q000235:genetics; D016830:Kinetoplastida / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D010735:Phosphoglycerate Kinase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017384:Sequence Deletion; D017386:Sequence Homology, Amino Acid; D014351:Trypanosomatina / Q000201:enzymology / Q000235:genetics*
D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase