Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme-linked immunosorbent assay for the diagnosis of Brucella ovis in sheep.
The sensitivities of three commercial peroxidase conjugates (polyclonal anti-sheep IgG, recombinant protein G and a monoclonal anti-ruminant IgG1) in an enzyme-linked immunosorbent assay for Brucella ovis were evaluated. The monoclonal and protein G conjugates reduced, but did not totally remove, the characteristic background reactivity observed with the sera from brucella-free sheep. The protein G conjugate provided the best sensitivity, similar to that obtained with the classical gel diffusion test. Both tests were highly specific when testing sera from brucella-free animals but detected as positive a large proportion of sera from both Brucella melitensis-infected and B melitensis Rev 1-vaccinated sheep.
Marín
C M
CM
Unidad de Sanidad Animal, Diputación General de Aragón, Zaragoza, Spain.
Alonso-Urmeneta
B
B
Moriyón
I
I
Pérez-Gómez
S
S
Blasco
J M
JM
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D009419:Nerve Tissue Proteins / Q000276:immunology; D010544:Peroxidases; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology
D000907:Antibodies, Bacterial; C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D010544:Peroxidases