[Experimental studies and clinical significance of p53 gene mutation in carcinomas of the nasal cavity and paranasal sinuses].
By using PCR-SSCP method, 21 specimens of nasal cavity and paranasal sinuses carcinomas which were freshly frozen and paraffin embedded were detected for the mutation of p53 gene (exon 6). The overall mutation frequency of patients was 67% (14 out of 21), which was 33% (1 out of 3), 50% (3 out of 6), 67% (4 out of 6) and 100% (6 out of 6) respectively in clinical stage I to IV, 33% (2 out of 6), 67% (4 out of 6) and 89% (8 out of 9) respectively in well, moderately and poorly differentiated carcinomas, and 100% (4 out of 4) and 59% (10 out of 17) respectively in lymph-node metastasis and non-lymphnode metastasis. It was not statistically significant when the Chi-square test was applied (P > 0.05), but there was a tendency towards an increase in p53 mutation frequency with highly malignant, poorly differentiated and lymph-node metastasis carcinomas. The results indicate that the mutation of p53 gene plays an important role in the initial and progression of nasal cavity and praranasal sinuses carcinomas. Detection of p53 mutation using the above sensitive and rapid method reveals practical significance for judging malignant level and evaluating prognosis of nasal cavity and paranasal sinuses carcinomas.
Yang
W
W
Institute of Otolaryngology, General Hospital of PLA, Beijing.
Guo
W
W
Jiang
S
S
D000230:Adenocarcinoma / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D016158:Genes, p53; D006801:Humans; D008207:Lymphatic Metastasis; D009154:Mutation; D009296:Nasal Cavity; D009669:Nose Neoplasms / Q000235:genetics*; D010255:Paranasal Sinus Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational