Soluble Fc gamma receptors in periodontal lesions.
Soluble Fc gamma-binding components were detected in gingival fluid from periodontal lesions by incubation with biotinylated human Fc gamma fragments. Fc gamma III receptor was identified by incubation of gingival fluid with monoclonal antibody. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western transfer showed that most of the Fc gamma-binding components had minimal mobility in a 4-15% gradient gel under nonreducing conditions. Under reducing conditions, the main band of Fc gamma-binding components in gingival fluid migrated corresponding to protein A of 49 kDa. The pattern of Fc gamma-binding components was similar in serum and gingival fluid except for the observation in gingival fluid of Fc gamma-binding components migrating like standard proteins of 19 to 20 kDa, a size that corresponds to the polypeptide part of Fc gamma II receptor and Fc gamma III receptor.
Yuan
Z N
ZN
Department of Periodontology, University of Oslo, Norway.
Tolo
K
K
Helgeland
K
K
D015153:Blotting, Western; D002352:Carrier Proteins / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005883:Gingival Crevicular Fluid / Q000737:chemistry / Q000276:immunology; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D010518:Periodontitis / Q000097:blood / Q000276:immunology*; D011485:Protein Binding; D017452:Receptors, IgG / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D013205:Staphylococcal Protein A / Q000032:analysis
D002352:Carrier Proteins; D007141:Immunoglobulin Fc Fragments; D017452:Receptors, IgG; D013205:Staphylococcal Protein A