45Ca exchange in rat cortex slices in conditions of long-term potentiation.
Existing data on the role of Ca2+ ions in the development of long-term potentiation were used as a basis for studying changes in different Ca2+ compartments in cells in living rat olfactory cortex slices during potentiation. The kinetics of 45Ca2+ exchange were studied at 5, 15, and 30 min of potentiation. During the induction phase (1-5 min) of long-term potentiation, the fraction of tightly-bound intracellular Ca2+ decreased. There were no changes in the content of Ca2+ ions in other fractions at this stage. During maintenance of potentiation, which lasted 15-25 min, Ca2+ levels in the extracellular and intracellular compartments did not differ from controls. At 30 min, during extinction of long-term potentiation, there was a significant redistribution of Ca2+ in cells: the levels of free and loosely-bound Ca increased, as did extracellular Ca2+.
Sokolova
N E
NE
Laboratory of Functional Neurochemistry, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia.
Malikov
U M
UM
Kuznetsov
V L
VL
Emel'yanov
N A
NA
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002132:Calcium Radioisotopes; D002540:Cerebral Cortex / Q000378:metabolism* / Q000502:physiology*; D007700:Kinetics; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D009833:Olfactory Pathways / Q000378:metabolism / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D002132:Calcium Radioisotopes; D002118:Calcium