title: Subtraction radiography of dentinal caries-like lesions induced in vitro by cariogenic bacteria.
abstract: OBJECTIVE
  The objective of this project was to develop an in vitro model system for investigations of dentinal caries.
  
  
  METHODS
  Five extracted primary molar teeth with circular cavity preparations to the depth of the dentinoenamel junction were mounted individually in cold-cure acrylic bases constructed to fit a positioning jig in a Schick-Technologies digital radiographic imaging apparatus. The mounted teeth (MT) were incubated with pure cultures of Streptococcus mutans for 7 days and Lactobacillus casei for 38 days. At baseline and on day 23 and 45, four digital radiographs of each MT were made. Subtraction radiography was performed and analyzed using custom interactive software.
  
  
  RESULTS
  Lesions progressed roughly halfway through the dentin in all teeth at 23 days and approximated the pulp chambers at 45 days of incubation. Images resulting from subtraction procedures clearly revealed incremental caries progression which could be quantitated.
  
  
  CONCLUSIONS
  The model may be useful for screening cariostatic dental materials or treatments and investigating microbial mechanisms in dentinal caries.
authors.affiliation: Department of Oral and Craniofacial Biological Sciences (OCBS), University of Maryland Dental School, USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Minah
authors.forename: R H
authors.initials: RH
authors.lastname: Vandre
authors.forename: R
authors.initials: R
authors.lastname: Talaksi
mesh_terms: D002327:Cariostatic Agents / Q000627:therapeutic use; D002648:Child; D003731:Dental Caries / Q000000981:diagnostic imaging* / Q000382:microbiology; D003737:Dental Cavity Preparation; D003743:Dental Enamel; D003782:Dental Pulp / Q000000981:diagnostic imaging / Q000382:microbiology; D003804:Dentin / Q000000981:diagnostic imaging* / Q000382:microbiology; D018450:Disease Progression; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007780:Lacticaseibacillus casei / Q000502:physiology*; D008963:Molar / Q000000981:diagnostic imaging / Q000382:microbiology; D019252:Radiography, Dental, Digital; D012984:Software; D013295:Streptococcus mutans / Q000502:physiology*; D013382:Subtraction Technique; D014094:Tooth, Deciduous / Q000000981:diagnostic imaging / Q000382:microbiology
chemical_list: D002327:Cariostatic Agents