Starvation depresses acylglycerol biosynthesis in bovine subcutaneous but not intramuscular adipose tissue homogenates.
The primary objective of this study was to document the effects of starvation on acylglycerol biosynthesis in homogenates of intramuscular and subcutaneous adipose tissues. Adipose tissue samples were obtained from 8th-13th thoracic rib sections from 12 Angus cattle (six steers plus six heifers). Three steers and three heifers were starved for 72 h prior to slaughter while the remainder were slaughtered 4 h after food was withheld. Fat-free 700 x g centrifugal fractions were used to measure the esterification of radiolabeled sn-glycerol 3-phosphate (G-3-P) into acylglycerols at 1.0 mM palmitic or stearic acid, or 0.2 mM oleic, linoleic, or alpha-linolenic acid. There were significant tissue x fatty acid interactions for rates of incorporation into diacylglycerols and triacylglycerols; in subcutaneous, but not intramuscular homogenates, palmitic > stearic = oleic = linoleic = alpha-linolenic acid. Subcutaneous homogenates incorporated a greater percentage of G-3-P into triacylglycerols, and a lesser percentage into phospholipid, than intramuscular homogenates (P < 0.05). In intramuscular homogenates, the primary product of G-3-P esterification to saturated fatty acids was phospholipids. When unsaturated fatty acid served as substrates, triacylglycerols and phospholipids were produced in equal proportions in intramuscular homogenates, and triacylglycerols were the predominant product in subcutaneous homogenates. Intramuscular adipose tissue homogenates exhibited no response to starvation, whereas triacylglycerol and diacylglycerol synthesis was depressed by approximately 50% in subcutaneous adipose tissue homogenates. Similarly, phosphatidic phosphohydrolase activity, initially greater in subcutaneous than in intramuscular adipose tissue, was decreased by approximately 50% by starvation in subcutaneous adipose tissue, but not in intramuscular adipose tissue. We conclude that differences in rates of diacylglycerol and triacylglycerol biosynthesis, and response to starvation, between intramuscular and subcutaneous adipose tissues were due to dissimilarities in the activity of phosphatidic phosphohydrolase.
Smith
S B
SB
Department of Animal Science, Texas Agricultural Experiment Station, Texas A&M University, College Station 77843-2471, USA. sbs2185@acs.tamu.edu
Lin
K C
KC
Wilson
J J
JJ
Lunt
D K
DK
Cross
H R
HR
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005989:Glycerides / Q000096:biosynthesis*; D005994:Glycerophosphates / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010711:Phosphatidate Phosphatase / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D012867:Skin / Q000378:metabolism*; D013217:Starvation / Q000503:physiopathology*; D014280:Triglycerides / Q000096:biosynthesis
D005227:Fatty Acids; D005989:Glycerides; D005994:Glycerophosphates; D010743:Phospholipids; D014280:Triglycerides; C029620:alpha-glycerophosphoric acid; D010711:Phosphatidate Phosphatase