title: Suppression of channel conductance by diacetyl monoxime in guinea pig and embryonic chick cardiomyocytes.
abstract: OBJECTIVE
  To examine the effects of diacetyl monoxime (DAM), and putative dephospohrylating agent, on conductance of the cardiac Ca, Na, and K channels.
  
  
  METHODS
  The Ca (ICa), Na (INa), and K (IK) currents were recorded in single ventricular myocytes from guinea pigs and chick embryos before and after addition of DAM using the whole-cell voltage-clamp technique.
  
  
  RESULTS
  DAM 10 mmol.L-1 reduced rapidly the amplitudes of ICa (by about 30%), INa (by about 25%), and IK (by 25%-50%) without alterations of the voltage-dependence.
  
  
  CONCLUSIONS
  DAM was a channel inhibitor of the unique type having nonselective phosphatase activities.
authors.affiliation: Department of Pharmacology, Yamaguchi University, College of Medicine, Ube, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sada
authors.forename: T
authors.initials: T
authors.lastname: Ban
authors.forename: N
authors.initials: N
authors.lastname: Sperelakis
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002801:Cholinesterase Reactivators / Q000494:pharmacology*; D003931:Diacetyl / Q000031:analogs & derivatives* / Q000494:pharmacology; D006168:Guinea Pigs; D007473:Ion Channels / Q000187:drug effects*; D008564:Membrane Potentials; D009206:Myocardium / Q000166:cytology*; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000187:drug effects; D015222:Sodium Channels / Q000187:drug effects
chemical_list: D015220:Calcium Channels; D002801:Cholinesterase Reactivators; D007473:Ion Channels; D015221:Potassium Channels; D015222:Sodium Channels; C004717:diacetylmonoxime; D003931:Diacetyl