Cloning of the novel gene intelectin, which is expressed in intestinal paneth cells in mice.
Using a large-scale in situ hybridization screening method, we isolated the cDNA gene intelectin, whose mRNA is expressed in small intestinal paneth cells in mice. Northern blot analysis revealed that the mRNA corresponding to the cDNA was 1.2 kp in length, and expression was specific to the tissue in the small intestine. We termed this gene intelectin because the deduced amino acid sequence is similar to the previously cloned oocyte lectin gene of Xenopus laevis. The function of intelectin may be involved in the defence of microorganisms.
Komiya
T
T
Hirohashi Cell Configuration Project, ERATO, Japan Science and Technology Corporation (JST), 5-9-4 Tokodai, Tsukuba, 300-26, Japan. tkom@hccp.jst.go.jp
Tanigawa
Y
Y
Hirohashi
S
S
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015723:Gene Library; D017403:In Situ Hybridization; D007422:Intestines / Q000737:chemistry* / Q000166:cytology; D037102:Lectins / Q000235:genetics / Q000302:isolation & purification*; D051379:Mice; D008969:Molecular Sequence Data; D019879:Paneth Cells / Q000737:chemistry* / Q000166:cytology; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
D018076:DNA, Complementary; C115657:Itlnb protein, mouse; D037102:Lectins