Peroxisomal and microsomal fatty acid oxidation in liver of rats after chronic ethanol consumption.
1. Microsomal P450 and peroxisomal fatty acid oxidation activities were studied in liver of rats after long-term ethanol consumption. 2. Ethanol increased the microsomal lauric acid omega-hydroxylation and the aminopyrine N-demethylation catalyzed by cytochrome P450. 3. Ethanol increased peroxisomal beta-oxidation of palmitoyl CoA and catalase activity in liver. 4. Both microsomal and peroxisomal activities behaved in a coordinate way in the liver of rats with long-term ethanol consumption. 5. These results would support a role of microsomal omega-hydroxylation and peroxisomal beta-oxidation of fatty acids in an extramitochondrial pathway of lipid oxidation in the liver.
Orellana
M
M
ICBM, Programa de Farmacología, Molecular Y Clínica, Facultad de Medicina, Universidad de Chile, Santiago. morellan@machi.med.uchile.cl
Rodrigo
R
R
Valdés
E
E
D000428:Alcohol Drinking / Q000378:metabolism* / Q000473:pathology; D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D006900:Hydroxylation; D007850:Lauric Acids / Q000378:metabolism; D015227:Lipid Peroxidation; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000201:enzymology / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010171:Palmitoyl Coenzyme A / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D005227:Fatty Acids; D007850:Lauric Acids; D000632:Aminopyrine; C030358:lauric acid; D010171:Palmitoyl Coenzyme A; D003577:Cytochrome P-450 Enzyme System; D002374:Catalase