title: Cloning, characterization and expression of beta-N-acetylglucosaminidase gene from Streptomyces thermoviolaceus OPC-520(1).
abstract: The nagB gene encoding beta-N-acetylglucosaminidase from S. thermoviolaceus OPC-520 was cloned and sequenced. The nagB gene could encode a protein of 541 amino acids with a calculated molecular mass of 58274. NagB revealed significant similarities to beta-N-acetylhexosaminidases and chitobiases from bacteria, which are classified into family 20 glycosyl hydrolases. NagB effectively hydrolyzed all of the chitin oligosaccharides from dimer to hexamer.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan. tsujibo@oysun01.oups.ac.jp
authors.forename: H
authors.initials: H
authors.lastname: Tsujibo
authors.forename: N
authors.initials: N
authors.lastname: Hatano
authors.forename: T
authors.initials: T
authors.lastname: Mikami
authors.forename: Y
authors.initials: Y
authors.lastname: Izumizawa
authors.forename: K
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Inamori
mesh_terms: D000118:Acetylglucosaminidase / Q000096:biosynthesis / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D002686:Chitin / Q000378:metabolism; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology / Q000235:genetics*
chemical_list: D002686:Chitin; D000118:Acetylglucosaminidase