[The epidemiologic value of polymerase chain reaction to the diagnosis of brucellosis patients].
PCR was used to test fifty-four brucellasis patients and thirty-six healthy people as controls, who were all from Man Tang Chuan and Yi He communities, Sui De county, Shan Xi Province, where brucellosis out-break was occurred in 1996. The diagnostic titres of SAT for brucellasis patients was set 1:100++. The positive rates of PCR in patients and controls were 75% and 65.7% respectively, comparing with the positive rates of RBPT 96.3% and 30.6% in the same two groups. At the same time, we also detected eight people who had received immunization one month to thirty-two years ago, with positive rates for PCR and RBPT 100% and 87.5% respectively. The results showed that there was no significant diversity of PCR results among acute, subacute and chronic brucellosis patients. It was revealed that the serological results were related to the course of disease, but the results came out of PCR method which was used to detect target DNA was not influenced by the course of disease.
Tang
L Y
LY
Institute of Epidemiology & Microbiology, Chinese Academy Preventive Medicine, Beijing.
Qiu
H Y
HY
Li
Y K
YK
D000818:Animals; D002006:Brucellosis / Q000175:diagnosis* / Q000453:epidemiology*; D002681:China / Q000453:epidemiology; D005069:Evaluation Studies as Topic; D006801:Humans; D016133:Polymerase Chain Reaction; D012698:Serologic Tests