[Genotoxic and clastogenic effects of doxorubicin].
Doxorubicine is an anthracyclin used widely in medical oncology. The purpose of this investigation was to evaluate the genotoxic and clastogenic effects of this substance through the cytokinesis-block micronucleus assay. This short-term mutagenicity assay is of easy realization and quick interpretation. It has enabled the demonstration of a significant decrease, after exposure of cells to doxorubicin, of the rate of micronucleated cells (average = 10.8 +/- 5.7 micronucleus versus 31.9 +/- 11.5 after exposure, p < 0.0001) and chromosomic aberrations (1 aberration for the control culture versus 17 since the first dose, p < 0.001). At the end of this study, the cytokinesis-block micronucleus assay represents a reliable test to study and evaluate the genotoxic power of some substances.
Villani
P
P
Laboratoire de Biogénotoxicologie (EA 1784), Faculté de Médecine, Marseille, France.
Orsière
T
T
Duffaud
F
F
Digue
L
L
Bouvenot
G
G
Botta
A
A
D000328:Adult; D015197:Carcinogenicity Tests; D002273:Carcinogens / Q000633:toxicity*; D004317:Doxorubicin / Q000633:toxicity*; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*
D002273:Carcinogens; D009153:Mutagens; D004317:Doxorubicin