Clustering of human chromosome fragments on the mouse genome by chromosome-mediated gene transfer.
Pieces of metaphase chromosomes prepared from mouse cells containing neo-tagged human chromosome 7 were transferred to mouse cells with calcium phosphate to isolate G418-resistant clones. FISH analysis revealed that the majority of them contained human DNA at a single site on their genome. These transformants contained STS markers mapped to various regions of chromosome 7. It is thus suggested that pieces of human chromosomes tend to assemble and integrate on the mouse genome.
Ogino
H
H
Division of Biochemistry, Kihara Institute for Biological Research, and Graduate School of Integrated Science, Yokohama City University, Yokohama, Japan.
Nakabayashi
K
K
Michishita
E
E
Scherer
S W
SW
Fujii
M
M
Suzuki
T
T
Ayusawa
D
D
D000818:Animals; D001483:Base Sequence; D002877:Chromosomes, Human; D017931:DNA Primers; D018014:Gene Transfer Techniques; D016678:Genome; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D051379:Mice
D017931:DNA Primers