A novel screening method for isolating exopolysaccharide-deficient mutants.
A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.
Liu
M
M
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
González
J E
JE
Willis
L B
LB
Walker
G C
GC
D001391:Azo Compounds; D001431:Bacteriological Techniques; D004396:Coloring Agents; D005821:Genetic Techniques; D009281:Naphthalenes; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D016962:Sinorhizobium meliloti / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification*
D001391:Azo Compounds; D004396:Coloring Agents; D009281:Naphthalenes; D011135:Polysaccharides, Bacterial; C056779:succinoglycan; C016118:Sudan Black B