Autoantibodies to CD45 in systemic lupus erythematosus.
Western blotting with U937 cell extracts as the substrate, and enzyme-linked immunosorbent assays (ELISA) with U937-, Jurkat- and Daudi cell-purified CD45 molecules were used to detect anti-CD45 reactivity in patients with systemic lupus erythematosus (SLE). By immunoblotting, 16 of 64 SLE sera were shown to be positive (25.0%). In the ELISAs, 13 out of 18 SLE sera reacted with the target CD45. Of these, three were not detectable on the blot. Importantly, 12 of these ELISA-positive sera contained IgM and IgG auto-antibodies. Neuraminidase-treatment of U937-precipitated CD45 molecules enhanced the reactivity to most of the isoforms, indicating that the antibodies may bind to asialylated polysaccharides.
Mamoune
A
A
Laboratory of Immunology, Unit of Rheumatology, Institut de Synergie des Sciences et de la Santé, Brest University Medical School, Brest, France.
Saraux
A
A
Delaunay
J L
JL
Le Goff
P
P
Youinou
P
P
Le Corre
R
R
D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D002460:Cell Line; D005260:Female; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000097:blood; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000276:immunology* / Q000302:isolation & purification; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D020298:U937 Cells
D001323:Autoantibodies; D007132:Immunoglobulin Isotypes; D017493:Leukocyte Common Antigens