Experimental protocol for clinical analysis of cerebrospinal fluid by high resolution proton magnetic resonance spectroscopy.
High resolution magnetic resonance spectroscopy (MRS) of cerebrospinal fluid (CSF) is a non-destructive analytical method which allows rapid and simultaneous detection of molecules involved in intermediary and oxidative metabolic pathways. We developed a protocol suitable for routine MRS analysis of lyophilized CSF samples. This procedure guarantees sample integrity, from CSF collection to spectrum acquisition. MRS analysis of blood serum was included in our protocol as a complementary method to CSF analysis. This protocol can contribute to establish MRS of CSF as a new analytical tool to better understand the metabolic processes involved in neurological diseases.
Maillet
S
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Centre de Résonance Magnétique Biologique et Médicale, UMR CNRS 6612, Faculté de Médecine, 27 Bd Jean Moulin, F-13005, Marseille, France.
Vion-Dury
J
J
Confort-Gouny
S
S
Nicoli
F
F
Lutz
N W
NW
Viout
P
P
Cozzone
P J
PJ
D000328:Adult; D000368:Aged; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry / Q000502:physiology; D002555:Cerebrospinal Fluid / Q000737:chemistry / Q000378:metabolism; D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction
D002556:Cerebrospinal Fluid Proteins