A rapid and sensitive method for detecting human cells in xenogeneic hosts.
Xenografts, specifically transplantation of human cells into other species, are a valuable tool in preclinical transplantation experiments. A central issue is accurate identification of the grafted cells, particularly in cases in which cellular migration has occurred. We report that detection of grafted human cells can be achieved by in situ hybridization techniques using human centromeric probes which result in unambiguous nuclear labeling. The resulting reaction can be combined with immunocytochemical or histochemical techniques for cell-type characterization.
Zompa
E A
EA
Marine Biomedical Institute and Department of Anatomy and Neurosciences, The University of Texas Medical Branch, Galveston 77555-1069, USA.
Moyer
M P
MP
Cain
L D
LD
Bottenstein
J E
JE
Hulsebosch
C E
CE
D000818:Animals; D017690:Cell Transplantation; D015342:DNA Probes; D005260:Female; D016332:Fetal Tissue Transplantation; D005333:Fetus / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D013116:Spinal Cord / Q000196:embryology / Q000473:pathology* / Q000601:surgery*; D013997:Time Factors; D014183:Transplantation, Heterologous
D015342:DNA Probes