title: Purification of carp (Cyprinus carpio) hepatopancreatic lactate dehydrogenase (EC 1.1.1.27).
abstract: Lactate dehydrogenase (LDH, EC 1.1.1.27) was isolated in 86% purity from carp (Cyprinus carpio) hepatopancreas using agarose T-gel (mercaptoethanol bound to agarose via divinylsulphone) affinity chromatography medium and Iontosorb phenyl hydrophobic chromatography medium. Gel filtration chromatography of the LDH obtained shows molecular weight to be 26,900 Da.
authors.affiliation: Food Research Institute, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Kminková
authors.forename: J
authors.initials: J
authors.lastname: Kucera
mesh_terms: D000818:Animals; D002347:Carps; D002645:Chickens; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification*; D008099:Liver / Q000201:enzymology*; D018482:Muscle, Skeletal / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010179:Pancreas / Q000201:enzymology*; D011817:Rabbits
chemical_list: D007770:L-Lactate Dehydrogenase