Fiber capillarization and ultrastructure of pigeon pectoralis muscle after cold acclimation.
We investigated the effect of 2 months of exposure to cold conditions (0-5 C) on capillarization and on fiber size, distribution and ultrastructure in the pectoralis muscle of nine pigeons (Columbia livia; mean body mass 700 31 g) and compared the results with measurements from four control birds (mean mass 715 42 g) kept at normal ambient temperature (22-23 C) for the same period. Superficial and deep portions of the muscles, taken from the central area of the right or left pectoralis major muscle, were perfusion-fixed in situ, processed for electron microscopy and analyzed by morphometry. Aerobic fibers represented the vast majority of fibers (93 1 %, mean s.e.m.) in all samples. After cold-acclimation, fiber sectional area was reduced and capillary density increased proportionally. There was no change in the degree of orientation (anisotropy) of capillaries, capillary-to-fiber ratio or fiber type distribution compared with controls. The volume density of mitochondria and lipid droplets in aerobic fibers and capillary diameter increased in response to cold, while the linear relationship between capillary length per fiber volume and fiber mitochondrial volume density remained unchanged. Capillary surface area, intrafiber lipid deposition and fiber mitochondrial volume density were all correlated in cold-acclimated pigeons. The results indicate a close match between the aerobic capacity of the highly aerobic fibers of the pectoralis muscle and their vascularization to meet the increased energetic demand of shivering.
Mathieu-Costello
O
O
Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0623, USA and Department of Biology, New Mexico State University, Las Cruces, NM 88003, USA. odile@ucsd.edu.
Agey
P J
PJ
Quintana
E S
ES
Rousey
K
K
Wu
L
L
Bernstein
M H
MH
D000064:Acclimatization / Q000502:physiology*; D000332:Aerobiosis; D000818:Animals; D002196:Capillaries / Q000648:ultrastructure; D003080:Cold Temperature; D010856:Columbidae / Q000502:physiology; D004734:Energy Metabolism; D050356:Lipid Metabolism; D008931:Mitochondria, Muscle / Q000502:physiology / Q000648:ultrastructure; D018485:Muscle Fibers, Skeletal / Q000648:ultrastructure; D010101:Oxygen Consumption; D010369:Pectoralis Muscles / Q000098:blood supply / Q000502:physiology* / Q000648:ultrastructure