Quantitative analysis of the immunopotency of genetically transfected dendritic cells.
Dendritic cells (DCs) instruct and activate a naive immune system to mount a response toward foreign proteins. Therefore, it has been hypothesized that an ideal vaccine strategy would be to directly introduce genes encoding antigens into DCs. To test this strategy quantitatively, we have compared the immune response elicited by a genetically transfected DC line to that induced by a fibroblast line, or standard genetic immunization. We observe that a single injection of 500-1,000 transfected DCs can produce a response comparable to that of standard genetic immunization, whereas fibroblasts, with up to 50-fold greater transfection efficiency, were less potent. We conclude that transfection of a small number of DCs is sufficient to initiate a wide variety of immune responses. These results indicate that targeting genes to DCs will be important for controlling and augmenting the immunological outcome in genetic immunization.
Timares
L
L
Department of Medicine, University of Texas, Southwestern Medical Center, Dallas, TX 75235-8573, USA.
Takashima
A
A
Johnston
S A
SA
D000818:Animals; D000831:Animals, Newborn; D000938:Antigen-Presenting Cells / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D049452:Green Fluorescent Proteins; D007114:Immunization / Q000379:methods; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008805:Mice, Inbred A; D011994:Recombinant Proteins / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000276:immunology*; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis / Q000235:genetics
D008164:Luminescent Proteins; D011994:Recombinant Proteins; D000515:alpha 1-Antitrypsin; D049452:Green Fluorescent Proteins; D008156:Luciferases