Primed in situ labeling (PRINS): a method for rapid identification and quantification of human chromosomes in both lymphocytes and sperm nuclei.
In this work, a specific primer for X alphoid satellite DNA was used to detect chromosome X through primed in situ labeling (PRINS). The method allows the rapid identification of chromosome X in metaphase and its quantification in interphase. PRINS is equally applicable to both lymphocytes and sperm nuclei.
Musio
A
A
Dipartimento di Scienze dell'Uomo e dell'Ambiente, Università degli studi di Pisa, Italy. musio@desiolab.unimi.it
Baroli
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P
Sbrana
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I
D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D005260:Female; D006801:Humans; D007399:Interphase / Q000235:genetics; D008214:Lymphocytes / Q000648:ultrastructure*; D008297:Male; D008677:Metaphase / Q000235:genetics; D020401:Primed In Situ Labeling / Q000379:methods*; D013077:Sperm Head / Q000648:ultrastructure*; D014960:X Chromosome / Q000235:genetics*
D017931:DNA Primers