title: DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).
abstract: The analysis of cattle MHC (BoLA) class I gene expression is an essential component of studies on immune responses and susceptibility to disease. International BoLA workshops have generated data and reagents that allow discrimination of class I molecules at the haplotype level, but progress has been limited by difficulties encountered in defining single alleles. Our aim in this study was to develop a DNA-based system for improved identification of expressed class I alleles, utilizing available cDNA sequences derived from cattle carrying a series of serologically defined class I specificities. This method has allowed more accurate typing of animals for expression of the class I genes present within a small number of haplotypes. The method has also reliably differentiated between allelic variants (identified by prior sequence analysis) and has split existing serological specificities. The data show that MHC class I genes in cattle are more polymorphic than demonstrated by serology and biochemical analysis.
authors.affiliation: Institute for Animal Health, Compton Laboratory, UK.
authors.forename: S A
authors.initials: SA
authors.lastname: Ellis
authors.forename: K A
authors.initials: KA
authors.lastname: Staines
authors.forename: M J
authors.initials: MJ
authors.lastname: Stear
authors.forename: E J
authors.initials: EJ
authors.lastname: Hensen
authors.forename: W I
authors.initials: WI
authors.lastname: Morrison
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D004247:DNA / Q000145:classification; D017931:DNA Primers; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D017931:DNA Primers; D015395:Histocompatibility Antigens Class I; D004247:DNA