title: Sequence of a second gene encoding bovine submaxillary mucin: implication for mucin heterogeneity and cloning.
abstract: Secreted epithelial mucins are extremely large and heterogeneous glycoproteins. We report the 5 kilobase DNA sequence of a second gene, BSM2, which encodes bovine submaxillary mucin. The determined nucleotide and deduced amino acid sequences of BSM2 are 95.2% and 92. 2% identical, respectively, to those of the previously described BSM1 gene isolated from the same cow. Further, the five predicted protein domains of the two genes are 100%, 94%, 93%, 77%, and 88% identical. Based on the above results, we propose that expression of multiple homologous core proteins from a single animal is a factor in generating diversity of saccharides in mucins and in providing resistance of the molecules to proteolysis. In addition, this work raises several important issues in mucin cloning such as assembling sequences from seemingly overlapping clones and deducing consensus sequences for nearly identical tandem repeats.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, 17033, USA. wjiang@psu.edu
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: J T
authors.initials: JT
authors.lastname: Woitach
authors.forename: D
authors.initials: D
authors.lastname: Gupta
authors.forename: V P
authors.initials: VP
authors.lastname: Bhavanandan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D015698:Genomic Library; D008969:Molecular Sequence Data; D009077:Mucins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D020449:Repetitive Sequences, Amino Acid; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: C115439:BSM1 protein, Bos taurus; C115440:BSM2 protein, Bos taurus; D009077:Mucins; D011994:Recombinant Proteins