No effects of low copper intake on rat mammary tissue superoxide dismutase 1 activity and mammary chemical carcinogenesis.
Variations in copper-containing superoxide dismutase (SOD) 1 are hypothesized to produce variations in resistance to carcinogenesis, particularly in mammary tissue. Therefore, it is reasonable to speculate that low copper intake, which causes low SOD 1 activities in various tissues, would cause poor resistance to mammary carcinogenesis. This idea was tested using female rats fed diets either low or adequate in copper (< 0.5 or 8 mg copper/kg diet) plus or minus oral gavage with the mammary carcinogen 7,12-dimethylbenz[a]anthracene (5 mg/kg, given 5 wk after dietary modification, 28 wk before sacrifice). Low copper intake produced low activities of two serum copper enzymes: ceruloplasmin and extracellular SOD. In contrast, low copper intake did not affect mammary tissue SOD 1 activities, nor did it statistically influence any of several parameters of 7,12-dimethylbenz[a]anthracene-induced mammary carcinogenesis.
DiSilvestro
R A
RA
Department of Human Nutrition and Food Management, Ohio State University, Columbus 43210-1295, USA. disilvestro.1@osu.edu
Sakamoto
K
K
Milner
J A
JA
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002273:Carcinogens; D003300:Copper / Q000172:deficiency*; D004032:Diet; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism*; D000072105:Superoxide Dismutase-1
D002273:Carcinogens; C000606290:SOD1 protein, human; D015127:9,10-Dimethyl-1,2-benzanthracene; D003300:Copper; C000606292:Sod1 protein, rat; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1