title: Dietary administration of an extract from rosemary leaves enhances the liver microsomal metabolism of endogenous estrogens and decreases their uterotropic action in CD-1 mice.
abstract: We evaluated the effects of a methanol extract from the leaves of the plant Rosmarinus officinalis L. (rosemary) on the metabolism and action of estradiol and estrone. Treatment of female CD-1 mice with 2% rosemary in AIN-76A diet for 3 weeks increased the liver microsomal 2-hydroxylation of estradiol and estrone by approximately 150%, increased their 6-hydroxylation by approximately 30% and inhibited the 16alpha-hydroxylation of estradiol by approximately 50%. Treatment of female CD-1 mice with 2% rosemary diet for 3 weeks also stimulated the liver microsomal glucuronidation of estradiol and estrone by 54-67% and 37-56%, respectively. In additional studies, feeding 2% rosemary diet to ovariectomized CD-1 mice for 3 weeks inhibited the uterotropic action of estradiol and estrone by 35-50% compared with animals fed a control diet. The results of this study showed that feeding female mice a 2% rosemary diet increased the liver microsomal oxidation and glucuronidation of estradiol and estrone and inhibited their uterotropic action.
authors.affiliation: Department of Chemical Biology, College of Pharmacy, Rutgers--The State University of New Jersey, Piscataway 08854-8020, USA.
authors.forename: B T
authors.initials: BT
authors.lastname: Zhu
authors.forename: D P
authors.initials: DP
authors.lastname: Loder
authors.forename: M X
authors.initials: MX
authors.lastname: Cai
authors.forename: C T
authors.initials: CT
authors.lastname: Ho
authors.forename: M T
authors.initials: MT
authors.lastname: Huang
authors.forename: A H
authors.initials: AH
authors.lastname: Conney
mesh_terms: D000818:Animals; D004032:Diet; D004967:Estrogens / Q000378:metabolism* / Q000502:physiology; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D006900:Hydroxylation; D019686:Lamiaceae / Q000737:chemistry*; D051379:Mice; D008862:Microsomes, Liver / Q000187:drug effects* / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D039181:Steroid 16-alpha-Hydroxylase; D014599:Uterus / Q000502:physiology*
chemical_list: D004967:Estrogens; D005965:Glucuronates; D010936:Plant Extracts; D009249:NADP; D039181:Steroid 16-alpha-Hydroxylase