title: Accessibility of SSA/Ro and SSB/La antigens to maternal autoantibodies in apoptotic human fetal cardiac myocytes.
abstract: Access of intracellular Ags SSA/Ro and SSB/La to cognate maternal autoantibodies is unexplained despite their strong association with congenital heart block. To investigate the hypothesis that apoptosis facilitates surface accessibility of these Ags, human fetal cardiac myocytes from 16- to 22-wk abortuses were established in culture using a novel technique in which cells were isolated after perfusing the aorta with collagenase. Confirmation of cardiac myocytes included positive staining with antisarcomeric alpha-actinin and contractility induced by 1.8 mM calcium. Incubation with 0.5 microM staurosporine or 0.3 mM 2,3-dimethoxy-1,4-naphthoquinone induced the characteristic morphologic and biochemical changes of apoptosis. The cellular topology of Ro and La was evaluated with confocal microscopy and determined in nonapoptotic and apoptotic cardiocytes by indirect immunofluorescence. In permeabilized nonapoptotic cardiocytes, Ro and La were predominantly nuclear, and propidium iodide (PI) stained the nucleus. In early apoptotic cardiocytes, condensation of the PI- and Ro- or La-stained nucleus was observed, accompanied by Ro/La fluorescence around the cell periphery. In later stages of apoptosis, nuclear Ro and La staining became weaker, and PI demonstrated nuclear fragmentation. Ro/La-stained blebs emerged from the cell membrane, a finding observed in nonpermeabilized cells, supporting an Ab-Ag interaction at the cell surface. In summary, induction of apoptosis in cultured cardiocytes results in surface translocation of Ro/La and recognition by Abs. Although apoptotic cells are programmed to die and do not characteristically evoke inflammation, binding of maternal Abs and subsequent influx of leukocytes could damage surrounding healthy fetal cardiocytes.
authors.affiliation: Hospital for Joint Diseases, New York University School of Medicine, NY 10003, USA.
authors.forename: M E
authors.initials: ME
authors.lastname: Miranda
authors.forename: C E
authors.initials: CE
authors.lastname: Tseng
authors.forename: W
authors.initials: W
authors.lastname: Rashbaum
authors.forename: R L
authors.initials: RL
authors.lastname: Ochs
authors.forename: C A
authors.initials: CA
authors.lastname: Casiano
authors.forename: F
authors.initials: F
authors.lastname: Di Donato
authors.forename: E K
authors.initials: EK
authors.lastname: Chan
authors.forename: J P
authors.initials: JP
authors.lastname: Buyon
mesh_terms: D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000276:immunology; D001011:Aorta / Q000196:embryology; D017209:Apoptosis; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology*; D001692:Biological Transport; D002467:Cell Nucleus / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D017364:Collagenases / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005318:Fetal Heart / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D005326:Fetal Proteins / Q000276:immunology*; D006327:Heart Block / Q000151:congenital* / Q000209:etiology; D006801:Humans; D007112:Immunity, Maternally-Acquired; D009124:Muscle Proteins / Q000276:immunology; D009206:Myocardium / Q000166:cytology / Q000276:immunology*; D009285:Naphthoquinones / Q000494:pharmacology; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology*; D020033:Protein Isoforms / Q000276:immunology; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins / Q000276:immunology*; D019311:Staurosporine / Q000494:pharmacology; D000097585:SS-B Antigen; D000099207:SS-A Antigen
chemical_list: D000954:Antigens, Surface; D001323:Autoantibodies; D001324:Autoantigens; D017364:Collagenases; D004791:Enzyme Inhibitors; D005326:Fetal Proteins; D009124:Muscle Proteins; D009285:Naphthoquinones; D020033:Protein Isoforms; D020733:RNA, Small Cytoplasmic; D012261:Ribonucleoproteins; D019311:Staurosporine; D000097585:SS-B Antigen; D000099207:SS-A Antigen; C116006:RO60 protein, human; C063002:2,3-dimethoxy-1,4-naphthoquinone