[Inhibition of sodium selenite on transformation of human B lymphocytes by Epstein-Barr virus].
We detected the effect of non-toxic doses of sodium selenite (Se) on the expressing effect of nuclear antigen (EBNA) of Epstein-Barr virus (EBV) in Raji cell line by microfluorescence spectrophoto-quantitation (MFS) and studied the inhibing transformation in B lymphocytes from human umbilical cord blood (BL) with EBV in vitro by Se. We found that Se (0.01-0.50 microgram/ml) can lower EBNA-MFS value 8.4%-21.8% in Raji cell line. The transformation effect of BL with EBV was markedly inhibited by Se (0.1-1.0 microgram/ml) in pretreatment and cotreatment. The inhibition rates of BL transformation were 73.4%-92.1% and 60.3%-77.2% respectively. EBV is associated with nasopharyngeal carcinoma (NPC). The Se levels of serum and hair in NPC patients were markedly lower than those in healthy persons. The results suggest that supplement Se to persons for active expression of EBV gene and NPC patients may help protect againt and treat NPC and diseases associated with EBV.
Luo
H
H
Cancer Institute, Sun Yat-Sen University of Medical Sciences, Guangzhou.
Wu
Y
Y
Li
M
M
D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000821:virology; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis*; D004854:Herpesvirus 4, Human; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D009303:Nasopharyngeal Neoplasms / Q000517:prevention & control; D018038:Sodium Selenite / Q000494:pharmacology*
D016588:Anticarcinogenic Agents; D019309:Epstein-Barr Virus Nuclear Antigens; D018038:Sodium Selenite