Diacylglycerol pyrophosphate: a novel metabolite in the Trypanosoma cruzi phosphatidic acid metabolism.
This work provides evidence that phosphatidic acid (PA) is metabolized to diacylglycerol pyrophosphate (DGPP) in Trypanosoma cruzi. Also the presence of the enzymatic activities involved in its regulation, phosphatidate kinase (PA-k) and phosphatidate phosphatase, is demonstrated. The increase of DGPP levels in T. cruzi epimastigotes or in its membrane fraction after exogenous PA addition or phospholipase (PLD) pre-treatment suggests that PA-k may be involved in the regulation of PA levels after its stimulation.
Marchesini
N
N
Química Biológica, Facultad de Ciencias Exactas, Fisico-Químicas y Naturales, Universidad Nacional de Rio Cuarto, Córdoba, Argentina.
Santander
V
V
Machado-Domenech
E
E
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D007700:Kinetics; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000378:metabolism*; D017856:Phosphotransferases (Phosphate Group Acceptor) / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000378:metabolism*
D010712:Phosphatidic Acids; D017856:Phosphotransferases (Phosphate Group Acceptor); C099834:phosphatidic acid kinase; D010711:Phosphatidate Phosphatase