title: An agent that increases tumor suppressor transgene product coupled with systemic transgene delivery inhibits growth of metastatic lung cancer in vivo.
abstract: Low levels of gene expression following systemic delivery have impaired the effectiveness of tumor suppressor gene replacement in treating metastases. We asked whether combined treatment with 2-methoxyestradiol (2-Me), which increases levels of wild-type p53 protein in cancer cells, and the systemic administration of an adenoviral vector expressing wild-type p53 (Ad-p53) would inhibit the growth of human metastatic lung cancer cells in vivo. The simultaneous administration of p53 and 2-Me resulted in a greater than additive reduction with the lung colony count reduced to 33% of its control value. These results suggest that the synergistic effect of 2-Me and Ad-p53 in combination treatment may have application in the systemic treatment of cancer.
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kataoka
authors.forename: G
authors.initials: G
authors.lastname: Schumacher
authors.forename: R J
authors.initials: RJ
authors.lastname: Cristiano
authors.forename: E N
authors.initials: EN
authors.lastname: Atkinson
authors.forename: J A
authors.initials: JA
authors.lastname: Roth
authors.forename: T
authors.initials: T
authors.lastname: Mukhopadhyay
mesh_terms: D000077584:2-Methoxyestradiol; D000256:Adenoviridae / Q000235:genetics; D004958:Estradiol / Q000031:analogs & derivatives* / Q000627:therapeutic use; D016158:Genes, p53; D015316:Genetic Therapy; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000628:therapy*; D009389:Neovascularization, Pathologic / Q000517:prevention & control; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
chemical_list: D016159:Tumor Suppressor Protein p53; D004958:Estradiol; D000077584:2-Methoxyestradiol