title: Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR.
abstract: Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR spectra show that aromatic carbons from the polystyrene core and oxygenated carbons in the poly(acrylic acid)-polyacrylamide shell are both proximate to the 19F of 6-fluorotryptophan. Molecular modeling restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptophans are in the shell but within 10 A of the core-shell interface.
authors.affiliation: Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Baugher
authors.forename: J M
authors.initials: JM
authors.lastname: Goetz
authors.forename: L M
authors.initials: LM
authors.lastname: McDowell
authors.forename: H
authors.initials: H
authors.lastname: Huang
authors.forename: K L
authors.initials: KL
authors.lastname: Wooley
authors.forename: J
authors.initials: J
authors.lastname: Schaefer
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018625:Microscopy, Atomic Force; D008863:Microspheres; D008968:Molecular Conformation; D015394:Molecular Structure; D011137:Polystyrenes / Q000737:chemistry; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D002247:Carbon Isotopes; D011137:Polystyrenes; D014364:Tryptophan; D000099166:Polyacrylamides; C001244:6-fluorotryptophan