title: Mechanisms of anterior cruciate ligament neovascularization and ligamentization.
abstract: Columbia-Rambouillet cross-bred sheep were used to study the revascularization and ligamentization process of anterior cruciate ligament (ACL) reconstruction over a 6-month period using basic histology, immunohistochemistry, and electron microscopy. The reconstruction technique studied was a quadruple-hamstring, interference screw fixation technique. Further, these specimens, after retrieval at 6, 12, and 26 weeks, were compared with human arthroscopic 'second looks' and with 10 en bloc specimens obtained when a cruciate-sacrificing total knee replacement was performed. The study showed that, with this reconstruction technique, Sharpey's fibers were seen at 6 weeks in both sheep and human specimens. The intratunnel specimens showed proliferative chondrification, then ossification of the matrix. Intra-articular neovascularization, ligamentization, and junction ossification occurred. Myoblasts or smooth muscle cells appear to mediate the ligamentization as evidenced in electron microscopy by proliferate collagen manufacture. These myoblasts were seen in both the healing sheep and human second looks, but not seen in mature ACL grafts or in normal ACLs. At 6 months postoperatively, the sheep ACL reconstruction appeared clinically, histologically, and immunohistochemically indistinguishable from the normal sheep ACL. A correlation of this work with published animal studies in which biomechanical testing was performed and with human 'second looks' would imply that an ACL reconstruction may be vulnerable during this period of neovascularization and ligamentization.
authors.affiliation: University of Washington, Seattle, USA.
authors.forename: P E
authors.initials: PE
authors.lastname: Scranton
authors.forename: W L
authors.initials: WL
authors.lastname: Lanzer
authors.forename: M S
authors.initials: MS
authors.lastname: Ferguson
authors.forename: T R
authors.initials: TR
authors.lastname: Kirkman
authors.forename: D S
authors.initials: DS
authors.lastname: Pflaster
mesh_terms: D000328:Adult; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000098:blood supply* / Q000473:pathology; D006614:Hindlimb; D006801:Humans; D007718:Knee Injuries / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D012756:Sheep