title: Endogenous proteolytic cleavage of normal and disease-associated isoforms of the human prion protein in neural and non-neural tissues.
abstract: We have investigated the proteolytic cleavage of the cellular (PrPC) and pathological (PrPSc) isoforms of the human prion protein (PrP) in normal and prion-affected brains and in tonsils and platelets from neurologically intact individuals. The various PrP species were resolved after deglycosylation according to their electrophoretic mobility, immunoreactivity, Sarkosyl solubility, and, as a novel approach, resistance to endogenous proteases. First, our data show that PrPC proteolysis in brain originates amino-truncated peptides of 21 to 22 and 18 (C1) kd that are similar in different regions and are not modified by the PrP codon 129 genotype, a polymorphism that affects the expression of prion disorders. Second, this proteolytic cleavage of PrPC in brain is blocked by inhibitors of metalloproteases. Third, differences in PrPC proteolysis, and probably in Asn glycosylation and glycosylphosphatidylinositol anchor composition, exist between neural and non-neural tissues. Fourth, protease-resistant PrPSc cores in sporadic Creutzfeldt-Jakob disease (CJD) and Gerstmann-Sträussler-Scheinker F198S disease brains all have an intact C1 cleavage site (Met111-His112), which precludes disruption of a domain associated with toxicity and fibrillogenesis. Fifth, the profile of endogenous proteolytic PrPSc peptides is characteristic of each disorder studied, thus permitting the molecular classification of these prion diseases without the use of proteinase K and even a recognition of PrPSc heterogeneity within type 2 CJD patients having different codon 129 genotype and neuropathological phenotype. This does not exclude the role of additional factors in phenotypic expression; in particular, differences in glycosylation that may be especially relevant in the new variant CJD. Proteolytic processing of PrP may play an important role in the neurotropism and phenotypic expression of prion diseases, but it does not appear to participate in disease susceptibility.
authors.affiliation: Department of Pathology, New York University Medical Center, New York 10016, USA.
authors.forename: A
authors.initials: A
authors.lastname: Jiménez-Huete
authors.forename: P M
authors.initials: PM
authors.lastname: Lievens
authors.forename: R
authors.initials: R
authors.lastname: Vidal
authors.forename: P
authors.initials: P
authors.lastname: Piccardo
authors.forename: B
authors.initials: B
authors.lastname: Ghetti
authors.forename: F
authors.initials: F
authors.lastname: Tagliavini
authors.forename: B
authors.initials: B
authors.lastname: Frangione
authors.forename: F
authors.initials: F
authors.lastname: Prelli
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000235:genetics / Q000473:pathology*; D001792:Blood Platelets / Q000737:chemistry; D001921:Brain / Q000473:pathology; D001923:Brain Chemistry; D003062:Codon; D007562:Creutzfeldt-Jakob Syndrome / Q000235:genetics / Q000473:pathology*; D005838:Genotype; D016098:Gerstmann-Straussler-Scheinker Disease / Q000235:genetics / Q000473:pathology*; D006031:Glycosylation; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism; D008875:Middle Aged; D009474:Neurons / Q000737:chemistry*; D014066:Palatine Tonsil / Q000737:chemistry; D010446:Peptide Fragments / Q000032:analysis; D010449:Peptide Mapping; D018621:PrPC Proteins / Q000032:analysis*; D018620:PrPSc Proteins / Q000032:analysis*
chemical_list: D003062:Codon; D010446:Peptide Fragments; D018621:PrPC Proteins; D018620:PrPSc Proteins; D008666:Metalloendopeptidases