title: Oxygen-free radicals and nitric oxide are involved in the thrombus growth produced by iontophoresis of ADP.
abstract: Thrombi have been induced by iontophoretic application of ADP on the venules of the mesentery of male Wistar rats (250-350 g). We determined the thrombus growth which is a reflection of platelet recruitment. We have demonstrated the ability of two oxygen-free radical scavengers, superoxide dismutase (SOD), a superoxide anion scavenger, and catalase, a hydrogen peroxide scavenger, to reduce thrombus growth. Imidazole, a thromboxane synthase inhibitor also reduces the thrombus growth. Dimethylthiourea, a hydroxyl radical scavenger, does not alter the thrombus size. The administration of a NO synthase inhibitor, Ng-nitro-L-arginine methyl ester (L-NAME), sharply increased the volume of the thrombus. Our results show the implication of superoxide anion, hydrogen peroxide and nitric oxide in platelet recruitment. (c) 1998 The Italian Pharmacological Society.
authors.affiliation: Unit of Pharmacology, Faculty of Medicine, University of Barcelona, Casanova 143, Barcelona, 08036, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Peire
authors.forename: P
authors.initials: P
authors.lastname: Puig-Parellada
mesh_terms: D000244:Adenosine Diphosphate / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000627:therapeutic use; D004791:Enzyme Inhibitors / Q000494:pharmacology; D066298:In Vitro Techniques; D007478:Iontophoresis; D008297:Male; D008642:Mesenteric Veins / Q000187:drug effects / Q000503:physiopathology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000502:physiology; D052250:Nitric Oxide Synthase Type III; D010974:Platelet Aggregation / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000502:physiology*; D013482:Superoxide Dismutase / Q000627:therapeutic use; D013927:Thrombosis / Q000188:drug therapy / Q000209:etiology*
chemical_list: D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D000244:Adenosine Diphosphate; D002374:Catalase; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat; D013482:Superoxide Dismutase; D019331:NG-Nitroarginine Methyl Ester