[Determination of puerarin in gegen ginlian tablets by RP-HPLC].
A quantitative method was developed for the determination of puerarin in Gegen Qinglian Tablets by reversed phase HPLC. Chromatographic conditions included column ODS-C18, column temperature: 35 C, UV detector: 250 nm, mobile phase: EtOH-H2O (23:77), flow rate: 1 ml/min. The number of theoretical plates calculated for puerarin peak was no less than 2000. The standard curve was linear in the concentration range of 5-80 micrograms/ml, and the correlation coefficient was 0.9999. The average recovery and the relative standard deviation were 97.6% and 1.8% respectivily.
Tan
S
S
Institute for Drug Control of Commission of Science, Technology and Industry for National Defence, Beijing.
Qiu
X
X
Li
G
G
Zhao
B
B
Liang
Y
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D007529:Isoflavones / Q000032:analysis*; D013607:Tablets
D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007529:Isoflavones; D013607:Tablets; C033607:puerarin