Synthesis of oligodeoxynucleotides containing N4-mercaptoethylcytosine and their use in the preparation of oligonucleotide-peptide conjugates carrying c-myc tag-sequence.
The preparation and properties of oligodeoxynucleotides containing mercaptoethyl groups at position N-4 of cytosine are described. The resulting thiol-oligodeoxynucleotides were reacted with a maleimido-peptide carrying the c-myc tag-sequence. The peptide-oligonucleotide conjugate is specifically recognized by an anti c-myc monoclonal antibody, thus constituting a labeling system with sensitivity similar to other existing methods of nonradioactive labeling.
Gottschling
D
D
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany, Universität Ulm, Sektion Polymere, Albert-Einstein-Allee 11, D-89081 Ulm, Germany.
Seliger
H
H
Tarrasón
G
G
Piulats
J
J
Eritja
R
R
D000911:Antibodies, Monoclonal; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D016259:Genes, myc / Q000235:genetics*; D007120:Immunochemistry; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D013696:Temperature
D000911:Antibodies, Monoclonal; D007202:Indicators and Reagents; C116455:N(4)-mercaptoethylcytosine; D009841:Oligonucleotides; D010455:Peptides; D003596:Cytosine