title: Cytotoxicity of three resin-based root canal sealers: an in vitro evaluation.
abstract: The cytotoxicity of three resin-based root canal sealers (AH26, AH-Plus, Topseal) was evaluated in vitro. The experiments included two cell lines, L929 mouse skin fibroblasts and RPC-C2A rat pulp cells. The cytotoxicity was assessed by sulforodamine B (SRB) colorimetric assay and hemocytometer viable cell counting after 24- and 48-h exposure. AH26 had a severe cytotoxic effect whilst Topseal and AH-Plus showed a markedly lower toxic influence on the cells during the experimental period.
authors.affiliation: Department of Endodontology, Dental School, Aristotle University, Thessaloniki, Greece.
authors.forename: E A
authors.initials: EA
authors.lastname: Koulaouzidou
authors.forename: K T
authors.initials: KT
authors.lastname: Papazisis
authors.forename: P
authors.initials: P
authors.lastname: Beltes
authors.forename: G D
authors.initials: GD
authors.lastname: Geromichalos
authors.forename: A H
authors.initials: AH
authors.lastname: Kortsaris
mesh_terms: D000818:Animals; D001729:Bismuth / Q000633:toxicity; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects*; D004338:Drug Combinations; D004853:Epoxy Resins / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects*; D007739:L Cells / Q000187:drug effects; D008709:Methenamine / Q000633:toxicity; D051379:Mice; D051381:Rats; D012387:Root Canal Filling Materials / Q000633:toxicity*; D012834:Silver / Q000633:toxicity; D014025:Titanium / Q000633:toxicity
chemical_list: D004338:Drug Combinations; D004853:Epoxy Resins; D012387:Root Canal Filling Materials; C115110:Topseal; C534916:epoxy resin-based root canal sealer; D012834:Silver; C028044:epoxy resin AH-26; D014025:Titanium; D008709:Methenamine; D001729:Bismuth