title: [Sensitivity and specificity of the PCR-test-system for diagnosing HIV infections].
abstract: Sensitivity and specificity of the Russian PCR test system (experimental lot) were assessed with materials from 107 Russian patients with HIV infection and 100 donors without specific antibodies to HIV-1. The electrophoretic and hybridization-enzymatic methods for amplicon detection were compared. The sensitivity of electrophoresis was 89.9%, specificity 91%; for hybridization-enzymatic detection these values were 96.1 and 100%, respectively. Samples which yielded false-negative results were repeatedly tested by two-staged amplification (nested PCR) with external and internal primers. The main cause of false-negative results was a low concentration of proviral HIV-1 DNA in some blood samples.
authors.forename: K A
authors.initials: KA
authors.lastname: Sarkisian
authors.forename: G A
authors.initials: GA
authors.lastname: Shipulin
authors.forename: M S
authors.initials: MS
authors.lastname: Vorob'eva
authors.forename: O Iu
authors.initials: OIu
authors.lastname: Shipulina
authors.forename: V I
authors.initials: VI
authors.lastname: Shakhgil'dian
authors.forename: A V
authors.initials: AV
authors.lastname: Kravchenko
authors.forename: E V
authors.initials: EV
authors.lastname: Buravtseva
authors.forename: L V
authors.initials: LV
authors.lastname: Serebrovskaia
authors.forename: V V
authors.initials: VV
authors.lastname: Pokrovskiĭ
authors.forename: E V
authors.initials: EV
authors.lastname: Korystova
mesh_terms: D015492:AIDS Serodiagnosis / Q000592:standards*; D004279:DNA, Viral / Q000097:blood; D005188:False Negative Reactions; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000592:standards*; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral