[A method of isolating the main outer membrane protein of Chlamydia].
Purified chlamydial bodies were solubilized by detergent solutions used in the following sequence: 1) 1% sarcosil, 2) 1% sarcosil + 10 mM dithiotreitol, 3) 2% sodium dodecyl sulfate + 10 mM dithiotreitol. After the third stage a good yield of protein, corresponding to major outer membrane protein as to its molecular weight and antigenic properties was obtained.
Bilozorov
O P
OP
Ukrainian Research Institute of Dermatology and Venerology, Ministry of Health of Ukraine, Kharkiv.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001425:Bacterial Outer Membrane Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D002642:Chick Embryo; D002692:Chlamydia trachomatis / Q000276:immunology; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000302:isolation & purification; D007114:Immunization; D007124:Immunoenzyme Techniques; D011817:Rabbits; D012995:Solubility
D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D003470:Culture Media; D007106:Immune Sera