title: Streptococcal emm types associated with T-agglutination types and the use of conserved emm gene restriction fragment patterns for subtyping group A streptococci.
abstract: The T-agglutination types were determined for a diverse collection of 1531 group A streptococci for which the 5' M protein gene (emm) sequences had been analysed. The majority of the T-agglutination types correlated with previously seen M/emm/T-type associations; however, several new associations were found. Analysis of a subset of this collection -- which included 1157 clinical isolates with multiply encountered emm types -- found that emm amplicon restriction profiles of isolates sharing identical T types and opacity factor phenotypes are useful for detecting groups of isolates with identical emm genes. Many emm genes of known 5' sequence display a highly conserved restriction pattern amongst clinical isolates widely separated both geographically and temporally.
authors.affiliation: Centers for Disease Control and Prevention, Childhood and Respiratory Infections, Atlanta, GA 30333, USA.
authors.forename: B
authors.initials: B
authors.lastname: Beall
authors.forename: R R
authors.initials: RR
authors.lastname: Facklam
authors.forename: J A
authors.initials: JA
authors.lastname: Elliott
authors.forename: A R
authors.initials: AR
authors.lastname: Franklin
authors.forename: T
authors.initials: T
authors.lastname: Hoenes
authors.forename: D
authors.initials: D
authors.lastname: Jackson
authors.forename: L
authors.initials: L
authors.lastname: Laclaire
authors.forename: T
authors.initials: T
authors.lastname: Thompson
authors.forename: R
authors.initials: R
authors.lastname: Viswanathan
mesh_terms: D000371:Agglutination; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002352:Carrier Proteins; D017124:Conserved Sequence; D006801:Humans; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D013297:Streptococcus pyogenes / Q000145:classification* / Q000235:genetics; D014481:United States
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C021908:opacity factor; C014689:streptococcal M protein; D010447:Peptide Hydrolases