title: pH sensitive properties of Tc(V)-DMS: analytical and in vitro cellular studies.
abstract: Numerous clinical studies with the pentavalent technetium complex of dimercaptosuccinic acid [Tc(V)-DMS] seem to indicate its new role in nuclear oncology. Thus, we questioned what properties of the Tc(V)-DMS molecule associate with its tumoral tissue accumulation. Because studies have reported tumor tissue to be more acidic than normal tissue, acidification might be related to the Tc(V)-DMS localization in tumor tissue. Thus, in the present study, a working hypothesis drew to test the acidification as a plausible factor, and various analytical methods and an in vitro cellular system using Ehrlich ascites tumor cells (EATC) implemented. Analytical methodologies demonstrated the decrease of the overall negative charge of the Tc(V)-DMS molecule, promoted by the acidification of the analytical medium and the sample dilution. In the in vitro cellular experiment, acidification alone showed no effect on the radioactivity accumulation in EATC; nevertheless, if accompanied by a pre-dilution of the Tc(V)-DMS sample added into the cell incubation media, cellular radioactivity accumulation was observed. Thus, acidification as a mediator for the Tc(V)-DMS accumulation in tumoral cells, concurrently with dilution as the promoter of the process, constituted the foundation for discerning the working hypothesis.
authors.affiliation: Department of Patho-Functional Bioanalysis, Faculty of Pharmaceutical Sciences, Kyoto University, Japan. shkazuko@pharm.kyoto-u.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Horiuchi
authors.forename: H
authors.initials: H
authors.lastname: Saji
authors.forename: A
authors.initials: A
authors.lastname: Yokoyama
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000143:Acids / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002286:Carcinoma, Ehrlich Tumor / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008956:Models, Chemical; D009374:Neoplasms, Experimental; D019783:Technetium Tc 99m Dimercaptosuccinic Acid / Q000737:chemistry* / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000143:Acids; D008024:Ligands; D019783:Technetium Tc 99m Dimercaptosuccinic Acid; D019342:Acetic Acid