Lack of association between osteoarthritis of the hip and gene polymorphisms of VDR, COL1A1, and COL2A1 in postmenopausal women.
OBJECTIVE
To evaluate whether DNA polymorphisms of the VDR (vitamin D receptor), COL1A1 (alpha1 type I collagen), and COL2A1 (alpha1 type II collagen) genes, which have previously been linked to bone mineral density (BMD) and/or osteoarthritis (OA), are also associated with OA of the hip (OAH).


METHODS
Genotype frequency distributions were compared between a group of 239 elderly healthy female controls and a group of 75 female OAH patients who underwent total hip replacement. All participants underwent BMD measurements of the total body, lumbar spine, and femoral neck. Mutation detection was done using polymerase chain reaction followed by restriction enzyme digestion. Polymorphisms were detected by digestion with Bsm I for VDR, Acc B7I for COL1A1, and Pvu II for COL2A1.


RESULTS
None of the genotype frequency distributions of any of the examined polymorphisms was significantly different between the OAH group and the control group. No significant differences in BMD variables were observed after stratification of the subjects according to the examined genotypes within each study group.


CONCLUSIONS
In Belgian postmenopausal women, the examined polymorphisms of the candidate genes VDR, COL1A1, and COL2A1 do not significantly contribute to an increased prevalence of OAH or to differences in BMD.
Aerssens
J
J
Arthritis and Metabolic Bone Disease Research Unit, Leuven, Belgium.
Dequeker
J
J
Peeters
J
J
Breemans
S
S
Boonen
S
S
D000368:Aged; D000369:Aged, 80 and over; D001530:Belgium; D003094:Collagen / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D015207:Osteoarthritis, Hip / Q000235:genetics*; D011110:Polymorphism, Genetic; D017698:Postmenopause; D011347:Procollagen / Q000235:genetics*; D018167:Receptors, Calcitriol / Q000235:genetics*; D015533:Transcriptional Activation
D011347:Procollagen; D018167:Receptors, Calcitriol; D003094:Collagen