title: Identification and characterisation of serogroup M among Nepalese isolates of Dichelobacter nodosus, the transmitting agent of footrot in small ruminants.
abstract: One thousand and sixty three isolates of Dichelobacter nodosus cultured between 1992 and 1996 from cases of footrot in sheep and goats of migratory flocks of Nepal were characterised by agglutination test using prototype antisera of the Australian classification system. Of those, sixty six isolates could not be classified into any of the nine serogroups (A-I). This study was therefore undertaken to characterise these isolates. It was established that they were agglutinated by antiserum against serotype M of an alternative classification system. The distinct antigenic character of these isolates was further confirmed by DNA sequence analysis of the gene for the fimbrial subunit protein of two of them. At a molecular level, these isolates were closer to the prototype of serogroup F, VCS 1017. However, when compared with VCS 1017, the number of amino acid substitutions (28) in the fimbrial protein of these isolates was similar to that expected between isolates of different serogroups. Because these isolates are antigenically similar to 'serotype' M, but meet all the criteria to be classified into an independent serogroup, it is proposed that these isolates together with isolates previously classified as serotype M be classified as 'serogroup M'.
authors.affiliation: Lumle Agricultural Research Centre, Pokhara, Nepal.
authors.forename: S C
authors.initials: SC
authors.lastname: Ghimire
authors.forename: J R
authors.initials: JR
authors.lastname: Egerton
authors.forename: O P
authors.initials: OP
authors.lastname: Dhungyel
authors.forename: H D
authors.initials: HD
authors.lastname: Joshi
mesh_terms: D000372:Agglutination Tests; D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics; D001483:Base Sequence; D020376:Dichelobacter nodosus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010861:Fimbriae, Bacterial / Q000235:genetics; D005535:Foot Rot / Q000382:microbiology*; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009390:Nepal; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012418:Ruminants; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S