The detection by enzyme linked immunosorbent assay of P30 and 19-OH prostaglandin F1/F2, in the presence of a range of possible contaminants.
The potential of specific human semen markers, P30 and 19-OH prostaglandin F1 alpha/F2 alpha (PG F), ELISAs were compared in a blind trial. The PG F assay was shown to be at least ten times more sensitive than the P30 technique although cross reactivity problems with spermicidal creams and other possible domestic contaminants were identified. A simple method for the removal of such contaminants using a phenyl boronate column (PBA) was evaluated in an extensive blind trial.
Sutton
J G
JG
Forensic Science Service, London Laboratory, United Kingdom.
Bosley
C
C
Rands
A
A
D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005554:Forensic Medicine; D006801:Humans; D008297:Male; D017430:Prostate-Specific Antigen / Q000032:analysis; D029584:Prostatic Secretory Proteins; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins; D012742:Sex Offenses
D029584:Prostatic Secretory Proteins; D011506:Proteins; D029589:Seminal Plasma Proteins; C043076:beta-microseminoprotein; C025183:19-hydroxyprostaglandin F; D015237:Dinoprost; D017430:Prostate-Specific Antigen