title: Solution structure of a protein inhibitor of neuronal nitric oxide synthase.
abstract: The structure of the neuronal nitric oxide synthase inhibitory protein, PIN (protein inhibitor of nNOS), has been determined by NMR spectroscopy. Two N-terminal antiparallel alpha-helices pack against a four-stranded antiparallel beta-sheet in the C-terminal region of the protein, forming a two-layer alpha/beta plait. The three dimensional structure of PIN resembles the fold of the B-chain of aspartylglucosaminidase. A non-prolyl cis peptide bond was found between Pro 52 and Thr 53 of the protein. PIN has a large solvent-exposed hydrophobic surface that contains a cavity and is rimmed with positive charges. This surface may serve as the primary target-binding region for this multi-functional regulatory protein.
authors.affiliation: Department of Biochemistry, The Hong Kong University of Science and Technology, Kowloon, P.R. China.
authors.forename: H
authors.initials: H
authors.lastname: Tochio
authors.forename: S
authors.initials: S
authors.lastname: Ohki
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: M
authors.initials: M
authors.lastname: Li
authors.forename: M
authors.initials: M
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001227:Aspartylglucosylaminase / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry*; D018360:Crystallography, X-Ray; D029721:Drosophila Proteins; D004398:Dyneins; D004791:Enzyme Inhibitors / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D052248:Nitric Oxide Synthase Type I; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D051381:Rats
chemical_list: D002352:Carrier Proteins; D029721:Drosophila Proteins; D004791:Enzyme Inhibitors; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat; D001227:Aspartylglucosylaminase; D004398:Dyneins