title: [Effect of adenosine on intracellular free calcium in cultured rat hippocampal CA1 neurons during anoxia].
abstract: Intracellular free Ca2+ concentration ([Ca2+]i) was measured by laser scanning confocal microscope, using Ca2+ indicator Fluo-3 in cultured hippocampal CA1 neurons isolated from newborn rat. The results showed that acute anoxia induced a rapid increase of [Ca2+]i in hippocampal CA1 neurons, and this increase could be attenuated by 100 mumol/L adenosine significantly. This effect of adenosine could be suppressed by adenosine A1 receptor antagonist CPT or potassium channel blockers, 4-AP and glipizide. These results suggest that adenosine activates 4-AP- or ATP-sensitive potassium channels through A1 receptors, and consequently inhibits the [Ca2+]i elevation in hippocampal neurons during anoxia.
authors.affiliation: Institute of Basic Medical Sciences, Beijing.
authors.forename: Q
authors.initials: Q
authors.lastname: Wan
authors.forename: F Z
authors.initials: FZ
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Yao
authors.forename: Z W
authors.initials: ZW
authors.lastname: Liu
authors.forename: Y H
authors.initials: YH
authors.lastname: Huang
authors.forename: A S
authors.initials: AS
authors.lastname: Ding
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018613:Microscopy, Confocal; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000241:Adenosine; D002118:Calcium