title: Hepatocellular carcinomas infected with the novel TT DNA virus lack viral integration.
abstract: A novel DNA virus designated TT virus (TTV) was cloned from a patient with posttransfusion hepatitis and is thought to be a new hepatitis virus. At present, hepatitis B virus (HBV) and hepatitis C virus (HCV) are known to induce hepatocellular carcinoma (HCC). But, actually, in Japan approximately 5 to 10% of HCCs are in HBV-negative and HCV-negative (NBNC) patients. In order to study the possible role of TTV in hepatocarcinogenesis, we investigated the frequency of the TTV genome in liver tissue of 20 HCC patients. As a result, 3 of 8 NBNC HCC patients and 5 of 12 HBV- or HCV-associated HCC patients were TTV positive, and TTV was shown not to be specific for NBNC HCC. For all TTV-positive patients, we also confirmed that the TTV genome was not integrated into host hepatocyte DNA.
authors.affiliation: Department of Experimental Pathology, Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo, 170-8455, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: K
authors.initials: K
authors.lastname: Kajino
authors.forename: M
authors.initials: M
authors.lastname: Ogawa
authors.forename: I
authors.initials: I
authors.lastname: Gotoh
authors.forename: S
authors.initials: S
authors.lastname: Matsuoka
authors.forename: K
authors.initials: K
authors.lastname: Suzuki
authors.forename: M
authors.initials: M
authors.lastname: Moriyama
authors.forename: H
authors.initials: H
authors.lastname: Okubo
authors.forename: M
authors.initials: M
authors.lastname: Kudo
authors.forename: Y
authors.initials: Y
authors.lastname: Arakawa
authors.forename: O
authors.initials: O
authors.lastname: Hino
mesh_terms: D000368:Aged; D015139:Blotting, Southern; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000821:virology*; D004266:DNA Virus Infections / Q000235:genetics / Q000821:virology; D004267:DNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D006518:Hepatitis Viruses / Q000235:genetics / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000235:genetics / Q000821:virology; D006801:Humans; D008099:Liver / Q000737:chemistry / Q000821:virology; D008113:Liver Neoplasms / Q000235:genetics / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D016662:Virus Integration
chemical_list: D004279:DNA, Viral