Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: an assessment using mutations in yeast cytochrome c oxidase subunits I and II.
We have examined deficiency mutations and reversions in subunits I and II of yeast cytochrome c oxidase in order to test the reliability of second-site reversion analysis in prediction of tertiary structure of a membrane protein complex. It appears that the method can not provide information on distance between residues, since reversions can be up to 30 A from the primary mutations. However, the reversions are not randomly located in the structure but reveal regions essential for assembly or functional units.
Meunier
B
B
Department of Biology, University College London, Gower Street, London, WC1E 6BT, UK. b.meunier@ucl.ac.uk
Rich
P R
PR
D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV / Q000737:chemistry* / Q000235:genetics; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D010230:Paracoccus / Q000201:enzymology; D011487:Protein Conformation
D005656:Fungal Proteins; D008565:Membrane Proteins; D003576:Electron Transport Complex IV