title: [Boar semen--a possible risk factor in infection occurrence of porcine reproductive and respiratory syndrome].
abstract: Based on an experimental study using two and six PRRS-negative young boars and gilts, respectively, it was proven wether boar semen could be a risk factor in the transmission of the disease. The two boars were inoculated intranasally with the PRRS-virus strain I10 (Intervet) containing 107 TCID50/ml. Using ovulation synchronization six gilts were prepared as bioindicators to be inseminated, two of them with semen of the two boars at day 4, 8 and 12 after infection. Following inoculation of the boars, PRRS virus was shown to be present in blood from the 2nd until the 35th and 40th day p.i., respectively. PRRS virus could also be isolated from nasal swabs at day 6, 9, 12 and 19 and from preputial swabs at day 4, 12 and 27 after infection. PRRS virus could only be detected at day 19 p.i. in semen of one boar. Drastic changes in quality and volume of the ejaculate were observed about day 25 p.i. in both animals. Insemination of the gilts with semen collected at day 4, 8 and 12 p.i., however, did not lead to an infection of the females, because neither clinical signs typical for PRRS nor seroconversion could be observed. Reproductive parameters as well as birth and growing traits of the gilts were in accordance with norm values.
authors.affiliation: Institut für Epidemiologie, Bundesforschungsanstalt für Viruskrankheiten der Tiere.
authors.forename: J
authors.initials: J
authors.lastname: Teuffert
authors.forename: H
authors.initials: H
authors.lastname: Schlüter
authors.forename: T
authors.initials: T
authors.lastname: Müller
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D005260:Female; D008297:Male; D009297:Nasal Mucosa / Q000821:virology; D010413:Penis / Q000821:virology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000209:etiology* / Q000635:transmission; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology / Q000302:isolation & purification*; D012307:Risk Factors; D012661:Semen / Q000166:cytology / Q000821:virology*; D013552:Swine; D014766:Viremia / Q000662:veterinary / Q000821:virology
chemical_list: D000914:Antibodies, Viral