title: A comparison of LDL size determination using gradient gel electrophoresis and light-scattering methods.
abstract: This study compared gradient gel electrophoresis (GGE) and light-scattering (LS) methods of determining low density lipoprotein (LDL) particle size. LDL was isolated from 27 fasting subjects. Peak particle size was determined by GGE on 3-13% gradient gels (Gradipore, Sydney, Australia) and by LS using a Zetasizer 3000 (Malvern Instruments, Malvern, UK). Repeated measurements on a single specimen indicated a coefficient of variation (CV) of 0.3%. A correlation was noted (P < 0.0001; r = 0.78) when comparing LDL particle size determined by LS methodology and GGE. Particle diameter results obtained by LS were smaller than those obtained by GGE (23.1 +/- 0.1 vs. 26.1 +/- 0.1 nm; P < 0.0001). LDL particle size determined by LS methodology correlated inversely with the log of triglyceride level (P < 0.0001; r = -0.77) and positively with high density lipoprotein (HDL) cholesterol level (P < 0.002; r = 0.57).
authors.affiliation: Department of Medicine, University of Melbourne, St. Vincent's Hospital, Fitzroy, Victoria, Australia.
authors.forename: D
authors.initials: D
authors.lastname: O'Neal
authors.forename: P
authors.initials: P
authors.lastname: Harrip
authors.forename: G
authors.initials: G
authors.lastname: Dragicevic
authors.forename: D
authors.initials: D
authors.lastname: Rae
authors.forename: J D
authors.initials: JD
authors.lastname: Best
mesh_terms: D008076:Cholesterol, HDL / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005260:Female; D006801:Humans; D008027:Light; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010316:Particle Size; D012542:Scattering, Radiation; D014280:Triglycerides / Q000097:blood
chemical_list: D008076:Cholesterol, HDL; D008077:Lipoproteins, LDL; D014280:Triglycerides