Location of fluorotryptophan sequestered in an amphiphilic nanoparticle by rotational-echo double-resonance NMR.
Rotational-echo double-resonance (REDOR) 13C NMR spectra (with 19F dephasing) have been obtained of 6-fluorotryptophan complexed by a polymeric amphiphilic nanosphere consisting of a polystyrene core covalently attached to a poly(acrylic acid)-polyacrylamide shell. The REDOR spectra show that aromatic carbons from the polystyrene core and oxygenated carbons in the poly(acrylic acid)-polyacrylamide shell are both proximate to the 19F of 6-fluorotryptophan. Molecular modeling restrained by distances inferred from the REDOR spectra suggests that all of the 6-fluorotryptophans are in the shell but within 10 A of the core-shell interface.
Baugher
A H
AH
Department of Chemistry, Washington University, St. Louis, Missouri 63130, USA.
Goetz
J M
JM
McDowell
L M
LM
Huang
H
H
Wooley
K L
KL
Schaefer
J
J
D000180:Acrylic Resins / Q000737:chemistry; D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D018625:Microscopy, Atomic Force; D008863:Microspheres; D008968:Molecular Conformation; D015394:Molecular Structure; D011137:Polystyrenes / Q000737:chemistry; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry; D000099166:Polyacrylamides
D000180:Acrylic Resins; D002247:Carbon Isotopes; D011137:Polystyrenes; D014364:Tryptophan; D000099166:Polyacrylamides; C001244:6-fluorotryptophan