title: Protective role of vagal afferents in experimentally-induced colitis in rats.
abstract: The aim of this study was to evaluate the regulatory role of vagal afferents in the development of colonic inflammation induced by trinitrobenzenesulfonic acid (TNBS) in rats. Groups of Wistar rats were treated with capsaicin or its vehicle applied perivagally (sham treatment). Colonic transit time was evaluated, and, two days later, one half of the animals received an intracolonic instillation of TNBS/ethanol (40 mg/kg), and the other received saline. Inflammation was evaluated functionally (gut permeability), biochemically (myeloperoxydase activity) and histologically. Vagal capsaicin deafferentation did not modify colonic transit time. In TNBS treated groups, inflammation was enhanced by capsaicin pretreatment, as determined by an increased gut permeability, MPO activity, and histological damage score. These results suggest that vagal afferents have a protective role in TNBS-induced colitis in rats, unrelated to changes in colonic transit time.
authors.affiliation: Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
authors.forename: L
authors.initials: L
authors.lastname: Mazelin
authors.forename: V
authors.initials: V
authors.lastname: Theodorou
authors.forename: J
authors.initials: J
authors.lastname: More
authors.forename: J
authors.initials: J
authors.lastname: Fioramonti
authors.forename: L
authors.initials: L
authors.lastname: Bueno
mesh_terms: D000818:Animals; D001341:Autonomic Nervous System / Q000201:enzymology / Q000276:immunology; D002211:Capsaicin / Q000494:pharmacology; D003092:Colitis / Q000139:chemically induced / Q000276:immunology*; D003106:Colon / Q000276:immunology / Q000294:innervation; D003714:Denervation; D007408:Intestinal Absorption / Q000502:physiology; D007413:Intestinal Mucosa / Q000276:immunology / Q000294:innervation / Q000378:metabolism; D008297:Male; D009475:Neurons, Afferent / Q000502:physiology*; D009504:Neutrophils / Q000201:enzymology; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014302:Trinitrobenzenesulfonic Acid; D014630:Vagus Nerve / Q000166:cytology* / Q000276:immunology
chemical_list: D014302:Trinitrobenzenesulfonic Acid; D009195:Peroxidase; D002211:Capsaicin