TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation.
Regulation of cell adhesion is important for immune system function. CD44 is a tightly regulated cell adhesion molecule present on leukocytes and implicated in their attachment to endothelium during an inflammatory immune response. The proinflammatory cytokine tumor necrosis factor-alpha, but not interferon-gamma, was found to convert CD44 from its inactive, nonbinding form to its active form by inducing the sulfation of CD44. This posttranslational modification was required for CD44-mediated binding to the extracellular matrix component hyaluronan and to vascular endothelial cells. Sulfation is thus a potential means of regulating CD44-mediated leukocyte adhesion at inflammatory sites.
Maiti
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Department of Microbiology and Immunology, University of British Columbia, 300-6174 University Boulevard, Vancouver, B.C. V6T 1Z3, Canada.
Maki
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Johnson
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D002448:Cell Adhesion; D002704:Chlorates / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000502:physiology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism* / Q000502:physiology; D006820:Hyaluronic Acid / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology; D007962:Leukocytes / Q000502:physiology*; D011499:Protein Processing, Post-Translational; D013431:Sulfates / Q000378:metabolism*; D015238:Sulfotransferases / Q000037:antagonists & inhibitors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*
D002704:Chlorates; D004791:Enzyme Inhibitors; D018960:Hyaluronan Receptors; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D006820:Hyaluronic Acid; D015238:Sulfotransferases; C032706:sodium chlorate