title: Determination of the yield of radiation-induced peroxidation of sodium linoleate in aqueous monomeric and micellar solutions.
abstract: Peroxidation of polyunsaturated fatty acids such as linoleic acid in aqueous micellar solution proceeds through a free-radical chain mechanism and is accompanied by the formation of conjugated dienes, some in the form of hydroperoxides. In the course of an investigation of radiation-induced oxidation of aqueous sodium linoleate, we have measured three indexes of peroxidation-conjugated dienes, hydroperoxides and thiobarbituric acid-reactive substances-by means of absorption spectroscopy, high-pressure liquid chromatography and spectrofluorimetry, respectively. There are linear correlations between the amounts of conjugated dienes, hydroperoxides and thiobarbituric acid-reactive substances. The radiolytic yields have been determined from the radiation dose dependence of the three markers of peroxidation as a function of sodium linoleate concentration. The results obtained indicate a strong effect of the concentrations of oxygen and linoleate on the yields of the products. The yields at different lipid concentrations display a large increase in chain propagation length; this is discussed in terms of the effect of micellar size.
authors.affiliation: Laboratoire de Chimie-Physique, URA 400 CNRS, Université Paris V, France.
authors.forename: C
authors.initials: C
authors.lastname: Hauville
authors.forename: S
authors.initials: S
authors.lastname: Rémita
authors.forename: P
authors.initials: P
authors.lastname: Thérond
authors.forename: A
authors.initials: A
authors.lastname: Rouscilles
authors.forename: M
authors.initials: M
authors.lastname: Couturier
authors.forename: D
authors.initials: D
authors.lastname: Jore
authors.forename: M
authors.initials: M
authors.lastname: Gardès-Albert
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004307:Dose-Response Relationship, Radiation; D019787:Linoleic Acid / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000528:radiation effects*; D008823:Micelles; D012996:Solutions; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D008823:Micelles; D012996:Solutions; D017392:Thiobarbituric Acid Reactive Substances; D019787:Linoleic Acid