Real-time patch-cram detection of intracellular cGMP reveals long-term suppression of responses to NO and muscarinic agonists.
Cyclic GMP (cGMP) is a crucial intracellular messenger in neuronal, muscle, and endocrine cells. The intracellular concentration of cGMP is regulated by various neurotransmitters, including acetylcholine (ACh) and nitric oxide (NO). While much is known about the biochemical steps leading to cGMP synthesis, little is known about cGMP kinetics in intact cells. Here, we use "patch-cramming," in which an excised, inside-out membrane patch containing cyclic nucleotide-gated ion channels is used as a biosensor, to obtain the first real-time measurements of cGMP in intact cells. Patch-cramming experiments on neuroblastoma cells show that both muscarinic agonists and NO rapidly elevate cGMP. NO elicits cGMP responses repeatedly without decrement, whereas responses to muscarinic agonists exhibit a profound and prolonged desensitization. Remarkably, muscarinic agonists also cause long-term (>30 min) suppression (LTS) of cGMP responses elicited by NO. Biochemical measurements reveal that rat sympathetic neurons also exhibit LTS of cGMP, suggesting that LTS is a widespread mechanism that may contribute to synaptic plasticity.
Trivedi
B
B
Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Florida 33101, USA.
Kramer
R H
RH
D000818:Animals; D003199:Computer Systems; D006152:Cyclic GMP / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004594:Electrophysiology / Q000379:methods; D007425:Intracellular Membranes / Q000378:metabolism*; D007700:Kinetics; D008046:Lip / Q000502:physiology*; D018721:Muscarinic Agonists / Q000494:pharmacology*; D009569:Nitric Oxide / Q000494:pharmacology*; D009865:Oocytes / Q000378:metabolism; D051381:Rats; D014407:Tumor Cells, Cultured; D014981:Xenopus
D018721:Muscarinic Agonists; D009569:Nitric Oxide; D006152:Cyclic GMP