title: Phosphorylation of LukS by protein kinase A is crucial for the LukS-specific function of the staphylococcal leukocidin on human polymorphonuclear leukocytes.
abstract: Staphylococcal leukocidin (Luk) consists of two protein components, LukF and LukS, which cooperatively lyse human and rabbit polymorphonuclear leukocytes. Here, we demonstrate that the phosphorylation of LukS by protein kinase A is crucial for the LukS-specific leukocytolytic function of Luk on HPMNLs by using N-[2(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89), which is a potent and selective inhibitor of protein kinase A. At 0.5 microM H-89 completely prevented the Luk-induced cell lysis accompanied by blocking of the incorporation of exogenous 32P-H3PO4 into LukS on HPMNLs. However, with LukS and LukF together, 0.5 microM H-89 did not inhibit the cell swelling which takes place before the cell lysis. HPMNLs also became swollen upon treating with both LukF and LukS mutants which could not be phosphorylated.
authors.affiliation: Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Nishiyama
authors.forename: H
authors.initials: H
authors.lastname: Nariya
authors.forename: Y
authors.initials: Y
authors.lastname: Kamio
mesh_terms: D001426:Bacterial Proteins; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D006801:Humans; D007956:Leukocidins / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism*; D010766:Phosphorylation; D013211:Staphylococcus aureus / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D007956:Leukocidins; C108483:leukocidin S-component protein, Staphylococcus; D017868:Cyclic AMP-Dependent Protein Kinases