[Studies on biological effects of kappa-selenocarrageenan on human breast cancer cell line BCaP-37].
Proliferation, cell cycle, total amount of DNA, area of cell nucleus, as well as epidermal growth factor receptors (EGFR) and expression of oncogene C-erbB2 mRNA of Chinese breast cancer cell line (BCaP-37) after being treated with kappa-selenocarrageenan were determined by cell culture technique, image cytometry (ICM) and northern blot to explore its anti-tumor mechanism. Results revealed 3.0-120 mg/L selenocarrageenan could inhibit proliferation of BCaP-37, with a response of time and dose dependence. The areas of nuclei were significantly lower with ICM in cells treated with 15 or 60 mg/L selenocarrageenan for four days than those in controls (P < 0.01). Levels of EGFR and expression of C-erbB2 mRNA were significantly inhibited in cells treated with 60 mg/L selenocarrageenan. It suggests that selenocarrageenan can inhibit proliferation of breast cancer cells through regulation of the levels of EGFR and expression of C-erbB2 mRNA.
He
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Department of Nutrition and Food Hygiene, Shanghai Medical University.
Cheng
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C
Lu
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D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000473:pathology*; D018276:Carcinoma, Medullary / Q000473:pathology*; D002351:Carrageenan / Q000494:pharmacology*; D066246:ErbB Receptors / Q000096:biosynthesis; D005260:Female; D006801:Humans; D016566:Organoselenium Compounds / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D002351:Carrageenan; D066246:ErbB Receptors; D016566:Organoselenium Compounds; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C079969:kappa-selenocarrageenan