Low oxygen tension induces positive inotropy and decreases a(i)Na in isolated guinea-pig cardiac ventricular papillary muscles.
Effects of low oxygen on contractile force, intracellular Na+ activity (aiNa), and action potential were simultaneously measured in isolated guinea-pig ventricular papillary muscles. Reduction of oxygen from control 488 to 150 mmHg biphasically increased and decreased the twitch tension, and decreased aiNa in muscles driven at 60 beats/min. The action potential duration (APD) was decreased but the maximum rate of upstroke (Vmax) was increased. In control, 1 microM epinephrine significantly increased the the action potential amplitude and twitch tension with decreases in the time to twitch peak (TTP), time for 50% relaxation (RT50), and aiNa. After exposure to low oxygen for 10 min, with twitch tension elevated and TTP and RT90 increased, 1 microM epinephrine significantly increased the twitch tension and Vmax, and decreased the APD and aiNa. Pretreatment with reserpine inhibited the twitch tension, both at control and in the presence of epinephrine. But changes of action potential and aiNa in response to low oxygen and epinephrine were similar to those in control. Our results indicate that the isolated guinea-pig ventricular muscle needs a high oxygen tension to maintain a normal contractile function. Reduction of oxygen deteriorates the electrical and mechanical activities, most likely, by a coaxial graded hypoxia. The decreased aiNa, not associated with endogenous catecholamines, suggests that the activity of the Na(+)-K+ pump can be maintained in the superficial muscle cells despite of core-central hypoxia.
Jao
M J
MJ
Department of Physiology and Biophysics, National Defense Medical Center, Taipei, Taiwan, ROC.
Yang
J M
JM
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D004837:Epinephrine / Q000494:pharmacology; D006168:Guinea Pigs; D006352:Heart Ventricles / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D007424:Intracellular Fluid / Q000378:metabolism; D009200:Myocardial Contraction / Q000502:physiology*; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D010100:Oxygen / Q000502:physiology*; D010210:Papillary Muscles / Q000166:cytology / Q000378:metabolism; D010313:Partial Pressure; D012110:Reserpine / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*; D013565:Sympatholytics / Q000494:pharmacology; D013566:Sympathomimetics / Q000494:pharmacology; D014662:Vasoconstrictor Agents / Q000494:pharmacology
D013565:Sympatholytics; D013566:Sympathomimetics; D014662:Vasoconstrictor Agents; D012110:Reserpine; D012964:Sodium; D010100:Oxygen; D004837:Epinephrine