title: Interaction of Theiler's virus with intermediate filaments of infected cells.
abstract: Theiler's murine encephalomyelitis virus is a neurotropic murine picornavirus which replicates permissively and causes a cytopathic effect in the BHK-21 cell line. We examined the interactions between the GDVII and DA strains of Theiler's virus and BHK-21 host cell proteins in a virus overlay assay. We observed binding of the virions to two proteins of approximately 60 kDa. These proteins were microsequenced and identified as desmin and vimentin, two main components of the intermediate filament network. The association between desmin or vimentin and virions was demonstrated by immunoprecipitation. Anti-desmin and anti-vimentin monoclonal antibodies precipitated GDVII or DA virions from extracts of infected BHK-21 cells. The intracellular distributions of virions and of the desmin and vimentin intermediate filaments of BHK-21 cells were investigated by two-color immunofluorescence confocal microscopy. Following infection, the intermediate filament network was rearranged into a shell-like structure which surrounded a viral inclusion. Finally, close contact between GDVII virus particles and 10-nm intermediate filaments was observed by electron microscopy.
authors.affiliation: Unité des Virus Lents (ERS 572 CNRS), Département SIDA & Rétrovirus, Institut Pasteur, 75724 Paris Cedex 15, France.
authors.forename: P
authors.initials: P
authors.lastname: Nédellec
authors.forename: P
authors.initials: P
authors.lastname: Vicart
authors.forename: C
authors.initials: C
authors.lastname: Laurent-Winter
authors.forename: C
authors.initials: C
authors.lastname: Martinat
authors.forename: M C
authors.initials: MC
authors.lastname: Prévost
authors.forename: M
authors.initials: M
authors.lastname: Brahic
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003893:Desmin / Q000378:metabolism; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D007382:Intermediate Filaments / Q000378:metabolism / Q000821:virology*; D051379:Mice; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D017945:Theilovirus / Q000254:growth & development / Q000472:pathogenicity* / Q000502:physiology; D014746:Vimentin / Q000378:metabolism
chemical_list: D003893:Desmin; D014746:Vimentin