Type 1 Cu structure of blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 at 2.05 A resolution: comparison of blue and green nitrite reductases.
The crystal structure of the blue nitrite reductase from Alcaligenes xylosoxidans GIFU 1051 (AxgNIR) has been determined at 2.05 A resolution. AxgNIR contains both type 1 and 2 Cu sites, the geometry of the former being distorted tetrahedral. The superpositioning of the type 1 Cu sites in the blue enzyme and a green nitrite reductase revealed that the orientation of the Met150 side chain differed. The deviation of the Sdelta(Met150) atom from the axial position of the NNS plane formed by two Ndelta(His95 and His145) and one Sgamma(Cys136) atom caused the difference in the colors of the enzymes, i.e. blue and green.
Inoue
T
T
Department of Materials Chemistry, Graduate School of Engineering, Osaka University, Suita, Osaka, 565-0871, Japan.
Gotowda
M
M
Deligeer
Kataoka
K
K
Yamaguchi
K
K
Suzuki
S
S
Watanabe
H
H
Gohow
M
M
Kai
Y
Y
D000421:Alcaligenes / Q000201:enzymology*; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D007527:Isoenzymes / Q000737:chemistry*; D009572:Nitrite Reductases / Q000737:chemistry*; D011487:Protein Conformation
D007527:Isoenzymes; D003300:Copper; D009572:Nitrite Reductases