Effect of alpha-hederin and sapindoside B on hepatic microsomal cytochrome P-450 in mice.
OBJECTIVE
To study the relation between the effect of alpha-hederin (Hed) and sapindoside B (Sap B) on cytochrome P-450 and their hepatoprotection.


METHODS
Mice were given sc Hed, Sap B, as well as a mixture of Hed + Sap B (1:1.5), and hepatic microsomal cytochrome P-450 was examined.


RESULTS
Hed 20 mg.kg-1, Sap B 20 mg.kg-1 and Hed + Sap B 20 mg.kg-1 sc, reduced hepatic P-450 content by 40%, 55% and 50%, respectively. The effect of the saponins on P-450 was reversible, as P-450 level returned to normal after 3 d. Phenobarbital (Phe) 50 mg.kg-1 ip increased P-450 2.5-fold, the Phe-induced P-450 was inhibited 50% by Hed + Sap B. No inhibitory effect was seen when liver microsomes were incubated with Hed + Sap B in vitro.


CONCLUSIONS
The hepatoprotective effects of Hed and Sap B were at least in part, due to its suppressive effect on liver cytochrome P-450.
Shi
J Z
JZ
Guizhou Institute of Traditional Chinese Medicine, China.
Liu
G T
GT
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010634:Phenobarbital / Q000037:antagonists & inhibitors; D012503:Saponins / Q000494:pharmacology*
D004365:Drugs, Chinese Herbal; D012503:Saponins; C010177:beta-hederin; C061619:sapindoside B; D009828:Oleanolic Acid; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital