title: Role of serum in regulating the Entamoeba histolytica cell cycle: a flowcytometric analysis.
abstract: To identify stage-specific molecules that might be important in pathogenicity, we focused on synchronizing Entamoeba histolytica HM1:1MSS axenic cultures by serum deprivation. By growing trophozoites in medium containing 2% serum for 16 h and then replacing with complete medium, we found that most of the trophozoites were in the G0/G1 phase of the cell cycle. Subsequently, the distribution of trophozoites in G0/G1, S and G2/M phases was studied for up to 24 h and it was observed that after 8 h, the trophozoites began to come out of the quiescent G0/G1 phase and the S and G2/M populations increased, indicating that certain serum factors regulate induction of cell division. It will be important to investigate these factors to find serum substitutes for cultivation media.
authors.affiliation: Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
authors.forename: H
authors.initials: H
authors.lastname: Vohra
authors.forename: R C
authors.initials: RC
authors.lastname: Mahajan
authors.forename: N K
authors.initials: NK
authors.lastname: Ganguly
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D016895:Culture Media, Serum-Free; D004748:Entamoeba histolytica / Q000254:growth & development*; D005434:Flow Cytometry; D007399:Interphase; D008018:Life Cycle Stages; D008938:Mitosis; D016196:S Phase
chemical_list: D016895:Culture Media, Serum-Free