On the electronic nature of low-barrier hydrogen bonds in enzymatic reactions.
The electronic nature of low-barrier hydrogen bonds (LBHBs) in enzymatic reactions is discussed based on combined low temperature neutron and x-ray diffraction experiments and on high level ab initio calculations by using the model substrate benzoylacetone. This molecule has a LBHB, as the intramolecular hydrogen bond is described by a double-well potential with a small barrier for hydrogen transfer. From an "atoms in molecules" analysis of the electron density, it is found that the hydrogen atom is stabilized by covalent bonds to both oxygens. Large atomic partial charges on the hydrogen-bonded atoms are found experimentally and theoretically. Therefore, the hydrogen bond gains stabilization from both covalency and from the normal electrostatic interactions found for long, weak hydrogen bonds. Based on comparisons with other systems having short-strong hydrogen bonds or LBHBs, it is proposed that all short-strong and LBHB systems possess similar electronic features of the hydrogen-bonded region, namely polar covalent bonds between the hydrogen atom and both heteroatoms in question.
Schiøtt
B
B
Department of Chemistry, University of California, Santa Barbara, CA 93106, USA.
Iversen
B B
BB
Madsen
G K
GK
Larsen
F K
FK
Bruice
T C
TC
D002074:Butanones / Q000737:chemistry*; D004798:Enzymes / Q000737:chemistry* / Q000378:metabolism*; D006860:Hydrogen Bonding; D008956:Models, Chemical; D008958:Models, Molecular; D008968:Molecular Conformation; D009502:Neutrons; D012542:Scattering, Radiation; D014961:X-Ray Diffraction
D002074:Butanones; D004798:Enzymes; C035851:benzoylacetone