Discordant effects of activator protein-1 transcription factor on gene regulation, invasion, and metastasis in spontaneous, radiation-induced, and fos-induced osteosarcomas.
Bone cells are a prime target for the biological function of the fos/jun (activating protein-1 (AP-1)) transcription factor complex. Deregulated expression of c-fos or v-fos in bone cells induces tumorigenicity and the formation of non-metastatic osteosarcomas. In contrast, fos oncogenes transform fibroblasts to an invasive phenotype accompanied by the expression of various invasion- and metastasis-associated genes. Here we compared the expression of AP-1-dependent genes and AP-1 activity in cell lines from fos-induced, radiation-induced, and spontaneous osteosarcomas. We showed that the presence of high AP-1 activity was not sufficient for the induction of invasion- and metastasis-associated AP-1-dependent genes in transformed bone cells. Further, we identified the collagenase I and stromelysin 1 gene promoters as suitable tools for the analysis of other factors regulating metastatic progression of osteosarcoma.
Rupp
B
B
Institute of Pathology, Technical University of Munich, München, Germany.
Lorenz
U
U
Schmidt
J
J
Werenskiold
A K
AK
D000818:Animals; D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology; D017931:DNA Primers; D015972:Gene Expression Regulation, Neoplastic; D016762:Genes, fos; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009381:Neoplasms, Radiation-Induced / Q000235:genetics* / Q000473:pathology; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D018808:Transcription Factor AP-1 / Q000378:metabolism*
D017931:DNA Primers; D018808:Transcription Factor AP-1