Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.
Techniques currently available to obtain anti-idiotypic reagents reactive with a single chain of a lymphocyte antigen receptor rely on immunization with intact soluble or cell-bound Ig or T-cell receptors. Ready recovery of single-chain-specific monoclonal antibodies (MAbs) depends on the presence of an immunodominant epitope on the desired chain and chance recovery of the responding clone. Here we present a method to maximize recovery of an Ig heavy-chain-specific anti-idiotypic Ig, using sequential immunization with MAbs expressing the H chain V region in combination with different H chain isotypes and with different light chains. The latter was produced by in vitro transfection of an H-chain-loss variant myeloma cell line with a transgene construct expressing the Ig H chain V region of interest. Sequential immunization may be a useful strategy to enhance selection of anti-Id reagents reactive with single chain-specific epitopes.
Foster
M H
MH
Department of Medicine, The University of Pennsylvania School of Medicine, Philadelphia 19104, USA.
Cooperstone
B G
BG
Chen
H
H
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000276:immunology*; D007797:Laminin / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D051381:Rats
D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D007797:Laminin