Effects of resuscitation fluids on T cell immune responses.
This study was designed to determine whether dextran, gelatin or hydroxyethyl starch-based colloidal resuscitation fluids (CRF) are inhibitory to T lymphocyte activation and mitogenesis in vitro. Isolated peripheral blood lymphocytes from normal donors were activated with mitogen (PHA) and cultured in up to 50% v:v CRF. Dual-label flow cytometry for CD69 and CD25 were used to assess early and full T cell activation responses, respectively, and thymidine incorporation was used to assess mitogenesis. T cell activation and mitogenic responses were not inhibited in the presence of CRF, implying that any systemic immunodepression associated with CRF infusion is not directly related to CRF-mediated impairment of T cell activation.
Sillett
H K
HK
Cancer Medicine Research Unit, Research School of Medicine, St James's University Hospital, Leeds.
Whicher
J T
JT
Trejdosiewicz
L K
LK
D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018929:Cell Culture Techniques; D003911:Dextrans / Q000494:pharmacology; D005780:Gelatin / Q000494:pharmacology; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology; D007108:Immune Tolerance / Q000187:drug effects*; D037181:Lectins, C-Type; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000276:immunology; D010952:Plasma Substitutes / Q000494:pharmacology*; D015375:Receptors, Interleukin-2 / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D003911:Dextrans; D005780:Gelatin; D006895:Hydroxyethyl Starch Derivatives; D037181:Lectins, C-Type; D010835:Phytohemagglutinins; D010952:Plasma Substitutes; D015375:Receptors, Interleukin-2; D000099192:CD69 Antigens