title: The two-exon gene of the human forkhead transcription factor FREAC-2 (FKHL6) is located at 6p25.3.
abstract: The gene for the human transcription factor forkhead related activator 2 (FREAC-2; HGMW-approved symbol FKHL6) has been characterized and found to consist of two exons separated by an intron of 3.6 kb. The first exon encodes the forkhead DNA-binding domain and one of the transcriptional activation domains, AD2. The second exon contains the coding sequence corresponding to the C-terminal activation domain AD1. The full-length FREAC-2 protein is predicted to be 444 amino acids, which adds 39 amino acids to the previously published partial cDNA sequence. A 2-kb CG island is centered around the 5' end of the FREAC-2 gene. Fluorescence in situ hybridization was used to localize the human FREAC-2 gene to chromosomal position 6p24-p25, and the localization was further refined by radiation hybrid mapping to 6p25.3.
authors.affiliation: Department of Molecular Biology, Göteborg University, Göteborg, Medicinaregatan 9C, S-413 90, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Blixt
authors.forename: M
authors.initials: M
authors.lastname: Mahlapuu
authors.forename: C
authors.initials: C
authors.lastname: Bjursell
authors.forename: C
authors.initials: C
authors.lastname: Darnfors
authors.forename: T
authors.initials: T
authors.lastname: Johannesson
authors.forename: S
authors.initials: S
authors.lastname: Enerbäck
authors.forename: P
authors.initials: P
authors.lastname: Carlsson
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002874:Chromosome Mapping; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D016384:Consensus Sequence; D018899:CpG Islands; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005091:Exons; D051858:Forkhead Transcription Factors; D006801:Humans; D006822:Hybrid Cells; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C486413:FOXF2 protein, human; D051858:Forkhead Transcription Factors; C114562:LUN protein, vertebrate; D015534:Trans-Activators