Inoculum preparation for in-vitro susceptibility testing of filamentous fungi.
The percent transmission (T) and the equivalence in cfu/mL of two suspensions of 125 isolates of clinically important filamentous fungi containing 1-5 x 10(5) and 1-5 x 10(4) conidia/mL, adjusted with a haemocytometer, were determined. A wide variation of T values was observed, especially with the first inoculum. A good agreement was obtained between the counted and the viable propagules. The haemocytometric method is considered a useful technique for standardizing inocula.
Guarro
J
J
Unitat de Microbiologia, Facultat de Medicina i Ciències de la Salut, Universitat Rovira i Virgili, Reus, Spain.
Pujol
I
I
Aguilar
C
C
Llop
C
C
Fernández-Ballart
J
J
D000164:Acremonium / Q000187:drug effects; D000935:Antifungal Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D005093:Exophiala / Q000187:drug effects; D005670:Fusarium / Q000187:drug effects; D008826:Microbial Sensitivity Tests / Q000379:methods* / Q000592:standards; D010143:Paecilomyces / Q000187:drug effects; D013053:Spectrophotometry / Q000379:methods
D000935:Antifungal Agents