title: Cathepsin D and dynamic magnetic resonance imaging gadolinium enhancement in malignant and benign breast lesions.
abstract: Our purpose was to determine whether the expression of cathepsin D, a proteolytic enzyme implicated in basement membrane degradation, is associated with dynamic magnetic resonance imaging (MRI) enhancement of breast lesions. Forty-five patients with 48 breast lesions underwent gadolinium-enhanced spoiled gradient recalled echo MRI followed by excisional biopsy and cathepsin D staining and semiquantitative measurement in the lesions. There was no significant difference in cathepsin D staining of 25 malignant and 23 benign breast lesions. A significant association was seen between high cathepsin D staining and positive axillary lymph nodes in invasive carcinomas. Nine of nine (100%) node-positive carcinomas had high cathepsin D, as compared to three of seven (43%) node-negative carcinomas (P = 0.02). No significant associations were observed between cathepsin D staining and MRI enhancement amplitude, rate, or washout. Cathepsin D has no effect upon MRI gadolinium enhancement of malignant and benign breast lesions but is associated with positive axillary lymph nodes in invasive carcinomas.
authors.affiliation: Division of Diagnostic Imaging, State University of New York at Buffalo, New York, 14263, USA. stomper@sc3102.med.buffalo.edu
authors.forename: P C
authors.initials: PC
authors.lastname: Stomper
authors.forename: J S
authors.initials: JS
authors.lastname: Winston
authors.forename: S
authors.initials: S
authors.lastname: Herman
authors.forename: D L
authors.initials: DL
authors.lastname: Klippenstein
authors.forename: L E
authors.initials: LE
authors.lastname: Blumenson
mesh_terms: D001706:Biopsy; D001940:Breast / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D001941:Breast Diseases / Q000201:enzymology* / Q000473:pathology*; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology*; D002402:Cathepsin D / Q000032:analysis*; D003287:Contrast Media; D005260:Female; D005682:Gadolinium; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008279:Magnetic Resonance Imaging; D009361:Neoplasm Invasiveness
chemical_list: D003287:Contrast Media; D005682:Gadolinium; D002402:Cathepsin D