Hierarchical order in a satellite DNA from the European brown frog Rana dalmatina.
We have cloned and sequenced a 341 bp EcoRV fragment of a repetitive DNA from the European brown frog Rana dalmatina. This fragment contained 11 copies in tandem of the 31 bp long repeat of a satellite DNA, that was named RdS2. RdS2 is species-specific, is not A+T rich and represents about 0.24% of the frog genome (3.5 x 10(5) copies of repeats per haploid genome). Southern blots of Rana dalmatina. DNA partially digested with EcoRV and RsaI indicated that RdS2 also contains high-order repeats with periodicities varying from 10 to 26 copies of the 31 bp unit repeat. Thus, RdS2 appears to have a hierarchical order like a number of satellite DNAs from other vertebrate and invertebrate genomes.
Cardone
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Dipartimento di Medicina Clinica e Sperimentale, Facoltà di Medicine e Chirurgia, Università di Napoli Federico II.
Feliciello
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Chinali
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D000818:Animals; D001001:Anura / Q000235:genetics*; D001483:Base Sequence; D004276:DNA, Satellite / Q000235:genetics*; D016678:Genome; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
D004276:DNA, Satellite