Abnormal IL-1beta cytokine expression in the cerebellum of the ataxic mutant mice staggerer and lurcher.
To assess the extent to which interleukin-1beta (IL-1beta) may contribute to the development and/or progression of neurodegenerative processes, we have examined the levels of IL-1beta in the brain of two types of neurological mutant mice, staggerer and Lurcher. Using a quantitative immunological method (enzyme-linked immunosorbent assay, ELISA), we measured IL-1beta in the cerebellum, hippocampus and cerebral cortex of mutant mice at baseline and after peripheral LPS treatment. Two types of IL-1beta expression abnormalities were found in the mutant cerebella: higher basal level in Lurcher and a response to peripheral administration of LPS in staggerer. The association of IL-1beta expression abnormalities with the only brain structure where a massive neurodegeneration occurs supports the role of proinflammatory cytokines in this process.
Vernet-der Garabedian
B
B
Laboratoire de Neurobiologie du Développement, Institut des Neurosciences (CNRS UMR 7624), Université Pierre et Marie Curie, 9 quai Saint Bernard, 75005, Paris, France. beatrice.vernet-dergarabedian@snv.jussieu.fr
Lemaigre-Dubreuil
Y
Y
Delhaye-Bouchaud
N
N
Mariani
J
J
D000818:Animals; D002524:Cerebellar Ataxia / Q000235:genetics / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D019446:Endotoxemia / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008818:Mice, Neurologic Mutants; D009410:Nerve Degeneration; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism*
D007375:Interleukin-1; D008070:Lipopolysaccharides; D009419:Nerve Tissue Proteins