title: Long chain coenzyme A esters activate the pore-forming subunit (Kir6. 2) of the ATP-regulated potassium channel.
abstract: The ATP-dependent potassium (KATP) channel in the pancreatic beta-cell is a complex of two proteins, the pore-forming Kir6.2 and the sulfonylurea receptor type 1 (SUR1). Both subunits are required for functional KATP channels because expression of Kir6.2 alone does not result in measurable currents. However, truncation of the last 26 or 36 amino acids of the C terminus of Kir6.2 enables functional expression of the pore-forming protein in the absence of SUR1. Thus, by using the truncated form of Kir6.2, expressed in the absence and presence of SUR1, it has been shown that the site at which ATP mediates channel inhibition is likely to be situated on Kir6.2. We have now examined the effects of long chain acyl-CoA (LC-CoA) esters on the C-terminally truncated mouse Kir6.2Delta365-390 (Kir6. 2DeltaC26) in inside-out patches isolated from Xenopus laevis oocytes. LC-CoA esters, saturated (C14:0, C16:0) and unsaturated (C18:1), increased Kir6.2DeltaC26 currents, whereas short and medium chain CoA esters (C3:0, C8:0, C12:0) were unable to affect channel activity. The LC-CoA esters were also able to counteract the blocking effect of ATP on Kir6.2DeltaC26. The stimulatory effect of the esters could be explained by the induction of a prolonged open state of Kir6.2DeltaC26. In the presence of the esters, channel open time was increased approximately 3-fold, which is identical to what was obtained in the native mouse KATP channel. Coexpression of SUR1 together with Kir6.2DeltaC26 did not further increase the ability of LC-CoA esters to stimulate channel activity. We conclude that Kir6.2 is the primary target for LC-CoA esters to activate the KATP channel and that the esters are likely to induce a conformational change by a direct interference with the pore-forming subunit, leading to openings of long duration.
authors.affiliation: Rolf Luft Center for Diabetes Research, Department of Molecular Medicine, Karolinska Institute, S-171 76 Stockholm, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Bränström
authors.forename: I B
authors.initials: IB
authors.lastname: Leibiger
authors.forename: B
authors.initials: B
authors.lastname: Leibiger
authors.forename: B E
authors.initials: BE
authors.lastname: Corkey
authors.forename: P O
authors.initials: PO
authors.lastname: Berggren
authors.forename: O
authors.initials: O
authors.lastname: Larsson
mesh_terms: D018528:ATP-Binding Cassette Transporters; D000214:Acyl Coenzyme A / Q000737:chemistry / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D004553:Electric Conductivity; D004594:Electrophysiology / Q000379:methods; D015640:Ion Channel Gating; D054086:KATP Channels; D007700:Kinetics; D051379:Mice; D009865:Oocytes; D010446:Peptide Fragments / Q000187:drug effects; D015221:Potassium Channels / Q000172:deficiency / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D024661:Potassium Channels, Inwardly Rectifying; D011955:Receptors, Drug / Q000172:deficiency; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D064233:Sulfonylurea Receptors; D014982:Xenopus laevis
chemical_list: D018528:ATP-Binding Cassette Transporters; C577508:Abcc8 protein, mouse; D000214:Acyl Coenzyme A; D054086:KATP Channels; D010446:Peptide Fragments; D015221:Potassium Channels; D024661:Potassium Channels, Inwardly Rectifying; D011955:Receptors, Drug; D011994:Recombinant Proteins; D064233:Sulfonylurea Receptors; C092520:uK-ATP-1 potassium channel; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate