Characterization of antibody against the N-terminus of RDS/peripherin.
Mutations in the gene encoding RDS/peripherin cause a variety of retinal disorders. Attempts to model such disorders in vitro and in vivo have been hampered by the paucity of available immunological reagents. Moreover, available antibodies have been generated from undefined or C-terminal epitopes and therefore may not suitable for detecting all known RDS/peripherin mutants. We consequently generated affinity-purified rabbit antibody against a 14 amino acid peptide corresponding to the highly conserved N-terminus of human RDS/ peripherin. This new antibody, N-RDS, recognizes RDS/peripherin in the retina of man, macaque, and rat. N-RDS may prove useful in studying RDS/peripherin mutants, particularly those with abnormal C-terminal domains.
Kim
R Y
RY
Kimura Laboratory of Clinical Investigation, Beckman Vision Center, Department of Ophthalmology, University of California, San Francisco, USA.
Bedolli
M A
MA
Goodarzi
J
J
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting; D007150:Immunohistochemistry / Q000379:methods; D007381:Intermediate Filament Proteins / Q000276:immunology* / Q000378:metabolism; D008251:Macaca; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins / Q000276:immunology* / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology*; D064531:Peripherins; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000378:metabolism
D000906:Antibodies; D007106:Immune Sera; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; C578994:PRPH protein, human; C578997:PRPH2 protein, human; D010446:Peptide Fragments; D064531:Peripherins; C579003:Prph2 protein, rat