Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X-ray crystallography.
The oxalate oxidase enzyme expressed in barley roots is a thermostable, protease-resistant enzyme that generates H2O2. It has great medical importance because of its use to assay plasma and urinary oxalate, and it has also been used to generate transgenic, pathogen-resistant crops. This protein has now been purified and three types of crystals grown. X-ray analysis shows that the symmetry present in these crystals is consistent with a hexameric arrangement of subunits, probably a trimer of dimers. This structure may be similar to that found in the related seed storage proteins.
Woo
E J
EJ
School of Plant Sciences, The University of Reading, Whiteknights, UK.
Dunwell
J M
JM
Goodenough
P W
PW
Pickersgill
R W
RW
D018360:Crystallography, X-Ray; D001467:Hordeum / Q000201:enzymology*; D010088:Oxidoreductases / Q000737:chemistry*
D010088:Oxidoreductases; C020598:oxalate oxidase