The Krüppel-type zinc finger family gene, HKR1, is induced in lung cancer by exposure to platinum drugs.
To investigate the molecular mechanism associated with the signaling pathway of platinum drug administration, we focused on the C2H2-type zinc finger (ZNF) transcription factor gene family. Here we show cloning of a Krüppel-type ZNF gene, HKR1, which contains Krüppel-associated box (KRAB) domain and ZNF motifs. We found that mRNA expression of the HKR1 gene was induced in lung-cancer cell lines by exposure to cisplatin using Northern blot analysis. Moreover, we also found that HKR1 mRNA expression levels in lung cancers were higher than those in normal lung tissues, and that high expression levels in lung cancers were associated with antemortem platinum drug administration. These results suggest that HKR1 may be associated with the regulation of a signaling pathway involved in the progression of lung cancer or the acquisition of resistance to platinum drugs.
Oguri
T
T
Second Department of Internal Medicine, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan.
Katoh
O
O
Takahashi
T
T
Isobe
T
T
Kuramoto
K
K
Hirata
S
S
Yamakido
M
M
Watanabe
H
H
D000230:Adenocarcinoma / Q000235:genetics*; D000595:Amino Acid Sequence; D001344:Autopsy; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D016190:Carboplatin / Q000494:pharmacology; D002945:Cisplatin / Q000494:pharmacology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D008175:Lung Neoplasms / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins; D017671:Platinum Compounds / Q000494:pharmacology*; D011506:Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D001426:Bacterial Proteins; D018076:DNA, Complementary; D051741:Kruppel-Like Transcription Factors; D009687:Nuclear Proteins; D017671:Platinum Compounds; D011506:Proteins; D014157:Transcription Factors; C086036:ZNF875 protein, human; D016190:Carboplatin; D002945:Cisplatin