Suppression of DNA synthesis of cultured mesothelial cells by supplemented albumin.
Mesothelial cells, harvested from rat omentum, were cultured with 10% FCS/MEM till the third passage, and then seeded at a density of 2-3 x 10(4)/cm2 in a 96 well micro-titer plate. Both albumin and glucose were added to each culture medium at final concentrations of 0-2 g/dl and 0-90 mM, respectively. On days 2 and 3, 5-bromo-2'-deoxy-uridine (BrdU) was added and incubated for 24-h to be incorporated into cellular DNA and detected by the ELISA principle. The lowest DNA synthesis was seen when both 2.0 g/dl of albumin and 90 mM of glucose were supplemented to the medium while the highest was obtained without albumin supplement. In the range of 0-90 mM of glucose and 0-2 g/dl of albumin, generally speaking, DNA synthesis of cultured mesothelial cells could be suppressed by either glucose or albumin, and it was greatly enhanced when both concentrations increased.
Horiuchi
T
T
Division of Applied Life Science, Graduate School of University of East Asia, Yamaguchi, Japan. horiuchi@po.pios.toua-u.ac.jp
Hayashi
K
K
Kumano
K
K
D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D002452:Cell Count; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D009852:Omentum / Q000033:anatomy & histology; D051381:Rats; D017208:Rats, Wistar
D000418:Albumins; D003470:Culture Media; D004247:DNA; D005947:Glucose