A screen for lethal mutations in the chromosomal region 59AB suggests that bellwether encodes the alpha subunit of the mitochondrial ATP synthase in Drosophila melanogaster.
We report the isolation and complementation mapping of lethal mutations within the 59AB region on the second chromosome of Drosophila melanogaster. The newly induced lethal mutations in this region define four different complementation groups. Using existing and newly induced deficiencies, these loci can be assigned to three different chromosomal intervals. Moreover, complementation analysis with chromosomes carrying various P element insertions, in combination with a molecular characterization of the corresponding insertion sites, suggests that the previously described male sterile mutation bellwether is an allele of an essential gene that encodes the alpha subunit of the mitochondrial ATP synthase.
Jacobs
H
H
Department of Genetics, University of Bayreuth, Germany.
Stratmann
R
R
Lehner
C
C
D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D019926:Cyclin B / Q000235:genetics; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial / Q000235:genetics; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005804:Genes, Lethal; D005816:Genetic Complementation Test; D008297:Male; D008969:Molecular Sequence Data; D009154:Mutation; D006180:Proton-Translocating ATPases / Q000235:genetics*
C503698:CycB protein, Drosophila; D019926:Cyclin B; D004251:DNA Transposable Elements; D004272:DNA, Mitochondrial; D029721:Drosophila Proteins; D006180:Proton-Translocating ATPases