Degradation of transferrin and albumin by radical reactions in human plasma evaluated by immunoblot.
Immunoblot analysis showed that transferrin and human serum albumin were degraded in a similar rate on the basis of the protein weight by radical reactions using a solution containing each protein initiated by Cu2+ and hydrogen peroxide. When plasma was treated with Cu2+ and hydrogen peroxide, the rate profile of the degradation of transferrin was similar to that of albumin based on immunoblot analysis in spite that the content of these proteins in plasma was considerably different. These results suggest that highly reactive hydroxyl radicals attack these proteins indiscriminately. Present observations demonstrate that immunoblot analysis is an effective method to analyze radical reactions of each protein in the presence of many other proteins like plasma.
Yamada
Y
Y
Department of Food Science and Nutrition, Nara Women's University, Japan.
Okamoto
E
E
Tanaka
K
K
Kojo
S
S
D015153:Blotting, Western; D005609:Free Radicals; D006801:Humans; D006868:Hydrolysis; D012709:Serum Albumin / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism*
D005609:Free Radicals; D012709:Serum Albumin; D014168:Transferrin