Discrimination among Pediocin AcH/PA-1 producer strains by comparison of pedB and pedD amplified genes and by multiplex PCR assay.
Pediococcus acidilactici, Pediococcus parvulus and Lactobacillus plantarum pediocin AcH/PA-1 produces strains were studied with the aim to investigate their common genetic pediocin determinant using pedA, pedB, pedC and pedD gene-targeted PCR assay. Single Strand Conformation Polymorphism and restriction analysis of pedA and pedC amplified fragments from the three different species did not show any differences in sequence while these analysis carried out on pedB and pedD amplified fragments highlighted differences related to the three species analyzed harboring these plasmid encoded genes. Furthermore different multiplex PCR assay using IdhD, pedA and pedD as target genes were developed to clearly identify the pediocin AcH/PA-1 producer strains and to obtain the simultaneous identification of the P. acidilactici strains.
Mora
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D
Department of Food Science and Microbiology, Industrial Microbiology Section, University of Milano, Italy. diegom@net4u.it
Parini
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C
Fortina
M G
MG
Manachini
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PL
D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D015139:Blotting, Southern; D005784:Gene Amplification; D005798:Genes, Bacterial; D007778:Lactobacillus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D009876:Operon / Q000235:genetics; D000070017:Pediocins; D010376:Pediococcus / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA
D001430:Bacteriocins; D000070017:Pediocins; C074288:pediocin PA-1