A semiquantitative analysis of the effects of cisplatin on the rat stria vascularis.
Cisplatin (CDDP) is a very effective chemotherapeutic agent but is highly ototoxic. Most studies have focused on the effects of CDDP on the outer hair cells. The purpose of this study was to examine changes in the stria vascularis in cisplatin treated male Wistar rats and to provide semiquantitative analysis of the results. We removed a section of the stria vascularis from the basal turn of five control and five CDDP (16 mg/kg) treated rats. Using transmission electron microscopy (TEM) we analyzed: (1) changes to the strial tissue as a whole; and (2) intracellular changes in the marginal cells. We also subjected the samples to semiquantitative analysis using the MCID, focusing on three aspects of strial profile abnormalities; the number of abnormal marginal cells in CDDP treated tissue, intracellular strial edema and densitometry. Controls appeared normal, but many pathologic changes were apparent in the experimental group. Results from the semiquantitative analysis indicate cisplatin has a deleterious effect on the stria vascularis including strial edema; bulging, rupture and/or compression of the marginal cells and depletion of the cytoplasmic organelles.
Meech
R P
RP
Department of Surgery, SIU School of Medicine, Springfield, IL 62794-1618, USA.
Campbell
K C
KC
Hughes
L P
LP
Rybak
L P
LP
D000818:Animals; D000970:Antineoplastic Agents / Q000506:poisoning*; D002945:Cisplatin / Q000506:poisoning*; D015834:Cochlear Diseases / Q000139:chemically induced / Q000473:pathology; D003593:Cytoplasm / Q000187:drug effects / Q000648:ultrastructure; D004487:Edema / Q000139:chemically induced / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D015388:Organelles / Q000187:drug effects / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D013316:Stria Vascularis / Q000187:drug effects* / Q000473:pathology
D000970:Antineoplastic Agents; D002945:Cisplatin