title: Isolation of single chain antibody fragments with specificity for cell surface antigens by phage display utilizing internal image anti-idiotypic antibodies.
abstract: Recombinant single chain antibody fragments (scFv) with specificity for membrane-bound antigens can be isolated by phage display techniques. The strategy involves selection of recombinant phage antibodies by binding to cells expressing the respective antigen. This results frequently in high nonspecific adherence of phages to cellular membranes. To resolve the problem we have made use of an internal image anti-idiotypic antibody mimicking the membrane-bound CD30 antigen and successfully isolated scFv fragments with specificity for CD30. The cDNA coding for the immunoglobulin heavy and light chain variable regions of the anti-CD30 monoclonal antibody (mAb) HRS3 was expressed by phage display techniques. Recombinant HRS3-scFv phages were efficiently enriched by one cycle of panning on the internal image anti-idiotypic mAb 9G10. The isolated HRS3-scFv clone retained the binding specificity of the parental mAb HRS3 to the internal image anti-idiotypic mAb 9G10 as well as to an anti-idiotypic mAb without the internal image. Furthermore HRS3-scFv reacted with recombinant and cell-bound CD30 antigen, respectively. Binding of scFv fragments to anti-idiotypic mAbs will provide a versatile strategy for the efficient isolation of recombinant antibody fragments.
authors.affiliation: Klinik I für Innere Medizin, Labor Tumorgenetik, Universität zu Köln, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hombach
authors.forename: C
authors.initials: C
authors.lastname: Pohl
authors.forename: C
authors.initials: C
authors.lastname: Heuser
authors.forename: R
authors.initials: R
authors.lastname: Sircar
authors.forename: V
authors.initials: V
authors.lastname: Diehl
authors.forename: H
authors.initials: H
authors.lastname: Abken
mesh_terms: D000888:Antibodies, Anti-Idiotypic / Q000235:genetics / Q000276:immunology*; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology; D000918:Antibody Specificity; D019084:Fluorescent Antibody Technique, Indirect; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000235:genetics / Q000302:isolation & purification*; D007135:Immunoglobulin Variable Region / Q000235:genetics / Q000302:isolation & purification; D017730:Ki-1 Antigen / Q000276:immunology; D019151:Peptide Library; D011994:Recombinant Proteins / Q000302:isolation & purification; D014407:Tumor Cells, Cultured
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000911:Antibodies, Monoclonal; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D017730:Ki-1 Antigen; D019151:Peptide Library; D011994:Recombinant Proteins