Cardiac myocytes express mRNA for ten RGS proteins: changes in RGS mRNA expression in ventricular myocytes and cultured atria.
Regulators of G-protein signalling (RGS) are recently identified proteins that shorten the lifetime of the activated G protein. We now show that rat cardiac myocytes express mRNA for at least 10 RGS. The mRNA for RGS-r is barely detectable in rat ventricles, but increases more than 20-fold during the 60- to 90-min process of isolating ventricular myocytes, and after 90 min of culture of atrial pieces in medium with Ca2+. Both in myocytes and in atria, the rise in RGS-r is transient. The mRNA for cardiac RGS5, but not RGS-r, is developmentally regulated. These studies suggest that rapid regulation of RGS levels may be a new mechanism that governs how signals are transmitted across the cardiac cell membrane.
Kardestuncer
T
T
Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
Wu
H
H
Lim
A L
AL
Neer
E J
EJ
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000502:physiology; D002478:Cells, Cultured; D005136:Eye Proteins / Q000096:biosynthesis / Q000235:genetics*; D019204:GTP-Binding Proteins / Q000096:biosynthesis / Q000235:genetics*; D020690:GTPase-Activating Proteins; D005786:Gene Expression Regulation; D006321:Heart / Q000254:growth & development; D006325:Heart Atria; D006352:Heart Ventricles; D007700:Kinetics; D009206:Myocardium / Q000378:metabolism*; D009928:Organ Specificity; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics*; D020710:RGS Proteins; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014158:Transcription, Genetic
D005136:Eye Proteins; D020690:GTPase-Activating Proteins; D011506:Proteins; D020710:RGS Proteins; C491400:RGS14 protein, human; C107413:RGS16 protein; C491349:RGS5 protein, human; C492039:RGS7 protein, human; D012333:RNA, Messenger; C489853:Rgs1 protein, rat; C491401:Rgs12 protein, rat; C491399:Rgs14 protein, mouse; C106374:Rgs14 protein, rat; C491348:Rgs5 protein, rat; C492038:Rgs7 protein, mouse; C115771:Rgs7 protein, rat; C098799:RGS4 protein; D019204:GTP-Binding Proteins