Establishment of a serum-free culture cell line, CPK-NS, which is useful for assays of classical swine fever virus.
A stable porcine kidney cell line, CPK-NS, was established and maintained in serum-free culture. A cytopathic effect (CPE) was observed clearly in CPK-NS cells infected with some classical swine fever virus (CSFV) strains which did not show the exaltation of Newcastle disease virus (END) phenomenon. Chromosome condensation and DNA fragmentation, a marker for apoptosis, were detected in cells infected with END phenomenon-negative CSFV strains. By using the CPE induced by infection with an END phenomenon-negative CSFV strain in CPK-NS cells, assays of CSFV were established. The virus titer determined in CPK-NS cells shows a high correlation with the usual peroxidase-linked assay, dome disappearance method and END method. Furthermore, the antibody titer by neutralizing test with CPK-NS cells also correlated with that measured by the usual neutralizing peroxidase-linked assay and dome disappearance method. These stable CPK-NS cells have the great advantage that a clear CPE was caused by infection with END phenomenon-negative CSFV strains and bovine serum is not necessary for cell culture and virus assays.
Sakoda
Y
Y
Department of Exotic Diseases, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Hikawa
M
M
Tamura
T
T
Fukusho
A
A
D000818:Animals; D002417:Cattle; D018929:Cell Culture Techniques; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000276:immunology / Q000502:physiology*; D016895:Culture Media, Serum-Free; D003588:Cytopathogenic Effect, Viral; D009500:Neutralization Tests; D013552:Swine
D016895:Culture Media, Serum-Free