Identification of a Chlamydia trachomatis serovar E urogenital isolate which lacks the cryptic plasmid.
Our laboratory recently recovered Chlamydia trachomatis in tissue culture from a urogenital specimen which tested negative by commercial plasmid-based PCR. Immunotyping and omp1 sequencing identified the isolate as a serovar E isolate. Further investigation by PCR and Southern hybridization indicated that this isolate lacks the chlamydial cryptic plasmid.
Stothard
D R
DR
Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. dstothar@iupui.edu
Williams
J A
JA
Van Der Pol
B
B
Jones
R B
RB
D000328:Adult; D001425:Bacterial Outer Membrane Proteins; D015373:Bacterial Typing Techniques; D002690:Chlamydia Infections / Q000175:diagnosis*; D002692:Chlamydia trachomatis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005188:False Negative Reactions; D006801:Humans; D008297:Male; D052801:Male Urogenital Diseases / Q000175:diagnosis*; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011933:Reagent Kits, Diagnostic
D001425:Bacterial Outer Membrane Proteins; D011933:Reagent Kits, Diagnostic