GABAA receptor subunit mRNAs in rat superior cervical ganglia.
gamma-Aminobutyric acid (GABA), one of the most important neurotransmitters in the brain, is also found in the periphery. GABAA receptors are chloride channels opened by GABA whose presence in the rat superior cervical ganglion has been indicated by functional and binding measurements. We describe the first molecular data on the possible subunit composition of these receptors, detecting mRNAs for 12 subunits (alpha1-5, beta1-3, gamma1-3, delta) by reverse-transcriptase polymerase chain reaction. Preliminary quantitation gave highest levels (in descending order) for the gamma2 (both short and long forms), beta3, gamma3, and alpha1 subunits.
Liu
Z F
ZF
Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, Md. 21201-1559, USA.
Burt
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D000818:Animals; D004587:Electrophoresis, Agar Gel; D005260:Female; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger; D011963:Receptors, GABA-A