Use of cell wall-less bacteria (L-forms) for efficient expression and secretion of heterologous gene products.
In spite of many efforts and achievements to optimize the prokaryotic expression systems, there are still general and specific problems in obtaining sufficient yields of the functionally active gene products. The main problems concern the formation of inclusion bodies, incorrect folding, toxicity for the producer cells and degradation by proteases. One way to overcome these problems is with expression systems alternative to those of Escherichia coli. For the first time, cell wall-less L-form bacteria were used to establish such an alternative expression system and test its practicability. The results showed that various recombinant proteins can be synthesized in considerable amounts as soluble, functionally active products with these cell wall-less strains.
Gumpert
J
J
Institute of Molecular Biotechnology, Jena, Germany. gumpert@imb-jena.de
Hoischen
C
C
D000818:Animals; D001419:Bacteria / Q000235:genetics*; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D011513:Proteus mirabilis / Q000235:genetics; D011523:Protoplasts / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism
D011994:Recombinant Proteins