title: Long-distance PCR-based strategy for preparing knock-in vectors directly from ES cell genomic DNA.
authors.affiliation: University of British Columbia, Vancouver, Canada.
authors.forename: L H
authors.initials: LH
authors.lastname: Zhang
authors.forename: H E
authors.initials: HE
authors.lastname: Henderson
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D004247:DNA / Q000235:genetics*; D017931:DNA Primers; D018390:Gene Targeting; D005822:Genetic Vectors / Q000235:genetics*; D008071:Lipoprotein Lipase / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D016297:Mutagenesis, Site-Directed; D016133:Polymerase Chain Reaction / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D011993:Recombinant Fusion Proteins; D004247:DNA; D008071:Lipoprotein Lipase