[Cysteine-dependent protease in Trypanosoma cruzi useful for the diagnosis of Chagas disease].
An immunoenzymatic system on solid phase for the detection of IgG antibodies in serum from chronic Chagasic patients was standardized. A protease from Trypanosoma cruzi (GP57/51KDa) was used as an antigen. The sensitivity, specificity and predictive values of the procedure were calculated taking into account the results obtained from conventional serology (CS), indirect immunofluorescence (IIF), indirect hemagglutination (IHA) and complement-mediated lysis test (CML) for the detection of anti-Trypanosoma cruzi antibodies in serum. Except one, the positive samples obtained by those techniques were also positive by our method. These results show that GP 57/51 is useful in the serodiagnosis of Chagas disease.
Alberti Amador
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E
Instituto de Medicina Tropical Pedro Kourí.
Fachado Carvajales
A
A
Montalvo
A M
AM
Izquierdo Pérez
L A
LA
Fonte Galindo
L
L
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000276:immunology; D014355:Chagas Disease / Q000175:diagnosis* / Q000276:immunology; D004796:Clinical Enzyme Tests; D003546:Cysteine Endopeptidases / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D014349:Trypanosoma cruzi / Q000201:enzymology* / Q000276:immunology
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D006023:Glycoproteins; D007074:Immunoglobulin G; D003546:Cysteine Endopeptidases; C073182:GP57-51 antigen, Trypanosoma cruzi
Proteasa dependiente de cisteína en Trypanosoma cruzi útil en el diagnóstico de la enfermedad de Chagas.