Analysis of clonal diversity in mouse immunoglobulin heavy chain genes selected for size of the antigen combining site.
Size-diversity of Ig and T cell receptor antigen binding (CDR3) regions can be visualized by "CDR3 fingerprinting", and provides an estimate of B- or T-cell repertoire complexity. The method does not identify clonal diversity, however, which can only be determined by random sequencing of the CDR3s. In this study we demonstrate that a combination of fingerprinting and single strand conformation polymorphism (SSCP) analysis can be used for a rapid estimation of clonal diversity within mouse Ig antigen binding regions selected for size. This application may be useful in the analysis of clonal expansion within B- and T-cell repertoires.
Raaphorst
F M
FM
Department of Microbiology, The University of Texas Health Science Center at San Antonio, 78284, USA.
Gokmen
E
E
Teale
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D000916:Antibody Diversity; D001483:Base Sequence; D001666:Binding Sites, Antibody / Q000235:genetics; D022801:Complementarity Determining Regions; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007137:Immunoglobulin alpha-Chains / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011948:Receptors, Antigen, T-Cell / Q000235:genetics
D022801:Complementarity Determining Regions; D017931:DNA Primers; D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains; D011948:Receptors, Antigen, T-Cell