Moesin becomes linked to the plasma membrane in attached neutrophil granulocytes.
Following 35 min of adhesion to a plastic surface, an 80-kDa F-actin-binding protein was shown to be enriched in the plasma membrane fractions of porcine neutrophils by protein blotting with labeled F-actin. This protein was almost undetectable in membrane fractions of free floating neutrophils, while it was present in total cell samples. The 80-kDa protein appeared to be a major high molecular mass component of the isolated actin-cytoskeleton of both control and attached cells. The studied F-actin-binding protein was recognized by anti-moesin antibodies. Our results suggest that moesin is translocated to the plasma membrane upon adhesion of neutrophils to the extracellular surface.
Keresztes
M
M
Department of Biochemistry, Albert Szent-Györgyi Medical University, 6701 Szeged, Hungary. margo@biochem.szote.u-szeged.hu
Lajtos
Z
Z
Fischer
J
J
Dux
L
L
D000199:Actins / Q000378:metabolism; D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008840:Microfilament Proteins / Q000378:metabolism*; D008970:Molecular Weight; D009504:Neutrophils / Q000378:metabolism*; D010969:Plastics; D013499:Surface Properties; D013552:Swine
D000199:Actins; D008840:Microfilament Proteins; D010969:Plastics; C071058:moesin