title: Superoxide electrode based on covalently immobilized cytochrome c: modelling studies.
abstract: We have recently described an optimised electrode for the detection of enzymatic and cellular superoxide (O2*-) production based on cytochrome c immobilized covalently at a surface-modified gold electrode and applied this system to the study of free radical production by activated human glioblastoma cells. In this paper we report the development of a mathematical model for the O2*- electrode responding to enzymically produced O2*- which should enable the determination of absolute concentrations of O2*- in biological systems when this electrode is employed for direct, real-time monitoring of free radical release and interactions.
authors.affiliation: Institute of Physical Chemistry, University of Tartu, Estonia.
authors.forename: K
authors.initials: K
authors.lastname: Tammeveski
authors.forename: T T
authors.initials: TT
authors.lastname: Tenno
authors.forename: A A
authors.initials: AA
authors.lastname: Mashirin
authors.forename: E W
authors.initials: EW
authors.lastname: Hillhouse
authors.forename: P
authors.initials: P
authors.lastname: Manning
authors.forename: C J
authors.initials: CJ
authors.lastname: McNeil
mesh_terms: D003574:Cytochrome c Group; D004563:Electrochemistry; D004566:Electrodes; D005909:Glioblastoma / Q000378:metabolism; D006801:Humans; D008433:Mathematics; D008956:Models, Chemical; D013481:Superoxides / Q000032:analysis* / Q000378:metabolism; D014407:Tumor Cells, Cultured; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D003574:Cytochrome c Group; D013481:Superoxides; D019820:Xanthine; D014969:Xanthine Oxidase