title: Advancements toward magneto immunoassays.
abstract: Recently, a magneto binding assay was conducted. The operational principle is based on a 'sandwich' mode of detection, where the target analyte (Concanavalin A) becomes bound nonselectively by protein adsorption between the solid support (silica carrier particles) and the ferromagnetic label (magneto markers). A magnetic transducer was employed for the detection. The binding assay gave a linear response in the dynamic range of 0-1.9 microM and proved to be sensitive by having a limit of detection at 250 nM. Furthermore, the relative standard deviation observed was 6.2% (n = 3). The demonstration of the 'sandwich' approach is a step toward achieving magneto immunoassays (MIA).
authors.affiliation: European Institute of Science, Lund, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Kriz
authors.forename: J
authors.initials: J
authors.lastname: Gehrke
authors.forename: D
authors.initials: D
authors.lastname: Kriz
mesh_terms: D000818:Animals; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008280:Magnetics; D012680:Sensitivity and Specificity