title: Clustered p53 immunostaining: a novel pattern associated with prostate cancer progression.
abstract: Abnormal p53 protein accumulation is typically defined as present when greater than 5 or 10% of cancer cells stain positively. We present a novel approach whereby immunopositivity is defined when 15 or more cells within a 300 x 400-micrometer(2) field exhibit p53 protein accumulation; a feature that we have called "clustered" staining. We assessed p53 immunostaining of moderately differentiated, clinically localized prostate cancers derived from two patient groups: those without cancer recurrence 5 years after radical prostatectomy, and those in whom cancer had recurred following radical prostatectomy. Clustered p53 immunopositivity was present in 10 (63%) of 16 patients in the recurrent group and in only 7 (21%) of 33 in the nonrecurrent group. Clustered p53 staining was clearly associated with cancer recurrence (P < 0.01). This refinement of a commonly used assay may help define the biological aggressiveness of a cancer.
authors.affiliation: Scott Department of Urology and Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: G
authors.initials: G
authors.lastname: Yang
authors.forename: A M
authors.initials: AM
authors.lastname: Stapleton
authors.forename: T M
authors.initials: TM
authors.lastname: Wheeler
authors.forename: L D
authors.initials: LD
authors.lastname: Truong
authors.forename: T L
authors.initials: TL
authors.lastname: Timme
authors.forename: P T
authors.initials: PT
authors.lastname: Scardino
authors.forename: T C
authors.initials: TC
authors.lastname: Thompson
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009364:Neoplasm Recurrence, Local; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000032:analysis* / Q000276:immunology
chemical_list: D016159:Tumor Suppressor Protein p53