Semiquantitative bioluminescent assay of glutathione.
A novel technique has been developed for semiquantitative detection of glutathione (GSH) in small volumes of liquid samples. GSH is detected via enzymatic linkage to the NADP/NADPH + H+ redox system through glutathione reductase. Accumulated NADPH is measured via the bioluminescent FMN oxidoreductase bacterial luciferase reaction. A linear correlation is obtained between bioluminescence intensity of the luciferase reaction and the GSH content of the liquid sample. Possible applications of this procedure are discussed.
Romero
F J
FJ
Institute for Physiology and Pathophysiology, University of Mainz, Germany.
Mueller-Klieser
W
W
D005978:Glutathione / Q000032:analysis*; D005980:Glutathione Reductase; D007202:Indicators and Reagents; D008156:Luciferases; D008163:Luminescent Measurements; D009249:NADP; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D007202:Indicators and Reagents; D009249:NADP; D008156:Luciferases; D005980:Glutathione Reductase; D005978:Glutathione