title: Adrenergic drug effects on cyclic AMP in cultured human trabecular meshwork cells.
abstract: Cyclic AMP production in the presence or absence of various adrenergic agonists and antagonists was determined in cultured human trabecular cells using a gammaflow automated radioimmunoassay that allowed multiple simultaneous experiments and reproducible results. Adrenergic agonists and antagonists showed activation and inhibition constants consistent with the presence of beta2-receptors: Ka of isoproterenol < epinephrine < norepinephrine < phenylephrine; Ki of timolol < betaxolol < celiprolol < atenolol. Selective ICI antagonists showed beta2-specificity.
authors.affiliation: Annete and Rosin Eye Department, Bnai-Zion Medical Center and Technicon Faculty of Medicine, Haifa, Israel.
authors.forename: Z
authors.initials: Z
authors.lastname: Friedman
authors.forename: E
authors.initials: E
authors.lastname: Bloom
authors.forename: J R
authors.initials: JR
authors.lastname: Polansky
mesh_terms: D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000328:Adult; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010784:Photomicrography; D011863:Radioimmunoassay; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
chemical_list: D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000242:Cyclic AMP