Augmentation in the expression of a ribosomal protein associated with transformation.
Using differential hybridization technique, cDNA that was a partial sequence of rat ribosomal protein L41 was isolated from fos- and src-transfected rat fibroblast 3Y1. The cDNA was named #31. Subsequently, the human counterpart(named hm3168) was cloned from a human melanoma cell line(M14) and the amino acid sequence deduced from the cDNA proved to be identical to that of HG-12. The gene expression of hm3168 was inducible by serum-stimulation. It reveals the possible up-regulation of the transcription via fos-mediated signal transduction. Northern-blot and Western-blot analyses showed a large expression of the #31 gene in the rat transformed cell lines.
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Department of Surgery, Kyushu University, Beppu, Japan.
Taniguchi
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D000818:Animals; D001769:Blood; D002460:Cell Line; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D015972:Gene Expression Regulation, Neoplastic; D016762:Genes, fos; D016391:Genes, src; D006801:Humans; D009693:Nucleic Acid Hybridization; D011506:Proteins / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D012269:Ribosomal Proteins / Q000235:genetics*; D015398:Signal Transduction; D014162:Transfection; D014407:Tumor Cells, Cultured
D018076:DNA, Complementary; D011506:Proteins; D012333:RNA, Messenger; D012269:Ribosomal Proteins; C076844:RPL41 protein, human