Nasal drug delivery--evaluation of an in vitro model using porcine nasal mucosa.
An in vitro model for permeation studies using porcine nasal mucosa was developed and evaluated. The viability and integrity of the mucosa were determined by electrophysiological measurements, permeation studies involving 14C-mannitol and D-(2-3H) glucose, histological studies and a biochemical assay. Enzymatic activity in the mucosa was determined by serosal addition of ouabain. Three different types of porcine nasal mucosa (cavity mucosa, natural septum mucosa and dermatomed septum mucosa) were examined. The results showed that cavity mucosa was the most suitable; this type remained viable for up to 8 h after removal. Lower limits for electrophysiological data were defined in order to establish criteria for tissue viability. This in vitro method using porcine nasal mucosa appears potentially valuable as a tool for further permeation and mechanistic studies within nasal drug delivery research.
Wadell
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Pharmacia and Upjohn, Stockholm, Sweden. cecilia.wadell@arcus.se.astra.com
Björk
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Camber
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D000281:Administration, Intranasal; D000818:Animals; D066298:In Vitro Techniques; D008954:Models, Biological; D009297:Nasal Mucosa / Q000378:metabolism*; D013552:Swine