title: A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E.
abstract: The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1, 616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched beta-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.
authors.affiliation: Tohoku University Gene Research Center, Sendai, 981-8555, Japan.
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: Y
authors.initials: Y
authors.lastname: Inagaki
authors.forename: T
authors.initials: T
authors.lastname: Suzuki
authors.forename: R X
authors.initials: RX
authors.lastname: Ioka
authors.forename: S Z
authors.initials: SZ
authors.lastname: Yoshioka
authors.forename: K
authors.initials: K
authors.lastname: Magoori
authors.forename: M J
authors.initials: MJ
authors.lastname: Kang
authors.forename: Y
authors.initials: Y
authors.lastname: Cho
authors.forename: A Z
authors.initials: AZ
authors.lastname: Nakano
authors.forename: Q
authors.initials: Q
authors.lastname: Liu
authors.forename: T
authors.initials: T
authors.lastname: Fujino
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: H
authors.initials: H
authors.lastname: Sasano
authors.forename: T T
authors.initials: TT
authors.lastname: Yamamoto
mesh_terms: D000302:Adrenal Cortex / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism; D001665:Binding Sites; D015152:Blotting, Northern; D002784:Cholesterol / Q000378:metabolism; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D026502:LDL-Receptor Related Proteins; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001057:Apolipoproteins E; D026502:LDL-Receptor Related Proteins; C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D011973:Receptors, LDL; D002784:Cholesterol