Staging of middle and late embryonic development in the medicinal leech, Hirudo medicinalis.
We present a description of the last half of embryonic development in the European medicinal leech, Hirudo medicinalis, based entirely on externally visible morphological features, and establish reliably observable stages during that development. Embryogenesis, from the time fertilized eggs are deposited in an eggcase (called a cocoon) to the emergence of juveniles from the cocoon, takes approximately 4 weeks at room temperature. The stages described in this paper extend from the completion of segmentation to the appearance of the final bands of pigmentation. Developmental stages are expressed as percentages of total embryonic developmental time. This staging table was constructed for embryos kept at 20 degrees C. In addition, the development of animals kept at 17 degrees C or at 24 degrees C was compared with those held at 20 degrees C. Development proceeds more quickly at higher temperatures. Because development in embryos held at higher or lower temperatures was linearly related to the stages determined for embryos held at 20 degrees C, the rate of development at any intermediate temperature can be predicted from the staging table at 20 degrees C by simple multiplication.
Reynolds
S A
SA
Department of Biology, University of California-San Diego, La Jolla 92093-0357, USA.
French
K A
KA
Baader
A
A
Kristan
W B
WB
D000818:Animals; D047108:Embryonic Development; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development; D007865:Leeches / Q000033:anatomy & histology / Q000196:embryology* / Q000254:growth & development; D009024:Morphogenesis; D010858:Pigmentation; D013696:Temperature; D013997:Time Factors