Exogenous recombinant human IL-12 augments MHC class I antigen expression on human cancer cells in vitro.
We investigated whether expressions of MHC class I and class II antigens relevant to tumor antigen presentation were changed on human tumor cells cultured with or without recombinant human IL-12(rhIL-12). We showed that the expression of MHC class I antigen on UTC-8, 28-1Cl and SBC-3 cells was augmented when these cancer cells were cultured with rhIL-12. The expression of class II antigen was slightly raised on UTC-8 and 28-1Cl cells by rhIL-12, but not enhanced on SBC-3 cells. These results suggest that rhIL-12 may provide possible enhancement of immunologic tumor recognition, and cytotoxic activity of lymphocytes against tumors through the enhanced expression of MHC class I antigen.
Suzuki
S
S
Department of Respiratory Oncology and Molecular Medicine, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Umezu
Y
Y
Saijo
Y
Y
Satoh
G
G
Abe
Y
Y
Satoh
K
K
Nukiwa
T
T
D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D015395:Histocompatibility Antigens Class I / Q000032:analysis*; D006801:Humans; D018664:Interleukin-12 / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D009062:Mouth Neoplasms / Q000276:immunology* / Q000473:pathology; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; D011994:Recombinant Proteins; D018664:Interleukin-12