Biodegradation and tumorigenicity of implanted plates made from a copolymer of epsilon-caprolactone and L-lactide in rat.
Flat plates made from a copolymer of epsilon-caprolactone and L-lactide (P-CL-LA) [50:50 (w/w), molecular weight 1.62 x 10(5); 20 x 10 x 1 mm size] were subcutaneously implanted into 50 young, male Wistar rats (P-CL-LA group). After 24 months the plates had become a mass of small pieces, which were concentrated in an area of 3 x 2 x 1 mm. For comparison, 50 rats were implanted with medical-grade polyethylene plates (PE group) while another set of 50 rats was subjected to the same operation but without an implant (Sham Op group). Tumors arose in 25 rats from the P-CL-LA group: 24 were malignant mesenchymal tumors at the implant sites. In the PE group, tumors appeared in 16 rats (14 at the implant sites and two ectopically). The average tumor latency was 578+/-84 days in the P-CL-LA group and 452+/-102 days in the PE group. There was no difference in tumor incidence between the P-CL-LA and PE groups (p < 0.05). In the Sham Op group, two malignant tumors appeared over 2 years. Pathologically, these induced tumors arose from the inflammatory cells surrounding the degrading fragments of P-CL-LA within the tissue capsule. This indicates that relatively slowly degrading material can induce malignant tumors at a similarly high rate to nonabsorbable medical grade PE, at least in this animal model.
Nakamura
T
T
Department of Physiological Artificial Organs, Research Center for Biomedical Engineering, Kyoto University, Japan.
Shimizu
Y
Y
Takimoto
Y
Y
Tsuda
T
T
Li
Y H
YH
Kiyotani
T
T
Teramachi
M
M
Hyon
S H
SH
Ikada
Y
Y
Nishiya
K
K
D000008:Abdominal Neoplasms / Q000209:etiology / Q000473:pathology; D000818:Animals; D001672:Biocompatible Materials / Q000493:pharmacokinetics* / Q000633:toxicity*; D001673:Biodegradation, Environmental; D001835:Body Weight; D002294:Carcinoma, Squamous Cell / Q000209:etiology / Q000473:pathology; D005354:Fibrosarcoma / Q000209:etiology / Q000473:pathology; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology; D012516:Osteosarcoma / Q000209:etiology / Q000473:pathology; D011091:Polyesters / Q000493:pharmacokinetics* / Q000633:toxicity*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D001672:Biocompatible Materials; D011091:Polyesters; C062968:lactide-caprolactone copolymer