title: Anti-complement activity of tiliroside from the flower buds of Magnolia fargesii.
abstract: As part of the search for anticomplementary active components from natural products, the anticomplementary properties of methanolic extracts from the flower buds of Magnoliafargesii have been investigated. Bioassay-guided chromatographic separation of the active constituents led to the isolation of compound 1, whose structure was identified by spectroscopic methods to be kaempferol 3-O-beta-D-(6"-O-coumaroyl)glucopyranoside (tiliroside). Tiliroside showed very potent anti-complement activity (IC50=5.4 x 10(-5) M) on the classical pathway of the complement system, even higher than rosmarinic acid, which is a well-known inhibitor against the complement system. On the other hand, the hydrolysates of tiliroside, kaempferol, astragalin and p-coumaric acid showed very weak activity on this system.
authors.affiliation: Natural Product Biosynthesis Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon.
authors.forename: K Y
authors.initials: KY
authors.lastname: Jung
authors.forename: S R
authors.initials: SR
authors.lastname: Oh
authors.forename: S H
authors.initials: SH
authors.lastname: Park
authors.forename: I S
authors.initials: IS
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Ahn
authors.forename: J J
authors.initials: JJ
authors.lastname: Lee
authors.forename: H K
authors.initials: HK
authors.lastname: Lee
mesh_terms: D001578:Benzopyrans / Q000302:isolation & purification* / Q000494:pharmacology*; D003169:Complement Inactivator Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects; D005419:Flavonoids; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
chemical_list: D001578:Benzopyrans; D003169:Complement Inactivator Proteins; D005419:Flavonoids; D010936:Plant Extracts; C052083:tiliroside