title: The effect of overnight storage of leukapheresis stem cell products on cell viability, recovery, and cost.
abstract: The cost of harvesting, processing, and freezing multiple peripheral blood stem cell (PBSC) products could easily exceed that of bone marrow harvest. To reduce costs while maintaining product viability, we examined the effect of overnight storage on PBSC products. Sixteen consecutive leukapheresis samples from 12 patients were examined prospectively. Each initial leukapheresis product was stored overnight on ice (median temperature 15 degrees C) after adding an equal amount of M199 culture medium containing heparin. After overnight storage, the product was combined with the next day PBSC harvest if required and processed/frozen per protocols. Parameters measured before and after storage include cell count and differential, viability, bacterial cultures, and colony-forming unit (CFU) assays. The results show that the median cell concentration during storage was 7.12 x 10(7)/ml and the median length of storage was 20 h. After storage, the median viability and nucleated cell recovery were 100% and 99.5%, respectively. In addition, 98% recovery of CFU-GM was achieved. No clotting or bacterial contamination was detected. All 12 patients studied engrafted promptly. In addition, 124 similarly treated patients were retrospectively analyzed. Of these, 48% required > or = 2 large-volume leukaphereses to achieve the target cell dose. As a result of overnight storage, 150 final products, instead of 224, were processed and cryopreserved. This difference is equivalent to 33% cost savings. Again, all patients were transplanted and engrafted successfully. In conclusion, overnight storage and pooling of two consecutive PBSC products are safe, reduce cost, and allow for optimum laboratory staffing.
authors.affiliation: Stem Cell Laboratory, Shands Teaching Hospital and Clinics, Gainesville, FL 32610-0277, USA.
authors.forename: M W
authors.initials: MW
authors.lastname: Sugrue
authors.forename: C E
authors.initials: CE
authors.lastname: Hutcheson
authors.forename: D D
authors.initials: DD
authors.lastname: Fisk
authors.forename: C G
authors.initials: CG
authors.lastname: Roberts
authors.forename: A
authors.initials: A
authors.lastname: Mageed
authors.forename: J R
authors.initials: JR
authors.lastname: Wingard
authors.forename: J S
authors.initials: JS
authors.lastname: Moreb
mesh_terms: D000293:Adolescent; D000328:Adult; D001793:Blood Preservation; D002470:Cell Survival; D002648:Child; D003365:Costs and Cost Analysis; D006085:Graft Survival; D019650:Hematopoietic Stem Cell Mobilization / Q000191:economics / Q000379:methods; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008875:Middle Aged; D014182:Transplantation, Autologous