Pancreatic dysfunction and its association with fat malabsorption in HIV infected children.
BACKGROUND
Nutrient malabsorption frequently occurs in HIV infected children, but very few studies have investigated exocrine pancreatic digestive capacity in these cases.


OBJECTIVE
To investigate pancreatic function in HIV infected children and to determine whether faecal fat loss, a prominent feature of intestinal dysfunction, is associated with pancreatic dysfunction.


METHODS
Forty seven children with HIV infection without apparent pancreatic disease and 45 sex and age matched healthy controls.


METHODS
Pancreatic function was evaluated by measuring elastase 1 concentration and chymotrypsin activity in stools by ELISA and colorimetric methods, respectively. Intestinal function was evaluated by measuring fat and protein loss by the steatocrit method and by faecal alpha1 antitrypsin concentration.


RESULTS
14 (30%) had abnormal pancreatic function tests: seven had isolated elastase activity deficiency, three isolated chymotrypsin deficiency, and four pancreatic deficiencies in both enzymes. Patient enzyme values were significantly lower than those of controls. Low faecal pancreatic enzymes were not associated with symptoms. Twelve children had steatorrhoea and four had increased alpha1 antitrypsin. Steatorrhoea was significantly associated with reduced faecal pancreatic enzymes. There was a significant negative correlation between elastase 1 concentration and steatocrit. Children with pathological faecal elastase 1 or chymotrypsin values did not differ from the other HIV infected children with respect to nutritional and immunological status, stage of HIV disease, presence of opportunistic infections, or drug administration.


CONCLUSIONS
Abnormal pancreatic function tests are a frequent feature of paediatric HIV infection; this condition is associated with steatorrhoea, which probably contributes to the disease.
Carroccio
A
A
Department of Internal Medicine, University of Palermo, Palermo, Italy.
Fontana
M
M
Spagnuolo
M I
MI
Zuin
G
G
Montalto
G
G
Canani
R B
RB
Verghi
F
F
Di Martino
D
D
Bastoni
K
K
Buffardi
F
F
Guarino
A
A
D000293:Adolescent; D016022:Case-Control Studies; D002446:Celiac Disease / Q000150:complications / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D002918:Chymotrypsin / Q000032:analysis; D004041:Dietary Fats / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D005260:Female; D015658:HIV Infections / Q000150:complications* / Q000378:metabolism; D006801:Humans; D007223:Infant; D007408:Intestinal Absorption / Q000502:physiology; D008286:Malabsorption Syndromes / Q000150:complications* / Q000378:metabolism; D008297:Male; D010182:Pancreatic Diseases / Q000150:complications* / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism; D011446:Prospective Studies
D004041:Dietary Fats; D002918:Chymotrypsin; D010196:Pancreatic Elastase