title: Pig heart fumarase contains two distinct substrate-binding sites differing in affinity.
abstract: A eukaryotic fumarase is for the first time unequivocally shown to contain two distinct substrate-binding sites. Pig heart fumarase is a tetrameric enzyme consisting of four identical subunits of 50 kDa each. Besides the true substrates L-malate and fumarate, the active sites (sites A) also bind their analogs D-malate and oxaloacetate, as well as the competitive inhibitor glycine. The additional binding sites (sites B) on the other hand also bind the substrates and their analogs D-malate and oxaloacetate, as well as L-aspartate which is not an inhibitor. Depending on the pH, the affinity of sites B for ligands (Kd being in the millimolar range) is 1-2 orders of magnitude lower than the affinity of sites A (of which Kd is in the micromolar range). However, saturating sites B results in an increase in the overall activity of the enzyme. The benzenetetracarboxyl compound pyromellitic acid displays very special properties. One molecule of this ligand is indeed able to bind into a site A and a site B at the same time. Four molecules of pyromellitic acid were found to bind per molecule fumarase, and the affinity of the enzyme for this ligand is very high (Kd = 0.6 to 2.2 microM, depending on the pH). Experiments with this ligand turned out to be crucial in order to explain the results obtained. An essential tyrosine residue is found to be located in site A, whereas an essential methionine residue resides in or near site B. Upon limited proteolysis, a peptide of about 4 kDa is initially removed, probably at the C-terminal side; this degradation results in inactivation of the enzyme. Small local conformational changes in the enzyme are picked up by circular dichroism measurements in the near-UV region. This spectrum is built up of two tryptophanyl triplets, the first one of which is modified upon saturating the active sites (A), and the second one upon saturating the low affinity binding sites (B).
authors.affiliation: Department of Protein Chemistry, Vrije Universiteit Brussel, Institute of Molecular Biology and Biotechnology, Paardenstraat 65, B-1640 Sint-Genesius-Rode, Belgium. sbeeckma@vub.ac.be
authors.forename: S
authors.initials: S
authors.lastname: Beeckmans
authors.forename: E
authors.initials: E
authors.lastname: Van Driessche
mesh_terms: D000818:Animals; D020134:Catalytic Domain; D002942:Circular Dichroism; D005649:Fumarate Hydratase / Q000737:chemistry* / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D008293:Malates / Q000378:metabolism; D008958:Models, Molecular; D008970:Molecular Weight; D009206:Myocardium / Q000378:metabolism*; D011489:Protein Denaturation; D013053:Spectrophotometry; D013552:Swine; D014508:Urea
chemical_list: D005650:Fumarates; D008024:Ligands; D046911:Macromolecular Substances; D008293:Malates; C030298:malic acid; C032005:fumaric acid; D014508:Urea; D005649:Fumarate Hydratase