Changes in the content of rat epididymal fluid induced by prolonged treatment with tamoxifen.
Prolonged treatment with tamoxifen induces changes in the male reproductive tract in rats. In this study changes in the protein content of the rat epididymal fluid as a consequence of prolonged treatment with tamoxifen are reported. Among five lysosomal enzymes measured in the epididymal fluid, alpha-mannosidase (alpha-MAN) significantly diminished, but other enzymes did not. Electrophoretic analysis of fluids showed that proteins of estimated molecular weight 25, 60, 80-85 and 180 kDa decreased in the treated rats. We also detected an increase in the binding of beta-galactosidase (beta-GAL) to caudal spermatozoa in treated rats. These changes may be related in part to the loss of fertilizing capacity of spermatozoa after tamoxifen treatment.
Belmonte
S
S
Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
Maturano
M
M
Bertini
M F
MF
Pusiol
E
E
Sartor
T
T
Sosa
M A
MA
D000818:Animals; D001826:Body Fluids / Q000201:enzymology*; D004822:Epididymis / Q000033:anatomy & histology / Q000201:enzymology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008361:Mannosidases / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011467:Prostate / Q000033:anatomy & histology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013094:Spermatozoa / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology*; D013739:Testosterone / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism
D004965:Estrogen Antagonists; D011506:Proteins; D013629:Tamoxifen; D013739:Testosterone; D008361:Mannosidases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase