Pb(II)-translocating P-type ATPases.
The cad operon of Staphylococcus aureus plasmid pI258, which confers cadmium resistance, encodes a transcriptional regulator, CadC, and CadA, an ATP-coupled Cd(II) pump that is a member of the superfamily of cation-translocating P-type ATPases. The Escherichia coli homologue of CadA, termed ZntA, is a Zn(II)/Cd(II) pump. The results described in this paper support the hypothesis that ZntA and CadA are Pb(II) pumps. First, CadC is a metal-responsive repressor that responds to soft metals in the order Pb>Cd>Zn. Second, both CadA and ZntA confer resistance to Pb(II). Third, transport of 65Zn(II) in everted membrane vesicles of E. coli catalyzed by either of these two P-type ATPase superfamily members is inhibited by Pb(II).
Rensing
C
C
Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA.
Sun
Y
Y
Mitra
B
B
Rosen
B P
BP
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism; D001692:Biological Transport; D002104:Cadmium / Q000378:metabolism; D002384:Catalysis; D007854:Lead / Q000378:metabolism*; D013211:Staphylococcus aureus / Q000378:metabolism; D015032:Zinc / Q000378:metabolism
D001426:Bacterial Proteins; C080050:CadC protein, Bacteria; D002104:Cadmium; D007854:Lead; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C109898:Zn(II)-translocating P-type ATPase; C059771:cadmium translocating ATPase; D015032:Zinc