The postnatal emergence of dehydration anorexia in rats is temporally associated with the emergence of dehydration-induced inhibition of gastric emptying.
Osmotic dehydration produced by systemic hypertonic NaCl (HS) inhibits gastric motility and emptying and also inhibits feeding in adult rats. Conversely, in neonatal rats, dehydration does not inhibit feeding. The present study examined whether the postnatal emergence of dehydration anorexia is temporally associated with the emergence of dehydration-induced inhibition of gastric emptying. Rat pups 4 to 19 days old were injected subcutaneously with HS (0.75 M NaCl; 200 microL/10 g body weight). Control rats were injected with isotonic saline (0.15 M NaCl). Thirty minutes later, rats were given access to milk that could be lapped from paper towels on the floor of a warm testing chamber. Other HS-treated and control rats were given an intragastric load of 0.15 M NaCl (2% body weight) and then killed after 30 min to determine how much of the load had emptied from the stomach. Consistent with previous reports, HS-treated rats consumed significantly more milk than control rats from postnatal Day 4 (P4) through P11 but consumed significantly less milk than controls at P19. HS treatment did not affect gastric emptying of 0.15 M NaCl at P4 or P11. Conversely, HS treatment significantly inhibited gastric emptying at P19. These findings suggest that the hypophagic effects of dehydration develop in tandem with inhibitory effects on gastric motility and are consistent with the view that the full complement of mature homeostatic responses to plasma hyperosmolality requires coordinated activation of forebrain and hindbrain neural circuits that are only partially formed in neonatal rats.
Callahan
J B
JB
Department of Neuroscience, University of Pittsburgh, Pennsylvania 15260, USA.
Rinaman
L
L
D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn / Q000502:physiology*; D000855:Anorexia / Q000523:psychology*; D001798:Blood Proteins / Q000378:metabolism; D003681:Dehydration / Q000503:physiopathology* / Q000523:psychology*; D004435:Eating; D005260:Female; D005746:Gastric Emptying / Q000502:physiology*; D009994:Osmolar Concentration; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000097:blood
D001798:Blood Proteins; D012964:Sodium