Development of a transformation system for the multinuclear yeast Dipodascus (Endomyces) magnusii.
We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii. The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non-transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation.
Adamíková
L
L
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Griac
P
P
Tomáska
L
L
Nosek
J
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D001761:Bleomycin / Q000494:pharmacology; D015139:Blotting, Southern; D004261:DNA Replication / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D018274:Electroporation / Q000379:methods; D005819:Genetic Markers / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics; D004718:Saccharomycetales / Q000235:genetics*; D014170:Transformation, Genetic
D000900:Anti-Bacterial Agents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D005819:Genetic Markers; D001761:Bleomycin; C105427:Zeocin