Spi-1/PU.1 proto-oncogene induces opposite effects on monocytic and erythroid differentiation of K562 cells.
Spi-1/PU.1 is a hematopoietic transcription factor of the Ets family. To analyze the effects of ectopic expression of spi-1 on the proliferation/differentiation of human myeloid leukemia cells, K562 cells were stably transfected with a spi-1 expression vector. The transfected cell lines expressed elevated levels of spi-1 mRNA and protein and high Spi-1-DNA binding activity. The spi-1 transfected cells showed reduced growth rates and reduced clonogenic cell growth. When the erythroid and monocytic differentiation markers were analyzed, spi-1 overexpression resulted in opposite effects: erythroid differentiation was significantly inhibited in spi-1 transfectants, while spi-1 overexpression increased the monocytic differentiation of cells. These results indicate a differential role of Spi-1 on the differentiation of human myeloid leukemia cells.
Delgado
M D
MD
Grupo de Biología Molecular del Cáncer, Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, 39011, Spain.
Gutiérrez
P
P
Richard
C
C
Cuadrado
M A
MA
Moreau-Gachelin
F
F
León
J
J
D015703:Antigens, CD / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D004920:Erythropoiesis / Q000187:drug effects / Q000235:genetics*; D005822:Genetic Vectors; D006410:Hematopoiesis / Q000187:drug effects / Q000235:genetics* / Q000276:immunology; D006801:Humans; D020014:K562 Cells; D009000:Monocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins / Q000235:genetics* / Q000378:metabolism; D011519:Proto-Oncogenes; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014746:Vimentin / Q000096:biosynthesis; D000099232:Proto-Oncogene Protein Spi-1
D015703:Antigens, CD; D000090063:Proto-Oncogene Mas; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate; D015534:Trans-Activators; D014746:Vimentin; D000099232:Proto-Oncogene Protein Spi-1; C000715470:MAS1 protein, human