title: Changes in neurotensin mRNA by estrogen in the female rat preoptic area during aging: an in situ hybridization histochemistry study.
abstract: The present study examined changes in the response of neurotensin mRNA to estrogen during aging at the single cell level. Ten days after ovariectomy, 3-, 10-, and 15-month-old female rats were implanted with estrogen or cholesterol and sacrificed 4 days later. An in situ hybridization study revealed that estrogen significantly increased the number of cells expressing neurotensin mRNA in the preoptic area of all age groups. Furthermore, frequency analysis indicated that estrogen significantly increased the proportion of heavily labeled cells in older rats but not in younger rats. Distributions of the grains/cells between cholesterol- and estrogen-treated rats suggested that older rats were at least as responsive to estrogen as young rats and possibly even more responsive. The result suggests that, at least as reflected by neurotensin mRNA, reproductive senescence in rats is not due to a general decrease in sensitivity to estrogen. Indeed, there is evidence of an increased responsiveness to estrogen with age.
authors.affiliation: Department of Physiology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-Ku, Yokohama, Japan. toshiya@med.yokohama-cu.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Funabashi
authors.forename: S P
authors.initials: SP
authors.lastname: Kleopoulos
authors.forename: F
authors.initials: F
authors.lastname: Kimura
authors.forename: C V
authors.initials: CV
authors.lastname: Mobbs
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D002452:Cell Count; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D017403:In Situ Hybridization; D009496:Neurotensin / Q000096:biosynthesis*; D010052:Ovariectomy; D011301:Preoptic Area / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D004967:Estrogens; D012333:RNA, Messenger; D009496:Neurotensin