title: Crystal structure of deoxy-human hemoglobin beta6 Glu --> Trp. Implications for the structure and formation of the sickle cell fiber.
abstract: An atomic-level understanding of the interactions between hemoglobin molecules that contribute to the formation of pathological fibers in sickle cell disease remains elusive. By exploring crystal structures of mutant hemoglobins with altered polymerization properties, insight can be gained into sickle cell hemoglobin (HbS) polymerization. We present here the 2.0-A resolution deoxy crystal structure of human hemoglobin mutated to tryptophan at the beta6 position, the site of the glutamate --> valine mutation in HbS. Unlike leucine and isoleucine, which promote polymerization relative to HbS, tryptophan inhibits polymerization. Our results provide explanations for the altered polymerization properties and reveal a fundamentally different double strand that may provide a model for interactions within a fiber and/or interactions leading to heterogeneous nucleation.
authors.affiliation: Department of Biochemistry and Molecular Biology, Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
authors.forename: D J
authors.initials: DJ
authors.lastname: Harrington
authors.forename: K
authors.initials: K
authors.lastname: Adachi
authors.forename: W E
authors.initials: WE
authors.lastname: Royer
mesh_terms: D019943:Amino Acid Substitution; D018360:Crystallography, X-Ray; D018698:Glutamic Acid / Q000737:chemistry*; D006451:Hemoglobin, Sickle / Q000737:chemistry*; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D014364:Tryptophan / Q000737:chemistry*
chemical_list: D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D018698:Glutamic Acid; D014364:Tryptophan; C100003:deoxyhemoglobin