Identification of the rat homologue of the mouse capsulin gene by cDNA representational difference analysis.
Representational difference analysis of cDNA (cDNA RDA) is a PCR-based differential cloning method. We have found that this PCR-based subtraction technique does not have any bias towards smaller DpnII-generated fragments. We have successfully used this method to identify the rat homologue of the mouse capsulin gene.
Familari
M
M
The University of Melbourne, Department of Medicine at Western Hospital, Footscray, VIC 3011, Australia.m.familari@medicine.unimelb.e du.au
Giraud
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AS
D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D051379:Mice; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D051381:Rats; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D014157:Transcription Factors / Q000235:genetics*
D051792:Basic Helix-Loop-Helix Proteins; D018076:DNA, Complementary; D014157:Transcription Factors; C112923:Tcf21 protein, mouse; C491824:Tcf21 protein, rat