Band broadening during dc and field inversion capillary electrophoresis of lambda DNA in dilute hydroxyethylcellulose solutions.
Capillary electrophoresis of lambda double-stranded (ds) DNA (48.5 kbp) in dilute hydroxyethylcellulose (HEC) solutions shows band spreading that cannot be explained by diffusion alone. Dispersion and asymmetry factors of lambda ds-DNA bands were measured as functions of capillary length, HEC concentration and field strength. Band spreading and asymmetry can be explained by a recently developed model in which the dominant contribution is assumed to be dispersion in DNA-HEC disentanglement times. Bandwidth reduction using square-wave field inversion was also investigated. It is proposed that correlation of DNA motion is the source of band narrowing during field inversion.
Schwinefus
J J
JJ
Department of Chemistry, University of Michigan, Ann Arbor 48109, USA.
Morris
M D
MD
D010582:Bacteriophage lambda / Q000235:genetics*; D002482:Cellulose / Q000031:analogs & derivatives; D004247:DNA / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D008957:Models, Genetic
D004279:DNA, Viral; D002482:Cellulose; C002283:hydroxyethylcellulose; D004247:DNA