Visualization of serum albumin on electrophoretic gels using the specific ligand bilirubin.
Serum albumin, when incubated with bilirubin prior to electrophoresis, was visualized as a yellow-colored band during the electrophoretic run and did not require any staining. Furthermore, free bilirubin served as a tracking dye. A minimum of 20 micrograms of protein was detected very well by this method. The formation of a bilirubin-albumin complex did not affect the electrophoretic mobility as the protein complexed with bilirubin as well as free albumin moved with the same mobility. Only a single protein band was visualized by this method after electrophoresis of human plasma. The method is simple, less time-consuming and may be used in identifying bilirubin-binding proteins in various biological samples.
Khan
M M
MM
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Uttar Pradesh, India.
Muzammil
S
S
Kumar
Y
Y
Tayyab
S
S
D000818:Animals; D001663:Bilirubin; D001797:Blood Protein Electrophoresis / Q000379:methods*; D002352:Carrier Proteins / Q000097:blood / Q000302:isolation & purification; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D008024:Ligands; D012709:Serum Albumin / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D002352:Carrier Proteins; D008024:Ligands; D012709:Serum Albumin; C029783:bilirubin binding protein, human; D012710:Serum Albumin, Bovine; D001663:Bilirubin