Surface investigations on the development of a direct optical immunosensor.
In the present paper surface studies for the development of a direct optical immunosensor for fast diagnosis of a myocardial infarction are presented. A fatty acid binding protein was detected by monoclonal antibodies. The applied measuring system was the grating coupler BIOS-1. Based on commercially available transducer materials protein immobilisation techniques have been developed and characterised by TOF-SIMS, AFM and EM. Three different label-free assay types were investigated. Only one assay leads to a sensitive and regenerable sensor set-up. It was possible to detect concentrations of the fatty acid binding protein down to 330 ng/ml. The general applicability of a direct optical immunosensor in the field of myocardial infarction diagnosis was demonstrated by this.
Kröger
D
D
Institut für Chemo- und Biosensorik, Münster, Germany.
Katerkamp
A
A
Renneberg
R
R
Cammann
K
K
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000032:analysis; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D018625:Microscopy, Atomic Force; D018996:Myelin P2 Protein / Q000032:analysis; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000378:metabolism; D009363:Neoplasm Proteins; D055095:Optics and Photonics; D014159:Transducers; D025521:Tumor Suppressor Proteins
D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; C107608:FABP7 protein, human; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D025521:Tumor Suppressor Proteins