title: The effect of dried tomato, grape and apple pomace on the cholesterol metabolism and antioxidative enzymatic system in rats with hypercholesterolemia.
abstract: The effect of dried tomato, grape and apple pomace (5% in diet) on the cholesterol levels in serum and selected organs was studied in male Wistar rats fed a cholesterol diet (0.3%) shortly after weaning. Pomace of individual fruits contained 47.2, 67.0 and 46.2 g of total dietary fiber per 100 g of dry matter for tomato, grape and apple pomace, respectively. Corresponding values for the soluble components of the fiber were 10.3, 14.7 and 26.0%. None of the tested products affected the serum cholesterol levels. Tomato and apple pomace significantly reduced cholesterol level in liver by 15% and 11%, respectively, and all tested pomace species reduced cholesterol content in heart (by 18-21%). Triacylglycerol content was affected only by tomato pomace and solely in the heart. Cholesterol absorption measured by dual-isotope ratio method, was not significantly affected by the pomace, however, all products strongly reduced (up to the half of the original value) the activity of HMG-CoA reductase in liver and increased the fractional catabolic rate of plasma cholesterol (by 60-120%). All tested pomace reduced plasma levels of conjugated dienes and tomato and grape pomace exhibited this effect also in the liver. All tested pomace reduced the activities of antioxidant enzymes superoxid dismutase, catalase and glutathione peroxidase in erythrocytes (by 34-56%).
authors.affiliation: Research Institute of Nutrition, Bratislava, Slovak Republic.
authors.forename: P
authors.initials: P
authors.lastname: Bobek
authors.forename: L
authors.initials: L
authors.lastname: Ozdín
authors.forename: M
authors.initials: M
authors.lastname: Hromadová
mesh_terms: D000821:Animal Feed; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002784:Cholesterol / Q000097:blood / Q000378:metabolism*; D004032:Diet; D004043:Dietary Fiber / Q000008:administration & dosage; D006937:Hypercholesterolemia / Q000201:enzymology / Q000378:metabolism*; D018551:Solanum lycopersicum; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019685:Rosales; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D004043:Dietary Fiber; D014280:Triglycerides; D002784:Cholesterol