title: Trichoderma/pathogen interactions: measurement of antagonistic chemicals produced at the antagonist/pathogen interface using a tubular bioassay.
abstract: A tubular bioassay was used to measure analytically the local production and concentration of the antifungal Trichoderma secondary metabolite 6-n-pentyl-2H-pyran-2-one (6PAP) at the Trichoderma antagonist/pathogen interface. 6PAP levels significantly increased in the presence of the pathogen Botrytis cinerea, typically 300-700%, and were highest near the pathogen source. The level of response for a particular Trichoderma isolate was found to vary with the test organism used. Two products produced by biotransformation of 6PAP by B. cinerea in response to the interaction were also detected.
authors.affiliation: HortResearch, Ruakura Research Centre, Hamilton, New Zealand. jcooney@hort.cri.nz
authors.forename: J M
authors.initials: JM
authors.lastname: Cooney
authors.forename: D R
authors.initials: DR
authors.lastname: Lauren
mesh_terms: D000898:Antibiosis / Q000502:physiology*; D000935:Antifungal Agents / Q000378:metabolism* / Q000494:pharmacology; D001681:Biological Assay / Q000379:methods*; D020171:Botrytis / Q000502:physiology; D005670:Fusarium / Q000502:physiology; D011753:Pyrones / Q000378:metabolism* / Q000494:pharmacology; D012232:Rhizoctonia / Q000502:physiology; D013172:Spores, Fungal / Q000254:growth & development; D013997:Time Factors; D014242:Trichoderma / Q000737:chemistry / Q000502:physiology*; D015003:Yeasts / Q000502:physiology
chemical_list: C107766:6-n-pentyl-2H-pyran-2-one; D000935:Antifungal Agents; D011753:Pyrones