Cell cycle arrest following exposure of EBV-immortalised B-cells to gamma irradiation correlates with inhibition of cdk2 activity.
The exposure of Epstein-Barr virus immortalised B cells (LCLs) to the genotoxic effects of gamma irradiation causes a decreased proliferation of the cells. The early events in this process have been investigated here. The induction of p53 expression correlates with a cell cycle arrest in the G1 and G2/M phases of the cell cycle within 24 h of exposure. The molecular mechanism governing the decreased proliferation appears to involve the induction of the cyclin dependent kinase (cdk) inhibitor p21CIP1 and its functional association with cdk2.
Cannell
E J
EJ
Ludwig Institute for Cancer Research, Imperial College School of Medicine at St. Mary's Hospital, London, UK.
Farrell
P J
PJ
Sinclair
A J
AJ
D017209:Apoptosis; D001402:B-Lymphocytes / Q000166:cytology / Q000201:enzymology / Q000528:radiation effects*; D042846:CDC2-CDC28 Kinases; D002453:Cell Cycle / Q000528:radiation effects*; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D051357:Cyclin-Dependent Kinase 2; D050759:Cyclin-Dependent Kinase Inhibitor p21; D018844:Cyclin-Dependent Kinases / Q000502:physiology*; D016213:Cyclins / Q000096:biosynthesis / Q000502:physiology; D004794:Enzyme Repression; D005720:Gamma Rays; D004854:Herpesvirus 4, Human / Q000502:physiology; D006801:Humans; D017346:Protein Serine-Threonine Kinases / Q000502:physiology*; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D016213:Cyclins; D016159:Tumor Suppressor Protein p53; D017346:Protein Serine-Threonine Kinases; D042846:CDC2-CDC28 Kinases; C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2; D018844:Cyclin-Dependent Kinases