Distribution of enzymes involved in the metabolism of glycyrrhizin in various organs of rat.
Glycyrrhizin (GL) was hydrolyzed to glycyrrhetic acid (GA), glycyrrhetic acid mono-beta-D-glucuronide (GAMG) or both by glucuronidases in various organs of rat. GL beta-D-glucuronidase I, hydrolyzing GL to GA; GAMG beta-D-glucuronidase, hydrolyzing GAMG to GA; and 3alpha-hydroxyglycyrrhetinate (3alpha-hydroxyGA) dehydrogenase, oxidizing 3alpha-hydroxyGA to 3-oxo-GA were found in the organs of this animal. GL beta-D-glucuronidase II was distributed in the lysosomal fraction of all organs except brain; 3alpha-hydroxyGA dehydrogenase was distributed in the microsomal fraction of the liver; but other enzymes were distributed in the nuclear, lysosomal, microsomal and soluble fractions of a variety of organs. GL beta-D-glucuronidase I, GL beta-D-glucuronidase II and GAMG beta-D-glucuronidase activities in a mixture of lysosomes and microsomes of rat liver exhibited different patterns on hydroxyapatite column chromatography. These results showed the metabolic pathways of GL to be of two types: a beta-D-glucuronidase hydrolyzing GL to GA, and the other consisting of two different beta-D-glucuronidases hydrolyzing GL to GAMG and GAMG to GA.
Akao
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Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
D015096:3-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002845:Chromatography / Q000379:methods; D017886:Durapatite; D005966:Glucuronidase / Q000378:metabolism*; D006034:Glycyrrhetinic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D017208:Rats, Wistar; D013347:Subcellular Fractions / Q000201:enzymology; D014018:Tissue Distribution
C052400:glycyrrhetyl 3-monoglucuronide; D019695:Glycyrrhizic Acid; D017886:Durapatite; D015096:3-Hydroxysteroid Dehydrogenases; D000429:Alcohol Oxidoreductases; C056636:3-alpha-hydroxyglycyrrhetinate dehydrogenase; C052783:glycyrrhizin beta-D-glucuronidase; D005966:Glucuronidase; D006034:Glycyrrhetinic Acid