Siderotyping of fluorescent pseudomonads: characterization of pyoverdines of Pseudomonas fluorescens and Pseudomonas putida strains from Antarctica.
Five independent fluorescent pseudomonad isolates originating from Antarctica were analysed for their pyoverdine systems. A pyoverdine-related siderotyping, which involved pyoverdine-induced growth stimulation, pyoverdine-mediated iron uptake, pyoverdine analysis by electrophoresis and isoelectric focusing, revealed three different pyoverdine-related siderotypes among the five isolates. One siderotype, including Pseudomonas fluorescens 1W and P. fluorescens 10CW, was identical to that of P. fluorescens ATCC 13525. Two other strains, P. fluorescens 9AW and Pseudomonas putida 9BW, showed identical pyoverdine-related behaviour to each other, whereas the fifth strain, P. fluorescens 51W, had unique features compared to the other strains or to a set of 12 fluorescent Pseudomonas strains used as comparison material. Elucidation of the structure of the pyoverdines produced by the Antarctic strains supported the accuracy of the siderotyping methodology by confirming that pyoverdines from strains 1W and 10CW had the same structures as the P. fluorescens ATCC 13525 pyoverdine, whereas the 9AW and 9BW pyoverdines are probably identical with the pyoverdine of P. fluorescens strain 244. Pyoverdine from strain 51W appeared to be a novel pyoverdine since its structure was different from all previously established pyoverdine structures. Together with the conclusion that the Antarctic Pseudomonas strains have no special features at the level of their pyoverdines and pyoverdine-mediated iron metabolism compared to worldwide strains, the present work demonstrates that siderotyping provides a rapid means of screening for novel pyoverdines.
Meyer
Jean-Marie
JM
Laboratoire de Microbiologie et de Gbnbtique, Universite Louis-Pasteur, UPRES A du CNRS no. 7010,28 rue Goethe, 67000 Strasbourg, France.
Stintzi
Alain
A
Laboratoire de Microbiologie et de Gbnbtique, Universite Louis-Pasteur, UPRES A du CNRS no. 7010,28 rue Goethe, 67000 Strasbourg, France.
Coulanges
Valie
V
Laboratoire de Microbiologie et de Gbnbtique, Universite Louis-Pasteur, UPRES A du CNRS no. 7010,28 rue Goethe, 67000 Strasbourg, France.
Shivaji
Sisinthy
S
Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
Voss
Jessica A
JA
lnstitut fur Organische Chemie, Universitat zu Kdn, Greinstrasse 4, D-50939 Kdn, Germany.
Taraz
Kambiz
K
lnstitut fur Organische Chemie, Universitat zu Kdn, Greinstrasse 4, D-50939 Kdn, Germany.
Budzikiewic
Herbert
H
lnstitut fur Organische Chemie, Universitat zu Kdn, Greinstrasse 4, D-50939 Kdn, Germany.
D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001692:Biological Transport; D005453:Fluorescence; D007501:Iron / Q000378:metabolism; D009842:Oligopeptides; D010860:Pigments, Biological / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000145:classification* / Q000254:growth & development / Q000378:metabolism; D016958:Pseudomonas putida / Q000145:classification* / Q000254:growth & development / Q000378:metabolism; D017262:Siderophores / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
D009842:Oligopeptides; D010860:Pigments, Biological; D017262:Siderophores; C042453:pyoverdin; D007501:Iron