Effect of airborne particles from selected indoor and outdoor environments on gap-junctional intercellular communication.
The effect of airborne particles from diesel exhaust, rubber and metal industry, urban air and biological sources (poultry, pig farming, compost industry) on gap-junctional intercellular communication (GJIC) were compared, using HEPA1c1c7 cells. Particles as such were compared with aqueous and organic extracts. Significant inhibition of GJIC by particle suspensions was only observed for the diesel and rubber samples, and for one biological sample (compost). Up to 83% of the inhibition of the whole suspension could be attributed to the particles as such. Washing the particles with organic solvents (aceton, methanol, hexane) did not result in a significant loss of activity from the particles, although the organic fractions showed a significant activity towards GJIC. More active organics was eluted from the rubber industry particles than from the diesel particles by the organic solvent. It is suggested that cancer promoting potential as measured by inhibition of GJIC may vary widely depending on the particle source, and that this effect may be exerted by the particles as such and/or by means of tightly bound bio-active material to the surface.
Alink
G M
GM
Department of Toxicology, Agricultural University, Wageningen, The Netherlands. Gerrit.Alink@Algemeen.tox.wau.nl
Sjögren
M
M
Bos
R P
RP
Doekes
G
G
Kromhout
H
H
Scheepers
P T
PT
D000383:Agriculture; D000393:Air Pollutants / Q000633:toxicity*; D016902:Air Pollution, Indoor; D000818:Animals; D002450:Cell Communication / Q000187:drug effects*; D017629:Gap Junctions / Q000187:drug effects*; D007221:Industry; D008099:Liver / Q000166:cytology / Q000187:drug effects; D008670:Metals; D051379:Mice; D010316:Particle Size; D012408:Rubber; D014407:Tumor Cells, Cultured; D001335:Vehicle Emissions
D000393:Air Pollutants; D008670:Metals; D001335:Vehicle Emissions; D012408:Rubber