Characterization of sera from subjects infected with HIV-1 subtypes B and E in Thailand by antibody binding and neutralization.
The range and specificity of the humoral immune response to HIV-1 subtypes B and E was investigated in Thai samples. Sera from HIV-1-positive subjects, consisting of subtypes B (n = 24) and E (n = 138), were characterized in relation to the neutralization of primary isolates and T-cell line-adapted (TCLA) strains and binding to monomeric gp120, the CD4/gp120 binding site (BS), and V3 peptides. A subtype-specific pattern of antibody binding was observed with the exception of the CD4/gp 120MN BS. Neutralization of TCLA strains (n = 4) was strongly type-specific (p = .002); however, neutralization of primary isolates (n = 8) was weak and group specific. Thus, the subtype specificity of B and E sera in the neutralization of TCLA strains, but not primary isolates, supports the dominance of the V3 region in TCLA virus neutralization but does not support the distinction of subtypes B and E as discrete neutralization serotypes in Thailand.
Louisirirotchanakul
S
S
Department of Genito-Urinary Medicine and Communicable Diseases, Imperial College School of Medicine at St Mary's, London, UK.
Beddows
S
S
Cheingsong-Popov
R
R
Shaffer
N
N
Mastro
T D
TD
Auewarakul
P
P
Likanonsakul
S
S
Wasi
C
C
Weber
J
J
D000595:Amino Acid Sequence; D000937:Antigen-Antibody Reactions; D015704:CD4 Antigens / Q000276:immunology; D002460:Cell Line; D016384:Consensus Sequence; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D015483:HIV Antibodies / Q000097:blood*; D015699:HIV Envelope Protein gp120 / Q000737:chemistry / Q000276:immunology*; D006679:HIV Seropositivity / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000145:classification* / Q000276:immunology* / Q000502:physiology; D006801:Humans; D019169:Jurkat Cells; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D013601:T-Lymphocytes; D013785:Thailand; D014779:Virus Replication
D015704:CD4 Antigens; D000939:Epitopes; D015483:HIV Antibodies; D015699:HIV Envelope Protein gp120