Mechanisms of anionic detergent-induced hemolysis.
The effect of osmotic protectors (sucrose and polyethylene glycols) and of a decrease in the detergent concentration at different points of hemolysis of human erythrocytes by sodium dodecyl sulphate on the shape of kinetic curves of hemolysis were studied. It is shown that slow detergent-induced hemolysis follows the colloid-osmotic mechanisms. Evidence is provided that rapid hemolysis by sodium dodecyl sulphate is caused by opening of large pores sufficient for the release of hemoglobin molecules rather than by the colloid-osmotic mechanism, and that the kinetics of hemolysis is mainly determined by time dependence of the opening probability of these pores.
Chernitsky
E
E
Institute of Photobiology, National Academy of Sciences of Belarus, Minsk, Belarus. sei@biobel.bas-net.by
Senkovich
O
O
D004912:Erythrocytes / Q000187:drug effects* / Q000502:physiology; D006461:Hemolysis / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D013395:Sucrose