Stimulatory effects of starfish sapogenins (Asterias amurensis and Lethasterias nanimensis chelifera) on molluscan heart (Spisula sachalinensis).
Sapogenins from the starfish Asterias amurensis and Lethasterias nanimensis chelifera, 5 alpha-pregn-9(11)-ene-3 beta,6 alpha-diol-20-one, 5 alpha-cholest-9(11)-ene-3 beta,6 alpha-diol-23-one, 5 alpha-cholesta-9(11),24(25)-diene-3 beta,6 alpha-diol-23-one, (20E)-5 alpha-cholesta-9(11),20(22)-diene-3 beta,6 alpha-diol-23-one and 24 zeta-methyl-5 alpha-cholesta-9(11),20(22)-diene-3 beta,6 alpha-diol-23-one, stimulated the contractile force of the heart of the mollusk Spisula sachalinensis at concentration of 5 x 10(-5) M. Ouabain, a specific inhibitor of Na+,K(+)-ATPase, at concentration of 5 x 10(-5) M had no effect on this physiological model. Starfish sapogenins of the cholestane series moderately inhibited rat brain cortex Na+,K(+)-ATPase and decreased Ca2+ influx into Ehrlich carcinoma cells. In contrast, pregnane asterogenin asterone did not inhibit Na+,K(+)-ATPase and increased the influx of Ca2+ into cells. These effects were not the result of cell membrane damage, because none of the compounds tested have hemolytic activity.
Gorshkov
B A
BA
Laboratory of the Chemistry of Marine Natural Products, Pacific Institute of Bioorganic Chemistry, Vladivostok, Russia.
Kapustina
I I
II
Kicha
A A
AA
Aminin
D L
DL
Gorshkova
I A
IA
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002783:Cholestenones / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006321:Heart / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D008974:Mollusca; D009200:Myocardial Contraction / Q000187:drug effects; D010042:Ouabain / Q000494:pharmacology; D011283:Pregnenes / Q000494:pharmacology; D051381:Rats; D012502:Sapogenins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D013215:Starfish; D013261:Sterols / Q000494:pharmacology; D014407:Tumor Cells, Cultured
D002783:Cholestenones; D004791:Enzyme Inhibitors; D011283:Pregnenes; D012502:Sapogenins; D012503:Saponins; D013261:Sterols; C007410:dihydromarthasterone; C008330:marthasterone; C012754:asterone; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium