Interleukin-12 expression in B cells by transformation with Epstein-Barr virus.
Although interleukin (IL)-12 was originally purified from an Epstein-Barr (EBV)-transformed B cell line and the high correlation of EBV infection and IL-12 expression has been suggested, no study has reported whether EBV infection is directly linked to IL-12 expression. To address this issue, we have investigated IL-12 expression in B cells during in vitro transformation with EBV. Human peripheral B cells became capable of constitutively producing p40 by in vitro transformation with EBV, coincident with the expression of latent membrane protein 1 (LMP1) of EBV. These B cells expressed p40 and p35 mRNA, and phorbol myristate acetate (PMA) stimulation strongly enhanced p40 and p70 production. Furthermore, transfection with LMP1 expression vector into a human B lymphoma cell line, Daudi, led to p40 production with nuclear factor (NF)-kappaB activation. These results suggest that transformation of primary B cells with EBV induces IL-12 expression potentially through LMP1 expression.
Yoshimoto
T
T
Institute of Medical Science, The University of Tokyo, Tokyo, 108-8639, Japan. yoshimot@ims.u-tokyo.ac.jp
Nagase
H
H
Yoneto
T
T
Inoue
J i
Ji
Nariuchi
H
H
D001402:B-Lymphocytes / Q000378:metabolism* / Q000821:virology; D002051:Burkitt Lymphoma / Q000378:metabolism; D002472:Cell Transformation, Viral; D006023:Glycoproteins / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D006801:Humans; D018664:Interleukin-12 / Q000096:biosynthesis*; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B / Q000494:pharmacology; D011487:Protein Conformation; D018121:Receptors, Cytokine; D014162:Transfection; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics
C097732:EBI3 protein, human; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D006023:Glycoproteins; D007378:Interleukins; D015778:Minor Histocompatibility Antigens; D016328:NF-kappa B; D018121:Receptors, Cytokine; D014763:Viral Matrix Proteins; D018664:Interleukin-12