title: A patient with myelodysplastic syndrome studied by GTG-banding and fluorescent in situ hybridization (FISH).
abstract: Molecular cytogenetics using fluorescent in situ hybridization (FISH) is an extremely useful adjunct technique to conventional cytogenetics via GTG-banding. The present paper illustrates the utility of FISH by describing a patient with myelodysplastic syndrome (MDS) who was initially studied using GTG-banding and whose bone marrow was found to be populated with hyperdiploid cells. FISH was used to delineate the numerical and structural chromosomal abnormalities. It revealed the presence of trisomy 8 and determined that the previously unidentifiable marker chromosome was of chromosome 10 origin. Although trisomy 8 is a frequent finding in MDS, the structural chromosomal abnormality of chromosome 10 as reported here is not a common finding.
authors.affiliation: Department of Pathology, Rhode Island Hospital, Providence 02903, USA.
authors.forename: H F
authors.initials: HF
authors.lastname: Mark
authors.forename: Y
authors.initials: Y
authors.lastname: Mark
authors.forename: E
authors.initials: E
authors.lastname: Sotomayor
authors.forename: S
authors.initials: S
authors.lastname: Sambandam
mesh_terms: D000368:Aged; D002871:Chromosome Banding; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008677:Metaphase; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D014178:Translocation, Genetic