cDNA cloning and characterization of a constitutively expressed isoform of the human peroxin Pex11p.
We cloned a human cDNA encoding an isoform of the peroxin Pex11p, termed Pex11pbeta, by screening of human liver cDNA library using as a probe human EST-derived, approximately 300 bp-long nucleotides showing homology to PEX11 from Candida boidinii and Saccharomyces cerevisiae. PEX11beta encoded a protein comprising 259 amino acids, with two putative transmembrane segments, showing approximately 40% identity to inducible Pex11palpha, at the amino acid sequence level. Pex11pbeta was found to be a peroxisomal protein, as assessed by colocalization with acyl-CoA oxidase, an enzyme catalyzing the first step of peroxisomal beta-oxidation system, in Pex11pbeta-expressing Chinese hamster ovary cells. PEX11beta was not induced in rats by treatment of clofibrate, a peroxisome proliferator, in contrast to PEX11alpha.
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Department of Biology, Faculty of Science, Kyushu University, Fukuoka, 812-8581, Japan.
Fujiki
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D003001:Cloning, Molecular; D006224:Cricetinae; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008830:Microbodies / Q000378:metabolism*; D008969:Molecular Sequence Data; D000074401:Peroxins; D016133:Polymerase Chain Reaction; D051381:Rats; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism
D017931:DNA Primers; D018076:DNA, Complementary; D008565:Membrane Proteins; C490455:PEX11A protein, human; C490453:PEX11B protein, human; D000074401:Peroxins; C490454:Pex11a protein, rat