cDNA sequence analysis and expression of four long neurotoxin homologues from Naja naja atra.
The novel cDNAs encoding four long neurotoxin homologues were firstly cloned from the venom gland of Naja naja atra by reverse transcriptase-polymerase chain reaction. Amino acid sequence comparison showed that they may come from two different evolutionary origins. The mature proteins were expressed as soluble fusion proteins in Escherichia coli and purified for immunoblotting. The results revealed that they displayed different immunochemical properties.
Qian
Y C
YC
Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, 500 Caobao Road, Shanghai 200233, PR China.
Fan
C Y
CY
Gong
Y
Y
Yang
S L
SL
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D003039:Cobra Neurotoxin Proteins / Q000096:biosynthesis / Q000235:genetics*; D018076:DNA, Complementary / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics
D003039:Cobra Neurotoxin Proteins; D018076:DNA, Complementary; D011498:Protein Precursors; D011993:Recombinant Fusion Proteins