Quantitative reverse transcription-PCR assay of the RNA component of human telomerase using the TaqMan fluorogenic detection system.
We established the validity of a quantitative reverse transcription (RT)-PCR assay for the RNA component of human telomerase (hTR), using the TaqMan fluorogenic detection system. Using this assay, we quantified hTR expression in two human pancreatic cancer cell lines, ASPC-1 and MIAPaCa-2. Our results indicated that hTR expression in MIAPaCa-2 was 1.99-fold higher than that in ASPC-1 cells. This TaqMan RT-PCR assay appears to be useful in determining the amount of hTR in clinical specimens.
Yajima
T
T
Department of Laboratory Diagnosis, University School of Medicine, Sapporo 060-8543, Japan.
Yagihashi
A
A
Kameshima
H
H
Kobayashi
D
D
Furuya
D
D
Hirata
K
K
Watanabe
N
N
D002460:Cell Line; D005453:Fluorescence; D005456:Fluorescent Dyes; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D010179:Pancreas / Q000166:cytology / Q000201:enzymology; D012313:RNA / Q000032:analysis*; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019914:Taq Polymerase; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics*
D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; D012313:RNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D019914:Taq Polymerase; D019098:Telomerase