Abnormal expression of brush-border membrane transporters in the duodenal mucosa of two patients with microvillus inclusion disease.
BACKGROUND
Microvillus inclusion disease is a congenital disorder characterized by secretory diarrhea. Patients demonstrate villus atrophy, loss of microvilli, and internalized inclusions of microvilli within the cytoplasm of small intestinal enterocytes. The exact molecular defect in these patients is not known. Two infants are described in this report with microvillus inclusion disease. Case 1 was a 3-month-old boy who developed secretory diarrhea shortly after birth. Case 2 was a 9-month-old boy who had abrupt onset diarrhea at 2 weeks of age resulting in weight loss and dehydration. Light microscopy revealed total villus atrophy with minimal crypt hyperplasia, and electron microscopic examination revealed variably shortened microvilli and cytoplasmic microvillus inclusions in both patients.


METHODS
Poly (A)+ RNA was purified from duodenal biopsies and RT-PCR reactions were performed. Normal human intestinal RNA was used as a positive control. Primers specific for human NHE-1, NHE-2, NHE-3 (2 sets), sodium-glucose transporter (SGLT1), and beta-actin were used.


RESULTS
Results showed that NHE-1 and beta-actin cDNAs amplified to similar levels in both patient and control samples. However, the expression of NHE-2 and SGLT1 was much higher in the control sample than in the patient samples. Additionally, NHE-3 mRNA was not detected in the patient samples using two sets of NHE-3 specific primers.


CONCLUSIONS
The patients with microvillus inclusion disease have defects in apical but not basolateral membrane transport systems, and these defects are related to the pathogenesis of the disease.
Michail
S
S
Joint Division of Pediatric Gastroenterology and Nutrition, The University of Nebraska Medical Center and Creighton University, Omaha, USA.
Collins
J F
JF
Xu
H
H
Kaufman
S
S
Vanderhoof
J
J
Ghishan
F K
FK
D003967:Diarrhea / Q000378:metabolism* / Q000473:pathology; D004386:Duodenum / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007223:Infant; D007413:Intestinal Mucosa / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008871:Microvilli / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D017923:Sodium-Hydrogen Exchangers