title: Apatite precipitation after incubation of biphasic calcium-phosphate ceramic in various solutions: influence of seed species and proteins.
abstract: The dissolution-precipitation process for calcium-phosphate ceramics in contact with biological fluid was studied by incubating blocks of biphasic calcium phosphate composed of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) in different solutions: ionic simulated body fluid (SBF) without protein or SBF that contained various proteins and macromolecules separately (fibronectin, vitronectin, albumin, and poly-L-glutamic acid). Transmission electron microscopy studies revealed that apatite-precipitated microcrystals appeared around ceramic crystals as a result of secondary nucleation; microcrystals were in continuity with the lattice planes of the HA crystals but in a different direction from that of beta-TCP; the size of the precipitates was smaller when fibronectin, vitronectin, and poly-(L-glutamic acid) were present in SBF as compared to SBF without protein; and fibronectin and vitronectin initiated crystal nucleation in the void spaces between the ceramic crystals.
authors.affiliation: Centre de Recherche Interdisciplinaire sur les Matériaux d'Intérêt Biologique, UPRES 2159, Nantes, France. rroh@sante.univ-nantes.fr
authors.forename: R
authors.initials: R
authors.lastname: Rohanizadeh
authors.forename: M
authors.initials: M
authors.lastname: Padrines
authors.forename: J M
authors.initials: JM
authors.lastname: Bouler
authors.forename: D
authors.initials: D
authors.lastname: Couchourel
authors.forename: Y
authors.initials: Y
authors.lastname: Fortun
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
mesh_terms: D001031:Apatites / Q000032:analysis*; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000502:physiology*; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry*; D005353:Fibronectins; D006801:Humans; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D011099:Polyglutamic Acid; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D019096:Vitronectin
chemical_list: D001031:Apatites; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D005353:Fibronectins; D011506:Proteins; D012709:Serum Albumin; D012996:Solutions; D019096:Vitronectin; D011099:Polyglutamic Acid