title: The influence of oral lipid loads on acylation stimulating protein (ASP) in healthy volunteers.
abstract: OBJECTIVE
  To examine the hypothesis that a sustained rise in plasma acylation stimulating protein (ASP, C3a desarg) accompanies the elevation in triacylglycerol that follows the ingestion of an oral fat load.
  
  
  METHODS
  Following an overnight fast, blood samples were obtained from healthy volunteers while fasting and 15 min, 1, 2, 4, 6 and 8 h following ingestion of: (i) a liquid meal, rich in dairy fat (eight subjects) and (ii) a semi-liquid meal, with higher total fat content and rich in polyunsaturated fat (six subjects).
  
  
  METHODS
  Four male and four female volunteers (age range: 22-51 y; body mass index (BMI): 17.9-26.9 kg/m2) received the first meal. Six subjects (age range: 32-60 y; BMI: 18.0-28.4 kg/m2), including three from the first study, received the second meal using the same protocol. ASP and C5a were measured by radioimmunoassay (RIA) and the complement proteins C3, factor B and C5 by radial immunodiffusion or nephelometry. Tumour necrosis factor (TNF)-alpha was measured by enhanced ELISA, and plasma cholesterol and triacylglycerol by an automated enzymatic method. The presence of chylomicrons was assessed in post-prandial plasma samples taken after the second meal.
  
  
  RESULTS
  There was no significant change in mean ASP concentration in either group at any time point, following ingestion of either meal. However, there was a significant positive linear trend in ASP following the second fat challenge (ANOVA; P < 0.05). There was also no change in complement proteins, plasma cholesterol or TNF-alpha. Plasma triacylglycerol rose significantly after the first and second meals (P < 0.05 and P < 0.001 at 2 h post-prandially); the mean maximum rise above the fasting level was 58 +/- 41% and 89 +/- 38% respectively (mean +/- s.d.). Chylomicrons were detected in samples taken from each subject after the second meal. Analysis of individual ASP data showed a sustained rise in one subject after the first meal and two subjects after the second meal. Substantial variation in ASP concentration was observed in samples taken in the first 2 h post-prandially.
  
  
  CONCLUSIONS
  There was no significant change in ASP nor other complement proteins for either group of subjects following ingestion of the lipid loads. Individual data showed substantial variation in post-prandial ASP, but multiple plasma sampling did not define the basis for this variation.
authors.affiliation: Department of Nephrology, Prince Henry Hospital, Little Bay, New South Wales, Australia.
authors.forename: J A
authors.initials: JA
authors.lastname: Charlesworth
authors.forename: P W
authors.initials: PW
authors.lastname: Peake
authors.forename: L V
authors.initials: LV
authors.lastname: Campbell
authors.forename: B A
authors.initials: BA
authors.lastname: Pussell
authors.forename: S
authors.initials: S
authors.lastname: O'Grady
authors.forename: T
authors.initials: T
authors.lastname: Tzilopoulos
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D001824:Body Constitution; D015992:Body Mass Index; D015926:Complement C3a / Q000031:analogs & derivatives*; D004041:Dietary Fats / Q000008:administration & dosage / Q000494:pharmacology*; D005215:Fasting; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D005260:Female; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D019518:Postprandial Period; D012016:Reference Values; D014280:Triglycerides / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis
chemical_list: D001798:Blood Proteins; D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D008055:Lipids; D014280:Triglycerides; D014409:Tumor Necrosis Factor-alpha; C044541:complement C3a, des-Arg-(77)-; D015926:Complement C3a