Anion-exchange high-performance liquid chromatographic assay of plasma lipoproteins of rabbits, rats and mice.
A fast, accurate and precise high-performance liquid chromatographic assay method has been developed for plasma lipoproteins of experimental animals, rabbits, rats and mice. The method includes complete separation of high, low and very low density lipoproteins from one another within 20 min by a DEAE-glucomannan gel using stepwise elution, and determination by postcolumn reaction with an enzymatic cholesterol reagent as the total cholesterol level. The relative standard deviation of each lipoprotein assay was highly reproducible, being less than 2.0 and 2.4% for repeatability and intermediate precision, respectively. The method was successfully applied to the assays of plasma lipoproteins in three species of normolipidemic and hyperlipidemic animals.
Yamaguchi
Y
Y
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Kunitomo
M
M
Haginaka
J
J
D000818:Animals; D000838:Anions; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D004983:Ethanolamines; D006949:Hyperlipidemias / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008351:Mannans; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000838:Anions; D004983:Ethanolamines; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008351:Mannans; C022901:(1-6)-alpha-glucomannan; D002784:Cholesterol; C007369:2-diethylaminoethanol