A fluorescent double stain for visualization of neural tissue by confocal laser scanning microscopy.
We present a fast and simple method for a general, fluorescent double stain that differentially labels various cellular components and visualizes all cells in confocal laser scanning microscopy. The technique is useful for two- and three-dimensional visualization of neural tissue and facilitates quantification of a variety of neuroanatomical parameters. Examples from cerebellum and retina are shown to demonstrate the broad applicability.
Kröger
R H
RH
Eberhard-Karls-Universität Tübingen, Anatomisches Institut, Abt. Zelluläre Neurobiologie, Germany. kroeger@anatu.uni-tuebingen.de
Wagner
H J
HJ
D000818:Animals; D002232:Carbocyanines; D002466:Cell Nucleolus / Q000648:ultrastructure; D002531:Cerebellum / Q000166:cytology*; D003600:Cytosol / Q000648:ultrastructure; D005456:Fluorescent Dyes; D007546:Isoquinolines; D018613:Microscopy, Confocal / Q000379:methods; D009474:Neurons / Q000166:cytology*; D010471:Perches; D051381:Rats; D012160:Retina / Q000166:cytology*
D002232:Carbocyanines; D005456:Fluorescent Dyes; D007546:Isoquinolines; C024286:3,3'-dioctadecylindocarbocyanine; C017475:lucifer yellow