title: Microcystin uptake inhibits growth and protein phosphatase activity in mustard (Sinapis alba L.) seedlings.
abstract: Mustard (Sinapis alba L.) seeds were cultivated for seven days on a solid nutrient medium supplemented with 040 microg microcystin-RR per ml. Microcystin-RR affected seedling growth (IC50 0.8 microg/ml) and microcystin concentrations > or =5.0 microg/ml produced malformed plants. The inhibition of protein phosphatase 1 and 2A activity correlated with the growth inhibition. The seedlings were also shown to take up 3H-dihydromicrocystin-LR derived radioactivity up to a level corresponding to ca. 80 ng toxin per mg plant protein.
authors.affiliation: Department of Biochemistry and Pharmacy, Abo Akademi University, Turku, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Kurki-Helasmo
authors.forename: J
authors.initials: J
authors.lastname: Meriluoto
mesh_terms: D001427:Bacterial Toxins / Q000493:pharmacokinetics / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury; D000458:Cyanobacteria / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D052998:Microcystins; D009149:Mustard Plant / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D010456:Peptides, Cyclic / Q000494:pharmacology*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*; D010946:Plants, Medicinal; D054645:Protein Phosphatase 1
chemical_list: D001427:Bacterial Toxins; D004791:Enzyme Inhibitors; D052998:Microcystins; D010456:Peptides, Cyclic; C078588:microcystin; D010749:Phosphoprotein Phosphatases; D054645:Protein Phosphatase 1