Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain.
A unique clone, isolated from a human pancreatic cDNA library, was sequenced and characterized. Northern blot analysis showed that the gene is active in a number of fetal and adult tissues, and immunoblots showed expression in nuclear and cytosolic cell fractions. The gene corresponding to the clone was localized to chromosome 13 by human/rodent somatic cell hybrid panels. The largest open reading frame contains a LIM domain, and the deduced peptide from the open reading frame appears to have the characteristics of a LIM-only protein, designated LMO7. RT-PCR and genomic sequence analyses indicate that expression of this gene product is subject to tissue-specific modulation by elimination of the LIM domain by alternative splicing in neural tissues.
Putilina
T
T
Laboratory of Retinal Cell and Molecular Biology, National Eye Institute, Bethesda, Maryland, USA.
Jaworski
C
C
Gentleman
S
S
McDonald
B
B
Kadiri
M
M
Wong
P
P
D000328:Adult; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016384:Consensus Sequence; D018076:DNA, Complementary / Q000235:genetics*; D005333:Fetus / Q000378:metabolism; D014644:Genetic Variation; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D060588:LIM Domain Proteins; D008969:Molecular Sequence Data; D009417:Nerve Tissue / Q000378:metabolism; D010179:Pancreas / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics*
D018076:DNA, Complementary; D018398:Homeodomain Proteins; D060588:LIM Domain Proteins; C116184:LMO7 protein, human; D014157:Transcription Factors