title: Analysis of 3p allelic losses in renal cell carcinomas: comparison with cytogenetic results.
abstract: We performed a study of loss of heterozygosity (LOH) at 3p by restriction fragment length polymorphism analysis in a series of 22 renal tumors. In 11 cases, molecular results could be compared with those of cytogenetic studies. The highest frequency of allelic losses at 3p was seen in clear cell non-papillary renal tumors, whereas none of the papillary renal cell carcinomas showed LOH at 3p. Allelic losses on 3p were found to be independent of tumor grade or stage or both. One case analyzed showed a discrepancy between cytogenetic and LOH studies. This tumor displayed rearrangements of chromosome 3 and no LOH at the c-RAF-1 (close to the Von Hippel Lindau gene) locus.
authors.affiliation: Institut de Biologia Fonamental, Universitat Autònoma de Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Bernués
authors.forename: C
authors.initials: C
authors.lastname: Casadevall
authors.forename: R
authors.initials: R
authors.lastname: Miró
authors.forename: M R
authors.initials: MR
authors.lastname: Caballín
authors.forename: A
authors.initials: A
authors.lastname: Gelabert
authors.forename: M J
authors.initials: MJ
authors.lastname: Ejarque
authors.forename: G
authors.initials: G
authors.lastname: Chéchile
authors.forename: J
authors.initials: J
authors.lastname: Egozcue
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D007680:Kidney Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D019908:Proto-Oncogene Proteins c-raf / Q000235:genetics
chemical_list: D009363:Neoplasm Proteins; D019908:Proto-Oncogene Proteins c-raf