Expression of a fusion protein of scFv-biotin mimetic peptide for immunoassay.
We constructed two fusion proteins of scFv linked to biotin mimetic sequence (BMS) via different linkers, and expressed them in the Pichia pastoris expression/secretion system. We found that both bi-functional scFv proteins exhibited their intrinsic binding activities to antigen CA125 determined in competitive radioimmunoassay experiments, but the fusion protein with a spacer between the scFv and BMS (scFv-spacer-BMS) showed higher binding activity of streptavidin than the one with c-Myc peptide as a linker.
Luo
D
D
Research and Development, AltaRex Corp., University of Alberta, Edmonton, Canada.
Geng
M
M
Schultes
B
B
Ma
J
J
Xu
D Z
DZ
Hamza
N
N
Qi
W
W
Noujaim
A A
AA
Madiyalakan
R
R
D018033:Antibodies, Bispecific / Q000276:immunology*; D001710:Biotin / Q000737:chemistry / Q000378:metabolism*; D018394:CA-125 Antigen / Q000276:immunology; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000378:metabolism; D007135:Immunoglobulin Variable Region / Q000276:immunology* / Q000378:metabolism; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D016271:Proto-Oncogene Proteins c-myc; D011863:Radioimmunoassay / Q000379:methods; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D019809:Streptavidin / Q000378:metabolism
D018033:Antibodies, Bispecific; D018394:CA-125 Antigen; D007128:Immunoglobulin Fragments; D007135:Immunoglobulin Variable Region; D016271:Proto-Oncogene Proteins c-myc; D011993:Recombinant Fusion Proteins; D001710:Biotin; D019809:Streptavidin