title: MHC class I molecules compete in the endoplasmic reticulum for access to transporter associated with antigen processing.
abstract: We have used the functionally distinct TAP alleles of the rat in cellular transfectants as tools to investigate how newly formed rat class I (RT1.A) molecules with distinct peptide requirements gain access to suitable peptides in the endoplasmic reticulum (ER). Normal maturation of RT1.Aa depends on the presence in the ER of peptides with C-terminal arginine, while restrictive TAP-B allelic group transporters fail to transport such peptides. In this situation, RT1.Aa is retained in the ER. We show that this retention is accompanied by accumulation of RT1.Aa in the ER, partly associated with TAP and partly free. In such cells, access to TAP of a second allelic product, RT1.Au, which does not require C-terminal arginine peptides, is competitively inhibited by the build-up of RT1.Aa. Nevertheless, RT1.Au loads and matures normally. Introduction of a permissive TAP-A allele competent to transport C-terminal arginine peptides releases RT1.Aa from the ER and restores RT1.Au interaction with TAP. Both class I alleles associate indiscriminately with permissive and restrictive TAP alleles. The data support the view that interaction with TAP is not a prerequisite for peptide loading by class I molecules, so long as suitable peptides are available in the ER. They further show that TAP association of a class I molecule depends on a competitive balance in the ER defined by the extent to which the peptide requirements of other class I molecules present are satisfied and not only by the intrinsic strength of the interaction with TAP.
authors.affiliation: Institute for Genetics, University of Cologne, Germany. knittler@uni-koeln.de
authors.forename: M R
authors.initials: MR
authors.lastname: Knittler
authors.forename: K
authors.initials: K
authors.lastname: Gülow
authors.forename: A
authors.initials: A
authors.lastname: Seelig
authors.forename: J C
authors.initials: JC
authors.lastname: Howard
mesh_terms: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters / Q000276:immunology* / Q000378:metabolism*; D000818:Animals; D017951:Antigen Presentation; D017920:Antiporters; D001667:Binding, Competitive / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000378:metabolism; D037282:Calreticulin; D002352:Carrier Proteins / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000276:immunology* / Q000378:metabolism*; D006649:Histocompatibility Antigens / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D007136:Immunoglobulins; D007424:Intracellular Fluid / Q000276:immunology / Q000378:metabolism; D007700:Kinetics; D026901:Membrane Transport Proteins; D011485:Protein Binding / Q000276:immunology; D051381:Rats; D012261:Ribonucleoproteins / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D000071450:ATP Binding Cassette Transporter, Subfamily B, Member 3; D018528:ATP-Binding Cassette Transporters; D017920:Antiporters; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D002352:Carrier Proteins; D006649:Histocompatibility Antigens; D015395:Histocompatibility Antigens Class I; D007136:Immunoglobulins; D026901:Membrane Transport Proteins; D012261:Ribonucleoproteins; C489514:Tap2 protein, rat; C038353:histocompatibility antigens RT, rat; C107762:tapasin