Dendrite classification in rat hippocampal neurons according to signal propagation properties. Observation by multichannel optical recording in cultured neuronal networks.
Two-dimensional neuronal networks were formed using a dissociated culture of rat hippocampal neurons on glass plates. Neural activity in response to pulse stimuli applied to the neurons by whole-cell clamp electrodes was observed with a 128-channel optical recording apparatus using a voltage-sensitive dye, RH482. Dendrites emerging from the somata of single neurons were classified according to two signal-transmission properties, those with lower conduction velocities (0.12+/-0.034 m/s, n=24) and those with very fast conduction velocity (faster than 1.0 m/s), by evaluating the conduction velocities of pulse responses. The distinction between these two properties seemed to be related to the morphological differences in input connectivity with the axons of neighboring neurons.
Kawaguchi
H
H
Advanced Research Laboratory, Hitachi Ltd., Hatoyama, Saitama, Japan.
Fukunishi
K
K
D000200:Action Potentials / Q000502:physiology; D000818:Animals; D002478:Cells, Cultured; D003712:Dendrites / Q000145:classification*; D004558:Electric Stimulation; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D007150:Immunohistochemistry; D007834:Lasers; D009415:Nerve Net / Q000502:physiology*; D009431:Neural Conduction; D009474:Neurons / Q000502:physiology*; D055095:Optics and Photonics; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D011930:Reaction Time / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*