title: The effect of H. pylori in perforation of duodenal ulcer.
abstract: OBJECTIVE
  H. pylori has been described as an opportunistic pathogen attracted by changes in the gastric mucosa caused by inflammation and ulceration. However, the role of H. pylori infection in the perforation of duodenal ulcers has not yet been clearly determined. The aim of this study was to assess the prevalence of H. pylori infection in patients undergoing laparotomy for repair of a perforated duodenal ulcer.
  
  
  METHODS
  Patients who underwent surgery for a perforated duodenal ulcer in our Surgical Unit between January 1994 and July 1996 were included in this study. The study population consisted of eighteen patients with a mean age of 32.7 (21-48) years. All of the patients were male. Patients with chronic duodenal ulcer perforation and with no contraindications for definitive surgery, such as peritonitis, shock (blood pressure <90 mm Hg), age >60 years, or more than a 12-hour elapse from the time of perforation, were treated by bilateral truncal vagotomy and Weinberg pyloroplasty. The ulcer was excised with the pyloric ring. The cut was then extented by about 2 cm on both the gastric and duodenal sides. Two biopsies were taken from the antral mucosa by endoscopic biopsy forceps. The defect was closed transversely. The ulcer specimen and the antral biopsies were fixed separately in 10% formalin solution and sent to the department of Histopathology. The specimens were stained with Hematoxylin-Eosin and examined for H. pylori . Sections of the ulcer specimen were especially investigated for the presence of H. pylori through all layers of the ulcer.
  
  
  RESULTS
  H. pylori was found in the antral biopsies of 16 patients (88.8%). In seven of the ulcer specimens (38.8%), H. pylori was present in the mucosa and also extended through the wall of the ulcer. H. pylori was positive in the antral biopsies of all patients with H. pylori present in the ulcer wall.
  
  
  CONCLUSIONS
  In our study, H. pylori was present at a high ratio in the antral biopsies of patients with duodenal ulcer perforation. The presence of H. pylori throughout the ulcer wall to a considerable extent emphasizes the fact that eradication of H. pylori is important in the treatment of perforated duodenal ulcer.
authors.affiliation: Department of Surgery and Pathology, Sisli Etfal Training Hospital, Istanbul, Turkey.
authors.forename: M
authors.initials: M
authors.lastname: Mihmanli
authors.forename: A
authors.initials: A
authors.lastname: Isgor
authors.forename: F
authors.initials: F
authors.lastname: Kabukcuoglu
authors.forename: B
authors.initials: B
authors.lastname: Turkay
authors.forename: B
authors.initials: B
authors.lastname: Cikla
authors.forename: A
authors.initials: A
authors.lastname: Baykan
mesh_terms: D000328:Adult; D004381:Duodenal Ulcer / Q000150:complications / Q000382:microbiology* / Q000601:surgery; D005753:Gastric Mucosa / Q000382:microbiology; D016481:Helicobacter Infections / Q000150:complications*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D010439:Peptic Ulcer Perforation / Q000382:microbiology* / Q000601:surgery