Stress-induced tau phosphorylation in mouse strains with different brain Erk 1 + 2 immunoreactivity.
Quantitative blot immunolabeling techniques were used to compare the effect of cold water stress (CWS) on the phosphorylation of brain tau protein in two strains of C57BL/6J mice: ob/ob and ob/+. CWS induced immediate, significant (4-6 fold) and continuous accumulation of Alzheimer's like forms of tau phosphorylated at Ser 202. The effect was considerably (150-200%) higher in ob/ob mice compared to ob/+ mice. By contrast, the unphosphorylated tau immunoreactivity decreased after the CWS, whereas no changes were detected in the abundance of several other antigens. The level of anti Erk 1 + 2 immunoreactivity corresponding to 36-37 kDa polypeptide was 2-3 fold higher in ob/ob than in ob/+ mice. No strain differences were detected in the abundance of several other protein kinases. Obtained results support the hypothesis that Erk 1 or 2 like protein kinase may be involved in the restructuring of neuronal cytoskeleton in response to environmental stress.
Korneyev
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AY
Floraleads GR, Lake Forest, IL 60045, USA.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D005260:Female; D051379:Mice; D008820:Mice, Obese; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D010766:Phosphorylation; D013312:Stress, Physiological / Q000378:metabolism*; D016875:tau Proteins / Q000378:metabolism*
D016875:tau Proteins; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases