Gas chromatography with electron-capture and nitrogen-phosphorus detection in the analysis of pesticides in honey after elution from a Florisil column. Influence of the honey matrix on the quantitative results.
A modified procedure to extract pesticides from honey samples that involves loading the honey onto a Florisil packed column and subsequently eluting it with an n-hexane-dichloromethane mixture is proposed. Anomalous high gas chromatographic responses and subsequently very high recoveries for the pesticides in the extracts were obtained by a conventional calibration with pesticide solutions in organic solvent. This effect was attributed to the honey matrix and can be circumvented by using spiked honey extracts as calibration standards.
Jiménez
J J
JJ
Department of Analytical Chemistry, Faculty of Sciences, University of Valladolid, Spain.
Bernal
J L
JL
del Nozal
M J
MJ
Toribio
L
L
Martín
M T
MT
D002849:Chromatography, Gas / Q000379:methods*; D006586:Hexanes; D006722:Honey / Q000032:analysis*; D017633:Magnesium Silicates; D008752:Methylene Chloride; D010575:Pesticides / Q000032:analysis*
D006586:Hexanes; D017633:Magnesium Silicates; D010575:Pesticides; C005013:Florisil; C026385:n-hexane; D008752:Methylene Chloride