title: Fibroblasts from Alzheimer's disease donors do not differ from controls in response to heat shock.
abstract: We investigated HSPs mRNA expression in cultured fibroblasts from control and AD patients. Northern blot analysis using probes for HSP70 and HSC73 revealed that HS induced a several fold increase in both mRNA. Under these condition the extent of mRNA increase was similar in controls and AD. HS elicited also a modest increase in sAPP release that was similar in control and AD. The results suggest that the ability of AD fibroblasts to produce a defensive response to HS is preserved.
authors.affiliation: Department of Internal Medicine, University of Milan and Ospedale Maggiore, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Arosio
authors.forename: G
authors.initials: G
authors.lastname: Annoni
authors.forename: C
authors.initials: C
authors.lastname: Vergani
authors.forename: D C
authors.initials: DC
authors.lastname: Solano
authors.forename: M
authors.initials: M
authors.lastname: Racchi
authors.forename: S
authors.initials: S
authors.lastname: Govoni
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000473:pathology / Q000503:physiopathology*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000502:physiology*; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006358:Hot Temperature; D006801:Humans; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D012016:Reference Values; D012769:Shock / Q000473:pathology / Q000503:physiopathology*; D012995:Solubility
chemical_list: D016564:Amyloid beta-Protein Precursor; D002352:Carrier Proteins; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C494371:HSPA8 protein, human; D012333:RNA, Messenger