Growth and oil production of Apiotrichum curvatum in tomato juice.
The lipid-accumulating yeast Apiotrichum curvatum ATCC 20509 (formerly Candida curvata D) grew in shake flask culture in freshly prepared tomato juice or tomato pulp; growth was improved when the medium was supplemented with sucrose and a basal salts mixture. Under controlled conditions in the fermentor, biomass and oil production reached a maximum at 96 h, compared with 130 h in shake flasks. Biomass production increased with the addition of 0.3% (wt/vol) urea to the juice, but decreased with the addition of 0.5% (wt/vol) yeast extract or Casamino Acids. The total amount of lipid produced and the percent of intracellular oil per cell dry weight were greatest in juice supplemented with 0.3% urea.
Akindumila
F
F
Department of Food Science and Human Nutrition, Iowa State University, Ames 50011, USA.
Glatz
B A
BA
D018533:Biomass; D002175:Candida / Q000254:growth & development* / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation; D007785:Lactose / Q000378:metabolism; D018551:Solanum lycopersicum; D009821:Oils / Q000378:metabolism*
D003470:Culture Media; D009821:Oils; D007785:Lactose