title: Interleukin 1alpha stimulates lactate dehydrogenase A expression and lactate production in cultured porcine sertoli cells.
abstract: By using cultured porcine Sertoli cells as a model, the action of interleukin 1alpha (IL-1alpha) on lactate production and the site of this action were studied. IL-1alpha stimulated Sertoli cell lactate production in a time- and dose-dependent manner (with a half-maximal effect [ED50] of 6 pM). Two major sites involved in IL-1alpha action were identified. First, IL-1alpha was shown to increase the uptake of glucose substrate in a time- and dose-dependent manner. The maximal effect, with an ED50 of 10 pM, was observed after 24 h of treatment. Second, IL-1alpha increased the activity of the lactate dehydrogenase (LDH) A4 isoform, which is involved in the conversion of pyruvate into lactate. This increase in LDH A4 activity was detected at 12 h and was maximal, with an ED50 of 9 pM, after 24-h treatment with IL-1alpha. The increase was related to an increase in LDH A4 expression, since IL-1alpha stimulated LDH A mRNA (size: 1.5 kilobases, evidenced through Northern blotting analysis) in a dose- and time-dependent manner. Assuming that IL-1alpha might be produced in the seminiferous tubules by both Sertoli and germ cells, which utilize lactate for their energy metabolism, we suggest that these results together show 1) that the cytokine may represent a signal in the metabolic cooperation existing between Sertoli cells and germ cells, and 2) that a redistribution of LDH isoforms in favor of LDH A4 under IL-1alpha control is a key mechanism(s) in such cooperation used by germ cells to enhance lactate production in Sertoli cells.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U407, Communications Cellulaires en Biologie de la Reproduction, Centre Hospitalier Lyon-Sud, 69 495 Pierre-Bénite, France.
authors.forename: D
authors.initials: D
authors.lastname: Nehar
authors.forename: C
authors.initials: C
authors.lastname: Mauduit
authors.forename: F
authors.initials: F
authors.lastname: Boussouar
authors.forename: M
authors.initials: M
authors.lastname: Benahmed
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D015870:Gene Expression; D005947:Glucose / Q000378:metabolism; D007375:Interleukin-1 / Q000494:pharmacology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D019344:Lactic Acid / Q000096:biosynthesis*; D008297:Male; D019289:Pyruvic Acid / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012708:Sertoli Cells / Q000201:enzymology*; D013552:Swine
chemical_list: D007375:Interleukin-1; D007527:Isoenzymes; D012333:RNA, Messenger; D019344:Lactic Acid; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase; D005947:Glucose