A Pseudomonas aeruginosa biosensor responds to exposure to ultraviolet radiation.
We fused the Pseudomonas aeruginosa recA promoter to a promoterless Vibrio fisheri lux operon. This recA-lux fusion (pMOE15) was introduced into wild-type P. aeruginosa strain FRD1 and recA expression was monitored by measuring 490-nm light production. The RM4440 strain responded to increasing doses of ultraviolet radiation by an increase in its bioluminescence. RM4440 has the potential to be useful as a biosensor for the presence of DNA-damaging agents in the environment.
Elasri
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MO
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078, USA.
Miller
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RV
D015374:Biosensing Techniques / Q000379:methods*; D004249:DNA Damage; D008163:Luminescent Measurements; D009876:Operon; D011401:Promoter Regions, Genetic; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000528:radiation effects*; D011938:Rec A Recombinases / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014169:Transformation, Bacterial; D014466:Ultraviolet Rays; D014733:Vibrio / Q000235:genetics
D011938:Rec A Recombinases