Influence of somatostatin on acute pancreatitis in rats.
OBJECTIVE
To assess the effect of two regimens of somatostatin on the morbidity and short term outcome of acute pancreatitis in rats.


METHODS
Randomised laboratory study.


METHODS
Teaching hospital, France.


METHODS
24 male Wistar rats.


METHODS
Rats were randomised to 3 groups of 8, 2 of which were given somatostatin (one 25 microg/kg/hour by continuous infusion and one as a bolus injection of 5 microg before the infusion of 25 microg/kg/hour); the third (control) group was given 0.9% of saline 25 microg/kg/hour. Acute pancreatitis was induced by ligating the pancreatic duct at the junction with the duodenum.


METHODS
Serum amylase and lipase activities at 2 and 4 hours, and histological changes after 4 hours, at which time the animals were killed and the pancreas removed.


RESULTS
Amylase and lipase activities were significantly lower in the somatostatin groups than in the control group throughout the experiment (p < 0.001). They were slightly but not significantly lower in the bolus group than the infusion group. The degree of necrosis and the extent of inflammatory infiltration were significantly less in the somatostatin groups (p < 0.008).


CONCLUSIONS
Somatostatin has an early favourable effect on the course of experimentally induced pancreatitis in rats.
Berthet
B
B
Department of Surgery, Hôpital de la Timone, Marseille, France.
Guillou
N
N
Brioche
M I
MI
Choux
R
R
Viret
P
P
Ledoray
V
V
Billardon
M
M
Assadourian
R
R
D000208:Acute Disease; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000473:pathology; D010195:Pancreatitis / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000008:administration & dosage*; D013997:Time Factors
D013004:Somatostatin