title: The effect of limited exposure to antimycotics on the relative cell-surface hydrophobicity and the adhesion of oral Candida albicans to buccal epithelial cells.
abstract: Candida albicans is the major aetiological agent of oral candidosis. Adhesion to oral mucosal surfaces is considered a prerequisite for its successful colonization and subsequent infection, and its relative cell-surface hydrophobicity (CSH) is a contributory physical force. Thus, the main aim here was to determine the CSH of 10 isolates of oral C. albicans after a short exposure to sublethal concentrations of four antifungal agents and to correlate these findings with their adhesion to human buccal epithelial cells (BEC). The yeasts were exposed to sublethal concentrations of nystatin [x 6 minimal inhibitory concentration (MIC)], 5-fluorocytosine (x 8 MIC), ketoconazole (x 4 MIC) and fluconazole (x 4 MIC) for 1 h. The drug was then removed, and the CSH and BEC adhesion assessed by a biphasic aqueous-hydrocarbon assay and a microscopic method, respectively. The mean percentage reductions of CSH after exposure to nystatin, 5-fluorocytosine, ketoconazole and fluconazole were 27.14% (p = 0.01), 9.46% (p = 0.43), 19.47% (p = 0.04) and 6.16% (p = 0.59). Similarly, exposure to all the drugs except 5-fluorocytosine resulted in a significant inhibition of yeast adhesion to BEC, with nystatin eliciting the highest and fluconazole the least inhibition. Further, on regression analysis a strong positive correlation was observed between CSH and adhesion to BEC after limited exposure to 5-fluorocytosine (r = 0.48, p < 0.0001), ketoconazole (r = 0.48, p < 0.0001), fluconazole (r = 0.55, p < 0.0001) as well as in the unexposed controls (r = 0.41, p = 0.001), although nystatin was an exception (r = 0.09, p = 0.44). Taken together, these data elucidate further mechanisms by which antimycotics may operate in vivo to suppress candidal pathogenicity.
authors.affiliation: Faculty of Dentistry, Prince Philip Dental Hospital, University of Hong Kong.
authors.forename: A N
authors.initials: AN
authors.lastname: Ellepola
authors.forename: L P
authors.initials: LP
authors.lastname: Samaranayake
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D002610:Cheek; D003470:Culture Media; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology*; D005260:Female; D006679:HIV Seropositivity / Q000382:microbiology; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D016014:Linear Models; D008297:Male; D009055:Mouth / Q000382:microbiology*; D013499:Surface Properties
chemical_list: D000935:Antifungal Agents; D003470:Culture Media