A simple and rapid method for preparing genomic DNA from gram-positive bacteria.
Molecular epidemiologic and other studies may require preparation of genomic DNA from large numbers of bacteria in sufficiently pure form for restriction endonuclease digestion, cloning, RAPD-PCR, Southern hybridization, and so on. Staphylococcus and other Gram-positive bacteria have a rigid cell wall and can be difficult to lyse. Here, a simple and rapid method for the preparation of genomic DNA from multiple samples is reported. This method produces clean DNA for use in most molecular biology methods in < 90 min.
Sohail
M
M
Biochemistry Department, University of Oxford, England, UK. msohail@bioch.ox.ac.uk
D001407:Bacillus / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D016680:Genome, Bacterial; D006094:Gram-Positive Bacteria / Q000235:genetics*; D015203:Reproducibility of Results; D013210:Staphylococcus / Q000235:genetics
D004269:DNA, Bacterial