Effects of glucose on chemically induced acute hepatic failure in rats.
OBJECTIVE
Acute hepatic failure (AHF) is a physiopathological entity difficult to reproduce experimentally but necessary to evaluate new therapies such as bioartificial liver (BAL) or hepatocyte transplantation (HcTX). Two main animal models are available: surgical or chemical induction of an AHF. Many authors have assessed BAL or HcTX with such models and observed increased survival rate in the treated groups. However, during AHF, severe hypoglycemia takes place. Some authors have shown that adjunction of glucose in beverage after surgically induced AHF may induce by itself an improvement of survival rate.


METHODS
The purpose of the present study is to test if the adjunction of glucose leads to the same effect in an AHF chemically induced by D-Galactosamine (D-Gal). A group of animals (rats) receiving glucose 20% as beverage immediately after intoxication (Group 1), is compared with a control group receiving tap water (Group 2).


RESULTS
The required dose to achieve AHF is 2.75 g/kg body weight (Gr. 0). Survival rates are as follows: Gr. 1: Day 3:20%; day 5: 10%; day 8: 0%. Gr. 2: Day 3: 0%. The difference in survival rate is not statistically significant between the two groups. Liver enzymes peak at 24 hours, then return to normal values; blood sugar levels show no difference between groups. Histological examination of Gr. 0 animals surviving and sacrificed on day 28 shows restitutio ad integrum of hepatic structure.


CONCLUSIONS
Glucose adjunction does not alter survival rate. We can conclude that this particular model of AHF is reliable.
Birraux
J
J
Department of Paediatrics, Children's Hospital and Hôpital Cantonal, University Hospitals of Geneva, Switzerland.
Genin
B
B
Sinigaglia
C
C
Mage
R
R
Morel
P
P
Le Coultre
C
C
D000818:Animals; D004195:Disease Models, Animal; D005947:Glucose / Q000627:therapeutic use*; D017114:Liver Failure, Acute / Q000188:drug therapy* / Q000503:physiopathology; D008111:Liver Function Tests; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results
D005947:Glucose