Sexing of human and other primate DNA.
We present a rapid and simple method for the sexing of human and other primate DNA. Homologous introns from the X- and Y-linked zinc finger protein genes (ZFX and ZFY) are PCR amplified with one primer pair. An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by agarose gel electrophoresis.
Wilson
J F
JF
Zoologisches Institut, Universität München, Germany.
Erlandsson
R
R
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000235:genetics; D008040:Genetic Linkage; D005826:Genetics, Medical; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D011323:Primates / Q000235:genetics*; D019849:Sex Determination Processes; D014157:Transcription Factors; D014960:X Chromosome; D014998:Y Chromosome; D016335:Zinc Fingers
D017931:DNA Primers; D004268:DNA-Binding Proteins; D051741:Kruppel-Like Transcription Factors; D014157:Transcription Factors; C054430:ZFY protein, human; C062076:zinc finger protein, X-linked