Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1-->3),(1-->4)-Beta-D-glucan 4-glucanohydrolase.
Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield.
Viladot
J L
JL
Laboratori de Bioquímica, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Stone
B
B
Driguez
H
H
Planas
A
A
D001407:Bacillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry*; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*
D009844:Oligosaccharides; D002480:Cellulase