Tissue distribution of the guinea-pig decay-accelerating factor.
MCA44 is a monoclonal antibody (mAb) to guinea-pig decay-accelerating factor (DAF) and, using this mAb, tissue distribution of guinea-pig DAF was studied by immunofluorescence. Guinea-pig DAF was found to be expressed not only on the vascular endothelium but also on different types of cells, such as the tubular epithelium of the kidney, epidermal cells of the skin and synovial lining cells. As there was no significant reduction in staining intensity with MCA44 following treatment with phosphatidylinositol-specific phospholipase C, many guinea-pig DAF molecules expressed in these tissues may be of the transmembrane form.
Nishikawa
K
K
Third Department of Internal Medicine, Nagoya University School of Medicine, Nagoya, Japan.
Matsuo
S
S
Tamai
H
H
Okada
N
N
Okada
H
H
D000818:Animals; D001921:Brain / Q000276:immunology; D018958:CD55 Antigens / Q000378:metabolism*; D002196:Capillaries / Q000276:immunology; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D009206:Myocardium / Q000276:immunology; D010053:Ovary / Q000276:immunology; D012867:Skin / Q000276:immunology; D013583:Synovial Membrane / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution; D001743:Urinary Bladder / Q000276:immunology; D014599:Uterus / Q000276:immunology
D018958:CD55 Antigens