Role of IL-1 beta and TNF-alpha in the regulation of NGF in experimentally induced arthritis in mice.
The aim of this study was to investigate the effects of IL-1 beta and TNF-alpha on NGF levels in the knee joint in experimentally induced arthritis in adult mice. Out data showed that IL-1 beta, but not TNF-alpha, induces an increase in NGF levels, while concomitant injection of both cytokines enhances the effect of IL-1 beta on NGF presence. Analysis of NGF levels in the knee joint of carrageenan- and IL-1 beta-induced inflammation after administration of antibodies against TNF-alpha supports this hypothesis. Our studies also showed that exogenous administration of NGF antibody reduces the enhanced level of TNF-alpha occurring in arthritic mice. This latter observation indirectly suggests that NGF is implicated in the upregulation of TNF-alpha in these animal models of joint inflammation. The functional significance of NGF or NGF antibody in inflammatory arthritis is discussed.
Manni
L
L
Institute of Neurobiology (CNR), Rome, Italy.
Aloe
L
L
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000378:metabolism*; D002351:Carrageenan / Q000633:toxicity; D007375:Interleukin-1 / Q000494:pharmacology* / Q000502:physiology; D007719:Knee Joint / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009414:Nerve Growth Factors / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology* / Q000502:physiology; D015854:Up-Regulation
D000911:Antibodies, Monoclonal; D007375:Interleukin-1; D009414:Nerve Growth Factors; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D002351:Carrageenan