title: Simian-virus-40 footprints in human lymphoproliferative disorders of HIV- and HIV+ patients.
abstract: SV40 sequences were investigated by PCR DNA amplification followed by filter hybridization in a series of human lymphoproliferative disorders obtained from human-immunodeficiency-virus (HIV)-seronegative and HIV-infected patients. Our PCR and filter-hybridization conditions enabled us to detect SV40 sequences in the range of 10(-4) to 10(-2) genome equivalents per cell. In non-Hodgkin's lymphomas (NHL) from HIV- patients, SV40 footprints were found in 11 out of 79 (13.9%) samples, while in NHL from HIV+ patients SV40 DNA sequences were detected in 2/16 (12.5%). In Hodgkin's disease (HD), SV40 sequences were found in 7/43 (16.3%) and 1/12 (8.3%) in HIV- and HIV+ patients respectively. A slightly higher prevalence of SV40 footprints was observed in reactive lympho-adenopathies both in HIV- (3/9, 33.3%) and in HIV+ (6/17, 35.3%) patients. Sequence analysis of 2 NHL and 2 HD DNA samples established that the amplified PCR products belong to the SV40 sequences. SV40 prevalence and load were similar in samples from HIV-seronegative and HIV-infected individuals, suggesting that SV40 probably does not undergo strong reactivation phenomena in the context of HIV-related immunosuppression. Moreover, the large T-antigen(Tag) expression was detected by immunohistochemistry in 5/18 SV40-DNA-positive samples analyzed; however, few tumor cells (<1%) in 3/5 samples displayed positivity for SV40 Tag, while this viral oncoprotein was revealed in several reactive histiocytes present in all 5 SV40-positive tissues. These results suggest that the lymphoid tissue could represent a reservoir for SV40 and may constitute the first step in understanding whether this DNA tumor polyomavirus has a role in the pathogenesis of human lymphoproliferative disorders.
authors.affiliation: Department of Morphology and Embryology, University of Ferrara, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Martini
authors.forename: R
authors.initials: R
authors.lastname: Dolcetti
authors.forename: A
authors.initials: A
authors.lastname: Gloghini
authors.forename: L
authors.initials: L
authors.lastname: Iaccheri
authors.forename: A
authors.initials: A
authors.lastname: Carbone
authors.forename: M
authors.initials: M
authors.lastname: Boiocchi
authors.forename: M
authors.initials: M
authors.lastname: Tognon
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D006689:Hodgkin Disease / Q000821:virology; D006801:Humans; D016483:Lymphoma, AIDS-Related / Q000821:virology; D008228:Lymphoma, Non-Hodgkin / Q000821:virology; D008232:Lymphoproliferative Disorders / Q000821:virology*; D016133:Polymerase Chain Reaction; D013539:Simian virus 40 / Q000235:genetics*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D004279:DNA, Viral