title: Construction of a bioluminescent reporter strain To detect polychlorinated biphenyls
abstract: A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.
authors.affiliation: The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, Tennessee 37996-1605, USA.
authors.initials: AC
authors.lastname: Layton
authors.initials: M
authors.lastname: Muccini
authors.initials: MM
authors.lastname: Ghosh
authors.initials: GS
authors.lastname: Sayler