Enteropathogenic Escherichia coli virulence genes encoding secreted signalling proteins are essential for modulation of Caco-2 cell electrolyte transport.
The pathophysiology of enteropathogenic Escherichia coli (EPEC) diarrhea remains uncertain. In vitro, EPEC stimulates a rapid increase in short-circuit current (Isc) across Caco-2 cell monolayers coincident with intimate attaching and effacing (A/E) bacterial adhesion. This study has examined the roles of specific EPEC virulence proteins in this Isc response. EPEC genes encoding EspA, EspB, and EspD, essential for signal transduction in host cells and A/E activity, were also required for modulation of Caco-2 electrolyte transport.
Collington
G K
GK
Institute of Child Health, University of Birmingham, Birmingham B4 6NH, United Kingdom. g.k.collington@bham.ac.uk
Booth
I W
IW
Donnenberg
M S
MS
Kaper
J B
JB
Knutton
S
S
D018829:Adhesins, Bacterial; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000096:biosynthesis / Q000378:metabolism; D001426:Bacterial Proteins / Q000378:metabolism*; D001692:Biological Transport; D018938:Caco-2 Cells; D002352:Carrier Proteins; D003106:Colon / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D004553:Electric Conductivity; D017097:Electric Impedance; D004573:Electrolytes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins; D005798:Genes, Bacterial; D006801:Humans; D015398:Signal Transduction
D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; C087863:EaeB protein, E coli; D004573:Electrolytes; D029968:Escherichia coli Proteins; C102051:EspA protein, E coli; C073819:eaeA protein, E coli