title: Influence of magnesium substitution on a collagen-apatite biomaterial on the production of a calcifying matrix by human osteoblasts.
abstract: The induction of a calcifying matrix is of great interest in the restoration of bone defects. In a previous in vitro study we demonstrated that a collagen sponge constituted of type I collagen fibrils, chondroitin sulfates, and hydroxyapatite crystals induces an earlier and a more abundant synthesis of a new extracellular calcifying matrix than do other biomaterials such as collagen or hydroxyapatite alone. Bone mineral contains various amounts of magnesium ions, either adsorbed at the surface of apatite crystals or incorporated inside the crystal structure. Magnesium is known to reduce the degradation rate of tricalcium phosphate ceramics and to influence the crystallization of mineral substance. Thus we evaluated two sponges modified with different substituted apatites. The substituted low magnesium-containing apatite sample decreased the osteoinductive properties of the sponge whereas the substituted high magnesium-containing apatite sample had a toxic effect on bone cells and prevented the formation of any extracellular matrix. Such a toxic effect can be explained by the presence of large numbers of magnesium ions released into the culture medium even though at physiological level magnesium is able to promote bone mineralization and to control the growth of hydroxyapatite crystals. Thus collagen sponges containing hydroxyapatite remain one of the most appropriately evaluated biomaterials used for the restoration of periodontal pockets and bone defects.
authors.affiliation: INSERM Unité 403, Faculté de Médecine RTH Laënnec, Lyon, France.
authors.forename: C M
authors.initials: CM
authors.lastname: Serre
authors.forename: M
authors.initials: M
authors.lastname: Papillard
authors.forename: P
authors.initials: P
authors.lastname: Chavassieux
authors.forename: J C
authors.initials: JC
authors.lastname: Voegel
authors.forename: G
authors.initials: G
authors.lastname: Boivin
mesh_terms: D001031:Apatites; D018786:Bone Substitutes; D002113:Calcification, Physiologic; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D006801:Humans; D008274:Magnesium / Q000494:pharmacology*; D009336:Necrosis; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D010012:Osteogenesis; D010587:Phagocytosis; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D001031:Apatites; D018786:Bone Substitutes; D008274:Magnesium