Kinetics of T cell proliferation: a mathematical model and data analysis.
A mathematical model of in vitro T cell proliferation controlled by IL-2 internalization is presented. The model describes the T cell transition from G1 to S+G2+M stages of the cell cycle and introduces "molecular" equations for the G1-S phase control. These equations consider current knowledge of the biochemical mechanisms of receptor synthesis, ligand-receptor binding, and internalization of ligand-receptor complexes. The model describes the kinetic data for in vitro T cell proliferation at various IL-2 concentrations (50-500 pM) for various exposure time (6-26 h). The kinetic parameters were calculated based on the model. The results obtained suggest that increase in the IL-2 concentration and exposures decrease the critical ligand-receptor concentration in the cells which control proliferation. The time tau, which characterizes the delay in the G1-S transition, was constant at various IL-2 concentrations.
Borisova
L R
LR
Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia. bioimath@sky.chph.ras.ru
Andreev
S G
SG
Kuznetsov
V A
VA
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016193:G1 Phase / Q000502:physiology*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology / Q000502:physiology*; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects; D018448:Models, Immunological; D015375:Receptors, Interleukin-2 / Q000187:drug effects / Q000502:physiology; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*
D007376:Interleukin-2; D015375:Receptors, Interleukin-2