The effect of alpha-tocopherol and pentoxyfilline on ischemia-reperfusion induced liver injury in rats.
OBJECTIVE
In the present study our purpose was to investigate the effect of pentoxyfilline, that plays a role in microcirculation and tissue oxygenation, alone and in combination with an antioxidant vitamin E on tissue damage in the rat liver induced by ischemia-reperfusion.


METHODS
Thirty-one albino rats were divided into four groups. Rats in group I (n= 7), group II (n= 8) and group III (n= 8) were given, respectively, pentoxyfilline (25 mg/kg), pentoxyfilline and vitamin E in combination (25 mg/kg and 50 mg/kg, respectively) and equal volume of saline solution intraperitoneally for 7 days. Rats in group IV (n= 8) served as controls and received no treatment. On day 7 ischemia was induced by cross-clamping the hepatic artery, portal vein and left branch of the biliary duct for 30 minutes. Malondialdehyde (MDA) and catalase activity were assessed in tissue sample, and the level of ALT was measured in serum obtained after reperfusion for 30 minutes. Histological examination of tissue sample was also carried out.


RESULTS
There was no significant difference in ALT level between three study groups. Group I and group II had significant lower MDA and catalase levels than those of group III. The results of histopathologic examination in group I and group II were better than that of group III.


CONCLUSIONS
Our findings suggested that the treatment of pentoxyfilline alone and in combination with vitamin E decreased liver damage induced by ischemia-reperfusion and that the effect of latter was more effective but the difference between the two treatment patterns was not statistically significant.
Vardareli
E
E
Osmangazi University Medical School, Department of Gastroenterology, Eskisehir, Turkey.
Saricam
T
T
Koken
T
T
Degirmenci
I
I
Aral
E
E
Erenoglu
E
E
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D010431:Pentoxifylline / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D014665:Vasodilator Agents / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D014665:Vasodilator Agents; D014810:Vitamin E; D008315:Malondialdehyde; D002374:Catalase; D000410:Alanine Transaminase; D010431:Pentoxifylline