title: In vivo effect on pig chromosomes of high dosage vaccine against classic swine fever.
abstract: Hog cholera virus (HCV) can induce chromosome abnormalities in diseased pigs as well as in those vaccinated with attenuated virus vaccine against classic swine fever. An experiment was made using animals from potency and safety control tests of commercial vaccines in Argentina. The different types of chromosomal alterations observed were chromatid and chromosome breaks, chromatid exchanges, polyploid, multiple aberrations cells, and chromosome pulverization. In this study the occurrence of chromosome alterations in pigs receiving either 1 or 10 vaccine doses was evaluated by means of blood sampling at different periods after vaccination. An essay comparing prolificity between treated and non-treated sows was also made. Significant differences in the amount of damaged chromosomes as well as differences in the type of predominant alterations between the two treatments were observed. Aberration frequencies increased from the 5-day postvaccination period reaching the highest value of 4.14% at the 10th, for the one-dose treatment; and highest value of 42.7% including 33.96% of cell with chromosome pulverization which was found in the 7th day interval when applying 10 doses. From then on, the proportion of affected cells dropped until the 20th day interval, which was the last recorded. The prolificity trial did not show any difference between treated and control sows, indicating that chromosome alteration might be limited to lymphocytes. It is concluded that HCV maintains its mutagenic potentiality in the attenuated vaccine, being able to induce chromosomal damage as it does in classic swine fever diseased animals.
authors.affiliation: Genética, Departamento de Producción Animal, Universidad Nacional de Río Cuarto, 5800, Río Cuarto, Argentina.rgenghini@ayv.unrc.edu.ar
authors.forename: R
authors.initials: R
authors.lastname: Genghini
authors.forename: I
authors.initials: I
authors.lastname: Tiranti
authors.forename: G
authors.initials: G
authors.lastname: Segade
authors.forename: J
authors.initials: J
authors.lastname: Amado
authors.forename: P
authors.initials: P
authors.lastname: Wittouck
authors.forename: L
authors.initials: L
authors.lastname: Mian
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D006692:Classical Swine Fever Virus / Q000276:immunology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D008214:Lymphocytes / Q000187:drug effects*; D008297:Male; D009152:Mutagenicity Tests; D013552:Swine; D013997:Time Factors; D014613:Vaccines, Attenuated / Q000494:pharmacology*; D014765:Viral Vaccines / Q000494:pharmacology*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines