Cellular immunotherapy of advanced human immunodeficiency virus type 1 infection using autologous lymph node lymphocytes: effects on chemokine production.
A pilot study was undertaken in patients with human immunodeficiency virus type 1 (HIV-1) infection to examine the effects of infusing autologous lymph node lymphocytes that had been cultured ex vivo in conditions designed to maximize the specific secretion of HIV-1-suppressive factors, including beta chemokines. Ten patients with CD4 cell counts between 119 and 436/microliter on antiretroviral drugs received a single infusion of CD4 and CD8 lymph node lymphocytes. There were no serious acute or chronic adverse clinical effects. Increases in serum levels of macrophage inflammatory protein 1beta (MIP-1beta) and increases in the production of MIP-1beta by peripheral blood lymphocytes in response to HIV-1 env were observed. Increases in CD4 and CD8 cell counts and skin test reactivity to recall antigens and decreases in HIV-1 virus load were also observed. This cellular immunotherapy can modulate beta chemokine production in patients with advanced HIV-1 infection and may contribute immunorestorative and antiviral activities.
Triozzi
P L
PL
Department of Medicine, Arthur G. James Cancer Hospital and Research Institute, Columbus, Ohio, USA.
Aldrich
W
W
Bresler
H S
HS
Para
M F
MF
Flancbaum
L J
LJ
D001804:Blood Transfusion, Autologous / Q000009:adverse effects / Q000379:methods; D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D054407:Chemokine CCL4; D018925:Chemokines / Q000096:biosynthesis* / Q000097:blood; D015686:Gene Products, env / Q000276:immunology; D015658:HIV Infections / Q000276:immunology* / Q000628:therapy* / Q000821:virology; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007111:Immunity, Cellular; D007167:Immunotherapy / Q000009:adverse effects / Q000379:methods*; D008198:Lymph Nodes / Q000276:immunology; D017710:Lymphocyte Transfusion / Q000009:adverse effects / Q000379:methods; D019402:Macrophage Inflammatory Proteins / Q000096:biosynthesis / Q000097:blood; D010865:Pilot Projects; D012882:Skin Tests; D013997:Time Factors
D054407:Chemokine CCL4; D018925:Chemokines; D015686:Gene Products, env; D019402:Macrophage Inflammatory Proteins