Quantitative analysis of major histocompatibility complex class II-positive cells in posterior segment of Royal College of Surgeons rat eyes.
Potential antigen-presenting cells in the posterior segment of Royal College of Surgeons (RCS) rat eyes were analyzed quantitatively. Light microscopic immunohistochemistry was performed at postnatal days (P) 10, 20, 28, 42, 63, and 140 in the eyes of RCS rats and their congenic counterparts. Immunohistochemical studies were carried out using monoclonal antibodies against major histocompatibility complex (MHC) class II antigen (OX6), a cytoplasmic antigen in bone marrow-derived macrophages (ED1), a membrane antigen on resident tissue macrophages (ED2), and a microglia/macrophage marker (OX42). Some sections were stained by a double-labeling method using these antibodies. No MHC class II-positive cells were seen in dystrophic RCS rat retinas at P10. They were found, however, in the outer nuclear layer and debris of outer segments at P20. From P20 to P42 the number of cells increased, then decreased until P140. Congenic controls, however, showed no MHC class II-positive cells in the retina. Cells double-labeled with OX6 and ED1 were present in the outer nuclear layer at P42, but no OX6 or OX42 double-labeled cells were detected. Also, no ED2-positive cells were detected. Our results suggest that MHC class II-positive cells may play some role in retinal dystrophy.
Akaishi
K
K
Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan.
Ishiguro
S
S
Durlu
Y K
YK
Tamai
M
M
D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D017628:Microglia / Q000378:metabolism* / Q000473:pathology; D020419:Photoreceptor Cells, Vertebrate / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011922:Rats, Mutant Strains; D012162:Retinal Degeneration / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D000949:Histocompatibility Antigens Class II