Membrane carbohydrate conjugates desialylation does not alter [3H]-dopamine uptake in rat striatal slices.
Incubation of rat striatal slices induced a large decrease (about 50%) of DA uptake and a slight desialylation of polysialogangliosides (GT1b, GD1b, GD1a) with an increase of monosialogangliosides (GM1). Moreover, a pretreatment of slices by exogenous added neuraminidase of Vibrio cholerae did not modify DA uptake, although the pattern of gangliosides was modified and there was considerable loss (about 45%) of sialic acid in gangliosides and glycoproteins. It was verified that neuraminidase activity occured in synaptic membrane. Thus, DA uptake was apparently not altered by desialylation of plasma membrane carbohydrate conjugates.
Page
G
G
Centre d'Etudes et de Recherche sur les Xénobiotiques, UPRES EA 1223, Faculté de Médecine et de Pharmacie, Poitiers, France.
Barrier
L
L
Fauconneau
B
B
Piriou
A
A
Tallineau
C
C
Huguet
F
F
D000818:Animals; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism*; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D004298:Dopamine / Q000493:pharmacokinetics*; D005677:G(M1) Ganglioside / Q000378:metabolism; D005732:Gangliosides / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D009439:Neuraminidase / Q000032:analysis / Q000494:pharmacology; D009994:Osmolar Concentration; D051381:Rats; D017208:Rats, Wistar; D014316:Tritium; D014734:Vibrio cholerae / Q000737:chemistry
D005732:Gangliosides; D006023:Glycoproteins; C025447:sialogangliosides; D014316:Tritium; D005677:G(M1) Ganglioside; D009439:Neuraminidase; D019158:N-Acetylneuraminic Acid; D004298:Dopamine