Identification of the 2B4 molecule as a counter-receptor for CD48.
The CD48 molecule belongs to a subfamily of the Ig superfamily that also includes the CD2, CD58, 2B4, Signaling lymphocyte activation molecule (SLAM), and Ly-9 molecules. Receptor-ligand interactions are known to occur between several members of this family, and these interactions can strengthen cell to cell adhesion. In mice, the CD48 molecule can bind to CD2. To search for additional ligands of murine CD48, we have generated a chimeric fusion protein consisting of the extracellular domain of murine CD48 and the C region of human IgG1. The results of immunofluorescence and immunoprecipitation experiments in which this reagent was used identify the 2B4 molecule as a novel counter-receptor of CD48.
Latchman
Y
Y
Department of Immunology, Imperial College School of Medicine, Hammersmith Hospital, London, United Kingdom.
McKay
P F
PF
Reiser
H
H
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D018801:CD2 Antigens / Q000378:metabolism; D000071178:CD48 Antigen; D019556:COS Cells; D002460:Cell Line; D006801:Humans; D007074:Immunoglobulin G / Q000235:genetics; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins / Q000138:chemical synthesis / Q000276:immunology / Q000378:metabolism; D000071176:Signaling Lymphocytic Activation Molecule Family; D013194:Staining and Labeling; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000378:metabolism; D014162:Transfection
D015703:Antigens, CD; D018801:CD2 Antigens; C084093:CD244 protein, human; D000071178:CD48 Antigen; C000604065:CD48 protein, human; C490007:Cd244a protein, mouse; C000604066:Cd48 protein, mouse; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008024:Ligands; D008562:Membrane Glycoproteins; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins; D000071176:Signaling Lymphocytic Activation Molecule Family