title: Development of avian tibial dyschondroplasia: gene expression and protein synthesis.
abstract: Age-dependent gene expression and protein synthesis associated with chondrocyte differentiation were evaluated in the epiphyseal growth plates of normal and tibial dyschondroplasia (TD)-afflicted chickens. In the normal growth plate, collagen type II gene is expressed mainly by chondrocytes at the upper zone of the growth plate and by the chondrocytes in the articular cartilage. Collagen type X and osteopontin (OPN) genes are expressed in the lower zone of the growth plate and in the zone of cartilage-to-bone transition. No age-dependent changes in the pattern of OPN and collagen type II or X gene expression were observed up to 20 days of age. In the TD-afflicted growth plates, the lesion is enlarged with age, and chondrocytes expressing the collagen type II gene were observed in the hypertrophic zone as early as 8 days posthatching. Abnormal expression of OPN and collagen type X genes was also observed starting at 13 days of age. At day 20, the entire TD lesion-which was significantly enlarged-was surrounded by collagen type II, collagen type X, and OPN expressing cells. The level of OPN in TD was reduced with increasing age, and at 20 days almost no OPN could be detected in either the upper or the lower hypertrophic zones. The level of bone sialoprotein (BSP) also diminished with increasing age in the TD growth plates. In contrast to OPN, the age-dependent reduction in BSP levels was mainly in the lower hypertrophic zone (LHZ), and at 20 days of age, BSP was barely detected in the LHZ, whereas in the upper hypertrophic zone, the levels of BSP were similar to those in normal growth plate. In summary, our results suggest that the primary event of the TD lesion occurs in cells of proliferative phenotype within the hypertrophic zone. These cells divide and form the TD lesion, which consists of cells that do not express the genes associated with hypertrophy.
authors.affiliation: Institute of Animal Science, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel.
authors.forename: M
authors.initials: M
authors.lastname: Pines
authors.forename: V
authors.initials: V
authors.lastname: Knopov
authors.forename: O
authors.initials: O
authors.lastname: Genina
authors.forename: S
authors.initials: S
authors.lastname: Hurwitz
authors.forename: A
authors.initials: A
authors.lastname: Faerman
authors.forename: L C
authors.initials: LC
authors.lastname: Gerstenfeld
authors.forename: R M
authors.initials: RM
authors.lastname: Leach
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002645:Chickens; D019902:Chondrocytes / Q000378:metabolism / Q000473:pathology; D003094:Collagen / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D010006:Osteoblasts / Q000378:metabolism / Q000473:pathology; D010009:Osteochondrodysplasias / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D053495:Osteopontin; D012795:Sialoglycoproteins / Q000096:biosynthesis* / Q000235:genetics; D013977:Tibia / Q000473:pathology* / Q000503:physiopathology
chemical_list: D012795:Sialoglycoproteins; D053495:Osteopontin; D003094:Collagen