Use of ethanolamine for sample stacking in capillary electrophoresis.
Capillary zone electrophoresis (CZE) in the presence of ethanolamine was used in a micropreparative mode. Sample volumes up to 1 microL could be loaded onto a 100 microns diameter capillary without loss in resolution. Coupled to narrow-bore reversed-phase high-performance liquid chromatography, ethanolamine-CZE allowed the collection of sufficient amounts of pure peptidic material to perform amino acid sequence analysis.
Louwagie
M
M
Laboratoire de Chimie des Protéines, CEA/Grenoble, France.
Rabilloud
T
T
Garin
J
J
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary / Q000379:methods*; D019856:Ethanolamine; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism; D017421:Sequence Analysis; D014357:Trypsin / Q000378:metabolism
D007202:Indicators and Reagents; D010446:Peptide Fragments; D011506:Proteins; D019856:Ethanolamine; D014357:Trypsin