Improved protocol for high-throughput cysteine scanning mutagenesis.
Howorka
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S
Department of Medical Biochemistry and Genetics, College of Medicine, Texas A&M University, College Station 77843-1114, USA.
Bayley
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H
D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D003545:Cysteine / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
D001426:Bacterial Proteins; D008565:Membrane Proteins; C104635:SbsB protein, Bacillus stearothermophilus; C048503:ATGCAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D003545:Cysteine