title: Distribution of neuronal intermediate filament proteins in the developing mouse olfactory system.
abstract: The distribution of neuronal intermediate filament proteins in the developing mouse olfactory bulb and olfactory epithelium was characterized by immunocytochemical approach. Antibodies against alpha-internexin, neurofilament triplet proteins (NFTPs; NF-L, NF-M, and NF-H) and peripherin were used to determine their expression at different developmental stages. Alpha-internexin and peripherin were first found to be co-localized in the olfactory neuroepithelium during early development. At the perinatal stage, expression patterns of alpha-internexin and peripherin are distinguishable by spatial and temporal manner: peripherin is predominantly expressed in the olfactory nerves; whereas alpha-internexin is expressed in both olfactory nerves and olfactory bulb. Our observation suggests that peripherin as well as alpha-internexin may play some roles in the process formation of olfactory nerves during development. In the developing olfactory periglomerulus, alpha-internexin was found around postnatal Day 3, whereas NFTPs were not observed until postnatal Day 7. Our data showed that the expression of alpha-internexin preceded those of the NFTPs in most neurons of the developing olfactory bulb. Some small neurons in the adult olfactory bulb were uniquely labeled with antibody to alpha-internexin. Our results suggest that alpha-internexin may play a functional role in the neuronal cytoarchitecture of developing olfactory system, and can be a neuronal marker for detecting postmitotic migrating neurons in the adult olfactory bulb.
authors.affiliation: Department of Anatomy, College of Medicine, National Taiwan University, Taipei, Republic of China.
authors.forename: C L
authors.initials: CL
authors.lastname: Chien
authors.forename: T H
authors.initials: TH
authors.lastname: Lee
authors.forename: K S
authors.initials: KS
authors.lastname: Lu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D018507:Gene Expression Regulation, Developmental; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008562:Membrane Glycoproteins; D051379:Mice; D008813:Mice, Inbred ICR; D009419:Nerve Tissue Proteins / Q000378:metabolism; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D009830:Olfactory Bulb / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D009832:Olfactory Nerve / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D064531:Peripherins; D014746:Vimentin / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D007381:Intermediate Filament Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D016900:Neurofilament Proteins; D064531:Peripherins; D014746:Vimentin; C046643:alpha-internexin