Polymerase chain reaction amplification of Trichomonas vaginalis DNA from Papanicolaou-stained smears.
DNA from Papanicolaou-stained smears was successfully amplified using the polymerase chain reaction (PCR) to investigate if it could be used for retrospective genome studies, such as for the detection of Trichomonas vaginalis. DNA was isolated from 20 archival Papanicolaou-stained smears (19 cervical and 1 seminal fluid samples) and purified by treatment with proteinase K, phenol/chloroform, and ethanol. The method of recovering DNA from the Papanicolaou-stained smear is very important in the successful amplification of Trichomonas DNA in archival cytological smears. We used a specific sequence and successfully amplified DNA in cytological smears of T. vaginalis using PCR. We conclude that the diagnostic accuracy of the detection of T. vaginalis in cervical smears is enhanced by using a combination of a Papanicolaou-stained smear and PCR.
Okuyama
T
T
Second Department of Pathology, Graduate School of Medicine, Kobe University, Hyogo, Japan.
Takahashi
R
R
Mori
M
M
Osaka
M
M
Kobayashi
T K
TK
Maeda
S
S
D000328:Adult; D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D005260:Female; D006801:Humans; D008875:Middle Aged; D065006:Papanicolaou Test; D016133:Polymerase Chain Reaction / Q000379:methods; D012189:Retrospective Studies; D014245:Trichomonas Infections / Q000469:parasitology* / Q000473:pathology; D014246:Trichomonas vaginalis / Q000235:genetics*; D014626:Vaginal Smears
D016054:DNA, Protozoan