title: Lipid and fatty acid composition of different fractions from rat urinary transitional epithelium.
abstract: The phospholipid composition of rat urinary transitional epithelium (TE) and the fatty acid composition of microsomal, mitochondrial, cytosolic, and plasma membrane (PM) subcellular fractions were investigated. PM marker enzymes and electron microscopy analysis were used to characterize the PM fraction, which showed a distinctive lipid composition compared to the general profile of PM from different sources. The levels of cholesterol and sphingomyelin were not enriched in the PM fraction; on the other hand, the increased amounts of glycosphingolipids and phosphatidylserine, and the decreased level of phosphatidylcholine followed the general features of a PM profile. This differential PM lipid composition may reflect the unique morphology of this mammal TE, consisting of concave plaques with an asymmetrical membrane unit. The distribution of the double bond across the PM indicated a higher unsaturation of the inner relative to the outer part of the PM hemileaflet. In addition, the presence of 20:3n-9 nonessential fatty acid in a normal TE may represent a characteristic fatty acid metabolism of this epithelium.
authors.affiliation: Primera Catedra de Histologia, Embriologia y Genetica, Instituto de Biologia Celular, Facultad de Ciencias Medicas, Universidad Nacional de Cordoba, Argentina. aeynard@cmefem.uncor.edu
authors.forename: R O
authors.initials: RO
authors.lastname: Calderon
authors.forename: M
authors.initials: M
authors.lastname: Glocker
authors.forename: A R
authors.initials: AR
authors.lastname: Eynard
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000201:enzymology / Q000648:ultrastructure; D003600:Cytosol / Q000737:chemistry; D005227:Fatty Acids / Q000032:analysis* / Q000737:chemistry; D005260:Female; D008051:Lipid Bilayers; D008055:Lipids / Q000032:analysis* / Q000737:chemistry*; D008297:Male; D008861:Microsomes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D001743:Urinary Bladder / Q000737:chemistry*; D019459:Urothelium / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008051:Lipid Bilayers; D008055:Lipids