Stability of plasma-polymerized allylamine films with sterilization by autoclaving.
The stability of plasma-polymerized allylamine films with autoclaving sterilization cycles was investigated. Polymerized films were deposited under pulsed plasma conditions using two different duty cycles to provide surfaces having different initial amino group concentrations. The film properties were analyzed by XPS and water contact angle measurements before and after autoclaving. The reactions of these surfaces with trifluoroacetic anhydride provided quantitation of the amino surface concentrations before and after autoclaving. In general, the plasma-polymerized films exhibit good stability vis à vis the autoclaving process, with relatively high retention of the surface amino groups. The results of this work are of specific value with respect to tissue culture studies in which surface modifications involving the introduction of amino groups have been shown to have high efficacy in promoting cell growth. The results obtained suggest that the simple one-step plasma treatment process is a viable alternative to the more cumbersome surface modification procedures currently employed to introduce amino groups in these tissue culture studies.
Calderon
J G
JG
Department of Chemistry and Biochemistry, University of Texas at Arlington, 76019-0065, USA.
Harsch
A
A
Gross
G W
GW
Timmons
R B
RB
D000089:Acetic Anhydrides; D000499:Allylamine / Q000031:analogs & derivatives* / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D004355:Drug Stability; D005463:Fluoroacetates; D006358:Hot Temperature; D013242:Sterilization / Q000379:methods; D013499:Surface Properties; D013816:Thermodynamics
D000089:Acetic Anhydrides; D001672:Biocompatible Materials; D005463:Fluoroacetates; D000499:Allylamine; C017958:trifluoroacetic anhydride