Role of DNA definite structural elements in interaction with repair enzyme uracil-DNA glycosylase.
Interaction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil-DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG-substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme-substrate recognition. However the phosphate group adjacent to single dU from the 3'-end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2'-deoxy-2'-aminouridine is a non-hydrolyzable substrate analog for UDG.
Kubareva
E A
EA
A. N. Belozersky Institute of Physical and Chemical Biology, Chemistry Department, Moscow State University, Russia. kubareva@genebee.msu.su
Vasilenko
N L
NL
Vorobjeva
O V
OV
Volkov
E M
EM
Oretskaya
T S
TS
Korshunova
G A
GA
Nevinsky
G A
GA
D004247:DNA / Q000378:metabolism*; D045647:DNA Glycosylases; D004260:DNA Repair; D003857:Deoxyuridine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D006801:Humans; D009699:N-Glycosyl Hydrolases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism* / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D010920:Placenta / Q000201:enzymology; D051981:Uracil-DNA Glycosidase
C116559:2'-deoxy-2'-aminouridine; D009838:Oligodeoxyribonucleotides; D010710:Phosphates; D004247:DNA; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase; D003857:Deoxyuridine