Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human cervical cancers.
OBJECTIVE
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a classical glycolytic protein. A higher level of GAPDH mRNA was found in lung, pancreas, and prostate cancers, but in cervical carcinoma there have not been any reports about the level of GAPDH gene expression. So, we tried to investigate the GAPDH gene expression patterns in cervical carcinomas compared to normal cervical tissues, and the relationships between the expression levels of this gene and conventional clinicopathological parameters were evaluated.


METHODS
In this study, 25 normal exocervical tissues, 35 primary untreated cervical cancer tissues, 2 cervical cancer cell lines, and 2 post-nude-mouse-derived cervical cancer cell lines were subjected to Northern blot analyses for GAPDH gene expression.


RESULTS
Northern blot analyses revealed that the levels of GAPDH gene expression were elevated in 34 of 35 (97%) cervical carcinoma tissues and all of the 4 cervical cancer cell lines compared to normal cervical tissues. The levels of GAPDH gene expression were more prominent in rapidly proliferating cervical carcinoma cells. The levels of the GAPDH gene expressions in cervical cancer tissues were not associated with conventional clinicopathological parameters including clinical stage, histological type, and degree of differentiation.


CONCLUSIONS
These results suggest that increased GAPDH gene expression is characteristic of human cervical carcinomas and that rapidly proliferating carcinoma cells express more enhanced GAPDH gene. Future gene therapy using antisense oligodeoxynucleotide directed against GAPDH mRNA might be another therapeutic tool for human uterine cervical carcinoma.
Kim
J W
JW
Department of Obstetrics and Gynecology, Research Institute of Molecular Genetics, Catholic University Medical College, 505 Banpo-dong, Seocho-ku, Seoul, 137-040, Korea.
Kim
S J
SJ
Han
S M
SM
Paik
S Y
SY
Hur
S Y
SY
Kim
Y W
YW
Lee
J M
JM
Namkoong
S E
SE
D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015316:Genetic Therapy; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics*; D006801:Humans; D009367:Neoplasm Staging; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000473:pathology / Q000628:therapy
D005987:Glyceraldehyde-3-Phosphate Dehydrogenases