title: Improved protocol for high-throughput cysteine scanning mutagenesis.
authors.affiliation: Department of Medical Biochemistry and Genetics, College of Medicine, Texas A&M University, College Station 77843-1114, USA.
authors.forename: S
authors.initials: S
authors.lastname: Howorka
authors.forename: H
authors.initials: H
authors.lastname: Bayley
mesh_terms: D019943:Amino Acid Substitution; D001426:Bacterial Proteins / Q000235:genetics; D001665:Binding Sites / Q000235:genetics; D003545:Cysteine / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; C104635:SbsB protein, Bacillus stearothermophilus; C048503:ATGCAT-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; D003545:Cysteine