Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.
Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.
Stano
J
J
Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
Nemec
P
P
Bezáková
L
L
Kákoniová
D
D
Kovács
P
P
Neubert
K
K
Liskova
D
D
Andriamainty
F
F
Micieta
K
K
D002384:Catalysis; D018914:Cells, Immobilized; D018553:Cucumis sativus / Q000201:enzymology*; D004795:Enzyme Stability; D006868:Hydrolysis; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism*
D001616:beta-Galactosidase