Expression of CYP2E1 in human lung and kidney during development and in full-term placenta: a differential methylation of the gene is involved in the regulation process.
Methylation of dinucleotide CG residues located in the 5' end of the CYP2E1 gene has been demonstrated to play a role in the control of gene expression in the human developing liver. This study was undertaken to examine the CYP2E1 RNA content of human lung, kidney and full-term placenta and to determine whether the expression of CYP2E1 was controlled by its methylation status in these tissues. CYP2E1 was expressed at a very low level in the lung and kidney at whatever age, and at a variable level in full-term placentas. The restriction profile of genomic DNA was identical in lung and kidney and corresponded to a heavy methylation of HpaII/MspI sites located within the promoter, the first exon and first intron of the CYP2E1 gene. A different pattern of methylation was obtained in full-term placentas, indicating that CpG residues located in the 5' end of the gene were predominantly but not fully demethylated. However, the variable level of CYP2E1 RNA in full-term placentas suggests the involvement of other elements in the regulation process of CYP2E1 in this tissue.
Vieira
I
I
INSERM U75, CHU Necker, Paris, France.
Pasanen
M
M
Raunio
H
H
Cresteil
T
T
D000328:Adult; D015139:Blotting, Southern; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000032:analysis; D019175:DNA Methylation; D015226:Dinucleoside Phosphates / Q000378:metabolism; D005314:Embryonic and Fetal Development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007231:Infant, Newborn; D007668:Kidney / Q000196:embryology / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000196:embryology / Q000201:enzymology*; D010920:Placenta / Q000196:embryology / Q000201:enzymology*; D011247:Pregnancy; D012313:RNA / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019392:Cytochrome P-450 CYP2E1; D004247:DNA; D015226:Dinucleoside Phosphates; D012313:RNA; C015772:CpG dinucleotide