title: Direct regulation of GnRH transcription by CRF-like peptides in an immortalized neuronal cell line.
abstract: The existence of a CRF-dependent inhibition of GnRH transcription was investigated using a neuronal GnRH-expressing cell line (Gn11) stably transfected with mouse (-611 bp) or chicken (-3000 bp) GnRH promoter/luciferase reporter constructs. The presence of the CRF-R1 receptor was established using a specific CRF-R1 antiserum. After 7 h of incubation, urotensin-I and sauvagine increased the mouse GnRH-reporter bioluminescence by 1.3- and 1.2-fold, respectively, compared with control cells. Subsequently, CRF, urotensin-I and sauvagine decreased luciferase reporter activity to about 60% of the control values after 14 h. Similar trends occurred with the chicken GnRH promoter with UI increasing reporter gene activity 2.4-fold over the controls after 14 h incubation. These data provide additional evidence for the direct regulation of GnRH transcription by CRF-like peptides.
authors.affiliation: School of Biological Sciences, University of Manchester, UK.
authors.forename: D J
authors.initials: DJ
authors.lastname: Tellam
authors.forename: M J
authors.initials: MJ
authors.lastname: Perone
authors.forename: I C
authors.initials: IC
authors.lastname: Dunn
authors.forename: S
authors.initials: S
authors.lastname: Radovick
authors.forename: J
authors.initials: J
authors.lastname: Brennand
authors.forename: J E
authors.initials: JE
authors.lastname: Rivier
authors.forename: M G
authors.initials: MG
authors.lastname: Castro
authors.forename: D A
authors.initials: DA
authors.lastname: Lovejoy
mesh_terms: D029845:Amphibian Proteins; D000818:Animals; D002461:Cell Line, Transformed; D003346:Corticotropin-Releasing Hormone / Q000235:genetics*; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D017930:Genes, Reporter; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics*; D008156:Luciferases; D051379:Mice; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D009479:Neuropeptides / Q000235:genetics; D036361:Peptide Hormones; D010455:Peptides / Q000494:pharmacology; D011401:Promoter Regions, Genetic / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000503:physiopathology; D014158:Transcription, Genetic / Q000502:physiology*; D014162:Transfection; D014579:Urotensins / Q000235:genetics*; D014665:Vasodilator Agents / Q000494:pharmacology
chemical_list: D029845:Amphibian Proteins; D009479:Neuropeptides; D036361:Peptide Hormones; D010455:Peptides; D014579:Urotensins; D014665:Vasodilator Agents; D007987:Gonadotropin-Releasing Hormone; C025727:sauvagine; D003346:Corticotropin-Releasing Hormone; D008156:Luciferases