Parallel production of oligonucleotide arrays using membranes and reagent jet printing.
Here, we present a low-cost method to produce compact arrays using microporous materials and reagent jetting. Oligonucleotides are immobilized on membrane sheets as a series of lines. The membrane sheet is then rolled and bound, and the spiral structure is cut like a "jelly roll" to produce identical arrays. The spiral arrays behave much like larger formats using membranes, and hybridization detection can be accomplished using standard signal-generation mechanisms. The method is particularly useful for producing identical arrays from pre-synthesized oligonucleotides.
Stimpson
D I
DI
Barclay College, Haviland, KS, USA.
Cooley
P W
PW
Knepper
S M
SM
Wallace
D B
DB
D004867:Equipment Design; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes
D015345:Oligonucleotide Probes