title: Presence of epidermal growth factor during in vitro maturation of pig oocytes and embryo culture can modulate blastocyst development after in vitro fertilization.
abstract: The present study examined the effect of epidermal growth factor (EGF) during in vitro maturation (IVM) and embryo culture on blastocyst development in the pig. In experiment 1, cumulus oocyte complexes were cultured in North Carolina State University (NCSU) 23 medium containing porcine follicular fluid, cysteine, hormonal supplements, and with or without EGF (0-40 ng/ml) for 20-22 hr. They then were cultured for an additional 20-22 hr without hormones. After maturation, cumulus-free oocytes were co-incubated with frozen-thawed spermatozoa for 5-6 hr. Putative embryos were transferred to NCSU 23 containing 0.4% BSA and cultured for 144 hr. In experiment 2, oocytes were matured in medium containing 10 ng/ml EGF, inseminated, and putative embryos were cultured in the presence of 0-40 ng/ml EGF. In experiment 3, oocytes were cultured in the presence of 0, 10 and 40 ng/ml EGF to examine the kinetics of meiotic maturation. In experiment 4, 2- to 4-cell and 8-cell to morula stage embryos derived from oocytes matured with 10 ng/ml EGF were transferred to the oviduct and uterus, respectively, of each of three recipient gilts (3 and 4 days post-estrus, respectively). The presence or absence of EGF during IVM did not affect cumulus expansion, nuclear maturation, fertilization parameters, or cleavage rate. However, compared to no addition (21%), presence of 1 (33%) and 10 ng/ml EGF (42%) during IVM increased (P < 0.01) the rate of blastocyst development in a concentration-dependent manner. Compared to 10 ng/ml EGF, higher concentrations (20 and 40 ng/ml) reduced (P < 0.01) blastocyst development in a concentration-dependent manner (35% and 24%, respectively). No difference was observed between no addition and 40 ng/ml EGF (22%). Compared to no addition and 10 ng/ml EGF, a significantly (P < 0.001) higher proportion (25% vs. 55%) of oocytes reached metaphase II stage 33 hr after IVM with 40 ng/ml EGF. However, no difference was observed at 44 hr. Transfer of embryos to six recipient gilts resulted in three pregnancies and birth of 18 piglets. The results show that EGF at certain concentrations in IVM medium can influence the developmental competence of oocytes. However, addition of EGF during the culture of pig embryos derived from oocytes matured in the presence of EGF is without effect. Birth of piglets provides evidence that embryos derived from oocytes matured in a medium containing EGF are viable.
authors.affiliation: Department of Animal Sciences, University of Missouri-Columbia, 65211, USA.
authors.forename: L R
authors.initials: LR
authors.lastname: Abeydeera
authors.forename: W H
authors.initials: WH
authors.lastname: Wang
authors.forename: T C
authors.initials: TC
authors.lastname: Cantley
authors.forename: A
authors.initials: A
authors.lastname: Rieke
authors.forename: R S
authors.initials: RS
authors.lastname: Prather
authors.forename: B N
authors.initials: BN
authors.lastname: Day
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000737:chemistry* / Q000187:drug effects; D046508:Culture Techniques; D004624:Embryo Transfer / Q000662:veterinary; D005314:Embryonic and Fetal Development / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000494:pharmacology; D005307:Fertilization in Vitro / Q000662:veterinary; D007700:Kinetics; D008540:Meiosis / Q000187:drug effects; D009865:Oocytes / Q000737:chemistry / Q000254:growth & development*; D013552:Swine / Q000196:embryology*
chemical_list: D004815:Epidermal Growth Factor