Molecular and biochemical analysis of a 105 kDa Mycoplasma gallisepticum cytadhesin (GapA).
The identification of a gene (gapA) from Mycoplasma gallisepticum with homology to the P1 cytadherence gene of Mycoplasma pneumoniae is reported. The gapA gene is a 2895 bp ORF encoding a protein with a molecular mass of 105 kDa. Nucleotide sequence analysis of the gapA gene revealed 45% homology to the M. pneumoniae P1 gene, 46% homology to the Mycoplasma genitalium MgPa gene and 47% homology to the Mycoplasma pirum P1-like protein gene. It has a 64 mol % A+T content compared to 46, 60 and 72 mol % respectively for the P1, MgPa and the P1-like protein genes. As with the P1 and MgPa genes, gapA is a central gene in a multi-gene operon, but unlike the P1 and MgPa genes, there is only a single copy of gapA in the genome. GapA is a trypsin-sensitive surface-exposed protein. Chicken tracheal-ring inhibition-of-attachment assays, using anti-GapA Fab fragments, resulted in 64% inhibition of attachment. These results indicated that GapA plays a role in cytadherence of M. gallisepticum to host cells.
Goh
M S
MS
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
Gorton
T S
TS
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
Forsyth
M H
MH
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
Troy
K E
KE
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
Geary
S J
SJ
Department of Pathobiology, U-89, 61 N Eagleville Rd, University of Connecticut,Storrs, CT 06269-3089,USA.
D018829:Adhesins, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001422:Bacterial Adhesion; D015139:Blotting, Southern; D005798:Genes, Bacterial; D015151:Immunoblotting; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics / Q000502:physiology*; D009693:Nucleic Acid Hybridization; D016366:Open Reading Frames / Q000235:genetics; D020161:Physical Chromosome Mapping; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D018829:Adhesins, Bacterial; C117938:GapA cytadhesin