title: Assessment of archived paraffin-embedded cervical condyloma tissues for mycoplasma-conserved DNA using sensitive PCR-ELISA.
abstract: Clinically, it is important to detect mycoplasmas because these organisms have been implicated in gastric and ovarian cancer, pneumonia, postabortal fever, pelvic inflammatory disease, pyelonephritis, endometritis, urethritis, perinatal mortality, arthritis, spontaneous abortion, infertility and interference with sperm development and they act as cofactors catalyzing the HIV disease state. Recently, the combined polymerase chain reaction and enzyme-linked immunosorbent assay method targeting the consensus DNA of over 15 species of mycoplasmas was shown to be superior for the detection of mycoplasmas. The objective was to determine if there was an association between mycoplasmas and cervical neoplasia. Cervical tissues, histopathologically categorized by cervical intraepithelial neoplasia (CIN) grade, flat or exophytic, and acanthosis or koilocytotic, were used. The results showed that mycoplasmas DNA were present in 21.4% of the condyloma tissues and in 33.3% of condyloma tissues with CIN. In contrast, mycoplasmas DNA were not detected when there were no CIN. The presence or absence of human papillomavirus (HPV) did not make a difference. Mycoplasmas DNA were present in 40.0 and 12.5% of the exophytic and flat condylomas, respectively. A higher percentage of cervical tissues graded with slight koilocytosis had (P = 0.05) mycoplasmas DNA compared with tissues graded with moderate koilocytosis. The detection of mycoplasmas DNA in archived cervical condyloma tissues with CIN corroborated previous reports of an association between mycoplasmas and CIN. However, the association between mycoplasmas and the presence of HPV could not be made in this study.
authors.affiliation: Department of Gynecology and Obstetrics, Loma Linda University School of Medicine, Loma Linda, California, 92350, USA.
authors.forename: M
authors.initials: M
authors.lastname: Kidder
authors.forename: P J
authors.initials: PJ
authors.lastname: Chan
authors.forename: I M
authors.initials: IM
authors.lastname: Seraj
authors.forename: W C
authors.initials: WC
authors.lastname: Patton
authors.forename: A
authors.initials: A
authors.lastname: King
mesh_terms: D003218:Condylomata Acuminata / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D009174:Mycoplasma / Q000235:genetics*; D016133:Polymerase Chain Reaction; D002577:Uterine Cervical Diseases / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000382:microbiology; D002578:Uterine Cervical Dysplasia / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial