title: A heterogeneity in serum osteocalcin N-terminal fragments in Paget's disease: a comparison with other biochemical indices in pre- and postmenopause.
abstract: An osteocalcin (OC) nitrogen (N)-terminal sandwich enzyme immunoassay that employs anit-N-20 (amino acids 1-20) polyclonal and monoclonal antibodies has been developed. This assay has demonstrated the existence of a heterogeneity in the OC N-terminal fragments observed in the serum of a patient with Paget's disease and a normal child. The elevation of the serum OC N-terminal value that occurs in Paget's disease was considered to be comparable to the serum alkaline phosphatase (ALP) elevations that also occur. The size of the peaks corresponding to N-terminal OC fragments in Paget's serum decreased 3 months after bisphosphonate treatment. Serum levels of OC N-terminals, and other biochemical indices, were determined for 67 premenopausal and 181 postmenopausal Japanese women. Serum OC N-terminal levels increased significantly (41.2%) in postmenopausal women compared with age-matched premenopausal women. These results strongly suggest that serum OC N-terminal levels reflect bone turnover rates when bone resorption is dominant.
authors.affiliation: Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Gorai
authors.forename: K
authors.initials: K
authors.lastname: Hosoda
authors.forename: O
authors.initials: O
authors.lastname: Chaki
authors.forename: Y
authors.initials: Y
authors.lastname: Taguchi
authors.forename: M
authors.initials: M
authors.lastname: Nakayama
authors.forename: K
authors.initials: K
authors.lastname: Yoh
authors.forename: T
authors.initials: T
authors.lastname: Yamaji
authors.forename: H
authors.initials: H
authors.lastname: Minaguchi
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000097:blood; D015415:Biomarkers; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010001:Osteitis Deformans / Q000097:blood* / Q000503:physiopathology; D015675:Osteocalcin / Q000097:blood* / Q000737:chemistry; D010446:Peptide Fragments / Q000097:blood; D017698:Postmenopause; D017697:Premenopause
chemical_list: D015415:Biomarkers; D010446:Peptide Fragments; D015675:Osteocalcin; D000469:Alkaline Phosphatase