title: Seroepidemiologic study of Neospora caninum in dairy herds.
abstract: OBJECTIVE
  To identify risk factors associated with Neospora caninum infection in dairy herds in Québec and to estimate seroprevalence in case and control herds.
  
  
  METHODS
  Herd-based case-control and seroprevalence study.
  
  
  METHODS
  3,059 cows from 24 case and 22 control dairy herds in Québec.
  
  
  METHODS
  Blood samples were obtained from each cow, and sera were tested, using an ELISA, for antibodies to N caninum. Owners of herds answered questionnaires requesting information on demographics and herd management practices. Seroprevalence was compared between case and control herds, using the Mann-Whitney test. Risk factors were compared between case and control herds, using logistic regression.
  
  
  RESULTS
  All case herds and 73% of control herds had at least one seropositive cow. Median seroprevalence was significantly greater among case herds (22.5%) than among control herds (7.5%). Dogs were found most often and in greatest numbers on farms housing case herds compared with control herds during the past 3 years.
  
  
  CONCLUSIONS
  Although the exact role that dogs have in transmission of N caninum in dairy herds needs to be elucidated, dogs should have limited access to barns and cattle.
authors.affiliation: Institut Armand Frappier, Université du Québec, Laval-des-Rapides, Canada.
authors.forename: J
authors.initials: J
authors.lastname: Paré
authors.forename: G
authors.initials: G
authors.lastname: Fecteau
authors.forename: M
authors.initials: M
authors.lastname: Fortin
authors.forename: G
authors.initials: G
authors.lastname: Marsolais
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D016022:Case-Control Studies; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology*; D003048:Coccidiosis / Q000453:epidemiology / Q000662:veterinary*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D016015:Logistic Models; D018688:Neospora / Q000276:immunology*; D016017:Odds Ratio; D011336:Probability; D011792:Quebec / Q000453:epidemiology; D012307:Risk Factors; D016036:Seroepidemiologic Studies; D011795:Surveys and Questionnaires
chemical_list: D000913:Antibodies, Protozoan