[Hepato-protective effect of a plant from the Senegalese pharmacopoeia: Tinospora bakis (Menispermaceae)using and in vitro model].
The aim of this study is to appreciate hepatoprotective properties of lyophilized aqueous extract of Tinospora bakis roots. We used hepatocytes isolated from rats and intoxicated by CCl4. Before and after intoxication cells were treated with different concentrations of Tinospora bakis extract. (1 mg to 4 mg/ml). Protection of cells was evaluated by the decrease of intracellular enzymes release (LDH and ASAT). The impact of time on the cytoprotection was also studied. From each rat we isolated 185.4 millions +/- 71.5 hepatocytes. The percent of ASAT and LDH release was significatively decreased when we compared treated and no treated hepatocytes suggesting cells protection. This cytoprotection is dose independent but is more effective for long course treatment. These results show the direct protection of isolated hepatocytes by Tinospora bakis extract.
Diallo-Sall
A
A
Laboratoire de Pharmacologie/Physiologie, Faculté de Médecine et Pharmacie, UCAD, Dakar.
Niang-Ndiaye
M
M
Ndiaye
A K
AK
Dieng
C
C
Faye
B
B
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D016488:Medicine, African Traditional; D018384:Oxidative Stress; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
D010936:Plant Extracts
Etude de l'effet hepato-protecteur d'une plante de la pharmacopée sénégalaise: Tinospora bakis (Menispermaceae) à partir d'un modèle in vitro.