Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24.
We present the cDNA sequence of human GBF1 along with data on expression pattern and genomic attributes. The deduced polypeptide encodes a putative guanine nucleotide exchange factor of 206.5 kDa, containing a centrally positioned Sec7 domain and a proline-rich region at the extreme C terminus. Its mRNA transcripts are found in 17 tissues and cell lines, likely suggesting a housekeeping role for GBF1p. The gene maps to 10q24, 2.33 cR from D10S540. It is fully contained within YAC 822C1 and covers at most 450 kb. Its Sec7 domain-encoding region harbors four introns.
Mansour
S J
SJ
Department of Cell Biology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
Herbrick
J A
JA
Scherer
S W
SW
Melançon
P
P
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D012333:RNA, Messenger; C066755:Sec7 guanine nucleotide exchange factors