Checking the integrity of albumin after pasteurisation: a fluorescent assay for quantitating monomeric albumin using anthroyloxy-fatty acids analogs.
This study presents a new assay based on the binding of anthroyloxy-stearates to albumin and the change in the fluorescence intensity when these fluorescent probes are bound to the protein. This assay permits the quantification of the albumin monomers present in a mixture of monomers and polymers, and allows to check the integrity of albumin after pasteurisation.
Gallez
B
B
Laboratory of Medicinal Chemistry and Radiopharmacy, Catholic University of Louvain, Brussels, Belgium. Gallez@cmfa.ucl.ac.be
Lambert
D M
DM
D000818:Animals; D002417:Cattle; D005227:Fatty Acids; D005456:Fluorescent Dyes; D006358:Hot Temperature; D006801:Humans; D011487:Protein Conformation; D012709:Serum Albumin / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013229:Stearic Acids; D013242:Sterilization
D005227:Fatty Acids; D005456:Fluorescent Dyes; D012709:Serum Albumin; D013229:Stearic Acids; D012710:Serum Albumin, Bovine; C041458:2-(9-anthroyloxy)stearic acid