[Use of the primed in situ labeling technique for a rapid detection of chromosomes X, 18].
OBJECTIVE
To develop a rapid method for detection of chromosomes.


METHODS
Chromosomes X, 18 were detected by the primed in situ labeling(PRINS) in eight samples of female peripheral blood cultures.


RESULTS
Specific chromosomes were obtained on both metaphase and interphase nuclei. The efficiency of labeling was 84%-92%(mean=89%) in chromosome 18, and 73%-87%(mean=84%) in chromosome X. The pretreatment of slides by proteinase K markedly raised the efficiency of labeling and increased the intensity of signals. PRINS reaction could be performed automatically with a programmable thermocycler in less than one hour.


CONCLUSIONS
The results suggest that PRINS is a fast and specific method for identifying chromosomes. It may be a reliable technique for detecting aneuploid in prenatal diagnosis.
Ni
B
B
Hunan Provincial Key Laboratory of Medical Genetics, Family Planning Institute of Hunan Province, Changsha, Hunan, 410005 P. R. China fpihp@public.cs.hn.cn
Li
H
H
Zou
Y
Y
Wu
S
S
D002887:Chromosomes, Human, Pair 18; D005260:Female; D006801:Humans; D014960:X Chromosome