title: Photoreceptor allografts in a feline model of retinal degeneration.
abstract: BACKGROUND
  Photoreceptor transplants provide a potential means to restore function in a degenerate retina and/or rescue degenerating host photoreceptors by trophic influences. We have examined photoreceptor allografts in the Abyssinian cat model of hereditary photoreceptor degeneration to determine the viability and influence of such transplants on the host retina.
  
  
  METHODS
  Small pieces of 3- to 5-day-old normal kitten retina containing undifferentiated photoreceptors were injected into the subretinal space of adult Abyssinian cats at an early stage of retinal degeneration using standard vitreo-retinal surgical techniques. The retinas were examined by ophthalmoscopy and fundus photography, then by light and electron microscopy at different times after surgery.
  
  
  RESULTS
  Such allografts survive for at least 6 months after surgery. The photoreceptors develop outer segments, invariably in rosettes. The transplants gradually integrate with the host retina but detach the host photoreceptor layer from the retinal pigment epithelium (RPE), which tends to reduce the number of host photoreceptors over the transplant. There is no slowing of the photoreceptor degeneration in neighboring non-detached retina. Inflammation or rejection was not detected.
  
  
  CONCLUSIONS
  Undifferentiated, neonatal photoreceptor allografts survive and develop outer segments in the subretinal space of the Abyssinian mutant feline retina. The allografts gradually integrate with the host neural retina without inducing rejection. In the vicinity of the transplant there is increased loss of host photoreceptors, considered to be due to their detachment from the RPE layer. There is no evidence of any rescue of host photoreceptors elsewhere in this mutant retina.
authors.affiliation: Swedish University of Agricultural Sciences, Department Medicine & Surgery, Uppsala, Sweden.
authors.forename: L
authors.initials: L
authors.lastname: Ivert
authors.forename: P
authors.initials: P
authors.lastname: Gouras
authors.forename: P
authors.initials: P
authors.lastname: Naeser
authors.forename: K
authors.initials: K
authors.lastname: Narfstrom
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002415:Cats; D002470:Cell Survival; D004195:Disease Models, Animal; D005654:Fundus Oculi; D009887:Ophthalmoscopy; D010781:Photography; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology / Q000637:transplantation*; D012160:Retina / Q000473:pathology / Q000601:surgery*; D012162:Retinal Degeneration / Q000473:pathology / Q000601:surgery*; D014184:Transplantation, Homologous