title: An ampD gene in Pseudomonas aeruginosa encodes a negative regulator of AmpC beta-lactamase expression.
abstract: The ampD and ampE genes of Pseudomonas aeruginosa PAO1 were cloned and characterized. These genes are transcribed in the same orientation and form an operon. The deduced polypeptide of P. aeruginosa ampD exhibited more than 60% similarity to the AmpD proteins of enterobacteria and Haemophilus influenzae. The ampD product transcomplemented Escherichia coli ampD mutants to wild-type beta-lactamase expression.
authors.affiliation: Département de biologie médicale, Pavillon Marchand, Université Laval, Ste-Foy, Québec, Canada G1K 7P4.
authors.forename: T Y
authors.initials: TY
authors.lastname: Langaee
authors.forename: M
authors.initials: M
authors.lastname: Dargis
authors.forename: A
authors.initials: A
authors.lastname: Huletsky
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000235:genetics*; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; C062460:AmpD protein, Bacteria; D009238:N-Acetylmuramoyl-L-alanine Amidase; C048795:AmpC beta-lactamases; D001618:beta-Lactamases