Identification and cloning of human mitochondrial translational release factor 1 and the ribosome recycling factor.
The complete sequence of the cDNA for the coding region of human mitochondrial translational release factor 1 has been obtained from human EST clones and 5'RACE. This sequence has been analyzed to provide insights into the relationship between release factors from bacteria and from mammalian mitochondria. The complete cDNA for the human mitochondrial ribosome recycling factor has been assembled using EST clones. This factor has been expressed in Escherichia coli and purified as a His-tagged protein.
Zhang
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Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
Spremulli
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D000595:Amino Acid Sequence; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D010443:Peptide Chain Termination, Translational; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; C071720:MTF1 protein, S cerevisiae; D024101:Mitochondrial Proteins; D011506:Proteins; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; C087267:ribosome releasing factor