Carbon dioxide fixation by reversible pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910.
Pyrrole-2-carboxylate decarboxylase from Bacillus megaterium PYR2910 attains a balanced reaction equilibrium with an equilibrium constant of 0.3-0.4 M. Therefore, the enzyme catalyzes the reverse carboxylation of pyrrole after addition of bicarbonate. For the synthesis of pyrrole-2-carboxylate, the reverse reaction was optimized and the equilibrium was shifted towards the carboxylate. The product yield was 230 mM (25.5 g/l) pyrrole-2-carboxylate from 300 mM pyrrole in a batch reaction and 325 mM (36.1 g/l) from 400 mM pyrrole in a fed-batch reaction, using both whole cells and the purified enzyme in a pH 8.0 reaction mixture with bicarbonate saturation of 1.9 M. Kinetic studies indicated, that bicarbonate is the reactive species used by this carbon dioxide-fixation enzyme.
Wieser
M
M
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Gifu-Yanagido, Japan.
Fujii
N
N
Yoshida
T
T
Nagasawa
T
T
D001410:Bacillus megaterium / Q000201:enzymology*; D002245:Carbon Dioxide / Q000737:chemistry*; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000378:metabolism; D002384:Catalysis; D004790:Enzyme Induction; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000737:chemistry; D019163:Reducing Agents / Q000737:chemistry; D013379:Substrate Specificity; D013696:Temperature
D019163:Reducing Agents; D002245:Carbon Dioxide; D002262:Carboxy-Lyases; C113052:pyrrole-2-carboxylate decarboxylase; D010100:Oxygen