192 IgG-saporin abolishes p75 neurotrophin receptor immunoreactivity in rat SCN.
The rat suprachiasmatic (SCN) contains a dense plexus of low-affinity p75 neurotrophin receptor (p75NTR)-immunoreactivity. In some SCN neurons, p75NTR is co-localized with vasoactive intestinal peptide (VIP). The present study examines the effect of third ventricle administration of 192 IgG-saporin immunotoxin on p75NTR and VIP immunoreactivity in the rat SCN. The 192 IgG-saporin immunotoxin abolished p75NTR immunoreactivity in the SCN. VIP immunoreactivity in the SCN of saporin-lesioned animals was not significantly different from that of control animals. Immunolesions of the p75NTR-ir cell population in the SCN may prove useful in clarifying the role of p75NTR in circadian timing.
Moga
M M
MM
Department of Anatomy, Indiana University School of Medicine, Terre Haute 47809, USA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000633:toxicity*; D001923:Brain Chemistry / Q000187:drug effects; D018678:Cholinergic Agents / Q000633:toxicity*; D002940:Circadian Rhythm / Q000502:physiology; D000922:Immunotoxins / Q000633:toxicity*; D008297:Male; D009699:N-Glycosyl Hydrolases; D051381:Rats; D017207:Rats, Sprague-Dawley; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor / Q000032:analysis* / Q000276:immunology; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D013493:Suprachiasmatic Nucleus / Q000737:chemistry* / Q000187:drug effects; D014660:Vasoactive Intestinal Peptide / Q000032:analysis / Q000276:immunology
C085696:192 IgG-saporin; D000911:Antibodies, Monoclonal; D018678:Cholinergic Agents; D000922:Immunotoxins; D020800:Receptor, Nerve Growth Factor; D017475:Receptors, Nerve Growth Factor; D054789:Ribosome Inactivating Proteins, Type 1; D014660:Vasoactive Intestinal Peptide; D009699:N-Glycosyl Hydrolases; D000076985:Saporins