[New automatic test for the study of platelet response to variations in osmotic pressure: application in the evaluation of the viability of platelet concentrates].
Studying the osmotic resistance or swelling of platelets has often been suggested as a global test to assess the viability of those cells. A number of authors have also analysed the behaviour of platelets in hypotonic media by a variety of complementary methods (cell count, morphology, determinations of substances released, photometric measurement of aggregation induced by aggregating agents, etc). Most studies are currently based on the so-called "osmotic shock response" test, which measures according to time the light transmitted through platelet-rich plasma (PRP) after dilution in distilled water. In this study, the authors describe a new automated and reproducible test using slow dialysis to assess platelet osmotic resistance. The "Fragilimeter", a device initially described by the authors to characterise RBC fragility, has been adapted to the study of platelet osmotic behaviour. The variations in light transmission through a platelet suspension according to NaCl concentration are linked to the change in cellular volume and lysis and characterise the viability of the cells. The results obtained with normal platelets revealed the good reproducibility of the technique. The osmotic resistance is evaluated for two parameters: anticoagulant (citrate, EDTA) and cellular concentration. The test was applied to quality control of stored platelet concentrates for transfusion, prepared with different cell separators.
Gigout
T
T
Service d'angio-hématologie-hémorhéologie, LEMTA, Vandoeuvre-les-Nancy, France.
Didelon
J
J
Latger
V
V
Claise
C
C
Schooneman
F
F
Paulus
F
F
Stoltz
J F
JF
D001792:Blood Platelets / Q000502:physiology*; D002470:Cell Survival; D006801:Humans; D008027:Light; D009997:Osmotic Pressure; D010976:Platelet Count; D011786:Quality Control; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity
Nouveau test automatique d'étude de la réponse des plaquettes aux variations de pression osmotique: application à l'évaluation de la viabilité des concentrés plaquettaires.