Multiple photoreceptors mediate the light-induced reduction of GUS-COP1 from Arabidopsis hypocotyl nuclei.
The subcellular localization of COP1, a key photomorphogenic repressor, is regulated by light in Arabidopsis seedlings. Photoreceptor loss-of-function mutants and dominant gain-of-function overexpression transgenes were both used to analyze the influences of the three photoreceptors, phyA, phyB, and CRY1, on the light-regulated subcellular localization of COP1. Through a semiquantitative analysis of the nuclear abundance of GUS-COP1 in the various genetic backgrounds, the specific roles of the individual photoreceptors have been established. The data suggest that multiple photoreceptors influence the light-regulated subcellular localization of COP1 in white light. Under specific wavelengths of light, phyA, phyB, and CRY1 each play critical roles in mediating far-red, red, and blue light signals, respectively. Our data also support an interdependency between CRY1 and the phytochromes in mediating the light-regulated subcellular localization of COP1 and thus seedling development.
Osterlund
M T
MT
Department of Molecular Cell and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Deng
X W
XW
D017360:Arabidopsis / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D002467:Cell Nucleus / Q000378:metabolism* / Q000528:radiation effects; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins / Q000378:metabolism; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D008027:Light; D010786:Photoreceptor Cells; D017956:Photoreceptor Cells, Invertebrate; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism* / Q000528:radiation effects; D010834:Phytochrome / Q000378:metabolism; D051942:Phytochrome A; D051939:Phytochrome B; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects; D012639:Seeds / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D014157:Transcription Factors; D019076:Transgenes; D044767:Ubiquitin-Protein Ligases
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D002352:Carrier Proteins; D056931:Cryptochromes; D029721:Drosophila Proteins; D005136:Eye Proteins; D005420:Flavoproteins; C497973:PHYA protein, Arabidopsis; C497952:PHYB protein, Arabidopsis; D045322:Photosynthetic Reaction Center Complex Proteins; D051942:Phytochrome A; D010940:Plant Proteins; D043562:Receptors, G-Protein-Coupled; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C119828:cry protein, Drosophila; D010834:Phytochrome; D051939:Phytochrome B; C471983:AT2G32950 protein, Arabidopsis; D044767:Ubiquitin-Protein Ligases; D005966:Glucuronidase