title: Recent advances in site-directed spin labeling of proteins.
abstract: Site-directed spin labeling of proteins is experiencing a phase of rapid technical evolution, application and evaluation. New strategies have been introduced for determining membrane protein topography, electrostatic potentials, the orientation of proteins at membrane surfaces and inter-residue distances. New applications include studies of beta strands, structure mapping using spin-spin interactions, domain motions in soluble proteins and extensive structural analysis of a number of membrane and soluble proteins.
authors.affiliation: Jules Stein Eye Institute, University of California, Los Angeles 90095, USA. hubbellw@jsei.ucla.edu
authors.forename: W L
authors.initials: WL
authors.lastname: Hubbell
authors.forename: A
authors.initials: A
authors.lastname: Gross
authors.forename: R
authors.initials: R
authors.lastname: Langen
authors.forename: M A
authors.initials: MA
authors.lastname: Lietzow
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D013113:Spin Labels; D055672:Static Electricity
chemical_list: D011506:Proteins; D013113:Spin Labels