title: Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions.
abstract: Mechanical stimulation of bone induces new bone formation in vivo and increases the metabolic activity and gene expression of osteoblasts in culture. We investigated the role of the actin cytoskeleton and actin-membrane interactions in the transmission of mechanical signals leading to altered gene expression in cultured MC3T3-E1 osteoblasts. Application of fluid shear to osteoblasts caused reorganization of actin filaments into contractile stress fibers and involved recruitment of beta1-integrins and alpha-actinin to focal adhesions. Fluid shear also increased expression of two proteins linked to mechanotransduction in vivo, cyclooxygenase-2 (COX-2) and the early response gene product c-fos. Inhibition of actin stress fiber development by treatment of cells with cytochalasin D, by expression of a dominant negative form of the small GTPase Rho, or by microinjection into cells of a proteolytic fragment of alpha-actinin that inhibits alpha-actinin-mediated anchoring of actin filaments to integrins at the plasma membrane each blocked fluid-shear-induced gene expression in osteoblasts. We conclude that fluid shear-induced mechanical signaling in osteoblasts leads to increased expression of COX-2 and c-Fos through a mechanism that involves reorganization of the actin cytoskeleton. Thus Rho-mediated stress fiber formation and the alpha-actinin-dependent anchorage of stress fibers to integrins in focal adhesions may promote fluid shear-induced metabolic changes in bone cells.
authors.affiliation: Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA.
authors.forename: F M
authors.initials: FM
authors.lastname: Pavalko
authors.forename: N X
authors.initials: NX
authors.lastname: Chen
authors.forename: C H
authors.initials: CH
authors.lastname: Turner
authors.forename: D B
authors.initials: DB
authors.lastname: Burr
authors.forename: S
authors.initials: S
authors.lastname: Atkinson
authors.forename: Y F
authors.initials: YF
authors.lastname: Hsieh
authors.forename: J
authors.initials: J
authors.lastname: Qiu
authors.forename: R L
authors.initials: RL
authors.lastname: Duncan
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000201:enzymology; D000185:Actinin / Q000494:pharmacology; D000199:Actins / Q000502:physiology; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D002460:Cell Line; D051546:Cyclooxygenase 2; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology*; D019204:GTP-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D019012:Integrin beta1 / Q000378:metabolism; D016023:Integrins / Q000502:physiology*; D007527:Isoenzymes / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology*; D010446:Peptide Fragments / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D015398:Signal Transduction; D013314:Stress, Mechanical; D020743:rhoB GTP-Binding Protein
chemical_list: D000199:Actins; D019012:Integrin beta1; D016023:Integrins; D007527:Isoenzymes; D008565:Membrane Proteins; D010446:Peptide Fragments; D016760:Proto-Oncogene Proteins c-fos; D000185:Actinin; D015638:Cytochalasin D; D051546:Cyclooxygenase 2; D011451:Prostaglandin-Endoperoxide Synthases; D019204:GTP-Binding Proteins; D020743:rhoB GTP-Binding Protein