Concomitant activation of jasmonate and ethylene response pathways is required for induction of a plant defensin gene in Arabidopsis.
Activation of the plant defensin gene PDF1.2 in Arabidopsis by pathogens has been shown previously to be blocked in the ethylene response mutant ein2-1 and the jasmonate response mutant coi1-1. In this work, we have further investigated the interactions between the ethylene and jasmonate signal pathways for the induction of this defense response. Inoculation of wild-type Arabidopsis plants with the fungus Alternaria brassicicola led to a marked increase in production of jasmonic acid, and this response was not blocked in the ein2-1 mutant. Likewise, A. brassicicola infection caused stimulated emission of ethylene both in wild-type plants and in coi1-1 mutants. However, treatment of either ein2-1 or coi1-1 mutants with methyl jasmonate or ethylene did not induce PDF1.2, as it did in wild-type plants. We conclude from these experiments that both the ethylene and jasmonate signaling pathways need to be triggered concomitantly, and not sequentially, to activate PDF1.2 upon pathogen infection. In support of this idea, we observed a marked synergy between ethylene and methyl jasmonate for the induction of PDF1.2 in plants grown under sterile conditions. In contrast to the clear interdependence of the ethylene and jasmonate pathways for pathogen-induced activation of PDF1.2, functional ethylene and jasmonate signaling pathways are not required for growth responses induced by jasmonate and ethylene, respectively.
Penninckx
I A
IA
F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Kardinaal Mercierlaan 92, B-3001 Heverlee-Leuven, Belgium.
Thomma
B P
BP
Buchala
A
A
Métraux
J P
JP
Broekaert
W F
WF
D000085:Acetates / Q000494:pharmacology*; D000528:Alternaria / Q000472:pathogenicity; D017360:Arabidopsis / Q000187:drug effects* / Q000235:genetics* / Q000382:microbiology; D029681:Arabidopsis Proteins; D003517:Cyclopentanes / Q000494:pharmacology*; D023082:Defensins; D005030:Ethylenes / Q000494:pharmacology*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant / Q000187:drug effects*; D008954:Models, Biological; D009154:Mutation; D054883:Oxylipins; D010269:Paraquat / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics*; D015398:Signal Transduction; D013481:Superoxides / Q000378:metabolism
D000085:Acetates; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D003517:Cyclopentanes; D023082:Defensins; D005030:Ethylenes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013481:Superoxides; C072239:methyl jasmonate; C036216:ethylene; D010269:Paraquat