Inhibition of HIV-1 replication by chimeric phosphorothioate oligodeoxynucleotides applied in free solution.
Oligodeoxynucleotides (ODNs) containing a variable number of 3' and 5' terminal phosphorothioate linkages were applied in free solution to cells infected by HIV-1. ODNs of 28 nt length were applied at up to 5 microM concentration. The ODNs were found to inhibit HIV-1 infection in a dose dependent manner, which correlated with the number of modified linkages (4, 8 and 12, respectively). A target sequence in the HIV-1 rev mRNA, previously reported as sensitive to antisense inhibition by full length phosphorothioate ODNs, only revealed non-sequence dependent inhibition of HIV-1, when tested by these modified chimers.
Lund
O S
OS
Laboratory for Infectious Diseases, Department 144, Hvidovre Hospital, Hvidovre, Denmark.
Hansen
J E
JE
D001483:Base Sequence; D002460:Cell Line; D002678:Chimera / Q000235:genetics; D003588:Cytopathogenic Effect, Viral; D015757:Genes, rev; D015488:HIV Antigens / Q000096:biosynthesis; D015497:HIV-1 / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D006801:Humans; D020319:Oligodeoxyribonucleotides, Antisense / Q000737:chemistry / Q000235:genetics / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D015488:HIV Antigens; D020319:Oligodeoxyribonucleotides, Antisense