title: Cloning and characterization of a 150 kDa microsphere antigen of Theileria parva that is immunologically cross-reactive with the polymorphic immunodominant molecule (PIM).
abstract: To identify the genes encoding novel immunodominant antigens of Theileria parva a lambda gt11 library of piroplasm genomic DNA was immunoscreened with bovine recovery serum and a gene encoding a 150 kDa antigen (p150) was identified. The predicted polypeptide contains an N-terminal secretory signal sequence and a proline-rich region of repeated amino acid motifs. The repeat region is polymorphic between stocks of T. parva in both copy number and sequence, and analysis of the repeat region from 10 stocks of T. parva revealed 5 p150 variants. A monoclonal antibody (mAb) against the T. parva polymorphic immunodominant molecule (PIM) cross-reacted with the recombinant p150. The p150 has sequence homology with a PIM peptide sequence containing the anti-PIM mAb epitope. Immunoelectron microscopy demonstrated that the p150 antigen, like PIM, is located in the microspheres of the sporozoites and is exocytosed following sporozoite invasion of the host lymphocyte. By immunoelectron microscopy p150 was subsequently transiently detectable on the sporozoite surface and in the lymphocyte cytosol. Immunoblotting showed that p150 is also expressed by the schizont stage, but at much lower levels compared to the sporozoite. These results suggest a major role for p150 in the early events of host-sporozoite interaction.
authors.affiliation: International Livestock Research Institute (ILRI), Nairobi, Kenya. R.Skilton@cgnet.com
authors.forename: R A
authors.initials: RA
authors.lastname: Skilton
authors.forename: R P
authors.initials: RP
authors.lastname: Bishop
authors.forename: C W
authors.initials: CW
authors.lastname: Wells
authors.forename: P R
authors.initials: PR
authors.lastname: Spooner
authors.forename: E
authors.initials: E
authors.lastname: Gobright
authors.forename: C
authors.initials: C
authors.lastname: Nkonge
authors.forename: A J
authors.initials: AJ
authors.lastname: Musoke
authors.forename: M
authors.initials: M
authors.lastname: Macklin
authors.forename: K P
authors.initials: KP
authors.lastname: Iams
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan / Q000737:chemistry / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D015139:Blotting, Southern; D015153:Blotting, Western; D002417:Cattle; D003001:Cloning, Molecular; D003429:Cross Reactions; D016054:DNA, Protozoan / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006790:Host-Parasite Interactions; D016056:Immunodominant Epitopes / Q000276:immunology*; D016253:Microscopy, Immunoelectron; D008863:Microspheres; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic / Q000276:immunology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D016797:Theileria parva / Q000235:genetics / Q000276:immunology*; D013801:Theileriasis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D016056:Immunodominant Epitopes; D011994:Recombinant Proteins