title: Populations of hydrophobic amino acids within protein globular domains: identification of conserved "topohydrophobic" positions.
abstract: The 3D structural comparison of families of divergent homologous domains revealed two main populations of hydrophobic amino acids, one with a low and the other with a significantly higher mean solvent accessibility, allowing two regions of the core of protein globular domains to be distinguished. The side chains of hydrophobic amino acids in topologically conserved positions (positions in the structural alignment where only hydrophobic amino acids are found), which we call topohydrophobic positions, are considerably less dispersed than those of the other amino acids (hydrophobic or not). Mean distances between gravity centers of amino acids in topohydrophobic positions are significantly shorter than those for non-topohydrophobic positions and show that the corresponding amino acids are almost all in direct contact in the inner core of globular domains. This study also showed that the small number of topohydrophobic positions is a characteristic of the structural differences between proteins of a family. This criterion is independent of the sequence identity between the sequences and of the root-mean-square distance between their corresponding structures. Using sensitive sequence alignment processes it will be possible, for many protein families, to identify topohydrophobic positions from sequences only.
authors.affiliation: Systèmes Moléculaires et Biologie Structurale, Laboratoire de Minéralogie-Cristallographie, Paris, France. poupon@lmcp.jussieu.fr
authors.forename: A
authors.initials: A
authors.lastname: Poupon
authors.forename: J P
authors.initials: JP
authors.lastname: Mornon
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000596:Amino Acids / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D012997:Solvents; D014764:Viral Proteins / Q000737:chemistry
chemical_list: D000596:Amino Acids; D011506:Proteins; D012997:Solvents; D014764:Viral Proteins