title: Repair of (6-4) photoproducts in cultured goldfish cells at confluence or treated with H2O2.
abstract: Previously we reported that fluorescent light (FL) illumination prior to UV irradiation (FL preillumination) had multiple effects on the repair of cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts ([6-4] PD) in cultured goldfish cells (RBCF-1) at the exponentially growing phase. In this study, it is shown that even under the confluent condition of RBCF-1 cells, FL preillumination increased the disappearance of (6-4) PD in the dark. In addition, both at confluence and at the exponentially growing phase, the disappearance of (6-4) PD after PR treatment was increased by FL preillumination to RBCF-1 cells. Moreover, it was found that H2O2 pretreatment, followed by UV irradiation, of the exponentially growing cells also enhanced the disappearance of (6-4) PD in the dark and by photorepair treatment. The degree of enhancement by H2O2 pretreatment was almost the same as that by FL preillumination.
authors.affiliation: Department of Biological Sciences, University of Tokyo, Japan. nuchida@aichi-cc.pref.aichi.jp
authors.forename: N
authors.initials: N
authors.lastname: Uchida
authors.forename: H
authors.initials: H
authors.lastname: Mitani
authors.forename: A
authors.initials: A
authors.lastname: Shima
mesh_terms: D000818:Animals; D002460:Cell Line; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D003624:Darkness; D006054:Goldfish; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D011740:Pyrimidine Dimers; D012867:Skin; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers; C058406:pyrimidine-pyrimidone dimer; D004247:DNA; D006861:Hydrogen Peroxide