title: Quantitative assay for epinephrine in dental anesthetic solutions by capillary electrophoresis.
abstract: A simple and robust method for the separation and quantification of epinephrine in dental anesthetic solutions was developed. The method allows the direct injection of high salt solutions without sample pre-treatment. Large sample plugs (5.7% of the total capillary length) are used for epinephrine determination by selective analyte focusing in capillary electrophoresis. The concentration detection limit for epinephrine is about 5.0 x 10(-7) M (90 ng ml-1) with a commercial UV detector. The separation protocol was validated in terms of its precision, linearity, accuracy and specificity.
authors.affiliation: Department of Chemistry, University of British Columbia, Vancouver, Canada.
authors.forename: P
authors.initials: P
authors.lastname: Britz-Mckibbin
authors.forename: A R
authors.initials: AR
authors.lastname: Kranack
authors.forename: A
authors.initials: A
authors.lastname: Paprica
authors.forename: D D
authors.initials: DD
authors.lastname: Chen
mesh_terms: D000766:Anesthesia, Dental; D000779:Anesthetics, Local / Q000737:chemistry*; D004837:Epinephrine / Q000032:analysis*; D006801:Humans; D014662:Vasoconstrictor Agents / Q000032:analysis*
chemical_list: D000779:Anesthetics, Local; D014662:Vasoconstrictor Agents; D004837:Epinephrine