Relationship between platinum-DNA adducts in leukocytes of patients with advanced germ cell cancer and survival.
Platinum-DNA adducts can be measured in peripheral blood leukocytes during platinum-based chemotherapy, and high adduct levels have been correlated with favorable clinical response in patients with germ cell cancer. Twenty-five patients with advanced germ cell cancer were treated with platinum-based chemotherapy regimens using the same dose and schedule of cisplatin. Platinum-DNA adducts were measured by atomic absorption spectrometry on the first and fifth days of the first cycle of cisplatin-based therapy. The patients were followed prospectively for 6-35 months (median, 26 months). Twenty-two patients had adduct levels measured 24 h after the first dose of cisplatin. There was no difference in the mean adduct levels of those who were alive and without progression of disease compared to those who were dead or progressing (P = 0.65). Twenty-three patients had day 5 adduct levels measured. The mean day 5 adduct level in the 15 patients who were alive and without progression was 62.133 fmol/microgram compared to 153.50 fmol/microgram in the patients who were dead or progressing (two-sided P = 0.02). Contrary to previous reports, these data indicate that high platinum-DNA adduct levels do not correlate with favorable outcome in patients with advanced germ cell cancer.
Fisch
M J
MJ
Department of Medicine, Indiana University School of Medicine, and Richard L. Roudebush Veterans Affairs Medical Center, Indianapolis, Indiana 46202, USA.
Howard
K L
KL
Einhorn
L H
LH
Sledge
G W
GW
D000293:Adolescent; D000328:Adult; D002945:Cisplatin / Q000378:metabolism / Q000627:therapeutic use*; D018736:DNA Adducts / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D018237:Germinoma / Q000188:drug therapy* / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010984:Platinum / Q000378:metabolism*; D011446:Prospective Studies; D013736:Testicular Neoplasms / Q000188:drug therapy* / Q000378:metabolism
D018736:DNA Adducts; D010984:Platinum; D002945:Cisplatin