Inflammatory cytokine production in whole blood modified by liposome-encapsulated hemoglobin.
The effect of Neo Red Cells (NRC), liposome-encapsulated hemoglobin, on production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) were studied in whole blood preparations ex vivo. Venous blood was collected with heparin and incubated in a CO2 incubator. Treatment of blood samples with NRC reduced the constitutive levels of TNF-alpha and IL-6. Lipopolysaccharide (LPS) treatment for 24 h increased production of TNF-alpha and IL-6 in a dose-dependent manner. Pretreatment with NRC (5%) for 24 h markedly potentiated the LPS-induced TNF-alpha production and, that of IL-6 to a lesser extent. Northern blotting analysis of total RNA in whole blood showed that pretreatment with NRC caused a marked increase in TNF-alpha mRNA expression in response to LPS. It is concluded that NRC potentiates LPS-induced TNF-alpha and IL-6 production in whole blood ex vivo, and that the potentiating effect of NRC on LPS-induced TNF-alpha production can be attributed, at least in part, to an increase in its mRNA expression.
Niwa
K
K
Hokkaido Red Cross Blood Center, Sapporo, Japan.
Ikebuchi
K
K
Fujihara
M
M
Abe
H
H
Wakamoto
S
S
Ito
T
T
Yamaguchi
M
M
Sekiguchi
S
S
D000328:Adult; D001672:Biocompatible Materials / Q000494:pharmacology; D001802:Blood Substitutes / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood*; D006454:Hemoglobins / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000097:blood; D008081:Liposomes / Q000494:pharmacology; D013997:Time Factors
D001672:Biocompatible Materials; D001802:Blood Substitutes; D016207:Cytokines; D006454:Hemoglobins; D008081:Liposomes