[Isolation of anti-Cryptococcus neoformans immunoglobulin G].
Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.
Illnait Zaragozí
M T
MT
Instituto de Medicina Tropical Pedro Kourí.
Martínez Machín
G
G
Fernández Andreu
C
C
D000818:Animals; D000908:Antibodies, Fungal / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000276:immunology*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D011817:Rabbits
D000908:Antibodies, Fungal; D007074:Immunoglobulin G
Obtención de inmunoglobulina G anti-Cryptococcus neoformans.