PCR methods and plasmid profile analysis for characterisation of Histophilus ovis strains.
The value of polymerase chain reaction (PCR)-based DNA fingerprinting and plasmid profile analysis for differentiation of Histophilus ovis isolates was assessed. Nineteen isolates of H. ovis were typed by PCR-ribotyping, repetitive extragenic palindromic element (REP)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR. These methods distinguished five types by PCR-ribotyping, 11 types by REP-PCR and seven types by ERIC-PCR. The ribotyping method produced a relatively simple pattern and a small number of distinct types and was useful for differentiation of H. ovis from the phenotypically similar organism, Haemophilus somnus. REP- and ERIC-PCR both produced complex banding patterns, but increased the discrimination between strains. Plasmids were found in 12 of the 19 isolates and there were four different plasmid profiles. A combination of the PCR methods and plasmid profile analysis provided a high resolution typing method for H. ovis.
Appuhamy
S
S
Division of Infection and Immunity, University of Glasgow.
Low
J C
JC
Coote
J G
JG
Parton
R
R
D000818:Animals; D015373:Bacterial Typing Techniques / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D016172:DNA Fingerprinting / Q000379:methods / Q000662:veterinary*; D004269:DNA, Bacterial / Q000032:analysis*; D005260:Female; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics*; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D008297:Male; D010413:Penis / Q000382:microbiology; D010957:Plasmids / Q000737:chemistry / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012661:Semen / Q000382:microbiology; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
D004269:DNA, Bacterial