title: Immunolocalization of phospholipase C isoforms in rat kidney.
abstract: BACKGROUND
  Phospholipase C (PLC) is an important factor in signal transduction because this enzyme is activated by several hormones and growth factors. Eight PLC isoforms have been described raising the possibility that different cells express a single isoform or activate specific isoforms in different cells. Therefore, the goal of this study was to determine which PLC isoforms are expressed in specific regions of rat kidney.
  
  
  METHODS
  Western blot analysis was performed in microdissected nephron segments of rat kidney, while immunohistochemical analysis was performed on whole rat kidney slices using PLC isoform-specific antibodies.
  
  
  RESULTS
  All three families of PLC isoforms (beta, gamma, and delta) were present throughout the cortical and medullary regions of the kidney. Only the PLC-beta1 isoform was observed in the brush border of the proximal tubule, but all isoforms were present in glomeruli and in the cytoplasm of tubular epithelial cells. In addition, only the PLC-gamma1 isoform was expressed in the internal elastic lamina of the renal artery, while vasa recta expressed PLC-beta1 most intensely. Medullary thick ascending limbs showed an intense level of expression of all three isoforms.
  
  
  CONCLUSIONS
  Multiple PLC isoforms are present in glomeruli, renal tubules, and renal vasculature in vivo, but with some segment-specific differences. These findings suggest that the response of a specific cell is not determined by expression of only one PLC isoform, with the exception of the brush border of the proximal tubule and the renal arteries. Instead, the presence of multiple PLC isoforms in specific regions of the kidney suggests that hormonal regulation in vivo involves mechanisms beyond cell-specific isoforms of PLC.
authors.affiliation: Renal Division, Department of Medicine, Department of Pathology, and The Center for Cell and Molecular Signaling, Emory University School of Medicine, Atlanta, Georgia, USA.
authors.forename: J P
authors.initials: JP
authors.lastname: Lea
authors.forename: D
authors.initials: D
authors.lastname: Ertoy
authors.forename: J L
authors.initials: JL
authors.lastname: Hollis
authors.forename: M B
authors.initials: MB
authors.lastname: Marrero
authors.forename: J M
authors.initials: JM
authors.lastname: Sands
mesh_terms: D000818:Animals; D015153:Blotting, Western; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D007668:Kidney / Q000201:enzymology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D010738:Type C Phospholipases / Q000032:analysis* / Q000276:immunology
chemical_list: D007527:Isoenzymes; D010738:Type C Phospholipases