Evaluation of adsorption preconcentration/capillary zone electrophoresis/nanoelectrospray mass spectrometry for peptide and glycoprotein analyses.
The use of an on-line adsorption preconcentrator coupled with capillary zone electrophoresis/nanoelectrospray mass spectrometry (PC/CZE/nESMS) is described for the analysis of peptides and protein digests. The investigation was focused on the production of disposable preconcentrators made of large particle size (40 microns irregular packing), thereby eliminating the use of a retaining frit without loss of performance. These preconcentration devices were made of commercially available components which can be easily interfaced to current CZE/nESMS systems. Practical issues such as the composition of the stationary phase, the elution volume and sample breakthrough and carry-over were evaluated in order to optimize the analytical performance of this technique. Under optimized elution conditions, the PC/CZE/nESMS technique provided separation efficiencies in excess of 100,000 theoretical plates for a sample loading of 8 microliters. Sample carry-over was minimized by proper reconditioning of the preconcentrator prior to the CZE separation. Alternatively, the sample carry-over resulting from small elution volumes could be used advantageously to provide multiple analyses from a single injection of sample. The application of this technique is demonstrated for the analysis of proteolytic peptides from a Bauhinia purpurea lectin at a concentration level of 30 nM. Further structural information was obtained using on-line tandem mass spectrometry to elucidate the structure of N-linked glycans and the amino acid sequences of the glycopeptides.
Bateman
K P
KP
Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
White
R L
RL
Thibault
P
P
D000327:Adsorption; D019075:Electrophoresis, Capillary; D006023:Glycoproteins / Q000032:analysis*; D006868:Hydrolysis; D007202:Indicators and Reagents; D037102:Lectins / Q000032:analysis; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D037121:Plant Lectins; D012639:Seeds / Q000737:chemistry
D006023:Glycoproteins; D007202:Indicators and Reagents; D037102:Lectins; D010455:Peptides; D037121:Plant Lectins