title: Variation in sensitivity, absorption and density of the central rod distribution with eccentricity.
abstract: OBJECTIVE
  To assess the human rod photopigment distribution and sensitivity with high spatial resolution within the central +/-15 degrees and to compare the results of pigment absorption, sensitivity and rod density distribution (number of rods per square degree).
  
  
  METHODS
  Rod photopigment density distribution was measured with imaging densitometry using a modified Rodenstock scanning laser ophthalmoscope. Dark-adapted sensitivity profiles were measured with green stimuli (17' arc diameter, 1 degrees spacing) using a T ubingen manual perimeter. Sensitivity profiles were plotted on a linear scale and rod photopigment optical density distribution profiles were converted to absorption profiles of the rod photopigment layer.
  
  
  RESULTS
  Both the absorption profile of the rod photopigment and the linear sensitivity profile for green stimuli show a minimum at the foveal center and increase steeply with eccentricity. The variation with eccentricity corresponds to the rod density distribution.
  
  
  CONCLUSIONS
  Rod photopigment absorption profiles, retinal sensitivity profiles, and the rod density distribution are linearly related within the central +/-15 degrees. This is in agreement with theoretical considerations. Both methods, imaging retinal densitometry using a scanning laser ophthalmoscope and dark-adapted perimetry with small green stimuli, are useful for assessing the central rod distribution and sensitivity. However, at present, both methods have limitations. Suggestions for improving the reliability of both methods are given.
authors.affiliation: Department of Pathophysiology of Vision and Neuro-Ophthalmology, University Eye Hospital, Tübingen, Germany. ralf.tornow@uni-tuebingen.de
authors.forename: R P
authors.initials: RP
authors.lastname: Tornow
authors.forename: R
authors.initials: R
authors.lastname: Stilling
mesh_terms: D000042:Absorption; D000328:Adult; D016880:Anisotropy; D002452:Cell Count; D003623:Dark Adaptation / Q000502:physiology; D003720:Densitometry; D005584:Fovea Centralis / Q000502:physiology; D006801:Humans; D017948:Retinal Rod Photoreceptor Cells / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D012243:Rhodopsin / Q000032:analysis*; D012684:Sensory Thresholds
chemical_list: D012243:Rhodopsin