title: Beta-galactosidase in immobilized cells of gherkin Cucumis sativus L.
abstract: Cell suspensions of gherkin (Cucumis sativus L.) were permeabilized by Tween-80, and immobilized by glutaraldehyde. Beta-galactosidase showed pH optimum at 4.9 and temperature optimum at 58 degrees C. The enzyme catalysed hydrolysis was linear for 3 h with 60-68% conversion of the substrate. The cells characterized by high beta-galactosidase activity and stability on long-term storage showed valuable technological properties.
authors.affiliation: Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Bratislava, Slovak Republic.
authors.forename: J
authors.initials: J
authors.lastname: Stano
authors.forename: P
authors.initials: P
authors.lastname: Nemec
authors.forename: L
authors.initials: L
authors.lastname: Bezáková
authors.forename: D
authors.initials: D
authors.lastname: Kákoniová
authors.forename: P
authors.initials: P
authors.lastname: Kovács
authors.forename: K
authors.initials: K
authors.lastname: Neubert
authors.forename: D
authors.initials: D
authors.lastname: Liskova
authors.forename: F
authors.initials: F
authors.lastname: Andriamainty
authors.forename: K
authors.initials: K
authors.lastname: Micieta
mesh_terms: D002384:Catalysis; D018914:Cells, Immobilized; D018553:Cucumis sativus / Q000201:enzymology*; D004795:Enzyme Stability; D006868:Hydrolysis; D001616:beta-Galactosidase / Q000032:analysis / Q000378:metabolism*
chemical_list: D001616:beta-Galactosidase