[Filter paper sampling for the detection of anti-hepatitis A IgM antibodies].
The usefulness of the whole sample taking in filter paper for the detection of anti-HAV IgM is described. 168 serum and filter paper samples were simultaneously compared. No alterations were observed in the specificity of the enzyme-linked immunosorbent assay for the catchment of IgM in the 52 samples of the control group or in the other 116 from patients with clinical suspicion of acute viral hepatitis. A sensitivity, specificity and coincidence of 100% was obtained on comparing the filter paper with the dilution 1:500 with the serum. The correlation established between the values of optical density of the positive results to anti-HAV IgM, the filter paper at dilution 1:500 and the serum was of 0.895. On applying this type of sample taking in a group of hospitalized patients, 100% of coindicence with clinical suspicion was attained. Besides, it was proved its usefulness for the diagnosis of a viral hepatitis outbreak. According to our results, it is recommended the introduction of this type of sample taking in the diagnosis of hepatitis A.
Rodríguez Lay
L A
LA
Instituto de Medicina Tropical Pedro Kourí.
Ribas Antúnez
M A
MA
Díaz Mendiondo
B
B
Roque Quintero
A
A
Aragón Rodríguez
U
U
Martínez Casanueva
R
R
Rodríguez Valdés
C
C
D001800:Blood Specimen Collection / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D006506:Hepatitis A / Q000175:diagnosis; D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies / Q000032:analysis*; D006801:Humans; D007075:Immunoglobulin M / Q000032:analysis*; D010209:Paper
D035922:Hepatitis A Antibodies; D006508:Hepatitis Antibodies; D007075:Immunoglobulin M
Toma de muestra en papel de filtro para la detección de anticuerpos IgM antivirus de la hepatitis A.