2-Chloro-1,4-dimethoxybenzene as a mediator of lignin peroxidase catalyzed oxidations.
Poly R478, 4-methoxymandelic acid and oxalic acid were oxidized by lignin peroxidase (LiP) in the presence of the fungal metabolite 2-chloro-1,4-dimethoxybenzene (2Cl-14DMB), whereas no oxidation occurred in the absence of 2Cl-14DMB. These substrates clearly inhibited the consumption of 2Cl-14DMB and the formation of 2-chloro-1,4-benzoquinone from 2Cl-14DMB by LiP. The results suggest that 2Cl-14DMB can replace the function of veratryl alcohol as a redox mediator in lignin peroxidase catalyzed oxidations.
Teunissen
P J
PJ
Department of Food Technology and Nutritional Sciences, Wageningen Agricultural University, The Netherlands. pauline.teunissen@imb.ftns.wau.nl
Field
J A
JA
D000840:Anisoles / Q000378:metabolism*; D000880:Anthraquinones / Q000378:metabolism; D001592:Benzyl Alcohols / Q000378:metabolism; D002722:Chlorobenzenes / Q000378:metabolism*; D008333:Mandelic Acids / Q000378:metabolism; D010070:Oxalates / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D011108:Polymers / Q000378:metabolism; D020072:Polyporales / Q000201:enzymology*
D000840:Anisoles; D000880:Anthraquinones; D001592:Benzyl Alcohols; D002722:Chlorobenzenes; D008333:Mandelic Acids; D010070:Oxalates; D011108:Polymers; C066949:4-methoxymandelic acid; C116014:2-chloro-1,4-dimethoxybenzene; C017971:poly R-478; D010544:Peroxidases; C042858:lignin peroxidase; C042197:veratryl alcohol