Analysis of gene expression in lung and thymus of TCDD treated C57BL/6 mice using differential display RT-PCR.
The differential display RT-PCR technique offers a means to identify genes which are regulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Treatment of female C57BL/6 mice with 10 micrograms TCDD/kg body weight followed by DDTR-PCR analysis with a set of different primers revealed differential expression for a number of mRNAs. RNA slot blot analysis of six of these clones confirmed differential expression. Further characterization of one of these clones obtained from lung tissue demonstrated that the corresponding mRNA is about 1 kb and shows significant TCDD induced expression in Northern blot analysis. A Genbank search of this clone showed no similarity to known sequences.
Donat
S
S
Medical Institute of Environmental Hygiene, Heinrich-Heine University of Düsseldorf, Department of Toxicology, Germany.
Abel
J
J
D000818:Animals; D001483:Base Sequence; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008168:Lung / Q000187:drug effects / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013950:Thymus Gland / Q000187:drug effects / Q000502:physiology
D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger