Cis-acting sequences contributing to expression of the Drosophila affinidisjuncta Adh gene in both larvae and adults.
To characterize the cis-acting sequences required for expression of the alcohol dehydrogenase (Adh) gene of the Hawaiian picture-winged fruit fly Drosophila affinidisjuncta, germ-line transformation was used to introduce altered forms of this gene into Drosophila melanogaster. Genes were constructed lacking regions corresponding to known or putative regulatory elements within the D. melanogaster gene as well as to sequences previously shown to be required for expression of the D. affinidisjuncta gene in the larval fat body as assayed by transient transformation. Measurement of alcohol dehydrogenase (EC 1.1.1.1) activity levels produced by the transfected genes indicates that multiple elements in both the 5' and 3' regions of the gene contribute to expression. The dispersed elements appear to function redundantly to ensure high levels of expression. Moreover, in contrast to what has been reported for other Drosophila Adh genes, some of the regulatory elements influence expression in both larvae and adults. In total, these results reveal a regulatory system in which the transcribed region in imbedded in an extended genomic segment rich in cisacting regulatory information.
McKenzie
R W
RW
Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, KY 40292, USA.
Brennan
M D
MD
D000426:Alcohol Dehydrogenase / Q000235:genetics*; D000818:Animals; D004330:Drosophila / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D007814:Larva / Q000235:genetics; D010957:Plasmids; D013237:Stereoisomerism; D014158:Transcription, Genetic / Q000235:genetics; D014162:Transfection
D000426:Alcohol Dehydrogenase