Cloning and sequencing of the homologues of both the bacterial and eukaryotic initiation factor genes (hIF-2 and heIF-2 gamma) from archaeal Halobacterium halobium.
The cloning and sequencing of the genes encoding the translational initiation factors (hIF-2 and heIF-2 gamma) was performed by screening the halophilic archaeon Halobacterium halobium genomic library with a probe constructed from the peptide IGHVDHGK that is conserved in archaeal GTP-binding elongation factors. The codon usage by the hIF-2 and heIF-2 gamma genes showed a preference for triplets ending in G or C. This characteristic is almost identical to that of other H. halobium genes. The translated protein of hIF-2 and heIF-2 gamma genes is made of 414 and 583 amino acid residues, respectively, and contains the sequence motif for the binding of GTP. The sequence of hIF-2 shows a strong similarity to the initiation factor IF-2 from Bacteria whereas heIF-2 gamma shows a strong similarity to the initiation factor eIF-2 gamma from Eucarya.
Hasegawa
Y
Y
Department of Bioresource Development, Hiroshima Prefectural University, Japan.
Sawaoka
N
N
Kado
N
N
Ochi
M
M
Itoh
T
T
D000595:Amino Acid Sequence; D019843:Archaeal Proteins; D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D003062:Codon; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000235:genetics; D019766:Genes, Archaeal; D015698:Genomic Library; D006160:Guanosine Triphosphate / Q000378:metabolism; D019617:Halobacterium salinarum / Q000235:genetics*; D008969:Molecular Sequence Data; D010448:Peptide Initiation Factors / Q000737:chemistry / Q000235:genetics*; D039881:Prokaryotic Initiation Factor-2; D019642:RNA, Archaeal / Q000737:chemistry / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
D019843:Archaeal Proteins; D003062:Codon; D015852:Eukaryotic Initiation Factor-2; D010448:Peptide Initiation Factors; D039881:Prokaryotic Initiation Factor-2; D019642:RNA, Archaeal; D012333:RNA, Messenger; C116558:eIF-2gamma, Halobacterium halobium; C116557:initiation factor 2, archaeal; D006160:Guanosine Triphosphate