Chlamydia trachomatis antibody detection and diagnosis of reactive arthritis.
OBJECTIVE
To investigate whether determining the presence of serum or synovial fluid (SF) IgG and IgA of anti-Chlamydia antibodies with two recent commercially available enzyme-linked immunosorbent assays (ELISA) using synthetic peptides or recombinant antigen could be helpful to detect possible Chlamydia trachomatis (CT)-involved disease in rheumatological patients without evidence of urogenital CT infection.


METHODS
The prevalence of such antibodies was determined in samples from patients with well-defined disease, i.e. CT sexually acquired arthritis and from patients with other inflammatory arthropathies unrelated to CT.


RESULTS
When considering IgG and/or IgA anti-MOMP or anti-LPS antibodies, a sensitivity of 100% was obtained for serum and SF samples, but with a low specificity. A sensitivity and a specificity equal or close to 80% were observed for the SF IgG anti-MOMP antibodies.


CONCLUSIONS
Clinically, the most appropriate determination was the SF IgG anti-MOMP antibodies. This commercially available ELISA test could be useful for the diagnosis of probable CT reactive arthritis.
Bas
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Department of Internal Medicine, University Hospital, Geneva, Switzerland.
Vischer
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TL
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000276:immunology; D016918:Arthritis, Reactive / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D018272:Porins; D011237:Predictive Value of Tests; D011994:Recombinant Proteins; D013582:Synovial Fluid / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D018272:Porins; D011994:Recombinant Proteins; C071677:omp1 protein, Chlamydia trachomatis