The mini-exon gene of Trypanosoma (Nannomonas) simiae: sequence variation between isolates and a distinguishing molecular marker.
The mini-exon gene repeats from two isolates of Trypanosoma (Nannomonas) simiae were amplified by polymerase chain reaction (PCR). The products from each isolate differed in size, however they cross-hybridised in Southern blots. The nature of the size variation was revealed by comparison of the DNA sequence of each repeat: relative to the BAN7 strain, the mini-exon gene of KETRI 2431 contained three apparent deletions that were flanked by short (> or = 6-bp) direct repeats. Furthermore, one of the cloned repeats was used as a hybridisation probe against DNA from other closely-related African trypanosomes. The lack of hybridisation of the T. (N.) simiae mini-exon gene to genomic DNA from the Forest, Kilifi and Savannah subgroups of T. (N.) congolense and T. (N.) godfreyi indicate that this PCR-hybridisation assay may be useful for distinguishing T. (N.) simiae from other members of the subgenus Nannomonas.
Sturm
N R
NR
Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90095, USA.
Murthy
V K
VK
Garside
L
L
Campbell
D A
DA
D000818:Animals; D001483:Base Sequence; D005091:Exons / Q000235:genetics*; D017125:Genes, Protozoan; D005819:Genetic Markers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D020080:Tandem Repeat Sequences; D014345:Trypanosoma / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
D005819:Genetic Markers