Binding of HIV-2 envelope glycoprotein to CD8 molecules and related chemokine production.
We recently found that human immunodeficiency virus (HIV)-2 envelope glycoprotein, but not that of HIV-1, could bind to CD4 and CD8 molecules on T cells, and that the binding site of HIV-2 envelope glycoprotein was located on the alpha-chain (but not the beta-chain) of CD8. This study showed that the binding of HIV-2 envelope glycoprotein could induce phosphorylation of protein tyrosine kinase p56lck in CD8+ T cells. We also found that production of beta-chemokines in response to HIV-2 envelope glycoprotein was significantly higher than that in response to HIV-1 envelope glycoprotein, and that CD8+ T cells were the main source of beta-chemokines production among the T-cell population. These findings indicate the possibility that the binding of envelope glycoprotein to CD8 molecules are related to signal transduction into CD8+ T cells and the resultant beta-chemokine production in HIV-2 infection. Our results may help to explain the differences in disease manifestations between HIV-1 and HIV-2, including the lower virulence of HIV-2 and the longer survival of HIV-2-infected individuals.
Akimoto
H
H
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Hongo, Bunkyo-ku, Tokyo, Japan.
Kaneko
H
H
Sekigawa
I
I
Hashimoto
H
H
Kaneko
Y
Y
Yamamoto
N
N
D016827:CD8 Antigens / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D054407:Chemokine CCL4; D018946:Chemokine CCL5 / Q000032:analysis / Q000096:biosynthesis; D018925:Chemokines / Q000032:analysis / Q000096:biosynthesis*; D005434:Flow Cytometry; D015699:HIV Envelope Protein gp120 / Q000378:metabolism; D015658:HIV Infections / Q000276:immunology*; D015498:HIV-2; D006801:Humans; D015151:Immunoblotting; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000378:metabolism; D019402:Macrophage Inflammatory Proteins / Q000032:analysis / Q000096:biosynthesis; D010766:Phosphorylation; D011485:Protein Binding; D015398:Signal Transduction / Q000502:physiology*
D016827:CD8 Antigens; D054407:Chemokine CCL4; D018946:Chemokine CCL5; D018925:Chemokines; D015699:HIV Envelope Protein gp120; D019402:Macrophage Inflammatory Proteins; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)