Differentiation dependency of eicosanoid enzyme expression in human tracheobronchial cells.
The expression of 15-lipoxygenase (15-LO) and prostaglandin H synthase-2 (PGHS-2) was studied in retinoic acid (RA) sufficient and RA-deficient normal human tracheobronchial epithelial (NHTBE) cell cultures. In the absence of RA, in which the cultures became squamous metaplastic, neither 15-LO nor PGHS-2 were expressed. In RA-sufficient cultures, which differentiated into a mucociliary epithelium, both enzymes were expressed: PGHS-2 during early phases and 15-LO during late stages of differentiation. Depending on the stage of differentiation, the RA-sufficient cultures produced PGE, and in the presence of exogenous linoleic acid (LA) 13-HODE. Experiments are underway to examine the effects of inflammatory cytokines on eicosanoid metabolism and the role these metabolites play in airway diseases.
Hill
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EM
Laboratory of Molecular Carcinogenesis, NIEHS, Research Triangle Park, NC, USA.
Eling
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Nettesheim
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D001093:Arachidonate 15-Lipoxygenase / Q000096:biosynthesis* / Q000378:metabolism; D001980:Bronchi / Q000166:cytology* / Q000201:enzymology*; D002454:Cell Differentiation / Q000502:physiology; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D015232:Dinoprostone / Q000096:biosynthesis; D004847:Epithelial Cells / Q000166:cytology; D006801:Humans; D006893:Hydroxyeicosatetraenoic Acids / Q000096:biosynthesis; D007527:Isoenzymes / Q000096:biosynthesis / Q000378:metabolism; D008041:Linoleic Acids / Q000096:biosynthesis; D010741:Phospholipases A / Q000096:biosynthesis* / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000096:biosynthesis / Q000378:metabolism; D014132:Trachea / Q000166:cytology* / Q000201:enzymology*; D014212:Tretinoin / Q000378:metabolism / Q000494:pharmacology
D006893:Hydroxyeicosatetraenoic Acids; D007527:Isoenzymes; D008041:Linoleic Acids; C024348:13-hydroxy-9,11-octadecadienoic acid; D014212:Tretinoin; C025984:15-hydroxy-5,8,11,13-eicosatetraenoic acid; D001093:Arachidonate 15-Lipoxygenase; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D015232:Dinoprostone