title: Contribution of breakdown in the regulation of mouse liver glutathione S-transferase subunits during protein depletion and re-feeding.
abstract: The in vivo radioactivity decay of glutathione S-transferase (GST) was observed in livers of normal-fed (N), protein-depleted (D), and re-fed mice (R), labelled with [35S]methionine. Half-lives in days at N, D and R, respectively, were: total GST, 1.7, 1.4, 4.3; Yb1-subunit, 2.0, 1.8, 3.3; Yc-subunit, 2.4, 1.0, 4.2; Yf-subunit, 1. 1, 2.1, 5.0. These findings, together with the fact that the proportions of GST-subunits depend on dietary protein, show that breakdown contributes differentially to control the content of each GST subunit. Data also indicate that GSTs are long-life proteins.
authors.affiliation: Instituto de Investigaciones Biologicas, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, cc 1245, 7600 Mar Del Plata, Argentina.
authors.forename: R
authors.initials: R
authors.lastname: García-Mata
authors.forename: R D
authors.initials: RD
authors.lastname: Conde
authors.forename: P M
authors.initials: PM
authors.lastname: Sanllorenti
mesh_terms: D000818:Animals; D005260:Female; D005502:Food; D015971:Gene Expression Regulation, Enzymologic; D005982:Glutathione Transferase / Q000378:metabolism*; D006207:Half-Life; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008807:Mice, Inbred BALB C; D011488:Protein Deficiency / Q000378:metabolism*; D013217:Starvation
chemical_list: D005982:Glutathione Transferase