Confocal laser scanning microscopy of tumor/vessel relationship in xenografts in nude and scid mice.
Using confocal laser scanning microscopy we studied sections of the T24B, a human bladder carcinoma, grown in C.B.-17 scid/scid or NMRI nu/nu mice in order to examine the relationship between tumor tissue and tumor vessels. Tumor cells were labelled with FITC-anti-cytokeratin and blood vessel endothelia with Cy3-labelled BS-I lectin. In contrast to our expectation, no major leaks in the endothelial lining of blood vessels were observed. We are looking for a suitable marker for mouse lymphatics in order to investigate their possible role.
Rygaard
J
J
Division of Immunology and Cancer, Bartholin Instituttet, Kommunehospitalet, Copenhagen, Denmark.
Engel
A M
AM
Baeksted
M
M
D000818:Animals; D004730:Endothelium, Vascular / Q000648:ultrastructure; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007633:Keratins / Q000032:analysis; D051379:Mice; D008819:Mice, Nude; D016513:Mice, SCID; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D001749:Urinary Bladder Neoplasms / Q000098:blood supply* / Q000648:ultrastructure*
D007633:Keratins