Trimethyltin-induced expression of neuropeptide Y Y2 receptors in rat dentate gyrus.
Trimethyltin (TMT) causes prominent neuronal damage and enhanced expression of neuropeptide Y in the hippocampus. We investigated expression of neuropeptide Y Y2 receptors after TMT intoxication. Markedly elevated (by 470%) concentrations of Y2 receptor mRNA were found in the suprapyramidal blade of the dentate granule cell layer after 5 days. Increases in the infrapyramidal blade were less prominent (by 198%). After 16 days, mRNA levels in both blades of the granule cell layer showed no significant difference from those in controls. Quantification of Y2 receptor-specific binding revealed no significant change at both 5 and 16 days after TMT intoxication. It is suggested, together with a previous report describing a similar increase of neuropeptide Y expression, that a transient expression of Y2 receptors in the dentate gyrus in the initial phase of TMT intoxication may be involved in mediating TMT-induced hippocampal damage.
Sadamatsu
M
M
Department of Pharmacology, University of Innsbruck, Austria.
Tsunashima
K
K
Schwarzer
C
C
Takahashi
Y
Y
Kato
N
N
Sperk
G
G
D000818:Animals; D001345:Autoradiography; D018891:Dentate Gyrus / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D017403:In Situ Hybridization; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000096:biosynthesis*; D014298:Trimethyltin Compounds / Q000494:pharmacology*
D017476:Receptors, Neuropeptide Y; D014298:Trimethyltin Compounds; C095889:neuropeptide Y2 receptor; C046488:trimethyltin