title: Role of interleukin 8 in the genesis of acute respiratory distress syndrome through an effect on neutrophil apoptosis.
abstract: OBJECTIVE
  To evaluate the role of interleukin 8 (IL-8) in the regulation of neutrophil (PMN) apoptosis in normal plasma and plasma from patients with early, fulminant acute respiratory distress syndrome (ARDS).
  
  
  METHODS
  Experimental study using cultured human PMNs.
  
  
  METHODS
  University hospital, level I trauma center.
  
  
  METHODS
  Plasma was obtained from 6 patients with early, fulminant posttraumatic ARDS (mean Injury Severity Score, 26). All samples were drawn within 24 hours after injury. Plasma was also taken from 13 healthy control subjects. These controls were also used as sources of PMNs.
  
  
  METHODS
  Effect of early, fulminant ARDS and normal plasma on spontaneous apoptosis, CD16, and CD11-b expression in PMNs in vitro; levels of IL-8 in plasma; correlation of extracellular IL-8 concentration with rate of PMN apoptosis; and effect of IL-8 blockade on PMN apoptosis, CD16, and CD11-b expression in ARDS and normal plasma.
  
  
  RESULTS
  Plasma from patients with early, fulminant ARDS inhibited spontaneous PMN apoptosis at 24 hours (35%+/-5% vs 54%+/-5%; P=.01). Neither CD16 nor CD1l-b differed significantly between the 2 groups. The mean plasma level of IL-8 in patients with early, fulminant ARDS was 359+/-161 pg/mL vs 3.0+/-0.4 pg/mL in healthy controls (P<.05). Interleukin 8 inhibited apoptosis in plasma-free medium at low doses (1-50 pg/mL) but had no significant effect at higher doses (100-5000 pg/mL) (P<.05). Interleukin 8 blockade with monoclonal antibody suppressed apoptosis in normal plasma (28%+/-5% with monoclonal antibody vs 51%+/-5% without monoclonal antibody; P=.008) but not in plasma from patients with early, fulminant ARDS (29%+/-5% with monoclonal antibody vs 34%+/-6% without monoclonal antibody; P=.67). It had no effect on CD16 or CD11-b expression in either plasma.
  
  
  CONCLUSIONS
  Plasma from patients with early, fulminant ARDS contains soluble factors that inhibit PMN apoptosis in vitro. Low levels of IL-8 inhibit PMN apoptosis in normal plasma. Although plasma levels of IL-8 are markedly elevated in early, fulminant ARDS, IL-8 is not directly responsible for the antiapoptotic effect of plasma from patients with early, fulminant ARDS.
authors.affiliation: Department of Surgery, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103, USA. goodmaer@umdnj.edu
authors.forename: E R
authors.initials: ER
authors.lastname: Goodman
authors.forename: E
authors.initials: E
authors.lastname: Kleinstein
authors.forename: A M
authors.initials: AM
authors.lastname: Fusco
authors.forename: D P
authors.initials: DP
authors.lastname: Quinlan
authors.forename: R
authors.initials: R
authors.lastname: Lavery
authors.forename: D H
authors.initials: DH
authors.lastname: Livingston
authors.forename: E A
authors.initials: EA
authors.lastname: Deitch
authors.forename: C J
authors.initials: CJ
authors.lastname: Hauser
mesh_terms: D017209:Apoptosis / Q000276:immunology*; D018845:CD11 Antigens / Q000276:immunology; D016022:Case-Control Studies; D002478:Cells, Cultured; D006801:Humans; D015601:Injury Severity Score; D016209:Interleukin-8 / Q000097:blood / Q000276:immunology*; D018375:Neutrophil Activation / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D017452:Receptors, IgG / Q000276:immunology; D012128:Respiratory Distress Syndrome / Q000097:blood / Q000209:etiology / Q000276:immunology*; D013997:Time Factors; D014947:Wounds and Injuries / Q000150:complications
chemical_list: D018845:CD11 Antigens; D016209:Interleukin-8; D017452:Receptors, IgG