Stimulation of protein kinase A activity and induced terminal differentiation of human skin fibroblasts in culture by low-frequency electromagnetic fields.
In the present study experiments are described, which indicate that the exposure of normal human skin fibroblasts (HSFs) to a low-frequency electromagnetic field of 20 Hz and 7-8 mT does induce terminal differentiation within 14 days of daily exposure of 12 h. As demonstrated by the analysis of the expression level of the proto-oncogene c-myc, the induction of terminal differentiation of progenitor fibroblasts to postmitotic fibrocytes does most likely not involve changes in the c-myc protein. As one possible candidate being involved in the ELF-EMF-mediated inhibition of fibroblast growth and the subsequent induction of terminal differentiation the cyclic AMP-dependent protein kinase A (PKA) could be characterised. Thus, the data presented clearly indicate that the specific EMF field of 20 Hz and 7-8 mT significantly interfere with regulatory processes of fibroblast proliferation and differentiation.
Löschinger
M
M
Section of Radiobiology and Molecular Environmental Research, University of Tübingen, Germany.
Thumm
S
S
Hämmerle
H
H
Rodemann
H P
HP
D015152:Blotting, Northern; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology / Q000528:radiation effects; D002478:Cells, Cultured; D002675:Child, Preschool; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D004558:Electric Stimulation; D004574:Electromagnetic Fields; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000201:enzymology; D006801:Humans; D008297:Male; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis; D012867:Skin / Q000166:cytology* / Q000201:enzymology*
C000715470:MAS1 protein, human; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D017868:Cyclic AMP-Dependent Protein Kinases