Fusion expression of human pro-urokinase with E. coli thioredoxin.
Human pro-urokinase (pro-UK) was cloned into plasmid pET32b and fused to the E. coli thioredoxin (trxA). When expressed in E. coli AD494(DE3), the fusion protein Trx-pro-UK accumulated as insoluble inclusion bodies and amounted to 35% of total cellular proteins. When co-expressed with molecular chaperones human protein disulfide isomerase (PDI) and E. coli GroESL, all the expressed products still existed in the form of insoluble inclusion bodies.
Sun
A L
AL
Department of Biochemistry, Nanjing University, People's Republic of China.
Hua
Z C
ZC
Yao
J
J
Yang
Y H
YH
Yin
D Q
DQ
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D018833:Chaperonins / Q000096:biosynthesis / Q000235:genetics; D003001:Cloning, Molecular; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D002479:Inclusion Bodies; D008970:Molecular Weight; D019704:Protein Disulfide-Isomerases / Q000096:biosynthesis / Q000235:genetics; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D012995:Solubility; D013879:Thioredoxins / Q000737:chemistry / Q000235:genetics*; D014568:Urokinase-Type Plasminogen Activator / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics
D001426:Bacterial Proteins; D004792:Enzyme Precursors; C097970:GroESL protein, Bacteria; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013879:Thioredoxins; D014568:Urokinase-Type Plasminogen Activator; D018833:Chaperonins; D019704:Protein Disulfide-Isomerases; C067551:saruplase