Ischemic rat brains contain immunoreactivity of 14-3-3 proteins.
14-3-3 proteins and tissue damage association were determined. The localization of 14-3-3 proteins in section of cortex from ischemia produced rats was examined by immunohistochemistry. Ischemia was produced in rats by unilaterally clamping the carotid artery for 6 hrs at the distal side. Following recovery for 6 hrs, the animals were sacrificed. The intensity of neuronal necrosis stained with antibody raised against 14-3-3 protein was markedly more in the cortex than the other parts of the brain, since there was occlusion of the artery which feeds this region. 14-3-3 antibody was confined to neuronal cell body. Since 14-3-3 proteins are central to mitogen-activated protein (MAP) kinase signaling, this pathway is in part responsible for the hyperphosphorylation of neurofilaments or cytoskeletal proteins of neuron, which may possibly lead to neuronal inclusions.
Pirim
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Ataturk University, Medical School, Biochemistry Department, Erzurum, Turkey.
D048948:14-3-3 Proteins; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology*; D002545:Brain Ischemia / Q000378:metabolism*; D002540:Cerebral Cortex / Q000098:blood supply / Q000166:cytology; D004791:Enzyme Inhibitors / Q000032:analysis*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D009410:Nerve Degeneration / Q000378:metabolism; D009474:Neurons / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000235:genetics* / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D000906:Antibodies; D004791:Enzyme Inhibitors; D011506:Proteins; D012333:RNA, Messenger; D014446:Tyrosine 3-Monooxygenase