Conserved structural features between HLA-DO beta and -DR beta.
HLA-DO is a non-classical MHC class II molecule presumed to play a specialized role in the antigen processing pathway. We have modeled the HLA-DO beta-chain and found its overall structure compatible with the one of DR beta. Functional studies further highlighted the similarity between these beta-chains of the class II family of proteins. Indeed, a mixed heterodimer composed of the DR alpha and a chimeric DO beta-chains presented bacterial superantigens to T cells and was shown to interact with CD4. The implications of such structural conservation for the in vivo functions of HLA-DO are discussed.
Thibodeau
J
J
Immunochimie Analytique, Institut Pasteur, Paris, France. thibodj@magellan.umontreal.ca
Lavoie
P M
PM
Samaan
A
A
Corre
J P
JP
Sékaly
R P
RP
Cazenave
P A
PA
D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal; D017951:Antigen Presentation; D002460:Cell Line; D017124:Conserved Sequence; D019281:Dimerization; D006681:HLA-D Antigens / Q000737:chemistry* / Q000235:genetics; D006684:HLA-DR Antigens / Q000737:chemistry* / Q000235:genetics; D000949:Histocompatibility Antigens Class II; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection
D000911:Antibodies, Monoclonal; C416186:H-2O antigen; D006681:HLA-D Antigens; C046148:HLA-DO antigens; D006684:HLA-DR Antigens; D000949:Histocompatibility Antigens Class II; D011993:Recombinant Fusion Proteins