Expression of nuclear hormone receptors in Escherichia coli.
Nuclear hormone receptors are ligand-activated transcription factors. The study of nuclear hormone receptor--ligand interactions requires expression of soluble receptor, but the production of functional soluble receptors in E. coli has generally proved difficult. Recent studies on coexpression of nuclear hormone receptors have yielded active soluble proteins, which were not readily generated when expressed individually.
Mossakowska
D E
DE
Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Essex, UK. Danuta_E_Mossakowska@sbphrd.com
D000818:Animals; D001665:Binding Sites; D001709:Biotechnology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006728:Hormones / Q000378:metabolism*; D006801:Humans; D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D012995:Solubility; D014157:Transcription Factors / Q000235:genetics
D006728:Hormones; D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors