Rapid evaluation in Escherichia coli of antisense RNAs and ribozymes.
The characterization of a simple bacterial system using commercially available plasmids and strains, developed to assess the effectiveness of trans-acting (antisense RNA and ribozymes) RNA in Escherichia coli, is reported. This system was used to test various trans-acting RNA molecules against the expression of the I factor, a functional transposable element from Drosophila melanogaster. For this target, results indicate that antisense RNA efficiency depends on the hybridization length between sense and antisense RNA. The introduction of a single hammerhead ribozyme within the antisense RNAs does not increase its inhibitory activity. These predictions were subsequently confirmed in Drosophila melanogaster.
Tatout
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C
Centre de Génétique Moléculaire et Cellulaire, Université Claude Bernard, Villeurbanne, France.
Gauthier
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E
Pinon
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H
D000818:Animals; D004251:DNA Transposable Elements; D015536:Down-Regulation; D004331:Drosophila melanogaster / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016372:RNA, Antisense / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012270:Ribosomes / Q000235:genetics*; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D016372:RNA, Antisense; D012329:RNA, Bacterial