DNA sequence recognition by bis-linked netropsin and distamycin derivatives.
We studied the interaction of cis-diammine Pt(II)-bridged bis-netropsin, cis-diammine Pt(II)-bridged bis-distamycin and oligomethylene-bridged bis-netropsin with synthetic DNA fragments containing pseudosymmetrical AT-rich nucleotide sequences and compared it with the interaction of the parent compounds netropsin and distamycin A. For fragments containing multiple blocks of (AIT)4 and (T/A)4 separated by zero, one, two and three GC-base pairs, DNase I footprinting and CD spectroscopy studies reveal that 5'-TTTTAAAA-3' is the strongest affinity binding site for cis-diammine Pt(II)-bridged bis-netropsin and bis-distamycin. They both bind less strongly to a DNA region containing the sequence 5'-AAAATTTT-3'. Netropsin, distamycin A and oligomethylene-bridged bis-netropsin exhibit far less sequence discrimination.
Grokhovsky
S L
SL
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.
Surovaya
A N
AN
Burckhardt
G
G
Pismensky
V F
VF
Chernov
B K
BK
Zimmer
C
C
Gursky
G V
GV
D001483:Base Sequence; D001665:Binding Sites; D003001:Cloning, Molecular; D004247:DNA / Q000378:metabolism*; D018983:DNA Footprinting; D003850:Deoxyribonuclease I / Q000378:metabolism; D004214:Distamycins / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009429:Netropsin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism
D004214:Distamycins; D009838:Oligodeoxyribonucleotides; D009429:Netropsin; C032161:bis-netropsin; C030000:stallimycin; D004247:DNA; D003850:Deoxyribonuclease I