[Investigation on the viscoelastic properties of hepatocytes and their relavances to cytoskeleton structure].
Using micropipette aspiration technique, the authors investigated the viscoelastic properties of human fetal hepatocytes (HFH) and hepatocellular carcinoma (HCC) cells. And further, the effects of two cytoskeleton interferents, cytochalasin D and colchicine on the viscoelastic properties of HFHs were investigated. The results showed: the elastic coefficients K1 and K2 of HCC cells were significantly higher than those of HFHs; the action of cytochalasin D led to an obvious decrease in elastic coefficients K1, K2 as well as the viscous coefficient mu of HFHs; under the action of colchicine, the K1 value of HFHs increased while the K2 and mu values decreased obviously or tended to decrease. A brief discussion was presented about these results.
Wu
Z
Z
Bioengineering Research Institute, Chongqing University.
Zhang
G
G
Wang
X
X
Song
G
G
Wang
H
H
Long
M
M
Cai
S
S
Wu
Y
Y
D006528:Carcinoma, Hepatocellular / Q000473:pathology / Q000503:physiopathology*; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology*; D015638:Cytochalasin D / Q000494:pharmacology*; D003599:Cytoskeleton / Q000502:physiology; D004548:Elasticity / Q000187:drug effects; D005333:Fetus; D006801:Humans; D008099:Liver / Q000166:cytology / Q000502:physiology*; D008113:Liver Neoplasms / Q000473:pathology / Q000503:physiopathology*; D014407:Tumor Cells, Cultured / Q000187:drug effects; D014783:Viscosity / Q000187:drug effects
D015638:Cytochalasin D; D003078:Colchicine