title: Selenium-mediated differential response of beta-glucosidase and beta-galactosidase of germinating Trigonella foenum-graecum.
abstract: Beta-glucosidase and beta-galactosidase activity profile tested in different seeds during 24 h germination revealed reasonably high levels of activity in Vigna radiata, Cicer arietinum, and Trigonella foenum-graecum. In all seeds tested, beta-galactosidase activity was, in general, higher than that of beta-glucosidase. T. foenum-graecum seedlings exhibited maximal total and specific activities for both the enzymes during 72 h germination. Se supplementation as Na2SeO3 up to 0.75 ppm was found to be beneficial to growth and revealed selective enhancement of beta-galactosidase activity by 40% at 0.5 ppm Se. The activities of both the enzymes drastically decreased at 1.0 ppm level of Se supplementation. On the contrary, addition of Na2SeO3 in vitro up to 1 ppm to the enzyme extracts did not influence these activities. Hydrolytic rates of beta-glucosidase in both control and Se-supplemented groups were enhanced by 20% with 0.05 M glycerol in the medium and 30% at 0.1 M glycerol. The rates were marginally higher in Se-supplemented seedlings than the controls, irrespective of added glycerol in the medium. In contrast, hydrolysis by beta-galactosidase showed a trend of decrease in Se-supplemented seedlings compared to the control, when glycerol was present in the medium. Addition of Se in vitro in the assay medium showed no difference in the hydrolytic rate by beta-galactosidase when compared to control, while the activity of beta-glucosidase declined by 50%. Se-grown seedlings showed an enhancement of transglucosidation rate by 40% in the presence of 0.1 M glycerol. The study reveals a differential response to Se among the beta-galactosidase and beta-glucosidase of T. foenum-graecum with increase in the levels of beta-galactosidase activity.
authors.affiliation: Department of Chemistry, Indian Institute of Technology, Madras, Chennai.
authors.forename: M
authors.initials: M
authors.lastname: Sreekala
authors.forename: K
authors.initials: K
authors.lastname: Lalitha
mesh_terms: D002384:Catalysis; D018525:Germination; D006868:Hydrolysis; D018521:Plant Physiological Phenomena; D010944:Plants / Q000201:enzymology / Q000378:metabolism*; D012643:Selenium / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
chemical_list: D001617:beta-Glucosidase; D001616:beta-Galactosidase; D012643:Selenium