Calculation of spermatogenic transformations based on dual-flow cytometric analysis of testicular tissue in seasonal breeders.
The percentages of somatic and spermatogenic cells were quantified at different time intervals in testes of three seasonal breeders: roe deer, brown hare and mink. The haploid, diploid and tetraploid cells were monitored by one-parameter flow cytometry. Somatic and spermatogenic cells were distinguished by dual-parameter flow cytometry after DNA staining and selective labelling of the somatic cells with an antivimentin antibody. The portions of spermatogenic diploid and tetraploid cells were used to calculate total germ cell transformation (haploid:diploid cell ratio) as well as meiotic transformation (haploid:tetraploid cell ratio). During the breeding season the means of both ratios were 10.27 and 38.42 in roe deer, 7.55 and 15.81 in brown hare, and 11.25 and 53.47 in mink. The equivalent quotients calculated on the base of total diploid and tetraploid cells were considerably lower: 4.41 and 7.79, 5.13 and 7.86, 5.68 and 13.75, respectively. Portions as well as proportions of germinative and somatic cells changed during the annual cycle. The results demonstrate that the distinction of somatic and germinative cells in flow cytometric studies of spermatogenesis is a prerequisite for exact calculations of mitotic and meiotic processes and their alterations under different conditions.
Blottner
S
S
Institute for Zoo Biology and Wildlife Research, Berlin.
Roelants
H
H
D000818:Animals; D003670:Deer; D005434:Flow Cytometry; D007793:Lagomorpha; D008297:Male; D008907:Mink; D012098:Reproduction; D012621:Seasons; D013091:Spermatogenesis; D013737:Testis / Q000166:cytology*