Cytogenetic effects on lymphocytes in osteoporotic patients on long-term fluoride therapy.
The genotoxicity of fluoride in vivo in seven patients with osteoporosis was cytogenetically investigated. The patients were treated with fluoride-containing formulations (disodium monofluorophosphate and sodium fluoride) for a period of 15 months up to 49 months. Fluoride intake ranged from 22.6-33.9 mg F/day and serum fluoride concentrations were between 0.1 mg F/l and 0.2 mg F/l. Peripheral blood lymphocytes of these patients were cultured in vitro and examined for chromosomal aberrations, micronuclei in cytokinesis-blocked binucleated lymphocytes as well as cell cycle progression. When a comparison was made between patients' group and a matched control group, it was found that fluoride at the tested concentrations had no detectable genotoxic potential in human.
van Asten
P
P
Department of Hospital Pharmacy, Utrecht University Hospital, The Netherlands.
Darroudi
F
F
Natarajan
A T
AT
Terpstra
I J
IJ
Duursma
S A
SA
D000328:Adult; D000368:Aged; D002453:Cell Cycle / Q000187:drug effects; D002869:Chromosome Aberrations; D004311:Double-Blind Method; D005260:Female; D005459:Fluorides / Q000009:adverse effects* / Q000627:therapeutic use; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure*; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects; D015162:Micronucleus Tests; D008875:Middle Aged; D010024:Osteoporosis / Q000097:blood* / Q000188:drug therapy / Q000235:genetics
D005459:Fluorides