[Pathogenesis of disorder due to thyroid hormone receptor mutation].
Thyroid hormone action is mediated through its nuclear receptor (thyroid hormone receptor; TR). A mutation in the TR causes resistance to thyroid hormone (RTH) with autosomal dominant inheritance. Using T3 binding assay, gel shift assay and transfection study, the pathogenesis of this disorder was elucidated at a molecular level. Mutant TRs lose their T3 binding activity, and inhibit wild type TR action in a dominant negative manner. For this dominant negative inhibition, the preservation of DNA binding and dimerization activities are required. It is indicated that the mutant TR complex competes with wild type TR complex for the DNA binding site to exert dominant negative inhibition. Thus, the functional analyses of mutant TRs are useful to elucidate the molecular basis of this disorder.
Nagaya
T
T
Department of Endocrinology and Metabolism, Nagaya University.
Seo
H
H
D006801:Humans; D009154:Mutation; D011988:Receptors, Thyroid Hormone / Q000235:genetics*; D013959:Thyroid Diseases / Q000235:genetics*
D011988:Receptors, Thyroid Hormone