title: Ca2+-dependent exocytosis in mast cells is stimulated by the Ca2+ sensor, synaptotagmin I.
abstract: Mast cells secrete a variety of biologically active substances that mediate inflammatory responses. Synaptotagmin(s) (Syts) are a gene family of proteins that are implicated in the control of Ca2+-dependent exocytosis. In the present study, we investigated the possible occurrence and functional involvement of Syt in the control of mast cell exocytosis. Here, we demonstrate that both connective tissue type and mucosal-like mast cells express Syt-immunoreactive proteins, and that these proteins are localized almost exclusively to their secretory granules. Furthermore, expression of Syt I, the neuronal Ca2+ sensor, in rat basophilic leukemia cells (RBL-2H3), a tumor analogue of mucosal mast cells, resulted in prominent potentiation and acceleration of Ca2+-dependent exocytosis. Therefore, these findings implicate Syt as a Ca2+ sensor that mediates regulated secretion in mast cells to calcium ionophore.
authors.affiliation: Department of Cell Biology and Histology, Sackler School of Medicine, Tel Aviv University, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Baram
authors.forename: M
authors.initials: M
authors.lastname: Linial
authors.forename: Y A
authors.initials: YA
authors.lastname: Mekori
authors.forename: R
authors.initials: R
authors.lastname: Sagi-Eisenberg
mesh_terms: D000818:Animals; D015153:Blotting, Western; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology*; D002135:Calcium-Binding Proteins; D002478:Cells, Cultured; D005089:Exocytosis / Q000187:drug effects / Q000276:immunology*; D015471:Leukemia, Basophilic, Acute; D008407:Mast Cells / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D013347:Subcellular Fractions / Q000276:immunology / Q000378:metabolism; D050863:Synaptotagmin I; D050857:Synaptotagmins; D014162:Transfection / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D002135:Calcium-Binding Proteins; D008562:Membrane Glycoproteins; D009419:Nerve Tissue Proteins; D050863:Synaptotagmin I; C494311:Syt1 protein, mouse; C494312:Syt1 protein, rat; D050857:Synaptotagmins; D000001:Calcimycin; D002118:Calcium