Non-isotopic in situ hybridization of HPV types in cervical intraepithelial lesions in patients with AIDS.
Human papilloma viruses (HPVs), particularly types 16 and 18 have a key role in the development of preneoplastic and neoplastic lesions of the uterine cervix. We studied, by non isotopic in situ hybridization using probes to HPV 6, 11, 16 and 18, cervical biopsies from AIDS patients with condilomata or cervical intraepithelial neoplasia. There were 32 biopsies which showed low-grade cervical intraepithelial neoplasia (Lo-CIN); 5 biopsies showed high-grade cervical intraepithelial neoplasia (Hi-CIN). Of 32 Lo-CIN biopsies, 18 (56.3%) were positive for HPV; 7 for HPV 6 and/or 11 (21.9%), 11 for HPV 16 and/or 18 (34.4%) and one for HPV 6 and 18. Of 5 Hi-CIN biopsies 3 were positive for HPV: one for HPV 6 and 2 for HPV 16 or 18. The total positivity was 56.8% (21/37). This result was similar to those obtained by various other authors studying the general population.
Calore
E E
EE
Department of Pathology, Faculty of Medicine, University of São Paulo, Brazil. calore@uol.com.br
Calore
N M
NM
Cavaliere
M J
MJ
D000163:Acquired Immunodeficiency Syndrome / Q000150:complications*; D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000821:virology*
D004279:DNA, Viral