Physical mapping of the linear plasmid pSLA2-L and localization of the eryAI and actI homologs.
The 200-kb linear plasmid pSLA2-L was suggested to be involved in the production of lankamycin and lankacidin in Streptomyces rochei 7434AN4. In this study, we have constructed a physical map for 23 PstI fragments of pSLA2-L, the sum of which was 206 kb. Detailed restriction maps for both ends of pSLA2-L revealed the presence of terminal inverted repeats, the size of which was found to be 2.1 kb by cloning and sequencing of the end-points. Hybridization experiments using two polyketide biosynthetic genes, eryAI and actI, located their homologous regions on PstI fragments A and I, respectively.
Kinashi
H
H
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan. kinashi@ipc.hiroshima-u.ac.jp
Fujii
S
S
Hatani
A
A
Kurokawa
T
T
Shinkawa
H
H
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D004917:Erythromycin / Q000031:analogs & derivatives / Q000096:biosynthesis; D006801:Humans; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D010957:Plasmids / Q000032:analysis*; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*
D000900:Anti-Bacterial Agents; D004917:Erythromycin; C100149:kujimycin B