Rat erythrocyte susceptibility to lipid peroxidation after chronic ethanol intake.
Erythrocytes, from 2- and 7-month-old rats chronically fed with a liquid diet containing ethanol, were analyzed for their susceptibility to lipid peroxidation estimated as thiobarbituric acid reactive substance (TBARS) products. In spite of a lower alcohol consumption, adult rats were the more affected by the ethanol treatment. Erythrocyte membranes prepared from alcoholic animals were more sensitive to lipid peroxidation than those prepared from control rats. In both age groups lipid analysis revealed similar changes: 1) an increased cholesterol/phospholipid molar ratio mainly derived from a higher content of cholesterol that accounts for the lower membrane fluidity and the higher tolerance to the disordering effect exerted by ethanol in vitro; 2) an increase of phospholipid unsaturated fatty acids, especially C20:4; 3) a modification of the phospholipid pattern, characterized in the ethanol group by an increase of PE and decrease of PE and decrease of PC levels; moreover, significant increases of the anionic phospholipids were detected in the adult group.
Lindi
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C
Institute of General Physiology and Biochemistry, Faculty of Pharmacy, University of Milan, Italy. lindi@imiucca.csi.unimi.it
Montorfano
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Marciani
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P
D000818:Animals; D016718:Arachidonic Acid / Q000097:blood; D002784:Cholesterol / Q000097:blood; D004910:Erythrocyte Membrane / Q000187:drug effects* / Q000378:metabolism; D000431:Ethanol / Q000008:administration & dosage* / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000097:blood; D019301:Oleic Acid / Q000097:blood; D019308:Palmitic Acid / Q000097:blood; D010743:Phospholipids / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D008563:Membrane Lipids; D010743:Phospholipids; D017392:Thiobarbituric Acid Reactive Substances; D016718:Arachidonic Acid; D019301:Oleic Acid; D019308:Palmitic Acid; D000431:Ethanol; D002784:Cholesterol