Immunomodulating activities of a natural alpha1-acid glycoprotein and its carbohydrate chains attached to the protein-free polymer.
Immunomodulating effects of a neoglycoconjugate created on the basis of alpha1-acid glycoprotein (AGP) carbohydrate chains and synthetic protein-free carrier have been investigated. It was demonstrated that this pseudo-AGP suppressed PHA- or anti-CD3 antibody-induced lymphocyte proliferation in a dose-dependent manner. Pseudo-AGP revealed a similar antiproliferative effect as the natural AGP samples. Stimulation of the LPS-induced proinflammatory cytokine production by mononuclear cells treated with both natural and pseudo-AGP has been also demonstrated. These data show that carbohydrate chains of AGP play a crucial role in the studied biological effect realization.
Pukhalsky
A L
AL
Research Centre for Medical Genetics, Moscow, Russia.
Shyian
S D
SD
Kalashnikova
E A
EA
Shmarina
G V
GV
Pukhalskaya
D A
DA
Bovin
N V
NV
D002241:Carbohydrates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D009961:Orosomucoid / Q000494:pharmacology*; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D002241:Carbohydrates; D015850:Interleukin-6; D009961:Orosomucoid; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha