Growth properties of HSIVnef: HIV-1 containing the nef gene from pathogenic molecular clone SIVmac239.
To elucidate the function of nef, we constructed infectious chimeric clones between human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV) of macaques, by deleting a part of the nef sequence from the HIV-1 genome and inserting the entire nef gene from the pathogenic molecular clone, SIVmac239. We have named this construct HSIVnef. While there was no difference in the replication kinetics of HSIVnef+ and HSIVnef- in transformed cell lines, there was a profound difference in the replication of these virus in primary cells. These data indicated that the SIV nef gene could complement the functions of the HIV-1 nef gene in the context of the HIV-1 genome, providing further evidence that results observed in the macaque model are relevant to HIV infections in man. HSIVnef is a useful model for the study of the nef gene.
Yoon
K
K
Institute for Molecular Biology and Genetics, Seoul National University, South Korea.
Kestler
H W
HW
Kim
S
S
D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D004279:DNA, Viral; D015693:Gene Products, nef / Q000235:genetics*; D015497:HIV-1 / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008251:Macaca; D008969:Molecular Sequence Data; D015302:Simian Immunodeficiency Virus / Q000235:genetics*; D054311:nef Gene Products, Human Immunodeficiency Virus
D004279:DNA, Viral; D015693:Gene Products, nef; D054311:nef Gene Products, Human Immunodeficiency Virus