The truncated form of the Epstein-Barr virus LMP-1 is dispensable or complimentable by the full-length form in virus infection and replication.
The Epstein-Barr virus (EBV) latent membrane protein-1 (LMP-1) gene of the Akata virus strain was cloned, and its nucleotide sequence was determined. Compared with the B95-8 strain, the translation initiation codon for the truncated LMP-1 gene, which is expressed in the lytic cycle, was lost. Immunoblotting showed that Akata EBV produces no truncated LMP-1 protein in any state and that the full-length LMP-1 protein is expressed at a significant level during lytic infection. The results suggest that the truncated LMP-1 protein is dispensable for EBV infection and replication or that the full-length form can "functionally" complement the truncated form if the truncated form has a function.
Torii
T
T
Cancer Institute, Hokkaido University School of Medicine, Sapporo, 060-8638, Japan.
Konishi
K
K
Sample
J
J
Takada
K
K
D001483:Base Sequence; D018387:Codon, Initiator; D004279:DNA, Viral / Q000378:metabolism; D015245:Deoxyribonuclease BamHI / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000472:pathogenicity* / Q000502:physiology*; D006801:Humans; D007163:Immunosorbent Techniques; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured; D014763:Viral Matrix Proteins / Q000737:chemistry / Q000235:genetics*; D014779:Virus Replication
D018387:Codon, Initiator; D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins; D015245:Deoxyribonuclease BamHI