Identification of three differentially expressed hydrophobins in Pleurotus ostreatus (oyster mushroom).
Three proteins with characteristic features of class I hydrophobins, designated POH1, POH2 and POH3, were isolated from the basidiomycete Pleurotus ostreatus. Based on N-terminal sequence analyses, their cDNAs were isolated using RT-PCR; the cDNAs and corresponding genes were sequenced and their regulation studied. POH1 is expressed in the fruiting bodies but not in vegetative mycelium. The regulation of POH2 and POH3 is tightly correlated. Both genes are switched off in the fruiting bodies but abundantly expressed in the vegetative mycelium of both monokaryon and dikaryon. POH2 and POH3 were isolated from the culture medium and from aerial hyphae. Co-purified POH2 and POH3 assembled in vitro into a protein membrane with a typical rodlet pattern as found previously with other hydrophobins. Similar structures were detected on the surface of aerial hyphae.
Asgeirsddttir
Sigridur A
SA
Molecular Plant Biology Laboratory, BiomolecuIar Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30,9751 NN Haren, The Netherlands.
de Vries
Onno M H
OMH
Molecular Plant Biology Laboratory, BiomolecuIar Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30,9751 NN Haren, The Netherlands.
Wessels
Joseph G H
JGH
Molecular Plant Biology Laboratory, BiomolecuIar Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30,9751 NN Haren, The Netherlands.
D000595:Amino Acid Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D003470:Culture Media; D018076:DNA, Complementary / Q000032:analysis; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D015151:Immunoblotting; D008969:Molecular Sequence Data; D020076:Pleurotus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012331:RNA, Fungal / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
D003470:Culture Media; D018076:DNA, Complementary; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal