Changes in interferon-regulatory factor-1 mRNA levels after transient ischemia in rat brain.
To evaluate whether the interferon system in the brain is activated by a severe form of metabolic stress, and to elucidate the possible mechanism underlying this activation, changes in the interferon regulatory factor-1 (irf-1) mRNA levels were evaluated after transient cerebral ischemia, and after exposure of primary neuronal cells to experimental conditions resulting in a depletion of ER calcium stores. Following 30 min ischemia and 2 h recovery, irf-1 mRNA levels rose significantly and stayed high for up to 24 h of recovery. Irf-1 mRNA levels were also significantly increased in neurons in vitro after exposing cells to conditions resulting in ER calcium store depletion with or without a parallel increase in cytoplasmic calcium activity. It is concluded that transient cerebral ischemia induces activation of the interferon system and that disturbances of ER calcium homeostasis may play a role in this process.
Paschen
W
W
Department of Experimental Neurology, Max-Planck-Institute for Neurological Research, Köln, Germany.
Gissel
C
C
Althausen
S
S
Doutheil
J
J
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism; D002560:Cerebrovascular Circulation; D002614:Chelating Agents / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004533:Egtazic Acid / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000502:physiology; D006624:Hippocampus / Q000098:blood supply / Q000737:chemistry / Q000378:metabolism; D050856:Interferon Regulatory Factor-1; D007372:Interferons / Q000378:metabolism; D002546:Ischemic Attack, Transient / Q000378:metabolism*; D008297:Male; D010750:Phosphoproteins / Q000235:genetics*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D019284:Thapsigargin / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics
D002614:Chelating Agents; D004268:DNA-Binding Proteins; D004791:Enzyme Inhibitors; D050856:Interferon Regulatory Factor-1; C494259:Irf1 protein, rat; D010750:Phosphoproteins; D012333:RNA, Messenger; D014157:Transcription Factors; D004533:Egtazic Acid; D019284:Thapsigargin; D007372:Interferons; D002118:Calcium