[HLA class II typing: determination of the allelic subtypes of HLA-DR*13 and DRB1*11 using PCR-SSP].
In this study, we reported the HLA-class II genotyping by PCR-SSP (PCR-Sequence Specific Primers). This method was used to subtype the HLA-DRB1*13 and 11 alleles in Dielmo population. The most frequent DRB1*11 subtypes were 1101, 1102 et 1104; the frequency of 1102 was the highest (18.5%). The DRB1*13 subtypes were more polymorphic in the population and DRB*1304 was the most common subtype (15%). There were significant differences between the ethnic groups for some subtypes of DRB1*11 and 13 alleles.
Dieye
A
A
Unité d'Immunologie, Institut Pasteur de Dakar, Sénégal.
Diaw
M L
ML
Roussilhon
C
C
Sarthou
J L
JL
D000483:Alleles; D017931:DNA Primers; D005787:Gene Frequency; D005838:Genotype; D006684:HLA-DR Antigens / Q000032:analysis / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012675:Senegal
D017931:DNA Primers; D006684:HLA-DR Antigens
Typage HLA classe II: détermination des sous-types des allèles HLA-DRB1*13 et DRB1*11 par PCR-SSP.