title: Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning.
abstract: Two members of a placental alkaline phosphatase (PLAP) family, PLAP and PLAP-like or germ cell alkaline phosphatase, are aberrantly expressed in tumors of ecotropic origin. To characterize alkaline phosphatase induced in seminoma, alkaline phosphatase cDNA clones were isolated from a cDNA library constructed from seminoma cells and characterized by nucleotide sequence determination. Thus, isolated cDNA clones were classified into two types, germ cell alkaline phosphatase (PLAP-like) and liver/bone/kidney-type alkaline phosphatase (L/B/K AP). These results suggest that other than the PLAP family members, the expression of L/B/K AP is enhanced in seminoma and can serve as a tumor marker in seminoma.
authors.affiliation: Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Shigenari
authors.forename: A
authors.initials: A
authors.lastname: Ando
authors.forename: T
authors.initials: T
authors.lastname: Baba
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuoka
authors.forename: H
authors.initials: H
authors.lastname: Inoko
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D018239:Seminoma / Q000201:enzymology*; D013736:Testicular Neoplasms / Q000201:enzymology*
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D000469:Alkaline Phosphatase