Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2.
The effects of chitin and chitosan on the release of arachidonic acid products were investigated in this study. Supernatants of canine polymorphonuclear cell (PMN) suspensions incubated with chitin and chitosan contained a leukotriene B4 (LTB4) concentration high enough to induce canine PMN migration in vitro. The supernatants also contained the same concentration of prostaglandin E2 (PGE2) as that normally found in the peripheral blood of dogs. Intraperitoneal administration of chitosan to dogs induced peritoneal exudative fluid (PEF), but chitin did not. The PEF contained numerous PMNs and macrophages. The supernatant of PEF contained both heat-stable and heat-labile chemotactic factors for canine PMNs. It also contained enough LTB4 to attract the canine PMNs in vitro.
Usami
Y
Y
Department of Veterinary Surgery, Tottori University, Japan.
Okamoto
Y
Y
Takayama
T
T
Shigemasa
Y
Y
Minami
S
S
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D001792:Blood Platelets / Q000166:cytology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects* / Q000502:physiology; D002686:Chitin / Q000031:analogs & derivatives* / Q000494:pharmacology*; D048271:Chitosan; D015232:Dinoprostone / Q000097:blood*; D004285:Dogs; D004912:Erythrocytes / Q000166:cytology; D005122:Exudates and Transudates / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D005260:Female; D066298:In Vitro Techniques; D007962:Leukocytes / Q000166:cytology; D007975:Leukotriene B4 / Q000097:blood*; D008297:Male; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology
D001672:Biocompatible Materials; D002686:Chitin; D007975:Leukotriene B4; D048271:Chitosan; D015232:Dinoprostone