A developmental profile of the effects of ethanol on the levels of chick brain phospholipids.
The effects of embryonic exposure to ethanol on brain phospholipid levels were studied by injecting various concentrations of ethanol, ranging from 0 to 149 microns kg-1 egg, into fertile chicken eggs at 0 days of incubation. At 7, 9, 11, 15 and 18 days of incubation, brains were collected and the levels of total phospholipids and various phospholipid classes were measured. Although embryonic exposure to ethanol failed to influence total phospholipid levels, ethanol-induced changes in the levels of individual phospholipid classes were observed. Ethanol-induced increases in the levels of phosphatidylethanolamine (PE) and phosphatidylserine (PS) and ethanol-induced decreases in the levels of phosphatidylcholine (PC) were observed at 9, 11, 15 and 18 days of incubation. Ethanol-induced decreases in brain sphingomyelin (SP) levels were observed at 7 and 18 days of development. These ethanol-induced changes in brain phospholipid levels preceded detectable alcohol dehydrogenase (ADH) activities in both brain and liver.
Miller
R R
RR
Biology Department, Hillsdale College, MI 49242-1037, USA. b.miller@ac.hillsdale.edu
Touney
E J
EJ
Vandivier
W J
WJ
Raymond
F J
FJ
D000079:Acetaldehyde / Q000378:metabolism / Q000633:toxicity; D000426:Alcohol Dehydrogenase / Q000096:biosynthesis / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000196:embryology / Q000502:physiology*; D002492:Central Nervous System Depressants / Q000378:metabolism / Q000633:toxicity*; D002642:Chick Embryo; D000431:Ethanol / Q000378:metabolism / Q000633:toxicity*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D010743:Phospholipids / Q000378:metabolism*
D002492:Central Nervous System Depressants; D010743:Phospholipids; D000431:Ethanol; D000426:Alcohol Dehydrogenase; D000079:Acetaldehyde