The lymnaea cardioexcitatory peptide (LyCEP) receptor: a G-protein-coupled receptor for a novel member of the RFamide neuropeptide family.
A novel G-protein-coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106, Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaea brain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In the Lymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEP in vitro. These data confirm that LyCEP is an RFamide ligand for GRL106.
Tensen
C P
CP
Department of Biochemistry and Molecular Biology, Research Institute Neurosciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands.
Cox
K J
KJ
Smit
A B
AB
van der Schors
R C
RC
Meyerhof
W
W
Richter
D
D
Planta
R J
RJ
Hermann
P M
PM
van Minnen
J
J
Geraerts
W P
WP
Knol
J C
JC
Burke
J F
JF
Vreugdenhil
E
E
van Heerikhuizen
H
H
D000200:Action Potentials / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000294:innervation; D008195:Lymnaea / Q000235:genetics*; D008969:Molecular Sequence Data; D009412:Nerve Fibers / Q000737:chemistry / Q000378:metabolism; D009420:Nervous System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014981:Xenopus
D015342:DNA Probes; D018076:DNA, Complementary; C115978:G protein-coupled receptor LyCEP; D006023:Glycoproteins; D009479:Neuropeptides; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; C058047:arginylphenylalaninamide; D019204:GTP-Binding Proteins