title: Chicken lutropin acts like follitropin in rat ovarian follitropin receptor: an isoelectric focusing study.
abstract: This study investigates whether chicken lutropin (LH) specifically binds to rat ovarian follitropin (FSH) receptor and exerts FSH-like bioactivity. Glycoprotein fraction, prepared from the chicken anterior pituitary gland, was fractionated using isoelectric focusing within a pH range of 3.5-11. Analysis of the focused fractions, by a radioreceptor assay (RRA) specific for FSH in rats using rat ovarian homogenate as receptor source, and 125I-labeled rat FSH as radioligand, detected a large component having an isoelectric point of 10.25. This focusing profile obtained by RRA was quite similar to that obtained by a specific radioimmunoassay (RIA) for chicken LH, but clearly different from that obtained by a specific RIA for chicken FSH, indicating this RRA specifically recognizes chicken LH. Chicken LH fraction prepared from the electrofocused material was used for further studies. The chicken LH preparation was three times more potent than rat FSH in the RRA in displacing the radioligand bound to rat ovarian receptor, while chicken LH facilitated an 8-fold less production of estradiol in dispersed rat granulosa cells than rat FSH. These results suggest that chicken LH acts like rat FSH in rat ovarian FSH receptor, but receptor-binding activity is much higher than biological activity.
authors.affiliation: Department of Animal Science and Technology, Faculty of Agriculture, Gifu University, Japan. aiwa@cc.gifu-u.ac.jp
authors.forename: A
authors.initials: A
authors.lastname: Iwasawa
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: Y
authors.initials: Y
authors.lastname: Fukuhara
authors.forename: M
authors.initials: M
authors.lastname: Kawashima
authors.forename: K
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: M
authors.initials: M
authors.lastname: Kamiyoshi
mesh_terms: D000818:Animals; D002645:Chickens; D002845:Chromatography; D005260:Female; D005640:Follicle Stimulating Hormone / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D007457:Iodine Radioisotopes; D007525:Isoelectric Focusing; D007986:Luteinizing Hormone / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D008297:Male; D010053:Ovary / Q000378:metabolism / Q000648:ultrastructure*; D010903:Pituitary Gland, Anterior / Q000737:chemistry; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D011962:Receptors, FSH / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D012685:Sepharose / Q000031:analogs & derivatives; D013045:Species Specificity
chemical_list: D007457:Iodine Radioisotopes; D011962:Receptors, FSH; C028326:concanavalin A-sepharose; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D012685:Sepharose