Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry.
A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-microl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH2PO4 (pH 7.4) buffer and from 100 microl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats.
Kakkar
T
T
Department of Pharmacy Practice and Science, and the Center for Toxicology, College of Pharmacy, University of Arizona, Tucson 85721, USA.
Mayersohn
M
M
D000818:Animals; D001826:Body Fluids / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012459:Salicylates / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D020156:Salicylic Acid / Q000032:analysis* / Q000097:blood; D012680:Sensitivity and Specificity
D012459:Salicylates; C041835:ethyl salicylate; C033069:methyl salicylate; D020156:Salicylic Acid