Performance of six cell lines in the suspension-infection test used for the detection of herpes simplex virus.
Six cell lines were assessed in the suspension-infection (SI) test for suitability for the rapid culture of herpes simplex virus (HSV). For the SI test, the specimen was combined in growth medium with trypsinized, suspended culture cells before allowing the cells to settle into a monolayer growth pattern. The cells tested in the SI assay were MV1-Lu, vero C1008, BSC-1, RD, and MRC-9 cells. Fifty clinical specimens composed of 7 HSV-1-positive samples, 9 HSV-2-positive samples, 12 positive for HSV (not typed), and 22 HSV-negative samples were tested. For the detection of HSV in clinical specimens, MV1Lu cells were most sensitive and demonstrated large, darkly stained foci of virus infection.
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Department of Microbiology, Brigham Young University, Provo, UT 84602, USA.
Johnson
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D000818:Animals; D002460:Cell Line / Q000821:virology; D003588:Cytopathogenic Effect, Viral; D005835:Genitalia / Q000821:virology; D006558:Herpes Genitalis / Q000175:diagnosis / Q000821:virology; D006561:Herpes Simplex / Q000175:diagnosis / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000254:growth & development / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000254:growth & development / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques; D009055:Mouth / Q000821:virology; D012680:Sensitivity and Specificity; D012703:Serotyping; D018139:Simplexvirus / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification*; D013283:Stomatitis, Herpetic / Q000175:diagnosis / Q000821:virology; D014773:Virology / Q000379:methods*