title: Vulvar squamous carcinoma: prognostic role of DNA content.
abstract: DNA nuclear content was evaluated on 46 paraffin-embedded blocks of vulvar cancer by means of flow cytometry. Fifteen of 44 evaluable samples (34%) were found to have an aneuploid DNA pattern. Aneuplody showed a statistically significant relationship with tumor grading (P = 0.0004) and defined a subset of patients who have pathological characteristics of high risk, such as advanced stage of disease, deepest stromal invasion, or positive inguinal nodes. However, when a multivariate regression model was used, ploidy status did not have any prognostic role, while FIGO staging system was confirmed to be the strongest independent prognostic factor (P = 0.009). The integration of a panel of cell kinetic parameters with traditional features is worth investigating on a larger population.
authors.affiliation: Department of Gynecologic Oncology, Regina Elena Cancer Institute of Rome, Rome, 00162, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mariani
authors.forename: L
authors.initials: L
authors.lastname: Conti
authors.forename: G
authors.initials: G
authors.lastname: Atlante
authors.forename: F
authors.initials: F
authors.lastname: Sciarretta
authors.forename: M
authors.initials: M
authors.lastname: Pozzi
authors.forename: M
authors.initials: M
authors.lastname: Vercillo
authors.forename: V
authors.initials: V
authors.lastname: Francavilla
authors.forename: L
authors.initials: L
authors.lastname: De Franceschi
authors.forename: S
authors.initials: S
authors.lastname: Rossi
authors.forename: R
authors.initials: R
authors.lastname: Sindico
authors.forename: M
authors.initials: M
authors.lastname: Atlante
authors.forename: G M
authors.initials: GM
authors.lastname: Gandolfo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000401:mortality* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008207:Lymphatic Metastasis; D008875:Middle Aged; D009367:Neoplasm Staging; D011003:Ploidies; D011379:Prognosis; D012189:Retrospective Studies; D014846:Vulvar Neoplasms / Q000401:mortality* / Q000473:pathology
chemical_list: D004273:DNA, Neoplasm