Genetic variation of mitochondrial cytochrome b genes among the subspecies of koala, Phascolarctos cinereus.
A conserved DNA region among the subspecies of koala, of mitochondrial cytochrome b gene, was employed to analyze the genetic variation among 3 available subspecies of koala. This conserved sequence, 307 bp DNA, was sequenced using polymerase chain reaction and direct DNA sequencing technique. Substitutions in the nucleotide sequences were observed, with which koalas can be divided into 3 DNA haplotypes subspecies, but the molecular data provided inconsistency with current classification of the 3 subspecies of koala.
Takami
K
K
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Yoshida
M
M
Yamamoto
Y
Y
Harada
M
M
Furuyama
J
J
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D003573:Cytochrome b Group / Q000235:genetics*; D004247:DNA / Q000737:chemistry; D014644:Genetic Variation; D008394:Marsupialia / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D003573:Cytochrome b Group; D004247:DNA