Expression and recovery of functional G-protein-coupled receptors using baculovirus expression systems.
Baculovirus expression systems have been used for more than ten years as the tool of choice to over-express G-protein-coupled receptors. Although this expression system has also been used to study the signaling mechanisms of the receptors at the cellular level, it was found to be a most useful method to produce large quantities of receptors for biochemical and biophysical studies. Methods that allow easy and selective recovery of properly folded and mature receptors in viral particles open new perspectives for such applications.
Bouvier
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M
Department of Biochemistry, Université de Montréal, Qc., Canada. bouvier@bch.umontreal.ca
Ménard
L
L
Dennis
M
M
Marullo
S
S
D000818:Animals; D001709:Biotechnology; D002460:Cell Line; D019204:GTP-Binding Proteins / Q000378:metabolism*; D015870:Gene Expression; D017924:Nucleopolyhedroviruses / Q000235:genetics*; D011499:Protein Processing, Post-Translational; D011869:Radioligand Assay; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018411:Spodoptera
D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D019204:GTP-Binding Proteins