Monoclonal antibodies against vascular endothelial antigens expressed in normal human brain.
A panel of monoclonal antibodies reactive with human-brain vessels was raised by immunizing BALB/c mice with homogenate of whole human brain, obtained from temporal lobectomies. Hybridoma supernates were screened by immunohistochemical methods on frozen sections of human brain, liver and spleen and 16 clones were isolated. The pattern of immunoreactivity varied with respect to the type of brain blood vessels predominantly labelled and to tissue specificity. Some antibodies cross-reacted with cow or squirrel monkey forebrain microvessels with an intensity equal to that shown by human brain. The immunoreactivity patterns reflected antigenic heterogeneity among different subsets of vascular endothelial cells in human brain.
Ibiwoye
M O
MO
Molecular Biology and Immunology Division, Liverpool School of Tropical Medicine, UK.
Broome
J C
JC
Rossi
M L
ML
Eldridge
P
P
Charters
P
P
Hommel
M
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D000918:Antibody Specificity; D000943:Antigens, Differentiation / Q000096:biosynthesis / Q000276:immunology* / Q000378:metabolism; D001921:Brain / Q000098:blood supply / Q000276:immunology*; D002417:Cattle; D004730:Endothelium, Vascular / Q000276:immunology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000098:blood supply / Q000276:immunology; D008099:Liver / Q000098:blood supply / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D009928:Organ Specificity / Q000276:immunology; D051381:Rats; D012453:Saimiri; D013154:Spleen / Q000098:blood supply / Q000276:immunology
D000911:Antibodies, Monoclonal; D000943:Antigens, Differentiation