title: The lymnaea cardioexcitatory peptide (LyCEP) receptor: a G-protein-coupled receptor for a novel member of the RFamide neuropeptide family.
abstract: A novel G-protein-coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106, Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaea brain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In the Lymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEP in vitro. These data confirm that LyCEP is an RFamide ligand for GRL106.
authors.affiliation: Department of Biochemistry and Molecular Biology, Research Institute Neurosciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands.
authors.forename: C P
authors.initials: CP
authors.lastname: Tensen
authors.forename: K J
authors.initials: KJ
authors.lastname: Cox
authors.forename: A B
authors.initials: AB
authors.lastname: Smit
authors.forename: R C
authors.initials: RC
authors.lastname: van der Schors
authors.forename: W
authors.initials: W
authors.lastname: Meyerhof
authors.forename: D
authors.initials: D
authors.lastname: Richter
authors.forename: R J
authors.initials: RJ
authors.lastname: Planta
authors.forename: P M
authors.initials: PM
authors.lastname: Hermann
authors.forename: J
authors.initials: J
authors.lastname: van Minnen
authors.forename: W P
authors.initials: WP
authors.lastname: Geraerts
authors.forename: J C
authors.initials: JC
authors.lastname: Knol
authors.forename: J F
authors.initials: JF
authors.lastname: Burke
authors.forename: E
authors.initials: E
authors.lastname: Vreugdenhil
authors.forename: H
authors.initials: H
authors.lastname: van Heerikhuizen
mesh_terms: D000200:Action Potentials / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D015342:DNA Probes; D018076:DNA, Complementary; D004594:Electrophysiology; D019204:GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism; D006321:Heart / Q000294:innervation; D008195:Lymnaea / Q000235:genetics*; D008969:Molecular Sequence Data; D009412:Nerve Fibers / Q000737:chemistry / Q000378:metabolism; D009420:Nervous System / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D009479:Neuropeptides / Q000235:genetics* / Q000378:metabolism; D009865:Oocytes / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis; D018013:Receptors, Neuropeptide / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D014981:Xenopus
chemical_list: D015342:DNA Probes; D018076:DNA, Complementary; C115978:G protein-coupled receptor LyCEP; D006023:Glycoproteins; D009479:Neuropeptides; D012333:RNA, Messenger; D018013:Receptors, Neuropeptide; C058047:arginylphenylalaninamide; D019204:GTP-Binding Proteins