Reconstitution of UV-damaged DNA into chromatin using Xenopus oocyte extracts.
Chromatin was reconstituted in vitro using Xenopus oocyte extracts and plasmid DNA containing UV radiation-induced damage. Damaged DNA was assembled into minichromosomes with an efficiency similar to that of control, non-irradiated DNA. Oocyte extracts were competent to carry out DNA repair, which was elicited by nicking damaged templates followed by DNA synthesis during chromatin assembly. Newly synthesized DNA was efficiently reconstituted into nucleosomes.
Widłak
P
P
Department of Experimental and Clinical Radiobiology, Institute of Oncology, Gliwice, Poland. widlak@onkol.instonko.gliwice.pl
D000818:Animals; D002843:Chromatin; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair; D004261:DNA Replication; D005260:Female; D013698:Templates, Genetic; D014466:Ultraviolet Rays; D014981:Xenopus
D002843:Chromatin