Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.
In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L. rhamnosus Lc 1/3 was constructed based on the sequence. The primer pair was tested with 11 Lactobacillus species and 11 L. rhamnosus strains and was found to be strain specific. The nucleotide sequence of the specific RAPD marker was found to contain part of a protein encoding region which showed significant similarity to several transposases for insertion sequence elements of various bacteria, including other lactic acid bacterium species.
Tilsala-Timisjärvi
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Department of Biology, University of Oulu, Linnanmaa, FIN-90570 Oulu, Finland. atilsala@cc.oulu.fi
Alatossava
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D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers; D005819:Genetic Markers; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics*; D008969:Molecular Sequence Data; D019105:Random Amplified Polymorphic DNA Technique; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
D017931:DNA Primers; D005819:Genetic Markers