Factors affecting protein transfer into surfactant-isooctane solution: a case study of extraction behavior of chemically modified cytochrome c.
The extraction mechanism of proteins by surfactant molecules in an organic solvent has been investigated using a chemically modified protein. We conducted guanidylation on lysine residues of cytochrome c by replacing their amino groups with homoarginine to enhance the protein-surfactant interaction. Results have shown that guanidylated cytochrome c readily forms a hydrophobic complex with dioleyl phosphoric acid (DOLPA) through hydrogen bonding between the phosphate moiety and the guanidinium groups. Although improved protein-surfactant interaction activated the formation of a hydrophobic complex at the interface, it could not improve the protein transfer in isooctane. It has been established that the protein extraction mechanism using surfactant molecules is mainly governed by two processes: formation of an interfacial complex at the oil-water interface and the subsequent solubilization of the complex into the organic phase. In addition, a kinetic study demonstrated that guanidylation of lysine accelerated the initial extraction rate of cytochrome c. This fact implies that the protein transferability from aqueous phase into organic phase depends on the protein-surfactant interaction which can be modified by protein surface engineering.
Ono
T
T
Department of Chemical Science & Technology, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan.
Goto
M
M
D000818:Animals; D003574:Cytochrome c Group / Q000737:chemistry* / Q000302:isolation & purification*; D019791:Guanidine; D006709:Homoarginine; D006736:Horses; D006860:Hydrogen Bonding; D008239:Lysine; D009113:Muramidase / Q000737:chemistry / Q000302:isolation & purification*; D009206:Myocardium / Q000378:metabolism; D009795:Octanes; D009829:Oleic Acids; D012996:Solutions; D013501:Surface-Active Agents
D003574:Cytochrome c Group; D009795:Octanes; D009829:Oleic Acids; D012996:Solutions; D013501:Surface-Active Agents; D006709:Homoarginine; C010938:dioleoyl phosphate; D009113:Muramidase; D019791:Guanidine; D008239:Lysine; C045798:2,2,4-trimethylpentane