title: 13q14 deletions are not primary events in B-cell chronic lymphocytic leukemia: a study of 100 patients using fluorescence in situ hybridization.
abstract: Fluorescence in situ hybridization with a chromosome 12-specific alpha-centromeric probe and a 13q14 yeast artificial chromosome probe was performed on interphase cells from 100 patients with B-cell chronic lymphocytic leukemia. Thirty-one patients exhibited a 13q14 deletion. No correlation was found between 13q14 deletions and clinical stage, sex, or morphology. Sixteen patients had trisomy 12, including 6 (of 12) with an atypical morphology. Trisomy 12 and 13q14 abnormalities were detected concomitantly in three patients only. The analysis of patients with deletions clearly showed that in five cases a significant number of cells retained two signals with the yeast artificial chromosome probe, indicating a genetic heterogeneity among the leukemic population. Our data confirm that the 13q14 deletion is a frequent event, indicate that the concomitant occurrence of 13q14 deletion and trisomy 12 is rare but possible, and show that both abnormalities are secondary events in B-cell chronic lymphocytic leukemia.
authors.affiliation: Laboratory of Hematology, Laboratory of Molecular Genetics, and Department of Clinical Hematology, University Hospital, 44035 Nantes, Cedex, France.
authors.forename: H
authors.initials: H
authors.lastname: Avet-Loiseau
authors.forename: M C
authors.initials: MC
authors.lastname: Devilder
authors.forename: R
authors.initials: R
authors.lastname: Garand
authors.forename: I
authors.initials: I
authors.lastname: Bouyge
authors.forename: M J
authors.initials: MJ
authors.lastname: Rapp
authors.forename: N
authors.initials: N
authors.lastname: Milpied
authors.forename: J L
authors.initials: JL
authors.lastname: Harousseau
authors.forename: J P
authors.initials: JP
authors.lastname: Moisan
authors.forename: R
authors.initials: R
authors.lastname: Bataille
mesh_terms: D002872:Chromosome Deletion; D018244:Chromosomes, Artificial, Yeast; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics*; D015342:DNA Probes; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000473:pathology; D014314:Trisomy
chemical_list: D015342:DNA Probes; D005819:Genetic Markers