title: Expression and localization of brain ankyrin isoforms and related proteins during early developmental stages of rat nervous system.
abstract: Expression and localization of two isoforms of brain ankyrin, 440- and 220-kDa ankyrinB, were studied in the developing nervous system of the rat fetus. The 440-kDa ankyrinB appeared on as early as embryonic day 13, and its level increased progressively toward the day of birth, which was similar to the expression pattern of growth-associated protein (GAP)-43, a well-established axonal protein. On the other hand, 220-kDa ankyrinB was expressed at a low level but constitutively throughout the latter prenatal period and was a major isoform even before embryonic day 14. Whereas the localization of 440-kDa ankyrinB was essentially confined to the axons, judging from the similarity with that of GAP-43, 220-kDa ankyrinB showed a rather general distribution in neural tissue. The localization of L1, known as an ankyrinB-binding protein, was similar to that of 440-kDa ankyrinB in the brain tissue, whereas it was similar to that of 220-kDa ankyrinB in cultured neurons, suggesting that the interaction of L1 with brain ankyrins in neurons is affected by their environment.
authors.affiliation: Regional Environment Division, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Kunimoto
authors.forename: T
authors.initials: T
authors.lastname: Adachi
authors.forename: M
authors.initials: M
authors.lastname: Ishido
mesh_terms: D000818:Animals; D017487:Ankyrins / Q000378:metabolism*; D001921:Brain / Q000196:embryology* / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology / Q000378:metabolism; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000502:physiology; D007150:Immunohistochemistry; D007536:Isomerism; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution
chemical_list: D017487:Ankyrins; D002352:Carrier Proteins; D015815:Cell Adhesion Molecules