title: Caloric restriction in rhesus monkeys reduces low density lipoprotein interaction with arterial proteoglycans.
abstract: Caloric restriction (CR) has been shown to retard aging processes in many species. We investigated effects of CR on plasma low density lipoproteins (LDL), a major risk factor for the age-associated process of atherosclerosis. Studies emphasized effects of CR on LDL composition and their interaction with arterial proteoglycans (PG). Rhesus monkeys were fed a control diet (n=13) or subjected to CR (n=12 fed 30% less calories) for > 5 years. Plasma LDL cholesterol concentrations were similar for control and CR groups (82+/-8 vs 72+/-6 mg/dL, mean+/-SEM). LDL was isolated by ultracentrifugation and HPLC. LDL particles from CR animals had a lower molecular weight (2.9+/-0.1 vs 3.2+/-0.1 g/micromol, p < .05) and were depleted in triglyceride (249+/-16 vs 433+/-49 mol/particle, p < .005) and phospholipid (686+/-20 vs 837+/-33 mol/particle, p <.001). Lower PG binding was measured for LDL from CR animals (10.1+/-0.8 vs 15.6+/-1.1 microg LDL cholesterol/microg PG, p <.005). This was associated with the lower triglycerides (r=.76, p < .0005) and phospholipids (r=.48, p < .01). Thus, a dietary intervention which may retard aging inhibits a proposed mechanism of atherogenesis.
authors.affiliation: Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. iedwards@wfubmc.edu
authors.forename: I J
authors.initials: IJ
authors.lastname: Edwards
authors.forename: L L
authors.initials: LL
authors.lastname: Rudel
authors.forename: J G
authors.initials: JG
authors.lastname: Terry
authors.forename: J W
authors.initials: JW
authors.lastname: Kemnitz
authors.forename: R
authors.initials: R
authors.lastname: Weindruch
authors.forename: W T
authors.initials: WT
authors.lastname: Cefalu
mesh_terms: D000818:Animals; D001158:Arteries / Q000378:metabolism*; D004347:Drug Interactions; D002149:Energy Intake; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood / Q000737:chemistry / Q000502:physiology*; D008253:Macaca mulatta; D011509:Proteoglycans / Q000502:physiology*
chemical_list: D008055:Lipids; D008077:Lipoproteins, LDL; D011509:Proteoglycans