title: Prognostic value of amplification of c-erb-B2 in bladder carcinoma.
abstract: Amplification of c-erb-B2 is examined in patients with transitional cell bladder carcinoma. In a pilot study we found that the amplification correlated with high tumor grade. Tumor grade is a known prognostic factor. Therefore, we next examined the prognostic value of c-erb-B2 amplification in patients with >16 years of clinical follow-up. The gene copy number was determined, using semiquantitative PCR, in archival formalin-fixed tissues. Twenty-three percent (37/163 patients) showed the amplification. The amplification correlated with grade and stage. Moreover, we found that tumor grade (P < 0.001) and c-erb-B2 amplification (P < 0.001) showed prognostic information for survival. Patients with grade 3 tumors and concomitant c-erb-B2 amplification showed the worst prognosis. Multivariate analysis indicates that grade and c-erb-B2 amplification are independent prognostic factors.
authors.affiliation: Radiumhemmet, Department of Cancer Epidemiology, Division of Cytology, Karolinska Hospital, S-171 76 Stockholm, Sweden.
authors.forename: U
authors.initials: U
authors.lastname: Lönn
authors.forename: S
authors.initials: S
authors.lastname: Lönn
authors.forename: S
authors.initials: S
authors.lastname: Friberg
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
authors.forename: C
authors.initials: C
authors.lastname: Silfverswärd
authors.forename: B
authors.initials: B
authors.lastname: Stenkvist
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D002295:Carcinoma, Transitional Cell / Q000235:genetics* / Q000473:pathology; D005784:Gene Amplification; D018734:Genes, erbB-2 / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D008875:Middle Aged; D010865:Pilot Projects; D011379:Prognosis; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D005819:Genetic Markers