Characterisation of Escherichia coli O157:H7 (H-) derived from cattle using random amplification of polymorphic DNA analysis.
One hundred and two isolates of Escherichia coli O157:H7 derived from cattle were characterised by random amplification of polymorphic DNA (RAPD). Four different RAPD profiles were observed. Based on the combined results of RAPD typing and toxin genotyping, the isolates could be divided into six distinct groups.
Katsuda
K
K
Feed Safety Research Division, National Institute of Animal Health, Tsukuba, Ibaraki, Japan. katsuda@niah.affrc.go.jp
Sameshima
T
T
Akiba
M
M
Nakazawa
M
M
D000818:Animals; D002417:Cattle / Q000382:microbiology*; D003611:Dairy Products / Q000382:microbiology; D004196:Disease Outbreaks; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000635:transmission*; D019453:Escherichia coli O157 / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008460:Meat / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique / Q000662:veterinary*; D012703:Serotyping