title: Immunomodulating activities of a natural alpha1-acid glycoprotein and its carbohydrate chains attached to the protein-free polymer.
abstract: Immunomodulating effects of a neoglycoconjugate created on the basis of alpha1-acid glycoprotein (AGP) carbohydrate chains and synthetic protein-free carrier have been investigated. It was demonstrated that this pseudo-AGP suppressed PHA- or anti-CD3 antibody-induced lymphocyte proliferation in a dose-dependent manner. Pseudo-AGP revealed a similar antiproliferative effect as the natural AGP samples. Stimulation of the LPS-induced proinflammatory cytokine production by mononuclear cells treated with both natural and pseudo-AGP has been also demonstrated. These data show that carbohydrate chains of AGP play a crucial role in the studied biological effect realization.
authors.affiliation: Research Centre for Medical Genetics, Moscow, Russia.
authors.forename: A L
authors.initials: AL
authors.lastname: Pukhalsky
authors.forename: S D
authors.initials: SD
authors.lastname: Shyian
authors.forename: E A
authors.initials: EA
authors.lastname: Kalashnikova
authors.forename: G V
authors.initials: GV
authors.lastname: Shmarina
authors.forename: D A
authors.initials: DA
authors.lastname: Pukhalskaya
authors.forename: N V
authors.initials: NV
authors.lastname: Bovin
mesh_terms: D002241:Carbohydrates / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D009961:Orosomucoid / Q000494:pharmacology*; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
chemical_list: D002241:Carbohydrates; D015850:Interleukin-6; D009961:Orosomucoid; D011108:Polymers; D014409:Tumor Necrosis Factor-alpha