Thyroid stimulating hormone and selenium supply interact to regulate selenoenzyme gene expression in thyroid cells (FRTL-5) in culture.
In the absence of a sodium selenite supplement, FRTL-5 cells showed a reduced activity of cytosolic glutathione peroxidase (cGSH-Px), a marker of selenium status, indicating the cells were Se-deficient. Se-deficient cells showed a 65% reduction in cGSH-Px mRNA abundance but little change in abundance of either phospholipid hydroperoxide glutathione peroxidase or type 1 deiodinase (IDI) mRNA. In Se-replete cells increased thyroid stimulating hormone (TSH) caused a small decrease in IDI abundance but in Se-deficient cells TSH caused a large increase. The results indicate an interaction between TSH and Se status in the regulation of thyroid selenoenzyme synthesis.
Villette
S
S
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Bermano
G
G
Arthur
J R
JR
Hesketh
J E
JE
D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D003470:Culture Media; D003600:Cytosol / Q000201:enzymology; D004847:Epithelial Cells / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D005979:Glutathione Peroxidase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007453:Iodide Peroxidase / Q000235:genetics; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D012643:Selenium / Q000172:deficiency / Q000378:metabolism / Q000494:pharmacology*; D013961:Thyroid Gland / Q000166:cytology / Q000201:enzymology*; D013972:Thyrotropin / Q000494:pharmacology*
D003470:Culture Media; D012333:RNA, Messenger; D013972:Thyrotropin; D000080662:Phospholipid Hydroperoxide Glutathione Peroxidase; D007453:Iodide Peroxidase; D005979:Glutathione Peroxidase; D012643:Selenium