Identification of a novel mechanism of regulation of the adherens junction by E1A, Rac1, and cortical actin filaments that contributes to tumor progression.
Transformation progression toward more malignant behavior often results from a loss of epithelial cell behavior, especially cell-cell adhesion. E1A cooperates with ras to transform primary epithelial cells such that they maintain epithelial cell differentiation, including the proper localization of adherens junctions (AJs). Second exon mutants of E1A 12S cooperate with ras to produce a more aggressively transformed phenotype, termed hypertransformation, that includes the loss of adhesion. Such hypertransformation can also be achieved by the addition of activated Rac1 to cells expressing wild-type E1A and ras, suggesting that actin reorganization may be important for the hypertransformed phenotype. Primary epithelial cells expressing hypertransforming mutants of E1A or V12Rac1 exhibit the loss of cortical actin filaments. In these cells, AJ complexes do not incorporate alpha-catenin, fail to associate with the cytoskeleton, and fail to localize to the plasma membrane, resulting in the destabilization of the AJ components and a loss of function. Loss of these epithelial cell characteristics predisposes these cells to a more malignant phenotype due to the loss of cell-cell adhesion. Taken together, these results suggest a novel mechanism of regulation of AJ function in tumor progression that involves the correct targeting of the AJ components, and this is affected by the status of cortical actin, which can be differentially affected by E1A or Rac1.
Fischer
R S
RS
Department of Microbiology & Immunology, University of Tennessee Health Science Center, Memphis 38163, USA.
Quinlan
M P
MP
D008841:Actin Cytoskeleton; D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000818:Animals; D015820:Cadherins / Q000096:biosynthesis; D002461:Cell Line, Transformed; D002462:Cell Membrane / Q000378:metabolism; D051356:Cortactin; D003598:Cytoskeletal Proteins / Q000378:metabolism; D003599:Cytoskeleton; D018450:Disease Progression; D005089:Exocytosis; D019204:GTP-Binding Proteins / Q000235:genetics*; D005786:Gene Expression Regulation; D011905:Genes, ras; D007365:Intercellular Junctions / Q000378:metabolism*; D007424:Intracellular Fluid; D008840:Microfilament Proteins / Q000235:genetics*; D010455:Peptides / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation; D051381:Rats; D011916:Rats, Inbred F344; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D020744:rac GTP-Binding Proteins
D017500:Adenovirus E1A Proteins; D015820:Cadherins; D051356:Cortactin; C495268:Ctnnb1 protein, rat; C495870:Cttn protein, rat; D003598:Cytoskeletal Proteins; D008840:Microfilament Proteins; D010455:Peptides; D015534:Trans-Activators; D051178:alpha Catenin; D051176:beta Catenin; D019204:GTP-Binding Proteins; D020744:rac GTP-Binding Proteins