title: Utility of green fluorescent nucleic acid dyes and aluminum oxide membrane filters for rapid epifluorescence enumeration of soil and sediment bacteria.
abstract: High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4',6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.
authors.affiliation: GBF-National Research Center of Biotechnology, AG Microbial Ecology, D-38124 Braunschweig, Germany. mgw@gbf.de
authors.forename: M G
authors.initials: MG
authors.lastname: Weinbauer
authors.forename: C
authors.initials: C
authors.lastname: Beckmann
authors.forename: M G
authors.initials: MG
authors.lastname: Höfle
mesh_terms: D000537:Aluminum Oxide; D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D004926:Escherichia coli / Q000145:classification / Q000302:isolation & purification; D005456:Fluorescent Dyes; D005858:Germany; D007211:Indoles; D009930:Organic Chemicals; D011549:Pseudomonas / Q000145:classification / Q000302:isolation & purification; D012988:Soil Microbiology; D014871:Water Microbiology
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D009930:Organic Chemicals; C098798:SYBR Green II; C007293:DAPI; D000537:Aluminum Oxide