title: Adenomatous polyposis coli protein is expressed in alternate stages of the ameloblast life cycle.
abstract: Mutations of the adenomatous polyposis coli gene protein (APC) are associated with familial polyposis and also sporadic colon adenomas, both preconditions to cancer formation. Some familial polyposis patients also develop Gardner's syndrome, a condition characterized by supernumerary teeth, mandibular osteomas, and other maladies. We investigated participation of APC in normal tooth development. Using a monoclonal antibody to study APC expression in the forming rat incisor, we found no APC staining in differentiating ameloblasts, then strong staining in secreting ameloblasts and stratum intermedium cells, followed by cells in the transition stage which did not stain. Intense APC staining resumes in maturation-stage ameloblasts and proximal papillary cells. APC staining disappears again in reduced ameloblasts at the conclusion of amelogenesis. APC staining was not seen in any other odontogenic cells. We report a unique system in which APC expression is upregulated and downregulated twice during the normal life cycle of ameloblasts. APC, therefore, is important in the normal maturation of both colonic epithelium and odontogenic epithelium. At this point, we cannot rule out any of the known functions of APC, which include: modulation of cell adhesion by binding to catenin, regulation of beta-catenin as a differentiative signaling molecule, and promotion of microtubule assembly. In this respect, the rat incisor enamel organ provides a unique tissue for studying the regulation and functions of APC.
authors.affiliation: Harvard School of Dental Medicine, Boston, Massachusetts, USA.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: J M
authors.initials: JM
authors.lastname: Dobeck
authors.forename: B C
authors.initials: BC
authors.lastname: Sorkin
authors.forename: Z
authors.initials: Z
authors.lastname: Skobe
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000378:metabolism*; D025601:Adenomatous Polyposis Coli Protein; D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D004658:Enamel Organ / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D007180:Incisor; D008334:Mandible; D009363:Neoplasm Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014078:Tooth Eruption; D015854:Up-Regulation / Q000502:physiology
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D003598:Cytoskeletal Proteins; D009363:Neoplasm Proteins