Bacterial iron transport: 1H NMR determination of the three-dimensional structure of the gallium complex of pyoverdin G4R, the peptidic siderophore of Pseudomonas putida G4R.
Among the fluorescent Pseudomonas species, Pseudomonas putida is a rare case of a nitrogen-fixing bacterium that transforms nitrogen into ammonia. When grown under iron-deficient conditions, it produces two major pyoverdins: pyoverdin G4R and pyoverdin G4RA. Their primary structures have been established using FAB-MS and one- and two-dimensional 15N, 13C, and 1H NMR on both the unlabeled and 15N-labeled compounds [Salah El Din, A. L. M., et al. (1997) Tetrahedron 53, 12539-12552]. The two pyoverdins have a common chromophore derived from 2,3-diamino-6,7-dihydroxyquinoline. The chromophore is bound to the linear heptapeptide L-Asp-L-Orn-D-beta-threo-OHAsp-L-Dab-Gly-L-Ser-L-cyclo-OHOrn . Circular dichroism spectra suggest that the absolute configuration of the metal complex is Delta. The three-dimensional structure in solution of pyoverdin G4R-Ga(III) was determined after interpretation of two-dimensional 1H NMR spectra recorded at 283 and 303 K. The complex is tightly defined with a compact structure with a Delta absolute configuration. The site of complexation of the metal ion is found to be located on the surface of the molecule, showing that the ion can be released without large conformational changes, while the polar groups of the peptide chain, which may be responsible for the recognition of the receptor, are placed on the opposite side of the overall shape. The three-dimensional structure of pyoverdin G4R-Ga(III) is compared with those of other pyoverdins, and the role of the structure in iron uptake is discussed.
Atkinson
R A
RA
Laboratoire de Chimie Microbienne, Associé au CNRS, UPR 9003 du CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Illkirch, France.
Salah El Din
A L
AL
Kieffer
B
B
Lefèvre
J F
JF
Abdallah
M A
MA
D001426:Bacterial Proteins / Q000737:chemistry*; D001692:Biological Transport; D005708:Gallium / Q000737:chemistry*; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000737:chemistry*; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry*; D010860:Pigments, Biological / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D011522:Protons; D017262:Siderophores / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001426:Bacterial Proteins; D007502:Iron Chelating Agents; D009842:Oligopeptides; D010860:Pigments, Biological; D011522:Protons; D017262:Siderophores; C042453:pyoverdin; D005708:Gallium; D007501:Iron