title: Detection of specific antibodies in saliva during dengue infection.
abstract: Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin M (IgM) and IgG capture enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Duo ELISA). Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition (HAI) assay and an in-house IgM and IgG capture ELISA. With a positive result defined as either salivary IgM or IgG levels above the cutoff value, an overall sensitivity of 92% was obtained for both primary- and secondary-dengue patients (22 of 24), while no patients with non-flavivirus infections (n = 11) and no healthy laboratory donors (n = 17) showed elevation of salivary antidengue antibody (100% specificity). Salivary IgG levels correlated well with serum HAI titer (r = 0.78), and salivary IgG levels could be used to distinguish between primary- and secondary-dengue virus infections.
authors.affiliation: PanBio Pty Ltd., Windsor, Queensland, Australia.
authors.forename: A J
authors.initials: AJ
authors.lastname: Cuzzubbo
authors.forename: D W
authors.initials: DW
authors.lastname: Vaughn
authors.forename: A
authors.initials: A
authors.lastname: Nisalak
authors.forename: S
authors.initials: S
authors.lastname: Suntayakorn
authors.forename: J
authors.initials: J
authors.lastname: Aaskov
authors.forename: P L
authors.initials: PL
authors.lastname: Devine
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011446:Prospective Studies; D012463:Saliva / Q000821:virology*; D012680:Sensitivity and Specificity
chemical_list: D000914:Antibodies, Viral; D007071:Immunoglobulin A, Secretory; D007074:Immunoglobulin G; D007075:Immunoglobulin M