Aqueous two-phase partitioning sample preparation prior to liquid chromatography of hydrophilic drugs in blood.
The technique of aqueous two-phase partitioning is investigated as a sample preparation procedure prior to LC determination of drugs in blood. In the extraction 500 microl of whole blood is added to 2.00 g of PEG 300 and 18.0 g of phosphate buffer. The phases are mixed by stirring and allowed to separate before the clear lower salt phase is drained out using a specially designed glass adapter. After filtration 20 microl is injected into the LC system. When validated for the X-ray contrast medium iohexol R.S.D. was 2.0% and 1.2% at 10 microg/ml and 100 microg/ml of iohexol in blood, respectively.
Agasøster
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Department of Pharmacy, University of Tromsø, Norway.
D000082:Acetaminophen / Q000097:blood*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002021:Buffers; D002417:Cattle; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003287:Contrast Media; D003956:Dialysis; D006801:Humans; D007472:Iohexol / Q000032:analysis*; D010710:Phosphates; D011092:Polyethylene Glycols; D011786:Quality Control; D012680:Sensitivity and Specificity; D013806:Theophylline / Q000097:blood*
D001798:Blood Proteins; D002021:Buffers; D003287:Contrast Media; D010710:Phosphates; D000082:Acetaminophen; D011092:Polyethylene Glycols; D007472:Iohexol; D013806:Theophylline