A new low density lipoprotein receptor related protein, LRP5, is expressed in hepatocytes and adrenal cortex, and recognizes apolipoprotein E.
The isolation and characterization of rabbit and human cDNAs revealed a new low density lipoprotein receptor (LDLR)-related protein (LRP) designated as LRP5. Human LRP5 cDNA encodes a 1, 616-amino acid type I membrane-like protein with three ligand binding repeats in its extracellular region. LDLR-deficient cells transduced by recombinant adenovirus containing human LRP5 exhibited increased binding of apolipoprotein E (apoE)-enriched beta-migrating very low density lipoprotein. Northern blotting and in situ hybridization revealed a high level of LRP5 expression in hepatocytes and the adrenal gland cortex. In LDLR-deficient Watanabe heritable hyperlipidemic rabbits, LRP5 mRNA was increased in the liver and accumulated in cholesterol-laden foam cells of atherosclerotic lesions.
Kim
D H
DH
Tohoku University Gene Research Center, Sendai, 981-8555, Japan.
Inagaki
Y
Y
Suzuki
T
T
Ioka
R X
RX
Yoshioka
S Z
SZ
Magoori
K
K
Kang
M J
MJ
Cho
Y
Y
Nakano
A Z
AZ
Liu
Q
Q
Fujino
T
T
Suzuki
H
H
Sasano
H
H
Yamamoto
T T
TT
D000302:Adrenal Cortex / Q000737:chemistry / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D001057:Apolipoproteins E / Q000737:chemistry / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism; D001665:Binding Sites; D015152:Blotting, Northern; D002784:Cholesterol / Q000378:metabolism; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism; D017403:In Situ Hybridization / Q000379:methods; D026502:LDL-Receptor Related Proteins; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D001057:Apolipoproteins E; D026502:LDL-Receptor Related Proteins; C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D011973:Receptors, LDL; D002784:Cholesterol