[Outbreak of viral meningoencephalitis. Comparison of 2 biological systems used for isolation of the virus].
An increase of the cases of viral meningoencephalitis was reported in our country from October to December, 1995. 23 faeces specimens were received from 2 provinces aimed at obtaining the viral isolation by their inoculation in cell culture and in newborn mice at the same time in order to compare the sensitivity of both systems in isolation. A higher number of isolations was obtained in cell culture. The cell culture from kidney of a normal adult green African monkey (Vero) proved to be more sensitive. 18 isolations were obtained and identified as Coxsackievirus B5. An anatomical and pathological study was conducted in inoculated mice and injuries compatible with the enteroviral infection were observed.
Abreu Nicot
I M
IM
Instituto de Medicina Tropical Pedro Kourí.
Bello Corredor
M
M
Más Lago
P
P
Arteaga Hernández
E
E
Avalos Redón
I
I
Sarmiento Pérez
L R
LR
Palomera Puente
R
R
Pérez Santos
L
L
D000328:Adult; D000818:Animals; D002522:Chlorocebus aethiops; D003384:Coxsackievirus Infections / Q000453:epidemiology* / Q000821:virology; D003462:Cuba / Q000453:epidemiology; D018792:Encephalitis, Viral / Q000453:epidemiology* / Q000821:virology; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D006801:Humans; D008587:Meningitis, Viral / Q000453:epidemiology* / Q000821:virology; D008590:Meningoencephalitis / Q000453:epidemiology* / Q000821:virology; D051379:Mice; D012680:Sensitivity and Specificity; D014709:Vero Cells
Estudio de un brote de meningoencefalitis viral. Comparación de 2 sistemas biológicos empleados para el aislamiento.