On-line coupling of microdialysis sampling with liquid chromatography for the determination of peptide and non-peptide leukotrienes.
An automated on-line sampling method was developed using microdialysis as the simultaneous sampling and sample pre-treatment technique. The extraction fraction values of microdialysis probes sampling different eicosanoids were investigated. The impact of cyclodextrins in the perfusion liquid used for sampling hydrophobic eicosanoids in biological systems was also studied. The total time for one analysis was 7.6 min allowing seven measurements per hour for monitoring kinetic changes in biological systems.
Kjellström
S
S
Department of Analytical Chemistry, University of Lund, Sweden.
Emnéus
J
J
Laurell
T
T
Heintz
L
L
Marko-Varga
G
G
D001322:Autoanalysis; D002460:Cell Line; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D017077:Culture Media, Conditioned; D003505:Cyclodextrins; D015777:Eicosanoids / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D007975:Leukotriene B4 / Q000032:analysis; D017997:Leukotriene C4 / Q000032:analysis; D017998:Leukotriene D4 / Q000032:analysis; D017999:Leukotriene E4 / Q000032:analysis; D015289:Leukotrienes / Q000032:analysis*; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods; D009000:Monocytes / Q000378:metabolism; D011456:Prostaglandins B / Q000032:analysis
D017077:Culture Media, Conditioned; D003505:Cyclodextrins; D015777:Eicosanoids; D007202:Indicators and Reagents; D015289:Leukotrienes; D011456:Prostaglandins B; D007975:Leukotriene B4; D017997:Leukotriene C4; D017998:Leukotriene D4; D017999:Leukotriene E4; C042026:prostaglandin B2