title: Glomerular cell proliferation and apoptosis in uninephrectomized spontaneously hypertensive rats.
abstract: We studied renal function, glomerular cell proliferation and apoptosis for three months after uninephrectomy (UNX) in young, male, spontaneously hypertensive rats (SHR). Apoptosis was assessed by in situ dUTP biotin nick-end labeling method (TUNEL) and by propidium iodide staining. Proliferation rate was determined by immunohistochemistry to proliferating cell nuclear antigen (PCNA). Glomerular bcl-2 expression was assessed by Northern blot analysis. Our results indicate a parallel increase in proliferation and in apoptotic rates in glomerular cells from the first to the second month after UNX. In the third month after UNX, PCNA-labeled cell number continues increasing, whereas TUNEL-labeled cells did not increase. Bcl-2 expression was negative in the first and second months and increased in the third month. Glomerular size and proteinuria increased progressively along the three months of follow-up. Our observations demonstrate a different profile of cell proliferation and apoptosis during the genesis of early glomerular damage in UNX-SHR.
authors.affiliation: Instituto Reina Sofía de Investigación Nefrológica, Departamento de Fisiología y Farmacología, Salamanca, Spain.
authors.forename: A M
authors.initials: AM
authors.lastname: Rodríguez-López
authors.forename: O
authors.initials: O
authors.lastname: Flores
authors.forename: M A
authors.initials: MA
authors.lastname: Arévalo
authors.forename: J M
authors.initials: JM
authors.lastname: López-Novoa
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D001794:Blood Pressure; D015152:Blotting, Northern; D002455:Cell Division / Q000502:physiology; D018076:DNA, Complementary; D015870:Gene Expression / Q000502:physiology; D006977:Hypertension, Renal / Q000503:physiopathology / Q000601:surgery; D020287:In Situ Nick-End Labeling; D007678:Kidney Glomerulus / Q000166:cytology* / Q000601:surgery; D008297:Male; D009392:Nephrectomy; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011918:Rats, Inbred SHR / Q000502:physiology*
chemical_list: D000199:Actins; D018076:DNA, Complementary; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger