Microtiter plate-format optode.
Microtiter plate-format optodes could be assembled by casting bulk-response membranes into the standard 96-well polypropylene-based plate or by screen printing them on an optically transparent substrate with 96-well pattern. The compositions of thick optode membranes, especially the ratios of poly(vinyl chloride) (PVC) to plasticizer [bis-(2-ethylhexyl) sebacate (DOS)], were carefully optimized to provide reproducible and rapid response. Adjusting the ratio of PVC to DOS by 1:6, bulk-response membranes containing neutral carrier (4-tert-butyl calix[4]arene tetraacetic acid tetraethyl ester for sodium-selective membrane or valinomycin for potassium-selective membrane) and lipophilic pH indicator (ETH 5294) could exhibit equilibrium response in 5 min. The practical utility of microtiter plate-format optodes has been examined by determining clinically relevant electrolytes in serum samples. It was demonstrated that microtiter plate-format optodes can provide high sample throughput (approximately 100 samples in less than 5 min), analytical performance comparable to that of a potentiometric clinical analyzer, and additional information on electrolytes using the same samples prepared for other colorimetric measurements.
Kim
S B
SB
Department of Chemistry, Kwangwoon University, Seoul, Korea.
Cho
H C
HC
Cha
G S
GS
Nam
H
H
D000818:Animals; D002138:Calibration; D004566:Electrodes; D004573:Electrolytes / Q000097:blood; D006493:Heparin / Q000032:analysis; D006736:Horses; D006801:Humans; D007202:Indicators and Reagents; D008567:Membranes, Artificial
D004573:Electrolytes; D007202:Indicators and Reagents; D008567:Membranes, Artificial; D006493:Heparin