Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies.
Protein spots, such as immunoglobulin G (IgG), A (IgA) or alpha 2-macroglobulin, on the patterns of non-denaturing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) were selectively removed by the application of protein A or antibody-conjugated protein A agarose to the first-dimension gel top at the cathode side. A protein residing at pI 5.8 and with a molecular mass of about 200 kDa was clearly detected when the IgG and IgA spots were removed from the 2-D PAGE pattern.
Shimazaki
Y
Y
Department of Material Science, Faculty of Science, Ehime University, Mastuyama-city, Japan. yoji@dpc.ehime-u.ac.jp
Ohara
S
S
Manabe
T
T
D000906:Antibodies; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007526:Isoelectric Point; D008970:Molecular Weight; D011489:Protein Denaturation; D011506:Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D012685:Sepharose; D013205:Staphylococcal Protein A; D000511:alpha-Macroglobulins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification
D000906:Antibodies; D001798:Blood Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D011506:Proteins; D013205:Staphylococcal Protein A; D000511:alpha-Macroglobulins; D012685:Sepharose