title: Effects of hydroxyethyl-starch-bound deferoxamine on ischemia/reperfusion injury in chronic nerve compression.
abstract: The authors have demonstrated previously that pretreatment with deferoxamine, an iron chelator and antioxidant, at the time of release in acute nerve compression, provided protection against ischemia/reperfusion (I/R) injury. In the present study, they evaluated whether therapeutic intervention with hydroxyethyl-starch-bound deferoxamine (HES-DFO) at the time of release of the chronically-compressed peripheral nerve protects the nerve from I/R injury. The sciatic nerves of 43 male Sprague-Dawley rats, weighing 325 to 350 g, were subjected to 8 weeks of compression with Silastic tubing. The treatment group received intravenous HES-DFO (70 mg/kg) at the time of decompression, while the control group received an equal volume of intravenous hetastarch vehicle at the same time schedule and route. Nerve-tissue samples from the compression site, as well as contralateral noncompressed nerves, were assayed for malondialdehyde (MDA), a marker of I/R injury. The control group exhibited MDA levels up to five times normal, and did not return to normal for 21 days. In contrast, the HES-DFO group had MDA levels that were not statistically significantly different from normal levels. The results confirm that pretreatment with HES-DFO prior to the surgical decompression of chronically-compressed nerve provides marked protection against I/R injury.
authors.affiliation: Division of Plastic, Reconstructive and Maxillofacial Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Marin
authors.forename: M J
authors.initials: MJ
authors.lastname: Im
authors.forename: J A
authors.initials: JA
authors.lastname: Girotto
authors.forename: G
authors.initials: G
authors.lastname: Borschel
authors.forename: K D
authors.initials: KD
authors.lastname: Bickel
mesh_terms: D000818:Animals; D003676:Deferoxamine / Q000627:therapeutic use*; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use*; D007502:Iron Chelating Agents / Q000627:therapeutic use*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009408:Nerve Compression Syndromes / Q000601:surgery*; D011292:Premedication; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000517:prevention & control*; D012584:Sciatic Nerve / Q000098:blood supply / Q000378:metabolism
chemical_list: D006895:Hydroxyethyl Starch Derivatives; D007502:Iron Chelating Agents; C068460:hydroxyethyl starch-deferoxamine conjugate; D008315:Malondialdehyde; D003676:Deferoxamine