title: The hemagglutination-positive phenotype of Mycoplasma synoviae induces experimental infectious synovitis in chickens more frequently than does the hemagglutination-negative phenotype.
abstract: Inoculation with hemagglutination-positive (HA+) cultures of Mycoplasma synoviae AAY-4 induced acute synovitis significantly more frequently (P = 0.001) in chicken tibiotarsal-tarsometatarsal joints than did inoculation with HA-negative (HA-) cultures derived from the same clone of AAY-4. Immunoblotting analyses showed that HA+ cultures abundantly expressed two phase-variable hemadherence-associated surface membrane proteins of 53 kDa and 48 to 50 kDa defined by monoclonal antibodies. HA- cultures lacked the 53-kDa proteins and synthesized truncated 27- to 30-kDa forms of the 48- to 50-kDa proteins. Inoculation of cyclosporin A (CsA) into infected joints significantly decreased the frequency of acute synovitis (P = 0.001). Moreover, repeated intra-articular inoculation of CsA (three doses of 1 mg at 2-day intervals) significantly reduced the local antibody response to M. synoviae in the joints treated with CsA.
authors.affiliation: Department of Animal Science, Biotechnical Faculty, University of Ljubljana, 1230 Domzale, Slovenia.
authors.forename: M
authors.initials: M
authors.lastname: Narat
authors.forename: D
authors.initials: D
authors.lastname: Bencina
authors.forename: S H
authors.initials: SH
authors.lastname: Kleven
authors.forename: F
authors.initials: F
authors.lastname: Habe
mesh_terms: D000818:Animals; D001168:Arthritis / Q000276:immunology / Q000382:microbiology*; D002645:Chickens; D016572:Cyclosporine / Q000494:pharmacology; D006388:Hemagglutinins; D006614:Hindlimb; D007166:Immunosuppressive Agents / Q000494:pharmacology; D009174:Mycoplasma / Q000145:classification / Q000472:pathogenicity*; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology; D013582:Synovial Fluid / Q000166:cytology; D013583:Synovial Membrane / Q000473:pathology; D013585:Synovitis / Q000276:immunology / Q000382:microbiology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D006388:Hemagglutinins; D007166:Immunosuppressive Agents; D016572:Cyclosporine