The carboxy-terminal end of the candidate tumor suppressor gene HIC-1 is phylogenetically conserved.
HIC-1 (hypermethylated in cancer), a new candidate tumor suppressor gene located in 17p13.3, encodes a protein with five Krüppel-like C2H2 zinc finger motifs and a N-terminal protein-protein interaction domain called BTB/POZ. These two domains appeared as the only conserved regions found between human HIC-1 and its avian homologue, gammaFBP-B, isolated as a transcriptional repressor of the gammaF-Crystallin gene. We have recloned the HIC-1 gene and found four nucleotide differences within the 3' part of its published coding sequence. The corrected HIC-1 C-terminal end exhibits now significant homology (70%) to the chicken gammaFBP-B C-terminal end and appears thus as a third phylogenetically conserved domain that may serve an important, yet unknown function in HIC-1.
Deltour
S
S
CNRS URA 1160, Institut de Biologie de Lille, Institut Pasteur de Lille, 1 Rue Calmette, 59021 Lille Cedex, France.
Guerardel
C
C
Stehelin
D
D
Leprince
D
D
D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D016147:Genes, Tumor Suppressor; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*
C096870:Hic1 protein, mouse; D051741:Kruppel-Like Transcription Factors; D011994:Recombinant Proteins; D014157:Transcription Factors