title: Neuropeptide Y release in the paraventricular nucleus is decreased during transient hyperphagia induced by microinjection of colchicine into the ventromedial nucleus of rats.
abstract: Disruption of neural signaling in the ventromedial nucleus (VMN) of rats by microinjection of the neurotoxin colchicine (COL) results in transient hyperphagia accompanied by enhanced weight gain. We tested the hypothesis that release of neuropeptide Y (NPY), a potent orexigenic signal is augmented within the paraventricular nucleus (PVN) of COL-treated hyperphagic rats. Adult male rats were microinjected bilaterally with either COL (4 microg/0.5 microl in saline) or saline in the VMN and a push-pull guide cannula aimed at the PVN was implanted for analysis of extra-cellular NPY. COL-injected rats gained 37.8+/-6.1 g while the saline-injected rats lost 9.3+/-3.4 g during the 4 days following surgery. On day 4, post-injection, the PVN of these rats was perfused with artificial cerebrospinal fluid via the push-pull cannula. NPY levels in perfusates collected at 10 min intervals from hyperphagic, COL-injected rats were markedly diminished. Cumulative NPY efflux over the 180 min sampling period was significantly less in COL-treated (27.7+/-6.0 pg) versus saline-injected control rats (110.6+/-32.2 pg; P < 0.05). These results show that impairment of neural signaling in the VMN by COL suppressed NPY release in the PVN. These observations taken together with previous studies showing diminution in preproNPY mRNA in the arcuate nucleus (ARC) and NPY levels in the PVN are in accordance with the thesis that the VMN normally exerts a facilitatory influence on NPYergic signaling in the ARC-PVN axis.
authors.affiliation: Department of Physiology, University of Florida, College of Medicine, Gainesville 32610-0274, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Jain
authors.forename: M G
authors.initials: MG
authors.lastname: Dube
authors.forename: S P
authors.initials: SP
authors.lastname: Kalra
authors.forename: P S
authors.initials: PS
authors.lastname: Kalra
mesh_terms: D000818:Animals; D003078:Colchicine / Q000008:administration & dosage / Q000494:pharmacology; D006963:Hyperphagia / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D008845:Microinjections; D009478:Neuropeptide Y / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009498:Neurotoxins / Q000008:administration & dosage / Q000494:pharmacology; D010286:Paraventricular Hypothalamic Nucleus / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015430:Weight Gain / Q000502:physiology
chemical_list: D009478:Neuropeptide Y; D009498:Neurotoxins; D003078:Colchicine