Protein and cell engineering of components of the human immunoglobulin E receptor/effector system: applications for therapy and diagnosis.
Adaptive immune responses characterised by the synthesis of antibodies of the immunoglobulin E (IgE) isotype play an important role in type I hypersensitivity disorders and parasitic infestations, diseases which have an significant socioeconomic impact world-wide. This paper considers potential applications of recent advances in our understanding of the origin of isotype specific immune responses which emerged as a result of cell and protein engineering studies on components of the human IgE/receptor/effector system. Furthermore, the identification of the receptor binding regions in IgE as a result of the development of a stable assay system has important applications for the design of rational therapeutic interventions in allergy and asthma, the treatment of mast cell tumours, and the establishment of procedures for the selective isolation of cells expressing the high-affinity receptor for IgE for functional studies.
Helm
B A
BA
Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, The University of Sheffield, UK. B.Helm@Sheffield.AC.UK
Sayers
I
I
Swan
J
J
Smyth
L J
LJ
Cain
S A
SA
Suter
M
M
Machado
D C
DC
Spivey
A C
AC
Padlan
E A
EA
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D002460:Cell Line; D015195:Drug Design; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D007073:Immunoglobulin E / Q000737:chemistry* / Q000276:immunology*; D008407:Mast Cells / Q000276:immunology; D010272:Parasitic Diseases / Q000175:diagnosis / Q000276:immunology* / Q000628:therapy; D015202:Protein Engineering / Q000379:methods*; D051381:Rats; D017455:Receptors, IgE / Q000737:chemistry*
D000906:Antibodies; D017455:Receptors, IgE; D007073:Immunoglobulin E