[High homology of plasmids carrying class II restriction modification systems, EcoRV isoschizomers, and their prevalence in natural Escherichia coli strains].
Screening of 660 clinical Enterbacteriaceae strains from the collection of L. V. Gromashevsky Kiev Institute of Epidemiology and Infectious Diseases for specific endonuclear activity revealed site-specific endonucleases, EcoRV isoschizomers in 6 E. coli strains. Genes coding for endonucleases and methyltransferases were localized on small (6.2 kb) multicopy Hsd+ plasmids. All plasmids were successfully transferred in laboratory strain E. coli K802. Restriction analysis and subcloning showed no differences in the structural and functional organization of studied plasmids and a previously revealed pLB1 plasmid [3,10], this reflecting their high homology, if not identity. These data allow us to propose effective horizontal transfer of EcoRV plasmids among natural E. coli isolates in the region studied.
Kravets
A N
AN
Pertsev
A V
AV
Tarutina
Z E
ZE
Solonin
A S
AS
D002874:Chromosome Mapping; D015252:Deoxyribonucleases, Type II Site-Specific / Q000737:chemistry / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D016680:Genome, Bacterial; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D014455:Ukraine
D007527:Isoenzymes; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases
Vysokaia gomologiia plasmid, nesushchikh geny sistemy restriktsii-modifikatsii II klassa--izoshizomerov EcoRV, i ikh rasprostranenie v prirodnykh shtammakh Echerichia coli.