title: Alterations in ultraspiracle (USP) content and phosphorylation state accompany feedback regulation of ecdysone synthesis in the insect prothoracic gland.
abstract: Insect molting and metamorphosis are elicited by a class of ecdysteroids, mainly 20-hydroxyecdysone (20E), the precursor of which is synthesized in the prothoracic gland. 20E acts via the ecdysone receptor (EcR) and its heterodimer partner ultraspiracle (USP). Analysis of the prothoracic gland of Manduca sexta revealed that the developmental expression and phosphorylation of a specific USP form, p47, is positively correlated with ecdysteroidogenesis and that 20E, but not ecdysone, is responsible for initiating the translational expression and phosphorylation of p47. The latter forms a functional complex with EcR and the ligand-complex interaction results in the down regulation of ecdysteroidogenesis and the inhibition of prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis. The composite data suggest that USP plays a key role in modulating PTTH-stimulated ecdysteroid biosynthesis through the selective expression and phosphorylation of the p47 USP isoform.
authors.affiliation: Department of Biology, University of North Carolina, Chapel Hill 27599-3280, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Song
authors.forename: L I
authors.initials: LI
authors.lastname: Gilbert
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015536:Down-Regulation; D004441:Ecdysterone / Q000096:biosynthesis*; D007301:Insect Hormones / Q000037:antagonists & inhibitors*; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics; D018402:Manduca / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D011233:Precipitin Tests
chemical_list: D007301:Insect Hormones; D019476:Insect Proteins; D004441:Ecdysterone; C009089:prothoracicotropic hormone