Covalent photolinker-mediated immobilization of an intermediate dextran layer to polymer-coated surfaces for biosensing applications.
A new method is presented for the covalent binding of dextran as an intermediate layer on surface acoustic wave (SAW) devices. For biosensing applications in aqueous media commercially available SAW devices require surface passivation to prevent corrosion of the aluminum device structures in electrolytes. Thin films of polyimide and parylene revealed exceptional passivation properties. They were used as a base for dextran immobilization. Covalent binding of dextran to polymer-coated surfaces was achieved by photoimmobilization. Aryldiazirine-functionalized bovine serum albumin served as the multifunctional light-activable linking agent (photolinker polymer). Dextran and photolinker polymer were mixed and photobonded to sensor surfaces. Essential photoimmobilization parameters were optimized. The binding of proteins to dextran applying carbodiimide chemistries was exemplified with antiurease antibodies and the feasibility of specific immunosensing was investigated on SAW sensors connected to a fluid handling system.
Barié
N
N
Forschungszentrum Karlsruhe GmbH, Institut für Instrumentelle Analytik, Germany. nicole.barie@ifia.fzk.de
Rapp
M
M
Sigrist
H
H
Ache
H J
HJ
D000162:Acoustics; D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003911:Dextrans; D008027:Light; D011108:Polymers
D003911:Dextrans; D011108:Polymers