Discrimination of Helicobacter pullorum and Campylobacter lari by analysis of whole cell fatty acid extracts.
Helicobacter pullorum and Campylobacter lari are rarely isolated from humans with acute enteritis. Hitherto the two species could only be identified by genotypic techniques. Gas liquid chromatography of whole cell fatty acid extracts is described as the first phenotypic method for discrimination of the two species. Cholesteryl glucoside, a characteristic feature of the genus Helicobacter, but seldom found in other bacteria, could not be detected in Helicobacter pullorum. Therefore, rapid determination of this glycolipid may serve as a discrimination marker for Helicobacter pullorum from most other Helicobacter species.
Steinbrueckner
B
B
Institut fuer Medizinische Mikrobiologie und Hygiene, Universitaet Freiburg, Germany.
Haerter
G
G
Pelz
K
K
Burnens
A
A
Kist
M
M
D000818:Animals; D002167:Campylobacter / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D002645:Chickens / Q000382:microbiology; D002784:Cholesterol / Q000031:analogs & derivatives / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D005227:Fatty Acids / Q000032:analysis*; D016998:Helicobacter / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D006801:Humans; D012623:Seawater / Q000382:microbiology
D005227:Fatty Acids; C027578:cholesteryl glucoside; D002784:Cholesterol