The role of the nirQOP genes in energy conservation during anaerobic growth of Pseudomonas aeruginosa.
The nirQOP operon, which is located between the genes for nitrite reductase and nitric oxide reductase in Pseudomonas aeruginosa, encodes a putative ATP-binding protein and two putative transmembrane proteins. Phylogenetic analysis showed that NirO belongs to the family of subunit III of cytochrome oxidases but is distantly related to the other bacterial or mitochondrial proteins. P. aeruginosa strains that lacked the nirOP genes had all enzyme activities for denitrification and could grow under anaerobic conditions with nitrate or nitrite as an electron acceptor. However, the energy conservation efficiency of anaerobic respiration was lower in these strains than in strains harboring the nirOP gene.
Arai
H
H
Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Kodama
T
T
Igarashi
Y
Y
D000595:Amino Acid Sequence; D000693:Anaerobiosis; D003576:Electron Transport Complex IV / Q000235:genetics; D004734:Energy Metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D020161:Physical Chromosome Mapping; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000254:growth & development*
D009566:Nitrates; D009573:Nitrites; D003576:Electron Transport Complex IV