title: On the absence of the glyceraldehyde 3-phosphate/pyruvate pathway for isoprenoid biosynthesis in fungi and yeasts.
abstract: The biosynthesis of isopentenyl diphosphate, the central intermediate of isoprenoid formation, was investigated in the fungus Aschersonia aleyrodis and the yeast Rhodotorula glutinis. The incorporation of 13C-labeled glucose or acetate into their isoprenoids showed that ergosterol in both micro-organisms, ubiquinone in R. glutinis and dihydro-ubiquinone, beta-carotene and triterpenes of the hopane series in A. aleyrodis were synthesized via the mevalonate pathway. No evidence for the presence of the alternative mevalonate-independent glyceraldehyde 3-phosphate/pyruvate pathway was found.
authors.affiliation: Université Louis Pasteur/CNRS, Institut Le Bel, Strasbourg, France.
authors.forename: A
authors.initials: A
authors.lastname: Disch
authors.forename: M
authors.initials: M
authors.lastname: Rohmer
mesh_terms: D000085:Acetates / Q000378:metabolism; D002247:Carbon Isotopes; D004875:Ergosterol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005986:Glyceraldehyde 3-Phosphate / Q000378:metabolism*; D045782:Hemiterpenes; D006999:Hypocreales / Q000378:metabolism*; D008798:Mevalonic Acid / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*; D012248:Rhodotorula / Q000378:metabolism*; D014315:Triterpenes / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; D011773:Pyruvates; D014315:Triterpenes; D019207:beta Carotene; D014451:Ubiquinone; D005986:Glyceraldehyde 3-Phosphate; C004809:isopentenyl pyrophosphate; D005947:Glucose; D008798:Mevalonic Acid; D004875:Ergosterol