title: Gentamicin resistance in clinical strains of Enterobacteriaceae associated with reduced gentamicin uptake.
abstract: Seven strains of Enterobacteriaceae resistant to gentamicin obtained as representatives of the predominant resistance profiles in the clinical laboratories of Rafeidia and Al-Watani Hospitals in Nablus (Palestine) were included. Five strains showed a broad aminoglycoside resistance profile but contained no evidence of gentamicin acetylation, adenylation, or phosphorylation. Gentamicin uptake in two tested strains was significantly reduced, compared to that of gentamicin-sensitive E. coli (MIC, 0.5 microgram/mL). These strains are likely resistant due to a relative reduction of the amount of gentamicin and other aminoglycosides entering the bacterial cell. Two strains showed evidence of adenyltransferase ANT(2")-I activity.
authors.affiliation: Department of Biological Sciences, An-Najah N. University, Nablus, Palestine. adwan@najah.edu
authors.forename: K
authors.initials: K
authors.lastname: Adwan
authors.forename: N
authors.initials: N
authors.lastname: Abu-Hasan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D004755:Enterobacteriaceae / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005839:Gentamicins / Q000378:metabolism* / Q000494:pharmacology*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects / Q000235:genetics; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D011511:Proteus / Q000187:drug effects / Q000235:genetics; D011532:Providencia / Q000187:drug effects / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins