title: [Establishing an umbilical cord blood bank for unrelated allogenic stem cell transplantation].
abstract: We report the establishment of a cord-blood bank in a routine hematological laboratory. Cord-blood collection was performed with placenta in utero by a trained team and immediately sent to the cord-blood bank. There, 6.8 ml cord-blood was used for analysis of nucleated cell counts, counts of CD34-positive cells, CFU's, complete HLA-typing, ABO and Rhesus blood groups, bacteriologic cultures and serology for HIV 1 and 2, HbsAg, HVC, CMV, syphilis and toxoplasmosis. The cord-blood collection was frozen and conserved at -192 degrees C. From each cord-blood vials of DNA, viable cells and plasma were cryopreserved. Between June 1997 and April 1998, 54 cord-bloods were collected. 40 of them were cryo-preserved, and 14 discarded because of low cell counts. The median volume was 109 ml with 1.4 x 10(9) nucleated cells. The in vitro capacity of proliferation of the cord-blood correlated well with the absolute counts of CD34-positive cells (r = 0.93), moderately with the relative counts of CD34 (r = 0.68) as well as the nucleated cells (r = 0.70), poorly with the volume (r = 0.44). Three of the 40 (7.5%) cord-blood products contained a bacterial contamination. This study shows that a cord-blood bank can be organised in a routine hematological laboratory, which is familiar with transplantation products. However, the procedure is time consuming, expensive and requires a highly qualified team and specialised technical equipment.
authors.affiliation: Departement Zentrallaboratorium, Kantonsspital Basel.
authors.forename: A
authors.initials: A
authors.lastname: Tichelli
authors.forename: D
authors.initials: D
authors.lastname: Surbek
authors.forename: H
authors.initials: H
authors.lastname: Huxol
authors.forename: C
authors.initials: C
authors.lastname: Schmolck
authors.forename: L
authors.initials: L
authors.lastname: John
authors.forename: R
authors.initials: R
authors.lastname: Wicki
authors.forename: T
authors.initials: T
authors.lastname: Hoffmann
authors.forename: A
authors.initials: A
authors.lastname: Wodnar-Filipowicz
authors.forename: J
authors.initials: J
authors.lastname: Passweg
authors.forename: T
authors.initials: T
authors.lastname: Kühne
authors.forename: P
authors.initials: P
authors.lastname: Imbach
authors.forename: W
authors.initials: W
authors.lastname: Holzgreve
authors.forename: A
authors.initials: A
authors.lastname: Gratwohl
mesh_terms: D001771:Blood Banks / Q000458:organization & administration*; D005312:Fetal Blood / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007231:Infant, Newborn; D010348:Patient Care Team / Q000458:organization & administration; D013557:Switzerland
vernacular_title: Aufbau einer Nabelschnurblutbank für unverwandte allogene Stammzelltransplantationen.