Performance of the Dynabeads anti-Salmonella system in the detection of Salmonella species in foods, animal feeds, and environmental samples.
The immunomagnetic separation Dynabeads anti-Salmonella technique was evaluated for the detection of Salmonella species in a variety of foods, feeds, and environmental samples. Salmonella cells in preenrichment broths were captured using the Dynabeads anti-Salmonella system and were then washed and plated on indicator media. A total of 308 naturally contaminated samples were analyzed, including 46 cheese and egg products, 183 animal feeds, and 79 environmental swabs. The results of the Dynabeads method gave 100% correlation with the results of the standard culture technique used for foods and the modified semisolid Rappaport-Vassiliadis method used for feeds and environmental samples.
Shaw
S J
SJ
Canadian Food Inspection Agency, Ottawa, Ontario, Canada. sshaw@em.agr.ca
Blais
B W
BW
Nundy
D C
DC
D000821:Animal Feed / Q000382:microbiology*; D000907:Antibodies, Bacterial; D001431:Bacteriological Techniques; D004783:Environmental Microbiology; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000302:isolation & purification; D006801:Humans; D018189:Immunomagnetic Separation; D012475:Salmonella / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012480:Salmonella Infections / Q000382:microbiology
D000907:Antibodies, Bacterial