title: [Isolation of anti-Cryptococcus neoformans immunoglobulin G].
abstract: Two immunization schemes were applied to rabbits with an autoctonous strain aimed at obtaining an anti-Crytococcus neoformans IgG. Whole cells treated with formalin were used. Dosages and immunization routes were different. The sera obtained were titered and that with the highest titer was selected (1:1 024 by the laminar agglutination method) and purified by saline precipitation and ion exchange chromatography. An IgG of high purity and biological activity was obtained.
authors.affiliation: Instituto de Medicina Tropical Pedro Kourí.
authors.forename: M T
authors.initials: MT
authors.lastname: Illnait Zaragozí
authors.forename: G
authors.initials: G
authors.lastname: Martínez Machín
authors.forename: C
authors.initials: C
authors.lastname: Fernández Andreu
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000276:immunology*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D011817:Rabbits
chemical_list: D000908:Antibodies, Fungal; D007074:Immunoglobulin G
vernacular_title: Obtención de inmunoglobulina G anti-Cryptococcus neoformans.