Comparative studies on peptides representing the so-called tachykinin-like region of the Alzheimer Abeta peptide [Abeta(25-35)].
In an attempt to answer the question of whether or not the so-called tachykinin-like region of the Alzheimer beta-amyloid protein [Abeta(25-35)] can act as a tachykinin, the sequences Abeta(25-35), Abeta(25-35)amide and their norleucine-35 and phenylalanine-31 analogues were synthesized. These peptides were examined with ligand binding studies, electron microscopy, CD and NMR. In all cases some differences were found between the Abeta(25-35) analogue and the corresponding Phe31 peptide. In addition, in ligand displacement studies on tachykinin NK1 receptors, only the Phe31 analogue showed activity comparable to that of genuine tachykinins. We conclude that peptides based on Abeta(25-35) but with a Phe residue at position 31 do display properties typical of a tachykinin, but that peptides with Ile at this position do not.
El-Agnaf
O M
OM
The Centre for Peptide and Protein Engineering, School of Biology and Biochemistry, The Queen's University of Belfast, 97 Lisburn Road, Belfast BT9 7BL, N. Ireland, U.K.
Irvine
G B
GB
Fitzpatrick
G
G
Glass
W K
WK
Guthrie
D J
DJ
D000544:Alzheimer Disease; D000577:Amides; D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism*; D000818:Animals; D002942:Circular Dichroism; D004600:Eledoisin / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015287:Neurokinin B / Q000378:metabolism; D009646:Norleucine; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D010649:Phenylalanine; D051381:Rats; D013373:Substance P / Q000378:metabolism; D015320:Tachykinins / Q000737:chemistry / Q000378:metabolism
D000577:Amides; D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D015320:Tachykinins; C074636:amyloid beta-protein (25-35); D013373:Substance P; D010649:Phenylalanine; D004600:Eledoisin; D009646:Norleucine; D015287:Neurokinin B