title: Common secondary structures for the second internal transcribed spacer pre-rRNA of two subfamilies of trichostrongylid nematodes.
abstract: Sequences of the second internal transcribed spacer ribosomal DNA for the parasitic trichostrongylid nematodes Trichostrongylus probolurus, Trichostrongylus rugatus and Camelostrongylus mentulatus were compared with previously published sequences for five other species within the genus Trichostrongylus. The secondary structures of the second internal transcribed spacer pre-rRNA for these nematodes were predicted using an energy minimisation method. The results indicate that a common secondary structure of the second internal transcribed spacer of these nematodes is maintained despite distinct differences in primary sequence between species. Sequence differences among Trichostrongylus species ranged from 1.3 to 7.6%, but each species differed by 22-26% in sequence when compared with C. mentulatus which belongs to a different subfamily.
authors.affiliation: Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia. nbc@clyde.its.unimelb.edu.au
authors.forename: N B
authors.initials: NB
authors.lastname: Chilton
authors.forename: H
authors.initials: H
authors.lastname: Hoste
authors.forename: L A
authors.initials: LA
authors.lastname: Newton
authors.forename: I
authors.initials: I
authors.lastname: Beveridge
authors.forename: R B
authors.initials: RB
authors.lastname: Gasser
mesh_terms: D000818:Animals; D001483:Base Sequence; D018509:DNA, Helminth / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012322:RNA Precursors / Q000737:chemistry* / Q000235:genetics; D018510:RNA, Helminth / Q000737:chemistry* / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000737:chemistry* / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000737:chemistry* / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014158:Transcription, Genetic; D014251:Trichostrongyloidea / Q000235:genetics*; D014254:Trichostrongylus / Q000235:genetics*
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal; D012322:RNA Precursors; D018510:RNA, Helminth; D012339:RNA, Ribosomal, 28S; D012340:RNA, Ribosomal, 5.8S