A micromethod for protein assay using a light microscope.
A micromethod which utilizes a protein-dye binding reaction on an acetate membrane and a light microscope with an automatic exposure instrument has been developed for measuring small amounts of protein from limited biological materials. After discoidal cellulose acetate membranes (2.0 mm diameter), which absorbed 0.5 microliter of protein solution containing 0.2-2.0 micrograms of protein, were stained with Coomassie Brilliant Blue G-250, they were examined by a light microscope with an automatic exposure instrument. A linear relation was confirmed between the intensity of dye binding to protein and exposure time under specific optical conditions.
Matsuzawa
T
T
Laboratory of Biology, Obihiro University of Agriculture, Hokkaido, Japan.
Takekoshi
M
M
Aikawa
J
J
D000818:Animals; D002417:Cattle; D002482:Cellulose / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D008297:Male; D008567:Membranes, Artificial; D008832:Microchemistry / Q000379:methods*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods / Q000662:veterinary; D011506:Proteins / Q000032:analysis*; D051381:Rats; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine / Q000032:analysis; D013094:Spermatozoa / Q000737:chemistry
D007202:Indicators and Reagents; D008567:Membranes, Artificial; D011506:Proteins; D012394:Rosaniline Dyes; D012710:Serum Albumin, Bovine; C005062:acetylcellulose; D002482:Cellulose; C004692:coomassie Brilliant Blue