title: Bond strength to dentin with artificial carious lesions: influence of caries detecting dye.
abstract: OBJECTIVE
  To evaluate the influence of dyes for caries detection on tensile bond strength of adhesive materials to artificial carious dentin.
  
  
  METHODS
  Buccal and lingual enamel of human molars were removed leaving intact dentin surfaces. The entire surface of each specimen was covered with nail varnish, keeping a window area of 4 x 4 mm. Artificial carious lesions were induced with acidified gel. Three dyes (0.5% basic fuchsin; Caries Finder and Cari-D-Tect) were used according to manufacturers' recommendations. Specimens were etched with 35% phosphoric acid for 20 s, washed and dried, leaving a wet dentin surface. The adhesive system (Prime & Bond 2.0) was applied in two layers and light-cured. Restorative materials (TPH Spectrum, Dyract, Advance) were bonded using a 3-mm diameter inverted-cone mold. Control groups were made without dye. Eight samples were tested for each group. After 24 hrs of storage in distilled water, the samples were debonded using a testing machine at 0.5 mm/min crosshead speed.
  
  
  RESULTS
  ANOVA and Tukey-Kramer test showed that TPH Spectrum (0.73 MPa) and Dyract (0.74 MPa) had similar bond strengths, and both were higher than Advance (0.0 MPa), which was statistically different (P < 0.01). The use of the dyes did not cause any changes in tensile bond strength for any tested materials.
authors.affiliation: Depto. de Dentística, Faculdade de Odontologia, Universidade de São Paulo, Cid. Universitária, Brazil.
authors.forename: R G
authors.initials: RG
authors.lastname: Palma
authors.forename: M L
authors.initials: ML
authors.lastname: Turbino
authors.forename: E
authors.initials: E
authors.lastname: Matson
authors.forename: J M
authors.initials: JM
authors.lastname: Powers
mesh_terms: D000704:Analysis of Variance; D004396:Coloring Agents / Q000737:chemistry*; D020770:Compomers; D003188:Composite Resins; D001840:Dental Bonding; D003731:Dental Caries / Q000175:diagnosis / Q000628:therapy; D003732:Dental Caries Activity Tests; D003804:Dentin / Q000737:chemistry; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005899:Glass Ionomer Cements; D006801:Humans; D008422:Materials Testing; D008689:Methacrylates; D012394:Rosaniline Dyes / Q000737:chemistry; D017640:Silicates; D013718:Tensile Strength; D017001:Tooth Demineralization
chemical_list: C094366:Advance cement; D004396:Coloring Agents; D020770:Compomers; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C088486:Dyract; D005899:Glass Ionomer Cements; D008689:Methacrylates; C088833:Prisma TPH resin composite; D012394:Rosaniline Dyes; D017640:Silicates