Identification of laminin-10/11 as a strong cell adhesive complex for a normal and a malignant human epithelial cell line.
Laminins are heterotrimeric proteins of basement membranes. More than 50 different trimers may exist. Laminin-10 (alpha5beta1gamma1 rather than laminin-1 (alpha1beta1gamma1) could be the most abundant isoform in the adult stage, and laminin-10 is made by several developing epithelial sheets. We show here that a much used commercial human preparation contains laminin-10 (alpha5beta1gamma1), some laminin-11 (alpha5beta2gamma1), but no laminin-1. Moreover, the laminin-10/11 mixture was found to be a strong adhesive for two human cell lines derived from epithelia. Antibodies against integrin beta1, alpha6 or alpha3 (at 50 microgram/ml) or dystroglycan did not inhibit cell attachment to laminin-10/11, although lower concentrations of anti-dystroglycan and integrin alpha6 antibodies inhibited cell binding to laminin-1.
Ferletta
M
M
Department of Animal Physiology, Uppsala University Biomedical Center, BOX 596, SE-75124 Uppsala, Sweden.
Ekblom
P
P
D000906:Antibodies / Q000494:pharmacology; D015153:Blotting, Western; D002448:Cell Adhesion / Q000276:immunology / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000276:immunology / Q000378:metabolism; D002461:Cell Line, Transformed; D048429:Cell Size / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000276:immunology / Q000378:metabolism; D049030:Dystroglycans; D004591:Electrophoresis, Polyacrylamide Gel; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D016023:Integrins / Q000276:immunology / Q000378:metabolism; D007797:Laminin / Q000737:chemistry* / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight; D020033:Protein Isoforms / Q000737:chemistry / Q000378:metabolism; D017474:Receptors, Laminin / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D000906:Antibodies; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D005353:Fibronectins; D016023:Integrins; D007797:Laminin; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; D017474:Receptors, Laminin; D049030:Dystroglycans