title: Method for analysis of exhaled air by microwave energy desorption coupled with gas chromatography-flame ionization detection-mass spectrometry.
abstract: A method for chemical analysis of volatile constituents in exhaled air of mechanically ventilated patients is described. Exhaled substances are adsorbed and concentrated onto activated charcoal, desorbed by microwave energy and transferred into a gas chromatograph for separation without prior cryofocusing. Substances are identified by flame ionisation detection and mass spectrometry. This method yields reproducible results and is well suited for clinical studies.
authors.affiliation: Department of Anaesthesia and Intensive Care Medicine, University Hospital of Freiburg, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Mueller
authors.forename: J
authors.initials: J
authors.lastname: Schubert
authors.forename: A
authors.initials: A
authors.lastname: Benzing
authors.forename: K
authors.initials: K
authors.lastname: Geiger
mesh_terms: D000114:Acetylene / Q000032:analysis; D000208:Acute Disease; D000327:Adsorption; D018685:Anesthetics, Inhalation / Q000032:analysis; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D002248:Carbon Monoxide / Q000032:analysis; D002606:Charcoal; D004980:Ethane / Q000032:analysis; D005030:Ethylenes / Q000032:analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D007530:Isoflurane / Q000032:analysis; D008171:Lung Diseases / Q000378:metabolism; D008872:Microwaves; D015203:Reproducibility of Results; D012121:Respiration, Artificial; D014835:Volatilization
chemical_list: D018685:Anesthetics, Inhalation; D005030:Ethylenes; D002245:Carbon Dioxide; D002606:Charcoal; D002248:Carbon Monoxide; C036216:ethylene; D007530:Isoflurane; D004980:Ethane; D000114:Acetylene