Tobacco BY-2 cells excise both 3-methyladenine and 7-methylguanine from methylated DNA.
Two of the major products in DNA resulting from exposure to alkylating agents are 7-methylguanine and 3-methyladenine. N-methylpurine-DNA-glycosylase is required for excision of these lesions. Recently, the 3-methyladenine-DNA-glycosylase gene of Arabidopsis thaliana was cloned and shown to be involved only in repair of 3-methyladenine. In BY-2 tobacco cells, we showed an enzymatic activity which excised both 3-methyladenine and 7-methylguanine from methylated DNA.
Kraszewska
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Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. elzbietak@ibbrain.ibb.waw.pl
Dobrzańska
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Tudek
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D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000187:drug effects; D045647:DNA Glycosylases; D019175:DNA Methylation; D006147:Guanine / Q000031:analogs & derivatives* / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000633:toxicity; D008770:Methylnitrosourea / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D009699:N-Glycosyl Hydrolases / Q000235:genetics / Q000378:metabolism; D010947:Plants, Toxic; D014026:Nicotiana / Q000166:cytology*
D009153:Mutagens; C025946:3-methyladenine; D006147:Guanine; C008450:7-methylguanine; D008770:Methylnitrosourea; D004247:DNA; D008741:Methyl Methanesulfonate; C021565:3-methyladenine-DNA glycosylase; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D000225:Adenine