Metabolism and disposition of 1,4,7,8-tetrachlorodibenzo-p-dioxin in rats.
Metabolism studies of 1,4,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a relatively nontoxic dioxin congener, were undertaken to gain a better understanding of mammalian metabolism of dioxins without the problems associated with the use of the most toxic congener, 2,3,7,8-TCDD. 14C-1,4,7,8-TCDD was dosed to conventional and bile-cannulated rats at a level of 8 mg/kg. The 14C was excreted almost entirely in 72 hours with the major routes of excretion feces and bile. Metabolites were identified from the feces, bile, and urine by GC-MS or negative ion FAB MS and 1H NMR. The two major fecal metabolites were hydroxylated tetra- and triCDDs. Glucuronide and sulfate conjugates of these hydroxyl metabolites were found in the urine and bile. Minor metabolites included dichlorocatechol, dihydroxylated tetra- and triCDDs, and conjugates of these compounds.
Huwe
J K
JK
USDA Biosciences Research Laboratory, Fargo, ND 58105, USA.
Feil
V J
VJ
Larsen
G L
GL
Wiener
C
C
D000818:Animals; D001822:Body Burden; D004785:Environmental Pollutants / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D005243:Feces / Q000737:chemistry; D006207:Half-Life; D008297:Male; D000072317:Polychlorinated Dibenzodioxins / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley
D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins