title: A simple and reliable method for hybridization of homothallic wine strains of saccharomyces cerevisiae
abstract: A procedure was developed for the hybridization and improvement of homothallic industrial wine yeasts. Killer cycloheximide-sensitive strains were crossed with killer-sensitive cycloheximide-resistant strains to get killer cycloheximide-resistant hybrids, thereby enabling hybrid selection and identification. This procedure also allows backcrossing of spore colonies from the hybrids with parental strains.
authors.affiliation: Departamento de Microbiologia, Universidad de Extremadura, 06071 Badajoz, Spain.
authors.initials: M
authors.lastname: Ramirez
authors.initials: F
authors.lastname: Perez
authors.initials: JA
authors.lastname: Regodon