Nonaqueous capillary electrophoresis with laser induced fluorescence detection.
This paper describes the use of nonaqueous capillary electrophoresis (NACE) with laser induced fluorescence (LIF) to improve detection sensitivity. The nonaqueous medium is conducive to lower detection limits due to the minimization of quenching effects. The nonaqueous solvent, N-methylformamide, produced the best detection limit with a 2-fold enhancement using NACE-LIF as compared to aqueous CE. N-methylformamide also gave the best fluorescent signal enhancement (6-fold) among the five nonaqueous solvents tested using steady-state fluorescence. The extent (or degree) of enhancement in fluorescence intensity seems to be related to the viscosity and/or polarity of the solvent.
Ward
V L
VL
Department of Chemistry, North Carolina State University, Raleigh 27695, USA.
Khaledi
M G
MG
D002138:Calibration; D019075:Electrophoresis, Capillary / Q000379:methods*; D005453:Fluorescence; D005559:Formamides; D007834:Lasers; D009281:Naphthalenes; D012680:Sensitivity and Specificity; D012997:Solvents
D005559:Formamides; D009281:Naphthalenes; D012997:Solvents; C046465:8-amino-1,3,6-naphthalenetrisulfonic acid; C002950:methylformamide