The preparation of specific sorbents with polyclonal antibodies as a ligand for purification of human antithrombin III.
Antithrombin III (AT III) is a serine protease inhibitor active against thrombin, factor X and factor VII. Major hematolytic abnormalities such as disseminated intravascular closing, coagulative vein inflammation, embolism in lungs or brain etc. frequently occur when the level of AT III is low. As a drug AT III is separated from blood preparations by bioselective sorption on sorbents containing heparine as a complementary ligand interacting with AT III molecules. The present paper describes the preparation procedures and the properties of sorbents with chemically bonded AT III antigen. The chromatographic ability of the prepared sorbents to separate AT III from human plasma are discussed in relation to the bonding procedure which was used for AT III antigen immobilization.
Rogalski
J
J
Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland.
Dawidowicz
A L
AL
Wiater
A
A
Gibuła
K
K
Winiarczyk
S
S
D000818:Animals; D000906:Antibodies / Q000097:blood / Q000302:isolation & purification; D000918:Antibody Specificity; D000990:Antithrombin III / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008024:Ligands; D011817:Rabbits
D000906:Antibodies; D007074:Immunoglobulin G; D008024:Ligands; D000990:Antithrombin III