title: Cyc2p is required for maintaining ionic stability and efficient cytochrome c import and mitochondrial function in Saccharomyces cerevisiae.
abstract: A previous study demonstrated that. Cyc2p from Saccharomyces cerevisiae is a mitochondrial protein and that cyc2 mutants contained only approximately 20% of the normal levels of cytochrome c due to a partial deficiency in mitochondrial import of apo-cytochrome c. We report herein that deletion of the entire gene results in defective mitochondrial function, as revealed by diminished growth on media containing nonfermentable carbon sources. This defect is exacerbated in hyper-ionic KCl media and at higher incubation temperatures, but is suppressed on media containing sorbitol, a non-ionic compound. We suggest that Cyc2p serves to maintain the osmotic stability of mitochondria, and its defect is exacerbated by KCl.
authors.affiliation: Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Pearce
authors.forename: T S
authors.initials: TS
authors.lastname: Cardillo
authors.forename: F
authors.initials: F
authors.lastname: Sherman
mesh_terms: D001692:Biological Transport; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D003574:Cytochrome c Group / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins; D009994:Osmolar Concentration; D017354:Point Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: C083336:CYC2 protein, S cerevisiae; D002352:Carrier Proteins; D003574:Cytochrome c Group; D005656:Fungal Proteins; D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins