title: Additive effects of hyperinsulinemia and ischemia on myocardial GLUT1 and GLUT4 translocation in vivo.
abstract: BACKGROUND
  Myocardial ischemia increases glucose uptake through the translocation of GLUT1 and GLUT4 from an intracellular compartment to the sarcolemma. The present study was performed to determine whether hyperinsulinemia causes translocation of myocardial GLUT1 as well as GLUT4 in vivo and whether there are additive effects of insulin and ischemia on GLUT1 and GLUT4 translocation. METHODS ADN RESULTS: Myocardial glucose uptake and transporter distribution were assessed by arteriovenous measurements, cell fractionation, and immunofluorescence. In fasted anesthetized dogs, hyperinsulinemia increased myocardial glucose extraction 3-fold (P<0.01) and the sarcolemmal content of GLUT4 by 90% and GLUT1 by 50% (P<0.05 for both) compared with saline infusion. In subsequent experiments, glucose uptake and transporter distribution were determined in ischemic and nonischemic regions of hearts from hyperinsulinemic animals during regional myocardial ischemia. Glucose uptake was 50% greater in the ischemic region (P<0.05). This was associated with a 20% increase in sarcolemmal GLUT1 and a 60% increase in sarcolemmal GLUT4 contents in the ischemic region (P<0.05 for both).
  
  
  CONCLUSIONS
  Insulin stimulates myocardial glucose utilization through translocation of GLUT1 as well as GLUT4. Insulin and ischemia have additive effects to increase in vivo glucose utilization and augment glucose transporter translocation. We conclude that recruitment of both GLUT1 and GLUT4 contributes to increased myocardial glucose uptake during moderate reductions in coronary blood flow under insulin-stimulated conditions.
authors.affiliation: Section of Cardiovascular Medicine, Department of Cellular and Molecular Physiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: R R
authors.initials: RR
authors.lastname: Russell
authors.forename: R
authors.initials: R
authors.lastname: Yin
authors.forename: M J
authors.initials: MJ
authors.lastname: Caplan
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.forename: J
authors.initials: J
authors.lastname: Ren
authors.forename: G I
authors.initials: GI
authors.lastname: Shulman
authors.forename: A J
authors.initials: AJ
authors.lastname: Sinusas
authors.forename: L H
authors.initials: LH
authors.lastname: Young
mesh_terms: D000818:Animals; D001692:Biological Transport; D004285:Dogs; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D006946:Hyperinsulinism / Q000378:metabolism*; D007328:Insulin / Q000502:physiology; D008297:Male; D009004:Monosaccharide Transport Proteins / Q000378:metabolism*; D009124:Muscle Proteins; D017202:Myocardial Ischemia / Q000378:metabolism*
chemical_list: D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007328:Insulin; D009004:Monosaccharide Transport Proteins; D009124:Muscle Proteins