[Tissue engineering study on repairment of injured nerve gap in rat].
The combined substances composed of chitosan gel and rough abstract contained schwanns cell (RSC) plus extra nerve growth factor (NGF) were filled in silicone chambers to bridge a 10 mm nerve gap in rat. The RSC were created by removing autonerve's epineurium and perineurium. The very same other silicone chambers with only NGF saline solution were used as control. Electrophysiologic recording and transverse section imaging analysis at 12 weeks postoperatively showed better result in the experimental group, compared with the control group. The research suggests a new tissue engineering method for repairing nerve gap.
Wu
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Dept. of Orthopedic Surgery, Changzheng Hospital Affiliated to Second Military Medical University, Shanghai.
Zhao
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D000818:Animals; D001698:Biomedical Engineering; D002686:Chitin / Q000031:analogs & derivatives* / Q000627:therapeutic use; D048271:Chitosan; D008297:Male; D009414:Nerve Growth Factors / Q000627:therapeutic use*; D009416:Nerve Regeneration; D051381:Rats; D017208:Rats, Wistar; D012583:Schwann Cells; D012584:Sciatic Nerve / Q000502:physiology / Q000601:surgery*
D009414:Nerve Growth Factors; D002686:Chitin; D048271:Chitosan