Note: identification of Lactobacillus delbrueckii subspecies bulgaricus and subspecies lactis dairy isolates by amplified rDNA restriction analysis.
Amplification and restriction analysis of the 16S rRNA gene (PCR-ARDRA) was used to identify Lactobacillus delbrueckii dairy isolates and to assign them to its three subspecies (delbrueckii, lactis and bulgaricus). PCR-ARDRA allowed confirmation of predictions from the DNA database regarding the restriction mapping of the 16S sequence to be verified in practice. PCR-ARDRA was shown to be a reliable and rapid method for identifying Lact. delbrueckii isolates at the subspecies level and for differentiating this species from Lact. helveticus and Lact. acidophilus.
Giraffa
G
G
Istituto Sperimentale Lattiero Caseario, Lodi, Italy. ilclodi@telware.telware.it
De Vecchi
P
P
Rossetti
L
L
D015139:Blotting, Southern; D004269:DNA, Bacterial / Q000032:analysis*; D003611:Dairy Products / Q000382:microbiology; D020459:Genes, rRNA; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S