Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage.
The present study describes three subsets of bone marrow-derived Lin CD43+B220(low) (B220(low)) progenitor cells which represent distinct stages in hematopoietic development. These populations differ in their expression of CD24 and ckit and the occurrence of IgH gene rearrangement. B220(low) CD24-ckit(hi) progenitors have their IgH loci in germ-line configuration and are multipotent since they can give rise to B cells, T cells and myeloid cells. B220lowckit(hi) cells which have acquired CD24 expression have retained IgH loci in germ-line configuration and the ability to generate B cells, however, they have lost the ability to generate T cells and myeloid cells. Thus acquisition of CD24 by B220(low) cells occurs concurrently with the transition from a multipotent to a lineage-restricted progenitor population. B220(low)CD24+ cells expressing low levels of ckit are also lineage restricted, giving rise to only B cells and have begun D-J(H) rearrangement, implying that initiation of IgH rearrangement coincides with the down-regulation of ckit expression.
Hunte
B E
BE
Department of Immunobiology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto 94304, USA.
Capone
M
M
Zlotnik
A
A
Rennick
D
D
Moore
T A
TA
D000818:Animals; D015703:Antigens, CD / Q000032:analysis*; D001402:B-Lymphocytes / Q000502:physiology*; D051927:CD24 Antigen; D015321:Gene Rearrangement; D005803:Genes, Immunoglobulin; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology; D017493:Leukocyte Common Antigens / Q000032:analysis*; D051917:Leukosialin; D008562:Membrane Glycoproteins; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis*; D012795:Sialoglycoproteins / Q000032:analysis*
D015703:Antigens, CD; D051927:CD24 Antigen; C497890:Cd24a protein, mouse; D051917:Leukosialin; D008562:Membrane Glycoproteins; D012795:Sialoglycoproteins; C497863:Spn protein, mouse; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens