title: Histone-like TAFs are essential for transcription in vivo.
abstract: In yeast, the TBP-associated factors (TAFs) Taf17, Taf60, and Taf61(68) resemble histones H3, H4, and H2B, respectively. To analyze their roles in vivo, conditional alleles were isolated by mutagenizing their histone homology domains. Conditional alleles of TAF17 or TAF60 can be specifically suppressed by overexpression of any of the other histone-like TAFs. This and other genetic evidence supports the model of a histone octamer-like structure within TFIID. Shifting strains carrying the conditional TAF alleles to non-permissive conditions results in degradation of TFIID components and the rapid loss of mRNA production. Therefore, in contrast to previous studies in yeast that found only limited roles for TAFs in transcription, we find that the histone-like TAFs are generally required for in vivo transcription.
authors.affiliation: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: B
authors.initials: B
authors.lastname: Michel
authors.forename: P
authors.initials: P
authors.lastname: Komarnitsky
authors.forename: S
authors.initials: S
authors.lastname: Buratowski
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000429:Alcohol Oxidoreductases; D000483:Alleles; D000595:Amino Acid Sequence; D000619:Aminohydrolases; D002455:Cell Division; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics; D006657:Histones / Q000235:genetics; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes; D016296:Mutagenesis; D010641:Phenotype; D011755:Pyrophosphatases; D012319:RNA Polymerase II / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D013489:Suppression, Genetic / Q000235:genetics; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D035362:Transcription Factor TFIID; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D019473:Transcription Factors, TFII / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000429:Alcohol Oxidoreductases; D000619:Aminohydrolases; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D006657:Histones; D009097:Multienzyme Complexes; D011755:Pyrophosphatases; D012319:RNA Polymerase II; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D035381:TATA-Binding Protein Associated Factors; D015534:Trans-Activators; D035362:Transcription Factor TFIID; D014157:Transcription Factors; D019473:Transcription Factors, TFII; C090575:ADA2 protein, S cerevisiae; C106162:HFI1 protein, S cerevisiae; C490039:TAF6 protein, S cerevisiae; C116141:TAF9 protein, S cerevisiae; C092116:HIS4 protein, S cerevisiae; C491114:TAF12 protein, S cerevisiae