SCL binds the human homologue of DRG in vivo.
A suspected oncoprotein, human development regulated GTP-binding protein (DRG) has never been identified though homologues were found in mouse, Xenopus, Drosophila, yeast and Halobacteria. During a search for SCL binding partners using the yeast 2-hybrid system, we isolated two independent cDNA clones (clone L51 and clone V3) of the human DRG homologue from human fetal liver and human thymus cDNA libraries. Only one amino acid difference was found between human and mouse DRG proteins. Although a human DRG has been previously deposited in the SWISS-PROT Database, we believe that we have cloned the bona fide human DRG based on the highly conserved primary amino acid structure between our cloned human homologue and the mouse DRG.
Zhao
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Departments of Pediatrics and Molecular Medicine, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA.
Aplan
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D000595:Amino Acid Sequence; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D003001:Cloning, Molecular; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D011518:Proto-Oncogene Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D029867:Xenopus Proteins
D051792:Basic Helix-Loop-Helix Proteins; D004268:DNA-Binding Proteins; D019204:GTP-Binding Proteins; D011518:Proto-Oncogene Proteins; D000073942:T-Cell Acute Lymphocytic Leukemia Protein 1; D014157:Transcription Factors; D029867:Xenopus Proteins; C489365:TAL1 protein, Xenopus; C489366:Tal1 protein, mouse; C067143:TAL1 protein, human; C078418:developmentally regulated GTP-binding protein