Application of lipids and plasmid design for gene delivery to mammalian cells.
Cationic lipids are widely used for in vitro gene transfer due to their efficiency. The major challenges for the improvement of in vivo cationic lipid-mediated gene delivery reside in the design of more biocompatible lipoplexes mimicking viral-mediated gene delivery and in understanding the fate of the lipoplexes within the cells.
Scherman
D
D
UMR 133 CNRS/Rhône-Poulenc Rorer, Vitry-sur-Seine, France.
Bessodes
M
M
Cameron
B
B
Herscovici
J
J
Hofland
H
H
Pitard
B
B
Soubrier
F
F
Wils
P
P
Crouzet
J
J
D000818:Animals; D001709:Biotechnology; D002412:Cations; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D015195:Drug Design; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics*
D002412:Cations; D008055:Lipids