Shared epitope homozygosity' is strongly associated with rheumatoid arthritis in Turkey. Istanbul Rheumatology Study Group.
OBJECTIVE
Associations with HLA-DRB alleles, implicated in the aetiopathogenesis of rheumatoid arthritis (RA), are found to be different in various ethnic groups. This study aimed to investigate DRB1 alleles in RA patients in Turkey.


METHODS
The DRB region of the MHC was screened by polymerase chain reaction/sequence-specific oligonucleotide (PCR/SSO) hybridizations in 101 seropositive RA patients and compared with 101 healthy controls.


RESULTS
Significant differences were in the frequencies of DRB1*0404 (12 vs 1, P = 0.003, OR = 13.5), *0401 (19 vs 4, P = 0.001, OR = 5.6) and *0408 (5 vs 0, P = 0.06, OR = 11.6) between RA patients and controls. The shared epitope (SE) was present in 70.2% of RA patients compared to 31.6% of controls (P < 0.0001, OR = 5.1). A double dose of SE was considerably more frequent in the RA group (21 vs 1, P < 0.0001, OR = 26.5).


CONCLUSIONS
These results support the reported positive association of RA with SE in seropositive patients in Turkey, and emphasize 'SE homozygosity' as the most strongly associated genetic susceptibility marker for RA.
Saruhan-Direskeneli
G
G
I.U. Istanbul Tip Fakültesi, Capa-Istanbul, Turkey.
D000483:Alleles; D001172:Arthritis, Rheumatoid / Q000097:blood / Q000235:genetics*; D004247:DNA / Q000032:analysis; D000939:Epitopes; D005260:Female; D005838:Genotype; D006684:HLA-DR Antigens / Q000097:blood / Q000235:genetics*; D059811:HLA-DRB1 Chains; D006720:Homozygote; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D014421:Turkey
D000939:Epitopes; D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D004247:DNA