title: Effects of high oxygen concentrations on microbial biosensor signals. Hyperoxygenation by means of perfluorodecalin.
abstract: Amperometric biosensors register oxygen depletion in response to analyte catabolism, and thus are limited by the availability of dissolved oxygen. Microbial sensors containing immobilized cells of Gluconobacter oxydans were hyperoxygenated to 400% of control levels and the effects on sensor responses to glucose were determined. Oxygenated perfluorodecalin (a completely fluorinated organic substance) was as effective in hyperoxygenation as direct sparging with O2, increasing sensor base medium oxygen concentrations from 9.3 to 37 mg/l. Hyperoxygenation enhanced maximal biosensor response amplitudes, particularly at high cell loading densities. Maximal response rates were also improved, although less dramatically. Results suggest that hyperoxygenation may be a new general approach for modulating biosensor responses.
authors.affiliation: Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Reshetilov
authors.forename: D A
authors.initials: DA
authors.lastname: Efremov
authors.forename: P V
authors.initials: PV
authors.lastname: Iliasov
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
authors.forename: N I
authors.initials: NI
authors.lastname: Kukushskin
authors.forename: R V
authors.initials: RV
authors.lastname: Greene
authors.forename: T D
authors.initials: TD
authors.lastname: Leathers
mesh_terms: D016947:Acetobacteraceae; D015374:Biosensing Techniques / Q000592:standards*; D005466:Fluorocarbons; D010100:Oxygen / Q000032:analysis* / Q000378:metabolism
chemical_list: D005466:Fluorocarbons; C008805:perfluorodecalin; D010100:Oxygen