title: Moderate inhibitory effect of interleukin-10 on human neutrophil and monocyte chemotaxis in vitro.
abstract: Neutrophils and monocytes are the major classes of phagocytes that migrate from the blood stream and accumulate in inflamed tissues in response to various chemoattractants. Because IL-10 is a potent anti-inflammatory cytokine, we analyzed its in vitro effect on chemotaxis using an under agarose method. We found that, as compared to prednisolone, IL-10 alone was a modest inhibitor of C5a, fMLP and IL-8-induced neutrophil chemotaxis, and C5a-induced monocyte chemotaxis. However, GM-CSF pretreatment of the cells potentiated this inhibitory effect. Similarly, the IL-10 induced modulation of the beta2 integrin CD11b/CD18 adhesion molecule expression was only observed on GM-CSF-preactivated neutrophils and monocytes. Taken together, these results suggest that the migration and accumulation of phagocytes at infection sites would not be significantly affected by IL-10 given as an immunomodulatory therapy.
authors.affiliation: Immunology Laboratory and INSERM U. 479, X. Bichat University, Paris, France.
authors.forename: M A
authors.initials: MA
authors.lastname: Vicioso
authors.forename: J J
authors.initials: JJ
authors.lastname: Garaud
authors.forename: H
authors.initials: H
authors.lastname: Réglier-Poupet
authors.forename: A
authors.initials: A
authors.lastname: Lebeaut
authors.forename: M A
authors.initials: MA
authors.lastname: Gougerot-Pocidalo
authors.forename: S
authors.initials: S
authors.lastname: Chollet-Martin
mesh_terms: D015703:Antigens, CD / Q000097:blood / Q000235:genetics; D002634:Chemotaxis, Leukocyte / Q000187:drug effects / Q000502:physiology*; D015936:Complement C5a / Q000494:pharmacology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000494:pharmacology* / Q000502:physiology; D016209:Interleukin-8 / Q000494:pharmacology; D007700:Kinetics; D016177:Macrophage-1 Antigen / Q000097:blood / Q000235:genetics; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D011239:Prednisolone / Q000494:pharmacology; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D015703:Antigens, CD; D016209:Interleukin-8; D016177:Macrophage-1 Antigen; D011994:Recombinant Proteins; D016753:Interleukin-10; D009240:N-Formylmethionine Leucyl-Phenylalanine; D015936:Complement C5a; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D011239:Prednisolone