Identification of phosphonic acids by capillary electrophoresis-ionspray mass spectrometry.
The identification of alkylphosphonic acids in spiked tap water has been investigated by on-line capillary electrophoresis-UV spectrometry-mass spectrometry (CE-UV-MS) in negative-ion mode. The 5 mM sorbic acid-ammonia electrolyte (pH 6.5) allows simultaneous indirect UV and MS (ionspray) detection. Several parameters (electrolyte pH, make-up chemical composition and make-up flow-rate) have been optimized and 5 mg/l limit of detection has been reached for these analytes in selected ion monitoring MS detection. MS-MS detection has also been investigated to reach a low detection limit (100 micrograms/l) for alkyl alkylphosphonic acids in spiked tap water. The mass spectra of these compounds exhibit a very abundant negative ion, [M-H]- (MS) with characteristic fragmentation (MS-MS) of acids and monoesters. Quantitative analysis achieved with CE-UV shows good correlation coefficients and allows accurate quantification of the analytes.
Mercier
J P
JP
Université d'Orleans, Institut de Chimie Organique et Analytique, CNRS UPRES-A, France.
Chaimbault
P
P
Morin
P
P
Dreux
M
M
Tambuté
A
A
D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D063065:Organophosphonates / Q000032:analysis*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D014881:Water Supply / Q000032:analysis*
D063065:Organophosphonates