Quantitative assay for epinephrine in dental anesthetic solutions by capillary electrophoresis.
A simple and robust method for the separation and quantification of epinephrine in dental anesthetic solutions was developed. The method allows the direct injection of high salt solutions without sample pre-treatment. Large sample plugs (5.7% of the total capillary length) are used for epinephrine determination by selective analyte focusing in capillary electrophoresis. The concentration detection limit for epinephrine is about 5.0 x 10(-7) M (90 ng ml-1) with a commercial UV detector. The separation protocol was validated in terms of its precision, linearity, accuracy and specificity.
Britz-Mckibbin
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P
Department of Chemistry, University of British Columbia, Vancouver, Canada.
Kranack
A R
AR
Paprica
A
A
Chen
D D
DD
D000766:Anesthesia, Dental; D000779:Anesthetics, Local / Q000737:chemistry*; D004837:Epinephrine / Q000032:analysis*; D006801:Humans; D014662:Vasoconstrictor Agents / Q000032:analysis*
D000779:Anesthetics, Local; D014662:Vasoconstrictor Agents; D004837:Epinephrine