title: A 1,3-beta-glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is a homologue of the yeast Bgl2p.
abstract: A 1,3-beta-glucanosyltransferase which introduces intrachain 1,6-beta linkages into 1,3-beta-glucan was isolated from the cell wall of Aspergillus fumigatus. The biochemical and molecular characterization of the A. fumigatus transferase showed it was homologous to the Saccharomyces cerevisiae and Candida albicans transferase Bgl2p. A null mutant constructed in A. fumigatus by gene replacement did not show a distinct phenotype from the parental strain. The putative function of this major cell-wall-associated protein is discussed.
authors.affiliation: Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Okada
authors.affiliation: Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
authors.forename: Lchiro
authors.initials: L
authors.lastname: Tatsuno
authors.affiliation: Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem 91010, Israel.
authors.forename: Emanuel
authors.initials: E
authors.lastname: Hanski
authors.affiliation: Department of Molecular Microbiology, Washington University School of Medicine, S t Louis, MO 63110, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Caparon
authors.affiliation: Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
authors.forename: Chihiro
authors.initials: C
authors.lastname: Sasakawa
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C117939:1,3-beta-glucanosyltransferase; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase