S-phase-dependent action of cycloheximide in relieving chromatin-mediated general transcriptional repression.
Chromatin plays a major role in the tight regulation of gene expression and in constraining inappropriate gene activity. Replication-coupled chromatin assembly ensures maintenance of these functions of chromatin during S phase of the cell cycle. Thus treatment of cells with an inhibitor of translation, such as cycloheximide (CX), would be expected to have a dramatic effect on chromatin structure and function, essentially in S phase of the cell cycle, due to uncoupled DNA replication and chromatin assembly. In this work, we confirm this hypothesis and show that CX can induce a dramatic S-phase-dependent alteration in chromatin structure that is associated with general RNA polymerase II-dependent transcriptional activation. Using two specific RNA polymerase II-transcribed genes, we confirm the above conclusion and show that CX-mediated transcriptional activation is enhanced during the DNA replication phase of the cell cycle. Moreover, we show co-operation between an inhibitor of histone deacetylase and CX in inducing gene expression, which is again S-phase-dependent. The modest effect of CX in inducing the activity of a transiently transfected promoter shows that the presence of the promoter in an endogenous chromatin context is necessary in order to observe transcriptional activation. We therefore suggest that the uncoupled DNA replication and histone synthesis that occur after CX treatment induces a general modification of chromatin structure, and propose that this general disorganization of chromatin structure is responsible for a widespread activation of RNA polymerase II-mediated gene transcription.
Cesari
M
M
Université de la Reunion, Laboratoire de Biochimie, 15 Av. René Cassin, 97489 St Denis Cedex, France.
Héliot
L
L
Meplan
C
C
Pabion
M
M
Khochbin
S
S
D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology*; D004257:DNA Polymerase II / Q000378:metabolism; D056572:Histone Deacetylase Inhibitors; D051379:Mice; D011401:Promoter Regions, Genetic; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D016196:S Phase; D014158:Transcription, Genetic; D014162:Transfection; D014407:Tumor Cells, Cultured
D002843:Chromatin; D056572:Histone Deacetylase Inhibitors; D011500:Protein Synthesis Inhibitors; D012313:RNA; D003513:Cycloheximide; D004257:DNA Polymerase II