Chromosome length polymorphism in clinical isolates of Candida parapsilosis.
Chromosome length polymorphism among 24 clinical isolates of Candida parapsilosis obtained from several human sources was analysed using pulsed-field gel electrophoresis. The isolates, from both superficial and deep infections, comprised a miscellaneous collection from the oral cavity, blood cultures, ear infections, wound secrete, a venous catheter and peritoneal dialysis fluid. Contour-clamped homogenous field electrophoresis using a hexagonal electrode was used for pulsed field gel electrophoresis. The chromosome numbers varied from seven to nine and their sizes ranged from 0.75 to 2.6 Mb. According to the electrophoretic karyotype patterns the 24 isolates could be divided into 9 profiles. However, the majority (18 isolates) fell into 3 groups comprising 7, 8 and 9 chromosomes, containing 5, 11, and 2 isolates, respectively. The remaining six isolates, all of which were from either an oral or another superficial site of isolation, could be categorized into a further six groups. These data confirm previous observations on the genomic heterogeneity of clinical isolates of C. parapsilosis, and illustrate the possible commonality in strains from related clinical habitats.
Fernando
P H
PH
Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Sri Lanka.
Samaranayake
L P
LP
D002175:Candida / Q000235:genetics* / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology*; D002179:Candidiasis, Cutaneous / Q000382:microbiology; D002180:Candidiasis, Oral / Q000382:microbiology; D015825:Chromosomes, Fungal / Q000737:chemistry*; D004271:DNA, Fungal / Q000032:analysis; D005838:Genotype; D006801:Humans; D007621:Karyotyping; D010031:Otitis / Q000382:microbiology; D011110:Polymorphism, Genetic / Q000235:genetics*
D004271:DNA, Fungal