Bioactive substances in buffy-coat-derived platelet pools stored in platelet-additive solutions.
We studied extracellular accumulation in buffy-coat-derived platelet pools suspended in platelet additive solutions (PAS). 20 platelet pools were prepared from buffy coats of 60 donors and stored in either PAS I (n = 10) or PAS II (n = 10). Samples were drawn from the donors to determine the normal range of histamine, plasminogen activator inhibitor 1 (PAI-1), myeloperoxidase (MPO) and interleukin (IL)-6. After preparation samples were collected on days 0, 5 and 7 from the 20 platelet pools respectively, to determine extracellular accumulation of the same substances. Extracellular histamine, PAI-1 and MPO were detected in significant levels (P<0.0001) immediately after preparation of the platelet pools. Only the extracellular level of PAI-1 increased significantly during storage from day 0 to day 7 (P<0.01). After preparation and storage to day 7 IL-6 was found in only three of the 20 platelet pools. Even though platelets were prepared by the buffy-coat method, to produce a low leucocyte count and thereby low cytokine (IL-6) content, there was still significant contamination by other bioactive substances.
Edvardsen
L
L
Surgical Immunology Laboratory, Hvidovre University Hospital, Denmark.
Taaning
E
E
Mynster
T
T
Hvolris
J
J
Drachman
O
O
Nielsen
H J
HJ
D001792:Blood Platelets / Q000378:metabolism*; D001793:Blood Preservation; D001800:Blood Specimen Collection / Q000379:methods; D002469:Cell Separation / Q000379:methods; D006632:Histamine / Q000097:blood; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D009195:Peroxidase / Q000097:blood; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism; D010976:Platelet Count
D015850:Interleukin-6; D017395:Plasminogen Activator Inhibitor 1; D006632:Histamine; D009195:Peroxidase