Molecular cloning of bottle-nosed dolphin (Tursiops truncatus) MHC class I cDNA.
Using polymerase chain reaction, bottle-nosed dolphin (Tursiops truncatus) major histocompatibility complex (DoLA) class I alpha chain cDNA was cloned and sequenced. Predicted amino acid sequences of DoLA class I alpha chain have cystein residues for intradomain disulfide bond formation and N-linked glycosylation sites, suggesting that DoLA class I alpha chain molecules construct alpha 1, alpha 2, and alpha 3 domain structures. Similarity of DoLA class I alpha chain cDNA to land mammal MHC class I alpha chain cDNA was 90.4% in cattle, 90.2% in horse, 89.4% in sheep, and 87.8% in human. This investigation suggests that DoLA is closely related to land mammals.
Shirai
K
K
Department of Preventive Veterinary Medicine and Animal Health, College of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Sakai
T
T
Oike
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002415:Cats; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry*; D004285:Dogs; D004289:Dolphins / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006736:Horses; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep
D018076:DNA, Complementary; D015395:Histocompatibility Antigens Class I