Changes in location and number of tartrate-resistant acid phosphatase (TRAP)-positive cells during the development of type II collagen-induced arthritis in DBA/1J mice.
The authors investigated changes in the location and number of osteoclasts and their precursors during the development of articular lesions in type II collagen-induced arthritis in mice using tartrate-resistant acid phosphatase (TRAP) staining. The limb joints were examined at 6 to 15 weeks after the second immunization. The number of TRAP-positive cells increased as the articular lesions progressed. TRAP-positive macrophage-like cells were found in the hyperplastic synovial tissue and bone marrow stroma in the early stage. In the advanced stage, in addition to many TRAP-positive osteoclasts on the bone surface, TRAP-positive macrophage-like cells were observed in the pannus apart from the bone surface in the pannus-joint junctions. The above mentioned TRAP-positive macrophage-like cells are considered to be osteoclast precursors.
Suzuki
M
M
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
Suzuki
M
M
Uetsuka
K
K
Shinozuka
J
J
Nakayama
H
H
Doi
K
K
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D001169:Arthritis, Experimental / Q000209:etiology / Q000473:pathology*; D002452:Cell Count; D003094:Collagen / Q000276:immunology*; D006651:Histocytochemistry; D007527:Isoenzymes / Q000032:analysis*; D051379:Mice; D008811:Mice, Inbred DBA; D010010:Osteoclasts / Q000201:enzymology* / Q000473:pathology; D000071681:Tartrate-Resistant Acid Phosphatase
D007527:Isoenzymes; D003094:Collagen; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase