T lymphocyte activation in bronchoalveolar lavage of patients with interstitial lung disease.
To study the role of T-lymphocytes in the patients with alveolitis due to interstitial lung disease (ILD), we have examined T cell populations in bronchoalveolar lavage (BAL) and peripheral blood (PB) of ten patients with ILD and six normal-controls via flow cytometry. The percentages of T-lymphocytes bearing the activation markers of HLA-DR (p < 0.01) and CD25 (p < 0.05) were significantly higher in BAL of ILD patients. There was no correlation between T lymphocytes subtypes and pulmonary functions and diffusion capacity (p > 0.05). In PB of ILD patients had less CD4+ T lymphocytes and CD19 cells (B lymphocytes) than controls (p < 0.05). This increased T-lymphocyte activation in BAL in contrast to PB suggested to have a role in the pathogenesis of the lung involvement in ILD.
Harmancy
E
E
Department of Chest Diseases and Haematology, Osmangazi University, Medical Faculty, Eskisehir, Turkey.
Gülbas
Z
Z
Ozdemir
N
N
Elbek
O
O
Mutlu
S
S
Kolsuz
M
M
Yurdasiper
A
A
D000328:Adult; D018893:Bronchoalveolar Lavage; D002469:Cell Separation; D005434:Flow Cytometry; D005541:Forced Expiratory Volume; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D017563:Lung Diseases, Interstitial / Q000276:immunology* / Q000503:physiopathology; D008213:Lymphocyte Activation / Q000276:immunology*; D008875:Middle Aged; D015375:Receptors, Interleukin-2 / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D006684:HLA-DR Antigens; D015375:Receptors, Interleukin-2