title: Expression of a NOS-III-like protein in human astroglial cell culture.
abstract: Evidence for the presence of a type-III nitric oxide synthase-like protein (NOS-III-like protein) in astroglial cells is reported. The mRNA of a NOS-III-like protein is constitutively expressed in human astrocytoma T67 cells, taken as an astroglial model. The nucleotide sequence of the PCR product (422 bp) shares more than 99% identity with the cDNA (from 1588 to 2009) of the human endothelial nitric oxide synthase (NOS-III). The molecular mass of the astroglial NOS-III-like protein is about 140 kDa, as observed for human NOS-III. Moreover, the astroglial NOS-III-like protein is constitutively tyrosine-phosphorylated and associated with caveolin-1. The astroglial NOS-III-like protein is apparently inactive, as reported for phosphorylated human NOS-III associated with caveolin-1.
authors.affiliation: Department of Biology, University of Rome 'Tre', Viale Marconi 446, Rome, I-00146, Italy. colasant@bio.uniroma3.it
authors.forename: M
authors.initials: M
authors.lastname: Colasanti
authors.forename: T
authors.initials: T
authors.lastname: Persichini
authors.forename: C
authors.initials: C
authors.lastname: Fabrizi
authors.forename: E
authors.initials: E
authors.lastname: Cavalieri
authors.forename: G
authors.initials: G
authors.lastname: Venturini
authors.forename: P
authors.initials: P
authors.lastname: Ascenzi
authors.forename: G M
authors.initials: GM
authors.lastname: Lauro
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
mesh_terms: D001253:Astrocytes / Q000201:enzymology*; D001254:Astrocytoma / Q000201:enzymology; D051242:Caveolin 1; D022461:Caveolins; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis* / Q000235:genetics; D052250:Nitric Oxide Synthase Type III; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D008565:Membrane Proteins; D012333:RNA, Messenger; C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III