Acid phosphatase and zinc ion-dependent acid phosphatase expression in normal human liver and in Hep G2 (human hepatocellular carcinoma) cell line.
The expression of high- and low-molecular weight acid phosphatase (HMr- and LMr-AP) and zinc ion-dependent acid phosphatase (HMr-ZnAP and LMr-ZnAP) was compared in normal human liver and in Hep G2 human hepatocarcinoma cell line extracts. The investigation was carried out using Sephadex G-100 chromatography, molecular weight determination, and analysis of some distinctive biochemical characteristics and immunochemical properties. Normal human liver and Hep G2 cell lines expressed both HMr- and LMr-AP enzymes although in different proportions. HMr-ZnAP was detected only in human liver extract, while LMr-ZnAP was present only in hepatoma cell extract, indicating that they were differentially expressed in normal and transformed human liver cells.
Panara
F
F
Istituto di Anatomia Comparata, Università di Perugia, Italy.
Guiderdone
S
S
Pellegrini
M
M
Zuccaccia
C
C
Contenti
S
S
D000135:Acid Phosphatase / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular; D002458:Cell Fractionation; D002712:Chlorides / Q000494:pharmacology*; D003911:Dextrans; D004789:Enzyme Activation / Q000502:physiology; D005782:Gels; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008114:Liver Neoplasms, Experimental; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D017967:Zinc Compounds / Q000494:pharmacology*
D002712:Chlorides; D003911:Dextrans; D005782:Gels; D007202:Indicators and Reagents; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D017967:Zinc Compounds; C008644:nitrophenylphosphate; C016837:zinc chloride; C025614:sephadex; D000135:Acid Phosphatase