Kid-1 expression is high in differentiated renal proximal tubule cells and suppressed in cyst epithelia.
The cDNA coding for the transcriptional repressor protein Kid-1 was cloned in a screen for zinc finger proteins, which are regulated during renal development and after renal ischemia. Kid-1 mRNA levels increase in the course of postnatal renal development and decrease after acute renal injury caused by ischemia or administration of folic acid. We have raised a monoclonal anti-Kid-1 antibody and demonstrate that the Kid-1 protein is strongly expressed in the proximal tubule of the adult rat kidney. During nephron development, the Kid-1 protein appears after the S-shaped body stage concomitantly with the brush-border enzyme alkaline phosphatase. In two animal models of polycystic kidney disease, the expression of Kid-1 is downregulated. The loss of expression of Kid-1 in cyst wall cells correlates with the loss of alkaline phosphatase histochemical staining. Kid-1 mRNA levels are also reduced in rodent renal cell carcinomas, another condition characterized by epithelial cell dedifferentiation and increased proliferation. We propose that Kid-1 plays an important role during the differentiation of the proximal tubule.
Witzgall
R
R
Institute of Anatomy and Cell Biology I, University of Heidelberg, 69120 Heidelberg, Germany.
Obermüller
N
N
Bölitz
U
U
Calvet
J P
JP
Cowley
B D
BD
Walker
C
C
Kriz
W
W
Gretz
N
N
Bonventre
J V
JV
D000375:Aging / Q000378:metabolism; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism; D000911:Antibodies, Monoclonal; D019556:COS Cells; D002292:Carcinoma, Renal Cell / Q000378:metabolism / Q000473:pathology; D002461:Cell Line, Transformed / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007668:Kidney / Q000166:cytology / Q000378:metabolism; D007680:Kidney Neoplasms / Q000378:metabolism / Q000473:pathology; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D007690:Polycystic Kidney Diseases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors
D000911:Antibodies, Monoclonal; D004268:DNA-Binding Proteins; D012333:RNA, Messenger; D014157:Transcription Factors; C485822:Zfp354a protein, mouse; C485823:Zfp354a protein, rat