Short tandem repeat (STR) locus HUMD8S306 in a large population sample from Germany.
Applied DNA typing in medico-legal investigations, in criminalistic practice, and in paternity cases often relies on high inclusion and exclusion probabilities. For that reason, the short autosomal tandem repeat locus D8D306 was validated for forensic use and incorporated into a nonoverlapping multiplex reaction with HUMDHFRP2 and HUMCD4: The allele frequencies of D8S306 in four different regions of Germany (n = 1220 alleles) were determined for use in a population database; the allele distributions did not significantly deviate from each other. The hererozygosity of D8S306 is 83%, expected exclusion chance in stain cases is 96% (paternity cases: 69%), the lowest amount of successfully amplified DNA was 30 pg. The alleles are in Hardy-Weinberg equilibrium.
Benecke
M
M
Office of Chief Medical Examiner, Forensic Biology Department, New York, NY 10016, USA. benecke@csi.com
Knopf
M
M
Voll
W
W
Oesterreich
W
W
Jacobi
Y
Y
Edelmann
J
J
D000483:Alleles; D005554:Forensic Medicine; D005787:Gene Frequency; D005858:Germany; D006579:Heterozygote; D006801:Humans; D020080:Tandem Repeat Sequences