Role of superoxide anion on the proliferation and c-Ha-ras or p53 expression in prostate cancer cell line PC3.
The purpose of this study was to investigate the role of superoxide anion (02-*) in the regulation of p53 or c-Ha-ras expression and proliferation in the prostate cancer cell line PC3. Cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in the presence of O2-*, basic fibroblast growth factor (bFGF) or their combination. p53 or C-Ha-ras expression in the cells treated with O2-* was assayed by fluorescence in situ hybridization (FISH). The proliferation was significantly inhibited by O2-* in a concentration-dependent manner ranging from 9 to 36 micromol/l nicotinamide adenine dinucleotid (NADH) combined with 2-8 micromol/l N-methylphenazonium methyl sulfate (PMS). Enhancement of proliferation by 2 ng/ml bFGF was significantly inhibited by O2-*. Although O2-* was not able to alter c-Ha-ras gene expression, O2-* at the concentrations of 18 micromol/l NADH and 4 micromol/l PMS upregulated the expression of p53. O2-* may modulate proliferation and gene expression in PC3 cells.
Wang
Z
Z
Department of Biology, Lanzhou University, P.R. China.
Zheng
R
R
Fu
S
S
Chen
Y
Y
Duan
G
G
Qin
D
D
Liu
G
G
D000838:Anions / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D008297:Male; D015689:Oncogene Protein p21(ras) / Q000235:genetics*; D011471:Prostatic Neoplasms; D013481:Superoxides / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D000838:Anions; D016159:Tumor Suppressor Protein p53; D016222:Fibroblast Growth Factor 2; D013481:Superoxides; D015689:Oncogene Protein p21(ras)