Putative dengue virus receptors from mosquito cells.
Dengue viruses are arthropod-borne, single-stranded RNA viruses. Aëdes aegypti and Aëdes albopictus are the principal vectors. In order to understand the molecular basis of dengue virus infections we explored the biochemical identity of dengue-2 (DEN-2) virus receptors in the Aëdes albopictus-derived cell line C6/36. We show here that DEN-2 interacts with two major polypeptides of 80 and 67 kDa. Polyclonal anti-C6/36 membrane antibodies block DEN-2 binding to intact C6/36 monolayers as well as to membrane extracts. Our results strongly suggest that the identified polypeptides are part of the DEN-2 receptors.
Muñoz
M L
ML
Departamento de Genética y Biología Molecular, CINVESTAV-1PN, México D.F., Mexico. lmunoz@gene.cinvestav.mx
Cisneros
A
A
Cruz
J
J
Das
P
P
Tovar
R
R
Ortega
A
A
D000330:Aedes / Q000737:chemistry / Q000821:virology*; D000818:Animals; D000906:Antibodies / Q000276:immunology; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry; D003716:Dengue Virus / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D010455:Peptides / Q000276:immunology; D011991:Receptors, Virus / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D000906:Antibodies; D010455:Peptides; D011991:Receptors, Virus