Androgen-dependent modulation of calbindin-D28K in hypothalamic tissue during prenatal development.
We examined the influence of androgens on fetal medial basal hypothalamic and preoptic area (MBH-POA) calbindin-D28K levels (via Western analysis) by treating pregnant rats with testosterone or flutamide, (an androgen receptor blocker). MBH-POA calbindin-D28K levels were significantly decreased in flutamide-treated male fetuses, whereas, MBH-POA calbindin-D28K levels were significantly increased in testosterone-treated female fetuses compared to controls. These results suggest that MBH-POA calbindin-D28K is modulated during prenatal development by androgens.
Watson
M A
MA
Department of Zoology, Brigham Young University, Provo, UT 84602, USA.
Taylor
H
H
Lephart
E D
ED
D001003:Anal Canal / Q000196:embryology; D000726:Androgen Antagonists / Q000494:pharmacology; D000818:Animals; D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology*; D015153:Blotting, Western; D001923:Brain Chemistry / Q000187:drug effects*; D064092:Calbindin 1; D064026:Calbindins; D005260:Female; D005485:Flutamide / Q000494:pharmacology; D007033:Hypothalamus, Middle / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D011247:Pregnancy; D011301:Preoptic Area / Q000737:chemistry / Q000196:embryology* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis / Q000378:metabolism*; D012727:Sex Characteristics; D013739:Testosterone / Q000494:pharmacology*; D014566:Urogenital System / Q000196:embryology
D000726:Androgen Antagonists; D018931:Antineoplastic Agents, Hormonal; C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G; D013739:Testosterone; D005485:Flutamide