title: Determination of succinylcholine in human plasma by high-performance liquid chromatography with electrochemical detection.
abstract: An alternative HPLC method for the quantification of the depolarizing neuromuscular blocking agent succinylcholine in human plasma is described. Drug spiked plasma and patient plasma samples were extracted using a C1 solid-phase cartridge. Succinylcholine was separated on a Cyano column and quantitated using electrochemical detection at a potential of 450 mV and 750 mV. Mobile phase consisted of a mixture of phosphoric acid-acetonitrile-methanol (45:35:25) adjusted to an apparent pH of 5. Standard curves for the quantitation were linear in the range of 250-8000 ng/ml. Between-day and within-day relative standard deviations were 5.1% and 1.7%, respectively. Mean drug recovery and accuracy was 68% and 104%, respectively.
authors.affiliation: Faculté de Pharmacie, Université de Montréal, Quebec, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Gao
authors.forename: S
authors.initials: S
authors.lastname: Roy
authors.forename: F
authors.initials: F
authors.lastname: Donati
authors.forename: F
authors.initials: F
authors.lastname: Varin
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009467:Neuromuscular Depolarizing Agents / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013390:Succinylcholine / Q000097:blood*
chemical_list: D009467:Neuromuscular Depolarizing Agents; D013390:Succinylcholine