Urinary acidification and net acid excretion in adult rats treated neonatally with enalapril.
Neonatal blockade of the renin-angiotensin system in rats induces irreversible renal histological abnormalities, including papillary atrophy and an impaired urinary concentrating ability. The aim was to investigate urinary acidification and net acid excretion in adult Wistar rats treated neonatally with enalapril (10 mg . kg-1 . day-1) or vehicle from 5 to 24 days of age. Analyses were performed in both metabolic balance studies and renal clearance experiments performed under pentobarbital sodium anesthesia. There were no differences between groups in urine pH or urinary excretion rates of bicarbonate, titratable acid, or ammonium, neither during control conditions nor after chronic NH4Cl loading (assessed before and after Na2SO4 infusion). Glomerular filtration rate, maximal tubular bicarbonate reabsorption, and the urine-to-blood PCO2 gradient in alkaline urine during NaHCO3 infusion did not differ between groups. Neonatally enalapril-treated rats showed a urine concentration defect and papillary damage. In conclusion, neonatal enalapril treatment produces a differentiated abnormality in tubular function in which urine concentration is impaired but urinary acidification and net acid excretion are intact.
Guron
G
G
Department of Physiology, Institute of Physiology and Pharmacology, Goteborg University, S413-90 Goteborg, Sweden.
Marcussen
N
N
Friberg
P
P
D000367:Age Factors; D000643:Ammonium Chloride / Q000008:administration & dosage; D000804:Angiotensin II / Q000652:urine; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001639:Bicarbonates / Q000378:metabolism; D004326:Drinking; D004656:Enalapril / Q000494:pharmacology*; D005919:Glomerular Filtration Rate; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D008297:Male; D009929:Organ Size; D000644:Quaternary Ammonium Compounds / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D013431:Sulfates / Q000008:administration & dosage; D014556:Urine / Q000737:chemistry*
D000806:Angiotensin-Converting Enzyme Inhibitors; D001639:Bicarbonates; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D000643:Ammonium Chloride; C012036:sodium sulfate; D000804:Angiotensin II; D004656:Enalapril