Formulation of a protein with propellant HFA 134a for aerosol delivery.
The purpose of this study was to investigate the formulation and delivery of a protein in a pressurized metered-dose inhaler (pMDI) containing HFA 134a as the propellant for aerosol delivery. Ethanol and surfactants, including polyoxyethylene 10 oleyl ether (Brij 97), polyoxyethylene 20 oleyl ether (Brij 98), polyoxyethylene sorbitan monooleate (Tween 80) and Aerosol OT (AOT), were investigated as formulation adjuvants to improve the dose delivery characteristics of the model protein (bovine serum albumin) containing pMDI formulations. The aqueous solution of a surfactant and protein was lyophilized to obtain a solid carrier system of the protein. Readily dispersible suspensions were obtained by suspending this solid carrier system in HFA 134a with ethanol as a dispersing aid. The formulations containing Tween 80 resulted in the highest respirable fraction. This study suggested a potential formulation containing a lyophilized complex of surfactant and protein readily dispersible in HFA 134a for delivering a therapeutic protein to the respiratory tract by inhalation.
Williams
R O
RO
Division of Pharmaceutics, College of Pharmacy, University of Texas at Austin, Austin, TX 78712-1074, USA. williro@mail.utexas.edu
Liu
J
J
D000336:Aerosols; D000431:Ethanol / Q000494:pharmacology; D006845:Hydrocarbons, Fluorinated / Q000008:administration & dosage*; D009330:Nebulizers and Vaporizers; D012710:Serum Albumin, Bovine / Q000008:administration & dosage*; D012995:Solubility; D013501:Surface-Active Agents / Q000008:administration & dosage* / Q000737:chemistry; D005466:Fluorocarbons
D000336:Aerosols; D000431:Ethanol; D006845:Hydrocarbons, Fluorinated; D012710:Serum Albumin, Bovine; D013501:Surface-Active Agents; C094049:apaflurane; D005466:Fluorocarbons