Deliganded albumin as a liquid adsorbent in the treatment of burn toxemia.
A new generation of synthetic carbon adsorbents was used in production of deliganded human serum albumin preparation. Thermal effects of officinal and deliganded albumin interaction with specific chemical markers were analyzed by flow microcalorimetry. The results demonstrated a 2.5-fold increase of the complexing ability for the deliganded one. The detoxifying potentials of deliganded albumin were studied in comparison with officinal preparation in rats with burn toxemia after IIIB-IV degree thermal injury and in model experiments with blood serum of patients after severe thermal burn. The transfusion of a 5% officinal albumin solution in rats 1 h after burn trauma resulted in a decrease of serum and liver cytosols cytotoxicity 2.2 and 2.4 times, respectively, in comparison with those of burned rats. After deliganded albumin transfusion the cytotoxic activity of blood serum dropped 8.5 times and that of the liver cytosols 18.5 times. The incubation of blood serum of injured patients with equal amounts of a 5% solutions of officinal or deliganded albumin resulted in a fall of the cytotoxicity level and the growth of binding ability. A comparative analysis of detoxifying potentials of albumin preparations has unambiguously demonstrated deliganded albumin advantages.
Sakhno
L
L
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kiev. aos@public.cs.kiev.ua
Sarnatskaya
V
V
Zinovieva
M
M
Yushko
L
L
Maslenny
V
V
Kozinets
G
G
Lossitskaya
V
V
Povstyanoy
N
N
Nikolaev
V
V
D000327:Adsorption; D000418:Albumins / Q000627:therapeutic use*; D000818:Animals; D002056:Burns / Q000150:complications*; D002151:Calorimetry; D003600:Cytosol / Q000737:chemistry / Q000378:metabolism; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D007262:Infusions, Intravenous; D008024:Ligands; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D014115:Toxemia / Q000097:blood / Q000188:drug therapy* / Q000209:etiology*
D000418:Albumins; D008024:Ligands