Development of a green fluorescent protein reporter for a yeast genotoxicity biosensor.
A reporter system, constructed for a laboratory screen for new genes involved in DNA repair in the brewer's yeast Saccharomyces cerevisiae, has been developed for use in a genotoxicity biosensor. The strain produces green fluorescent protein (yEGFP) when DNA damage has occurred. yEGFP is codon optimised for yeasts. The reporter does not respond to chemicals which delay mitosis, and responds appropriately to the genetic regulation of DNA repair. Data is presented which demonstrate strain improvements appropriate to biosensor technology: improved signal to noise ratio, ease of data collection and uncomplicated material handling.
Billinton
N
N
Department of Biomolecular Sciences, UMIST, Manchester, UK.
Barker
M G
MG
Michel
C E
CE
Knight
A W
AW
Heyer
W D
WD
Goddard
N J
NJ
Fielden
P R
PR
Walmsley
R M
RM
D015374:Biosensing Techniques; D004260:DNA Repair; D004271:DNA, Fungal / Q000032:analysis; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004271:DNA, Fungal; D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins