title: Bacteroides fragilis enterotoxin cleaves the zonula adherens protein, E-cadherin.
abstract: Strains of Bacteroides fragilis associated with diarrheal disease (enterotoxigenic B. fragilis) produce a 20-kDa zinc-dependent metalloprotease toxin (B. fragilis enterotoxin; BFT) that reversibly stimulates chloride secretion and alters tight junctional function in polarized intestinal epithelial cells. BFT alters cellular morphology and physiology most potently and rapidly when placed on the basolateral membrane of epithelial cells, suggesting that the cellular substrate for BFT may be present on this membrane. Herein, we demonstrate that BFT specifically cleaves within 1 min the extracellular domain of the zonula adherens protein, E-cadherin. Cleavage of E-cadherin by BFT is ATP-independent and essential to the morphologic and physiologic activity of BFT. However, the morphologic changes occurring in response to BFT are dependent on target-cell ATP. E-cadherin is shown here to be a cellular substrate for a bacterial toxin and represents the identification of a mechanism of action, cell-surface proteolytic activity, for a bacterial toxin.
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: S
authors.initials: S
authors.lastname: Wu
authors.forename: K C
authors.initials: KC
authors.lastname: Lim
authors.forename: J
authors.initials: J
authors.lastname: Huang
authors.forename: R F
authors.initials: RF
authors.lastname: Saidi
authors.forename: C L
authors.initials: CL
authors.lastname: Sears
mesh_terms: D001441:Bacteroides fragilis / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism*; D002448:Cell Adhesion; D004768:Enterotoxins / Q000378:metabolism; D006801:Humans; D008666:Metalloendopeptidases / Q000378:metabolism*
chemical_list: D015820:Cadherins; D004768:Enterotoxins; D008666:Metalloendopeptidases; C091198:fragilysin