Influence of the anti-allergic agent, azelastine, on tumor necrosis factor-alpha (TNF-alpha) secretion from cultured mouse mast cells.
The influence of azelastine (AZ) on tumor necrosis factor-alpha (TNF-alpha) secretllon from cultured mast cells was examined. Mast cells were induced by the long-term culture of mouse (BALB/c, Male) spleen cells with interleukin-3. Mast cells collected on day 20 in culture were stimulated with 2.5 micrograms/ml of concanavalin A (Con A) in the presence of various doses of AZ for 24 hours. AZ scarcely affected TNF-alpha secretion from mast cells when low doses (0.5 and 1.0 microgram/ml) of AZ were added to cultures. However, higher doses (5.0 and 10.0 micrograms/ml) of AZ inhibited the secretion (but not production) of TNF-alpha from mast cells. These results may suggest that some of the therapeutic effects of AZ depend on its ability to reduce the secretion of TNF-alpha from mast cells.
Matsuo
S
S
Department of Clinical Hematology, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.
Takayama
S
S
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010793:Phthalazines / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D018926:Anti-Allergic Agents; D010793:Phthalazines; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; C020976:azelastine