Sequence and phylogenetic position of a class II aldolase gene in the amitochondriate protist, Giardia lamblia.
A Giardia lamblia gene, Glfba, was cloned and sequenced. This gene codes for a 324-residue-long putative class II fructose-1, 6-bisphosphate aldolase. The positions of gaps and phylogenetic analysis with maximum likelihood and maximum parsimony methods showed the sequence to be most closely related to the as-yet uncharacterized aldolases of Helicobacter pylori and Aquifex aeolicus and to the group that comprises the Calvin-cycle aldolases of photosynthetic proteobacteria and cyanobacteria. In combination with the known taxonomic and functional distribution of class I and II aldolases, the results indicate that the G. lamblia enzyme is distinct in its evolutionary history from all eukaryotic fructose-1, 6-bisphosphate aldolases studied so far.
Henze
K
K
The Rockefeller University, New York, NY 10021, USA. henzek@rockvax.rockefeller.edu
Morrison
H G
HG
Sogin
M L
ML
MüllerM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D019143:Evolution, Molecular; D005634:Fructose-Bisphosphate Aldolase / Q000145:classification / Q000235:genetics*; D017125:Genes, Protozoan; D016829:Giardia lamblia / Q000201:enzymology* / Q000235:genetics*; D016480:Helicobacter pylori / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D016054:DNA, Protozoan; D005634:Fructose-Bisphosphate Aldolase