Carboxy terminal variants of Epstein-Barr virus-encoded latent membrane protein 1 during long-term human immunodeficiency virus infection: reliable markers for individual strain identification.
To assess the frequency and molecular polymorphism of malignancy-associated latent membrane protein 1 (LMP1) variants in human immunodeficiency virus type 1 (HIV-1) infection, 94 B-lymphoblastoid cell lines spontaneously derived from peripheral blood mononuclear cells (PBMC) and 30 PBMC samples at seroconversion and later (mean, 55 months) were analyzed by longitudinal comparative sequence analysis in 8 patients progressing to non-Hodgkin's lymphoma (AIDS-NHL), 7 patients to opportunistic infections, and 2 patients with long-term asymptomatic HIV-1 infection. The sequence polymorphism in the C-terminus of LMP1 was characteristic for strains harbored by individual patients, with high fidelity for strain identification. In 14 of the 17 patients, two different but characteristic LMP1 variants were identified. At HIV seroconversion in 8 of 15 patients, a 30-bp deletion (LMP1Delta) was present. Though serial analysis revealed a shift to LMP1Delta in some individuals, statistical analysis of the cohort does not support the hypothesis that accumulation of LMP1Delta variants in PBMC accounts for their observed high incidence in AIDS-NHL.
Berger
C
C
LINK Laboratories, Cancer Center, University of Massachusetts Medical Center, Worcester, USA.
van Baarle
D
D
Kersten
M J
MJ
Klein
M R
MR
Al-Homsi
A S
AS
Dunn
B
B
McQuain
C
C
van Oers
R
R
Knecht
H
H
D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D005819:Genetic Markers; D014644:Genetic Variation; D015658:HIV Infections / Q000821:virology*; D020276:HIV Long-Term Survivors; D015497:HIV-1; D004854:Herpesvirus 4, Human / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008137:Longitudinal Studies; D016483:Lymphoma, AIDS-Related / Q000821:virology; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D014763:Viral Matrix Proteins / Q000235:genetics*
D017931:DNA Primers; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D005819:Genetic Markers; D014763:Viral Matrix Proteins