T-cell interactions with extracellular matrix proteins in periodontal disease.
Lymphoid cell infiltration of periodontal tissues undergoing pathological destruction suggests a functional interdependence between connective tissue proteins such as collagen, fibronectin and elastin and mononuclear cells, mainly T lymphocytes. The aim of the study was to assess T lymphocyte proliferation after stimulation by mAb OKT3 and then costimulation by ECM (extracellular matrix) proteins, as well as T-cell adhesion to connective tissue proteins in patients with adult periodontitis, early-onset periodontitis, chronic gingivitis and experimental gingivitis. Controls included patients with clinically healthy periodontium. Statistical analysis of results showed a significantly decreased level of T lymphocyte proliferation in response to costimulating action of ECM proteins among patients with adult periodontitis as compared to the control group. Reactivity of T-cells was much higher in experimental gingivitis than in adult periodontitis, and significantly lower in chronic gingivitis than in controls. In addition, T-cell interaction with ECM proteins was abnormal in early-onset periodontitis patients, but was not statistically significant.
Słotwińska
S M
SM
Department of Conservative Dentistry, Dental Institute, Medical University of Warsaw, Poland. Dentam1@WAW1.Comnet.PL
Górski
A
A
Wierzbicka
M
M
Dybizbański
E
E
Mrowiec
T
T
Iwanicka-Frankowska
E
E
D000328:Adult; D000368:Aged; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D016326:Extracellular Matrix Proteins / Q000276:immunology*; D005260:Female; D005891:Gingivitis / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D010518:Periodontitis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000911:Antibodies, Monoclonal; D016326:Extracellular Matrix Proteins; D008934:Mitogens