title: Effect of IciA protein on the expression of the nrd gene encoding ribonucleoside diphosphate reductase in E. coli.
abstract: The E. coli nrd operon contains the genes encoding the two subunits of ribonucleoside diphosphate reductase. We found that the IciA protein binds specifically to the AT-rich upstream region of nrd promoter. In vivo overexpression of IciA increases the expression of nrd gene by four- to five-fold, suggesting that IciA functions as a transcriptional activator for the nrd gene.
authors.affiliation: Institute for Molecular Biology and Genetics, Seoul National University, Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Han
authors.forename: H S
authors.initials: HS
authors.lastname: Kwon
authors.forename: J B
authors.initials: JB
authors.lastname: Yim
authors.forename: D S
authors.initials: DS
authors.lastname: Hwang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012262:Ribonucleoside Diphosphate Reductase / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D029968:Escherichia coli Proteins; C068863:argP protein, E coli; D012262:Ribonucleoside Diphosphate Reductase