Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA.
Recently, a type-specific ELISA using equine herpesvirus type 1 (EHV-1) and type 4 (EHV-4) glycoprotein Gs (gGs) was developed by Crabb and Studdert [1993]. To investigate the dissemination of EHV-1 and -4 among horses in Japan, we applied their ELISA as suitable for discriminating between EHV-1 and -4 infections serologically. Type-specificity of the ELISA was confirmed by using paired sera of infected horses with either EHV-1 or -4. Application of the ELISA to sera collected before and after the winter season of 1995-1996 from 80 racehorses revealed that 30 horses showed significant antibody responses against EHV-1 and 9 against EHV-4, respectively. The results indicated that this ELISA using paired sera is useful for a diagnosis and an epizootiological study on EHV-1 and -4 infections.
Yasunaga
S
S
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
Maeda
K
K
Matsumura
T
T
Kai
K
K
Iwata
H
H
Inoue
T
T
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005334:Fever / Q000150:complications / Q000662:veterinary; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D004861:Herpesvirus 1, Equid / Q000276:immunology; D006734:Horse Diseases / Q000175:diagnosis* / Q000453:epidemiology; D006736:Horses; D007564:Japan / Q000453:epidemiology; D008297:Male; D018140:Varicellovirus / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology
D000914:Antibodies, Viral; D014759:Viral Envelope Proteins; C083103:glycoprotein G, Equid herpesvirus 1; C076629:glycoprotein G, Equid herpesvirus 4