A human monoclonal antibody against HLA-Cw1 and a human monoclonal antibody against an HLA-A locus determinant derived from a single uniparous female.
Two human monoclonal antibodies (HuMAbs) with widely different HLA specificities were raised from a uniparous HLA-seropositive female. Screening against a large panel of serologically HLA-typed lymphocytes in the complement-dependent cytotoxicity test showed that one of these HuMAbs, VP6G3, was specific for HLA-Cw1, thereby constituting the first HuMAb against an HLA-C locus product. The second HuMAb, VP5G3, was directed against an HLA-A-encoded determinant shared by HLA-A11, -A25, -A26 and -A66. The epitopes responsible for binding were determined by comparing the aminoacid sequences and were pinpointed to the 6K/9F combination for HuMAb VP6G3, and 163R with a critical contribution of aminoacids present at positions 166/167 for HuMAb VP5G3.
Mulder
A
A
Leiden University Medical Center, Department of Immunohaematology and Bloodbank, The Netherlands. mulder@rullf2.medfac.leidenuniv.nl
Kardol
M J
MJ
Uit het Broek
C M
CM
Tanke-Visser
J
J
Young
N T
NT
Claas
F H
FH
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D001402:B-Lymphocytes; D002144:Callithrix; D002460:Cell Line; D018604:Epitope Mapping; D005260:Female; D005312:Fetal Blood / Q000276:immunology; D015234:HLA-A Antigens / Q000235:genetics / Q000276:immunology*; D015236:HLA-C Antigens / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D016056:Immunodominant Epitopes / Q000276:immunology; D010298:Parity
D000911:Antibodies, Monoclonal; D015234:HLA-A Antigens; D015236:HLA-C Antigens; C047431:HLA-Cw1 antigen; D016056:Immunodominant Epitopes