Experimental murine Trypanosoma congolense infections. II. Role of splenic adherent CD3+Thy1.2+ TCR-alpha beta- gamma delta- CD4+8- and CD3+Thy1.2+ TCR-alpha beta- gamma delta- CD4-8- cells in the production of IL-4, IL-10, and IFN-gamma and in trypanosome-elicited immunosuppression.
Trypanosome-induced suppression of T and B cell responses to parasite-related and -unrelated Ags is considered a major mechanism of evasion of the host's immune defenses by the parasite. Reduced T and B cell responses have been attributed to suppressor T cells, suppressor macrophages, or both. We have previously shown that endogenously produced IL-10 and IFN-gamma mediate the suppression of T cell responses in Trypanosoma congolense-infected mice. Here, we show for the first time that splenic CD3+ Thy1.2+ alphabeta- gammadelta- CD4+ 8- and CD3+ Thy1.2+ alphabeta- gammadetla- CD4- 8- cells that copurify with plastic-, nylon wool-, or Sephadex G-10-adherent cell populations, in synergy with adherent Thy1.2- cells, are the major producers of IL-4, IL-10, and IFN-gamma in T. congolense-infected mice. We further demonstrate the involvement of these cells in the suppression of T cell proliferative responses to mitogen and in B cell responses to a parasite-unrelated Ag.
Uzonna
J E
JE
Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Kaushik
R S
RS
Zhang
Y
Y
Gordon
J R
JR
Tabel
H
H
D000818:Animals; D000941:Antigens; D000954:Antigens, Surface; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D001789:Blood Group Antigens / Q000276:immunology; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D002448:Cell Adhesion / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D003911:Dextrans; D005260:Female; D005782:Gels; D007108:Immune Tolerance; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000378:metabolism; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000378:metabolism; D015847:Interleukin-4 / Q000096:biosynthesis* / Q000378:metabolism; D037181:Lectins, C-Type; D008211:Lymphocyte Cooperation; D008212:Lymphocyte Depletion; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000276:immunology; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009757:Nylons; D010969:Plastics; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D012756:Sheep; D013154:Spleen / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D018800:Thy-1 Antigens; D014348:Trypanosoma congolense / Q000276:immunology*; D014353:Trypanosomiasis, African / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D000954:Antigens, Surface; D001789:Blood Group Antigens; D017252:CD3 Complex; D015704:CD4 Antigens; D016827:CD8 Antigens; D003911:Dextrans; D005782:Gels; D037181:Lectins, C-Type; D008934:Mitogens; D055651:NK Cell Lectin-Like Receptor Subfamily B; D009757:Nylons; D010969:Plastics; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta; D018800:Thy-1 Antigens; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma; C025614:sephadex