Incorporation of the acetylcholine receptor dimer from Torpedo californica in a peptide supported lipid membrane investigated by surface plasmon and fluorescence spectroscopy.
The dimer species (M(r) 580,000) of the nicotinic acetylcholine receptor, isolated from the electric organ of Torpedo californica, was incorporated into a thiopeptide supported lipid bilayer. The incorporation was achieved by fusion of liposomes with reconstituted receptor onto a gold-supported thiopeptide lipid monolayer. Surface plasmon resonance spectroscopy (SPS) was used to monitor in real time the fusion process as well as the specific binding of the antagonist alpha-bungarotoxin. A recently developed extension of SPS offering enhanced sensitivity and specificity, surface plasmon fluorescence spectroscopy (SPFS), was then used to monitor subsequent binding of the monoclonal WF6 and polyclonal antibody, respectively. The latter was fluorescence labeled with Cy5. The different binding assays indicate the successful incorporation of the receptor in the lipid bilayer.
Schmidt
E K
EK
Max Planck Institute for Polymer Research, Mainz, Germany.
Liebermann
T
T
Kreiter
M
M
Jonczyk
A
A
Naumann
R
R
Offenhäusser
A
A
Neumann
E
E
Kukol
A
A
Maelicke
A
A
Knoll
W
W
D000818:Animals; D004557:Electric Organ / Q000378:metabolism; D008051:Lipid Bilayers; D008567:Membranes, Artificial; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance; D014101:Torpedo / Q000378:metabolism*
D008051:Lipid Bilayers; D008567:Membranes, Artificial; D011978:Receptors, Nicotinic