Effects of nucleic acid inhibitors on the development of Angiostrongylus costaricensis in vitro.
Nucleic acid biosynthesis of Angiostrongylus costaricensis was examined with various inhibitors; aminopterin (inhibitor of purine and pyrimidine de novo biosynthetic pathways), 8-azaguanine (specific inhibitor of purine salvage pathway) and PALA (specific inhibitor of pyrimidine de novo biosynthetic pathway) were applied in in vitro culture developing from the third stage larvae to young adult in chemically defined medium. It was suggested that A. costaricensis possessed functional purine and pyrimidine de novo biosynthetic pathways and also that they could utilize exogenous sources of purines and pyrimidines by salvage pathways for their development.
Hata
H
H
Department of Parasitology, Chiba University School of Medicine, Japan.
Niimura
M
M
Yano
A
A
D000630:Aminopterin / Q000494:pharmacology; D000801:Angiostrongylus / Q000187:drug effects / Q000254:growth & development*; D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001375:Azaguanine / Q000494:pharmacology; D006131:Growth Inhibitors / Q000494:pharmacology; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology*; D009696:Nucleic Acids / Q000096:biosynthesis*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000494:pharmacology
D006131:Growth Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D009696:Nucleic Acids; D001224:Aspartic Acid; C013195:sparfosic acid; D000630:Aminopterin; D010746:Phosphonoacetic Acid; D001375:Azaguanine