The general stress sigma factor sigmaS of Escherichia coli is induced during diauxic shift from glucose to lactose.
The general stress sigma factor sigmaS (RpoS) of Escherichia coli is strongly induced in response to glucose starvation. This increase in the cellular sigmaS level is due to stabilization of sigmaS, which under non-stress conditions is subject to rapid proteolysis. In the present study, it is demonstrated that sigmaS is also induced during the diauxic shift from glucose to lactose, i.e., under conditions of glucose exhaustion in the presence of another, less-preferred carbon source that eventually gets utilized. This sigmaS induction, which is due to stabilization, is transient and precedes the induction of beta-galactosidase. In parallel, sigmaS-dependent genes are transiently activated, as was shown here for osmY. Although sigmaS can mediate transcription of lacZ in vitro, sigmaS does not contribute to the induction of beta-galactosidase during the diauxic lag phase. Rather, the induction of sigmaS and the general stress response during the diauxic shift plays the role of a rapidly activated emergency system, which is shut off again as soon as the cells are able to cope with the stress situation by utilizing a more specific and more economical system.
Fischer
D
D
Department of Biology, University of Konstanz, 78457 Konstanz, Germany.
Teich
A
A
Neubauer
P
P
Hengge-Aronis
R
R
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D005947:Glucose / Q000378:metabolism*; D007763:Lac Operon; D007785:Lactose / Q000378:metabolism*; D012808:Sigma Factor / Q000096:biosynthesis*; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D012808:Sigma Factor; C069567:sigma factor KatF protein, Bacteria; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose