title: Susceptibility to the biological effects of polyaromatic hydrocarbons is influenced by genes of the major histocompatibility complex.
abstract: Polyaromatic hydrocarbons are ubiquitous environmental chemicals that are important mutagens and carcinogens. The purpose of this study was to determine whether genes within the major histocompatibility complex (MHC) influence their biological activities. Cell-mediated immunity to dimethylbenz(a)anthracene (DMBA) was investigated in congenic strains of mice. On three different backgrounds, H-2(k) and H-2(a) haplotype mice developed significantly greater contact-hypersensitivity responses to DMBA than H-2(b), H-2(d), and H-2(s) mice. In B10.A(R1) mice, which are Kk and Id, a vigorous contact-hypersensitivity response was present, indicating that the response was governed by class I, rather than class II, MHC genes. C3H/HeN (H-2(k)) and C3H.SW (H-2(s)) strains were also compared for the development of skin tumors and the persistence of DMBA-DNA adducts. When subjected to a DMBA initiation, phorbol 12-tetradecanoate 13-acetate (TPA)-promotion skin-tumorigenesis protocol, C3H/HeN mice, (which develop cell-mediated immunity to DMBA) were found to have significantly fewer tumors than C3H.SW mice (a strain that failed to develop a cell-mediated immune response to DMBA). DMBA-DNA adducts were removed more rapidly in C3H/HeN than in C3H.SW mice. The results indicate that genes within the MHC play an important role in several of the biological activities of carcinogenic polyaromatic hydrocarbons. The observations are consistent with the hypothesis that cell-mediated immunity to chemical carcinogens serves to protect individuals by removing mutant cells before they can evolve into clinically apparent neoplasms.
authors.affiliation: Department of Dermatology and the Skin Diseases Research Center, Case Western Reserve University, Cleveland, OH 44106, USA. celmets@uab.edu
authors.forename: C A
authors.initials: CA
authors.lastname: Elmets
authors.forename: M
authors.initials: M
authors.lastname: Athar
authors.forename: K A
authors.initials: KA
authors.lastname: Tubesing
authors.forename: D
authors.initials: D
authors.lastname: Rothaupt
authors.forename: H
authors.initials: H
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Mukhtar
mesh_terms: D015127:9,10-Dimethyl-1,2-benzanthracene / Q000276:immunology* / Q000633:toxicity; D000818:Animals; D002273:Carcinogens / Q000633:toxicity; D005805:Genes, MHC Class I; D005802:Genes, MHC Class II; D007111:Immunity, Cellular / Q000235:genetics*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D011083:Polycyclic Compounds / Q000276:immunology / Q000633:toxicity
chemical_list: D002273:Carcinogens; D011083:Polycyclic Compounds; D015127:9,10-Dimethyl-1,2-benzanthracene