Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning.
Two members of a placental alkaline phosphatase (PLAP) family, PLAP and PLAP-like or germ cell alkaline phosphatase, are aberrantly expressed in tumors of ecotropic origin. To characterize alkaline phosphatase induced in seminoma, alkaline phosphatase cDNA clones were isolated from a cDNA library constructed from seminoma cells and characterized by nucleotide sequence determination. Thus, isolated cDNA clones were classified into two types, germ cell alkaline phosphatase (PLAP-like) and liver/bone/kidney-type alkaline phosphatase (L/B/K AP). These results suggest that other than the PLAP family members, the expression of L/B/K AP is enhanced in seminoma and can serve as a tumor marker in seminoma.
Shigenari
A
A
Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.
Ando
A
A
Baba
T
T
Yamamoto
T
T
Katsuoka
Y
Y
Inoko
H
H
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D010920:Placenta / Q000201:enzymology; D018239:Seminoma / Q000201:enzymology*; D013736:Testicular Neoplasms / Q000201:enzymology*
D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D000469:Alkaline Phosphatase