Standardization of smooth lipopolysaccharide preparations for use in diagnostic serological tests for bovine antibody Brucella abortus.
A procedure for standardizing Brucella abortus smooth lipopolysaccharide used in diagnostic tests for brucellosis is proposed. The procedure is based on the reactivity of antigen preparations with a panel of sera with or without antibody to B. abortus using a set of parameters established with 13 antigen preparations. For each serum dilution, a mean and two standard deviations were calculated in the indirect and competitive enzyme immunoassays. If data obtained with an antigen preparation, using the same serum dilutions, falls within the range established using two standard deviations, the antigen would be considered acceptable for diagnostic use.
Nielsen
K
K
Canadian Food Inspection Agency, Animal Diseases Research Institute, Nepean, Ontario, Canada. nielsenk@em.agr.ca
Kelly
L
L
Mallory
M
M
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002003:Brucella abortus / Q000737:chemistry* / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis; D002417:Cattle; D003627:Data Interpretation, Statistical; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000592:standards*; D012015:Reference Standards
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides