title: Hidden chromosome abnormalities in a primary central nervous system lymphoma detected by multicolor spectral karyotyping.
abstract: Cytogenetic analysis provides important information for diagnosis and prognosis in some tumors. But karyotype analysis can be difficult in some cases, because metaphase chromosomes are contracted. New techniques, such as fluorescence in situ hybridization and, more recently, spectral karyotyping, or SKY, based on the hybridization of 24 fluorescently labeled chromosome painting probes, allow the detection and identification of complex chromosomal rearrangements. We report here a case of primary central nervous system lymphoma in which chromosomal rearrangements and marker chromosomes not identified by a routine cytogenetic technique were clarified by SKY. This shows the value of the SKY technique in the cytogenetic diagnosis of tumors.
authors.affiliation: Cytogenetic Oncology Laboratory, C.H.U. TIMONE, Marseille, France.
authors.forename: H
authors.initials: H
authors.lastname: Zattara-Cannoni
authors.forename: H
authors.initials: H
authors.lastname: Dufour
authors.forename: H
authors.initials: H
authors.lastname: Lepidi
authors.forename: C
authors.initials: C
authors.lastname: Chatel
authors.forename: F
authors.initials: F
authors.lastname: Grisoli
authors.forename: A M
authors.initials: AM
authors.lastname: Vagner-Capodano
mesh_terms: D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics*; D002869:Chromosome Aberrations / Q000175:diagnosis*; D025063:Chromosome Disorders; D017809:Fatal Outcome; D005260:Female; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D016393:Lymphoma, B-Cell / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics*; D013702:Temporal Lobe