title: Mucin gene expression in human embryonic and fetal intestine.
abstract: BACKGROUND
  The intestinal epithelium is covered by a continuous layer of mucus which is secreted by well differentiated epithelial cells. Disregulation of the expression of mucins has been reported to have possible implications in the neoplastic process which affects intestinal mucosae. It is well known that preneoplastic and neoplastic tissues can express fetal phenotypic characteristics.
  
  
  OBJECTIVE
  To assess whether the expression of mucin genes in the intestinal tract is linked to the stage of cellular differentiation and tissue development, by studying the expression of six mucin genes in human fetal small intestine and colon, and also adult tissues.
  
  
  METHODS
  In situ hybridisation was used to study mRNA expression of MUC2, MUC3, MUC4, MUC5B, MUC5AC, and MUC6 in 32 human embryos and fetuses (6.5-27 weeks gestation). Normal adult mucosae were used as controls.
  
  
  RESULTS
  Three mucin genes, MUC2, MUC4, and MUC5AC, were differently expressed in fetal intestine compared with expression in normal adults.
  
  
  CONCLUSIONS
  These differences in mucin gene expression suggest a possible regulatory role for these products in intestinal epithelial cell differentiation.
authors.affiliation: Unité 377 INSERM and Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital C Huriez, 59045 Lille Cedex, France.
authors.forename: M P
authors.initials: MP
authors.lastname: Buisine
authors.forename: L
authors.initials: L
authors.lastname: Devisme
authors.forename: T C
authors.initials: TC
authors.lastname: Savidge
authors.forename: C
authors.initials: C
authors.lastname: Gespach
authors.forename: B
authors.initials: B
authors.lastname: Gosselin
authors.forename: N
authors.initials: N
authors.lastname: Porchet
authors.forename: J P
authors.initials: JP
authors.lastname: Aubert
mesh_terms: D000328:Adult; D002454:Cell Differentiation; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D004847:Epithelial Cells / Q000166:cytology; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation; D005865:Gestational Age; D006801:Humans; D017403:In Situ Hybridization; D007421:Intestine, Small / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D055271:Mucin 5AC; D055262:Mucin-2; D055263:Mucin-3; D055264:Mucin-4; D055257:Mucin-5B; D009077:Mucins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: C524625:MUC2 protein, human; C524658:MUC4 protein, human; C524678:MUC5AC protein, human; C524578:MUC5B protein, human; D055271:Mucin 5AC; D055262:Mucin-2; D055263:Mucin-3; D055264:Mucin-4; D055257:Mucin-5B; D009077:Mucins; D012333:RNA, Messenger