title: Comparative study of the venoms of three subspecies of Lachesis muta (bushmaster) from Brazil, Colombia and Costa Rica.
abstract: A comparative study was performed on the pharmacology and biochemistry of venoms from three subspecies of Lachesis muta (L. m. stenophrys, L. m. muta and L. m. rhombeata) from Brazil, Colombia and Costa Rica. All venoms induced lethal, hemorrhagic, edema-forming, myotoxic, coagulant and defibrinating effects, showing also proteolytic and indirect hemolytic activities. The venoms of L. m. stenophrys from Costa Rica and L. m. muta from Cascalheira, Brazil, had the highest lethal and hemorrhagic activities and the venom of L. m. rhombeata showed the highest coagulant activity, whereas no significant differences were observed in myotoxic and edema-forming activities at most of the time intervals studied. In addition, venoms showed similar electrophoretic patterns on SDS-polyacrylamide gel electrophoresis. In conclusion, despite quantitative differences in toxic and enzymatic activities, together with subtle variations in electrophoretic patterns, our results indicate that experimental envenomation by these venoms induce a qualitatively similar pathophysiological profile.
authors.affiliation: Facultad de Medicina, Proyecto de Ofidismo, Universidad de Antioquia, Medellín, Colombia.
authors.forename: R
authors.initials: R
authors.lastname: Otero
authors.forename: M F
authors.initials: MF
authors.lastname: Furtado
authors.forename: C
authors.initials: C
authors.lastname: Gonçalves
authors.forename: V
authors.initials: V
authors.lastname: Núñez
authors.forename: M E
authors.initials: ME
authors.lastname: García
authors.forename: R G
authors.initials: RG
authors.lastname: Osorio
authors.forename: M
authors.initials: M
authors.lastname: Romero
authors.forename: J M
authors.initials: JM
authors.lastname: Gutiérrez
mesh_terms: D000818:Animals; D001938:Brazil; D003105:Colombia; D003364:Costa Rica; D004487:Edema / Q000139:chemically induced; D015180:Electrophoresis, Gel, Two-Dimensional; D005342:Fibrinolysis / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects; D006470:Hemorrhage / Q000139:chemically induced; D007263:Infusions, Parenteral; D051379:Mice; D012910:Snake Venoms / Q000737:chemistry / Q000201:enzymology / Q000633:toxicity*; D013997:Time Factors; D017819:Viperidae / Q000502:physiology*
chemical_list: D012910:Snake Venoms