title: Parallel production of oligonucleotide arrays using membranes and reagent jet printing.
abstract: Here, we present a low-cost method to produce compact arrays using microporous materials and reagent jetting. Oligonucleotides are immobilized on membrane sheets as a series of lines. The membrane sheet is then rolled and bound, and the spiral structure is cut like a "jelly roll" to produce identical arrays. The spiral arrays behave much like larger formats using membranes, and hybridization detection can be accomplished using standard signal-generation mechanisms. The method is particularly useful for producing identical arrays from pre-synthesized oligonucleotides.
authors.affiliation: Barclay College, Haviland, KS, USA.
authors.forename: D I
authors.initials: DI
authors.lastname: Stimpson
authors.forename: P W
authors.initials: PW
authors.lastname: Cooley
authors.forename: S M
authors.initials: SM
authors.lastname: Knepper
authors.forename: D B
authors.initials: DB
authors.lastname: Wallace
mesh_terms: D004867:Equipment Design; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D015345:Oligonucleotide Probes
chemical_list: D015345:Oligonucleotide Probes