title: A new method for reliable and efficient reconstruction of 3-dimensional images from autoradiographs of brain sections.
abstract: Valuable information on metabolism and function of distinct brain regions can be extracted from autoradiographs of 2D brain sections, e.g. after labelling with the non-metabolisable sugar derivate [14C]-2-fluoro-deoxyglucose (2-FDG). For a more complete comprehension of the data and for a comparison with information obtained by modern functional imaging techniques it is essential to have a reliable and efficient method for the 3D reconstruction of autoradiographs of serial sections. This paper describes a new method for the alignment of 2D 2-FDG images, that combines two established algorithms, i.e. principal axes alignment followed by consistent matrix transformation. The power and efficiency of this new 2-step method is compared to those of various previously described procedures.
authors.affiliation: Leibniz Institute for Neurobiology, Magdeburg, Germany. hess@ifn-magdeburg.de
authors.forename: A
authors.initials: A
authors.lastname: Hess
authors.forename: K
authors.initials: K
authors.lastname: Lohmann
authors.forename: E D
authors.initials: ED
authors.lastname: Gundelfinger
authors.forename: H
authors.initials: H
authors.lastname: Scheich
mesh_terms: D000465:Algorithms; D000818:Animals; D001345:Autoradiography / Q000379:methods; D001921:Brain / Q000378:metabolism*; D002250:Carbon Radioisotopes / Q000493:pharmacokinetics; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005849:Gerbillinae; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D015203:Reproducibility of Results; D014018:Tissue Distribution
chemical_list: D002250:Carbon Radioisotopes; D019788:Fluorodeoxyglucose F18