Cationic phosphonolipids as nonviral gene transfer agents in the lungs of mice.
With the aim of developing new gene transfer tools for treating CF with gene therapy, we have synthesized a novel family of molecules named cationic phosphonolipids. The most efficient among them were selected by in vitro screening to compare their activities in vivo in mouse lungs. We used a reporter gene whose activity was measured cytofluorimetrically (FACS-Gal assay) and by means of a chemiluminescence technique. These tests allowed us to identify the percentage of transfected cells and to quantify total beta-galactosidase in the lungs. This enabled us to identify two molecules, significantly efficient in comparison with DNA alone: GLB73 (p = 0.0015) and GLB253 (p = 0.007). Their use resulted in a time lag between transfection and maximum efficiency: maximum efficiency was observed 4 days after transfection with GLB73, whereas it was noticeable only on day 7 with GLB253. Moreover, from toxicity studies carried out in vivo, GLB73 seems to be nontoxic. In vivo results were correlated with in vitro results obtained with CF epithelial cell lines. Consequently, GLB73 is a potential candidate for phase I clinical trials in humans.
Guillaume-Gable
C
C
Centre de Biogénétique, University, Hospital, ETSBO, Brest, France.
Floch
V
V
Mercier
B
B
Audrézet
M P
MP
Gobin
E
E
Le Bolch
G
G
Yaouanc
J J
JJ
Clément
J C
JC
des Abbayes
H
H
Leroy
J P
JP
Morin
V
V
Férec
C
C
D000818:Animals; D004337:Drug Carriers; D005434:Flow Cytometry / Q000379:methods; D018014:Gene Transfer Techniques; D008163:Luminescent Measurements; D008168:Lung / Q000473:pathology / Q000502:physiology*; D051379:Mice; D010743:Phospholipids / Q000737:chemistry* / Q000633:toxicity; D013329:Structure-Activity Relationship; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics / Q000378:metabolism
D004337:Drug Carriers; D010743:Phospholipids; C028299:phosphonolipids; D001616:beta-Galactosidase