title: Types of variation in DNA-A among isolates of East African cassava mosaic virus from Kenya, Malawi and Tanzania.
abstract: Complete nucleotide sequences of the DNA-A-like molecules of three East African cassava mosaic virus (EACMV) isolates from Kenya (-K, 2801 nt) and Malawi (-MH and -MK, both 2804 nt) were determined. These sequences were compared with that published for a Tanzanian isolate (-T, 2801 nt) and the partial sequence of a third Malawian isolate. Intergenic region sequences of all isolates, and deduced amino acid sequences of their AC1 (Rep) proteins, each formed a tightly related cluster that was distinct from the comparable components of other begomoviruses. Other complementary-sense genes (AC2, AC3, AC4) differed between EACMV isolates in a way consistent with the accumulation of point mutations. In contrast, virus-sense genes (CP, AV2) of isolates -MH and -MK differed (substantially for AV2) from those of other EACMV isolates but somewhat resembled those of tomato yellow leaf curl virus-Israel, suggesting they had been acquired by recombination with an unidentified begomovirus.
authors.affiliation: Scottish Crop Research Institute, Invergowrie, Dundee, UK.
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.forename: D J
authors.initials: DJ
authors.lastname: Robinson
authors.forename: B D
authors.initials: BD
authors.lastname: Harrison
mesh_terms: D002213:Capsid / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D014644:Genetic Variation; D016679:Genome, Viral; D007630:Kenya; D008295:Malawi; D002365:Manihot / Q000821:virology*; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013636:Tanzania
chemical_list: D004279:DNA, Viral