Two-dimensional mass spectrometry of biomolecules at the subfemtomole level.
Multiple dimensions of unique molecular structure information can now be obtained from proteins and DNA using mass spectrometry. Less than 10(-16) mol of the active major histocompatibility complex signaling peptide in a mixture of thousands can be identified. For large proteins (> 40 kDa), the high resolving power (> 10(5) and 10(-17) mol sensitivity of Fourier-transform mass spectrometry provide exact molecular weight values (+/- 1 or 2 Da) for mixture components, indicating error or modifications compared with the predicted DNA sequence. Selecting a specific molecular species, its two-dimensional spectrum indicates the part of the molecule that is modified; a three-dimensional spectrum of that fragment further isolates the modification site.
McLafferty
F W
FW
Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA.
Kelleher
N L
NL
Begley
T P
TP
Fridriksson
E K
EK
Zubarev
R A
RA
Horn
D M
DM
D019883:Alkyl and Aryl Transferases / Q000737:chemistry; D000818:Animals; D001483:Base Sequence; D002256:Carbonic Anhydrases / Q000737:chemistry; D002417:Cattle; D003574:Cytochrome c Group / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D005583:Fourier Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000295:instrumentation* / Q000379:methods; D006736:Horses; D008285:Major Histocompatibility Complex; D008970:Molecular Weight; D011487:Protein Conformation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D013831:Thiamine / Q000737:chemistry; D014452:Ubiquitins / Q000737:chemistry
D003574:Cytochrome c Group; D014452:Ubiquitins; D004247:DNA; D019883:Alkyl and Aryl Transferases; C022737:thiamin pyridinylase; D002256:Carbonic Anhydrases; D013831:Thiamine