Enhanced mitosis in cultured protoplasts: beneficial effects of oxygenated perfluorocarbon and haemoglobin.
Cell suspension-derived protoplasts of Petunia hybrida cv. Comanche were cultured for up to 10 d in (1) aqueous medium, (2) aqueous medium overlaying an oxygenated (10 mbar, 15 min) perfluorochemical liquid (perfluorodecalin; PFC), (3) aqueous medium containing 1:50 (v:v) of the commercial haemoglobin (Hb) solution (Erythrogen), (4) aqueous medium supplemented with 1:50 (v:v) of Erythrogen overlaying oxygenated PFC. Treatments 2, 3, and 4 enhanced mean mitotic division by 111%, 80% and 139% respectively, compared to controls. Both fresh-Hb and stored-Hb significantly (P < 0.05) enhanced mitotic division, although this was 45% greater with fresh-Hb compared to stored-Hb. Both oxygenated PFC and Erythrogen provide approaches for enhancing cellular oxygen supply to eukaryotic cells. Commercially, the recoverability and recycleability of PFCs make these compounds a more attractive option in comparison to Erythrogen, despite a high initial investment cost.
Anthony
P
P
Department of Life Science, University of Nottingham, U.K.
Davey
M R
MR
Power
J B
JB
Lowe
K C
KC
D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004338:Drug Combinations; D005466:Fluorocarbons / Q000494:pharmacology*; D006454:Hemoglobins / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D010100:Oxygen / Q000494:pharmacology*; D059828:Plant Cells; D011523:Protoplasts / Q000166:cytology / Q000187:drug effects*; D013997:Time Factors
D004338:Drug Combinations; D005466:Fluorocarbons; D006454:Hemoglobins; D010100:Oxygen