Enhanced human tumor cell transplantability in a new congenic immunodeficient mouse; KSN-BNX.
We introduced two mutant genes (beige; bg that induces the deficiency of natural killer (NK) activity and xid that decreases the production of immunoglobulin) into KSN nude mice with high reproductive performances. We produced KSN bg/bg(nu/nu) (KSN-bg), KSN-xid/xid(nu/nn) (KSN-xid), KSN xid/xid,bg/bg(nu/nu) (KSN-BNX) and KSN-nu/+ (KS) mice by back-cross (cross-intercross method). All strains showed as high a reproductivity rate as the parental KSN mice. KSN-xid and KSN-BNX mice had a reduced percentage of B220 positive cells in the spleens compared to KSN and KSN-bg mice, but they showed increased percentages of Thy-1 and asialo GM1 positive cells. The serum immunoglobulin concentrations of KSN-BNX were as low as KSN-xid. Both KSN-bg and KSN-BNX mice showed deficient NK activity in spleens, whereas KSN-xid mice showed an elevated NK activity. Compared to nude mice, the growth of both human tumor cell TCO-1 and BxPc-3 transplanted subcutaneously was enhanced in KSN-BNX mice. However Panc-1 cells that was rejected in nude mice was not accepted in KSN-BNX mice. Liver metastasis of human pancreatic tumor cells; Capan-1, BxPc-3 and MIAPaCa-2 were studied. No significant difference was observed in the percentage of metastasis formed mice between nude and KSN-BNX mice.
Ishigaki
Y
Y
Department of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.
Yasuda
K
K
Hashimoto
N
N
Nikaido
H
H
Nikaido
O
O
Hayakawa
J I
JI
D000818:Animals; D003433:Crosses, Genetic; D006801:Humans; D007108:Immune Tolerance; D007136:Immunoglobulins / Q000097:blood; D047908:Intracellular Signaling Peptides and Proteins; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D020297:Mice, Congenic / Q000276:immunology*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude / Q000235:genetics; D009368:Neoplasm Transplantation; D010190:Pancreatic Neoplasms / Q000276:immunology / Q000473:pathology; D011506:Proteins / Q000235:genetics; D013964:Thyroid Neoplasms / Q000276:immunology / Q000473:pathology; D014183:Transplantation, Heterologous; D014407:Tumor Cells, Cultured; D033921:Vesicular Transport Proteins
D007136:Immunoglobulins; D047908:Intracellular Signaling Peptides and Proteins; C102161:LYST protein, human; C491133:Lyst protein, mouse; D011506:Proteins; D033921:Vesicular Transport Proteins