title: Absence of Helicobacter pylori in dental plaque determined by immunoperoxidase.
abstract: BACKGROUND
  Attempts to detect Helicobacter pylori in dental plaque have given contrasting results, and the possibility of an oral-oral transmission of the infection remains unclear. In this study, a sensitive and specific immunoperoxidase method has been employed to assess the presence of H. pylori in dental plaque.
  
  
  METHODS
  Dental plaque smears from 80 patients undergoing gastroscopy were analyzed by an indirect immunoperoxidase test, employing a mixture of two monoclonal antibodies against H. pylori.
  
  
  RESULTS
  No immunostained bacteria were shown in any of the examined dental plaque samples.
  
  
  CONCLUSIONS
  H. pylori is not usually present in dental plaque, indicating that oral-oral transmission of the infection could be due to intermittent esophageal reflux only.
authors.affiliation: Clinica Odontoiatrica, Spedali Civili-Brescia, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Savoldi
authors.forename: M G
authors.initials: MG
authors.lastname: Marinone
authors.forename: R
authors.initials: R
authors.lastname: Negrini
authors.forename: D
authors.initials: D
authors.lastname: Facchinetti
authors.forename: A
authors.initials: A
authors.lastname: Lanzini
authors.forename: P L
authors.initials: PL
authors.lastname: Sapelli
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003773:Dental Plaque / Q000382:microbiology*; D016099:Endoscopy, Gastrointestinal; D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged; D009195:Peroxidase
chemical_list: D009195:Peroxidase