Molecular analysis of regulation of gene expression of the human erythroid anion exchanger (AE) 1.
The anion exchange protein AE1 is the most abundant membrane protein in human erythrocytes mediating the electroneutral chloride/bicarbonate exchange. We identified a promoter region in the 5' flanking region of the human AE1 gene which controls transcription in a cell type independent manner. In addition a second, distal promoter element mediates gene expression only in erythroid cells and in dependence upon differentiation. Within this distal promoter region we defined a 44 bp sequence containing a novel CT-rich motif with very strong promoter activity whereas a second 28 bp segment suppresses gene expression.
Philipp
S
S
Max-Planck-Institut für Biophysik, Frankfurt, Germany. stephan.philipp@med-rz.uni-sb.de
Kneussel
M
M
König
J
J
Appelhans
H
H
D017920:Antiporters / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D027963:Chloride-Bicarbonate Antiporters; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D008081:Liposomes; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D006454:Hemoglobins; D008081:Liposomes; D011993:Recombinant Fusion Proteins; D008156:Luciferases