Anti-complement activity of tiliroside from the flower buds of Magnolia fargesii.
As part of the search for anticomplementary active components from natural products, the anticomplementary properties of methanolic extracts from the flower buds of Magnoliafargesii have been investigated. Bioassay-guided chromatographic separation of the active constituents led to the isolation of compound 1, whose structure was identified by spectroscopic methods to be kaempferol 3-O-beta-D-(6"-O-coumaroyl)glucopyranoside (tiliroside). Tiliroside showed very potent anti-complement activity (IC50=5.4 x 10(-5) M) on the classical pathway of the complement system, even higher than rosmarinic acid, which is a well-known inhibitor against the complement system. On the other hand, the hydrolysates of tiliroside, kaempferol, astragalin and p-coumaric acid showed very weak activity on this system.
Jung
K Y
KY
Natural Product Biosynthesis Research Unit, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon.
Oh
S R
SR
Park
S H
SH
Lee
I S
IS
Ahn
K S
KS
Lee
J J
JJ
Lee
H K
HK
D001578:Benzopyrans / Q000302:isolation & purification* / Q000494:pharmacology*; D003169:Complement Inactivator Proteins / Q000302:isolation & purification* / Q000494:pharmacology*; D003171:Complement Pathway, Classical / Q000187:drug effects; D005419:Flavonoids; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013056:Spectrophotometry, Ultraviolet
D001578:Benzopyrans; D003169:Complement Inactivator Proteins; D005419:Flavonoids; D010936:Plant Extracts; C052083:tiliroside