The murine nuclear orphan receptor GCNF is expressed in the XY body of primary spermatocytes.
We have studied the expression of the nuclear orphan receptor GCNF (germ cell nuclear factor) on the mRNA and protein level in pubertal and adult mouse testes. We show by Northern and Western blot analyses and by in situ hybridization that GCNF is expressed in spermatocytes and round spermatids of adult mouse testis suggesting that GCNF may be a transcriptional regulator of spermatogenesis. Since the GCNF protein is accumulated in the XY body of late pachytene spermatocytes, it may be involved in transcriptional inactivation of sex chromosomes.
Bauer
U M
UM
Laboratory of Molecular Neurobiology, Institute of Physiological Chemistry and Pathobiochemistry, Johannes Gutenberg-University Medical School, Mainz, Germany.
Schneider-Hirsch
S
S
Reinhardt
S
S
Benavente
R
R
Maelicke
A
A
D000818:Animals; D004268:DNA-Binding Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D004848:Epithelium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D057136:Nuclear Receptor Subfamily 6, Group A, Member 1; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D013087:Spermatids / Q000378:metabolism / Q000473:pathology; D013090:Spermatocytes / Q000378:metabolism* / Q000473:pathology; D013737:Testis / Q000378:metabolism / Q000473:pathology; D014158:Transcription, Genetic
D004268:DNA-Binding Proteins; C539954:Nr6a1 protein, mouse; D057136:Nuclear Receptor Subfamily 6, Group A, Member 1; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear