title: Effects of environmentally relevant concentrations of 2,3,7,8-TCDD on domestic chicken immune function and CYP450 activity: F1 generation and egg injection studies.
abstract: Domestic chickens (Gallus gallus) were used as a surrogate species for wild turkey to assess risk from environmental 2,3,7,8-TCDD exposure. Lymphocyte proliferation and CYP450 induction were assessed in adults exposed via i.m. injection, in F1 14-day old hatchlings, in F1 adults (30-weeks old), and in 14-day old hatchlings exposed via yolk sac injections. Hatchlings from injected eggs exhibited a dose-response in lymphocyte proliferation, IgM titers, EROD, and PROD endpoints. Exposed adults showed a significant dose-dependent increase in CYP450 induction. F1 14-day old chicks exhibited a significant dose-dependent suppression of B-cell proliferation and induction of CYP450 enzymes. F1 adult proliferative responses exhibited B-cell suppression, that was not statistically significant. Significant sex-dependent EROD and MROD induction was also observed in F1 adults, indicating mixed-function oxidase imprinting from maternal exposure.
authors.affiliation: Department of Environmental Toxicology, Clemson University, Pendleton, SC 29670, USA.
authors.forename: M
authors.initials: M
authors.lastname: Peden-Adams
authors.forename: K
authors.initials: K
authors.lastname: Alonso
authors.forename: C
authors.initials: C
authors.lastname: Godard
authors.forename: S
authors.initials: S
authors.lastname: Skipper
authors.forename: W
authors.initials: W
authors.lastname: Mashburn
authors.forename: J
authors.initials: J
authors.lastname: Hoover
authors.forename: C
authors.initials: C
authors.lastname: Charbonneau
authors.forename: D
authors.initials: D
authors.lastname: Henshel
authors.forename: R
authors.initials: R
authors.lastname: Dickerson
mesh_terms: D000818:Animals; D002645:Chickens; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D019362:Cytochrome P-450 CYP2B1 / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000009:adverse effects*; D004790:Enzyme Induction; D016131:Lymphocyte Subsets / Q000187:drug effects*; D010063:Ovum; D000072317:Polychlorinated Dibenzodioxins / Q000009:adverse effects*
chemical_list: D004785:Environmental Pollutants; D000072317:Polychlorinated Dibenzodioxins; D019363:Cytochrome P-450 CYP1A1; D019362:Cytochrome P-450 CYP2B1