title: Nucleotide sequence and transcription map of porcine adenovirus type 3.
abstract: The complete nucleotide sequence of porcine adenovirus type 3 was determined and a transcriptional map for the genome was constructed. The size of the genome is 34094 bp in length with an unusually high G + C content (63.7%), the highest thus far reported for any adenovirus. Overall organization of the genome is similar to that for previously sequenced adenoviral DNAs, but there also were distinct differences. The late regions genes are organized into six families, instead of five as they are in human adenovirus type 2. In contrast to bovine adenovirus type 3 and ovine adenovirus, which lack virion-associated RNA genes, the nucleotide sequence analysis of the viral genome indicates that it encodes one short VA RNA species. With the exception of the fiber and a 33-kDa nonstructural protein, the predicted amino acid sequences of the open reading frames in the late regions and the E2 region and IVa2 exhibited a high level of homology, whereas the deduced amino acid sequences of ORFs in E1, E3, and E4 regions, and the pIX showed a lesser homology with the corresponding proteins of other adenoviruses. The proteins V, VII, and IX are unusually long, and the protein VII lacks the consensus protease cleavage site. Genomic and cDNA sequence analysis has identified promoters, cap sites, intron-exon boundaries, polyadenylation signals, and polyadenylation sites in the viral genome.
authors.affiliation: Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3, Canada. reddys@sask.usask.ca
authors.forename: P S
authors.initials: PS
authors.lastname: Reddy
authors.forename: N
authors.initials: N
authors.lastname: Idamakanti
authors.forename: J Y
authors.initials: JY
authors.lastname: Song
authors.forename: J B
authors.initials: JB
authors.lastname: Lee
authors.forename: B H
authors.initials: BH
authors.lastname: Hyun
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: S H
authors.initials: SH
authors.lastname: Cha
authors.forename: Y T
authors.initials: YT
authors.lastname: Bae
authors.forename: S K
authors.initials: SK
authors.lastname: Tikoo
authors.forename: L A
authors.initials: LA
authors.lastname: Babiuk
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D017500:Adenovirus E1A Proteins / Q000737:chemistry / Q000235:genetics; D017502:Adenovirus E2 Proteins / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D004279:DNA, Viral / Q000737:chemistry; D016679:Genome, Viral; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D013552:Swine / Q000821:virology*; D014158:Transcription, Genetic
chemical_list: D017500:Adenovirus E1A Proteins; D017502:Adenovirus E2 Proteins; D004279:DNA, Viral