cDNA sequence and chromosomal localization of mouse Dlgh3 gene adjacent to the BRCA1 tumor suppressor locus.
Membrane associated guanylate kinase homologues (MAGUKs) function in tumor suppression and receptor clustering pathways presumably by modulating signaling events at the interface of the membrane cytoskeleton. The p55 subclass of MAGUKs includes two novel cDNAs that were originally identified by virtue of their genomic location to human chromosome 17q12-21 where the BRCA1 tumor suppressor gene has been mapped. The predicted primary structure of the human MPP3 contains a single copy of the PDZ domain, an SH3 motif, and a carboxy-terminal guanylate kinase-like domain. Here we report the full-length coding cDNA sequence of the mouse homologue of MPP3. The translated amino acid sequence of murine Dlgh3 contains 568 amino acids that show 87% sequence identity with the human MPP3 protein. Northern blot analysis shows abundant expression of a approximately 3.0 kb transcript of Dlgh3 in mouse brain and skeletal muscle, and a relatively less abundant approximately 5.0 kb transcript in skeletal muscle, testis, kidney, and lung. Using an interspecific backcross panel, the Dlgh3 gene was mapped to a segment of mouse chromosome 11 that is conserved with human chromosome 17q12-21. The close proximity of murine Dlgh3 gene to the BRCA1 locus and the high conservation of the primary structure of human and murine proteins provide a framework for testing the role of Dlgh3 in cell proliferation pathways using the mouse as a model system.
Lin
L
L
Laboratory of Tumor Cell Biology, St. Elizabeth's Medical Center, Tufts University School of Medicine, ACH4, 736 Cambridge Street, Boston, MA 02135, USA.
Peters
L L
LL
Ciciotte
S L
SL
Chishti
A H
AH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D019398:Genes, BRCA1; D051528:Guanylate Kinases; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D009479:Neuropeptides; D009687:Nuclear Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D014157:Transcription Factors / Q000032:analysis / Q000737:chemistry / Q000235:genetics*
C116869:DLG3 protein, human; D018076:DNA, Complementary; D008565:Membrane Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D014157:Transcription Factors; C491075:Dlgh3 protein, mouse; D051528:Guanylate Kinases