Complement activation and CD59 expression in the motor facial nucleus following intracranial transection of the facial nerve in the adult rat.
Intracranial transection of the facial nerve has been shown to cause a massive neuronal cell death in the motor facial nucleus. Complement activation has been proposed to contribute to neuronal degeneration following axotomy. Using immunocytochemistry and in situ hybridization we show in the present study that there is complement activation in the facial nucleus after intracranial facial nerve transection as well as increase of the complement regulators CD59 and clusterin. We propose a neuroprotective role for the complement regulators CD59 and clusterin against homologous attack of complement to facial motor neurons.
Mattsson
P
P
Karolinska Hospital, Department of Clinical Neuroscience, Stockholm, Sweden. perm@neuro.ks.se
Morgan
B P
BP
Svensson
M
M
D000367:Age Factors; D000818:Animals; D019771:Axotomy; D018957:CD59 Antigens / Q000235:genetics* / Q000276:immunology*; D051152:Clusterin; D003167:Complement Activation / Q000276:immunology*; D003172:Complement C1 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015922:Complement C1q / Q000032:analysis / Q000235:genetics / Q000276:immunology; D003176:Complement C3 / Q000032:analysis / Q000235:genetics / Q000276:immunology; D015933:Complement C3d / Q000032:analysis / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary; D005154:Facial Nerve / Q000166:cytology* / Q000276:immunology / Q000601:surgery; D015870:Gene Expression / Q000276:immunology; D006023:Glycoproteins / Q000235:genetics; D017403:In Situ Hybridization; D018832:Molecular Chaperones; D009046:Motor Neurons / Q000737:chemistry / Q000276:immunology*; D009410:Nerve Degeneration / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley
D018957:CD59 Antigens; D051152:Clusterin; D003172:Complement C1; D003176:Complement C3; D018076:DNA, Complementary; D006023:Glycoproteins; D018832:Molecular Chaperones; D012333:RNA, Messenger; D015922:Complement C1q; D015933:Complement C3d