Drosophila coracle, a member of the protein 4.1 superfamily, has essential structural functions in the septate junctions and developmental functions in embryonic and adult epithelial cells.
Although extensively studied biochemically, members of the Protein 4. 1 superfamily have not been as well characterized genetically. Studies of coracle, a Drosophila Protein 4.1 homologue, provide an opportunity to examine the genetic functions of this gene family. coracle was originally identified as a dominant suppressor of EgfrElp, a hypermorphic form of the Drosophila Epidermal growth factor receptor gene. In this article, we present a phenotypic analysis of coracle, one of the first for a member of the Protein 4. 1 superfamily. Screens for new coracle alleles confirm the null coracle phenotype of embryonic lethality and failure in dorsal closure, and they identify additional defects in the embryonic epidermis and salivary glands. Hypomorphic coracle alleles reveal functions in many imaginal tissues. Analysis of coracle mutant cells indicates that Coracle is a necessary structural component of the septate junction required for the maintenance of the transepithelial barrier but is not necessary for apical-basal polarity, epithelial integrity, or cytoskeletal integrity. In addition, coracle phenotypes suggest a specific role in cell signaling events. Finally, complementation analysis provides information regarding the functional organization of Coracle and possibly other Protein 4.1 superfamily members. These studies provide insights into a range of in vivo functions for coracle in developing embryos and adults.
Lamb
R S
RS
Developmental, Cellular, and Molecular Biology Group, Duke University, Durham, North Carolina 27708-1000, USA.
Ward
R E
RE
Schweizer
L
L
Fehon
R G
RG
D000483:Alleles; D000818:Animals; D019521:Body Patterning; D003598:Cytoskeletal Proteins; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D004847:Epithelial Cells / Q000648:ultrastructure; D005260:Female; D017344:Genes, Insect; D005816:Genetic Complementation Test; D019476:Insect Proteins / Q000235:genetics* / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D008854:Microscopy, Electron; D009479:Neuropeptides; D010641:Phenotype; D017354:Point Mutation
D003598:Cytoskeletal Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; D009479:Neuropeptides; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1