Optimal lipofection reagent varies with the molecular modifications of the DNA.
Cationic lipid reagents differ in their cytofection efficacy with different cell types. No evidence has addressed whether the same lipid reagent is best for different DNAs in a single cell line. Immortalized avian embryonic cardiomyocytes cultured in vitro were tested with 15 cationic lipid reagents using (A) a beta-gal expression plasmid, (B) a fluorescein-tagged, phosphorothioate-modified ODN B, (C) a fluorescein-tagged, ethoxy-modified ODN C with the same nucleotide sequence as ODN B, and (D) a fluorescein-tagged, phosphorothioate-modified ODN D with a different nucleotide sequence from ODNs B and C. Cytofection was scored as percent of cells expressing beta-gal activity or showing diffuse cellular fluorescence. The best lipid reagents for the phosphorothioate-modified ODNs were ODN-specific and markedly different from the best lipid reagents for the expression plasmid or for the ethoxy-modified ODN. These results suggest that the best cationic lipid reagent for a particular cell type varies with the physical and chemical form of the DNA being transfected into the cells.
Conrad
A H
AH
Division of Biology, Kansas State University, Manhattan 66506-4901, USA.
Behlke
M A
MA
Jaffredo
T
T
Conrad
G W
GW
D000818:Animals; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism*; D008055:Lipids / Q000494:pharmacology; D009206:Myocardium / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D011784:Quail; D014162:Transfection / Q000187:drug effects; D001616:beta-Galactosidase / Q000096:biosynthesis
D008055:Lipids; D004247:DNA; D001616:beta-Galactosidase