A system for the delivery of general anesthetics and other volatile agents to the fruit-fly Drosophila melanogaster.
The system described here provides a simple method of delivering anesthetic vapor to the fruit-fly Drosophila melanogaster. This system delivers known concentrations of volatile anesthetic vapor obtained from liquid anesthetics in a continuous gas stream of pure humidified air. It controls for evaporation, and absorption of volatile agents, whilst allowing for extracellular electrophysiological recordings. Recordings were made from the fly's escape muscles, the jump tergotrochanter muscle (TTM) and the flight dorsal longitudinal muscle (DLM). The system minimizes the quantity of anesthetic used, making the use of more expensive and more conventional anesthetics cost effective and practicable. It also permits monitoring the fly's movements during anesthesia.
Walcourt
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Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892-4034, USA.
Ide
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D000042:Absorption; D000768:Anesthesia, General / Q000295:instrumentation* / Q000379:methods; D018681:Anesthetics, General / Q000008:administration & dosage*; D000818:Animals; D004331:Drosophila melanogaster / Q000502:physiology*; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D006221:Halothane / Q000494:pharmacology*; D009132:Muscles / Q000187:drug effects / Q000502:physiology; D011930:Reaction Time
D018681:Anesthetics, General; D006221:Halothane