title: Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1-->3),(1-->4)-Beta-D-glucan 4-glucanohydrolase.
abstract: Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield.
authors.affiliation: Laboratori de Bioquímica, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
authors.forename: J L
authors.initials: JL
authors.lastname: Viladot
authors.forename: B
authors.initials: B
authors.lastname: Stone
authors.forename: H
authors.initials: H
authors.lastname: Driguez
authors.forename: A
authors.initials: A
authors.lastname: Planas
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002240:Carbohydrate Sequence; D002384:Catalysis; D002480:Cellulase / Q000737:chemistry*; D006031:Glycosylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis*
chemical_list: D009844:Oligosaccharides; D002480:Cellulase