Application of the ribonuclease P (RNase P) RNA gene sequence for phylogenetic analysis of the genus Saccharomonospora.
Sequences of the RNase P RNA gene were investigated for the phylogenetic analysis of the genus Saccharomonospora. Aligned nucleotide sequences, determined from the PCR-amplified RNase P RNA gene of representative strains of the genus Saccharomonospora, displayed 94.2 +/- 1.3% interspecific variances. The intraspecific similarity value was 99.7-100% in all species tested. Saccharomonospora azurea K161T and 'Saccharomonospora caesia' K76T displayed identical RNase P RNA gene sequences in the region that was determined and Saccharomonospora sp. K180 showed sequences distinct from validly described species with a similarity value of 94.6 +/- 1.0%. The phylogenetic trees constructed by aligning the sequences either within the genus Saccharomonospora or with other Gram-positive bacteria were similar to the ones derived using sequences of the 16S rDNA gene. Advantageous features of this gene for application as a molecular phyletic marker are discussed.
Cho
M
M
Korean Collection for Type Cultures (KCTC), Korea Research Intitute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, Korea.
Yoon
J H
JH
Kim
S B
SB
Park
Y H
YH
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics*; D015373:Bacterial Typing Techniques; D001483:Base Sequence; D004722:Endoribonucleases / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012329:RNA, Bacterial / Q000235:genetics*; D016337:RNA, Catalytic / Q000235:genetics*; D043262:Ribonuclease P; D013045:Species Specificity
D012329:RNA, Bacterial; D016337:RNA, Catalytic; D004722:Endoribonucleases; D043262:Ribonuclease P