title: Nitric oxide synthase in the vestibulocochlear system of mice.
abstract: The exact distribution of nitric oxide-synthases (NOS) and the NO-target enzyme soluble guanylyl cyclase (sGC) in the cochlea and vestibular organ is an issue of current discussion. The existence of NOS-isoforms in the cochlea of the guinea pig has been described recently, while information about the vestibular system are still rare and non-satisfying. In order to gain more information, immunostaining was performed, using specific antibodies to NOS I-III and to sGC, on paraffin sections of complete temporal bones from mice. NOS III could be detected in cochlea and vestibular ganglion cells, in nerve fibres, in outer hair cells of the cochlear and in the sensory epithelium of the maculae. Also, the spiral ligament and the limbus epithelium was positive to NOS III. NOS I was found in the sensory epithelium of the maculae and cristae ampullares, outer and inner hair cells of the cochlea, in nerve fibres and in ganglion cells. In contrast to that NOS II could not be detected at all. Furthermore, a strong NOS I immunoreaction was displayed on the endosteum of the bone, while the periosteum was lacking of NOS. NOS detection was accompanied by immunoreactivity to sGC. The findings imply that NOS I and III-generated NO is involved in neurotransmission and other regulative processes in the vestibulocochlear system.
authors.affiliation: Department of Oto-Rhino-Laryngology, University of Cologne, Joseph-Stelzmannstr. 9, D-50924, Cologne, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Hess
authors.forename: W
authors.initials: W
authors.lastname: Bloch
authors.forename: S
authors.initials: S
authors.lastname: Arnhold
authors.forename: C
authors.initials: C
authors.lastname: Andressen
authors.forename: E
authors.initials: E
authors.lastname: Stennert
authors.forename: K
authors.initials: K
authors.lastname: Addicks
authors.forename: O
authors.initials: O
authors.lastname: Michel
mesh_terms: D000818:Animals; D003051:Cochlea / Q000201:enzymology*; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D009419:Nerve Tissue Proteins / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis*; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; D014722:Vestibule, Labyrinth / Q000201:enzymology*; D000159:Vestibulocochlear Nerve / Q000201:enzymology*
chemical_list: D009419:Nerve Tissue Proteins; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III; C496321:Nos1 protein, mouse; C496317:Nos2 protein, mouse; C496315:Nos3 protein, mouse