title: The influence of bovine growth hormone and growth hormone releasing factor on acetyl-CoA carboxylase and fatty acid synthase in primiparous Holstein cows.
abstract: Primiparous Holstein cows received recombinant bovine growth hormone (bGH), bovine growth hormone-releasing factor (bGRF), or no treatment from 118 to 181 +/- 1 d. Milk yield was significantly increased with no change in milk fat percentage or composition. The mRNA and protein abundance of the key lipogenic enzymes acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) were measured in the mammary gland and adipose tissue. We hypothesized that bGH and bGRF treatment would increase the mRNA and protein abundance of ACC and FAS in the mammary gland, with an associated decrease in adipose tissue. Analysis of ACC mRNA and protein abundance in the mammary gland revealed that there was no significant influence of either bGH or bGRF treatment. Analysis of FAS mRNA in mammary gland revealed that both bGH and bGRF significantly increased the abundance. However, quantitation of FAS protein in the mammary gland revealed that neither treatment resulted in increased abundance. In adipose tissue, the mRNA and protein abundance of both ACC and FAS were significantly reduced. The increased substrate required for increased milk fatty acid yield may be provided through redirection of nutrients to the mammary gland away from adipose tissue and through overall increased metabolism of the mammary gland.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
authors.forename: N S
authors.initials: NS
authors.lastname: Beswick
authors.forename: J J
authors.initials: JJ
authors.lastname: Kennelly
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis* / Q000235:genetics; D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D015152:Blotting, Northern; D015153:Blotting, Western; D002417:Cattle; D064429:Fatty Acid Synthases / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D013006:Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D013007:Growth Hormone-Releasing Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000201:enzymology*; D008892:Milk / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone; D064429:Fatty Acid Synthases; D000103:Acetyl-CoA Carboxylase