title: Sonochemicals increase the mutation frequency of V79 cells in vitro.
abstract: Phosphate buffered saline (PBS) was insonated or sham-insonated (1 MHz, 35 W/cm2, continuous wave, 30 min) in rotating (200 rpm) sterile polystyrene culture tubes. After treatment, the PBS was used immediately to suspend washed Chinese hamster V79 cells in vitro. Cells were incubated in the PBS at 37 degrees C for 15 min and then transferred to complete growth medium. Some insonation regimens also involved the inclusion of Albunex (ALX; an ultrasound microbubble contrast agent) to enhance ultrasound-induced inertial cavitation. Following exposure to the pretreated PBS and 6 d of subculture in complete medium, the cells were assayed for plating efficiencies and mutation frequencies (resistance to 6-thioguanine). X-rays (3 Gy) served as a positive control. Cells exposed to insonated PBS with or without ALX or x-rays had statistically significantly elevated mean mutation frequencies (4.37+/-0.97, 4.54+/-1.00, and 24.28+/-3.83 mutant colonies/10(6) viable cells, respectively) relative to corresponding control regimens (ultrasound sham, 2.44+/-0.56; x-ray sham, 2.96+/-0.88 mutant colonies/10(6) viable cells. The data supported the hypothesis that sonochemicals resulting from inertial cavitation have mutagenic potential.
authors.affiliation: Department of Biology, Shiga University of Medical Science, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Doida
authors.forename: K R
authors.initials: KR
authors.lastname: Marcello
authors.forename: A A
authors.initials: AA
authors.lastname: Brayman
authors.forename: C
authors.initials: C
authors.lastname: Cox
authors.forename: S
authors.initials: S
authors.lastname: Barned
authors.forename: M W
authors.initials: MW
authors.lastname: Miller
mesh_terms: D000418:Albumins / Q000633:toxicity*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D003287:Contrast Media / Q000633:toxicity*; D006224:Cricetinae; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D005347:Fibroblasts / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000528:radiation effects; D009154:Mutation / Q000187:drug effects* / Q000528:radiation effects; D014463:Ultrasonography
chemical_list: D000418:Albumins; C060687:Albunex; D003287:Contrast Media