title: Amperometric biosensors based on solid binding matrices applied in food quality monitoring.
abstract: Solid binding matrix (SBM) based composite transducers have been used for development of series of multibiosensor systems applicable in various fields. Here we present two hybrid three-channel multibiosensors for simultaneous amperometric operation in food quality control, i.e. glucose/fructose/ethanol multibiosensor, based on glucose oxidase/fructose dehydrogenase/alcohol dehydrogenase surface-modified enzyme electrodes and L-lactate/L-malate/sulfite multibiosensor, based on L-lactate dehydrogenase/L-malate dehydrogenase/sulfite oxidase surface-modified enzyme electrodes. Different parameters have been studied in order to optimize the response of the multibiosensor systems. The multibiosensor showed a good sensitivity, linear range and storage stability. The multibiosensors were used for the determination of glucose, fructose, ethanol, L-lactate, L-malate and sulfite in samples of wine, resulting in a good agreement with data certified by the supplier. Comparison of various designs, surface-modified, bulk-modified and thick-cover, of SBM based biosensors is studied on the example of fructose biosensor.
authors.affiliation: International Centre for Science and High Technology, UNIDO Area/Science Park, Trieste, Italy. stano@goblin.icc.trieste.it
authors.forename: S
authors.initials: S
authors.lastname: Miertus
authors.forename: J
authors.initials: J
authors.lastname: Katrlík
authors.forename: A
authors.initials: A
authors.lastname: Pizzariello
authors.forename: M
authors.initials: M
authors.lastname: Stred'anský
authors.forename: J
authors.initials: J
authors.lastname: Svitel
authors.forename: J
authors.initials: J
authors.lastname: Svorc
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D000431:Ethanol / Q000032:analysis; D005504:Food Analysis; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000032:analysis; D008293:Malates / Q000032:analysis
chemical_list: D008293:Malates; D005632:Fructose; D019344:Lactic Acid; D000431:Ethanol; C030298:malic acid; D005947:Glucose