Direct plasma injection for high-performance liquid chromatographic-mass spectrometric quantitation of the anxiolytic agent CP-93 393.
A direct plasma injection method has been developed for the rapid analysis of drugs in biological fluids. A new generation restricted access media column specifically designed to accommodate direct injection of plasma and other fluids is utilized for on-line HPLC-ESI-MS analysis. For rapid analysis the on-line extraction column is linked to a HPLC-ESI-MS system. Good results are obtained for the quantitation of CP-93 393 and deuterated internal standard over the range of 10-1000 ng/ml. The lower limit of detection for the assay was 58 pg injected on column. Accuracy and precision values are 9.0% or better over the entire range of the assay. In addition, more than 200 injections (100 microl) were performed per column with unattended, automated analysis.
Needham
S R
SR
Department of Drug Metabolism, Central Research Division, Pfizer Inc, Groton, CT 06340, USA.
Cole
M J
MJ
Fouda
H G
HG
D014151:Anti-Anxiety Agents / Q000097:blood*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011719:Pyrazines / Q000097:blood*; D013388:Succinimides / Q000097:blood*
D014151:Anti-Anxiety Agents; C109842:CP 93393; D011719:Pyrazines; D013388:Succinimides