title: mraY is an essential gene for cell growth in Escherichia coli.
abstract: The synthesis of the murein precursor lipid I is performed by MraY. We have shown that mraY is an essential gene for cell growth. Cells depleted of MraY first swell and then lyse. The expression of mraY DNA in vitro produces a 40-kDa polypeptide detectable by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
authors.affiliation: Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland. DBOYLE@srv0.bio.ed.ac.uk
authors.forename: D S
authors.initials: DS
authors.lastname: Boyle
authors.forename: W D
authors.initials: WD
authors.lastname: Donachie
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D002455:Cell Division / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D015870:Gene Expression; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016254:Mutagenesis, Insertional; D010641:Phenotype; D010957:Plasmids / Q000235:genetics; D014166:Transferases; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D014166:Transferases; D017855:Transferases (Other Substituted Phosphate Groups); C079803:mraY protein, Bacteria