title: Molecular cloning and expression of the rat EAAT4 glutamate transporter subtype.
abstract: Glutamate transport is a primary mechanism for the synaptic inactivation of glutamate. Excitatory amino acid transporter 4 (EAAT4) is a novel glutamate transporter with properties of a ligand-gated chloride channel that was recently cloned from human brain. Here we report the cloning of rat EAAT4 (rEAAT4) cDNA from rat cerebellum. The nucleotide sequence of rEAAT4 was 88% identical to the human sequence, and the predicted peptide was 89% identical to the human protein. The transport activity encoded by rEAAT4 has high affinity for L-glutamate. In Xenopus laevis oocytes expressing rEAAT4, L-glutamate and other transporter substrates elicited a current predominantly carried by chloride ions. Like human EAAT4, the rEAAT4 mRNA was largely restricted to cerebellar Purkinje cells; the rEAAT4 protein was localized to Purkinje cell somas and dendrites.
authors.affiliation: Department of Neurology, Johns Hopkins University, Baltimore, MD, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Lin
authors.forename: A V
authors.initials: AV
authors.lastname: Tzingounis
authors.forename: L
authors.initials: L
authors.lastname: Jin
authors.forename: A
authors.initials: A
authors.lastname: Furuta
authors.forename: M P
authors.initials: MP
authors.lastname: Kavanaugh
authors.forename: J D
authors.initials: JD
authors.lastname: Rothstein
mesh_terms: D027322:Amino Acid Transport System X-AG; D000818:Animals; D018118:Chloride Channels / Q000235:genetics* / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004558:Electric Stimulation; D004594:Electrophysiology; D050581:Excitatory Amino Acid Transporter 4; D015870:Gene Expression / Q000502:physiology; D050491:Glutamate Plasma Membrane Transport Proteins; D018698:Glutamic Acid / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D008024:Ligands; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000502:physiology; D011689:Purkinje Cells / Q000737:chemistry* / Q000502:physiology*; D051381:Rats; D017470:Receptors, Glutamate / Q000235:genetics* / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D027981:Symporters; D014982:Xenopus laevis
chemical_list: D027322:Amino Acid Transport System X-AG; D018118:Chloride Channels; D002712:Chlorides; D018076:DNA, Complementary; D050581:Excitatory Amino Acid Transporter 4; D050491:Glutamate Plasma Membrane Transport Proteins; D008024:Ligands; D017470:Receptors, Glutamate; C493389:Slc1a6 protein, rat; D027981:Symporters; D018698:Glutamic Acid