title: Developmental regulation of group III metabotropic glutamate receptors modulating adenylate cyclase activity in the avian retina.
abstract: The regulation of adenylate cyclase by neurotransmitters is observed in early development of the chick retina. In the present work we show that L-2-amine-4-phosphonobutyric acid (L-AP4), the major agonist of group III metabotropic glutamate receptors (mGluRs), inhibits the accumulation of cyclic AMP induced by forskolin in the chick retina. This effect is observed after 8 days of development (E8), is maximal from E12-E17 and decreases at the post-hatching period (PH). The inhibition is also observed in cultures of retinal cells incubated for 2-8 days. We have also investigated the interaction between group III mGluRs and other receptors coupled to adenylate cyclase in the developing retina. The inhibition by L-AP4 is partially additive with that induced by the A1 adenosine agonist Cyclohexyladenosine and is not observed when cyclic AMP levels are increased with 2-chloroadenosine or dopamine. The group II mGluR agonist trans-(1S,3R)-1-amino-cyclopentanedicarboxylic acid has an inhibitory effect only on PH retinas, indicating that group II and group III mGluRs have a differential ontogenesis in this tissue. The results show that Group III mGluRs are expressed early during chick retina development and do not interact with other receptors known to be coupled to adenylate cyclase in the developing retina.
authors.affiliation: Department of Neurobiology, Institute of Biology, Federal Fluminense University, Niterói, RJ, Brazil.
authors.forename: L F
authors.initials: LF
authors.lastname: Sampaio
authors.forename: R
authors.initials: R
authors.lastname: Paes-de-Carvalho
mesh_terms: D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo / Q000378:metabolism / Q000502:physiology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D011422:Propionates / Q000494:pharmacology; D011954:Receptors, Dopamine / Q000502:physiology; D018094:Receptors, Metabotropic Glutamate / Q000819:agonists / Q000378:metabolism / Q000502:physiology*; D018047:Receptors, Purinergic P1 / Q000502:physiology; D012160:Retina / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism
chemical_list: C114785:2-amino-4-phosphono-propinate; D000067956:Adenylyl Cyclase Inhibitors; D004791:Enzyme Inhibitors; D011422:Propionates; D011954:Receptors, Dopamine; D018094:Receptors, Metabotropic Glutamate; D018047:Receptors, Purinergic P1; D000262:Adenylyl Cyclases