title: The DrrC protein of Streptomyces peucetius, a UvrA-like protein, is a DNA-binding protein whose gene is induced by daunorubicin.
abstract: DrrC, a daunorubicin resistance protein with a strong sequence similarity to the UvrA protein involved in excision repair of DNA, is induced by daunorubicin in Streptomyces peucetius and behaves like an ATP-dependent, DNA binding protein in vitro. The refolded protein obtained from expression of the drrC gene in Escherichia coli was used to conduct gel retardation assays. DrrC bound a DNA segment containing the promoter region of a daunorubicin production gene only in the presence of ATP and daunorubicin. This result suggests that DrrC is a novel type of drug self-resistance protein with DNA binding properties like those of UvrA. Western blotting analysis with a polyclonal antiserum generated against His-tagged DrrC showed that the appearance of DrrC in S. peucetius is coincident with the onset of daunorubicin production and that the drrC gene is induced by daunorubicin. These data also showed that the DnrN and DnrI regulatory proteins are required for drrC expression. The level of DrrA, another daunorubicin resistance protein that resembles ATP-dependent bacterial antiporters, was regulated in the same way as that of DrrC.
authors.affiliation: School of Pharmacy, University of Wisconsin, Madison 53706, USA.
authors.forename: K
authors.initials: K
authors.lastname: Furuya
authors.forename: C R
authors.initials: CR
authors.lastname: Hutchinson
mesh_terms: D000251:Adenosine Triphosphatases; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D015153:Blotting, Western; D004269:DNA, Bacterial / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D003630:Daunorubicin / Q000494:pharmacology*; D004352:Drug Resistance, Microbial / Q000235:genetics; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D017510:Protein Folding; D013302:Streptomyces / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors
chemical_list: D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C092188:DnrN protein, Streptomyces peucetius; D029968:Escherichia coli Proteins; D014157:Transcription Factors; C068631:UvrA protein, E coli; D000251:Adenosine Triphosphatases; D003630:Daunorubicin