title: New spectrophotometric procedure for determining cefotaxime based on derivatization with 1,2-naphthoquinone-4-sulphonate into solid-phase extraction cartridges--application to pharmaceutical and urine samples.
abstract: Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot/Valencia, Spain.
authors.forename: L G
authors.initials: LG
authors.lastname: Martinez
authors.forename: P
authors.initials: P
authors.lastname: Campíns-Falcó
authors.forename: A
authors.initials: A
authors.lastname: Sevillano-Cabeza
authors.forename: F
authors.initials: F
authors.lastname: Bosch-Reig
mesh_terms: D000328:Adult; D002439:Cefotaxime / Q000032:analysis* / Q000652:urine; D002511:Cephalosporins / Q000032:analysis* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D002511:Cephalosporins; D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations; C011942:1,2-naphthoquinone-4-sulfonate; D002439:Cefotaxime