title: In vitro ligation of ureters and urethra modulates fetal mouse bladder explants development.
abstract: The compliance of the bladder which accommodates the holding and voiding of urine is influenced by the amount and type of collagen deposited as well as the packing and organization of collagen fiber bundles. During fetal development, the accumulation of urine within the bladder lumen is associated with the maturation of the bladder's wall. Fetal mouse bladders can undergo maturation as organ cultured explants in defined medium. Polarized light optics of Sirius red-stained sections of fetal mouse bladders in organ culture for 4 days showed that the ligation of both ureters and urethra promoted more orderly packing of collagen fiber bundles within the luminal edge of the lamina propria compared to unligated bladder explants. It is proposed that ligation causes differences in the development and organization of the collagen fiber bundles within the bladder wall. These differences are due to either increases in intravesical pressure, the accumulation of growth factors within the lumen or a combination of both.
authors.affiliation: Department of Surgery, Hershey Medical Center, Hershey, PA 17033-0850, USA.
authors.forename: A
authors.initials: A
authors.lastname: Beauboeuf
authors.forename: S
authors.initials: S
authors.lastname: Ordille
authors.forename: D R
authors.initials: DR
authors.lastname: Erickson
authors.forename: H P
authors.initials: HP
authors.lastname: Ehrlich
mesh_terms: D000818:Animals; D003094:Collagen / Q000032:analysis; D005260:Female; D008026:Ligation; D051379:Mice; D008815:Mice, Inbred Strains; D009924:Organ Culture Techniques; D011247:Pregnancy; D014513:Ureter / Q000737:chemistry / Q000166:cytology*; D014521:Urethra / Q000737:chemistry / Q000166:cytology*; D001743:Urinary Bladder / Q000737:chemistry / Q000166:cytology*
chemical_list: D003094:Collagen