title: Occupational asthma by Anisakis simplex.
abstract: BACKGROUND
  No cases of occupational asthma caused by the inhalation of antigens from Anisakis simplex have been published.
  
  
  OBJECTIVE
  The purpose of this study was to evaluate the possibility that A simplex can play a role in the asthma experienced by 2 workers when handling fish and fish flour.
  
  
  METHODS
  Skin prick and bronchial challenge tests with A simplex were performed. We also carried out measurements of specific IgE to A simplex and immunoblotting.
  
  
  RESULTS
  Both patients had strong positive skin test responses, challenge test responses, and specific IgE to A simplex. Immunoblotting showed that both patients also had IgE against several bands in the fish flour extract, suggesting contamination by Anisakis allergens.
  
  
  CONCLUSIONS
  These 2 patients provide evidence for occupational asthma caused by A simplex, based on in vivo and in vitro tests for Anisakis-specific IgE.
authors.affiliation: Servicios de Alergia y Bioquímica del Hospital Rio Hortega, Valladolid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Armentia
authors.forename: M
authors.initials: M
authors.lastname: Lombardero
authors.forename: A
authors.initials: A
authors.lastname: Callejo
authors.forename: J M
authors.initials: JM
authors.lastname: Martín Santos
authors.forename: F J
authors.initials: FJ
authors.lastname: Gil
authors.forename: J
authors.initials: J
authors.lastname: Vega
authors.forename: M L
authors.initials: ML
authors.lastname: Arranz
authors.forename: C
authors.initials: C
authors.lastname: Martínez
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology; D001249:Asthma / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D001985:Bronchial Provocation Tests; D002645:Chickens; D005394:Fish Flour / Q000009:adverse effects; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000175:diagnosis / Q000276:immunology* / Q000469:parasitology; D011201:Poultry Diseases / Q000276:immunology / Q000469:parasitology; D012882:Skin Tests
chemical_list: D000485:Allergens; D000947:Antigens, Helminth; D007073:Immunoglobulin E