Early IL-4 induction in bone marrow lymphoid precursor cells by mycobacterial lipoarabinomannan.
IL-4 is produced promptly in response to certain infections and plays a key role in the Th1/Th2 T cell dichotomy; however, the cellular source remains a matter of debate. Here we describe the induction of IL-4 in bone marrow cells of normal and RAG-/- mice by both Mycobacterium tuberculosis and its major cell wall glycolipid, lipoarabinomannan. Characterization of the cell type responsible indicated that it was distinct from the NK1+ or CD4+ T cell previously ascribed the function of rapid IL-4 secretion. Cell-sorting experiments identified CD19+/B220+ precursor cells, presumably pre-B cells that produced IL-4 constitutively and whose frequency was rapidly and markedly up-regulated by lipoarabinomannan. Thus, pathogenic mycobacteria and their glycolipids may influence hemopoiesis by rapidly inducing IL-4 secretion in the bone marrow.
Collins
H L
HL
Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany. collins@mpiib-berlin.mpg.de
Schaible
U E
UE
Kaufmann
S H
SH
D000818:Animals; D000941:Antigens / Q000032:analysis; D000942:Antigens, Bacterial / Q000378:metabolism / Q000494:pharmacology*; D000954:Antigens, Surface; D001089:Arabinose / Q000276:immunology; D016175:B-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism*; D002473:Cell Wall / Q000276:immunology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000276:immunology / Q000378:metabolism*; D016130:Immunophenotyping; D015847:Interleukin-4 / Q000096:biosynthesis*; D037181:Lectins, C-Type; D008070:Lipopolysaccharides / Q000378:metabolism / Q000494:pharmacology*; D008358:Mannose / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016513:Mice, SCID; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014376:Tuberculosis / Q000276:immunology
D000941:Antigens; D000942:Antigens, Bacterial; D000954:Antigens, Surface; D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; C050016:lipoarabinomannan; D015847:Interleukin-4; D001089:Arabinose; D008358:Mannose