Spatially restricted expression of pipe in the Drosophila egg chamber defines embryonic dorsal-ventral polarity.
Expression of pipe in the somatic tissue of the Drosophila ovary is required for the formation of embryonic dorsal-ventral polarity. pipe, which encodes an enzyme similar to the glycosaminoglycan-modifying enzyme heparan sulfate 2-O-sulfotransferase, is expressed in a spatially restricted domain of follicle cells on the ventral side of the egg chamber. Mutations that affect follicular polarity correspondingly alter the spatial pattern of pipe expression. Directed expression of pipe in otherwise pipe mutant females restores embryonic lateral and ventral pattern elements and can orient the dorsal-ventral axis of the embryo. Thus, the localized expression of pipe and the spatially restricted modification of carbohydrate chains play pivotal roles in the mechanisms that establish embryonic pattern and integrate follicular and embryonic polarity.
Sen
J
J
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Goltz
J S
JS
Stevens
L
L
Stein
D
D
D020121:5' Untranslated Regions / Q000235:genetics / Q000502:physiology; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019521:Body Patterning / Q000235:genetics*; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004527:Egg Proteins / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D015238:Sulfotransferases / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000502:physiology
D020121:5' Untranslated Regions; D029721:Drosophila Proteins; D004527:Egg Proteins; D019476:Insect Proteins; D020033:Protein Isoforms; D015238:Sulfotransferases; C106358:heparan-sulfate 2-sulfotransferase; C115847:pip protein, Drosophila