[Use of Brucellar protein antigen synthesized in Escherichia coli cells in the enzyme immunoassay].
The possibility of using brucellar protein antigens with mol. weights of 18 and 38 kD, synthesized in E.coli cells, as sensitins on polystyrene plates in the enzyme immunoassay (EIA) for the detection of antibodies in the blood sera of patients with different forms of brucellosis. The use of antigens with mol. weights of 18 and 38 kD made it possible to detect antibodies in patients with the acute form of the disease in 68.97% and 75.86% of cases, and in patients with the chronic form in 33.3% and 22.9% of cases respectively. The main advantage of using antigen with a mol. weight of 38 kD in EIA was its specificity. Antibodies to heterologous microorganisms (Yersinia enterocolitica O:9) having common antigenic determinants with Brucella were not detected. Still the titers of antibodies isolated with the use of these antigens were lower than those obtained in reactions with B.abortus 99 S-lipopolysaccharide.
Zheludkov
M M
MM
Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia.
Kulakov
Iu K
IuK
D000208:Acute Disease; D000941:Antigens / Q000276:immunology*; D000942:Antigens, Bacterial / Q000096:biosynthesis*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis*; D002908:Chronic Disease; D004926:Escherichia coli / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D008970:Molecular Weight; D015009:Yersinia Infections / Q000175:diagnosis
D000941:Antigens; D000942:Antigens, Bacterial; C012031:sensitin
Ispol'zovanie v immunofermentnom analize belkovykh antigenov brutsell, sintezirovannykh v kletkakh Escherichia coli.