Immunologic cross-reactivity between respiratory chemical sensitizers: reactive dyes and cyanuric chloride.
BACKGROUND
CyCl is a low molecular weight reactive chemical used as an intermediate in the production of plastics, herbicides, pharmaceuticals, and fiber-reactive dyes. It is a potent inducer of specific IgE antibody. The CyCl functionality is a structural component of monochlorotriazine and dichlorotriazine dyes.


OBJECTIVE
We have investigated the immunologic cross-reactivity between cyanuric chloride (CyCl) and reactive dyes and it was hypothesized that this moiety might be a dye epitope and that it might stimulate an allergic antibody response in dye-exposed workers.


METHODS
To test this hypothesis, we have used sera with IgE antibodies to CyCl and also sera from dye-exposed workers who have IgE antibodies to Procion Orange MX2R, an azo dye containing the dichlorotriazine group. As a control group we have used dye-exposed workers with IgE antibody to Remazol Black B, a diazo dye containing the vinyl sulfone-reactive group.


RESULTS
Using RAST and RAST inhibitions, we identified negligible cross-reactivity between CyCl and dichlorotriazine dye.


CONCLUSIONS
The results of this study imply that the allergenic moiety on the dye residue resides in the chromophore rather than in the common structural component of CyCl and dichlorotriazine dyes.
Jones
M
M
Department of Occupational and Environmental Medicine, Imperial College School of Medicine at the National Heart and Lung Institute, London, United Kingdom.
Graham
C
C
Taylor
A N
AN
Sarlo
K
K
Hoyle
V
V
Karol
M H
MH
D000918:Antibody Specificity; D004396:Coloring Agents; D003429:Cross Reactions / Q000276:immunology*; D006801:Humans; D009784:Occupational Diseases / Q000097:blood / Q000276:immunology; D011852:Radioallergosorbent Test; D012709:Serum Albumin / Q000737:chemistry; D014227:Triazines / Q000097:blood / Q000276:immunology
D004396:Coloring Agents; D012709:Serum Albumin; D014227:Triazines; C019309:cyanuric chloride; C041397:Procion Orange MX-2R