Round window administration of gentamicin: a new method for the study of ototoxicity of cochlear hair cells.
Damage to inner ear sensory hair cells after systemic administration of ototoxic drugs has been documented in humans and animals. Birds have the ability to regenerate new hair cells to replace those damaged by drugs or noise. Unfortunately, the systemic administration of gentamicin damages both ears in a variable fashion with potentially confounding systemic drug effects. We developed a method of direct application of gentamicin to one cochlea of hatchling chickens, allowing the other ear to serve as a within-animal control. We tested variables including the vehicle for application, location of application, dosage, and duration of gentamicin exposure. After 5 or 28 days survival, the percent length damage to the cochlea and regeneration of hair cells was evaluated using scanning electron microscopy. Controls consisted of the opposite unexposed cochlea and additional animals which received saline instead of gentamicin. Excellent damage was achieved using gentamicin-soaked Gelfoam pledgets applied to the round window membrane. The percent length damage could be varied from 15 to 100% by changing the dosage of gentamicin, with exposures as short as 30 min. No damage was observed in control animals. Regeneration of hair cells was observed in both the base and apex by 28 days survival.
Husmann
K R
KR
Department of Otolaryngology and the Smith Mental Retardation Research Center, University of Kansas Medical Center, Kansas City 66160-7380, USA.
Morgan
A S
AS
Girod
D A
DA
Durham
D
D
D000818:Animals; D002645:Chickens; D005781:Gelatin Sponge, Absorbable; D005839:Gentamicins / Q000008:administration & dosage* / Q000633:toxicity*; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000293:injuries / Q000502:physiology; D006801:Humans; D008855:Microscopy, Electron, Scanning; D014677:Pharmaceutical Vehicles; D012038:Regeneration; D012405:Round Window, Ear / Q000187:drug effects*; D013997:Time Factors
D005839:Gentamicins; D014677:Pharmaceutical Vehicles