Molecular cloning of capillary permeability-increasing enzyme-2 from Agkistrodon caliginosus (Korean viper).
The gene of capillary permeability-increasing enzyme-2 (CPI enzyme-2) was cloned from the cDNA library of Agkistrodon caliginosus and its nucleotide sequence was determined. Its sequence indicates that CPI enzyme-2 is synthesized as a pre-zymogen of 258 amino acid residues, including a putative secretory signal peptide of 18 amino acids and a proposed zymogen peptide of 6 amino acids. The amino terminal sequence deduced from the cDNA sequence was exactly consistent with that of CPI enzyme-2 except for the substitution of an amino acid (Gly27-->Ser). The open reading frame is very similar to those of plasminogen activator and thrombin-like proteases cloned from other snakes. The clone encoding CPI enzyme-2 belongs to the serine protease family. The active site of the enzyme is highly conserved at His41, Asp86 and Ser180. Its possible glycosylation sites, Asn-X-Thr/Ser, are located at amino acid residues 20-22, 55-57, 79-81 and 97-99.
Hahn
B S
BS
Natural Products Research Institute, Seoul National University, South Korea.
Baek
K
K
Kim
W S
WS
Lee
C S
CS
Chang
I L
IL
Kim
Y S
YS
D017836:Agkistrodon; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004798:Enzymes / Q000737:chemistry*; D015723:Gene Library; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics*; D014757:Viper Venoms / Q000201:enzymology / Q000235:genetics* / Q000633:toxicity
D018076:DNA, Complementary; D004798:Enzymes; D014757:Viper Venoms; D012697:Serine Endopeptidases; C085657:capillary permeability-increasing enzyme 2