Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico.
A novel extremely halophilic archaeon was isolated from the solar salterns of Cabo Rojo, Puerto Rico. The organism is very pleomorphic, motile and requires at least 8% (w/v) NaCl to grow. Polar lipid composition revealed the presence of a novel non-sulfate-containing glycolipid and the absence of the glycerol diether analogue of phosphatidylglycerosulfate. The G + C content of the DNA is 59 mol%. On the basis of 16S rRNA sequence data, the new isolate cannot be classified in one of the recognized genera, but occupies a position that is distantly related to the genus Haloferax. All these features justify the creation of a new genus and a new species for the family Halobacteriaceae, order Halobacteriales. The name Halogeometricum borinquense gen. nov., sp. nov. is proposed. The type strain is ATCC 700274T.
Montalvo-Rodríguez
R
R
Biology Department, University of Puerto Rico, Mayaguez 00680-5000.
Vreeland
R H
RH
Oren
A
A
Kessel
M
M
Betancourt
C
C
López-Garriga
J
J
D001482:Base Composition; D019641:DNA, Archaeal / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D006216:Halobacteriaceae / Q000145:classification* / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D008055:Lipids / Q000032:analysis; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011647:Puerto Rico; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D009626:Terminology as Topic; D014871:Water Microbiology
D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D008055:Lipids; D012336:RNA, Ribosomal, 16S