Uptake and metabolism of [3H]testosterone by Penicillium crustosum maintained in cultures at different pHs.
In order to determine the uptake and the metabolism of [3H]T by the mycelium of Penicillium crustosum, cultures of the fungi containing radiolabeled testosterone were developed at different pHs. Also, the metabolism of this androgen in the growth medium of the fungus was determined. Results show that the [3H]T was taken up by the mycelium at pHs 6, 7, 8 and 9. In addition, the presence of [3H]DHT in the incubation medium indicated the participation of 5 alpha-reductase. The maximal activities of this enzyme were determined at pHs 6 and 8, suggesting the presence of two isozymes: type 1 (pH 8), and type 2 (pH 6). Into the mycelium only [3H]T was identified, indicating that isozymes are produced by the fungus under testosterone stimuli.
Gutiérrez
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Department of Biological Systems, Metropolitan University-Xochimilco, Mexico, D.F.
Cabeza
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D042944:Cholestenone 5 alpha-Reductase; D003470:Culture Media / Q000737:chemistry; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D010088:Oxidoreductases / Q000145:classification / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*; D014316:Tritium / Q000378:metabolism
D003470:Culture Media; D014316:Tritium; D013739:Testosterone; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase