The effect of Lp3 enlargement on the folding and catalysis of hepatitis delta virus cis-cleaving ribozyme.
Oligonucleotides were inserted into the Lp3 region of a hepatitis delta virus (HDV) cis-cleaving ribozyme and the effect of Lp3 enlargement on ribozyme folding was examined by assaying the activity of each mutant. The location of insertion and the sequence of the inserted oligonucleotide had distinct effects on ribozyme activity, and the HDV ribozyme was capable of adopting the active structure for cis-cleavage when a pentanucleotide was inserted into the 3' portion of Lp3. Furthermore, whether the insertion mutant cis-cleaved or not, the structure of Lp3 was altered by the inserted oligonucleotide. These findings disclose that the 5' rather than the 3' portion of Lp3 is critical for ribozyme folding and catalysis.
Wu
H N
HN
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC. hnwu@gate.sinica.edu.tw
Lee
J Y
JY
Ping
Y H
YH
Wang
C N
CN
D002384:Catalysis; D003698:Hepatitis Delta Virus / Q000378:metabolism*; D016254:Mutagenesis, Insertional; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000235:genetics; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D009841:Oligonucleotides; D016337:RNA, Catalytic; D012367:RNA, Viral