Analysis of spatial and temporal characteristics of Ca2+ release in permeabilized parotid cells.
Ca2+ waves and oscillations have been observed during stimuli that generate inositol 1,4,5-trisphosphate (IP3) in several exocrine cells, including rat parotid acinar cells. Although a model has been proposed to explain the mechanism of the Ca2+ wave, there exists no direct evidence to prove the model's validity. Analysis with a permeabilized cell system provides direct information about the properties of Ca2+ stores and Ca2+ release channels. We summarize here the experimental techniques that may be used to elucidate the spatial and temporal regulation of Ca2+ in a single permeabilized cell. The future applications of these methods and the possible mechanisms of the Ca2+ wave are also discussed.
Tanimura
A
A
Dept. Dental Pharmacology, School of Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Japan. tanimura@hoku-iryo-u.ac.jp
Tojyo
Y
Y
Matsumoto
Y
Y
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism; D010306:Parotid Gland / Q000166:cytology* / Q000378:metabolism*
D002118:Calcium