title: Limitations of current diagnostic procedures for the diagnosis of Taenia solium cysticercosis in rural pigs.
abstract: The aim of the present study was to evaluate diagnostic procedures for porcine cysticercosis. Sera were obtained from 32 pigs reared in commercial farms, 47 pigs before and after experimental infection, 42 carefully necropsied rural pigs and 191 slaughtered pigs from rural communities in which the presence of the Taenia solium metacestode was assessed by tongue dissection. Sera were analyzed by ELISA to detect antibodies against T. solium antigens and to detect parasite antigens. Most sera from the necropsied rural pigs were also evaluated by the Western blot method. Antigen and antibody ELISA detection assays showed high sensitivity and specificity when applied to sera from pigs reared in commercial farms. In contrast, all methods (Ag-ELISA, Ab-ELISA assays, EITB and tongue inspection) showed lower sensitivity and specificity when applied to the generally lightly infected rurally reared pigs. The probability distribution of cysts in carcasses were also determined. These results emphasize the difficulties in detecting cysticercosis in rural pigs with low levels of cyst burdens.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico, DF. edda@servidor.unam.mx
authors.forename: E
authors.initials: E
authors.lastname: Sciutto
authors.forename: J J
authors.initials: JJ
authors.lastname: Martínez
authors.forename: N M
authors.initials: NM
authors.lastname: Villalobos
authors.forename: M
authors.initials: M
authors.lastname: Hernández
authors.forename: M V
authors.initials: MV
authors.lastname: José
authors.forename: C
authors.initials: C
authors.lastname: Beltrán
authors.forename: F
authors.initials: F
authors.lastname: Rodarte
authors.forename: I
authors.initials: I
authors.lastname: Flores
authors.forename: J R
authors.initials: JR
authors.lastname: Bobadilla
authors.forename: G
authors.initials: G
authors.lastname: Fragoso
authors.forename: M E
authors.initials: ME
authors.lastname: Parkhouse
authors.forename: L J
authors.initials: LJ
authors.lastname: Harrison
authors.forename: A S
authors.initials: AS
authors.lastname: de Aluja
mesh_terms: D000818:Animals; D000829:Animals, Domestic; D000909:Antibodies, Helminth / Q000097:blood; D003551:Cysticercosis / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*; D003552:Cysticercus; D004797:Enzyme-Linked Immunosorbent Assay; D008800:Mexico; D011237:Predictive Value of Tests; D012423:Rural Health; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology; D013621:Taenia
chemical_list: D000909:Antibodies, Helminth