Strain specific variation in IFN-gamma inducible lymphocyte adhesion to rat brain endothelial cells.
We have examined the interferon-gamma (IFN-gamma) induced increase in lymphocyte adhesion to rat brain endothelial cells (BEC) in the experimental allergic encephalomyelitis (EAE) susceptible LEW and resistant PVG strain. A significant increase in adhesion of mitogen activated lymphocytes could be demonstrated by stimulating LEW BEC with 50 U/ml IFN-gamma for 24 h. In contrast the same treatment failed to induce a significant increase in lymphocyte adhesion in the PVG strain. Flow cytometric analysis of lymphocyte integrin expression indicated a marked increase in the number of cells expressing both LFA-1 and VLA-4 following mitogen activation and was similar between the LEW and PVG strain. Depletion of LFA-1 and VLA-4 positive lymphocytes resulted in an equivalent inhibition of adhesion to BBB-EnC indicating that the adherent population was comprised predominantly of the VLA-4 + /LFA-1 + phenotype. We conclude that this strain variation in the IFN-gamma activation of BEC may be related to the disease phenotypes of these strains.
Linke
A T
AT
Department of Infection and Immunity, KCL, London, UK. adam.linke@kcl.ac.uk
Greenwood
J
J
Campbell
I
I
Luthert
P
P
Male
D K
DK
D000704:Analysis of Variance; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000276:immunology*; D001923:Brain Chemistry / Q000276:immunology; D002448:Cell Adhesion / Q000187:drug effects / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D004727:Endothelium / Q000166:cytology / Q000276:immunology; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology* / Q000494:pharmacology; D008214:Lymphocytes / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D051381:Rats; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology
D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D007371:Interferon-gamma