Carcinoembryonic antigen-related cell-cell adhesion molecule C-CAM is greatly increased in serum and urine of rats with liver diseases.
C-CAM (rat cell CAM/human CD66a) is ubiquitous and multifunctional. It is involved in intercellular adhesion, signal transduction and cell growth inhibition. Structurally, it is related to the carcinoembryonic antigen. In the present study serum, bile and urine of rats with liver diseases were analyzed for the presence of cell CAM. After bile duct ligation and during galactosamine (GalN) hepatitis we found that large amounts of liver membrane-bound C-CAM are secreted or shed into blood. The serum level of another liver membrane-bound protein, LI-cadherin, is not increased. It was shown that C-CAM is also present in bile fluid, and for the first time that C-CAM is present in the urine of rats with liver diseases. A particularly high concentration was measured in the urine of rats suffering from GalN hepatitis.
Lucka
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Institut für Molekularbiologie und Biochemie, Universitätklinikum Benjamin Franklin, Freie Universität Berlin, Berlin-Dahlem, Germany. lucka@zedat.fu-berlin.de
Sel
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Danker
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Horstkorte
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Reutter
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D000818:Animals; D015703:Antigens, CD; D001646:Bile / Q000737:chemistry; D001652:Bile Ducts / Q000502:physiology; D015153:Blotting, Western; D015820:Cadherins; D002272:Carcinoembryonic Antigen / Q000032:analysis / Q000097:blood / Q000652:urine; D002352:Carrier Proteins / Q000097:blood; D002448:Cell Adhesion; D015815:Cell Adhesion Molecules; D004195:Disease Models, Animal; D005688:Galactosamine; D005690:Galactose / Q000031:analogs & derivatives; D006023:Glycoproteins / Q000032:analysis* / Q000097:blood / Q000652:urine; D006520:Hepatitis, Animal / Q000097:blood / Q000139:chemically induced / Q000378:metabolism* / Q000652:urine; D008026:Ligation; D008114:Liver Neoplasms, Experimental / Q000097:blood / Q000378:metabolism* / Q000652:urine; D008562:Membrane Glycoproteins / Q000032:analysis / Q000097:blood / Q000652:urine; D026901:Membrane Transport Proteins; D051381:Rats; D011915:Rats, Inbred BUF; D017208:Rats, Wistar
D015703:Antigens, CD; C069847:CD66 antigens; C490584:CDH17 protein, human; D015820:Cadherins; D002272:Carcinoembryonic Antigen; D002352:Carrier Proteins; C490586:Cdh17 protein, rat; D015815:Cell Adhesion Molecules; D006023:Glycoproteins; D008562:Membrane Glycoproteins; D026901:Membrane Transport Proteins; C067802:intestinal peptide-proton cotransporter; C019327:2-deoxy-lyxo-hexose; D005688:Galactosamine; D005690:Galactose