title: Changes in the content of rat epididymal fluid induced by prolonged treatment with tamoxifen.
abstract: Prolonged treatment with tamoxifen induces changes in the male reproductive tract in rats. In this study changes in the protein content of the rat epididymal fluid as a consequence of prolonged treatment with tamoxifen are reported. Among five lysosomal enzymes measured in the epididymal fluid, alpha-mannosidase (alpha-MAN) significantly diminished, but other enzymes did not. Electrophoretic analysis of fluids showed that proteins of estimated molecular weight 25, 60, 80-85 and 180 kDa decreased in the treated rats. We also detected an increase in the binding of beta-galactosidase (beta-GAL) to caudal spermatozoa in treated rats. These changes may be related in part to the loss of fertilizing capacity of spermatozoa after tamoxifen treatment.
authors.affiliation: Instituto de Histología y Embriología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Belmonte
authors.forename: M
authors.initials: M
authors.lastname: Maturano
authors.forename: M F
authors.initials: MF
authors.lastname: Bertini
authors.forename: E
authors.initials: E
authors.lastname: Pusiol
authors.forename: T
authors.initials: T
authors.lastname: Sartor
authors.forename: M A
authors.initials: MA
authors.lastname: Sosa
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000201:enzymology*; D004822:Epididymis / Q000033:anatomy & histology / Q000201:enzymology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology*; D008297:Male; D008361:Mannosidases / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011467:Prostate / Q000033:anatomy & histology; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013094:Spermatozoa / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology*; D013739:Testosterone / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D004965:Estrogen Antagonists; D011506:Proteins; D013629:Tamoxifen; D013739:Testosterone; D008361:Mannosidases; D001616:beta-Galactosidase; D043323:alpha-Mannosidase