Determination of celiprolol and oxprenolol in human plasma by high-performance liquid chromatography and the analytical error function.
Two reversed-phase HPLC methods with UV detection to quantify celiprolol and oxprenolol in human plasma are described. The analytical methods for the determination of both drugs used the same reversed-phase HPLC column, mobile phase and extraction procedure. Linearity was obtained in the ranges 15.63-1000 and 25-800 ng/ml for celiprolol and oxprenolol, respectively. Intra-day and inter-day variation was lower than 14%. After validation of the methods, analytical error functions were established as S.D. (ng/ml)=3.096+0.041C for celiprolol and S.D. (ng/ml)=8.906+8.075x10(-8)C3 for oxprenolol.
Braza
A J
AJ
Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Barcelona, Spain.
Modamio
P
P
Mariño
E L
EL
D000319:Adrenergic beta-Antagonists / Q000097:blood*; D017272:Celiprolol / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010096:Oxprenolol / Q000097:blood*; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000319:Adrenergic beta-Antagonists; D010096:Oxprenolol; D017272:Celiprolol