Beta2-adrenergic agonist residues: simultaneous methyl- and butylboronic derivatization for confirmatory analysis by gas chromatography-mass spectrometry.
A derivatization procedure for confirmatory residue analysis of beta2-agonists is described. Methyl (MBA) and butyl (BBA) boronic acids are simultaneously used for the derivatization of tulobuterol, mabuterol, mapenterol, salbutamol, clenproperol, clenbuterol, clenpenterol and bromobuterol by GC-MS determination. A temperature of 55 degrees C during 60 min was selected as optimal temperature-time condition for simultaneous MBA and BBA beta2-agonists derivatization. It was also observed that stability of boronic derivatives was maintained at -20 degrees C over a period of four days. The proposed methodology was tested in urine and it could be applied for confirmatory residue analysis of clenbuterol-like compounds.
Ramos
F
F
Laboratório de Bromatologia, Nutrição e Hidrologia, Faculdade de Farmácia, Universidade de Coimbra, Portugal.
Santos
C
C
Silva
A
A
da Silveira
M I
MI
D000318:Adrenergic beta-Agonists / Q000737:chemistry* / Q000652:urine*; D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002417:Cattle; D002976:Clenbuterol / Q000031:analogs & derivatives / Q000737:chemistry / Q000652:urine; D004355:Drug Stability; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D000432:Methanol; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013696:Temperature; D013997:Time Factors
D000318:Adrenergic beta-Agonists; D001896:Boron Compounds; C030433:n-butylboronic acid; C116999:methaneboronic acid; C044573:mabuterol; D002976:Clenbuterol; D000432:Methanol