Real-time imaging of gene expression in single living cells.
Recent advances in reporter gene technologies are now allowing us to image gene transcription at the single cell level, using either fluorescence or luminescence microscopy. Here, the basis of these techniques is outlined and their advantages and disadvantages in various biological systems are discussed.
Rutter
G A
GA
Department of Biochemistry School of Medical Sciences University of Bristol Bristol BS8 1TD UK.
Kennedy
H J
HJ
Wood
C D
CD
White
M R
MR
Tavaré
J M
JM
D000818:Animals; D002477:Cells / Q000378:metabolism / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*
D005456:Fluorescent Dyes