Antitumor action of bovine seminal ribonuclease.
Unlike the bovine pancreatic ribonuclease (RNAase A), bovine seminal ribonuclease (BS RNAase) displays various biological activities including antitumor cytotoxicity. To learn more about its antitumor activity, we investigated BS RNAase effect on athymic nude mice bearing various tumors. BS RNAase (250 micrograms per mouse per day) was administered to the mice with prostate carcinoma for three weeks by three different routes (intraperitoneally--i.p., subcutaneously--s.c., and intratumorally-i.t.). Administration i.p. was ineffective, while s.c. administration reduced significantly size of tumors and i.t. administration abolished half of the tumors in treated mice. The i.t. administration of BS RNase to nude mice bearing melanoma showed even better results. Eighty % of mice were without tumors and in the other mice the tumors were significantly diminished. The best antitumor effect was obtained in case of seminoma. All mice bearing this tumor were cured after ten doses of BS RNAase.
Poucková
P
P
Institute of Biophysics, 1st School of Medicine, Charles University, Prague, Czech Republic.
Soucek
J
J
Matousek
J
J
Zadinová
M
M
Hlousková
D
D
Polívková
J
J
Navrátil
L
L
D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D002417:Cattle; D004354:Drug Screening Assays, Antitumor; D004722:Endoribonucleases / Q000008:administration & dosage / Q000627:therapeutic use*; D005260:Female; D008297:Male; D008545:Melanoma / Q000188:drug therapy; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy*; D011471:Prostatic Neoplasms / Q000188:drug therapy; D018239:Seminoma / Q000188:drug therapy; D014183:Transplantation, Heterologous
D000970:Antineoplastic Agents; D004722:Endoribonucleases; C032523:ribonuclease SPL