[Conservative regions of the gene of thermostable enterotoxin in Enterobacteriaceae--the principal possibility of their practical use].
The nucleotide sequences connected with the production of thermostable enterotoxins (ST) by the representatives of Enterobacteriaceae were analyzed. The conservative area sized up to 30 pairs of nucleotides at the 3'-end of all ST-genes, present in the bank, and responsible for the enterotoxicity of ST-toxin molecules was detected. On its basis 3 oligonucleotides were synthesized; 2 of them were used as primers in experiments on the amplification of the DNA of enterotoxigenic strains of Citrobacter spp., Escherichia coli and Yersinia spp. and the third one was used as a probe in experiments on dot-blot hybridization with the DNA of the above-mentioned cultures. The universal diagnostic test system making it possible to detect the ST-enterotoxin of opportunistic enterobacteria irrespective of their species was proposed.
Mavziutov
A R
AR
Bashkir State Medical University, Ufa, Russia.
Akhunov
E D
ED
Bondarenko
V M
VM
Gabidullin
Z G
ZG
Tuĭgunov
M M
MM
D000595:Amino Acid Sequence; D004355:Drug Stability; D004755:Enterobacteriaceae / Q000235:genetics*; D004768:Enterotoxins / Q000235:genetics*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013696:Temperature
D004768:Enterotoxins
Konservativnye uchastki gena termostabil'nogo énterotoksina Enterobacteriaceae--printsipial'naia vozmozhnost' ikh prakticheskogo ispol'zovaniia.