PsbY, a novel manganese-binding, low-molecular-mass protein associated with photosystem II.
We describe two related manganese-binding polypeptides with L-arginine metabolizing enzyme activity that can be detected as distinct components (designated PsbY-A1 and PsbY-A2, previously called L-AME) in membranes containing Photosystem II (PS II) from spinach. The polypeptides are bitopic and appear to exist in a heterodimeric form, but only in the chlorophyll a/b lineage of plants. Both proteins are encoded in the nucleus. In spinach and in Arabidopsis thaliana they are both derived from a single-copy gene (psbY) that is translated into a precursor polyprotein of approximately 20 kDa. The processing of the polyprotein is complex and includes at least four cleavage steps. Both polypeptides are exposed N-terminally to the lumenal and C-terminally to the stromal face of the thylakoid membrane.
Gau
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AE
Biologie VIII: Zellphysiologie, Universität Bielefeld, Germany.
Thole
H H
HH
Sokolenko
A
A
Altschmied
L
L
Hermann
R G
RG
Pistorius
E K
EK
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D029681:Arabidopsis Proteins; D001120:Arginine / Q000378:metabolism; D001483:Base Sequence; D001692:Biological Transport; D019607:Brassicaceae / Q000235:genetics*; D002451:Cell Compartmentation; D002467:Cell Nucleus / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D019143:Evolution, Molecular; D018628:Gene Dosage; D015723:Gene Library; D008345:Manganese / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010940:Plant Proteins; D011498:Protein Precursors / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D018724:Spinacia oleracea / Q000235:genetics; D014512:Ureohydrolases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; D011498:Protein Precursors; D008345:Manganese; D001120:Arginine; C492035:PSBY protein, Arabidopsis; C115697:PsbY protein, Spinacia oleracea; D014512:Ureohydrolases