title: Brugia malayi in Mastomys coucha: establishment in immunosuppressed animals.
abstract: Investigations on various aspects of human filariasis using target filarial parasite, Brugia malayi is jeopardised to a great extent due to its prolonged incubation period and poor harvest from the existing experimental animal models. To obviate these difficulties it was decided to establish B. malayi infection in immunosuppressed Mastomys coucha. Cortisone, a well-known immunosuppressant, was used at 10-mg/kg dose level subcutaneously in two courses each of 5 days duration. The first course was administered 1 week before and the second, 1 week after infective exposure. Mastomys were exposed either with 100 or 200 L3 each. Untreated age-matched animals were also exposed simultaneously. The minimum prepatent period was observed to be 90.7 days in immunosuppressed animals exposed to 200 L3. The course of microfilaraemia in immunosuppressed and control animals was identical up to 180 days of observation period. However, the adult worm recovery from the former group of mastomys was higher. It is surmised that exposure with B. malayi L3 in immunosuppressed mastomys would be of great advantage in getting larger harvests of adult worms of B. malayi.
authors.affiliation: Division of Parasitology, Central Drug Research Institute, Lucknow, India.
authors.forename: K
authors.initials: K
authors.lastname: Tyagi
authors.forename: P K
authors.initials: PK
authors.lastname: Murthy
authors.forename: R K
authors.initials: RK
authors.lastname: Chatterjee
mesh_terms: D000818:Animals; D017178:Brugia malayi / Q000254:growth & development*; D003348:Cortisone / Q000008:administration & dosage; D004195:Disease Models, Animal; D005368:Filariasis / Q000469:parasitology*; D007165:Immunosuppression Therapy; D007814:Larva / Q000502:physiology; D008297:Male; D009115:Muridae / Q000469:parasitology*; D018512:Parasitemia / Q000469:parasitology
chemical_list: D003348:Cortisone