Biodiversity of an Acinetobacter population isolated from activated sludge.
A culturable microbial community from a sewage treatment plant collecting mainly surfactant-enriched wastes was selected on minimal medium containing two nonylphenol ethoxylates as sole carbon source. Biodiversity of the community was assessed on fifty randomly chosen isolates by a combination of molecular techniques. Isolates were first analysed by amplified 16S ribosomal DNA restriction analysis (ARDRA); most of them (75%) were assigned to the genus Acinetobacter on the basis of 16S ribosomal DNA sequencing. Random amplified polymorphic DNA (RAPD) fingerprinting and the analysis of plasmid content showed a high degree of genetic variability and suggested a marked horizontal gene transfer.
Barberio
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Dipartimento di Biologia Animale e Genetica, via Romana 17-19, I-50125 Firenze, Italy.
Fani
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D000150:Acinetobacter / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001483:Base Sequence; D004275:DNA, Ribosomal / Q000737:chemistry; D017753:Ecosystem; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012722:Sewage