How the ovarian follicle of Podarcis sicula recycles the DNA of its nurse, regressing follicle cells.
In Podarcis sicula specialized follicle cells send reserve materials to the previtellogenic oocyte via intercellular bridges. Immediately before the onset of vitellogenesis this transferring becomes particularly massive so that the cell volume significantly reduces, meanwhile in the nucleus the morphological alterations typical of apoptosis appear. To clarify why these follicle cells are not simply fully resorbed by the oocyte and to determine whether their DNA is discarded or recycled, we carried out a series of morphological and biochemical investigations. The finding that large macromolecular scaffolds are formed and that these are able to retain the DNA until it is extensively cut by two different endonucleases suggests that regression of the follicle cells is programmed and that the fate of their DNA is strictly controlled. Following its genetical neutralization via fragmentation, the DNA is apparently recycled by being transferred into the oocyte via the intercellular bridges, that, in fact, remain open until the very late stages of cell regression. The small DNA fragments reaching the oocyte cytoplasm would not interfere with meiosis completion but could significantly contribute to the stock of reserve materials to the advantage of the growing oocyte and/or developing embryo.
De Caro
M
M
Department of Evolutive and Comparative Biology, University of Naples Federico II, Italy.
Indolfi
P
P
Iodice
C
C
Spagnuolo
S
S
Tammaro
S
S
Motta
C M
CM
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008116:Lizards / Q000378:metabolism*; D008854:Microscopy, Electron; D006080:Ovarian Follicle / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism; D014818:Vitellogenesis
D004247:DNA; D011503:Transglutaminases