title: Isolation of translactone-containing triterpenes with thrombin inhibitory activities from the leaves of Lantana camara.
abstract: Methanolic extracts prepared from the leaves of Lantana camara have been found to inhibit human thrombin. An assay, in which thrombin activity is measured as a function of clot formation from fibrinogen, was used to guide the fractionation and purification of five principal active constituents (1-5), which were all characterized as 5,5-trans-fused cyclic lactone-containing euphane triterpenes.
authors.affiliation: GlaxoWellcome Research and Development, Medicines Research Centre, Gunnels Wood Road, Stevenage, SG1 2NY, UK. mjon7483@glaxowellcome.co.uk
authors.forename: M J
authors.initials: MJ
authors.lastname: O'Neill
authors.forename: J A
authors.initials: JA
authors.lastname: Lewis
authors.forename: H M
authors.initials: HM
authors.lastname: Noble
authors.forename: S
authors.initials: S
authors.lastname: Holland
authors.forename: C
authors.initials: C
authors.lastname: Mansat
authors.forename: J E
authors.initials: JE
authors.lastname: Farthing
authors.forename: G
authors.initials: G
authors.lastname: Foster
authors.forename: D
authors.initials: D
authors.lastname: Noble
authors.forename: S J
authors.initials: SJ
authors.lastname: Lane
authors.forename: P J
authors.initials: PJ
authors.lastname: Sidebottom
authors.forename: S M
authors.initials: SM
authors.lastname: Lynn
authors.forename: M V
authors.initials: MV
authors.lastname: Hayes
authors.forename: C J
authors.initials: CJ
authors.lastname: Dix
mesh_terms: D002240:Carbohydrate Sequence; D018360:Crystallography, X-Ray; D005340:Fibrinogen / Q000037:antagonists & inhibitors; D006801:Humans; D008969:Molecular Sequence Data; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013917:Thrombin / Q000037:antagonists & inhibitors*; D014315:Triterpenes / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; D005340:Fibrinogen; D013917:Thrombin