Accelerated viraemia in cats vaccinated with fixed autologous FIV-infected cells.
We have vaccinated cats with fixed autologous FIV infected PBMC to determine whether autologous presentation of antigen is capable of inducing a protective immune response against homologous challenge. To this end autologous PBMC were infected with a FIV molecular clone (19k1). When infection was established, cells were inactivated by dialysis against paraformaldehyde. Upon vaccination, cats developed a virus specific immune response as measured by ELISA against the Gag protein of FIV. No antibodies against the envelope protein were detected with a peptide ELISA. Virus neutralizing antibodies however could be detected with a neutralization assay based on infection of CrFK cells, but not in an assay based on infection of primary T-cells. Although vaccination led to the induction of these virus-specific immune responses, vaccinated cats were not protected against homologous challenge but showed an accelerated viraemia upon infection. This was shown both by PCR and cell-associated viral load. The possible mechanisms underlying this observation are discussed in this paper.
Karlas
J A
JA
Institute of Virology, Erasmus University Rotterdam, The Netherlands.
Siebelink
K H
KH
v Peer
M A
MA
Huisman
W
W
Rimmelzwaan
G F
GF
Osterhaus
A D
AD
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D016913:Blood Component Transfusion; D001804:Blood Transfusion, Autologous; D002415:Cats; D017931:DNA Primers / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis; D016181:Feline Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000517:prevention & control*; D016089:Immunodeficiency Virus, Feline / Q000235:genetics / Q000276:immunology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology*; D014611:Vaccination / Q000662:veterinary*; D019562:Viral Load; D014765:Viral Vaccines / Q000008:administration & dosage*; D014766:Viremia / Q000209:etiology* / Q000276:immunology
D000914:Antibodies, Viral; D017931:DNA Primers; D004279:DNA, Viral; D014765:Viral Vaccines