Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats.
Limited experimental access to the central nervous system (CNS) is a key problem in the study of human neural development, disease, and regeneration. We have addressed this problem by generating neural chimeras composed of human and rodent cells. Fetal human brain cells implanted into the cerebral ventricles of embryonic rats incorporate individually into all major compartments of the brain, generating widespread CNS chimerism. The human cells differentiate into neurons, astrocytes, and oligodendrocytes, which populate the host fore-, mid-, and hindbrain. These chimeras provide a unique model to study human neural cell migration and differentiation in a functional nervous system.
Brüstle
O
O
Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4092, USA. brustle@uni-bonn.de
Choudhary
K
K
Karram
K
K
Hüttner
A
A
Murray
K
K
Dubois-Dalcq
M
M
McKay
R D
RD
D000818:Animals; D001709:Biotechnology; D001921:Brain / Q000166:cytology / Q000196:embryology*; D016380:Brain Tissue Transplantation; D002454:Cell Differentiation; D002552:Cerebral Ventricles / Q000166:cytology / Q000196:embryology; D002678:Chimera; D005260:Female; D016332:Fetal Tissue Transplantation; D006801:Humans; D008959:Models, Neurological; D009474:Neurons / Q000166:cytology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology