Identification of various exon-deleted progesterone receptor mRNAs in human endometrium and ovarian endometriosis.
We demonstrated the expression of various exon-deleted progesterone receptor (PR) variant mRNAs in human uterine endometrium and ovarian endometriosis using the reverse transcription-polymerase chain reaction-DNA sequencing analyses. In addition to PR wild-type mRNA, various exon-deleted PR variant mRNAs were identified in all samples analyzed. The sequence of these variants showed a perfect junction between exons surrouding the deletion area. PR wild-type, exon 6-deleted, exon 4-deleted, exon 5, 6-deleted and exon 4, 5, 6-deleted PR variant mRNAs were observed in all samples analyzed. Exon 4, 6-deleted PR mRNA was observed only in ovarian endometriosis. This is the first study to demonstrate the coexpression of various PR exon-deleted variant mRNAs with the wild-type in uterine endometrium and ovarian endometriosis. All resulting variant proteins might indicate functional diversity and modify the progestational action of wild-type PR, and thus be involved in the pathophysiology of ovarian endometriosis.
Misao
R
R
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu City, Japan.
Sun
W S
WS
Iwagaki
S
S
Fujimoto
J
J
Tamaya
T
T
D000328:Adult; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004715:Endometriosis / Q000235:genetics* / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005091:Exons; D005260:Female; D014644:Genetic Variation; D006801:Humans; D010049:Ovarian Diseases / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion
D017931:DNA Primers; D012333:RNA, Messenger; D011980:Receptors, Progesterone