Optimization of an antibiotic residue screening test, based on inhibition of Bacillus subtilis BGA, with experimental design.
Experimental design was applied to an agar-diffusion inhibition test for the screening of residues of veterinary antimicrobial drugs in slaughter animals. The effects and interactions of four independent parameters were studied: the dextrose content of the culture medium, the trimethoprim concentration in the culture medium, the thickness of the agar-layer and the preincubation time. The effects on the inhibition zones of sulphadimidine, oxytetracycline, streptomycin and tylosin, substances of four different antibiotic groups were measured. An optimized multi-residue test was established with the parameter values that yielded the best sensitivity for the four substances.
Koenen-Dierick
K
K
Ministry of Public Health, Scientific Institute of Public Health-Louis Pasteur, Department of Pharmacobromatology, Brussels, Belgium.
De Beer
J O
JO
D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001412:Bacillus subtilis; D001681:Biological Assay; D015169:Colony Count, Microbial; D004350:Drug Residues; D005506:Food Contamination / Q000032:analysis*; D008460:Meat / Q000032:analysis*; D010118:Oxytetracycline / Q000032:analysis; D013307:Streptomycin / Q000032:analysis; D013418:Sulfamethazine / Q000032:analysis; D015645:Tylosin / Q000032:analysis; D019155:Veterinary Drugs / Q000032:analysis*
D000900:Anti-Bacterial Agents; D019155:Veterinary Drugs; D013418:Sulfamethazine; D010118:Oxytetracycline; D013307:Streptomycin; D015645:Tylosin