Transient alteration in intestinal permeability to technetium Tc99m diethylenetriaminopentaacetate during the prodromal stages of alimentary laminitis in ponies.
OBJECTIVE
To determine whether mucosal permeability is altered during the prodromal stages of alimentary laminitis.


METHODS
15 healthy adult ponies.


METHODS
intestinal permeability was evaluated for control ponies (n = 5) and for ponies 4 to 12 (n = 5) and 20 to 28 (n = 5) hours after administration of carbohydrate overload. Mucosal permeability was determined by measuring the percentage of orally administered technetium Tc99m diethylenetriaminopentaacetate (99mTc-DTPA) excreted in urine during an 8-hour period, then measuring blood radioactivity at hourly intervals. Plasma endotoxin-like activity was measured by use of a chromogenic Limulus amebocyte assay.


RESULTS
Urinary excretion of 99mTc-DTPA was 2.45% of administered dose for control ponies, and was 16.67% of administered dose 4 to 12 hours and 3.57% of administered dose 20 to 28 hours after administration of carbohydrate.


CONCLUSIONS
A marked but transient increase in intestinal permeability was observed early in the prodromal stages of alimentary laminitis.


CONCLUSIONS
Absorption of substances from the intestine may be an initiating event in alimentary laminitis.
Weiss
D J
DJ
Department of Veterinary PathoBiology, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.
Evanson
O A
OA
MacLeay
J
J
Brown
D R
DR
D000818:Animals; D002241:Carbohydrates / Q000008:administration & dosage; D005534:Foot Diseases / Q000150:complications / Q000503:physiopathology / Q000662:veterinary*; D005767:Gastrointestinal Diseases / Q000150:complications / Q000503:physiopathology / Q000662:veterinary*; D006734:Horse Diseases / Q000503:physiopathology*; D006736:Horses; D007408:Intestinal Absorption; D007794:Lameness, Animal / Q000150:complications / Q000503:physiopathology*; D010539:Permeability; D016284:Technetium Tc 99m Pentetate / Q000493:pharmacokinetics* / Q000652:urine
D002241:Carbohydrates; D016284:Technetium Tc 99m Pentetate