title: Distribution of V-ATPase in rat salivary glands.
abstract: Using immunohistochemistry we have investigated the presence and cellular distribution of the 31-kDa subunit of vacuolar-type H+-ATPase (V-ATPase) in secretory endpieces and the duct system of rat major salivary glands. In all three salivary glands studied the 31-kDa subunit of V-ATPase was not expressed in secretory endpieces. In rat parotid gland V-ATPase was luminally located in main excretory and striated duct cells. In contrast, both rat submandibular and sublingual glands showed a diffuse intracellular V-ATPase distribution. The differences in V-ATPase immunolocalization in rat salivary glands probably reflect the structural heterogeneity of the different glands. The data also suggest that the duct systems of major salivary glands may modify the H+ and HCO3- concentration of the final saliva in different ways.
authors.affiliation: Dept. Anatomy, University of the Saarland, Homburg/Saar, Germany. anerou@krzsun.med-rz.uni-sb.de
authors.forename: E
authors.initials: E
authors.lastname: Roussa
authors.forename: F
authors.initials: F
authors.lastname: Thévenod
mesh_terms: D000818:Animals; D008297:Male; D010306:Parotid Gland / Q000201:enzymology; D017494:Proton Pumps / Q000032:analysis; D006180:Proton-Translocating ATPases / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D018987:Salivary Ducts / Q000201:enzymology; D012469:Salivary Glands / Q000201:enzymology*; D013361:Sublingual Gland / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology; D025262:Vacuolar Proton-Translocating ATPases; D014617:Vacuoles / Q000201:enzymology*
chemical_list: D017494:Proton Pumps; D025262:Vacuolar Proton-Translocating ATPases; D006180:Proton-Translocating ATPases