Wounding and chemicals induce expression of the Arabidopsis thaliana gene Thi2.1, encoding a fungal defense thionin, via the octadecanoid pathway.
In seedlings of Arabidopsis thaliana the thionin gene Thi2.1 is inducible by methyl jasmonate, wounding, silver nitrate, coronatine, and sorbitol. We have used a biochemical and genetic approach to test the signal transduction of these different inducers. Both exogenously applied jasmonates and jasmonates produced endogenously upon stress induction, lead to GUS expression in a Thi2.1 promoter-uidA transgenic line. No GUS expression was observed in a coil mutant background which lacks jasmonate perception whereas methyl jasmonate and coronatine but not the other inducers were able to overcome the block in jasmonic acid production in a fad3-2 fad7-2 fad8 mutant background. Our results show conclusively that all these inducers regulate Thi2-1 gene expression via the octadecanoid pathway.
Bohlmann
H
H
Swiss Federal Institute of Technology (ETH), Institute of Plant Sciences, ETH-Zentrum, Zürich, Switzerland. holger.bohlmann@ipw.biol.ethz.ch
Vignutelli
A
A
Hilpert
B
B
Miersch
O
O
Wasternack
C
C
Apel
K
K
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins; D003517:Cyclopentanes / Q000037:antagonists & inhibitors / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D009154:Mutation; D054883:Oxylipins; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D012835:Silver Nitrate / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D013229:Stearic Acids / Q000378:metabolism*
D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D013229:Stearic Acids; C097482:THI2.1 protein, Arabidopsis; D013012:Sorbitol; C011006:jasmonic acid; D012835:Silver Nitrate