[Establishing an umbilical cord blood bank for unrelated allogenic stem cell transplantation].
We report the establishment of a cord-blood bank in a routine hematological laboratory. Cord-blood collection was performed with placenta in utero by a trained team and immediately sent to the cord-blood bank. There, 6.8 ml cord-blood was used for analysis of nucleated cell counts, counts of CD34-positive cells, CFU's, complete HLA-typing, ABO and Rhesus blood groups, bacteriologic cultures and serology for HIV 1 and 2, HbsAg, HVC, CMV, syphilis and toxoplasmosis. The cord-blood collection was frozen and conserved at -192 degrees C. From each cord-blood vials of DNA, viable cells and plasma were cryopreserved. Between June 1997 and April 1998, 54 cord-bloods were collected. 40 of them were cryo-preserved, and 14 discarded because of low cell counts. The median volume was 109 ml with 1.4 x 10(9) nucleated cells. The in vitro capacity of proliferation of the cord-blood correlated well with the absolute counts of CD34-positive cells (r = 0.93), moderately with the relative counts of CD34 (r = 0.68) as well as the nucleated cells (r = 0.70), poorly with the volume (r = 0.44). Three of the 40 (7.5%) cord-blood products contained a bacterial contamination. This study shows that a cord-blood bank can be organised in a routine hematological laboratory, which is familiar with transplantation products. However, the procedure is time consuming, expensive and requires a highly qualified team and specialised technical equipment.
Tichelli
A
A
Departement Zentrallaboratorium, Kantonsspital Basel.
Surbek
D
D
Huxol
H
H
Schmolck
C
C
John
L
L
Wicki
R
R
Hoffmann
T
T
Wodnar-Filipowicz
A
A
Passweg
J
J
Kühne
T
T
Imbach
P
P
Holzgreve
W
W
Gratwohl
A
A
D001771:Blood Banks / Q000458:organization & administration*; D005312:Fetal Blood / Q000166:cytology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007231:Infant, Newborn; D010348:Patient Care Team / Q000458:organization & administration; D013557:Switzerland
Aufbau einer Nabelschnurblutbank für unverwandte allogene Stammzelltransplantationen.