title: Interaction of alpha-phenyl-N-tert-butyl nitrone and alternative electron acceptors with complex I indicates a substrate reduction site upstream from the rotenone binding site.
abstract: Mitochondrial complexes I, II, and III were studied in isolated brain mitochondrial preparations with the goal of determining their relative abilities to reduce O2 to hydrogen peroxide (H2O2) or to reduce the alternative electron acceptors nitroblue tetrazolium (NBT) and diphenyliodonium (DPI). Complex I and II stimulation caused H2O2 formation and reduced NBT and DPI as indicated by dichlorodihydrofluorescein oxidation, nitroformazan precipitation, and DPI-mediated enzyme inactivation. The O2 consumption rate was more rapid under complex II (succinate) stimulation than under complex I (NADH) stimulation. In contrast, H2O2 generation and NBT and DPI reduction kinetics were favored by NADH addition but were virtually unobservable during succinate-linked respiration. NADH oxidation was strongly suppressed by rotenone, but NADH-coupled H2O2 flux was accelerated by rotenone. Alpha-phenyl-N-tert-butyl nitrone (PBN), a compound documented to inhibit oxidative stress in models of stroke, sepsis, and parkinsonism, partially inhibited complex I-stimulated H2O2 flux and NBT reduction and also protected complex I from DPI-mediated inactivation while trapping the phenyl radical product of DPI reduction. The results suggest that complex I may be the principal source of brain mitochondrial H2O2 synthesis, possessing an "electron leak" site upstream from the rotenone binding site (i.e., on the NADH side of the enzyme). The inhibition of H2O2 production by PBN suggests a novel explanation for the broad-spectrum antioxidant and antiinflammatory activity of this nitrone spin trap.
authors.affiliation: Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
authors.forename: K
authors.initials: K
authors.lastname: Hensley
authors.forename: Q N
authors.initials: QN
authors.lastname: Pye
authors.forename: M L
authors.initials: ML
authors.lastname: Maidt
authors.forename: C A
authors.initials: CA
authors.lastname: Stewart
authors.forename: K A
authors.initials: KA
authors.lastname: Robinson
authors.forename: F
authors.initials: F
authors.lastname: Jaffrey
authors.forename: R A
authors.initials: RA
authors.lastname: Floyd
mesh_terms: D000818:Animals; D001665:Binding Sites / Q000502:physiology; D001713:Biphenyl Compounds / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D003497:Cyclic N-Oxides; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000187:drug effects*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D009589:Nitrogen Oxides / Q000494:pharmacology*; D009861:Onium Compounds / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012402:Rotenone / Q000378:metabolism* / Q000494:pharmacology; D019802:Succinic Acid / Q000494:pharmacology
chemical_list: D001713:Biphenyl Compounds; D003497:Cyclic N-Oxides; D009589:Nitrogen Oxides; D009861:Onium Compounds; D012402:Rotenone; D009243:NAD; C031291:diphenyliodonium; D009580:Nitroblue Tetrazolium; C029217:phenyl-N-tert-butylnitrone; D019802:Succinic Acid; D006861:Hydrogen Peroxide; D016660:NAD(P)H Dehydrogenase (Quinone)