title: 8-Isoprostane as a biomarker of oxidative stress in interstitial lung diseases.
abstract: Oxidative stress contributes to the pathophysiology of interstitial lung diseases, such as cryptogenic fibrosing alveolitis (CFA), fibrosing alveolitis associated with systemic sclerosis (FASSc) and sarcoidosis. F2-isoprostanes are a series of prostaglandin (PG) F2-like compounds produced in vivo independent of cyclooxygenase, as products of the radical-catalyzed lipid peroxidation. Measurement of the concentrations of F2-isoprostanes has proved to be valuable in assessing oxidative stress in vivo. The aim of this study was to measure 8-epi-PGF2alpha concentrations, one of the most abundant F2-isoprostane in humans, in bronchoalveolar lavage (BAL) in normal subjects and to compare them to those observed in patients with CFA (n = 9), FASSc (n = 8) and sarcoidosis (n = 10). 8-epi-PGF2alpha was detectable in BAL fluid in normal subjects (9.6 +/- 0.8 pg/ml) and its concentrations were increased approximately 5-fold in patients with CFA (47.4 +/- 7.0, p < 0.001) and FASSc (43.2 +/- 3.3, p < 0. 001). 8-epi-PGF2alpha was also increased in patients with sarcoidosis, although to a lesser extent (12.0 +/- 0.70 pg/ml, p < 0. 01). No correlation between 8-epi-PGF2alpha and either lung function tests or BAL cell types was observed in any group of patients. Our study shows that the level of oxidative stress is enhanced in patients with interstitial lung diseases as reflected by increased concentrations of 8-epi-PGF2alpha in BAL fluid.
authors.affiliation: Imperial College School of Medicine at the National Heart and Lung Institute, Department of Thoracic Medicine, Interstitial Lung Disease Unit, Royal Brompton Hospital, London, United Kingdom.
authors.forename: P
authors.initials: P
authors.lastname: Montuschi
authors.forename: G
authors.initials: G
authors.lastname: Ciabattoni
authors.forename: P
authors.initials: P
authors.lastname: Paredi
authors.forename: P
authors.initials: P
authors.lastname: Pantelidis
authors.forename: R M
authors.initials: RM
authors.lastname: du Bois
authors.forename: S A
authors.initials: SA
authors.lastname: Kharitonov
authors.forename: P J
authors.initials: PJ
authors.lastname: Barnes
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D001999:Bronchoscopy; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D028441:F2-Isoprostanes; D005260:Female; D005609:Free Radicals / Q000032:analysis; D006801:Humans; D016014:Linear Models; D015227:Lipid Peroxidation; D017563:Lung Diseases, Interstitial / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis; D018384:Oxidative Stress; D011658:Pulmonary Fibrosis / Q000378:metabolism; D012507:Sarcoidosis / Q000378:metabolism; D012595:Scleroderma, Systemic / Q000378:metabolism; D014057:Tomography, X-Ray Computed
chemical_list: D015415:Biomarkers; D028441:F2-Isoprostanes; D005609:Free Radicals; C075750:8-epi-prostaglandin F2alpha; D009569:Nitric Oxide; D015237:Dinoprost