Cytokinins as inhibitors of plant amine oxidase.
The interaction of pea seedling amine oxidase with cytokinins was examined to probe a possible connection between cytokinin oxidase and amine oxidase by determining whether cytokinins are substrates or inhibitors of the latter. Kinetic measurements suggest that cytokinins are weak competitive inhibitors of amine oxidase while their behaviour as substrates was not observed. The absence of enzymatic activity with cytokinins as substrates denies the identity or even any similarity of these two enzymes which was previously considered [Hare, P.D. and van Staden, J. (1994) J. Physiol. Plant., 91, 128]. From the values of the inhibition constants obtained it seems unlikely that cytokinins take part in the regulation of amine oxidase activity in vivo. Their inhibitory effect on amine oxidase may be similar to that of some alkaloids studied earlier.
Galuszka
P
P
Department of Biochemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic. galuszka@risc.upol.cz
Sebela
M
M
Luhová
L
L
Zajoncová
L
L
Frébort
I
I
Strnad
M
M
Pec
P
P
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001667:Binding, Competitive; D003583:Cytokinins / Q000378:metabolism / Q000494:pharmacology*; D007700:Kinetics; D018532:Pisum sativum / Q000201:enzymology; D010940:Plant Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013329:Structure-Activity Relationship
D003583:Cytokinins; D010940:Plant Proteins; D006631:Amine Oxidase (Copper-Containing)