Prominent expression of presenilin-1 in senile plaques and reactive astrocytes in Alzheimer's disease brain.
Mutations in the presenilin 1 (PS-1) gene account for most cases of autosomal dominant early-onset familial Alzheimer's disease (AD). In order to elucidate the cellular expression profile of PS-1 we used a novel N-terminal monoclonal antibody against human PS-1. Immunohistochemical staining was observed strongly in senile plaques, and reactive astrocytes of gray and white matter. Neuronal immunoreactivity, however, was found to be only moderate. RT-PCR analysis of PS-1 mRNA revealed expression throughout human development as well as in human glioma cell lines. Altered PS-1 function may contribute to plaque formation in AD.
Weggen
S
S
Department of Psychiatry, University of Bonn Medical Center, Germany.
Diehlmann
A
A
Buslei
R
R
Beyreuther
K
K
Bayer
T A
TA
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics* / Q000378:metabolism*; D000911:Antibodies, Monoclonal; D001253:Astrocytes / Q000737:chemistry*; D015870:Gene Expression / Q000502:physiology; D005910:Glioma; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D008875:Middle Aged; D058225:Plaque, Amyloid / Q000737:chemistry*; D053764:Presenilin-1; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D008565:Membrane Proteins; C508853:PSEN1 protein, human; D053764:Presenilin-1; D012333:RNA, Messenger