title: Pathological and histological study on retinoblastoma.
abstract: We studied 30 cases of retinoblastoma (Rb) pathologically and histochemically. Rb cases were classified into two types, 18 cases of differentiated (D-type) and 12 undifferentiated type (U-type) according to International Classification of Diseases for Oncology(1990, WHO). D-type of Rb is found more commonly in younger infants less than 1 year, but U-type found more frequently in aged infants from 2 to 4 years. D-type Rb showed more marked necrotic areas, degeneration and calcification. Immunohistochemical stains were carried out in 21 cases of Rb by use of MIB-1, PCNA, bcl-2, Fas, BAX, NF, SP and Tunel stains, respectively. MIB-1 is a good marker of proliferation of Rb cells and its high positivity correlates closely to more extensive necrotic areas of Rb cases. There are only a few positive cells by Tunel stain suggesting apoptotic cells, but diffuse positive cells by bcl-2 and Fas and completely negative by BAX gene. From these results, it can be seen that differentiation of Rb cells may be affected by bcl-2 and Fas but not by BAX gene.
authors.affiliation: Department of Pathology, Kobe University School of Medicine, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fujisawa
authors.forename: K
authors.initials: K
authors.lastname: Itoh
authors.forename: Y
authors.initials: Y
authors.lastname: Imai
authors.forename: H
authors.initials: H
authors.lastname: Itoh
authors.forename: M
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D000367:Age Factors; D002467:Cell Nucleus / Q000473:pathology; D002675:Child, Preschool; D003937:Diagnosis, Differential; D005260:Female; D016161:Genes, Retinoblastoma; D005819:Genetic Markers; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D009336:Necrosis; D019572:Retinal Neoplasms / Q000235:genetics / Q000473:pathology*; D012175:Retinoblastoma / Q000235:genetics / Q000473:pathology*; D012737:Sex Factors
chemical_list: D005819:Genetic Markers