Cellular gene expression altered by human cytomegalovirus: global monitoring with oligonucleotide arrays.
Mechanistic insights to viral replication and pathogenesis generally have come from the analysis of viral gene products, either by studying their biochemical activities and interactions individually or by creating mutant viruses and analyzing their phenotype. Now it is possible to identify and catalog the host cell genes whose mRNA levels change in response to a pathogen. We have used DNA array technology to monitor the level of approximately 6,600 human mRNAs in uninfected as compared with human cytomegalovirus-infected cells. The level of 258 mRNAs changed by a factor of 4 or more before the onset of viral DNA replication. Several of these mRNAs encode gene products that might play key roles in virus-induced pathogenesis, identifying them as intriguing targets for further study.
Zhu
H
H
Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cong
J P
JP
Mamtora
G
G
Gingeras
T
T
Shenk
T
T
D017305:Annexin A1 / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D051546:Cyclooxygenase 2; D003587:Cytomegalovirus / Q000502:physiology*; D004247:DNA / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism / Q000821:virology; D005786:Gene Expression Regulation; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D008297:Male; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; D009838:Oligodeoxyribonucleotides; D010741:Phospholipases A / Q000235:genetics; D011401:Promoter Regions, Genetic; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000235:genetics; D012867:Skin / Q000166:cytology / Q000378:metabolism / Q000821:virology; D019699:Thrombospondins / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic
D017305:Annexin A1; D004268:DNA-Binding Proteins; D006680:HLA Antigens; D007527:Isoenzymes; C497234:MITF protein, human; D008565:Membrane Proteins; D051739:Microphthalmia-Associated Transcription Factor; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D019699:Thrombospondins; D014157:Transcription Factors; D004247:DNA; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A