title: Dioctahedral smectite neutralization activity of Clostridium difficile and Bacteroides fragilis toxins in vitro.
abstract: The neutralization activity of dioctahedral smectite for ten toxigenic Clostridium difficile and eight enterotoxigenic Bacteroides fragilis strains was studied using McCoy and HT 29/C1 cell lines, respectively. Minimalization of the cytopathic effect of C. difficile toxin B on McCoy cell lines by dioctahedral smectite dissolved in PBS was observed. After incubation with dioctahedral smectite the toxic effects of B. fragilis enterotoxins on HT/29C1 (human colon adenocarcinoma cell line) were eliminated. Best neutralization of B. fragilis toxin was achieved using dioctahedral smectite dissolved in BHI.
authors.affiliation: Department of Medical Microbiology, Institute of Biostructure Warsaw Medical Academy, Poland.
authors.forename: G
authors.initials: G
authors.lastname: Martirosian
authors.forename: G
authors.initials: G
authors.lastname: Rouyan
authors.forename: T
authors.initials: T
authors.lastname: Zalewski
authors.forename: F
authors.initials: F
authors.lastname: Meisel-Mikołajczyk
mesh_terms: D000818:Animals; D000930:Antidiarrheals / Q000494:pharmacology*; D001426:Bacterial Proteins; D001427:Bacterial Toxins / Q000037:antagonists & inhibitors*; D001441:Bacteroides fragilis / Q000187:drug effects* / Q000378:metabolism*; D002460:Cell Line; D016360:Clostridioides difficile / Q000187:drug effects* / Q000378:metabolism*; D004768:Enterotoxins / Q000037:antagonists & inhibitors; D006041:Goats; D019073:HT29 Cells; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D017640:Silicates / Q000494:pharmacology*
chemical_list: D000930:Antidiarrheals; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004768:Enterotoxins; D017640:Silicates; C016752:smecta; C041348:tcdA protein, Clostridium difficile; C031815:toxB protein, Clostridium difficile