Cytoprotection by deprenyl and tolcapone in a cell culture model of cerebral ischaemia.
Foetal rat brain aggregation cultures were exposed to a single episode of anoxia and hypoglycaemia for 30 min. Lactate dehydrogenase specific activity was estimated in the culture medium after ischaemia as a marker of lost cell integrity. Release of lactate dehydrogenase was most prominent during the first 24 hr period after the ischaemic damage, then it gradually declined. Immediately after ischaemic exposure, the cultures were treated with different concentrations of L-deprenyl or tolcapone. Significantly lower amounts of lactate dehydrogenase leaked into the culture medium during the first 24 hr after the ischaemic episode in cultures treated with deprenyl or tolcapone (1-100 nM). These results suggest that deprenyl and tolcapone may reduce cell damage after ischaemia, at doses causing enzyme inhibition.
Ekblom
J
J
Department of Neuroscience, Uppsala University, Sweden.
Tottmar
O
O
Oreland
L
L
D000818:Animals; D001577:Benzophenones / Q000494:pharmacology*; D002545:Brain Ischemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D002394:Catechol O-Methyltransferase / Q000378:metabolism; D065098:Catechol O-Methyltransferase Inhibitors; D015687:Cell Hypoxia / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection / Q000187:drug effects*; D004338:Drug Combinations; D005260:Female; D007003:Hypoglycemia / Q000378:metabolism; D020287:In Situ Nick-End Labeling; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009596:Nitrophenols; D011247:Pregnancy; D016548:Prosencephalon / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012642:Selegiline / Q000494:pharmacology*; D000077867:Tolcapone
D001577:Benzophenones; D065098:Catechol O-Methyltransferase Inhibitors; D004338:Drug Combinations; D008996:Monoamine Oxidase Inhibitors; D018696:Neuroprotective Agents; D009596:Nitrophenols; D012642:Selegiline; D000077867:Tolcapone; D007770:L-Lactate Dehydrogenase; D008995:Monoamine Oxidase; D002394:Catechol O-Methyltransferase