title: High expression of a functional cruzipain by a non-infective and non-pathogenic Trypanosoma cruzi clone.
abstract: We compared a Trypanosoma cruzi clone unable to infect or induce pathology in mice (CL-14), with virulent T. cruzi (Y and CL strains) in terms of cruzipain expression, subcellular distribution and functional activity. Our results showed that (1) intracellular Y amastigotes expressed R1 (carboxy-terminal) and R2 (catalytic) domains concentrated in cytoplasmic vesicles, while CL-14 presented R1 labelling on membrane clusters and R2 in intracellular compartments, (2) CL-14-trypomastigotes presented R1 and R2 staining preferentially on flagellar and cellular membranes, similar to CL, but different from Y strain intracellular labelling pattern, (3) flow-cytometry revealed higher expression of R1 by CL-14-trypomastigotes than virulent strains, but R2 staining similar to CL-trypomastigotes, (4) CL-14-trypomastigotes presented normal cruzipain activity in gelatin gels, but different banding patterns were found in CL-14 versus CL and Y strains. Our data rule out failure in cruzipain expression, activity or subcellular distribution as an explanation for CL-14 biological behaviour, but suggest the expression of a different isoform. These results also cast doubt on the putative role of cruzipain as a target of immunopathological responses, since high levels of functional cruzipain are expressed by a non-pathogenic T. cruzi.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Brasil.
authors.forename: C N
authors.initials: CN
authors.lastname: Paiva
authors.forename: T
authors.initials: T
authors.lastname: Souto-Padron
authors.forename: D A
authors.initials: DA
authors.lastname: Costa
authors.forename: C R
authors.initials: CR
authors.lastname: Gattass
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000032:analysis*; D003546:Cysteine Endopeptidases / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D005434:Flow Cytometry; D007150:Immunohistochemistry; D051379:Mice; D015800:Protozoan Proteins; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000201:enzymology* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D003546:Cysteine Endopeptidases; C063214:cruzipain