Enhancement of mycinamicin production by dotriacolide in Micromonospora griseorubida.
Production of macrolide antibiotic mycinamicin was greatly increased by addition of sulfate ion into the culture medium of Micromonospora griseorubida. An O-sulfate ester compound, also produced by the strain, was shown to be dotriacolide. In an M. griseorubida dotriacolide non-producing strain, the production level of mycinamicin remained low, but increased to the level of dotriacolide producing strain by the addition of dotriacolide. Dotriacolide enhanced mycinamicin production in M. griseorubida by the formation of micelles with mycinamicin. As a result, dotriacolide played a critical role in mycinamicin production in M. griseorubida.
Takenaka
S
S
Pharmaceuticals Production Technology Department, Asahi Chemical Industry Co., Ltd., Shizuoka, Japan.
Yoshida
K
K
Yamaguchi
O
O
Shimizu
K
K
Morohoshi
T
T
Kinoshita
K
K
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007783:Lactones / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018942:Macrolides; D008823:Micelles; D008847:Micromonospora / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology; D065093:beta-Lactamase Inhibitors
D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D007783:Lactones; D018942:Macrolides; D008823:Micelles; D013431:Sulfates; D065093:beta-Lactamase Inhibitors; C033798:dotriacolide