Polymorphonuclear leukocyte membrane fluidity and cytosolic Ca2+ concentration in diabetes mellitus.
We evaluated polymorphonuclear membrane (PMN) fluidity in 32 subjects with type 1 diabetes mellitus, 38 subjects with type 2 diabetes mellitus and 38 normal control subjects, by marking intact and unstimulated PMN cells with the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH). We also evaluated PMN cytosolic Ca2+ content by marking intact and unstimulated PMN cells with the fluorescent probe Fura 2-AM. PMN membrane fluidity differentiated normal subjects from type 1 and 2 diabetic subjects. The PMN cytosolic Ca2+ concentration did not discriminate type 1 and 2 diabetic subjects from normal control subjects. No statistical correlation was found between PMN membrane fluidity and PMN cytosolic Ca2+ concentration in any of the groups of subjects, nor were significant correlations found between PMN membrane fluidity and cytosolic Ca2+ concentration in several plasma parameters (serum glucose, cholesterol and triglycerides). In conclusion, in type 1 and 2 diabetic patients we found a decrease in PMN membrane fluidity and this decrease, which was greater in type 2 diabetic patients, may be a marker of PMN dysfunction.
Caimi
G
G
Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo, Italy.
Canino
B
B
Montana
M
M
Ventimiglia
G
G
Catania
A
A
Lo Presti
R
R
D000328:Adult; D001786:Blood Glucose / Q000378:metabolism; D002118:Calcium / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D003600:Cytosol / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000031:analogs & derivatives; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008560:Membrane Fluidity; D008875:Middle Aged; D009504:Neutrophils / Q000502:physiology*; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D002118:Calcium; D002784:Cholesterol; D005456:Fluorescent Dyes; D016257:Fura-2; D006442:Glycated Hemoglobin; D014280:Triglycerides; C049925:fura-2-am