title: Thermodynamic characterization of the conformational stability of the homodimeric protein, pea lectin.
abstract: The conformational stability of the homodimeric pea lectin was determined by both isothermal urea-induced and thermal denaturation in the absence and presence of urea. The denaturation profiles were analyzed to obtain the thermodynamic parameters associated with the unfolding of the protein. The data not only conform to the simple A2 if 2U model of unfolding but also are well described by the linear extrapolation model for the nature of denaturant-protein interactions. In addition, both the conformational stability (DeltaGs) and the DeltaCp for the protein unfolding is quite high, at about 18.79 kcal/mol and 5.32 kcal/(mol K), respectively, which may be a reflection of the relatively larger size of the dimeric molecule (Mr 49 000) and, perhaps, a consequent larger buried hydrophobic core in the folded protein. The simple two-state (A2 if 2U) nature of the unfolding process, with the absence of any monomeric intermediate, suggests that the quaternary interactions alone may contribute significantly to the conformational stability of the oligomer-a point that may be general to many oligomeric proteins.
authors.affiliation: Molecular Biophysics Unit, Indian Institute of Science, Bangalore.
authors.forename: N
authors.initials: N
authors.lastname: Ahmad
authors.forename: V R
authors.initials: VR
authors.lastname: Srinivas
authors.forename: G B
authors.initials: GB
authors.lastname: Reddy
authors.forename: A
authors.initials: A
authors.lastname: Surolia
mesh_terms: D002152:Calorimetry, Differential Scanning; D019281:Dimerization; D037102:Lectins / Q000737:chemistry* / Q000378:metabolism; D018532:Pisum sativum; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013816:Thermodynamics; D014508:Urea / Q000737:chemistry / Q000378:metabolism
chemical_list: D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D014508:Urea