Exercise effects on IFN-beta expression and viral replication in lung macrophages after HSV-1 infection.
Mice exercised to fatigue and exposed to herpes simplex virus type 1 (HSV-1) exhibit greater mortality than control mice. In this study, we examined lung macrophage resistance to HSV-1 after exercise in terms of both viral replication and interferon (IFN)-beta production. We utilized the reverse transcriptase-rapid polymerase chain reaction to measure the IFN-beta mRNA content in alveolar macrophages. IFN release was measured with a bioassay, and viral replication within the macrophage was assessed by plaque titration. Exercised (Ex) mice ran on a treadmill until fatigue while control (Con) mice remained in lanes above the treadmill. After exercise, alveolar macrophages were removed and incubated with HSV-1. Alveolar macrophage IFN-beta mRNA was greater in Ex than in Con mice. Culture supernatant from infected macrophages showed a higher degree of IFN release and a higher number of infectious viral particles in Ex vs. Con mice. It is likely that the increase in IFN-beta mRNA occurs in response to a higher degree of viral replication. These results suggest that macrophages from Ex mice are less resistant to infection with HSV-1.
Kohut
M L
ML
Department of Exercise Science, School of Public Health, and Department of Microbiology/Immunology, School of Medicine, University of South Carolina, Columbia, South Carolina 29208, USA.
Davis
J M
JM
Jackson
D A
DA
Jani
P
P
Ghaffar
A
A
Mayer
E P
EP
Essig
D A
DA
D000818:Animals; D001681:Biological Assay; D006561:Herpes Simplex / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000302:isolation & purification; D016899:Interferon-beta / Q000378:metabolism*; D008168:Lung / Q000378:metabolism* / Q000473:pathology / Q000821:virology*; D016676:Macrophages, Alveolar / Q000378:metabolism / Q000821:virology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010805:Physical Conditioning, Animal; D014779:Virus Replication / Q000502:physiology*
D016899:Interferon-beta