title: Quantification of prostaglandin D synthetase in cerebrospinal fluid: a potential marker for brain tumor.
abstract: Prostaglandin D synthetase (PGD-S; prostaglandin-H2 D-isomerase, EC 5,3,99,2), a 30 kDa glycoprotein also known as beta-trace protein that catalyzes the formation of prostaglandin D2 (PGD2) from PGH2, was purified to apparent homogeneity from human cerebrospinal fluid (CSF) using a two-step procedure involving HPLC on a Vydac C8 reversed-phase column and high performance electrophoresis chromatography (HPEC) using a 10% T SDS-polyacrylamide gel. The purity of PGD-S isolated from CSF was confirmed by silver stained SDS-polyacrylamide gel and direct protein microsequencing (NH2-APEAQVSVQPNFQ). A highly specific polyclonal antibody was prepared against this protein for immunoassay development. Using an ELISA, it was found that the concentration of PGD-S in CSF did not alter significantly in different pathological conditions of the central nervous system (CNS). These include dementia (n = 9), hydrocephalus (n = 4), neuropathy (n = 11), optic neuritis (n = 4), multiple sclerosis (n = 11), and demyelinating syndrome (n = 11), when compared to normal individuals (n = 12); however, the level of PGD-S in the CSF obtained from patients with brain tumor (n = 11), was reduced by as much as 2-fold when compared to control samples (n = 12) illustrating PGD-S is a potentially useful marker for brain tumor.
authors.affiliation: Population Council, Rockefeller University, New York, New York 10021, USA.
authors.forename: L
authors.initials: L
authors.lastname: Saso
authors.forename: M G
authors.initials: MG
authors.lastname: Leone
authors.forename: C
authors.initials: C
authors.lastname: Sorrentino
authors.forename: S
authors.initials: S
authors.lastname: Giacomelli
authors.forename: B
authors.initials: B
authors.lastname: Silvestrini
authors.forename: J
authors.initials: J
authors.lastname: Grima
authors.forename: J C
authors.initials: JC
authors.lastname: Li
authors.forename: E
authors.initials: E
authors.lastname: Samy
authors.forename: D
authors.initials: D
authors.lastname: Mruk
authors.forename: C Y
authors.initials: CY
authors.lastname: Cheng
mesh_terms: D000595:Amino Acid Sequence; D000918:Antibody Specificity; D014408:Biomarkers, Tumor / Q000134:cerebrospinal fluid*; D001932:Brain Neoplasms / Q000134:cerebrospinal fluid* / Q000175:diagnosis; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000134:cerebrospinal fluid* / Q000737:chemistry / Q000302:isolation & purification; D054834:Lipocalins; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid
chemical_list: D014408:Biomarkers, Tumor; D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase