title: Extraction and quantification of nicardipine in human plasma.
abstract: A novel simple method of extraction, separation, identification and quantification of nicardipine in human plasma samples was completely studied. The human plasma samples were initially purified by solid-phase extraction (SPE) using a C18 cartridge. The extracted samples were separated and nicardipine present in the samples was quantified by high-performance liquid chromatography (HPLC) on a reversed-phase C18 column employing a mobile phase consisting of 60% (v/v) acetonitrile in 0.02 M NaH2PO4 with pH of 6.3 and a variable wavelength UV detector set at 254 nm. The recovery of nicardipine from plasma samples using selective SPE was 91+/-6.0% and had less interfering compounds in the HPLC analysis compared to the use of liquid-liquid (L/L) extraction. In the HPLC analysis, examining the effect of pH values of the mobile phase on the capacity factor (k') of nicardipine revealed a method for selecting a critical k' value of nicardipine to eliminate interfering peaks near the peak specific to the analyte. This method for quantification of nicardipine in human plasma samples was suitable for studying the pharmacokinetic profile of nicardipine administered as an intravenous bolus to cardiac surgical patients.
authors.affiliation: Center for Research in Anesthesia, Department of Anesthesia, University of Pennsylvania Health System, Philadelphia 19104, USA.
authors.forename: Q C
authors.initials: QC
authors.lastname: Meng
authors.forename: A T
authors.initials: AT
authors.lastname: Cheung
authors.forename: D
authors.initials: D
authors.lastname: Guvakov
authors.forename: S J
authors.initials: SJ
authors.lastname: Weiss
authors.forename: J S
authors.initials: JS
authors.lastname: Savino
authors.forename: I S
authors.initials: IS
authors.lastname: Salgo
authors.forename: B E
authors.initials: BE
authors.lastname: Marshall
mesh_terms: D000959:Antihypertensive Agents / Q000097:blood* / Q000493:pharmacokinetics; D002121:Calcium Channel Blockers / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009529:Nicardipine / Q000097:blood* / Q000493:pharmacokinetics
chemical_list: D000959:Antihypertensive Agents; D002121:Calcium Channel Blockers; D009529:Nicardipine