title: Role of cytokines in the maintenance of the memory immune response induced by a rat histiocytoma in syngeneic hosts.
abstract: The nature of immune memory induced by a rat histiocytoma, AK-5, in syngeneic hosts was studied. AK-5 tumor when transplanted intraperitoneally (i.p.) into naive animals grows as ascites and is 100% fatal. However, spontaneous regression of AK-5 tumor was observed in 60% of animals upon s.c. transplantation. Interestingly, all the tumor-rejected animals (immune) were found to resist further i.p. challenges with AK-5 cells. The immunity thus developed is specific for AK-5 tumor, since other tumors grow in these animals. In order to understand the tumor-specific immune memory induced after AK-5 tumor transplantation, we have evaluated circulatory-cytokine profiles of i.p. tumor-transplanted naive and immune animals. Our results show an increase in the levels of IL-2, IL-12 and IL-4 in tumor-injected immune animals compared with normal animals, whereas the interferon-gamma levels were totally reversed in these two sets of animals. We also found elevated levels of circulating immune complexes in the sera from AK-5-rechallenged immune animals. We have also evaluated the cytotoxic potential of splenocytes and pure natural killer cells from immune animals rechallenged with AK-5 cells, and have found a significant increase in antibody-dependent cellular cytotoxicity. Similarly, in vitro proliferation of total splenocytes and nylon-wool non-adherent cells from immune animals was much higher compared with the normal animals. The present study thus suggests antigen-independent maintenance of clonal burst size, which could be the form of immune memory induced by AK-5 tumor in the syngeneic host.
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: K S
authors.initials: KS
authors.lastname: Nandakumar
authors.forename: C
authors.initials: C
authors.lastname: Varalakshmi
authors.forename: A
authors.initials: A
authors.lastname: Khar
mesh_terms: D000818:Animals; D016207:Cytokines / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D018219:Histiocytoma, Benign Fibrous / Q000276:immunology*; D007156:Immunologic Memory; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar; D012878:Skin Neoplasms / Q000276:immunology*
chemical_list: D016207:Cytokines