title: Platelet-like particle production from cultured human megakaryocytic cell line.
abstract: The human megakaryocytic cell line CMK was positive for the expression of platelet glycoprotein(GP) IIb/IIIa but few cells were positive for GPIb. To establish a GPIb-positive CMK subclone, GPIb-positive CMK cells were purified, micromanipulated and cloned, but resulting sublines were all negative for GPIb expression. The combined presence of interleukin-3 (IL-3) and the anti-virus agent ribavirin strongly induced expression of GPIb on the cells. However, the megakaryocyte growth and development factor (MGDF)/thrombopoietin (TPO) failed to enhance GPIb expression. CMK themselves and particles are negative for GPIb expression. To utilize these particles as platelet substitutes, further appropriate culture systems are necessary.
authors.affiliation: Hokkaido Red Cross Blood Center, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Ikebuchi
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: K
authors.initials: K
authors.lastname: Niwa
authors.forename: H
authors.initials: H
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Fujihara
authors.forename: S
authors.initials: S
authors.lastname: Sekiguchi
mesh_terms: D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001802:Blood Substitutes / Q000378:metabolism*; D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans; D016130:Immunophenotyping; D008533:Megakaryocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
chemical_list: D001802:Blood Substitutes