title: Detection of Chlamydia trachomatis antibodies by 2 novel tests: rELISA and peptide EIA.
abstract: The performance of 2 newly developed enzyme immunoassays (EIAs) intended for the routine serological diagnosis of chlamydial infections was evaluated. rELISA is based on a recombinant lipopolysaccharide antigen which detects chlamydia genus-specific antibodies, and Chlamydia trachomatis EIA is based on a peptide derived from major outer membrane protein and is therefore species-specific. Both tests distinguished patients with tubal factor infertility (TFI) or pelvic inflammatory disease (PID) from the controls. The prevalence of IgA antibodies was higher for the PID patients than for the TFI patients; the finding indicates a more active state of infections for the PID patients. Furthermore, C. trachomatis EIA detected more IgG antibodies in the TFI patients than in patients with non-tubal factor infertility. In conclusion, rELISA detected chlamydial antibodies in general, and C. trachomatis EIA detected species-specific antibodies. These EIA tests may be useful in the serodiagnosis of chlamydial infection.
authors.affiliation: Department of Obstetrics and Gynecology, University of Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Paukku
authors.forename: A
authors.initials: A
authors.lastname: Närvänen
authors.forename: M
authors.initials: M
authors.lastname: Puolakkainen
authors.forename: K
authors.initials: K
authors.lastname: Dreesbach
authors.forename: A
authors.initials: A
authors.lastname: Tiitinen
authors.forename: W
authors.initials: W
authors.lastname: Hao
authors.forename: T I
authors.initials: TI
authors.lastname: Anttila
authors.forename: J
authors.initials: J
authors.lastname: Paavonen
mesh_terms: D000328:Adult; D000907:Antibodies, Bacterial / Q000097:blood*; D002690:Chlamydia Infections / Q000453:epidemiology / Q000276:immunology / Q000382:microbiology*; D002692:Chlamydia trachomatis / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D010455:Peptides / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D010455:Peptides