title: Tympano-ossicular allografts and HIV transmission.
abstract: OBJECTIVE
  To evaluate the potential risk of human immunodeficiency virus (HIV) transmission by tympano-ossicular allografts by studying the efficacy of standard preservation techniques to eliminate the presence of proviral HIV-1 DNA fragments in contaminated ossicles.
  
  
  METHODS
  Randomized single-blind prospective study on the ossicles of HIV-1 patients.
  
  
  METHODS
  Ossicles of five patients who had died of acquired immune deficiency syndrome (AIDS) (HIV-1 infection) were taken within 6 hours postmortem and allocated randomly to a treatment and nontreatment group. Liver and skin biopsies were taken as positive control specimens. PROCESSING: The treatment group was processed with standard techniques (formaldehyde) for tympano-ossicular allograft preservation and the nontreatment group was only washed, dried, and stored in sterile tubes at -700 degrees without further processing.
  
  
  METHODS
  Proviral HIV-1 DNA was detected using polymerase chain reaction amplification techniques.
  
  
  RESULTS
  No proviral HIV-1 DNA was detected in any of the treated ossicles, whereas three of five sets of untreated ossicles were positive. The positive control specimens of all treated and nontreated sets were positive for proviral HIV-1 DNA.
  
  
  CONCLUSIONS
  These results suggest that the preservation technique for tympano-ossicular allografts is safe with regard to HIV-1 transmission.
authors.affiliation: University Department Otolaryngology, St-Augustinus Hospital, Antwerp-Wilrijk, Belgium.
authors.forename: S
authors.initials: S
authors.lastname: Janssens de Varebeke
authors.forename: P
authors.initials: P
authors.lastname: Goubau
authors.forename: H K
authors.initials: HK
authors.lastname: Beuselinck
authors.forename: T h
authors.initials: Th
authors.lastname: Somers
authors.forename: F E
authors.initials: FE
authors.lastname: Offeciers
authors.forename: P J
authors.initials: PJ
authors.lastname: Govaerts
mesh_terms: D004279:DNA, Viral / Q000032:analysis; D004202:Disinfectants; D004429:Ear Ossicles / Q000637:transplantation* / Q000821:virology*; D005557:Formaldehyde; D015658:HIV Infections / Q000517:prevention & control* / Q000635:transmission*; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D017053:Infection Control / Q000379:methods; D009926:Organ Preservation / Q000379:methods*; D011446:Prospective Studies; D016037:Single-Blind Method; D014184:Transplantation, Homologous / Q000009:adverse effects*; D014432:Tympanic Membrane / Q000637:transplantation* / Q000821:virology*
chemical_list: D004279:DNA, Viral; D004202:Disinfectants; D005557:Formaldehyde