title: The Pur10 protein encoded in the gene cluster for puromycin biosynthesis of Streptomyces alboniger is an NAD-dependent ATP dehydrogenase.
abstract: The pur10 gene of the puromycin (pur) cluster of Streptomyces alboniger is essential for the biosynthesis of this antibiotic. Highly purified Pur10 protein, obtained in Escherichia coli as a recombinant protein fused to a histidine tail, had an NAD-dependent ATP dehydrogenase activity. The Km and Vmax values for ATP were 0.49 mM and 14.5 nmol/min and for NAD 0.53 mM and 15.2 nmol/min, respectively. The ATP-derived product of the reaction apparently decomposed producing a triphosphorylated compound plus an adenine derivative. These and previous results suggested that Pur10 carries out the first step of the puromycin biosynthetic pathway, namely, conversion of ATP into 3'-keto-3'-deoxyATP.
authors.affiliation: Centro de Biologia Molecular Severo Ochoa (CSIC/UAM), Universidad Autónoma, Cantoblanco, Madrid, Spain.
authors.forename: M A
authors.initials: MA
authors.lastname: Rubio
authors.forename: J C
authors.initials: JC
authors.lastname: Espinosa
authors.forename: J A
authors.initials: JA
authors.lastname: Tercero
authors.forename: A
authors.initials: A
authors.lastname: Jiménez
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000429:Alcohol Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins; D002384:Catalysis; D004789:Enzyme Activation / Q000235:genetics; D005798:Genes, Bacterial; D020043:Genes, Essential; D007700:Kinetics; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D009243:NAD / Q000378:metabolism*; D010088:Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011691:Puromycin / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D009243:NAD; D011691:Puromycin; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases; C115699:Pur10 protein, Streptomyces alboniger