title: Construction of biosensors using a gold-binding polypeptide and a miniature integrated surface plasmon resonance sensor.
abstract: Surface plasmon resonance (SPR) biosensors were constructed on miniature integrated sensors. Recognition elements were attached to the sensor surface using a gold-binding repeating polypeptide. Biosensors with fluorescyl groups attached to their surfaces were functional for at least 1 month of daily use with little decrease in response to the binding of an anti-fluorescyl monoclonal antibody. The coupling of protein A to the gold-binding polypeptide on the sensor surface enabled the biosensor to detect the binding of antibodies to the protein A and provided a sensor with convertible specificity. The system described herein provides a simple and rapid approach for the fabrication of highly specific, durable, portable and low cost SPR-based biosensors.
authors.affiliation: Department of Medicine, University of Washington, Seattle 98195, USA.
authors.forename: R G
authors.initials: RG
authors.lastname: Woodbury
authors.forename: C
authors.initials: C
authors.lastname: Wendin
authors.forename: J
authors.initials: J
authors.lastname: Clendenning
authors.forename: J
authors.initials: J
authors.lastname: Melendez
authors.forename: J
authors.initials: J
authors.lastname: Elkind
authors.forename: D
authors.initials: D
authors.lastname: Bartholomew
authors.forename: S
authors.initials: S
authors.lastname: Brown
authors.forename: C E
authors.initials: CE
authors.lastname: Furlong
mesh_terms: D000595:Amino Acid Sequence; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000737:chemistry*; D020349:Surface Plasmon Resonance
chemical_list: D013205:Staphylococcal Protein A; D006046:Gold