Effects of a gonadotropin-releasing hormone agonist on rat ovarian adenocarcinoma cell lines in vitro and in vivo.
To evaluate the biologic effects of the gonadotropin-releasing hormone (GnRH) agonist buserelin on rat ovarian adenocarcinoma cells in vivo and in vitro, female Wistar rats with primary ovarian adenocarcinoma induced by 7, 12-dimethylbenz(a)anthracene (DMBA) and the DMBA-OC-1 cell line established from a DMBA-induced rat tumor were used in this study. In vivo, daily administration of buserelin significantly suppressed the release of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and progesterone as compared with controls. Buserelin did not inhibit the growth of DMBA-induced tumors. However, histopathologically, there was increased central necrosis and a decrease in the number of neoplastic cells, with proliferation of connective tissue, in the group treated with buserelin. In vitro, FSH-induced proliferation of DMBA-OC-1 cells was suppressed by buserelin. Thus, this basic experimental study supports the potential use of a GnRH agonist to suppress the growth of ovarian cancer.
Maruuchi
T
T
Department of Obstetrics and Gynecology, Kurume University School of Medicine, Fukuoka.
Sugiyama
T
T
Kataoka
A
A
Nishida
T
T
Yakushiji
M
M
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma / Q000139:chemically induced / Q000503:physiopathology*; D000818:Animals; D002064:Buserelin / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000139:chemically induced / Q000503:physiopathology*; D011374:Progesterone / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014407:Tumor Cells, Cultured
D006063:Chorionic Gonadotropin; D011374:Progesterone; D015127:9,10-Dimethyl-1,2-benzanthracene; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D002064:Buserelin