Chromosome translocations detected by fluorescence in situ hybridisation (FISH)--a useful tool in population monitoring?
It is assumed that symmetrical translocations persist in peripheral lymphocytes for years. For this reason, they should be valuable indicators especially for chronic exposures of populations to mutagens or carcinogens. Reciprocal translocations can be easily detected by means of fluorescence in situ hybridization (FISH). Very little is known about the spontaneous frequency, which forms the basis for population monitoring, and the variations which exist between individuals. The frequencies obtained on the basis of structural chromosome aberrations detected by means of FISH in 29 healthy persons are presented, and the deviations in the occurrence of symmetrical translocations arising in the different persons are indicated. Tests for the determination of persistence levels, are carried out on persons exposed to radiation. The chromosomes 2, 4 and 8 are painted parallel to the centromeres, by means of the FISH technique.
Pressl
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S
Institute for Radiation Hygiene, Federal Office for Radiation Protection, Oberschleissheim, Germany.
Stephan
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G
D000328:Adult; D000368:Aged; D002877:Chromosomes, Human / Q000528:radiation effects; D004247:DNA / Q000235:genetics / Q000528:radiation effects; D004784:Environmental Monitoring / Q000379:methods*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure; D014178:Translocation, Genetic
D004247:DNA