title: Methanoculleus palmolei sp. nov., an irregularly coccoid methanogen from an anaerobic digester treating wastewater of a palm oil plant in north-Sumatra, Indonesia.
abstract: Strain INSLUZT (= DSM 4273T) was isolated from a biogas-producing bioreactor treating wastewater of a palm oil mill on North-Sumatra (Indonesia). Cells of strain INSLUZT were highly irregularly coccoid, 1.25-2.0 microns in diameter, had a cell envelope consisting of the cytoplasmic membrane and an S-layer of hexagonally arranged glycoprotein subunits with an M(r) of 120,000, and were flagellated (motility was not observed). Cells were mesophilic and grew most rapidly at 40 degrees C on H2/CO2' formate, 2-propanol/CO2 2-butanol/CO2 and cyclopentanol/CO2 to give methane. Tungstate promoted growth on H2/CO2 with acetate as the solely required organic medium supplement. The G + C content of DNA was 59 mol% (Tm method) and 59.5 mol% (HPLC method). 16S rDNA analysis revealed a phylogenetic relationship to Methanoculleus species; the name Methanoculleus palmolei sp. nov. is therefore proposed for strain INSLUZT (= DSM 4273T).
authors.affiliation: GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Hydrologie, Neuherberg, Germany. gerhard.zellner@gsf.de
authors.forename: G
authors.initials: G
authors.lastname: Zellner
authors.forename: P
authors.initials: P
authors.lastname: Messner
authors.forename: J
authors.initials: J
authors.lastname: Winter
authors.forename: E
authors.initials: E
authors.lastname: Stackebrandt
mesh_terms: D000693:Anaerobiosis; D001482:Base Composition; D001673:Biodegradation, Environmental; D019149:Bioreactors; D019641:DNA, Archaeal / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D007214:Indonesia; D007220:Industrial Waste; D008562:Membrane Glycoproteins / Q000737:chemistry; D008697:Methane / Q000378:metabolism; D019638:Methanomicrobiaceae / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D010938:Plant Oils; D011073:Polyamines / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D009626:Terminology as Topic; D014877:Water Pollution, Chemical
chemical_list: D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D007220:Industrial Waste; D008562:Membrane Glycoproteins; D010938:Plant Oils; D011073:Polyamines; D012336:RNA, Ribosomal, 16S; D008697:Methane