Assessment of chromosomal heterogeneity in tumoral cell lines using PRINS technique.
The primed in situ (PRINS) labeling technique has been used for the interphase cytogenetic investigation of 3 colon cancer cell lines (Caco-2, TC7 and PF1 1) derived from a same primary tumor. A panel of 10 chromosome-specific primers (for chromosomes 1, 2, 3, 7, 8, 9, 10, 13, 16 and 18) has been utilized in simple and double color PRINS reactions. Each cell line displayed a heterogeneous distribution of copy number for several chromosomes. The karyotypic heterogeneity was also significant between the 3 cell lines. These data indicate the common occurrence of chromosome heterogeneity in tumoral cell lines and demonstrate the feasibility of interphase PRINS procedure for analysis of numerical changes in tumoral cells.
Pellestor
F
F
CNRS UPR 1142, I.G.H., Montpellier, France.
Andreo
B
B
Coullin
P
P
D002877:Chromosomes, Human; D003110:Colonic Neoplasms / Q000235:genetics*; D005069:Evaluation Studies as Topic; D018740:Genetic Heterogeneity; D006801:Humans; D007621:Karyotyping; D020401:Primed In Situ Labeling; D014407:Tumor Cells, Cultured