title: Effects of aspirin on arylamine N-acetyltransferase activity in Klebsiella pneumoniae.
abstract: This study was designed to assess the effects of aspirin on arylamine N-acetyltransferase (NAT) activities in the bacterium Klebsiella pneumoniae using high performance liquid chromatography to measure the acetylation of 2-aminofluorene (2-AF) with or without aspirin. Cytosols or suspensions of K. pneumoniae with or without specific concentrations of aspirin co-treatment showed different percentages of 2-AF acetylation. The data indicated that there was decreased NAT activity associated with increased levels of aspirin in K. pneumoniae cytosols and in intact bacteria. For the cytosol examination, the apparent values of Km and Vmax decreased 0.59- and 0.58-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. For the intact bacteria examination, the apparent values of Km and Vmax decreased 0.60- and 0.67-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. This report is the first demonstration to show that aspirin can decrease N-acetyltransferase activity in the bacterium K. pneumoniae.
authors.affiliation: Department of Microbiology, China Medical College, Taiwan, Republic of China.
authors.forename: J G
authors.initials: JG
authors.lastname: Chung
authors.forename: T W
authors.initials: TW
authors.lastname: Tan
authors.forename: H Y
authors.initials: HY
authors.lastname: Tsai
authors.forename: W T
authors.initials: WT
authors.lastname: Hsieh
authors.forename: G W
authors.initials: GW
authors.lastname: Chen
authors.forename: J M
authors.initials: JM
authors.lastname: Lai
mesh_terms: D000107:Acetylation / Q000187:drug effects; D001191:Arylamine N-Acetyltransferase / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003600:Cytosol / Q000378:metabolism; D005449:Fluorenes / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology*; D013997:Time Factors
chemical_list: D005449:Fluorenes; C012177:2-aminofluorene; D001191:Arylamine N-Acetyltransferase; D001241:Aspirin