Effect of extracellular palmitate on 2-deoxy-d-glucose uptake in muscle from Ad libitum fed and calorie restricted rats.
We studied the effect of a high physiologic concentration of palmitate (1mM) on in vitro 2-deoxy-D-glucose (2DG) uptake by flexor digitorum brevis (FDB) muscle from ad libitum fed rats (AL) and rats fed 60% of ad libitum intake (CR) for 20 days. CR did not alter muscle 2DG uptake in the absence of insulin, but relative to AL, CR significantly (p<0.01) increased 2DG uptake in the presence of 20,000 microU/ml insulin. This effect of CR persisted in the presence of 1mM palmitate. The presence of 1mM palmitate significantly (p<0.01) impaired 2DG glucose uptake, both in the presence and absence of insulin, to the same extent in AL and CR muscle, despite an 18% decrease in FABPpm expression with CR. Thus, although CR profoundly affects insulin-mediated muscle glucose uptake, it does not alter the ability of extracellular fatty acid to modulate glucose utilization by skeletal muscle.
Gazdag
A C
AC
Department of Kinesiology and Department of Nutritional Sciences, University of Wisconsin, Madison, USA.
Tucker
M Z
MZ
Turcotte
L P
LP
Dean
D J
DJ
Cartee
G D
GD
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D002352:Carrier Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003847:Deoxyglucose / Q000493:pharmacokinetics*; D002149:Energy Intake; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000494:pharmacology; D005508:Food Deprivation; D005947:Glucose / Q000493:pharmacokinetics; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D018996:Myelin P2 Protein / Q000378:metabolism; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010168:Palmitates / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344
D001786:Blood Glucose; D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005230:Fatty Acids, Nonesterified; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D010168:Palmitates; D003847:Deoxyglucose; D005947:Glucose