[Cloning and expression of thermostable secretory metalloproteinase genes from two Bacillus brevis strains in Bacillus subtilis cells].
Two closely related genes of thermostable Bac. brevis metalloproteases were cloned and expressed in Bac. subtilis cells. Their restriction maps and directions of transcription were determined. Thermostability and thermal optimum of proteolytic activity of cloned gene products are significantly lower than those of native enzymes. The authors believe that alteration of the enzymes' characteristics may be due to uncorrected folding of thermostable protein in case of its expression in mesophilic bacterial strains.
Akimkina
T V
TV
De Medeiros Silva
G
G
Iaroshevskaia
E M
EM
Nosovskaia
E A
EA
Tsaplina
I A
IA
Karavaĭko
G I
GI
Kostrov
S V
SV
D001407:Bacillus / Q000235:genetics*; D001412:Bacillus subtilis / Q000235:genetics*; D003001:Cloning, Molecular; D004795:Enzyme Stability; D015870:Gene Expression; D005798:Genes, Bacterial; D008666:Metalloendopeptidases / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D013696:Temperature
D011994:Recombinant Proteins; D008666:Metalloendopeptidases; C044194:microbial metalloproteinases
Klonirovanie i ékspressia genov termostabil'nykh sekretornykh metalloproteinaz dvukh shtammov Bacillus brevis v kletkakh Bacillus Subtilis.