Fringe is essential for mirror symmetry and morphogenesis in the Drosophila eye.
An early event in Drosophila eye development is the division of the eye disc into dorsoventral domains. The dorsoventral pattern is displayed in the adult compound eye as a distinct mirror symmetry across the dorsoventral midline or equator. The dorsoventral axis is also implicated in organizing early development of the eye, as retinal differentiation is initiated at the posterior dorsoventral midline. Here we show that Fringe is expressed specifically in the ventral half of the undifferentiated eye disc, thus creating a dorsoventral boundary. Ectopic Fringe borders that are generated by clones of fringe cells can reverse the planar polarity of photoreceptor clusters, indicating that the Fringe boundary is crucial for the induction of mirror symmetry. Lack of a Fringe boundary disrupts equatorial expression of Notch signalling proteins and causes a complete failure of eye development. Our results indicate that the formation of the Fringe boundary and subsequent Notch signalling at the equator are essential for organizing mirror symmetry and eye morphogenesis.
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Department of Cell Biology, Program of Developmental Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
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D000818:Animals; D019521:Body Patterning / Q000502:physiology; D016764:Cell Polarity; D004330:Drosophila / Q000196:embryology* / Q000235:genetics; D029721:Drosophila Proteins; D004627:Embryonic Induction / Q000502:physiology; D005123:Eye / Q000196:embryology; D005260:Female; D019476:Insect Proteins / Q000235:genetics / Q000502:physiology*; D008297:Male; D008565:Membrane Proteins / Q000502:physiology; D009024:Morphogenesis / Q000502:physiology*; D017351:N-Acetylglucosaminyltransferases; D017956:Photoreceptor Cells, Invertebrate / Q000196:embryology* / Q000502:physiology; D051880:Receptors, Notch; D015398:Signal Transduction; D013696:Temperature
D029721:Drosophila Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C497759:N protein, Drosophila; D051880:Receptors, Notch; D017351:N-Acetylglucosaminyltransferases; C090576:fng protein, Drosophila