Adenoviral-mediated gene transfer to human adipocytes in vitro, and human adipose tissue ex vivo and rabbit femoral adipose tissue in vivo.
Adenoviral-mediated gene transfer has proven useful in several organ systems to understand gene action and to provide a potential therapeutic modality for localized, organ-specific gene overexpression. However, the application of adenoviral-mediated gene transfer to adipocytes and adipose tissue has not been evaluated. We evaluated the feasibility of in vitro and ex vivo transfer of the beta-galactosidase gene to human adipocytes and adipose tissue by means of adenoviral vectors. The efficiency (percentage of cells transduced) of adenoviral-mediated gene transfer of the beta-galactosidase gene to human adipocytes in vitro and to human adipose tissue ex vivo was 21 +/- 3% and 14 +/- 3%, respectively. Adenoviral-mediated gene transfer in a rabbit femoral adipose tissue was also demonstrated in vivo. Adenoviral-mediated gene transfer may facilitate studies on understanding the biology of adipocytes and provide a potential tool for the modulation of adipocyte function in vivo and thereby for the treatment of obesity.
Levine
J A
JA
Department of Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA. Levine.james@Mayo.edu
Eberhardt
N L
NL
Jensen
M D
MD
O'Brien
T
T
D000256:Adenoviridae / Q000235:genetics*; D017667:Adipocytes / Q000737:chemistry*; D000273:Adipose Tissue / Q000737:chemistry*; D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008297:Male; D011817:Rabbits; D001616:beta-Galactosidase / Q000737:chemistry / Q000235:genetics*
D001616:beta-Galactosidase