Rapid PCR detection of Helicobacter pylori-associated virulence and resistance genes directly from gastric biopsy material.
We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.
Björkholm
B
B
Department of Medicine, Karolinska Institute, Stockholm, Sweden.
Befrits
R
R
Jaup
B
B
Engstrand
L
L
D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D017291:Clarithromycin / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000235:genetics; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D006801:Humans; D016133:Polymerase Chain Reaction; D013270:Stomach / Q000382:microbiology*; D014774:Virulence
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori; D017291:Clarithromycin