Development of an immunoassay for monitoring the levels of ciprofloxacin in patient samples.
It has been traditional to measure drug concentrations using high pressure liquid chromatography (HPLC). While this method is highly accurate, it is time consuming and requires the use of appropriate standards for identification of the compound. In addition, identification and quantification of drugs from patient samples requires significant manipulation to remove protein. In contrast, enzyme-linked immunoassays (EIA) are able to assay samples with a high degree of specificity, and are able to process multiple samples at a time. In addition, serum proteins do not interfere with sample quantification and samples may be tested without significant preparation. We describe the development of an EIA for the detection of ciprofloxacin in serum and dialysate samples. The immunoassay is specific for ciprofloxacin and is sensitive for picogram amounts of the antibiotic.
Snitkoff
G G
GG
Albany College of Pharmacy, Department of Biological Sciences, New York 12208, USA.
Grabe
D W
DW
Holt
R
R
Bailie
G R
GR
D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D001667:Binding, Competitive / Q000276:immunology; D001798:Blood Proteins / Q000378:metabolism; D002939:Ciprofloxacin / Q000097:blood* / Q000378:metabolism; D006801:Humans; D007114:Immunization; D007124:Immunoenzyme Techniques / Q000379:methods*; D011485:Protein Binding; D011817:Rabbits; D017186:Titrimetry
D001798:Blood Proteins; D002939:Ciprofloxacin