title: Effect of serial passage in tissue culture on sequence of omp1 from Chlamydia trachomatis clinical isolates.
abstract: Previous epidemiological studies of Chlamydia trachomatis frequently have required expansion of isolates in tissue culture. The possibility that C. trachomatis omp1 might undergo mutation during such expansion has not been examined systematically. We found no differences in the omp1 sequences from 10 clinical specimens before and after 20 in vitro passages.
authors.affiliation: Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana 46202, USA. dstothar@iupui.edu
authors.forename: D R
authors.initials: DR
authors.lastname: Stothard
authors.forename: B
authors.initials: B
authors.lastname: Van Der Pol
authors.forename: N J
authors.initials: NJ
authors.lastname: Smith
authors.forename: R B
authors.initials: RB
authors.lastname: Jones
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D001483:Base Sequence; D002692:Chlamydia trachomatis / Q000235:genetics*; D046508:Culture Techniques; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D018272:Porins
chemical_list: D001425:Bacterial Outer Membrane Proteins; D018272:Porins; C071677:omp1 protein, Chlamydia trachomatis