Construction of biosensors using a gold-binding polypeptide and a miniature integrated surface plasmon resonance sensor.
Surface plasmon resonance (SPR) biosensors were constructed on miniature integrated sensors. Recognition elements were attached to the sensor surface using a gold-binding repeating polypeptide. Biosensors with fluorescyl groups attached to their surfaces were functional for at least 1 month of daily use with little decrease in response to the binding of an anti-fluorescyl monoclonal antibody. The coupling of protein A to the gold-binding polypeptide on the sensor surface enabled the biosensor to detect the binding of antibodies to the protein A and provided a sensor with convertible specificity. The system described herein provides a simple and rapid approach for the fabrication of highly specific, durable, portable and low cost SPR-based biosensors.
Woodbury
R G
RG
Department of Medicine, University of Washington, Seattle 98195, USA.
Wendin
C
C
Clendenning
J
J
Melendez
J
J
Elkind
J
J
Bartholomew
D
D
Brown
S
S
Furlong
C E
CE
D000595:Amino Acid Sequence; D006046:Gold / Q000737:chemistry*; D008839:Microelectrodes; D008969:Molecular Sequence Data; D011485:Protein Binding; D013205:Staphylococcal Protein A / Q000737:chemistry*; D020349:Surface Plasmon Resonance
D013205:Staphylococcal Protein A; D006046:Gold