title: Experimental infection in cats with a cagA+ human isolate of Helicobacter pylori.
abstract: BACKGROUND
  Helicobacter pylori has been cultured from the inflamed gastric mucosa of naturally and experimentally-infected cats. The lesions in the H. pylori-infected cat stomach mimic many of the features seen in human stomachs infected with H. pylori. This study sought to determine whether H. pylori-negative, specific pathogen-free cats with normal gastric mucosa were susceptible to colonization with a human cagA+ strain of H. pylori, and whether gastritis developed after infections.
  
  
  METHODS
  Four H. pylori-negative cats treated with cimetidine were orally dosed 3 times at 2-day intervals with 3 ml (1.5 x 108 CFU/ml) of H. pylori.
  
  
  RESULTS
  All experimentally-infected cats became persistently colonized as determined by H. pylori isolation from gastric tissue by culture at 12 weeks, and all 4 cats were found positive by PCR during serial gastric biopsies and necropsy at 15 weeks postinoculation. The 2 control cats did not have H. pylori isolated, nor was gastric tissue positive by PCR. The H. pylori isolated from the 4 experimentally-infected cats had RFLP patterns specific for the flaA gene identical to those of the inoculating strain. All 4 H. pylori-infected cats had multifocal gastritis, consisting of lymphoid aggregates plus multiple large lymphoid nodules. In the control cats, one cat had a few focal lymphocytic aggregates in the body submucosa, whereas the second cat had normal gastric mucosa.
  
  
  CONCLUSIONS
  Human cagA+ H. pylori readily colonized the cat stomach and produced a persistent gastritis. The findings demonstrate the utility of the cat to study H. pylori induced pathogenesis.
authors.affiliation: Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Perkins
authors.forename: J G
authors.initials: JG
authors.lastname: Fox
authors.forename: R P
authors.initials: RP
authors.lastname: Marini
authors.forename: Z
authors.initials: Z
authors.lastname: Shen
authors.forename: C A
authors.initials: CA
authors.lastname: Dangler
authors.forename: Z
authors.initials: Z
authors.lastname: Ge
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000235:genetics*; D002415:Cats; D005260:Female; D005756:Gastritis / Q000382:microbiology / Q000473:pathology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori / Q000235:genetics* / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C080866:cagA protein, Helicobacter pylori