title: Mucosal metabolism in ulcerative colitis and Crohn's disease.
abstract: OBJECTIVE
  Colonic mucosal metabolism of butyrate may be impaired in ulcerative colitis. In this study we sought to confirm this observation, to determine if a similar change occurs in Crohn's colitis, and to establish whether a panenteric disorder of butyrate metabolism exists in either condition.
  
  
  METHODS
  With use of a microculture technique, mucosal metabolic fluxes of 14[C]-labeled butyrate and 14[C]-labeled glutamine were measured as 14[C] carbon dioxide production in mucosal biopsy specimens from the colon and ileum in patients with ulcerative colitis, Crohn's colitis, and healthy bowel. Results were expressed as pmol/microg biopsy DNA/hour.
  
  
  RESULTS
  In the colon the mucosal metabolic fluxes of both butyrate and glutamine are reduced in both ulcerative colitis and Crohn's colitis compared with healthy controls. These changes were most marked in the presence of moderate to severe mucosal inflammation, there being no significant difference in mucosal metabolic flux between mildly inflamed mucosa and healthy controls. In the ileum the mucosal metabolic fluxes of butyrate and glutamine did not differ between healthy controls and those with either ulcerative colitis or Crohn's colitis.
  
  
  CONCLUSIONS
  Changes in colonic mucosal metabolism of butyrate and glutamine in inflammatory bowel disease occur as a consequence of the inflammatory process and are not peculiar to ulcerative colitis. Ileal mucosal metabolism is unchanged in ulcerative colitis and Crohn's colitis, indicating the absence of a panenteric abnormality of mucosal metabolism in these two conditions.
authors.affiliation: Department of Surgery, Mater Misericordiae Hospital and University College, Dublin, Ireland.
authors.forename: M M
authors.initials: MM
authors.lastname: Duffy
authors.forename: M C
authors.initials: MC
authors.lastname: Regan
authors.forename: P
authors.initials: P
authors.lastname: Ravichandran
authors.forename: C
authors.initials: C
authors.lastname: O'Keane
authors.forename: M G
authors.initials: MG
authors.lastname: Harrington
authors.forename: J M
authors.initials: JM
authors.lastname: Fitzpatrick
authors.forename: P R
authors.initials: PR
authors.lastname: O'Connell
mesh_terms: D001706:Biopsy; D002087:Butyrates / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D003424:Crohn Disease / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*
chemical_list: D002087:Butyrates; D005973:Glutamine