title: Binding of CDK9 to TRAF2.
abstract: CDK9 has been recently shown to have increased kinase activity in differentiated cells in culture and a differentiated tissue-specific expression in the developing mouse. In order to identify factors that contribute to CDK9's differentiation-specific function, we screened a mouse embryonic library in the yeast two-hybrid system and found a tumor necrosis factor signal transducer, TRAF2, to be an interacting protein. CDK9 interacts with a conserved domain in the TRAF-C region of TRAF2, a motif that is known to bind other kinases involved in TRAF-mediated signaling. Endogenous interaction between the two proteins appears to be specific to differentiated tissue. TRAF2-mediated signaling may incorporate additional kinases to signal cell survival in myotubes, a cell type that is severely affected in TRAF2 knockout mice.
authors.affiliation: Sbarro Institute for Cancer Research and Molecular Medicine, Department of Pathology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
authors.forename: T K
authors.initials: TK
authors.lastname: MacLachlan
authors.forename: N
authors.initials: N
authors.lastname: Sang
authors.forename: A
authors.initials: A
authors.lastname: De Luca
authors.forename: P L
authors.initials: PL
authors.lastname: Puri
authors.forename: M
authors.initials: M
authors.lastname: Levrero
authors.forename: A
authors.initials: A
authors.lastname: Giordano
mesh_terms: D000818:Animals; D001665:Binding Sites; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D042863:Cyclin-Dependent Kinase 9; D006801:Humans; D051379:Mice; D018482:Muscle, Skeletal / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013347:Subcellular Fractions; D047992:TNF Receptor-Associated Factor 2; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016328:NF-kappa B; D011506:Proteins; D011994:Recombinant Proteins; D047992:TNF Receptor-Associated Factor 2; D014409:Tumor Necrosis Factor-alpha; D011494:Protein Kinases; C467969:CDK9 protein, human; C467972:Cdk9 protein, mouse; D042863:Cyclin-Dependent Kinase 9