title: Adenylyl cyclase type 7 is the predominant isoform in the bovine retinal pigment epithelium.
abstract: The retinal pigment epithelium (RPE) fulfills important supporting tasks to maintain the visual functions of the sensorineural retina. One major signalling mechanism by which adjacent tissues impinge on the RPE is the adenylyl cyclase (AC)/cAMP pathway. In the RPE, cAMP seems to modulate unique functions such as the phagocytosis of discs shed from the rod outer segments, transport of vitamin A or the ion and fluid control in the subretinal space. We analyzed the AC expression pattern in the retina and the RPE and found AC type 7 to be almost the only isoform expressed in the RPE. We cloned AC type 7 from a cDNA library established with fresh bovine RPE, expressed this isoform in eukaryotic cells and characterized some of its properties.
authors.affiliation: Institute of Pharmacy, University of Tübingen, Germany. eric.beitz@uni-tuebingen.de
authors.forename: E
authors.initials: E
authors.lastname: Beitz
authors.forename: H
authors.initials: H
authors.lastname: Völkel
authors.forename: Y
authors.initials: Y
authors.lastname: Guo
authors.forename: J E
authors.initials: JE
authors.lastname: Schultz
mesh_terms: D000262:Adenylyl Cyclases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D019556:COS Cells / Q000201:enzymology; D002417:Cattle; D018076:DNA, Complementary / Q000737:chemistry; D005934:Glucagon / Q000494:pharmacology; D006801:Humans; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007668:Kidney; D051379:Mice; D008969:Molecular Sequence Data; D009638:Norepinephrine / Q000494:pharmacology; D010857:Pigment Epithelium of Eye / Q000187:drug effects / Q000201:enzymology*; D016133:Polymerase Chain Reaction; D015398:Signal Transduction; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D014660:Vasoactive Intestinal Peptide; D005934:Glucagon; D000262:Adenylyl Cyclases; D009638:Norepinephrine