An increase in mucosal insulin-like growth factor II content in postresectional rat intestine suggests autocrine or paracrine growth stimulation.
BACKGROUND
Luminal nutrients and growth factors regulate postresectional intestinal growth. The interplay between glutamine and regulatory gastrointestinal peptides is not known.


METHODS
The effects of intestinal resection on tissue and plasma concentrations of peptides were studied in 60 Sprague-Dawley rats divided into resected, transected, or unoperated groups. Subgroups were fed either a glutamine-free or a glutamine-supplemented diet for 7 days. Epidermal growth factor, transforming growth factor-alpha, insulin-like growth factors (IGF) I and II, peptide YY (PYY), and enteroglucagon were analyzed in intestinal mucosa and in portal plasma by radioimmunoassay.


RESULTS
No glutamine-specific effects were seen. The mucosal content of IGF-II (P < 0.01) and the portal levels of enteroglucagon and PYY (P < 0.05-0.01) increased after intestinal resection.


CONCLUSIONS
The increase in PYY and enteroglucagon in portal blood supports a hormonal role in the postresectional adaptation. The increase in IGF II in the ileal mucosa, without changes in plasma, implies autocrine or paracrine growth stimulation at this stage after resection.
Wirén
M
M
Dept. of Surgery, Faculty of Health Sciences, University of Linköping, Sweden.
Adrian
T E
TE
Arnelo
U
U
Permert
J
J
Staab
P
P
Larsson
J
J
D000222:Adaptation, Physiological; D000818:Animals; D004763:Glucagon-Like Peptides / Q000378:metabolism; D005973:Glutamine / Q000008:administration & dosage / Q000494:pharmacology*; D006133:Growth Substances / Q000378:metabolism; D007082:Ileum / Q000378:metabolism / Q000601:surgery; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism / Q000601:surgery; D008297:Male; D019894:Peptide YY / Q000378:metabolism; D011168:Portal System; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D006133:Growth Substances; D005973:Glutamine; D019894:Peptide YY; D004763:Glucagon-Like Peptides; D007335:Insulin-Like Growth Factor II