Comparative distribution of beta-carotene and lycopene after intraperitoneal administration in mice.
To determine the kinetics of accumulation of beta-carotene and lycopene, and their main storage sites, they were separately administered in OFI mice at a single dose of 10 mg/kg body weight or in combination. Animals were sacrificed at given time intervals after intraperitoneal administration and carotenoids were dosed in serum, liver, spleen, kidneys and lungs. A single injection of beta-carotene led to a serum peak at t = 2 h and high levels were detected in the liver after 0.75 hours and in the spleen after 5 hours, with peak values of 10.46 +/- 0.62 and 134 +/- 6 micrograms/g tissue respectively. In contrast, lungs and kidneys did not appear as main accumulation sites. After administration of the carotenoid association, beta-carotene distribution in the four organs was strongly inhibited by lycopene. Concerning lycopene distribution, the concentration values were lower than beta-carotene, the spleen and liver remaining the main storage sites. After administration of a combined dose carotene/lycopene, the percentage of lycopene distribution inhibition was lower compared to the beta-carotene distribution inhibition induced by lycopene. This unusual and non-physiological way of administration for micronutrients leads to high levels of beta-carotene and lycopene in the liver and spleen, and seems of interest in the experimental study and understanding of the biomolecular mechanisms of their activities when administered alone or together.
Glise
D
D
Laboratoire de Biochimie Pharmacie, Faculté de Pharmacie, La Tronche, France.
Riondel
J
J
Favier
A
A
D000818:Animals; D000975:Antioxidants / Q000493:pharmacokinetics*; D001682:Biological Availability; D002338:Carotenoids / Q000493:pharmacokinetics*; D004338:Drug Combinations; D004347:Drug Interactions; D005260:Female; D007274:Injections, Intraperitoneal; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D000077276:Lycopene; D051379:Mice; D013154:Spleen / Q000378:metabolism*; D014018:Tissue Distribution; D019207:beta Carotene / Q000493:pharmacokinetics*
D000975:Antioxidants; D004338:Drug Combinations; D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene