The product of the yvoC (gerF) gene of Bacillus subtilis is required for spore germination.
All known gerF mutations affecting Bacillus subtilis spore germination have been mapped, by a combination of recombination and complementation analysis, to yvoC (Igt), a gene belonging to the yvoB (ptsK) yvoC (Igt) yvoDEF operon. Examination of the properties of null mutants confirmed that the only gene in the operon that affects germination is yvoC, which encodes a homologue of known prelipoprotein diacylglyceryl transferases. As several germination proteins (GerAC, GerBC, GerKC, GerD) are predicted lipoproteins, it is not unreasonable to assume that a defect in prelipoprotein processing will affect spore germination. Two other null mutants in this chromosomal region showed a clear phenotype: the nagA gene is required for growth on N-acetylglucosamine, whereas a null mutation in yvoB (ptsK) affects colony formation from single cells.
Robinson
Carl
C
Department of Molecular Biology and Biotechnology, University of Sheffield,Sheffield 510 ZTN,UK.
Rivolta
Carlo
C
lnstitut de GCnCtique et de Biologie Microbiennes, UniversitC de Lausanne, Rue CCsar-Roux 19, CH-1005 Lausanne,Switzerland.
Karamata
Dimitri
D
lnstitut de GCnCtique et de Biologie Microbiennes, UniversitC de Lausanne, Rue CCsar-Roux 19, CH-1005 Lausanne,Switzerland.
Moir
Anne
A
Department of Molecular Biology and Biotechnology, University of Sheffield,Sheffield 510 ZTN,UK.
D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics* / Q000502:physiology; D016386:Chromosome Walking; D005816:Genetic Complementation Test; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism; D016254:Mutagenesis, Insertional; D009876:Operon / Q000235:genetics / Q000502:physiology; D020161:Physical Chromosome Mapping; D013171:Spores, Bacterial / Q000235:genetics / Q000502:physiology*; D014166:Transferases / Q000235:genetics / Q000378:metabolism*; D014169:Transformation, Bacterial
D008074:Lipoproteins; D014166:Transferases; C042308:phosphatidylglycerol glyceryl transferase