title: Description and demonstration of a CMOS amplifier-based-system with measurement and stimulation capability for bioelectrical signal transduction.
abstract: An extracellular recording system incorporating an electrode array and an amplifier/stimulator CMOS chip is described and characterized. Important features of this custom VLSI chip include 16 instrumentation amplifiers with a gain of 50 and the incorporation of a cross-point array allowing designation of an extracellular microelectrode as either a stimulator or sensor. The planar array consisted of 32 microelectrodes, 14 microns in diameter, and four larger reference electrodes. Microelectrodes, interconnecting traces, and bond pads were patterned with a 500-nm layer of gold. The interconnecting traces were passivated with a 1-micron thick layer of silicon nitride to provide chemical and electrical insulation and microelectrode impedance was lowered utilizing electrode position of platinum black. The amplifier exhibited a nearly flat frequency response with high pass and low pass corner frequencies of 0.7 Hz and 50 kHz, respectively. The input referred noise over the 50 kHz bandwidth was 12-16 microVRMS, well below the magnitude of previously reported extracellular potentials. Crosstalk between neighboring channels resulted in an output signal below the amplifier noise level, even for relatively large extracellular potentials. Using this system, extracellular recording were demonstrated yielding typical peak-to-peak biopotentials of magnitude 0.9-2.1 mV and 100-400 microV for chick cardiac myocytes and rat spinal cord neurons, respectively. The key components of this extracellular recording system can be manufactured using industry standard thin film photolithographic techniques.
authors.affiliation: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Pancrazio
authors.forename: P P
authors.initials: PP
authors.lastname: Bey
authors.forename: A
authors.initials: A
authors.lastname: Loloee
authors.forename: S
authors.initials: S
authors.lastname: Manne
authors.forename: H C
authors.initials: HC
authors.lastname: Chao
authors.forename: L L
authors.initials: LL
authors.lastname: Howard
authors.forename: W M
authors.initials: WM
authors.lastname: Gosney
authors.forename: D A
authors.initials: DA
authors.lastname: Borkholder
authors.forename: G T
authors.initials: GT
authors.lastname: Kovacs
authors.forename: P
authors.initials: P
authors.lastname: Manos
authors.forename: D S
authors.initials: DS
authors.lastname: Cuttino
authors.forename: D A
authors.initials: DA
authors.lastname: Stenger
mesh_terms: D000669:Amplifiers, Electronic; D000818:Animals; D002642:Chick Embryo; D004582:Electronics, Medical / Q000295:instrumentation*; D004594:Electrophysiology; D005069:Evaluation Studies as Topic; D008564:Membrane Potentials; D008839:Microelectrodes; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism