A 1,3-beta-glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is a homologue of the yeast Bgl2p.
A 1,3-beta-glucanosyltransferase which introduces intrachain 1,6-beta linkages into 1,3-beta-glucan was isolated from the cell wall of Aspergillus fumigatus. The biochemical and molecular characterization of the A. fumigatus transferase showed it was homologous to the Saccharomyces cerevisiae and Candida albicans transferase Bgl2p. A null mutant constructed in A. fumigatus by gene replacement did not show a distinct phenotype from the parental strain. The putative function of this major cell-wall-associated protein is discussed.
Okada
Nobuhiko
N
Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
Tatsuno
Lchiro
L
Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
Hanski
Emanuel
E
Department of Clinical Microbiology, The Hebrew University Hadassah Medical School, Jerusalem 91010, Israel.
Caparon
Michael
M
Department of Molecular Microbiology, Washington University School of Medicine, S t Louis, MO 63110, USA.
Sasakawa
Chihiro
C
Department of Bacteriology, Institute of Medical Science, University of Tokyo, Tokyo 1 08, Japan.
D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics; D002473:Cell Wall / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000032:analysis; D005800:Genes, Fungal; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014170:Transformation, Genetic
D004271:DNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; C117939:1,3-beta-glucanosyltransferase; C080389:BGL2 protein, S cerevisiae; D004693:Glucan Endo-1,3-beta-D-Glucosidase