title: Hepatitis E virus: complete genome sequence and phylogenetic analysis of a Nepali isolate.
abstract: The complete nucleotide sequence of the Nepali strain TK15/92 of hepatitis E (HEV) was determined. It showed the highest sequence homology with the Burmese B1 strain, but closer evolutionary relatedness to the Indian strains. Difficulties in reverse-transcribing and amplifying the hypervariable region in ORF1 suggested that strong secondary structures might be intrinsically responsible for the high mutational rate observed in this region of the HEV genome.
authors.affiliation: Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA. imviver@microbio.ufrj.br
authors.forename: V
authors.initials: V
authors.lastname: Gouvea
authors.forename: N
authors.initials: N
authors.lastname: Snellings
authors.forename: M J
authors.initials: MJ
authors.lastname: Popek
authors.forename: C F
authors.initials: CF
authors.lastname: Longer
authors.forename: B L
authors.initials: BL
authors.lastname: Innis
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral; D016679:Genome, Viral; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny
chemical_list: D004279:DNA, Viral