title: Antimicrobial effect of irrigant combinations within dentinal tubules.
abstract: The aim of this study was to investigate sodium hypochlorite (with and without EDTA), chlorhexidine, and hydrogen peroxide in varying concentrations when used in sequence or in combination as endodontic irrigants. Sterile saline served as the control. Six standardized bovine incisor root specimens, which had been infected with Enterococcus faecalis (CG 110), were exposed to each solution. Following exposure to the test agents dentine samples were taken from within the lumina using ISO 023 to 035 burs. The dentine powder samples were incubated and the quantity of bacteria present assessed using spectrophotometry. All irrigant regimens were more effective than saline in killing bacteria. Chlorhexidine and sodium hypochlorite were similarly effective. At specific concentrations a synergistic effect was noted when mixtures of chlorhexidine and hydrogen peroxide were under test.
authors.affiliation: Department of Endodontics, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Heling
authors.forename: N P
authors.initials: NP
authors.lastname: Chandler
mesh_terms: D000818:Animals; D002417:Cattle; D002710:Chlorhexidine / Q000494:pharmacology; D003804:Dentin / Q000382:microbiology*; D004338:Drug Combinations; D004492:Edetic Acid / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects; D005069:Evaluation Studies as Topic; D006861:Hydrogen Peroxide / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology
chemical_list: D004338:Drug Combinations; D012388:Root Canal Irrigants; D004492:Edetic Acid; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D002710:Chlorhexidine