title: Liver glutathione content and glutathione-dependent enzymes of two species of freshwater fish as bioindicators of chemical pollution.
abstract: Glutathione content and glutathione-dependent enzymes were measured in the liver of two fish species, gudgeon (Gobio gobio) and roach (Rutilus arcasii), from the river Bernesga (Spain) caught downstream and upstream of the waste site of several chemical industries. Animals from contaminated sites display a reduced glutathione concentration and a tendency to the decrease of glutathione S-transferase activity. Glutathione peroxidase activity was significantly elevated only in the liver of Gobio gobio and glutathione reductase activity in that of Rutilus arcasii. Our data indicate that the glutathione system constitutes a sensitive biochemical indicator of chemical pollution. Relative changes of glutathione and glutathione-dependent enzymes in both fish species suggest a different susceptibility to toxins.
authors.affiliation: Department of Physiology, Pharmacology and Toxicology, University of León, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Almar
authors.forename: L
authors.initials: L
authors.lastname: Otero
authors.forename: C
authors.initials: C
authors.lastname: Santos
authors.forename: J
authors.initials: J
authors.lastname: González Gallego
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000379:methods; D005399:Fishes / Q000378:metabolism*; D005618:Fresh Water / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis*; D005982:Glutathione Transferase / Q000032:analysis*; D007220:Industrial Waste; D013030:Spain; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D017392:Thiobarbituric Acid Reactive Substances; D014874:Water Pollutants, Chemical; D005982:Glutathione Transferase; D005978:Glutathione