title: The use of nested RT-PCR of prostate-specific membrane antigen in blood cells: implications for the detection of haematogenous neoplastic cells in patients with prostate adenocarcinoma.
abstract: The aim of this study was to determine the presence of haematogenous neoplastic cells in patients with prostate cancer. Circulating prostate cells can be detected in cancer patients by using a nested-reverse transcriptase-polymerase chain reaction assay (RT-PCR), for prostate-specific membrane (PSM) antigen mRNA. This sensitive nested RT-PCR assay may play a crucial role in the administration of adjuvant therapy of patients with prostate adenocarcinoma.
authors.affiliation: Regional Center of Neurogenetics, Service of Neurology, Maison Blanche Hospital, 45 rue Cognacq-Jay, Reims, France.
authors.forename: G
authors.initials: G
authors.lastname: Lucotte
authors.forename: G
authors.initials: G
authors.lastname: Mercier
authors.forename: A
authors.initials: A
authors.lastname: Burckel
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000235:genetics / Q000473:pathology; D000368:Aged; D000369:Aged, 80 and over; D000951:Antigens, Neoplasm / Q000097:blood / Q000235:genetics*; D000954:Antigens, Surface / Q000097:blood / Q000235:genetics*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D017931:DNA Primers; D004273:DNA, Neoplasm; D012333:RNA, Messenger