title: Structure of a human DNA repair protein UBA domain that interacts with HIV-1 Vpr.
abstract: The HIV-1 protein Vpr is critical for a number of viral functions including a unique ability to arrest T-cells at a G2/M checkpoint and induce subsequent apoptosis. It has been shown to interact specifically with the second UBA (ubiquitin associated) domain found in the DNA repair protein HHR23A, a highly evolutionarily conserved protein. This domain is a commonly occurring sequence motif in some members of the ubiquitination pathway, UV excision repair proteins, and certain protein kinases. The three dimensional structure of the UBA domain, determined by NMR spectroscopy, is presented. The protein domain forms a compact three-helix bundle. One side of the protein has a hydrophobic surface that is the most likely Vpr target site.
authors.affiliation: Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California at Los Angeles, 90095, USA.
authors.forename: T
authors.initials: T
authors.lastname: Dieckmann
authors.forename: E S
authors.initials: ES
authors.lastname: Withers-Ward
authors.forename: M A
authors.initials: MA
authors.lastname: Jarosinski
authors.forename: C F
authors.initials: CF
authors.lastname: Liu
authors.forename: I S
authors.initials: IS
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Feigon
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D004260:DNA Repair; D045643:DNA Repair Enzymes; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D016336:Gene Products, vpr / Q000378:metabolism*; D015497:HIV-1; D006367:HeLa Cells; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010449:Peptide Mapping; D011487:Protein Conformation; D014452:Ubiquitins / Q000378:metabolism; D054325:vpr Gene Products, Human Immunodeficiency Virus
chemical_list: D004268:DNA-Binding Proteins; D016336:Gene Products, vpr; D014452:Ubiquitins; D054325:vpr Gene Products, Human Immunodeficiency Virus; C086985:RAD23A protein, human; D045643:DNA Repair Enzymes