Day and night dysfunction in intraretinal melatonin and related indoleamines metabolism, correlated with the development of glaucoma-like disorder in an avian model.
As previous studies have suggested that melatonin and serotonin may be involved in the regulation of intraocular pressure, retinal concentrations of melatonin, 5-HT, and related indoleamines measured at day and at night were studied during the development of a glaucoma-like disorder with increased intraocular pressure in the al mutant quail. Indoleamine levels were determined by HPLC with electrochemical detection in 1-month-, 3-month-, and 7-month-old al mutant and control quails. Morphology and numbers of melatonin-synthesizing and 5-HT-containing cells, labelled immunohistochemically with an anti-hydroxyindol-0-methyltransferase (HIOMT) antibody and an anti-5-HT antibody, respectively, were studied. Major findings were that: (1) no significant changes in morphology of melatonin-synthesizing cells or in the morphology and density of 5-HT-containing amacrine cells were observed during the development of glaucoma: (2) 5-HT metabolism was modified during the night at 1 month of age and during the day after 3 months; and (3) melatonin metabolism was modified during the night at 7 months and during the day after 3 months. These results demonstrate a relationship between the temporal evolution of this avian glaucoma and a dysfunction in indoleamine retinal metabolism.
Dkhissi
O
O
Laboratoire de NeuroCytologie Oculaire, INSERM U 86, Paris, France.
Chanut
E
E
Versaux-Botteri
C
C
Trouvin
J H
JH
Repérant
J
J
Nguyen-Legros
J
J
D000121:Acetylserotonin O-Methyltransferase / Q000378:metabolism; D000375:Aging / Q000378:metabolism; D000588:Amines / Q000378:metabolism*; D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D003370:Coturnix / Q000235:genetics; D005901:Glaucoma / Q000209:etiology*; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D007211:Indoles / Q000378:metabolism*; D008550:Melatonin / Q000378:metabolism*; D009154:Mutation / Q000502:physiology; D012016:Reference Values; D012160:Retina / Q000378:metabolism*; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism
D000588:Amines; D007211:Indoles; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D000121:Acetylserotonin O-Methyltransferase; D008550:Melatonin; C006389:N-acetylserotonin