Frequency of trisomy 20 in nonmalignant bronchial epithelium from lung cancer patients and cancer-free former uranium miners and smokers.
Lung cancer is the leading cause of cancer-related deaths. The development of sensitive screening methods to identify at-risk individuals before emergence of clinical disease would permit early intervention that could decrease this mortality. Our previous studies have shown that cells with trisomy 7 can be detected in bronchial epithelium from cancer-free smokers and former uranium miners. However, the use of more than one molecular marker could increase the chance of identifying at-risk individuals. Trisomy 20, which is found in 43-57% of non-small cell lung cancers, is a candidate marker. The purpose of the current investigation was to determine the percentage of cells with trisomy 20 in persons with a high risk for lung cancer. Bronchial epithelial cells that had been assayed for trisomy 7 were assayed for trisomy 20 by fluorescence in situ hybridization. Trisomy 20 was detected in bronchial epithelial cells from lung cancer patients and from smokers and ex-uranium miners without lung cancer. In some cases, patients who were negative for trisomy 7 exhibited trisomy 20. Consequently, more people with field cancerization were identified using both markers. However, the two markers combined did not appear to stratify the risk for lung cancer.
Neft
R E
RE
Lovelace Respiratory Research Institute, Albuquerque, New Mexico 87185, USA. RNEFT@lrri.org
Crowell
R E
RE
Gilliland
F D
FD
Murphy
M M
MM
Lane
J L
JL
Harms
H
H
Coons
T
T
Heaphy
E
E
Belinsky
S A
SA
Lechner
J F
JF
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor; D001980:Bronchi / Q000166:cytology*; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000517:prevention & control*; D002478:Cells, Cultured; D002890:Chromosomes, Human, Pair 20 / Q000235:genetics*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008175:Lung Neoplasms / Q000235:genetics* / Q000517:prevention & control*; D008297:Male; D008875:Middle Aged; D008906:Mining; D016273:Occupational Exposure / Q000009:adverse effects*; D011237:Predictive Value of Tests; D012907:Smoking / Q000009:adverse effects*; D014314:Trisomy; D014501:Uranium / Q000009:adverse effects*
D014408:Biomarkers, Tumor; D014501:Uranium