title: Further complexity of the human SOX gene family revealed by the combined use of highly degenerate primers and nested PCR.
abstract: SOX proteins contain a conserved HMG-related DNA-binding domain. They fulfill essential functions during the development of animals. Mutations in several SOX genes have been implicated in human diseases. We present here a new set of PCR primers designed to amplify a broad range of SOX HMG-box sequences. These primers facilitated the cloning of several new SOX HMG boxes from human genomic DNA, revealing unexpected complexity of the SOX gene family.
authors.affiliation: Human Molecular Genetic Group, Institut de Génétique Humaine, UPR CNRS 1142, Montpellier, France.
authors.forename: F
authors.initials: F
authors.lastname: Cremazy
authors.forename: S
authors.initials: S
authors.lastname: Soullier
authors.forename: P
authors.initials: P
authors.lastname: Berta
authors.forename: P
authors.initials: P
authors.lastname: Jay
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D017124:Conserved Sequence; D017931:DNA Primers; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D006609:High Mobility Group Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008322:Mammals; D051379:Mice; D008969:Molecular Sequence Data; D005810:Multigene Family; D009687:Nuclear Proteins; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D009687:Nuclear Proteins; D011994:Recombinant Proteins; D051876:Sex-Determining Region Y Protein; D014157:Transcription Factors