Determination of trans-beta-carotene and other carotenoids in blood plasma using high-performance liquid chromatography and thermal lens detection.
Thermal lens spectrometry (TLS) was applied for the detection of beta-cryptoxanthin, alpha-carotene, trans-beta-carotene, and lycopen in blood plasma. This combined high-performance liquid chromatography-TLS (HPLC-TLS) method was validated by comparison with HPLC-UV-Vis analysis of blood plasma under identical chromatographic conditions and by comparing the results obtained from an independent, standard HPLC procedure for determination of carotenoids in blood plasma samples. The results demonstrated good agreement with the target values for carotenoids in an in-laboratory control sample and confirmed the accuracy of the HPLC-TLS technique. Limits of detection for blood plasma samples were 70 pg/ml for beta-cryptoxanthin, 85 pg/ml for alpha-carotene, 100 pg/ml for trans-beta-carotene, and 120 pg/ml for lycopen. This represents a 100-fold improvement compared to the HPLC analysis with UV-Vis detection.
Franko
M
M
School of Environmental Sciences, Nova Gorica, Slovenia.
van de Bovenkamp
P
P
Bicanic
D
D
D002338:Carotenoids / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D000077276:Lycopene; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*; D019207:beta Carotene / Q000097:blood*
D019207:beta Carotene; D002338:Carotenoids; D000077276:Lycopene