title: Separation and quantification by ion-association capillary zone electrophoresis of unsaturated disaccharide units of chondroitin sulfates and oligosaccharides derived from hyaluronan.
abstract: An analytical method has been developed for assay of unsaturated disaccharides of chondroitin sulfates and of oligosaccharides (tetra- and hexasaccharides) of hyaluronan, using ion-association capillary zone electrophoresis. Samples were applied at the anode (the usual polarity), using a borate buffer modified by an ion-pairing reagent, tetrabutylammonium (TBA) phosphate, and the effect of the concentration of the ion-pairing reagent on various electrophoretic parameters (electroosmotic flow, electrophoretic mobility of products, capacity factors) was observed. Increasing concentrations of the reagent led to a decrease of zeta potential, probably due to specific adsorption of the quaternary ammonium ion onto the capillary wall. The authors propose a mechanism of separation, in which anionic borate complexes are formed and interact with TBA ion inside the capillary tube. The capillary electrophoretic system described is potentially a powerful method for specific measurement of the concentrations in joint tissues of chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronan, whose relative abundances may vary in various diseases or after local treatments with, for example, antiinflammatory drugs, chondroprotective agents, or orthopedic implants.
authors.affiliation: Laboratoire de Pharmacologie, CNRS-UHP, Faculté de Médecine, Nancy, France.
authors.forename: E
authors.initials: E
authors.lastname: Payan
authors.forename: N
authors.initials: N
authors.lastname: Presle
authors.forename: F
authors.initials: F
authors.lastname: Lapicque
authors.forename: J Y
authors.initials: JY
authors.lastname: Jouzeau
authors.forename: K
authors.initials: K
authors.lastname: Bordji
authors.forename: S
authors.initials: S
authors.lastname: Oerther
authors.forename: G
authors.initials: G
authors.lastname: Miralles
authors.forename: D
authors.initials: D
authors.lastname: Mainard
authors.forename: P
authors.initials: P
authors.lastname: Netter
mesh_terms: D002809:Chondroitin Sulfates / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis*; D019075:Electrophoresis, Capillary; D006820:Hyaluronic Acid / Q000032:analysis*; D007202:Indicators and Reagents; D009844:Oligosaccharides / Q000032:analysis*
chemical_list: D004187:Disaccharides; D007202:Indicators and Reagents; D009844:Oligosaccharides; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates