Simultaneous degradation of chloro- and methyl-substituted aromatic compounds: competition between Pseudomonas strains using the ortho and meta pathway or the ortho pathway exclusively.
Pseudomonas sp. D7-4 and Pseudomonas sp. B13 FR1(pFRC20P) degraded mixtures of chloro- and methyl-substituted benzoates exclusively via an extended ortho pathway, whereas in Pseudomonas putida WR 201 both ortho and meta fission were induced by mixtures of 3-chloro- and 3-methylbenzoate or even by 3-chloro-benzoate alone. The competition behaviour of these strains was compared in batch and in chemostat cultures.. Despite misrouting of metabolites, strain WR201 was competitive, in a lot of the competition experiments, with mixtures of these substrates. Only in a narrow range of the mixing ratio of chloro- and methylbenzoate was the presence of both the meta and ortho pathways a disadvantage for competitiveness. Outside these ranges other attributes, such as high growth rates or short lag periods, or a respective strain were even more essential for one strain to outcompete another.
Franck-Mokross
A C
AC
Bergische Universität-Gesamthochschule Wuppertal, Chemische Mikrobiologie, Wuppertal, Germany.
Schmidt
E
E
D001565:Benzoates / Q000378:metabolism*; D001673:Biodegradation, Environmental; D050557:Catechol 2,3-Dioxygenase; D002723:Chlorobenzoates / Q000378:metabolism*; D003470:Culture Media; D049308:Dioxygenases; D008745:Methylation; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*
D001565:Benzoates; D002723:Chlorobenzoates; D003470:Culture Media; C036427:3-chlorobenzoic acid; C009504:4-toluic acid; D010105:Oxygenases; D049308:Dioxygenases; D050557:Catechol 2,3-Dioxygenase