Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix.
Negative-ion matrix-assisted laser desorption/ionization mass spectra of sialyated glycoconjugates were acquired employing 2,5-dihydroxybenzoic acid (DHB) in conjunction with spermine as a co-matrix. The addition of spermine to DHB permitted an improved crystal formation as well as a higher analyte solubility. Moreover, DHB/spermine appears to minimize alkali adduct formation, thus allowing the sample analysis without desalting. The combined matrix permitted the analysis of complex sialylated and sialylated/fucosylated structures down to the femotomole range. The ability to use such a matrix also facilitates determination of the sialic acid linkages (in combination with a specific enzyme cleavage). The matrix also appears suitable for studies on gangliosides.
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Department of Chemistry, Indiana University, Bloomington 47401, USA.
Novotny
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MV
D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D003460:Crystallization; D005841:Gentisates; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D006868:Hydrolysis; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D008320:Maltose / Q000737:chemistry; D008969:Molecular Sequence Data; D012794:Sialic Acids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013096:Spermine / Q000737:chemistry*
D005841:Gentisates; D006023:Glycoproteins; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D012794:Sialic Acids; D013096:Spermine; D008320:Maltose; C010925:2,5-dihydroxybenzoic acid