Platelet-like particle production from cultured human megakaryocytic cell line.
The human megakaryocytic cell line CMK was positive for the expression of platelet glycoprotein(GP) IIb/IIIa but few cells were positive for GPIb. To establish a GPIb-positive CMK subclone, GPIb-positive CMK cells were purified, micromanipulated and cloned, but resulting sublines were all negative for GPIb expression. The combined presence of interleukin-3 (IL-3) and the anti-virus agent ribavirin strongly induced expression of GPIb on the cells. However, the megakaryocyte growth and development factor (MGDF)/thrombopoietin (TPO) failed to enhance GPIb expression. CMK themselves and particles are negative for GPIb expression. To utilize these particles as platelet substitutes, further appropriate culture systems are necessary.
Ikebuchi
K
K
Hokkaido Red Cross Blood Center, Japan.
Yamaguchi
M
M
Niwa
K
K
Abe
H
H
Fujihara
M
M
Sekiguchi
S
S
D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D001802:Blood Substitutes / Q000378:metabolism*; D018929:Cell Culture Techniques; D002460:Cell Line; D006801:Humans; D016130:Immunophenotyping; D008533:Megakaryocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*
D001802:Blood Substitutes