title: Engineering chromosomes in mice through targeted meiotic recombination (TAMERE).
abstract: Functional studies of large transcription units, clustered genes and chromosomal loci require the design of novel experimental tools to engineer genomic macro-rearrangements. Here, we present a strategy to produce deficiencies or duplications by crossing mice carrying loxP sites in homologous loci. This trans-allelic targeted meiotic recombination (TAMERE) protocol allows for the combination of various alleles within a particular locus as well as for generation of interchromosomal unequal exchanges. Novel genetic configurations can thus be produced without multiple targeting and selection steps in embryonic stem (ES) cells. A concomitant deletion/duplication event of the Hoxdl2 locus shows the potential of this approach. The high frequency of such targeted exchanges in vivo makes TAMERE a powerful genetic tool applicable to research areas in which complex genomic modifications are required.
authors.affiliation: Department of Zoology and Animal Biology, University of Geneva, Science III, Switzerland.
authors.forename: Y
authors.initials: Y
authors.lastname: Hérault
authors.forename: M
authors.initials: M
authors.lastname: Rassoulzadegan
authors.forename: F
authors.initials: F
authors.lastname: Cuzin
authors.forename: D
authors.initials: D
authors.lastname: Duboule
mesh_terms: D000818:Animals; D001483:Base Sequence; D002875:Chromosomes; D003433:Crosses, Genetic; D004247:DNA; D005260:Female; D017353:Gene Deletion; D020440:Gene Duplication; D005801:Genes, Homeobox; D005818:Genetic Engineering; D008297:Male; D008540:Meiosis / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D011995:Recombination, Genetic
chemical_list: D004247:DNA