title: Preadministration of incadronate disodium can prevent bone loss in rat proximal tibial metaphysis when induced by hindlimb immobilization by bandage.
abstract: The purpose of this study is to determine whether short-term preadministration of bisphosphonates prevents bone loss in rat proximal tibial metaphysis when induced by hindlimb immobilization by bandage. Six-month-old female Sprague Dawley rats were injected with incadronate disodium (YM-175, 10 micrograms/kg) or vehicle, three times per week for 2 weeks (YM or V groups). Then, the left hindlimb was fixed to the abdomen with a bandage (V-B, YM-B groups), or only the abdomen was bandaged as control (V-SHM, YM-SHM groups), for 4 weeks. The animals were subsequently killed and left proximal tibiae were processed undecalcified for quantitative histomorphometric evaluation. Immobilization-induced cancellous bone loss resulted not only from increased percent eroded surface area but also from decreased percent labeling surface and bone formation rate in V-B compared with V-SHM animals. In contrast, preadministration of YM-175 decreased percent eroded surface significantly and prevented the loss of cancellous bone mass in YM-B compared with V-B animals. Cancellous bone mass was neither increased nor decreased by preadministration of YM-175 in YM-SHM animals. Our results suggest that preadministration of bisphosphonates is effective in prevention of bone loss at the tibial metaphysis when induced by hindlimb immobilization in rats.
authors.affiliation: Department of Orthopedic Surgery, Kagawa Medical University, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: T
authors.initials: T
authors.lastname: Mashiba
authors.forename: Y
authors.initials: Y
authors.lastname: Kaji
authors.forename: M
authors.initials: M
authors.lastname: Taki
authors.forename: S
authors.initials: S
authors.lastname: Komatsubara
authors.forename: J
authors.initials: J
authors.lastname: Kawanishi
authors.forename: H
authors.initials: H
authors.lastname: Norimatsu
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001851:Bone Diseases, Metabolic / Q000503:physiopathology / Q000517:prevention & control*; D004164:Diphosphonates / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D006614:Hindlimb / Q000503:physiopathology; D007091:Image Processing, Computer-Assisted; D007103:Immobilization / Q000009:adverse effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013977:Tibia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology
chemical_list: D004164:Diphosphonates; C071542:cimadronate