On-line sample preparation of paraquat in human serum samples using high-performance liquid chromatography with column switching.
A new ion-pair high-performance liquid chromatographic method with column-switching has been developed for the determination of paraquat in human serum samples. The diluted serum sample was injected onto a precolumn packed with LiChroprep RP-8 (25-40 microm) and polar serum components were washed out by 3% acetonitrile in 0.05 M phosphate buffer (pH 2.0) containing 5 mM sodium octanesulfonate. After valve switching to inject position, concentrated compounds were eluted in the back-flush mode and separated on an Inertsil ODS-2 column with 17% acetonitrile in 0.05 M phosphate buffer (pH 2.0) containing 10 mM sodium octanesulfonate. The total analysis time per sample was about 30 min and mean recovery was 98.5+/-2.8% with a linear range of 0.1-100 microg/ml. This method has been successfully applied to serum samples from incidents by paraquat poisoning.
Lee
H S
HS
College of Pharmacy, Wonkwang University, Iksan, South Korea.
Kim
K
K
Kim
J H
JH
Do
K S
KS
Lee
S K
SK
D000097:Acetonitriles; D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D010269:Paraquat / Q000097:blood* / Q000506:poisoning; D010710:Phosphates; D015203:Reproducibility of Results
D000097:Acetonitriles; D002021:Buffers; D010710:Phosphates; D010269:Paraquat; C032159:acetonitrile