Carnoy's fixative reduces the number of chymase-positive cells in immunocytochemical staining of cord-blood-derived human cultured mast cells.
KleinJan et al. (Allergy 1996;51:614-20) reported that Carnoy's fixative reduced the number of chymase-positive mast cells in the nasal mucosa. Therefore, in the present study, we investigated whether Carnoy's fixative reduces the number of chymase-positive cells from cord-blood-derived human cultured mast cells when compared with other types of fixatives. Human mast cells were obtained by culturing cord-blood-derived CD34-positive cells in the presence of stem cell factor and interleukin-6. Staining procedures of the cells in fixation with Carnoy's fixative and with other fixatives gave no differences among the number of tryptase-positive cells, whereas fixation with Carnoy's fixative for 15 min gave a significant decrease in the number of chymase-positive cells compared with acetone for 10 min. The number of chymase-positive cells decreased in a time-dependent manner under fixation with Carnoy's fixative, indicating that Carnoy's fixative had a negative effect on the number of chymase-positive cells from cord-blood-derived human cultured mast cells.
Kanbe
N
N
Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
Kurosawa
M
M
Miyachi
Y
Y
Kanbe
M
M
Kempuraj
D
D
Tachimoto
H
H
Saito
H
H
D019342:Acetic Acid / Q000494:pharmacology*; D000096:Acetone / Q000494:pharmacology; D002452:Cell Count / Q000187:drug effects; D002478:Cells, Cultured; D002725:Chloroform / Q000494:pharmacology*; D053818:Chymases; D000431:Ethanol / Q000494:pharmacology*; D005312:Fetal Blood / Q000166:cytology*; D005404:Fixatives / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000494:pharmacology; D008407:Mast Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology; D012697:Serine Endopeptidases / Q000032:analysis*; D012997:Solvents / Q000494:pharmacology; D013194:Staining and Labeling; D019089:Stem Cell Factor / Q000494:pharmacology; D013997:Time Factors; D016707:Tissue Fixation / Q000379:methods
C033474:Carnoy's solution; D005404:Fixatives; D015850:Interleukin-6; D012997:Solvents; D019089:Stem Cell Factor; D000096:Acetone; D000431:Ethanol; D002725:Chloroform; D012697:Serine Endopeptidases; D053818:Chymases; D019342:Acetic Acid