title: Generation, identification, and evaluation of expressed sequence tags from different developmental stages of the Asian blood fluke Schistosoma japonicum.
abstract: Here we report 658 expressed sequence tags (ESTs) generated from the 5'-termini of clones randomly selected from directional cDNA libraries constructed from mRNAs from three developmental stages of Schistosoma japonicum. Putative identifications were assigned to 46. 2% of the ESTs; 6.4% were previously known from S. japonicum, 5.6% were previously known from S. mansoni, 34.2% were known from other organisms, and the remaining 53.8% may represent S. japonicum-specific genes. These 658 ESTs appeared to be derived from 457 unique genes, which together represent 2 to 3% of the 15,000 to 20,000 genes predicted to occur in the schistosome genome.
authors.affiliation: Molecular Parasitology Unit and Australian Centre for International & Tropical Health & Nutrition, The Queensland Institute of Medical Research, Brisbane, Queensland, Australia.
authors.forename: J
authors.initials: J
authors.lastname: Fan
authors.forename: D J
authors.initials: DJ
authors.lastname: Minchella
authors.forename: S R
authors.initials: SR
authors.lastname: Day
authors.forename: D P
authors.initials: DP
authors.lastname: McManus
authors.forename: W U
authors.initials: WU
authors.lastname: Tiu
authors.forename: P J
authors.initials: PJ
authors.lastname: Brindley
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018509:DNA, Helminth / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D017238:Genes, Helminth; D008297:Male; D051379:Mice; D018510:RNA, Helminth / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D012549:Schistosoma japonicum / Q000235:genetics* / Q000254:growth & development*
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D018509:DNA, Helminth; D018510:RNA, Helminth; D012333:RNA, Messenger