Cultured sympathetic neurons express functional interleukin-1 receptors.
Since recent work has identified an apoptotic pathway in sympathetic neurons that is mediated by autocrine interleukin-1 (IL-1), we investigated whether cultured sympathetic neurons possess functional IL-1 receptors. Cultured sympathetic neurons express levels of IL-1RI and IL-1RAcP mRNAs consistent with signal transduction. Neurons stimulated with IL-1 demonstrate enhanced p65 NF-kappaB nuclear translocation and enhanced NF-kappaB DNA binding activity, with at least p65 and p50 subunits participating in the DNA binding activity. RNA differential display identified several neuronal mRNAs regulated by IL-1, including a member of the reticulon family. We conclude that IL-1 stimulates a potential component of a neuronal secretory pathway.
Bai
Y
Y
Department of Biological Sciences, Rutgers University, Newark, NJ 07102-1811, USA.
Hart
R P
RP
D000818:Animals; D017209:Apoptosis / Q000276:immunology; D019898:Autocrine Communication / Q000276:immunology; D002478:Cells, Cultured; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000276:immunology; D015870:Gene Expression / Q000276:immunology; D053592:Interleukin-1 Receptor-Associated Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D020929:Mitogen-Activated Protein Kinase Kinases; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D009474:Neurons / Q000166:cytology* / Q000276:immunology*; D011494:Protein Kinases / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology; D017783:Superior Cervical Ganglion / Q000166:cytology* / Q000276:immunology
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D016328:NF-kappa B; D012333:RNA, Messenger; D017472:Receptors, Interleukin-1; D011494:Protein Kinases; D053592:Interleukin-1 Receptor-Associated Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D020929:Mitogen-Activated Protein Kinase Kinases