Serum antibodies to heparan sulfate glycosaminoglycans in Guillain-Barré syndrome and other demyelinating polyneuropathies.
We tested for serum antibodies to glycosaminoglycans (GAGs), including heparan sulfate, in patients with Guillain-Barré syndrome (GBS) and other disorders. We used ELISA methods that optimize immunoglobulin binding to carbohydrate antigens to measure serum antibodies to heparan sulfate GAGs in GBS, and control neuromuscular and immune disorders. We found serum IgM or IgG antibodies to heparan sulfate GAGs in 34% of patients with GBS. Serum IgM binding to heparan sulfate GAGs was also found in some chronic demyelinating polyneuropathies, with the highest frequency (33%) in patients with IgM anti-MAG M-proteins. Antibodies to heparan sulfate GAGs were rare (1%) in control serums from patients with other disorders. This result is the first demonstration of high titer serum antibodies to a specific antigen in a substantial group of, and with some specificity for, patients with the classically described GBS syndrome of acute-onset, motor-sensory polyneuropathy with demyelinating features.
Pestronk
A
A
Department of Neurology, Washington University School of Medicine, St. Louis, MO 63110, USA. pestronk@kids.wustl.edu
Choksi
R
R
Yee
W C
WC
Kornberg
A J
AJ
Lopate
G
G
Trotter
J
J
D001323:Autoantibodies / Q000097:blood*; D003711:Demyelinating Diseases / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006025:Glycosaminoglycans / Q000276:immunology; D019812:Heparan Sulfate Proteoglycans / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011115:Polyneuropathies / Q000276:immunology*; D011129:Polyradiculoneuropathy / Q000276:immunology*
D001323:Autoantibodies; D006025:Glycosaminoglycans; D019812:Heparan Sulfate Proteoglycans; D007074:Immunoglobulin G; D007075:Immunoglobulin M