Activities of enzymes of acetyl-CoA and acetylcholine metabolism in SN56 hybrid cholinergic cell line differentiated by dibutyryl cyclic AMP and all-trans retinoic acid.
Cholinergic hybrid mouse septal neurons SN56 were differentiated by separate and combined application of 0.001 mM all-trans-retinoic acid and 1 mM dibutyryl cAMP. Each of agents caused about twofold increase of choline acetyltransferase activity. These activatory effects were additive. Dibutyryl cAMP resulted in twofold increase of ATP-citrate lyase and acetylcholinesterase activities. Retinoic acid did not affect these enzyme activities but partially abolished activatory effects of dibutyryl cAMP. Pyruvate dehydrogenase and other enzymes of acetyl-CoA metabolism were not affected by this treatment. This work demonstrates that it is possible to rise cholinergic neurons of different expression of cholinergic and acetyl-CoA metabolism.
Jankowska
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A
Department of Clinical Biochemistry, School of Medicine, Gdańsk, Poland.
Blusztajn
J K
JK
Szutowicz
A
A
D001275:ATP Citrate (pro-S)-Lyase / Q000096:biosynthesis* / Q000235:genetics; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000109:Acetylcholine / Q000378:metabolism*; D000110:Acetylcholinesterase / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002795:Choline O-Acetyltransferase / Q000096:biosynthesis* / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D006822:Hybrid Cells / Q000187:drug effects*; D051379:Mice; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009447:Neuroblastoma / Q000473:pathology; D012688:Septum Pellucidum / Q000473:pathology; D014212:Tretinoin / Q000494:pharmacology*
D009419:Nerve Tissue Proteins; D014212:Tretinoin; D003994:Bucladesine; D000105:Acetyl Coenzyme A; D002795:Choline O-Acetyltransferase; D001275:ATP Citrate (pro-S)-Lyase; D000110:Acetylcholinesterase; D000109:Acetylcholine