title: Chromosome 11q13 gene amplifications in oral and oropharyngeal carcinomas: no correlation with subclinical lymph node invasion and disease recurrence.
abstract: Gene amplifications in the q13 band of chromosome 11 are among the most frequent genetic alterations in head and neck squamous cell carcinomas. Previous studies have suggested that such amplification is a marker of aggressive tumor evolution. Their potential for predicting subclinical lymph node invasion or disease recurrence was investigated in a prospective series of 50 oral and oropharyngeal carcinomas. Cell DNA content was also measured in 32 tumors of this series. Gene amplifications affecting the 11q13 band were detected in 11 of 50 (20%) patients, a relatively low frequency in comparison with data reported previously for other carcinomas of the upper aerodigestive tract, especially hypopharyngeal carcinomas. These gene amplifications were preferentially associated with aneuploidy. Cervical lymph nodes of 26 clinically N0 (Tumor-Node-Metastasis staging) patients were surgically explored. The frequency of 11q13 amplifications was very similar in the presence or in the absence of histological invasion, 3 of 15 (20%) and 2 of 11 (18%), respectively. Thus, 11q13 amplifications do not appear to be a reliable marker for prediction of subclinical lymph-node invasion in oral and oropharyngeal carcinomas. The detection of 11q13 amplifications was also not associated with a higher risk of disease recurrence. These data suggest that not only the prevalence but also the prognostic significance of 11q13 amplifications varies between tumors at different sites in the upper aerodigestive tract.
authors.affiliation: Déepartement de Radiothérapie, Laboratoire de Biologie des Tumeurs Humaines, Institut Gustave Roussy, 94805 Villejuif Cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Fortin
authors.forename: M
authors.initials: M
authors.lastname: Guerry
authors.forename: R
authors.initials: R
authors.lastname: Guerry
authors.forename: M
authors.initials: M
authors.lastname: Talbot
authors.forename: O
authors.initials: O
authors.lastname: Parise
authors.forename: G
authors.initials: G
authors.lastname: Schwaab
authors.forename: J
authors.initials: J
authors.lastname: Bosq
authors.forename: J
authors.initials: J
authors.lastname: Bourhis
authors.forename: P
authors.initials: P
authors.lastname: Salvatori
authors.forename: F
authors.initials: F
authors.lastname: Janot
authors.forename: P
authors.initials: P
authors.lastname: Busson
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000782:Aneuploidy; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics*; D019938:Cyclin D1 / Q000032:analysis; D005260:Female; D005434:Flow Cytometry; D005784:Gene Amplification; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis; D009364:Neoplasm Recurrence, Local; D009857:Oncogenes; D010610:Pharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D011379:Prognosis; D011446:Prospective Studies; D012306:Risk
chemical_list: D009363:Neoplasm Proteins; D019938:Cyclin D1