Effects of sample preparation conditions on biomolecular solid-state NMR lineshapes.
Sample preparation conditions with the 46 kDa enzyme complex of 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase, shikimate-3-phosphate (S3P) and glyphosate (GLP) have been examined in an attempt to reduce linewidths in solid-state NMR spectra. The linewidths of 31P resonances associated with enzyme bound S3P and GLP in the lyophilized ternary complex have been reduced to 150 +/- 12 Hz and 125 +/- 7 Hz respectively, by a variety of methods involving additives and freezing techniques.
Jakeman
D L
DL
Department of Biochemistry and Biophysics, Washington State University, Pullman 99163-4660, USA.
Mitchell
D J
DJ
Shuttleworth
W A
WA
Evans
J N
JN
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D005615:Freezing; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D046911:Macromolecular Substances; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011092:Polyethylene Glycols; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D013048:Specimen Handling; D014199:Trehalose; D000097797:Glyphosate
D051229:3-Phosphoshikimate 1-Carboxyvinyltransferase; D019883:Alkyl and Aryl Transferases; D001426:Bacterial Proteins; D005998:Glycine; D046911:Macromolecular Substances; D011092:Polyethylene Glycols; D012765:Shikimic Acid; D014199:Trehalose; D000097797:Glyphosate; C042786:shikimic acid-3-phosphate