title: HTLV-II risk factors in Native Americans in Florida.
abstract: Earlier virologic studies established that human T-cell lymphotropic virus type II (HTLV-II) is the predominant retrovirus type found among Seminole Indians in southern Florida. We studied 46 members of the Seminole tribe living on 3 reservations to determine the risk factors for HTLV-II and to investigate disease association with the virus. The donors' plasma samples were evaluated with the enzyme-linked immunosorbent and Western blot assays. DNA extracted from their peripheral blood mononuclear cells were analyzed by type-specific polymerase chain reaction amplification and detection of the HTLV pol gene using the primer pair SK110/SK111, and the probes SK112 or SK188. One of 46 (2%) subjects was identified as HTLV-I positive and 11 (24%) were identified as HTLV-II positive. Restriction fragment length polymorphism and sequence analyses indicated that all of the HTLV-II strains were subtype b. Mitochondrial DNA analyses indicated that all of the HTLV-II-positive subjects had an Amerindian haplotype. HTLV-II was more prevalent in Indians who were >45 years of age or female, had multiple sex partners or had received a blood transfusion. However, only the latter risk factor was statistically significant. Three of the HTLV-II-positive Indians demonstrated signs and symptoms of an ataxic neuropathy. The data support that HTLV-IIb is endemic among the Seminoles and that they will be a key population for further virologic studies.
authors.affiliation: Department of Epidemiology and Public Health, School of Medicine, University of Miami, Fla. 33136, USA.
authors.forename: G W
authors.initials: GW
authors.lastname: Lowis
authors.forename: W A
authors.initials: WA
authors.lastname: Sheremata
authors.forename: P R
authors.initials: PR
authors.lastname: Wickman
authors.forename: S
authors.initials: S
authors.lastname: Dube
authors.forename: K
authors.initials: K
authors.lastname: Dube
authors.forename: B J
authors.initials: BJ
authors.lastname: Poiesz
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017931:DNA Primers / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D005260:Female; D005431:Florida / Q000453:epidemiology; D015491:HTLV-II Infections / Q000208:ethnology* / Q000235:genetics; D006801:Humans; D007198:Indians, North American; D008297:Male; D008875:Middle Aged; D017354:Point Mutation / Q000235:genetics; D012307:Risk Factors
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial