Increment of nonreceptor tyrosine kinase Arg RNA as evaluated by semiquantitative RT-PCR in granulocyte and macrophage-like differentiation of HL-60 cells.
The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a myristoylated isoform. The C-terminal domains of Arg and c-abl, poorly similar to each other, may account for their different functions. Arg is cytoplasmic, c-abl also has nuclear localization, and their products have different transforming activity. To gain insight about the role of Arg in myeloid differentiation we investigated Arg gene expression in HL-60 cells differentiated with all-trans retinoic acid and 12-O-tetradecanoyl-phorbol-13-acetate. With a semiquantitative reverse transcriptase-polymerase chain reaction assay it was evident that the Arg transcript level in HL-60 cells differentiated toward granulocyte and macrophage-like lineage was, respectively, 3.5- and 2.8-fold the Arg level evidenced in undifferentiated HL-60 cells. In the HL-60 cells, under the same differentiating conditions, the c-abl RNA level did not change significantly, showing that Arg and c-abl responded in a different way to the inducers of differentiation used.
Perego
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RA
Institute of General Pathology, University of Milan and CNR Center for Research in Cellular Pathology, Milan, Italy. RAPEREGO@IMIUCCA.CSI.UNIMI.IT
Bianchi
C
C
Brando
B
B
Urbano
M
M
Del Monte
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U
D017209:Apoptosis; D002454:Cell Differentiation; D002455:Cell Division; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000378:metabolism*; D011505:Protein-Tyrosine Kinases / Q000235:genetics*; D012313:RNA; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology
D012333:RNA, Messenger; D014212:Tretinoin; D012313:RNA; D011505:Protein-Tyrosine Kinases; D013755:Tetradecanoylphorbol Acetate