Efficient presentation of multivalent antigens targeted to various cell surface molecules of dendritic cells and surface Ig of antigen-specific B cells.
To study the relation between the form of an Ag and the response to it, we compared presentation in vitro with hen egg lysozyme (HEL)-specific T cells from TCR transgenic mice of free HEL and liposome-encapsulated HEL by different APC. HEL-specific splenic B cells or bone marrow-derived dendritic cells were incubated with free HEL or HEL-containing liposomes targeted by Ab to either surface Ig, the Fc receptor, or MHC class I and II molecules. Ag presentation by HEL-specific B cells was at least 100-fold more efficient for HEL in surface Ig-targeted liposomes than free HEL taken up by the same receptor or HEL in liposomes targeted to class I or II molecules. Ag presentation by dendritic cells from Fc receptor-targeted vesicles was augmented 1,000-10,000-fold compared with free Ag or nontargeted liposomes, but presentation was also efficient when Ag was targeted to class I or II molecules. These results indicate that Ag-specific B cells and dendritic cells can be equally efficient in stimulating IL-2 production by Ag-specific T cells from unimmunized TCR transgenic mice when the Ag is multivalent and taken up by appropriate receptors. In contrast to B cells, which require engagement of surface Ig for optimal presentation, dendritic cells may present Ag by means of several different cell surface molecules.
Serre
K
K
Centre d'Immunologie de Marseille-Luminy, Marseille, France.
Machy
P
P
Grivel
J C
JC
Jolly
G
G
Brun
N
N
Barbet
J
J
Leserman
L
L
D000818:Animals; D017951:Antigen Presentation; D000954:Antigens, Surface / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001854:Bone Marrow Cells; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D018985:Epitopes, B-Lymphocyte / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis; D008024:Ligands; D008081:Liposomes / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*; D011961:Receptors, Fc / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism
D000954:Antigens, Surface; D018985:Epitopes, B-Lymphocyte; D007376:Interleukin-2; D008024:Ligands; D008081:Liposomes; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D009113:Muramidase