Spin-state selection filters for the measurement of heteronuclear one-bond coupling constants.
Novel alpha/beta-half-filter elements are proposed for the separation of the high-field and low-field component of 1JHC and 1JHN splittings into different subspectra. The alpha/beta-half-filter elements are of the same duration as the S3CT pulse sequence element and, like this, are less sensitive to cross talk between different subspectra than the original shorter alpha/beta-half-filters. The filter elements are demonstrated with the measurement of 1JHC coupling constants of C alpha H groups in 2D and 3D experiments and the subspectral editing of the four different multiplet components observed in two-dimensional alpha/beta-HSQC-alpha/beta spectra recorded without heteronuclear decoupling in either dimension.
Andersson
P
P
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Weigelt
J
J
Otting
G
G
D002247:Carbon Isotopes; D055598:Chemical Phenomena; D002621:Chemistry; D006859:Hydrogen / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
D002247:Carbon Isotopes; D006859:Hydrogen