Effects of NAD or NADP on the stability of liver and pectoral muscle enzymes in 3-acetylpyridine treated quail by heat and trypsin.
(1) The effects of long term treatment with 3-acetylpyridine on the stability of enzymes towards heat and trypsin treatment were studied. (2) In the liver NAD or NADP provided a similar degree of protection against heat inactivation at 55 degrees C for 6-phosphogluconate dehydrogenase (24%), glyceraldehyde-3-phosphate dehydrogenase (24%) and malic enzyme (20%), low level of protection of lactate dehydrogenase (13%) but didn't affect acetylcholinesterase at all. In the muscle, however, there was substantial protection against heat inactivation by coenzyme of glyceraldehyde-3-phosphate dehydrogenase (52%), an intermediate level of protection of lactate dehydrogenase (25%), low level of protection of 6-phosphogluconate dehydrogenase (17%) and malic enzyme (17%) and almost no protection of acetylcholinesterase. (3) In the susceptibility towards trypsin a low but similar degree of protection for dehydrogenases by coenzymes was observed in the liver whereas in the muscle there was substantial protection against trypsin inactivation by NAD of glyceraldehyde-3-phosphate dehydrogenase, an intermediate level of protection of 6-phosphogluconate dehydrogenase and malic enzyme and very little protection of lactate dehydrogenase but no protection of acetylcholinesterase. Among enzymes tested, glyceraldehyde-3-phosphate dehydrogenase showed the greatest protection against heat and trypsin inactivation by NAD. (4) The results suggest that the effect of 3-acetylpyridine treatment on the stability of muscle glyceraldehyde-3-phosphate dehydrogenase appears to be quite specific and selective.
Park
I K
IK
Department of Applied Biology, Dongguk University, Seoul, Korea. ikpark@cakra.dongguk.ac.kr
Kim
J Y
JY
D000818:Animals; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004795:Enzyme Stability / Q000187:drug effects; D005260:Female; D006358:Hot Temperature; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology*; D009249:NADP / Q000378:metabolism / Q000494:pharmacology*; D010369:Pectoralis Muscles / Q000187:drug effects / Q000201:enzymology*; D010734:Phosphogluconate Dehydrogenase / Q000187:drug effects / Q000378:metabolism; D011725:Pyridines / Q000494:pharmacology*; D011784:Quail; D014357:Trypsin / Q000378:metabolism / Q000494:pharmacology
D004791:Enzyme Inhibitors; D011725:Pyridines; C010835:3-acetylpyridine; D009243:NAD; D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D014357:Trypsin