title: Decreased expression of the interleukin 2 receptor on CD8 recipient lymphocytes in intestinal grafts rendered tolerant by liver transplantation in rats.
abstract: BACKGROUND
  In a previous study, it was shown that a spontaneously tolerated DA (RT1a) liver allograft in a PVG (RT1c) recipient was able to induce tolerance of a DA small bowel graft performed 17 days later in spite of infiltration of the intestinal grafts by mononuclear cells.
  
  
  OBJECTIVE
  To compare the phenotype of graft infiltrating cells in rejecting and tolerated small bowel grafts in order to elucidate the mechanism(s) which block the graft infiltrating cells from mediating rejection.
  
  
  METHODS
  Multiparameter immunofluorescence was used to compare the phenotype and state of activation of donor and recipient cells isolated from intestinal grafts rejected or tolerated after liver transplantation.
  
  
  RESULTS
  Three differences were found. Firstly, there was a more rapid replacement of lamina propria (LP) cells by recipient lymphocytes in tolerated than in rejected grafts. Secondly, the proportion of LP recipient CD8alphabeta+ lymphocytes bearing the high affinity receptor for interleukin 2 was significantly less in tolerated grafts (1.1%, range 0-2%) than in rejected grafts (21.3%, range 9-26%). Finally, tolerated grafts contained significantly less NK lymphocytes (NKR-P1+) and macrophages than rejected intestinal allografts.
  
  
  CONCLUSIONS
  These observations make it possible to delineate clear cut differences in the phenotype of cells infiltrating rejecting versus tolerated grafts. Furthermore, the data suggest that liver transplantation induces tolerance of intestinal grafts by hampering the activation of recipient TcRalphabeta+ CD8alphabeta+ T cells and subsequently the recruitment of non-specific effector cells.
authors.affiliation: Service de Chirurgie Pédiatrique, Faculté Necker Infants-Malades, 156, rue de Vaugirard, 75730 Paris Cedex 15, France.
authors.forename: S
authors.initials: S
authors.lastname: Sarnacki
authors.forename: H
authors.initials: H
authors.lastname: Nakai
authors.forename: D
authors.initials: D
authors.lastname: Calise
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: N
authors.initials: N
authors.lastname: Brousse
authors.forename: Y
authors.initials: Y
authors.lastname: Révillon
authors.forename: N
authors.initials: N
authors.lastname: Cerf-Bensussan
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D007150:Immunohistochemistry; D007421:Intestine, Small / Q000637:transplantation; D007694:Killer Cells, Natural / Q000378:metabolism; D016031:Liver Transplantation / Q000276:immunology*; D008264:Macrophages / Q000378:metabolism; D010641:Phenotype; D051381:Rats; D015375:Receptors, Interleukin-2 / Q000378:metabolism*; D014184:Transplantation, Homologous
chemical_list: D015375:Receptors, Interleukin-2