The human gene encoding the lectin-type oxidized LDL receptor (OLR1) is a novel member of the natural killer gene complex with a unique expression profile.
LOX-1 is an endothelial receptor for oxidized low-density lipoprotein that plays essential roles in atherogenesis. LOX-1 has the highest homology with C-type lectin receptors expressed on natural killer cells. In the present study, we cloned and characterized the human LOX-1 gene (HGMW-approved symbol OLR1). The gene structure of LOX-1 resembles that of the natural killer cell receptors. Fluorescence in situ hybridization and analyses of a yeast artificial chromosome contig revealed that the human LOX-1 gene is located in the natural killer gene complex on chromosome 12p12-p13, where the genes of the natural killer cell receptors cluster. In contrast, the expression pattern of LOX-1 is different from that of the natural killer cell receptors; LOX-1 is expressed in vascular-rich organs, but not in lymphocytes. A 1753-bp fragment of the 5' flanking region of the LOX-1 gene had a functional promoter activity. This region contains binding sites for several transcription factors, including the STAT family and NF-IL6, and the expression of LOX-1 was upregulated by several cytokines. These results demonstrate that the human LOX-1 gene is a new member of the natural killer gene complex with a unique expression profile.
Yamanaka
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S
Department of Pharmacology, Osaka City University Medical School, Osaka, 545-8585, Japan. syamanaka@med.osaka-cu.ac.jp
Zhang
X Y
XY
Miura
K
K
Kim
S
S
Iwao
H
H
D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000235:genetics; D001665:Binding Sites; D002874:Chromosome Mapping; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D002881:Chromosomes, Human, Pair 12 / Q000235:genetics; D003001:Cloning, Molecular; D016207:Cytokines / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007694:Killer Cells, Natural / Q000378:metabolism; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins / Q000235:genetics; D008969:Molecular Sequence Data; D051920:NK Cell Lectin-Like Receptor Subfamily D; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011973:Receptors, LDL / Q000737:chemistry / Q000235:genetics*; D051131:Receptors, Oxidized LDL; D015183:Restriction Mapping; D051127:Scavenger Receptors, Class E; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
D015703:Antigens, CD; D016207:Cytokines; D037181:Lectins, C-Type; D008562:Membrane Glycoproteins; D051920:NK Cell Lectin-Like Receptor Subfamily D; C495137:OLR1 protein, human; D012333:RNA, Messenger; D011973:Receptors, LDL; D051131:Receptors, Oxidized LDL; D051127:Scavenger Receptors, Class E; D014157:Transcription Factors