title: Improved antibiotic-resistance cassettes through restriction site elimination using Pfu DNA polymerase PCR.
authors.affiliation: Biotechnology Center for Agriculture and the Environment, Cook College, Rutgers University, New Brunswick 08901-8520, USA.
authors.forename: J J
authors.initials: JJ
authors.lastname: Dennis
authors.forename: G J
authors.initials: GJ
authors.lastname: Zylstra
mesh_terms: D001665:Binding Sites / Q000235:genetics; D002701:Chloramphenicol / Q000494:pharmacology; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004259:DNA-Directed DNA Polymerase; D004352:Drug Resistance, Microbial / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D017384:Sequence Deletion; D014295:Trimethoprim / Q000494:pharmacology
chemical_list: D004269:DNA, Bacterial; D007612:Kanamycin; D002701:Chloramphenicol; D014295:Trimethoprim; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes