title: Effect of immunotherapy on allergen-specific production of sulphidoleukotriene and histamine.
abstract: Numerous controlled trials have demonstrated the efficacy of specific immunotherapy, although its mechanism is not completely understood. Few studies have addressed the effects of immunotherapy on the release of mediators. We measured in vitro sulphidoleukotriene (sLT) and histamine release after specific stimulus (Dermatophagoides pteronyssinus or Lollium perenne) in a group of patients under immunotherapy (n = 35) and compared the results with those obtained in a group of allergic patients without immunotherapy (n = 57). SLT quantification was carried out by cellular stimulation allergen test (CAST)-ELISA and we measured the amount of histamine release using a fluorometric method. We found a significant (p < 0.05) reduction of allergen-specific mediator release on the group of patients under immunotherapy treatment. When we studied the group of patients sensitive to D. pteronyssinus we also observed a significant reduction in sLT release after the in vitro stimulus with anti-IgE. In vitro sLT production could be a good marker for follow-up immunotherapy. This study provides more evidence to support the immunological and cellular changes induced by immunotherapy.
authors.affiliation: Department of Allergy and Clinical Immunology, University Clinic, Faculty of Medicine, University of Navarra, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Ferrer
authors.forename: M L
authors.initials: ML
authors.lastname: Sanz
authors.forename: I
authors.initials: I
authors.lastname: Prieto
authors.forename: L
authors.initials: L
authors.lastname: Vila
authors.forename: A
authors.initials: A
authors.lastname: Oehling
mesh_terms: D000293:Adolescent; D000328:Adult; D000485:Allergens / Q000276:immunology / Q000627:therapeutic use; D000818:Animals; D003888:Desensitization, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015200:Fluoroimmunoassay; D006636:Histamine Release; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy*; D015289:Leukotrienes / Q000378:metabolism*; D008137:Longitudinal Studies; D008297:Male; D008925:Mites / Q000276:immunology
chemical_list: D000485:Allergens; D015289:Leukotrienes