Aggregation of recombinant hepatitis B surface antigen in Pichia pastoris.
The combination of immunoaffinity and size-exclusion chromatography (SEC) is a powerful tool to analyze multiprotein particle assembly. This approach was used to investigate the source of aggregation of recombinant hepatitis B surface antigen (HBsAg) detected in purified material. As HBsAg aggregation does not originate in the stresses, such as the concentration of HBsAg solutions, temperature and chaotropic agents, it is less probable that the HBsAg aggregate is produced during the process. To test whether aggregation takes place in vivo, crude yeast extract containing the expressed HBsAg was fractioned on a Sephacryl S-400 column just after cell disruption, and each fraction immunopurified individually. As a result, the HBsAg aggregate was isolated from a fraction corresponding to the elution of large particle aggregates only, not native HBsAg particles. It was biologically active, which demonstrates aggregate formation by specific assembly of partially or wholly folded HBsAg intermediates.
Tleugabulova
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D
Quality Control Department, National Center for Bioproducts, Havana, Cuba.
Falcón
V
V
Sewer
M
M
Pentón
E
E
D000911:Antibodies, Monoclonal; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D006514:Hepatitis B Surface Antigens / Q000737:chemistry* / Q000235:genetics; D008854:Microscopy, Electron; D010843:Pichia / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry*; D013861:Thiocyanates / Q000494:pharmacology
D000911:Antibodies, Monoclonal; D006514:Hepatitis B Surface Antigens; D011994:Recombinant Proteins; D013861:Thiocyanates; C009941:potassium thiocyanate