Overexpression of p185erbB2/neu in the NbE prostatic epithelial cell line increases cellular spreading and the expression of integrin alpha6beta1.
Overexpression of p185erbB2/neu has been demonstrated in approximately 30% of prostatic carcinomas and has been shown to induce tumorigenesis and metastasis in a prostatic epithelial cell line, NbE. To metastasize successfully, cells must be able to proliferate, degrade and be motile on a variety of substrates; thus attachment and spreading on a variety of substrates are key features of the metastatic phenotype. Using in vitro assays, we demonstrate that NbE-neuT-9/10 clones attached significantly better to specific substrates and spread significantly better on both specific and non-specific substrates as compared to the control clones. Additionally, the expression of integrin alpha6beta1, a key receptor enabling attachment and spreading on laminin, is upregulated on the metastatic clones NbE-neuT-9 and 10.
Vafa
A
A
Jim and Eilleen Dicke Research Laboratory, Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH, USA.
Zhang
Y
Y
Sikes
R A
RA
Marengo
S R
SR
D000704:Analysis of Variance; D002448:Cell Adhesion; D002465:Cell Movement; D004848:Epithelium / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D039121:Integrin alpha6beta1; D016023:Integrins / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D010641:Phenotype; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis* / Q000378:metabolism; D014407:Tumor Cells, Cultured
D039121:Integrin alpha6beta1; D016023:Integrins; D009363:Neoplasm Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases