title: Effect of controlled antigenic stimulation on lymphocyte subsets in pigs and pig fetuses.
abstract: The effect of controlled antigenic stimulation in immunologically virgin organisms, i.e. pig fetuses treated with NDCM (Nocardia delipidated cell mitogen) and germ-free (GF) piglets associated with a non-pathogenic E. coli 086, on peripheral blood lymphocyte subsets defined by the expression of CD5 and CD8 was studied by double color flow cytometry. Stimulation of both fetuses and GF piglets increased the frequency of CD8low+ lymphocytes. A prominent subset of CD5-CD8low+NK cells was present in GF and E. coli associated piglets and their frequency was slightly higher in E. coli associated animals. The most pronounced difference between stimulated and non-stimulated animals was in a relative proportion of an ill-defined lymphocyte subset with an unusual CD5low+CD8low+ expression. Both NDCM injection into fetal blood circulation and association of GF piglets with E. coli resulted in a marked increase of frequency of CD5low+CD8low+ lymphocytes in peripheral blood.
authors.affiliation: Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Reháková
authors.forename: J
authors.initials: J
authors.lastname: Sinkora
authors.forename: M
authors.initials: M
authors.lastname: Sinkora
authors.forename: H
authors.initials: H
authors.lastname: Kozáková
authors.forename: I
authors.initials: I
authors.lastname: Splíchal
authors.forename: R
authors.initials: R
authors.lastname: Barot-Ciorbaru
authors.forename: I
authors.initials: I
authors.lastname: Trebichavský
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D018956:CD5 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005333:Fetus; D005434:Flow Cytometry; D005856:Germ-Free Life; D007114:Immunization; D016130:Immunophenotyping; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000276:immunology*; D011247:Pregnancy; D013552:Swine
chemical_list: D000276:Adjuvants, Immunologic; D018956:CD5 Antigens; D016827:CD8 Antigens