Effect of hexachlorocyclohexane on hsp26 expression in transgenic Drosophila melanogaster.
Hexachlorocyclohexane (HCH) at different concentrations (0.5 to 5.0 ng/ml) mixed with food was fed to third instar larvae of hsp26-lacZ transgenic Drosophila for 2 hr and hsp26 gene expression was examined by beta-galactosidase staining. Puffing in salivary gland polytene chromosomes was also studied. Brain and midgut of larvae showed dark blue staining at 2.0 and 5.0 ng/ml of HCH. Absence of induced puffing at 67B and 84D in the salivary gland polytene chromosomes indicated that these glands are not affected. The study suggests presence of the vulnerable sites in larvae to the higher concentrations of HCH.
Chowdhuri
D K
DK
Embryotoxicology Section, Industrial Toxicology Research Centre, Lucknow, India. intox@itrc.sirnetd.ernet.in
Saxena
D K
DK
Viswanathan
P N
PN
D000818:Animals; D030801:Animals, Genetically Modified; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006360:Heat-Shock Proteins / Q000235:genetics*; D001556:Hexachlorocyclohexane / Q000494:pharmacology*
D029721:Drosophila Proteins; D006360:Heat-Shock Proteins; C087442:Hsp26 protein, Drosophila; D001556:Hexachlorocyclohexane