Determination of eprinomectin in plasma by high-performance liquid chromatography with automated solid phase extraction and fluorescence detection.
A method is described for the determination of eprinomectin in plasma using high-performance liquid chromatography with fluorescence detection (excitation and emission wavelengths 355 and 465 nm, respectively). The fluorescent derivative was obtained by a condensation reaction with trifluoroacetic anhydride and N-methylimidazole. The method employs 1 ml plasma samples and gives linear calibration graphs (r = 0.999) over the concentration range studied (0.5-50 ng ml-1). Solid extraction using the benchmate procedure was used for sample preparation. This method permits the determination of eprinomectin at levels as low as 0.1 ng ml-1 and is suitable for the pharmacokinetic study of eprinomectin in animals.
Sutra
J F
JF
Laboratoire de Pharmacologie-Toxicologie, INRA, Toulouse, France.
Chartier
C
C
Galtier
P
P
Alvinerie
M
M
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006041:Goats; D007306:Insecticides / Q000032:analysis*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry / Q000494:pharmacology
D007306:Insecticides; D007559:Ivermectin; C101434:eprinomectin