title: Rho-dependent cell spreading activated by E.coli cytotoxic necrotizing factor 1 hinders apoptosis in epithelial cells.
abstract: Cell-cell and cell-matrix interactions play a pivotal role in numerous cell functions including cell survival and death. In this work, we report evidence that the Rho-dependent cell spreading activated by a protein toxin from E. coli, the cytotoxic necrotizing factor 1 (CNF1), is capable of hindering apoptosis in HEp-2 cells. In addition to the promotion of cell spreading, CNF1 protects cells from the experimentally-induced rounding up and detachment and improves the ability of cells to adhere to each other and to the extracellular matrix by modulating the expression of proteins related to cell adhesion. In particular, the expression of integrins such as alpha 5, alpha 6 and alpha v, as well as of some heterotypic and homotypic adhesion-related proteins such as the Focal Adhesion Kinase, E-cadherin, alpha and beta catenins were significantly increased in cells exposed to CNF1. Our results suggest, however, that the promotion of Rho-dependent cell spreading is the key mechanism in protecting cells against apoptosis rather than cell adhesion per se. A toxin inducing cell spreading without activating Rho, such as Cytochalasin B, was in fact ineffective in favouring cell survival. These data are of relevance (i) for the understanding of the role of the actin-dependent and especially Rho-dependent cellular activities involved in apoptosis regulation and (ii) in providing some clues to understanding the mechanisms by which bacteria, by controlling cell fate, might exert their pathogenic activity.
authors.affiliation: Department of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena, Rome, Italy. fiorentini@ul.net.iss.it
authors.forename: C
authors.initials: C
authors.lastname: Fiorentini
authors.forename: P
authors.initials: P
authors.lastname: Matarrese
authors.forename: E
authors.initials: E
authors.lastname: Straface
authors.forename: L
authors.initials: L
authors.lastname: Falzano
authors.forename: G
authors.initials: G
authors.lastname: Donelli
authors.forename: P
authors.initials: P
authors.lastname: Boquet
authors.forename: W
authors.initials: W
authors.lastname: Malorni
mesh_terms: D000199:Actins / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D001427:Bacterial Toxins / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D003603:Cytotoxins / Q000494:pharmacology*; D004926:Escherichia coli / Q000472:pathogenicity; D029968:Escherichia coli Proteins; D019204:GTP-Binding Proteins / Q000502:physiology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D014466:Ultraviolet Rays; D020741:rho GTP-Binding Proteins
chemical_list: D000199:Actins; D000941:Antigens; D001427:Bacterial Toxins; D003603:Cytotoxins; D029968:Escherichia coli Proteins; C060296:cytotoxic necrotizing factor type 1; D003571:Cytochalasin B; D019204:GTP-Binding Proteins; D020741:rho GTP-Binding Proteins