title: Molecular analysis of regulation of gene expression of the human erythroid anion exchanger (AE) 1.
abstract: The anion exchange protein AE1 is the most abundant membrane protein in human erythrocytes mediating the electroneutral chloride/bicarbonate exchange. We identified a promoter region in the 5' flanking region of the human AE1 gene which controls transcription in a cell type independent manner. In addition a second, distal promoter element mediates gene expression only in erythroid cells and in dependence upon differentiation. Within this distal promoter region we defined a 44 bp sequence containing a novel CT-rich motif with very strong promoter activity whereas a second 28 bp segment suppresses gene expression.
authors.affiliation: Max-Planck-Institut für Biophysik, Frankfurt, Germany. stephan.philipp@med-rz.uni-sb.de
authors.forename: S
authors.initials: S
authors.lastname: Philipp
authors.forename: M
authors.initials: M
authors.lastname: Kneussel
authors.forename: J
authors.initials: J
authors.lastname: König
authors.forename: H
authors.initials: H
authors.lastname: Appelhans
mesh_terms: D017920:Antiporters / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D027963:Chloride-Bicarbonate Antiporters; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D020014:K562 Cells; D008081:Liposomes; D008156:Luciferases / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D006454:Hemoglobins; D008081:Liposomes; D011993:Recombinant Fusion Proteins; D008156:Luciferases