Serodiagnosis of Helicobacter pylori infection in Korean patients using enzyme-linked immunosorbent assay.
Helicobacter pylori (H. pylori) is a gram-negative spiral bacteria that are associated with gastritis, peptic ulcer and gastric cancer. We have developed enzyme-linked immunosorbent assay (ELISA) that detects serum anti-H. pylori immunoglobulin G antibodies using H. pylori strains isolated from Korean patients. To assess the sensitivity and specificity of our assay system with different commercial kits, serum samples from 249 Korean patients with a variety of gastrointestinal diseases were tested. Among 249 Korean patients, 178 (71.5%) were positive in culture and/or urease test. The sensitivity and specificity between our assay system and four other commercial kits (Bio-Rad, DAKO, ROCHE, and IPR) were as follows: 97.8% and 92%, 94.3% and 53%, 56.5% and 92%, 83.3% and 96%, 58.2% and 92%, respectively. All sera showing discordant immunoassay results between different ELISA kits were confirmed by immunoblot analysis. These results indicate that our assay system showed a highly accurate and reliable results in diagnosis of H. pylori infection in Korean patients.
Kim
S Y
SY
MOGAM Biotechnology Research Institute, Kyonggi-Do, Korea.
Ahn
J S
JS
Ha
Y J
YJ
Doh
H J
HJ
Jang
M H
MH
Chung
S I
SI
Park
H J
HJ
D000293:Adolescent; D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D002675:Child, Preschool; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004755:Enterobacteriaceae / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D005767:Gastrointestinal Diseases / Q000453:epidemiology / Q000382:microbiology; D016481:Helicobacter Infections / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology; D016480:Helicobacter pylori / Q000276:immunology; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007723:Korea / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G