New spectrophotometric procedure for determining cefotaxime based on derivatization with 1,2-naphthoquinone-4-sulphonate into solid-phase extraction cartridges--application to pharmaceutical and urine samples.
Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.
Martinez
L G
LG
Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, Burjassot/Valencia, Spain.
Campíns-Falcó
P
P
Sevillano-Cabeza
A
A
Bosch-Reig
F
F
D000328:Adult; D002439:Cefotaxime / Q000032:analysis* / Q000652:urine; D002511:Cephalosporins / Q000032:analysis* / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012680:Sensitivity and Specificity; D013053:Spectrophotometry / Q000379:methods*
D002511:Cephalosporins; D007202:Indicators and Reagents; D009285:Naphthoquinones; D004364:Pharmaceutical Preparations; C011942:1,2-naphthoquinone-4-sulfonate; D002439:Cefotaxime