title: Novel arterial pathology in mice and humans hemizygous for elastin.
abstract: Obstructive vascular disease is an important health problem in the industrialized world. Through a series of molecular genetic studies, we demonstrated that loss-of-function mutations in one elastin allele cause an inherited obstructive arterial disease, supravalvular aortic stenosis (SVAS). To define the mechanism of elastin's effect, we generated mice hemizygous for the elastin gene (ELN +/-). Although ELN mRNA and protein were reduced by 50% in ELN +/- mice, arterial compliance at physiologic pressures was nearly normal. This discrepancy was explained by a paradoxical increase of 35% in the number of elastic lamellae and smooth muscle in ELN +/- arteries. Examination of humans with ELN hemizygosity revealed a 2. 5-fold increase in elastic lamellae and smooth muscle. Thus, ELN hemizygosity in mice and humans induces a compensatory increase in the number of rings of elastic lamellae and smooth muscle during arterial development. Humans are exquisitely sensitive to reduced ELN expression, developing profound arterial thickening and markedly increased risk of obstructive vascular disease.
authors.affiliation: Cardiology Division and Program in Human Molecular Biology and Genetics, University of Utah Health Sciences Center, Salt Lake City, Utah 84112-5330, USA. dean@howard.genetics.utah.edu
authors.forename: D Y
authors.initials: DY
authors.lastname: Li
authors.forename: G
authors.initials: G
authors.lastname: Faury
authors.forename: D G
authors.initials: DG
authors.lastname: Taylor
authors.forename: E C
authors.initials: EC
authors.lastname: Davis
authors.forename: W A
authors.initials: WA
authors.lastname: Boyle
authors.forename: R P
authors.initials: RP
authors.lastname: Mecham
authors.forename: P
authors.initials: P
authors.lastname: Stenzel
authors.forename: B
authors.initials: B
authors.lastname: Boak
authors.forename: M T
authors.initials: MT
authors.lastname: Keating
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology* / Q000502:physiology; D001157:Arterial Occlusive Diseases / Q000235:genetics / Q000473:pathology* / Q000503:physiopathology; D015152:Blotting, Northern; D003187:Compliance; D004549:Elastin / Q000235:genetics*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D008854:Microscopy, Electron; D017540:Tunica Media / Q000473:pathology*
chemical_list: D004549:Elastin