Schizosaccharomyces pombe rad32 protein: a phosphoprotein with an essential phosphoesterase motif required for repair of DNA double strand breaks.
The Schizosaccharomyces pombe Rad32 protein is required for repair of DNA double strand breaks, minichromosome stability and meiotic recombination. We show here that the Rad32 protein is phosphorylated in a cell cycle-dependent manner and during meiosis. The phosphorylation is not dependent on the checkpoint protein Rad3. Analysis of a partially purified protein preparation indicates that Rad32 is likely to act in a complex. Characterisation of the rad32-1 mutation and site-directed mutagenesis indicate that three aspartate residues in the conserved phosphoesterase motifs are important for both mitotic and meiotic functions, namely response to UV and ionising radiation and spore viability.
Wilson
S
S
Department of Biochemistry, School of Biological Sciences, University of Sussex, Falmer, Brighton BN1 9QG, UK.
Tavassoli
M
M
Watts
F Z
FZ
D000251:Adenosine Triphosphatases / Q000502:physiology; D000483:Alleles; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution / Q000235:genetics; D001216:Asparagine / Q000235:genetics; D001224:Aspartic Acid / Q000235:genetics; D002850:Chromatography, Gel; D004249:DNA Damage; D004265:DNA Helicases / Q000502:physiology; D004260:DNA Repair / Q000187:drug effects / Q000528:radiation effects; D004950:Esterases / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D005090:Exodeoxyribonucleases; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016195:G2 Phase / Q000235:genetics; D006918:Hydroxyurea / Q000494:pharmacology; D007501:Iron / Q000378:metabolism; D008540:Meiosis; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016296:Mutagenesis; D010750:Phosphoproteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010766:Phosphorylation; D029701:Saccharomyces cerevisiae Proteins; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029702:Schizosaccharomyces pombe Proteins; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics / Q000502:physiology
D005656:Fungal Proteins; D010750:Phosphoproteins; D029701:Saccharomyces cerevisiae Proteins; D029702:Schizosaccharomyces pombe Proteins; D001224:Aspartic Acid; D001216:Asparagine; D008741:Methyl Methanesulfonate; D007501:Iron; D004950:Esterases; D005090:Exodeoxyribonucleases; C092453:Mre11 protein, S pombe; D000251:Adenosine Triphosphatases; C061897:Rad3 protein, S cerevisiae; D004265:DNA Helicases; D006918:Hydroxyurea