An anti-inflammatory component derived from milk of hyperimmunised cows reduces tight junction permeability in vitro.
OBJECTIVE
Effects of hyperimmune milk factor (HIMF), an anti-inflammatory factor from milk of hyperimmunised cows, on tight junction permeability and cell growth were studied in vitro.


METHODS
Mammary (HC11) and kidney (MDCK) epithelial cell lines were used.


METHODS
HIMF was used at a final concentration of 2 mg/ml.


METHODS
Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of HIMF with or without an inflammatory challenge. Cell growth was assessed by measuring total DNA of cultures with and without HIMF. Data were analysed by analyses of variance.


RESULTS
HIMF promoted tight junction formation and prevented loss of TER following a challenge in both epithelia. Post-challenge recovery of TER was also faster with HIMF. HIMF inhibited cell growth.


CONCLUSIONS
HIMF stimulates tight junction maintenance and formation, and its previously reported anti-inflammatory properties may be mediated by restricting the extravasation of white blood cells through tight junctions.
Stelwagen
K
K
Dairy Sciences, AgResearch, Ruakura Agricultural Centre, Hamilton, New Zealand. stelwagenk@agresearch.cri.nz
Ormrod
D J
DJ
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001428:Bacterial Vaccines / Q000276:immunology; D002417:Cattle / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004533:Egtazic Acid / Q000494:pharmacology; D017097:Electric Impedance; D004848:Epithelium / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D007114:Immunization; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008892:Milk / Q000737:chemistry*; D019108:Tight Junctions / Q000187:drug effects*
D000893:Anti-Inflammatory Agents; D001428:Bacterial Vaccines; D004533:Egtazic Acid