Chromosome landing at the barley Rar1 locus.
The barley Rar1 gene is an essential component of the race-specific, Mla-12-specified powdery mildew resistance reaction. As part of a map-based cloning strategy designed to isolate Rar1, five barley yeast artificial chromosomes (YACs) have been identified, ranging in size from 300 to 1100 kb. PCR-based YAC end-specific markers have been established and were employed to construct a local YAC contig. Four out of five YAC clones were found to be non-colinear with the source DNA. High-resolution genetic mapping of the YAC ends demonstrated that the set of five overlapping YAC clones encompasses the barley Rar1 gene.
Lahaye
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The Sainsbury Laboratory, John Innes Centre Norwich Research Park, UK.
Shirasu
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Schulze-Lefert
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D018244:Chromosomes, Artificial, Yeast; D003001:Cloning, Molecular; D020451:Contig Mapping; D016521:Electrophoresis, Gel, Pulsed-Field; D005658:Fungi / Q000472:pathogenicity; D015723:Gene Library; D017343:Genes, Plant; D001467:Hordeum / Q000235:genetics*; D010935:Plant Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012641:Selection, Genetic