title: Combined PCR/gapped-duplex method for site-directed mutagenesis.
authors.affiliation: Departamento de Imunologia, Universidade de São Paulo, Brazil.
authors.forename: L
authors.initials: L
authors.lastname: Isaac
authors.forename: C S
authors.initials: CS
authors.lastname: Farah
mesh_terms: D000818:Animals; D019556:COS Cells / Q000166:cytology / Q000378:metabolism; D003181:Complement C4 / Q000235:genetics; D004262:DNA Restriction Enzymes; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016297:Mutagenesis, Site-Directed; D009692:Nucleic Acid Heteroduplexes / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D011993:Recombinant Fusion Proteins / Q000235:genetics
chemical_list: D003181:Complement C4; D009692:Nucleic Acid Heteroduplexes; D011993:Recombinant Fusion Proteins; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004262:DNA Restriction Enzymes