Production of chimeric mice from cryopreserved blastocysts.
Mouse blastocysts derived from in vivo fertilization were cryopreserved by a simple vitrification method. Most of the cryopreserved blastocysts survived while demonstrating a normal morphology after warming (88.1%). The rate of cryopreserved blastocysts which developed to term as normal young was 56.8%, and chimeric mice were also produced from cryopreserved blastocysts injected with ES cells. As a result, 3.4% of the injected embryos developed to term as germ line chimeric mice.
Nakao
K
K
Center for Experimental Medicine, University of Tokyo, Japan.
Nakagata
N
N
Katsuki
M
M
D000818:Animals; D001755:Blastocyst / Q000502:physiology*; D002678:Chimera / Q000235:genetics*; D015925:Cryopreservation / Q000379:methods*; D005260:Female; D051379:Mice / Q000235:genetics*; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008845:Microinjections