Development of a scheme for genotyping Helicobacter pylori based on allelic variation in urease subunit genes.
Helicobacter pylori urease subunit genes in 383 isolates from 10 countries were investigated by PCR-restriction fragment length polymorphism (HaeIII) analysis. Eighty-two different ureAB profiles were documented by reference to known sequences. Variation among 51% of strains was accounted for by 10 predominant patterns, which provided a unique framework for categorizing isolates with geographically diverse origins.
Owen
R J
RJ
Laboratory of Enteric Pathogens, Central Public Health Laboratory, London, United Kingdom. rowen@phls.co.uk
Slater
E R
ER
Xerry
J
J
Peters
T M
TM
Teare
E L
EL
Grant
A
A
D000483:Alleles; D005798:Genes, Bacterial; D014644:Genetic Variation; D005838:Genotype; D016480:Helicobacter pylori / Q000145:classification* / Q000235:genetics; D006801:Humans; D012150:Polymorphism, Restriction Fragment Length; D014510:Urease / Q000235:genetics*
D014510:Urease