title: [Contribution of molecular biology to biotyping in epidemiological surveys of nosocomial infectious outbreaks].
abstract: During the period between the end of 1993 and the beginning of 1994 there was a large epidemic rush from severe nosocomial infection essentially in the form of septicemia. Authors implemented a classification of these epidemics and some strains were sent to the Institute Pasteur Paris for a further examination. These strains went under nuclear biology test (study of DNA plasmidic profiles and RNA ribosomal profiles) and passed through an identification by an acheiring biotypic system using 99 sources or carbones--biotype 99. These studies have shown that its about real epidemic, because the same strains were transmitted from one patient to another. This show the precarious condition of our hospital hygiene, involving the necessity of an efficient training of our health staff.
authors.affiliation: Hôpitaux des Armées, Dakar.
authors.forename: B
authors.initials: B
authors.lastname: Ndoye
authors.forename: L
authors.initials: L
authors.lastname: Hugard
mesh_terms: D000328:Adult; D015373:Bacterial Typing Techniques; D003428:Cross Infection / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D004269:DNA, Bacterial / Q000032:analysis; D004196:Disease Outbreaks; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D004754:Enterobacter / Q000145:classification / Q000302:isolation & purification; D004756:Enterobacteriaceae Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission; D006748:Hospital Departments; D006772:Hospitals, Military / Q000706:statistics & numerical data; D006779:Hospitals, Public / Q000706:statistics & numerical data; D006801:Humans; D006920:Hygiene; D007231:Infant, Newborn; D007234:Infant, Premature; D007709:Klebsiella / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000302:isolation & purification; D010564:Personnel, Hospital / Q000193:education; D010957:Plasmids / Q000235:genetics; D012675:Senegal / Q000453:epidemiology; D018805:Sepsis / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission
chemical_list: D004269:DNA, Bacterial
vernacular_title: Apport de la biologie moléculaire et de la biotypie dans l'enquête épidémiologique au cours des épidémies d'infections nosocomiales.