title: The Amazonia variant of Vibrio cholerae: molecular identification and study of virulence genes.
abstract: The pathogenic O1 Amazonia variant of Vibrio cholerae has been shown previously to have a cytotoxin acting on cultured Vero and Y-1 cells, and to lack important virulence factors such as the cholera toxin (Coelho et al. 1995a). This study extends the molecular analysis of the Amazonia strains, detecting the presence of the toxR gene, with a very similar sequence to that of the E1 Tor and classical biotypes. The outer membrane proteins are analyzed, detecting a variation among the group of Amazonia strains, with three different patterns found. As a by-product of this work a polymerase chain reaction fragment was sequenced, reading part of the sequence of the Lon protease of the Amazonia strains. This gene was not previously described in V. cholerae, but its sequence is present in the TIGR database specific for this species.
authors.affiliation: Departamento de Genética- I. Biologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Baptista
authors.forename: J R
authors.initials: JR
authors.lastname: Andrade
authors.forename: A C
authors.initials: AC
authors.lastname: Vicente
authors.forename: C A
authors.initials: CA
authors.lastname: Salles
authors.forename: A
authors.initials: A
authors.lastname: Coelho
mesh_terms: D049069:ATP-Dependent Proteases; D000595:Amino Acid Sequence; D001483:Base Sequence; D001938:Brazil; D002772:Cholera Toxin / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis* / Q000302:isolation & purification*; D005798:Genes, Bacterial / Q000235:genetics*; D006360:Heat-Shock Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D012697:Serine Endopeptidases / Q000235:genetics; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D002772:Cholera Toxin; D049069:ATP-Dependent Proteases; D012697:Serine Endopeptidases