A new technique of screening gene mutation--fluorescent fingerprinting.
A new technique called fluorescent fingerprinting (FF) was set up and used to screen and detect gene mutation from a large number of samples by using fluorescent labelled ddNTP and ABI-373A sequencer. By comparing the colour ladders of several lanes from the same sequencing region of different samples, any band with a different colour from its neighbours or a pattern displacement could be easily found out in the Macintosh screen which indicates a gene mutation. Then, the sequencing peak-chromatograms were made out only for the selected samples.
Liu
J
J
Basic Medical Research Center, Beijing Red Cross Chaoyang Hospital affiliate Capital University of Medical Sciences, People's Republic of China. Liujz@public.bta.net.cn
Xiang
H
H
Zhou
Y
Y
Zhu
Z
Z
Zhang
J
J
D016172:DNA Fingerprinting / Q000379:methods*; D005453:Fluorescence; D005456:Fluorescent Dyes; D005820:Genetic Testing; D009154:Mutation
D005456:Fluorescent Dyes