Fertilization of porcine oocytes following intracytoplasmic spermatozoon or isolated sperm head injection.
We demonstrated normal fertilization processes (as determined by pronuclear formation, pronuclear apposition and syngamy) in porcine oocytes either following intracytoplasmic spermatozoon (ICSI) or isolated sperm head injection. Microtubule organization and chromatin configuration were investigated in these oocytes during the first cell cycle. Following ICSI, the microtubular aster was organized from the neck of the spermatozoon and filled the whole cytoplasm. These male-derived microtubules appear to move both pronuclei to the center of oocytes. These cytoskeletal changes are analogous to those seen following conventional fertilization. In contrast, following isolated sperm head injection, the sperm aster was not seen. Instead, the microtubule matrix was organized from the cortex and then filled the whole cytoplasm in all cases in normally fertilized oocytes following injection (n=35). This organization is similar to what has been shown in the parthenogenetically activated oocytes. Chromosome analysis revealed that the oocytes injected with isolated sperm heads were fertilized normally. At 7 days following injection, the incidence of blastocoele formation following ICSI (38%) and isolated sperm head injection (22%) was higher than that following sham injection (2%). These results suggested that successful fertilization and preimplantation development occurred in porcine oocytes following either ICSI or isolated sperm head injection. Our results also indicated that fertilization processes can occur by self-assembled microtubules within cytoplasm in the absence of a sperm centrosome.
Kim
N H
NH
Animal Resource Research Center, Kon Kuk University, Seoul, Korea.
Lee
J W
JW
Jun
S H
SH
Lee
H T
HT
Chung
K S
KS
D000818:Animals; D003593:Cytoplasm; D005307:Fertilization in Vitro / Q000379:methods / Q000662:veterinary*; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000502:physiology*; D013077:Sperm Head / Q000502:physiology*; D013552:Swine / Q000502:physiology*; D015053:Zygote / Q000254:growth & development