title: Usefulness of surface phenotype study of intrathyroidal lymphocytes obtained by fine needle aspiration cytology in autoimmune thyroid disease and malignant lymphoma of the thyroid.
abstract: OBJECTIVE
  The surface phenotypes of intrathyroidal lymphocytes have been studied in various thyroid diseases. In most of the previous reports, intrathyroidal lymphocytes were obtained by surgical operation. We evaluated the usefulness of surface phenotype study of intrathyroidal lymphocytes obtained by fine needle aspiration in the diagnosis of Graves' disease, chronic thyroiditis, and malignant lymphoma of the thyroid.
  
  
  METHODS
  Eighty-seven untreated patients including 24 with Graves' disease, 59 with chronic thyroiditis, and 4 with malignant lymphoma of the thyroid, and 2 treated patients with malignant lymphoma of the thyroid were studied. Surface phenotypes of the peripheral lymphocytes and the intrathyroidal lymphocytes obtained by fine needle aspiration were analyzed using a FACScan and the monoclonal antibodies: anti-Leu5b/CD2, Leu4/CD3, Leu3a/CD4, Leu2a/CD8, and Leu12/CD19. Percentages of cells positive for each monoclonal antibody were calculated. In one case with malignant lymphoma, monoclonal antibodies to surface-immunoglobulin markers were also studied.
  
  
  RESULTS
  In peripheral lymphocytes, the percentage of positive cells in each phenotype was almost normal in each disease. In intrathyroidal lymphocytes, the percentage of CD19 positive cells was increased, and the percentage of CD2 and CD3 positive cells was reduced compared to those of peripheral lymphocytes in each disease. The percentage of intrathyroidal CD19 positive cells was remarkably high in malignant lymphoma, constituting more than 70% of cells. In Graves' disease, a relative decrease in the percentage of intrathyroidal CD4 positive cells and an increase in CD8 positive cells compared to peripheral lymphocytes were observed. In 2 treated patients with malignant lymphoma of the thyroid, the intrathyroidal CD19 positive cells almost disappeared in a patient shortly after successful treatment, and reappeared to constitute 76% of cells in a patient in relapse. In one case with malignant lymphoma in which monoclonal antibodies to surface-immunoglobulin markers were studied, the clonality of the affected cells could be demonstrated; more than 70% of the cells were positive for kappa light-chain and mu and delta heavy-chain. This finding was proved by subsequent immunohistochemical study based on open biopsy.
  
  
  CONCLUSIONS
  Surface phenotype study of intrathyroid lymphocytes obtained by fine needle aspiration has limited utility in the evaluation or diagnosis of Graves' disease and chronic thyroiditis. However, this simple rapid method is very helpful in the diagnosis and follow up of malignant lymphoma of the thyroid.
authors.affiliation: Second Department of Internal Medicine, Kyushu University, Fukuoka, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Fujikawa
authors.forename: K
authors.initials: K
authors.lastname: Okamura
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: T
authors.initials: T
authors.lastname: Mizokami
authors.forename: S
authors.initials: S
authors.lastname: Tanabe
authors.forename: H
authors.initials: H
authors.lastname: Ikenoue
authors.forename: S
authors.initials: S
authors.lastname: Okamura
authors.forename: M
authors.initials: M
authors.lastname: Ohta
authors.forename: M
authors.initials: M
authors.lastname: Fujishima
mesh_terms: D000328:Adult; D018941:Antigens, CD19 / Q000032:analysis; D001707:Biopsy, Needle; D018801:CD2 Antigens / Q000032:analysis; D017252:CD3 Complex / Q000032:analysis; D016516:CD4-CD8 Ratio; D003937:Diagnosis, Differential; D005260:Female; D005434:Flow Cytometry; D006111:Graves Disease / Q000175:diagnosis / Q000276:immunology; D006801:Humans; D016130:Immunophenotyping; D008214:Lymphocytes / Q000276:immunology; D008223:Lymphoma / Q000175:diagnosis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D013959:Thyroid Diseases / Q000175:diagnosis* / Q000276:immunology; D013961:Thyroid Gland / Q000276:immunology*; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000276:immunology; D013967:Thyroiditis, Autoimmune / Q000175:diagnosis / Q000276:immunology
chemical_list: D018941:Antigens, CD19; D018801:CD2 Antigens; D017252:CD3 Complex