title: Isolation and characterization of the Drosophila melanogaster cDNA encoding the sepiapterin reductase.
abstract: We have isolated and characterized the cDNA encoding Drosophila melanogaster sepiapterin reductase (SR). The amino acid sequence deduced from the cDNA sequence was 29% identical to those of mammalian SRs. The active site residues proposed from the three-dimensional structure of mouse SR are well conserved in Drosophila SR. The protein-coding region of the cDNA was expressed in Escherichia coli as a histidine fusion protein, and the resulting recombinant protein proved to have SR activity. The SR activity of the recombinant protein was inhibited by two indoleamines, N-acetyl serotonin and melatonin. Southern analysis suggests that the Drosophila SR gene is encoded by a single copy gene. RNA blot analysis revealed that the gene expresses 1.5 kb mRNA in both adult heads and bodies.
authors.affiliation: Department and Institute of Genetic Engineering, Kyung Hee University, Yongin City 449-701, South Korea.
authors.forename: C
authors.initials: C
authors.lastname: Seong
authors.forename: Y A
authors.initials: YA
authors.lastname: Kim
authors.forename: H J
authors.initials: HJ
authors.lastname: Chung
authors.forename: D
authors.initials: D
authors.lastname: Park
authors.forename: J
authors.initials: J
authors.lastname: Yim
authors.forename: K
authors.initials: K
authors.lastname: Baek
authors.forename: Y S
authors.initials: YS
authors.lastname: Park
authors.forename: K
authors.initials: K
authors.lastname: Han
authors.forename: J
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D000429:Alcohol Oxidoreductases; C022630:sepiapterin reductase