The function of adenosylcobalamin in the mechanism of ribonucleoside triphosphate reductase from Lactobacillus leichmannii.
Ribonucleoside triphosphate reductase from Lactobacillus leichmannii catalyzes the reduction of nucleotides to deoxynucleotides and uses adenosylcobalamin as a cofactor. A transient protein-based thiyl radical is essential for catalysis. Studies directed toward the elucidation of the function of adenosylcobalamin during catalysis have shown that formation of the thiyl radical and 5'-deoxyadenosine occurs in a concerted fashion with C-Co bond homolysis, that the homolysis is entropically and not enthalpically driven, that the dimethylbenzimidazole moiety of adenosylcobalamin is the axial ligand during catalysis, and that the C-Co bond is reformed after every turnover.
Lawrence
C C
CC
Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.
Stubbe
J
J
D000494:Allosteric Regulation / Q000502:physiology; D005075:Biological Evolution; D002384:Catalysis; D003035:Cobalt / Q000737:chemistry; D003038:Cobamides / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000096:biosynthesis; D004578:Electron Spin Resonance Spectroscopy; D019277:Entropy; D005609:Free Radicals / Q000378:metabolism; D007700:Kinetics; D007778:Lactobacillus / Q000201:enzymology*; D008024:Ligands; D016297:Mutagenesis, Site-Directed; D012264:Ribonucleotide Reductases / Q000737:chemistry / Q000378:metabolism*
D003038:Cobamides; D003854:Deoxyribonucleotides; D005609:Free Radicals; D008024:Ligands; D003035:Cobalt; D012264:Ribonucleotide Reductases; C000913:cobamamide