title: Human-derived cell lines to study xenobiotic metabolism.
abstract: In vitro systems make for rapid identification of xenobiotic effects and can be used to study cellular and subcellular toxicity mechanisms. In this report the metabolic competence of two human-derived cell lines, a hepatic (Hep G2) and a pulmonary one (A549) was tested. In the two cell systems the capability to activate Benzo[a]Pyrene through the cytochrome P450 enzyme system and to form reactive metabolites was analysed. 3H-BaP and the scintillation counting analysis were used to show the differences of the metabolic activity in Hep G2 and A549. A similar time course of 3H-BaP uptake was observed in the cell systems. Nevertheless, in the two cell lines the distribution of radioactive metabolites seemed to reflect a specific tissue response to toxicity.
authors.forename: C
authors.initials: C
authors.lastname: Urani
authors.forename: M
authors.initials: M
authors.lastname: Doldi
authors.forename: S
authors.initials: S
authors.lastname: Crippa
authors.forename: M
authors.initials: M
authors.lastname: Camatini
mesh_terms: D001564:Benzo(a)pyrene / Q000378:metabolism; D001711:Biotransformation; D015153:Blotting, Western; D002460:Cell Line; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D004785:Environmental Pollutants / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D014316:Tritium; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D015262:Xenobiotics; D014316:Tritium; D001564:Benzo(a)pyrene; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases