Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes.
An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.
Huang
C Y
CY
Department of Molecular and Integrative Physiology, University of Illinois, Urbana 61801, USA.
Kasai
M
M
Buetow
D E
DE
D000818:Animals; D004076:Digoxigenin; D007334:Insulin-Like Growth Factor I / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012313:RNA / Q000032:analysis*; D015347:RNA Probes; D012337:RNA, Ribosomal, 18S / Q000032:analysis; D051381:Rats; D013997:Time Factors
D015347:RNA Probes; D012337:RNA, Ribosomal, 18S; D012313:RNA; D007334:Insulin-Like Growth Factor I; D004076:Digoxigenin