Simultaneous determination of glucosamine and glucosamine 4-phosphate in Lipid A with high-performance anion-exchange chromatography (HPAEC).
A method for simultaneous determination of glucosamine (GlcN) and glucosamine 4-phosphate (GlcN-4-P) in Lipid A with high-performance anion-exchange chromatography (HPAEC) is described. Lipid A is hydrolyzed with 4 M HCl for 16 h at 100 degrees C, and the peaks of glucosamine and glucosamine 4-phosphate were measured. The true GlcN value can be computed from the GlcN value after correction for the incomplete hydrolysis of GlcN-4-P, or by the combined yield of GlcN and GlcN-4-P.
Kiang
J
J
Biology Department, Johns Hopkins University, Baltimore, MD 21218, USA.
Wang
L X
LX
Tang
M
M
Szu
S C
SC
Lee
Y C
YC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis*; D005958:Glucosephosphates / Q000032:analysis*; D008050:Lipid A / Q000737:chemistry*
D005958:Glucosephosphates; D008050:Lipid A; C055459:glucosamine 4-phosphate; D005944:Glucosamine