The mechanism of coupling of the organic anion exchange to Na(+)-dicarboxylate symport in basolateral membrane vesicles.
The rates of p-aminohippurate (PAH) and of uric acid uptake by basolateral membrane vesicles isolated from proximal tubules of rat kidney have been investigated. Accumulation of both substrates against the concentration gradient within the vesicles was shown to occur in the presence of alpha-ketoglutarate (alpha-KG) and Na+ gradient in the incubation medium. The mechanism of the coupling between Na(+)-dicarboxylate symport and organic anion transport is discussed based on the differences between the rates of PAH and of uric acid uptake. It is proposed that the limiting step of coupling between two transporters is the step of alpha-KG-organic anion exchange.
Rebane
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EN
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia.
Orlov YuN
Kazbekov
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EN
Lyubimov
Y a
Ya
Bulat
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MN
D000818:Animals; D027321:Anion Transport Proteins; D000838:Anions / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D016764:Cell Polarity; D019343:Citric Acid / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism; D005977:Glutarates / Q000378:metabolism; D017136:Ion Transport; D007523:Isocitrates / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D007687:Kidney Tubules, Proximal / Q000378:metabolism* / Q000648:ultrastructure; D062907:Oxaloacetic Acid / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism; D014527:Uric Acid / Q000378:metabolism*; D010130:p-Aminohippuric Acid / Q000378:metabolism*
D027321:Anion Transport Proteins; D000838:Anions; D002352:Carrier Proteins; D003998:Dicarboxylic Acids; D005650:Fumarates; D005977:Glutarates; D007523:Isocitrates; D007656:Ketoglutaric Acids; D015222:Sodium Channels; D014527:Uric Acid; D019343:Citric Acid; D062907:Oxaloacetic Acid; C032005:fumaric acid; D012964:Sodium; C034219:isocitric acid; D019802:Succinic Acid; C035736:glutaric acid; D010130:p-Aminohippuric Acid