title: Cytosolic lysophosphatidylcholine/transacylase in the production of dipolyunsaturated phosphatidylcholine in bonito muscle.
abstract: Phosphatidylcholine with docosahexaenoic acid at both sn-1 and sn-2 positions occurs in relatively high abundance in bonito muscle. To explore a possible route for the dipolyunsaturated molecular species, phosphatidylcholine formation from 2-[1-14C]linoleoyl lysophosphatidylcholine was examined using a cytosolic fraction from bonito muscle. The formation of radiolabeled phosphatidylcholine was greatest at 15 degrees C and did not require the presence of cofactors such as CoA and calcium. By DEAE-cellulofine column chromatography, the activity to form phosphatidylcholine was separated from that of phospholipase A1, and the specific activity increased by about 100-fold. The possible involvement of cytosolic lysophosphatidylcholine/transacylase in synthesis of dipolyunsaturated phosphatidylcholine is discussed.
authors.affiliation: Department of Food Science and Technology, Fukuyama University, Hiroshima, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: T
authors.initials: T
authors.lastname: Ito
authors.forename: H
authors.initials: H
authors.lastname: Morihara
authors.forename: T
authors.initials: T
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Satouchi
mesh_terms: D000215:Acylation; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000201:enzymology*; D004789:Enzyme Activation; D005399:Fishes; D008245:Lysophospholipase / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010741:Phospholipases A / Q000378:metabolism; D054466:Phospholipases A1; D014413:Tuna
chemical_list: D009097:Multienzyme Complexes; D010713:Phosphatidylcholines; D000217:Acyltransferases; D010741:Phospholipases A; D054466:Phospholipases A1; D008245:Lysophospholipase; C021782:lysophospholipase-transacylase