title: [Anti-Trichomonas activity of metronidazole in the presence of immunobiological preparations].
abstract: The minimal doses of metronidazole, capable of the irreversible immobilization of Trichomonas in the presence of one antiprotozoan preparation and after the addition of recombinant tumor necrosis factor (TNF), recombinant gamma-interferon and complex immunoglobulin preparation (CIP) into the culture medium, were determined on 4 T.vaginalis isolates, obtained on day 2 after the inoculation of material from the mucous membrane of the urogenital organs of patients (incubation at 37 degrees C). After 12-hour incubation of Trichomonas with immunobiological preparations their sensitivity to metronidazole increased 2-fold. After the simultaneous introduction of TNF, gamma-interferon and CIP into the culture medium, the sensitivity of Trichomonas to the action of metronidazole increased 5- to 7-fold. The introduction of an additional dose of TNF into the culture medium, but without CIP led to the preservation of the motility in a small number of these microorganisms. The use of human albumin instead of CIP at the same concentration (15 g/l) in the presence of TNF and gamma-interferon ensured the complete immobilization of Trichomonas in all isolates.
authors.affiliation: Institute of Engineering Immunology of RAO Biopreparat, Lyubuchany, Moscow Region, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Rubalskiĭ
authors.forename: Iu T
authors.initials: IuT
authors.lastname: Kalinin
authors.forename: S S
authors.initials: SS
authors.lastname: Afanas'ev
authors.forename: A A
authors.initials: AA
authors.lastname: Vorob'ev
authors.forename: G A
authors.initials: GA
authors.lastname: Dmitriev
authors.forename: L A
authors.initials: LA
authors.lastname: Denisov
authors.forename: E A
authors.initials: EA
authors.lastname: Khas'ianov
authors.forename: E E
authors.initials: EE
authors.lastname: Rubal'skaia
authors.forename: D S
authors.initials: DS
authors.lastname: Afanas'ev
authors.forename: M S
authors.initials: MS
authors.lastname: Afanas'ev
authors.forename: G Ia
authors.initials: GIa
authors.lastname: Shcherbakov
mesh_terms: D000818:Animals; D000994:Antitrichomonal Agents / Q000627:therapeutic use*; D003470:Culture Media; D004353:Drug Evaluation, Preclinical; D006801:Humans; D007116:Immunization, Passive; D007371:Interferon-gamma / Q000627:therapeutic use; D008795:Metronidazole / Q000627:therapeutic use*; D011994:Recombinant Proteins / Q000627:therapeutic use; D014246:Trichomonas vaginalis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000627:therapeutic use
chemical_list: D000994:Antitrichomonal Agents; D003470:Culture Media; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D008795:Metronidazole; D007371:Interferon-gamma
vernacular_title: Protivotrikhomonadnaia aktivnost' metronidazola v prisutstvii immunobiologicheskikh preparatov.