title: Cloning and nucleotide sequence of the alcohol acetyltransferase II gene (ATF2) from Saccharomyces cerevisiae Kyokai No. 7.
abstract: The ATF2 gene, which encodes alcohol acetyltransferase II (AATase II), was cloned from Saccharomyces cerevisiae Kyokai No. 7 (sake yeast). The ATF2 gene coded for a protein of 535 amino acid residues with a calculated molecular mass of 61,909 daltons. The deduced amino acid sequences of the ATF2 showed 36.9% similarity with that of ATF1, which encodes AATase I. The hydrophobicity profiles for the Atf2 protein and Atf1 protein were similar. A transformant carrying multiple copies of the ATF2 gene had 2.5-fold greater AATase activity than the control, and this activity was not significantly inhibited by linoleic acid. A Southern analysis of the yeast genomes in which the ATF2 gene was used as a probe showed that S. cerevisiae and brewery larger yeast have one ATF2 gene, while S. bayanus has no similar gene.
authors.affiliation: General Research Laboratory, Ozeki Corp., Hyogo, Japan. fvbb5555@mb.infoweb.ne.jp
authors.forename: N
authors.initials: N
authors.lastname: Nagasawa
authors.forename: T
authors.initials: T
authors.lastname: Bogaki
authors.forename: A
authors.initials: A
authors.lastname: Iwamatsu
authors.forename: M
authors.initials: M
authors.lastname: Hamachi
authors.forename: C
authors.initials: C
authors.lastname: Kumagai
mesh_terms: D000123:Acetyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000123:Acetyltransferases; C116217:ATF2 protein, S cerevisiae