title: Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24.
abstract: We present the cDNA sequence of human GBF1 along with data on expression pattern and genomic attributes. The deduced polypeptide encodes a putative guanine nucleotide exchange factor of 206.5 kDa, containing a centrally positioned Sec7 domain and a proline-rich region at the extreme C terminus. Its mRNA transcripts are found in 17 tissues and cell lines, likely suggesting a housekeeping role for GBF1p. The gene maps to 10q24, 2.33 cR from D10S540. It is fully contained within YAC 822C1 and covers at most 450 kb. Its Sec7 domain-encoding region harbors four introns.
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.
authors.forename: S J
authors.initials: SJ
authors.lastname: Mansour
authors.forename: J A
authors.initials: JA
authors.lastname: Herbrick
authors.forename: S W
authors.initials: SW
authors.lastname: Scherer
authors.forename: P
authors.initials: P
authors.lastname: Melançon
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10 / Q000235:genetics*; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D005656:Fungal Proteins; D020662:Guanine Nucleotide Exchange Factors; D011506:Proteins; D012333:RNA, Messenger; C066755:Sec7 guanine nucleotide exchange factors