ND-2001 suppresses lung metastasis of human renal cancer cells in athymic mice.
Explants of highly metastatic human renal cell carcinoma SN12Cpm6 cells in athymic mice were treated with sodium D-glucaro-delta-lactam (sodium 5-amino-5-deoxy-D-glucosaccharic acid-delta-lactam; ND-2001). ND-2001 (50 micrograms/ml) caused 78% inhibition of lung metastasis of SN12Cpm6 cells (two of five animals remaining metastasis free). The in vitro tumor cell invasion assay showed that ND-2001 (100 micrograms/ml) suppressed the invasive activity of SN12Cpm6 cells to Matrigel matrix at an inhibition rate of 72%. These results suggest that ND-2001 may be a new anti-metastatic drug against human cancer cells.
Kuramitsu
Y
Y
Laboratories of Pathology, Hokkaido University School of Medicine, Sapporo, Japan.
Hamada
J
J
Tsuruoka
T
T
Morikawa
K
K
Naito
S
S
Kobayashi
H
H
Hosokawa
M
M
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D005260:Female; D005937:Glucaric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009374:Neoplasms, Experimental / Q000188:drug therapy*
D000970:Antineoplastic Agents; C001845:D-glucaro-delta-lactam; D005937:Glucaric Acid