title: Fibrinogen/fibrin and fibronectin in the dentin-pulp complex after cavity preparation in rat molars.
abstract: OBJECTIVE
  The purpose of this study was to examine changes in distribution of fibrinogen/fibrin and fibronectin in the dentin-pulp complex after cavity preparation.
  
  
  METHODS
  Class V cavity preparations were prepared on maxillary first molars of 12 rats. The dentin and pulps were observed histologically and immunohistochemically for fibrinogen and fibronectin at 6 hours and 1, 2, and 3 days after the preparation.
  
  
  RESULTS
  At 6 hours and 1 day after cavity preparation, positive staining for fibrinogen was noted in the exudative lesion and in the dentinal tubules under the cavity preparation. Fibronectin staining in the exudate showed a pattern with close similarity to the fibrinogen staining. At 3 days after cavity preparation, the irregularly shaped predentin under the cavity preparation showed strong positive staining for fibronectin.
  
  
  CONCLUSIONS
  Fibrinogen/fibrin and fibronectin are present during the healing process of the dentin-pulp complex after cavity preparation.
authors.affiliation: Department of Conservative Dentistry, Fukuoka Dental College, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Izumi
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: K
authors.initials: K
authors.lastname: Watanabe
authors.forename: Y
authors.initials: Y
authors.lastname: Nishigawa
mesh_terms: D000818:Animals; D003737:Dental Cavity Preparation; D003782:Dental Pulp / Q000737:chemistry; D003804:Dentin / Q000737:chemistry*; D003809:Dentin, Secondary / Q000737:chemistry*; D019234:Dentinal Fluid / Q000737:chemistry; D005337:Fibrin / Q000032:analysis; D005340:Fibrinogen / Q000032:analysis* / Q000096:biosynthesis; D005353:Fibronectins / Q000032:analysis*; D007150:Immunohistochemistry; D009804:Odontoblasts / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D005353:Fibronectins; D005337:Fibrin; D005340:Fibrinogen