title: Use of capillary electrophoresis as a method development tool for classical gel electrophoresis.
abstract: Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan. These enantioseparation conditions were then applied to a classical gel electrophoresis system. Binding constants of the sulfated beta-cyclodextrin-piperoxan couple were approximated using CE and the effects of organic solvents on the system were also investigated.
authors.affiliation: Department of Chemistry, University of Cincinnati, OH 45221-0172, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Sutton
authors.forename: S R
authors.initials: SR
authors.lastname: Gratz
authors.forename: A M
authors.initials: AM
authors.lastname: Stalcup
mesh_terms: D002021:Buffers; D004586:Electrophoresis; D019075:Electrophoresis, Capillary; D007536:Isomerism; D010883:Piperoxan / Q000737:chemistry
chemical_list: D002021:Buffers; D010883:Piperoxan