Interleukin-10 abrogates the inhibition of Epstein-Barr virus-induced B-cell transformation by memory T-cell responses.
In vitro infection of human B lymphocytes by Epstein-Barr virus (EBV) results in their growth transformation and establishment of immortalized lymphoblastoid cell lines. The virus was found to encode a homologue of the pleiotropic cytokine interleukin-10 (IL-10), which has wide-ranging effects on the immune system. We investigated the effect of human IL-10 (hIL-10) and viral IL-10 (vIL-10) on EBV-specific immunological memory, as assessed by the inhibition of EBV-induced B-cell transformation by the autologous T cells. We found that IL-10 abrogates the inhibitory capacity of T cells. This IL-10 effect is mediated through suppression of T-cell activation-induced IL-2 and interferon-gamma production and through a direct enhancement of EBV-infected B-cell growth.
Bejarano
M T
MT
Microbiology and Tumor Biology Center, Karolinska Institutet, Stockholm, Sweden. maria.teresa.bejarano@mtc.ki.se
Masucci
M G
MG
D001402:B-Lymphocytes / Q000276:immunology* / Q000821:virology; D002472:Cell Transformation, Viral / Q000187:drug effects* / Q000276:immunology*; D002478:Cells, Cultured; D004854:Herpesvirus 4, Human; D006801:Humans; D007156:Immunologic Memory / Q000187:drug effects / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology* / Q000494:pharmacology; D008211:Lymphocyte Cooperation / Q000187:drug effects / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D016753:Interleukin-10