[Canine leishmaniasis from Leishmania infantum: value and production of the latex test. Ecoepidemiologic applications].
The authors relate the realization, evaluation and eco-epidemiological applications of a "field-test": the agglutination of latex particles coated with a soluble antigen of Leishmania infantum in the presence of homologous antibodies. Evaluated on 1,035 canine sera, the sensitivity of the latex agglutination test (LAT) was 93.4% compared to the indirect fluorescent antibody test (IFAT). 90 node cultures were carried out on dogs with positive or negative LAT and/or positive or negative IFAT. The frequency of positive node cultures (70%) as versus positive LAT came between the results obtained for an IFAT > 1/40 (64%) and IFAT > 1/80 (73%). 32/33 (97%) dogs had positive node culture, LAT and IFIAT(> 1/80). 6 dogs had negative LAT but positive node culture: 5 of these had also an IFAT < 1/160. This test was used in the field on several eco-epidemiological surveys in leishmanian enzootic areas. Node cultures were made on the dogs with positive TL. 39 strains were isolated: 18 in Algeria, 15 in Morocco, 2 in Syria and 4 in Yemen. 13/39 strains were obtained from dogs with IFI < 1/160: 2 at 1/20, 8 at 1/40 and 3 at 1/80. In Algeria this test was also used for the diagnosis of human visceral leishmaniasis in a child. This quick, simple, sensitive and specific test could be usefully carried out on "field" surveys for the diagnosis of visceral-leishmaniasis in animals and human beings.
Dereure
J
J
Laboratoire d'écologie médicale et de pathologie parasitaire, Faculté de médecine, Montpellier, France.
Lanotte
G
G
Pratlong
F
F
Gouvernet
J
J
Majhour
J
J
Belazzoug
S
S
Khiami
A
A
Rageh
H A
HA
Jarry
D
D
Périères
J
J
Rioux
J A
JA
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007841:Latex Fixation Tests; D018314:Leishmania infantum / Q000276:immunology* / Q000302:isolation & purification; D007898:Leishmaniasis, Visceral / Q000175:diagnosis / Q000662:veterinary*; D008198:Lymph Nodes / Q000469:parasitology; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan
Leishmaniose canine à Leishmania infantum: intérêt et réalisation du test au latex. Applications en éco-épidémiologie.