title: Molecular cloning and functional expression of the human glycine transporter GlyT2 and chromosomal localisation of the gene in the human genome.
abstract: Neurotransmitter transport systems are major targets for therapeutic alterations in synaptic function. We have cloned and sequenced a cDNA encoding the human type 2 glycine transporter GlyT2 from human brain and spinal cord. An open reading frame of 2391 nucleotides encodes a 797 amino acid protein that transports glycine in a Na+/Cl--dependent manner. When stably expressed in CHO cells, human GlyT2 displays a dose-dependent uptake of glycine with an apparent Km of 108 microM. This uptake is not affected by sarcosine at concentrations up to 1 mM. Radiation hybrid analysis mapped the GlyT2 gene to D11S1308 (LOD=8.988) on human chromosome 11p15.1-15.2.
authors.affiliation: Target Discovery Section, Organon Laboratories Limited, Newhouse, Lanarkshire, UK. j.morrow@organon.nhe.akzonobel.nl
authors.forename: J A
authors.initials: JA
authors.lastname: Morrow
authors.forename: I T
authors.initials: IT
authors.lastname: Collie
authors.forename: D R
authors.initials: DR
authors.lastname: Dunbar
authors.forename: G B
authors.initials: GB
authors.lastname: Walker
authors.forename: M
authors.initials: M
authors.lastname: Shahid
authors.forename: D R
authors.initials: DR
authors.lastname: Hill
mesh_terms: D000595:Amino Acid Sequence; D026921:Amino Acid Transport Systems, Neutral; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D016466:CHO Cells; D002352:Carrier Proteins / Q000235:genetics*; D002531:Cerebellum / Q000201:enzymology; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11; D003001:Cloning, Molecular; D006224:Cricetinae; D018076:DNA, Complementary / Q000032:analysis; D015870:Gene Expression; D015894:Genome, Human; D050487:Glycine Plasma Membrane Transport Proteins; D006801:Humans; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D012964:Sodium / Q000378:metabolism; D013116:Spinal Cord / Q000201:enzymology
chemical_list: D026921:Amino Acid Transport Systems, Neutral; D002352:Carrier Proteins; D018076:DNA, Complementary; D050487:Glycine Plasma Membrane Transport Proteins; C493271:SLC6A5 protein, human; D012964:Sodium