Relationship among serotype G3P5A rotavirus strains isolated from different host species.
The relationship among G3P5A rotavirus strains was analysed by restriction endonuclease assay of the VP4, VP6 and VP7 encoding genes, neutralization assay and phylogenetic analysis. The restriction patterns of the capsid encoding genes were species specific allowing the differentiation among the strains of different origin. The VP7 profiles differentiated human from animal strains more efficiently. The phylogenetic analysis of the VP4 gene demonstrated that HCR3A and K9 are closer related to each other than to other P5A strains. The same occurs to strains Ro1845 and Cat 97. The CU-1 virus appears to be an ancestor of the P5A strains by neutralization and phylogenetic analysis. The results placed the RRV strain definitely in a separate VP4 serotype and genotype from that of P5A strains. Restriction endonuclease assay of the capsid encoding genes seems to be a useful tool to identify the host species of rotavirus strains belonging to the same serotype and/or genotype.
Santos
N
N
Departamento de Virologia, Instituto de Microbiologia, RJ, 21941-590, Brazil. imvinos@microbio.ufrj.br
Clark
H F
HF
Hoshino
Y
Y
Gouvea
V
V
D000818:Animals; D000956:Antigens, Viral; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D005814:Genes, Viral / Q000235:genetics; D006801:Humans; D007223:Infant; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012703:Serotyping; D015678:Viral Structural Proteins / Q000235:genetics
D000956:Antigens, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; C060339:VP4 protein, Rotavirus; C061332:VP6 protein, Rotavirus; C050779:VP7 protein, Rotavirus; D015678:Viral Structural Proteins; D004262:DNA Restriction Enzymes