Cerebellar cell lines established from a p53-deficient adult mouse.
p53-Deficient mice are prone to develop spontaneous tumors [L.A. Donehower, M. Harvey, B.L. Slagle, M.J. McArthur, C.A. Montgomery Jr. , J.S. Butel, A. Bradley, Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumors, Nature 356 (1992) 215-221], but brain neoplasms are rare and difficult to culture in vitro. Here we describe cloning and long-term culture of heterogeneous neural cell lines from a multifocal cerebellar neoplasm that arose in an adult p53-/- mutant mouse. These lines may be useful for studies of neoplastic transformation and cell lineage in cerebellar development.
Minakawa
M
M
Department of Biological Science and Technology, Science University of Tokyo, 2641 Yamazaki Noda, Chiba 278-8510, Japan.
Sugimoto
T
T
Aizawa
S
S
Tomooka
Y
Y
D000818:Animals; D002528:Cerebellar Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008818:Mice, Neurologic Mutants; D014407:Tumor Cells, Cultured; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency*
D016159:Tumor Suppressor Protein p53