Pathways of beta-hydroxybutyrate contribution to metabolism of acetyl-CoA and acetylcholine in rat brain nerve terminals.
beta-hydroxybutyrate increased concentration of acetyl-CoA in mitochondria of resting and in cytoplasm of Ca-activated rat brain synaptosomes. Adequate rise of Ca-evoked acetylcholine release was also observed. The activation was abolished by verapamil. It indicates that beta-hydroxybutyrate-derived acetyl-CoA is transported from mitochondria to synaptoplasm by direct Ca-dependent transport mechanism. Presented data evidence that level of synaptoplasmic acetyl-CoA plays an important role in the regulation of cholinergic activity in the brain.
Tomaszewicz
M
M
Department of Clinical Biochemistry, School of Medicine, Gdańsk.
Bielarczyk
H
H
Jankowska
A
A
Szutowicz
A
A
D020155:3-Hydroxybutyric Acid / Q000494:pharmacology*; D000105:Acetyl Coenzyme A / Q000378:metabolism*; D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002799:Cholinergic Fibers / Q000187:drug effects / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects* / Q000378:metabolism
D000105:Acetyl Coenzyme A; D019289:Pyruvic Acid; D000109:Acetylcholine; D002118:Calcium; D020155:3-Hydroxybutyric Acid