The effect of endotoxin on in vivo rat alveolar macrophage phagocytosis.
This study was performed to explore whether alveolar macrophage (AM) phagocytosis would be impaired during endotoxemia. Therefore, we characterized in vivo AM phagocytic function in rats following either intravenous (i.v.) or intratracheal (i.t.) administration of lipopolysaccharide (LPS). The i.v. administration of LPS to rats at dosages of 0, 1, 2, and 5 mg/kg showed that increasing LPS doses were significantly associated with increased AM phagocytosis of 198Au colloid (P < .01), decreased recovery of AMs in bronchoalveolar lavage (BAL) (P = .017), no significant differences in neutrophil recovery by lavage (P = .15), or in the concentration of albumin in BAL (P = .14). Across the dosages of LPS administered i.t. (i.e., 0, 1, 5, and 10 mg/kg), there was no difference in AM phagocytosis (P = .29), a significant decrease in AM recovery (P = .002), a significant increase in neutrophil number (P = .01), and little effect on the concentration of albumin (P = .06). Thus, we found that the administration of endotoxin to rats did not impair in vivo AM phagocytic function. In fact, our findings suggest that the i.v. administration of LPS may increase AM phagocytosis of 198Au.
Frevert
C W
CW
Physiology Program, Harvard School of Public Health, Boston, Massachusetts, USA. cfrevert@u.washington.edu
Warner
A E
AE
Weller
E
E
Brain
J D
JD
D000418:Albumins / Q000032:analysis; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology; D002452:Cell Count; D004926:Escherichia coli; D017612:Gold Compounds / Q000378:metabolism / Q000494:pharmacology; D007275:Injections, Intravenous; D007442:Intubation, Intratracheal; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects* / Q000502:physiology; D051381:Rats
D000418:Albumins; D017612:Gold Compounds; D008070:Lipopolysaccharides