Antibody selection for immunohistochemical survey of equine tissue.
Immunohistochemical evaluation of equine tissue necessitates the use of antibodies reactive with cells from a heterogeneous population. Lymphoid tissues from 12 horses were fixed in Bouin's fluid, ethanol or formalin and examined for immunohistochemical reactivity with anti-equine and anti-human monoclonal antibodies (MAbs) specific for MHC Class II antigens, T and B lymphocytes, and macrophages. Only a few of the anti-equine MAbs tested were reactive with fixed, paraffin wax-embedded tissue. Anti-human MAbs expanded the desired range of reactivity and increased the consistency in different animals. The anti-equine MAbs conferred species specificity and anti-human MAbs provided an increased spectrum of reactivity.
Kalsow
C M
CM
Department of Ophthalmology, University of Rochester School of Medicine and Dentistry, New York 14642, USA.
Albrecht
T R
TR
Steinberg
N P
NP
Lunn
D P
DP
D019342:Acetic Acid / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000918:Antibody Specificity / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D000431:Ethanol / Q000276:immunology; D005557:Formaldehyde / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006736:Horses / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000276:immunology; D014066:Palatine Tonsil / Q000276:immunology; D010851:Picrates / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D016707:Tissue Fixation
D000911:Antibodies, Monoclonal; D000949:Histocompatibility Antigens Class II; D010851:Picrates; D005557:Formaldehyde; C026239:Bouin's solution; D000431:Ethanol; D019342:Acetic Acid