Adrenergic drug effects on cyclic AMP in cultured human trabecular meshwork cells.
Cyclic AMP production in the presence or absence of various adrenergic agonists and antagonists was determined in cultured human trabecular cells using a gammaflow automated radioimmunoassay that allowed multiple simultaneous experiments and reproducible results. Adrenergic agonists and antagonists showed activation and inhibition constants consistent with the presence of beta2-receptors: Ka of isoproterenol < epinephrine < norepinephrine < phenylephrine; Ki of timolol < betaxolol < celiprolol < atenolol. Selective ICI antagonists showed beta2-specificity.
Friedman
Z
Z
Annete and Rosin Eye Department, Bnai-Zion Medical Center and Technicon Faculty of Medicine, Haifa, Israel.
Bloom
E
E
Polansky
J R
JR
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000319:Adrenergic beta-Antagonists / Q000494:pharmacology*; D000328:Adult; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D010784:Photomicrography; D011863:Radioimmunoassay; D014129:Trabecular Meshwork / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology
D000318:Adrenergic beta-Agonists; D000319:Adrenergic beta-Antagonists; D000242:Cyclic AMP