title: Production of monoclonal antibodies specific for the i and 1,2 flagellar antigens of Salmonella typhimurium and characterization of their respective epitopes.
abstract: Salmonella typhimurium expresses two antigenically distinct flagellins, each containing a different H antigen (i and 1,2), the combination of which is highly specific for this serotype. In this study, overlapping recombinant flagellin fragments were constructed from the fliC (H:i) and fljB (H:1,2) flagellin genes, and the expression products were tested for binding to H antigen-specific monoclonal and polyclonal antibodies. A minimal area, 86 amino acids for H:i and 102 amino acids for H:1,2, located in the central variable domain of each flagellin was required for the binding of serotype-specific antibodies, providing further evidence for the presence of a discontinuous H epitope. Two peptides comprising these areas were shown to be highly suitable for application as antigens in an enzyme-linked immunosorbent assay detecting S. typhimurium-specific antibody.
authors.affiliation: Department of the Science of Food of Animal Origin, The Netherlands.
authors.forename: N
authors.initials: N
authors.lastname: de Vries
authors.forename: K A
authors.initials: KA
authors.lastname: Zwaagstra
authors.forename: J H
authors.initials: JH
authors.lastname: Huis in't Veld
authors.forename: F
authors.initials: F
authors.lastname: van Knapen
authors.forename: F G
authors.initials: FG
authors.lastname: van Zijderveld
authors.forename: J G
authors.initials: JG
authors.lastname: Kusters
mesh_terms: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins; D003001:Cloning, Molecular; D017931:DNA Primers; D000939:Epitopes / Q000032:analysis; D005408:Flagellin / Q000235:genetics / Q000276:immunology*; D005798:Genes, Bacterial; D006801:Humans; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012475:Salmonella / Q000276:immunology; D012486:Salmonella typhimurium / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D000939:Epitopes; C094427:FljB protein, Salmonella; D011994:Recombinant Proteins; D005408:Flagellin