Changes in bacterial species composition in enrichment cultures with various dilutions of inoculum as monitored by denaturing gradient gel electrophoresis.
Denaturing gradient gel electrophoresis revealed changes in the bacterial species obtained from enrichment cultures with different inoculum dilutions. This inoculum dilution enrichment approach may facilitate the detection and isolation of a greater number of bacterial species than traditional enrichment techniques.
Jackson
C R
CR
Department of Biological Sciences, University of Alabama, Tuscaloosa, Alabama 35487, USA. cjackson@biology.as.ua.edu
Roden
E E
EE
Churchill
P F
PF
D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018441:Biofilms; D004275:DNA, Ribosomal / Q000235:genetics*; D005618:Fresh Water; D008969:Molecular Sequence Data; D018515:Plant Leaves; D010944:Plants / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics* / Q000302:isolation & purification; D014871:Water Microbiology
D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S