title: Postnatal evolution of lymphocyte subpopulations in peripheral blood and lymphoid organs in the goat.
abstract: Monoclonal antibodies (mAbs) against bovine leukocyte antigens specific for T cells (CD2, CD4, CD8 and gammadelta receptor) and B cells (surface IgM) were used in samples from one week and one-, three- and seven-month-old goats to study the evolution of lymphocyte subsets by flow cytometry in peripheral blood and the lymphoid organs: thymus, jejunal (JPP) and ileal (IPP) Peyer's patches, spleen and mesenteric lymph nodes. An increase in the values of alpha/beta receptor T cells with age was recorded whereas the gammadelta receptor T cells fell in number. In peripheral blood and in all tissues, except IPP the values for B cells (sIgM+) were low. The CD4+ and CD8+ cells predominated in JPP while B cells were the most important subpopulation in IPP. In the spleen, as in JPP, the CD4/CD8 ratio was less than one and the gammadelta T cells values were high. In mesenteric lymph nodes, CD8+ and B(sIgM) cells predominated in the youngest animals.
authors.affiliation: Departamento de Patologia Animal (Microbiologia e Immunologia), Facultad de Veterinaria, Universidad de Murcia, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: Caro
authors.forename: M C
authors.initials: MC
authors.lastname: Gallego
authors.forename: A J
authors.initials: AJ
authors.lastname: Buendía
authors.forename: E
authors.initials: E
authors.lastname: Navarro
authors.forename: J A
authors.initials: JA
authors.lastname: Navarro
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis*; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D002417:Cattle; D005434:Flow Cytometry; D006041:Goats / Q000276:immunology*; D007082:Ileum / Q000254:growth & development / Q000276:immunology; D007583:Jejunum / Q000254:growth & development / Q000276:immunology; D008221:Lymphoid Tissue / Q000254:growth & development / Q000276:immunology*; D010581:Peyer's Patches / Q000254:growth & development / Q000276:immunology; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013950:Thymus Gland / Q000254:growth & development / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000945:Antigens, Differentiation, T-Lymphocyte; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta