A nonsymbiotic hemoglobin gene is expressed during somatic embryogenesis in Cichorium.
After differential screening of a cDNA library corresponding to genes expressed during the early stages of somatic embryogenesis in leaf tissue from the Cichorium hybrid '474' (C. intybus L., var. sativumxC. endivia L., var. latifolia) a nonsymbiotic hemoglobin cDNA was obtained. Studies of the expression of the gene corresponding to this clone by Northern blot analysis suggest that in Cichorium a nonsymbiotic hemoglobin gene is specifically expressed under somatic embryogenesis-inducing conditions, and that its expression is not related to stress caused by wounding or tissue culture conditions.
Hendriks
T
T
Laboratory of Plant Breeding, Agricultural University Wageningen, P. O. Box 386, 6700 AJ Wageningen, The Netherlands.
Scheer
I
I
Quillet
M C
MC
Randoux
B
B
Delbreil
B
B
Vasseur
J
J
Hilbert
J L
JL
D000595:Amino Acid Sequence; D001483:Base Sequence; D018651:Cichorium intybus / Q000196:embryology / Q000235:genetics*; D018076:DNA, Complementary / Q000032:analysis; D006454:Hemoglobins / Q000235:genetics*; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D018076:DNA, Complementary; D006454:Hemoglobins