Immediate early response of the p62 gene encoding a non-proteasomal multiubiquitin chain binding protein.
p62 is a cytoplasmic ubiquitin chain binding protein. Upon a variety of extracellular signals, both transcript and protein levels of p62 were rapidly increased. These signals include phorbol 12-myristate 13-acetate (PMA) and calcium ionomycin for peripheral blood mononuclear cells, serum or PDGF for serum-starved NIH3T3 cells, IL-3 for the G1 arrested pre-B cell line Ba/F3, and PMA for a human promyelocyte line U937. The elevation of p62 transcript level is due to temporal stabilization of mRNA and rapid activation of the p62 gene. Cycloheximide-induced enhanced transcription suggests the immediate early response of the p62 gene. The rapid induction of p62 indicates the presence of a novel ubiquitination-mediated regulatory mechanism during cell proliferation and differentiation.
Lee
Y H
YH
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
Ko
J
J
Joung
I
I
Kim
J H
JH
Shin
J
J
D016475:3T3 Cells; D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D001402:B-Lymphocytes; D002352:Carrier Proteins / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D017874:Immediate-Early Proteins / Q000096:biosynthesis / Q000097:blood / Q000235:genetics*; D007377:Interleukin-3 / Q000494:pharmacology / Q000502:physiology; D015759:Ionomycin / Q000494:pharmacology; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology / Q000502:physiology; D014176:Protein Biosynthesis / Q000187:drug effects; D011506:Proteins; D000071456:Sequestosome-1 Protein; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051758:Transcription Factor TFIIH; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects; D020298:U937 Cells; D014452:Ubiquitins / Q000378:metabolism*
D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D016895:Culture Media, Serum-Free; C490981:Gtf2h1 protein, mouse; D017874:Immediate-Early Proteins; D007377:Interleukin-3; D010982:Platelet-Derived Growth Factor; D011506:Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; D014157:Transcription Factors; D014452:Ubiquitins; D051758:Transcription Factor TFIIH; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate