Analysis of two incompletely spliced Arabidopsis cDNAs encoding novel types of peroxidase.
Most known class III peroxidase genes contain three introns at conserved positions. Two Arabidopsis cDNAs (ESTs), encoding novel type peroxidases ATP9a and ATP15a were sequenced, and found to contain inserts for intron 2. PCR and sequence analysis of genomic DNA revealed that the atp9a gene contains all three introns, whereas atp15a contains only introns 2 and 3. The ATP15a cDNA intron contained a single base substitution reducing the splicing potential significantly as compared with the genomic sequence. The putative enzymes share essential catalytic and structural features with horseradish peroxidase, despite a pair-wise sequence identity of only 40-45% among the three.
Justesen
A F
AF
Department of Protein Chemistry, Institute of Molecular Biology, University of Copenhagen, Oster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark.
Jespersen
H M
HM
Welinder
K G
KG
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018076:DNA, Complementary / Q000032:analysis*; D017343:Genes, Plant; D007438:Introns; D008969:Molecular Sequence Data; D009195:Peroxidase / Q000235:genetics*; D010544:Peroxidases / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D010544:Peroxidases; C116867:peroxidase ATP15a protein, Arabidopsis; C116866:peroxidase ATP9a protein, Arabidopsis; D009195:Peroxidase