Colicin N interaction with sensitive Escherichia coli cells: in situ and kinetic approaches.
The kinetics of colicin N binding to the Escherichia coli surface, comprising the recognition of and association with cell-surface-exposed sites of OmpF porin, is a rapid event taking place during the first seconds of incubation. Immunogold labelling demonstrates the membrane localization of the colicin N bound to sensitive cells. Analyses of colicin-induced efflux indicate a short lag before the onset of cytoplasmic K+ release. This delay reflects the time necessary for translocation from the external side and pore-forming insertion into the cytoplasmic membrane.
el Kouhen
R
R
Dept. of Pharmacology, Medical School Health Sciences, Minneapolis, MN 55455-0347, USA.
Bernadac
A
A
Pagès
J M
JM
D003087:Colicins / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007150:Immunohistochemistry; D007700:Kinetics; D018272:Porins / Q000378:metabolism; D011188:Potassium / Q000378:metabolism
D003087:Colicins; D018272:Porins; D011188:Potassium