Overexpression of chicken interferon regulatory factor-1 (Ch-IRF-1) induces constitutive expression of MHC class I antigens but does not confer virus resistance to a permanent chicken fibroblast cell line.
The chicken fibroblast cell line C32 has been transfected with the chicken homolog (Ch-IRF-1) of the mammalian transcription factor IRF-1. Stable transfectants were generated, constitutively overexpressing Ch-IRF-1 mRNA and protein. Cells overexpressing Ch-IRF-1 showed enhanced constitutive expression of MHC class I (B-F, beta-microglobulin) antigens. With increasing number of passages cells with normal B-F IV surface antigen expression accumulated. In the revertants, the amount of Ch-IRF-1 mRNA was reduced. Overexpression of Ch-IRF-1 had no effect on the constitutive expression and the induction by chicken interferon type-I and type-II (Ch-IFN) of guanylate-binding protein (GBP). Susceptibility to vesicular stomatitis virus, sindbis virus, Newcastle disease virus and vaccinia virus was not altered by overexpression of Ch-IRF-1. An antiviral state could be induced against all viruses tested by similar amounts of Ch-IFN type I in clone 20-18 expressing Ch-IRF-1 and cells transfected with empty vector.
Zoeller
B
B
Institute for Virology and Immunobiology, University of Würzburg, Versbacherstr. 7, D-97078, Würzburg, Germany. viro005@rzbox.uni-wuerzburg.de
Popp
M
M
Walter
A
A
Redmann-Müller
I
I
Lodemann
E
E
Jungwirth
C
C
D000818:Animals; D002352:Carrier Proteins / Q000235:genetics; D002460:Cell Line; D002645:Chickens; D018076:DNA, Complementary / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D015870:Gene Expression; D006157:Guanosine Monophosphate / Q000378:metabolism; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D050856:Interferon Regulatory Factor-1; D008322:Mammals; D009522:Newcastle disease virus / Q000276:immunology; D010750:Phosphoproteins / Q000235:genetics*; D012845:Sindbis Virus / Q000276:immunology; D014162:Transfection; D014616:Vaccinia virus / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D002352:Carrier Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D015395:Histocompatibility Antigens Class I; D050856:Interferon Regulatory Factor-1; D010750:Phosphoproteins; D006157:Guanosine Monophosphate