Ovarian activity in the scimitar-horned oryx (Oryx dammah) determined by faecal steroid analysis.
Ultrasonography and radioimmunoassay (RIA) of serum oestradiol-17beta, luteinizing hormone (LH) and progesterone, and faecal oestrogen and progestin was used to assess ovarian activity in the scimitar-horned oryx (Oryx dammah). Ovarian examination using ultrasonography revealed maximal follicle and corpus luteum (CL) diameters of 15 and 32 mm, respectively. Steroid hormone metabolite distribution among individual faecal pellets within the same defaecation was relatively homogeneous with coefficients of variation averaging 10.2+/-1.8% and 16.2+/-4.6% for oestrogens and progestins, respectively. Elevated faecal oestrogen concentrations were associated with large (> 10 mm) antral follicles detected by ultrasonography. Periovulatory peaks in faecal oestrogen excretion, coincident with nadirs in progestin excretion, were detected in three females. Faecal progestin excretion exhibited a similar temporal pattern to serum progesterone concentrations, with a time lag of approximately 16 h. Faecal progestin concentrations corresponded with the presence of functional CL and proved useful for monitoring luteal function, spontaneous and prostaglandin-F2alpha analogue-induced luteolysis and anovulation. In summary, faecal steroid monitoring is a practical, noninvasive method for characterising ovarian steroid excretion and has potential for facilitating the application of assisted reproductive technologies in scimitar-horned oryx.
Morrow
C J
CJ
Conservation and Research Center, National Zoological Park, Smithsonian Institution, Front Royal, VA 22630, USA. morrowc@agresearch.cri.nz
Monfort
S L
SL
D000818:Animals; D000866:Antelopes / Q000502:physiology*; D002851:Chromatography, High Pressure Liquid; D003338:Corpus Luteum / Q000000981:diagnostic imaging; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000032:analysis*; D005243:Feces / Q000737:chemistry*; D005260:Female; D007700:Kinetics; D007986:Luteinizing Hormone / Q000097:blood; D006080:Ovarian Follicle / Q000000981:diagnostic imaging; D010053:Ovary / Q000000981:diagnostic imaging / Q000502:physiology*; D011374:Progesterone / Q000097:blood / Q000493:pharmacokinetics; D011372:Progestins / Q000032:analysis*; D014463:Ultrasonography
D004967:Estrogens; D011372:Progestins; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone