Effect of procainamide on pulmonary thromboembolism and platelet malondialdehyde in mice.
OBJECTIVE
To study the effect of procainamide (PA) on pulmonary thromboembolism, platelet malondialdehyde (MDA) level and platelet aggregation induced by collagen and adrenaline.


METHODS
Pulmonary thromboembolism, 2-thiobarbituric acid fluorescence micro-determination, conventional microscopic counting, and platelet aggregation test were used.


RESULTS
PA (10-20 mg.kg-1) and mannitol (200 mg.kg-1) reduced thrombosis by 30%-75% and 75%, respectively. Thrombocytopenia followed thrombosis increased after the pretreatment of PA and mannitol. MDA decreased by both of them in vivo. In vitro, PA inhibited platelet aggregation and MDA production induced concentration-dependently by collagen and adrenaline.


CONCLUSIONS
Inhibition of PA on pulmonary thromboembolism is involved in the decrease of platelet aggregation and MDA production.
Pang
J X
JX
Department of Pharmacology, First Military Medical University, Guangzhou, China.
Sun
L S
LS
Wang
X Y
XY
Yang
S Q
SQ
Shan
C W
CW
D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D003094:Collagen; D004837:Epinephrine; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D051379:Mice; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011342:Procainamide / Q000494:pharmacology*; D011655:Pulmonary Embolism / Q000097:blood* / Q000139:chemically induced
D010975:Platelet Aggregation Inhibitors; D008315:Malondialdehyde; D003094:Collagen; D011342:Procainamide; D004837:Epinephrine