[Inhibitory effects of m-nifedipine on angiotensin II stimulated proliferation and protein synthesis in cultured rabbit aortic smooth muscle cells].
By using rabbit aortic smooth muscle cells (VSMC) in culture as a model, the effects of m-nifedipine (m-Nif) on the growth of VSMC were observed. The results showed that m-Nif inhibited [3H]-thymidine and [3H]-leucine incorporation into DNA and protein, decreased cell number in a concentration-dependent manner in angiotensin II (ANG II) induced VSMC. At the concentration of 2 mumol.L-1, m-Nif was found to reduce the ANG II (100 nmol.L-1) stimulated [3H]-thymidine, [3H]-leucine incorporation and cell number by 46%, 58% and 53%, respectively. Our results suggest that m-Nif can inhibit ANG II stimulated proliferation and protein synthesis in cultured rabbit aortic VSMC.
Gu
H B
HB
Department of Pathophysiology, Guangdong College of Pharmacy, Guangzhou.
Tang
Y H
YH
Xu
Y
Y
D000804:Angiotensin II / Q000037:antagonists & inhibitors*; D000818:Animals; D001011:Aorta / Q000166:cytology; D002121:Calcium Channel Blockers / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D007536:Isomerism; D009124:Muscle Proteins / Q000096:biosynthesis*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000378:metabolism; D009543:Nifedipine / Q000494:pharmacology*; D011817:Rabbits
D002121:Calcium Channel Blockers; D009124:Muscle Proteins; D000804:Angiotensin II; D009543:Nifedipine