[Indication and identification of California serogroup viruses in experimentally infected Ae. aegypti mosquitos by a lanthanum immunofluorescent analysis method].
The sensitivity and specificity of lanthanide fluoroimmunoassay (LFIA) and enzyme immunoassay (EIA) for indication and identification of California antigens in suspensions of infected Aedes aegypti mosquitoes are compared. The sensitivity of LFIA was 2.3 Ig TCD50/ml, EIA was less sensitive and detected virus antigens in mosquito suspensions at infective titer of 4.1 Ig TCD50/ml. If a pool contained mosquitoes infected with different California viruses, they could be identified only at the level of serological complex.
Pomelova
V G
VG
Lebedeva
S D
SD
Bychenkova
T A
TA
Khutoretskaia
N V
NV
Rasnitsyn
S P
SP
Osin
N S
NS
Butenko
A M
AM
D000330:Aedes / Q000821:virology*; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002141:Encephalitis Virus, California / Q000276:immunology / Q000302:isolation & purification*; D005260:Female; D005455:Fluorescent Antibody Technique; D008674:Metals, Rare Earth; D012680:Sensitivity and Specificity
D000956:Antigens, Viral; D008674:Metals, Rare Earth
Indikatsiia i identifikatsiia virusov kaliforniĭskoĭ serogruppy v éksperimental'no zarazhennykh komarakh Ae. aegypty metodom lantanidnogo immunofliuorestsentnogo analiza.