[Electron microscopic in situ hybridization of dUTP-digoxigenin labeled probes with polytene chromosomes of Drosophila melanogaster].
Using DNA probes labeled with digoxygenin-11-dUTP, a simplified method of electron microscopic (EM) in situ hybridization was developed for standard squashes of Drosophila melanogaster polytene chromosomes. The developed method is efficient and reproducible: its high resolution and specificity was shown for the transformed strain 148, in which the insertion was localized by EM as a new thin band. The method was applied for fine mapping of the developmentally regulated complex gene, muscleblind (mbl), which was shown to cover the 54B1-2 large band and the adjacent interbands in 2R polytene chromosome.
Semeshin
V F
VF
Institute of Cytology and Genetics, Siberain Branch of the Russian Academy of Sciences, Novosibirsk.
Artero
R
R
Peres-Alonso
M
M
Shloma
V V
VV
D000818:Animals; D002875:Chromosomes; D015342:DNA Probes; D003856:Deoxyuracil Nucleotides / Q000737:chemistry*; D004076:Digoxigenin / Q000737:chemistry*; D004331:Drosophila melanogaster / Q000235:genetics*; D017403:In Situ Hybridization; D008854:Microscopy, Electron; D015203:Reproducibility of Results
D015342:DNA Probes; D003856:Deoxyuracil Nucleotides; D004076:Digoxigenin
Elektronno-mikroskopicheskaia gibridizatsiia i situ prob, mechennykh dUTP-digoksigenninom, s politennymi khromosomami Drosophila melanogaster.