title: The transcriptional program in the response of human fibroblasts to serum.
abstract: The temporal program of gene expression during a model physiological response of human cells, the response of fibroblasts to serum, was explored with a complementary DNA microarray representing about 8600 different human genes. Genes could be clustered into groups on the basis of their temporal patterns of expression in this program. Many features of the transcriptional program appeared to be related to the physiology of wound repair, suggesting that fibroblasts play a larger and richer role in this complex multicellular response than had previously been appreciated.
authors.affiliation: Department of Biochemistry, Stanford University School of Medicine, Stanford CA 94305, USA.
authors.forename: V R
authors.initials: VR
authors.lastname: Iyer
authors.forename: M B
authors.initials: MB
authors.lastname: Eisen
authors.forename: D T
authors.initials: DT
authors.lastname: Ross
authors.forename: G
authors.initials: G
authors.lastname: Schuler
authors.forename: T
authors.initials: T
authors.lastname: Moore
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
authors.forename: J M
authors.initials: JM
authors.lastname: Trent
authors.forename: L M
authors.initials: LM
authors.lastname: Staudt
authors.forename: J
authors.initials: J
authors.lastname: Hudson
authors.forename: M S
authors.initials: MS
authors.lastname: Boguski
authors.forename: D
authors.initials: D
authors.lastname: Lashkari
authors.forename: D
authors.initials: D
authors.lastname: Shalon
authors.forename: D
authors.initials: D
authors.lastname: Botstein
authors.forename: P O
authors.initials: PO
authors.lastname: Brown
mesh_terms: D001769:Blood; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000235:genetics*; D002460:Cell Line; D002784:Cholesterol / Q000096:biosynthesis; D003470:Culture Media; D016895:Culture Media, Serum-Free; D020224:Expressed Sequence Tags; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation; D017781:Genes, Immediate-Early; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction / Q000379:methods; D012984:Software; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014945:Wound Healing / Q000235:genetics*
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D014157:Transcription Factors; D002784:Cholesterol; D017871:Calcium-Calmodulin-Dependent Protein Kinases