Effect of aminoguanidine on the generation of superoxide anion and nitric oxide by peripheral blood granulocytes of rats with streptozotocin-induced diabetes.
The effect of aminoguanidine (AG) on the production of superoxide anion O2*- and nitric oxide (NO) by peripheral blood granulocytes of rats with streptozotocin-induced diabetes was studied. Induction of diabetes resulted in an increase of O2*-. Generation by both unstimulated and opsonized zymosan-stimulated granulocytes was maximal after 6 weeks and lower after 12 weeks. Treatment with aminoguanidine (1 g/l drinking water) decreased O2*- generation after 6 weeks but not after 12 weeks. NO production by both unstimulated and opsonized zymosan-stimulated granulocytes was elevated in diabetic rats to a comparable extent after 6 and 12 weeks. AG attenuated this increased NO production. These results point to the beneficial effect of AG on oxidative stress in experimental diabetes and suggest an antioxidant effect of AG.
Kedziora-Kornatowska
K Z
KZ
Department of Internal Disease, Military Medical University, Lódz, Poland.
Luciak
M
M
Błaszczyk
J
J
Pawlak
W
W
D000818:Animals; D000838:Anions; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000097:blood*; D006442:Glycated Hemoglobin / Q000378:metabolism; D006098:Granulocytes / Q000187:drug effects* / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology*; D008297:Male; D009569:Nitric Oxide / Q000096:biosynthesis* / Q000097:blood; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D013481:Superoxides / Q000097:blood*
D000838:Anions; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006146:Guanidines; D009569:Nitric Oxide; D013311:Streptozocin; D013481:Superoxides; C004479:pimagedine