Reduced metastatic potential and c-myc overexpression of colon adenocarcinoma cells (Colon 26 line) transfected with nm23-R2/rat nucleoside diphosphate kinase alpha isoform.
Increased expression of nm23/nucleoside diphosphate kinase (NDP kinase) has been reported to be associated with both reduced metastatic potential in breast carcinoma and tumor progression in colon adenocarcinoma and lung adenocarcinoma. We examined effects of expression of nm23-R2 rat NDP kinase alpha isoform on mouse adenocarcinoma cells (Colon 26 line) and found a significant reduction of metastatic potential along with overexpression of c-myc. We also found that the proliferation rate of the transformed cells was the same as that of the control cells in culture. These results indicate that the cell growth potential in vitro is irrelevant to metastatic potential of the cells in vivo.
Tagashira
H
H
First Department of Surgery, Institute of Molecular and Cellular Biology, Okayama University Medical School, Okayama 700-8558, Japan.
Hamazaki
K
K
Tanaka
N
N
Gao
C
C
Namba
M
M
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology*; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D020559:Monomeric GTP-Binding Proteins; D054778:NM23 Nucleoside Diphosphate Kinases; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D051381:Rats; D014157:Transcription Factors / Q000235:genetics*; D014162:Transfection
D000951:Antigens, Neoplasm; D054778:NM23 Nucleoside Diphosphate Kinases; D016271:Proto-Oncogene Proteins c-myc; D014157:Transcription Factors; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins