title: [Screening of thermophilic superoxide dismatase producing strain and some properties of the enzyme].
abstract: A thermophilic SOD producing strain was obtained from the bacteria preserved in our lab. Its content of SOD was 8774u/g fresh cells. The strain can tolerate 0.4% H2O2 and 70 degrees C, and it has outstanding culture characteristics. It was identified as Bacillus stearothermophilus, called B.S 211-15. Crude SOD was extracted from B.S 211-15, the recovery of total activity was 69.5%, the specific activity was 1793u/mg protein. The enzyme showed fine heat stability, pH stability and good proteinase resistance. The SOD activity didn't decrease after being kept in room temperature for 2 months. The results of inhibition reactions indicated that this enzyme was Fe-SOD.
authors.affiliation: Department of Biotechnology, Sichuan University, Chengdu.
authors.forename: Z
authors.initials: Z
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: P
authors.initials: P
authors.lastname: Hu
mesh_terms: D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D013482:Superoxide Dismutase