Cationic trypsinogen produced by human pancreatic ductal cancer has the characteristics of spontaneous activation and gelatinolytic activity in the presence of proton.
We examined whether human pancreatic ductal cancer cells express and secrete pancreatic cationic trypsinogen in vitro which can be spontaneously converted into active trypsin at acidic pH (pH 4.5-5. 5), in contrast to anionic trypsinogen. Cationic trypsinogen expression at the mRNA level was observed in differentiated Capan-1 and BxPC-3 cell lines. However, expression was not detected in either poorly-differentiated Panc-1 or undifferentiated MIAPaCa-2 cell line. The gelatinolytic activity of the activated form of trypsinogen in each conditioned medium in the presence of enterokinase (1.0 microg/ml) (a band with a molecular weight of approximately 23 kDa) corresponded well to the level of cationic trypsinogen mRNA. The spontaneous activation of trypsinogen also was observed by gelatin zymography of the acid-loaded conditioned medium (pH 5.5). These findings suggest that trypsinogen produced by human pancreatic ductal cancer has the characteristics of spontaneous activation and gelatinolytic activity in the presence of proton.
Ohta
T
T
Department of Surgery (II), School of Medicine, Kanazawa University, Kanazawa 920, Japan.
Tajima
H
H
Fushida
S
S
Kitagawa
H
H
Kayahara
M
M
Nagakawa
T
T
Miwa
K
K
Yamamoto
M
M
Numata
M
M
Nakanuma
Y
Y
Kitamura
Y
Y
Terada
T
T
D017077:Culture Media, Conditioned; D005780:Gelatin / Q000737:chemistry / Q000378:metabolism; D005782:Gels; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010183:Pancreatic Ducts / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism; D014362:Trypsinogen / Q000235:genetics* / Q000378:metabolism*; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned; D005782:Gels; D005780:Gelatin; D014362:Trypsinogen; D014357:Trypsin