Role of endogenous endothelin peptides in intestinal ischemia-reperfusion injury in rats.
In this study, the effects of BQ123 (an ET(A) receptor antagonist), bosentan (a nonselective ET(A)-ET(B) antagonist), and phosphoramidon (an endothelin converting enzyme inhibitor) were investigated on intestinal mucosal lesion formation and changes in tissue PGE2 and LTC4 levels due to intestinal ischemia-reperfusion (I/R) injury in rats. Following 30 min of ischemia, the substances were given via the inferior caval vein, and 10 min later the intestine was subjected to reperfusion for 30 min. The intestinal specimens were evaluated both microscopically and the tissue PGE2 and LTC4 levels were obtained for each group. The histopathologic examination revealed a significant reduction in tissue injury in both BQ123 and phosphoramidon pretreated groups compared with the control group. Bosentan, on the contrary, did not decrease the injury. The pharmacologic examination revealed a significant reduction of PGE2-like activity in both BQ123 and phosphoramidon pretreated groups, compared with the control group, while LTC4-like activity remained unchanged except for an increase in the bosentan pretreated group.
Anadol
A Z
AZ
Department of General Surgery, Gazi University, Ankara, Turkey. hapkf@tr-net.net.tr
Bayram
O
O
Dursun
A
A
Ercan
S
S
D000818:Animals; D000077300:Bosentan; D015232:Dinoprostone / Q000378:metabolism; D065128:Endothelin Receptor Antagonists; D016232:Endothelins / Q000378:metabolism*; D005260:Female; D006020:Glycopeptides / Q000494:pharmacology; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000473:pathology; D007422:Intestines / Q000187:drug effects / Q000473:pathology*; D007511:Ischemia / Q000378:metabolism*; D017997:Leukotriene C4 / Q000378:metabolism; D008297:Male; D010456:Peptides, Cyclic / Q000494:pharmacology*; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism*; D013449:Sulfonamides / Q000494:pharmacology
D065128:Endothelin Receptor Antagonists; D016232:Endothelins; D006020:Glycopeptides; D010456:Peptides, Cyclic; D013449:Sulfonamides; D017997:Leukotriene C4; D015232:Dinoprostone; D000077300:Bosentan; C072247:cyclo(Trp-Asp-Pro-Val-Leu); C008890:phosphoramidon