Virulence properties of Escherichia coli 83972, a prototype strain associated with asymptomatic bacteriuria.
Little is known about bacteria associated with asymptomatic bacteriuria (ABU) with regard to urinary tract colonization mechanisms. In this study, virulence properties of Escherichia coli 83972, a strain that was isolated from a clinical ABU episode, were examined. The genetic potential for expression of P and type 1 pili was demonstrated, and DNA sequences related to type 1C and G (UCA) pilus genes were also detected. However, E. coli 83972 did not express D-mannose-resistant or D-mannose-sensitive hemagglutination after growth under standard conditions in vitro or upon isolation from the urine of colonized test subjects. Limited uroepithelial cell adherence was observed in vivo, and weak D-mannose-sensitive hemagglutination was detected after extended growth in urine in vitro.
Hull
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Department of Microbiology and Immunology, Baylor College of Medicine, University of Texas Health Science Center, Houston, Texas, USA. rhull@bcm.tmc.edu
Rudy
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Donovan
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Wieser
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Stewart
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Darouiche
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D018830:Adhesins, Escherichia coli / Q000235:genetics; D000483:Alleles; D001437:Bacteriuria / Q000382:microbiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D017422:Sequence Analysis, DNA; D001750:Urinary Bladder, Neurogenic / Q000382:microbiology; D014774:Virulence
D018830:Adhesins, Escherichia coli; C092426:PapG protein, E coli; D036781:Fimbriae Proteins