title: Marginal-zone B cells in the human lymph node and spleen show somatic hypermutations and display clonal expansion.
abstract: Splenic marginal-zone B cells, marginal-zone B cells of Peyer's patches in the gut, and nodal marginal-zone B cells (also identified as monocytoid B cells) share a similar morphology and immunophenotype. These cells likely represent a distinct subset of B cells in humans and rodents, but their precise ontogenetic relationship as well as their origin from B cells of the germinal center is still debated. To study this, we performed a mutation analysis of the rearranged immunoglobulin variable genes (VH) of microdissected single nodal and splenic marginal-zone cells. In addition, we investigated the presence of proliferating cells and B-cell clones in the human splenic and nodal marginal zone as well as adjacent germinal centers. This was performed by immunohistochemical staining for the Ki-67 antigen and denaturing gradient gel analysis of amplified immunoglobulin heavy chain genes' complementarity determining region 3 of microdissected cell clusters. A variable subset of nodal and splenic marginal-zone B cells showed somatic mutations in their rearranged VH genes, indicating that both virgin and memory B cells are present in the nodal and splenic marginal zone. Nodal and splenic marginal-zone B cells preferentially rearranged VH3 family genes such as DP47, DP49, DP54, and DP58. A preferential rearrangement of the same VH genes has been shown by others in the peripheral CD5(-) IgM+ B cells. These data suggest that the splenic and nodal marginal-zone B cells are closely related B-cell subsets. We also showed that marginal-zone B cells may cycle and that clones of B cells are frequently detected in the nodal as well as the splenic marginal zone. These clones are not related to those present in adjacent germinal centers. These data favor the hypothesis that clonal expansion occurs in the marginal zone. Whether the somatic hypermutation mechanism is activated during the clonal expansion in the marginal zone and which type of immune response triggers the clonal expansion need to be elucidated.
authors.affiliation: Departments of Pathology and Hematology, University Hospitals of Leuven, Leuven, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Tierens
authors.forename: J
authors.initials: J
authors.lastname: Delabie
authors.forename: L
authors.initials: L
authors.lastname: Michiels
authors.forename: P
authors.initials: P
authors.lastname: Vandenberghe
authors.forename: C
authors.initials: C
authors.lastname: De Wolf-Peeters
mesh_terms: D000595:Amino Acid Sequence; D001402:B-Lymphocytes / Q000166:cytology / Q000378:metabolism*; D002455:Cell Division / Q000276:immunology; D002469:Cell Separation; D002999:Clone Cells; D004252:DNA Mutational Analysis; D004210:Dissection; D004591:Electrophoresis, Polyacrylamide Gel; D015321:Gene Rearrangement / Q000276:immunology; D015326:Gene Rearrangement, B-Lymphocyte, Heavy Chain; D005803:Genes, Immunoglobulin; D018858:Germinal Center / Q000737:chemistry / Q000166:cytology; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000235:genetics; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000032:analysis; D008198:Lymph Nodes / Q000166:cytology* / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000166:cytology* / Q000378:metabolism
chemical_list: D007135:Immunoglobulin Variable Region; D019394:Ki-67 Antigen