Organic solvent tolerance of Escherichia coli is independent of OmpF levels in the membrane.
The organic solvent tolerance of Escherichia coli was measured under conditions in which OmpF levels were controlled by various means as follows: alteration of NaCl concentration in the medium, transformation with a stress-responsive gene (marA, robA, or soxS), or disruption of the ompF gene. It was shown that solvent tolerance of E. coli did not depend upon OmpF levels in the membrane.
Asako
H
H
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.
Kobayashi
K
K
Aono
R
R
D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D015870:Gene Expression; D005798:Genes, Bacterial; D010957:Plasmids / Q000235:genetics; D018272:Porins / Q000235:genetics / Q000378:metabolism*; D012997:Solvents; D014170:Transformation, Genetic
C109237:OmpF protein; D018272:Porins; D012997:Solvents