title: Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica.
abstract: The ACO3 gene, which encodes one of the acyl-CoA oxidase isoenzymes, was isolated from the alkane-utilizing yeast Yarrowia lipolytica as a 10 kb genomic fragment. It was sequenced and found to encode a 701-amino acid protein very similar to other ACOs, 67.5% identical to Y. lipolytica Aco1p and about 40% identical to S. cerevisiae Pox1p. Haploid strains with a disrupted allele were able to grow on fatty acids. The levels of acyl-CoA oxidase activity in the ACO3 deleted strain, in an ACO1 deleted strain and in the wild-type strain, suggested that ACO3 encodes a short chain acyl-CoA oxidase isoenzyme. This narrow substrate spectrum was confirmed by expression of Aco3p in E. coli.
authors.affiliation: Laboratoire de Génétique des Microorganismes, CNRS ERS567, INRA Centre de Grignon, Thiverval Grignon, France.
authors.forename: H
authors.initials: H
authors.lastname: Wang
authors.forename: A
authors.initials: A
authors.lastname: Le Clainche
authors.forename: M T
authors.initials: MT
authors.lastname: Le Dall
authors.forename: Y
authors.initials: Y
authors.lastname: Wache
authors.forename: Y
authors.initials: Y
authors.lastname: Pagot
authors.forename: J M
authors.initials: JM
authors.lastname: Belin
authors.forename: C
authors.initials: C
authors.lastname: Gaillardin
authors.forename: J M
authors.initials: JM
authors.lastname: Nicaud
mesh_terms: D042962:Acyl-CoA Oxidase; D000473:Alkanes / Q000378:metabolism; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D008830:Microbodies / Q000201:enzymology; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods; D015183:Restriction Mapping; D004718:Saccharomycetales / Q000201:enzymology / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D000473:Alkanes; D010088:Oxidoreductases; D042962:Acyl-CoA Oxidase