title: PCR-based random mutagenesis method using spiked oligonucleotides to randomize selected parts of a gene without any wild-type background.
authors.affiliation: Justus-Liebig Universität, Giessen, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Lanio
authors.forename: A
authors.initials: A
authors.lastname: Jeltsch
mesh_terms: D003001:Cloning, Molecular; D004259:DNA-Directed DNA Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific; D005820:Genetic Testing / Q000379:methods; D016297:Mutagenesis, Site-Directed; D015345:Oligonucleotide Probes; D010957:Plasmids; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D014170:Transformation, Genetic
chemical_list: D015345:Oligonucleotide Probes; C072146:Pfu DNA polymerase; D004259:DNA-Directed DNA Polymerase; D015252:Deoxyribonucleases, Type II Site-Specific; C027440:GATATC-specific type II deoxyribonucleases