FAD-mediated enzymatic conversion of NAD+ to NADH: application to chiral synthesis of L-lactate.
Electroenzymatic reduction of NAD+ to NADH for subsequent use in enzymatic synthesis has been carried out at carbon electrodes bearing lipoamide dehydrogenase (LiDH) immobilized under a Nafion film. The self-mediated electron transfer was made possible by an excess of flavin adenine dinucleotide (FAD) entrapped together with LiDH. Results were compared to those obtained with a similar electrode containing both LiDH and a polymeric form of FAD (pFAD) prepared by anodic polymerization of FAD.
Leonida
M D
MD
Fairleigh Dickinson University, Chemistry Department, Teaneck, NJ 07666, USA.
Sobolov
S B
SB
Fry
A J
AJ
D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D009243:NAD / Q000737:chemistry*
D004800:Enzymes, Immobilized; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D019344:Lactic Acid; D008058:Dihydrolipoamide Dehydrogenase