title: Experimental creation of fusiform carotid artery aneurysms using vein grafts in rats.
abstract: OBJECTIVE
  We developed an in vivo model of growing fusiform aneurysms, using vein grafts to the rat carotid artery. This aneurysm model might demonstrate the pathological features of the development and growth of aneurysms to become giant aneurysms.
  
  
  METHODS
  Placement of an interposed femoral vein graft to restore carotid artery flow was performed in Wistar rats. On Day 21, 75% of the grafts (mean diameter, 1.6 mm) were found to be dilated to resemble fusiform aneurysms (mean diameter, 5.82 mm), and 53% of these were giant. Quantitative analysis of the histological findings was performed using image-analyzing software.
  
  
  RESULTS
  Histological findings were similar to those for human intracranial giant aneurysms. The average length of the initial grafts in the aneurysm group was 9.1+/-1.9 mm, and grafts were significantly longer and more tortuous than in the normal graft group (6.4+/-0.8 mm) (P = 0.01). Cross-sectional areas of the aneurysms (mean, 18.9 mm2) were significantly correlated with the following: 1) the area of intra-aneurysmal thrombosis (mean, 11.1 mm2) (P < 0.0001); 2) the number of intrathrombotic vascular channels (P = 0.005); and 3) the area of dissection, with hemorrhage, between the thrombus and the wall of the aneurysm (mean, 0.72 mm2) (P = 0.0013). Scanning electron microscopic examination showed evidence of endothelial damage associated with growth of the aneurysms.
  
  
  CONCLUSIONS
  Recurrent hemorrhaging from intrathrombotic vascular channels caused dissection between the thrombus and the aneurysm wall, which led to growth of the experimental aneurysms to giant aneurysms. With this model, we demonstrated the growth mechanism of giant fusiform aneurysms.
authors.affiliation: Department of Neurosurgery, Nagoya University, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Fukui
authors.forename: M
authors.initials: M
authors.lastname: Negoro
authors.forename: H
authors.initials: H
authors.lastname: Keino
authors.forename: J
authors.initials: J
authors.lastname: Yoshida
mesh_terms: D000784:Aortic Dissection / Q000473:pathology; D000818:Animals; D019917:Blood Vessel Prosthesis Implantation; D002339:Carotid Arteries / Q000473:pathology; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D005268:Femoral Vein / Q000637:transplantation*; D002532:Intracranial Aneurysm / Q000473:pathology; D008297:Male; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017208:Rats, Wistar; D016042:Transplantation, Heterotopic