title: Monoclonal antibodies to the E2 protein of a new genotype (type 2) of bovine viral diarrhea virus define three antigenic domains involved in neutralization.
abstract: Bovine viral diarrhea virus (BVDV) has recently been segregated into two genotypes, namely, BVDV 1 and BVDV 2. Viruses of the BVDV 2 genotype are a cause of hemorrhagic and acute fatal disease in cattle in the US and Canada. In this study, monoclonal antibodies (mAbs) to the newly described BVDV 2 were produced after immunization with virus or a combination of virus and E2 peptide. From an original panel of 17 mAbs, 13 mAbs were identified as E2-specific by reactivity with a BVDV 2 recombinant E2 protein expressed in insect cells. Nine E2 mAbs were observed to be virus-neutralizing. The E2 epitopes represented by the mAbs were found to be highly conserved among BVDV 2 isolates associated with hemorrhagic or severe disease in cattle. Except for one virus-neutralizing E2 mAb, the mAbs showed few or relatively weak cross-reactions with BVDV 1. Two non-neutralizing E2 mAbs were BVDV 2-specific. In contrast to BVDV 1 for which conserved neutralizing epitopes have been mapped in one immunodominant domain, the virus-neutralizing E2 mAbs produced to BVDV 2 were found to bind to highly conserved epitopes in three antigenic domains.
authors.affiliation: Animal Diseases Research Institute, Canadian Food Inspection Agency, Lethbridge, Alberta.
authors.forename: D
authors.initials: D
authors.lastname: Deregt
authors.forename: P A
authors.initials: PA
authors.lastname: van Rijn
authors.forename: T Y
authors.initials: TY
authors.lastname: Wiens
authors.forename: J
authors.initials: J
authors.lastname: van den Hurk
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000302:isolation & purification; D000956:Antigens, Viral / Q000235:genetics*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000276:immunology / Q000821:virology; D002417:Cattle; D030241:Diarrhea Virus 1, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D030242:Diarrhea Virus 2, Bovine Viral / Q000145:classification / Q000235:genetics* / Q000276:immunology*; D018604:Epitope Mapping; D005838:Genotype; D016056:Immunodominant Epitopes / Q000235:genetics; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D014759:Viral Envelope Proteins; C076551:gp53, bovine viral diarrhea virus