title: Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells.
abstract: OBJECTIVE
  To study the influences of gomisin J on lipid peroxidation and calcium paradox.
  
  
  METHODS
  Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells.
  
  
  RESULTS
  Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) mumol.L-1, respectively, when cultured myocardial cells preincubated with Ca(2+)-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 mumol.L-1 protected myocardial cells through decreasing MDA formation.
  
  
  CONCLUSIONS
  Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injured by calcium paradox.
authors.affiliation: Department of Pharmacology, School of Pharmacy, Shanghai Medical University, China.
authors.forename: H L
authors.initials: HL
authors.lastname: Peng
authors.forename: D F
authors.initials: DF
authors.lastname: Chen
authors.forename: H X
authors.initials: HX
authors.lastname: Lan
authors.forename: X M
authors.initials: XM
authors.lastname: Zhang
authors.forename: Z
authors.initials: Z
authors.lastname: Gu
authors.forename: M H
authors.initials: MH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D017705:Lignans / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D017705:Lignans; D011083:Polycyclic Compounds; D008315:Malondialdehyde; C082091:gomisin J