title: Fibroblast growth factor-9 (glia-activating factor) stimulates proliferation and production of glial fibrillary acidic protein in human gliomas either in the presence or in the absence of the endogenous growth factor expression.
abstract: We tested fibroblast growth factor-9 (FGF-9) expression in human glioma cells (U251MG, T98G, U87MG, KALS-1, NMC-G1) and only NMC-G1 expressed endogenous FGF-9. All cells expressed bFGF and high affinity receptors for FGFs (FGFR1 and FGFR3). Exogenously supplied bFGF and FGF-9 both showed mitogenic activities in all cells. Neutralizing antibody against bFGF inhibited the proliferation in U251MG and NMC-G1, however that against FGF-9 inhibited the proliferation only in NMC-G1. GFAP expression was stimulated by both FGFs in these cells. FGF-9 potentially regulates proliferation and GFAP expression in human gliomas either in the presence or in the absence of the endogenous growth factor expression.
authors.affiliation: Department of Pathology, Kurume University, School of Medicine, Kurume, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Miyagi
authors.forename: S
authors.initials: S
authors.lastname: Kato
authors.forename: M
authors.initials: M
authors.lastname: Terasaki
authors.forename: T
authors.initials: T
authors.lastname: Aoki
authors.forename: Y
authors.initials: Y
authors.lastname: Sugita
authors.forename: M
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: M
authors.initials: M
authors.lastname: Shigemori
authors.forename: M
authors.initials: M
authors.lastname: Morimatsu
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D006133:Growth Substances / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D009363:Neoplasm Proteins; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors