title: [Retinol transport into the cell nucleus in vitro].
abstract: The retinol interaction with intact cellular nuclei, nuclear envelope and chromatin was investigated. We have shown that the Cellular Retinol-Binding Protein (CRBP) plays a very important role in such interaction. Retinol can specifically interacts with nuclei, nuclear envelope and chromatin only when it presents as a complex with CRBP. The obtained data allowed us to suggest that the process of retinol delivery to hypothetical nuclear receptors include of at least the following stages: 1) specific binding with nuclear envelope; 2) penetration into nuclei; 3) specific binding with chromatin receptors. CRBP is a necessary component at all this stages. Also we show that CRBP has not a species or tissue specificity.
authors.affiliation: A.V. Palladin Institute of Biochemistry, Kiev.
authors.forename: S V
authors.initials: SV
authors.lastname: Chetyrkin
authors.forename: L A
authors.initials: LA
authors.lastname: Chernukhina
authors.forename: G V
authors.initials: GV
authors.lastname: Donchenko
mesh_terms: D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D011485:Protein Binding; D012177:Retinol-Binding Proteins / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A / Q000378:metabolism*
chemical_list: D002843:Chromatin; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A
vernacular_title: Transport retinola v kletochnoe iadro in vitro.