Determination of Hb F levels: the routine methods.
The two classical methods used to determine the level of Hb F in a hemolysate are herein described. Measurement of this hemoglobin fraction by resistance to alkali denaturation was the first technique introduced; it is today largely replaced by ion exchange high performance liquid chromatography. The limits, pitfalls, and advantages of the two methods are discussed.
Préhu
C
C
Service de Biochimie, Hôpital Henri Mondor, Créteil, France.
Ducrocq
R
R
Godart
C
C
Riou
J
J
Galactéros
F
F
D000468:Alkalies / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis* / Q000276:immunology; D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans
D000468:Alkalies; D005319:Fetal Hemoglobin