title: Production of exfoliative toxin A by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk.
abstract: The production of exfoliative toxins A and B (ETA and ETB) by Staphylococcus aureus isolated from mastitic cow's milk and farm bulk milk was examined by the reverse passive latex agglutination method (RPLA). ETA was detected in 2 (1.2%) of 162 isolates from mastitic cow's milk and in 1 (0.6%) of 166 isolates from farm bulk milk. RPLA titers of these isolates were much lower than in human isolates. No ETB was detected in any of the isolates tested. These ETA-positive isolates belonged to bovine ecovar. They were non-typable using the international phage set for human strains. When these ETA-positive isolates were subcutaneously inoculated into neonatal mice, general exfoliation of the epidermis accompanied by the so-called Nikolsky sign was not recognized. By the immunoblotting and PCR methods, however, ETA and eta gene were recognized in the ETA-positive isolates from mastitic cow's milk and farm bulk milk. These data suggest that ETA is also produced by bovine isolates of S. aureus, but in smaller quantities.
authors.affiliation: Hokubu Livestock Hygiene Service Center, Ishikawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hayakawa
authors.forename: M
authors.initials: M
authors.lastname: Hayashi
authors.forename: T
authors.initials: T
authors.lastname: Shimano
authors.forename: H
authors.initials: H
authors.lastname: Komae
authors.forename: K
authors.initials: K
authors.lastname: Takeuchi
authors.forename: M
authors.initials: M
authors.lastname: Endou
authors.forename: H
authors.initials: H
authors.lastname: Igarashi
authors.forename: N
authors.initials: N
authors.lastname: Hashimoto
authors.forename: S
authors.initials: S
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D002417:Cattle; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005083:Exfoliatins / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques / Q000662:veterinary; D007841:Latex Fixation Tests / Q000662:veterinary; D008414:Mastitis, Bovine / Q000382:microbiology*; D051379:Mice; D008892:Milk / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013211:Staphylococcus aureus / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D005083:Exfoliatins