Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes.
A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60 min of incubation, 80.2 +/- 2.8% of swine granulocytes and 77.0 +/- 2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5 +/- 3.2% of human granulocytes and in 92.8 +/- 3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different.
Busque
P
P
Department of Pathology and Microbiology, College of Veterinary Medicine, University of Montreal, Quebec, Canada.
Higgins
R
R
Sénéchal
S
S
Marchand
R
R
Quessy
S
S
D000818:Animals; D005434:Flow Cytometry; D006098:Granulocytes / Q000382:microbiology / Q000502:physiology; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000382:microbiology / Q000502:physiology*; D009000:Monocytes / Q000382:microbiology / Q000502:physiology; D009504:Neutrophils / Q000382:microbiology / Q000502:physiology*; D010587:Phagocytosis; D012703:Serotyping; D017011:Streptococcus suis / Q000145:classification; D013552:Swine