"Cut and combine": an easy membrane-supported combinatorial synthesis technique.
A combinatorial synthesis process involving sequential cycles of cutting a membrane support into pieces and combining these into groups and subjecting the groups to simultaneous solid-phase chemical reactions is demonstrated by the rapid assembly of four hundred N-terminally biotinylated, soluble, octameric peptide pools. Index patterns printed onto the synthesis membrane allowed a direct identification of the compounds. These were used to study protein kinase substrate selection in a parallel microplate adapted 32P-phosphorylation assay with subsequent spotting on a biotin-capture membrane.
Dittrich
F
F
AG Molecular Recognition, GBF, Braunschweig, Germany.
Tegge
W
W
Frank
R
R
D000595:Amino Acid Sequence; D019921:Biotinylation; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D015195:Drug Design; D007202:Indicators and Reagents; D008958:Models, Molecular; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D010209:Paper; D019151:Peptide Library; D011487:Protein Conformation; D019809:Streptavidin; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D007202:Indicators and Reagents; D009842:Oligopeptides; D019151:Peptide Library; D019809:Streptavidin; D017868:Cyclic AMP-Dependent Protein Kinases