[Production of human insulin from proinsulin by a biological catalysis].
The conversion of porcine and human proinsulins to insulin by enzymatic hydrolysis induced by trypsin and carboxypeptidase B was studied. The conditions of reactions in the presence of trypsin (pure or immobilized in PAAG) and carboxypeptidase B were determined. The possibility of insulin production by the use of immobilized trypsin and carboxypeptidase B for practical purposes was shown.
Ivankin
A N
AN
All-Russia Institute of Meat Industry, Moscow, Russia.
Nekliudov
A D
AD
Mitaleva
S I
SI
D043424:Carboxypeptidase B; D002268:Carboxypeptidases; D002384:Catalysis; D006801:Humans; D006868:Hydrolysis; D007328:Insulin / Q000737:chemistry*; D011384:Proinsulin / Q000737:chemistry*; D014357:Trypsin
D007328:Insulin; D011384:Proinsulin; D002268:Carboxypeptidases; D043424:Carboxypeptidase B; D014357:Trypsin
Poluchenie insulina cheloveka ix proinsulina biokataliticheskim metodom.