[cDNA library construction from compacted eight-cell mouse embryos and differential screening for specific-expressed cDNA clones].
A large and representative cDNA library containing 6.9 x 10(5) independent clones was constructed from about 2 x 10(3) mouse embryos at the compacted 8-cell stage to identify and characterize gene products which play crucial roles in the first differentiation process of mouse embryogenesis. The cDNA library was differentially screened with labelled cDNA probes synthesized on poly (A)+ RNA isolated from the compacted 8-cell morula or late 2-cell mouse embryos. The results showed that difference in gene expression existed between these two developmental stages. Two clones which appeared only at the 8-cell stage were selected. This study provides a valuable tool for further detailed analysis of specific proteins associated with developmental events.
Chen
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L
Human Reproductive Engineering Laboratory, Hunan Medical University.
Lu
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G
Lu
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H
D000818:Animals; D002999:Clone Cells; D018076:DNA, Complementary; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D015723:Gene Library; D008297:Male; D051379:Mice
D018076:DNA, Complementary