Fibronectin and laminin binding of urogenital and oral prevotella species.
88 strains of five Prevotella species--P. bivia, P. buccae, P. disiens, P. oralis, and P. oris--were examined for their fibronectin and laminin binding properties with the aid of latex particle agglutination assays. Beside single protein binding activities, all species showed strains that adhered to both fibronectin and laminin. The oral species, P. buccae, P. oralis, and P. oris were found to interact with laminin to a pronouncedly higher extent than with fibronectin. The urogenital species, P. bivia and P. disiens showed comparable activities of binding to fibronectin and laminin, with P. bivia exhibiting higher matrix protein binding rates than P. disiens. Within the oral species group, P. oralis showed a higher percentage of fibronectin and laminin reactive strains than did P. buccae and P. oris. The finding of species-related different binding properties may throw some light on the known differences in clinical relevance and pathogenicity of the urogenital species, P. bivia and P. disiens, but does so only in part concerning the oral species, P. buccae, P. oralis, and P. oris. Moreover, the observed differences in matrix protein binding of Prevotella species may have implications in chemotaxis and opsonization on the one hand and maintenance of colonization activities under antibiotic therapy on the other.
Eiring
P
P
University of Tübingen, Department of Medical Microbiology, Germany.
Waller
K
K
Widmann
A
A
Werner
H
H
D016866:Bacteroidaceae Infections / Q000382:microbiology*; D052776:Female Urogenital Diseases / Q000382:microbiology; D005353:Fibronectins / Q000378:metabolism*; D006801:Humans; D007797:Laminin / Q000378:metabolism*; D007841:Latex Fixation Tests; D052801:Male Urogenital Diseases; D009059:Mouth Diseases / Q000382:microbiology; D018720:Prevotella / Q000302:isolation & purification / Q000378:metabolism*; D015203:Reproducibility of Results
D005353:Fibronectins; D007797:Laminin