Determination of PNU 157706, a new dual inhibitor of 5alpha-reductase, in rat plasma by high-performance liquid chromatography with ultraviolet detection.
A HPLC procedure was developed and validated for determining nanogram per milliliter concentrations of the dual 5alpha-reductase inhibitor PNU 157706 in rat plasma. The compound was extracted from plasma with diethyl ether followed by purification using a CN cartridge. The chromatographic separation was performed with a C18 column using a water-acetonitrile-methanol mixture as eluent. UV detection at 210 nm was used for the quantification of the compound over the concentration range 5-500 ng/ml plasma. The method has a lower limit of quantification of 5 ng/ml and good precision and accuracy. This method has performed well during analysis of several toxicokinetic and pharmacokinetic studies in the rat.
Basileo
G
G
Drug Metabolism Research, Pharmacia & Upjohn, Milan, Italy.
Breda
M
M
James
C A
CA
D000736:Androstenes / Q000097:blood*; D000818:Animals; D042944:Cholestenone 5 alpha-Reductase; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000097:blood*; D008297:Male; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000736:Androstenes; D004791:Enzyme Inhibitors; C112526:PNU 157706; D010088:Oxidoreductases; D042944:Cholestenone 5 alpha-Reductase