The BCR-ABL oncoprotein potentially interacts with the xeroderma pigmentosum group B protein.
The previously uncharacterized CDC24 homology domain of BCR, which is missing in the P185 BCR-ABL oncogene of Philadelphia chromosome (Ph1)-positive acute lymphocytic leukemia but is retained in P210 BCR-ABL of chronic myelogeneous leukemia, was found to bind to the xeroderma pigmentosum group B protein (XPB). The binding appeared to be required for XPB to be tyrosine-phosphorylated by BCR-ABL. The interaction not only reduced both the ATPase and the helicase activities of XPB purified in the baculovirus system but also impaired XPB-mediated cross-complementation of the repair deficiency in rodent UV-sensitive mutants of group 3. The persistent dysfunction of XPB may in part underlie genomic instability in blastic crisis.
Takeda
N
N
Department of Genetics, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan.
Shibuya
M
M
Maru
Y
Y
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001752:Blast Crisis / Q000209:etiology*; D016466:CHO Cells; D018797:Cell Cycle Proteins / Q000378:metabolism*; D006224:Cricetinae; D004265:DNA Helicases / Q000032:analysis; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics*; D011485:Protein Binding; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D014466:Ultraviolet Rays
C073594:CDC24 protein, S cerevisiae; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D020662:Guanine Nucleotide Exchange Factors; D011518:Proto-Oncogene Proteins; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; C072022:XPBC-ERCC-3 protein; D016044:Fusion Proteins, bcr-abl; D000251:Adenosine Triphosphatases; D004265:DNA Helicases