The use of pulsed field gel electrophoresis to investigate the epidemiology of Staphylococcus aureus infection in commercial broiler flocks.
Pulsed field gel electrophoresis (PFGE) analysis of SmaI restriction patterns was used to type 109 isolates of Staphylococcus aureus collected from broiler farms and hatcheries in Northern Ireland. Forty-seven isolates from clinical conditions in broilers and 62 strains from hatcheries, were examined. The PFGE patterns demonstrated a similarity between 85% of strains from clinical sources and 71% of the hatchery isolates. The association of disease with the predominant strain type and presence of these same strains in the hatchery, indicates that the hatchery is a potential source of the infection for clinical broiler disease.
McCullagh
J J
JJ
Department of Agriculture for Northern Ireland, Stormont, Belfast, N. Ireland. j.mccullagh@queens-belfast.ac.uk
McNamee
P T
PT
Smyth
J A
JA
Ball
H J
HJ
D000818:Animals; D001842:Bone and Bones / Q000382:microbiology; D002645:Chickens; D003129:Comb and Wattles / Q000382:microbiology; D004269:DNA, Bacterial / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D007494:Ireland / Q000453:epidemiology; D007596:Joints / Q000382:microbiology; D008099:Liver / Q000382:microbiology; D011201:Poultry Diseases / Q000175:diagnosis / Q000453:epidemiology*; D015183:Restriction Mapping; D013203:Staphylococcal Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D013211:Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial