Progress curves--a mean for functional classification of cellulases.
The chain-end preference and processivity of the cellulases 1,4-beta-D-glucan-cellobiohydrolase I (CBH I) and 1,4-beta-D-glucan-cellobiohydrolase II (CBH II) from Trichoderma reesei and 1,4-beta-D-glucan-cellobiohydrolase 50 (CBH 50) and 1,4-beta-D-glucan-cellobiohydrolase 58 (CBH 58) from Phanerochaete chrysosporium were studied by comparing experimental degradation data on reducing end-labelled bacterial microcrystalline cellulose with computer simulations of different models. Our results with T. reesei and P. chrysosporium cellulases show that there is a common pattern of hydrolysis for CBH-I-type enzymes and another clearly distinguishable pattern for CBH-II-type enzymes, especially in the initial part of the progress curve.
Nutt
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A
Department of Biochemistry, University of Uppsala, Sweden. Anu.Nutt@biokem.uu.se
Sild
V
V
Pettersson
G
G
Johansson
G
G
D002480:Cellulase / Q000145:classification / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D043366:Cellulose 1,4-beta-Cellobiosidase; D006868:Hydrolysis; D007527:Isoenzymes / Q000145:classification / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology; D014242:Trichoderma / Q000201:enzymology
D007527:Isoenzymes; D002482:Cellulose; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; C109691:microcrystalline cellulose