Brain-derived neurotrophic factor does not prevent ionizing radiation-induced apoptosis in the developing rat brain.
In an attempt to learn whether brain-derived neurotrophic factor (BDNF) is able to protect neural cells from ionizing radiation-induced apoptosis in the developing brain, BDNF-transfected (F3N-BDNF) and mock-transfected (F3A MT) fibroblasts were grafted into the right germinal periventricular zone of rats at postnatal day 1 (P1). The rats were later irradiated at P3 with a single dose (2 Gy whole body irradiation) of gamma rays. Age-matched non-irradiated and non-grafted irradiated rats at P3 were used as controls. No differences in the number of apoptotic cells were observed between the ipsilateral and contralateral side of either F3N-BDNF- or F3A-MT-grafted irradiated rats Furthermore, counts of apoptotic cells were similar in non-grafted irradiated and grafted irradiated rats. These results show that BDNF has no capacity to prevent ionizing radiation-induced apoptosis in the developing rat brain.
Ferrer
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I
Unitat de Neuropatologia, Servei d'Anatomia Patologica, Hospital Princeps d'Espanya, Hospitalet de Llobregat, Spain.
Goutan
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E
Martí
E
E
Arenas
E
E
D000818:Animals; D000831:Animals, Newborn / Q000502:physiology; D017209:Apoptosis / Q000187:drug effects*; D001921:Brain / Q000187:drug effects / Q000473:pathology* / Q000528:radiation effects*; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D002552:Cerebral Ventricles / Q000601:surgery; D005347:Fibroblasts / Q000637:transplantation; D051381:Rats; D011916:Rats, Inbred F344; D014162:Transfection; D014916:Whole-Body Irradiation
D019208:Brain-Derived Neurotrophic Factor