title: NO release from NO donors and nitrovasodilators: comparisons between oxyhemoglobin and potentiometric assays.
abstract: Unraveling the biology, pharmacology, and toxicology of NO depends on accurate NO assays, two of the more common being the oxyHb (oxyhemoglobin) assay and potentiometric detection using a Clark-type NO-selective electrode. Comparison of the specificity and sensitivity of the oxyHb and potentiometric methods was carried out using a broad series of nitrovasodilators, including organic nitrates, nitrites, thionitrates, nitrosothiols, and diazenium diolates. Only with the more labile diazenium diolates was a linear relationship observed between the rates of NO release measured potentiometrically and the rate of oxyHb oxidation from the oxyHb assay. The nonlinear plots indicate that N,O-species other than NO itself are capable of oxidizing oxyHb.
authors.affiliation: Department of Chemistry, Queen's University, Kingston, Ontario K7L 3N6, Canada.
authors.forename: J D
authors.initials: JD
authors.lastname: Artz
authors.forename: G R
authors.initials: GR
authors.lastname: Thatcher
mesh_terms: D002138:Calibration; D003124:Colorimetry; D006868:Hydrolysis; D007700:Kinetics; D009569:Nitric Oxide / Q000032:analysis*; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000737:chemistry*; D011199:Potentiometry; D014665:Vasodilator Agents / Q000737:chemistry*
chemical_list: D010108:Oxyhemoglobins; D014665:Vasodilator Agents; D009569:Nitric Oxide