Elevation of cytoplasmic cytochrome c in radiation-induced apoptosis.
The signal transduction pathway involved in radiation-induced apoptosis remains unclear, especially as regards the site at which the primary effect of radiation occurs. In this study, we demonstrate that cytochrome c may be released from mitochondria into the cytosol after irradiation, but that direct irradiation of isolated mitochondria induces no elevation of cytochrome c release. These observations suggest that cytochrome c and mitochondria may be involved in the radiation-induced apoptotic pathway, but mitochondria might not be a target of radiation, and that a signal transduction pathway from an unknown target might exist upstream of mitochondria during radiation-induced apoptosis.
Kurihara
M
M
Department of Otolaryngology, Yokohama City University School of Medicine.
Kurihara
H
H
Tsukuda
M
M
D017209:Apoptosis / Q000502:physiology / Q000528:radiation effects*; D003574:Cytochrome c Group / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D053938:DNA Fragmentation / Q000528:radiation effects; D018922:HL-60 Cells; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000528:radiation effects; D015398:Signal Transduction
D003574:Cytochrome c Group