Submicromolar paclitaxel induces apoptosis in human gastric cancer cells at early G1 phase.
Paclitaxel induced apoptosis has been reported in many cancer cell lines, but the relationship between G2/M phase arrest and apoptosis is not clear. We have reported that low dose (10 nM) paclitaxel induced apoptosis in gastric cancer cells without G2/M phase arrest. In this study, SC-M1 gastric cancer cells were synchronized at early G1, late G1, S and G2/M phases by staurosporine, mimosine, hydroxyurea and berberine, respectively. We have found that paclitaxel could not induce apoptosis of gastric cancer cells in late G1, S and G2/M phases; however, paclitaxel induced apoptosis in the early G1 phase. Our results suggest that cells arrested at G2/M phase by paclitaxel eventually entered the early G1 phase then proceeded to apoptosis.
Lin
H L
HL
Department of Medical Research, Veterans General Hospital-Taipei, Taiwan.
Chang
Y F
YF
Liu
T Y
TY
Wu
C W
CW
Chi
C W
CW
D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage*; D017209:Apoptosis; D001599:Berberine / Q000494:pharmacology; D019927:Cyclin E / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D016195:G2 Phase / Q000187:drug effects; D006801:Humans; D008898:Mimosine / Q000494:pharmacology; D017239:Paclitaxel / Q000008:administration & dosage*; D019311:Staurosporine / Q000494:pharmacology; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000972:Antineoplastic Agents, Phytogenic; D019927:Cyclin E; D001599:Berberine; D008898:Mimosine; D019311:Staurosporine; D017239:Paclitaxel