title: Evidence for loss and recovery of chick brainstem auditory neurons during gentamicin-induced cochlear damage and regeneration.
abstract: It is well documented that damage to the chick cochlea caused by acoustic overstimulation or ototoxic drugs is reversible. Second-order auditory neurons in nucleus magnocellularis (NM) are sensitive to changes in input from the cochlea. However, few experiments studying changes in NM during cochlear hair cell loss and regeneration have been reported. Chicks were given a single systemic dose of gentamicin, which results in maximal hair cell loss in the base of the cochlea after 5 days. Many new hair cells are present by 9 days. These new hair cells are mature but not completely recovered in organization by 70 days. We counted neurons in Nissl-stained sections of the brainstem within specific tonotopic regions of NM, comparing absolute cell number between gentamicin- and saline-treated animals at both short and long survival times. Our data suggest that neuronal number in rostral NM parallels hair cell number in the base of the cochlea. That is, after a single dose of gentamicin, we see a loss of both cochlear hair cells and NM neurons early, followed by a recovery of both cochlear hair cells and NM neurons later. These results suggest that neurons, like cochlear hair cells, can recover following gentamicin-induced damage.
authors.affiliation: Department of Otolaryngology and the Smith Mental Retardation Research Center, University of Kansas Medical Center, Kansas City, 66160-7380, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Park
authors.forename: D A
authors.initials: DA
authors.lastname: Girod
authors.forename: D
authors.initials: D
authors.lastname: Durham
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001306:Auditory Pathways / Q000473:pathology / Q000503:physiopathology*; D001933:Brain Stem / Q000473:pathology / Q000503:physiopathology*; D002645:Chickens; D003051:Cochlea / Q000187:drug effects / Q000473:pathology; D015834:Cochlear Diseases / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology*; D005839:Gentamicins; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D009474:Neurons / Q000502:physiology*; D012038:Regeneration / Q000502:physiology*; D013377:Substantia Innominata / Q000187:drug effects / Q000473:pathology
chemical_list: D005839:Gentamicins