Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation.
Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by gradient centrifugation for use in assisted reproductive procedures. Efficacy was assessed in terms of percentages of sperm recovery, sperm vitality and motility, normal sperm morphology and normal sperm chromatin condensation. No significant difference was found in the recovery of spermatozoa for men with both normal sperm counts and oligozoospermia, between PVP-coated and silane-coated particle solutions. Iodixanol had significantly lower sperm recovery compared to the other products. Sperm vitality, progressive motility, normal morphology and normal chromatin condensation did not differ significantly between any of the sperm isolation products.
Claassens
O E
OE
Queensland Fertility Group, Brisbane, Australia.
Menkveld
R
R
Harrison
K L
KL
D000177:Acrosome / Q000648:ultrastructure; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D002499:Centrifugation, Density Gradient / Q000379:methods*; D002843:Chromatin / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008863:Microspheres; D009845:Oligospermia / Q000473:pathology; D011205:Povidone; D012821:Silanes; D012822:Silicon Dioxide; D012996:Solutions; D013081:Sperm Motility; D013094:Spermatozoa / Q000166:cytology* / Q000648:ultrastructure; D014283:Triiodobenzoic Acids
D002843:Chromatin; D012821:Silanes; D012996:Solutions; D014283:Triiodobenzoic Acids; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone; C044834:iodixanol