title: Evaluation of the diagnostic performance of the Boehringer Mannheim CEDIA LSD assay.
abstract: The precision and the diagnostic performance of the Boehringer Mannheim CEDIA DAU LSD assay was evaluated. The assay was performed in the semi-quantitative mode on a Hitachi 917 analyzer. Within-run coefficients of variation (CVs) of the semiquantitative values for 0.25 and 1.0 ng/mL were 11.2 and 6.2%, respectively. Day-to-day CVs for the same concentrations were 12.6 and 8.6%. We analyzed 318 urine samples by CEDIA, DPC Coat-A-Count RIA and Behring EMIT II. Confirmation was performed by GC-MS, after extraction on Bond Elut Certify columns. Two hundred sixty-three samples were negative by all methods. Twenty-five samples were positive by all immunoassays, 19 of which were confirmed by gas chromatography-mass spectrometry (GC-MS). One sample was falsely negative by CEDIA. Three samples were positive by EMIT and CEDIA, but negative by RIA and GC-MS. Twenty-six samples were positive by EMIT alone, but they were not confirmed by GC-MS. The LSD CEDIA assay seems to be less specific than DPC RIA but more specific than the EMIT LSD assay.
authors.affiliation: Laboratory of Clinical Biology-Toxicology, University Hospital, Gent, Belgium.
authors.forename: A G
authors.initials: AG
authors.lastname: Verstraete
authors.forename: S
authors.initials: S
authors.lastname: Steyaert
mesh_terms: D017248:Enzyme Multiplied Immunoassay Technique; D008401:Gas Chromatography-Mass Spectrometry; D006213:Hallucinogens / Q000652:urine*; D006801:Humans; D008238:Lysergic Acid Diethylamide / Q000032:analysis / Q000652:urine*; D011863:Radioimmunoassay; D012680:Sensitivity and Specificity; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D006213:Hallucinogens; D008238:Lysergic Acid Diethylamide