title: Polymorphisms of twelve short tandem repeat loci in a Taiwanese population and their application in parentage testing.
abstract: With the advancement of techniques in molecular biology, rapid, sensitive, and reliable methods of DNA typing for parentage testing have become available. In this study, we evaluated the usefulness of multiplex polymerase chain reaction (PCR) with 12 unlinked short tandem repeat (STR) loci for paternity testing in Taiwan. The genetic informativeness of this test was then compared with that of conventional human leukocyte antigen (HLA) analysis in 167 parentage studies. The 12 STR loci alone provided a cumulative power of exclusion of up to 0.9998. Paternity was excluded in 59 (35.3%) cases, including 40 of 112 paternity trios and 19 of 55 paternity duos. In the 40 trios in which paternity was excluded, a mean of 6 (range, 3-9) incompatible STR markers were in the 19 duos in which paternity was excluded, a mean of 4 (range, 1-8) incompatible STR markers were noted. In the 72 trios in which the alleged paternity could not be excluded, the mean probabilities of paternity (PP) were 90.6863% with HLA testing alone, 99.9847% with STR analysis alone, and 99.9972% with combined HLA and STR analysis. In the 36 duos in which the alleged paternity could not be excluded, the mean PPs were 81.4768% with HLA testing alone, 99.6124% with STR analysis alone, and 99.9145% with combined HLA and STR analysis. These results suggest that STR analysis is very powerful when used alone for paternity trio testing and when combined with conventional serologic HLA typing for duo parentage testing in the Taiwan population.
authors.affiliation: Section of Transfusion Medicine, Veterans General Hospital-Taipei, School of Medicine, National Yang-Ming University, Taiwan.
authors.forename: C H
authors.initials: CH
authors.lastname: Tzeng
authors.forename: J Y
authors.initials: JY
authors.lastname: Lyou
authors.forename: Y R
authors.initials: YR
authors.lastname: Chen
authors.forename: H Y
authors.initials: HY
authors.lastname: Hu
authors.forename: J S
authors.initials: JS
authors.lastname: Lin
authors.forename: C H
authors.initials: CH
authors.lastname: Yung
authors.forename: K B
authors.initials: KB
authors.lastname: Choo
authors.forename: S Y
authors.initials: SY
authors.lastname: Wang
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D002874:Chromosome Mapping; D005260:Female; D006650:Histocompatibility Testing; D006801:Humans; D008297:Male; D010334:Paternity; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences