Tyrosine phosphorylation of syndecan-1 and -4 cytoplasmic domains in adherent B82 fibroblasts.
The syndecans, a family of cell surface proteoglycans, have highly conserved cytoplasmic domains that bind proteins containing PDZ domains and co-localize with the actin cytoskeleton. The syndecan cytoplasmic domains contain four conserved tyrosine residues, two of which are located within favorable sequences for phosphorylation. Endogenous tyrosine phosphorylation of syndecans-1 and -4 is detected in adherent B82 fibroblasts. Approximately 1.5% of total syndecan is endogenously phosphorylated, while most, if not all, cell surface syndecan is phosphorylated following treatment with the tyrosine phosphatase inhibitor pervanadate. Syndecan phosphorylation is also detected in Raji-S1 and NMuMG cells, but only following treatment with vanadate or pervanadate, suggesting that endogenous phosphorylation is maintained in an "off" state in these cells. Endogenous syndecan phosphorylation in B82 cells is rapidly blocked by genistein (IC50 < 10 microM) confirming the presence of a constitutively active kinase and a corresponding tyrosine phosphatase. Phosphorylation is also inhibited by herbimycin A (IC50 < 1.0 microM) and staurosporine (IC50 < 1.0 nM), suggesting a role for Src family kinases in regulating syndecan phosphorylation. Together, these data suggest an important role for tyrosine phosphorylation of the syndecan cytoplasmic domains in regulating downstream signaling events in response to cell adhesion and/or growth factor activity.
Ott
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Program in Cellular and Molecular Biology and Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, Wisconsin 53706, USA.
Rapraeger
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D000595:Amino Acid Sequence; D016227:Benzoquinones; D002448:Cell Adhesion; D016764:Cell Polarity; D003593:Cytoplasm; D005347:Fibroblasts / Q000378:metabolism; D019833:Genistein / Q000494:pharmacology; D047029:Lactams, Macrocyclic; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010766:Phosphorylation; D047428:Protein Kinase Inhibitors; D011509:Proteoglycans / Q000378:metabolism*; D011809:Quinones / Q000494:pharmacology; D017828:Rifabutin / Q000031:analogs & derivatives; D019311:Staurosporine / Q000494:pharmacology; D053669:Syndecan-2; D053671:Syndecan-4; D053667:Syndecans; D014443:Tyrosine / Q000378:metabolism; D019061:src-Family Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D016227:Benzoquinones; D047029:Lactams, Macrocyclic; D008562:Membrane Glycoproteins; D047428:Protein Kinase Inhibitors; D011509:Proteoglycans; D011809:Quinones; D053671:Syndecan-4; D053667:Syndecans; D053669:Syndecan-2; D017828:Rifabutin; D014443:Tyrosine; C020754:herbimycin; D019833:Genistein; D019061:src-Family Kinases; D019311:Staurosporine