title: The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids.
abstract: Mi-1, a Lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. Complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode Meloidogyne incognita and to an unrelated pathogen, the potato aphid Macrosiphum euphorbiae. This resistance was stable. The Mi-1 gene encodes a protein sharing structural features with the nucleotide-binding site leucine-rich repeat-containing type of plant resistance genes.
authors.affiliation: Keygene N.V., Agro Business Park, Wageningen, The Netherlands. keygene@euronet.nl
authors.forename: P
authors.initials: P
authors.lastname: Vos
authors.forename: G
authors.initials: G
authors.lastname: Simons
authors.forename: T
authors.initials: T
authors.lastname: Jesse
authors.forename: J
authors.initials: J
authors.lastname: Wijbrandi
authors.forename: L
authors.initials: L
authors.lastname: Heinen
authors.forename: R
authors.initials: R
authors.lastname: Hogers
authors.forename: A
authors.initials: A
authors.lastname: Frijters
authors.forename: J
authors.initials: J
authors.lastname: Groenendijk
authors.forename: P
authors.initials: P
authors.lastname: Diergaarde
authors.forename: M
authors.initials: M
authors.lastname: Reijans
authors.forename: J
authors.initials: J
authors.lastname: Fierens-Onstenk
authors.forename: M
authors.initials: M
authors.lastname: de Both
authors.forename: J
authors.initials: J
authors.lastname: Peleman
authors.forename: T
authors.initials: T
authors.lastname: Liharska
authors.forename: J
authors.initials: J
authors.lastname: Hontelez
authors.forename: M
authors.initials: M
authors.lastname: Zabeau
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D003001:Cloning, Molecular; D003360:Cosmids; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000235:genetics* / Q000469:parasitology; D008969:Molecular Sequence Data; D009348:Nematoda; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011198:Solanum tuberosum / Q000469:parasitology*
chemical_list: C117349:MI-1 protein, Lycopersicon esculentum; D010940:Plant Proteins