Envelope and long terminal repeat sequences of an infectious murine leukemia virus from a human SCLC cell line: implications for gene transfer.
Development of methods for gene transfer into specific cell types or tissues is important for experimental research as well as clinical therapeutical approaches. We report here the cloning and characterization of the envelope (env) gene and the U3 region of a retrovirus from an infected human Small Cell Lung Cancer (SCLC) cell line. The replication of this murine retrovirus is also fully supported by other lung cancer cell lines of different histological origin. We present evidence that a long terminal repeat (LTR)-beta-galactosidase (beta-Gal) reporter construct performed as well as an analogous cytomegalovirus (CMV) promoter beta-Gal construct in the human lung epithelial cell line A549 and in the human larynx carcinoma cell line HEp2.
Antoine
M
M
Ruhr-Universität Bochum, Medizinische Fakultät, Institut für Hygiene und Mikrobiologie, Abteilung für Med. Mikrobiol. und Virologie, Gebäude, Germany.
Wegmann
B
B
Kiefer
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D015139:Blotting, Southern; D018288:Carcinoma, Small Cell; D003001:Cloning, Molecular; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015752:Genes, env / Q000235:genetics*; D006801:Humans; D009052:Leukemia Virus, Murine / Q000235:genetics* / Q000502:physiology; D008175:Lung Neoplasms; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D020079:Terminal Repeat Sequences / Q000235:genetics*; D014407:Tumor Cells, Cultured; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics; D014779:Virus Replication; D001616:beta-Galactosidase / Q000378:metabolism
D014759:Viral Envelope Proteins; D001616:beta-Galactosidase