title: Modulation of the neurotransmitter release in rat cerebellar neurons by GRO beta.
abstract: We report here that, in cultured cerebellar granule cells, the CXC chemokine GRObeta stimulates the signaling pathway of the extracellular signal-regulated kinases, and enhances both evoked and spontaneous postsynaptic currents in patch clamped Purkinje neurons from rat cerebellar slices. The GRObeta-induced enhancement of the excitatory post-synaptic currents evoked by stimulating the parallel fibres is blocked by the inhibitor of the extracellular signal-regulated kinases pathway PD98059, which also reduces both basal frequency of spontaneous post-synaptic currents and mean amplitude of evoked excitatory post-synaptic currents. Our results suggest that GRObeta modulates neurotransmitter release in the cerebellum through the activation of the extracellular signal-regulated kinases pathway.
authors.affiliation: Dipartimento di Medicina Sperimentale e Patologia Università di Roma La Sapienza, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Ragozzino
authors.forename: A
authors.initials: A
authors.lastname: Giovannelli
authors.forename: A M
authors.initials: AM
authors.lastname: Mileo
authors.forename: C
authors.initials: C
authors.lastname: Limatola
authors.forename: A
authors.initials: A
authors.lastname: Santoni
authors.forename: F
authors.initials: F
authors.lastname: Eusebi
mesh_terms: D000818:Animals; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D002478:Cells, Cultured; D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology; D006131:Growth Inhibitors / Q000378:metabolism*; D006133:Growth Substances / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009924:Organ Culture Techniques; D018408:Patch-Clamp Techniques; D011689:Purkinje Cells / Q000166:cytology / Q000201:enzymology*; D051381:Rats; D015398:Signal Transduction / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology; D013779:Tetrodotoxin / Q000494:pharmacology
chemical_list: D054360:Chemokine CXCL1; D019743:Chemokines, CXC; D002630:Chemotactic Factors; C513297:Cxcl1 protein, rat; D004791:Enzyme Inhibitors; D005419:Flavonoids; D006131:Growth Inhibitors; D006133:Growth Substances; D036341:Intercellular Signaling Peptides and Proteins; D018377:Neurotransmitter Agents; D013779:Tetrodotoxin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one