In vitro and in vivo fibrochondrocyte growth behavior in fibrin gel: an immunohistochemical study in the rabbit.
This study investigated matrix formation of rabbit meniscal fibrochondrocytes synthesized in vitro and the viability of fibrochondrocytes encapsulated in fibrin gel after transplantation into rabbit meniscal lesion sites in vivo. The matrix of newly formed meniscal fibrochondrocytes encapsulated in fibrin gel was evaluated using immunohistochemical methods. The meniscal cells in the three-dimensional encapsulating fibrin gel proliferated well for a 2-month period. Examination of the in vivo transplantation confirmed that the newly formed cartilage produced S-100 protein. These observations suggest that meniscal fibrochondrocytes encapsulated in fibrin gel are suitable for cell division and matrix production, and the use of fibrin gel as a scaffolding delivery substance may be beneficial for the transplantation of viable meniscal fibrochondrocytes in resurfacing meniscoplasty.
Isoda
K
K
Department of Orthopaedic Surgery, Inagi City Hospital, Japan.
Saito
S
S
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects* / Q000473:pathology / Q000637:transplantation*; D003470:Culture Media; D004195:Disease Models, Animal; D005260:Female; D005337:Fibrin / Q000494:pharmacology / Q000502:physiology*; D005782:Gels; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008592:Menisci, Tibial / Q000473:pathology* / Q000601:surgery*; D010784:Photomicrography; D011817:Rabbits; D009418:S100 Proteins / Q000032:analysis
D003470:Culture Media; D005782:Gels; D009418:S100 Proteins; D005337:Fibrin