title: Liquid chromatographic separation of some PTH-amino acids.
abstract: Liquid chromatographic studies on the separation of ten PTH-amino acids were carried out using normal phase untreated silica gel plates, C-18 RP precoated plates and RP-HPLC. Resolution of a complex mixture of PTH-amino acids was achieved using all the three types. Certain new successful solvent systems have been worked out in each case. HPLC was carried out with Lichrosphere 100 RP-18 (5 microns) column. Acetonitrile and sodium acetate buffer of pH 4.0 was used for reversed phase chromatography while for normal phase TLC combinations of chloroform-acetonitrile and chloroform-tetrahydrofuran were applied.
authors.affiliation: Department of Chemistry, University of Roorkee, India.
authors.forename: R
authors.initials: R
authors.lastname: Bhushan
authors.forename: R
authors.initials: R
authors.lastname: Agarwal
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000596:Amino Acids; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin