cDNA cloning and expression analysis of the human UDPglucose dehydrogenase.
A cDNA clone encoding the human UDPglucose dehydrogenase was isolated from a liver cDNA library. The cDNA is 2,355 bp in length with an open reading frame which is capable of encoding a protein of 494 residues. The predicted primary sequence of the gene product is in good agreement with that of the bovine enzyme determined previously found by means of protein sequencing. Two major transcripts of the UDPglucose dehydrogenase gene with sizes of 2.8 and 2.35 kb, respectively, were observed by Northern analysis. The gene was found to be expressed in a variety of tissues with the highest level in liver, consistent with the physiological function of the enzyme in excretion of endo- and xenobiotics compounds.
Peng
H L
HL
Institute of Biological Science and Technology, National Chiao-Tung University, Hsin Chu, Taiwan, R.O.C.
Lou
M D
MD
Chang
M L
ML
Chang
H Y
HY
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004331:Drosophila melanogaster / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D015723:Gene Library; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013025:Glycine max / Q000201:enzymology; D014158:Transcription, Genetic; D014533:Uridine Diphosphate Glucose Dehydrogenase / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*
D018076:DNA, Complementary; D011994:Recombinant Proteins; D014533:Uridine Diphosphate Glucose Dehydrogenase