title: Compensatory changes in GroEL/Gp31 affinity as a mechanism for allele-specific genetic interaction.
abstract: Previous work has shown that the GroEL-GroES interaction is primarily mediated by the GroES mobile loop. In bacteriophage T4 infection, GroES is substituted by the gene 31-encoded cochaperonin, Gp31. Using a genetic selection scheme, we have identified a new set of mutations in gene 31 that affect interaction with GroEL; all mutations result in changes in the mobile loop of Gp31. Biochemical analyses reveal that the mobile loop mutations alter the affinity between Gp31 and GroEL, most likely by modulating the stability of the GroEL-bound hairpin conformation of the mobile loop. Surprisingly, mutations in groEL that display allele-specific interactions with mutations in gene 31 alter residues in the GroEL intermediate domain, distantly located from the mobile loop binding site. The observed patterns of genetic and biochemical interaction between GroES or Gp31 and GroEL point to a mechanism of genetic allele specificity based on compensatory changes in affinity of the protein-protein interaction. Mutations studied in this work indirectly alter affinity by modulating a folding transition in the Gp31 mobile loop or by modulating a hinged conformational change in GroEL.
authors.affiliation: Département de Biochimie Médicale, University of Geneva, 1 rue Michel-Servet, 1211 Geneva, Switzerland. Alexandra.Richardson@medecine.unige.ch
authors.forename: A
authors.initials: A
authors.lastname: Richardson
authors.forename: S M
authors.initials: SM
authors.lastname: van der Vies
authors.forename: F
authors.initials: F
authors.lastname: Keppel
authors.forename: A
authors.initials: A
authors.lastname: Taher
authors.forename: S J
authors.initials: SJ
authors.lastname: Landry
authors.forename: C
authors.initials: C
authors.lastname: Georgopoulos
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D017122:Bacteriophage T4 / Q000235:genetics; D018834:Chaperonin 60 / Q000737:chemistry / Q000378:metabolism*; D018833:Chaperonins / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding; D013050:Spectrometry, Fluorescence; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018834:Chaperonin 60; D014764:Viral Proteins; C063473:gene 31 protein, Enterobacteria phage T4; D002950:Citrate (si)-Synthase; D018833:Chaperonins