A mouse model for beta cell-specific ablation of target gene(s) using the Cre-loxP system.
The rat insulin promoter (RIP) has been used to drive the expression of Cre recombinase (Cre) specifically in beta cells. Transient transfection was performed in the mouse insulinoma cell line, NIT-1, and control cell lines. RT-PCR was performed using total RNA from pancreas and other tissues of RIP-Cre transgenic mice. In addition, the efficiency and specificity of RIP were further analyzed by cross breeding the RIP-Cre transgenic mice with reporter mice bearing a beta-actin-loxP-CAT-loxP-lacZ transgene. In these mice, lacZ is expressed only after excision of the floxed-CAT gene by Cre-mediated recombination. Here, we present the data for beta cell-specific expression of lacZ in bigenic mice, as proof of concept in a mouse model for targeting beta cell-specific gene(s). The RIP-Cre transgenic mice will be used as a potential tool for targeting the excision of beta cell-specific gene(s) to study their role in islet cell physiology.
Ray
M K
MK
Department of Surgery, Baylor College of Medicine, Houston, Texas 77030, USA. mray@bcm.tmc.edu
Fagan
S P
SP
Moldovan
S
S
DeMayo
F J
FJ
Brunicardi
F C
FC
D000818:Animals; D002460:Cell Line; D003433:Crosses, Genetic; D005786:Gene Expression Regulation; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter; D007328:Insulin / Q000235:genetics; D007340:Insulinoma; D019426:Integrases / Q000235:genetics*; D007515:Islets of Langerhans / Q000378:metabolism* / Q000502:physiology; D007763:Lac Operon; D051379:Mice; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D019076:Transgenes; D014764:Viral Proteins; D001616:beta-Galactosidase / Q000235:genetics
D007328:Insulin; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase