Isolation of an Arabidopsis thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity.
The yeast Abc1 protein acts as a chaperone-like protein essential for the proper conformation and efficient functioning of the respiratory complex III. By functional complementation of a yeast abc1 mutant, we have identified an Arabidopsis thaliana cDNA that corresponds to a single copy gene and encodes a protein sharing 45% similarity with the yeast Abc1p protein. Cytochrome spectra and respiratory activity measurements have shown that the plant protein allows a partial restoration of the complex III activity. No major difference in the steady-state level of ABC1At mRNA was observed in various plant tissues, suggesting that ABC1At is constitutively expressed in A. thaliana. Phylogenetic analysis revealed that the Abc1At protein belongs to a large family of proteins composed of two eukaryotic and one prokaryotic subgroups differing by their degree of similarity and probably by their function.
Cardazzo
B
B
Centre de Génétique Moléculaire, Gif sur Yvette, France.
Hamel
P
P
Sakamoto
W
W
Wintz
H
H
Dujardin
G
G
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification*; D014450:Electron Transport Complex III / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
C099891:COQ8 protein, S cerevisiae; D018076:DNA, Complementary; D005656:Fungal Proteins; D010940:Plant Proteins; D029701:Saccharomyces cerevisiae Proteins; D014450:Electron Transport Complex III