Altered biologic activities of commercial polychlorinated biphenyl mixtures after microbial reductive dechlorination.
The reductive dechlorination of polychlorinated biphenyls (PCBs) by anaerobic bacteria has recently been established as an important environmental fate of these compounds. This process removes chlorines directly from the biphenyl ring with replacement by hydrogen, resulting in a product mixture in which the average number of chlorines per biphenyl is reduced. In this study, dechlorination of commercial PCB mixtures (Aroclors 1242 and 1254) by microorganisms eluted from PCB-contaminated sediments of the River Raisin (Michigan) and Silver Lake (Massachusetts) caused a depletion in the proportion of highly chlorinated PCB congeners and an accumulation of lesser-chlorinated congeners. Dechlorination occurred primarily at the meta and, to a much lesser extent, para positions of biphenyl. The concentrations of the coplanar congeners including 3,3',4,4',5-pentachlorobiphenyl, the most potent dioxinlike congener, were significantly lowered by reductive dechlorination. Microbial reductive dechlorination of commercial PCB mixtures caused a substantial reduction in biologic activities in several instances. It significantly lowered or eliminated the inhibitory effects of Aroclors on fertilization of mouse gametes in vitro. Similarly, the dechlorinated product mixtures had substantially lower ethoxyresorufin-O-deethylase induction potencies and showed less ability to induce activating protein 1 transcription factor activity as compared to the unaltered Aroclors. In other assays the same dechlorinated product mixtures demonstrated biologic activities similar to the nondechlorinated Aroclors, including the ability of PCB mixtures to stimulate insulin secretion and cause neutrophil activation. The data presented here establish that the biologic activities of commercial PCB mixtures are altered by microbial reductive dechlorination and that an assessment of their toxic potential requires an array of tests that include the different mechanisms associated with PCBs.
Mousa
M A
MA
Department of Crop and Soil Sciences, Michigan State University, East Lansing 48824-1325, USA.
Ganey
P E
PE
Quensen
J F
JF
Madhukar
B V
BV
Chou
K
K
Giesy
J P
JP
Fischer
L J
LJ
Boyd
S A
SA
D000818:Animals; D001673:Biodegradation, Environmental; D002713:Chlorine / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism; D005260:Female; D005307:Fertilization in Vitro; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D008297:Male; D051379:Mice; D018375:Neutrophil Activation / Q000502:physiology; D010084:Oxidation-Reduction; D011078:Polychlorinated Biphenyls / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity*
D007328:Insulin; D002713:Chlorine; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1