title: Identification and functional analysis of a Kluyveromyces lactis homologue of the SPT4 gene of Saccharomyces cerevisiae.
abstract: Sequence analysis of a DNA fragment containing the KlCOX18 gene originating from chromosome II of the yeast Kluyveromyces lactis revealed the presence of an adjacent open reading frame (ORF) for a protein exhibiting 78.4% identity with the Saccharomyces cerevisiae Spt4p. Based on the identical length (102 aa) and the conservation of the zinc-finger motif found in Spt4p we named this ORF KlSPT4. When expressed in S. cerevisiae the KlSPT4 gene complemented all spt4 mutant phenotypes. It is proposed that KlSpt4p, like its S. cerevisiae counterpart is a protein involved in the establishment or maintenance of the chromatin structure that influences the expression of many yeast genes.
authors.affiliation: Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Mlynská dolina B2, 84215 Bratislava, Slovak Republic.
authors.forename: I
authors.initials: I
authors.lastname: Hikkel
authors.forename: Y
authors.initials: Y
authors.lastname: Gbelská
authors.forename: J
authors.initials: J
authors.lastname: Subík
mesh_terms: D000595:Amino Acid Sequence; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal / Q000235:genetics* / Q000502:physiology; D005816:Genetic Complementation Test; D007716:Kluyveromyces / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D035602:Transcriptional Elongation Factors
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D009687:Nuclear Proteins; C080991:SPT4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D035602:Transcriptional Elongation Factors