Human preembryo development on autologous endometrial coculture versus conventional medium.
OBJECTIVE
To evaluate the effect of autologous endometrial coculture versus conventional medium on preembryo development.


METHODS
Controlled systematic clinical study.


METHODS
University-based IVF center.


METHODS
Women with a history of failed IVF-ET with poor preembryo quality.


METHODS
Patients underwent a luteal phase endometrial biopsy. The tissue then was digested enzymatically, and the stromal and glandular cells were separated by differential sedimentation rates. These cells were cultured to confluence, released, and then cryopreserved until the patient's IVF-ET cycle. All normally fertilized oocytes then were allocated systematically to growth on autologous endometrial coculture or conventional medium until transfer on day 3.


METHODS
Preembryo blastomere numbers and cytoplasmic fragmentation rates were measured.


RESULTS
Forty-two women underwent 44 cycles of IVF-ET. In the morning on day 3, the mean (+/-SD) number of blastomeres and cytoplasmic fragments per preembryo on coculture compared with conventional medium was 5.9+/-1.5 versus 5.5+/-1.4 and 21%+/-13% versus 24%+/-11. At transfer the mean (+/-SD) number of blastomeres per preembryo on coculture was 7.4+/-1.3 versus 6.7+/-1.9 on conventional medium.


CONCLUSIONS
There was a significant improvement in the mean (+/-SD) number of blastomeres per preembryo and decrease in the fragmentation rate for preembryos on autologous endometrial coculture compared with noncocultured preembryos from the same patient.
Barmat
L I
LI
The Center For Reproductive Medicine and Infertility, The New York Hospital-Cornell Medical Center, New York, USA.
Liu
H C
HC
Spandorfer
S D
SD
Xu
K
K
Veeck
L
L
Damario
M A
MA
Rosenwaks
Z
Z
D000328:Adult; D001706:Biopsy; D001755:Blastocyst / Q000502:physiology*; D001757:Blastomeres / Q000502:physiology; D018920:Coculture Techniques; D003470:Culture Media; D004624:Embryo Transfer; D004717:Endometrium / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D008183:Luteal Phase; D018873:Pregnancy Rate; D014182:Transplantation, Autologous; D016896:Treatment Outcome
D003470:Culture Media