5'-Peptidyl substituents allow a tuning of the affinity of oligodeoxyribonucleotides for RNA.
The affinity of amide-linked 5'-aminoacyl and 5'-dipeptidyl DNA octamers for two RNA undecamers with 3'-overhangs was measured via UV melting analysis. A sequence-dependent increase in melting points was observed. At low ionic strength, two appended lysine residues elevate melting points more than two additional A:U base pairs.
Sarracino
D A
DA
Department of Chemistry, Tufts University, Medford, MA 02155, USA.
Steinberg
J A
JA
Vergo
M T
MT
Woodworth
G F
GF
Tetzlaff
C N
CN
Richert
C
C
D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D004151:Dipeptides; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA