title: Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens.
abstract: Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10,050 and 13,706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: M
authors.initials: M
authors.lastname: Degawa
authors.forename: E
authors.initials: E
authors.lastname: Kawata
authors.forename: J
authors.initials: J
authors.lastname: Hayashi
authors.forename: Y
authors.initials: Y
authors.lastname: Shoyama
mesh_terms: D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification*; D015153:Blotting, Western; D002188:Cannabis / Q000737:chemistry* / Q000276:immunology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D008970:Molecular Weight; D011058:Pollen / Q000737:chemistry* / Q000276:immunology; D012189:Retrospective Studies
chemical_list: D000485:Allergens; D007073:Immunoglobulin E