Oligoribonucleotides containing 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2'-O-methylguanosine: synthesis and base pairing properties.
7,8-Dihydroguanosine(8-oxoG) and 7,8-dihydro-2'-O-methylguanosine (8-oxoG-Me) were chemically synthesized. Oligoribonucleotides which contain 8-oxoG or 8-oxoG-Me were synthesized. Incorporation of nucleotides opposite 8-oxoG and 8-oxoG-Me with dNTP by MMLV reverse transcriptase during cDNA synthesis was characterized.
Kim
S K
SK
Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
Yokoyama
S
S
Takaku
H
H
Moon
B J
BJ
D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D018076:DNA, Complementary / Q000138:chemical synthesis; D003854:Deoxyribonucleotides; D006151:Guanosine / Q000031:analogs & derivatives*; D007202:Indicators and Reagents; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology; D051379:Mice; D015394:Molecular Structure; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012194:RNA-Directed DNA Polymerase
C116629:8-oxo-7,8-dihydro-2'-O-methylguanosine; D018076:DNA, Complementary; D003854:Deoxyribonucleotides; D007202:Indicators and Reagents; D009843:Oligoribonucleotides; D006151:Guanosine; C046215:8-hydroxyguanosine; D012194:RNA-Directed DNA Polymerase