title: Column liquid chromatography determination of vitamins A and E in powdered milk and local flour: a validation procedure.
abstract: A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application.
authors.affiliation: Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France.
authors.forename: M
authors.initials: M
authors.lastname: Ake
authors.forename: H
authors.initials: H
authors.lastname: Fabre
authors.forename: A K
authors.initials: AK
authors.lastname: Malan
authors.forename: B
authors.initials: B
authors.lastname: Mandrou
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007560:Cote d'Ivoire; D005433:Flour / Q000032:analysis*; D005519:Food Preservation; D008892:Milk / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins; D012997:Solvents; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
chemical_list: D012503:Saponins; D012997:Solvents; D014801:Vitamin A; D014810:Vitamin E