A highly conservative, cyclically permuted, non-homologous exchange among three unrelated DNA sequences in bleomycin-treated CHO cells.
Molecular analysis of a bleomycin-induced rearrangement of the aprt gene in CHO cells revealed that it consisted of a nearly perfect three-way exchange among non-homologous sequences, consistent with a mechanism involving cyclically permuted misjoining of the six ends of three double-strand breaks.
Povirk
L F
LF
Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298, USA. LPOVIRK@gems.vcu.edu
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics*; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000633:toxicity; D001483:Base Sequence; D001761:Bleomycin / Q000633:toxicity; D016466:CHO Cells / Q000187:drug effects; D002869:Chromosome Aberrations / Q000235:genetics*; D006224:Cricetinae; D004247:DNA / Q000187:drug effects* / Q000235:genetics; D015321:Gene Rearrangement; D008969:Molecular Sequence Data
D000964:Antimetabolites, Antineoplastic; D001761:Bleomycin; D004247:DNA; D000228:Adenine Phosphoribosyltransferase