Blood sampling site-dependent plasma concentrations and hepatotoxic parameters in serum after intraperitoneal administration of acetaminophen to rats.
Blood sampling site-dependent (the dorsal aorta, the jugular vein, and the retro-orbital sinus) plasma concentrations of acetaminophen and its glucuronide and sulfate conjugates, and acetaminophen-induced hepatotoxic parameters (such as ALT and SDH activity) in serum were evaluated after intraperitoneal administration of acetaminophen, 500 mg/kg body weight, to rats. The plasma concentrations and the resultant AUC0-12 h of acetaminophen, acetaminophen-glucuronide, and acetaminophen-sulfate were significantly higher when blood samples were collected from the retro-orbital sinus than those from the jugular vein. The serum ALT activity at 3 and 24 h after administration of acetaminophen were significantly higher when the blood samples were collected from the retro-orbital sinus than those from the dorsal aorta.
Yim
H K
HK
College of Pharmacy, Seoul National University, South Korea.
Kang
H M
HM
Lee
M G
MG
Kim
Y C
YC
D000082:Acetaminophen / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity*; D000410:Alanine Transaminase / Q000097:blood; D018712:Analgesics, Non-Narcotic / Q000097:blood* / Q000493:pharmacokinetics / Q000633:toxicity*; D000818:Animals; D019540:Area Under Curve; D001158:Arteries; D001800:Blood Specimen Collection / Q000379:methods; D005260:Female; D007274:Injections, Intraperitoneal; D007064:L-Iditol 2-Dehydrogenase / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008111:Liver Function Tests; D051381:Rats; D017207:Rats, Sprague-Dawley; D014680:Veins
D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D007064:L-Iditol 2-Dehydrogenase; D000410:Alanine Transaminase