Helicobacter pylori infection in immunized mice lacking major histocompatibility complex class I and class II functions.
The role of major histocompatibility complex (MHC) class I- and class II-restricted functions in Helicobacter pylori infection and immunity upon oral immunization was examined in vivo. Experimental challenge with H. pylori SS1 resulted in significantly greater (P </= 0.025) colonization of MHC class I and class II mutant mice than C57BL/6 wild-type mice. Oral immunization with H. pylori whole-cell lysates and cholera toxin adjuvant significantly reduced the magnitude of H. pylori infection in C57BL/6 wild-type (P = 0.0083) and MHC class I knockout mice (P = 0.0048), but it had no effect on the H. pylori infection level in MHC class II-deficient mice. Analysis of the anti-H. pylori antibody levels in serum showed a dominant serum immunoglobulin G1 (IgG1) response in immunized C57BL/6 wild-type and MHC class I mutant mice but no detectable serum IgG response in MHC class II knockout mice. Populations of T-cell-receptor (TCR) alphabeta+ CD4(+) CD54(+) cells localized to gastric tissue of immunized C57BL/6 wild-type and MHC class I knockout mice, but TCRalphabeta+ CD8(+) cells predominated in the gastric tissue of immunized MHC class II-deficient mice. These observations show that CD4(+) T cells engaged after mucosal immunization may be important for the generation of a protective anti-H. pylori immune response and that CD4(+) CD8(-) and CD4(-) CD8(+) T cells regulate the extent of H. pylori infection in vivo.
Pappo
J
J
Mucosal Immunology, Astra Research Center Boston, Inc., Cambridge, Massachusetts 02139, USA. jack.pappo@arcb.us.astra.com
Torrey
D
D
Castriotta
L
L
Savinainen
A
A
Kabok
Z
Z
Ibraghimov
A
A
D000284:Administration, Oral; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D005753:Gastric Mucosa / Q000276:immunology / Q000382:microbiology; D016481:Helicobacter Infections / Q000235:genetics* / Q000276:immunology* / Q000517:prevention & control; D016480:Helicobacter pylori / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000502:physiology; D000949:Histocompatibility Antigens Class II / Q000235:genetics* / Q000502:physiology; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II