title: Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.
abstract: Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.
authors.affiliation: Department of Medical Biochemistry, University of Göteborg, Gothenburg, Sweden.
authors.forename: M A
authors.initials: MA
authors.lastname: Axelsson
authors.forename: E M
authors.initials: EM
authors.lastname: Hansson
authors.forename: R
authors.initials: R
authors.lastname: Sikut
authors.forename: G C
authors.initials: GC
authors.lastname: Hansson
mesh_terms: D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D005740:Gases; D006858:Hydrofluoric Acid; D008567:Membranes, Artificial; D018396:Mucin-1 / Q000737:chemistry / Q000276:immunology; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins / Q000737:chemistry* / Q000276:immunology; D009757:Nylons; D009844:Oligosaccharides
chemical_list: D000911:Antibodies, Monoclonal; D005740:Gases; D008567:Membranes, Artificial; D018396:Mucin-1; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins; D009757:Nylons; D009844:Oligosaccharides; D006858:Hydrofluoric Acid