Production of antibody against saikosaponin a, an active component of bupleuri radix.
High titer rabbit polyclonal antibodies (pAbs) which show a specificity for saikosaponin a (SSA), have been generated. The immunogen used was a conjugate of SSA linked through its glucose moiety to bovine serum albumin by periodate oxidation method. The antibody titers obtained from two rabbits, inoculated with the immunogen, reached a plateau after the fourth and third booster injection, respectively. The specificity of the pAbs was determined by hapten inhibition assays using several SSA-like structures. SSA competitively inhibited the binding of the rabbit anti-SSA pAbs to SSA-ovalbumin on solid phase, a coated antigen on the well. The antibodies showed high specificity to SSA, exhibiting no significant cross-reactivity with any of SSA analogues tested.
Jung
D W
DW
College of Pharmacy, Chonnam National University, Kwangju, Korea.
Sung
C K
CK
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002240:Carbohydrate Sequence; D002417:Cattle; D003470:Culture Media; D006241:Haptens / Q000276:immunology; D007166:Immunosuppressive Agents / Q000276:immunology*; D008969:Molecular Sequence Data; D009828:Oleanolic Acid / Q000031:analogs & derivatives*; D010946:Plants, Medicinal / Q000737:chemistry*; D012502:Sapogenins / Q000276:immunology*; D012503:Saponins; D012710:Serum Albumin, Bovine
D000906:Antibodies; D003470:Culture Media; D006241:Haptens; D007166:Immunosuppressive Agents; D012502:Sapogenins; D012503:Saponins; D012710:Serum Albumin, Bovine; D009828:Oleanolic Acid; C025759:saikosaponin D