[Potential of the image analyzer "videotest" for performing microphotometric study in cytology].
Precision features of image analyser "Videotest" and possibilities of its applications for cytophotometric investigations have been analysed. It has been shown that a photometric feature is linear at least to optical density 1.1. Cytophotometry of the Feulgen stained rat hepatocyte nuclei has been performed. Coefficients of variation of DNA for 2c, 4c and 8c nuclei are 7.72, 7.65 and 6.50%, respectively, and ratios between average values of these nuclei are 1.00:2.06:4.12. Possible causes of errors of digital television equipment for cytophotometry are discussed.
Stein
G I
GI
Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Panteleev
V G
VG
Povarkova
A V
AV
Kudriavtsev
B N
BN
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D003584:Cytological Techniques; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D016014:Linear Models; D008099:Liver / Q000648:ultrastructure; D015591:Microspectrophotometry; D051381:Rats; D014741:Video Recording
Vozmozhnosti analizatora izobrazheniĭ "videotest" dlia provedeniia mikrofotometricheskikh issledovaniĭ v tsitologii.