title: Hyperthermophilic redox chemistry: a re-evaluation.
abstract: The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution.
authors.affiliation: Wageningen University and Research Centre, Department of Biomolecular Sciences, The Netherlands. peter.leonhagedoorn@epr.bc.wau.nl
authors.forename: P L
authors.initials: PL
authors.lastname: Hagedoorn
authors.forename: M C
authors.initials: MC
authors.lastname: Driessen
authors.forename: M
authors.initials: M
authors.lastname: van den Bosch
authors.forename: I
authors.initials: I
authors.lastname: Landa
authors.forename: W R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000378:metabolism; D012416:Rubredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D017186:Titrimetry
chemical_list: D005288:Ferredoxins; D012416:Rubredoxins