title: Heterogeneously vancomycin-resistant Staphylococcus epidermidis strain causing recurrent peritonitis in a dialysis patient during vancomycin therapy.
abstract: Methicillin-resistant Staphylococcus epidermidis (MRSE) was recovered over a 2-month period from the dialysis fluid of a peritoneal dialysis (PD) patient who experienced recurrent episodes of peritonitis during therapeutic and prophylactic use of vancomycin. Characterization of five consecutive MRSE isolates by molecular and microbiological methods showed that they were representatives of a single strain, had reduced susceptibility to vancomycin, did not react with DNA probes specific for the enterococcal vanA or vanB gene, and showed characteristics reminiscent of the properties of a recently described vancomycin-resistant laboratory mutant of Staphylococcus aureus. Cultures of these MRSE isolates were heterogeneous: they contained-with a frequency of 10(-4) to 10(-5)-bacteria for which vancomycin MICs were high (25 to 50 microg/ml) which could easily be selected to "take over" the cultures by using vancomycin selection in the laboratory. In contrast, the five consecutive MRSE isolates recovered from the PD patient during virtually continuous vancomycin therapy showed no indication for a similar enrichment of more resistant subpopulations, suggesting the existence of an "occult" infection site in the patient (presumably at the catheter exit site) which was not accessible to the antibiotic.
authors.affiliation: Laboratory of Microbiology, The Rockefeller University, New York, New York 10021, USA.
authors.forename: K
authors.initials: K
authors.lastname: Sieradzki
authors.forename: R B
authors.initials: RB
authors.lastname: Roberts
authors.forename: D
authors.initials: D
authors.lastname: Serur
authors.forename: J
authors.initials: J
authors.lastname: Hargrave
authors.forename: A
authors.initials: A
authors.lastname: Tomasz
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology / Q000627:therapeutic use*; D019072:Antibiotic Prophylaxis; D005260:Female; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D010530:Peritoneal Dialysis; D010538:Peritonitis / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control; D012008:Recurrence; D013203:Staphylococcal Infections / Q000188:drug therapy / Q000382:microbiology* / Q000517:prevention & control; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000302:isolation & purification / Q000472:pathogenicity; D014640:Vancomycin / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D000900:Anti-Bacterial Agents; D014640:Vancomycin