Translocation of acylated pardaxin into cells.
Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated 125I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated 125I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules.
Paul
Y
Y
Institute of Toxicology, Medical School of Hannover, Germany.
Weiss
A
A
Adermann
K
K
Erdmann
G
G
Kassebaum
C
C
Lazarovici
P
P
Hochman
J
J
Wellhöner
H
H
D000215:Acylation; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001692:Biological Transport; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002466:Cell Nucleolus / Q000378:metabolism*; D019439:Chromaffin Cells / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005397:Fish Venoms / Q000378:metabolism* / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D018613:Microscopy, Confocal; D013696:Temperature; D013997:Time Factors
D005397:Fish Venoms; D005452:Fluoresceins; C017603:pardaxin; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein