Effect of in vitro incubation on spontaneous acrosome reaction in fresh and cryopreserved human spermatozoa.
OBJECTIVE
Capacitation and acrosome reaction are prerequisites for fertilization. However, in vitro capacitation is not necessary for an agonist-induced acrosome reaction. We studied whether in vitro capacitation is important in spontaneous acrosome reaction and analyzed how capacitation before cryopreservation influences the acrosomal status of thawed spermatozoa.


METHODS
Semen specimens from normal donors (n = 15) were processed by the swim-up technique and divided into two aliquots. One aliquot was capacitated (capacitation induced) for three hours by incubation in a modified-BWW medium with 3% HSA at 37 degrees C under 5% carbon dioxide. The other aliquot did not receive any treatment. Both aliquots were analyzed by CASA to assess the capacitation status of the spermatozoa and then cryopreserved. Spontaneous acrosome reaction was assessed by FITC-PNA lectin before and after cryopreservation. Sperm viability was measured using Hoechst-33258 stain.


RESULTS
Before freezing, the frequency of spontaneous acrosome reaction was higher in the capacitation-induced sperm preparation (median, 20.5% [interquartile range, 17.2-37.8]) than in swim-up-induced specimens (median, 10.6% [range, 4.8-23.2]; P <.001). The percentage of viable cells showing acrosome reaction increased after cryopreservation in both swim-up-induced specimens (median, 241.4% [interquartile range, 37.1-678.6]; P <.001) and capacitation-induced specimens (median, 48.2% [range, 6.1-63.3]; P = 0.002). Although this increase was higher in the swim-up-induced specimens (P = 0.002), frequency of postthaw spontaneous acrosome reaction was similar in both groups (P = 0.18).


CONCLUSIONS
We conclude that sperm capacitation significantly optimizes the acrosome reaction. However, a small proportion of normal spermatozoa do not require capacitation to undergo spontaneous acrosome reaction in vitro. After cryopreservation, the percentage of spermatozoa that had intact acrosomes was similar in both groups, despite the fact that one aliquot underwent prefreeze capacitation. These findings suggest that the acrosome reaction may involve complex mechanism(s) rather than a physiological change induced by capacitation.
Esteves
S C
SC
Department of Urology, The Cleveland Clinic Foundation, Ohio 44195, USA.
Sharma
R K
RK
Thomas
A J
AJ
Agarwal
A
A
D020101:Acrosome Reaction; D015925:Cryopreservation; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D015588:Observer Variation; D012016:Reference Values; D015203:Reproducibility of Results; D012662:Semen Preservation; D013075:Sperm Capacitation; D013094:Spermatozoa / Q000502:physiology*