The effect of ovarian steroids on epithelial ciliary beat frequency in the human Fallopian tube.
Using a method that detects variations in light intensity we have studied the effect of ovarian steroids on human Fallopian tube epithelial ciliary beat frequency in vitro. We have found that baseline ciliary beat frequency averages between 5-6 Hz. Cilia from ampullary segments of the Fallopian tube beat significantly faster (5.4 Hz+/-0.2) than those from fimbrial segments (4.8 Hz+/-0.2). There was no significant difference in baseline ciliary beat frequency at any other anatomical site in the Fallopian tube. Incubation with progesterone (10 micromol/l) suppresses human Fallopian tube epithelial ciliary beat frequency by 40-50%. This inhibition was observed at similar magnitudes in all Fallopian tubes studied irrespective of anatomical site. Progesterone-induced reductions in ciliary beat frequency were concentration dependent and prevented by the progesterone receptor antagonist mifepristone (RU486). Oestradiol alone (10 micromol/l) had no effect on ciliary beat frequency at any anatomical site in the Fallopian tube but did prevent the reduction in ciliary beat frequency seen with progesterone when tissues were incubated with these two steroids together.
Mahmood
T
T
Academic Department of Obstetrics, Gynaecology and Reproductive Physiology, St Bartholomew's and the Royal London School of Medicine, The Royal London Hospital, Whitechapel, UK.
Saridogan
E
E
Smutna
S
S
Habib
A M
AM
Djahanbakhch
O
O
D002923:Cilia / Q000187:drug effects* / Q000502:physiology*; D004848:Epithelium / Q000648:ultrastructure; D004958:Estradiol / Q000494:pharmacology*; D005187:Fallopian Tubes / Q000648:ultrastructure*; D005260:Female; D006727:Hormone Antagonists / Q000494:pharmacology; D006801:Humans; D015735:Mifepristone / Q000494:pharmacology; D011374:Progesterone / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D006727:Hormone Antagonists; D015735:Mifepristone; D011374:Progesterone; D004958:Estradiol