title: Combined inhibitory effect of bovine immune whey and peroxidase-generated hypothiocyanite against glucose uptake by Streptococcus mutans.
abstract: Immune whey product was obtained from Streptococcus mutans- and Streptococcus sobrinus-immunized cows. Hypothiocyanite (HOSCN/OSCN-) was generated in VMG-buffer, pH 5.5 or 6.5, by bovine milk lactoperoxidase, KSCN and hydrogen peroxide. The glucose incorporation by late-log cells of S. mutans 10449, serotype c, was followed by measuring the uptake of 14C-glucose at 37 degrees C. At pH 5.5 and 6.5 both immune whey product and HOSCN/OSCN- dose-dependently inhibited glucose uptake. The inhibition by their combination was additive if bacterial cells were treated with HOSCN/OSCN- before exposed to immune whey product. In contrast to immune whey product, the control product from sham-immunized cows increased the glucose uptake even when added simultaneously with HOSCN/OSCN-. However, when bacterial cells were pretreated with HOSCN/OSCN- an enhanced inhibitory effect was observed also with control product. The results indicate that colostral proteins from S. mutants- or S. sobrinus-immunized cows inhibit glucose uptake and that the effect is enhanced by pretreatment with lactoperoxidase-generated HOSCN/OSCN-.
authors.affiliation: Institute of Dentistry, University of Turku, Finland.
authors.forename: V
authors.initials: V
authors.lastname: Loimaranta
authors.forename: J
authors.initials: J
authors.lastname: Tenovuo
authors.forename: H
authors.initials: H
authors.lastname: Korhonen
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology / Q000494:pharmacology; D002417:Cattle; D003731:Dental Caries / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology / Q000494:pharmacology; D007784:Lactoperoxidase / Q000378:metabolism; D008894:Milk Proteins / Q000276:immunology* / Q000494:pharmacology; D013295:Streptococcus mutans / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D013861:Thiocyanates / Q000494:pharmacology*; D000067816:Whey Proteins
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D008894:Milk Proteins; D013861:Thiocyanates; D000067816:Whey Proteins; C014607:hypothiocyanite ion; D007784:Lactoperoxidase; D005947:Glucose