title: Bmp-2 downstream targets in mesenchymal development identified by subtractive cloning from recombinant mesenchymal progenitors (C3H10T1/2).
abstract: ABmp-dependent in vitro model was used to identify cDNAs during the manifestation of mesenchymal lineages. This model involves the recombinant expression of Bmps (Bmp-2, Bmp-4-7) in murine mesenchymal C3H10T1/2 progenitors, which leads to the differentiation into three lineages: the osteogenic, the chondrogenic and the adipogenic lineage, albeit in varying efficiencies. By subtractive cloning, 21 Bmp-2-regulated cDNAs from C3H10T1/2 mesenchymal progenitors were identified; 20 were related to known sequences and 1 was not. During mouse embryonic development, many of these cDNAs are expressed in chondrogenic, osteogenic, and in adipogenic tissues. Novel findings include a G0/G1 switch gene (G0S2), which was demonstrated to be predominantly expressed in adipose tissue during late murine embryonic development. Furthermore, the membrane-standing glycoprotein autotaxin (ATX) is expressed, at precartilage condensations, joint regions, and during tooth development. An as yet undescribed cDNA, 29A, which encodes a putative secreted factor, is expressed in developing osteo-/chondrogenic tissues of vertebrae, ribs, tooth, and the limb bud. C3H10T1/2-progenitors, therefore, may serve as a legitimate model for the investigation of the Bmp-mediated events during mesenchymal differentiation.
authors.affiliation: Gesellschaft für Biotechnologische Forschung, Growth Factors and Receptors, Braunschweig, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Bächner
authors.forename: M
authors.initials: M
authors.lastname: Ahrens
authors.forename: D
authors.initials: D
authors.lastname: Schröder
authors.forename: A
authors.initials: A
authors.lastname: Hoffmann
authors.forename: J
authors.initials: J
authors.lastname: Lauber
authors.forename: N
authors.initials: N
authors.lastname: Betat
authors.forename: P
authors.initials: P
authors.lastname: Steinert
authors.forename: L
authors.initials: L
authors.lastname: Flohé
authors.forename: G
authors.initials: G
authors.lastname: Gross
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism*; D022762:CCAAT-Enhancer-Binding Proteins; D019070:Cell Lineage; D019902:Chondrocytes; D018076:DNA, Complementary; D004268:DNA-Binding Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D008648:Mesoderm; D051379:Mice; D008809:Mice, Inbred C3H; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D010006:Osteoblasts; D013234:Stem Cells; D016212:Transforming Growth Factor beta; D015854:Up-Regulation
chemical_list: C525719:Bmp2 protein, mouse; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D022762:CCAAT-Enhancer-Binding Proteins; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016212:Transforming Growth Factor beta