title: Persistence of translocations after accidental exposure to ionizing radiation.
abstract: OBJECTIVE
  To assess the validity of translocations for retrospective biodosimetry using FISH chromosome painting of peripheral lymphocytes in repeated samples of people accidentally exposed to radiation.
  
  
  METHODS
  Blood samples from five people exposed to mainly whole-body irradiation of gamma-rays from a radiation accident in Estonia in 1994 were taken at 2-4 month intervals. A total of eight samplings were carried out, including one directly after the accident. Fluorescence in situ hybridization with probes for chromosomes 1, 2 and 4 was performed on metaphase preparations from 48 h whole blood cultures; 1500 cells were scored from each individual per time point.
  
  
  RESULTS
  Translocations remained relatively stable during 2 years after exposure in all subjects. A noticeable decrease in complete translocation yields and a significant decrease in incomplete translocations were observed in one person. In addition to whole-body exposure, he had also been exposed to partial-body irradiation. Due to the overall persistence of translocations, dose estimates were very similar throughout the 2 year period. A rapid decline in dicentric frequencies was noted during the first year after exposure.
  
  
  CONCLUSIONS
  The results suggest that during 2 years the yield of translocations in peripheral lymphocytes remained at a constant level after whole-body exposure. This finding supports the use of translocations for retrospective dosimetry, at least within this relatively short period of follow-up. In the case of partial-body irradiation, however, the elimination of co-existing unstable aberrations reduced the translocation yield over time. Follow-up will be continued in order to determine the stability of translocations over longer times.
authors.affiliation: Radiation and Nuclear Safety Authority, Helsinki, Finland.
authors.forename: C
authors.initials: C
authors.lastname: Lindholm
authors.forename: M
authors.initials: M
authors.lastname: Tekkel
authors.forename: T
authors.initials: T
authors.lastname: Veidebaum
authors.forename: T
authors.initials: T
authors.lastname: Ilus
authors.forename: S
authors.initials: S
authors.lastname: Salomaa
mesh_terms: D002588:Cesium Radioisotopes; D020223:Chromosome Painting; D004957:Estonia; D005500:Follow-Up Studies; D006801:Humans; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D011829:Radiation Dosage; D011839:Radiation, Ionizing; D018788:Radioactive Hazard Release; D013997:Time Factors; D014178:Translocation, Genetic
chemical_list: D002588:Cesium Radioisotopes