Colloidal silica-coated tissue culture dishes for primary cell cultures: growth of rabbit renal proximal tubule cells.
The use of colloidal silica as a substratum for primary cultures of differentiated cells has significant advantages over classic tissue culture polystyrene. In this report, the growth and the level of expression of differentiated function of primary rabbit renal proximal tubule (RPT) cell cultures on colloidal silica is examined, using hormonally defined serum-free medium. Primary RPT cells grew to confluence more rapidly on colloidal silica than on tissue culture polystyrene (TC+). Moreover, following three passages, the RPT cells increased in number threefold more than parallel cultures on TC+. The morphology of primary RPT cells on colloidal silica were found by means of transmission electron microscopy to possess a polarized morphology with a brush border, and differentiated markers were retained even after passaging, including the Na+/glucose cotransport system and Glut 7.
Taub
M
M
Biochemistry Department, State University of New York at Buffalo 14214, USA.
Axelson
E
E
Park
J H
JH
D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation; D002455:Cell Division; D048429:Cell Size; D003102:Colloids; D046508:Culture Techniques / Q000379:methods*; D007687:Kidney Tubules, Proximal / Q000737:chemistry / Q000166:cytology* / Q000648:ultrastructure; D008562:Membrane Glycoproteins / Q000032:analysis; D008854:Microscopy, Electron; D009004:Monosaccharide Transport Proteins / Q000032:analysis; D011817:Rabbits; D012822:Silicon Dioxide; D051269:Sodium-Glucose Transporter 1
D003102:Colloids; D008562:Membrane Glycoproteins; D009004:Monosaccharide Transport Proteins; D051269:Sodium-Glucose Transporter 1; D012822:Silicon Dioxide