Effect of hibernation on responses of hamster vas deferens to sympathetic nerve stimulation and exogenous neurotransmitters.
The present study investigated the responses of the vas deferens to sympathetic nerve stimulation and exogenous neurotransmitters taken from golden hamsters which had undergone 8 weeks of hibernation, 2 h of arousal from hibernation, those exposed to the cold but which failed to hibernate and age-matched control animals. Electrical field stimulation (EFS) of the vas deferens from each group produced frequency-dependent, tetrodotoxin-sensitive contractions. Contractions elicited by low frequencies of EFS in the hibernating group were significantly greater than in the other groups in the absence of any blocking agents. In the presence of the alpha1-adrenoceptor antagonist prazosin (3 microM) responses from all groups were reduced by approximately 40%, with the residual responses from the hibernating group being somewhat increased compared to the other groups. In the presence of the P2X receptor antagonist pyridoxalphosphate-6-azophenyl-2'4'-disulphonic acid (30 microM), there was no significant difference in responses from all 4 groups. Exogenously applied beta, gamma-methylene ATP (beta,gamma-meATP; 0.1-300 microM), a P2X receptor agonist, and noradrenaline (NA; 30 nM(-1) mM) both caused transient concentration-dependent contractions in all groups of animals. Contractions to beta,gamma-meATP at concentrations above 0.3 microM, and NA above 0.3 microM in the hibernating animals were statistically significantly greater than the cold- and age-matched control groups, although not significantly different from the aroused group. This study has shown postjunctional increases in responses to beta,gamma-meATP and NA as a result of hibernation, possible explanations for these increases are discussed.
Knight
G E
GE
Autonomic Neuroscience Institute, Royal Free Hospital School of Medicine, London, UK.
Burnstock
G
G
D000255:Adenosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D001831:Body Temperature / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D006224:Cricetinae; D004558:Electric Stimulation; D006605:Hibernation / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D008647:Mesocricetus; D009119:Muscle Contraction / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000502:physiology*; D014649:Vas Deferens / Q000187:drug effects / Q000294:innervation / Q000502:physiology*
D018377:Neurotransmitter Agents; D000255:Adenosine Triphosphate; C002630:alpha,beta-methyleneadenosine 5'-triphosphate; D009638:Norepinephrine