title: The cell adhesion molecule E-cadherin is widely expressed in human atherosclerotic lesions.
abstract: OBJECTIVE
  Various cell adhesion molecules are expressed in atherogenesis and the significance of their involvement in atherosclerotic lesion formation is well appreciated. In the present work, we examined whether the Ca(2+)-dependent cell adhesion molecule E-cadherin is also involved in atherogenesis.
  
  
  METHODS
  Specimens of carotid artery and aorta were obtained at operation. Expression of E-cadherin was studied by an immunohistochemical method. The nature of E-cadherin-expressing cells was examined by comparative analysis of consecutive sections and by a double immunostaining procedure. An immunohistochemical approach was also applied to examine how the accumulation of oxidised low density lipoproteins (LDL) by intimal cells is associated with E-cadherin expression.
  
  
  RESULTS
  No E-cadherin+ cells were found in normal non-atherosclerotic intima but E-cadherin+ cells were present in 96% of the atherosclerotic lesions. In atherosclerotic intima, E-cadherin was expressed by intimal cells showing varying degrees of transformation into foam cells. These E-cadherin+ cells also contained oxidised LDL in their cytoplasm. Differing numbers of CD68+ foam cells (15% to 60%) expressed E-cadherin but all the CD68+ macrophages without signs of transformation into foam cells were negative for E-cadherin. Neither smooth muscle cells nor foam cells of smooth muscle cell origin (smooth muscle alpha-actin+) were found to be positive for E-cadherin. T-cells (CD3+) and endothelial cells (von Willebrand factor+) were also negative for E-cadherin. Only a few vascular dendritic cells (S-100+) expressed E-cadherin and their expression was weak. We also found that a large proportion (40% to 85%) of E-cadherin+ cells did not stain with any cell-type specific markers.
  
  
  CONCLUSIONS
  The finding that E-cadherin is expressed in atherosclerotic lesions expands our knowledge of cell adhesion molecules involved in atherogenesis. That E-cadherin is expressed in intimal cells transforming into foam cells suggests that lipid accumulation might be associated with the alteration and reorganisation of cell-to-cell interactions in atherogenesis. The present observations might assist in understanding the mechanisms associated with intracellular lipid accumulation.
authors.affiliation: Surgical Professorial Unit, St. Vincent's Hospital, Darlinghurst NSW, Australia.
authors.forename: Y V
authors.initials: YV
authors.lastname: Bobryshev
authors.forename: R S
authors.initials: RS
authors.lastname: Lord
authors.forename: T
authors.initials: T
authors.lastname: Watanabe
authors.forename: T
authors.initials: T
authors.lastname: Ikezawa
mesh_terms: D000328:Adult; D000368:Aged; D001011:Aorta / Q000737:chemistry*; D001161:Arteriosclerosis / Q000378:metabolism*; D015820:Cadherins / Q000032:analysis*; D002339:Carotid Arteries / Q000737:chemistry*; D005487:Foam Cells / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D008077:Lipoproteins, LDL / Q000032:analysis; D008875:Middle Aged; D017539:Tunica Intima / Q000737:chemistry
chemical_list: D015820:Cadherins; D008077:Lipoproteins, LDL