[Relation of Lac promotor and the expression of cholera toxin subunit B gene in recombinant Escherichia coli MM2].
Effects of different carbon sources including glucose, lactate and acetate and IPTG induction on the expression of ctb gene, which is on the downstream of lac promotor, in recombinant Escherichia coli MM2 were studied. In medium YC were added 0.048mol/L glucose, 0.102mol/L lactate or 0.167mol/L acetate which separately produce the same energy in the condition of complete oxidization. Addition of glucose largely decreased the expression level of ctb gene because of decrease of pH during culture process. Addition of lactate increased the expression level of ctb gene by 1.15 fold and did not inhibit the growth of MM2 strain. Addition of acetate increasd the expression level of ctb gene by 0.97 fold and inhibited the growth of MM2 strain. Induction by IPTG at different time and different concentration did not increase the expression level of ctb gene, so the lac promotor had no or a little influence upon the expression of ctb gene in recombinant MM2 strain.
Fang
H
H
Institute of Biotechnology, Academy of Military Medical Sciences, Beijing.
Zhao
S
S
Yu
G
G
Ma
Q
Q
D000085:Acetates / Q000494:pharmacology; D002772:Cholera Toxin / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007763:Lac Operon; D019344:Lactic Acid / Q000494:pharmacology; D010446:Peptide Fragments / Q000096:biosynthesis* / Q000235:genetics
D000085:Acetates; D010446:Peptide Fragments; D019344:Lactic Acid; C050310:cholera toxin, B subunit (50-64); D002772:Cholera Toxin; D005947:Glucose