title: Secondary structure and molecular analysis of interstrain variability in the P5 outer-membrane protein of non-typable Haemophilus influenzae isolated from diverse anatomical sites.
abstract: The sequence of the non-typable Haemophilus influenzae (NTHi) P5 outer-membrane protein from a range of clinical isolates is presented and represents the first analysis of the heterogeneity in P5 from NTHi isolates from diverse anatomical sites. The basis of the previously observed inter-strain variation in the electrophoretic mobility is attributed to heterogeneity in three hypervariable regions. Alignment of the P5 sequences identified regions which are highly conserved and align with the transmembrane region predicted for the homologous Escherichia coli protein, OmpA. Variable regions correspond to surface-exposed loops, of which the first loop falls into subclasses. However, these subclasses fail to correlate with anatomical predisposition. Although P5 has been proposed as a fimbrial protein composed of coiled coils, both structural analysis by circular dichroism of purified P5 and computer analysis of the multiply aligned sequences predict a high proportion of beta strand with no evidence of coiled coil structure. A detailed model of P5 is presented.
authors.affiliation: Research Centre for Human and Biomedical Science, Faculty of Applied Science, University of Canberra, ACT, Australia.
authors.forename: D C
authors.initials: DC
authors.lastname: Webb
authors.forename: A W
authors.initials: AW
authors.lastname: Cripps
mesh_terms: D000595:Amino Acid Sequence; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D002942:Circular Dichroism; D003228:Conjunctiva / Q000382:microbiology; D004269:DNA, Bacterial / Q000737:chemistry; D004423:Ear / Q000382:microbiology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D014644:Genetic Variation; D006193:Haemophilus influenzae / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008969:Molecular Sequence Data; D010256:Paranasal Sinuses / Q000382:microbiology; D010614:Pharynx / Q000382:microbiology; D017433:Protein Structure, Secondary; D016415:Sequence Alignment; D013183:Sputum / Q000382:microbiology; D014621:Vagina / Q000382:microbiology
chemical_list: D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial; C082912:outer membrane protein P5, Haemophilus