title: Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium.
abstract: A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869-3877, 1998).
authors.affiliation: Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, 10117 Berlin, Germany.
authors.forename: F
authors.initials: F
authors.lastname: von Wintzingerode
authors.forename: B
authors.initials: B
authors.lastname: Selent
authors.forename: W
authors.initials: W
authors.lastname: Hegemann
authors.forename: U B
authors.initials: UB
authors.lastname: Göbel
mesh_terms: D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002722:Chlorobenzenes / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D002722:Chlorobenzenes; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C036720:trichlorobenzene