Restoration of the p16 gene is related to increased radiosensitivity of p16-deficient lung adenocarcinoma cell lines.
To assess the role played by p16 gene expression in the radiosensitivity of human lung cancers, we transferred exogenous p16 genes into p16-deficient H460 and A549 lung adenocarcinoma cell lines and compared the cell survival curve in vitro after irradiation. The surviving fraction of the p16-transfected A549p16 and H460p16 cells that expressed exogenous p16 mRNA or protein was lower than those of the parental and negative control cells. The rapid exit of the p16-transfected cells from the G2/M phase in the cell cycle, both before and after irradiation, possibly contributes to the increased radiosensitivity of our experimental p16-transfected lung adenocarcinoma cell lines. We conclude that exogenous p16 gene may be another important factor controlling the intrinsic cellular radiosensitivity of cancer.
Fu
X Y
XY
Department of Radiotherapy, Cancer Hospital, Shanghai Medical University, PR China.
Zhang
S W
SW
Ran
R Q
RQ
Shen
Z H
ZH
Gu
J X
JX
Cao
S L
SL
D000230:Adenocarcinoma / Q000235:genetics* / Q000532:radiotherapy; D002453:Cell Cycle; D002470:Cell Survival / Q000528:radiation effects; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D018014:Gene Transfer Techniques; D019942:Genes, p16; D008175:Lung Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D011836:Radiation Tolerance; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
D019941:Cyclin-Dependent Kinase Inhibitor p16; D011994:Recombinant Proteins