title: Pregnancy following intracytoplasmic sperm injection from an HIV-1-seropositive man.
abstract: The first pregnancy achieved in a seronegative woman following in-vitro fecundation through intracytoplasmic sperm (ICSI) injection from a man with autoimmune deficiency syndrome (AIDS; HIV-1 carrier) is reported. The semen was prepared by PureSperm and swim-up techniques. Some of the motile spermatozoa obtained were used to detect the presence of HIV-1 using the polymerase chain reaction technique. HIV-1 in DNA or RNA form was not detected using this technique. The remaining spermatozoa were frozen. Ovarian stimulation in the woman was performed with long-protocol analogues and gonadotrophins. Thirteen mature oocytes were recovered, into which the thawed spermatozoa were microinjected. Nine embryos were obtained. Four were frozen, four transferred and one discarded. The woman became pregnant. Analyses for HIV-1 in the woman, performed in the first and third months of pregnancy, gave negative results. This case provides further experience with washed semen of sufficient quality for performing artificial insemination in HIV-1-serodiscordant couples (101 inseminations, 31 pregnancies, 28 deliveries, 37 babies, all healthy). In women with obstructed Fallopian tubes, or when the semen is not of sufficient quality for artificial insemination techniques to be performed, ICSI can be carried out using frozen, HIV-1-free semen.
authors.affiliation: Instituto de Reproducción CEFER (Centro Médico TEKNON), Barcelona, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Marina
authors.forename: F
authors.initials: F
authors.lastname: Marina
authors.forename: R
authors.initials: R
authors.lastname: Alcolea
authors.forename: J
authors.initials: J
authors.lastname: Nadal
authors.forename: R
authors.initials: R
authors.lastname: Expósito
authors.forename: J
authors.initials: J
authors.lastname: Huguet
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000517:prevention & control / Q000635:transmission; D000328:Adult; D002469:Cell Separation; D004279:DNA, Viral / Q000032:analysis; D004624:Embryo Transfer; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D015483:HIV Antibodies / Q000097:blood; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D008297:Male; D008845:Microinjections; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012367:RNA, Viral / Q000032:analysis; D013094:Spermatozoa / Q000821:virology
chemical_list: D004279:DNA, Viral; D015483:HIV Antibodies; D012367:RNA, Viral