Modulation of apoptosis in human lymphocytes by adenosine analogues.
We previously described that 2-chloroadenosine (2CA) and 2-chloro-2'-deoxyadenosine (2CdA) induced apoptosis in human peripheral blood mononuclear cells (PBMC). In this study we tested different adenosine analogues on PBMC and we found that the modifications introduced in the 2CA structure prevented the molecule from exerting its apoptotic effect. On the other hand, substitutions on 2CdA are tolerated, although with a significant decrease in activity.
Barbieri
D
D
Department of Biomedical Sciences, University of Modena, Italy.
Franceschi
C
C
Camaioni
E
E
Costanzi
S
S
Vittori
S
S
Volpini
R
R
Cristalli
G
G
D015762:2-Chloroadenosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D003839:Deoxyadenosines / Q000737:chemistry / Q000494:pharmacology; D005434:Flow Cytometry; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000502:physiology; D008956:Models, Chemical; D011419:Propidium / Q000494:pharmacology; D013329:Structure-Activity Relationship
D003839:Deoxyadenosines; D015762:2-Chloroadenosine; C036854:2'-chloro-2'-deoxyadenosine; D011419:Propidium