CD40 regulation of death domains containing receptors and their ligands on lymphoma B cells.
Within the tumour necrosis factor (TNF) family the induction of apoptosis is restricted to some ligand-receptors pairs, including TNF-TNF receptor type I (TNFRI/p55), FasL-Fas, TNF-related apoptosis-inducing ligand (TRAIL) and its death-receptors (DR)-4 and -5. The pair CD40L-CD40 belongs to the same family but rescues B cells from apoptosis. To investigate how these opposing actions are cross-linked, purified follicular lymphoma (FL) cells were activated upon a human CD40L-transfected murine fibroblastic layer, then RNA messengers for the above molecules were analysed using RT-PCR. The observed down-modulation of TRAIL and up-regulation of TNF and Fas transcripts might account for CD40-CD40L-mediated FL cell survival.
Ribeiro
P
P
Service d'Hématologie, Centre Hospitalier Lyon-Sud, Hospices Civils de Lyon et UPRES-JE 1879 Hémopathies Lymphoïdes Malignes, France.
Renard
N
N
Warzocha
K
K
Charlot
C
C
Jeandenant
L
L
Callet-Bauchu
E
E
Coiffier
B
B
Salles
G
G
D015703:Antigens, CD / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D001402:B-Lymphocytes / Q000473:pathology*; D019013:CD40 Antigens / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology; D015536:Down-Regulation; D053222:Fas Ligand Protein; D006801:Humans; D008224:Lymphoma, Follicular / Q000473:pathology*; D008562:Membrane Glycoproteins / Q000378:metabolism; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor / Q000378:metabolism; D047888:Receptors, Tumor Necrosis Factor, Type I; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism
D015703:Antigens, CD; D019013:CD40 Antigens; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D053220:Receptors, TNF-Related Apoptosis-Inducing Ligand; D018124:Receptors, Tumor Necrosis Factor; D047888:Receptors, Tumor Necrosis Factor, Type I; C105190:TNFRSF10A protein, human; C505639:TNFRSF10B protein, human; D019014:fas Receptor