title: Three patients with autoimmune cholangiopathy treated with prednisolone.
abstract: We describe three patients with autoimmune cholangiopathy, i.e., anti-mitochondrial antibody-negative and anti-nuclear antibody-positive primary biliary cirrhosis, who were treated with prednisolone. Serum anti-mitochondrial antibody and anti-pyruvate dehydrogenase-E2 component antibody were determined by immunofluorescence of frozen sections and enzyme-linked immunosorbent assay, respectively. Immunoblotting using mitochondria prepared from rat liver was performed to analyze anti-mitochondrial antibody in detail. Serum from one patient reacted with a 48-kilodalton protein, but sera from the other two patients failed to react with the mitochondrial proteins. There was a marked improvement in liver function test results after prednisolone treatment. Before treatment, liver biopsy in all three patients showed histological features of primary biliary cirrhosis with hepatocellular necrosis. Repeat biopsy during treatment showed marked amelioration of hepatocellular damage in all three patients, although bile duct involvement persisted in two patients. These findings suggest that prednisolone is an effective treatment for hepatocellular damage in patients with autoimmune cholangiopathy, but has little impact on the bile duct involvement.
authors.affiliation: Third Department of Internal Medicine, Ehime University School of Medicine, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Masumoto
authors.forename: T
authors.initials: T
authors.lastname: Ninomiya
authors.forename: K
authors.initials: K
authors.lastname: Michitaka
authors.forename: N
authors.initials: N
authors.lastname: Horiike
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: S M
authors.initials: SM
authors.lastname: Akbar
authors.forename: M
authors.initials: M
authors.lastname: Abe
authors.forename: M
authors.initials: M
authors.lastname: Onji
mesh_terms: D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D001327:Autoimmune Diseases / Q000188:drug therapy* / Q000473:pathology; D001707:Biopsy, Needle; D015209:Cholangitis, Sclerosing / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006529:Hepatomegaly / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D006801:Humans; D008111:Liver Function Tests; D008875:Middle Aged; D011239:Prednisolone / Q000627:therapeutic use*; D016896:Treatment Outcome
chemical_list: D000893:Anti-Inflammatory Agents; D011239:Prednisolone