Importance of pre-enrichment media for isolation of Salmonella spp. from swine and poultry.
The performance of two new (1-day) culture methods, Salmonella Enrichment Broth (SEB) and Revive, and an alternative pre-enrichment broth, designated Universal pre-enrichment broth (UB), was compared to the internationally accepted buffered peptone water (BPW). The study was directed towards detection of Salmonella in 100 faecal samples from porcine and 100 neck-skin samples from poultry. The sensitivity (number of positive cases per method among all the positive cases) of the conventional pre-enrichment in BPW was found to be 0.77 for swine and 0.66 for poultry samples, while a combination of the BPW method with parallel pre-enrichment of the same sample in UB resulted in high sensitivity for swine (0.92) and poultry (0.95) samples. A 2-h pre-enrichment in the non-selective Revive, followed by overnight enrichment in selective broth, resulted in a low sensitivity, particularly for the neck-skin samples (0.16, P = 0.001). The SEB method in the porcine samples resulted in a sensitivity (0.71) comparable to the standard method (P = 0.31). In conclusion, additional pre-enrichment of samples in UB may substantially increase the culture sensitivity. During routine screening of large numbers of samples, it may be advantageous to use SEB rather than standard culturing.
Hoorfar
J
J
Department of Microbiology, Danish Veterinary Laboratory, Copenhagen, Denmark. jho@svs.dk
Baggesen
D L
DL
D000003:Abattoirs; D000818:Animals; D001431:Bacteriological Techniques / Q000592:standards; D002645:Chickens; D003470:Culture Media; D004372:Ducks; D005243:Feces / Q000382:microbiology; D009333:Neck / Q000382:microbiology; D011200:Poultry / Q000382:microbiology*; D012475:Salmonella / Q000302:isolation & purification*; D012481:Salmonella Infections, Animal / Q000175:diagnosis; D012680:Sensitivity and Specificity; D013552:Swine / Q000382:microbiology*
D003470:Culture Media