Enhanced murine CD4+ T cell responses induced by the CD2 ligand CD48.
The physiological functions of murine CD2 and its ligand CD48 are uncertain. We have examined the role of the CD2-CD48 interaction in murine T cell activation using a series of Chinese hamster ovary (CHO) cell transfectants. CHO cells expressing I-Ad together with CD48 induced more potent activation of OVA-specific, I-Ad-restricted DO11.10-transgenic T cells than CHO cells expressing I-Ad alone. CD48 augmented proliferation and IL-2 production in response to antigen. The enhancing effect of CD48 was of the same magnitude as that seen for CD80 (B7-1). Conjugate assays revealed the ability of CD48 to increase adhesion between T cells and CHO transfectants. The enhancing effects of CD48 on T cell-antigen-presenting cell adhesion and T cell activation were inhibited by anti-CD2 monoclonal antibody. This report provides the first evidence that the CD2 ligand CD48 contributes to the interactions of murine CD4+ T cells with antigen-presenting cells.
Latchman
Y
Y
Department of Immunology, Imperial College School of Medicine, Hammersmith Hospital, London, GB.
Reiser
H
H
D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology*; D018801:CD2 Antigens / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D000071178:CD48 Antigen; D016466:CHO Cells; D006224:Cricetinae; D003602:Cytotoxicity, Immunologic; D008024:Ligands; D008213:Lymphocyte Activation; D051379:Mice; D015398:Signal Transduction / Q000276:immunology*; D014162:Transfection
D015703:Antigens, CD; D018801:CD2 Antigens; D000071178:CD48 Antigen; C000604066:Cd48 protein, mouse; D008024:Ligands