title: Isolation and identification of chondroitin sulfates from the mud snail.
abstract: Chondroitin sulfates were isolated from the mud snail. For the quantitative analysis of enzymatic digestion products of isolated chondroitin sulfates, strong anion exchange-high performance liquid chromatography (SAX-HPLC) was performed. By the action of chondroitinase ABC, three unsaturated disaccharides 2-acetamide-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-D-galactose (delta Di-OS), 2-acetamide-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose (delta Di-6S) and 2-acetamide-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-4-O-sulfo-D-galactose (delta Di-4S) were produced from the mud snail chondroitin sulfates. The analysis showed that relative proportion of delta Di-OS/delta Di-6S/delta Di-4S was 58.7/3.1/38.2. The immunomodulating activity of chondroitin sulfate was examined by cell proliferation assay and these results suggest that it might be a immunosuppressant.
authors.affiliation: Department of Chemistry, Konyang University, Nonsan, Chungnam, Korea.
authors.forename: K B
authors.initials: KB
authors.lastname: Lee
authors.forename: J S
authors.initials: JS
authors.lastname: Kim
authors.forename: S T
authors.initials: ST
authors.lastname: Kwak
authors.forename: W
authors.initials: W
authors.lastname: Sim
authors.forename: J H
authors.initials: JH
authors.lastname: Kwak
authors.forename: Y S
authors.initials: YS
authors.lastname: Kim
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002240:Carbohydrate Sequence; D002455:Cell Division / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006868:Hydrolysis; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis; D012908:Snails / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000276:Adjuvants, Immunologic; D009844:Oligosaccharides; D002809:Chondroitin Sulfates