title: [Establishment of mutant strain from human lung cancer cell (A549) with characteristics of resistance to 8-AG and HGPRT deficiency].
abstract: A human lung cancer cell line (A549) mutant with hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency and resistance to 8-AG was established by treatment of the A549 cells with 3 micrograms.ml-1 N-methyl-N'-nitro-nitrosoguanidine (MNNG), and prescreened in 0.25% agarose DMEM semisolid medium containing 20 micrograms.ml-1 8-AG. The mutant cells are resistant to cytotoxic effect of 8-AG and sensitive to hypoxanthine-aminopterin thymidine (HAT) selective medium. The mutant cells can be used as a candidate parental cells to fuse with the somatic cells.
authors.affiliation: Department of Microbiology, Hunan Medical University, Changsha.
authors.forename: Y
authors.initials: Y
authors.lastname: Xiao
authors.forename: C
authors.initials: C
authors.lastname: Hu
authors.forename: K
authors.initials: K
authors.lastname: Shi
authors.forename: M
authors.initials: M
authors.lastname: Luo
mesh_terms: D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001375:Azaguanine / Q000494:pharmacology*; D002459:Cell Fusion; D002461:Cell Line, Transformed; D019008:Drug Resistance, Neoplasm; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency*; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D014407:Tumor Cells, Cultured
chemical_list: D000964:Antimetabolites, Antineoplastic; D008769:Methylnitronitrosoguanidine; D007041:Hypoxanthine Phosphoribosyltransferase; D001375:Azaguanine