Recombinogenic targeting: a new approach to genomic analysis--a review.
Currently, recombinational cloning procedures based upon methods developed for yeast, Saccharomyces cerevisiae, are being exploited for targeted cloning and in-vivo modification of genomic clones. In this review, we will discuss the development of large-insert vectors, homologous recombination-based techniques for cloning and modification, and their application towards functional analysis of genes using transgenic mouse model systems.
Shashikant
C S
CS
Department of Molecular, Cellular and Developmental Biology, Yale University, Kline Biology Tower, PO Box 208103, New Haven, CT 06520, USA.shashi@minerva.cis.yale.edu
Carr
J L
JL
Bhargava
J
J
Bentley
K L
KL
Ruddle
F H
FH
D000818:Animals; D001419:Bacteria / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D005822:Genetic Vectors; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D011995:Recombination, Genetic; D015003:Yeasts / Q000235:genetics*