title: Fluorescent probes for adenosine receptors: synthesis and biology of N6-dansylaminoalkyl-substituted NECA derivatives.
abstract: New fluorescent ligands for adenosine receptors are described; these compounds were obtained by the insertion, in the N6 position of NECA (a potent adenosine agonist), of dansylaminoalkyl moieties with alkyl spacers of increasing carbon chain length (from 3 to 12). Among them, the compound with a C6 alkyl spacer proved to be the most interesting one, showing a marked selectivity for the A1 receptor subtype; furthermore, in fluorescence microscopy assays it proved to be able to visualize and localize this receptor subtype at the level of the molecular layer of the rate cerebellar cortex.
authors.affiliation: GlaxoWellcome Medicines Research Center, Verona, Italy. mmacchia@farm.unipi.it
authors.forename: M
authors.initials: M
authors.lastname: Macchia
authors.forename: F
authors.initials: F
authors.lastname: Salvetti
authors.forename: S
authors.initials: S
authors.lastname: Barontini
authors.forename: F
authors.initials: F
authors.lastname: Calvani
authors.forename: M
authors.initials: M
authors.lastname: Gesi
authors.forename: M
authors.initials: M
authors.lastname: Hamdan
authors.forename: A
authors.initials: A
authors.lastname: Lucacchini
authors.forename: A
authors.initials: A
authors.lastname: Pellegrini
authors.forename: P
authors.initials: P
authors.lastname: Soldani
authors.forename: C
authors.initials: C
authors.lastname: Martini
mesh_terms: D019830:Adenosine-5'-(N-ethylcarboxamide) / Q000031:analogs & derivatives*; D000818:Animals; D003619:Dansyl Compounds / Q000138:chemical synthesis*; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000378:metabolism; D011869:Radioligand Assay; D051381:Rats; D018047:Receptors, Purinergic P1 / Q000032:analysis*; D013329:Structure-Activity Relationship
chemical_list: D003619:Dansyl Compounds; D005456:Fluorescent Dyes; D018047:Receptors, Purinergic P1; D019830:Adenosine-5'-(N-ethylcarboxamide)