title: Inhibitory effect of ibudilast (KC-404) on the expression of the beta2 integrin family on an eosinophilic cell line (EoL-1).
abstract: BACKGROUND
  Adhesion molecules are thought to play a key role in inflammatory processes in bronchial asthma. We previously observed an increased expression of the beta2-integrin family on an eosinophilic cell line (EoL-1) by platelet-activating factor (PAF).
  
  
  OBJECTIVE
  In the present study, we examined the effect of ibudilast (KC-404), a novel anti-asthma agent, on beta2 integrin expression induced by PAF.
  
  
  METHODS
  EoL-1 cells (1x10(6)/mL) were incubated in the presence or absence of 10(-6) M ibudilast (KC-404), then cells were cultured in the presence or absence of PAF (10(-7) M) for 45 minutes. Flow cytometric analysis for CD11a, CD11b, and CD18 expression was examined.
  
  
  RESULTS
  Ibudilast had an inhibitory effect on beta2 integrin expression induced by PAF [CD11a: 84.8% versus 73.1% (preincubation with ibudilast), CD11b: 35.8% versus 26.2%, CD18 74.9% versus 65.6%].
  
  
  CONCLUSIONS
  Ibudilast (KC-404) has anti-inflammatory activities through its inhibitory effect on the expression of adhesion molecules on eosinophils.
authors.affiliation: Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Hondo, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Chihara
authors.forename: O
authors.initials: O
authors.lastname: Urayama
authors.forename: H
authors.initials: H
authors.lastname: Kayaba
authors.forename: K
authors.initials: K
authors.lastname: Honda
authors.forename: N
authors.initials: N
authors.lastname: Saito
authors.forename: N
authors.initials: N
authors.lastname: Hayashi
authors.forename: D
authors.initials: D
authors.lastname: Kurachi
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D001249:Asthma / Q000188:drug therapy; D001993:Bronchodilator Agents / Q000494:pharmacology*; D018821:CD18 Antigens / Q000096:biosynthesis; D006801:Humans; D016023:Integrins / Q000096:biosynthesis* / Q000187:drug effects; D016169:Lymphocyte Function-Associated Antigen-1 / Q000096:biosynthesis; D016177:Macrophage-1 Antigen / Q000096:biosynthesis; D010972:Platelet Activating Factor / Q000494:pharmacology; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000378:metabolism; D007212:Indolizines; D011720:Pyrazoles
chemical_list: D001993:Bronchodilator Agents; D018821:CD18 Antigens; D016023:Integrins; D016169:Lymphocyte Function-Associated Antigen-1; D016177:Macrophage-1 Antigen; D010972:Platelet Activating Factor; D010975:Platelet Aggregation Inhibitors; D011725:Pyridines; C038366:ibudilast; D007212:Indolizines; D011720:Pyrazoles