Identification of Cannabis pollens using an allergic patient's immunoglobulin E and purification and characterization of allergens in Cannabis pollens.
Cannabis pollen allergens were detected using the serum of an allergic patient. The allergens were then purified by sequential column chromatography (including DE52 cellulose and phenyl-Sepharose CL-4B) and preparative HPLC. The molecular weight of the allergens were determined as 10,050 and 13,706 by matrix-assisted laser desorption/ionization time of flight mass spectrometry. We utilised Western blotting and development of an enzyme-linked immunosorbent assay for the detection of Cannabis pollen allergens.
Tanaka
H
H
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Degawa
M
M
Kawata
E
E
Hayashi
J
J
Shoyama
Y
Y
D000485:Allergens / Q000032:analysis* / Q000302:isolation & purification*; D015153:Blotting, Western; D002188:Cannabis / Q000737:chemistry* / Q000276:immunology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D008970:Molecular Weight; D011058:Pollen / Q000737:chemistry* / Q000276:immunology; D012189:Retrospective Studies
D000485:Allergens; D007073:Immunoglobulin E