title: Analysis of genetic relationships and antimicrobial susceptibility of verotoxin-producing Escherichia coli strains isolated in Nagasaki Prefecture, Japan in 1996.
abstract: A total of 19 Escherichia coli O157 isolates were obtained in Nagasaki Prefecture, in the south-western part of Japan, between 1990 and 1996. Pulsed-field gel electrophoresis (PFGE) and computer-assisted analysis were applied to determine genetic relationships among these strains. Fragment patterns of the isolates in Nagasaki, as determined by PFGE, were compared with those of isolates in other areas where large outbreaks and sporadic cases of E. coli O157 infection occurred. Similarity values of all the strains isolated in Nagasaki Prefecture were over 0.65 except for E. coli O26. Some strains were identical to the strains isolated from the areas where large outbreaks occurred. All strains were susceptible to ampicillin, fosfomycin, minocycline, amikacin, ofloxacin and sulfamethoxazole-trimethoprim.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University School of Medicine, Nagasaki, Japan. peaton-ngs@umin.ac.jp
authors.forename: K
authors.initials: K
authors.lastname: Izumikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Hirakata
authors.forename: T
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: R
authors.initials: R
authors.lastname: Yoshida
authors.forename: M
authors.initials: M
authors.lastname: Nakano
authors.forename: J
authors.initials: J
authors.lastname: Matsuda
authors.forename: C
authors.initials: C
authors.lastname: Mochida
authors.forename: S
authors.initials: S
authors.lastname: Maesaki
authors.forename: K
authors.initials: K
authors.lastname: Tomono
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
authors.forename: T
authors.initials: T
authors.lastname: Tashiro
authors.forename: S
authors.initials: S
authors.lastname: Kohno
authors.forename: S
authors.initials: S
authors.lastname: Kamihira
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001427:Bacterial Toxins; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D019453:Escherichia coli O157 / Q000145:classification / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests; D022622:Shiga Toxin 1
chemical_list: D000900:Anti-Bacterial Agents; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D022622:Shiga Toxin 1