title: Osteopontin and bone sialoprotein distribution at the bone graft recipient site.
abstract: BACKGROUND
  The area in close proximity to a bone graft is subject to marked remodeling activity, which may dramatically affect graft survival.
  
  
  OBJECTIVE
  To specifically analyze the effects at the recipient bed-onlay graft interface.
  
  
  METHODS
  In 22 adult Lewis rats, bicortical grafts were positioned below the temporal muscle and subperiosteally over the parietal bone. The recipient bone was left intact or ground to remove the external cortical layer, thereby exposing the graft to the osteopotent cells of the bone marrow. The rats were killed after 4 or 20 weeks. The outcome was assessed by routine histologic examination and immunohistochemical labeling for 2 bone matrix proteins, osteopontin and bone sialoprotein, which are involved in bone resorption and formation, respectively.
  
  
  RESULTS
  Placement of the grafts submuscularly or grinding of the outer cortical layer of the host bed increased recipient site resorption. Resorptive activity (labeling) was concentrated to a subzone below the surface of the recipient bone; neither the graft surface nor the interface soft tissues were labeled.
  
  
  CONCLUSIONS
  The successive loss of skeletal contour after bone grafting, in many cases, may largely result from recipient site failure rather than graft size reduction.
authors.affiliation: Department of Plastic Surgery, University Hospital MAS, Malmö, Sweden.
authors.forename: P
authors.initials: P
authors.lastname: Alberius
authors.forename: M
authors.initials: M
authors.lastname: Gordh
mesh_terms: D000818:Animals; D016723:Bone Remodeling / Q000502:physiology*; D016025:Bone Transplantation / Q000379:methods*; D001842:Bone and Bones / Q000473:pathology; D006085:Graft Survival; D008297:Male; D016348:Osseointegration; D053495:Osteopontin; D051381:Rats; D011917:Rats, Inbred Lew; D012795:Sialoglycoproteins / Q000032:analysis*
chemical_list: D012795:Sialoglycoproteins; C506897:Spp1 protein, rat; D053495:Osteopontin