title: Production of temperature-sensitive clones of Mycoplasma synoviae for evaluation as live vaccines.
abstract: An Australian field isolate of Mycoplasma synoviae (MS), 89079/7NS, was exposed to the mutagen N-nitro-N'-methyl-N-nitrosoguanidine. Fifteen clones from the exposed culture were characterized for temperature sensitivity. Four clones labelled B, D, G, and H were temperature sensitive and were further characterized for their ability to colonize chickens and elicit an immune response. Serum antibody responses to MS were detected 3 wk after infection, by eyedrop, in 10 of 10 birds inoculated with 86079/7NS and clones B and G and in 9 of 10 birds inoculated with clone H. No MS antibody response was observed in any bird inoculated with clone D. MS was recovered from the upper trachea of 10 of 10 birds inoculated with clones B, G, and H at 2 wk after infection. No MS was isolated from birds inoculated with clone D. Clone H, designated MS-H, was selected as a potential vaccine candidate.
authors.affiliation: School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
authors.forename: C J
authors.initials: CJ
authors.lastname: Morrow
authors.forename: J F
authors.initials: JF
authors.lastname: Markham
authors.forename: K G
authors.initials: KG
authors.lastname: Whithear
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines; D002645:Chickens; D005260:Female; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009174:Mycoplasma / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000276:immunology*; D009175:Mycoplasma Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D011201:Poultry Diseases / Q000276:immunology* / Q000517:prevention & control; D013696:Temperature; D014132:Trachea / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D009153:Mutagens; D008769:Methylnitronitrosoguanidine