Polymerase chain reaction-restriction fragment length polymorphism analysis of a short fragment of the cytochrome b gene for identification of flatfish species.
Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). PCR amplification of the cytochrome b gene using a universal primer together with a primer specifically designed as a part of this study produced a 201-bp fragment in all species analyzed. Digestions of the PCR products with Sau3Al, BsmAl, Rsal, and Mn/l endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.
Céspedes
A
A
Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
García
T
T
Carrera
E
E
González
I
I
Sanz
B
B
Hernández
P E
PE
Martín
R
R
D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis; D005412:Flatfishes / Q000145:classification* / Q000235:genetics; D005513:Food Inspection / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013045:Species Specificity
D003573:Cytochrome b Group; D004272:DNA, Mitochondrial