Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay.
We developed a monoclonal antibody-based, antigen capture sandwich enzyme-linked immunosorbent assay (ELISA) for bovine coronavirus. We compared the ELISA with electron microscopy and the hemagglutination test and found a close correlation between them. The sensitivity of the ELISA was 10(4) bovine coronavirus particles per ml of 10% fecal suspension. Compared with electron microscopy, bovine coronavirus ELISA had 96% specificity.
Schoenthaler
S L
SL
Department of Diagnostic Medicine-Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas 66506-5606, USA.
Kapil
S
S
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis / Q000821:virology; D018352:Coronavirus Infections / Q000175:diagnosis / Q000662:veterinary / Q000821:virology; D017938:Coronavirus, Bovine / Q000276:immunology* / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000706:statistics & numerical data / Q000662:veterinary*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000821:virology; D006386:Hemagglutination Tests; D008854:Microscopy, Electron; D012680:Sensitivity and Specificity
D000956:Antigens, Viral