title: Comparison of two PCRs for detection of Mycobacterium ulcerans.
abstract: Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Guimaraes-Peres
authors.forename: F
authors.initials: F
authors.lastname: Portaels
authors.forename: P
authors.initials: P
authors.lastname: de Rijk
authors.forename: K
authors.initials: K
authors.lastname: Fissette
authors.forename: S R
authors.initials: SR
authors.lastname: Pattyn
authors.forename: J
authors.initials: J
authors.lastname: van Vooren
authors.forename: P
authors.initials: P
authors.lastname: Fonteyne
mesh_terms: D001431:Bacteriological Techniques; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D019911:Mycobacterium ulcerans / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S