title: Induction of antigen presentation by macrophages after phagocytosis of tumour apoptotic cells.
abstract: Due to their resistance to classical chemotherapies, most human colorectal cancers have a high incidence and a poor prognosis. Immunotherapy using interleukin 2 (IL2) has provided disappointing results in the treatment of these cancers. Recently, however, we have demonstrated that a treatment combining a cell-differentiating agent, sodium butyrate (NaBut) with IL2 resulted in a remission of established peritoneal colorectal carcinomatosis in rats. Separately, neither NaBut nor IL2 treatment cured these tumour-bearing rats. NaBut is known to induce cell differentiation and subsequent apoptosis in epithelial cells, while IL2 stimulates the immune cells capable of participating in tumour rejection. We postulated that the significant therapeutic effect of NaBut/IL2 treatment could be attributed to a NaBut-induced increase in the immunogenicity of the cancer cells. We report here that NaBut induced an apoptotic process in rat colon tumour cells in vivo and in vitro. We observed, in an efficient cure, colocalization of apoptotic bodies and monocytes/macrophages at the periphery of the tumour. We propose that these apoptotic bodies are phagocytosed in vivo by the macrophages. We also showed in vitro that a subpopulation of macrophages involved in the phagocytic clearance of apoptotic cells expresses cell surface molecules associated with antigen presentation and stimulates the proliferation of naive splenocytes. Our data suggest that therapies that recruit massive induction of the apoptotic process in tumour cells could favour tumour antigen presentation via their specific phagocytosis by antigen-presenting cells (APCs). We propose that the development of specific therapies that stimulate both tumour cell apoptosis and the immune system could offer new opportunities in anti-cancer treatments of poorly immunogenic cancer cells.
authors.affiliation: INSERM U419, Institute of Biology, Nantes, France.
authors.forename: F
authors.initials: F
authors.lastname: Henry
authors.forename: L
authors.initials: L
authors.lastname: Bretaudeau
authors.forename: I
authors.initials: I
authors.lastname: Barbieux
authors.forename: K
authors.initials: K
authors.lastname: Meflah
authors.forename: M
authors.initials: M
authors.lastname: Gregoire
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D002087:Butyrates / Q000494:pharmacology; D002455:Cell Division; D015179:Colorectal Neoplasms / Q000276:immunology*; D006801:Humans; D008264:Macrophages / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology*; D051381:Rats; D013154:Spleen / Q000166:cytology; D014407:Tumor Cells, Cultured
chemical_list: D002087:Butyrates