Establishment and characterization of a hypocatalasemic mouse cell strain.
Contact-inhibited catalase-deficient fibroblast cell strain has been established from the homozygous hypocatalasemic C3H/Csb mutant mouse. This cell strain has low level of catalase enzyme activity and has normal level of enzyme activities of both glutathione peroxidase and superoxide dismutase. Catalase-deficient C3H/Csb mutant cell strain is markedly more sensitive to the toxicity of hydrogen peroxide compared to wild-type C3H/Csa cell strain. In addition, mutant cell strain is sensitive to X-rays and near-UV compared to wild-type cell strain, but shows the same sensitivities to topoisomerase II inhibitors, adriamycin and 4'-(9-acridinylamino) methanesulfon-m-anisidide (m-AMSA), and the DNA cross-linking agents, cisdiamminedichloroplatinum (II) (cis-Pt) and trans-diamminedichloroplatinum (II) (trans-Pt). These cell strains will be of use in the study of the roles which catalase plays in the intracellular prevention of DNA damage induced by oxidative stress.
Utsumi
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H
Laboratory of Radiation Life Science, Kyoto University, Osaka, Japan.
Kodama
S
S
Tano
K
K
Hashimoto
M W
MW
Watanabe
H
H
D020642:Acatalasia; D000818:Animals; D002374:Catalase / Q000235:genetics*; D002460:Cell Line; D005260:Female; D005347:Fibroblasts / Q000201:enzymology / Q000528:radiation effects; D006861:Hydrogen Peroxide / Q000633:toxicity; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008817:Mice, Mutant Strains; D011836:Radiation Tolerance / Q000235:genetics / Q000502:physiology; D013696:Temperature; D014466:Ultraviolet Rays / Q000009:adverse effects
D006861:Hydrogen Peroxide; D002374:Catalase