A stable reagent system for screening and identifying red blood cell irregular antibodies: application to commercial antibodies.
OBJECTIVE
Development of a new solid-phase system for screening and identifying irregular red cell antibodies.


METHODS
Red blood cell membranes were prepared by a semi-automated procedure in which the hemolysate solution was passed through a hollow-fiber system. The membranes were fixed to the solid phase (microtiter plates) by centrifugation and incubated with 8% fat-free milk. Antibodies added to the microtiter plate were detected by anti-human antibodies adsorbed onto yellow latex particles.


RESULTS
The system had good sensitivity (titer <1); 97% of anti-D samples were detected. The detection system was stable for 6 months at 4 degrees C.


CONCLUSIONS
This stable-antigen solid-phase system readily detects and identifies red cell antibodies that are important in transfusion.
Million
L
L
Laboratoire d'Hématologie-Physiologie, Faculté de Pharmacie, Nancy, France.
Pellerin
C
C
Marchand-Arvier
M
M
Vigneron
C
C
D003132:Commerce; D004355:Drug Stability; D004910:Erythrocyte Membrane / Q000276:immunology*; D006801:Humans; D007202:Indicators and Reagents; D007518:Isoantibodies / Q000097:blood*; D007519:Isoantigens / Q000097:blood*; D008403:Mass Screening / Q000379:methods*; D010316:Particle Size; D018029:Rho(D) Immune Globulin / Q000276:immunology*
D007202:Indicators and Reagents; D007518:Isoantibodies; D007519:Isoantigens; D018029:Rho(D) Immune Globulin