title: The c-wave of the electroretinogram recorded under clinical conditions from rabbits.
abstract: To determine whether large and repeatable c-waves can be recorded from rabbits with equipment already in use in clinical electroretinographic laboratories, the Burian-Allen electrode, connected bipolarly or monopolarly, was used to record electroretinograms from pigmented rabbits. The Jet electrode was also used. The c-waves elicited by long-duration (4-second) stimuli were compared to those elicited by stroboscopic stimuli. In addition, the c-waves recorded with direct-coupled amplification were compared to those recorded with condenser-coupled amplification (one-half-amplitude bandpass=0.1 Hz). The b-wave amplitude was not altered by the amplifier coupling or by the two stimulus durations. The largest c-waves were elicited by 4-second-duration stimuli and recorded with direct-coupled amplification. Although the c-wave amplitude was reduced by stroboscopic stimuli and by condenser coupling, large and repeatable c-waves were elicited by stroboscopic stimuli and recorded with condenser-coupled amplification. A comparison of stimulus duration and amplifier coupling showed that the stimulus duration was more important in recording large-amplitude c-waves. Similar results were obtained with the Jet electrode. We conclude that repeatable and large c-waves can be elicited by a stroboscopic stimuli and can be recorded with condenser-coupled amplification with good low-frequency response from rabbits.
authors.affiliation: William McKnight Vision Research Center, Bascom Palmer Eye Institute, University of Miami, School of Medicine, FL 33136, USA.
authors.forename: D I
authors.initials: DI
authors.lastname: Hamasaki
authors.forename: K
authors.initials: K
authors.lastname: Korabathina
authors.forename: S R
authors.initials: SR
authors.lastname: Patel
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: B L
authors.initials: BL
authors.lastname: Lam
mesh_terms: D000818:Animals; D003623:Dark Adaptation; D004596:Electroretinography / Q000295:instrumentation / Q000379:methods*; D008839:Microelectrodes; D010775:Photic Stimulation; D010857:Pigment Epithelium of Eye / Q000502:physiology; D011817:Rabbits; D012160:Retina / Q000502:physiology*