title: Determination of Hb F levels: the routine methods.
abstract: The two classical methods used to determine the level of Hb F in a hemolysate are herein described. Measurement of this hemoglobin fraction by resistance to alkali denaturation was the first technique introduced; it is today largely replaced by ion exchange high performance liquid chromatography. The limits, pitfalls, and advantages of the two methods are discussed.
authors.affiliation: Service de Biochimie, Hôpital Henri Mondor, Créteil, France.
authors.forename: C
authors.initials: C
authors.lastname: Préhu
authors.forename: R
authors.initials: R
authors.lastname: Ducrocq
authors.forename: C
authors.initials: C
authors.lastname: Godart
authors.forename: J
authors.initials: J
authors.lastname: Riou
authors.forename: F
authors.initials: F
authors.lastname: Galactéros
mesh_terms: D000468:Alkalies / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005319:Fetal Hemoglobin / Q000032:analysis* / Q000276:immunology; D006452:Hemoglobinometry / Q000379:methods*; D006801:Humans
chemical_list: D000468:Alkalies; D005319:Fetal Hemoglobin