Coupled column chromatography in chiral separation of salmeterol.
A coupled achiral-chiral high-performance liquid chromatographic system has been developed for the determination of the enantiomers of salmeterol, S-(+)-salmeterol and R-(-)-salmeterol in urine. The salmeterol was separated from the interfering components in urine and quantified on the silica column, and the enantiomeric composition was determined on a Sumichiral OA-4700 chiral stationary phase. The two columns were connected by a switching valve equipped with a silica precolumn. The precolumn was used to concentrate the salmeterol in the eluent from the achiral column before backflushing onto the chiral phase. The coupled system was validated.
Kim
K H
KH
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Yun
H W
HW
Kim
H J
HJ
Park
H J
HJ
Choi
P W
PW
D000318:Adrenergic beta-Agonists / Q000302:isolation & purification* / Q000652:urine; D000420:Albuterol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000652:urine; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D006801:Humans; D000068299:Salmeterol Xinafoate; D013237:Stereoisomerism
D000318:Adrenergic beta-Agonists; D000068299:Salmeterol Xinafoate; D000420:Albuterol