On-line polymerase chain reaction (PCR) monitoring.
In this short note, we present the results of a case study for monitoring the whole polymerase chain reaction (PCR) process (all steps) with a glass fiber fluorometer that was described in a former publication. To utilize this fluorometer, which was originally constructed for a PCR machine with three thermostating devices, a new thermostating device has been developed: the glass fiber matrix is integrated into the thermostating device, while the PCR samples are heated and cooled. The device is able to monitor all samples throughout all stages of PCR with the help of an intercalating dye. This approach also permits one to choose arbitrarily different cooling and heating rates.
Ederhof
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Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany.
Walter
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NG
Schober
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D005470:Fluorometry / Q000379:methods; D016133:Polymerase Chain Reaction / Q000295:instrumentation* / Q000379:methods*; D012984:Software; D013696:Temperature