title: Differential expression of peroxisome proliferator-activated receptor subtypes during the differentiation of human keratinocytes.
abstract: The expression of mRNA encoding peroxisome proliferator-activated receptor (PPAR) subtypes in human keratinocytes was determined by semiquantitative reverse transcriptase-polymerase chain reaction. When normal human keratinocytes were induced to differentiate by shifting the culture medium to high Ca2+ concentration, the expression of PPAR-alpha and -gamma mRNA was increased, whereas that of PPAR-delta remained unchanged. At the protein level, the expression of PPAR in cultured human keratinocytes was demonstrated by a DNA mobility shift assay and the functionality of the receptor subtypes was assessed by transactivation experiments. In epidermis reconstructed in vitro, the level of PPAR-alpha and -gamma mRNA was also associated with keratinocyte differentiation. In lesional compared with nonlesional psoriatic epidermis, the expression of PPAR-alpha and -gamma mRNA was reduced, indicating that these two subtypes are tightly linked to the epidermal differentiation process.
authors.affiliation: CIRD GALDERMA, Sophia Antipolis, Valbonne, France.
authors.forename: M
authors.initials: M
authors.lastname: Rivier
authors.forename: I
authors.initials: I
authors.lastname: Safonova
authors.forename: P
authors.initials: P
authors.lastname: Lebrun
authors.forename: C E
authors.initials: CE
authors.lastname: Griffiths
authors.forename: G
authors.initials: G
authors.lastname: Ailhaud
authors.forename: S
authors.initials: S
authors.lastname: Michel
mesh_terms: D002454:Cell Differentiation / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D015603:Keratinocytes / Q000737:chemistry / Q000166:cytology*; D011565:Psoriasis / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000378:metabolism; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation
chemical_list: D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors