title: Ultrastructural localisation of spectrin in sensory and supporting cells of guinea-pig organ of Corti.
abstract: Spectrin is a cytoskeletal protein found in the cortex of many cell types. It is known to occur in cochlear outer hair cells (OHCs) with previous immunoelectron microscopical studies showing that it is located in the cuticular plate and the cortical lattice. The latter is a network of filaments associated with the lateral plasma membrane that is thought to play a role in OHC motility. Spectrin has also been found in inner hair cells (IHCs) and supporting cells using immunofluorescent techniques, but its ultrastructural distribution in these cells has not yet been described. This has, therefore, been investigated using a monoclonal antibody to alpha-spectrin in conjunction with pre- and post-embedding immunogold labelling for transmission electron microscopy. Labelling was found in a meshwork of filaments beneath the plasma membranes of both IHCs and supporting cells and, in pillar cells, close to microtubule/microfilament arrays. It was also found in association with the stereocilia of OHCs and IHCs and, as expected, in the cortical lattice and cuticular plate of OHCs. Thus, spectrin is a general component of cytoskeletal structures involved in maintaining the specialised cell shapes in the organ of Corti and may contribute to the mechanical properties of all the cell types examined.
authors.affiliation: Department of Communication and Neuroscience, Keele University, Staffs, UK. coa03@keele.ac.uk
authors.forename: S
authors.initials: S
authors.lastname: Mahendrasingam
authors.forename: D N
authors.initials: DN
authors.lastname: Furness
authors.forename: C M
authors.initials: CM
authors.lastname: Hackney
mesh_terms: D000818:Animals; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000378:metabolism* / Q000648:ultrastructure*; D015151:Immunoblotting; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009925:Organ of Corti / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure*; D013049:Spectrin / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D013049:Spectrin