[A new method for evaluation of the antagonistic action of bacterial lactic acid (LAB) on selected pathogenic indicator bacteria].
A comparison was made of three methods: paper disc, double layer and a newly proposed agar slab techniques for testing antibacterial activity of Lactobacillus strains. Strains of indicator bacteria were selected from important pathogens of the human alimentary and genitourinary tract. The agar slab method, which is based on applying of slabs of MRS agar with overnight growth of the antagonistic bacteria to the surface of appropriate agar plates seeded with indicator bacteria, appeared to be the only method suitable for testing of aerobic and anaerobic fastidious bacteria. After testing of 27 Lactobacillus strains against 3 selected indicator bacteria: E. coli, Salmonella enteritidis and Staphylococcus aureus, calculating mean values and standard deviations for diameters of inhibitory zones and making variance analysis it was shown that in comparison to other methods the agar slab technique, although less sensitive than others, gave most consistent and reproducible results.
Strus
M
M
Zakład Bakteriologii Instytutu Mikrobiologii Collegium Medicum Uniwersytetu Jagiellońskiego.
D004064:Digestive System / Q000382:microbiology; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000378:metabolism*; D007778:Lactobacillus / Q000378:metabolism*; D008826:Microbial Sensitivity Tests / Q000379:methods*; D015203:Reproducibility of Results; D013045:Species Specificity; D013499:Surface Properties; D014566:Urogenital System / Q000382:microbiology
D019344:Lactic Acid
Nowa metoda oceny antagonistycznego działania bakterii kwasu mlekowego (LAB) na wybrane, chorobotwórcze bakterie wskaźnikowe.