title: Lack of humoral and cellular responses against glutathione S-transferases in Fasciola hepatica experimentally infected sheep.
abstract: Anti-Fasciola hepatica excretory-secretory products (FhESP) and anti-glutathione S-transferase (GST) immune response were studied in sheep infected with 250 F. hepatica metacercariae. In FhESP, 29 proteins with molecular masses (m) of 10-200 kDa were identified by SDS-PAGE. GST m were 26.7 and 27.2 kDa. The anti-FhESP humoral response was precocious and increased throughout the infection. FhESP contains several immunoreactive antigens but no anti-GST antibodies were detected either by ELISA or western blotting. The anti-FhESP cellular response increased precociously and fell after the second week of infection but no cellular response against GST was observed.
authors.affiliation: Ecole nationale vétérinaire de Nantes, France. moreau@vet-nantes.fr
authors.forename: E
authors.initials: E
authors.lastname: Moreau
authors.forename: A
authors.initials: A
authors.lastname: Chauvin
authors.forename: C
authors.initials: C
authors.lastname: Boulard
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000917:Antibody Formation; D004797:Enzyme-Linked Immunosorbent Assay; D005210:Fasciola hepatica / Q000201:enzymology* / Q000276:immunology; D005211:Fascioliasis / Q000276:immunology / Q000662:veterinary*; D005982:Glutathione Transferase / Q000276:immunology*; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000097:blood*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000469:parasitology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D005982:Glutathione Transferase