Isolation and characterisation of the acetyl-CoA carboxylase gene from Aspergillus nidulans.
The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequence homology to acetyl-CoA carboxylases from other organisms, putative biotin-, ATP-, HCO3-- and acetyl-CoA- binding sites have been assigned. Northern data and ACC enzyme-activity measurements from A. nidulans suggested that expression of accA was higher in media containing nitrate than ammonia as a sole nitrogen source. Deletion of accA in A. nidulans was unsuccessful. The failure of A. nidulans to grow in the presence of the ACC-specific inhibitor, soraphen A, supplemented with C16-18 fatty acids suggested that ACC is an essential enzyme.
Morrice
J
J
Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, Norfolk, NR4 7UA, UK.
MacKenzie
D A
DA
Parr
A J
AJ
Archer
D B
DB
D000103:Acetyl-CoA Carboxylase / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000166:cytology / Q000201:enzymology / Q000235:genetics*; D001483:Base Sequence; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000737:chemistry; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005800:Genes, Fungal / Q000235:genetics*; D008969:Molecular Sequence Data
D003470:Culture Media; D017077:Culture Media, Conditioned; D004271:DNA, Fungal; D000103:Acetyl-CoA Carboxylase