title: Functional analysis of the C(-188)A polymorphism of the human leptin promoter.
abstract: Mutational analysis of the promoter region of the leptin gene in morbidly obese Finnish subjects had revealed a previously unidentified C(-188)A polymorphism in the proximal promoter that showed a weak association with elevated serum leptin levels in obese male carriers of the variant (-188A) allele. In this study we demonstrated that neither expression of reporter gene constructs driven by wild-type (-188C) or variant (-188A) proximal promoter regions, nor assay of binding of cellular proteins reveal a genotype-related difference in promoter activity.
authors.affiliation: Department of Medicine, Institute of Biomedicine, University of Helsinki, Finland.
authors.forename: L
authors.initials: L
authors.lastname: Oksanen
authors.forename: J J
authors.initials: JJ
authors.lastname: Palvimo
authors.forename: O A
authors.initials: OA
authors.lastname: Jänne
authors.forename: K
authors.initials: K
authors.lastname: Kontula
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes; D000818:Animals; D001483:Base Sequence; D017930:Genes, Reporter; D020738:Leptin; D008156:Luciferases / Q000235:genetics; D051379:Mice; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000235:genetics*
chemical_list: D020738:Leptin; D011506:Proteins; D008156:Luciferases