A method for endoscopic embryo collection and transfer in the rabbit.
Thirty-two rabbits were used for endoscopic embryo collection and transfer. For embryo collection midventral laparoscopy and transcervical endoscopy were combined for orthograd flushing of the oviducts and uterine horns. Transfer was performed transcervically under laparoscopic control. The mean number of corpora lutea counted in nine donors was 13.3 +/- 8.4. A total of 72 morulae/blastocysts were obtained. No embryos were recovered when only the uterine horns were flushed. 291 embryos were transcervically transferred to 23 recipients which resulted in 10 pregnant animals at day 12. Two of three slaughtered recipients showed together 4 implantation sites. Four animals delivered 12 pups.
Besenfelder
U
U
Institute of Animal Breeding and Genetics, University of Veterinary Sciences, Vienna, Austria. besenfel@ifa-tulln.ac.at
Strouhal
C
C
Brem
G
G
D000818:Animals; D001755:Blastocyst; D010064:Embryo Implantation; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D004724:Endoscopy; D005187:Fallopian Tubes; D005260:Female; D009028:Morula; D011247:Pregnancy; D011817:Rabbits; D012099:Reproductive Techniques / Q000662:veterinary*; D014599:Uterus