title: Simultaneous determination of pseudoephidrine HCl (PSE) and terfanidine (TER) from formulations by reversed-phase ion pair high-performance liquid chromatography (RP-HPLC).
abstract: A new simple, precise, rapid, and selective reversed-phase ion pair high-performance liquid chromatography (RP-HPLC) method has been developed for the simultaneous determination of pseudoephidrine (PSE) and terfanidine (TER) from tablets using 60:15:25 acetontrile:methanol:water (v/v) containing 2.9 g sodium lauryl sulfate/liter, pH adjusted to 3.1 using phosphoric acid as a mobile phase and C18 Spherisorb ODS 2 (3 microns, 5 cm x 4.6 mm i.d.) as stationary phase. Detection was carried out using a UV detector at 254 nm. A constant flow of 1.0 ml/min was maintained throughout the analysis. Retention times for PSE and TER were 1.90 and 7.35 min, respectively. Linearity range and percentage recoveries for PSE and TER were 24-1200 and 12-600 micrograms/ml, and 100.01 and 100.4%, respectively.
authors.affiliation: Department of Chemistry, Institute of Science, Mumbai, India.
authors.forename: A P
authors.initials: AP
authors.lastname: Argekar
authors.forename: S J
authors.initials: SJ
authors.lastname: Shah
authors.forename: S V
authors.initials: SV
authors.lastname: Raj
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000032:analysis*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D013607:Tablets; D016593:Terfenadine / Q000032:analysis*
chemical_list: D012996:Solutions; D013607:Tablets; D016593:Terfenadine; D004809:Ephedrine