Evaluation of a mucoadhesive buccal patch for delivery of peptides: in vitro screening of bioadhesion.
We have assessed the bioadhesive properties of several different mucoadhesive buccal patches. The patches consisted of custom coformulations of silicone polymers and Carbopol 974P. The contact angle of water was measured for each of the test formulations, using an ophthalmic shadow scope. The corresponding work of adhesion between the water and the patches (W1), and between the patches and freshly-excised rabbit buccal mucosa (W2) was then calculated, using a modification of Dupre's equation. The bioadhesive strength between the patches and excised rabbit buccal mucosa was also assessed. The results of the contact-angle measurements indicated that the contact angle decreased with an increase in the amount of Carbopol in the formulation. Additionally, the calculated values of both W1 and W2 increased with an increase in the amount of Carbopol in the buccal-patch formulations. A correlation (r not equal to 0.9808) was found between the measured contact angle and the calculated values for W2. The direct measurement of the force required to separate a buccal patch from excised rabbit buccal mucosa with the INSTRON demonstrated that the adhesive strength increased with an increase in the amount of Carbopol. This preliminary study has shown that the measurement of contact angles alone may provide a useful technique for estimating the work of adhesion, and may serve as a convenient and rapid screening procedure to identify potential mucoadhesive buccal-patch formulations.
Li
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Division of Pharmaceutics and Pharmacodynamics, College of Pharmacy, University of Illinois, Chicago 60612, USA.
Bhatt
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Johnston
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D000180:Acrylic Resins / Q000737:chemistry; D000268:Adhesiveness; D000279:Administration, Cutaneous; D000818:Animals; D002610:Cheek; D016503:Drug Delivery Systems; D016014:Linear Models; D008403:Mass Screening; D009061:Mouth Mucosa / Q000378:metabolism*; D010455:Peptides / Q000493:pharmacokinetics*; D011817:Rabbits
D000180:Acrylic Resins; D010455:Peptides; C006903:carbopol 940