title: Identification of proteins whose amounts are altered by mutation in the pgsA gene of Escherichia coli.
abstract: We identified proteins whose amounts were altered in an Escherichia coli pgsA3 mutant lacking the potential to synthesize phosphatidylglycerolphosphate, a precursor of phosphatidylglycerol. Proteins whose amounts were increased in the mutant were protease Do, periplasmic oligopeptide-binding protein, tryptophanase, and an unidentified protein, while the decreased one was flagellin. Transformation of the mutant with a plasmid containing the wild type pgsA gene complemented the phenotype, indicating that the pgsA3 mutation is responsible for the phenotype.
authors.affiliation: Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: A
authors.initials: A
authors.lastname: Ohba
authors.forename: R
authors.initials: R
authors.lastname: Inoue
authors.forename: N
authors.initials: N
authors.lastname: Akimitsu
authors.forename: T
authors.initials: T
authors.lastname: Mizushima
authors.forename: K
authors.initials: K
authors.lastname: Sekimizu
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005408:Flagellin / Q000378:metabolism; D006360:Heat-Shock Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D033903:Periplasmic Proteins; D017421:Sequence Analysis; D012697:Serine Endopeptidases / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics* / Q000378:metabolism; D014368:Tryptophanase / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D033903:Periplasmic Proteins; D005408:Flagellin; D017855:Transferases (Other Substituted Phosphate Groups); C020676:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase; C061682:DegP protease; D012697:Serine Endopeptidases; D014368:Tryptophanase