Column liquid chromatography determination of vitamins A and E in powdered milk and local flour: a validation procedure.
A high-performance liquid chromatography method was developed for the simultaneous routine determination of vitamins A and E in powdered milk and flour made from local plants and purchased from open markets in the Ivory Coast. The method involves saponification followed by extraction with a mixture of organic solvents. The vitamins were resolved by reversed-phase HPLC and detection at a single wavelength. The main tests of method validation were applied to the procedure. The results show the reliability of the analytical method for the intended application.
Ake
M
M
Laboratoire de Chimie Analytique, Faculté de Pharmacie, Montpellier, France.
Fabre
H
H
Malan
A K
AK
Mandrou
B
B
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007560:Cote d'Ivoire; D005433:Flour / Q000032:analysis*; D005519:Food Preservation; D008892:Milk / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins; D012997:Solvents; D014801:Vitamin A / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D012503:Saponins; D012997:Solvents; D014801:Vitamin A; D014810:Vitamin E