title: Set-up and validation of an adsorptive stripping voltammetric method for kynurenic acid determination in human urine.
abstract: Validation of an adsorptive stripping voltammetric method for kynurenic acid determination in urine, was presented. The selection of appropriate validation parameters, the design consideration for evaluation and the problem of endogenous metabolites were discussed. The considered fundamental criteria for assessing the reliability and overall performance of the method in the urine matrix were selectivity, linearity and range, limit of quantitation, accuracy, precision and analyte stability. The intermediate precision was also evaluated by means of a full factorial design. An HPLC method with fluorimetric detection was used as a reference method to assess the accuracy. The analysis in urine required a pH control as pointed out by robustness testing and the found kynurenic acid concentration in daily urine ranged from 5 to 40 microM.
authors.affiliation: Department of Pharmaceutical Sciences, University of Florence, Firenze, Italy. smr@farmfi.scifarm.unifi.it
authors.forename: S
authors.initials: S
authors.lastname: Furlanetto
authors.forename: C
authors.initials: C
authors.lastname: Tognini
authors.forename: R
authors.initials: R
authors.lastname: Carpenedo
authors.forename: E
authors.initials: E
authors.lastname: La Porta
authors.forename: S
authors.initials: S
authors.lastname: Pinzauti
mesh_terms: D000327:Adsorption; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007736:Kynurenic Acid / Q000378:metabolism / Q000652:urine*; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D007736:Kynurenic Acid