Campylobacter jejuni-stimulated secretion of interleukin-8 by INT407 cells.
Incubation of INT407 cells with various clinical isolates of Campylobacter jejuni resulted in secretion of interleukin-8 (IL-8) at levels ranging from 96 to 554 pg/ml at 24 h. The strains which produced the highest levels of IL-8 secretion were 81-176 and BT44. Induction of IL-8 secretion required live cells of 81-176 and was dependent on de novo protein synthesis. Site-specific mutants of 81-176, which were previously shown to be defective in adherence and invasion, resulted in reduced levels of secretion of IL-8, and cheY mutants of strains 81-176 and 749, which are hyperadherent and hyperinvasive, resulted in higher levels of IL-8 secretion. Another mutant of 81-176, which adheres at about 43% of the wild-type levels but is noninvasive, also showed marked reduction in IL-8 levels, suggesting that invasion is necessary for high levels of IL-8 secretion. When gentamicin was added to INT407 cells at 2 h after infection with 81-176, IL-8 secretion 22 h later was equivalent to that of controls without gentamicin, suggesting that the events which trigger induction and release of IL-8 occur early in the interactions of bacteria and eukaryotic cells.
Hickey
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Enteric Diseases Program, Naval Medical Research Center, Bethesda, Maryland, USA.
Baqar
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S
Bourgeois
A L
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Ewing
C P
CP
Guerry
P
P
D001422:Bacterial Adhesion / Q000276:immunology; D016123:Campylobacter jejuni / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D002460:Cell Line; D002633:Chemotaxis / Q000276:immunology; D015169:Colony Count, Microbial; D004622:Embryo, Mammalian; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000382:microbiology*; D007700:Kinetics; D008826:Microbial Sensitivity Tests
D005839:Gentamicins; D016209:Interleukin-8