title: Autoradiographic evidence that zona glomerulosa and capsular vessels of the human adrenal cortex are provided with different subtypes of adrenomedullin receptors.
abstract: Frozen sections of normal adrenal glands, obtained from patients undergoing unilateral nephrectomy for kidney cancer, were labeled in vitro with human [125I]ADM(1-52). Autoradiography and quantitative densitometry showed the presence of abundant ADM(1-52) binding sites in both zona glomerulosa (ZG) and capsular vessels, which were displaced with about the same efficiency by cold ADM(1-52) and rat ADM(1-50). The selective calcitonin gene-related peptide type 1 (CGRPI) ligand CGRP(8-37) eliminated, although less efficiently than ADMs, [125I]ADM(1-52) binding in the ZG, but not in the capsular vessels. These findings suggest the existence of different receptor subtypes for ADM in the human adrenal cortex. The CGRP(8-37)-sensitive receptors located in the ZG may mediate the well-known inhibitory effect of ADM on aldosterone secretion, while the CGRP(8-37)-insensitive receptors present in the capsular vessel may be involved in the ADM-induced rise in adrenal blood flow.
authors.affiliation: Department of Anatomy, University of Padua, Italy.
authors.forename: A S
authors.initials: AS
authors.lastname: Belloni
authors.forename: V
authors.initials: V
authors.lastname: Meneghelli
authors.forename: H C
authors.initials: HC
authors.lastname: Champion
authors.forename: W A
authors.initials: WA
authors.lastname: Murray
authors.forename: D H
authors.initials: DH
authors.lastname: Coy
authors.forename: P J
authors.initials: PJ
authors.lastname: Kadowitz
authors.forename: G G
authors.initials: GG
authors.lastname: Nussdorfer
mesh_terms: D000302:Adrenal Cortex / Q000098:blood supply / Q000737:chemistry*; D053607:Adrenomedullin; D000328:Adult; D001345:Autoradiography; D001808:Blood Vessels / Q000737:chemistry*; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification*; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D058265:Receptors, Adrenomedullin; D018000:Receptors, Peptide; D015384:Zona Glomerulosa / Q000098:blood supply / Q000737:chemistry
chemical_list: D008565:Membrane Proteins; D010446:Peptide Fragments; D010455:Peptides; D058265:Receptors, Adrenomedullin; D018000:Receptors, Peptide; C108043:adrenomedullin (1-50); C058636:calcitonin gene-related peptide (8-37); D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide