Five homologous repeats of the protein G-related protein MIG cooperate in binding to goat immunoglobulin G.
Protein MIG, from Streptococcus dysgalactiae, binds alpha2-macroglobulin and immunoglobulin G (IgG). MIG-derived fusion proteins with one to five IgG-binding repeats differed up to 72,000- fold in avidity for goat IgG, indicating a considerable cooperativity of the repeats. Significant sequence variation in the IgG-binding repeats was recognized. Protein MIG interacted with goat IgG1 via both the Fc and Fab parts.
Vasi
J
J
Department of Microbiology, SLU, Uppsala, Sweden.
Svensson
J
J
Frick
I M
IM
Müller
H P
HP
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D054370:Chemokine CXCL9; D019743:Chemokines, CXC / Q000235:genetics / Q000378:metabolism*; D006041:Goats; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D008414:Mastitis, Bovine / Q000382:microbiology; D017452:Receptors, IgG / Q000235:genetics / Q000378:metabolism*; D011971:Receptors, Immunologic / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D020449:Repetitive Sequences, Amino Acid; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000378:metabolism*
D001426:Bacterial Proteins; C111954:CXC chemokine Mig; D054370:Chemokine CXCL9; D019743:Chemokines, CXC; C034284:IgG Fc-binding protein, Streptococcus; D026503:Low Density Lipoprotein Receptor-Related Protein-1; D017452:Receptors, IgG; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins