title: Soluble CD21 induces activation and differentiation of human monocytes through binding to membrane CD23.
abstract: Interactions between CD23, the low-affinity receptor for IgE, and CD21, the C3d/EBV receptor, modulate several intracellular events in lymphocytes. A soluble form of CD21 (sCD21) corresponding to the extracellular domain of the receptor circulates in normal plasma. We now demonstrate that purified sCD21 acts as a functional ligand for CD23-expressing monocytes. Soluble CD21 induced an increase in intracellular cGMP levels and the production of IL-6 and TNF-alpha in IL-4-pretreated monocytes induced to express CD23 but not in unstimulated CD23- monocytes. The accumulation of cGMP and the production of TNF-alpha were inhibited by NG-monomethyl-L-arginine (L-NMMA), indicating that sCD21 activates the L-arginine pathway of NO production. We demonstrated that sCD21 activates NO synthase (NOS) since it was found to enhance the conversion of L-arginine into L-citrulline and induce the intracellular expression of inducible NOS in CD23+ monocytes. In addition, sCD21 was shown to up-regulate the expression of HLA-DR and CD40 and decrease that of CD14 on cultured CD23+ monocytes. Thus, in a fashion similar to IgE complexes, sCD21 is able to efficiently trigger CD23 signaling pathways, inducing the release of pro-inflammatory mediators by human monocytes. Soluble CD21 up-regulates the expression of molecules involved in antigen presentation, further suggesting a potential immunoregulatory function for the soluble molecule.
authors.affiliation: INSERM U430, Hôpital Broussais, Paris, France. fremeaux@hbroussais.fr
authors.forename: V
authors.initials: V
authors.lastname: Frémeaux-Bacchi
authors.forename: J P
authors.initials: JP
authors.lastname: Aubry
authors.forename: J Y
authors.initials: JY
authors.lastname: Bonnefoy
authors.forename: M D
authors.initials: MD
authors.lastname: Kazatchkine
authors.forename: J P
authors.initials: JP
authors.lastname: Kolb
authors.forename: E M
authors.initials: EM
authors.lastname: Fischer
mesh_terms: D017951:Antigen Presentation; D002478:Cells, Cultured; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D017464:Receptors, Complement 3d / Q000276:immunology*; D017455:Receptors, IgE / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology; D015854:Up-Regulation
chemical_list: D017464:Receptors, Complement 3d; D017455:Receptors, IgE