title: [Blockade of (+/-) 12-chloroscoulerine on feed-back regulation of dopamine D2 autoreceptors].
abstract: OBJECTIVE
  To verify whether (+/-) 12-chloroscoulerine (CSL) is antagonist or agonist effect to D2 autoreceptors.
  
  
  METHODS
  The levodopa content accumulated in the rat striatum was measured by HPLC-ECD, and the DA neuron firing activity in the substantia nigra zona compacta (SNC) was recorded.
  
  
  RESULTS
  The accumulated levodopa content induced by CSL 40 mg.kg-1 was much more than that of 1,4-butyro-lactone (BL) group (P < 0.01). After i.p. injection of apomorphine (Apo) 5 mg.kg-1, the levodopa content was decreased below that of BL group (P < 0.05). The Apo inhibition on levodopa content was completely reversed by CSL (40 mg.kg-1, i.p.) and then increased the levodopa content (2.5 +/- 1.1 micrograms.g-1) over that of Apo group (0.7 +/- 0.3 microgram.g-1, P < 0.01). In the electrophysiologic recording, Apo (15 micrograms.kg-1, i.v.) induced the decrease of SNC DA cell firing rate nearly to zero. At the accumulated dose of CSL up to 80 micrograms.kg-1 (i.v.), the inhibition of Apo was attenuated and the firing activity was restored to predrug level.
  
  
  CONCLUSIONS
  CSL showed an antagonistic action, an action to D2 autoreceptors.
authors.affiliation: Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Chen
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Guo
mesh_terms: D000818:Animals; D017660:Autoreceptors / Q000187:drug effects*; D001600:Berberine Alkaloids / Q000494:pharmacology*; D003342:Corpus Striatum / Q000378:metabolism; D018492:Dopamine Antagonists / Q000494:pharmacology*; D007980:Levodopa / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000187:drug effects*; D013378:Substantia Nigra / Q000502:physiology
chemical_list: C099468:12-chloroscoulerine; D017660:Autoreceptors; D001600:Berberine Alkaloids; D018492:Dopamine Antagonists; D017448:Receptors, Dopamine D2; D007980:Levodopa