title: Amphetamine-induced behavior, dopamine release, and c-fos mRNA expression: modulation by environmental novelty.
abstract: We have shown recently that the psychomotor activating effects of amphetamine in the rat are much greater when this drug is administered in association with environmental novelty than when it is given in a home environment. The main purpose of the present study was to explore the neural basis of this phenomenon. We found, using in situ hybridization of c-fos mRNA, that the pattern of neuronal activation in the cortex, in the caudate, in the shell and core of the nucleus accumbens, and in other subcortical structures was markedly different when amphetamine (2.0 mg/kg, i.p.) was given in association with exposure to environmental novelty relative to when it was given at home. In most brain regions the magnitude of c-fos expression was over two times greater in rats given amphetamine plus novelty than in rats given amphetamine alone. In contrast, an in vivo microdialysis study indicated that environmental novelty did not affect amphetamine-induced dopamine release in either caudate or nucleus accumbens. Furthermore, a unilateral 6-hydroxydopamine lesion of the mesostriatal dopamine system reduced amphetamine- but not novelty-induced c-fos expression. Finally, we found no differences in the amount of corticosterone secreted after exposure to novelty, amphetamine, or both, suggesting that corticosterone does not play a critical role in the ability of novelty to modulate amphetamine-induced psychomotor activation. In conclusion, it seems that environmental novelty alters the neurobiological effects of amphetamine independently of the primary neuropharmacological actions of this drug in the striatum.
authors.affiliation: Biopsychology, Department of Psychology, The University of Michigan, Ann Arbor, Michigan 48109-1109, USA.
authors.forename: A
authors.initials: A
authors.lastname: Badiani
authors.forename: M M
authors.initials: MM
authors.lastname: Oates
authors.forename: H E
authors.initials: HE
authors.lastname: Day
authors.forename: S J
authors.initials: SJ
authors.lastname: Watson
authors.forename: H
authors.initials: H
authors.lastname: Akil
authors.forename: T E
authors.initials: TE
authors.lastname: Robinson
mesh_terms: D000661:Amphetamine / Q000494:pharmacology*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects* / Q000502:physiology; D002421:Caudate Nucleus / Q000187:drug effects / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000502:physiology; D003345:Corticosterone / Q000097:blood; D004298:Dopamine / Q000378:metabolism*; D004777:Environment; D015870:Gene Expression / Q000187:drug effects; D017403:In Situ Hybridization; D008297:Male; D017551:Microdialysis; D009714:Nucleus Accumbens / Q000187:drug effects / Q000378:metabolism; D016627:Oxidopamine / Q000494:pharmacology; D016760:Proto-Oncogene Proteins c-fos / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D016627:Oxidopamine; D000661:Amphetamine; D004298:Dopamine; D003345:Corticosterone