Comparison of two PCRs for detection of Mycobacterium ulcerans.
Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.
Guimaraes-Peres
A
A
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium.
Portaels
F
F
de Rijk
P
P
Fissette
K
K
Pattyn
S R
SR
van Vooren
J
J
Fonteyne
P
P
D001431:Bacteriological Techniques; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D006801:Humans; D019911:Mycobacterium ulcerans / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S