Secretion of ovarian inhibin and its physiologic roles in the regulation of follicle-stimulating hormone secretion during the estrous cycle of the female guinea pig.
To characterize inhibin secretion during the estrous cycle in guinea pigs, the concentrations of plasma inhibin, estradiol, progesterone, and FSH were determined. A significant positive correlation was observed between inhibin and estradiol throughout the estrous cycle. Plasma inhibin and estradiol started to increase a few days before ovulation (Day 0 = day of estimated ovulation), and decreased after ovulation. These two hormones remained low during the luteal phase. The immunoreactivity of inhibin alpha, betaA, and betaB subunits was colocalized in the granulosa cells of one or two healthy large follicles in the ovary before ovulation. There was no positive reaction of inhibin alpha and beta subunits in the corpora lutea or other follicles. Ovariectomy resulted in an abrupt decrease in plasma inhibin and a significant increase in plasma FSH. Injection of anti-inhibin serum into adult female guinea pigs induced an elevation in plasma FSH in a dose-dependent manner. This report presents the first description of sequential changes in plasma inhibin and estradiol during the estrous cycle of guinea pigs. Results suggest that inhibin is secreted mainly by granulosa cells of a few healthy large follicles in the ovary and that it plays an important role in the regulation of FSH secretion during the estrous cycle in guinea pigs.
Shi
F
F
Laboratory of Veterinary Physiology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
Ozawa
M
M
Komura
H
H
Yang
P
P
Trewin
A L
AL
Hutz
R J
RJ
Watanabe
G
G
Taya
K
K
D000818:Animals; D003338:Corpus Luteum / Q000737:chemistry; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000502:physiology*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000378:metabolism*; D006107:Granulosa Cells / Q000737:chemistry; D006168:Guinea Pigs; D006706:Homeostasis; D007116:Immunization, Passive; D007150:Immunohistochemistry; D007265:Inhibins / Q000097:blood / Q000378:metabolism* / Q000502:physiology*; D006080:Ovarian Follicle / Q000737:chemistry; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism*; D010060:Ovulation / Q000502:physiology; D011374:Progesterone / Q000097:blood; D011863:Radioimmunoassay
D011374:Progesterone; D004958:Estradiol; D007265:Inhibins; D005640:Follicle Stimulating Hormone