title: Optimal activation of isopsoralen to prevent amplicon carryover.
abstract: We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5 degrees C. Our data suggest that use of the HRI-300 reaction chamber at 5 degrees C results in a statistically significantly higher degree of amplicon neutralization.
authors.affiliation: Microbiology Service, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. gfahle@nih.gov
authors.forename: G A
authors.initials: GA
authors.lastname: Fahle
authors.forename: V J
authors.initials: VJ
authors.lastname: Gill
authors.forename: S H
authors.initials: SH
authors.lastname: Fischer
mesh_terms: D017931:DNA Primers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry*; D010777:Photochemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D014466:Ultraviolet Rays
chemical_list: D017931:DNA Primers; D005456:Fluorescent Dyes; D011564:Furocoumarins; C011659:angelicin