title: Local transposition of a hobo element within the decapentaplegic locus of Drosophila.
abstract: We have efficiently mobilized a phenotypically silent hobo transgene inserted within the cis-regulatory heldout region of the decapentaplegic (dpp) locus in Drosophila melanogaster. The goal of our experiment was to identify germline transmission of a local transposition event within the dpp locus that meets two specific criteria. First, excision of the hobo construct does not generate an adult mutant phenotype, suggesting minimal alteration to the original site of insertion. Second, we required a new insertion of the hobo transgene into the Haploinsufficient region of the locus approximately 25 kb away. Genetic and molecular criteria are used to evaluate candidate germlines. In a pilot study, this local transposition event occurred independently in two individuals. Both of the transposition events appear to be new insertions into the dpp transcription unit. One insertion is between the two protein-coding exons, and the other is in the 3'-untranslated region of exon three. Strains carrying these insertions are valuable new reagents for the analysis of dpp function and molecular evolution. These results further support the use of the hobo system as an important tool in Drosophila genetics.
authors.affiliation: Department of Biology, Arizona State University, Tempe, Arizona 85287-1501, USA. newfeld@asu.edu
authors.forename: S J
authors.initials: SJ
authors.lastname: Newfeld
authors.forename: N T
authors.initials: NT
authors.lastname: Takaesu
mesh_terms: D000818:Animals; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D017344:Genes, Insect; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D019895:Transposases
chemical_list: D029721:Drosophila Proteins; D019476:Insect Proteins; C064196:dpp protein, Drosophila; D019895:Transposases