Characterization of Plasmodium falciparum glutamate dehydrogenase-soluble antigen.
The major aim of this study was to characterize a soluble Plasmodium falciparum antigen from the plasma of malaria-infected humans and Plasmodium falciparum culture supernatants, using immunoabsorbent techniques and Western blotting. An Mr 60-kDa protein was isolated from the plasma of patients with Plasmodium falciparum malaria by affinity chromatography using rabbit anti-Proteus spp GDH(NADP+) serum as ligand. This protein, present in plasma of patients with acute Plasmodium falciparum infection, in Plasmodium falciparum culture supernatants, and in immune complexes, was tested with Plasmodium falciparum malaria hyperimmune serum from patients living in hyperendemic areas and rabbit anti-Proteus spp GDH(NADP+) serum prepared in the laboratory. In this report, we describe the results of a study showing that parasite GDH(NADP+) can be used to detect the presence of Plasmodium falciparum. It appears that this technique permits the chromatographic detection of a Plasmodium falciparum excretion antigen that may be used in the production of monoclonal antibodies to improve immunodiagnostic assays for the detection of antigenemia, and opens the possibility of its use as a non-microscopic screening method.
Rodríguez-Acosta
A
A
Seccion de Inmunoquimica, Instituto de Medicina Tropical, Universidad Central de Venezuela, Caracas, Venezuela. rodriguf@camelot.rect.ucv.ve
Domínguez
N G
NG
Aguilar
I
I
Girón
M E
ME
D000208:Acute Disease; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan / Q000097:blood*; D003470:Culture Media; D005969:Glutamate Dehydrogenase / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D016778:Malaria, Falciparum / Q000097:blood / Q000201:enzymology* / Q000469:parasitology; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000302:isolation & purification; D011817:Rabbits; D012995:Solubility
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D000953:Antigens, Protozoan; D003470:Culture Media; D007074:Immunoglobulin G; D005969:Glutamate Dehydrogenase