title: [Studies on selectivity of recognizing factor for rmf promoter].
abstract: The truncted DNA template carrying rmf gene promoter was transcripted by E. coli RNA polymorase holoenzyme (E sigma) reconstituted with core enzyme (alpha 2 beta beta') and sigma 70 or sigma 38 in vitro. The initional site of the transcription of rmf was confirmed with different restriction endonuclease. rmf promoter can be recognized by E sigma 70 but not E sigma 38. The suitable temperature for in vitro transcription was 37 degrees C, NaCl concentration was 50 mmol/L.
authors.affiliation: Wuhan Institute of Virology, Academia Sinica.
authors.forename: Q
authors.initials: Q
authors.lastname: Ding
authors.forename: I
authors.initials: I
authors.lastname: Akira
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005796:Genes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
chemical_list: D029968:Escherichia coli Proteins; D012269:Ribosomal Proteins; C063545:ribosome modulation factor, E coli; D012321:DNA-Directed RNA Polymerases