Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells.
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 micrograms/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Ishimaru
D
D
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Marcicano
F G
FG
Rebello
M A
MA
D000524:Alphavirus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D018354:Alphavirus Infections / Q000188:drug therapy; D000818:Animals; D002522:Chlorocebus aethiops; D006023:Glycoproteins / Q000096:biosynthesis; D008715:Methionine / Q000032:analysis; D011454:Prostaglandins A / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D011456:Prostaglandins B / Q000378:metabolism / Q000494:pharmacology* / Q000627:therapeutic use; D011486:Protein C / Q000096:biosynthesis; D014709:Vero Cells / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects*
D006023:Glycoproteins; D011454:Prostaglandins A; D011456:Prostaglandins B; D011486:Protein C; D008715:Methionine