Recognition of a non-standard base pair by thermostable DNA polymerases.
Examination of several commercially available thermostable DNA polymerases identifies 9 degrees N DNA polymerase as single enzyme that could incorporate two components of an expanded genetic alphabet, 2,4-diaminopyrimidine and xanthosine as deoxynucleoside triphosphate opposite their cognate base in a DNA template.
Lutz
M J
MJ
Department of Chemistry, ETH Zürich, Switzerland. michael.lutz@pharma.novartis.com
Horlacher
J
J
Benner
S A
SA
D020029:Base Pairing; D001483:Base Sequence; D017931:DNA Primers; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D004795:Enzyme Stability; D006860:Hydrogen Bonding; D011743:Pyrimidines; D019711:Pyrococcus / Q000201:enzymology; D012263:Ribonucleosides; D013379:Substrate Specificity; D013698:Templates, Genetic; D013816:Thermodynamics; D013824:Thermus / Q000201:enzymology; D014970:Xanthines
D017931:DNA Primers; D003854:Deoxyribonucleotides; D011743:Pyrimidines; D012263:Ribonucleosides; D014970:Xanthines; C017205:2,4-diaminopyrimidine; C005893:xanthosine; D004259:DNA-Directed DNA Polymerase