title: Virulence properties of Escherichia coli 83972, a prototype strain associated with asymptomatic bacteriuria.
abstract: Little is known about bacteria associated with asymptomatic bacteriuria (ABU) with regard to urinary tract colonization mechanisms. In this study, virulence properties of Escherichia coli 83972, a strain that was isolated from a clinical ABU episode, were examined. The genetic potential for expression of P and type 1 pili was demonstrated, and DNA sequences related to type 1C and G (UCA) pilus genes were also detected. However, E. coli 83972 did not express D-mannose-resistant or D-mannose-sensitive hemagglutination after growth under standard conditions in vitro or upon isolation from the urine of colonized test subjects. Limited uroepithelial cell adherence was observed in vivo, and weak D-mannose-sensitive hemagglutination was detected after extended growth in urine in vitro.
authors.affiliation: Department of Microbiology and Immunology, Baylor College of Medicine, University of Texas Health Science Center, Houston, Texas, USA. rhull@bcm.tmc.edu
authors.forename: R A
authors.initials: RA
authors.lastname: Hull
authors.forename: D C
authors.initials: DC
authors.lastname: Rudy
authors.forename: W H
authors.initials: WH
authors.lastname: Donovan
authors.forename: I E
authors.initials: IE
authors.lastname: Wieser
authors.forename: C
authors.initials: C
authors.lastname: Stewart
authors.forename: R O
authors.initials: RO
authors.lastname: Darouiche
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics; D000483:Alleles; D001437:Bacteriuria / Q000382:microbiology*; D001483:Base Sequence; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology*; D005260:Female; D036781:Fimbriae Proteins; D010861:Fimbriae, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D010641:Phenotype; D017422:Sequence Analysis, DNA; D001750:Urinary Bladder, Neurogenic / Q000382:microbiology; D014774:Virulence
chemical_list: D018830:Adhesins, Escherichia coli; C092426:PapG protein, E coli; D036781:Fimbriae Proteins