Responses to human rhinovirus in CD45 T cell subsets isolated from tonsil.
Isotypes of CD45 have been used extensively as markers of memory and naive populations of T cells in peripheral blood. In this study, T cells were isolated from human tonsil and their proliferative response against human rhinovirus was measured. Unexpectedly, equivalent responses were found among the CD4+CD45RA+ and CD4+CD45RO+ populations of T cells. This response requires MHC class II-positive antigen-presenting cells. The time course of the T cell response in vitro was that of a classical recall response, and no proliferative response to the virus could be detected in human cord blood. These results suggest that tonsils contain a significant population of CD45RA+ memory cells. The presence of this population may reflect ongoing stimulation with this common infectious agent, and the anatomical location of the T cells within the major lymphoid organ draining the naso-pharyngeal epithelial surface.
Wilamasundera
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Department of Immunology, Windeyer Institute of Medical Sciences, University College London, GB.
Katz
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D017951:Antigen Presentation; D000956:Antigens, Viral / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D017493:Leukocyte Common Antigens / Q000276:immunology*; D014066:Palatine Tonsil / Q000166:cytology / Q000276:immunology*; D012229:Rhinovirus / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology
D000956:Antigens, Viral; D017493:Leukocyte Common Antigens