Transcranial laser doppler mapping of activation flow coupling of the rat somatosensory cortex.
Signal averaged laser Doppler (LD) through a thinned skull over the rat somatosensory cortex was used to map the spatial and temporal characteristics of activation-flow coupling, the change in regional cerebral blood flow (CBF) due to neuronal activation, in response to electrical forepaw stimulation. The location of maximal changes in amplitude of the LD response was reproducibly recorded at 4-5 mm lateral to Bregma. This location is very similar but slightly posterior and lateral to results obtained from electrophysiological, magnetic resonance imaging (MRI), and optical imaging studies. The latency of the activation-flow coupling (AFC) response was inversely correlated to response amplitude, with shorter latencies at positions of maximal amplitude.
Ances
B M
BM
Department of Neurology, University of Pennsylvania, Philadelphia 19104, USA.
Detre
J A
JA
Takahashi
K
K
Greenberg
J H
JH
D000818:Animals; D001931:Brain Mapping; D002560:Cerebrovascular Circulation; D004558:Electric Stimulation; D005552:Forelimb / Q000294:innervation; D017078:Laser-Doppler Flowmetry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012815:Signal Processing, Computer-Assisted; D013003:Somatosensory Cortex / Q000098:blood supply* / Q000502:physiology*; D013997:Time Factors