Diversity among clinical isolates of Proteus penneri detected by random amplified polymorphic DNA analysis.
DNA of thirteen haemolytic Proteus penneri strains of clinical origin, all producing calcium dependent haemolysin and having been derived from four European countries was examined for plasmid profile, and outer membrane protein profile, by random amplified polymorphic DNA-PCR (RAPD-PCR) method, and digestions with restriction endonucleases were performed. All strains contained two large plasmids of approximately 60 and 70 kilobase pairs (kb). In addition, four strains contained a small plasmid of about 6 kb. These four strains produced cell-bound haemolysin only. Outer membrane protein analysis revealed subtle differences between strains. RAPD-PCR with primer I (CCGCAGCCAA) revealed 13 types, whereas primer II (AACGCGCAAC) yielded only two main types of different patterns. Results with primer I suggests a DNA sequence diversity within this species. The RAPD-PCR method provides a fast, economical and reproducible means for the typing of P. penneri. Digestion with restriction endonucleases indicated a high level of DNA methylation in this species.
Hoffmann
G
G
Department of Genetics and Microbiology, Janus Pannonius University, Pécs, Hungary.
Gajdos
G
G
Czakó
M
M
Kerényi
M
M
Tóth
V
V
Emõdy
L
L
Tomcsányi
T
T
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis; D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D014644:Genetic Variation; D006801:Humans; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011511:Proteus / Q000737:chemistry / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D011512:Proteus Infections / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique; D015183:Restriction Mapping
D001425:Bacterial Outer Membrane Proteins; D004269:DNA, Bacterial