Rapid staining of ultrathin sections with the use of a microwave oven.
The microwave oven has many potential applications, ranging from tissue fixation to staining for light and electron microscopy. This study was planned to speed up the staining of ultrathin sections. The first set of grids was stained conventionally with uranyl acetate and Reynold's lead citrate solutions. The other grids were stained with the same solutions by microwave irradiation. The electron micrographs of grids stained using the microwave technique were as satisfactory as the grids stained conventionally. Microwave-treated grids demonstrated more uniform staining and less precipitate. The use of a microwave oven shortened staining time by approximately 38 min.
Cavusoglu
I
I
Uludag University Faculty of Medicine, Department of Histology & Embryology, Bursa, Turkey. Ilkin@uu20.bim.uludag.edu.tr
Kahveci
Z
Z
Sirmali
S A
SA
D000818:Animals; D007668:Kidney / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D008867:Microtomy; D008872:Microwaves; D009942:Organometallic Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling
D009942:Organometallic Compounds; C005460:uranyl acetate