A study of metabolites isolated from the urine samples of cats and dogs administered orbifloxacin.
Urine samples of cats and dogs collected for 24 hr after a subcutaneous injection of orbifloxacin (OBFX) were analyzed. The metabolites were examined using HPLC. In the dog urine, 87% of total was the parent compound and 13% glucuronide compound of OBFX and 96% was parent and 4% metabolite in the cat urine. The metabolite of cat urine was identified as N-hydroxy OBFX, determined by comparison of the extraction of urine with chloroform with the standard compound of N-hydroxy OBFX, using LC/APCIMS. N-hydroxy OBFX had a weaker antibacterial activity against fluoroquinolone sensitive bacteria than the parent compound.
Matsumoto
S
S
Research Laboratories, Animal Science Division, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Takahashi
M
M
Kitadai
N
N
Katae
H
H
D000818:Animals; D000900:Anti-Bacterial Agents / Q000493:pharmacokinetics* / Q000652:urine; D002415:Cats / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000652:urine; D004285:Dogs / Q000652:urine*; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests / Q000662:veterinary
D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; C095163:orbifloxacin