Comparison of hemoglobin A1C results by two different methods on patients with structural hemoglobin variants.
OBJECTIVE
The objective was to compare hemoglobin A1C (HbA1C) results obtained by two methods based on different analytical principles for individuals with a structural hemoglobin variant.


METHODS
Hemoglobin A1C results were obtained using the Bio-Rad Variant (based on cation exchange chromatography) and the Bayer DCA 2000 (based on an immunological reaction) on individuals with a structural hemoglobin variant. The identity of the hemoglobin variant was confirmed by high pressure liquid chromatography (HPLC) and electrophoresis.


RESULTS
Hemoglobin A1C results obtained by the two methods on individuals with S, C, D, and E trait were in close agreement.


CONCLUSIONS
The Bio-Rad Variant and Bayer DCA 2000 produce equivalent hemoglobin A1C results on patients with S, C, and E trait. With appropriate correction, correlation of hemoglobin A1C results from the Bio-Rad Variant for individuals with D trait was good (r = 0.927). Glycohemoglobin results obtained by the two methods for some unusual structural hemoglobin variants were in close agreement.
Blakney
G B
GB
Chemistry Department, Dynacare Kasper Medical Laboratories, Edmonton, Alberta, Canada.
Higgins
T N
TN
Holmes
D J
DJ
D002852:Chromatography, Ion Exchange / Q000379:methods*; D006442:Glycated Hemoglobin / Q000032:analysis*; D006444:Hemoglobin C / Q000032:analysis; D006446:Hemoglobin E / Q000032:analysis; D006451:Hemoglobin, Sickle / Q000032:analysis; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*
D006442:Glycated Hemoglobin; D006444:Hemoglobin C; D006446:Hemoglobin E; D006451:Hemoglobin, Sickle; D006455:Hemoglobins, Abnormal; C032001:hemoglobin D