Protective ability of acetylsalicylic acid (aspirin) to scavenge radiation induced free radicals in J774A.1 macrophage cells.
Radiation generates a variety of free radicals during the exposure of biological tissues through radiolysis of water. These free radicals are highly reactive and cause oxidative damage to biological molecules. This study examined the protective ability of aspirin against radiation induced oxidative stress. The study assessed the protective effect of aspirin (0.05 mM, 0.10 mM, 0.50 mM) on the generation of free radicals during exposure of J774A.1 macrophage cells to radiation (13.25 cGy). Approximately a 2.2-fold increase in superoxide anion formation as determined by cytochrome c reduction was observed following exposure of the cells to radiation for 20 one second exposures. Preincubation with aspirin exhibited a dose dependent decrease in free radical production as assessed by chemiluminescence and cytochrome c reduction. Aspirin also produced a concentration dependent reduction in radiation induced DNA damage in the cells. The data indicate that radiation of these cells results in production of reactive oxygen species and DNA damage, and aspirin can decrease these effects in a concentration dependent manner.
Saini
T
T
School of Dentistry, Creighton University, Omaha, Nebraska, USA.
Bagchi
M
M
Bagchi
D
D
Jaeger
S
S
Hosoyama
S
S
Stohs
S J
SJ
D000704:Analysis of Variance; D000818:Animals; D001241:Aspirin / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D003574:Cytochrome c Group / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers; D005609:Free Radicals / Q000378:metabolism; D008163:Luminescent Measurements; D008264:Macrophages / Q000187:drug effects* / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects; D011833:Radiation Injuries, Experimental / Q000378:metabolism / Q000517:prevention & control; D017382:Reactive Oxygen Species / Q000378:metabolism*
D016861:Cyclooxygenase Inhibitors; D003574:Cytochrome c Group; D016166:Free Radical Scavengers; D005609:Free Radicals; D017382:Reactive Oxygen Species; D001241:Aspirin