title: Genotyping for polymorphisms in interferon-gamma, interleukin-10, transforming growth factor-beta 1 and tumour necrosis factor-alpha genes: a technical report.
abstract: Polymorphic variants of cytokine genes are associated with acute and chronic transplant rejection. In this technical report, the methods currently used in our centre to genotype individuals for interferon-gamma, interleukin-10, transforming growth factor-beta 1 and tumour necrosis factor-alpha are described in detail. The DNA sequences of primers and probes, and conditions for polymerase chain reactions are given, and the allele and genotype frequencies in our control populations are summarized.
authors.affiliation: School of Biological Sciences, University of Manchester, UK.
authors.forename: C
authors.initials: C
authors.lastname: Perrey
authors.forename: V
authors.initials: V
authors.lastname: Pravica
authors.forename: P J
authors.initials: PJ
authors.lastname: Sinnott
authors.forename: I V
authors.initials: IV
authors.lastname: Hutchinson
mesh_terms: D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D011110:Polymorphism, Genetic; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*
chemical_list: D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma