title: First synthesis of alternating SATE-phosphotriester/phosphodiester prooligonucleotides on solid support.
abstract: Prooligonucleotides with alternating S-Acyl-ThioEthyl (SATE) phosphotriester and phosphodiester linkages or a gap were synthesized by elimination of cyanoethyl group with non-nucleophilic base DBU. Their half-lives in the presence of Pig Liver Esterases (PLE) and in total cell extract were determined.
authors.affiliation: Laboratoire de Chimie Bio-Organique, UMR 5625, Université de Montpellier II, France.
authors.forename: G
authors.initials: G
authors.lastname: Tosquellas
authors.forename: J C
authors.initials: JC
authors.lastname: Bologna
authors.forename: F
authors.initials: F
authors.lastname: Morvan
authors.forename: B
authors.initials: B
authors.lastname: Rayner
authors.forename: J L
authors.initials: JL
authors.lastname: Imbach
mesh_terms: D000818:Animals; D002457:Cell Extracts; D004950:Esterases / Q000378:metabolism; D006207:Half-Life; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013552:Swine; D013936:Thymidine / Q000737:chemistry
chemical_list: D002457:Cell Extracts; D009841:Oligonucleotides; D004950:Esterases; D013936:Thymidine