title: Restoration of the p16 gene is related to increased radiosensitivity of p16-deficient lung adenocarcinoma cell lines.
abstract: To assess the role played by p16 gene expression in the radiosensitivity of human lung cancers, we transferred exogenous p16 genes into p16-deficient H460 and A549 lung adenocarcinoma cell lines and compared the cell survival curve in vitro after irradiation. The surviving fraction of the p16-transfected A549p16 and H460p16 cells that expressed exogenous p16 mRNA or protein was lower than those of the parental and negative control cells. The rapid exit of the p16-transfected cells from the G2/M phase in the cell cycle, both before and after irradiation, possibly contributes to the increased radiosensitivity of our experimental p16-transfected lung adenocarcinoma cell lines. We conclude that exogenous p16 gene may be another important factor controlling the intrinsic cellular radiosensitivity of cancer.
authors.affiliation: Department of Radiotherapy, Cancer Hospital, Shanghai Medical University, PR China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Fu
authors.forename: S W
authors.initials: SW
authors.lastname: Zhang
authors.forename: R Q
authors.initials: RQ
authors.lastname: Ran
authors.forename: Z H
authors.initials: ZH
authors.lastname: Shen
authors.forename: J X
authors.initials: JX
authors.lastname: Gu
authors.forename: S L
authors.initials: SL
authors.lastname: Cao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000532:radiotherapy; D002453:Cell Cycle; D002470:Cell Survival / Q000528:radiation effects; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D018014:Gene Transfer Techniques; D019942:Genes, p16; D008175:Lung Neoplasms / Q000235:genetics* / Q000532:radiotherapy; D011836:Radiation Tolerance; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16; D011994:Recombinant Proteins