title: The survival of adenovirus in multidose bottles of topical fluorescein.
abstract: OBJECTIVE
  To determine if common ocular adenovirus serotypes survive in vitro in multidose bottles of topical fluorescein (Fluress; Pilkington Barnes Hind, Inc, Sunnyvale, California).
  
  
  METHODS
  Clinical isolates of adenovirus types 8 and 19 were inoculated separately into 10 bottles each of Fluress and maintained at room temperature (25 C). All bottles were titered for adenovirus on A549 cell monolayers at 0, 7, 14, 21, 28, and 49 days.
  
  
  RESULTS
  Adenovirus was recovered from Fluress for up to 21 days for adenovirus type 19 and 28 days for adenovirus type 8.
  
  
  CONCLUSIONS
  A multidose bottle of Fluress contaminated with adenovirus can be a potential source of adenoviral transmission in an ophthalmic office setting.
authors.affiliation: Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania, USA. Rege@vision.eei.upmc.edu
authors.forename: R P
authors.initials: RP
authors.lastname: Kowalski
authors.forename: E G
authors.initials: EG
authors.lastname: Romanowski
authors.forename: B
authors.initials: B
authors.lastname: Waikhom
authors.forename: Y J
authors.initials: YJ
authors.lastname: Gordon
mesh_terms: D000258:Adenovirus Infections, Human / Q000635:transmission / Q000821:virology; D000260:Adenoviruses, Human / Q000502:physiology*; D000287:Administration, Topical; D002724:Chlorobutanol; D004338:Drug Combinations; D004340:Drug Contamination; D004492:Edetic Acid; D015828:Eye Infections, Viral / Q000635:transmission / Q000821:virology; D005452:Fluoresceins; D006801:Humans; D009883:Ophthalmic Solutions; D011205:Povidone; D011343:Procaine / Q000031:analogs & derivatives*; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D004338:Drug Combinations; D005452:Fluoresceins; D009883:Ophthalmic Solutions; C010961:Fluress; D011343:Procaine; D004492:Edetic Acid; D011205:Povidone; D002724:Chlorobutanol