Expression of the nicotinic acetylcholine receptor alpha4 subunit mRNA in the rat cerebellar cortex.
In order to compare the previous immunohistochemical and immunocytochemical data on the distribution of nicotinic acetylcholine receptor alpha4 subunit-like immunoreactivity with the expression of alpha4 mRNA in the rat cerebellar cortex, the present study determined the cellular distribution of alpha4 mRNA in the rat cerebellar cortex. Northern blot analysis revealed two alpha4 mRNA bands in the rat cerebellum and three in the cerebral cortex, hippocampus, hypothalamus and striatum. The total level of these transcripts was lower in the cerebellum than in the other four regions. The expression of alpha4 mRNA was high in Purkinje cells and granular cells, whereas low expression was detected in the molecular layer. These results suggest that the expression of alpha4 mRNA is closely related to the alpha4-like immunoreactivity in the molecular and Purkinje cell layers. In the granular layer, alpha4 mRNA was very highly and broadly expressed in comparison with the alpha4-like immunoreactivity.
Nakayama
H
H
Department of Pharmacology, Nara Medical University, Kashihara, Japan.
Shioda
S
S
Nakajo
S
S
Ueno
S
S
Nakai
Y
Y
D000818:Animals; D001345:Autoradiography; D002525:Cerebellar Cortex / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D015870:Gene Expression; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis*; D051381:Rats; D011978:Receptors, Nicotinic / Q000235:genetics*
D012333:RNA, Messenger; D011978:Receptors, Nicotinic