title: Molecular cytogenetic mapping of 24 CEPH YACs and 24 gene-specific large insert probes to chromosome 17.
abstract: Defining boundaries of chromosomal rearrangements at the molecular level would benefit from landmarks that link the cytogenetic map to physical, genetic, and transcript maps, as well as from large-insert FISH probes for such loci to detect numerical and structural rearrangements in metaphase or interphase cells. Here, we determined the locations of 24 genetically mapped CEPH-Mega YACs along the FLpter scale (fractional length from p-telomere) by quantitative fluorescence in situ hybridization analysis. This generated a set of cytogenetically mapped probes for chromosome 17 with an average spacing of about 5 cM. We then developed large-insert YAC, BAC, PAC, or P1 clones to the following 24 known genes, and determined refined map locations along the same FLpter scale: pter-TP53-TOP3-cen-TNFAIP1-ERBB2-TOP2A- BRCA1-TCF11-NME1-HLF-ZNF147/CL N80-BCL5/MPO/SFRS1-TBX2-PECAM1-DDX5/ PRKCA-ICAM2-GH1/PRKAR1A-GRB2-CDK3 /FKHL13-qter. Taken together, these 48 cytogenetically mapped large-insert probes provide tools for the molecular analysis of chromosome 17 rearrangements, such as mapping amplification, deletion, and translocation breakpoints in this chromosome, in cancer and other diseases.
authors.affiliation: Laboratory of Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, Tampere (Finland).
authors.forename: M
authors.initials: M
authors.lastname: Bärlund
authors.forename: N N
authors.initials: NN
authors.lastname: Nupponen
authors.forename: R
authors.initials: R
authors.lastname: Karhu
authors.forename: M M
authors.initials: MM
authors.lastname: Tanner
authors.forename: P
authors.initials: P
authors.lastname: Paavola
authors.forename: O P
authors.initials: OP
authors.lastname: Kallioniemi
authors.forename: A
authors.initials: A
authors.lastname: Kallioniemi
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D018244:Chromosomes, Artificial, Yeast; D002886:Chromosomes, Human, Pair 17; D015342:DNA Probes; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D015003:Yeasts / Q000235:genetics*
chemical_list: D015342:DNA Probes