Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative.
A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and detected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N = 3).
Kim
K H
KH
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Lee
J G
JG
Park
J H
JH
Shin
Y G
YG
Lee
S K
SK
Cho
T H
TH
Oh
S T
ST
D000873:Anthracenes / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis / Q000097:blood*; D005456:Fluorescent Dyes; D005960:Glucosides / Q000032:analysis / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
D000873:Anthracenes; D002867:Chromones; D005456:Fluorescent Dyes; D005960:Glucosides; C066291:9-anthroylnitrile; C069868:aloesin