Structure of sulfated O-specific polysaccharide of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232.
A sulfated O-specific polysaccharide containing D-mannose, L-rhamnose, and the sulfate group was obtained by mild acid hydrolysis of lipopolysaccharide (S-form) of the marine bacterium Pseudoalteromonas marinoglutinosa KMM 232. Based on analysis of methylation and 13C-NMR spectroscopy of native and desulfated polysaccharides, the following structure of disaccharide repeat unit in the O-specific polysaccharide has been established: [scheme]. This is the first report of a sulfated O-specific polysaccharide isolated from gram-negative bacteria.
Komandrova
N A
NA
Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, Vladivostok, 690022, Russia. elnaz@piboc.marine.su
Tomshich
S V
SV
Isakov
V V
VV
Romanenko
L A
LA
D002240:Carbohydrate Sequence; D006088:Gram-Negative Aerobic Bacteria / Q000737:chemistry* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D019081:O Antigens / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012623:Seawater / Q000382:microbiology; D013431:Sulfates / Q000737:chemistry
D019081:O Antigens; D011135:Polysaccharides, Bacterial; D013431:Sulfates