Glyoxylate cycle enzymes are present in liver peroxisomes of alloxan-treated rats.
Key enzymes of the glyoxylate cycle, isocitrate lyase (ICL) and malate synthase (MS), have been detected in the liver of alloxan-treated rats. The activity of ICL in rat liver was 0.040 micromol/min/mg protein and the activity of MS was 0.022 micromol/min/mg protein. These enzymes were associated with the peroxisomal fraction. The activities of citrate synthase, malate synthase and malate dehydrogenase detected in the peroxisomal fraction were also increased by alloxan treatment. Isocitrate lyase was partially purified and displayed catalytic and regulatory properties similar to those of the enzyme isolated from the liver of starved rats (Popov, V.N. et al. (1996) FEBS Lett. 391, 87-90).
Popov
V N
VN
Department of Plant Physiology and Biochemistry, Voronezh State University, Russia. vasily@bc.vsu.ru
Volvenkin
S V
SV
Eprintsev
A T
AT
Igamberdiev
A U
AU
D000154:Aconitate Hydratase / Q000378:metabolism; D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D002458:Cell Fractionation; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007522:Isocitrate Lyase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007668:Kidney; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008291:Malate Dehydrogenase / Q000378:metabolism; D008292:Malate Synthase / Q000378:metabolism*; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009928:Organ Specificity; D051381:Rats
D006038:Glyoxylates; D000496:Alloxan; D008291:Malate Dehydrogenase; D002950:Citrate (si)-Synthase; D008292:Malate Synthase; D007522:Isocitrate Lyase; D000154:Aconitate Hydratase; C031150:glyoxylic acid