Comparison of nuclear matrix proteins between EGFR-antisense transfected and untransfected glioblastoma cells.
The protein composition of the nuclear matrix is both tissue and cell type specific, and it undergoes changes with differentiation and transformation. In the present study, nuclear matrix proteins of EGFR-antisense transfected glioblastoma cell lines, U87 and U343, were compared with untransfected cell lines using two dimensional-gel electrophoresis. After EGFR-antisense transfection, the protein compositions of the nuclear matrices in both cell lines were different. Several nuclear proteins were only found in EGFR-antisense transfected cell lines. There was no difference in NuMA expression in the transfected and untransfected cell lines. These results suggest that EGFR-antisense reduced tumorigenicity on human glioblastoma cells by changing nuclear matrix protein compositions.
Wang
ZH
Department of Anatomy, The Chinese University of Hong Kong, Shatin, N.T. Hong Kong.
Yam
GHF
Tian
XX
Ng
HK
Ding
MX
Chew-Cheng
SB
Chew
EC