Enhanced differentiation of HL-60 leukemia cells to macrophages induced by ciprofibrate.
Ciprofibrate, an hypolipidaemic peroxisome proliferator, induced differentiation of HL-60 cells. The effect was greatly potentiated by phorbol 12-myristate 13-acetate at a concentration where neither phorbol ester nor ciprofibrate alone had any effect on these cells. As occurs for HL-60 cell differentiation induced by high phorbol ester concentration, the ciprofibrate-induced phorbol ester-dependent differentiation of HL-60 cells proceeded through the monocytic/macrophage pathway and induced the phosphorylation of proteins with similar molecular weights suggesting that increased protein kinase C activity may be involved in the effect. The peroxisome proliferator-activated receptor (PPARalpha) transcription factor is expressed in HL-60 cells, but no changes were observed in its expression upon HL-60 cell differentiation.
Bronfman
M
M
Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, P. Universidad Católica de Chile, Santiago. mbronfma@genes.bio.puc.cl
Ponce
C
C
Rojas
S
S
Roth
A
A
Loyola
G
G
Vollrath
V
V
Chianale
J
J
D018845:CD11 Antigens / Q000096:biosynthesis; D002454:Cell Differentiation / Q000187:drug effects; D002995:Clofibric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004357:Drug Synergism; D058607:Fibric Acids; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology* / Q000378:metabolism; D010766:Phosphorylation; D012333:RNA, Messenger / Q000032:analysis; D018160:Receptors, Cytoplasmic and Nuclear / Q000096:biosynthesis; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors; D014157:Transcription Factors / Q000096:biosynthesis
D018845:CD11 Antigens; D058607:Fibric Acids; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D002995:Clofibric Acid; C019304:ciprofibrate; D013755:Tetradecanoylphorbol Acetate