title: A decrease of reelin expression as a putative vulnerability factor in schizophrenia.
abstract: Postmortem prefrontal cortices (PFC) (Brodmann's areas 10 and 46), temporal cortices (Brodmann's area 22), hippocampi, caudate nuclei, and cerebella of schizophrenia patients and their matched nonpsychiatric subjects were compared for reelin (RELN) mRNA and reelin (RELN) protein content. In all of the brain areas studied, RELN and its mRNA were significantly reduced (approximately 50%) in patients with schizophrenia; this decrease was similar in patients affected by undifferentiated or paranoid schizophrenia. To exclude possible artifacts caused by postmortem mRNA degradation, we measured the mRNAs in the same PFC extracts from gamma-aminobutyric acid (GABA)A receptors alpha1 and alpha5 and nicotinic acetylcholine receptor alpha7 subunits. Whereas the expression of the alpha7 nicotinic acetylcholine receptor subunit was normal, that of the alpha1 and alpha5 receptor subunits of GABAA was increased when schizophrenia was present. RELN mRNA was preferentially expressed in GABAergic interneurons of PFC, temporal cortex, hippocampus, and glutamatergic granule cells of cerebellum. A protein putatively functioning as an intracellular target for the signal-transduction cascade triggered by RELN protein released into the extracellular matrix is termed mouse disabled-1 (DAB1) and is expressed at comparable levels in the neuroplasm of the PFC and hippocampal pyramidal neurons, cerebellar Purkinje neurons of schizophrenia patients, and nonpsychiatric subjects; these three types of neurons do not express RELN protein. In the same samples of temporal cortex, we found a decrease in RELN protein of approximately 50% but no changes in DAB1 protein expression. We also observed a large (up to 70%) decrease of GAD67 but only a small decrease of GAD65 protein content. These findings are interpreted within a neurodevelopmental/vulnerability "two-hit" model for the etiology of schizophrenia.
authors.affiliation: Psychiatric Institute, Department of Psychiatry, College of Medicine, University of Illinois, Chicago, IL 60612, USA.
authors.forename: F
authors.initials: F
authors.lastname: Impagnatiello
authors.forename: A R
authors.initials: AR
authors.lastname: Guidotti
authors.forename: C
authors.initials: C
authors.lastname: Pesold
authors.forename: Y
authors.initials: Y
authors.lastname: Dwivedi
authors.forename: H
authors.initials: H
authors.lastname: Caruncho
authors.forename: M G
authors.initials: MG
authors.lastname: Pisu
authors.forename: D P
authors.initials: DP
authors.lastname: Uzunov
authors.forename: N R
authors.initials: NR
authors.lastname: Smalheiser
authors.forename: J M
authors.initials: JM
authors.lastname: Davis
authors.forename: G N
authors.initials: GN
authors.lastname: Pandey
authors.forename: G D
authors.initials: GD
authors.lastname: Pappas
authors.forename: P
authors.initials: P
authors.lastname: Tueting
authors.forename: R P
authors.initials: RP
authors.lastname: Sharma
authors.forename: E
authors.initials: E
authors.lastname: Costa
mesh_terms: D017668:Age of Onset; D000368:Aged; D017398:Alternative Splicing; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D015816:Cell Adhesion Molecules, Neuronal / Q000096:biosynthesis* / Q000235:genetics*; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics*; D005786:Gene Expression Regulation; D014644:Genetic Variation; D006801:Humans; D051379:Mice; D008818:Mice, Neurologic Mutants; D008875:Middle Aged; D009419:Nerve Tissue Proteins; D009928:Organ Specificity; D000091042:Reelin Protein; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012559:Schizophrenia / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D012697:Serine Endopeptidases; D014158:Transcription, Genetic
chemical_list: D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; C000717127:RELN protein, human; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases