title: The soft tissue cover of the mandibular condyle. Differentiation in histological forms and age-related changes of aggrecan- and versican-like proteoglycans.
abstract: Age-related changes of the composition of the extracellular matrix of the soft tissue cover of the mandibular condyle (STC), especially of the large proteoglycans, have been investigated. Proteoglycans were extracted from the STC of neonatal, juvenile and adult domestic pigs, fractionated by density gradient centrifugation and analyzed by electrophoresis/Western blotting. Experiments revealed firstly that a large CS/KS proteoglycan (aggrecan) is an essential constituent of the STC at all ages. This proteoglycan is required for nutrition of avascular tissues, and age-related changes in its average size and substitution with KS (keratan sulfate) may be a response to altered functional loading and tissue architecture of the STC. Secondly it was shown that a large CS/DS (chondroitin sulfate/dermatan sulfate) proteoglycan characterized by a doublet of core proteins at 200 and 250 kDa, thereby resembling perlecan, is present in the tissue of adults, but not of neonates and juveniles. Thirdly a large CS/DS proteoglycan characterized by core proteins at 350, 450 and 550 kDa, thereby resembling versican, was present in juveniles. It was detectable only weakly in neonates and not in adults. Results of core protein analysis were confirmed by results of agarose gel electrophoresis/Western blotting of the undigested proteoglycans isolated directly from the tissue extracts. Versican is believed to destabilize cell-matrix interactions required for cell proliferation and differentiation. In this context, presence of versican-like proteoglycans in the STC of growing individuals and its disappearance in adults appears to be related to the growth potential of the mandibular condyle.
authors.affiliation: Department of Orthodontics, Leipzig University.
authors.forename: K
authors.initials: K
authors.lastname: Müller
authors.forename: T
authors.initials: T
authors.lastname: Primm
authors.forename: K H
authors.initials: KH
authors.lastname: Dannhauer
mesh_terms: D053674:Aggrecans; D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D015153:Blotting, Western / Q000706:statistics & numerical data; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis / Q000378:metabolism*; D004587:Electrophoresis, Agar Gel / Q000706:statistics & numerical data; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D037181:Lectins, C-Type; D008335:Mandibular Condyle / Q000737:chemistry / Q000378:metabolism*; D011509:Proteoglycans / Q000032:analysis / Q000378:metabolism*; D013552:Swine; D053675:Versicans
chemical_list: D053674:Aggrecans; D011508:Chondroitin Sulfate Proteoglycans; D016326:Extracellular Matrix Proteins; D037181:Lectins, C-Type; D011509:Proteoglycans; D053675:Versicans