title: Increased cyclin E level in retinoblastoma cells during programmed cell death.
abstract: Camptothecin (an inhibitor of topoisomerase I) and etoposide and amsacrine (inhibitors of topoisomerase II) both capable of triggering programmed cell death in Y79 cells, induced a remarkable dose-dependent increase in the level of cyclin E in these cells. Camptothecin was found to be the most effective compound. The effect was not observed when the cells were treated with other inducers of programmed cell death (C2-ceramide, sodium butyrate, interleukin-1beta and tumor necrosis factor), all of which do not damage DNA. The effect, which was completely prevented by inhibitors of macromolecular synthesis, occurred after a lag phase (12 hrs.) and increased concurrently with the rise in programmed cell death (PCD), reaching a maximum after 36 hrs. of incubation, when a large percentage of cells (95%) showed clear PCD signals. We suggest that cyclin E takes part in the final stage of programmed cell death which is induced by topoisomerase inhibitors in Y79 cells.
authors.affiliation: Institute of Biological Chemistry, University of Palermo, Policlinico, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Lauricella
authors.forename: M
authors.initials: M
authors.lastname: Giuliano
authors.forename: S
authors.initials: S
authors.lastname: Emanuele
authors.forename: M
authors.initials: M
authors.lastname: Carabillò
authors.forename: R
authors.initials: R
authors.lastname: Vento
authors.forename: G
authors.initials: G
authors.lastname: Tesoriere
mesh_terms: D000677:Amsacrine / Q000378:metabolism; D017209:Apoptosis / Q000502:physiology*; D002166:Camptothecin / Q000378:metabolism; D019925:Cyclin A / Q000187:drug effects / Q000378:metabolism; D019927:Cyclin E / Q000187:drug effects / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005047:Etoposide / Q000378:metabolism; D006801:Humans; D007375:Interleukin-1; D012175:Retinoblastoma / Q000378:metabolism* / Q000473:pathology; D013110:Sphingosine / Q000031:analogs & derivatives / Q000378:metabolism; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D014407:Tumor Cells, Cultured; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D019925:Cyclin A; D019927:Cyclin E; D004791:Enzyme Inhibitors; D007375:Interleukin-1; C064769:N-acetylsphingosine; D059004:Topoisomerase I Inhibitors; D059005:Topoisomerase II Inhibitors; D014409:Tumor Necrosis Factor-alpha; D000677:Amsacrine; D005047:Etoposide; D013110:Sphingosine; D002166:Camptothecin