title: Polymerase chain reaction-restriction fragment length polymorphism analysis of a short fragment of the cytochrome b gene for identification of flatfish species.
abstract: Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). PCR amplification of the cytochrome b gene using a universal primer together with a primer specifically designed as a part of this study produced a 201-bp fragment in all species analyzed. Digestions of the PCR products with Sau3Al, BsmAl, Rsal, and Mn/l endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.
authors.affiliation: Departamento de Nutrición y Bromatología III (Higiene y Tecnología de los Alimentos), Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Céspedes
authors.forename: T
authors.initials: T
authors.lastname: García
authors.forename: E
authors.initials: E
authors.lastname: Carrera
authors.forename: I
authors.initials: I
authors.lastname: González
authors.forename: B
authors.initials: B
authors.lastname: Sanz
authors.forename: P E
authors.initials: PE
authors.lastname: Hernández
authors.forename: R
authors.initials: R
authors.lastname: Martín
mesh_terms: D000818:Animals; D003573:Cytochrome b Group / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000032:analysis; D005412:Flatfishes / Q000145:classification* / Q000235:genetics; D005513:Food Inspection / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D015183:Restriction Mapping; D013045:Species Specificity
chemical_list: D003573:Cytochrome b Group; D004272:DNA, Mitochondrial