Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR.
A rapid, sensitive, and simple method was developed to detect the sapstain fungi Ophiostoma piceae and O. quercus in stained wood. By using microwave heating for DNA extraction and PCR with internal transcribed spacer-derived-specific primers, detection was feasible within 4 h, even with DNA obtained from a single synnema. This method can easily be extended for the detection of other wood-inhabiting fungi.
Kim
S H
SH
Chair of Forest Products Biotechnology, Department of Wood Science, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4.
Uzunovic
A
A
Breuil
C
C
D001203:Ascomycota / Q000235:genetics* / Q000302:isolation & purification*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014934:Wood
D017931:DNA Primers; D004271:DNA, Fungal