title: Vectors for the expression of tagged proteins in Schizosaccharomyces pombe.
abstract: A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH). Fusion of the carboxyl terminus of a protein to a tag is possible with GFP/EGFP or Pk. Expression of the fusion proteins is controlled by the medium strength mutant version of the regulatable nmt1 promoter.
authors.affiliation: School of Biological Sciences, University of Manchester, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Craven
authors.forename: D J
authors.initials: DJ
authors.lastname: Griffiths
authors.forename: K S
authors.initials: KS
authors.lastname: Sheldrick
authors.forename: R E
authors.initials: RE
authors.lastname: Randall
authors.forename: I M
authors.initials: IM
authors.lastname: Hagan
authors.forename: A M
authors.initials: AM
authors.lastname: Carr
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000235:genetics; D001483:Base Sequence; D003001:Cloning, Molecular; D000939:Epitopes / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D005822:Genetic Vectors / Q000737:chemistry / Q000235:genetics*; D049452:Green Fluorescent Proteins; D006388:Hemagglutinins / Q000235:genetics; D006639:Histidine / Q000235:genetics; D008164:Luminescent Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D012568:Schizosaccharomyces / Q000737:chemistry / Q000166:cytology / Q000235:genetics*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D006388:Hemagglutinins; D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D006639:Histidine