Kinetics and interaction studies between cytochrome c3 and Fe-only hydrogenase from Desulfovibrio vulgaris Hildenborough.
Hydrogenases from Desulfovibrio are found to catalyze hydrogen uptake with low potential multiheme cytochromes, such as cytochrome c3, acting as acceptors. The production of Fe-only hydrogenase from Desulfovibrio vulgaris Hildenborough was improved with respect to the growth phase and media to determine the best large-scale bacteria growth conditions. The interaction and electron transfer from Fe-only hydrogenase to multiheme cytochrome has been studied in detail by both BLAcore and steady-state measurements. The electron transfer between [Fe] hydrogenase and cytochrome c3 appears to be a cooperative phenomenon (h = 1.37). This behavior could be related to the conductivity properties of multihemic cytochromes. An apparent dissociation constant was determined (2 x 10(-7) M). The importance of the cooperativity for contrasting models proposed to describe the functional role of the hydrogenase/cytochrome c3 complex is discussed. Presently, the only determined structure is from [NiFe] hydrogenase and there are no obvious similarities between [NiFe] and [Fe] hydrogenase. Furthermore, no crystallographic data are available concerning [Fe] hydrogenase. The first results on crystallization and X-ray crystallography are reported.
Brugna
M
M
Laboratoire de Bioénergétique et Ingéniérie des Protéines, C.N.R.S., Institut de Biologie Structurale et Microbiologie, Marseille, France.
Giudici-Orticoni
M T
MT
Spinelli
S
S
Brown
K
K
Tegoni
M
M
Bruschi
M
M
D015374:Biosensing Techniques; D018360:Crystallography, X-Ray; D003574:Cytochrome c Group / Q000302:isolation & purification / Q000378:metabolism*; D016969:Desulfovibrio vulgaris / Q000378:metabolism*; D004579:Electron Transport; D006864:Hydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D011487:Protein Conformation; D017433:Protein Structure, Secondary
D003574:Cytochrome c Group; C017165:cytochrome c(3); D006864:Hydrogenase