Semicarbazide-sensitive amine oxidase (SSAO) from human and bovine cerebrovascular tissues: biochemical and immunohistological characterization.
Semicarbazide-sensitive amine oxidase (SSAO) is widely distributed in almost all tissues, especially in vascularized ones. However, its presence in brain microvessels is still controversial. We have investigated the presence of SSAO in human and bovine brain microvessels by biochemical and immunohistological techniques, and we have compared it with SSAO present in meninges from the same species. SSAO metabolizes benzylamine and methylamine in all tissues tested and possibly dopamine and octopamine as well, as shown in competition studies. Kynuramine inhibited the metabolism of benzylamine by SSAO with high affinity in a non-competitive manner. Western-blot analysis rendered a positive staining of a 100 kDa band, in tissues from both species. These results were confirmed by immunohistological studies: the tunica media and intima of the meninges from both species were positively stained, and so was the endothelial layer of microvessels. SSAO was absent in brain parenchyma. These results definitively confirm the presence of SSAO in human and bovine cerebrovascular tissues and they demonstrate for the first time, the presence of this amine oxidase in endothelial cells from microvessels, through biochemical and immunological approaches.
Castillo
V
V
Department of Biochemistry, Universitat Autónoma de Barcelona, Bellaterra, Spain.
Lizcano
J M
JM
Visa
J
J
Unzeta
M
M
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D001808:Blood Vessels / Q000201:enzymology*; D015153:Blotting, Western; D001921:Brain / Q000098:blood supply*; D002417:Cattle; D006801:Humans; D007150:Immunohistochemistry; D007700:Kinetics; D008578:Meninges / Q000201:enzymology; D013379:Substrate Specificity
D006631:Amine Oxidase (Copper-Containing)