A FLASH of insight into cellular chemistry: genetically encoded labels for protein visualization in vivo.
Genetically encoded fluorescent labels, such as green fluorescent protein, make it possible to visualize a protein's natural distribution and environment in living cells. A new approach to protein labeling in living cells has been devised in which a small, membrane-permeable ligand binds with high affinity and specificity to a short peptide motif that can be incorporated into the protein of interest; the ligand becomes brightly fluorescent after binding to the peptide.
Luebke
K J
KJ
Center for Biomedical Inventions, Department of Internal Medicine, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas TX 75235-8573, USA. Luebke@ryburn.swmed.edu
D000818:Animals; D002468:Cell Physiological Phenomena; D003584:Cytological Techniques; D004735:Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis; D015870:Gene Expression; D006801:Humans; D007202:Indicators and Reagents / Q000138:chemical synthesis; D008024:Ligands; D008164:Luminescent Proteins / Q000138:chemical synthesis; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D005456:Fluorescent Dyes; D007202:Indicators and Reagents; D008024:Ligands; D008164:Luminescent Proteins; D011506:Proteins