Effect of environmental pollutants on the C-reactive protein of a freshwater major carp, Catla catla.
C-reactive proteins (CRP) have been affinity purified to electrophoretic homogeneity from the sera of major carp, Catla catla before and after exposure to environmental pollutants. Exposure to these pollutants elevate the levels of circulating CRPs to 2.8-3.5 times the normal values. Kinetic studies of metal intoxication indicate that a unique molecular variant of CRP is present in the serum at the peak level of acute phase induction, and this variant coexists with normal CRPs. Carbohydrate analysis and lectin binding reveals that these CRPs are glycoproteins differing significantly in total carbohydrate contents. Their electrophoretic mobilities in native gel are different but become identical on desialylation and deglycosylation implying that the molecular variants vary in the glycan parts. All these forms of CRP contain two nonidentical subunits of Mr 22 and 29 kDa. Examination of their immunological crossreactivity demonstrate their similarity in overall molecular topology but their differences in the quantitative extent of binding are reflected.
Paul
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Indian Institute of Chemical Biology, Calcutta.
Mandal
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Mandal
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D000818:Animals; D002097:C-Reactive Protein / Q000032:analysis* / Q000302:isolation & purification; D019256:Cadmium Chloride; D002241:Carbohydrates / Q000032:analysis; D002347:Carps; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004785:Environmental Pollutants; D006023:Glycoproteins / Q000032:analysis; D037102:Lectins / Q000378:metabolism; D008627:Mercuric Chloride; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
D002241:Carbohydrates; D004785:Environmental Pollutants; D006023:Glycoproteins; D037102:Lectins; D012967:Sodium Dodecyl Sulfate; D008627:Mercuric Chloride; D002097:C-Reactive Protein; D019256:Cadmium Chloride