Electrophoretic methods for process monitoring and the quality assessment of recombinant glycoproteins.
In many ways electrophoretic techniques appear ideal for quality monitoring of proteins and are thus well suited for the analysis of recombinant glycoproteins. The requirements of high throughput, comparative analysis and resolution of many variants are met by several electrophoretic techniques. A wide variety of such techniques are available to biotechnologists in the rapidly developing area of recombinant glycoproteins. It is the aim of this review to specifically cover recent work which has been applied to the analysis of DNA-derived glycoproteins, both from a process control standpoint and final product validation. All major areas of electrophoresis including sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focusing and techniques utilizing capillary electrophoresis are covered, with emphasis on analysis of glycoforms and oligosaccharide profiles of recombinant glycoproteins. As illustration, actual examples rather than standard glycoproteins are given to indicate the potential and limitations which may be encountered. It is anticipated that this review will prove a useful and practical guide to the latest developments by indicating the relevant merits of different methods.
Taverna
M
M
Laboratoire de Chimie Analytique, Faculté de Pharmacie, Châtenay-Malabry, France. myriam.taverna@cep.u-psud.fr
Tran
N T
NT
Merry
T
T
Horvath
E
E
Ferrier
D
D
D000818:Animals; D004586:Electrophoresis / Q000379:methods*; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis*
D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D010455:Peptides; D011994:Recombinant Proteins