Affinity labelling of the tick-borne encephalitis virus RNA replicase proteins by 4-N-exo-base-substituted photoreactive CTP analogs.
4-N-exo-base-substituted photoreactive analogs of CTP were designed and synthesized. Two flavivirus proteins NS5 and NS3 are shown to be labelled after RNA synthesis in the presence of the analogs, irradiation by UV-light (313 nm) and subsequent [alpha-32P]NTP incorporation.
Morozova
O V
OV
Novosibirsk Institute of Bioorganic Chemistry, Russia. MOV@niboch.nsc.ru
Safronov
I V
IV
Bahvalova
V N
VN
Dobrikov
M I
MI
D000818:Animals; D002460:Cell Line; D003570:Cytidine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D015195:Drug Design; D004669:Encephalitis Viruses, Tick-Borne / Q000201:enzymology*; D007202:Indicators and Reagents; D007668:Kidney; D008958:Models, Molecular; D015394:Molecular Structure; D019920:Photoaffinity Labels / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics*; D020365:RNA Helicases; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D012697:Serine Endopeptidases; D013552:Swine; D014158:Transcription, Genetic; D014466:Ultraviolet Rays; D017361:Viral Nonstructural Proteins / Q000378:metabolism / Q000528:radiation effects; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
D003570:Cytidine Triphosphate; D007202:Indicators and Reagents; D019920:Photoaffinity Labels; D020365:RNA Helicases; D012324:RNA-Dependent RNA Polymerase; D012697:Serine Endopeptidases; D017361:Viral Nonstructural Proteins; C060028:NS3 protein, flavivirus; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases; C096041:NS5 protein, flavivirus