[Delayed determination of ceruloplasmin activity in donors' blood serum after adding chemical stabilizer].
The ceruloplasmin activity in bioassays of donor blood serum, preserved at the temperature of 4-8 degrees C, decreases progressively, beginning from the third day of experiment. Freezing of bioassays is accompanied by similar changes, since this enzyme belongs to cryounstable proteins. It has been found out that the 1-triphenylphosphonomethylnaphthalene bromide in concentrations 1.03-2.58 mmol/l stabilizes ceruloplasmin and preserves its activity unchanged in biologic fluids during 30 days of observation. Having used this fluid we have created a delayed method of determining this enzyme which may be used with the aim of studying metabolic processes in the human body of spacemen, submarine crews, members of arctic, antarctic and other expeditions which cannot be accompanied by a biochemical laboratory.
Iarmol'chuk
H M
HM
Bukovyna State Medical Academy, Chernivtsi.
D001681:Biological Assay; D001782:Blood Donors; D002570:Ceruloplasmin / Q000032:analysis*; D005079:Excipients; D006801:Humans; D009281:Naphthalenes; D009943:Organophosphorus Compounds; D013997:Time Factors
C116570:1-triphenylphosphoniomethylnaphthalene bromide; D005079:Excipients; D009281:Naphthalenes; D009943:Organophosphorus Compounds; D002570:Ceruloplasmin
Vidstrochene vyznachennia aktyvnosti tseruloplazminu v syrovattsi krovi donoriv za dodavannia khimichnoho stabilizatora.