title: Molecular cloning and expression of the early auxin-responsive Aux/IAA gene family in Nicotiana tabacum.
abstract: Early auxin-regulated tobacco cDNAs, belonging to the Aux/IAA gene family have been isolated by screening of a cDNA library prepared from auxin-treated suspension-grown etiolated seedlings of Nicotiana tabacum. The probes used were either RT-PCR fragments or an insert resulting from mRNA differential display selection. All of them possessed the structural features which characterize the Aux/IAA gene products. The auxin response of three distinct Nt-iaa subclasses has been characterized in terms of kinetics, dose-response and specificity as several plant hormones and chemicals have been tested for their ability to alter Nt-iaa mRNA accumulation. Differences of auxin responses have been observed between the Nt-iaa analysed, revealing significant differences of regulation. The effect of the protein synthesis inhibitor cycloheximide suggested that Nt-iaa2.3, Nt-iaa4.3 and strictly related genes can be classified as primary auxin-responsive genes and Nt-iaa28 as a late one. The steady-state mRNA level of these Nt-iaa has also been determined in organs of tobacco plants.
authors.affiliation: Institut des Sciences végétales, CNRS, Gif sur Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Dargeviciute
authors.forename: C
authors.initials: C
authors.lastname: Roux
authors.forename: A
authors.initials: A
authors.lastname: Decreux
authors.forename: F
authors.initials: F
authors.lastname: Sitbon
authors.forename: C
authors.initials: C
authors.lastname: Perrot-Rechenmann
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003513:Cycloheximide; D018076:DNA, Complementary; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D010947:Plants, Toxic; D014026:Nicotiana / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D007210:Indoleacetic Acids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D003513:Cycloheximide