Anti-lipid peroxidation of gomisin J on liver mitochondria and cultured myocardial cells.
OBJECTIVE
To study the influences of gomisin J on lipid peroxidation and calcium paradox.


METHODS
Using two in vitro models of rat liver mitochondria membrane lipid peroxidation (LPO) and cultured myocardial cells.


RESULTS
Gomisin J inhibited Fe2+/ascorbic acid and ADP/NADPH-induced LPO with IC50 (95% confidence limits) 5.5 (4.5-6.7) and 4.7 (2.8-7.8) mumol.L-1, respectively, when cultured myocardial cells preincubated with Ca(2+)-free medium for 2 min were incubated with normal medium containing Ca2+, a marked increase of malondialdehyde (MDA) formation occurred and gomisin J 10 mumol.L-1 protected myocardial cells through decreasing MDA formation.


CONCLUSIONS
Gomisin J inhibits LPO in rat liver mitochondria and protects cultured myocardial cells from being injured by calcium paradox.
Peng
H L
HL
Department of Pharmacology, School of Pharmacy, Shanghai Medical University, China.
Chen
D F
DF
Lan
H X
HX
Zhang
X M
XM
Gu
Z
Z
Jiang
M H
MH
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D002478:Cells, Cultured; D017705:Lignans / Q000494:pharmacology*; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects*; D009206:Myocardium / Q000166:cytology; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D017705:Lignans; D011083:Polycyclic Compounds; D008315:Malondialdehyde; C082091:gomisin J