A fast and inexpensive method for N-terminal fluorescein-labeling of peptides.
A new method has been developed to synthesize fluorescein labeled peptides, compounds of increasing importance in bioorganic chemistry, cell biology, pharmacology, drug targeting and medicinal chemistry. We show, that 4(5)-carboxyfluorescein is much more efficient than the hitherto predominantly utilized reagents 4(5)-carboxyfluorescein-N-succinimidylester and 4(5)-fluoresceinisothiocyanate.
Weber
P J
PJ
Department of Pharmacy, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland.
Bader
J E
JE
Folkers
G
G
Beck-Sickinger
A G
AG
D000595:Amino Acid Sequence; D002234:Carbodiimides; D002623:Chemistry Techniques, Analytical / Q000191:economics / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004126:Dimethylformamide; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis*; D013056:Spectrophotometry, Ultraviolet; D014230:Triazoles
D002234:Carbodiimides; D005452:Fluoresceins; D007202:Indicators and Reagents; D010455:Peptides; D014230:Triazoles; C081611:1,3-diisopropylcarbodiimide; C045132:4-carboxyfluorescein; D004126:Dimethylformamide; C011852:1-hydroxybenzotriazole