[Individual identification of cadaver parts after a bomb explosion using oligonucleotide fingerprinting by (GTG)5].
A blind shell suddenly and unexpectedly exploded, and 20 dismembered human remains were discovered. DNA fingerprint was performed to determine whether the 20 human remains were derived from one person or not. DNA was isolated from each of the remains and digested by the restriction enzyme Hinf I and Hae III and hybridized with the oligonucleotide probe (GTG)5. DNA fingerprint using Hinf I demonstrated the same band pattern in 17 out of the 20 remains. However, in the remaining 3 samples, two novel strange bands were observed. DNA fingerprint using Hae III showed completely identical pattern in all of the remains.
Kondo
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Institut für Rechtsmedizin, Universität Kanazawa, Japan.
Ohshima
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D001753:Blast Injuries / Q000473:pathology*; D016172:DNA Fingerprinting; D005107:Explosions; D006801:Humans; D008297:Male; D015345:Oligonucleotide Probes; D012680:Sensitivity and Specificity
D015345:Oligonucleotide Probes
Individualisierung von Leichenteilen nach einer Bombenexplosion mittels Oligonucleotid-Fingerprinting durch (GTG)5.