title: Toward the antibody-catalyzed chemiluminescence. Design and synthesis of hapten.
abstract: Hapten 4 was synthesized to generate catalytic antibodies triggering chemiluminescence by catalyzing the decomposition of the 1,2-dioxetane 3. The hapten 4 was so designed as to elicit a negatively charged functional group in the antibody combining site to catalyze the beta-elimination of the protecting group in 3 as well as to lock the protecting group into an energetically favorable anti-periplanar conformation.
authors.affiliation: Institute for Chemical Research, Kyoto University, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Sawa
authors.forename: Y
authors.initials: Y
authors.lastname: Imaeda
authors.forename: J
authors.initials: J
authors.lastname: Hiratake
authors.forename: R
authors.initials: R
authors.lastname: Fujii
authors.forename: R
authors.initials: R
authors.lastname: Umeshita
authors.forename: M
authors.initials: M
authors.lastname: Watanabe
authors.forename: H
authors.initials: H
authors.lastname: Kondo
authors.forename: J
authors.initials: J
authors.lastname: Oda
mesh_terms: D017151:Antibodies, Catalytic / Q000737:chemistry / Q000378:metabolism; D015195:Drug Design; D006241:Haptens / Q000737:chemistry* / Q000378:metabolism; D006433:Hemocyanins; D006571:Heterocyclic Compounds / Q000378:metabolism; D006573:Heterocyclic Compounds, 1-Ring; D007202:Indicators and Reagents / Q000378:metabolism; D008163:Luminescent Measurements; D008958:Models, Molecular; D012710:Serum Albumin, Bovine
chemical_list: D017151:Antibodies, Catalytic; D006241:Haptens; D006571:Heterocyclic Compounds; D006573:Heterocyclic Compounds, 1-Ring; D007202:Indicators and Reagents; C079142:1,2-dioxetane; D012710:Serum Albumin, Bovine; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin