Intermediary metabolism of pentachloronitrobenzene in the control and germ-free rat and rat with cannulated bile ducts.
1. Nearly 70% of single oral doses of 14C-labelled pentachloronitrobenzene (PCNB) was excreted in bile within 24 h. 2. The characterized biliary metabolites of PCNB were either mercapturic acid pathway metabolites or catabolites thereof (thiols, methylthiols, S-glucuronides). 3. A major biliary metabolite was S-(aminotetrachlorophenyl)glutathione. 4. Conjugation with glutathione with subsequent catabolism to bis-methylthiotetrachlorobenzene was the major pathway in the control rat. 5. Germ-free experiments showed that only nitro- group displacement occurred, and no nitro- group reduction was detected.
Larsen
G L
GL
US Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, Fargo, ND 58105-5674, USA.
Huwe
J K
JK
Bakke
J E
JE
D000284:Administration, Oral; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001652:Bile Ducts / Q000378:metabolism*; D002247:Carbon Isotopes / Q000032:analysis; D002250:Carbon Radioisotopes / Q000032:analysis; D005260:Female; D005659:Fungicides, Industrial / Q000378:metabolism* / Q000493:pharmacokinetics*; D005856:Germ-Free Life; D008658:Inactivation, Metabolic; D008099:Liver / Q000378:metabolism; D008297:Male; D009578:Nitrobenzenes / Q000378:metabolism* / Q000493:pharmacokinetics*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014556:Urine / Q000737:chemistry
D000814:Aniline Compounds; D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D005659:Fungicides, Industrial; D009578:Nitrobenzenes; C005805:quintozene; C027673:2,3,4,5,6-pentachloroaniline