Synthesis and hybridization property of an oligonucleotide analog containing a 1',3'-di-O-methylene-alpha-D-fructose backbone.
Hydrogen phosphonate monomers of T (thymine) and C(m) (5-methylcytosine) bearing a 1',3'-di-O-methylene-alpha-D-fructose sugar moiety were synthesized and incorporated into an oligonucleotide. Hybridization studies by thermal denaturation experiment indicated that this oligonucleotide did not form a duplex with the complementary RNA target.
Zou
R
R
Gilead Sciences, Inc., Foster City, CA 94404, USA.
Matteucci
M D
MD
D005632:Fructose; D009693:Nucleic Acid Hybridization; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry
D016376:Oligonucleotides, Antisense; D005632:Fructose