Peptidoglycan fine structure of the radiotolerant bacterium Deinococcus radiodurans Sark.
Peptidoglycan from Deinococcus radiodurans was analyzed by high-performance liquid chromatography and mass spectrometry. The monomeric subunit was: N-acetylglucosamine-N-acetylmuramic acid-L-Ala-D-Glu-(gamma)-L-Orn-[(delta)Gly-Gly]-D-Ala-D-Ala. Cross-linkage was mediated by (Gly)2 bridges, and glycan strands were terminated in (1-->6)anhydro-muramic acid residues. Structural relations with the phylogenetically close Thermus thermophilus are discussed.
Quintela
J C
JC
Centro de Biología Molecular "Severo Ochoa," Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Facultad de Ciencias, Campus de Cantoblanco, 28049 Madrid, Spain.
García-del Portillo
F
F
Pittenauer
E
E
Allmaier
G
G
de Pedro
M A
MA
D000595:Amino Acid Sequence; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006095:Gram-Positive Cocci / Q000737:chemistry* / Q000528:radiation effects; D013058:Mass Spectrometry; D015394:Molecular Structure; D010457:Peptidoglycan / Q000737:chemistry*; D011836:Radiation Tolerance; D016963:Thermus thermophilus / Q000737:chemistry
D010457:Peptidoglycan