Hydrodynamics of micropipette aspiration.
The dynamics of human neutrophils during micropipette aspiration are frequently analyzed by approximating these cells as simple slippery droplets of viscous fluid. Here, we present computations that reveal the detailed predictions of the simplest and most idealized case of such a scheme; namely, the case where the fluid of the droplet is homogeneous and Newtonian, and the surface tension of the droplet is constant. We have investigated the behavior of this model as a function of surface tension, droplet radius, viscosity, aspiration pressure, and pipette radius. In addition, we have tabulated a dimensionless factor, M, which can be utilized to calculate the apparent viscosity of the slippery droplet. Computations were carried out using a low Reynolds number hydrodynamics transport code based on the finite-element method. Although idealized and simplistic, we find that the slippery droplet model predicts many observed features of neutrophil aspiration. However, there are certain features that are not observed in neutrophils. In particular, the model predicts dilation of the membrane past the point of being continuous, as well as a reentrant jet at high aspiration pressures.
Drury
J L
JL
Department of Biomedical Engineering, Boston University, Boston, MA 02215 USA.
Dembo
M
M
D055592:Biophysical Phenomena; D001703:Biophysics; D002196:Capillaries / Q000033:anatomy & histology / Q000502:physiology; D018056:Hemorheology / Q000295:instrumentation*; D006801:Humans; D066298:In Vitro Techniques; D008955:Models, Cardiovascular; D009504:Neutrophils / Q000166:cytology* / Q000502:physiology*; D011312:Pressure; D013396:Suction / Q000295:instrumentation*; D013500:Surface Tension; D014783:Viscosity