title: Effect of overproduction of interleukin 5 on dinitrofluorobenzene-induced allergic cutaneous response in mice.
abstract: The effect of overproduction of interleukin (IL) 5 on the allergic cutaneous response was investigated in transgenic mice overexpressing IL-5. Five repeated topical applications of 2, 4-dinitrofluorobenzene (DNFB) to the ears of mice resulted in allergic dermatitis on the ears as well as significant elevation in dinitrophenol-specific IgE antibody and total IgE in the serum in both wild-type and transgenic mice. The development of dermatitis as measured by skin thickness and histopathological changes were potentiated in the transgenic mice. In IL-5 transgenic mice, significant accumulation of eosinophils in skin lesions was observed after five paintings of DNFB, and the magnitudes of eosinophilia and IL-5 messenger RNA expression were significantly higher than in wild-type mice. The dinitrophenol-specific and total IgE in the serum were higher in IL-5 transgenic mice. The late phase reaction of IgE antibody-mediated biphasic cutaneous response was potentiated in IL-5 transgenic mice. The magnitudes of vasopermeability increase by passive cutaneous anaphylaxis, serotonin, and platelet-activating factor were similar in both mice. These results indicate that overproduction of IL-5 resulted in the potentiation of DNFB-induced dermatitis by elevation of IgE production, IgE-mediated allergic late-phase cutaneous reaction, and eosinophilia in the skin lesion.
authors.affiliation: Department of Pharmacology, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Nagai
authors.forename: Y
authors.initials: Y
authors.lastname: Ueda
authors.forename: H
authors.initials: H
authors.lastname: Tanaka
authors.forename: Y
authors.initials: Y
authors.lastname: Hirano
authors.forename: N
authors.initials: N
authors.lastname: Nakamura
authors.forename: N
authors.initials: N
authors.lastname: Inagaki
authors.forename: K
authors.initials: K
authors.lastname: Takatsu
authors.forename: K
authors.initials: K
authors.lastname: Kawada
mesh_terms: D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D017449:Dermatitis, Allergic Contact / Q000276:immunology* / Q000473:pathology; D004139:Dinitrofluorobenzene; D004342:Drug Hypersensitivity; D005260:Female; D006632:Histamine / Q000494:pharmacology; D007073:Immunoglobulin E / Q000502:physiology; D015848:Interleukin-5 / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D010323:Passive Cutaneous Anaphylaxis; D010972:Platelet Activating Factor / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology
chemical_list: D015848:Interleukin-5; D010972:Platelet Activating Factor; D012701:Serotonin; D007073:Immunoglobulin E; D006632:Histamine; D004139:Dinitrofluorobenzene