Assay of ditiocarb sodium and its methyl ester in mouse plasma by column-switching HPLC.
OBJECTIVE
To establish a column-switching high pressure liquid chromatograpy (CSHPLC) with direct injection for determination of ditiocarb sodium (DTC) and its methyl ester (DTC-Me) in mouse plasma.


METHODS
After automated online pretreating column enrichment and clear-up, DTC-Me was flushed and chromatographed on an ordinary reversed-phase analytical column, and monitored by UV at lambda absorption = 276 nm. DTC was methylated before determination.


RESULTS
No potential interfering peaks were identified. The calibration curves of both analytes were linear within the range of 0.1-150 mg.L-1. The correlation coefficients of DTC and DTC-Me were 0.9998 and 0.9995, respectively. The detection limit was 25 micrograms.L-1 and the coefficient of variation in the assay was within 7% for both compounds. The average recoveries were in the range of 95.28 -100.06%. A typical application was presented for mouse after i.v. DTC 50 mg.kg-1.


CONCLUSIONS
The rapid CSHPLC method with direct injection can be used for the study of pharmacokinetics of DTC and DTC-Me.
Tao
X B
XB
Department of Natural and Synthetic Drug Research, College of Pharmacy, Second Military Medical University, Shanghai, China.
Li
W H
WH
D000818:Animals; D002614:Chelating Agents / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004050:Ditiocarb / Q000493:pharmacokinetics*; D051379:Mice
D002614:Chelating Agents; D004050:Ditiocarb