Characterisation of the polysaccharide produced by Acetobacter xylinum strain CR1/4 by light scattering and atomic force microscopy.
The molecular weight of the extracellular polysaccharide (CR1/4) produced by Acetobacter xylinum strain CR1/4 has been shown to be dependent upon growth conditions. Under normal growth conditions a high molecular weight polysaccharide ( > 1 x 10(6) Da) is produced. Maintaining the pH at 5 results in an order of magnitude increase in the total yield of polysaccharide, but also an order of magnitude decrease in molecular weight. Analysis of the CR1/4 polysaccharides by the techniques of atomic force microscopy and static light scattering suggests that they are double helices. In solution the molecules behave as stiff coils with a Kuhn statistical segment length of 325 nm.
Ridout
M J
MJ
Food Biophysics Department, Institute of Food Research, Norwich Laboratory, Colney, UK.
Brownsey
G J
GJ
Gunning
A P
AP
Morris
V J
VJ
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005285:Fermentation; D041822:Gluconacetobacter xylinus / Q000737:chemistry* / Q000254:growth & development* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D008027:Light; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry*; D012542:Scattering, Radiation
D011135:Polysaccharides, Bacterial