title: [Studies on purging leukemic cells by photosensitizer PSD-007 laser photoradiation in vitro].
abstract: The sensitivity to photosensitization mediated by the hematoporphyrin photosensitizer PSD-007 of acute promyelocytic leukemic cell line (HL-60) was compared with normal human hemopoietic progenitor cells. The results showed that the leukemic cells were more sensitive. After being treated with 10 micrograms.ml-1 PSD-007 followed by 2J.cm-2 copper vapor laser light irradiation, the clonogenic leukemic (HL-60) cells were reduced 98%, but the survival rate of human granulocyte-macrophage colony-forming units (CFU-GM) was 40 +/- 8%. Mixing of normal human marrow cells with leukemic (HL-60) cells (ratio 100:1) did not interfere with elimination of tumor cells. The ultrastructure changes of HL-60 cells treated by laser photoradiation was observed under the trans-electronic microscope. The mitochondria, endoplasmic reticulum and cell membrane were involved. This means that the cell biomembrane is the main target to be attacked. It is considered that PSD-007-laser photoradiation therapy is efficient for killing leukemic cells.
authors.affiliation: Department of Hematology, Xiangya Hospital, Hunan Medical University, Changsha.
authors.forename: X
authors.initials: X
authors.lastname: Liang
authors.forename: Z
authors.initials: Z
authors.lastname: Qi
authors.forename: P
authors.initials: P
authors.lastname: Cao
authors.forename: J
authors.initials: J
authors.lastname: Xiao
authors.forename: G
authors.initials: G
authors.lastname: Tang
authors.forename: X
authors.initials: X
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Lai
mesh_terms: D000328:Adult; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D018922:HL-60 Cells / Q000187:drug effects* / Q000648:ultrastructure; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006414:Hematoporphyrin Photoradiation; D006801:Humans; D017319:Photosensitizing Agents / Q000494:pharmacology*
chemical_list: D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative