Structural models of the bovine papillomavirus E5 protein.
The bovine papillomavirus E5 protein is thought to be a type II integral membrane protein that exists as a disulfide-linked homodimer in transformed cells. Polarized-infrared measurements show that the E5 dimer in membrane bilayers is largely alpha-helical and has a transmembrane orientation. Computational searches of helix-helix conformations reveal two possible low-energy dimer structures. Correlation of these results with previous mutagenesis studies on the E5 protein suggests how the E5 dimer may serve as a molecular scaffold for dimerization and ligand-independent activation of the PDGF-beta receptor. We propose that on each face of the E5 dimer a PDGF-beta receptor molecule interacts directly with Gln17 from one E5 monomer and with Asp33 from the other E5 monomer. This model accounts for the requirement of Gln17 and Asp33 for complex formation and explains genetic results that dimerization of the E5 protein is essential for cell transformation.
Surti
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Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut, USA.
Klein
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Aschheim
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DiMaio
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Smith
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D000465:Algorithms; D000818:Animals; D002417:Cattle; D003198:Computer Simulation; D019281:Dimerization; D007700:Kinetics; D008958:Models, Molecular; D009856:Oncogene Proteins, Viral / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D009856:Oncogene Proteins, Viral; C054304:oncogene protein E5, Bovine papillomavirus type 1; D020797:Receptor, Platelet-Derived Growth Factor beta; D017479:Receptors, Platelet-Derived Growth Factor