Estimating the persistence length of a worm-like chain molecule from force-extension measurements.
We describe a simple computation of the worm-like chain model and obtain the corresponding force-versus-extension curve. We propose an improvement to the Marko and Siggia interpolation formula of Bustamante et al (Science 1994, 265:1599-1600) that is useful for fitting experimental data. We apply it to the experimental elasticity curve of single DNA molecules. Finally, we present a tool to study the agreement between the worm-like chain model and experiments.
Bouchiat
C
C
LPT, ENS, Laboratoire propre du CNRS, Paris, France.
Wang
M D
MD
Allemand
J
J
Strick
T
T
Block
S M
SM
Croquette
V
V
D055592:Biophysical Phenomena; D001703:Biophysics; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D004548:Elasticity; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D013816:Thermodynamics
D004247:DNA