Identification of olfactory receptor mRNA sequences from the rat olfactory bulb glomerular layer.
In situ hybridization has demonstrated mRNA for olfactory receptors (OR) in the axon terminals of olfactory receptor neurons. Neurons that express the same OR appear to send their axons to two stereotyped glomeruli in the olfactory bulb (OB). Based on these observations, we tested the feasibility of using RT-PCR to isolate and sequence OR mRNA from small samples of the rat OB glomerular layer. Biomagnetic mRNA isolation followed by RT-PCR yielded partial sequences for 21 novel members of the OR family. The results suggest that the topography of OR mRNA can be mapped across the OB, to study synaptic specificity and odor representation in the olfactory system.
Singer
M S
MS
Section of Neurobiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Hughes
T E
TE
Shepherd
G M
GM
Greer
C A
CA
D000818:Animals; D019204:GTP-Binding Proteins / Q000502:physiology; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D009830:Olfactory Bulb / Q000166:cytology*; D018034:Olfactory Receptor Neurons / Q000737:chemistry* / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018035:Receptors, Odorant / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D012333:RNA, Messenger; D018035:Receptors, Odorant; D019204:GTP-Binding Proteins