title: Differential regulation of enolase during anaerobiosis in maize.
abstract: It was reported previously that enolase enzyme activity and ENO1 transcript levels are induced by anaerobic stress in maize (Zea mays). Here we show that not all isoforms of maize enolase are anaerobically induced. We cloned and sequenced a second enolase cDNA clone (pENO2) from maize. Sequence analysis showed that pENO2 shares 75.6% nucleotide and 89.5% deduced amino acid sequence identity with pENO1 and is encoded by a distinct gene. Expression of ENO2 is constitutive under aerobic conditions, whereas ENO1 levels are induced 10-fold in maize roots after 24 h of anaerobic treatment. Western-blot analysis and N-terminal sequencing of in vivo-labeled maize roots identified two major proteins selectively synthesized upon anaerobic stress as isozymes of enolase. We describe the expression of enolase in maize roots under anaerobic stress.
authors.affiliation: Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
authors.forename: S K
authors.initials: SK
authors.lastname: Lal
authors.forename: C
authors.initials: C
authors.lastname: Lee
authors.forename: M M
authors.initials: MM
authors.lastname: Sachs
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010751:Phosphopyruvate Hydratase / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D018517:Plant Roots / Q000201:enzymology; D017386:Sequence Homology, Amino Acid; D003313:Zea mays / Q000201:enzymology* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018744:DNA, Plant; D007527:Isoenzymes; D010751:Phosphopyruvate Hydratase