title: Impact of hepatitis G virus co-infection on the course of hepatitis C virus infection before and after liver transplantation.
abstract: OBJECTIVE
  Hepatitis G virus (HGV), a new RNA virus that is parenterally transmitted, has frequently been found in patients with chronic hepatitis C (HCV) infection but its role in chronic liver disease is unknown. The purpose of this study was to determine the prevalence of HGV infection in transplantation patients infected with hepatitis C and to assess the impact of HGV co-infection on the course of HCV infection after liver transplantation.
  
  
  METHODS
  Eighty-nine liver transplantation recipients with persistent hepatitis C viremia detected by polymerase chain reaction (PCR) were evaluated. Serum samples were tested before and after liver transplantation for HGV RNA by two different PCR methods: LC assay (Abbott Laboratories) and an RT-PCR procedure which we developed using the silica gel technique for extraction of the HGV RNA. E2 antibodies were detected before orthotopic liver transplantation by an EIA-test. HCV RNA was quantified by branched DNA assay, and HCV genotype was determined. A mean of nine liver biopsy specimens were examined for each patient and the severity of the lesions was compared in HCV-positive patients with or without HGV co-infection.
  
  
  RESULTS
  The concordance between the two HGV RNA detection methods was excellent and the reproducibility of our RT-PCR procedure was confirmed. The prevalence of pretransplantation and posttransplantation HGV infection was 11% and 19%, respectively. Pretransplantation HGV infection was positively correlated with posttransplantation HGV infection (p<0.001). Before transplantation the E2 antibodies seroprevalence was 34%. Seven patients became HGV RNA positive after transplantation, but all of them were negative for E2 antibodies. Among the patients who remained RNA negative after liver transplantation, 40% were positive for E2 antibodies (p=0.04). Pretransplantation clinical features (except AST mean value) were not different in patients with HCV and HGV co-infection and those with HCV only. After a mean follow-up of 34 months (range: 6 to 70), 67/89 (75%) patients developed histological features of recurrent hepatitis but the frequency of the occurrence of graft hepatitis was not different between HGV/HCV co-infected patients and those with HCV alone (p=0.89). The mean interval from orthotopic liver transplantation to recurrence was 12.2 months (range: 3-63), which was not different for HVG/HVC-co-infected patients and HCV-infected patients. The histological severity of posttransplantation liver disease, and the graft and patient survival were not different for patients with and without HGV co-infection.
  
  
  CONCLUSIONS
  Our results suggest the general persistence of HGV infection after liver transplantation, but HGV co-infection did not appear to influence the posttransplantation course of HCV infection. Before transplantation the prevalence of E2 antibodies was 34%, and our data clearly indicate that E2 antibodies were protective against HGV infection.
authors.affiliation: Hepatology Unit Hôtel-Dieu, and INSERM U 271 Lyon, France.
authors.forename: T
authors.initials: T
authors.lastname: Bizollon
authors.forename: S
authors.initials: S
authors.lastname: Guichard
authors.forename: S N
authors.initials: SN
authors.lastname: Ahmed
authors.forename: P
authors.initials: P
authors.lastname: Chevallier
authors.forename: C
authors.initials: C
authors.lastname: Ducerf
authors.forename: M
authors.initials: M
authors.lastname: Sepetjan
authors.forename: J
authors.initials: J
authors.lastname: Baulieux
authors.forename: C
authors.initials: C
authors.lastname: Trepo
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D018067:Flaviviridae / Q000302:isolation & purification*; D005602:France / Q000453:epidemiology; D006526:Hepatitis C / Q000150:complications / Q000601:surgery*; D006525:Hepatitis, Viral, Human / Q000150:complications / Q000453:epidemiology / Q000601:surgery*; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D012008:Recurrence; D015996:Survival Rate