Cholesterol induced variations of membrane dynamics related to the induction of apoptosis in mouse thymocytes.
OBJECTIVE
To evaluate the involvement of cholesterol induced variations of membrane dynamics in mouse thymocyte apoptosis.


METHODS
Membranes of thymocytes of RK mice were enriched with cholesterol using methyl-beta-cyclodextrins as carriers. Spontaneous apoptosis was compared with apoptosis induced either by X-irradiation, by treatment with dexamethasone (DEX), and by phorbol-12-myristate-13-acetate (PMA). Apoptotic cells were quantified by means of flow cytofluorometry.


RESULTS
Small amounts of incorporated cholesterol enhance the cellular sensitivity for spontaneous apoptosis whereas larger amounts of incorporated cholesterol protect against spontaneous apoptosis and apoptosis induced by irradiation, DEX, or PMA.


CONCLUSIONS
Cholesterol exerts specific rigidity effects on lipid membranes which have been shown to be involved in thymocyte apoptosis. The general effect of higher concentrations of cholesterol protecting against apoptosis hints towards a central protective mechanism. This study believes that either cholesterol paralyses great parts of the cell metabolism or that the apoptotic chain reaction is interrupted at a central point due to protection of membrane lipid regions from oxidative stress.
Härtel
S
S
Institute of Experimental Physics (Biophysics), University of Bremen, Germany.
Ojeda
F
F
Diehl
H
H
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002273:Carcinogens / Q000494:pharmacology; D002452:Cell Count; D002784:Cholesterol / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D053938:DNA Fragmentation; D003907:Dexamethasone / Q000494:pharmacology; D005453:Fluorescence; D005938:Glucocorticoids / Q000494:pharmacology; D008560:Membrane Fluidity / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008862:Microsomes, Liver / Q000737:chemistry / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D013552:Swine; D013601:T-Lymphocytes / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D013997:Time Factors
D002273:Carcinogens; D005938:Glucocorticoids; D003907:Dexamethasone; D002784:Cholesterol; D013755:Tetradecanoylphorbol Acetate