Mouse homolog of the yeast origin recognition complex subunit ORC1 and chromosomal localization of the cognate mouse gene Orc1.
ORC1 encodes a subunit of the eukaryotic origin recognition complex in yeast, which has been shown to play a crucial role in chromosomal DNA replication and transcriptional silencing. We have identified a mouse protein closely related to Saccharomyces cerevisiae ORC1 as well as to the human, Xenopus and Drosophila homologs. MmORC1 has putative cyclin-dependent phosphorylation sites and a nucleotide triphosphate-binding motif. We have mapped the gene locus of the murine Orc1 gene to chromosome 4, band D. Conservation of structures among members of the ORC1-related proteins suggests that these proteins play a key role in the intiation of DNA replication in all eukaryotes.
Zisimopoulou
P
P
Institute of Biochemistry, Biozentrum, University of Würzburg, Germany.
Staib
C
C
Nanda
I
I
Schmid
M
M
Grummt
F
F
D000595:Amino Acid Sequence; D000818:Animals; D002874:Chromosome Mapping; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex; D017386:Sequence Homology, Amino Acid
D004268:DNA-Binding Proteins; C497228:Orc1 protein, mouse; D051738:Origin Recognition Complex