title: Isolation of a multidrug resistance inhibitor from Aconitum pseudo-laeve var. erectum.
abstract: To overcome multidrug resistance (MDR) in cancer chemotherapy, we prepared various plant extracts and searched for a component which is effective for inhibition of MDR. MDR inhibition activity was determined by measuring cytotoxicity to MDR cells using multidrug resistant human fibrocarcinoma KB V20C, which is resistant to 20 nM vincristine and expresses high level of mdr1 gene. Of various plant extracts, the MeOH extract of the root of Aconitum pseudo-laeve var. erectum was found to have potent inhibitory activity on MDR. The bioassay-guided fractionation of the MeOH extract of the plant led to the isolation of an alkaloid, lycaconitine, as an active principle. And the IC50 of lycaconitine for KB V20C cells was 74 micrograms/ml.
authors.affiliation: College of Pharmacy, Sung Kyun Kwan University, Suwon, Korea.
authors.forename: D K
authors.initials: DK
authors.lastname: Kim
authors.forename: H Y
authors.initials: HY
authors.lastname: Kwon
authors.forename: K R
authors.initials: KR
authors.lastname: Lee
authors.forename: D K
authors.initials: DK
authors.lastname: Rhee
authors.forename: O P
authors.initials: OP
authors.lastname: Zee
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D018432:Drug Resistance, Multiple / Q000502:physiology*; D006801:Humans; D010946:Plants, Medicinal / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D000972:Antineoplastic Agents, Phytogenic; C101813:lycaconitine