[Study on the cytotoxic and DNA damaging effects in oral mucosal fibroblasts by areca nut extract].
An aqueous areca nut extract (ANE) was tested for its cytotoxic effects on cultured human embryo oral mucosal fibroblasts (HE-OMF) in vitro by using the trypan blue and thiazolyl blue (MTT) assay. The ANE decreases the cell survival rate in a dose-dependent manner (P < 0.05). So was the extract in inducing damage on the cellular DNA of HE-OMF in vitro examined by the nick translation assay. The increase in counts per minute (CPM) values was significant (P < 0.05) for comparing all four concentration groups tested, The results suggests that aqueous ANE is highly cytotoxic and capable to induce DNA damage on cultured HE-OMF. It may have potential carcinogenic effect on the oral mucosal membrane of whom habitually chewing the areca nut frequently for quite a long time. Futher study is required to illustrate the detail process and study the mechanism of these effects.
Fang
C
C
Oral Faculty, Hunan Medical University, Changsha.
Liu
S
S
Sheng
Z
Z
Li
Z
Z
Tang
F
F
D001114:Areca / Q000633:toxicity*; D015197:Carcinogenicity Tests; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects*; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D009061:Mouth Mucosa / Q000166:cytology*; D010936:Plant Extracts / Q000633:toxicity; D010946:Plants, Medicinal
D010936:Plant Extracts