Comparison of ribotyping and arbitrarily primed PCR for molecular typing of Salmonella enterica and relationships between strains on the basis of these molecular markers.
Arbitrarily primed PCR (AP-PCR) using a discriminatory 10-mer primer and an automated EcoRI ribotyping technique (Riboprinter) were compared for their ability to discriminate between 100 serovars of Salmonella, including multiple isolates representing Salm. Enteritidis PT4 and Salm. Typhimurium DT104. Profiles generated by each method were subjected to numerical analysis using GelCompar software, resulting in the construction of phylogenetic trees and calculation of Simpson's numerical index of diversity (DI). Both methods were highly discriminatory for isolates of Salmonella (Ribotype DI = 0.990, AP-PCR DI = 0.997) with EcoRI ribotyping proving more discriminatory than AP-PCR for isolates of Typhimurium DT104. The population structure was found to be clonal by numerical analysis of markers generated by both methods with serovars being polyphyletic in some cases and grouped in a single cluster in others. No absolute correlation was observed in the relationships between strains formed on the basis of ribo- and AP-PCR markers and serological characteristics.
Hilton
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AC
Institute of Public & Environmental Health, University of Birmingham, Edgbaston, UK. A.C.Hilton@bham.ac.uk
Penn
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CW
D015373:Bacterial Typing Techniques; D005819:Genetic Markers; D016133:Polymerase Chain Reaction / Q000379:methods*; D012329:RNA, Bacterial / Q000145:classification; D012335:RNA, Ribosomal / Q000145:classification; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics
D005819:Genetic Markers; D012329:RNA, Bacterial; D012335:RNA, Ribosomal