title: Successful preimplantation genetic diagnosis is related to the number of available cumulus-oocyte complexes.
abstract: The inheritance pattern of monogenic inheritable disorders influences the proportion of unaffected embryos after preimplantation genetic diagnosis (PGD). We aimed to investigate the influence of the number of cumulus-oocyte complexes (COC) on the outcome after PGD. Eighty-four cycles of 47 couples were included in our analysis. All couples were at risk of transmitting autosomal recessive, autosomal dominant, X-linked single gene disorders or sexaneuploidies to their offspring. One PGD cycle was carried out for a Yq-deletion of the man. The correlation between the numbers of COC and biopsied embryos and between the numbers of COC and unaffected embryos was highly significant (P <0.05). A pregnancy occurred in 15 cycles and a minimum of six COC were needed to achieve a pregnancy. Thirteen pregnancies were observed in cycles with at least 9 COC. The transfer rate and number of transferred embryos per cycle in the subgroups with <9 COC and > or =9 COC were significantly higher in the latter. Although pregnancy rates did not differ significantly between the two subgroups (probably due to the low number of pregnancies), our data indicate that it is justifiable to cancel PGD cycles in which it is expected that <6 COC will be retrieved and that the couple should be informed about the poor prognosis if <9 COC are retrieved.
authors.affiliation: Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University, Belgium.
authors.forename: M
authors.initials: M
authors.lastname: Vandervorst
authors.forename: I
authors.initials: I
authors.lastname: Liebaers
authors.forename: K
authors.initials: K
authors.lastname: Sermon
authors.forename: C
authors.initials: C
authors.lastname: Staessen
authors.forename: A
authors.initials: A
authors.lastname: De Vos
authors.forename: H
authors.initials: H
authors.lastname: Van de Velde
authors.forename: E
authors.initials: E
authors.lastname: Van Assche
authors.forename: H
authors.initials: H
authors.lastname: Joris
authors.forename: A
authors.initials: A
authors.lastname: Van Steirteghem
authors.forename: P
authors.initials: P
authors.lastname: Devroey
mesh_terms: D003550:Cystic Fibrosis / Q000235:genetics; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D030342:Genetic Diseases, Inborn / Q000175:diagnosis*; D008040:Genetic Linkage; D006801:Humans; D007246:Infertility / Q000628:therapy; D008607:Intellectual Disability / Q000235:genetics; D007713:Klinefelter Syndrome / Q000235:genetics; D008297:Male; D009136:Muscular Dystrophies / Q000235:genetics; D009865:Oocytes / Q000502:physiology*; D006080:Ovarian Follicle / Q000166:cytology*; D011247:Pregnancy; D011296:Prenatal Diagnosis; D012729:Sex Chromosome Aberrations; D014960:X Chromosome; D014998:Y Chromosome