Effects of N-acetylcysteine and 2,3-dimercaptosuccinic acid on lead induced oxidative stress in rat lenses.
Lead (Pb) is known to disrupt the pro-oxidant/anti-oxidant balance of tissues which leads to biochemical and physiological dysfunction. The present study investigated the effects of exposure on the redox status of the lenses of Fisher 344 rats and examined whether antioxidant or chelator administration reversed these changes. Animals were given 5 weeks of 2000 ppm Pb exposure followed by 1 week of either antioxidant, chelator or distilled water administration. Glutathione (GSH) and cysteine (CYS) levels decreased in the Pb-exposed group. N-acetylcysteine or 2,3-dimercaptopsuccinic acid (Succimer) supplementation following Pb intoxication resulted in increases in the GSH and CYS levels. Protein bound glutathione (PSSG) and cysteine (PSSC) increased following Pb exposure. In the Succimer-treated animals, the PSSG decreased significantly. The glutathione disulfide (GSSG) levels remained unchanged. Malondialdehyde (MDA) levels, a major lipid peroxidation byproduct, increased following Pb exposure and decreased following Succimer treatment. Our results suggest that antioxidant supplementation, as well as chelation, following Pb exposure may enhance the reductive status of lenses.
Neal
R
R
Department of Chemistry, University of Missouri-Rolla, 65409-0010, USA.
Cooper
K
K
Gurer
H
H
Ercal
N
N
D000111:Acetylcysteine / Q000494:pharmacology*; D000818:Animals; D002614:Chelating Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000378:metabolism; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009942:Organometallic Compounds / Q000097:blood / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D011916:Rats, Inbred F344; D004113:Succimer / Q000494:pharmacology*
D002614:Chelating Agents; D016166:Free Radical Scavengers; D009942:Organometallic Compounds; D008315:Malondialdehyde; D004113:Succimer; D005978:Glutathione; D003545:Cysteine; C008261:lead acetate; D019803:Glutathione Disulfide; D000111:Acetylcysteine