Radio-imaging for quantitative autoradiography in biology.
We present here an overview of new in vitro and ex vivo radio-imaging systems developed to overcome the limitations of films and emulsions currently used in histological autoradiography experiments. The shortcomings of films for quantitative studies are first introduced. Principles and performances of each family of imagers are discussed and illustrated in various biological contexts. Finally, perspectives of development including nonradioactive labeling techniques are briefly presented.
Charon
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Institut de Physique Nucléaire d'Orsay and Groupe Modélisation Interfaces Physique Biologie, Université Diderot, Paris, France. charon@ipno.in2p3.fr
Lanièce
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Tricoire
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D001345:Autoradiography / Q000379:methods*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007553:Isotope Labeling; D008163:Luminescent Measurements; D011856:Radiographic Image Enhancement / Q000295:instrumentation; D011857:Radiographic Image Interpretation, Computer-Assisted; D011877:Radionuclide Imaging / Q000295:instrumentation*; D014962:X-Ray Film