title: Fine structure and morphogenesis of Borna disease virus.
abstract: Borna disease virus (BDV), a negative nonsegmented single-stranded RNA virus, has not been fully characterized morphologically. Here we present what is to our knowledge the first data on the fine ultrastructure and morphogenesis of BDV. The supernatant of MDCK cells persistently infected with BDV treated with n-butyrate contained many virus-like particles and more BDV-specific RNA than that of untreated samples. The particles were spherical, enveloped, and approximately 130 nm in diameter; had spikes 7 nm in length; and reacted with BDV p40 antibody. A thin nucleocapsid, 4 nm in width, was present peripherally in contrast to the thick nucleocapsid of hemagglutinating virus of Japan. The BDV particles reproduced by budding on the cell surface.
authors.affiliation: Department of Microbiology, Takatsuki, Osaka 569-8686, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Kohno
authors.forename: T
authors.initials: T
authors.lastname: Goto
authors.forename: T
authors.initials: T
authors.lastname: Takasaki
authors.forename: C
authors.initials: C
authors.lastname: Morita
authors.forename: T
authors.initials: T
authors.lastname: Nakaya
authors.forename: K
authors.initials: K
authors.lastname: Ikuta
authors.forename: I
authors.initials: I
authors.lastname: Kurane
authors.forename: K
authors.initials: K
authors.lastname: Sano
authors.forename: M
authors.initials: M
authors.lastname: Nakai
mesh_terms: D000818:Animals; D001891:Borna disease virus / Q000302:isolation & purification / Q000648:ultrastructure*; D002460:Cell Line; D004285:Dogs; D009024:Morphogenesis; D012367:RNA, Viral / Q000032:analysis; D014771:Virion / Q000302:isolation & purification
chemical_list: D012367:RNA, Viral