The tomato Mi-1 gene confers resistance to both root-knot nematodes and potato aphids.
Mi-1, a Lycopersicon peruvianum gene conferring resistance to the agricultural pests, root-knot nematodes, and introgressed into tomato, has been cloned using a selective restriction fragment amplification based strategy. Complementation analysis of a susceptible tomato line with a 100 kb cosmid array yielded a single cosmid clone capable of conferring resistance both to the root-knot nematode Meloidogyne incognita and to an unrelated pathogen, the potato aphid Macrosiphum euphorbiae. This resistance was stable. The Mi-1 gene encodes a protein sharing structural features with the nucleotide-binding site leucine-rich repeat-containing type of plant resistance genes.
Vos
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Keygene N.V., Agro Business Park, Wageningen, The Netherlands. keygene@euronet.nl
Simons
G
G
Jesse
T
T
Wijbrandi
J
J
Heinen
L
L
Hogers
R
R
Frijters
A
A
Groenendijk
J
J
Diergaarde
P
P
Reijans
M
M
Fierens-Onstenk
J
J
de Both
M
M
Peleman
J
J
Liharska
T
T
Hontelez
J
J
Zabeau
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001042:Aphids; D003001:Cloning, Molecular; D003360:Cosmids; D017343:Genes, Plant; D005816:Genetic Complementation Test; D018551:Solanum lycopersicum / Q000235:genetics* / Q000469:parasitology; D008969:Molecular Sequence Data; D009348:Nematoda; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011198:Solanum tuberosum / Q000469:parasitology*
C117349:MI-1 protein, Lycopersicon esculentum; D010940:Plant Proteins