Laser ablation of persistent twist cells in Drosophila: muscle precursor fate is not segmentally restricted.
In Drosophila the precursors of the adult musculature arise during embryogenesis. These precursor cells have been termed Persistent Twist Cells (PTCs), as they continue to express the transcription factor Twist after that gene ceases expression elsewhere in the mesoderm. In the larval abdomen, the PTCs are associated with peripheral nerves in stereotypic ventral, dorsal, and lateral clusters, which give rise, respectively, to the ventral, dorsal, and lateral muscle fiber groups of the adult. We tested the developmental potential of the PTCs by using a microbeam laser to ablate specific clusters in larvae. We found that the ablation of a single segmental PTC cluster does not usually result in the deletion of the corresponding adult fibers of that segment. Instead, normal or near normal numbers of adult fibers can form after the ablation. Examination of pupae following ablation showed that migrating PTCs from adjacent segments are able to invade the affected segment, replenishing the ablated cells. However, the ablation of homologous PTCs in multiple segments does result in the deletion of the corresponding adult muscle fibers. These data indicate that the PTCs in an abdominal segment can contribute to the formation of muscle fibers in adjacent abdominal segments, and thus are not inherently restricted to the formation of muscle fibers within their segment of origin.
Farrell
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Department of Molecular, Cellular and Developmental Biology, Yale University, PO Box 208103, New Haven, CT 06520, USA.
Keshishian
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D000005:Abdomen / Q000528:radiation effects; D000818:Animals; D002452:Cell Count; D002465:Cell Movement; D004330:Drosophila / Q000196:embryology*; D006651:Histocytochemistry; D007814:Larva / Q000166:cytology / Q000528:radiation effects*; D053685:Laser Therapy; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D011679:Pupa / Q000166:cytology