Cloning and characterization of Sel-1l, a murine homolog of the C. elegans sel-1 gene.
The Notch signaling pathway regulates specification and proliferation in a variety of cell lineages in invertebrates and vertebrates. We have cloned a murine homolog of SEL-1, a key negative regulator of the Notch pathway in Caenorhabditis elegans. Murine SEL-1L (mSEL-1L) protein exhibits a high degree of similarity to SEL-1, including a signal peptide and the C-terminal region required for SEL-1 function in C. elegans. This mammalian homolog of sel-1 is widely expressed in adult mouse and human tissues, with particularly high levels in the pancreas. RNA in situ analysis of developing mouse embryos indicates that mSEL-1L is moderately expressed throughout the neural tube and dorsal root ganglia, with particularly high levels in the floor plate of the neural tube beginning at E10.5 and increasing at E11.5. Expression is high at E14.5 and E17.5 in the acini of the pancreas, and moderate in the epithelial cells of the gut villi. We localized the SEL-1L protein to the cytosol, possibly in intracellular vesicles, in a beta-islet-derived tumor cell line (RinM).
Donoviel
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Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada. donoviel@mshri.on.ca
Donoviel
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Fan
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Hadjantonakis
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Bernstein
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D000328:Adult; D000595:Amino Acid Sequence; D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003600:Cytosol / Q000378:metabolism; D005314:Embryonic and Fetal Development / Q000235:genetics*; D020224:Expressed Sequence Tags; D005326:Fetal Proteins / Q000096:biosynthesis* / Q000235:genetics; D005727:Ganglia, Spinal / Q000196:embryology / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D015801:Helminth Proteins / Q000235:genetics*; D006801:Humans; D017403:In Situ Hybridization; D047908:Intracellular Signaling Peptides and Proteins; D007515:Islets of Langerhans / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics* / Q000502:physiology*; D051379:Mice / Q000235:genetics*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000096:biosynthesis* / Q000235:genetics; D009420:Nervous System / Q000196:embryology / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000473:pathology; D014176:Protein Biosynthesis; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors; D014407:Tumor Cells, Cultured
D029742:Caenorhabditis elegans Proteins; D005326:Fetal Proteins; D015801:Helminth Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C497761:NOTCH1 protein, human; D009419:Nerve Tissue Proteins; C497762:Notch1 protein, mouse; D021382:Protein Sorting Signals; D011506:Proteins; D012333:RNA, Messenger; D051881:Receptor, Notch1; D011956:Receptors, Cell Surface; D011993:Recombinant Fusion Proteins; C101221:SEL-1 protein, C elegans; C117384:SEL1L protein, human; C492169:Sel1h protein, mouse; D014157:Transcription Factors