title: In vitro studies on the use of clay, clay minerals and charcoal to adsorb bovine rotavirus and bovine coronavirus.
abstract: Rotaviruses are the leading cause and coronaviruses are the major contributors of acute gastroenteritis in the young of various mammalian and avian species. Despite numerous trials and decades of research, vaccines have limited efficacy particularly for calves. As an alternative method of controlling infection, we have investigated broad spectrum antiviral agents that are not discriminatory among various viruses. This report involves testing a variety of adsorbent agents including charcoal, clay, and clay minerals to adsorb rotavirus and coronavirus in vitro. Results revealed that all the adsorbent agents had good to excellent capability of adsorbing rotavirus and excellent capability of adsorbing coronavirus. Percent adsorptions ranged from 78.74% to 99.89% for rotavirus and 99.99% for coronavirus; while sand (negative control) was < 0.01%. A high affinity binding was present as determined by a low percent desorption (0.06-3.09%). However, the adsorbent bound virus complex retained, and may have actually enhanced, infectivity.
authors.affiliation: Department of Veterinary Pathobiology and Public Health, College of Veterinary Medicine, Texas A&M University, College Station 77845, USA.
authors.forename: K J
authors.initials: KJ
authors.lastname: Clark
authors.forename: A B
authors.initials: AB
authors.lastname: Sarr
authors.forename: P G
authors.initials: PG
authors.lastname: Grant
authors.forename: T D
authors.initials: TD
authors.lastname: Phillips
authors.forename: G N
authors.initials: GN
authors.lastname: Woode
mesh_terms: D000327:Adsorption; D000538:Aluminum Silicates; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000628:therapy; D002606:Charcoal; D000077215:Clay; D018352:Coronavirus Infections / Q000628:therapy / Q000662:veterinary; D017938:Coronavirus, Bovine / Q000302:isolation & purification*; D008903:Minerals; D012401:Rotavirus / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000628:therapy / Q000662:veterinary
chemical_list: D000538:Aluminum Silicates; D008903:Minerals; D002606:Charcoal; D000077215:Clay