[Formation of inositol triphosphate in lymphocytes of rat spleen in the early stages after x-irradiation].
Stimulation of 32P-phosphoinositides turnover and phosphatidylinositol-4,5-biphosphate hydrolysis is observed 6 h after animals X-ray irradiation in a dose 1 Gy. Both mitogenic lectins LPS and PHA (10 mg/ml), but not 0.5 microM A23187 induce inositol phosphates release from endogenous [3H]inositol--phospholipids in lymphocytes. The level of inositol triphosphate is increased within 1-6 h and decreased in 12 h after irradiation. 45Ca2+ influx through lymphocytes plasma membrane is not changed within 3 h, but increases greatly 12 h after exposure. Data obtained indicate that IP3 formation is an early signal for radiation-induced changes in calcium homeostasis.
Matyshevs'ka
O P
OP
Taras Shevchenko Kyiv University.
D000818:Animals; D002118:Calcium / Q000097:blood*; D006706:Homeostasis / Q000528:radiation effects; D006868:Hydrolysis; D007295:Inositol Phosphates / Q000096:biosynthesis*; D008214:Lymphocytes / Q000378:metabolism / Q000528:radiation effects*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D051381:Rats; D013154:Spleen / Q000276:immunology / Q000378:metabolism / Q000528:radiation effects*
D007295:Inositol Phosphates; D019269:Phosphatidylinositol 4,5-Diphosphate; D010761:Phosphorus Radioisotopes; D002118:Calcium
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