[A preliminary study on p53 gene expression and infection of human papilloma virus in laryngeal squamous cell carcinoma].
Using immunohistochemical techniques with p53 monoclonal antibody DO-7 and polymerase chain reaction with type specific primers, we detected the expression of p53 of laryngeal and hypopharyngeal squamous cell carcinoma (L-HSCC) in 42 patients, tissues around tumor in 25 patients, human papilloma virus (HPV) 16/18 DNA in paraffinembedded carcinoma tissues from 13 patients with laryngeal squamous cell carcinoma (LSCC). The results showed that overexpression of p53 was detected in 54.8% (23/42) of L-GSCC and 20% (5/25) of hyperlasia epithelia, respectively. There was no correlation of p53 overexpression with clinical stages and histological grading of tumors (P > 0.05). HPV16 DNA encoding E6 protein was detected in 23.1% (3/13) LSCC tissues by PCR. The results suggest that overexpression of p53 and HPV infection are not only associated with pathogenesis of this kind of cancer but also cooperated during carcinogenesis.
Xie
M
M
Department of Otolaryngology, Xiangya Hospital, Hunan Medical University, Changsha.
Xiao
J
J
Tao
Z
Z
Luo
J
J
D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000821:virology*; D005260:Female; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000032:analysis; D027383:Papillomaviridae; D030361:Papillomavirus Infections; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*; D014412:Tumor Virus Infections
C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins; D016159:Tumor Suppressor Protein p53