A 30s PAS stain for frozen section analysis of surgical margins of vulvectomy in Paget's disease.
Application of a modified periodic-acid Schiff's procedure (PAS) technique in frozen section analysis of surgical margins of vulvectomy specimen in Paget's disease is described. The reaction obtained using a microwave procedure takes only 30 seconds. This method was found to be useful in identifying Paget cells on the margins thought to be free of disease by conventional histological evaluation.
Fishman
A
A
Gynecologic-Oncology Unit, Meir Hospital - Sapir Medical Center, Kfar-Saba, Israel.
Lew
S
S
Altaras
M
M
Beyth
Y
Y
Bernheim
J
J
D000368:Aged; D005260:Female; D005629:Frozen Sections; D006801:Humans; D010145:Paget Disease, Extramammary / Q000473:pathology* / Q000601:surgery*; D010503:Periodic Acid-Schiff Reaction; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D014844:Vulva / Q000473:pathology / Q000601:surgery; D014846:Vulvar Neoplasms / Q000473:pathology* / Q000601:surgery*