title: In vivo left ventricular function and collagen expression in aldosterone/salt-induced hypertension.
abstract: Cardiac fibrosis is linked to aldosterone-induced hypertension, but the effects on in vivo left ventricular (LV) function are not established. We studied the relations between in vivo LV function and aldosterone/salt cardiac fibrosis. Adult guinea pigs (GPs) were treated for 3 months with an aldosterone infusion and high-salt diet. This treatment induced arterial hypertension (+35%) and moderate LV hypertrophy (LVH; +60%) without right ventricular (RV) hypertrophy. Echo-Doppler LV assessment demonstrated unaltered cardiac output, stroke volume, or LV relaxation. Type I collagen messenger RNA (mRNA) was significantly increased in both ventricles (LV, +48%; RV, +77%) and accompanied by a significant increase in total collagen deposition (LV, from 0.52% in controls to 4.4% in treated GPs; RV, from 0.82 to 5.5% in treated GPs). Plasma norepinephrine levels increased 2.6-fold (p < 0.01) and correlated with the increase in collagen deposition in both ventricles. Collagen content was not correlated with hypertension or LVH. We conclude that aldosterone administration induces cardiac collagen accumulation and a sympathetic stimulation, which might preserve systolic and diastolic function.
authors.affiliation: Laboratoire de Pharmacologie Cardiovasculaire, Institut Federatif de Recherche de Génetique et Physiopathologie Cardiovasculaire INSERM-Hôpital Pitié-Salpêtrière, Hôpital Pitié-Salpêtrière, Paris, France.
authors.forename: J F
authors.initials: JF
authors.lastname: Ramirez-Gil
authors.forename: C
authors.initials: C
authors.lastname: Delcayre
authors.forename: V
authors.initials: V
authors.lastname: Robert
authors.forename: M
authors.initials: M
authors.lastname: Wassef
authors.forename: P
authors.initials: P
authors.lastname: Trouve
authors.forename: N
authors.initials: N
authors.lastname: Mougenot
authors.forename: D
authors.initials: D
authors.lastname: Charlemagne
authors.forename: P
authors.initials: P
authors.lastname: Lechat
mesh_terms: D000450:Aldosterone; D000818:Animals; D003094:Collagen / Q000096:biosynthesis* / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism; D005355:Fibrosis / Q000209:etiology; D006168:Guinea Pigs; D006728:Hormones / Q000097:blood; D006973:Hypertension / Q000139:chemically induced / Q000150:complications / Q000378:metabolism / Q000503:physiopathology*; D017379:Hypertrophy, Left Ventricular; D017380:Hypertrophy, Right Ventricular; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012492:Salts; D016277:Ventricular Function, Left
chemical_list: D004573:Electrolytes; D006728:Hormones; D012333:RNA, Messenger; D012492:Salts; D000450:Aldosterone; D003094:Collagen