Liquid chromatographic separation of some PTH-amino acids.
Liquid chromatographic studies on the separation of ten PTH-amino acids were carried out using normal phase untreated silica gel plates, C-18 RP precoated plates and RP-HPLC. Resolution of a complex mixture of PTH-amino acids was achieved using all the three types. Certain new successful solvent systems have been worked out in each case. HPLC was carried out with Lichrosphere 100 RP-18 (5 microns) column. Acetonitrile and sodium acetate buffer of pH 4.0 was used for reversed phase chromatography while for normal phase TLC combinations of chloroform-acetonitrile and chloroform-tetrahydrofuran were applied.
Bhushan
R
R
Department of Chemistry, University of Roorkee, India.
Agarwal
R
R
D000596:Amino Acids / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D007202:Indicators and Reagents; D010669:Phenylthiohydantoin