title: Neutrophilia of infection/inflammation: are we really dealing with "inflamed" leukocytes?
abstract: We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and CD62L antigens to study the relationship between their expression and leukocytosis in patients with infection/inflammation, acute stress and healthy volunteers. Mean +/- S.D. channel fluorescence intensity of CD11b/CD18 antigen on peripheral blood polymorphonuclears did not differ between patients with infection/ inflammation (173+/-78) and controls (167+/-72), but was significantly (p = 0.04) reduced in stress (135+/-60). No correlation was found between CD11b/CD18 antigen level and either polymorphonuclears absolute number or serum C-reactive protein. A significant negative correlation was noted between CD62L antigen expression on polymorphonuclears and their absolute number. We assume that cells with increased CD11b/CD18 surface concentrations are retained in the capillaries and that part of the leukocytes in the peripheral blood are stressed leukocytes with reduced CD11b/CD18. Thus, leukocytes detected in peripheral blood are not necessarily the most "inflamed" ones.
authors.affiliation: Department of Internal Medicine D, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
authors.forename: Y
authors.initials: Y
authors.lastname: Sasson
authors.forename: D
authors.initials: D
authors.lastname: Zeltser
authors.forename: O
authors.initials: O
authors.lastname: Rogowski
authors.forename: M
authors.initials: M
authors.lastname: Kassirer
authors.forename: N
authors.initials: N
authors.lastname: Maharshak
authors.forename: A
authors.initials: A
authors.lastname: Nissenkorn
authors.forename: E
authors.initials: E
authors.lastname: Leibovitz
authors.forename: P
authors.initials: P
authors.lastname: Halperin
authors.forename: D
authors.initials: D
authors.lastname: Sharvit
authors.forename: P
authors.initials: P
authors.lastname: Sorkine
authors.forename: N
authors.initials: N
authors.lastname: Arber
authors.forename: S
authors.initials: S
authors.lastname: Berliner
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018821:CD18 Antigens / Q000276:immunology; D003141:Communicable Diseases / Q000097:blood* / Q000276:immunology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007249:Inflammation / Q000097:blood* / Q000276:immunology*; D019041:L-Selectin / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology*
chemical_list: D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D019041:L-Selectin