Cloning of a novel rat gene, DB83, that encodes a putative membrane protein.
Using a partial cDNA sequence and a 5'-RACE technique, we isolated a novel cDNA from rat liver referred to as DB83. DB83 had four hydrophobic trans-membrane domains and one N-myristoylation site as well as multiple possible phosphorylation sites. The db83 gene was highly expressed in the liver and significantly in brain, lungs and kidneys. We suggest that DB83 is a tissue-specific putative membrane protein. p6
Nakadai
T
T
Department of Biology, Faculty of Science, Chiba University, Japan.
Kishimoto
T
T
Kokura
K
K
Ohkawa
N
N
Makino
Y
Y
Muramatsu
M
M
Tamura
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D008099:Liver / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; C118312:Tmem33 protein, rat