Torsional state of DNA in a transcriptionally hyperactive Balbiani ring of polytene chromosomes.
The torsional tension of unconstrained double-helical DNA was determined in transcriptionally hyperactive Balbiani ring 2 (BR2) and in inactive polytene chromosome bands of Chironomus tentans. The method used is based on the dual ability of small intercalating ligands to (a) sense, by differential binding, twists that deviate from that of regular B-form DNA and (b) create positive torsional tension in closed double-stranded DNA, thereby compensating for any negative torsional tension that existed before intercalation. Isolated nuclei of salivary glands were stained with the intercalating fluorescent dye ethidium bromide (EtBr) at various concentrations, and the temporal fluorescence intensity changes (deltaI/I per min) occurring in BR2 and in inactive bands were monitored under a confocal laser scanning microscope during the process of DNA nicking by laser irradiation or DNAase I. From the EtBr concentration at which deltaI/I per min was neither positive nor negative after nicking (i.e. at the equivalence point), the relative twist difference (RTD) was calculated. In bands, it was found to be very small, suggesting that their unconstrained DNA is under low torsional stress. In contrast, the RTD of DNA in highly expanded areas of BR2 was estimated to be negative and of a significant magnitude in absolute terms. This indicates that transcriptionally hyperactive DNA is under considerable negative torsional tension.
Gruzdev
A D
AD
Institute for Cytology and Genetics, Russian Academy of Sciences, Siberian Department, Novosibirsk.
Lezzi
M
M
D000818:Animals; D002683:Chironomidae / Q000235:genetics*; D002871:Chromosome Banding; D004278:DNA, Superhelical / Q000737:chemistry* / Q000378:metabolism; D004996:Ethidium; D018613:Microscopy, Confocal; D009690:Nucleic Acid Conformation; D012260:Ribonucleases / Q000378:metabolism; D012303:Ring Chromosomes; D012469:Salivary Glands / Q000166:cytology; D014158:Transcription, Genetic
D004278:DNA, Superhelical; D012260:Ribonucleases; D004996:Ethidium