Multiple sample PCR amplification and electrophoretic analysis on a microchip.
Polymerase chain reactions (PCRs) were carried out on as many as four DNA samples at a time on a microchip device. The PCR products were then analyzed, either individually or together on the same device, by microchip gel electrophoresis. A standard PCR protocol was used to amplify 199- and 500-base pair (bp) regions of bacteriophage lambda DNA and 346- and 410-bp regions of E. coli genomic and plasmid DNAs, respectively. Thermal lysis of the bacteria was integrated into the PCR cycle. A product sizing medium, poly(dimethylacrylamide), and an intercalating dye for fluorescence detection were used in the electrophoretic analysis of the products. PCR product sizes were determined by coelectrophoresis with marker DNA.
Waters
L C
LC
Oak Ridge National Laboratory, Tennessee 37831-6142, USA.
Jacobson
S C
SC
Kroutchinina
N
N
Khandurina
J
J
Foote
R S
RS
Ramsey
J M
JM
D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004586:Electrophoresis / Q000379:methods*; D005784:Gene Amplification; D016133:Polymerase Chain Reaction / Q000379:methods*
D004269:DNA, Bacterial; D004279:DNA, Viral