[Effects of hypoxia and anisodamine on products of TXA2 and PGI2 in cultured intra-pulmonary arteriolar smooth muscle cells].
The changes of contents of TXB2 and 6-Keto-PGF1a were studied in severely acute hypoxic cultured intra-pulmonary arteriolar smooth muscle cells (PASMCs) under the action of anisodamine. The results demonstrated that the contents of TXB2 and 6-Keto-PGF1a and their ratio were significantly increased in severe acute hypoxic PASMCs' medium. The content of TXB2 decreased significantly, but the content of 6-Keto-PGF1a was hardly affected by anisodamine under normoxia and hypoxia. These findings suggest that acute and severe hypoxia results in pulmonary vascular constriction through increased production of PASMCs and liberation of TXA2, or PGI2, and increased TXA2/PGI2 ratio. The latter effect of hypoxia could be prevented by anisodamine, which antagonized the effect of hypoxia induced pulmonary vasoconstriction.
Hu
D H
DH
Laboratory of Cardiovascular Physiology, Hunan Medical University, Changsha.
Liu
H J
HJ
Ma
C T
CT
D000818:Animals; D001160:Arterioles / Q000166:cytology; D015687:Cell Hypoxia; D002478:Cells, Cultured; D011464:Epoprostenol / Q000096:biosynthesis*; D008168:Lung / Q000098:blood supply*; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D011817:Rabbits; D012991:Solanaceous Alkaloids / Q000494:pharmacology*; D013929:Thromboxane B2 / Q000096:biosynthesis*; D014665:Vasodilator Agents / Q000494:pharmacology
D012991:Solanaceous Alkaloids; D014665:Vasodilator Agents; C003922:anisodamine; D013929:Thromboxane B2; D011464:Epoprostenol