title: Histochemical characteristics of Gyrodactylus derjavini parasitizing the fins of rainbow trout (Oncorhynchus mykiss).
abstract: Histochemical studies of enzyme activities and structural elements in Gyrodactylus derjavini Mikailov, 1975 parasitizing fins of Oncorhynchus mykiss Walbaum were conducted. Marked activities of non-specific esterase, acid phosphatase, alkaline phosphatase and amino-peptidase were found in the intestinal caeca of the parasite. A strong activity of acetylcholinesterase was seen in the nervous system. Extraintestinal non-specific and eserine-sulphate resistant esterase was localized in the distal part of the hamulus sheath. Activities of peroxidase and glucuronidase were not detected. In the embryo, developing hamuli were enclosed in a sheath rich in phospholipids. Deposits of neutral lipids were sparse. The fully developed ventral and dorsal hamulus bars stained strongly for calcium. Lectin binding assays showed a mannose rich region in the cephalic duct openings, strong reactions for galactose in the glycocalyx whereas reactions for lactose were weak. These findings are discussed with respect to the parasite-host relationship.
authors.affiliation: Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark. kurt.buchmann@vetmi.kvl.dk
authors.forename: K
authors.initials: K
authors.lastname: Buchmann
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis; D002589:Cestoda / Q000737:chemistry* / Q000201:enzymology; D004625:Embryo, Nonmammalian / Q000737:chemistry; D004798:Enzymes / Q000032:analysis; D006651:Histocytochemistry; D006790:Host-Parasite Interactions; D007422:Intestines / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008358:Mannose / Q000032:analysis; D009420:Nervous System / Q000737:chemistry; D017686:Oncorhynchus mykiss / Q000469:parasitology*
chemical_list: D004798:Enzymes; D008055:Lipids; D008358:Mannose; D002118:Calcium