[Experimental study in vivo on implantation of autogenous tendon cells after combining culture with carbon fibers].
In order to investigate the possibility of repairing injuried tendon with living artificial tendon, after combining culture, subcultured autogenous tendon cells with carbon fibers were implanted into the calcaneous tendon of rabbits. In different stages, the synthesis of type I collagen and their relevant morphological changes were observed. The results showed as follows: after implantation, tendon cells continued proliferating. Four weeks after implantation, tendon cells were detached from the carbon fibers and proliferated and produced collagen among the carbon fibers. The collagen fibrils were linked with each other to formed a dense structure. In the linkage site, the collagen fibrils originated from the implants joined to that from the ruptured end of the tendon, which meaned that the implant was healed with the recipient tendon. Observed under scanning electronic microscope, the tendon cells were lined among the carbon fibers evenly and in order, the collagen fibrils joined each other and formed an network, the fibrils were lined parallel to the carbon fibers. Under transparent electron microscope, the nucleolus were clear and organelle were abundant.
Zhang
Q
Q
Department of Orthopedic Surgery, 1st University Hospital, West China University of Medical Sciences, Chengdu, Sichuan, P. R. China.
Yang
Z
Z
Peng
W
W
D000818:Animals; D002244:Carbon; D000077482:Carbon Fiber; D002478:Cells, Cultured; D003470:Culture Media; D019736:Prostheses and Implants; D011817:Rabbits; D013710:Tendons / Q000166:cytology* / Q000637:transplantation*
D000077482:Carbon Fiber; D003470:Culture Media; D002244:Carbon