title: Intraabdominal adhesion formation after preperitoneal dissection in the murine model.
abstract: BACKGROUND
  The laparoscopic approach to hernia repair has been advocated by many as a potentially superior method of herniorraphy. Several techniques have been described, each with its own proposed advantages. These techniques involve different anatomic approaches, the most recent of which is the totally extraperitoneal approach (TEPA). One presumed advantage of the extraperitoneal approach is the avoidance of adhesion formation because the peritoneum is not entered and mesh is not placed in direct contact with intra-abdominal structures. We hypothesize, however, that when the peritoneum is dissected from the abdominal wall, it is partially devascularized, leading to scar formation and potential adhesion formation. This would suggest that the TEPA method of herniorraphy may not completely avoid the risks of intra-abdominal adhesion formation.
  
  
  METHODS
  After appropriate approval was obtained, 88 male Sprague-Dawley rats were divided into two equal groups. One group underwent laparotomy followed by careful blunt dissection of the peritoneum from the left abdominal wall. The control group underwent laparotomy without manipulation of the peritoneum. All animals were re-explored 14 days later, and the abdominal cavity was examined for adhesions. The type and location of any adhesion was recorded.
  
  
  RESULTS
  Adhesion formation occurred in 10 of 44 (23%) subjects in the peritoneal dissection group, compared with 3 of 44 (7%) in the nondissection group (p < 0.05).
  
  
  CONCLUSIONS
  Dissection of the peritoneum from the overlying abdominal wall in the murine model leads to intra-abdominal adhesion formation. This suggests that peritoneal dissection in the TEPA method of herniorraphy may lead to intra-abdominal adhesion formation.
authors.affiliation: Washington Institute of Surgical Endoscopy, 2150 Pennsylvania Avenue, N.W. 6B-412, The George Washington University, Washington, DC 20037, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Halverson
authors.forename: W L
authors.initials: WL
authors.lastname: Barrett
authors.forename: P
authors.initials: P
authors.lastname: Bhanot
authors.forename: J E
authors.initials: JE
authors.lastname: Phillips
authors.forename: A R
authors.initials: AR
authors.lastname: Iglesias
authors.forename: L K
authors.initials: LK
authors.lastname: Jacobs
authors.forename: J M
authors.initials: JM
authors.lastname: Sackier
mesh_terms: D000818:Animals; D016009:Chi-Square Distribution; D004195:Disease Models, Animal; D004210:Dissection; D006552:Hernia, Inguinal / Q000601:surgery*; D010535:Laparoscopy / Q000009:adverse effects* / Q000379:methods; D008297:Male; D010532:Peritoneal Diseases / Q000209:etiology*; D010537:Peritoneum / Q000601:surgery*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D000267:Tissue Adhesions / Q000209:etiology