title: Elisa comparison of three whole-cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Kosice, eastern Slovakia.
abstract: Three antigens of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto--Slovak strain Ir 105, B. garini - Slovak strain K 24 and B. burgdorferi sensu stricto--American strain B 31) were compared by ELISA on a group of dogs from urban agglomeration of Kosice, eastern Slovakia. Of 256 serum samples from dogs examined for the presence of anti-Borrelia IgG antibodies, 128 (50%) were positive with Ir 105 antigen, 107 (41.7%) with K 24 and 74 (28.9%) with B 31. The seroprevalence between strains B 31 and K 24 and B 31 and Ir 105 differed statistically significantly (test chi2, p < 0.05), however, the difference between strains K 24 and Ir 105 was insignificant. A significantly higher seroprevalence of all the strains examined was detected in hunting dogs (test chi2, p < 0.05) when compared with service and pet dogs. The seroprevalence correlated with the frequency of outing the dogs in woody areas with the occurrence of borreliae in ticks (R = 0.5 or 0.7) as well as with the frequency of finding engorged ticks (R = 0.5). An epizootiological anamnesis showed a fair specificity in all the strains examined. The Slovak strains showed the higher consistency of positive and negative findings (70%) but statistically lower specificity than the American strain.
authors.affiliation: Parasitological Institute of the Slovak Academy of Sciences, kosice, Slovak Republic. astefan@saske.sk
authors.forename: A
authors.initials: A
authors.lastname: Stefançíková
authors.forename: G
authors.initials: G
authors.lastname: Tresová
authors.forename: B
authors.initials: B
authors.lastname: Pet'ko
authors.forename: I
authors.initials: I
authors.lastname: Skardová
authors.forename: E
authors.initials: E
authors.lastname: Sesztàková
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis* / Q000097:blood; D025065:Borrelia burgdorferi / Q000276:immunology*; D004283:Dog Diseases / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary*; D005260:Female; D008193:Lyme Disease / Q000175:diagnosis / Q000662:veterinary*; D008297:Male; D011237:Predictive Value of Tests; D018154:Slovakia
chemical_list: D000942:Antigens, Bacterial