title: Covalent coupling of immunoglobulin G to self-assembled monolayers as a method for immobilizing the interfacial-recognition layer of a surface plasmon resonance immunosensor.
abstract: Protocols have been developed for the random and site-directed covalent coupling of immunoglobulin G [anti-hIgG] [IgG] to silver surfaces modified with a self-assembled monolayer [SAM] of thioctic acid, mercaptopropionic acid [MPA], L-cysteine or 4-aminothiophenol [PATP]. A surface plasmon resonance [SPR] immunosensor fabricated with a more ordered and hydrophilic IgG-SAM-silver interfacial layer, demonstrates an increased ability for performing sensitive and selective assay of human immunoglobulin G [hIgG] compared with a device fabricated with a physically-adsorbed IgG-silver interfacial-layer due to reduced levels of non-specific binding. Detection limits [DL] for hIgG from serum down to 6 micrograms/ml (40 nM) and assay sensitivities up to 0.24 ng hIgG/mm2/nM are reported.
authors.affiliation: Institute of Biotechnology, University of Cambridge, UK. admin@biotech.cam.ac.uk
authors.forename: D M
authors.initials: DM
authors.lastname: Disley
authors.forename: D C
authors.initials: DC
authors.lastname: Cullen
authors.forename: H X
authors.initials: HX
authors.lastname: You
authors.forename: C R
authors.initials: CR
authors.lastname: Lowe
mesh_terms: D015374:Biosensing Techniques; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G / Q000097:blood*; D012680:Sensitivity and Specificity; D012834:Silver
chemical_list: D007074:Immunoglobulin G; D012834:Silver