In vitro culture system for iris-pigmented epithelial cells for molecular analysis of transdifferentiation.
Dissociated cells of the iris-pigmented epithelium (IPE) from a 1-day-old chick grew in monolayer culture and stably maintained their differentiated state when cultured with standard culture medium. After replacement of the control medium by EdFPH medium, which is effective in inducing dedifferentiation of retinal pigmented epithelium (RPE) cells, all cells rapidly lost pigment granules, proliferated intensively, and dedifferentiated. By further addition of ascorbic acid, dedifferentiated cells accumulated and formed a large number of lentoids. This system provides a useful opportunity for analyzing cellular and molecular mechanism involved in each step of transdifferentiation. Furthermore, Northern blot data indicates that the up-regulation of pax-6 gene could be an important event during lens regeneration as well as during normal lens development.
Kosaka
M
M
Department of Developmental Biology, National Institute for Basic Biology, Okazaki, Aichi, 444, Japan. mkosaka@phys2.med.osaka-u.ac.jp
Kodama
R
R
Eguchi
G
G
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis; D015152:Blotting, Northern; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D048429:Cell Size / Q000187:drug effects; D002478:Cells, Cultured; D002645:Chickens; D003459:Crystallins / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005136:Eye Proteins; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D005786:Gene Expression Regulation; D018398:Homeodomain Proteins; D007498:Iris / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology / Q000502:physiology; D008543:Melanins / Q000378:metabolism; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects; D012097:Repressor Proteins; D012160:Retina; D015533:Transcriptional Activation / Q000187:drug effects
D015415:Biomarkers; D003459:Crystallins; D004268:DNA-Binding Proteins; D005136:Eye Proteins; D018398:Homeodomain Proteins; D008543:Melanins; D000071841:PAX6 Transcription Factor; D051761:Paired Box Transcription Factors; D012097:Repressor Proteins; D016222:Fibroblast Growth Factor 2; D001205:Ascorbic Acid