Single amino acid change in DNA polymerase is associated with foscarnet resistance in a varicella-zoster virus strain recovered from a patient with AIDS.
The genetic characterization of a foscarnet-resistant strain of varicella-zoster virus (VZV) that was isolated from a patient with AIDS is reported. Compared with the sequence of the Dumas reference strain, this strain, which was isolated from a patient in whom foscarnet treatment failed, had two point mutations. The emergence of one of the mutations, which includes a change from a glutamic acid to a lysine at position 512 in the DNA polymerase, suggests that this mutation is implicated in the VZV foscarnet resistance. The other mutation, which replaces serine 863 by a glycine, is also present in 2 susceptible strains--Oka and a wild-type isolate.
Visse
B
B
Laboratoire de Virologie, CERVI, Hôpital Pitié-Salpêtrière, Paris, France.
Dumont
B
B
Huraux
J M
JM
Fillet
A M
AM
D017088:AIDS-Related Opportunistic Infections / Q000821:virology; D000328:Adult; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000998:Antiviral Agents / Q000494:pharmacology*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004352:Drug Resistance, Microbial / Q000235:genetics; D017245:Foscarnet / Q000494:pharmacology*; D006562:Herpes Zoster / Q000150:complications / Q000821:virology; D014645:Herpesvirus 3, Human / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D000998:Antiviral Agents; D017931:DNA Primers; D017245:Foscarnet; D004259:DNA-Directed DNA Polymerase