Synthesis of 14 C-radiolabeled oligonucleotides with a novel phosphoramidite reagent.
A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-14C]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus.
Guzaev
A
A
Department of Medicinal Chemistry, Isis Pharmaceuticals, Carlsbad, CA 92008.
Boyode
B
B
Balow
G
G
Tivel
K L
KL
Manoharan
M
M
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds; D013936:Thymidine / Q000031:analogs & derivatives*
D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C028273:5'-O-dimethyltritylthymidine; D013936:Thymidine