Brief exposure of mice to 60 Hz magnetic fields reduces the analgesic effects of the neuroactive steroid, 3alpha-hydroxy-4-pregnen-20-one.
Relatively weak, extremely low frequency (ELF), magnetic fields have been shown to exert a variety of biological effects, although the modes of action remain to be established. Neuroactive steroids and neurosteroids have been shown to produce a diverse range of rapid centrally mediated behavioral and physiological effects that are reported to be sensitive to magnetic fields. Here we show that brief exposure of male mice to an ELF magnetic field (30 min, 60 Hz, 141 microT peak) significantly reduces the analgesic effects arising from intracerebroventricular (i.c.v.) administration of the centrally produced allylic neuroactive steroid, 3alpha-hydroxy-4-pregnen-20-one (3alphaHP) and that the dihydropyridine (DHP) calcium channel antagonists, diltiazem and nifedipine, block the inhibitory effects of the 60 Hz ELF on 3alphaHP-induced analgesia. These results indicate that exposure to 60 Hz ELF affects the analgesic effects of neuroactive steroids such as 3alphaHP through alterations in calcium channel function. These findings raise the possibility that ELF magnetic fields may, in part, exert their actions through effects on diverse neuroactive steroid modulated processes.
Kavaliers
M
M
Department of Psychology, Social Sciences Centre, University of Western Ontario, London, Canada. kavalier@julian.uwo.ca
Wiebe
J P
JP
Ossenkopp
K P
KP
D004092:20-alpha-Dihydroprogesterone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000528:radiation effects; D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology / Q000528:radiation effects*; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000528:radiation effects; D004574:Electromagnetic Fields; D007276:Injections, Intraventricular; D008297:Male; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000528:radiation effects
D000700:Analgesics; D004092:20-alpha-Dihydroprogesterone; C052610:3-hydroxypregn-4-en-20-one