Tumour necrosis factor microsatellites in reactive arthritis.
The purpose was to study tumour necrosis factor (TNF)-a, -b and -c microsatellites as potential new susceptibility markers for reactive arthritis (ReA). Fifty-nine patients typed for HLA-B27 were studied for frequencies of TNF microsatellite alleles and compared with allele frequencies determined from 285 random haplotypes and 46 healthy HLA-B27-positive controls. TNFa, -b and -c microsatellite sequences were amplified by the polymerase chain reaction, and the size of the product was defined by an automated sequencer. The frequencies of TNFa6 and -c1 alleles were found to be increased in patients with ReA, whereas TNFa11 and -c2 frequencies were decreased as compared to control haplotypes. The increase in the c1 allele in patients with ReA independently from HLA-B27 suggests that it might be a new susceptibility marker for the disease. The association of ReA with other alleles was due to a linkage disequilibrium with HLA-B27.
Tuokko
J
J
Turku Immunology Centre and Department of Medical Microbiology, University of Turku, Finland.
Koskinen
S
S
Westman
P
P
Yli-Kerttula
U
U
Toivanen
A
A
Ilonen
J
J
D000483:Alleles; D016918:Arthritis, Reactive / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D004198:Disease Susceptibility; D015796:HLA-B27 Antigen / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D018895:Microsatellite Repeats / Q000235:genetics*; D000091402:Prohibitins; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000235:genetics
D015415:Biomarkers; D015796:HLA-B27 Antigen; C000717331:PHB2 protein, human; D000091402:Prohibitins; D014409:Tumor Necrosis Factor-alpha