title: Matrix-mesangial cell interaction modulates migration of macrophages.
abstract: BACKGROUND
  Macrophages (Møs) have been demonstrated to play an important role in immune-mediated renal injury. Accumulation of macrophages in the mesangium has been reported to be a key event in the development of focal glomerulosclerosis. We hypothesized that mesangial cells (MCs) and matrix interaction may be a determinant for the migration of Møs into the mesangium. Therefore, we studied the effect of the interaction between matrix and MCs on the migration of Møs.
  
  
  METHODS
  Mouse MCs were plated on Petri dishes coated either with buffer, collagen type I, III, IV, or Matrigel in media containing 1% fetal calf serum for 48 hours. Subsequently, supernatants were collected and stored. The effect of these supernatants (conditioned media) was evaluated on the migration of Møs across a filter in a modified Boyden chamber.
  
  
  RESULTS
  Conditioned media from MCs grown on Matrigel (MC-Matrigel interaction products, MC-MGP) enhanced the migration of macrophages across a filter in a modified Boyden chamber when compared with conditioned media from MCs grown on plastic, collagen type I, type III, or type IV (MC-PP, MC-CI, MC-CIII, and MC-CIV). MC-MGP enhanced the migration of Møs in a dose dependent manner. Anti-MCP-1 antibodies attenuated (P < 0.05) the MC-MGP-induced Mø migration (MC-MGP, 16.8 +/- 2.5 vs MC-MGP + anti-MCP-1 antibody, 6.5 +/- 1.2 migrated macrophages/field, n = 12). Anti-TGF-beta antibodies did not attenuate MC-MGP-induced Mø migration. MCs grown on Matrigel showed a 5-fold increase of MCP-1 mRNA when compared with cells grown on plastic or collagen type IV.
  
  
  CONCLUSIONS
  The present study suggests that matrix components may modulate the migration of Møs. This effect of MC-matrix interaction on macrophage migration may be mediated through the generation of MCP-1.
authors.affiliation: Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, NY 11040, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Singhal
authors.forename: N
authors.initials: N
authors.lastname: Franki
authors.forename: N
authors.initials: N
authors.lastname: Gibbons
authors.forename: K
authors.initials: K
authors.lastname: Reddy
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D002450:Cell Communication / Q000502:physiology*; D002452:Cell Count; D002460:Cell Line; D002464:Cell Migration Inhibition; D002465:Cell Movement / Q000502:physiology*; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology; D003094:Collagen / Q000494:pharmacology; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004338:Drug Combinations; D005109:Extracellular Matrix / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D005920:Glomerular Mesangium / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D007797:Laminin / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000502:physiology*; D051379:Mice; D011509:Proteoglycans / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000276:immunology
chemical_list: D001672:Biocompatible Materials; D018932:Chemokine CCL2; D017077:Culture Media, Conditioned; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; C059485:matrigel; D003094:Collagen