[The expression of IGF-1 and its receptor in cultured tendon cell].
In order to study the expression change of insulin-like growth factor-1 (IGF-1) and its receptor genes in different generations of tendon cell in culture, Dig-labeled synthesized oligonucleotide probes were used to detect the mRNA expression in primary, 6th and 13th generation of tendon cell. The results showed that IGF-1 receptor mRNA was expressed in all of the 3 above generation tendon cells. IGF-1 mRNA was expressed only in primary and 6th generation cells. Tendon cell of 13th generation did not express IGF-1 mRNA. It might suggest that the absence of IGF-1 mRNA expression be one of the causes which led to the decrease of reproductive ability of 13th generation tendon cell.
Xiang
Z
Z
Department of Orthopedic Surgery, First University Hospital, West China University of Medical Sciences, Chengdu, P. R. China.
Yang
Z
Z
Xia
Q
Q
D002478:Cells, Cultured; D016922:Cellular Senescence; D005333:Fetus; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis; D017526:Receptor, IGF Type 1 / Q000096:biosynthesis*; D013710:Tendons / Q000166:cytology / Q000378:metabolism*
D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I; D017526:Receptor, IGF Type 1