The contribution of nitric oxide to renal vascular wall thickening in rats with L-NAME-induced hypertension.
We investigated the mechanisms of renal vascular wall thickening in a rat model of N-nitro L-arginine methyl ester (L-NAME)-induced hypertension. To separate the effects of L-NAME-induced hypertension from other effects of nitric oxide (NO) inhibition, we created two models of L-NAME-induced hypertension: both had the same blood pressure level but NO inhibition was moderate in one group (group M) and severe in the other (group S). Urinary excretion of nitrates and nitrites was lower in group S than in group M. Wall thickening and lipid deposition in renal vessels were significantly greater in group S than in groups M. Simple and multiple regression analyses indicated that renal vascular wall thickening was more strongly correlated with lipid deposition than with blood pressure. The number of vessels positive for staining with Sudan black B was negatively correlated with urinary NO excretion. Expression of fibronectin and transforming growth factor-beta was greater in the Sudan black B-positive than in the Sudan black B-negative vessels, suggesting that extracellular matrix production was increased in vessels with lipid deposition. Lipid deposition and increased production of extracellular matrix may contribute to renal vascular wall thickening in L-NAME-induced hypertension. Some mechanisms independent of hypertension play important roles in vascular wall thickening induced by NO inhibition.
Yoneyama
T
T
Department of Clinical Nutrition, School of Food and Nutritional Sciences, University of Shizuoka, Japan. yoneyama@fns1.u-shizuoka-ken.ac.jp
Ohkawa
S
S
Watanabe
T
T
Odamaki
M
M
Kumagai
H
H
Kimura
M
M
Hishida
A
A
D000199:Actins / Q000378:metabolism; D000818:Animals; D001055:Apolipoproteins B / Q000378:metabolism; D001158:Arteries / Q000378:metabolism / Q000473:pathology*; D001794:Blood Pressure / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D005353:Fibronectins / Q000378:metabolism; D006973:Hypertension / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology* / Q000652:urine; D007668:Kidney / Q000098:blood supply*; D008297:Male; D008856:Microscopy, Fluorescence; D016253:Microscopy, Immunoelectron; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology*; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000502:physiology*; D017672:Nitrogen Compounds / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D012044:Regression Analysis
D000199:Actins; D001055:Apolipoproteins B; D005353:Fibronectins; D017672:Nitrogen Compounds; D009569:Nitric Oxide; D002784:Cholesterol; D019331:NG-Nitroarginine Methyl Ester