Determination of common drugs of abuse in body fluids using one isolation procedure and liquid chromatography--atmospheric-pressure chemical-ionization mass spectromery.
A method for determining opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol, and ibogaine), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester) and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented. Aliquots (0.5-1.5 mL) of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid-phase extraction method (C18 cartridges). The extracts were subjected to liquid chromatographic-atmospheric-pressure chemical-ionization mass spectrometric examination using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries were high for almost all drugs and the extracts were very clean. The procedure was applied for routine forensic casework.
Bogusz
M J
MJ
Institute of Forensic Medicine, Aachen University of Technology, Germany.
Maier
R D
RD
Krüger
K D
KD
Kohls
U
U
D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D013287:Illicit Drugs / Q000032:analysis*; D007118:Immunoassay; D066298:In Vitro Techniques; D008238:Lysergic Acid Diethylamide / Q000032:analysis / Q000097:blood / Q000652:urine; D009294:Narcotics / Q000032:analysis / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*
D013287:Illicit Drugs; D009294:Narcotics; D008238:Lysergic Acid Diethylamide; D003042:Cocaine