Study on a fluorescence-enhanced system of 1,2,4-trihydroxyanthroquinone-Be(2+)-DNA.
Fluorescence-enhancement of 1,2,4-trihydroxyanthroquinone (THAQ)-Be2+ complex enhanced by nucleic acid was studied. Experimental results revealed that double-stranded DNA can enhance remarkably the fluorescence intensity of THAQ-Be2+, while RNA cannot. Based on these results, a fluorescence method for the selective determination of DNA in the presence of RNA was developed. Maximum fluorescence intensity was found in the pH range 3.6-4.5 with maximum excitation and emission wavelengths at 510 and 565 nm, respectively. Under optimum conditions, the calibration graph was linear over the range 0.08-18 micrograms ml-1 for double-stranded fish sperm DNA (fsDNA) with the detection limit being 2.75 x 10(-8) g ml-1. The method was applied for the determination of DNA in synthetic samples. The relative S.D. for five replicates was within 4%. In addition, the interaction mechanism of THAQ-Be2+ with DNA was also discussed.
Yang
J
J
Department of Chemistry, Shandong University, Jinan, People's Republic of China.
Lin
C
C
Zhang
G
G
Gao
Z
Z
Wu
X
X
Huang
X
X
D000880:Anthraquinones / Q000737:chemistry* / Q000378:metabolism; D001608:Beryllium; D002021:Buffers; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012313:RNA; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
C116389:1,2,4-trihydroxyanthraquinone Be; D000880:Anthraquinones; D002021:Buffers; D012997:Solvents; D012313:RNA; D004247:DNA; D001608:Beryllium