title: T cells mediate treatment of six-day-old cytokine-gene-transfected mouse mammary tumor.
abstract: We have previously shown that immunologically different mouse mammary cancer cell lines induce antitumor responses after IL2 or IL4 gene transfection. We now report the ability of cytokine-transfected tumors to induce eradication of established wild-type tumor. Animals with 6-day-old tumor treated with IL2-transfected cells also had significantly smaller tumors 2.8 and 1.7 cm2 (EMT6 and 410.4). Findings were similar for IL4-transfected cells. Tumor infiltrating lymphocytes or cells from draining lymph nodes demonstrated tumor-specific in vitro cytotoxicity. Immunohistochemical studies revealed T cell infiltrates in transfected tumors.
authors.affiliation: Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass. 02115, USA. YMatory@bics.bwh.harvard.edu
authors.forename: Y L
authors.initials: YL
authors.lastname: Matory
authors.forename: D M
authors.initials: DM
authors.lastname: Dorfman
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: R A
authors.initials: RA
authors.lastname: Spanjaard
authors.forename: P S
authors.initials: PS
authors.lastname: Goedegebuure
authors.forename: T J
authors.initials: TJ
authors.lastname: Eberlein
mesh_terms: D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
chemical_list: D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D015847:Interleukin-4