Fission yeast Csk1 is a CAK-activating kinase (CAKAK).
Cell cycle progression is dependent on the sequential activity of cyclin-dependent kinases (CDKs). For full activity, CDKs require an activating phosphorylation of a conserved residue (corresponding to Thr160 in human CDK2) carried out by the CDK-activating kinase (CAK). Two distinct CAK kinases have been described: in budding yeast Saccharomyces cerevisiae, the Cak1/Civ1 kinase is responsible for CAK activity. In several other species including human, Xenopus, Drosophila and fission yeast Schizosaccharomyces pombe, CAK has been identified as a complex homologous to CDK7-cyclin H (Mcs6-Mcs2 in fission yeast). Here we identify the fission yeast Csk1 kinase as an in vivo activating kinase of the Mcs6-Mcs2 CAK defining Csk1 as a CAK-activating kinase (CAKAK).
Hermand
D
D
Haartman Institute and Biocentrum Helsinki, University of Helsinki, Haartmaninkatu 3, 00014 Helsinki, Finland.
Pihlak
A
A
Westerling
T
T
Damagnez
V
V
Vandenhaute
J
J
Cottarel
G
G
Mäkelä
T P
TP
D000595:Amino Acid Sequence; D002453:Cell Cycle; D017124:Conserved Sequence; D056748:Cyclin H; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins; D004789:Enzyme Activation; D018628:Gene Dosage; D016153:Genes, Suppressor; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012568:Schizosaccharomyces / Q000201:enzymology*; D029702:Schizosaccharomyces pombe Proteins; D000095043:Cyclin-Dependent Kinase-Activating Kinase
C534751:CCNH protein, human; C534754:CycH protein, Drosophila; D056748:Cyclin H; D016213:Cyclins; D029721:Drosophila Proteins; D029702:Schizosaccharomyces pombe Proteins; D011494:Protein Kinases; C117097:Csk1 protein, S pombe; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D000095043:Cyclin-Dependent Kinase-Activating Kinase