title: Activation of the osteopontin promoter by the orphan nuclear receptor estrogen receptor related alpha.
abstract: Estrogen receptor related alpha (ERRalpha) is an orphan nuclear receptor that is closely related to the estrogen receptor. It is expressed in a variety of adult and embryonic tissues (in particular, at the onset of ossification), as well as in several osteoblastic cell lines. ERRalpha acts as a site-specific, cell-specific transcriptional activator. Here, we show that ERRalpha transactivates the promoter of the mouse osteopontin (OPN) gene, the product of which is a marker of the late stages of osteoblastic differentiation. This effect is cell specific and is exerted through derivatives of the ERRalpha response element. Overexpression of ERRalpha in three different osteoblast-like cell lines results in an elevation of the amount of OPN-corresponding mRNA. Therefore, OPN is a target gene for ERRalpha, pointing to the role of the latter in osteoblast differentiation.
authors.affiliation: Centre National de la Recherche Scientifique UMR 49, Ecole Normale Supérieure de Lyon, France. Jean-Marc.Vanacker@ens-lyon.fr
authors.forename: J M
authors.initials: JM
authors.lastname: Vanacker
authors.forename: C
authors.initials: C
authors.lastname: Delmarre
authors.forename: X
authors.initials: X
authors.lastname: Guo
authors.forename: V
authors.initials: V
authors.lastname: Laudet
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D051717:Fushi Tarazu Transcription Factors; D005786:Gene Expression Regulation / Q000235:genetics; D018398:Homeodomain Proteins; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism*; D053495:Osteopontin; D017354:Point Mutation / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D011960:Receptors, Estrogen / Q000235:genetics*; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D012795:Sialoglycoproteins / Q000235:genetics*; D054339:Steroidogenic Factor 1; D014157:Transcription Factors / Q000235:genetics; D015533:Transcriptional Activation / Q000235:genetics; D014162:Transfection / Q000235:genetics; D000097983:ERRalpha Estrogen-Related Receptor
chemical_list: D004268:DNA-Binding Proteins; D004958:Estradiol; D051717:Fushi Tarazu Transcription Factors; D018398:Homeodomain Proteins; D053495:Osteopontin; D012333:RNA, Messenger; D018160:Receptors, Cytoplasmic and Nuclear; D011960:Receptors, Estrogen; D012795:Sialoglycoproteins; D054339:Steroidogenic Factor 1; D014157:Transcription Factors; D000097983:ERRalpha Estrogen-Related Receptor; C506896:Spp1 protein, mouse