Hyperthermophilic redox chemistry: a re-evaluation.
The redox chemistry of Pyrococcus furiosus rubredoxin and ferredoxin has been studied as a function of temperature in direct voltammetry and in EPR monitored bulk titrations. The Ems of both proteins, measured with direct voltammetry, have a normal (linear) temperature dependence and show no pH dependence. EPR monitoring is not a reliable method to determine the temperature dependence of the Em: upon rapid freezing the proteins take their conformation corresponding to the freezing point of the solution.
Hagedoorn
P L
PL
Wageningen University and Research Centre, Department of Biomolecular Sciences, The Netherlands. peter.leonhagedoorn@epr.bc.wau.nl
Driessen
M C
MC
van den Bosch
M
M
Landa
I
I
Hagen
W R
WR
D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D020027:Pyrococcus furiosus / Q000737:chemistry* / Q000378:metabolism; D012416:Rubredoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature; D017186:Titrimetry
D005288:Ferredoxins; D012416:Rubredoxins