title: Additive effects of the mutations in the beta3-adrenergic receptor and uncoupling protein-1 genes on weight loss and weight maintenance in Finnish women.
abstract: This study examined whether the Trp64Arg mutation in the beta3-adrenergic receptor (beta3AR) and the A-->G mutation in the uncoupling protein-1 (UCP-1) genes have associations with weight loss and subsequent weight maintenance. Seventy-seven obese (body mass index range, 29-46 kg/m2), clinically healthy, premenopausal women were studied. A 12-wk weight reduction by very low calorie diet (VLCD) was followed by a 40-wk weight maintenance phase. The subjects were divided into four groups according to their beta3AR and UCP-1 genotype: no mutation (control; n=37), only Trp64Arg mutation in the beta3AR gene (n=12), only A-->G mutation in the UCP-1 gene (n=23), and both mutations (n=5). Subjects with both mutations had a lower weight reduction during VLCD than the controls [-10.5+/-0.6 (+/-SEM) vs. -14.0+/-0.5 kg; P=0.051, by ANOVA]. During the maintenance phase, weight in subjects with both mutations increased by 5.8+/-1.5 kg, but remained unchanged in the controls (-0.5+/-0.8 kg; P=0.041). The changes in weight in subjects with only one of the mutation were close to the results in the controls. Resting energy expenditure, adjusted for fat mass, fat-free mass, and maximal aerobic power, did not change differently between the groups throughout the study. The results suggest that a combination of the Trp64Arg mutation in the beta3AR and the A-->G mutation in the UCP-1 genes may be associated with faster weight gain after a VLCD.
authors.affiliation: The UKK Institute for Health Promotion and Research, Tampere, Finland. mikael.fogelholm@helsinki.fi
authors.forename: M
authors.initials: M
authors.lastname: Fogelholm
authors.forename: R
authors.initials: R
authors.lastname: Valve
authors.forename: K
authors.initials: K
authors.lastname: Kukkonen-Harjula
authors.forename: A
authors.initials: A
authors.lastname: Nenonen
authors.forename: V
authors.initials: V
authors.lastname: Hakkarainen
authors.forename: M
authors.initials: M
authors.lastname: Laakso
authors.forename: M
authors.initials: M
authors.lastname: Uusitupa
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D001835:Body Weight / Q000502:physiology*; D002352:Carrier Proteins / Q000235:genetics*; D002149:Energy Intake; D004734:Energy Metabolism / Q000502:physiology; D005260:Female; D005387:Finland; D006801:Humans; D007473:Ion Channels; D008565:Membrane Proteins / Q000235:genetics*; D024101:Mitochondrial Proteins; D009154:Mutation / Q000235:genetics / Q000502:physiology*; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D000071256:Uncoupling Protein 1; D015431:Weight Loss / Q000502:physiology
chemical_list: D002352:Carrier Proteins; D007473:Ion Channels; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011943:Receptors, Adrenergic, beta; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1