title: Acute infection of Sindbis virus induces phosphorylation and intracellular translocation of small heat shock protein HSP27 and activation of p38 MAP kinase signaling pathway.
abstract: In general, viral infection is supposed to induce stress responses in the host cell. However, very few detailed observations about virus-induced stress responses have been reported. Here we investigated specific stress responses in Vero cells infected with Sindbis virus (SV), a single-stranded RNA virus, acute infection with which is known to cause apoptotic cell death in the host cells. Prior to the onset of apoptosis, p38 mitogen-activated protein kinase (MAPK) and c-Jun NH2-terminal kinases (JNKs) were activated. Subsequently, a 27-kDa heat shock protein (HSP27) became phosphorylated, and intracellular distribution of HSP27 was changed from the cytoplasm to the perinuclear region. These results indicate that the cellular signaling cascades activated by pro-inflammatory cytokines and environmental stresses are also activated as a result of lytic infection with SV. These responses may contribute to the delayed onset of apoptosis in the host cells and the facilitation of viral replication.
authors.affiliation: Department of Retroviral Regulation, Tokyo Medical and Dental University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Nakatsue
authors.forename: I
authors.initials: I
authors.lastname: Katoh
authors.forename: S
authors.initials: S
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Ikawa
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshinaka
mesh_terms: D000818:Animals; D017209:Apoptosis; D001692:Biological Transport; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation; D006360:Heat-Shock Proteins / Q000378:metabolism*; D007424:Intracellular Fluid / Q000378:metabolism* / Q000821:virology; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D010766:Phosphorylation; D015398:Signal Transduction; D012845:Sindbis Virus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases
chemical_list: D006360:Heat-Shock Proteins; D003609:Dactinomycin; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D048031:JNK Mitogen-Activated Protein Kinases; D020928:Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases