Antigenic and nucleotide characterization of a herpesvirus isolated from Pacific harbor seals (Phoca vitulina richardsii).
This report describes the antigenic and nucleotide characterization of a herpes-like virus that has been isolated from the adrenal tissues of neonatal Pacific harbor seals. Infection with this virus has been previously implicated as a major cause of death of animals undergoing rehabilitation. Comparison and phylogenetic analysis of sequenced fragments of the DNA polymerase, glycoprotein B and glycoprotein D genes, and immunofluorescence assay using herpesvirus-specific monoclonal antibodies, demonstrated close similarity of the Pacific harbor seal herpesvirus to European isolates of phocid herpesvirus-1 (PHV-1) and other alpha-herpesviruses affecting terrestrial carnivores.
King
D P
DP
Marine Mammal Center, Sausalito, California, USA.
Parselles
R
R
Gulland
F M
FM
Lapointe
J M
JM
Lowenstine
L J
LJ
Ferrick
D A
DA
Stott
J L
JL
D000311:Adrenal Glands / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D046048:Seals, Earless / Q000821:virology*
D000956:Antigens, Viral