Inter-strain comparison by pyrolysis mass spectrometry of Staphylococcus aureus isolates associated with nosocomial infection.
Pyrolysis mass spectrometry was used to characterise Staphylococcus aureus isolates from an outbreak of postoperative wound infections on a mixed surgical ward. The PyMS results were compared with those of phage typing. Both suggested a single strain of S. aureus, of phage type 3C, 55, 71, was responsible for six of the 13 wound infections. PyMS differentiated an isolate from a member of staff of similar phage type to the epidemic strain, which had previously been considered to be the point source for the outbreak. PyMS is a rapid and inexpensive technique for investigating nosocomial outbreaks, including those caused by S. aureus and, in this instance, was more discriminatory than phage typing.
Sisson
P R
PR
Public Health Laboratory, General Hospital, Newcastle upon Tyne, UK.
Nagy
E
E
Kòczián
Z
Z
Veszelovsky
E
E
Freeman
R
R
D015373:Bacterial Typing Techniques; D001434:Bacteriophage Typing; D003428:Cross Infection / Q000382:microbiology*; D004196:Disease Outbreaks; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000145:classification* / Q000302:isolation & purification*; D013530:Surgical Wound Infection / Q000382:microbiology*