The survival of adenovirus in multidose bottles of topical fluorescein.
OBJECTIVE
To determine if common ocular adenovirus serotypes survive in vitro in multidose bottles of topical fluorescein (Fluress; Pilkington Barnes Hind, Inc, Sunnyvale, California).


METHODS
Clinical isolates of adenovirus types 8 and 19 were inoculated separately into 10 bottles each of Fluress and maintained at room temperature (25 C). All bottles were titered for adenovirus on A549 cell monolayers at 0, 7, 14, 21, 28, and 49 days.


RESULTS
Adenovirus was recovered from Fluress for up to 21 days for adenovirus type 19 and 28 days for adenovirus type 8.


CONCLUSIONS
A multidose bottle of Fluress contaminated with adenovirus can be a potential source of adenoviral transmission in an ophthalmic office setting.
Kowalski
R P
RP
Department of Ophthalmology, University of Pittsburgh School of Medicine, Pennsylvania, USA. Rege@vision.eei.upmc.edu
Romanowski
E G
EG
Waikhom
B
B
Gordon
Y J
YJ
D000258:Adenovirus Infections, Human / Q000635:transmission / Q000821:virology; D000260:Adenoviruses, Human / Q000502:physiology*; D000287:Administration, Topical; D002724:Chlorobutanol; D004338:Drug Combinations; D004340:Drug Contamination; D004492:Edetic Acid; D015828:Eye Infections, Viral / Q000635:transmission / Q000821:virology; D005452:Fluoresceins; D006801:Humans; D009883:Ophthalmic Solutions; D011205:Povidone; D011343:Procaine / Q000031:analogs & derivatives*; D014776:Virus Cultivation; D014779:Virus Replication
D004338:Drug Combinations; D005452:Fluoresceins; D009883:Ophthalmic Solutions; C010961:Fluress; D011343:Procaine; D004492:Edetic Acid; D011205:Povidone; D002724:Chlorobutanol