Sensitive and specific liquid chromatographic-tandem mass spectrometric assay for barnidipine in human plasma.
A sensitive and specific LC-MS-MS assay has been developed and validated for barnidipine (1-benzyl-3-pyrrolidinyl)methyl-2,6-dimethyl-4(m-nitrophenyl)-1,4-dihydr opyridine-3,5-dicarboxylate). The assay involves a simple and rapid solid-phase extraction procedure. Sample analysis was on a Spherisorb S3ODS2 100 mmX2 mm I.D. column, with a Finnigan TSQ 7000 mass spectrometer, using an electrospray interface and selective reaction monitoring (SRM). The intra- and inter-day precision and accuracy, determined as the coefficient of variation and relative error, respectively, were 11.8% or less. The limit of quantitation was 0.03 ng/ml, and the calibration was linear between 0.03 and 3.0 ng/ml. The method has been used successfully for the measurement of over two thousand human plasma samples from pharmacokinetic clinical trials.
Pawula
M
M
Huntingdon Life Sciences Ltd., Cambridgeshire, UK.
Watson
D
D
Teramura
T
T
Watanabe
T
T
Higuchi
S
S
Cheng
K N
KN
D002121:Calcium Channel Blockers / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D009543:Nifedipine / Q000031:analogs & derivatives* / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002121:Calcium Channel Blockers; C050699:mepirodipine; D009543:Nifedipine