Anti-HIV-1 and anti-HIV-1-protease substances from Ganoderma lucidum.
A new highly oxygenated triterpene named ganoderic acid alpha has been isolated from a methanol extract of the fruiting bodies of Ganoderma lucidum together with twelve known compounds. The structures of the isolated compounds were determined by spectroscopic means including 2D-NMR. Ganoderiol F and ganodermanontriol were found to be active as anti-HIV-1 agents with an inhibitory concentration of 7.8 micrograms ml-1 for both, and ganoderic acid B, ganoderiol B, ganoderic acid C1, 3 beta-5 alpha-dihydroxy-6 beta-methoxyergosta-7,22-diene, ganoderic acid alpha, ganoderic acid H and ganoderiol A were moderately active inhibitors against HIV-1 PR with a 50% inhibitory concentration of 0.17-0.23 mM.
el-Mekkawy
S
S
Research Institute for Traditional Sino-Japanese Medicines, Toyama Medical and Pharmaceutical University, Japan.
Meselhy
M R
MR
Nakamura
N
N
Tezuka
Y
Y
Hattori
M
M
Kakiuchi
N
N
Shimotohno
K
K
Kawahata
T
T
Otake
T
T
D019380:Anti-HIV Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017320:HIV Protease Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D015497:HIV-1 / Q000187:drug effects / Q000472:pathogenicity; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D010946:Plants, Medicinal / Q000737:chemistry*; D018894:Reverse Transcriptase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D019380:Anti-HIV Agents; D004365:Drugs, Chinese Herbal; D017320:HIV Protease Inhibitors; D018894:Reverse Transcriptase Inhibitors