First synthesis of alternating SATE-phosphotriester/phosphodiester prooligonucleotides on solid support.
Prooligonucleotides with alternating S-Acyl-ThioEthyl (SATE) phosphotriester and phosphodiester linkages or a gap were synthesized by elimination of cyanoethyl group with non-nucleophilic base DBU. Their half-lives in the presence of Pig Liver Esterases (PLE) and in total cell extract were determined.
Tosquellas
G
G
Laboratoire de Chimie Bio-Organique, UMR 5625, Université de Montpellier II, France.
Bologna
J C
JC
Morvan
F
F
Rayner
B
B
Imbach
J L
JL
D000818:Animals; D002457:Cell Extracts; D004950:Esterases / Q000378:metabolism; D006207:Half-Life; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis*; D013552:Swine; D013936:Thymidine / Q000737:chemistry
D002457:Cell Extracts; D009841:Oligonucleotides; D004950:Esterases; D013936:Thymidine