The storage of noradrenaline, neuropeptide Y and chromogranins in and stoichiometric release from large dense cored vesicles of the undifferentiated human neuroblastoma cell line SH-SY5Y.
Sucrose gradient centrifugation combined with electron microscopy revealed that undifferentiated SH-SY5Y cells contain predominantly one population of noradrenaline containing vesicles, i.e. large dense cored vesicles. These vesicles have been purified approximately twenty times using sucrose/D2O gradients. Electron microscopy of sucrose/D2O fractions confirms that large dense cored vesicles are enriched in the fractions containing predominantly dopamine- -hydroxylase, chromogranin A, noradrenaline and neuropeptide Y. The membranes of these vesicles contain the typical large dense cored vesicle markers dopamine- -hydroxylase, synaptotagmin, cytochrome b561 and rab 3. Stimulation of SH-SY5Y cells with carbachol and KCl shows that noradrenaline and neuropeptide Y are released in the same proportion as stored in the large dense cored vesicles. The immuno-blot pattern and intensity of chromogranin A and chromogranin B present in large dense cored vesicles and in the released material were definitely the same. This suggests that noradrenaline and the proteins/peptides are released in the same molar stoichiometry as they are stored in large dense cored vesicles. These data provide for the first time experimental evidence that the neuroblastoma cell line SH-SY5Y contains functionally active large dense cored vesicles similar to those of sympathetic neurons and indicate that this cell line is a suitable experimental cell model to study the exocytotic pathway of large dense cored vesicles.
Ou
X M
XM
Laboratory of Neuropharmacology, Department of Medicine, University of Antwerp (UIA), Universiteitsplein 1, B-2610 Wilrijk, Belgium.
Partoens
P M
PM
Wang
J M
JM
Walker
J H
JH
Danks
K
K
Vaughan
P F
PF
De Potter
W P
WP
D002454:Cell Differentiation; D002864:Chromogranins / Q000378:metabolism*; D004705:Endocytosis; D005089:Exocytosis; D006801:Humans; D008954:Models, Biological; D009447:Neuroblastoma; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009478:Neuropeptide Y / Q000378:metabolism*; D009638:Norepinephrine / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D014407:Tumor Cells, Cultured
D002864:Chromogranins; D009478:Neuropeptide Y; D009638:Norepinephrine