A labeled guanidine ligand for studying sweet taste.
A synthesis of a biotinylated-, coumarin-substituted-N,N'-diarylguanidine 1 is reported. This ligand has structural features conducive to studying sweet taste including a fluorescent tag to facilitate spectroscopic studies of binding to protein-receptors for sweet ligands and an anchor for affinity purification.
Feng
Y
Y
Department of Chemistry, Texas A & M University, College Station 77842, USA.
Burgess
K
K
Pledger
D
D
Cairns
N
N
Linthicum
D S
DS
D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000493:pharmacokinetics; D019921:Biotinylation; D002628:Chemoreceptor Cells / Q000502:physiology*; D015195:Drug Design; D005456:Fluorescent Dyes; D006146:Guanidines / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D008024:Ligands; D015394:Molecular Structure; D013050:Spectrometry, Fluorescence; D013549:Sweetening Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000493:pharmacokinetics; D013649:Taste
C116646:2-(carboxymethyl)-3-(4-cyanophenyl)-1-(3-(((((((hexahydro-2-oxo-1H-thieno(3,4-d)imidazol-4-yl)butyl)carbonyl)amino)ethylamino)carbonyl)methyl)-4-methyl-2-oxobenzopyran-7-yl)guanidine; D005456:Fluorescent Dyes; D006146:Guanidines; D007202:Indicators and Reagents; D008024:Ligands; D013549:Sweetening Agents; D001710:Biotin