Molecular basis of film formation from a soybean protein: comparison between the conformation of glycinin in aqueous solution and in films.
Fourier transform infrared spectroscopy has been used to investigate the conformational changes of glycinin. a major storage protein of soybean seeds, upon film-forming. The results show that the secondary structure of glycinin is mainly composed of a beta-sheet (48%) and unordered (49%) structures. The amide I band of glycinin in film-forming conditions, i.e. in alkaline media and in the presence of plasticizing agent, reveals the conversion of 18% of the secondary structure of the protein from the beta-sheet (6%) and random coil (12%) to the alpha-helical conformation due to the helicogenic effect of the ethylene glycol used as the plasticizing agent. Conformational changes also occur upon the film-forming process leading to the formation of intermolecular hydrogen-bonded beta-sheet structures. Results obtained from other plant families indicate that, whatever the origin and conformation of protein, formation of films leads to the appearance of intermolecular hydrogen-bonded beta-sheet structures, suggesting that this type of structure might be essential for the network formation in films. Thus, it is hypothesized that, in the film state, intermolecular hydrogen bonding between segments of beta-sheet may act as junction zones in the film network. This study reveals for the first time that there is a close relationship between the conformation of proteins and the mechanical properties of films.
Subirade
M
M
Centre de Recherches en Sciences et Ingénierie des Macromolécules, Département de Chimie, Université Laval, Qué, Canada.
Kelly
I
I
Guéguen
J
J
Pézolet
M
M
D005916:Globulins / Q000737:chemistry* / Q000187:drug effects; D010968:Plasticizers / Q000737:chemistry / Q000494:pharmacology*; D011487:Protein Conformation; D012996:Solutions; D030262:Soybean Proteins / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014867:Water
D005916:Globulins; D010968:Plasticizers; D012996:Solutions; D030262:Soybean Proteins; D014867:Water; C011474:glycinin