title: HspB3, the most deviating of the six known human small heat shock proteins.
abstract: From the alignment of 14 EST clones, the cDNA sequence of a novel human small heat shock protein (sHsp), called HspB3, could be deduced. The 3' part of the HspB3 cDNA is 99% identical to that of the previously reported HspL27 cDNA (W.Y. Lam, S.K. Wing Tsui, P.T. Law, S.C. Luk, K.P. Fung, C.Y. Lee, M.M. Waye, Isolation and characterization of a human heart cDNA encoding a new member of the small heat shock protein family-HSPL27, Biochim. Biophys. Acta 1314 (1996) 120-124). We argue that the HspB3 cDNA sequence is a corrected version of the HspL27 cDNA. The HspB3 cDNA is 742 bp long and contains an open reading frame specifying a polypeptide of 150 amino acid residues. Among the six known human sHsps it is evident that HspB3 is the most deviating one, having a unique N-terminal domain and essentially lacking a C-terminal extension. Northern blot analysis shows that in smooth muscle tissue the cDNA hybridizes with mRNA of about 0.9 kb.
authors.affiliation: Department of Biochemistry, University of Nijmegen, PO Box 9101, 6500 HB Nijmegen, The Netherlands. w.boelens@bioch.kun.nl
authors.forename: W C
authors.initials: WC
authors.lastname: Boelens
authors.forename: M A
authors.initials: MA
authors.lastname: Van Boekel
authors.forename: W W
authors.initials: WW
authors.lastname: De Jong
mesh_terms: D000595:Amino Acid Sequence; D020224:Expressed Sequence Tags; D006360:Heat-Shock Proteins / Q000737:chemistry*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D016415:Sequence Alignment
chemical_list: C116424:HSPB3 protein, human; D006360:Heat-Shock Proteins; D012333:RNA, Messenger