Hepatoprotective activity of scopoletin, a constituent of Solanum lyratum.
Scopoletin (7-hydroxy-6-methoxycoumarin), a coumarin, was isolated from the aerial part of Solanum lyratum Thunb. by the activity-guided fractionation employing carbon tetrachloride-intoxicated primary cultured rat hepatocytes as a screening system. Its hepatoprotective activity was first evaluated by measuring the release of glutamic pyruvic transaminase and sorbitol dehydrogenase from carbon tetrachloride-intoxicated rat hepatocytes into the culture medium. Scopoletin significantly reduced the releases of glutamic pyruvic transaminase and sorbitol dehydrogenase from the carbon tetrachloride-intoxicated primary cultured rat hepatocytes by 53% and 58%, respectively, from the toxicity in a dose-dependent manner over concentration ranges of 1 microM to 50 microM. Further studies revealed that at the concentration of 10 microM, scopoletin significantly preserved glutathione content by 50% and the activity of superoxide dismutase by 36% and also inhibited the production of malondialdehyde to the degree as seen in the control.
Kang
S Y
SY
College of Pharmacy, Seoul National University, Seoul, Korea.
Sung
S H
SH
Park
J H
JH
Kim
Y C
YC
D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride; D002478:Cells, Cultured; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D019610:Cytoprotection; D007064:L-Iditol 2-Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012603:Scopoletin / Q000737:chemistry / Q000494:pharmacology*; D019657:Solanaceae / Q000737:chemistry
D002251:Carbon Tetrachloride; D007064:L-Iditol 2-Dehydrogenase; D000410:Alanine Transaminase; D012603:Scopoletin