title: Chemo-enzymatic synthesis of calcitonin derivatives containing N-linked oligosaccharides.
abstract: Eel calcitonin derivatives containing various N-linked oligosaccharides were chemo-enzymatically synthesized by the transglycosylation reaction of Mucor hiemalis endo-beta-N-acetylglucosaminidase (Endo-M) to a glycosylated calcitonin derivative [Asn(GlcNac)3]-CT in which N-acetyl-D-glycosamine (GlcNAc) is attached to the L-asparagine (Asn) residue of the peptide.
authors.affiliation: Noguchi Institute, Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Haneda
authors.forename: T
authors.initials: T
authors.lastname: Inazu
authors.forename: M
authors.initials: M
authors.lastname: Mizuno
authors.forename: R
authors.initials: R
authors.lastname: Iguchi
authors.forename: K
authors.initials: K
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Kumagai
authors.forename: S
authors.initials: S
authors.lastname: Aimoto
authors.forename: H
authors.initials: H
authors.lastname: Suzuki
authors.forename: T
authors.initials: T
authors.lastname: Noda
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry; D000595:Amino Acid Sequence; D001216:Asparagine / Q000737:chemistry; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology; D009844:Oligosaccharides / Q000138:chemical synthesis*
chemical_list: D009844:Oligosaccharides; D001216:Asparagine; D002116:Calcitonin; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000117:Acetylglucosamine