Fibroblast growth factor-9 (glia-activating factor) stimulates proliferation and production of glial fibrillary acidic protein in human gliomas either in the presence or in the absence of the endogenous growth factor expression.
We tested fibroblast growth factor-9 (FGF-9) expression in human glioma cells (U251MG, T98G, U87MG, KALS-1, NMC-G1) and only NMC-G1 expressed endogenous FGF-9. All cells expressed bFGF and high affinity receptors for FGFs (FGFR1 and FGFR3). Exogenously supplied bFGF and FGF-9 both showed mitogenic activities in all cells. Neutralizing antibody against bFGF inhibited the proliferation in U251MG and NMC-G1, however that against FGF-9 inhibited the proliferation only in NMC-G1. GFAP expression was stimulated by both FGFs in these cells. FGF-9 potentially regulates proliferation and GFAP expression in human gliomas either in the presence or in the absence of the endogenous growth factor expression.
Miyagi
N
N
Department of Pathology, Kurume University, School of Medicine, Kurume, Japan.
Kato
S
S
Terasaki
M
M
Aoki
T
T
Sugita
Y
Y
Yamaguchi
M
M
Shigemori
M
M
Morimatsu
M
M
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D002455:Cell Division / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000276:immunology / Q000494:pharmacology; D051525:Fibroblast Growth Factor 9; D005346:Fibroblast Growth Factors; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis* / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D006133:Growth Substances / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000187:drug effects
D000911:Antibodies, Monoclonal; C496459:FGF9 protein, human; D051525:Fibroblast Growth Factor 9; D005904:Glial Fibrillary Acidic Protein; D006133:Growth Substances; D009363:Neoplasm Proteins; D016222:Fibroblast Growth Factor 2; D005346:Fibroblast Growth Factors