Preventive effects of germinated barley foodstuff on methotrexate-induced enteritis in rats.
The preventive effects of the dietary germinated barley foodstuff (GBF), which increases the contents of protein, RNA and DNA in the intestinal mucosa of rats on the mucosal damage and diarrhea were examined in a methotrexate (MTX)-induced enteritis model in rats. Sprague-Dawley rats intraperitoneally injected with MTX (10 mg/kg body weight) were used as an enteritis model. After consumption of diets containing GBF, glutamine or a glutamine-rich stuff (gluten), mucosal damage, contents of mucosal protein, RNA and DNA, myeloperoxidase (MPO) activity, bacterial translocation and DNA synthetic activity in the small intestine were assessed. GBF more effectively prevented diarrhea and mucosal damages, and increased mucosal protein, DNA and RNA contents than glutamine or gluten. The bacterial trans-location and elevation of MPO activity induced by MTX were depressed only by the consumption of GBF. GBF has a potential as therapeutic diet to decrease the adverse effects of anti-cancer chemotherapy.
Kanauchi
O
O
Applied Bioresearch Center, Corporate Research and Development Division, Kirin Brewery Co. Ltd., Takasaki, Gunma 370-12, Japan.
Mitsuyama
K
K
Saiki
T
T
Agata
K
K
Nakamura
T
T
Iwanaga
T
T
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004247:DNA / Q000187:drug effects / Q000378:metabolism; D003967:Diarrhea / Q000139:chemically induced / Q000517:prevention & control; D004043:Dietary Fiber / Q000008:administration & dosage*; D004435:Eating / Q000187:drug effects; D004751:Enteritis / Q000139:chemically induced / Q000517:prevention & control*; D018525:Germination; D001467:Hordeum / Q000737:chemistry* / Q000254:growth & development; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D008727:Methotrexate / Q000633:toxicity*; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D012313:RNA / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013393:Sucrase / Q000187:drug effects / Q000378:metabolism
D004043:Dietary Fiber; D011506:Proteins; D012313:RNA; D004247:DNA; D009195:Peroxidase; D013393:Sucrase; D008727:Methotrexate