Human proteolipid protein (PLP) mediates winding and adhesion of phospholipid membranes but prevents their fusion.
Proteolipid protein (PLP or lipophilin) is a highly conserved, strongly hydrophobic, integral membrane protein, and is the major protein component of central nervous system myelin. Although PLP has been implicated in many functions, its in vivo role is still uncertain. Here, we report the investigation of PLP's putative adhesive function using purified PLP and reconstituted phospholipid vesicles made of either 100% phosphatidylcholine (PC), or a mixture of 92% PC and 8% phosphatidylserine (PS), by weight. PLP-induced changes in the phospholipid bilayer surfaces were directly examined by transmission electron microscopy. We found that upon the introduction of PLP, larger lipid vesicles became smaller and unilamellar. At the PLP:lipid molar ratio of 1:20, vesicle membranes rolled onto themselves forming 'croissant'-like structures that subsequently adhered to each other. The phenomena of PLP-induced bilayer rolling and adhesion were dependent on the concentration of PLP and the period of incubation, but were independent of the presence of calcium and types of phospholipids (PC or PC:PS). Furthermore, the presence of PLP in the lipid bilayers prevented the fusion of membranes. These findings show that PLP can induce membrane 'winding' while preventing the fusion of adjacent lipid bilayers. Hence, our data provide direct evidence for PLP's suspected function of membrane adhesion, and also suggest that PLP could potentially play a role in the formation of the myelin sheath.
Palaniyar
N
N
Department of Molecular Biology and Genetics, The University of Guelph, 50 Stone Road East, Guelph, Ont. N1G 2W1, Canada.
Semotok
J L
JL
Wood
D D
DD
Moscarello
M A
MA
Harauz
G
G
D001059:Apoproteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D005614:Freeze Fracturing; D006801:Humans; D008051:Lipid Bilayers; D008854:Microscopy, Electron; D018991:Myelin Proteolipid Protein / Q000378:metabolism*; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding
D001059:Apoproteins; D008051:Lipid Bilayers; D018991:Myelin Proteolipid Protein; D010743:Phospholipids; D002118:Calcium