Alterations in cholesterol sulfate and its biosynthetic enzyme during multistage carcinogenesis in mouse skin.
Recent evidence suggests that cholesterol sulfate may be an important second messenger involved in signaling epidermal differentiation in skin. The activity of cholesterol sulfotransferase (Ch-ST) is increased during squamous differentiation of keratinocytes and is believed to be a marker enzyme for terminal differentiation. The primary objective of this study was to examine changes in levels of cholesterol sulfate (CS) and activity of its biosynthetic enzyme, Ch-ST, during multistage carcinogenesis in mouse skin. Using SENCAR mice, we determined the activity of Ch-ST in normal epidermis, in tumor promoter-treated epidermis, in epidermis during wound healing, and in mouse skin tumors generated by initiation-promotion regimens. A single topical application of tumor promoters led to significantly elevated levels of Ch-ST activity and of CS. Epidermal Ch-ST activity was also elevated during wound healing. Dramatic increases in CS levels and in the activity of Ch-ST were found in nearly all of the papillomas and squamous cell carcinomas examined. The increased levels of CS and activity of Ch-ST in tumor promoter-treated epidermis were accompanied by increased transglutaminase-I activity. In contrast, transglutaminase I activity was not elevated in primary papillomas or squamous cell carcinomas. Finally, Ch-ST activity was significantly elevated in the epidermis of newborn HK1.ras transgenic mice, whereas transglutaminase I activity did not correlate with Ch-ST activity in these mice. These results demonstrate that diverse tumor-promoting stimuli all produce elevated CS levels and Ch-ST activity and that CS levels and Ch-ST activity were constitutively elevated in both papillomas and squamous cell carcinomas. The data also suggest a mechanism for upregulation of Ch-ST in skin tumors involving activation/upregulation of Ha-ras.
Kiguchi
K
K
Department of Carcinogenesis, The University of Texas, M.D. Anderson Cancer Center, Smithville 78957, USA.
Kagehara
M
M
Higo
R
R
Iwamori
M
M
DiGiovanni
J
J
D000287:Administration, Topical; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D016588:Anticarcinogenic Agents / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002788:Cholesterol Esters / Q000378:metabolism*; D019314:Dehydroepiandrosterone Sulfate / Q000494:pharmacology; D004817:Epidermis / Q000187:drug effects / Q000201:enzymology; D005260:Female; D015603:Keratinocytes / Q000166:cytology; D051379:Mice; D018632:Mice, Inbred SENCAR; D008822:Mice, Transgenic; D019319:Okadaic Acid / Q000494:pharmacology; D012878:Skin Neoplasms / Q000201:enzymology* / Q000209:etiology / Q000378:metabolism*; D015238:Sulfotransferases / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D011503:Transglutaminases / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology
D000873:Anthracenes; D016588:Anticarcinogenic Agents; D002273:Carcinogens; D002788:Cholesterol Esters; D019319:Okadaic Acid; D019314:Dehydroepiandrosterone Sulfate; C013883:chrysarobin; D011503:Transglutaminases; D015238:Sulfotransferases; C043456:cholesterol sulfotransferase; C007045:cholesteryl sulfate; D013755:Tetradecanoylphorbol Acetate