In vivo monitoring of gabapentin in rats: a microdialysis study coupled to capillary electrophoresis and laser-induced fluorescence detection.
Gabapentin (GP) is a new anticonvulsant used in refractory epilepsy. Few studies have monitored the drug in vivo. We report the combination of capillary electrophoresis and laser-induced fluorescence detection (CZE-LIFD) with brain microdialysis and plasma ultrafiltration in an attempt to measure GP and offer an alternative technique for pharmacokinetic studies. We found that CZE-LIFD is capable of linearly measuring 10(-7)-10(-9) M GP in a 1 nL volume. It was also demonstrated that it is possible to monitor GP in prefrontal cortex dialysates and plasma in rats. It is concluded that the method permits in vivo monitoring of the drug in pharmacological as well as in clinical studies.
Rada
P
P
Laboratory of Behavioral Physiology, University of Los Andes, Mérida, Venezuela. radap@ing.ula.ve
Tucci
S
S
Pérez
J
J
Teneud
L
L
Chuecos
S
S
Hernández
L
L
D000085:Acetates / Q000097:blood / Q000493:pharmacokinetics*; D000588:Amines; D000818:Animals; D000927:Anticonvulsants / Q000097:blood / Q000493:pharmacokinetics*; D001921:Brain / Q000378:metabolism*; D003509:Cyclohexanecarboxylic Acids; D019075:Electrophoresis, Capillary; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D000077206:Gabapentin; D006440:Hemofiltration; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar; D005680:gamma-Aminobutyric Acid
D000085:Acetates; D000588:Amines; D000927:Anticonvulsants; D003509:Cyclohexanecarboxylic Acids; D005456:Fluorescent Dyes; D005680:gamma-Aminobutyric Acid; D000077206:Gabapentin; D016650:Fluorescein-5-isothiocyanate