title: Reparative dentin formation in rat molars after direct pulp capping with growth factors.
abstract: Growth factors involved in normal wound healing may promote tissue repair when applied as a direct pulp capping medication. A minimal pulp exposure was made in rat molars, a pulp capping medication was placed, and the cavity was sealed. Epidermal growth factor, basic fibroblast growth factor, insulin-like growth factor II, platelet-derived growth factor-BB, and transforming growth factor-beta 1 (TGF-beta 1)--each absorbed onto a sterile collagen membrane (BioMend; Calcitek, Carlsbad, CA)--were used separately as pulpal medicaments. Dycal, unimpregnated collagen membrane, and no medication were used as controls. Eight samples from each treatment regimen were collected 2 and 3 weeks after surgery. Pulpal soft and hard tissue responses were graded. Data were analyzed by one-way ANOVA and Tukey-Kramer tests. No significant differences were detected after 2 wk. Pulp treated with TGF-beta 1 showed significantly improved soft and hard tissue healing at week 3, compared with the procedure control. We conclude that TGF-beta 1 as a pulp-capping medication enhances reparative dentin formation in rat molars.
authors.affiliation: Department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio 78284-7888, USA.
authors.forename: C C
authors.initials: CC
authors.lastname: Hu
authors.forename: C
authors.initials: C
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Qian
authors.forename: N B
authors.initials: NB
authors.lastname: Tatum
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003782:Dental Pulp / Q000187:drug effects*; D003785:Dental Pulp Capping / Q000379:methods*; D003809:Dentin, Secondary / Q000187:drug effects / Q000254:growth & development; D003810:Dentinogenesis / Q000187:drug effects; D006133:Growth Substances / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects
chemical_list: D006133:Growth Substances; D016212:Transforming Growth Factor beta