[Studies on selectivity of recognizing factor for rmf promoter].
The truncted DNA template carrying rmf gene promoter was transcripted by E. coli RNA polymorase holoenzyme (E sigma) reconstituted with core enzyme (alpha 2 beta beta') and sigma 70 or sigma 38 in vitro. The initional site of the transcription of rmf was confirmed with different restriction endonuclease. rmf promoter can be recognized by E sigma 70 but not E sigma 38. The suitable temperature for in vitro transcription was 37 degrees C, NaCl concentration was 50 mmol/L.
Ding
Q
Q
Wuhan Institute of Virology, Academia Sinica.
Akira
I
I
D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D029968:Escherichia coli Proteins; D005796:Genes; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012269:Ribosomal Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic
D029968:Escherichia coli Proteins; D012269:Ribosomal Proteins; C063545:ribosome modulation factor, E coli; D012321:DNA-Directed RNA Polymerases