Cloning and analysis of the Epstein-Barr virus glycoprotein 350 genes.
Membrane glycoprotein 350 (gp350) of the Epstein-Barr virus (EBV) is considered as a major target for vaccine development, since the gp350 has been identified as the virus' mediator for receptor interaction and as an inducer of specific in vitro virus-neutralizing antibodies. In an initial attempt to develop an effective DNA vaccine against an EBV infection, gp350 genes were isolated from SNU-20 and SNU-1103 which are the EBV-infected lymphoblastoid cell lines established in Korea. In addition, the nucleotide sequences of the gp350 genes were determined and compared with those of other EBV strains such as B95-8, P3HR-1/AG876 and M81. Sequence analysis showed that similar high degrees of homology between 2 EBV strains derived from African Burkitt's lymphoma, P3HR-1 and AG876, was shown between the gp350 genes isolated from 2 EBV-infected lymphoblastoid cell lines established in Korea. Furthermore, these 2 Korean and 2 African strains displayed nearly identical patterns of sequence variations from B95-8. In addition, the sequence of the isolated gp350 genes, which have been reported to be associated with the biology of EBV infection, is analyzed.
Chang
S H
SH
Faculty of Biological Sciences and The Institute for Molecular Biology and Genetics, Chonbuk National University, Chonju, Korea.
Kim
S H
SH
Lee
W K
WK
Kim
H J
HJ
Choi
S H
SH
Park
J H
JH
Jang
H S
HS
Chung
G H
GH
Kwon
T H
TH
Kim
D H
DH
Yang
M S
MS
Jang
Y S
YS
D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D014763:Viral Matrix Proteins / Q000235:genetics* / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics*
D000956:Antigens, Viral; D004279:DNA, Viral; C034059:GP 300-350, Epstein-Barr virus; D008562:Membrane Glycoproteins; D014763:Viral Matrix Proteins; D015678:Viral Structural Proteins