title: Substrate specificity of thermostable farnesyl diphosphate synthase with alkyl group homologs of isopentenyl diphosphate.
abstract: 3-Alkyl group homologs of isopentenyl diphosphate were examined for the reactivity as substrates of the thermostable farnesyl diphosphate (FPP) synthase of Bacillus stearothermophilus. Even 3-n-propyl- and 3-n-butyl-but-3-enyl diphosphates, which are hardly acceptable by animal FPP synthases, are accepted by this bacterial enzyme as substrates to react with dimethylallyl- and geranyl diphosphates, yielding 7-methyl-3-n-propylocta-2,6-dienyl- and 7,11-dimethyl-3-n-propyldodeca-2,6,10-trienyl diphosphate, respectively.
authors.affiliation: Department of Materials Science and Technology, Faculty of Science and Technology, Hirosaki University, Aomori, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Nagaki
authors.forename: H
authors.initials: H
authors.lastname: Kannari
authors.forename: J
authors.initials: J
authors.lastname: Ishibashi
authors.forename: Y
authors.initials: Y
authors.lastname: Maki
authors.forename: T
authors.initials: T
authors.lastname: Nishino
authors.forename: K
authors.initials: K
authors.lastname: Ogura
authors.forename: T
authors.initials: T
authors.lastname: Koyama
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000378:metabolism*; D000478:Alkylation; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D001411:Geobacillus stearothermophilus; D051228:Geranyltranstransferase; D045782:Hemiterpenes; D008099:Liver / Q000201:enzymology; D009943:Organophosphorus Compounds / Q000737:chemistry* / Q000378:metabolism; D013379:Substrate Specificity; D013552:Swine
chemical_list: D000942:Antigens, Bacterial; D045782:Hemiterpenes; D009943:Organophosphorus Compounds; C004809:isopentenyl pyrophosphate; D019883:Alkyl and Aryl Transferases; D051228:Geranyltranstransferase