Histodifferentiation of hair follicles in grafting of cell aggregates obtained by rotation culture of embryonic rat skin.
We have previously reported reconstruction of hair follicles from a single cell suspension of rat fetal upper lip by a two-step culture method consisting of rotation and flotation cultures. Rotation sorted out the cells and flotation facilitated histodifferentiation. In the present study, we added grafting procedures to the previous method to see whether cell aggregates obtained this way were graftable, and whether the grafting promoted histodifferentiation. The aggregates before and after flotation were grafted, and differentiation of hair follicles comparable to those in vivo was confirmed 10 days after grafting. There was no difference in the degree of differentiation between the two kinds of grafts. The grafting procedure therefore resulted in an appreciable increase in histodifferentiation even when aggregates obtained after flotation were grafted.
Takeda
A
A
Department of Plastic and Reconstructive Surgery, Kitasato University School of Medicine, Kanagawa, Japan.
Matsuhashi
S
S
Shioya
N
N
Ihara
S
S
D000818:Animals; D002449:Cell Aggregation; D002454:Cell Differentiation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D018859:Hair Follicle / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012399:Rotation; D012867:Skin / Q000166:cytology* / Q000196:embryology