On the preparative use of recombinant pig alpha(1-3)galactosyl-transferase.
A series of non-natural N-acyl derivatives of lactosamine is incubated with recombinant alpha(1-3)galactosyl-transferase and UDP-galactose. The enzyme shows a high promiscuity towards the non-natural acceptors. It selectively transfers a galactose unit onto the 3-OH group of the terminal beta-linked galactose in an alpha-mode to give an array of linear-B trisaccharides.
Baisch
G
G
Novartis Pharma AG, Basle, Switzerland.
Ohrlein
R
R
Kolbinger
F
F
Streiff
M
M
D000818:Animals; D005700:Galactosyltransferases / Q000378:metabolism*; D006031:Glycosylation; D009682:Magnetic Resonance Spectroscopy; D011994:Recombinant Proteins / Q000378:metabolism; D013552:Swine
D011994:Recombinant Proteins; D005700:Galactosyltransferases; C037959:beta-D-galactosyl-N-acetylglucosaminylglycopeptide alpha-1,3-galactosyltransferase