New mouse model for dengue virus vaccine testing.
Several dengue (DEN) virus vaccines are in development; however, the lack of a reliable small animal model in which to test them is a major obstacle. Because evidence suggests that interferon (IFN) is involved in the human anti-DEN virus response, we tested mice deficient in their IFN functions as potential models. Intraperitoneally administered mouse-adapted DEN 2 virus was uniformly lethal in AG129 mice (which lack alpha/beta IFN and gamma IFN receptor genes), regardless of age. Immunized mice were protected from virus challenge, and survival times increased following passive transfer of anti-DEN polyclonal antibody. These results demonstrate that AG129 mice are a promising small animal model for DEN virus vaccine trials.
Johnson
A J
AJ
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, United States Department of Health and Human Services, Fort Collins, Colorado 80522, USA. ajj1@ckc.goN
Roehrig
J T
JT
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000097:blood*; D003716:Dengue Virus / Q000276:immunology*; D007114:Immunization; D007371:Interferon-gamma / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D017471:Receptors, Interferon / Q000502:physiology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D000096743:Interferon gamma Receptor
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007371:Interferon-gamma; D017471:Receptors, Interferon; D014765:Viral Vaccines; D000096743:Interferon gamma Receptor