CC chemokine receptors, CCR-1 and CCR-3, are potentially involved in antigen-presenting cell function of human peripheral blood monocyte-derived dendritic cells.
We examined the potential involvement of two CC chemokine receptors (CCRs), CCR-1 and CCR-3, in the functional activation of granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interleukin-4 (IL-4)-generated human peripheral blood monocyte-derived immature dendritic cells (DCs). Flow cytometric analysis showed that CCR-1, CCR-3, CCR-5, and CXC chemokine receptor (CXCR)-4 were expressed on the cell surface of monocyte-derived DCs. Treatment with a monoclonal antibody (MoAb) to either CCR-1 or CCR-3 but not MoAbs to CCR-5 and CXCR-4 abolished chemotactic migration of monocyte-derived DCs. The DCs treated with either the anti-CCR-1 MoAb or anti-CCR-3 MoAb were less efficient than untreated DCs in proliferation of allogeneic T cells (TCs) and TC-derived secretion of interferon-gamma (IFN-gamma). The homotypic aggregation of DCs and heterotypic aggregation of DCs with TCs were suppressed by the anti-CCR-1 MoAb or anti-CCR-3 MoAb. These results indicate that CCR-1 and CCR-3 specifically regulate interaction of TCs and DCs in the process of antigen presentation.
Sato
K
K
Department of Cell Processing, Department of Clinical Immunology and AIDS Research Center, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Kawasaki
H
H
Nagayama
H
H
Serizawa
R
R
Ikeda
J
J
Morimoto
C
C
Yasunaga
K
K
Yamaji
N
N
Tadokoro
K
K
Juji
T
T
Takahashi
T A
TA
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000938:Antigen-Presenting Cells / Q000502:physiology*; D002454:Cell Differentiation; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000378:metabolism*; D002633:Chemotaxis / Q000276:immunology; D003713:Dendritic Cells / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D009000:Monocytes / Q000378:metabolism / Q000502:physiology*; D054389:Receptors, CCR1; D054397:Receptors, CCR3; D019707:Receptors, Chemokine / Q000096:biosynthesis / Q000276:immunology / Q000502:physiology*; D013601:T-Lymphocytes / Q000276:immunology
D000911:Antibodies, Monoclonal; C515300:CCR1 protein, human; C515350:CCR3 protein, human; D019742:Chemokines, CC; D054389:Receptors, CCR1; D054397:Receptors, CCR3; D019707:Receptors, Chemokine