title: Centrifugal pulsing increases the recovery of viable spermatozoa.
abstract: OBJECTIVE
  Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.
  
  
  METHODS
  A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk results determined the efficacy of the centrifugation techniques.
  
  
  RESULTS
  The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.
  
  
  CONCLUSIONS
  Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.
authors.affiliation: Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Bielfeld
authors.forename: V
authors.initials: V
authors.lastname: Acosta
authors.forename: R S
authors.initials: RS
authors.lastname: Jeyendran
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012661:Semen / Q000166:cytology*; D013081:Sperm Motility; D013094:Spermatozoa