title: Adhesion of oral Candida albicans isolates to denture acrylic following limited exposure to antifungal agents.
abstract: Candidal adherence to denture acrylic surfaces is implicated as the first step in the pathogenesis of Candida-associated denture stomatitis, the most prevalent form of oral candidosis in the West. This condition is treated by topically administered antifungal agents, mainly belonging to the polyenes and azoles. As the intraoral concentrations of antifungals fluctuate considerably due to the dynamics of the oral environment, the effect of short exposure to sublethal concentrations of antifungals on the adhesion of Candida albicans to denture acrylic surfaces was investigated. Seven oral C. albicans isolates were exposed to four-eight times minimum inhibitory concentrations (MIC) of five antifungal drugs, nystatin, amphotericin B, 5-fluorocytosine, ketoconazole and fluconazole, for 1 h. After removing the drug (by repeated washing) the adhesion of these isolates to acrylic strips was assessed by an in vitro adhesion assay. Exposure to antifungal agents significantly reduced the adherence of all seven C. albicans isolates to denture acrylic. The mean percentage reductions of adhesion after limited exposure to nystatin, amphotericin B, 5-fluorocytosine, ketoconazole and fluconazole were 86.48, 90.85, 66.72, 65.88 and 47.42%, respectively. These findings indicate that subtherapeutic doses of antifungals may modulate oral candidal colonization. Further, these results may have an important bearing on dosage regimens currently employed in treating oral candidosis.
authors.affiliation: Faculty of Dentistry, University of Hong Kong, Hong Kong.
authors.forename: A N
authors.initials: AN
authors.lastname: Ellepola
authors.forename: L P
authors.initials: LP
authors.lastname: Samaranayake
mesh_terms: D000268:Adhesiveness; D000287:Administration, Topical; D000666:Amphotericin B / Q000008:administration & dosage / Q000494:pharmacology; D000704:Analysis of Variance; D000935:Antifungal Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002176:Candida albicans / Q000145:classification / Q000187:drug effects* / Q000648:ultrastructure; D002180:Candidiasis, Oral / Q000188:drug therapy; D003819:Denture Bases; D004334:Drug Administration Schedule; D015725:Fluconazole / Q000008:administration & dosage / Q000494:pharmacology; D005437:Flucytosine / Q000008:administration & dosage / Q000494:pharmacology; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007654:Ketoconazole / Q000008:administration & dosage / Q000494:pharmacology; D009055:Mouth / Q000382:microbiology; D009761:Nystatin / Q000008:administration & dosage / Q000494:pharmacology; D019904:Polymethyl Methacrylate / Q000737:chemistry; D013282:Stomatitis, Denture / Q000188:drug therapy / Q000382:microbiology; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000935:Antifungal Agents; D009761:Nystatin; D000666:Amphotericin B; D015725:Fluconazole; D019904:Polymethyl Methacrylate; D005437:Flucytosine; D007654:Ketoconazole