Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem.
CmPP16 from Cucurbita maxima was cloned and the protein was shown to possess properties similar to those of viral movement proteins. CmPP16 messenger RNA (mRNA) is present in phloem tissue, whereas protein appears confined to sieve elements (SE). Microinjection and grafting studies revealed that CmPP16 moves from cell to cell, mediates the transport of sense and antisense RNA, and moves together with its mRNA into the SE of scion tissue. CmPP16 possesses the characteristics that are likely required to mediate RNA delivery into the long-distance translocation stream. Thus, RNA may move within the phloem as a component of a plant information superhighway.
Xoconostle-Cázares
B
B
Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616, USA.
Xiang
Y
Y
Ruiz-Medrano
R
R
Wang
H L
HL
Monzer
J
J
Yoo
B C
BC
McFarland
K C
KC
Franceschi
V R
VR
Lucas
W J
WJ
D000595:Amino Acid Sequence; D001692:Biological Transport; D003001:Cloning, Molecular; D018553:Cucumis sativus; D019662:Cucurbitaceae / Q000235:genetics / Q000378:metabolism*; D008845:Microinjections; D008969:Molecular Sequence Data; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism; D018547:Plant Stems / Q000378:metabolism; D053830:Plant Viral Movement Proteins; D016372:RNA, Antisense / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D018749:RNA, Plant / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism
C116564:PP16 protein, Cucurbita maxima; D010940:Plant Proteins; D053830:Plant Viral Movement Proteins; D016372:RNA, Antisense; D012333:RNA, Messenger; D018749:RNA, Plant; D014764:Viral Proteins