T lymphocytes, B lymphocytes, and macrophages in the ovaries and oviducts of laying hens experimentally infected with Salmonella enteritidis.
Subsets of T lymphocytes, B lymphocytes, and macrophages in the ovaries and oviducts of laying hens were enumerated by immunohistochemistry after intravenous inoculation with Salmonella enteritidis. Almost all T cell subsets in the ovaries and different regions of the oviduct increased in number at 7 days post-inoculation and reached a peak by day 10. This T cell surge was followed by a peak in B cell numbers at day 14. The number of macrophages declined initially but recovered to preinoculation levels by day 21. At day 21, the numbers of T and B cells also returned to normal levels, except for IgG+ B cells in the infundibulum, isthmus, and vagina where they remained consistently elevated. The T and B cell proliferation at 10-14 days post-inoculation immediately preceded a decline in the number of S. enteritidis positive tissues from infected hens beginning at day 14 suggesting that these lymphocytes play a major role in the local immune response to S. enteritidis. The Salmonella-oviduct model will be useful for future studies on local immunity to various infectious agents.
Withanage
G S
GS
Department of Veterinary Science, College of Agriculture Osaka Prefecture University Sakai, Japan.
Sasai
K
K
Fukata
T
T
Miyamoto
T
T
Baba
E
E
Lillehoj
H S
HS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology; D002645:Chickens; D005260:Female; D007150:Immunohistochemistry; D007275:Injections, Intravenous / Q000662:veterinary; D008264:Macrophages / Q000276:immunology / Q000382:microbiology; D008856:Microscopy, Fluorescence / Q000662:veterinary; D010053:Ovary / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D010057:Oviducts / Q000166:cytology / Q000276:immunology* / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology
D000911:Antibodies, Monoclonal