On the preparative use of recombinant beta(1-3)galactosyl-transferase.
A number of non-natural N-acyl derivatives of glucosamine is incubated with a recombinant beta(1-3)galactosyl-transferase and UDP-galactose. Surprisingly, the enzyme recognizes the non-natural acceptors as substrates and transfers galactose onto the 3-OH group in a beta-mode to give a series of Lewis(c)-(type 1) disaccharides.
Baisch
G
G
Novartis Pharma AG, Schwarzwaldallee, Basle, Switzerland.
Ohrlein
R
R
Streiff
M
M
Kolbinger
F
F
D002236:Carbohydrate Conformation; D004187:Disaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D005690:Galactose; D005700:Galactosyltransferases / Q000378:metabolism*; D005944:Glucosamine / Q000031:analogs & derivatives; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014531:Uridine Diphosphate Galactose
D004187:Disaccharides; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D014531:Uridine Diphosphate Galactose; D005700:Galactosyltransferases; D005944:Glucosamine; D005690:Galactose