Uptake and incorporation of choline and ethanolamine into lipoteichoic acid and teichoic acid by the choline-independent mutant JY2190 of Streptococcus pneumoniae.
Lipoteichoic acid- and teichoic acid-containing muropeptides were isolated from choline- or ethanolamine-grown cells of the choline-independent mutant JY2190 of Streptococcus pneumoniae. Choline was taken up and incorporated into lipoteichoic acid and teichoic acid with 81% efficiency, compared with the parent strain Rx1. With similar efficiency, ethanolamine was incorporated. Accordingly, the mutant is a valuable tool for identifying the individual genes encoding the enzymes of choline utilisation, because any of these genes can be deleted without affecting viability and growth rate.
Leopold
K
K
Institut für Biochemie, Med. Fak., Universität Erlangen-Nürnberg, Germany.
Fischer
W
W
D002794:Choline / Q000235:genetics / Q000378:metabolism*; D002846:Chromatography, Affinity; D019856:Ethanolamine / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism; D009154:Mutation; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000378:metabolism*; D013682:Teichoic Acids / Q000378:metabolism*
D008070:Lipopolysaccharides; D013682:Teichoic Acids; C009900:lipoteichoic acid; D019856:Ethanolamine; D002794:Choline