Herpes simplex virus genome isomerization: origins of adjacent long segments in concatemeric viral DNA.
Herpes simplex virus type 1 DNA isomerization was studied by using a viral mutant, 5B8, lacking the unique SpeI site of its parent, SC16. In coinfected cells, SC16 genomic long segments flanked 5B8 genomes in all possible orientations with similar frequencies. Thus, recombination between progeny of different replication templates is sufficient to explain genomic isomerization.
Slobedman
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B
Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia.
Zhang
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X
Simmons
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A
D000818:Animals; D002522:Chlorocebus aethiops; D004261:DNA Replication; D004270:DNA, Circular / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry*; D016679:Genome, Viral; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000235:genetics*; D014709:Vero Cells; D014779:Virus Replication
D004270:DNA, Circular; D004279:DNA, Viral