title: Effects of water deprivation and angiotensin II intracerebroventricular administration on brain nitric oxide synthase activity.
abstract: Intracranial administration of L-arginine causes a reduction of the water intake induced by water deprivation or by intracerebroventricular (i.c.v.) injection of angiotensin II (angiotensin II), through the release of nitric oxide (NO) in the central nervous system. We studied the effects of i.c.v. angiotensin II (120 ng/rat) in association with i.c.v. L-arginine (2.5-10 microg/rat) on blood pressure. We also studied the effects of both peripheral and central angiotensin II injection (1.5-6 mg kg(-1) i.p. and 30-120 ng rat(-1) i.c.v., respectively) on NO synthase activity in the cortex, diencephalon and brainstem, after water deprivation (24 h), conditions producing activation of the renin-angiotensin system. L-arginine dose dependently antagonized the increase in blood pressure induced by i.c.v. angiotensin II (P < 0.001). Peripheral administration of angiotensin II produced a dose-dependent reduction of NO synthase activity in the brainstem and cortex (P < 0.001), but not in the diencephalon. Water deprivation produced similar effects on brain NO synthase activity. Angiotensin II i.c.v. injection caused NO synthase activity reduction in all brain regions studied (P < 0.001). Our findings suggest that NO and angiotensin II could play opposite roles in brain regulation of blood pressure and drinking behaviour.
authors.affiliation: Institute of Pharmacology, School of Medicine, University of Messina, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Calapai
authors.forename: M C
authors.initials: MC
authors.lastname: Marciano
authors.forename: G
authors.initials: G
authors.lastname: Costantino
authors.forename: A
authors.initials: A
authors.lastname: Russo
authors.forename: F
authors.initials: F
authors.lastname: Corica
authors.forename: L
authors.initials: L
authors.lastname: Sautebin
authors.forename: M
authors.initials: M
authors.lastname: Di Rosa
authors.forename: A P
authors.initials: AP
authors.lastname: Caputi
mesh_terms: D000804:Angiotensin II / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology; D004027:Diencephalon / Q000187:drug effects / Q000201:enzymology; D004333:Drug Administration Routes; D004789:Enzyme Activation; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D014868:Water Deprivation
chemical_list: D000804:Angiotensin II; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat