title: Synthesis of 2-deoxy-2-fluoro-phosphatidylinositol-4,5-bisphosphate and analogues: probes and modulators of the mammalian PI-PLCS.
abstract: An approach to synthesis of 2-modified phosphatidylinositol-4,5-bisphosphates, which are substrate analogues useful as probes and modulators of the PI-PLC enzyme family, is described and illustrated for the dibutyl-2-deoxy-2-fluoro analogue, a probe designed for delineating substrate and PI-PLC interactions by X-ray crystallography.
authors.affiliation: Functional Lipids Division, Nutrimed Biotech, Langmuir Laboratory, Ithaca, NY 14850, USA.
authors.forename: S G
authors.initials: SG
authors.lastname: Aneja
authors.forename: P T
authors.initials: PT
authors.lastname: Ivanova
authors.forename: R
authors.initials: R
authors.lastname: Aneja
mesh_terms: D000818:Animals; D018360:Crystallography, X-Ray; D015195:Drug Design; D007202:Indicators and Reagents; D008322:Mammals; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010738:Type C Phospholipases / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
chemical_list: D007202:Indicators and Reagents; D019269:Phosphatidylinositol 4,5-Diphosphate; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase