Enzymatic fucosylations of non-natural sialylated type-I trisaccharides with recombinant fucosyl-transferase-III.
Recombinant fucosyl-transferase-III (Lewis type enzyme) is used to prepare a series of non-natural sialyl-Lewis derivatives on a preparative scale. The enzyme tolerates a wide range of acceptors which have the natural N-acetyl group of the glucosamine moiety replaced by substituted aromatic and heteroaromatic amides.
Baisch
G
G
Novartis Pharma AG, Basle, Switzerland.
Ohrlein
R
R
Streiff
M
M
D002240:Carbohydrate Sequence; D005647:Fucosyltransferases / Q000737:chemistry*; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D009844:Oligosaccharides / Q000737:chemistry*; D011994:Recombinant Proteins / Q000737:chemistry
D009844:Oligosaccharides; D011994:Recombinant Proteins; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase; D019158:N-Acetylneuraminic Acid