[Conditions for the overexpressions of copper--resistant proteins from Escherichia coli].
There were five copper--resistant proteins encoded in the copper resistant plasmid pRJ1004 from Escherichia coli. The conditions for the overexpressions of two of the five, PcoC and PcoE, were reported in this paper. For the overexpression of PcoC using IPTG (Isopropyl-beta-D-thiogalactoside) as inducer, the best conditions were: medium, LB; IPTG concentration, 0.1 mmol/L; inducing time, one and half hours. For the overexpression of PcoE using CuSO4 as inducer, the best conditions were: medium, LB; CuSO4 concentration, 2.0 mmol/L; inducing time, one and halfhours.
Liu
Z
Z
Institute of Microbiology, Academia Sinica, Beijing.
Brown
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D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D003300:Copper / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D003300:Copper