title: Identification of alpha-dystroglycan as a receptor for lymphocytic choriomeningitis virus and Lassa fever virus.
abstract: A peripheral membrane protein that is interactive with lymphocytic choriomeningitis virus (LCMV) was purified from cells permissive to infection. Tryptic peptides from this protein were determined to be alpha-dystroglycan (alpha-DG). Several strains of LCMV and other arenaviruses, including Lassa fever virus (LFV), Oliveros, and Mobala, bound to purified alpha-DG protein. Soluble alpha-DG blocked both LCMV and LFV infection. Cells bearing a null mutation of the gene encoding DG were resistant to LCMV infection, and reconstitution of DG expression in null mutant cells restored susceptibility to LCMV infection. Thus, alpha-DG is a cellular receptor for both LCMV and LFV.
authors.affiliation: Division of Virology, Department of Neuropharmacology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: W
authors.initials: W
authors.lastname: Cao
authors.forename: M D
authors.initials: MD
authors.lastname: Henry
authors.forename: P
authors.initials: P
authors.lastname: Borrow
authors.forename: H
authors.initials: H
authors.lastname: Yamada
authors.forename: J H
authors.initials: JH
authors.lastname: Elder
authors.forename: E V
authors.initials: EV
authors.lastname: Ravkov
authors.forename: S T
authors.initials: ST
authors.lastname: Nichol
authors.forename: R W
authors.initials: RW
authors.lastname: Compans
authors.forename: K P
authors.initials: KP
authors.lastname: Campbell
authors.forename: M B
authors.initials: MB
authors.lastname: Oldstone
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018050:Arenavirus / Q000378:metabolism; D002460:Cell Line; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D049030:Dystroglycans; D007836:Lassa virus / Q000378:metabolism* / Q000502:physiology; D008217:Lymphocytic choriomeningitis virus / Q000378:metabolism* / Q000502:physiology; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D011991:Receptors, Virus / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D003598:Cytoskeletal Proteins; D008562:Membrane Glycoproteins; D011991:Receptors, Virus; D011993:Recombinant Fusion Proteins; D049030:Dystroglycans