title: Altered localization of E-cadherin and alpha-catenin in rat esophageal tumors.
abstract: An alteration in the localization of E-cadherin and its associated proteins has been observed in many epithelial neoplasms. No data exist, however, for the expression of these proteins in an animal model system for esophageal cancer or in cultured rat esophageal epithelial cell lines. The present study investigated the localization of E-cadherin and its associated protein, alpha-catenin, in rat esophageal epithelial cell lines of differing tumorigenic potential; in tumors induced after transplantation of these cell lines into syngeneic hosts; and, in esophageal tumors induced in rats by the carcinogen, N-nitrosomethylbenzylamine (NMBA). Immunofluorescent staining of the cultured cell lines revealed staining for both E-cadherin and alpha-catenin at cell-cell boundaries. Western blot analysis confirmed the membrane-bound localization of E-cadherin and alpha-catenin in the cells. However, tumors induced by these cell lines in syngeneic rats showed reduction in the expression of both E-cadherin and á-catenin in the plasma membrane of invasive epithelial cells. Immunohistochemical analysis of NMBA-induced esophageal neoplasms in rats revealed E-cadherin and alpha-catenin to be abnormally expressed in poorly differentiated tumors when compared to well differentiated tumors. These results suggest that the microenvironment may have an important role in regulating the expression of these adhesion molecules in rat esophageal epithelial cells, and that alteration in the cellular localization of E-cadherin and alpha-catenin may be indicative of tumor progression in NMBA-induced rat esophageal cancer.
authors.affiliation: Department of Pathology, School of Public Health, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: L
authors.initials: L
authors.lastname: Khare
authors.forename: C L
authors.initials: CL
authors.lastname: Sabourin
authors.forename: B R
authors.initials: BR
authors.lastname: De Young
authors.forename: R J
authors.initials: RJ
authors.lastname: Jamasbi
authors.forename: G D
authors.initials: GD
authors.lastname: Stoner
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015820:Cadherins / Q000032:analysis*; D003598:Cytoskeletal Proteins / Q000032:analysis*; D004128:Dimethylnitrosamine / Q000031:analogs & derivatives / Q000633:toxicity; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured; D051178:alpha Catenin
chemical_list: D015820:Cadherins; D003598:Cytoskeletal Proteins; D051178:alpha Catenin; C014707:nitrosobenzylmethylamine; D004128:Dimethylnitrosamine