Isolation of rabbit liver heat shock protein with molecular weight 90 kD (Hsp90) and its interaction with troponin components and calponin.
Using a modified method consisting of chromatography on phenyl-Sepharose, Q-Sepharose, and hydroxyapatite, we isolated a highly purified heat shock protein with molecular weight 90 kD (Hsp90) from rabbit liver. The isolated protein was recognized on immunoblot by commercially available monoclonal anti-Hsp90 antibodies. The chromatographic properties, interaction with actin and calmodulin, phosphorylation in the presence of Mg-ATP, and one-dimensional peptide maps of rabbit liver Hsp90 are similar to the corresponding properties of Hsp90 isolated from other sources. In the presence of soluble carbodiimide and N-hydroxysuccinimide, rabbit liver Hsp90 can be cross-linked with calmodulin, troponin C, troponin I, and calponin. The data obtained indicate that Hsp90 may participate in the assembly of regulatory proteins of the actin filament.
Ma
Y S
YS
Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.
Bogatcheva
N V
NV
Gusev
N B
NB
D000199:Actins / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002845:Chromatography; D002852:Chromatography, Ion Exchange; D017886:Durapatite; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008840:Microfilament Proteins; D008970:Molecular Weight; D009124:Muscle Proteins / Q000737:chemistry / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D010449:Peptide Mapping; D011817:Rabbits; D014336:Troponin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D000096985:Calponins
D000199:Actins; D002135:Calcium-Binding Proteins; D017886:Durapatite; D018841:HSP90 Heat-Shock Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; D014336:Troponin; D000096985:Calponins