Transactivation by expression of the hepatitis B virus X protein with an inducible system.
We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
Azizan
A
A
Moffitt Cancer Center & Research Institute, Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa 33612, USA.
Cong
Y S
YS
Shain
K
K
Tsai
S C
SC
Yao
Y L
YL
Olashaw
N
N
Seto
E
E
D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D005347:Fibroblasts; D006515:Hepatitis B virus; D009687:Nuclear Proteins / Q000378:metabolism; D011233:Precipitin Tests; D011401:Promoter Regions, Genetic; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D013752:Tetracycline / Q000494:pharmacology; D015534:Trans-Activators / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D054334:Viral Regulatory and Accessory Proteins
D009687:Nuclear Proteins; D011500:Protein Synthesis Inhibitors; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein; D015500:Chloramphenicol O-Acetyltransferase; D013752:Tetracycline