Rapid hyaluronan uptake is associated with enhanced motility: implications for an intracellular mode of action.
Texas red-labeled hyaluronan (TR-HA) is rapidly taken up in a CD44 independent manner into ras-transformed 10T1/2 fibroblasts, where it accumulates in both cell ruffles/lamellae, the perinuclear area, and the nucleus. HA does not accumulate in the cell ruffles/lamellae of parental 10T1/2 cells. Addition of HA to ras-transformed cells promotes their random motility but has no effect on 10T1/2 cell motility. 10T1/2 cells can be modified to take up HA into cell ruffles by exposure to phorbol ester or direct microinjection of HA into cells. Both treatments significantly stimulate 10T1/2 cell motility.
Collis
L
L
The Hospital for Sick Children, Division of Cardiovascular Research, Toronto, Ont., Canada.
Hall
C
C
Lange
L
L
Ziebell
M
M
Prestwich
R
R
Turley
E A
EA
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001692:Biological Transport; D002461:Cell Line, Transformed; D002465:Cell Movement; D002467:Cell Nucleus / Q000378:metabolism; D011905:Genes, ras; D018960:Hyaluronan Receptors / Q000276:immunology; D006820:Hyaluronic Acid / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D010455:Peptides / Q000378:metabolism; D014170:Transformation, Genetic
D000906:Antibodies; D018960:Hyaluronan Receptors; D010455:Peptides; D006820:Hyaluronic Acid