title: Thermostable DNA-polymerase from Thermus thermophilus B35: isolation and characterization of some properties.
abstract: Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermophilus B35 which was found in hot spring water. The enzyme with molecular mass 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hydroxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol seems to be more favorable due to higher temperature optimum of this enzyme and lack of restriction endonucleases in the initial strain.
authors.affiliation: Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Rechkunova
authors.forename: A G
authors.initials: AG
authors.lastname: Akishev
authors.forename: N A
authors.initials: NA
authors.lastname: Lebedeva
authors.forename: S N
authors.initials: SN
authors.lastname: Khodyreva
authors.forename: S Kh
authors.initials: SKh
authors.lastname: Degtyarev
authors.forename: O I
authors.initials: OI
authors.lastname: Lavrik
mesh_terms: D002413:Cations, Divalent / Q000494:pharmacology; D002845:Chromatography; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D017886:Durapatite; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D013816:Thermodynamics; D016963:Thermus thermophilus / Q000201:enzymology*
chemical_list: D002413:Cations, Divalent; D017886:Durapatite; D004259:DNA-Directed DNA Polymerase