[Studies on synthetic peptide. XX: the antigenicity and linear epitope map of synthetic peptide hepatitis C virus].
Hepatitis C virus (HCV), the major causative agent of post transfusion non-A, non-B hepatitis (NANB), had been cloned and expressed. According to the protein sequence of HCV-BK and its epitope profiles which combined the hydrophilicity, accessibility, flexibility, antigenicity, charge distribution and HPLC reserve coefficient of protein using the "Goldkey" computer program, we designed and synthesized the following peptides: P1(475-495), P3(449-468), P4(658-663), P5(645-663), P6(484-489), P7(475-489), P15(655-662), P16(230-237), P17(225-237), P18(1220-1240), P19(1694-1735), P24(1230-1240), P25(1482-1493), P26(384-389), P27(2355-2389). The results of ELISA showed that P6(60% positive results) and P19(63% positive results) testing with PT-HC of Gu An, Hebei province were the major antigens in NS1 and in NS4 region, respectively.
Liu
G
G
School of Pharmaceutical Sciences, Beijing Medical University.
Liang
Z L
ZL
Cai
M S
MS
Zhuang
H
H
Guo
J P
JP
Tao
Q M
QM
D000956:Antigens, Viral / Q000276:immunology*; D000090266:Chromobox Protein Homolog 5; D000939:Epitopes; D016174:Hepacivirus / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D010452:Peptide Biosynthesis; D014761:Viral Hepatitis Vaccines / Q000138:chemical synthesis*; D014764:Viral Proteins / Q000138:chemical synthesis* / Q000276:immunology
D000956:Antigens, Viral; C000715594:CBX5 protein, human; D000939:Epitopes; D007106:Immune Sera; D014761:Viral Hepatitis Vaccines; D014764:Viral Proteins; D000090266:Chromobox Protein Homolog 5