title: Prechallenge high neutralizing antibodies and long-lasting immune reactivity to gp41 correlate with protection of rhesus monkeys against productive simian immunodeficiency virus infection or disease development.
abstract: To investigate the protective efficacy of various gp130 vaccine preparations, rhesus monkeys were immunized with gp130 oligomers (O-gp130) or two different gp130-monomer preparations (M1-gp130; M2-gp130) and challenged with 50 MID50 of simian immunodeficiency virus (SIV)mac32H. Following challenge the control animals and all animals of the M1- and M2-gp130 group and 1 animal of the O-gp130 group were productively infected, whereas 3 animals of the O-gp130 group resisted the productive virus replication. The protection was correlated with high neutralizing antibodies and a long-lasting immune response to the transmembrane protein gp41. Whereas none of the O-gp130 animals had developed disease symptoms, 3 M1-gp130 animals, 1 M2-gp130 animal, and 2 control animals died as a result of AIDS within 18 months after challenge. Therefore, immunization with virion-derived gp130 oligomers of SIVmac32H can confer protection against the productive infection with SIVmac32H and suppress the development of the AIDS-like disease.
authors.affiliation: Department of Virology and Immunology, German Primate Center, Göttingen.
authors.forename: H
authors.initials: H
authors.lastname: Petry
authors.forename: U
authors.initials: U
authors.lastname: Dittmer
authors.forename: D
authors.initials: D
authors.lastname: Jones
authors.forename: G
authors.initials: G
authors.lastname: Farrar
authors.forename: H
authors.initials: H
authors.lastname: Wachter
authors.forename: D
authors.initials: D
authors.lastname: Fuchs
authors.forename: T
authors.initials: T
authors.lastname: Nisslein
authors.forename: E
authors.initials: E
authors.lastname: Jurkiewicz
authors.forename: G
authors.initials: G
authors.lastname: Hunsmann
authors.forename: C
authors.initials: C
authors.lastname: Stahl-Hennig
authors.forename: W
authors.initials: W
authors.lastname: Lüke
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D015153:Blotting, Western; D004279:DNA, Viral / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000276:immunology; D019798:Neopterin / Q000652:urine; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D015531:Radioimmunoprecipitation Assay; D012191:Retroviridae Proteins / Q000276:immunology; D018449:SAIDS Vaccines; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D019562:Viral Load; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral; D015686:Gene Products, env; D007106:Immune Sera; D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; D018449:SAIDS Vaccines; C082614:SIV envelope protein gp41; C078684:simian immunodeficiency virus gp130; D019798:Neopterin