[Accumulation of a photosensitizer ATX-S 10 Na (II) to experimental corneal neovascularization].
In order to determine the most appropriate time point for laser irradiation in photodynamic therapy with a new photosensitizer, ATX-S 10 Na (II), which produces selective occlusion of new vessels, we investigated the time course of plasma levels of ATX-S 10 Na (II) after intravenous administration and degree of dye accumulation in the corneal neovascularization in rabbit eyes. Plasma ATX-S 10 Na (II) concentration decreased rapidly after injection and become virtually undetectable at 24 h, indicating rapid excretion from the body. Nitrogen-pulsed laser spectrofluorometry demonstrated that the amount of ATX-S 10 Na (II) in new corneal vessels increased and reached a maximal level 2 to 4 hours after dye injection. Under a fluorescence microscope, ATX-S 10 Na (II) was localized in the wall new corneal vessels and in extravascular tissue 2 to 4 hours after dye injection. These results indicate that the appropriate time for laser irradiation in selective PDT is between 2 and 4 hours after dye injection, when a larger amount of dye is accumulated in neovasculature tissue compared to normal tissue.
Gohto
Y
Y
Department of Ophthalmology, Osaka City University School of Medicine, Japan.
Obana
A
A
Kaneda
K
K
Nakajima
S
S
Takemura
T
T
Miki
T
T
D000818:Animals; D016510:Corneal Neovascularization / Q000188:drug therapy / Q000378:metabolism*; D053685:Laser Therapy; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000493:pharmacokinetics* / Q000627:therapeutic use; D011166:Porphyrins / Q000493:pharmacokinetics* / Q000627:therapeutic use; D011817:Rabbits; D013997:Time Factors
C117772:ATX S10Na(II); D017319:Photosensitizing Agents; D011166:Porphyrins