Host cell-dependent replication of HIV-1 gag MA, CA, and NC mutants are independent of the functions of Vif and Vpu.
We have previously shown that some gag gene mutants of human immunodeficiency virus type 1 (HIV-1) display a replication-defect in a cell-dependent manner. We and others have also demonstrated that the requirement of vif and vpu genes for HIV-1 replication is cell-dependent. To determine whether the cell-dependent growth of the HIV-1 gag mutants is related to the functions of Vif and Vpu, double mutants of gag-vif and gag-vpu were constructed, and monitored for their replication in various cell lines. The results obtained showed that the mutations in gag do not affect the cell-dependent functions of Vif and Vpu.
Shimano
R
R
Department of Virology, The University of Tokushima School of Medicine, Tokushima 770-8503, Japan.
Inubushi
R
R
Adachi
A
A
D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D015683:Gene Products, gag / Q000235:genetics*; D016346:Gene Products, vif / Q000235:genetics* / Q000502:physiology; D015488:HIV Antigens / Q000235:genetics; D016655:HIV Core Protein p24 / Q000235:genetics; D015497:HIV-1 / Q000235:genetics* / Q000472:pathogenicity; D054298:Human Immunodeficiency Virus Proteins; D006801:Humans; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000235:genetics; D014407:Tumor Cells, Cultured / Q000821:virology; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics* / Q000502:physiology; D014779:Virus Replication / Q000235:genetics* / Q000502:physiology; D054301:gag Gene Products, Human Immunodeficiency Virus; D054320:vif Gene Products, Human Immunodeficiency Virus; D000086082:Viroporin Proteins
D036022:Capsid Proteins; D015683:Gene Products, gag; D016346:Gene Products, vif; D015488:HIV Antigens; D016655:HIV Core Protein p24; D054298:Human Immunodeficiency Virus Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D054334:Viral Regulatory and Accessory Proteins; D054301:gag Gene Products, Human Immunodeficiency Virus; D054320:vif Gene Products, Human Immunodeficiency Virus; C071884:NCP7 protein, Human immunodeficiency virus 1; C054858:p17 protein, Human Immunodeficiency Virus Type 1; C514859:vpu protein, Human immunodeficiency virus 1; D000086082:Viroporin Proteins