An equation for the reconstitution of membrane proteins and a method for determination of pore content in biological membranes.
The dependence between specific trapped volume of liposomes (W) and protein concentration (p) is proposed to be used for quantitative pore determination in biological membranes via pore reconstitution into liposomes. This dependence is described by the following equation: p = -p(e) x ln(W/W0), where W0 is initial trapped volume of liposomes and pe is an equivalent protein concentration at which molar concentrations of pores and liposomes become equal. Experimentally determined equivalent protein concentration pe is the basis of the method. This method also permits determination of molar mass of pore-forming complexes provided that preparations contain purified complex.
Lagutina
L S
LS
Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071, Russia.
Sholtz
P K
PK
Mamaev
D V
DV
Sholtz
K F
KF
D000818:Animals; D003198:Computer Simulation; D007425:Intracellular Membranes / Q000378:metabolism; D007700:Kinetics; D008081:Liposomes / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D018272:Porins / Q000378:metabolism; D051381:Rats
D008081:Liposomes; D008565:Membrane Proteins; D018272:Porins