Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family.
The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.
Rodríguez
L
L
Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. yeastlab@cigb.edu.cu
Chávez
F P
FP
González
M E
ME
Basabe
L
L
Rivero
T
T
D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
D004271:DNA, Fungal; D005656:Fungal Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase