title: Nucleotide sequence of the putative replicase gene of the sour cherry strain of plum pox potyvirus.
abstract: The complete nucleotide sequence of the NIb coding region of the sour cherry strain of plum pox potyvirus (PPV-SoC) has been determined. It consists of 1554 nucleotides and encodes a putative replicase protein of 518 amino acids. Sequence identity scores between NIb of PPV-SoC and other isolates of PPV are significantly low (c. 78%). Many of the nucleotide substitutions, however, are silent. PPV-SoC differs from isolates of PPV-D, PPV-M and PPV-E1 Amar at multiple amino acid positions that are conserved between the other isolates. The NIb sequence extends the PPV-SoC sequence presently available to 2781 nt from the 3' end (approximately 28% of the genome).
authors.affiliation: Fruit Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland, USA.
authors.forename: L
authors.initials: L
authors.lastname: Nemchinov
authors.forename: J
authors.initials: J
authors.lastname: Hammond
authors.forename: A
authors.initials: A
authors.lastname: Hadidi
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D017801:Plum Pox Virus / Q000201:enzymology* / Q000235:genetics*; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
chemical_list: D012324:RNA-Dependent RNA Polymerase