title: Isolation of the receptor for Amaranthus leucocarpus lectin from murine peritoneal macrophages.
abstract: The receptor for Amaranthus leucocarpus lectin from CD-1 resident macrophages was purified with affinity chromatography with biotin labeled A. leucocarpus lectin and using avidin-agarose as affinity matrix. The receptor is a glycoprotein of 70 kDa that contains 18% of sugar by weight; it is mainly composed of galactose and N-acetyl-D-galactosamine in its saccharidic portion, and lacks sialic acid; the protein is rich in glycine, serine and alanine and lacks cysteine residues. The amino terminus of the receptor is blocked. By ionic strength chromatography on a mono P column in anionic form we purified three isoforms from the affinity purified receptor, each showing quantitative differences in glycosylation. The A. leucocarpus lectin receptor is identified only in resting, not activated, macrophages suggesting that it plays a role in activation mechanisms of macrophages.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, México.
authors.forename: P
authors.initials: P
authors.lastname: Gorocica
authors.forename: R
authors.initials: R
authors.lastname: Lascurain
authors.forename: P
authors.initials: P
authors.lastname: Hemández
authors.forename: F
authors.initials: F
authors.lastname: Porras
authors.forename: S
authors.initials: S
authors.lastname: Bouquelet
authors.forename: L
authors.initials: L
authors.lastname: Vázquez
authors.forename: E
authors.initials: E
authors.lastname: Zenteno
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000302:isolation & purification; D019921:Biotinylation; D002846:Chromatography, Affinity; D037502:Galectin 3; D037102:Lectins / Q000378:metabolism*; D017737:Macrophages, Peritoneal / Q000737:chemistry*; D008297:Male; D051379:Mice; D011485:Protein Binding; D020033:Protein Isoforms / Q000302:isolation & purification; D011975:Receptors, Mitogen / Q000302:isolation & purification*
chemical_list: D000943:Antigens, Differentiation; D037502:Galectin 3; D037102:Lectins; D020033:Protein Isoforms; D011975:Receptors, Mitogen