title: Diverse CD1d-restricted reactivity patterns of human T cells bearing "invariant" AV24BV11 TCR.
abstract: Human and murine natural T (NT) cells, also referred to as NK1.1+ or NK T cells, express TCR with homologous V regions (hAV24/BV11 and mAV14/BV8, respectively) and conserved "invariant" TCR AVAJ junctional sequences, suggesting recognition of closely related antigens. Murine NT cells recognize CD1-expressing cells and are activated in a CD1-restricted fashion by several synthetic alpha-glycosylceramides, such as alpha-GalCer. Here we studied the reactivity of human T cells against CD1d+ cells pulsed or not with alpha-GalCer and other related ceramides. CD1d-restricted recognition of alpha-GalCer was a general and specific feature of T cell clones expressing both BV11 and canonical AV24AJ18 TCR chains. Besides, human and murine NT cells showed the same reactivity patterns against a set of related glycosylceramides, suggesting a highly conserved mode of recognition of these antigens in humans and rodents. We also identified several AV24BV11 T cell clones self reactive against CD1+ cells of both hemopoietic and nonhemopoietic origin, suggesting the existence of distinct NT cell subsets differing by their ability to recognize self CD1d molecules.
authors.affiliation: INSERM U463, Institut de Biologie, Nantes, France.
authors.forename: C
authors.initials: C
authors.lastname: Couedel
authors.forename: M A
authors.initials: MA
authors.lastname: Peyrat
authors.forename: L
authors.initials: L
authors.lastname: Brossay
authors.forename: Y
authors.initials: Y
authors.lastname: Koezuka
authors.forename: S A
authors.initials: SA
authors.lastname: Porcelli
authors.forename: F
authors.initials: F
authors.lastname: Davodeau
authors.forename: M
authors.initials: M
authors.lastname: Bonneville
mesh_terms: D000818:Animals; D018949:Antigens, CD1 / Q000276:immunology*; D006801:Humans; D007135:Immunoglobulin Variable Region; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology*
chemical_list: D018949:Antigens, CD1; D007135:Immunoglobulin Variable Region; D011948:Receptors, Antigen, T-Cell