title: Interactions of a vesicular stomatitis virus G protein fragment with phosphatidylserine: NMR and fluorescence studies.
abstract: The interaction of a 19 amino acid vesicular stomatitis virus G protein fragment (GTWLNPGFPPQSCGYATVT) with phosphatidylserine-containing model membranes was investigated using solution-phase 1d and 2d 1H NMR spectroscopy and intrinsic tryptophan fluorescence. Results of these studies show that this peptide interacts with model membranes containing negatively charged phospholipids. The interaction is modulated by both ionic and hydrophobic factors and appears to be dependent on the fluidity and lipid packing of the target bilayer. The data further suggest the existence of two isomeric forms of this peptide, which react differentially with model membranes. Upon binding, 2d 1H NOESY and tryptophan fluorescence data indicate penetration of the tryptophan residue into the bilayer. A model is proposed for the interaction of the peptide with model membranes, consistent with the experimental findings.
authors.affiliation: Pharmaceutical Discovery Division, SRI International, Menlo Park, CA 94025, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Hall
authors.forename: K K
authors.initials: KK
authors.lastname: Burson
authors.forename: W H
authors.initials: WH
authors.lastname: Huestis
mesh_terms: D000595:Amino Acid Sequence; D009682:Magnetic Resonance Spectroscopy; D008562:Membrane Glycoproteins; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010718:Phosphatidylserines / Q000737:chemistry*; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D014721:Vesicular stomatitis Indiana virus / Q000737:chemistry; D014759:Viral Envelope Proteins / Q000737:chemistry*
chemical_list: C028391:G protein, vesicular stomatitis virus; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D010718:Phosphatidylserines; D014759:Viral Envelope Proteins