title: Successful transfer of ADA gene in vitro into human peripheral blood CD34+ cells by transfecting EBV-based episomal vectors.
abstract: We report a novel non-viral system for transfecting human immature hematopoietic cells in vitro. Epstein-Barr virus (EBV)-based episomal vectors carrying human adenosine deaminase (ADA) gene cDNA were transfected by electroporation into human peripheral blood (PB) CD34+ cells. The transgene-specific mRNA were detected from 37 to 100% of CFU-c (colony forming unit in culture) colonies derived from the transfected cells. A two-fold increase in enzyme activity was also found. These results indicate the successful transfer and expression of genes in human immature hematopoietic cells using the EBV-based episomal vector system.
authors.affiliation: Department of Microbiology, Kyoto Prefectural University of Medicine, Kamikyo, Japan.
authors.forename: E
authors.initials: E
authors.lastname: Satoh
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: C
authors.initials: C
authors.lastname: Shimazaki
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
authors.forename: J
authors.initials: J
authors.lastname: Imanishi
authors.forename: O
authors.initials: O
authors.lastname: Mazda
mesh_terms: D000243:Adenosine Deaminase / Q000096:biosynthesis / Q000235:genetics*; D015703:Antigens, CD / Q000097:blood; D018952:Antigens, CD34 / Q000097:blood*; D002478:Cells, Cultured; D017931:DNA Primers; D018274:Electroporation; D005822:Genetic Vectors; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use; D006412:Hematopoietic Stem Cells / Q000201:enzymology* / Q000473:pathology; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D009369:Neoplasms / Q000097:blood / Q000188:drug therapy; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000627:therapeutic use; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D015703:Antigens, CD; D018952:Antigens, CD34; D017931:DNA Primers; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor; D000243:Adenosine Deaminase