[Detection of p53 gene mutations in human gestational trophoblastic neoplasia by polymerase chain reaction-single strand conformational polymorphism techniques].
Using polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and EB staining technique, paraffin-embeded sections of 20 hydatidiform mole and 4 choriocarcinoma were detected in the exons 5 and 8 of p53 gene. The results showed that mutations of p53 gene were 0/9 in the normal chorionic villi, 6/20 (30%) in hydatidiform mole and 3/4 in choriocarcinoma. This study suggests that mutations of p53 gene may be an important event in human gestational trophoblastic neoplastigenesis and its progression.
Wu
Y
Y
Department of Gynecology and Obstetrics, Second Affiliated Hospital, Hunan Medical University, Changsha.
Zhang
Q
Q
Tang
H
H
D002822:Choriocarcinoma / Q000235:genetics*; D005260:Female; D016158:Genes, p53; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics*; D009154:Mutation; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000235:genetics*