Insertion mutagenesis of the ferric pyoverdine receptor FpvA of Pseudomonas aeruginosa: identification of permissive sites and a region important for ligand binding.
Insertion of an 18-amino-acid-encoding sequence within the fpvA gene identified permissive sites at residues Y350, A402, R451, R521, and R558, consistent with these residues occurring in extramembranous loop regions of the protein. Insertions at R451, R521, and R558 did not adversely affect receptor function, although insertions at Y350 and A402 compromised ferric pyoverdine binding and uptake. The latter region likely contributes to or interacts with the ligand-binding site.
Kilburn
L
L
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Poole
K
K
Meyer
J M
JM
Neshat
S
S
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D008024:Ligands; D016254:Mutagenesis, Insertional; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; C082326:FpvA protein, Pseudomonas aeruginosa; D008024:Ligands