Isolation and identification of inhibitory compounds on TNF-alpha production from Magnolia fargesii.
Three TNF alpha-inhibitory lignans were isolated from the flower buds of Magnolia fargesii through bioassay-guided isolation. They were identified as eudesmin, magnolin and lirioresinol-B dimethylether on the basis of their spectroscopic data. All three lignans showed inhibitory effects on TNF-alpha production in LPS-stimulated murine macrophage cell line, RAW264.7 and eudesmin showed the strongest activity (IC50 = 51 microM).
Chae
S H
SH
Research and Development Center, Daewoong Pharmaceutical Co., Sungnam, Korea.
Kim
P S
PS
Cho
J Y
JY
Park
J S
JS
Lee
J H
JH
Yoo
E S
ES
Baik
K U
KU
Lee
J S
JS
Park
M H
MH
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis*
D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha