The effect of extracellular matrix on the growth of mouse olfactory tissue in vitro.
The aim of this study was to compare the capacity of extracellular matrix (ECM) components to support the growth and differentiation of olfactory tissue in vitro. Reconstituted basement membrane matrix (RBM) supported profuse neurite outgrowth from explants of olfactory bulb (OB), with no non-neuronal cell migration. In contrast, nasal tissue (NT) explants cultured on RBM produced profuse fibroblastic outgrowth, but no neurite outgrowth from olfactory sensory neurones (OSN). Three-dimensional collagen gels, on the other hand, supported extensive outgrowth of both non-neuronal and neurite outgrowth from OB and NT explants, mature OSNs in the latter being identified using antibodies against olfactory marker protein (OMP). These culture systems represent useful tools for investigating potential tropic influences of target tissue.
Donnelly
G
G
Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, University of Glasgow, UK.
Smith
R A
RA
Breckenridge
L
L
D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002454:Cell Differentiation / Q000502:physiology; D002455:Cell Division / Q000502:physiology; D046508:Culture Techniques; D005109:Extracellular Matrix / Q000502:physiology*; D051379:Mice; D008808:Mice, Inbred CBA; D009830:Olfactory Bulb / Q000196:embryology*; D018034:Olfactory Receptor Neurons / Q000502:physiology