title: Identification of endothelial antigens relevant to transplant coronary artery disease from a human endothelial cell cDNA expression library.
abstract: Accelerated transplant coronary artery disease (TxCAD) results in increased expression of antiendothelial antibodies whose target antigens remain largely unidentified. One of these endothelial antigens has been identified as vimentin, a cytoskeletal protein present in cells of the blood vessel walls. In the present study, SDS-PAGE and Western blot analysis of human endothelial cell (EAHy 926) lysates probed with sera from a TxCAD patient were used to confirm immunoreactivity of antiendothelial antibodies towards several endothelial proteins. To further elucidate the identity of these putative antigens, a human endothelial cell (EAHy 926) cDNA expression library was immunoscreened with serum obtained from a TxCAD patient. Two positive cDNA clones were identified by partial nucleotide sequence analysis and GenBank/EMBL database searches for homology as the 85 kDa human CD36 antigen (a cell surface glycoprotein expressed in various cells including epithelial and endothelial cells) and a 50 kDa keratin-like protein (a member of the intermediate filament protein expressed in epithelial cells). These results are the first to demonstrate that human CD36 antigen and a keratin-like protein may be additional target proteins for the anti-endothelial antibodies associated with TxCAD.
authors.affiliation: Department of Haematological Medicine, Kings College School of Medicine and Dentistry, London SE5 9RS, UK.
authors.forename: A
authors.initials: A
authors.lastname: Ationu
mesh_terms: D000906:Antibodies / Q000097:blood; D000941:Antigens / Q000235:genetics*; D001483:Base Sequence; D002460:Cell Line; D003327:Coronary Disease / Q000097:blood / Q000276:immunology*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D015723:Gene Library; D016027:Heart Transplantation / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells; D008969:Molecular Sequence Data; D011183:Postoperative Complications / Q000276:immunology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014407:Tumor Cells, Cultured
chemical_list: D000906:Antibodies; D000941:Antigens; D018076:DNA, Complementary