title: An improved sample packing device for rapid freeze-trap electron paramagnetic resonance spectroscopy kinetic measurements.
abstract: A new method has been developed for sample packing in rapid freeze-quench electron paramagnetic resonance spectroscopy (EPR) kinetic experiments. Sample particles freeze-quenched in chilled isopentane are filtered under pressure through a stainless steel funnel attached to an EPR tube fitted with a porous disk at its bottom. Isopentane exits through the porous disk and the sample particles can be transferred essentially quantitatively into the receiving EPR tube. This device provides a more predictable, reproducible, and time-saving method for sample packing, enables use of a wider range of flow velocity, and allows efficient use of valuable reactants.
authors.affiliation: Department of Internal Medicine, University of Texas Medical School at Houston 77030, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: Tsai
authors.forename: V
authors.initials: V
authors.lastname: Berka
authors.forename: R J
authors.initials: RJ
authors.lastname: Kulmacz
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.forename: G
authors.initials: G
authors.lastname: Palmer
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D005374:Filtration; D005615:Freezing; D007700:Kinetics; D009211:Myoglobin / Q000378:metabolism; D010420:Pentanes; D011312:Pressure; D015203:Reproducibility of Results; D019810:Sodium Azide / Q000378:metabolism; D013048:Specimen Handling / Q000191:economics / Q000295:instrumentation* / Q000379:methods; D013232:Steel; D013594:Syringes; D013997:Time Factors
chemical_list: D009211:Myoglobin; D010420:Pentanes; D013232:Steel; D019810:Sodium Azide; C067038:isopentane