Determination of apramycin in swine kidney tissue by liquid chromatography with fluorescence detection.
A liquid chromatographic method for determining apramycin in swine kidney tissue is described. Apramycin is extracted from tissue with basic methanol and purified by ion-pair extraction. By using an automated derivatization and injection procedure, the purified extract is derivatized with o-phthaldehyde, separated on a C18 column, and detected with a fluorescence detector. For fortified kidney samples, between-run coefficients of variation ranged from 4.8 to 7.1% at 1.00 ppm and from 9.6 to 14.3% at 0.50 ppm. Recoveries ranged from 76 to 86%. Standard curves were linear over the range 10-100 ng/mL.
Sweeney
D J
DJ
Eli Lilly and Co., Greenfield, IN 46140, USA.
Coleman
M R
MR
D000085:Acetates; D064753:Ammonium Hydroxide; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005453:Fluorescence; D006878:Hydroxides; D007202:Indicators and Reagents; D007668:Kidney / Q000737:chemistry*; D013058:Mass Spectrometry; D009328:Nebramycin / Q000031:analogs & derivatives* / Q000032:analysis; D012972:Sodium Hydroxide; D013010:Sonication; D013552:Swine; D009764:o-Phthalaldehyde
D000085:Acetates; D006878:Hydroxides; D007202:Indicators and Reagents; D009328:Nebramycin; C011666:apramycin; D064753:Ammonium Hydroxide; D012972:Sodium Hydroxide; D009764:o-Phthalaldehyde; C007650:ethyl acetate