Reactivity of native conglutinin in bovine serum with rabbit antibody against recombinant bovine conglutinin with deletion of the N-terminal and collagen-like regions.
The reactivity of native bovine conglutinin (Kg) with antibody against recombinant Kg (rKg), with deletion of the N-terminal and collagen-like regions of the native Kg molecule, was studied by sandwich enzyme-linked immunosorbent assay. With anti-recombinant Kg antibody as the coating antibody, rKg reacted with biotinylated homologous anti-rKg and heterologous anti-Kg antibodies as probing antibodies, while native Kg did not. With anti-native Kg antibody as coating antibody, native Kg reacted with biotinylated homologous antibody as probing antibody, while recombinant Kg reacted weakly with both biotinylated homologous and heterologous antibodies. Consequently the N-terminal and collagen-like regions of native Kg molecule are essential to express the complete immunogenicity and/or antigenicity of the native Kg molecule.
Sugii
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S
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
Tajima
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D000818:Animals; D000937:Antigen-Antibody Reactions; D002417:Cattle / Q000276:immunology*; D003094:Collagen / Q000737:chemistry; D037222:Collectins; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D011994:Recombinant Proteins / Q000097:blood / Q000276:immunology; D017386:Sequence Homology, Amino Acid; D012712:Serum Globulins / Q000276:immunology*; D013054:Spectrophotometry, Atomic
D037222:Collectins; D010446:Peptide Fragments; D011994:Recombinant Proteins; D012712:Serum Globulins; C024014:conglutinin; D003094:Collagen