title: Longevity of lobsters is linked to ubiquitous telomerase expression.
abstract: Mammals have high growth rates in embryonic and juvenile phases and no growth in adult and senescent phases. We analyzed telomerase activity in a fundamentally different animal which grows indeterminately. Lobsters (Homarus americanus) grow throughout their life and the occurrence of senescence is slow. A modified TRAP assay was developed and the lobster telomeric repeat sequence TTAGG was determined. We detected telomerase activities which were dependent on RNA and protein components, required dGTP, dATP and dTTP, but not dCTP. Telomerase products with a five nucleotide periodicity were generated. High telomerase activities were detected in all lobster organs. We conclude that telomerase activation is a conserved mechanism for maintaining long-term cell proliferation capacity and preventing senescence, not only in cellular models or embryonic life stages but also in adult multicellular organisms.
authors.affiliation: Institute for Hematopathology, Center for Pathology and Applied Cancer Research, Christian-Albrechts-Universität Kiel, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Klapper
authors.forename: K
authors.initials: K
authors.lastname: Kühne
authors.forename: K K
authors.initials: KK
authors.lastname: Singh
authors.forename: K
authors.initials: K
authors.lastname: Heidorn
authors.forename: R
authors.initials: R
authors.lastname: Parwaresch
authors.forename: G
authors.initials: G
authors.lastname: Krupp
mesh_terms: D000818:Animals; D001483:Base Sequence; D015870:Gene Expression; D006801:Humans; D008136:Longevity / Q000235:genetics*; D008969:Molecular Sequence Data; D008121:Nephropidae / Q000201:enzymology; D019098:Telomerase / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014452:Ubiquitins / Q000737:chemistry
chemical_list: D014452:Ubiquitins; D019098:Telomerase