Optimal activation of isopsoralen to prevent amplicon carryover.
We compared the efficiencies of activation of the photochemical isopsoralen compound 10 and its resulting amplicon neutralizations under conditions with a UV transilluminator box at room temperature (RT) and a HRI-300 UV photothermal reaction chamber at RT and at 5 degrees C. Our data suggest that use of the HRI-300 reaction chamber at 5 degrees C results in a statistically significantly higher degree of amplicon neutralization.
Fahle
G A
GA
Microbiology Service, Clinical Pathology Department, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA. gfahle@nih.gov
Gill
V J
VJ
Fischer
S H
SH
D017931:DNA Primers / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D011564:Furocoumarins / Q000737:chemistry*; D010777:Photochemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D014466:Ultraviolet Rays
D017931:DNA Primers; D005456:Fluorescent Dyes; D011564:Furocoumarins; C011659:angelicin