A neurotrophic pyrimidine compound, MS-818, enhances EGF-induced restoration of gastric epithelial wounds in vitro.
MS-818 is a novel synthetic pyrimidine compound that stimulates nerve regeneration and promotes synthesis of various growth factors and differentiation of astrocytes. Effects of MS-818 on gastric epithelial cells were assessed using a wound repair model with primary cultured gastric epithelial cells from rabbits. A round wound with a constant cell-free area was created and the process of restoration was monitored by measuring wound size every 12 h. Cell proliferation was monitored by sequential staining with BrdU. As previously reported, EGF (10 ng/ml) accelerated wound repair by promoting cell migration and proliferation. Although MS-818 alone had no effects, MS-818 (10-100 microM) enhanced EGF-induced acceleration of gastric epithelial restoration, including cell migration and proliferation. Although the detailed mechanism of action of this agent is still unclear, MS-818 might have favorable effects on in vivo gastric mucosal repair.
Watanabe
S
S
Department of Gastroenterology, Juntendo University School of Medicine, Tokyo, Japan.
Wang
X E
XE
Hirose
M
M
Osada
T
T
Yoshizawa
T
T
Tanaka
H
H
Itatsu
T
T
Nakajima
M
M
Yamamoto
J
J
Miwa
H
H
Miyazaki
A
A
Awaya
A
A
Sato
N
N
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005753:Gastric Mucosa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology*; D011817:Rabbits; D013276:Stomach Ulcer / Q000473:pathology / Q000503:physiopathology*; D014945:Wound Healing / Q000187:drug effects*
C096499:MS 818; D018696:Neuroprotective Agents; D011743:Pyrimidines; D004815:Epidermal Growth Factor