title: Whole-body protein metabolism assessed by leucine and glutamine kinetics in adult patients with active celiac disease.
abstract: To assess the effect of increased renewal of intestinal epithelial cells on leucine and glutamine (Gln) turnover, 4-hour intravenous infusions of L-[1-(13)C]leucine and L-[2-(15)N]Gln were administered to five adult patients with active celiac disease in the postabsorptive state. There was a 35% increase in leucine flux (micromoles per kilogram per hour) in patients (117 +/- 17) compared with healthy controls (96 +/- 11, P < .03). Gln flux was increased by 13% in patients (377 +/- 35) versus controls (335 +/- 16, P < .04). These results suggest that active celiac disease, characterized by villous atrophy and crypt cell hyperplasia, is associated with a dramatic increase in whole-body protein breakdown as assessed by 13C-leucine, which may contribute per se to the protein malnutrition status of the patients. The increase in Gln utilization as assessed by L-[2-(15)N]Gln was moderate, but may have been offset due to the villose atrophy and ensuing reduced intestinal epithelial cell mass. The results are consistent with the concept that increased renewal of intestinal epithelial cells represents a sizable fraction of whole-body protein turnover and that Gln is an important fuel for epithelial intestinal cells in vivo.
authors.affiliation: Saint-Lazare Hospital, INSERM U290, Paris, France.
authors.forename: B
authors.initials: B
authors.lastname: Messing
authors.forename: S L
authors.initials: SL
authors.lastname: Dutra
authors.forename: F
authors.initials: F
authors.lastname: Thuillier
authors.forename: D
authors.initials: D
authors.lastname: Darmaun
authors.forename: J F
authors.initials: JF
authors.lastname: Desjeux
mesh_terms: D000328:Adult; D000368:Aged; D001835:Body Weight; D002446:Celiac Disease / Q000378:metabolism*; D005260:Female; D005973:Glutamine / Q000378:metabolism*; D006801:Humans; D007422:Intestines / Q000473:pathology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism*; D008875:Middle Aged; D012709:Serum Albumin / Q000378:metabolism
chemical_list: D012709:Serum Albumin; D005973:Glutamine; D007930:Leucine