title: A microfabricated device for sizing and sorting DNA molecules.
abstract: We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.
authors.affiliation: Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
authors.forename: H P
authors.initials: HP
authors.lastname: Chou
authors.forename: C
authors.initials: C
authors.lastname: Spence
authors.forename: A
authors.initials: A
authors.lastname: Scherer
authors.forename: S
authors.initials: S
authors.lastname: Quake
mesh_terms: D010582:Bacteriophage lambda; D004279:DNA, Viral / Q000302:isolation & purification*; D015247:Deoxyribonuclease HindIII; D008904:Miniaturization / Q000379:methods*; D008967:Molecular Biology / Q000295:instrumentation*
chemical_list: D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII