HPLC-fluorescence determination of unconjugated estrogens in pharmaceuticals.
A fluorimetric liquid chromatographic method (lambda(ex) = 280 nm; lambda(em) = 312 nm) was developed for measurements of unconjugated estrogens (estradiol and estriol) in pharmaceutical dosage forms using a reversed-phase column with water acetonitrile at different composition as mobile phase. The in vitro release profiles of three different estradiol transdermal therapeutic systems were determined through a medical-grade silicone rubber subdermal implant material membrane, using a modified Franz diffusion apparatus at 37 degrees C in presence of PEG 400. The HPLC method possesses advantages of rapidity, simplicity and accuracy.
Gatti
R
R
Dipartimento di Scienze Farmaceutiche, Bologna, Italy. rgatti@alma.unibo.it
Gioia
M G
MG
Di Pietra
A M
AM
Cavrini
V
V
D000279:Administration, Cutaneous; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000032:analysis* / Q000493:pharmacokinetics; D004964:Estriol / Q000032:analysis* / Q000493:pharmacokinetics; D009824:Ointments / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013607:Tablets / Q000378:metabolism
D009824:Ointments; D013607:Tablets; D004958:Estradiol; D004964:Estriol