The influence of phenobarbital on cytochromes and reactive oxygen species in erythropoietin producing HepG2 cells.
Light absorption photometry of HepG2 cells treated with phenobarbital for enhancing the content of cytochrome P-450 and the synthesis of erythropoietin revealed an influence on all cytochromes detectable in the wavelength range between 400 and 620 nm. No correlation was found between specific changes of cytochrome P-450 absorption and increased EPO synthesis as proposed earlier by Fandrey et al. (Life Sci. (1990) 47, 127-134). In the present study, however, the increased erythropoietin synthesis could be related to a decreased intracellular hydroxyl radical level described as crucial for the oxygen regulated gene expression (Kietzmann et al., Biochem. J. (1998) 335, 425-432; Porwol et al., Eur. J. Biochem. (1998) 256, 16-23).
Ehleben
W
W
Max Planck Institut für molekulare Physiologie, Dortmund, Germany.
Porwol
T
T
Fandrey
J
J
Acker
H
H
D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000187:drug effects* / Q000378:metabolism; D004921:Erythropoietin / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013057:Spectrum Analysis; D014407:Tumor Cells, Cultured
D003580:Cytochromes; D017382:Reactive Oxygen Species; D004921:Erythropoietin; D017665:Hydroxyl Radical; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital