Calcium binding protein calcyphosine in dog central astrocytes and ependymal cells and in peripheral neurons.
Calcyphosine is a calcium binding protein discovered in the dog thyroid in 1979. Calcyphosine mRNA and immunoreactivity were detected using Western and Northern blotting in the cerebral cortex, cerebral white matter and cerebellum. Using immunohistochemistry and in situ hybridization, both are present in ependymal cells, choroid plexus cells and several types of astrocytes of the subependymal cerebral layer, the cerebellar Bergmann layer, the retinal ganglion cell layer, the optic nerve and the posterior pituitary. Both are also present in neurons of nasal olfactory mucosa, enteric Auerbach and Meissner plexuses, orthosympathic and spinal cord ganglia as well as in endocrine cells of neural crest origin in the adrenal medulla. Calcyphosine immunoreactive astrocytes were also present mainly in hemispheric cerebral gray and white matter, hemispheric subcortical structures, brain stem and spinal cord. These results show that calcyphosine is a characteristic calcium binding protein of astrocytes and ependymal cells in the central nervous system and of neurons in the peripheral nervous system. This is of interest in view of the importance of calcium regulation in these cells, and since calcyphosine a calcium binding protein phosphorylated by cAMP dependent process, may be an intermediate between cAMP and inositol phosphate cascades.
Halleux
P
P
Laboratoire de Neuroanatomie, Neuropathologie et de Recherche sur les Peptides du Système Nerveux, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
Schurmans
S
S
Schiffman
S N
SN
Lecocq
R
R
Conreur
J L
JL
Dumont
J
J
Vanderhaeghen
J J
JJ
D000818:Animals; D001253:Astrocytes / Q000378:metabolism*; D015152:Blotting, Northern; D015153:Blotting, Western; D001921:Brain / Q000166:cytology; D001923:Brain Chemistry / Q000502:physiology; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D004285:Dogs; D004805:Ependyma / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D009474:Neurons / Q000378:metabolism*; D017933:Peripheral Nervous System / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism
C059605:CAPS protein, human; D002135:Calcium-Binding Proteins; D012333:RNA, Messenger