title: Identification and characterization of novel superantigens from Streptococcus pyogenes.
abstract: Three novel streptococcal superantigen genes (spe-g, spe-h, and spe-j) were identified from the Streptococcus pyogenes M1 genomic database at the University of Oklahoma. A fourth novel gene (smez-2) was isolated from the S. pyogenes strain 2035, based on sequence homology to the streptococcal mitogenic exotoxin z (smez) gene. SMEZ-2, SPE-G, and SPE-J are most closely related to SMEZ and streptococcal pyrogenic exotoxin (SPE)-C, whereas SPE-H is most similar to the staphylococcal toxins than to any other streptococcal toxin. Recombinant (r)SMEZ, rSMEZ-2, rSPE-G, and rSPE-H were mitogenic for human peripheral blood lymphocytes with half-maximal responses between 0.02 and 50 pg/ml (rSMEZ-2 and rSPE-H, respectively). SMEZ-2 is the most potent superantigen (SAg) discovered thus far. All toxins, except rSPE-G, were active on murine T cells, but with reduced potency. Binding to a human B-lymphoblastoid line was shown to be zinc dependent with high binding affinity of 15-65 nM. Evidence from modeled protein structures and competitive binding experiments suggest that high affinity binding of each toxin is to the major histocompatibility complex class II beta chain. Competition for binding between toxins was varied and revealed overlapping but discrete binding to subsets of class II molecules in the hierarchical order (SMEZ, SPE-C) > SMEZ-2 > SPE-H > SPE-G. The most common targets for the novel SAgs were human Vbeta2.1- and Vbeta4-expressing T cells. This might reflect a specific role for this subset of Vbetas in the immune defense of gram-positive bacteria.
authors.affiliation: Department of Molecular Medicine, School of Medicine, University of Auckland, 92019 Auckland, New Zealand.
authors.forename: T
authors.initials: T
authors.lastname: Proft
authors.forename: S L
authors.initials: SL
authors.lastname: Moffatt
authors.forename: C J
authors.initials: CJ
authors.lastname: Berkahn
authors.forename: J D
authors.initials: JD
authors.lastname: Fraser
mesh_terms: D000595:Amino Acid Sequence; D000942:Antigens, Bacterial / Q000276:immunology*; D001427:Bacterial Toxins / Q000235:genetics*; D001667:Binding, Competitive; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D005098:Exotoxins / Q000235:genetics*; D005798:Genes, Bacterial / Q000235:genetics; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D019169:Jurkat Cells; D008934:Mitogens / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013297:Streptococcus pyogenes / Q000276:immunology*; D018089:Superantigens / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D015032:Zinc / Q000378:metabolism
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D005098:Exotoxins; D000949:Histocompatibility Antigens Class II; D008934:Mitogens; D011994:Recombinant Proteins; D018089:Superantigens; C107894:mitogenic exotoxin Z protein, Streptococcus; D015032:Zinc