Microchimerism following allogeneic vascularized bone marrow transplantation--its possible role in induction of posttransplantation tolerance.
We have noticed that bone marrow transplanted in a vascularized limb graft providing a continuous supply of donor BMC may prolong the survival time of skin graft from the same donor. The question arises whether the raised microchimerism plays a role in the prolonged survival of skin allograft. The aim of the study was to follow the development of microchimerism after allogeneic vascularized bone marrow transplantation (VBMTx) concomitantly with the rejection processes of transplanted skin. The BN rats served as donors and LEW rats as recipients of VBMTx and free skin flap allograft. Hind limb was transplanted followed by a full-thickness skin graft on the dorsum. Cellular microchimerism was investigated in recipients of VBMTx and skin grafts in blood, spleen, mesenteric lymph node and bone marrow with monoclonal antibody OX27 directed against MHC class I polymorthic RTI on BN cells and quantitatively analysed in FACStar. In VBMTx group free skin flap survived 70 days after weaning of CsA. Intravenous infusion of BMC in suspension equivalent to that grafted in hind limb did not prolong skin graft survival after cessation of CsA therapy. Donor-derived cells could be detected in VBMTx recipients as long 70 days after wearing of CsA but not in recipients of i.v. suspension BMC grafting.
Durlik
M
M
Dept. of Surgical Research & Transplantology, Medical Research Center, Polish Academy of Sciences, Warsaw, Poland.
Lukomska
B
B
Ziółkowska
H
H
Namysłowski
A
A
Janczewska
S
S
Cybulska
E
E
Olszewski
W L
WL
D000818:Animals; D016026:Bone Marrow Transplantation / Q000276:immunology*; D006614:Hindlimb / Q000098:blood supply / Q000601:surgery / Q000637:transplantation*; D007165:Immunosuppression Therapy; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D016038:Skin Transplantation / Q000276:immunology*; D018183:Transplantation Chimera; D014184:Transplantation, Homologous / Q000276:immunology