The effects of serum from patients with acute liver failure on the growth and metabolism of Hep G2 cells.
In many bioartificial liver systems currently being designed and evaluated for use in fulminant hepatic failure, direct contact is required between the patient's blood and the liver cells in the device. The efficacy of such devices will be influenced by the interaction of fulminant hepatic failure (FHF) patient serum with the cells. We have found that FHF serum inhibits the growth rate and the synthesis of DNA, RNA, and protein; disturbs glutathione homeostasis; and induces morphological changes in cultured human Hep G2 cells. These interactions should influence the design of bioartificial liver devices based on proliferating cell lines and indicate the requirement to pretreat FHF patient plasma to reduce the toxin load.
Shi
Q
Q
Bioengineering Unit, University of Strathclyde, Wolfson Centre, Rottenrow, Glasgow, United Kingdom.
Gaylor
J D
JD
Cousins
R
R
Plevris
J
J
Hayes
P C
PC
Grant
M H
MH
D000818:Animals; D001790:Blood Physiological Phenomena; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology*; D017114:Liver Failure, Acute / Q000097:blood*; D008113:Liver Neoplasms; D019164:Liver, Artificial; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D014407:Tumor Cells, Cultured
D012313:RNA; D004247:DNA; D005982:Glutathione Transferase