Effects of extracellular matrix constituents on the attachment of human oral epithelial cells at the titanium surface.
This in vitro study attempts to delineate the role of extracellular matrix (ECM) constituents at the epithelial tissue-implant interface. To know which ECM constituents have a beneficial influence on the behavior of epithelial cells, the attachment, proliferation, morphologic pattern, and differentiation or cytoskeletal organization of human oral epithelial cells on ECM-coated (type IV collagen, fibronectin, type I collagen, laminin, and vitronectin) and noncoated titanium surface have been evaluated and compared. In each experiment comparing commercially pure titanium and oxygen plasma-cleaned titanium, the same ECM constituents were used. In this study, type IV collagen could provide an excellent substratum for epithelial cell attachment on titanium surface, but vitronectin-coated titanium revealed lower effectiveness for attachment of epithelial cells than noncoated titanium. These results suggested that type IV collagen could be used as a means for obtaining good epithelial seal, whereas vitronectin could be used to restrain the attachment of epithelium to dental implants.
Park
J C
JC
Department of Oral Histology, College of Dentistry, Chosun University, Kwang-ju, Korea.
Kim
H M
HM
Ko
J
J
D000328:Adult; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D015921:Dental Implants; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005069:Evaluation Studies as Topic; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D019096:Vitronectin / Q000494:pharmacology
D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019096:Vitronectin; D003094:Collagen; D014025:Titanium