Human D3 dopamine receptor in the medulloblastoma TE671 cell line: cross-talk between D1 and D3 receptors.
In search of a cell line in which the D3 dopamine receptor is expressed endogenously, we found that the neuron-derived human medulloblastoma cell line TE671 expresses the human D3 (hD3) and D1 (hD1) receptor, but neither the D2 or D4 receptors. Exposure of TE671 cells to the D3 agonist 7-OH-DPAT (DPAT), or to the D1 agonist SKF-38393 (SKF) increased the expression of hD3 or hD1 mRNA, respectively. Moreover, whereas DPAT had no effect on hD1 mRNA levels, stimulating the cells with SKF caused an increase in both hD1 and hD3 transcript levels. These results suggest (i) that following ligand stimulation, hD3 and hD1 receptors are upregulated to enhance their own receptor expression, and (ii) that upregulation of hD1 receptor transcripts leads to a stimulation of the hD3 dopamine receptor transcripts.
Levavi-Sivan
B
B
Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel.
Park
B H
BH
Fuchs
S
S
Fishburn
C S
CS
D000818:Animals; D002478:Cells, Cultured; D006801:Humans; D008527:Medulloblastoma; D051379:Mice; D011487:Protein Conformation; D017447:Receptors, Dopamine D1 / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D017448:Receptors, Dopamine D2 / Q000235:genetics* / Q000378:metabolism; D050637:Receptors, Dopamine D3; D015854:Up-Regulation
C493593:DRD3 protein, human; C493594:Drd3 protein, mouse; D017447:Receptors, Dopamine D1; D017448:Receptors, Dopamine D2; D050637:Receptors, Dopamine D3