The product of the mammalian orthologue of the Saccharomyces cerevisiae HBS1 gene is phylogenetically related to eukaryotic release factor 3 (eRF3) but does not carry eRF3-like activity.
We describe here the cloning and sequencing of human and mouse cDNAs encoding a putative GTP binding protein. Sequence comparison shows that these cDNAs (named eRFS) are likely to represent the orthologues of the yeast Saccharomyces cerevisiae HBS1 gene and that the C-terminal domains of the encoded proteins share structural features with eukaryotic elongation factor eEF-1A and release factor 3 (eRF3) families. The phylogenetic analysis suggests that eRFS proteins and Hbs1p form a cluster of orthologous sequences branching with the eRF3 family. Nevertheless, in yeast, the human eRFS protein and Hbs1p do not complement eRF3/Sup35p thermosensitive mutation and do not interact with eRF1.
Wallrapp
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C
Department of Internal Medicine I, University of Ulm, Germany.
Verrier
S B
SB
Zhouravleva
G
G
Philippe
H
H
Philippe
M
M
Gress
T M
TM
Jean-Jean
O
O
D000595:Amino Acid Sequence; D000818:Animals; D018388:Codon, Terminator; D018076:DNA, Complementary / Q000032:analysis; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010445:Peptide Elongation Factors; D010454:Peptide Termination Factors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
D018388:Codon, Terminator; D018076:DNA, Complementary; D005656:Fungal Proteins; C077247:HBS1 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; D010445:Peptide Elongation Factors; D010454:Peptide Termination Factors; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C087971:peptide-chain-release factor 3; D019204:GTP-Binding Proteins