A novel monoclonal antibody against rodent common Thy-1.1 epitope.
Here we report a new anti-rat Thy-1 monoclonal antibody (MAb) 12C5 that recognized a novel epitope on a GPI-anchored Thy-1 antigen. In rat thymocytes, MAb 12C5 reacted with 25 kDa Thy-1 antigen purified with MAb OX7. In rats, MAb 12C5 was expressed on 94% of thymocytes, 46% of spleen cells, 31% of mesenteric lymphnode cells, 76% of bone marrow cells, and 24% of splenic T cells. Furthermore, MAb 12C5 reacted with the hematopoietic cells in Mongolian gerbils, hamsters, and AKR mice (Thy-1.1) as in rats. In AKR mice, almost all bone marrow cells reacted with MAb 12C5. Thus, MAb 12C5 recognized a novel epitope of Thy-1 that was widespread in hematopoietic cells of rats and mice, as well as other rodents.
Kinebuchi
M
M
Department of Pathology, School of Medicine, Sapporo Medical University, Japan.
Matsuura
A
A
Uede
T
T
Ishii
Y
Y
Kikuchi
K
K
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001853:Bone Marrow / Q000276:immunology; D006224:Cricetinae; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology; D006825:Hybridomas / Q000276:immunology; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D015531:Radioimmunoprecipitation Assay; D051381:Rats; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D006023:Glycoproteins