title: Efficient gene transfer into normal human skeletal cells using recombinant adenovirus and conjugated adenovirus-DNA complexes.
abstract: In order to assess efficient DNA gene transfer into human primary cell cultures derived from the skeleton we tested two viral-based procedures. First, replication-deficient recombinant adenoviruses (ADV) were used to infect post-confluent human marrow stromal fibroblasts (HMSF) and human trabecular bone (HTB) cells. Both cell types were readily infected by modified adenoviral vectors carrying a reporter gene making this virus an attractive candidate to facilitate DNA gene transfer. In a second approach we coincubated DNA with ADV that had polylysine (PLL) covalently attached. With this ADV/PLL/DNA complex, very efficient gene transfer into multilayered HMSF and HTB cell cultures was observed, and DNA coincubated with unmodified ADV failed to be effectively transferred. These data imply that the covalently bound PLL more effectively binds exogenous DNA, resulting in a highly efficient internalization event in both cell types. Thus, this latter method has many advantages over conventional ADV gene transfer procedures. It is simple, rapid, and it does not require engineering of DNA into the viral genome, thereby allowing transfer of large fragments of DNA.
authors.affiliation: Craniofacial and Skeletal Diseases Branch, Building 30, Room 228, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: B
authors.initials: B
authors.lastname: Sommer
authors.forename: S A
authors.initials: SA
authors.lastname: Kuznetsov
authors.forename: P G
authors.initials: PG
authors.lastname: Robey
authors.forename: B
authors.initials: B
authors.lastname: O'Connell
authors.forename: R J
authors.initials: RJ
authors.lastname: Cristiano
authors.forename: M F
authors.initials: MF
authors.lastname: Young
mesh_terms: D000256:Adenoviridae; D001842:Bone and Bones / Q000502:physiology*; D002478:Cells, Cultured; D004247:DNA; D005347:Fibroblasts / Q000502:physiology; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors; D006801:Humans; D017154:Stromal Cells / Q000502:physiology
chemical_list: D004247:DNA