Antiserum against Cl- pump complex recognizes 51 kDa protein, a possible catalytic unit in the rat brain.
We purified Cl- pump in the rat brain and obtained 520 or 580 kDa protein complexes which consisted of 62, 60, 55 and 51 kDa proteins. An antiserum against 520 kDa protein complex recognized 51 kDa protein in both 520 and 580 kDa complexes, and reduced both Cl(-)-ATPase and Cl(-) pump activities. Such an immunoreactive 51 kDa protein was found in the brain, spinal cord and kidney. When incubated with [gamma-(32)P]ATP, the protein complex yielded phosphorylated 51 kDa protein, the label being hydroxylamine-sensitive and increased in the presence of Cl- and/or an inhibitor of Cl- pump, ethacrynic acid. Thus, the antibody appears to recognize a possible catalytic subunit of Cl- pump, 51 kDa protein, in the rat.
Zeng
X T
XT
Department of Pharmacology, Kansai Medical University, Moriguchi, Osaka, Japan.
Higashida
T
T
Hara
M
M
Hattori
N
N
Kitagawa
K
K
Omori
K
K
Inagaki
C
C
D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D027321:Anion Transport Proteins; D000918:Antibody Specificity / Q000502:physiology; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology; D002384:Catalysis; D002462:Cell Membrane / Q000201:enzymology; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000378:metabolism; D007668:Kidney / Q000201:enzymology; D008970:Molecular Weight; D009928:Organ Specificity; D010766:Phosphorylation; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology
D027321:Anion Transport Proteins; D007106:Immune Sera; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; C031652:anion-sensitive ATPases