Neutrophilia of infection/inflammation: are we really dealing with "inflamed" leukocytes?
We adopted whole blood flow cytometry and direct labeling of the CD11b/CD18 and CD62L antigens to study the relationship between their expression and leukocytosis in patients with infection/inflammation, acute stress and healthy volunteers. Mean +/- S.D. channel fluorescence intensity of CD11b/CD18 antigen on peripheral blood polymorphonuclears did not differ between patients with infection/ inflammation (173+/-78) and controls (167+/-72), but was significantly (p = 0.04) reduced in stress (135+/-60). No correlation was found between CD11b/CD18 antigen level and either polymorphonuclears absolute number or serum C-reactive protein. A significant negative correlation was noted between CD62L antigen expression on polymorphonuclears and their absolute number. We assume that cells with increased CD11b/CD18 surface concentrations are retained in the capillaries and that part of the leukocytes in the peripheral blood are stressed leukocytes with reduced CD11b/CD18. Thus, leukocytes detected in peripheral blood are not necessarily the most "inflamed" ones.
Sasson
Y
Y
Department of Internal Medicine D, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Israel.
Zeltser
D
D
Rogowski
O
O
Kassirer
M
M
Maharshak
N
N
Nissenkorn
A
A
Leibovitz
E
E
Halperin
P
P
Sharvit
D
D
Sorkine
P
P
Arber
N
N
Berliner
S
S
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018821:CD18 Antigens / Q000276:immunology; D003141:Communicable Diseases / Q000097:blood* / Q000276:immunology*; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007249:Inflammation / Q000097:blood* / Q000276:immunology*; D019041:L-Selectin / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D016177:Macrophage-1 Antigen / Q000276:immunology; D008297:Male; D008875:Middle Aged; D018375:Neutrophil Activation; D009504:Neutrophils / Q000276:immunology*
D018821:CD18 Antigens; D016177:Macrophage-1 Antigen; D019041:L-Selectin