title: Effects of extracellular matrix constituents on the attachment of human oral epithelial cells at the titanium surface.
abstract: This in vitro study attempts to delineate the role of extracellular matrix (ECM) constituents at the epithelial tissue-implant interface. To know which ECM constituents have a beneficial influence on the behavior of epithelial cells, the attachment, proliferation, morphologic pattern, and differentiation or cytoskeletal organization of human oral epithelial cells on ECM-coated (type IV collagen, fibronectin, type I collagen, laminin, and vitronectin) and noncoated titanium surface have been evaluated and compared. In each experiment comparing commercially pure titanium and oxygen plasma-cleaned titanium, the same ECM constituents were used. In this study, type IV collagen could provide an excellent substratum for epithelial cell attachment on titanium surface, but vitronectin-coated titanium revealed lower effectiveness for attachment of epithelial cells than noncoated titanium. These results suggested that type IV collagen could be used as a means for obtaining good epithelial seal, whereas vitronectin could be used to restrain the attachment of epithelium to dental implants.
authors.affiliation: Department of Oral Histology, College of Dentistry, Chosun University, Kwang-ju, Korea.
authors.forename: J C
authors.initials: JC
authors.lastname: Park
authors.forename: H M
authors.initials: HM
authors.lastname: Kim
authors.forename: J
authors.initials: J
authors.lastname: Ko
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D003094:Collagen / Q000494:pharmacology; D003599:Cytoskeleton / Q000187:drug effects; D015921:Dental Implants; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005069:Evaluation Studies as Topic; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D005353:Fibronectins / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D005881:Gingiva / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007797:Laminin / Q000494:pharmacology; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry; D019096:Vitronectin / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D019096:Vitronectin; D003094:Collagen; D014025:Titanium