Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
The first identification and characterization of a prokaryotic gene (spsA) encoding sucrose-phosphate synthase (SPS) is reported for Synechocystis sp. strain PCC 6803, a unicellular non-nitrogen-fixing cyanobacterium. Comparisons of the deduced amino acid sequence and some relevant biochemical properties of the enzyme with those of plant SPSs revealed important differences in the N-terminal and UDP-glucose binding site regions, substrate specificities, molecular masses, subunit compositions, and regulatory properties.
Curatti
L
L
Centro de Investigaciones Biológicas, Fundación para Investigaciones Biológicas Aplicadas (FIBA) and PROBIOP-CONICET, 7600 Mar del Plata, Argentina.
Folco
E
E
Desplats
P
P
Abratti
G
G
Limones
V
V
Herrera-Estrella
L
L
Salerno
G
G
D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D000458:Cyanobacteria / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli; D015870:Gene Expression; D005798:Genes, Bacterial; D005964:Glucosyltransferases / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016296:Mutagenesis; D016366:Open Reading Frames; D017421:Sequence Analysis
D005964:Glucosyltransferases; C022698:sucrose-phosphate synthase