In situ generated O-glycan core 1 structure as substrate for Gal(beta 1-3)GalNAc beta-1,6-GlcNAc transferase.
beta-Galactosidase from bovine testes was used in a one pot reaction together with a recombinant beta-1,6-GlcNAc transferase for the synthesis of GlcNAc(beta 1-6)GalNAc(alpha 1-OBn) (core 6-Bn). The galactosidase, which reversibly links galactose via a (beta 1-3) linkage to N-acetylgalactosamine, provides the substrate for the GlcNAc transferase in situ. The synthesis was carried out with a yield > 90%.
Dudziak
G
G
Forschungszentrum Jülich GmbH, Institut für Biotechnologie, Jülich, Germany.
Zeng
S
S
Berger
E G
EG
Gallego
R G
RG
Kamerling
J P
JP
Kragl
U
U
Wandrey
C
C
D000818:Animals; D002240:Carbohydrate Sequence; D002417:Cattle; D066298:In Vitro Techniques; D008297:Male; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D013737:Testis / Q000201:enzymology*; D014312:Trisaccharides / Q000378:metabolism*; D001616:beta-Galactosidase / Q000378:metabolism
D009844:Oligosaccharides; D011134:Polysaccharides; D000080506:Sialyl Lewis X Antigen; D014312:Trisaccharides; C073212:galactosyl beta(1-3)-N-acetylglucosaminyl-beta(1-6)-N-acetylgalactosamine; D017351:N-Acetylglucosaminyltransferases; C053024:beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-acetylglucosaminyl transferase; D001616:beta-Galactosidase