Passive particle agglutination test for screening of Treponema Pallidum antibodies in blood bank routine.
In most laboratories and blood banks, the TP.PA test has replaced the TPHA test for the screening of Treponema pallidum antibodies. In this study we show that the 1:20 serum dilution should be used to discriminate between negative and positive samples instead of the recommended 1:40 dilution, thus increasing sensitivity without a significant loss of specificity.
De Bleser
D
D
Laboratorium voor bacteriologie en virologie Universitair Ziekenhuis, De Pintelaan, Gent.
Van Renterghem
L
L
Vandekerckhove
B
B
D000907:Antibodies, Bacterial / Q000097:blood*; D001771:Blood Banks / Q000592:standards*; D001782:Blood Donors; D006679:HIV Seropositivity / Q000382:microbiology; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D008403:Mass Screening / Q000379:methods; D012680:Sensitivity and Specificity; D013587:Syphilis / Q000382:microbiology; D014210:Treponema pallidum / Q000276:immunology*
D000907:Antibodies, Bacterial