A combinatorial approach to chemical modification of subtilisin Bacillus lentus.
The reaction between methanethiosulfonate reagents and cysteine mutants of subtilisin is quantitative and can be used to prepare chemically modified mutant enzymes (CMMs) with novel properties. The virtually unrestricted structural variations possible for CMMs presents a preparative and screening challenge. To address this, a rapid combinatorial method for preparing and screening the activities of CMMs has been developed.
Plettner
E
E
Department of Chemistry, University of Toronto, Ontario, Canada.
Khumtaveeporn
K
K
Shang
X
X
Jones
J B
JB
D019943:Amino Acid Substitution; D001407:Bacillus / Q000201:enzymology*; D003545:Cysteine; D015195:Drug Design; D007202:Indicators and Reagents; D007700:Kinetics; D008698:Mesylates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D016297:Mutagenesis, Site-Directed; D017354:Point Mutation; D013329:Structure-Activity Relationship; D013381:Subtilisins / Q000737:chemistry* / Q000378:metabolism*
D007202:Indicators and Reagents; D008698:Mesylates; C074502:methanethiosulfonate; D013381:Subtilisins; D003545:Cysteine