title: Eliminating arterial pulsatile strain by external banding induces medial but not neointimal atrophy and apoptosis in the rabbit.
abstract: We have examined the role of vessel pulsation and wall tension on remodeling and intimal proliferation in the rabbit infrarenal abdominal aorta. A rigid perivascular polyethylene cuff was used to reduce vessel systolic diameter by 25%, producing a region of reduced circumferential strain. At 6 weeks postoperatively, reduced circumferential strain caused medial atrophy, with 45% reduction of medial area and 30% loss of medial smooth muscle cells. Apoptotic cell death was indicated by DNA fragmentation, propidium iodide staining, and cell morphology. Cuffing the aorta after balloon denudation produced medial atrophy but did not inhibit neointimal growth. At 1 week postoperatively, intimal thickness was slightly decreased in regions with reduced strain; however, intimal thickening in regions of reduced strain was not different from control segments at 3 weeks postoperatively (intimal area was 0.37 +/- 0.05 mm2 with reduced strain and 0.50 +/- 0.08 for controls, mean +/- SEM). We conclude that circumferential strain is a major factor controlling medial structure and cell number, whereas growth of the neointima after injury is not significantly affected by either reduced strain or extensive medial cell death. Vessel cuffing represents a new model of blood vessel remodeling in vivo that involves extensive smooth muscle cell apoptosis.
authors.affiliation: Terrence Donnelly Heart Centre, St. Michael's Hospital, and Department of Laboratory Medicine and Pathology, University of Toronto, Ontario, Canada. courtmand@aol.com
authors.forename: D W
authors.initials: DW
authors.lastname: Courtman
authors.forename: A
authors.initials: A
authors.lastname: Cho
authors.forename: L
authors.initials: L
authors.lastname: Langille
authors.forename: G J
authors.initials: GJ
authors.lastname: Wilson
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000473:pathology* / Q000648:ultrastructure; D017209:Apoptosis; D001284:Atrophy / Q000503:physiopathology*; D002404:Catheterization; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D011673:Pulsatile Flow / Q000502:physiology*; D011817:Rabbits; D013314:Stress, Mechanical; D017539:Tunica Intima / Q000473:pathology* / Q000648:ultrastructure; D017540:Tunica Media / Q000473:pathology* / Q000648:ultrastructure