Eight calves cloned from somatic cells of a single adult.
Eight calves were derived from differentiated cells of a single adult cow, five from cumulus cells and three from oviductal cells out of 10 embryos transferred to surrogate cows (80 percent success). All calves were visibly normal, but four died at or soon after birth from environmental causes, and postmortem analysis revealed no abnormality. These results show that bovine cumulus and oviductal epithelial cells of the adult have the genetic content to direct the development of newborn calves.
Kato
Y
Y
Laboratory of Animal Reproduction, College of Agriculture and Research Institute for Animal Developmental Biotechnology, Kinki University, 3327-204, Nakamachi, Nara, 631-8505, Japan.
Tani
T
T
Sotomaru
Y
Y
Kurokawa
K
K
Kato
J
J
Doguchi
H
H
Yasue
H
H
Tsunoda
Y
Y
D000822:Animal Husbandry; D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002417:Cattle / Q000196:embryology / Q000235:genetics*; D002459:Cell Fusion; D002478:Cells, Cultured; D019976:Cloning, Organism / Q000379:methods*; D003593:Cytoplasm / Q000502:physiology; D004624:Embryo Transfer / Q000662:veterinary; D004847:Epithelial Cells / Q000166:cytology; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D018895:Microsatellite Repeats; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000166:cytology*; D006080:Ovarian Follicle / Q000166:cytology*; D011247:Pregnancy; D011272:Pregnancy, Multiple