Synthesis of fluorescent substrates for protein tyrosine phosphatase assays.
Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O-phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.
Watanabe
T
T
Institute of Microbial Chemistry, Tokyo, Japan.
Imoto
M
M
Taniguchi
H
H
Kinoshita
K
K
Umezawa
K
K
D002462:Cell Membrane / Q000201:enzymology; D003619:Dansyl Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000737:chemistry* / Q000378:metabolism*; D019000:Phosphotyrosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D013449:Sulfonamides / Q000138:chemical synthesis* / Q000737:chemistry
D003619:Dansyl Compounds; D005456:Fluorescent Dyes; C117571:N-(5-(dimethylamino)naphthylsulfonyl)phosphotyrosine; C117572:N-(6-(dimethylamino)naphthylsulfonyl)phosphotyrosine; D013449:Sulfonamides; D019000:Phosphotyrosine; D017493:Leukocyte Common Antigens