title: Evaluation of increased aqueous outflow after radial keratotomy in enucleated bovine eyes.
abstract: OBJECTIVE
  We studied the efficacy of using radial keratotomy incisions as a surgical method of increasing outflow facility in enucleated bovine eyes.
  
  
  METHODS
  Each freshly enucleated bovine eye (n = 23) was cannulated to a system measuring the rate of flow into the eyes. Preoperatively, corneal thickness measurements were determined for all treated eyes. Radial keratotomy was performed in 13 eyes and a control set of eyes (n = 10) had no surgical manipulation. The intraocular pressure was set at 16.9 mm Hg. A clear zone of 10 mm was used for each eye with the incision depth set at 100% of the pachymetric reading. One set of experimental eyes had incisions that remained in the clear cornea, and the other set had incisions that reached 2 mm beyond the limbus into sclera. Preoperative outflow measurements were performed on each eye. Postoperative measurements were made after 10 incisions and 20 incisions. All eyes were checked for perforation after surgery and discarded if a perforation was found.
  
  
  RESULTS
  No eye included in the analysis was perforated. The preoperative (baseline) total flow rate was 27.90 +/- 3.72 mu 1/min at 16.9 mmHg. The flow rate increased by 23.5% (over baseline) with 20 radial keratotomy incisions. A paired t-test was used to statistically compare the outflow measurements before radial keratotomy and after the first and second set of radial incisions. The increases in flow were found to be statistically significant (P < .005) for both 10 and 20 radial incisions.
  
  
  CONCLUSIONS
  Assuming the flow measurements (flow rate) in these experiments parallel outflow facility, radial keratotomy increases the outflow facility in enucleated bovine eyes.
authors.affiliation: Department of Chemical Engineering, MIT, Boston, USA.
authors.forename: E P
authors.initials: EP
authors.lastname: Perez
authors.forename: S
authors.initials: S
authors.lastname: Weinreb
authors.forename: E
authors.initials: E
authors.lastname: Blanco
authors.forename: D
authors.initials: D
authors.lastname: Miller
mesh_terms: D000818:Animals; D001082:Aqueous Humor / Q000502:physiology*; D002417:Cattle; D003315:Cornea / Q000033:anatomy & histology / Q000601:surgery*; D015353:Eye Enucleation; D066298:In Vitro Techniques; D007429:Intraocular Pressure / Q000502:physiology*; D007646:Keratotomy, Radial; D011184:Postoperative Period