Modification of Ca2+, Mg2+-ATPase and F-actin distribution in hepatocytes of cyclosporine A treated rats. Effect of soyabean lecithin and triacylglycerol.
We studied the effect of cyclosporine A on hepatic Ca2+, Mg2+-ATPase and F-actin on bile canalicular and basolateral membranes in rats fed either soyabean lecithin, or triacylglycerol enriched diet, or low fat diet. Ca2+, Mg2+-ATPase histochemical activity was not modified in lecithin-cyclosporine A group, whereas the activity was decreased in the other groups. The triacylglycerol-cyclosporine A group had the lower activity. The histochemical staining of F-actin was quite normal in lecithin-cyclosporine group but decreased in the other cyclosporine A treated groups. The lower staining was observed in the triacylglycerol-cyclosporine group. The alteration of Ca2+, Mg2+-ATPase and F-actin by cyclosporine A, related to cholestasis evidenced by a decrease in bile salt secretion, were prevented by dietary soyabean lecithin and amplified by dietary soyabean triacylglycerol.
Benkoel
L
L
Laboratoire de Pathologie Cellulaire et Métabolique du Foie (JE 2058), Faculté de Médecine, Marseille, France.
Chanussot
F
F
Dodero
F
F
De La Maisonneuve
C
C
Lambert
R
R
Brisse
J
J
Delmas
M
M
Chamlian
A
A
D000199:Actins / Q000378:metabolism*; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D001648:Bile Canaliculi / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology*; D004041:Dietary Fats / Q000494:pharmacology; D008099:Liver / Q000378:metabolism; D008297:Male; D010713:Phosphatidylcholines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013025:Glycine max; D014280:Triglycerides / Q000494:pharmacology*
D000199:Actins; D001647:Bile Acids and Salts; D004041:Dietary Fats; D010713:Phosphatidylcholines; D014280:Triglycerides; D016572:Cyclosporine; D017301:Ca(2+) Mg(2+)-ATPase