[Retinol transport into the cell nucleus in vitro].
The retinol interaction with intact cellular nuclei, nuclear envelope and chromatin was investigated. We have shown that the Cellular Retinol-Binding Protein (CRBP) plays a very important role in such interaction. Retinol can specifically interacts with nuclei, nuclear envelope and chromatin only when it presents as a complex with CRBP. The obtained data allowed us to suggest that the process of retinol delivery to hypothetical nuclear receptors include of at least the following stages: 1) specific binding with nuclear envelope; 2) penetration into nuclei; 3) specific binding with chromatin receptors. CRBP is a necessary component at all this stages. Also we show that CRBP has not a species or tissue specificity.
Chetyrkin
S V
SV
A.V. Palladin Institute of Biochemistry, Kiev.
Chernukhina
L A
LA
Donchenko
G V
GV
D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D009685:Nuclear Envelope / Q000378:metabolism; D011485:Protein Binding; D012177:Retinol-Binding Proteins / Q000378:metabolism; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A / Q000378:metabolism*
D002843:Chromatin; D012177:Retinol-Binding Proteins; D054840:Retinol-Binding Proteins, Cellular; D014801:Vitamin A
Transport retinola v kletochnoe iadro in vitro.