title: The in vitro effect of leptin on basal and growth hormone-releasing hormone-stimulated growth hormone secretion from the ovine pituitary gland.
abstract: We have studied the direct effect of leptin on the secretion of GH from the anterior pituitary gland of the sheep. Using primary cultures of ovine pituitary cells, leptin (10(-9)-10(-7) M) treatment for 30 min did not affect basal or GHRH (10(-7) M)-stimulated GH secretion. Following treatment for 24 h, a dose of 10(-7) M leptin stimulated basal GH secretion. In contrast, doses of 10(-7) and 10(-8) M leptin inhibited GHRH-stimulated GH secretion after 24 h of treatment. These results suggest that leptin can have long-term effects on the somatotropes, but no acute effect. Furthermore, leptin appears to have opposite effects on basal and GHRH-stimulated GH secretion.
authors.affiliation: Prince Henry's Institute of Medical Research, Clayton, Vic., Australia.
authors.forename: S
authors.initials: S
authors.lastname: Roh
authors.forename: I J
authors.initials: IJ
authors.lastname: Clarke
authors.forename: R
authors.initials: R
authors.lastname: Xu
authors.forename: J W
authors.initials: JW
authors.lastname: Goding
authors.forename: K
authors.initials: K
authors.lastname: Loneragan
authors.forename: C
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D013006:Growth Hormone / Q000378:metabolism*; D013007:Growth Hormone-Releasing Hormone / Q000378:metabolism*; D020738:Leptin; D010902:Pituitary Gland / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D012756:Sheep; D013268:Stimulation, Chemical
chemical_list: D020738:Leptin; D011506:Proteins; D013006:Growth Hormone; D013007:Growth Hormone-Releasing Hormone