Systemic administration of epidermal growth factor reduces fat mass in rats: effects on the hormone-sensitive-lipase, lipoprotein lipase and leptin.
We examined whether the reduction in fat mass induced by EGF treatment in mature animals was via activation of hormone-sensitive lipase (HSL) and thereby the induction of lipolysis, or through inhibition of lipoprotein lipase activity thus reducing fat uptake in adipose tissue. Sixteen male rats were treated with placebo or EGF 150 microg/kg/day for 7 days via mini-osmotic pumps. The results demonstrate that systemic EGF treatment reduces the amount of adipose tissue, most likely due to increased lipolysis as HSL activity as well as HSL mRNA were increased. The circulating levels of free fatty acids were slightly increased and leptin levels reflected the decrease in adipose tissue mass.
Kristensen
K
K
Department of Endocrinology and Metabolism, Aarhus Amtssygehus and KH Aarhus University Hospital, Aarhus, Denmark. Kurt.Kristensen@aas.auh.dk
Pedersen
S B
SB
Vinter-Jensen
L
L
Flyvbjerg
A
A
Richelsen
B
B
D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000254:growth & development; D066246:ErbB Receptors / Q000096:biosynthesis / Q000235:genetics; D007328:Insulin / Q000097:blood; D020738:Leptin; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008297:Male; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D002787:Sterol Esterase / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
D001786:Blood Glucose; D007328:Insulin; D020738:Leptin; D011506:Proteins; D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D066246:ErbB Receptors; D002787:Sterol Esterase; D008071:Lipoprotein Lipase