title: Cryopreservation of Eisenia bicyclis (Laminariales, Phaeophyta) in liquid nitrogen.
abstract: Gametophytic cells of Eisenia bicyclis (Kjellman) Setchell were cryopreserved in liquid nitrogen (LN). Ethylene glycol was the most effective cryoprotectant when used alone, and in combination with 10% (w/v) proline improved the survival. Viability of female and male cryopreserved cells after thawing reached 62.0% and 52.6% immediately after thawing, but these levels decreased to 31.1% and 27.2% after 4 days postthawing culture. The optimal prefreezing temperature was -40 degreesC, and cells prefrozen to temperatures >-40 degreesC were damaged mainly by intracellular ice crystal formation during immersion in LN, while those prefrozen to temperatures <-40 degreesC were damaged mainly by excessive dehydration during prefreezing. The survival rates were not affected after storage in LN for at least 200 days.
authors.affiliation: Marine Greens Co., Ltd, 728 Mori, Iyo, Ehime 799-3125, Japan
authors.initials: S
authors.lastname: Kono
authors.initials: K
authors.lastname: Kuwano
authors.initials: N
authors.lastname: Saga