title: Mapping of 41 chemotaxis, flagellar and motility genes to a single region of the Sinorhizobium meliloti chromosome.
abstract: Three previously identified gene clusters that contain chemotaxis (che), flagellar (fla) and motility (mot) genes of Sinorhizobium meliloti (formerly Rhizobium meliloti) were mapped to a contiguous 45-kb region of the S. meliloti RU11/001 genome by pulsed-field gel electrophoresis (PFGE) in combination with Southern hybridization. The entire region was cloned and sequenced. The map combines 32 che, fla (flg, flh, fli) and mot genes and nine new open reading frames that probably encode taxis-related functions as well. It is concluded that between 80 and 90% of the genes responsible for chemotaxis and motility are located in a single region of the S. meliloti chromosome near the his-39 marker.
authors.affiliation: Lehrstuhl für Genetik, Universität Regensburg, D-93040, Regensburg, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Sourjik
authors.forename: W
authors.initials: W
authors.lastname: Sterr
authors.forename: J
authors.initials: J
authors.lastname: Platzer
authors.forename: I
authors.initials: I
authors.lastname: Bos
authors.forename: M
authors.initials: M
authors.lastname: Haslbeck
authors.forename: R
authors.initials: R
authors.lastname: Schmitt
mesh_terms: D002633:Chemotaxis / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005407:Flagella / Q000235:genetics*; D005798:Genes, Bacterial; D005810:Multigene Family; D020161:Physical Chromosome Mapping; D016962:Sinorhizobium meliloti / Q000235:genetics* / Q000502:physiology