High-resolution cardiac PET in rabbits: imaging and quantitation of myocardial blood flow.
A high-resolution PET system for small animals was tested for its applicability to the investigation of regional myocardial blood flow (MBF) in rabbits.


METHODS
Nineteen measurements were performed in 10 closed-chest anesthetized rabbits at baseline and during infusions of adenosine (0.2 mg/kg/min) and propranolol (0.20-1.20 mg slow infusion) to obtain a wide range of MBF. Myocardial blood flow was assessed both by dynamic 13N-ammonia PET and by colored microspheres. Blood was withdrawn directly from the femoral artery, and arterial 13N activity was measured by coincidence type gamma detection system for the input function. Nitrogen-13 myocardial uptake was calculated by dividing the myocardial 13N activity by the integral value of the input function.


RESULTS
Three or four contiguous cross-sectional myocardial images were obtained after 13N-ammonia injection. The left ventricular wall and cardiac cavity were clearly visualized. Moreover, initial passage of the tracer through the heart was obtained with serial 10-sec PET images. Nitrogen-13 myocardial uptake correlated well with flow measured with microspheres (r = 0.88).


CONCLUSIONS
Our cardiac PET system can be used for in vivo imaging and quantitation of MBF in small animals and may play an important role in the future study of animal models of cardiovascular diseases.
Shimada
K
K
National Institute of Radiological Sciences, and Third Department of Internal Medicine, Chiba University School of Medicine, Japan.
Yoshida
K
K
Tadokoro
H
H
Kitsukawa
S
S
Takami
A
A
Suzuki
K
K
Tanada
S
S
Masuda
Y
Y
D000241:Adenosine / Q000494:pharmacology; D000641:Ammonia / Q000493:pharmacokinetics; D000818:Animals; D003326:Coronary Circulation / Q000187:drug effects; D006321:Heart / Q000000981:diagnostic imaging* / Q000502:physiology; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism; D009590:Nitrogen Radioisotopes / Q000493:pharmacokinetics; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D012680:Sensitivity and Specificity; D013599:Systole / Q000187:drug effects; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed / Q000379:methods*
D009590:Nitrogen Radioisotopes; D000641:Ammonia; D011433:Propranolol; D000241:Adenosine