title: Role of mucosal IgA in the resistance to Acanthamoeba keratitis.
abstract: OBJECTIVE
  To determine whether oral immunization with Acanthamoeba castellanii antigens elicits mucosal antibodies of the IgA isotype and whether mucosal antibodies affect parasite adhesion to the corneal epithelium.
  
  
  METHODS
  Chinese hamsters were immunized with 100 microg aqueous Acanthamoeba antigen mixed with cholera toxin (Ac-CT) and subsequently challenged with parasite-laden contact lenses that were applied to abraded corneal surfaces. Tears and stool samples were examined for the presence of Acanthamoeba-specific IgA antibodies by enzyme-linked immunosorbent assay (ELISA). The effect of mucosal antibody on trophozoite binding to corneal epithelium and viability of trophozoites was examined in vitro.
  
  
  RESULTS
  Hamsters immunized orally with Ac-CT showed significantly lower infection rates than did control groups (21.4% versus 72.6%). ELISA analysis of mucosal specimens showed the presence of parasite-specific IgA in stool samples and tears from hamsters orally immunized with Ac-CT, but not in control animals. In vitro assays showed that anti-Acanthamoeba IgA did not affect parasite viability. However, mucosal anti-Acanthamoeba IgA profoundly inhibited (>75%) the binding of parasites to corneal epithelial cells in vitro.
  
  
  CONCLUSIONS
  Oral immunization with Ac-CT induces the production of parasite-specific IgA in mucosal secretions and prevents corneal infection. Mucosal antibody does not affect the viability of Acanthamoeba trophozoites but seems to prevent infection by inhibiting parasite binding to the corneal epithelium.
authors.affiliation: Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235, USA.
authors.forename: H F
authors.initials: HF
authors.lastname: Leher
authors.forename: H
authors.initials: H
authors.lastname: Alizadeh
authors.forename: W M
authors.initials: WM
authors.lastname: Taylor
authors.forename: A S
authors.initials: AS
authors.lastname: Shea
authors.forename: R S
authors.initials: RS
authors.lastname: Silvany
authors.forename: F
authors.initials: F
authors.lastname: Van Klink
authors.forename: M J
authors.initials: MJ
authors.lastname: Jager
authors.forename: J Y
authors.initials: JY
authors.lastname: Niederkorn
mesh_terms: D000048:Acanthamoeba / Q000276:immunology*; D015823:Acanthamoeba Keratitis / Q000276:immunology / Q000517:prevention & control*; D000284:Administration, Oral; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis / Q000502:physiology*; D000953:Antigens, Protozoan / Q000276:immunology; D003315:Cornea / Q000276:immunology / Q000469:parasitology; D006224:Cricetinae; D003412:Cricetulus; D003602:Cytotoxicity, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D018928:Immunity, Mucosal; D007114:Immunization; D007071:Immunoglobulin A, Secretory / Q000032:analysis / Q000502:physiology*; D009061:Mouth Mucosa / Q000276:immunology; D016052:Protozoan Vaccines / Q000008:administration & dosage*; D013666:Tears / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007071:Immunoglobulin A, Secretory; D016052:Protozoan Vaccines