title: Effect of captopril on intracellular pH in vascular smooth muscle cells.
abstract: OBJECTIVE
  To observe the effect of captopril (Cap) on intracellular pH (pHi) in aortic smooth muscle cells (ASMC).
  
  
  METHODS
  Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pHi was determined using digital image processing method.
  
  
  RESULTS
  The pHi of untreated SHR and WKY rats were 7.37 +/- 0.29 and 7.19 +/- 0.31, respectively. Oral Cap decreased pHi (7.11 +/- 0.26, P < 0.05) and exaggerated pHi response to angiotensin II (Ang-II, 0.1 mumol.L-1) in ASMC of SHR rats vs WKY rats (0.14 +/- 0.05 vs 0.21 +/- 0.05 pH units, P < 0.01). Cap in vitro had no effect on Ang-II induced intracellular alkalinization in ASMC of rabbits.
  
  
  CONCLUSIONS
  Oral Cap inhibits Na+(-)H+ exchange activity in ASMC of SHR rats.
authors.affiliation: Department of Pharmacology, Shanghai Second Medical University, China.
authors.forename: J H
authors.initials: JH
authors.lastname: Qi
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: P K
authors.initials: PK
authors.lastname: Gu
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Jin
authors.forename: M Z
authors.initials: MZ
authors.lastname: Huang
authors.forename: X M
authors.initials: XM
authors.lastname: Wang
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D001011:Aorta / Q000378:metabolism; D002216:Captopril / Q000494:pharmacology*; D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D002216:Captopril