Synthesis and evaluation of a photolyzable derivative of sphingosine 1-phosphate--caged SPP.
The synthesis of a photolyzable sphingosine 1-phosphate derivative is reported via the reaction of N-(tert-butoxycarbonyl)-2-N,3-O- isopropylidenesphingosine 7 and bis(alpha-methyl-o-nitrobenzyl) N,N-diisopropyl-phosphoramidite. Stimulation of DNA synthesis upon illumination of caged SPP-loaded cells is also described.
Qiao
L
L
Georgetown University Medical Center, Washington, DC 20007-2197, USA.
Kozikowski
A P
AP
Olivera
A
A
Spiegel
S
S
D016475:3T3 Cells; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000096:biosynthesis*; D007202:Indicators and Reagents; D008246:Lysophospholipids; D051379:Mice; D008958:Models, Molecular; D015394:Molecular Structure; D010755:Organophosphates / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D010782:Photolysis; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology
D007202:Indicators and Reagents; D008246:Lysophospholipids; D010755:Organophosphates; C116619:caged sphingosine 1-phosphate; C060506:sphingosine 1-phosphate; D004247:DNA; D013110:Sphingosine