Product activation of human erythrocyte AMP deaminase.
IMP was found to activate AMP deaminase in crude glucose-depleted human erythrocyte lysates. Activation of the enzyme by IMP is due to prevention of the inhibitory effect of inorganic phosphate. At 1 mM AMP and 2-3 mM phosphate the addition of 2-5 mM IMP accelerates the AMP deamination two to three times.
Mosharov
E V
EV
Research Center for Hematology, Russian Academy of Medical Sciences, Moscow. john@blood.ru
Vitvitsky
V M
VM
Ataullakhanov
F I
FI
D019794:2,3-Diphosphoglycerate / Q000494:pharmacology; D000659:AMP Deaminase / Q000037:antagonists & inhibitors / Q000097:blood*; D000249:Adenosine Monophosphate / Q000378:metabolism / Q000494:pharmacology; D002457:Cell Extracts; D003956:Dialysis; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D019271:Hypoxanthine / Q000494:pharmacology; D007288:Inosine / Q000494:pharmacology; D007291:Inosine Monophosphate / Q000494:pharmacology*; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology
D002457:Cell Extracts; D010710:Phosphates; D017680:Potassium Compounds; D007291:Inosine Monophosphate; D019794:2,3-Diphosphoglycerate; D019271:Hypoxanthine; D000249:Adenosine Monophosphate; D007288:Inosine; C013216:potassium phosphate; D000659:AMP Deaminase