Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.
Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.
Axelsson
M A
MA
Department of Medical Biochemistry, University of Göteborg, Gothenburg, Sweden.
Hansson
E M
EM
Sikut
R
R
Hansson
G C
GC
D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D005740:Gases; D006858:Hydrofluoric Acid; D008567:Membranes, Artificial; D018396:Mucin-1 / Q000737:chemistry / Q000276:immunology; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins / Q000737:chemistry* / Q000276:immunology; D009757:Nylons; D009844:Oligosaccharides
D000911:Antibodies, Monoclonal; D005740:Gases; D008567:Membranes, Artificial; D018396:Mucin-1; D055262:Mucin-2; D055263:Mucin-3; D009077:Mucins; D009757:Nylons; D009844:Oligosaccharides; D006858:Hydrofluoric Acid