title: Evidence for functional and physical association between Caenorhabditis elegans SEL-10, a Cdc4p-related protein, and SEL-12 presenilin.
abstract: Mutations in either of two human presenilin genes (PS1 and PS2) cause Alzheimer's disease. Here we describe genetic and physical interactions between Caenorhabditis elegans SEL-10, a member of the Cdc4p family of proteins, and SEL-12, a C. elegans presenilin. We show that loss of sel-10 activity can suppress the egg-laying defective phenotype associated with reducing sel-12 activity, and that SEL-10 can physically complex with SEL-12. Proteins of the Cdc4p family have been shown to target proteins for ubiquitin-mediated turnover. The functional and physical interaction between sel-10 and sel-12 therefore offers an approach to understanding how presenilin levels are normally regulated.
authors.affiliation: Department of Pathology and Center for Reproductive Sciences, Howard Hughes Medical Institute, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: G
authors.initials: G
authors.lastname: Wu
authors.forename: E J
authors.initials: EJ
authors.lastname: Hubbard
authors.forename: J K
authors.initials: JK
authors.lastname: Kitajewski
authors.forename: I
authors.initials: I
authors.lastname: Greenwald
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D002460:Cell Line; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; D005838:Genotype; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000502:physiology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014162:Transfection; D044767:Ubiquitin-Protein Ligases
chemical_list: D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D044783:F-Box Proteins; D000073979:F-Box-WD Repeat-Containing Protein 7; C513273:FBXW7 protein, human; D015801:Helminth Proteins; D008565:Membrane Proteins; D011994:Recombinant Proteins; C109554:SEL-10 protein, C elegans; C095907:SEL-12 protein, C elegans; D044767:Ubiquitin-Protein Ligases