[Studies on purging leukemic cells by photosensitizer PSD-007 laser photoradiation in vitro].
The sensitivity to photosensitization mediated by the hematoporphyrin photosensitizer PSD-007 of acute promyelocytic leukemic cell line (HL-60) was compared with normal human hemopoietic progenitor cells. The results showed that the leukemic cells were more sensitive. After being treated with 10 micrograms.ml-1 PSD-007 followed by 2J.cm-2 copper vapor laser light irradiation, the clonogenic leukemic (HL-60) cells were reduced 98%, but the survival rate of human granulocyte-macrophage colony-forming units (CFU-GM) was 40 +/- 8%. Mixing of normal human marrow cells with leukemic (HL-60) cells (ratio 100:1) did not interfere with elimination of tumor cells. The ultrastructure changes of HL-60 cells treated by laser photoradiation was observed under the trans-electronic microscope. The mitochondria, endoplasmic reticulum and cell membrane were involved. This means that the cell biomembrane is the main target to be attacked. It is considered that PSD-007-laser photoradiation therapy is efficient for killing leukemic cells.
Liang
X
X
Department of Hematology, Xiangya Hospital, Hunan Medical University, Changsha.
Qi
Z
Z
Cao
P
P
Xiao
J
J
Tang
G
G
Li
X
X
Lai
J
J
D000328:Adult; D016465:Bone Marrow Purging; D016026:Bone Marrow Transplantation; D018922:HL-60 Cells / Q000187:drug effects* / Q000648:ultrastructure; D006412:Hematopoietic Stem Cells / Q000187:drug effects; D017324:Hematoporphyrin Derivative / Q000494:pharmacology*; D006414:Hematoporphyrin Photoradiation; D006801:Humans; D017319:Photosensitizing Agents / Q000494:pharmacology*
D017319:Photosensitizing Agents; D017324:Hematoporphyrin Derivative