title: [3H]acetylcholine release from rat amacrine-like neurons is inhibited by adenosine A1 receptor activation.
abstract: We studied the effect of endogenous adenosine on the release of [3H]acetylcholine ([3H]ACh) in cultures enriched (96.4+/-0.4%) in rat cholinergic amacrine-like neurons, as determined by labeling with an antibody against choline acetyltransferase. A small population of these cells also contained GABA. Using these cultures we observed that both [3H]ACh release, which was largely Ca2+-dependent, and 45Ca2+ influx, evoked by depolarization with 50 mM KCl, were increased when adenosine A1 receptor activation was prevented by removal of endogenous adenosine with adenosine deaminase, or by application of the A1 receptor antagonist DPCPX. Our results indicate that, in cultured rat amacrine-like neurons, the activation of A1 receptors decreases calcium influx and, thereby, inhibits [3H]ACh release.
authors.affiliation: Center for Neuroscience of Coimbra, Department of Zoology, University of Coimbra, Portugal.
authors.forename: P F
authors.initials: PF
authors.lastname: Santos
authors.forename: O L
authors.initials: OL
authors.lastname: Caramelo
authors.forename: A P
authors.initials: AP
authors.lastname: Carvalho
authors.forename: C B
authors.initials: CB
authors.lastname: Duarte
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000243:Adenosine Deaminase / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels / Q000502:physiology; D002132:Calcium Radioisotopes / Q000493:pharmacokinetics; D002478:Cells, Cultured; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D004558:Electric Stimulation; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D018047:Receptors, Purinergic P1 / Q000378:metabolism*; D012160:Retina / Q000737:chemistry* / Q000166:cytology / Q000201:enzymology*; D014316:Tritium; D014970:Xanthines / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000032:analysis
chemical_list: D015220:Calcium Channels; D002132:Calcium Radioisotopes; D018047:Receptors, Purinergic P1; D014970:Xanthines; D014316:Tritium; D005680:gamma-Aminobutyric Acid; D011189:Potassium Chloride; C051360:1,3-dipropyl-8-cyclopentylxanthine; D002795:Choline O-Acetyltransferase; D000243:Adenosine Deaminase; D000109:Acetylcholine