title: Translocation of acylated pardaxin into cells.
abstract: Acylated pardaxin is translocated through the cytoplasmic membrane and is accumulated in the nucleoli of NG108-15 and chromaffin cells. The uptake is time- and dose-dependent and temperature-sensitive. However, the binding of acylated 125I-pardaxin cannot be reduced by competition with pardaxin acylated with Rudinger's reagent. In this respect, acylated pardaxin resembles the Tat protein 37-71 fragment. Metabolic inhibitors do not significantly reduce the uptake of acylated 125I-pardaxin. Acylated pardaxin might be useful as a vector to translocate other molecules.
authors.affiliation: Institute of Toxicology, Medical School of Hannover, Germany.
authors.forename: Y
authors.initials: Y
authors.lastname: Paul
authors.forename: A
authors.initials: A
authors.lastname: Weiss
authors.forename: K
authors.initials: K
authors.lastname: Adermann
authors.forename: G
authors.initials: G
authors.lastname: Erdmann
authors.forename: C
authors.initials: C
authors.lastname: Kassebaum
authors.forename: P
authors.initials: P
authors.lastname: Lazarovici
authors.forename: J
authors.initials: J
authors.lastname: Hochman
authors.forename: H
authors.initials: H
authors.lastname: Wellhöner
mesh_terms: D000215:Acylation; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001692:Biological Transport; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002463:Cell Membrane Permeability; D002466:Cell Nucleolus / Q000378:metabolism*; D019439:Chromaffin Cells / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005397:Fish Venoms / Q000378:metabolism* / Q000494:pharmacology; D005452:Fluoresceins / Q000378:metabolism; D018613:Microscopy, Confocal; D013696:Temperature; D013997:Time Factors
chemical_list: D005397:Fish Venoms; D005452:Fluoresceins; C017603:pardaxin; C043829:2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein