Comparison of an anti-HSV-2 monoclonal IgG and its IgA switch variant for topical immunoprotection of the mouse vagina.
An IgG2a monoclonal antibody (Mab) directed against glycoprotein D of herpes simplex virus 2 (HSV-2) was compared with an IgA heavy chain Mab switch variant to investigate the effect of isotype for topical immunoprotection of the murine vagina. The IgA Mab, a mixture of monomeric and polymeric IgA, was indistinguishable from its IgG parent in an in vitro HSV-2 neutralization assay. When these class switched Mabs were delivered to the mouse vagina, we also found no significant difference between the IgG and IgA for preventing vaginal transmission of HSV-2 infection. The implications of these results for active and passive immunization strategies against vaginal transmission of genital herpes infections are discussed.
Zeitlin
L
L
Department of Biophysics, The Johns Hopkins University, Baltimore, MD 21218, USA.
Castle
P E
PE
Whaley
K J
KJ
Moench
T R
TR
Cone
R A
RA
D000287:Administration, Topical; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000914:Antibodies, Viral / Q000276:immunology*; D000940:Antigenic Variation / Q000276:immunology; D005260:Female; D006558:Herpes Genitalis / Q000517:prevention & control*; D018258:Herpesvirus 2, Human / Q000276:immunology*; D006801:Humans; D007116:Immunization, Passive; D007070:Immunoglobulin A / Q000276:immunology*; D017578:Immunoglobulin Class Switching / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D014621:Vagina
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G