title: Analysis of the evolutionarily conserved repeat motifs in the genome of the highly endangered central Indian swamp deer Cervus duvauceli branderi.
abstract: We have analyzed the genome of central Indian swamp deer Cervus duvauceli branderi, an inhabitant of the Kanha National Park, a wildlife conservatory in Central India, with a view to provide a genetic basis for their extinction. Evolutionarily conserved repeat sequence motifs (GATA)3.75, TA(GATA)4, (GACA)3.75, (TGG)6 and a set of mouse beta-actin primers were used to uncover the sequence variation within and between related species by employing techniques of hybridization and AP-PCR amplification. The oligo probe carrying the GACA and TGG repeat motifs was found to be positive with Cervus genome, whereas (GATA)3.75, TA(GATA)4 and beta-actin probes did not cross-hybridize with the same. AP-PCR amplification with (GACA)3.75, unlike the (TGG)6 primer, generated distinct bands in the range of 0. 37-2.10kb amongst different genomes including Cervus. A comparative genome analysis of other species using the AP-PCR approach with (GACA)3.75 primer revealed the phylogenetic status of Cervus duvauceli branderi. From the analysis of a very limited number of Cervus DNA samples, we observed a high level of genetic homogeneity that may be a prime reason for the extinction of this species. This study has implications in the context of conservation of this endangered Cervus duvauceli branderi species.
authors.affiliation: Molecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110 067, India.
authors.forename: S
authors.initials: S
authors.lastname: Ali
authors.forename: S
authors.initials: S
authors.lastname: Ansari
authors.forename: N Z
authors.initials: NZ
authors.lastname: Ehtesham
authors.forename: M A
authors.initials: MA
authors.lastname: Azfer
authors.forename: U
authors.initials: U
authors.lastname: Homkar
authors.forename: R
authors.initials: R
authors.lastname: Gopal
authors.forename: S E
authors.initials: SE
authors.lastname: Hasnain
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D003670:Deer / Q000235:genetics*; D019143:Evolution, Molecular; D005260:Female; D014644:Genetic Variation; D016678:Genome; D006720:Homozygote; D007194:India; D051379:Mice; D018895:Microsatellite Repeats; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D013045:Species Specificity
chemical_list: D000199:Actins