Nuclear localization of prostaglandin E2 receptors.
Prostaglandin E2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP1 and in human embryonic kidney 293 (Epstein-Barr virus-encoded nuclear antigen) cells expressing EP1 fused to green fluorescent protein. High-resolution immunostaining of EP1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.
Bhattacharya
M
M
Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada H3G 1Y6.
Peri
K G
KG
Almazan
G
G
Ribeiro-da-Silva
A
A
Shichi
H
H
Durocher
Y
Y
Abramovitz
M
M
Hou
X
X
Varma
D R
DR
Chemtob
S
S
D016475:3T3 Cells; D000818:Animals; D000831:Animals, Newborn; D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002560:Cerebrovascular Circulation; D015232:Dinoprostone / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000378:metabolism*; D019309:Epstein-Barr Virus Nuclear Antigens / Q000235:genetics / Q000378:metabolism; D049452:Green Fluorescent Proteins; D006801:Humans; D007668:Kidney; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008833:Microcirculation; D008854:Microscopy, Electron; D018078:Receptors, Prostaglandin E / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013552:Swine
D019309:Epstein-Barr Virus Nuclear Antigens; D008164:Luminescent Proteins; D018078:Receptors, Prostaglandin E; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D015232:Dinoprostone