In vivo linearization and autonomous replication of plasmids containing human telomeric DNA in Aspergillus nidulans.
Plasmids containing two inverted 0.6-kb stretches of human telomeric repeats transform Aspergillus nidulans at frequencies characteristic of autonomously replicating vectors. Transformation frequency is not affected when the plasmids are linearized in vitro prior to transformation by cutting between the inverted repeats. Southern analysis reveals the presence of a homogeneous pool of linear plasmid molecules in mycelium of transformants. Addition of the AMA1 plasmid replicator to the telomere-containing plasmids has only a minor effect on transformation. The phenotypic stability of the transformants is low. However, unlike conventional replicative transformants containing AMA1-bearing plasmids, these transformants are prone to spontaneous stabilization which occurs predominantly by conversion of the mutant chromosomal allele of the marker gene to the plasmid-borne allele. The data strongly suggest that telomeric DNA can act as a plasmid replicator. An alternative interpretation is that autonomous replication of linear DNA fragments, in contrast to covalently closed supercoiled molecules, does not require any special replicator sequences.
Aleksenko
A
A
Center for Process Biotechnology, Department of Biotechnology Technical University of Denmark (DTU), Lyngby. aal@ibt.dtu.dk
Ivanova
L
L
D001233:Aspergillus nidulans / Q000235:genetics*; D015139:Blotting, Southern; D004261:DNA Replication; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors; D006801:Humans; D008938:Mitosis; D009154:Mutation; D010957:Plasmids / Q000235:genetics*; D016615:Telomere / Q000235:genetics*; D014170:Transformation, Genetic