title: Intraspecies polymorphism of vsp genes and expression profiles of variable surface protein antigens (Vsps) in field isolates of Mycoplasma bovis.
abstract: To assess the extent of interstrain variation, 50 isolates of Mycoplasma (M.) bovis including the type strain PG45 were examined for the presence of a family of variable membrane surface lipoproteins (Vsps) and their genes. Southern hybridization using a genomic fragment carrying three distinct vsp genes (vspAEF) revealed a striking heterogeneity, with only 2/50 strains having identical banding patterns. Cluster analysis of the data showed that most isolates from interrelated herds (groups 1, 2 and 3) were combined in a cluster of 50% homology, while isolates from distinct geographical regions (groups 4, 5 and 6) were linked only at 18% homology. Vsp antigen expression was monitored by Western immunoblotting using four specific monoclonal antibodies (MAbs). Resembling the findings at the DNA level, interstrain variation of Vsp expression among groups 1-3 was less pronounced than among non-interrelated isolates from groups 4-6. Ten out of 50 strains did not hybridize with the vspAEF gene probe at high-stringency conditions, 8/50 failed to react with any of the Vsp-related MAbs, and 6/50 proved negative in both assays. Interestingly, most of these isolates produced hybridization signals at low stringency suggesting major distinctions in their vsp gene structure. The extensive evidence obtained on interstrain vsp gene polymorphism and variation in Vsp expression could provide a basis for a future understanding of the pathogenic potential of individual M. bovis strains.
authors.affiliation: Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Beier
authors.forename: H
authors.initials: H
authors.lastname: Hotzel
authors.forename: I
authors.initials: I
authors.lastname: Lysnyansky
authors.forename: C
authors.initials: C
authors.lastname: Grajetzki
authors.forename: M
authors.initials: M
authors.lastname: Heller
authors.forename: B
authors.initials: B
authors.lastname: Rabeling
authors.forename: D
authors.initials: D
authors.lastname: Yogev
authors.forename: K
authors.initials: K
authors.lastname: Sachse
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D005260:Female; D005798:Genes, Bacterial; D008074:Lipoproteins / Q000096:biosynthesis / Q000235:genetics; D008414:Mastitis, Bovine / Q000382:microbiology; D008969:Molecular Sequence Data; D009174:Mycoplasma / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000382:microbiology / Q000662:veterinary*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D008074:Lipoproteins