title: Correlation of testicular pathology and sperm extraction in azoospermic men with ejaculated spermatids detected by immunofluorescent localization.
abstract: Limiting testicular biopsy for intracytoplasmic sperm injection (ICSI) to those with a high chance of having testicular spermatozoa has not been possible because of the poor predictive value of current clinical and laboratory methods. In order to predict testicular pathology and sperm extraction, we characterised the semen of 28 men with azoospermia due to gonadal failure in terms of the presence of spermatids using an immunological method. The results were compared with the assessment of testicular biopsies by histology and the extraction of spermatozoa into culture medium. Washed cellular elements in the ejaculate were smeared on microscope slides and fixed in 100% methanol, before incubation with acrosome-specific monoclonal antibody (18.6), fluorescein isothiocyanate-labelled anti-mouse goat IgG, and examination by epifluorescent microscopy. Semen from men with oligozoospermia and obstructive azoospermia served as positive and negative controls, respectively. Twelve patients who had positive immunofluorescence (one or more spermatids present) had spermatozoa retrieved from their testes (five hypospermatogenesis, seven focal spermatogenesis), and 16 patients with negative immunofluorescence (spermatids absent) had apparent Sertoli cell-only syndrome (12) or maturation arrest histological pattern (four). However, four patients with apparent Sertoli cell-only syndrome had testicular spermatozoa present after extraction from the biopsy. Plasma follicle stimulating hormone concentration and testicular volume did not predict retrieval of seminal spermatids or testicular spermatozoa. We conclude that the immunofluorescent localization of one or more spermatids in the ejaculate can be used to predict the likelihood of obtaining testicular spermatozoa for ICSI. However, in some patients with Sertoli cell-only syndrome, spermatozoa could still be recovered in the absence of apparent seminal spermatids.
authors.affiliation: University Department of Obstetrics and Gynaecology, Jessop Hospital For Women, Sheffield, UK.
authors.forename: U I
authors.initials: UI
authors.lastname: Ezeh
authors.forename: M
authors.initials: M
authors.lastname: Martin
authors.forename: I D
authors.initials: ID
authors.lastname: Cooke
authors.forename: H D
authors.initials: HD
authors.lastname: Moore
mesh_terms: D000177:Acrosome / Q000276:immunology; D000911:Antibodies, Monoclonal; D001706:Biopsy; D005455:Fluorescent Antibody Technique; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D008297:Male; D009845:Oligospermia / Q000473:pathology*; D012661:Semen / Q000166:cytology; D013048:Specimen Handling / Q000379:methods; D013081:Sperm Motility; D013087:Spermatids / Q000473:pathology*; D013091:Spermatogenesis; D013094:Spermatozoa / Q000473:pathology*; D013737:Testis / Q000473:pathology*
chemical_list: D000911:Antibodies, Monoclonal; D005640:Follicle Stimulating Hormone