Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant.
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.
Kim
J R
JR
Department of Biochemistry, College of Medicine, Yeungnam University, Daegu, Korea.
Kim
S Y
SY
Kim
J H
JH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D017931:DNA Primers; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006023:Glycoproteins / Q000235:genetics*; D007939:Leukemia L1210 / Q000188:drug therapy / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis
D017931:DNA Primers; D006023:Glycoproteins; C116582:arr protein, mouse; D004317:Doxorubicin