Effect of adrenalectomy on the slimming activity of liposome-carried oleoyl-estrone in the rat.
OBJECTIVE
To determine the extent of glucocorticoid counter-regulatory control in the slimming action of oleoylestrone.


METHODS
Control and adrenalectomized rats were subjected to a seven-day treatment with 3.5 micromol/kg/d oleoylestrone in liposomes injected i.v. continuously by implanted osmotic minipumps.


METHODS
Sham-operated control and adrenalectomized lean Zucker rats.


METHODS
Body weight and food intake; plasma glucose, urea, insulin, leptin and corticosterone; liver glycogen.


RESULTS
Treatment with oleoyl-estrone resulted in decreases in body weight and in food intake, as well as in circulating glucose, insulin and leptin. Combined adrenalectomy and oleoyl-estrone treatment resulted in a loss of almost 15% body weight in only seven days, with a severe drop in circulating glucose and insulin, almost total disappearance of plasma leptin and liver glycogen and a 3-fold rise in circulating urea. Food intake decreased sharply, which resulted in the exhaustion of energy reserves.


CONCLUSIONS
The results presented here, strongly support the hypothesis that glucocorticoids play an important role in the modulation of oleoyl-estrone-induced imbalance of energy intake and expenditure. The large effect of oleoyl-estrone on glucose, glycogen- and protein-derived (urea levels) energy in adrenalectomized rats, provides more evidence for the assumed protective role of glucocorticoids against the oleoyl-estrone-induced net loss of energy reserves. The results also show the powerful destabilizing effects of unchecked oleoyl-estrone on energy balance.
Grasa
M M
MM
Centre d'Investigació Laboratoris SALVAT, SA, Esplugues deLlobregat Barcelona, Spain.
Cabot
C
C
Adán
C
C
Vilà
R
R
Esteve
M
M
Estruch
J
J
Fernández-López
J A
JA
Remesar
X
X
Alemany
M
M
D000315:Adrenalectomy; D000818:Animals; D019440:Anti-Obesity Agents; D003345:Corticosterone / Q000097:blood; D004435:Eating; D002149:Energy Intake; D004734:Energy Metabolism; D004970:Estrone / Q000008:administration & dosage / Q000031:analogs & derivatives*; D005260:Female; D007328:Insulin / Q000097:blood; D020738:Leptin; D008081:Liposomes; D009829:Oleic Acids / Q000008:administration & dosage*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011924:Rats, Zucker; D015431:Weight Loss
D019440:Anti-Obesity Agents; D007328:Insulin; D020738:Leptin; D008081:Liposomes; D009829:Oleic Acids; D011506:Proteins; D004970:Estrone; C108709:oleoyl-estrone; D003345:Corticosterone