A microfabricated device for sizing and sorting DNA molecules.
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.
Chou
H P
HP
Department of Applied Physics, California Institute of Technology, Pasadena, CA 91125, USA.
Spence
C
C
Scherer
A
A
Quake
S
S
D010582:Bacteriophage lambda; D004279:DNA, Viral / Q000302:isolation & purification*; D015247:Deoxyribonuclease HindIII; D008904:Miniaturization / Q000379:methods*; D008967:Molecular Biology / Q000295:instrumentation*
D004279:DNA, Viral; D015247:Deoxyribonuclease HindIII