Mammalian coronary perfusion--an improved laboratory device.
An improvised device for coronary perfusion of isolated rat heart consisted of a reservoir of buffer pressurised with continuous bubbling of O2 connected to two double condensors in series. The outer jacket of each condenser was connected to a temperature controlled motorised water bath to ensure steady temperature of buffer. Isolated hearts could be tested with known agents directly through a syringe connected to the aortic cannula.
Nayeem
M
M
Department of Physiology & Biochemistry, Madras Veterinary College, Chennai, India.
D000818:Animals; D002021:Buffers; D006321:Heart / Q000502:physiology*; D066298:In Vitro Techniques; D010100:Oxygen; D010477:Perfusion / Q000295:instrumentation*; D051381:Rats; D013696:Temperature
D002021:Buffers; D010100:Oxygen