title: Detection of bovine milk proteins in soymilk by Western blotting.
abstract: A Western blotting method for the detection of whey milk proteins in commercial soymilks was applied to assess the food safety. Soy proteins and milk proteins were separated by SDS-PAGE in PhastSystem equipment. After the electrophoretic separation, immunodetection with anti-bovine alpha-lactalbumin and anti-bovine beta-lactoglobulin antisera was performed. Adulteration with bovine protein in percentages of 0.1% in soy protein can be detected. Western blotting of bovine alpha-lactalbumin and bovine beta-lactoglobulin was applied to detect adulteration by bovine milk proteins in different soymilks: powdered soymilk and soy infant formulas.
authors.affiliation: Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Molina
authors.forename: L
authors.initials: L
authors.lastname: Amigo
authors.forename: M
authors.initials: M
authors.lastname: Ramos
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005506:Food Contamination / Q000517:prevention & control; D005513:Food Inspection / Q000379:methods* / Q000592:standards; D007225:Infant Food / Q000032:analysis*; D008894:Milk Proteins / Q000032:analysis*; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max / Q000378:metabolism*
chemical_list: D008894:Milk Proteins; D030262:Soybean Proteins