A new model to assess staphylococcal adhesion to intraocular lenses under in vitro flow conditions.
Adhesion of staphylococcal cells to intraocular lenses coated with heparin was studied under in vitro flow conditions (280 microl min(-1)) at 37 degrees C. The intraocular lenses were incubated with human cerebrospinal fluid for 1 h or with cerebrospinal fluid including 0.50% plasma for 12 h, prior to bacterial challenge. Two strains of Staphylococcus epidermidis selected for this study, were isolated from biomaterial-associated infections. Bacterial adhesion was quantitated by bioluminescence and visualized by fluorescence microscopy of acridine orange stained bacteria. Surface coating with heparin significantly decreased bacterial adhesion of both strains after incubation with cerebrospinal fluid including 0.50% plasma for 12 h (p = 0.0209). However, no difference in bacterial adhesion was obtained between intraocular lenses with and without heparin, after incubation with cerebrospinal fluid for 1 h (p = 0.327). Microscopy showed that more bacteria were present on intraocular lenses without heparin than on intraocular lenses with heparin. The results show that preincubation with a proteinaceous fluid influences subsequent bacterial adhesion to the polymer surface. The results suggest that IOLs with heparin coating may be less prone to bacterial adhesion under perfusion conditions after surface conditioning in human CSF with 0.50% plasma and a preincubation period of 12 h. Heparin coating might be a valuable tool to decrease implant-associated bacterial endophthalmitis.
Lundberg
F
F
Department of Medical Microbiology, Lund University, Sweden.
Gouda
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I
Larm
O
O
Galin
M A
MA
Ljungh
A
A
D000165:Acridine Orange; D001422:Bacterial Adhesion; D005353:Fibronectins / Q000097:blood / Q000134:cerebrospinal fluid; D006493:Heparin; D006801:Humans; D007910:Lenses, Intraocular / Q000009:adverse effects*; D008163:Luminescent Measurements; D008853:Microscopy; D008856:Microscopy, Fluorescence; D013212:Staphylococcus epidermidis; D019096:Vitronectin / Q000097:blood / Q000134:cerebrospinal fluid
D005353:Fibronectins; D019096:Vitronectin; D006493:Heparin; D000165:Acridine Orange