title: Determination of aloesin in plasma by high-performance liquid chromatography as fluorescent 9-anthroyl derivative.
abstract: A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and detected fluorimetrically at 460 nm with excitation at 360 nm. The detection limit of aloesin was 3.2 ng/ml in plasma (S/N = 3).
authors.affiliation: College of Pharmacy, Kangwon National University, Chunchon, Korea.
authors.forename: K H
authors.initials: KH
authors.lastname: Kim
authors.forename: J G
authors.initials: JG
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Park
authors.forename: Y G
authors.initials: YG
authors.lastname: Shin
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: T H
authors.initials: TH
authors.lastname: Cho
authors.forename: S T
authors.initials: ST
authors.lastname: Oh
mesh_terms: D000873:Anthracenes / Q000032:analysis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis / Q000097:blood*; D005456:Fluorescent Dyes; D005960:Glucosides / Q000032:analysis / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D000873:Anthracenes; D002867:Chromones; D005456:Fluorescent Dyes; D005960:Glucosides; C066291:9-anthroylnitrile; C069868:aloesin