Production of amylase by Aspergillus fumigatus utilizing alpha-methyl-D-glycoside, a synthetic analogue of maltose, as substrate.
A strain of Aspergillus fumigatus isolated from soil was able to produce biomass and high amylase activities in media containing alpha-methyl-D-glucoside (alphaMG), a synthetic analogue of maltose, as the only carbon source. alphaMG was a more effective inducer than starch and maltose at the same concentration: alphaMG cultures produced about 3 times more alpha-amylase and glucoamylase activity than starch cultures. Maximum production of alpha-amylase (60 U/mg) and glucoamylase (130 U/mg) was obtained in 8-10 days alphaMG cultures.
Goto
C E
CE
Departamento de Bioquímica, Universidade Estadual de Maringá, Brazil.
Barbosa
E P
EP
Kistner
L C
LC
Moreira
F G
FG
Lenartovicz
V
V
Peralta
R M
RM
D000681:Amylases / Q000096:biosynthesis*; D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000254:growth & development; D003470:Culture Media; D004790:Enzyme Induction; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism*
D003470:Culture Media; D008757:Methylglucosides; C027020:methylglucoside; D000681:Amylases; D005087:Glucan 1,4-alpha-Glucosidase