title: [A juxtaglomerular cell tumor. Analysis of immunohistochemistry, electron microscopy and in situ hybridization].
abstract: We present a case of juxtaglomerular cell tumor measuring 8 mm in diameter in the right kidney. The hypertension was cured and plasma renin activity returned to normal level following tumor resection with partial nephrectomy. We studied histopathologic, electron microscopic, and immunohistochemical findings of the tumor. The majority of tumor cells stores renin granules in cytoplasm. In situ hybridization confirmed us that the most tumor cells produce renin. We use the digoxigenin labeled 0.6-kb length RNA probe of human renal renin, the specificity was analyzed by competition assay (1:50).
authors.affiliation: Department of Urology, Tokyo Women's Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Hashimoto
authors.forename: H
authors.initials: H
authors.lastname: Nakazawa
authors.forename: H
authors.initials: H
authors.lastname: Okuda
authors.forename: F
authors.initials: F
authors.lastname: Ito
authors.forename: S
authors.initials: S
authors.lastname: Onizuka
authors.forename: K
authors.initials: K
authors.lastname: Kihara
authors.forename: M
authors.initials: M
authors.lastname: Suzuki
authors.forename: Y
authors.initials: Y
authors.lastname: Maeda
authors.forename: H
authors.initials: H
authors.lastname: Toma
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure; D000328:Adult; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000601:surgery / Q000648:ultrastructure; D008297:Male; D009392:Nephrectomy; D015347:RNA Probes; D012333:RNA, Messenger / Q000032:analysis; D012083:Renin / Q000032:analysis*
chemical_list: D015347:RNA Probes; D012333:RNA, Messenger; D012083:Renin