Hydrogen peroxide determination in pharmaceutical formulations and cosmetics using a new catalase biosensor.
The possibility of evaluating the content of hydrogen peroxide in several authentic matrices, such as cosmetic and pharmaceutical formulations, was studied. A new catalase biosensor fabricated using an amperometric gas-diffusion oxygen sensor as electrochemical transducer and the catalase enzyme immobilized in kappa-carrageenan gel and capable of operating in both aqueous and non aqueous solvents was developed and tested for this purpose. Creams, emulsions and disinfectant solutions were analysed. To this end, a preliminary check was needed to establish the best conditions to analyse these matrices; the choice of solvent was one of the most important points studied. The solvents considered included dioxane, water-dioxane mixtures, water saturated chloroform and aqueous solutions. The different solubility properties of the matrices analysed were taken into account.
Campanella
L
L
Department of Chemistry University of Rome La Sapienza, Italy. campanella@axrma.uniroma.l.it
Roversi
R
R
Sammartino
M P
MP
Tomassetti
M
M
D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D002374:Catalase / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003358:Cosmetics / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000032:analysis*; D017186:Titrimetry / Q000379:methods
D003358:Cosmetics; D006861:Hydrogen Peroxide; D002374:Catalase