The influence of carbon source on the level and composition of ceramides of the Candida lipolytica yeast.
Candida lipolytica yeast was grown batchwise on two different carbon sources, glucose and n-hexadecane. Free ceramides were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative thin-layer chromatography. Their composition, after acid methanolysis, was analysed by gas-liquid chromatography. The ceramide content accounted for 2.6% of the total cell lipids in hexadecane-grown cells, which was 1.5 times higher than in glucose-grown cells. The fatty acid composition of ceramides was characterized by the predominance of fatty acids shorter than 20 carbon atoms and by high concentrations of fatty acids with 16 carbon atoms after growth on both carbon sources. The dominant fatty acid was hydroxylated 16:0 in the glucose-grown cells and 16:0 in the hexadecane-grown cells. The striking finding was the low degree of fatty acid hydroxylation and relatively high proportion of odd-numbered fatty acids in ceramide of the n-hexadecane-grown cells. The ceramides contained an unusual long-chain base composition. In hexadecane-grown cells more than 60% of the long-chain bases were C19 phytosphingosine. In glucose-grown cells more than one-half of the total long-chain bases were tetrahydroxy bases, 4,5-dihydroxysphinganine and 4,5-dihydroxyeicosasphinganine.
Rupcić
J
J
Department of Chemistry and Biochemistry, Medical Faculty, University of Rijeka, Croatia. rjasmin@mamed.medri.hr
Mesarić
M
M
Marić
V
V
D000473:Alkanes / Q000378:metabolism*; D002175:Candida / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D002518:Ceramides / Q000032:analysis* / Q000378:metabolism; D002849:Chromatography, Gas; D003470:Culture Media; D005227:Fatty Acids / Q000032:analysis; D005947:Glucose / Q000378:metabolism*; D008055:Lipids / Q000032:analysis; D013110:Sphingosine / Q000031:analogs & derivatives / Q000032:analysis
D000473:Alkanes; D002518:Ceramides; D003470:Culture Media; D005227:Fatty Acids; D008055:Lipids; C007932:n-hexadecane; C012491:phytosphingosine; D005947:Glucose; D013110:Sphingosine