A new series of cyclic amino acids as inhibitors of S-adenosyl L-methionine synthetase.
Optically active 3-amino-3-(tetrahydrofuranyl) carboxylic acid, 3-amino-3-(tetrahydrothienyl) carboxylic acid and their corresponding six membered ring analogues have been synthesised and examined as potential inhibitors of the enzyme S-adenosylmethionine (AdoMet) synthetase. The kinetic behaviour of these compounds was studied using recombinant rat liver AdoMet synthetase (alpha-isoform) fractionated from E. coli transformed with the plasmid pSSRL-T7N. All the compounds tested were competitive inhibitors of the enzyme with respect to L-methionine.
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Laboratoire de chimie bioorganique, U.M.R. 6519, U.F.R. Sciences de Reims, France.
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D000598:Amino Acids, Cyclic / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008716:Methionine Adenosyltransferase / Q000037:antagonists & inhibitors*; D051381:Rats; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors
D000598:Amino Acids, Cyclic; D004791:Enzyme Inhibitors; D011994:Recombinant Proteins; D008716:Methionine Adenosyltransferase