[Trypsin inhibitors of wheat seedlings infected and treated with salicylic acid].
Using the method of affinity chromatography for trypsin-sepharose, followed by PAGE electrophoresis the component composition of trypsin inhibitors was studied. The inhibitors were extracted from the winter wheat seedlings which were infected with Fusarium fungus and also from the seedlings treated with salicylic acid. Under the action of pathogen not only a quantitative increase in the protein components of trypsin inhibitor but also the appearance of a new component with a molecular weight of 29 kDa are observed. The influence of Fusarium graminearum and salicylic acid on the amino acid composition of trypsin inhibitors has been studied. After the inhibitors purification their antienzyme spectrum was studied along with their influence on the pathogen proteases. It was established that the inhibitors influence only the activity of trypsin and chymotrypsin and do not influence the activity of pepsin, elastases, papain, proteinases from the wheat seedlings. The existence of arginine and absence of lysine was demonstrated in the active centre of the inhibitors.
Molodchenkova
O O
OO
Breeding and Genetic Institute, Odessa.
Levitskiĭ
A P
AP
Levitskiĭ
Iu A
IuA
Adamovskaia
V G
VG
Dymokovskaia
S V
SV
D002846:Chromatography, Affinity; D005670:Fusarium; D008970:Molecular Weight; D009181:Mycoses / Q000188:drug therapy*; D020156:Salicylic Acid / Q000627:therapeutic use*; D014908:Triticum / Q000187:drug effects* / Q000201:enzymology; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D014361:Trypsin Inhibitors; D020156:Salicylic Acid
Ingibitory tripsina iz infitsirovannykh i obrabotannykh salitsilovoĭ kislotoĭ prorostkov pshenitsy.