title: Nucleoli, rRNA genes and ITS region in Posidonia oceanica (L.) Delile.
abstract: The maximum number of nucleoli was counted in interphase nuclei of Posidonia oceanica, and a restriction pattern of nuclear rDNA was obtained after digestion with four restriction endonucleases and Southern hybridization. P. oceanica has only one type of ribosomal gene whose size was estimated to be 18.5 kbp long. The nucleotide sequence of the entire ITS region was also determined by direct sequencing of PCR amplified DNA fragments. The sequence of the ITS region was aligned with those of homologous regions of other monocots available in literature, and phylogenetic trees were obtained.
authors.affiliation: Dip. Agrobiologia ed Agrochimica Università La Tuscia, Viterbo, Italia.
authors.forename: G F
authors.initials: GF
authors.lastname: Tucci
authors.forename: R I
authors.initials: RI
authors.lastname: De Dominicis
authors.forename: A G
authors.initials: AG
authors.lastname: Ficca
authors.forename: M
authors.initials: M
authors.lastname: Celi
authors.forename: C
authors.initials: C
authors.lastname: Gregori
mesh_terms: D001483:Base Sequence; D002466:Cell Nucleolus / Q000235:genetics*; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics*; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics*; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010944:Plants / Q000235:genetics*; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D015183:Restriction Mapping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D018749:RNA, Plant; D012335:RNA, Ribosomal; D012340:RNA, Ribosomal, 5.8S; C065039:RNA, ribosomal, 25S