Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris.
The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alphaC, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose.
Hochstrasser
U
U
Department of Botany, University of Basel, Switzerland.
Lüscher
M
M
De Virgilio
C
C
Boller
T
T
Wiemken
A
A
D020121:5' Untranslated Regions; D018076:DNA, Complementary / Q000032:analysis; D005822:Genetic Vectors; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D006602:Hexosyltransferases / Q000235:genetics*; D001467:Hordeum / Q000201:enzymology* / Q000235:genetics; D010843:Pichia / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D014162:Transfection; D014170:Transformation, Genetic; D043324:beta-Fructofuranosidase
D020121:5' Untranslated Regions; D018076:DNA, Complementary; D013395:Sucrose; D006602:Hexosyltransferases; C097181:sucrose - fructan 6-fructosyltransferase; D006026:Glycoside Hydrolases; D043324:beta-Fructofuranosidase