title: A simple procedure for solid-phase synthesis of peptide nucleic acids with N-terminal cysteine.
abstract: Problems were encountered during attempts to prepare N-terminal cysteine-substituted peptide nucleic acids (PNAs) from commercially available, Fmoc-protected monomers. These problems have been surmounted by the use of an S-t-butylmercapto protecting group on the cysteine moiety. The solid-phase syntheses are carried out via a simplified procedure which should be generally useful for manual PNA synthesis.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
authors.forename: T E
authors.initials: TE
authors.lastname: Goodwin
authors.forename: R D
authors.initials: RD
authors.lastname: Holland
authors.forename: J O
authors.initials: JO
authors.lastname: Lay
authors.forename: K D
authors.initials: KD
authors.lastname: Raney
mesh_terms: D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D012116:Resins, Plant; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D012116:Resins, Plant; D003545:Cysteine