PCR primer evaluation for the detection of trypanosome DNA in naturally infected goats.
The buffy coat of 76 roaming goats from the Bansang and Missira regions in Gambia, was examined for the presence of trypanosomes. From these animals, extractions from dry blood samples on filter paper were subjected to PCR using three different primer sets, ORPHON5J, GOL and TVW, specific for Trypanosoma brucei/Trypanosoma evansi, Trypanosoma congolense and Trypanosoma vivax, respectively. PCR results for T. congolense were 100% concordant with buffy coat examination. Besides the three T. vivax buffy coat-positive samples, another 15 yielded positive with the TVW primers. The ORPHON5J primers yielded no positive results. Analyses with the GOL primers of putatively negative samples, yielded aberrant band patterns whose diagnostic significance still remains to be determined.
Pereira de Almeida
P J
PJ
Institute of Tropical Medicine, Antwerp, Belgium. palmeid@itg.be
Ndao
M
M
Goossens
B
B
Osaer
S
S
D000818:Animals; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000097:blood*; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005714:Gambia / Q000453:epidemiology; D015511:Goat Diseases / Q000175:diagnosis* / Q000453:epidemiology; D006041:Goats; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D014345:Trypanosoma / Q000235:genetics* / Q000302:isolation & purification; D014353:Trypanosomiasis, African / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*
D017931:DNA Primers; D016054:DNA, Protozoan