title: Adaptation of the fluorogenic 5'-nuclease chemistry to a PCR-based reverse transcriptase assay.
authors.affiliation: FDA, Bethesda, MD 20892, USA.
authors.forename: T
authors.initials: T
authors.lastname: Maudru
authors.forename: K W
authors.initials: KW
authors.lastname: Peden
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral / Q000032:analysis; D005456:Fluorescent Dyes / Q000737:chemistry*; D015497:HIV-1 / Q000235:genetics / Q000302:isolation & purification*; D007700:Kinetics; D009052:Leukemia Virus, Murine / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D012680:Sensitivity and Specificity; D019914:Taq Polymerase / Q000737:chemistry*
chemical_list: D020121:5' Untranslated Regions; D017931:DNA Primers; D018076:DNA, Complementary; D004279:DNA, Viral; D005456:Fluorescent Dyes; D019914:Taq Polymerase