Promoter trapping identifies real genes in C. elegans.
Promoter trapping involved screening uncharacterized fragments of C. elegans genomic DNA for C. elegans promoter activity. By sequencing the ends of these DNA fragments and locating their genomic origin using the available genome sequence data, promoter trapping has now been shown to identify real promoters of real genes, exactly as anticipated. Developmental expression patterns have thereby been linked to gene sequence, allowing further inferences on gene function to be drawn. Some expression patterns generated by promoter trapping include subcellular details. Localization to the surface of particular cells or even particular aspects of the cell surface was found to be consistent with the genes, now associated with these patterns, encoding membrane-spanning proteins. Data on gene expression patterns are easier to generate and characterize than mutant phenotypes and may provide the best means of interpreting the large quantity of sequence data currently being generated in genome projects.
Hope
I A
IA
School of Biology, The University of Leeds, UK. i.a.hope@leeds.ac.uk
Arnold
J M
JM
McCarroll
D
D
Jun
G
G
Krupa
A P
AP
Herbert
R
R
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D015870:Gene Expression; D017238:Genes, Helminth; D017930:Genes, Reporter; D005821:Genetic Techniques; D008957:Models, Genetic; D010957:Plasmids; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D012984:Software; D001616:beta-Galactosidase
D001616:beta-Galactosidase