title: In situ detection of apoptotic cells by TUNEL in the gill epithelium of the developing brown trout (Salmo trutta).
abstract: Apoptosis is a form of naturally occurring cell death during development and it is characterised by extensive DNA fragmentation. Apoptosis is easily detected in the gill epithelium of brown trout embryos in ultrathin sections (Rojo et al. 1997). Here we provide the first biochemical evidence for apoptosis in the gill epithelium of brown trout embryos, using in situ end-labelling of DNA breaks (Gavrieli et al. 1992). Embryos at d 57 of development as well as those at hatching, were processed to analyse the distribution of apoptotic cells in the gills. The extent of apoptosis revealed by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labelling method technique is considerably greater than apoptosis detected by nuclear morphology. This method revealed that apoptosis was frequent at hatching, although it was also present during embryonic development. The presence and distribution of stained nuclei were different depending on the developmental stage. In embryos of 57 d, apoptotic flattened nuclei were dispersed in the gill epithelium, whereas at hatching, they were mainly grouped in the tips of the filaments and in the gill arches. TUNEL also revealed a distinct pattern of nuclear staining: at hatching, the intense staining covered the entire cell, but in embryos it was restricted to the nucleus. These results show the functional relevance of apoptosis at hatching, when apoptosis seems to be the unique process by which cell numbers in the gill epithelium are adjusted, in order to prepare for the new extrinsic conditions affecting the free-living life of alevins.
authors.affiliation: Department of Anatomy and Comparative Pathological Anatomy, Veterinary Faculty, UCM, Madrid, Spain.
authors.forename: M C
authors.initials: MC
authors.lastname: Rojo
authors.forename: M E
authors.initials: ME
authors.lastname: Gonzalez
mesh_terms: D000818:Animals; D017209:Apoptosis; D004848:Epithelium / Q000196:embryology; D005880:Gills / Q000196:embryology*; D020287:In Situ Nick-End Labeling; D014337:Trout / Q000196:embryology*