title: FAD-mediated enzymatic conversion of NAD+ to NADH: application to chiral synthesis of L-lactate.
abstract: Electroenzymatic reduction of NAD+ to NADH for subsequent use in enzymatic synthesis has been carried out at carbon electrodes bearing lipoamide dehydrogenase (LiDH) immobilized under a Nafion film. The self-mediated electron transfer was made possible by an excess of flavin adenine dinucleotide (FAD) entrapped together with LiDH. Results were compared to those obtained with a similar electrode containing both LiDH and a polymeric form of FAD (pFAD) prepared by anodic polymerization of FAD.
authors.affiliation: Fairleigh Dickinson University, Chemistry Department, Teaneck, NJ 07666, USA.
authors.forename: M D
authors.initials: MD
authors.lastname: Leonida
authors.forename: S B
authors.initials: SB
authors.lastname: Sobolov
authors.forename: A J
authors.initials: AJ
authors.lastname: Fry
mesh_terms: D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D005182:Flavin-Adenine Dinucleotide / Q000737:chemistry*; D019344:Lactic Acid / Q000138:chemical synthesis* / Q000737:chemistry; D009243:NAD / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D019344:Lactic Acid; D008058:Dihydrolipoamide Dehydrogenase