Activation and stabilization of chymotrypsin in microdomains of poly(ethylenimine) derivatives. A model of in vivo environment.
Both activity and stability of alpha-Chymotrypsin (ChT) are substantially enhanced in the microdomains of laurylated or benzylated derivatives of poly(ethylenimine). EPR data revealed that the enhancement in activity of ChT is due to increase in the polarity of the microenvironment of Ser-195 caused by complexation of ChT to the polymer derivatives.
Suh
J
J
Department of Chemistry, Seoul National University, Korea.
Lee
Y
Y
Han
S
S
D001593:Benzyl Compounds; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007850:Lauric Acids; D008823:Micelles; D008956:Models, Chemical; D011094:Polyethyleneimine / Q000737:chemistry*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
D001593:Benzyl Compounds; D007850:Lauric Acids; D008823:Micelles; D014443:Tyrosine; C022787:benzoyltyrosine 4-nitroanilide; D011094:Polyethyleneimine; D002918:Chymotrypsin