Rapid urinary iodide test.
Assessment of iodine deficiency and monitoring of iodine supplementation programs demands rapid, simple and cost-effective methods for the determination of urinary iodide concentrations. We propose a rapid test based on the iodide-catalyzed oxidation of 3,3',5,5'-tetramethylbenzidine by peracetic acid/H2O2 to yield colored products. The color of the chemical reaction is compared with color categories of a pictogram corresponding to three ranges (<10, 10-30, and >>30 microg/100 mL) of iodide concentrations. The test is very easy to perform and does not require any instrumentation or apparatus. Sample preparation is simple and consists in the removal of interfering substances by disposable columns, 65 x 10.5 mm, packed with specifically prepared activated charcoal. For comparison with a reference method for measuring urinary iodide (HPLC), we determined the iodide concentrations of 370 random (untimed) urine samples from consecutive patients by both HPLC and the rapid test. The results obtained by both methods are in close agreement with respect to classification of the samples according to the above three ranges, with a maximum difference of <5% for each range. This rapid test is therefore very well suited to epidemiological surveys of iodine deficiency especially in developing countries.
Rendl
J
J
Clinic of Nuclear Medicine, University of Würzburg, Germany. rendl@nuklearmedizin.uni-wuerzburg.de
Bier
D
D
Groh
T
T
Reiners
C
C
D001560:Benzidines; D002851:Chromatography, High Pressure Liquid; D002863:Chromogenic Compounds; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D006861:Hydrogen Peroxide; D007202:Indicators and Reagents; D007454:Iodides / Q000652:urine*; D007455:Iodine / Q000172:deficiency*; D010084:Oxidation-Reduction; D010463:Peracetic Acid; D013053:Spectrophotometry; D013997:Time Factors
D001560:Benzidines; D002863:Chromogenic Compounds; D007202:Indicators and Reagents; D007454:Iodides; C021758:3,3',5,5'-tetramethylbenzidine; D007455:Iodine; D006861:Hydrogen Peroxide; D010463:Peracetic Acid