Alanyl-PNA homoduplex: A-T pairing with the N7-regioisomer of adenine.
The N7-regioisomer of adeninyl alanine can be used as a building block for the synthesis of alanyl-PNA. By changing the nucleobase connectivity from N9 to N7, pairing with the Hoogsteen side is no longer possible and the order of donor/acceptor positions at the Watson-Crick side is reversed. This influences the pairing selectivity but not the stability of alanyl-PNA self-pairing complexes, as shown by UV and CD spectroscopy.
Diederichsen
U
U
Institut für Organische Chemie und Biochemie, TU München, Garching, Germany.
D000225:Adenine / Q000737:chemistry*; D000409:Alanine / Q000737:chemistry*; D001483:Base Sequence; D002942:Circular Dichroism; D007536:Isomerism; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D020135:Peptide Nucleic Acids; D000225:Adenine; D000409:Alanine