Oleoyl-estrone does not alter hypothalamic neuropeptide Y in Zucker lean and obese rats.
Female Zucker lean and obese rats were treated for 14 days with 3.5 micromol/kg oleoyl-estrone (OE) in liposomes (Merlin-2). After 0, 3, 6, 10, and 14 days of treatment, the rats were killed and hypothalamic nuclei (lateral preoptic, median preoptic, paraventricular, ventromedial and arcuate) were used for neuropeptide Y (NPY) radioimmunoassay. In 14 days, OE decreased food intake by 26% in lean and 38% in obese rats and energy expenditure by 6% in lean and 47% in obese rats; the body weight gap between controls and treated rats becoming -17.8% of initial b.wt. in the lean and -13.6% in the obese rats. Obese rats showed higher NPY levels in all the nuclei than the lean rats. Despite a negative energy balance and decreased food intake, there were practically no changes in NPY with OE treatment. The results indicate that oleoyl-estrone does not act through NPY in its control of either food intake or thermogenesis in lean and genetically obese rats.
Cabot
C
C
Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Spain.
Grasa
M M
MM
Adán
C
C
Pérez-Clausell
J
J
Virgili
J
J
Estruch
J
J
Fernández-Löpez
J A
JA
Remesar
X
X
Alemany
M
M
D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001835:Body Weight / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D004734:Energy Metabolism / Q000187:drug effects; D004970:Estrone / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects*; D009478:Neuropeptide Y / Q000032:analysis*; D009765:Obesity / Q000378:metabolism*; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D011924:Rats, Zucker
D019440:Anti-Obesity Agents; D009478:Neuropeptide Y; D009829:Oleic Acids; D004970:Estrone; C108709:oleoyl-estrone