Enhancement of nasal clearance of nontypeable Haemophilus influenzae by oral immunization with outer membrane proteins.
BALB/c mice were orally immunized with liposomes containing outer membrane proteins (OMPs) isolated from nontypeable Haemophilus influenzae (NTHi) and GM-53 as an adjuvant daily for 14 days. Anti-OMP IgA antibody titers in nasal wash, saliva, and fecal extract were significantly increased after the immunization. Although anti-OMP IgM and IgG antibodies were detected in serum, serum IgA antibodies specific to OMPs were not found. Enzyme-linked immunospot assay showed an increased number of OMP-specific IgA-secreting cells in nasal passages, intestinal lamina propria lymphocytes, and the spleen. Following oral immunization, a suspension of live NTHi was injected into the nose; nasal washes were collected 12 h after the inoculation. The number of NTHi in nasal washes was significantly reduced in mice immunized with liposomes containing OMPs and GM-53 compared to that in mice immunized with liposomes containing GM-53 alone. There was a significant negative correlation between the number of NTHi and anti-OMP IgA antibody titers in nasal washes. These findings suggest that antigen-specific IgA responses in the nose can be induced by oral immunization with OMPs and might be associated with the ability to clear NTHi from the nose.
Suzuki
M
M
Department of Otolaryngology, Oita Medical University, Japan.
Kurono
Y
Y
Kodama
S
S
Shigemi
H
H
Mogi
G
G
D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood / Q000276:immunology; D000921:Antibody-Producing Cells / Q000276:immunology; D000942:Antigens, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage* / Q000276:immunology; D015169:Colony Count, Microbial; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D006193:Haemophilus influenzae / Q000276:immunology* / Q000502:physiology; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood / Q000276:immunology; D007413:Intestinal Mucosa / Q000276:immunology; D008081:Liposomes; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009079:Mucociliary Clearance / Q000276:immunology*; D017817:Nasal Lavage Fluid / Q000276:immunology / Q000382:microbiology; D009297:Nasal Mucosa / Q000276:immunology; D009666:Nose / Q000276:immunology / Q000382:microbiology*; D012463:Saliva / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D008081:Liposomes