T cells mediate treatment of six-day-old cytokine-gene-transfected mouse mammary tumor.
We have previously shown that immunologically different mouse mammary cancer cell lines induce antitumor responses after IL2 or IL4 gene transfection. We now report the ability of cytokine-transfected tumors to induce eradication of established wild-type tumor. Animals with 6-day-old tumor treated with IL2-transfected cells also had significantly smaller tumors 2.8 and 1.7 cm2 (EMT6 and 410.4). Findings were similar for IL4-transfected cells. Tumor infiltrating lymphocytes or cells from draining lymph nodes demonstrated tumor-specific in vitro cytotoxicity. Immunohistochemical studies revealed T cell infiltrates in transfected tumors.
Matory
Y L
YL
Department of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Mass. 02115, USA. YMatory@bics.bwh.harvard.edu
Dorfman
D M
DM
Chen
M
M
Wu
L
L
Spanjaard
R A
RA
Goedegebuure
P S
PS
Eberlein
T J
TJ
D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D005822:Genetic Vectors / Q000008:administration & dosage; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007150:Immunohistochemistry; D007156:Immunologic Memory; D007376:Interleukin-2 / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D015847:Interleukin-4 / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008325:Mammary Neoplasms, Experimental / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014162:Transfection / Q000379:methods*; D014407:Tumor Cells, Cultured
D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D007376:Interleukin-2; D015847:Interleukin-4