Catalase-positive Eikenella corrodens and Eikenella-like isolates of human and canine origin.
Ten catalase-positive isolates and one catalase-negative isolate that had been assigned to Eikenella corrodens were compared to the nomenclatural type strain regarding selected phenotypic and molecular features and chromosomal deoxyribonucleic acid (DNA) relatedness using the spectrophotometric method. Five catalase-positive human isolates were assigned to the genomic species Eikenella corrodens on the basis of high DNA relatedness levels. Three others, among them strain Chen UB 204, exhibited only moderate degrees of DNA relatedness to the type strain and with each other. Two catalase-positive isolates from dogs were closely interrelated, but yielded only low degrees of DNA binding with Eikenella corrodens and the Eikenella-like human isolates. These findings confirm that the human eikenellas comprise more than one genomic species and that the canine strains represent a distinct taxonomic entity. The differentiation of the strains investigated by conventional phenotypic features, hydrolytic enzyme reactions, and cellular carbohydrate patterns was considered.
Kasten
R
R
Institute of Medical Microbiology and Hospital Hygiene, Philipps University Marburg, Germany.
Mutters
R
R
Mannheim
W
W
D000818:Animals; D015373:Bacterial Typing Techniques; D002374:Catalase / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D016970:Eikenella / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D016124:Eikenella corrodens / Q000145:classification* / Q000201:enzymology / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D006801:Humans; D009055:Mouth / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D010641:Phenotype
D004269:DNA, Bacterial; D002374:Catalase