Feline calicivirus capsid protein expression and capsid assembly in cultured feline cells.
The capsid protein of feline calicivirus (FCV) was expressed by using plasmids containing cytomegalovirus, simian virus 40, or T7 promoters. The strongest expression was achieved with the T7 promoter and coinfection with vaccinia virus expressing the T7 RNA polymerase (MVA/T7pol). The FCV precursor capsid protein was processed to the mature-size protein, and these proteins were assembled in to virus-like particles.
Geissler
K
K
Institute for Medical Microbiology, Infectious and Epidemic Diseases, Ludwig Maximilians University Munich, 80539 Munich, Germany.
Schneider
K
K
Fleuchaus
A
A
Parrish
C R
CR
Sutter
G
G
Truyen
U
U
D000818:Animals; D017927:Calicivirus, Feline / Q000502:physiology*; D002213:Capsid / Q000502:physiology*; D002415:Cats; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D051379:Mice; D011498:Protein Precursors / Q000378:metabolism; D014709:Vero Cells; D019065:Virus Assembly
D011498:Protein Precursors