Interaction between sodium ascorbate and dopamine.
The interaction between sodium ascorbate and dopamine was investigated by three different parameters: radical intensity, prooxidant action, and cytotoxicity induction. Sodium ascorbate and dopamine produced the doublet and quartet ESR signals under alkaline conditions (pH 8.0-9.5), respectively. Addition of increasing concentrations of sodium ascorbate completely scavenged the dopamine radical and replaced the latter with its own radical. Similarly, dopamine slightly, but significantly reduced the radical intensity of sodium ascorbate. These two compounds stimulated the methionine oxidation and hydrogen peroxide generation in culture medium, but in combination, their stimulation activities were weakened. Both of these two compounds dose-dependently reduced the viable cell number of human oral squamous carcinoma HSC-4 cells, and their cytotoxic activity was significantly reduced by catalase. When these two compounds were mixed together before adding to HSC-4 cells, both of their cytotoxic activities were diminished. The present study demonstrates the interaction between sodium ascorbate and dopamine, which might modify their biological activities and generation of nerve disorders such as Parkinson's disease.
Sakagami
H
H
Department of Dental Pharmacology, Meikai University School of Dentistry, Sakado, Saitama, Japan. sakagami@dent.meikai.ac.jp
Satoh
K
K
Ida
Y
Y
Hosaka
M
M
Arakawa
H
H
Maeda
M
M
D000596:Amino Acids / Q000032:analysis; D001205:Ascorbic Acid / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D016166:Free Radical Scavengers / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D008715:Methionine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D010084:Oxidation-Reduction; D010300:Parkinson Disease / Q000378:metabolism; D014407:Tumor Cells, Cultured
D000596:Amino Acids; D016166:Free Radical Scavengers; D005609:Free Radicals; D008715:Methionine; D006861:Hydrogen Peroxide; D002374:Catalase; D001205:Ascorbic Acid; D004298:Dopamine; C013111:methionine sulfoxide