title: Seroprevalence and field isolation of bovine immunodeficiency virus.
abstract: A seroprevalence study of bovine lentivirus, known as bovine immunodeficiency virus (BIV), was conducted in 12 different dairy herds in Hokkaido, where some herds were a high prevalence of bovine leukemia virus (BLV) infection. Amongst 611 cattle, 28.6% of cattle were BLV-seropositive, and 11.7% of cattle were seropositive for BIV, while 4.2% of cattle were seropositive for both BIV and BLV. For the isolation of BIV, 19 samples of peripheral blood mononuclear cells (PBMC) and one sample of milk-derived leukocytes were prepared from BIV-seropositive cows. These PBMC and leukocyte preparations were then co-cultivated with cc81 cells, a cat cell line transformed by mouse sarcoma virus. BIV was isolated from 17 PBMC and one milk-derived leukocyte samples. The isolated viruses showed slow replication and syncytia formation. Major core antigen, p26 from these isolates were reacted with anti-BIV (American isolate R-29) serum. In addition, proviral DNA was detected in blood and milk samples by nested polymerase chain reaction and subsequent Southern blot hybridization. Nucleotide sequence analysis of the amplified pol gene products showed its 99.0 to 99.7% homology to that of BIV R-29. These results indicate that the Japanese BIV isolates appear to be antigenically and genetically similar to the American R-29. Since BIV was isolated from milk samples, BIV could possibly be transmitted through milk. This is the first report of BIV isolation in Japan.
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Meas
authors.forename: H
authors.initials: H
authors.lastname: Kabeya
authors.forename: S
authors.initials: S
authors.lastname: Yoshihara
authors.forename: K
authors.initials: K
authors.lastname: Ohashi
authors.forename: S
authors.initials: S
authors.lastname: Matsuki
authors.forename: Y
authors.initials: Y
authors.lastname: Mikami
authors.forename: C
authors.initials: C
authors.lastname: Sugimoto
authors.forename: M
authors.initials: M
authors.lastname: Onuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001483:Base Sequence; D015153:Blotting, Western / Q000662:veterinary; D002415:Cats; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D018920:Coculture Techniques / Q000662:veterinary; D004279:DNA, Viral / Q000737:chemistry; D016583:Enzootic Bovine Leukosis / Q000453:epidemiology; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D016087:Immunodeficiency Virus, Bovine / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology; D005779:Immunodiffusion / Q000662:veterinary; D016180:Lentivirus Infections / Q000453:epidemiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000302:isolation & purification; D007963:Leukocytes, Mononuclear / Q000821:virology; D008892:Milk / Q000821:virology; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000662:veterinary; D009053:Sarcoma Viruses, Murine; D016036:Seroepidemiologic Studies; D019562:Viral Load; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D004279:DNA, Viral