[Morphological observation of combined-culture of tendon cell or fibroblast of rabbit with artificial materials in vitro].
In order to investigate the compatibility and growth between the tendon cell or fibroblast of rabbit and artificial materials, the combined-culture of the two cells with the carbon fiber, terylene and chitin was observed respectively. Results showed as following: in vitro, the compatibility of carbon fiber with these two cells was well, cell-adhesion ability was good as well. Few cells grew on terylene. Chitin inhibited the growth of either cells. No matter the tendon cell or the fibroblast, the amount of cells adhering on the carbon fiber was far more than that on terylene or chitin. When the three materials were interlaced together, the collagen fibers produced by the cells were arranged in direction parallel to the carbon fibers. As the time elapsed, the cells on the carbon fiber distributed evenly and enveloped the material in network-like fashion, this suggested that carbon fiber was a good material for producing living artificial tendon and ligament.
Zhang
Q
Q
Department of Orthopedic Surgery, 1st University Hospital, West China University of Medical Sciences, Chengdu, Sichuan.
Yang
Z
Z
Peng
W
W
D000818:Animals; D002244:Carbon; D000077482:Carbon Fiber; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000166:cytology; D019736:Prostheses and Implants; D011817:Rabbits; D013710:Tendons / Q000166:cytology*
D000077482:Carbon Fiber; D002244:Carbon; D003094:Collagen