Transcription factor binding to the core promoter of the human monoamine oxidase B gene in the cerebral cortex and in blood cells.
Many studies show that monoamine oxidase B in blood cells is a biological marker for personality characteristics such as sensation seeking. The mechanism underlying this association is so far not explored. In the present study we have performed electrophoretic mobility-shift assays to investigate the pattern of protein binding to a 150 bp fragment of the proximal 5'-flanking region of the human monoamine oxidase B gene. We compared the pattern using nuclear extracts from human brain and lymphocytes. Interestingly, a correlation was observed between monoamine oxidase B enzyme activity in blood cells (platelets) and the binding pattern of two uncharacterized transcription factors. These data are well in line with the long-standing notion that interindividual differences in platelet monoamine oxidase may represent differences in expression of the enzyme rather than genotypic variation.
Ekblom
J
J
Department of Neuroscience, Uppsala University, Sweden.
Garpenstrand
H
H
Damberg
M
M
Chen
K
K
Shih
J C
JC
Oreland
L
L
D001792:Blood Platelets / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000201:enzymology*; D005786:Gene Expression Regulation; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*
D016329:Sp1 Transcription Factor; D014157:Transcription Factors; D008995:Monoamine Oxidase