Deconvolution of chemiluminescent emission curve associated with phagocytosis into three logarithmic normal distributions.
The chemiluminescent emission reaction dependence on the activity of phagocytosis is well known. However, this method is not used to diagnostically in clinical assessment because the relationship between phagocytizing activity and chemiluminescent intensity has not been clearly established. Therefore, we attempted to analyze quantitatively the chemiluminescent emission curve by the phagocytosis of leukocytes. Mathematical assessment of the emission curve with respect to time was performed by fitting the curve to several regression models using the unweighed non-linear least squares method. A triple logarithmic normal distribution model provided a reasonable goodness of fit to the measured emission curve. The first component, about 5% of the calculated total counts, was assumed to arise from monocytes activity, the second component, about 20% from eosinocytes activity and the third component, up to 75%, from neutrophils activity. This method seems promising as a means for assaying whole blood without the need for pretreatment and for the providing a valid index that is independent of the technical differences between laboratories.
Okazaki
N
N
Department of Anesthesiology and Reanimatology, Faculty of Medicine, Tottori University, Yonago, Japan. nokzk@grape.med.tottori-u.ac.jp
Hirosawa
J
J
Saito
N
N
Sato
T
T
D003627:Data Interpretation, Statistical; D006531:HEPES; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008163:Luminescent Measurements; D008165:Luminol; D008954:Models, Biological; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010101:Oxygen Consumption; D010587:Phagocytosis / Q000276:immunology*; D015054:Zymosan
D008165:Luminol; D015054:Zymosan; D006531:HEPES