Enzymatic alpha(2-3)sialylation of non-natural type-I (Lewisc) disaccharides with recombinant sialyl-transferase.
Recombinant alpha(2-3)sialyl-transferase from rat liver is used to sialylate a series of type-I (Lewisc) disaccharides on a preparative scale. The enzyme tolerates a broad array of N-acetyl replacements of the N-glucosamine subunit ranging from small and large lipophilic groups to charged and heterocyclic amides.
Baisch
G
G
Novartis Pharma AG, Basle, Switzerland.
Ohrlein
R
R
Streiff
M
M
D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012799:Sialyltransferases / Q000737:chemistry*
D004187:Disaccharides; D011994:Recombinant Proteins; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid