title: Substrate specificity of human prolyl-4-hydroxylase.
abstract: Proline analogs (3-F, 3-Cl, 3-Br, 3,3-cyclopropyl, 3,3-methylene, 3-Me, and 4-Me) were synthesized, incorporated into CbzGlyPheXGlyOEt, and tested as substrate analogues/mechanistic probes for the human prolyl-4-hydroxylase catalyzed hydroxylation reaction. With the exception of the 3-fluoroproline containing peptide, none of these peptides were substrates for the enzyme.
authors.affiliation: Department of Chemistry, Cornell University, Ithaca, New York 14853, USA.
authors.forename: M
authors.initials: M
authors.lastname: Tandon
authors.forename: M
authors.initials: M
authors.lastname: Wu
authors.forename: T P
authors.initials: TP
authors.lastname: Begley
authors.forename: J
authors.initials: J
authors.lastname: Myllyharju
authors.forename: A
authors.initials: A
authors.lastname: Pirskanen
authors.forename: K
authors.initials: K
authors.lastname: Kivirikko
mesh_terms: D006801:Humans; D006900:Hydroxylation; D007202:Indicators and Reagents; D015394:Molecular Structure; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D011392:Proline / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D007202:Indicators and Reagents; D011392:Proline; D011393:Procollagen-Proline Dioxygenase