title: Delta opiate receptors account for the castration-induced unmasking of gonadotropin-releasing hormone binding sites in the rat pituitary.
abstract: Under control incubation conditions, gonadotropin-releasing hormone (GnRH) binds only a fraction of its receptors in rat-cultivated pituitary cells. Unmasking of the remaining receptors, which have been termed 'cryptic', requires drug- or peptide-induced protein kinase activation. Spontaneous masking however is not observed on pituitary cells sampled from castrated male rats, suggesting the presence of an intrinsic unmasking factor. Many endogenous factors could theoretically account for the effect. Here we attempted to identify the factor involved by taking advantage of their differential dependency upon second messengers and transduction cascades. Spontaneous unmasking of GnRH binding was found reversed by pertussis toxin (PTX), an inhibitor of alphai and alphao subunits of heterotrimeric G proteins, and by U73122, a phospholipase C (PLC) inhibitor. In contrast, desensitization of protein kinase C (PKC) or inhibition of tyrosine kinase by herbimycin were ineffective. Among endogenous pituitary factors able to unmask GnRH receptors in pituitary cells from normal male rats, as EGF, NPY or opiate peptides, only the latter were found to correspond to this transduction profile. In an attempt to characterize the pharmacology of opiate effects, naloxone (10 microM), a poorly selective opiate antagonist, restored masking of GnRH binding in cells from castrates. Only the delta antagonist naltrindole (1 microM) was able to mimick the action of naloxone. Conversely, when tested on cells from intact animals, morphine (10 microM), as well as dslet (1 microM) and met-ENK (10 nM), preferential delta agonists, but not dago and beta-endorphin or U50488 H and dynorphin, respectively micro and kappa agonists, were able to suppress masking. Among opioid peptides endogenous to the pituitary, only met-ENK was able to unmask cryptic receptors, an effect antagonized by naltrindole. We conclude that an opiate delta receptor subtype is endogenously activated in the pituitary of castrated male rats to prevent masking of GnRH binding.
authors.affiliation: Unité de recherche sur la Dynamique des Systèmes Neuroendocriniens, INSERM U159, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Leblanc
authors.forename: A
authors.initials: A
authors.lastname: L'Héritier
authors.forename: C
authors.initials: C
authors.lastname: Kordon
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D002478:Cells, Cultured; D017077:Culture Media, Conditioned; D008024:Ligands; D008297:Male; D009478:Neuropeptide Y / Q000494:pharmacology; D018847:Opioid Peptides / Q000494:pharmacology; D009919:Orchiectomy; D010902:Pituitary Gland / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D011966:Receptors, LHRH / Q000378:metabolism*; D017465:Receptors, Opioid, delta / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000963:Antimetabolites; D017077:Culture Media, Conditioned; D008024:Ligands; D009478:Neuropeptide Y; D018847:Opioid Peptides; D011966:Receptors, LHRH; D017465:Receptors, Opioid, delta