Influenza virus hemagglutinin stimulates the protein kinase C activity of human polymorphonuclear leucocytes.
Human polymorphonuclear leukocytes (PMNL) incubated with influenza virus, A/USSR/90/77 (H1N1) or its hemagglutinin produced a significant increase in their PKC activity when compared with untreated PMNL. The activated kinase translocated from cytosol to cellular membrane. The calcium-dependent enzyme activity was inhibited by a specific inhibitor suggesting that alpha and/or beta isoforms of PKC were involved.
Arora
D J
DJ
Virology Research Center, Institut Armand-Frappier, Laval, Québec, Canada.
Gasse
N
N
D000818:Animals; D002118:Calcium / Q000502:physiology; D002462:Cell Membrane / Q000201:enzymology; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000494:pharmacology*; D006801:Humans; D009504:Neutrophils / Q000201:enzymology*; D011493:Protein Kinase C / Q000187:drug effects* / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011493:Protein Kinase C; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium