Partial purification and characterization of a novel murine factor that augments the expression of class I MHC antigens on tumor cells.
A soluble factor which augments the expression of major histocompatibility complex class I (MHC I) antigens on a number of murine tumor cell lines, has been isolated from the culture supernatants of mixed lymphocyte reaction of spleen cells derived from C57B1/6, Balb/c and Swiss mice. The factor, termed MHC-augmenting factor (MHC-AF) has been partially purified by Sephadex G-100 column chromatography and reverse phase HPLC. MHC-AF activity is associated with an 18 kDa molecule. MHC-AF activity was resistant to pH 2.0 treatment and partially purified MHC-AF preparations did not have any activity in L929 cell/vesicular stomatitis virus (VSV) interferon bioassay system. Antibodies to IFN-gamma did not block the activity of MHC-AF. These results indicate that a MHC-AF distinct from IFN-gamma, is produced by mouse spleen cells undergoing a mixed lymphocyte reaction.
Puri
N
N
School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Saxena
R K
RK
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D002918:Chymotrypsin / Q000737:chemistry / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D015395:Histocompatibility Antigens Class I / Q000187:drug effects / Q000378:metabolism*; D006358:Hot Temperature; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D014357:Trypsin / Q000737:chemistry / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000276:immunology
D000906:Antibodies; D015395:Histocompatibility Antigens Class I; D011506:Proteins; D003208:Concanavalin A; D007371:Interferon-gamma; D002918:Chymotrypsin; D014357:Trypsin