title: Inhibitory innervation of the guinea-pig urethra; roles of CO, NO and VIP.
abstract: The inhibitory innervation of guinea-pig urethral smooth muscle was investigated histochemically and functionally. The distribution of immunoreactivities to haem oxygenases (HO), neuronal NO synthase (nNOS), and vasoactive intestinal polypeptide (VIP) was studied, and the functional effects of the corresponding putative transmitters, CO, NO, and VIP, were assessed. HO-2 immunoreactivity was found in all nerve cell bodies of intramural ganglia, localized between smooth muscle bundles in the detrusor, bladder base and proximal urethra. About 70% of the ganglionic cell bodies were also NOS-immunoreactive (IR), whereas a minor part was VIP-IR. Some ganglion cells exhibiting tyrosine hydroxylase (TH) activity were demonstrated. Rich numbers of NOS-IR varicose nerve terminals could be found innervating the smooth muscle of the urethra, whereas VIP-IR terminals were less numerous. A rich number of TH-IR terminals were observed. The bladder showed a similar distribution of nerves, although only a few number of TH-IR nerves could be found. In bladder preparations exposed to sodium nitroprusside, cGMP-IR cells could be seen, forming an interconnecting network with long spindle-shaped processes. The cGMP-IR cells were especially abundant in the outer smooth muscle layers of the bladder, but less numerous in the urethra. In urethral strip preparations, electrical field stimulation evoked long-lasting frequency-dependent relaxations. The relaxations were not inhibited by the NO-synthesis inhibitor, L-NOARG, or enhanced by the NO-precursor, L-arginine. The haem precursor, 5-aminolevulinic acid (5-ALA), or the inhibitor of guanylate cyclase, ODQ, did not affect the urethral relaxations. Exogenously applied NO, SIN-1, and VIP relaxed the preparations by approximately 50%, whereas the relaxation evoked by exogenous CO was minor. These results suggest that CO probably is not involved in non-adrenergic, non-cholinergic inhibitory control of the guinea-pig urethra, where a non-NO/cGMP mediated relaxation seems to be predominant.
authors.affiliation: Department of Clinical Pharmacology, Lund University Hospital, Sweden.
authors.forename: V
authors.initials: V
authors.lastname: Werkström
authors.forename: P
authors.initials: P
authors.lastname: Alm
authors.forename: K
authors.initials: K
authors.lastname: Persson
authors.forename: K E
authors.initials: KE
authors.lastname: Andersson
mesh_terms: D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology / Q000502:physiology*; D006152:Cyclic GMP / Q000378:metabolism; D004558:Electric Stimulation; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D005725:Ganglia, Autonomic / Q000166:cytology / Q000502:physiology; D006168:Guinea Pigs; D006419:Heme Oxygenase (Decyclizing) / Q000378:metabolism; D007150:Immunohistochemistry; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009569:Nitric Oxide / Q000494:pharmacology / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052248:Nitric Oxide Synthase Type I; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors; D014521:Urethra / Q000187:drug effects / Q000294:innervation* / Q000502:physiology; D001743:Urinary Bladder / Q000294:innervation / Q000502:physiology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology / Q000502:physiology*; D014966:Xanthenes
chemical_list: D004791:Enzyme Inhibitors; D009419:Nerve Tissue Proteins; D014966:Xanthenes; D009569:Nitric Oxide; D014660:Vasoactive Intestinal Peptide; C002385:linsidomine; D002248:Carbon Monoxide; C034657:Texas red; D008981:Molsidomine; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; D006419:Heme Oxygenase (Decyclizing); D000254:Sodium-Potassium-Exchanging ATPase; D006152:Cyclic GMP