Susceptibility of erythrocyte lipids to oxidation and erythrocyte antioxidant status in myocardial infarction.
OBJECTIVE
We evaluated the erythrocyte lipid susceptibility to oxidation and erythrocyte antioxidant status in patients with myocardial infarction (MI).


METHODS
Patients with MI were divided into two group according to the severity of the disease as severe (n = 30) or milder (n = 25). Malondialdehyde as a marker of lipid peroxidation was measured to show the lipid susceptibility to oxidation. Erythrocytes were stressed in vitro by hydrogen peroxide acting as the oxidative agents for 120 min. After designated time, erythrocyte MDA production was significantly higher in patients with severe MI than in controls and in patients with milder MI (p < 0.001, p < 0.001, respectively). Antioxidant status was determined by measuring the reduced glutathione levels and glutathione peroxidase activity of erythrocyte.


RESULTS
In patients with MI, antioxidant status was significantly lower than in controls, and there was no significant difference between the patient groups.


CONCLUSIONS
Determination of erythrocyte lipid susceptibility to oxidation may be a useful in vitro test to evaluate the degree of oxidative stress in myocardial infarction.
Konukoğlu
D
D
Department of Biochemistry Cerrahpaşa Medical Faculty, Istanbul, Turkey.
Akçay
T
T
Erdem
T
T
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000097:blood; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009203:Myocardial Infarction / Q000378:metabolism*; D018384:Oxidative Stress
D000975:Antioxidants; D008054:Lipid Peroxides; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D005978:Glutathione