[Establishment of mutant strain from human lung cancer cell (A549) with characteristics of resistance to 8-AG and HGPRT deficiency].
A human lung cancer cell line (A549) mutant with hypoxanthine guanine phosphoribosyltransferase (HGPRT) deficiency and resistance to 8-AG was established by treatment of the A549 cells with 3 micrograms.ml-1 N-methyl-N'-nitro-nitrosoguanidine (MNNG), and prescreened in 0.25% agarose DMEM semisolid medium containing 20 micrograms.ml-1 8-AG. The mutant cells are resistant to cytotoxic effect of 8-AG and sensitive to hypoxanthine-aminopterin thymidine (HAT) selective medium. The mutant cells can be used as a candidate parental cells to fuse with the somatic cells.
Xiao
Y
Y
Department of Microbiology, Hunan Medical University, Changsha.
Hu
C
C
Shi
K
K
Luo
M
M
D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D001375:Azaguanine / Q000494:pharmacology*; D002459:Cell Fusion; D002461:Cell Line, Transformed; D019008:Drug Resistance, Neoplasm; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000172:deficiency*; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D008769:Methylnitronitrosoguanidine; D009154:Mutation; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D008769:Methylnitronitrosoguanidine; D007041:Hypoxanthine Phosphoribosyltransferase; D001375:Azaguanine