title: Cell yield and superoxide dismutase activity of the marine yeast Debaryomyces hansenii under different culture conditions.
abstract: The effect of aeration, pH, stirring rate, and temperature on the biomass production and superoxide dismutase (SOD) activity of the marine yeast Debaryomyces hansenii strain C-11 was determined. The cell biomass yield was approximately 50% in a seawater-formulated medium using glucose as the carbon source. The SOD activity increased by application of a pulse of oxygen or 0.8 mM sulfate copper into the chemical reactor. The SOD enzyme had an activity of 400 units/mg of protein in a crude extract produced under such conditions, the best activity ever reported for this enzyme in a crude preparation.
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, División de Biologia Experimental, Unidad de Patologia Marina, A.P. 128, La Paz 23000, Baja California Sur, México
authors.lastname: Ramírez Orozco M
authors.initials: NY
authors.lastname: Hernández-Saavedra
authors.lastname: Ascencio Valle F
authors.lastname: Acosta González B
authors.initials: JL
authors.lastname: Ochoa