title: Globin chain synthesis analysis by high performance liquid chromatography in the screening of thalassemia syndromes.
abstract: We applied reversed phase high performance liquid chromatography for globin chain synthesis analysis in screening for beta-thalassemia. The alpha/non-alpha-globin chain synthesis ratios have been determined in alpha-, beta-, and deltabeta-thalassemia carriers using the classical carboxymethyl cellulose chromatography as the reference method. Reversed phase high performance liquid chromatography is fast, accurate, and reproducible, and may be a suitable alternative for the traditional carboxymethyl cellulose chromatography.
authors.affiliation: Istituto di Clinica e Biologia dell'Età Evolutiva, Università degli Studi di Cagliari Ospedale Regionale Microcitemie, Italia.
authors.forename: R
authors.initials: R
authors.lastname: Galanello
authors.forename: S
authors.initials: S
authors.lastname: Satta
authors.forename: M G
authors.initials: MG
authors.lastname: Pirroni
authors.forename: M
authors.initials: M
authors.lastname: Travi
authors.forename: L
authors.initials: L
authors.lastname: Maccioni
mesh_terms: D002266:Carboxymethylcellulose Sodium; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes; D005069:Evaluation Studies as Topic; D005914:Globins / Q000096:biosynthesis* / Q000737:chemistry; D006801:Humans; D017086:beta-Thalassemia / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D005914:Globins; D002266:Carboxymethylcellulose Sodium