Mutagenicity of microcystin-LR in human RSa cells.
Microcystin-LR (MCLR) is a potent cyclic heptapeptidic hepatotoxin produced by bloom-forming cyanobacteria. The mutagenicity induced by this compound in cultured human RSa cells was found by determination of ouabain-resistant (OuaR) mutation, with the highest frequency at the concentration of 15 microgram/ml. Moreover, base substitution mutations at K-ras codon 12 in genomic DNA, assessed by polymerase chain reaction (PCR) and differential dot-blot hybridization using digoxigenin-labeled probes, were detected in RSa cells 6 days after exposure to MCLR (7.5-15 microgram/ml).
Suzuki
H
H
Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Shinjuku-ku, Tokyo 169-0073, Japan.
Watanabe
M F
MF
Wu
Y
Y
Sugita
T
T
Kita
K
K
Sato
T
T
Wang
X
X
Tanzawa
H
H
Sekiya
S
S
Suzuki
N
N
D002478:Cells, Cultured; D004351:Drug Resistance / Q000235:genetics; D011905:Genes, ras / Q000187:drug effects; D006801:Humans; D008387:Marine Toxins; D052998:Microcystins; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D010042:Ouabain / Q000494:pharmacology; D010456:Peptides, Cyclic / Q000633:toxicity*
D008387:Marine Toxins; D052998:Microcystins; D009153:Mutagens; D010456:Peptides, Cyclic; D010042:Ouabain; C057862:cyanoginosin LR