Plasma copolymer surfaces of acrylic acid/1,7 octadiene: surface characterisation and the attachment of ROS 17/2.8 osteoblast-like cells.
The purpose of this study was: (a) to examine the effect of plasma-gas composition on plasma polymer oxygen/carbon (O/C) ratio, functional group composition and stability in water, and then (b) to examine cell attachment to surfaces containing different concentrations of O/C and functional groups. Oxygen-functionalised surfaces were deposited by means of the plasma copolymerisation of acrylic acid/1,7-octadiene. The use of a diluent hydrocarbon allowed the deposition of surfaces with a range of O/C concentrations. Plasma copolymer surfaces were characterised by X-ray photoelectron spectroscopy (XPS). Changes in functional group composition with % acrylic acid monomer and the non-dispersive and dispersive parts of the surface energy of these plasma copolymers were measured. The solubility of the plasma copolymers was assessed by means of XPS. The degree of attachment of ROS 17/2.8 osteoblast-like cells to plasma copolymer surfaces deemed to be 'stable' in aqueous medium was measured. Tissue culture polystyrene (TCPS) was included as a control. Attachment was found to be greatest to the plasma copolymer surface with an O/C of 0.11. This surface had a carboxylic acid concentration of ca. 3%. Attachment did not correlate with increased surface wettability (i.e. the non-dispersive component of the surface energy).
Daw
R
R
Centre for Biomaterials and Tissue Engineering, Department of Engineering Materials, University of Sheffield, UK.
Candan
S
S
Beck
A J
AJ
Devlin
A J
AJ
Brook
I M
IM
MacNeil
S
S
Dawson
R A
RA
Short
R D
RD
D000179:Acrylates / Q000737:chemistry*; D000466:Alkadienes / Q000737:chemistry*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D005740:Gases; D010006:Osteoblasts / Q000166:cytology*; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties; D014014:Tissue Adhesives / Q000737:chemistry*; D014407:Tumor Cells, Cultured
D000179:Acrylates; D000466:Alkadienes; D001672:Biocompatible Materials; D005740:Gases; D011108:Polymers; D014014:Tissue Adhesives; C036658:acrylic acid