Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation.
BACKGROUND
Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.


METHODS
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.


RESULTS
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.


CONCLUSIONS
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.
Yamagami
S
S
Department of Ophthalmology, Jichi Medical School, Tochigi, Japan. yamagami@vision.eri.harvard.edu
Kawashima
H
H
Endo
H
H
Tsuru
T
T
Shibui
H
H
Kagawa
Y
Y
Hori
J
J
Yamagami
H
H
Isobe
M
M
D000818:Animals; D001082:Aqueous Humor / Q000737:chemistry* / Q000166:cytology; D003315:Cornea / Q000737:chemistry / Q000378:metabolism; D016039:Corneal Transplantation / Q000276:immunology / Q000473:pathology; D016207:Cytokines / Q000235:genetics*; D015870:Gene Expression; D006084:Graft Rejection / Q000235:genetics; D007150:Immunohistochemistry; D007371:Interferon-gamma / Q000235:genetics; D016753:Interleukin-10 / Q000235:genetics; D007376:Interleukin-2 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D016207:Cytokines; D007376:Interleukin-2; D012333:RNA, Messenger; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma