The presenilin 2 loop domain interacts with the mu-calpain C-terminal region.
Presenilin 2 (PS2) is a gene responsible for the early-onset familial Alzheimer's disease (AD). PS2 mutations are considered to be closely related to the pathogenesis of AD. We screened for proteins that interact with PS2 to understand its pathological and physiological functions. Using the PS2 loop domain as the bait, the yeast two-hybrid system was used for screening, and mu-calpain was identified as a PS2 binding protein. In COS-1 cells, the interaction of PS2 with mu-calpain was confirmed by immunoprecipitation. These results suggested that PS2 and mu-calpain interact with each other, and might regulate each other's functions.
Shinozaki
K
K
Laboratory of Neurochemistry, National Institute for Physiological Sciences, Myodaiji, Okazaki, Aichi 444, Japan.
Maruyama
K
K
Kume
H
H
Tomita
T
T
Saido
T C
TC
Iwatsubo
T
T
Obata
K
K
D000818:Animals; D019556:COS Cells; D002154:Calpain / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011233:Precipitin Tests; D053766:Presenilin-2; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000032:analysis / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000235:genetics
D004792:Enzyme Precursors; D008565:Membrane Proteins; C508861:PSEN2 protein, human; D053766:Presenilin-2; D011993:Recombinant Fusion Proteins; D002154:Calpain; C098484:mu-calpain proenzyme