Effect induced by interleukin-1 on the behaviour of B16F10 melanoma cells.
We have previously demonstrated that in vitro treatment with interleukins (IL-2 and IL-6) enhances metastasis in B16 murine melanoma. IL-1 has been reported to be involved in the metastatic activity of human melanoma; so in the present study we have investigated if IL-1 could also modulate the behaviour of this experimental tumor. Data showed that low doses of IL-1 induced cell proliferation and several modifications in the expression of MHC antigens, fibronectin and laminin, however these changes did not lead to a higher metastatic efficiency. In addition it was shown that B16F10 cells express IL-1 mRNA. It can be concluded that IL-1 may be involved in the biology of B16F10 cells although its role in metastasis needs further investigation.
De Galdeano
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AG
Department of Cell Biology and Morphological Sciences, School of Medicine and Dentistry, University of the Basque Country, Leioa 48940, Vizcaya, Spain.
Boyano
D
D
Cañavate
L
L
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D005353:Fibronectins / Q000096:biosynthesis / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology* / Q000502:physiology; D007797:Laminin / Q000096:biosynthesis / Q000235:genetics; D008114:Liver Neoplasms, Experimental / Q000556:secondary; D008175:Lung Neoplasms / Q000556:secondary; D008297:Male; D008546:Melanoma, Experimental / Q000378:metabolism / Q000473:pathology* / Q000556:secondary; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D009368:Neoplasm Transplantation; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured / Q000187:drug effects
D005353:Fibronectins; D006183:H-2 Antigens; D007375:Interleukin-1; D007797:Laminin; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm