Identification of drug metabolites in biological matrices by intelligent automated liquid chromatography/tandem mass spectrometry.
A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography-tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates intelligent realtime mass spectrometry with HPLC detection and a predictive strategy for detecting metabolites arising from common biotransformations, to rapidly elucidate structures of drug metabolites. Structures of metabolites generated from in vitro incubation mixtures of Indinavir were characterized from a single chromatographic analysis using the automated LC/MS/MS methodology, thus reducing data acquisition time and improving efficiency.
Lopez
L L
LL
ThermoQuest Finnigan, San Jose, CA 95134, USA.
Yu
X
X
Cui
D
D
Davis
M R
MR
D000818:Animals; D019380:Anti-HIV Agents / Q000032:analysis* / Q000493:pharmacokinetics; D001322:Autoanalysis; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D017320:HIV Protease Inhibitors / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D066298:In Vitro Techniques; D019469:Indinavir / Q000032:analysis* / Q000493:pharmacokinetics; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D013058:Mass Spectrometry; D051381:Rats; D013347:Subcellular Fractions / Q000737:chemistry / Q000378:metabolism
D019380:Anti-HIV Agents; D017320:HIV Protease Inhibitors; D019469:Indinavir