title: Molecular interactions between Vestigial and Scalloped promote wing formation in Drosophila.
abstract: Scalloped (Sd) and Vestigial (Vg) are each needed for Drosophila wing development. We show that Sd is required for Vg function and that altering their relative cellular levels inhibits wing formation. In vitro, Vg binds directly to both Sd and its human homolog, Transcription Enhancer Factor-1. The interaction domains map to a small region of Vg that is essential for Vg-mediated gene activation and to the carboxy-terminal half of Sd. Our observations indicate that Vg and Sd function coordinately to control the expression of genes required for wing development, which implies that Vg is a tissue-specific transcriptional intermediary factor of Sd.
authors.affiliation: Banting and Best Department of Medical Research, Charles H. Best Institute, University of Toronto, Ontario M5G 1L6, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Simmonds
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: K H
authors.initials: KH
authors.lastname: Soanes
authors.forename: H M
authors.initials: HM
authors.lastname: Krause
authors.forename: K D
authors.initials: KD
authors.lastname: Irvine
authors.forename: J B
authors.initials: JB
authors.lastname: Bell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019521:Body Patterning; D017124:Conserved Sequence; D004268:DNA-Binding Proteins / Q000378:metabolism; D019281:Dimerization; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D017930:Genes, Reporter / Q000235:genetics; D018398:Homeodomain Proteins / Q000378:metabolism; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D017384:Sequence Deletion; D000091262:TEA Domain Transcription Factors; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation; D014921:Wings, Animal / Q000196:embryology*; D015003:Yeasts
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D019476:Insect Proteins; D009687:Nuclear Proteins; D000091262:TEA Domain Transcription Factors; C057182:TEAD1 protein, human; D014157:Transcription Factors; C087984:sd protein, Drosophila; C095064:vg protein, Drosophila