alpha-Mannosidase activity from antibody raised against a glucal antigen.
Sixty cell lines of monoclonal antibody were raised against a glucal hapten 1. Among them, Ab 405.4 showed alpha-mannosidase activity as kcat = 0.19/day (kcat/kuncat = 110,000). The chemical modification study and pH profile study of this antibody indicated that carboxyl group(s) in the antigen binding site involved in catalytic mechanism.
Yu
J
J
Division of Applied Science, Korea Institute of Science & Technology, Seoul, Korea.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002125:Calcium Gluconate / Q000737:chemistry / Q000276:immunology*; D002384:Catalysis; D002460:Cell Line; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008361:Mannosidases / Q000378:metabolism*; D015394:Molecular Structure; D043323:alpha-Mannosidase
D000911:Antibodies, Monoclonal; D006241:Haptens; D008361:Mannosidases; D043323:alpha-Mannosidase; D002125:Calcium Gluconate