Patterns of the immunohistochemical expression of melanoma-associated antigens and density of CD45R0+ activated T lymphocytes and L1-protein positive macrophages in primary cutaneous melanomas.
There is ample evidence for spontaneous antimelanoma immune reactivity mediated by melanocyte-differentiation-antigens (MDAs). Our aim was to determine whether MDA immunoreactivity is associated with increased tumour-infiltrating lymphocytes (TIL) and macrophages (TIM). A retrospective study was conducted in 30 medium and high grade primary cutaneous melanomas (PCM) as identified by CART-analysis. All of the cases had developed clinical evidence for metastasis within 3 years following surgical excision of the PCM. We used immunohistochemistry and computerized image analysis to quantify MDAs positive cells (Melan A/MART-1, gp100/Pmel 17/HMB45, tyrosinase), CD45R0-positive TIL and LI-protein-positive TIM. A stochastic relationship was present between the MDA immuno-reactivities and the densities in TIL and TIM. An inverse relationship was yielded between TIL and TIM. No specific pattern of PCM immunoreactivity for MDAs, TIL and TIM was found to predict metastases.
Piérard-Franchimont
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Department of Dermatopathology, University of Liège, Belgium.
Letawe
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Nikkels
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Piérard
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D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D002454:Cell Differentiation; D006801:Humans; D007150:Immunohistochemistry; D017493:Leukocyte Common Antigens / Q000378:metabolism; D039841:Leukocyte L1 Antigen Complex; D058965:MART-1 Antigen; D008264:Macrophages / Q000276:immunology*; D008544:Melanocytes / Q000276:immunology; D008545:Melanoma / Q000276:immunology* / Q000473:pathology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D014442:Monophenol Monooxygenase / Q000276:immunology / Q000378:metabolism; D009363:Neoplasm Proteins / Q000276:immunology / Q000378:metabolism; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D011506:Proteins / Q000276:immunology / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*; D058951:gp100 Melanoma Antigen
D000951:Antigens, Neoplasm; D015295:Antigens, Tumor-Associated, Carbohydrate; D039841:Leukocyte L1 Antigen Complex; D058965:MART-1 Antigen; C548667:MLANA protein, human; D008562:Membrane Glycoproteins; D009363:Neoplasm Proteins; D019006:Neural Cell Adhesion Molecules; C548637:PMEL protein, human; D011506:Proteins; D058951:gp100 Melanoma Antigen; D014442:Monophenol Monooxygenase; D017493:Leukocyte Common Antigens