Coat protein-mediated resistance to pea enation mosaic virus in transgenic Pisum sativum L.
Pea (Pisum sativum L.) plants were transformed in planta by injection/electroporation of axillary meristems with a chimeric pea enation mosaic virus (PEMV) coat protein gene contruct. R1 progenies of these plants were shown to harbor the transgene by polymerase chain reaction (PCR) and genomic Southern analysis, while transgene expression was demonstrated by western blot analysis. Transgenic R2, R3 and R4 plants displayed delayed or transient PEMV multiplication and attenuated symptoms as compared to control inoculated individuals.
Chowrira
G M
GM
Department of Genetics and Cell Biology, Washington State University, Pullman 99164-4234, USA.
Cavileer
T D
TD
Gupta
S K
SK
Lurquin
P F
PF
Berger
P H
PH
D001483:Base Sequence; D002213:Capsid / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D005814:Genes, Viral; D009029:Mosaic Viruses / Q000235:genetics* / Q000472:pathogenicity*; D018532:Pisum sativum / Q000235:genetics* / Q000821:virology*; D010641:Phenotype; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic; D014774:Virulence / Q000235:genetics
D017931:DNA Primers