Hydrolase-catalyzed beta-mannosylations.
Transmannosylations catalysed by beta-mannosidase from snail viscera or beta-galactosidase from Aspergillus oryzae were accomplished with 4-nitrophenyl beta-D-mannopyranoside as donor substrate. With suitable hydrophobic acceptor molecules preferentially beta1-4-linked disaccharides were obtained. The activities of both glycosidases in buffer cosolvent mixtures were determined, and conditions for their immobilization were elaborated and optimized. A model of the enzymic transfer mechanism is suggested.
Taubken
N
N
Institut für Organische Chemie, Universität Hamburg, Germany.
Thiem
J
J
D000818:Animals; D001236:Aspergillus oryzae / Q000201:enzymology; D004187:Disaccharides / Q000096:biosynthesis* / Q000737:chemistry; D004800:Enzymes, Immobilized; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006027:Glycosides / Q000737:chemistry; D006031:Glycosylation; D006863:Hydrogen-Ion Concentration; D008361:Mannosidases / Q000378:metabolism; D008362:Mannosides / Q000096:biosynthesis* / Q000737:chemistry; D008956:Models, Chemical; D019906:Nuclear Magnetic Resonance, Biomolecular; D012908:Snails / Q000201:enzymology; D014781:Viscera / Q000201:enzymology; D001616:beta-Galactosidase / Q000378:metabolism; D044902:beta-Mannosidase
D004187:Disaccharides; D004800:Enzymes, Immobilized; D006027:Glycosides; D008362:Mannosides; D006026:Glycoside Hydrolases; D008361:Mannosidases; D001616:beta-Galactosidase; D044902:beta-Mannosidase