Photomechanical transcutaneous delivery of macromolecules.
Transcutaneous drug delivery has been the subject of intensive research. In certain situations, rapid transcutaneous delivery is very desirable. A mechanical (stress) pulse generated by a single laser pulse was shown to transiently increase the permeability of the stratum corneum in vivo. The barrier function of the stratum corneum recovers within minutes. The increased permeability during these few minutes allows macromolecules to diffuse through the stratum corneum into the viable epidermis and dermis. Macromolecules (40 kDa dextran and 20 nm latex particles) were deposited into the skin using a photomechanical pulse generated by a single 23 ns laser pulse. This treatment can potentially be utilized in therapies that currently require occlusive dressings for hours or day(s).
Lee
S
S
Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston 02114, USA.
McAuliffe
D J
DJ
Flotte
T J
TJ
Kollias
N
N
Doukas
A G
AG
D000818:Animals; D003911:Dextrans / Q000008:administration & dosage; D016503:Drug Delivery Systems / Q000379:methods*; D004817:Epidermis / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010784:Photomicrography; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D003911:Dextrans; D046911:Macromolecular Substances