Nonrestricted differential intoxication of cells by pertussis toxin.
After uptake and retrograde transport pertussis toxin acts by ADP-ribosylating alpha-Gi proteins. We show that uptake via many different receptor proteins followed by retrograde transport and intoxication is not restricted to a particular cell type. The efficiency of cellular intoxication, however, was found to be cell type dependent.
el Bayâ
A
A
Institut für Infektiologie - Zentrum für Molekularbiologie der Entzündung, Westfälische Wilhelms-Universität Münster, Germany.
Brückener
K
K
Schmidt
M A
MA
D000246:Adenosine Diphosphate Ribose / Q000378:metabolism; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D016466:CHO Cells; D018938:Caco-2 Cells; D002352:Carrier Proteins / Q000378:metabolism; D002477:Cells / Q000187:drug effects* / Q000378:metabolism; D006224:Cricetinae; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects; D006367:HeLa Cells; D006801:Humans; D009439:Neuraminidase / Q000378:metabolism; D037342:Pertussis Toxin; D011956:Receptors, Cell Surface / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D013997:Time Factors; D010566:Virulence Factors, Bordetella / Q000378:metabolism / Q000633:toxicity*
D002352:Carrier Proteins; D006023:Glycoproteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D010566:Virulence Factors, Bordetella; D000246:Adenosine Diphosphate Ribose; D037342:Pertussis Toxin; D009439:Neuraminidase; D019206:GTP-Binding Protein alpha Subunits, Gi-Go