[Changes of NMDA receptor gene expression in infection brain injury induced by Bordetella pertussis in rats].
N-methyl-D-aspartate receptors (NMDAR) in cerebral cortexes of rat brain injury induced by Bordetella pertussis (Bp) were measured by methods of Northern blot analysis and [3H] MK-801 radioligand-receptor binding to study changes and effects of NMDAR1 of gene expression in the infection brain injury. The affinity (Kd) of NMDAR in Bp group was significantly decreased [30.5 +/- 3.0 nmol.L-1 (Bp), 43.1 +/- 4.2 nmol.L-1 (NS), P < 0.05], but expression of NMDAR1 mRNA and binding site density (Bmax) of NMDAR observed no significant differences between the experimental and the control group (P > 0.05), respectively. The results suggest that NMDAR1 was activated with high affinity functionally, but no changes in binding site density and transcriptional expression of NMDAR1 occured in the infection brain injury.
Chen
X
X
Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha.
Yang
Y
Y
Du
J
J
Tao
Y
Y
Cheng
G
G
D000818:Animals; D001665:Binding Sites; D001885:Bordetella Infections / Q000378:metabolism*; D001886:Bordetella pertussis; D002540:Cerebral Cortex / Q000378:metabolism*; D004660:Encephalitis / Q000378:metabolism* / Q000382:microbiology; D015870:Gene Expression; D008297:Male; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000096:biosynthesis* / Q000235:genetics
D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate