Role of alpha-dystroglycan as a Schwann cell receptor for Mycobacterium leprae.
alpha-Dystroglycan (alpha-DG) is a component of the dystroglycan complex, which is involved in early development and morphogenesis and in the pathogenesis of muscular dystrophies. Here, alpha-DG was shown to serve as a Schwann cell receptor for Mycobacterium leprae, the causative organism of leprosy. Mycobacterium leprae specifically bound to alpha-DG only in the presence of the G domain of the alpha2 chain of laminin-2. Native alpha-DG competitively inhibited the laminin-2-mediated M. leprae binding to primary Schwann cells. Thus, M. leprae may use linkage between the extracellular matrix and cytoskeleton through laminin-2 and alpha-DG for its interaction with Schwann cells.
Rambukkana
A
A
Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY 10021, USA. rambuka@rockvax.rockefeller.edu
Yamada
H
H
Zanazzi
G
G
Mathus
T
T
Salzer
J L
JL
Yurchenco
P D
PD
Campbell
K P
KP
Fischetti
V A
VA
D000818:Animals; D001422:Bacterial Adhesion; D001665:Binding Sites; D002118:Calcium / Q000502:physiology; D002461:Cell Line, Transformed; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism* / Q000494:pharmacology; D049030:Dystroglycans; D004492:Edetic Acid / Q000494:pharmacology; D006031:Glycosylation; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism* / Q000494:pharmacology; D009166:Mycobacterium leprae / Q000378:metabolism*; D010525:Peripheral Nerves / Q000737:chemistry; D051381:Rats; D017474:Receptors, Laminin / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism / Q000382:microbiology*
D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D007797:Laminin; D008562:Membrane Glycoproteins; D017474:Receptors, Laminin; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D049030:Dystroglycans; D004492:Edetic Acid; D002118:Calcium