Characterization of oligosaccharides from an antigenic mannan of Saccharomyces cerevisiae.
Mannans of the yeast Saccharomyces cerevisiae have been implicated as containing the allergens to which bakers and brewers are sensitive and also the antigen recognized by patients with Crohn's disease. A fraction of S. cerevisiae mannan, Sc500, having high affinity for antibodies in Crohn's patients has been characterized by NMR spectroscopy followed by fragmentation using alkaline elimination, partial acid hydrolysis and acetolysis. The released oligosaccharides were separated by gel filtration on a Biogel P4 column and analyzed by fluorescence labeling, HPLC and methylation analysis. The relationship between structure and antigen activity was measured by competitive ELISA. The antigenic activity of the original high molecular weight mannan could be ascribed to terminal Manalpha1-->3Manalpha1-->2 sequences which are rarely found in human glycoproteins but were over-represented in Sc500 compared to other yeast mannans.
Young
M
M
Department of Biochemistry and Molecular Biology, University College London, UK.
Davies
M J
MJ
Bailey
D
D
Gradwell
M J
MJ
Smestad-Paulsen
B
B
Wold
J K
JK
Barnes
R M
RM
Hounsell
E F
EF
D000946:Antigens, Fungal / Q000737:chemistry*; D002240:Carbohydrate Sequence; D003424:Crohn Disease / Q000276:immunology; D006801:Humans; D006868:Hydrolysis; D008351:Mannans / Q000737:chemistry*; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D009844:Oligosaccharides / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000276:immunology
D000946:Antigens, Fungal; D008351:Mannans; D009844:Oligosaccharides