Determination of 6s-trans conformation of retinal chromophore in sensory rhodopsin I and phoborhodopsin.
8,16-Ethanoretinal 3 was synthesized from 2-methylcyclohexanone. From the binding experiments of 3 and 8,18-ethanoretinal 2 with apoproteins of sensory rhodopsin I and phoborhodopsin, it was found that retinal was incorporated as 6s-trans conformation in the both proteins.
Wada
A
A
Kobe Pharmaceutical University, Japan.
Akai
A
A
Goshima
T
T
Takahashi
T
T
Ito
M
M
D019843:Archaeal Proteins; D001436:Bacteriorhodopsins / Q000737:chemistry* / Q000378:metabolism; D002338:Carotenoids; D006217:Halobacterium / Q000378:metabolism; D025602:Halorhodopsins; D012172:Retinaldehyde / Q000737:chemistry*; D025603:Sensory Rhodopsins; D013237:Stereoisomerism
D019843:Archaeal Proteins; D025602:Halorhodopsins; C061497:SRI protein, Halobacterium; D025603:Sensory Rhodopsins; C061508:sensory rhodopsin II protein, archaeal; D002338:Carotenoids; D001436:Bacteriorhodopsins; D012172:Retinaldehyde