[Identification and expression of ribulose 1,5-bisphosphate carboxylase/oxygenase gene from Thiobacillus versutus].
The chromosomal DNA of Thiobacillus versutus was hybridized with various heterologous ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) gene as probes. Only Rhodobacter sphaeroides form I RubisCO gene showed homology with T. versutus. The RubisCO gene fragment of T. versutus was isolated using the RubisCO gene of R. sphaeroides as a probe. And the RubisCO gene of T. versutus can express in E. coli cell.
Liu
Z
Z
State Key Laboratory of Microbial Technology, Shandong University, Jinan.
Yan
W
W
D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics; D012273:Ribulose-Bisphosphate Carboxylase / Q000096:biosynthesis* / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid; D013855:Thiobacillus / Q000145:classification / Q000201:enzymology / Q000235:genetics*
D004269:DNA, Bacterial; D012273:Ribulose-Bisphosphate Carboxylase