Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate.
A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs = 1. 83) on 5 microns octadecylsilan column using 35% acetonitrile in 0.05 M ammonium acetate buffer (pH = 6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N = 3).
Kim
K H
KH
College of Pharmacy, Kangwon National University, Chunchon, Korea.
Kim
T K
TK
D000085:Acetates; D000318:Adrenergic beta-Agonists / Q000652:urine*; D000420:Albuterol / Q000652:urine*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D017879:Isothiocyanates / Q000737:chemistry*; D013696:Temperature
D000085:Acetates; D000318:Adrenergic beta-Agonists; D002021:Buffers; D007202:Indicators and Reagents; D017879:Isothiocyanates; C041943:2,3,4,6-tetra-O-acetylglucopyranosylisothiocyanate; D000420:Albuterol; C018824:ammonium acetate