CRP.cAMP-dependent transcription activation of the Escherichia coli pts Po promoter by the heat shock RNA polymerase (Esigma32) in vitro.
The P0 promoter of the Escherichia coli pts operon that is activated during growth in the presence of glucose contains a recognition sequence for the RNA polymerase holoenzyme containing sigma 70 (Esigma70) which is the RNA polymerase holoenzyme responsible for the transcription of heat shock genes, and a putative recognition element for Esigma32, the Esigma32 was found to be able to transcribe from the pts P0 promoter with in vitro transcription assays using purified RNA polymerase holoenzymes. Esigma32 and Esigma70 used the same transcriptional start site and both RNA polymerases were activated in the presence of a cAMP receptor protein (CRP) and cAMP complex (CRP.cAMP). These results suggest the possibility that the contact between CRP.cAMP and the alpha subunit of the RNA polymerase can be made regardless of the kind of sigma subunit that is associated with the core RNA polymerase.
Ryu
S
S
Department of Microbiology, College of Medicine, Chungbuk National University, Chongju, Korea. sryu@med.chungbuk.ac.kr
D001483:Base Sequence; D000242:Cyclic AMP / Q000378:metabolism; D002373:Cyclic AMP Receptor Protein / Q000378:metabolism*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D014158:Transcription, Genetic; D015533:Transcriptional Activation
D002373:Cyclic AMP Receptor Protein; D004269:DNA, Bacterial; D006360:Heat-Shock Proteins; D012808:Sigma Factor; D014157:Transcription Factors; C069774:heat-shock sigma factor 32; D000242:Cyclic AMP; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases