Antisense inhibition of expression of cysteine proteinases affects Entamoeba histolytica-induced formation of liver abscess in hamsters.
Trophozoites of virulent Entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (CP5) have only 10% of the CP activity but retain their cytopathic activity on mammalian monolayers. In the present study we found that the transfected trophozoites with low levels of CP activity were incapable of inducing the formation of liver lesions in hamsters.
Ankri
S
S
Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
Stolarsky
T
T
Bracha
R
R
Padilla-Vaca
F
F
Mirelman
D
D
D000818:Animals; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000096:biosynthesis / Q000235:genetics*; D015853:Cysteine Proteinase Inhibitors / Q000235:genetics* / Q000502:physiology; D004748:Entamoeba histolytica / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D004749:Entamoebiasis / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D005786:Gene Expression Regulation; D008101:Liver Abscess, Amebic / Q000201:enzymology / Q000469:parasitology* / Q000473:pathology; D008647:Mesocricetus; D016372:RNA, Antisense / Q000502:physiology*; D014162:Transfection
D015853:Cysteine Proteinase Inhibitors; D016372:RNA, Antisense; D003546:Cysteine Endopeptidases