SLE autoantibodies binding to native calf thymus DNA brominated in high salt.
Native calf thymus DNA was brominated in high salt to achieve B-->Z conformational transition. Ultraviolet and circular dichroism spectroscopic studies point towards the conformational modification of the native DNA. Specific binding of the monoclonal anti-Z-DNA antibody (Z-22) to the DNA brominated in high salt further confirmed the B-->Z conformational isomerization of native DNA. The role of Z-DNA in the etiopathogenesis of systemic lupus erythematosus has been investigated in the light of the binding of naturally occurring human anti-DNA autoantibodies to the induced Z-DNA.
Moinuddin
Department of Biochemistry, Faculty of Medicine, AMU, Aligarh, India.
Arjumand
S
S
Ali
A
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000378:metabolism; D001323:Autoantibodies / Q000737:chemistry* / Q000378:metabolism*; D001666:Binding Sites, Antibody; D001966:Bromine; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013950:Thymus Gland
D000911:Antibodies, Monoclonal; D001323:Autoantibodies; D004247:DNA; D001966:Bromine