Evidence for an heterogeneous glycosylation of the Clostridium tyrobutyricum ATCC 25755 flagellin.
Glycosylation analysis of the flagellin from the Gram-positive species Clostridium tyrobutyricum has been supplemented. Amino acid analysis of the glycopeptides obtained after pronase digestion of flagellin indicated that O-glycosylation which was previously demonstrated after nonreductive beta-elimination, probably occurred via the hydroxyl group of serine. Otherwise, beta-elimination partly deglycosylated flagellin. After this treatment carbohydrates were still linked to protein as shown by a digoxigenin-hydrazide labelling. Therefore, in addition to linkages via serine, alkaline resistant linkages exist on the flagellin and some glycans may be linked to the protein core via the amide nitrogen of asparagine or via the hydroxyl group of tyrosine. Furthermore, according to an immunological analysis, glycans attached to flagellin via alkaline sensitive linkages may be different from those attached via alkaline resistant linkages.
Bédouet
L
L
Université de Bretagne Occidentale, Institut Universitaire de Technologie de Quimper, Laboratoire Universitaire de Microbiologie Appliquée de Quimper, France.
Arnold
F
F
Robreau
G
G
Batina
P
P
Talbot
F
F
Binet
A
A
D000596:Amino Acids / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000276:immunology; D002236:Carbohydrate Conformation; D002851:Chromatography, High Pressure Liquid; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005408:Flagellin / Q000737:chemistry / Q000378:metabolism*; D006020:Glycopeptides / Q000737:chemistry / Q000276:immunology; D006031:Glycosylation; D011402:Pronase / Q000378:metabolism
D000596:Amino Acids; D000911:Antibodies, Monoclonal; D006020:Glycopeptides; D005408:Flagellin; D011402:Pronase