Recognition of 2'-deoxyisoguanosine triphosphate by HIV-1 reverse transcriptase and mammalian cellular DNA polymerases.
HIV-1 reverse transcriptase (RT) incorporates 2'-deoxyisoguanosine triphosphate (d-isoGTP) opposite thymidine (T) in a DNA template and opposite uracil (U) in an RNA template about 10 times more efficiently than the eukaryotic DNA polymerase alpha, both in the absence and presence of dATP.
Lutz
M J
MJ
Department of Chemistry, ETH Zürich, Universitätstr, Switzerland. michael.lutz@pharma.novartis.com
Horlacher
J
J
Benner
S A
SA
D000241:Adenosine; D001483:Base Sequence; D004247:DNA; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006151:Guanosine / Q000737:chemistry / Q000378:metabolism*; D054303:HIV Reverse Transcriptase / Q000378:metabolism*; D013379:Substrate Specificity; D013698:Templates, Genetic
D006151:Guanosine; C008184:isoguanosine; D004247:DNA; D054303:HIV Reverse Transcriptase; D004259:DNA-Directed DNA Polymerase; D000241:Adenosine