Expression and subcellular distribution of rel/NF kappa B transcription factors in the preimplantation mouse embryo: novel kappa B binding activities in the blastocyst stage embryo.
We describe the expression patterns and subcellular localisation of murine rel/NF kappa B transcription factors and their inhibitors during preimplantation development in the mouse. We find that the known rel and I kappa B proteins are ubiquitously expressed during early murine embryogenesis. The differential persistence of individual rel and I kappa B transcripts in the first cleavage stage embryo and analysis of the 3'UTR suggests that rel and I kappa B protein expression may be regulated at the level of mRNA stability and translation. At the stages of early mouse embryogenesis examined, there was no evidence for strong induction of rel dimer translocation into the nucleus. However, novel kappa B-binding proteins are found in the mouse blastocyst and may provide an alternative mechanism for regulation of gene expression via the kappa B motif in the early mouse embryo.
Parrott
J N
JN
Department of Biochemistry, University of Cambridge, UK.
Gay
N J
NJ
D020413:3' Untranslated Regions / Q000235:genetics; D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics; D005314:Embryonic and Fetal Development; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D020672:I-kappa B Proteins; D007399:Interphase / Q000235:genetics; D051379:Mice; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000235:genetics*; D009687:Nuclear Proteins / Q000032:analysis; D009865:Oocytes / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic / Q000235:genetics; D015053:Zygote / Q000378:metabolism
D020413:3' Untranslated Regions; D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D016328:NF-kappa B; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors