title: Functional heterodimeric amino acid transporters lacking cysteine residues involved in disulfide bond.
abstract: The protein mediating system L amino acid transport, AmAT-L, is a disulfide-linked heterodimer of a permease-related light chain (AmAT-L-lc) and the type II glycoprotein 4F2hc/ CD98. The Schistosoma mansoni protein SPRM1 also heterodimerizes with h4F2hc, inducing amino acid transport with different specificity. In this study, we show that the disulfide bond is formed by heavy chain C109 with a Cys residue located in the second putative extracellular loop of the multi-transmembrane domain light chain (C164 and C137 for XAmAT-L-lc and SPRM1, respectively). The non-covalent interaction of Cys-mutant subunits is not sufficient to allow coimmunoprecipitation, but cell surface expression of the light chains is maintained to a large extent. The non-covalently linked transporters display the same transport characteristics as disulfide bound heterodimers, but the maximal transport rates are reduced by 30-80%.
authors.affiliation: Institute of Physiology, University of Zürich, Switzerland.
authors.forename: R
authors.initials: R
authors.lastname: Pfeiffer
authors.forename: B
authors.initials: B
authors.lastname: Spindler
authors.forename: J
authors.initials: J
authors.lastname: Loffing
authors.forename: P J
authors.initials: PJ
authors.lastname: Skelly
authors.forename: C B
authors.initials: CB
authors.lastname: Shoemaker
authors.forename: F
authors.initials: F
authors.lastname: Verrey
mesh_terms: D026905:Amino Acid Transport Systems; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003545:Cysteine / Q000378:metabolism*; D019281:Dimerization; D004220:Disulfides / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D009865:Oocytes / Q000378:metabolism; D011233:Precipitin Tests; D011487:Protein Conformation; D013329:Structure-Activity Relationship; D014982:Xenopus laevis
chemical_list: D026905:Amino Acid Transport Systems; D002352:Carrier Proteins; D004220:Disulfides; D003545:Cysteine