Chemo-enzymatic synthesis of calcitonin derivatives containing N-linked oligosaccharides.
Eel calcitonin derivatives containing various N-linked oligosaccharides were chemo-enzymatically synthesized by the transglycosylation reaction of Mucor hiemalis endo-beta-N-acetylglucosaminidase (Endo-M) to a glycosylated calcitonin derivative [Asn(GlcNac)3]-CT in which N-acetyl-D-glycosamine (GlcNAc) is attached to the L-asparagine (Asn) residue of the peptide.
Haneda
K
K
Noguchi Institute, Tokyo, Japan.
Inazu
T
T
Mizuno
M
M
Iguchi
R
R
Yamamoto
K
K
Kumagai
H
H
Aimoto
S
S
Suzuki
H
H
Noda
T
T
D000117:Acetylglucosamine / Q000737:chemistry; D000595:Amino Acid Sequence; D001216:Asparagine / Q000737:chemistry; D002116:Calcitonin / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D002851:Chromatography, High Pressure Liquid; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism; D008969:Molecular Sequence Data; D009089:Mucor / Q000201:enzymology; D009844:Oligosaccharides / Q000138:chemical synthesis*
D009844:Oligosaccharides; D001216:Asparagine; D002116:Calcitonin; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D000117:Acetylglucosamine