A vector based on the SV40 origin of replication and chromosomal S/MARs replicates episomally in CHO cells.
We have developed an episomal replicating expression vector in which the SV40 gene coding for the large T-antigen was replaced by chromosomal scaffold/matrix attached regions. Southern analysis as well as vector rescue experiments in CHO cells and in Escherichia coli demonstrate that the vector replicates episomally in CHO cells. It occurs in a very low copy number in the cells and is stably maintained over more than 100 generations without selection pressure.
Piechaczek
C
C
Institut für Zellbiologie, Universität Witten/Herdecke, Stockumer Strasse 10, D-58448 Witten, Germany.
Fetzer
C
C
Baiker
A
A
Bode
J
J
Lipps
H J
HJ
D000818:Animals; D000957:Antigens, Viral, Tumor / Q000235:genetics; D016466:CHO Cells; D006224:Cricetinae; D004261:DNA Replication; D005822:Genetic Vectors / Q000096:biosynthesis*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics; D010957:Plasmids / Q000096:biosynthesis*; D018741:Replication Origin; D013539:Simian virus 40 / Q000235:genetics
D000957:Antigens, Viral, Tumor; D009687:Nuclear Proteins; D016899:Interferon-beta