Protective effects of green tea on hepatotoxicity, oxidative DNA damage and cell proliferation in the rat liver induced by repeated oral administration of 2-nitropropane.
To evaluate the benefit of green tea in mitigating hazards caused by repeated exposure of 2-nitropropane (2NP), we examined the effects of the tea on toxic indices, oxidative DNA damage and cell proliferation in the liver of 2NP-treated rats. Male Fischer 344 rats were administered, by gastric intubation, a total of six doses of 60 mg/kg 2NP(L), or alternatively two doses of 90 mg/kg and then four doses of 120 mg/kg 2NP(H) during 2 weeks. Green tea infusion was given to the rats as drinking water 1 week before the 2NP treatments and throughout the experiment. Significant elevation of hepatotoxic indices was evident in the 2NP(H)-treated group, such as an increase of serum glutamic-oxaloacetic transaminase (GOT) activity and of hepatic lipid peroxidation, together with a decrease in hepatic glycogen and serum triglyceride, and degenerative changes in the hepatocytes. A dose-related increase was observed in oxidative DNA damage and cell proliferation in the liver. Green tea effectively inhibited all of above changes induced by 2NP treatment, suggesting that tea intake may be effective for preventing the hepatic injuries after chronic exposure to 2NP.
Sai
K
K
Division of Cellular and Molecular Toxicology, National Institute of Health Sciences, Tokyo, Japan.
Kai
S
S
Umemura
T
T
Tanimura
A
A
Hasegawa
R
R
Inoue
T
T
Kurokawa
Y
Y
D000818:Animals; D001219:Aspartate Aminotransferases / Q000096:biosynthesis; D002273:Carcinogens / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D004249:DNA Damage / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000517:prevention & control*; D008297:Male; D009595:Nitroparaffins / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D008517:Phytotherapy; D011407:Propane / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D013662:Tea / Q000627:therapeutic use*
D002273:Carcinogens; D009595:Nitroparaffins; D013662:Tea; D001219:Aspartate Aminotransferases; C016617:2-nitropropane; D011407:Propane