title: Characterization of a subunit structure and stability of the recombinant porin from Neisseria gonorrhoeae.
abstract: An outer membrane PIA protein from Neisseria gonorrhoeae strain FA19 was expressed in Escherichia coli and refolded in vitro in the presence of zwitterionic detergent. Its proper folding and subunit organization was confirmed by comparison with the native counterpart. The unfolding of PIA has been investigated using fluorescence spectroscopy and analytical size-exclusion chromatography methods. Analysis of the denaturation pathway of the PIA revealed that it forms an unusually labile quaternary structure. In the presence of 1 M guanidinium chloride (GdmCl) or upon heating up to 50 degrees C, dissociation of the PIA oligomer was observed resulting in the formation of folded monomeric intermediates. Unfolding of monomers occurs at 80 degrees C or in the presence of 4.3 M GdmCl, indicating high intrinsic stability toward both GdmCl and elevated temperatures. Both oligomeric and monomeric forms of PIA exhibited affinity to the hydrophobic probe 1-anilinonaphthalene-8-sulfonic acid (ANS) and bind with Kd=80 and 130 microM, respectively. Denaturation of the PIA completely abolished affinity to ANS, suggesting that hydrophobicity is a property of the folded state of the porin.
authors.affiliation: Department of Protein and Analytical Chemistry, Wyeth-Lederle Vaccines and Pediatrics, West Henrietta, New York 14586, USA. Yury-Matsuka@internetmail.pr.cyanamid.com
authors.forename: Y V
authors.initials: YV
authors.lastname: Matsuka
authors.forename: D A
authors.initials: DA
authors.lastname: Dilts
authors.forename: S
authors.initials: S
authors.lastname: Hoiseth
authors.forename: R
authors.initials: R
authors.lastname: Arumugham
mesh_terms: D002850:Chromatography, Gel; D009344:Neisseria gonorrhoeae; D018272:Porins / Q000737:chemistry* / Q000235:genetics; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D011994:Recombinant Proteins / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D018272:Porins; D011994:Recombinant Proteins; C057699:porin protein, Neisseria