Localization of nitric oxide-synthesizing neurons sending projections to the dorsal raphe nucleus of the rat.
The origin of the nerve fibers immunoreactive for neuronal nitric oxide synthase (nNOS) in the rat dorsal raphe nucleus (DRN) was determined by combining the use of cholera toxin subunit b (CTb) as a retrograde tracer and nNOS immunohistochemistry with a monoclonal anti-nNOS antibody. Double labeled CTb-nNOS cell bodies were distributed from the rostral diencephalon to the caudal medulla oblongata, in about 20 areas of the brain. Several of the areas displaying double labeled cells are known for their involvement in the control of the sleep-wake cycle and/or transmission of nociception.
Léger
L
L
Département de Médecine Expérimentale, INSERM U480, Université Claude Bernard, Lyon, France. leger@sommeil.univ-lyon1.fr
Gay
N
N
Burlet
S
S
Charnay
Y
Y
Cespuglio
R
R
D000204:Activity Cycles / Q000502:physiology; D000818:Animals; D000911:Antibodies, Monoclonal; D002772:Cholera Toxin / Q000032:analysis; D007106:Immune Sera; D007150:Immunohistochemistry; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000378:metabolism* / Q000502:physiology; D009569:Nitric Oxide / Q000096:biosynthesis*; D019001:Nitric Oxide Synthase / Q000032:analysis; D052248:Nitric Oxide Synthase Type I; D011903:Raphe Nuclei / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D051381:Rats
D000911:Antibodies, Monoclonal; D007106:Immune Sera; D009419:Nerve Tissue Proteins; D009569:Nitric Oxide; D002772:Cholera Toxin; D019001:Nitric Oxide Synthase; D052248:Nitric Oxide Synthase Type I; C496322:Nos1 protein, rat