Brazilin inhibits activities of protein kinase C and insulin receptor serine kinase in rat liver.
Hypoglycemic action of brazilin was found to be based on the improvement of peripheral glucose utility, and this action might be correlated with the insulin action pathway. In the present study we investigated the effect of brazilin on the insulin receptor autophosphorylation, protein kinase C (PKC), protein phosphatase and insulin receptor serine kinase in order to confirm whether the hypoglycemic mechanism is concerned with insulin action pathway. Brazilin was found to inhibit PKC and insulin receptor serine kinase, which are involved in the regulation of insulin signal pathway. But any significant effect was not shown on insulin receptor tyrosine kinase activity, autophosphorylation and phosphatase activity. These findings suggest that brazilin might enhance insulin receptor function by decreasing serine phosphorylation, which might mediate hypoglycemic effect of brazilin.
Kim
S G
SG
College of Pharmacy, Seoul National University, Korea.
Kim
Y M
YM
Khil
L Y
LY
Jeon
S D
SD
So
D S
DS
Moon
C H
CH
Moon
C K
CK
D000818:Animals; D001578:Benzopyrans / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D019606:Phosphoamino Acids / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011972:Receptor, Insulin
D001578:Benzopyrans; D004791:Enzyme Inhibitors; D007328:Insulin; D019606:Phosphoamino Acids; D011972:Receptor, Insulin; D017346:Protein Serine-Threonine Kinases; C081827:insulin receptor serine kinase; D011493:Protein Kinase C; D010744:Phosphoric Monoester Hydrolases; C044362:brazilin