The first analogues of LNA (locked nucleic acids): phosphorothioate-LNA and 2'-thio-LNA.
LNA (Locked Nucleic Acids, 1, X = O, Y = O) is a novel oligonucleotide analogue capable of recognizing complementary DNA and RNA with unprecedented thermal affinities. Synthesis of the first chemically modified LNA analogues is reported. A 9-mer phosphorothioate-LNA containing three LNA thymine monomers (1, X = O, Y = S, Base = thymin-1-yl) and 9-mer LNAs containing one, three or five 2'-thio-LNA monomers (1, X = S, Y = O, Base = uracil-1-yl) were able to recognize both complementary DNA and RNA with thermal affinities comparable to those of parent LNA.
Kumar
R
R
Department of Chemistry, University of Copenhagen, Denmark.
Singh
S K
SK
Koshkin
A A
AA
Rajwanshi
V K
VK
Meldgaard
M
M
Wengel
J
J
D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry; D007202:Indicators and Reagents; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D018075:RNA, Complementary / Q000737:chemistry; D013329:Structure-Activity Relationship; D013816:Thermodynamics; D013873:Thionucleotides
D018076:DNA, Complementary; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D018075:RNA, Complementary; D013873:Thionucleotides