Promotion of trophoblast stem cell proliferation by FGF4.
The trophoblast cell lineage is essential for the survival of the mammalian embryo in utero. This lineage is specified before implantation into the uterus and is restricted to form the fetal portion of the placenta. A culture of mouse blastocysts or early postimplantation trophoblasts in the presence of fibroblast growth factor 4 (FGF4) permitted the isolation of permanent trophoblast stem cell lines. These cell lines differentiated to other trophoblast subtypes in vitro in the absence of FGF4 and exclusively contributed to the trophoblast lineage in vivo in chimeras.
Tanaka
S
S
Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
Kunath
T
T
Hadjantonakis
A K
AK
Nagy
A
A
Rossant
J
J
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D019070:Cell Lineage; D002678:Chimera; D017077:Culture Media, Conditioned; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D051519:Fibroblast Growth Factor 4; D005346:Fibroblast Growth Factors / Q000494:pharmacology* / Q000502:physiology; D005347:Fibroblasts / Q000166:cytology; D018507:Gene Expression Regulation, Developmental; D005819:Genetic Markers; D007621:Karyotyping; D008297:Male; D051379:Mice; D008954:Models, Biological; D011518:Proto-Oncogene Proteins / Q000494:pharmacology* / Q000502:physiology; D015398:Signal Transduction; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology* / Q000378:metabolism
D017077:Culture Media, Conditioned; C496446:Fgf4 protein, mouse; D051519:Fibroblast Growth Factor 4; D005819:Genetic Markers; D011518:Proto-Oncogene Proteins; D005346:Fibroblast Growth Factors