title: Validated high-performance liquid chromatographic assay for the determination of promazine in human plasma. Application to pharmacokinetic studies.
abstract: A high-performance liquid chromatographic method for the determination of promazine in human plasma is described. The assay involves a single-step liquid-liquid extraction using pentane-2-propanol (98:2, v/v). The analyte of interest and the internal standard chlorpromazine were separated on a Spherisorb CN column using a mobile phase of acetonitrile-50 mM ammonium acetate (9:1, v/v). Electrochemical detection was achieved using an applied potential of +750 mV. The assay was validated according to international requirements prior to application to a pharmacokinetic study and was found to be specific, accurate and precise with a linear range of 0.25-25 ng ml(-1).
authors.affiliation: Zentrallaboratorium Deutscher Apotheker, Eschborn, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Larsimont
authors.forename: J
authors.initials: J
authors.lastname: Meins
authors.forename: H
authors.initials: H
authors.lastname: Fieger-Büschges
authors.forename: H
authors.initials: H
authors.lastname: Blume
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood* / Q000493:pharmacokinetics; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D008297:Male; D011395:Promazine / Q000097:blood* / Q000493:pharmacokinetics; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D014150:Antipsychotic Agents; D011395:Promazine