A synthetic acceptor substrate for Trypanosoma brucei UDP-Gal: GPI anchor side-chain alpha-galactosyltransferases.
A synthetic analogue of a trisaccharide fragment of the Trypanosoma brucei Variant Surface Glycoprotein (VSG) GlycosylPhosphatidyInositiol (GPI) anchor, Gal-alpha-1,3(Man-alpha-1,6)-Man-alpha-O-octyl (1), serves as a substrate for two T. brucei alpha-galactosyltransferases. The principle tetrasaccharide product derived from (1) contains a Gal-alpha-1,2-Gal linkage.
Brown
J R
JR
School of Chemistry, University of St Andrews, UK.
Smith
T K
TK
Ferguson
M A
MA
Field
R A
RA
D000818:Animals; D001711:Biotransformation; D002240:Carbohydrate Sequence; D005700:Galactosyltransferases / Q000378:metabolism*; D017261:Glycosylphosphatidylinositols / Q000378:metabolism*; D008969:Molecular Sequence Data; D013379:Substrate Specificity; D014346:Trypanosoma brucei brucei / Q000201:enzymology*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism*; D014643:Variant Surface Glycoproteins, Trypanosoma / Q000378:metabolism*
D017261:Glycosylphosphatidylinositols; D014643:Variant Surface Glycoproteins, Trypanosoma; D014531:Uridine Diphosphate Galactose; D005700:Galactosyltransferases