title: EAT-4, a homolog of a mammalian sodium-dependent inorganic phosphate cotransporter, is necessary for glutamatergic neurotransmission in caenorhabditis elegans.
abstract: The Caenorhabditis elegans gene eat-4 affects multiple glutamatergic neurotransmission pathways. We find that eat-4 encodes a protein similar in sequence to a mammalian brain-specific sodium-dependent inorganic phosphate cotransporter I (BNPI). Like BNPI in the rat CNS, eat-4 is expressed predominantly in a specific subset of neurons, including several proposed to be glutamatergic. Loss-of-function mutations in eat-4 cause defective glutamatergic chemical transmission but appear to have little effect on other functions of neurons. Our data suggest that phosphate ions imported into glutamatergic neurons through transporters such as EAT-4 and BNPI are required specifically for glutamatergic neurotransmission.
authors.affiliation: Department of Molecular Biology and Oncology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9148, USA.
authors.forename: R Y
authors.initials: RY
authors.lastname: Lee
authors.forename: E R
authors.initials: ER
authors.lastname: Sawin
authors.forename: M
authors.initials: M
authors.lastname: Chalfie
authors.forename: H R
authors.initials: HR
authors.lastname: Horvitz
authors.forename: L
authors.initials: L
authors.lastname: Avery
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism*; D003001:Cloning, Molecular; D005247:Feeding Behavior / Q000502:physiology; D017238:Genes, Helminth; D005815:Genetic Code; D018698:Glutamic Acid / Q000378:metabolism*; D008465:Mechanoreceptors / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D051381:Rats; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D008565:Membrane Proteins; D050608:Sodium-Phosphate Cotransporter Proteins; D027981:Symporters; D018698:Glutamic Acid