Expression of somatostatin messenger RNA and receptor in cultured brain tumor cells.
In order to understand the role of somatostatin in tumor cell growth, the expression of somatostatin in 7 cultured brain tumor cells was investigated. Radioimmunoassay demonstrated the production of somatostatin into the culture supernatant of 6 tumor cells. The expression of somatostatin mRNA and receptor was investigated by reverse transcription polymerase chain reaction (RT-PCR) and a binding assay with 125I-somatostatin-14, respectively. Only two cell lines, GBS-1 and U87MG, expressed somatostatin mRNA, whereas somatostatin receptor was expressed in all cultured cells. These results suggest that somatostatin is an important factor for regulating cell growth.
Hirota
N
N
Department of Neurosurgery, Showa University School of Medicine, Tokyo, Japan. nobuo@mbn.bb.or.jp
Matsumoto
K
K
Iida
M
M
Sakagami
H
H
Takeda
M
M
D001932:Brain Neoplasms / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism; D006801:Humans; D008527:Medulloblastoma / Q000378:metabolism; D008577:Meningeal Neoplasms / Q000378:metabolism; D008579:Meningioma / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D017481:Receptors, Somatostatin / Q000378:metabolism*; D013004:Somatostatin / Q000235:genetics / Q000378:metabolism*; D013724:Teratoma / Q000378:metabolism; D014407:Tumor Cells, Cultured / Q000378:metabolism
D012333:RNA, Messenger; D017481:Receptors, Somatostatin; D013004:Somatostatin