Establishment of a new human megakaryoblastic cell line, CMY, with chromosome 17p abnormalities.
A new megakaryoblastic cell line CMY was established from a Down's syndrome patient suffering from acute megakaryoblastic leukemia. The karyotypes of CMY showed deletion of chromosome 17 or the translocation of 17p, whereas the blasts of the patient did not reveal these abnormalities of chromosome 17 by conventional karyotype analysis. Blasts of the patient failed to respond to chemotherapy and complete remission could not be attained. The abnormalities of 17p became progressively predominant in the patient. These results suggest that the blasts of a minor clone which had the abnormalities of chromosome 17p might have existed in the patient from the beginning and CMY was established from the minor clone. Investigation of p53 gene by PCR-SSCP analysis revealed that blasts of the patient showed normal patterns, while CMY showed an abnormally migrating band in exon 5 alone. This result suggests that another novel oncogenic factor(s) besides p53 might be present on chromosome 17p and other tumor suppresser genes need to be studied.
Miura
N
N
Department of Pediatrics, School of Medicine, Chiba University, Chuo-ku, Chiba 260, Japan.
Sato
T
T
Fuse
A
A
Okimoto
Y
Y
Kinugawa
N
N
Horie
H
H
Ota
S
S
Kakuda
H
H
Yokoe
H
H
Miya
T
T
Suzuki
N
N
Niimi
H
H
D002454:Cell Differentiation / Q000187:drug effects; D002869:Chromosome Aberrations; D025063:Chromosome Disorders; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D016207:Cytokines / Q000494:pharmacology; D004314:Down Syndrome / Q000235:genetics / Q000473:pathology; D016158:Genes, p53 / Q000235:genetics; D006651:Histocytochemistry; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007621:Karyotyping; D007947:Leukemia, Megakaryoblastic, Acute / Q000235:genetics* / Q000473:pathology; D008297:Male; D008854:Microscopy, Electron; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure
D016207:Cytokines