A simple, nonenzymatic method for desialylating polysialylated ganglio-N-tetraose series gangliosides to produce GM1.
Dowex-50W-H+ was used to catalyze the highly selective desialylation of polysialylated ganglio-N-tetraose series gangliosides to yield primarily GM1. High performance thin-layer chromatographic analysis of recovered lipid indicated that 60-70% of the recovered ganglioside was GM1. Identification of the major product as GM1 was confirmed by proton NMR spectra and lack of sialic acid release by Vibrio cholerae sialidase.
Schengrund
C L
CL
Department of Biochemistry and Molecular Biology, The Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Kovác
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D000818:Animals; D000837:Anion Exchange Resins; D001923:Brain Chemistry; D002417:Cattle; D002851:Chromatography, High Pressure Liquid; D005677:G(M1) Ganglioside / Q000138:chemical synthesis* / Q000737:chemistry; D005732:Gangliosides / Q000737:chemistry / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D009439:Neuraminidase; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification; D012117:Resins, Synthetic; D012794:Sialic Acids / Q000737:chemistry / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000201:enzymology
D000837:Anion Exchange Resins; D005732:Gangliosides; D009844:Oligosaccharides; D012117:Resins, Synthetic; D012794:Sialic Acids; C077982:Dowex 50; D005677:G(M1) Ganglioside; C099478:gangliotetraose; D009439:Neuraminidase