title: Intergenic sequences of clustered tRNA genes: new type of genetic marker for phylogenetic studies, with application to the taxonomy of liverworts.
abstract: We have established a new type of genetic marker in Pellia liverworts that can be used to differentiate between closely related species within this genus. The Pellia intergenic sequences spanning the tandemly repeated nuclear tRNA(Leu) genes were amplified using the PCR technique and, in the case of Pellia neesiana, the tRNA(Leu) tandem region was cloned and sequenced. The length comparison of the intergenic regions allowed for the identification of a number of Pellia species. Additional experiments suggest that this marker can be used to differentiate other species of liverworts and mosses.
authors.affiliation: Department of Gene Expression, Adam Mickiewicz University, Poznań, Poland.
authors.forename: P
authors.initials: P
authors.lastname: Fiedorow
authors.forename: Z
authors.initials: Z
authors.lastname: Szweykowska-Kulińska
mesh_terms: D001483:Base Sequence; D019068:Bryopsida / Q000145:classification* / Q000235:genetics*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics; D019143:Evolution, Molecular; D007887:Fabaceae / Q000235:genetics; D005819:Genetic Markers; D007438:Introns; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal; D016133:Polymerase Chain Reaction; D018749:RNA, Plant / Q000737:chemistry / Q000235:genetics*; D012356:RNA, Transfer, Leu / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D018749:RNA, Plant; D012356:RNA, Transfer, Leu