Synthesis of 2-deoxy-2-fluoro-phosphatidylinositol-4,5-bisphosphate and analogues: probes and modulators of the mammalian PI-PLCS.
An approach to synthesis of 2-modified phosphatidylinositol-4,5-bisphosphates, which are substrate analogues useful as probes and modulators of the PI-PLC enzyme family, is described and illustrated for the dibutyl-2-deoxy-2-fluoro analogue, a probe designed for delineating substrate and PI-PLC interactions by X-ray crystallography.
Aneja
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Functional Lipids Division, Nutrimed Biotech, Langmuir Laboratory, Ithaca, NY 14850, USA.
Ivanova
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Aneja
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D000818:Animals; D018360:Crystallography, X-Ray; D015195:Drug Design; D007202:Indicators and Reagents; D008322:Mammals; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D043265:Phosphatidylinositol Diacylglycerol-Lyase; D054801:Phosphoinositide Phospholipase C; D010738:Type C Phospholipases / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*
D007202:Indicators and Reagents; D019269:Phosphatidylinositol 4,5-Diphosphate; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; D043265:Phosphatidylinositol Diacylglycerol-Lyase