PAF antagonistic activity of 2-hydroxy-3-methoxybenzoic acid glucose ester from Gentiana scabra.
In order to find out anti-platelet activating factor (PAF) from natural resources, Korean medicinal plants used for the treatments of peripheral circulation disorders were tested for their possible protective effects on PAF-induced anaphylactic shock. From the above screening, the methanol extract of Gentiana scabra showed a potent antagonistic activity against PAF. Water suspension of the extract was partitioned with CH2Cl2 and EtOAc, successively. The EtOAc fraction which showed the highest activity was chromatographed on silica gel to yield 6 fractions. From the fraction which showed higher PAF-antagonistic activity than the other fractions, compound 1 was isolated by recrystallization. On the basis of spectral data, compound 1 was identified as 2-hydroxy-3-methoxybenzoic acid glucose ester. The compound prevented the mice from the PAF-induced death at a dose of 300 micrograms/mouse.
Huh
H
H
College of Pharmacy, Seoul National University, Korea.
Kim
H K
HK
Lee
H K
HK
D000707:Anaphylaxis / Q000517:prevention & control; D000818:Animals; D001565:Benzoates / Q000737:chemistry* / Q000302:isolation & purification* / Q000627:therapeutic use; D004353:Drug Evaluation, Preclinical; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification* / Q000627:therapeutic use; D051379:Mice; D008813:Mice, Inbred ICR; D010946:Plants, Medicinal; D010972:Platelet Activating Factor / Q000037:antagonists & inhibitors*; D011926:Rauwolfia / Q000737:chemistry*
C117314:2-hydroxy-3-methoxybenzoic acid glucose ester; D001565:Benzoates; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D010972:Platelet Activating Factor