Selection system for genes encoding nuclear-targeted proteins.
Nuclear proteins have essential roles in cell proliferation and differentiation. We have developed a yeast selection system-the nuclear transportation trap (NTT)-to identify genes encoding nuclear transport signals. Both unknown and previously identified nuclear localization signals were identified from a human fetal brain cDNA library. The majority (75%) of the unknown proteins examined were exclusively localized to the nucleus in COS-7 cells. We propose that NTT is an efficient method for isolating cDNAs that encode nuclear targeted proteins that can be applied to the retrieval of novel nuclear proteins and to annotate gene function.
Ueki
N
N
Biological Technology Laboratory, Helix Research Institute, Chiba, Japan. goose@rc.m-kagaku.co.jp
Oda
T
T
Kondo
M
M
Yano
K
K
Noguchi
T
T
Muramatsu
M
M
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000196:embryology / Q000378:metabolism; D019556:COS Cells; D002467:Cell Nucleus / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D018076:DNA, Complementary; D006801:Humans; D019913:Nuclear Localization Signals / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*
D017931:DNA Primers; D018076:DNA, Complementary; D019913:Nuclear Localization Signals; D009687:Nuclear Proteins