title: Effect in vitro of Tifell (formocresol-eugenol) on macrophage adhesion.
abstract: The purpose of this study was to investigate the effect in vitro on macrophage adhesion of Tifell (formocresoleugenol), used as an intracanal medicament and in therapeutic pulpotomies in primary teeth, Macrophages were obtained from Wistar rats. As a test of macrophage phagocytic function the adherence capacity of macrophages to a plastic surface was determined. Assays were carried out in Eppendorf tubes after 15 min of incubation at 37 degrees C in a humidified atmosphere of 5% carbon dioxide. The adherence index (AI) was calculated. Results showed that Tifell decreased significantly (P < 0.05) the AI of macrophages. Half maximal inhibition of AI was obtained at 1:334.5 Tifell dilution (AI = 27.75; P < 0.05). Taking into account that substrate adherence is the first step in the phagocytic action of macrophages and in antigen presentation, Tifell could inhibit macrophage function and modulate immune and inflammatory responses in dental pulp and periapical tissues.
authors.affiliation: Department of Dental Pathology and Therapeutics, School of Medicine and Dentistry, University of Seville, Spain.
authors.forename: J J
authors.initials: JJ
authors.lastname: Segura
authors.forename: A
authors.initials: A
authors.lastname: Jiménez-Rubio
authors.forename: J M
authors.initials: JM
authors.lastname: Guerrero
authors.forename: J R
authors.initials: JR
authors.lastname: Calvo
authors.forename: J J
authors.initials: JJ
authors.lastname: Feito
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000276:immunology; D004338:Drug Combinations; D005054:Eugenol / Q000494:pharmacology*; D005566:Formocresols / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D011672:Pulpotomy / Q000379:methods; D051381:Rats; D017208:Rats, Wistar; D012388:Root Canal Irrigants / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D005566:Formocresols; D012388:Root Canal Irrigants; C116273:Tifell; C031025:formocresol; D005054:Eugenol