title: Phosphate transporter gene family of Arabidopsis thaliana.
abstract: Using a high-affinity phosphate transporter gene of Arabidopsis thaliana, PHT1, as a probe, three Arabidopsis homologs were isolated, named PHT4, PHT5 and PHT6, in addition to the previously isolated PHT2 and PHT3. The amino acid sequences deduced from the three nucleotides were 32-42% homologous with microbial phosphate transporters of Saccharomyces cerevisiae (PHO84), Neurospora crassa (PHO-5) and Glomus versiforme (GvPT). PHT1, PHT2, PHT3 and PHT6 were clustered in a 25-kbp region of chromosome V. PHT1 and PHT4 transcripts were detected in roots. Interestingly, suspension-cultured cells expressed only PHT4. PHT4 and PHT5 located within a genetic distance of 6.4 cM on chromosome II, and were close to a phosphate accumulation mutant pho2. Genomic sequencing revealed no difference in the sequences of the two genes in both pho2 and wild-type. The PHT4 transcript was expressed at similar levels in the mutant and wild-type. These results demonstrate that neither PHT4 nor PHT5 is allelic to PHO2.
authors.affiliation: Mitsui Plant Biotechnology Research Institute, Tsukuba, Ibaraki, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okumura
authors.forename: N
authors.initials: N
authors.lastname: Mitsukawa
authors.forename: Y
authors.initials: Y
authors.lastname: Shirano
authors.forename: D
authors.initials: D
authors.lastname: Shibata
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family; D028044:Phosphate-Binding Proteins; D010710:Phosphates / Q000378:metabolism*; D028081:Proton-Phosphate Symporters; D029701:Saccharomyces cerevisiae Proteins; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D002352:Carrier Proteins; C105495:PHO84 protein, S cerevisiae; D028044:Phosphate-Binding Proteins; D010710:Phosphates; D028081:Proton-Phosphate Symporters; D029701:Saccharomyces cerevisiae Proteins