title: Co-localization of corticotropin-releasing hormone with glutamate decarboxylase and calcium-binding proteins in infant rat neocortical interneurons.
abstract: Corticotropin releasing hormone (CRH) has been localized to interneurons of the mammalian cerebral cortex, but these neurons have not been fully characterized. The present study determined the extent of co-localization of CRH with glutamate decarboxylase (GAD) and calcium-binding proteins in the infant rat neocortex using immunocytochemistry. CRH-immunoreactive (ir) neurons were classified into two major groups. The first group was larger and consisted of densely CRH-immunostained small bipolar cells, predominantly localized to layers II and III. The second group of CRH-ir cells was lightly labeled and included multipolar neurons mainly found in deep cortical layers. Co-localization studies indicated that the vast majority of CRH-ir neurons, including both bipolar and multipolar types, was co-immunolabeled for GAD-65 and GAD-67. Most multipolar, but only some bipolar, CRH-ir neurons also contained parvalbumin, while CRH-ir neurons rarely contained calbindin or calretinin. These results indicate that virtually all CRH-ir neurons in the rat cerebral cortex are GABAergic. Furthermore, since parvalbumin is expressed by cortical basket and chandelier cells, the co-localization of CRH and parvalbumin suggests that some cortical CRH-ir neurons may belong to these two cell types.
authors.affiliation: Department of Anatomy and Neurobiology, University of California at Irvine, 92697, USA.
authors.forename: X X
authors.initials: XX
authors.lastname: Yan
authors.forename: T Z
authors.initials: TZ
authors.lastname: Baram
authors.forename: A
authors.initials: A
authors.lastname: Gerth
authors.forename: L
authors.initials: L
authors.lastname: Schultz
authors.forename: C E
authors.initials: CE
authors.lastname: Ribak
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins / Q000032:analysis*; D003346:Corticotropin-Releasing Hormone / Q000032:analysis*; D005968:Glutamate Decarboxylase / Q000032:analysis*; D007150:Immunohistochemistry; D007395:Interneurons / Q000737:chemistry*; D019579:Neocortex / Q000737:chemistry* / Q000166:cytology; D009419:Nerve Tissue Proteins / Q000032:analysis*; D010320:Parvalbumins / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000032:analysis
chemical_list: C576512:Calb2 protein, rat; D064032:Calbindin 2; D064026:Calbindins; D002135:Calcium-Binding Proteins; D009419:Nerve Tissue Proteins; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D003346:Corticotropin-Releasing Hormone; D005968:Glutamate Decarboxylase