[The effect of wuto granules on the levels of excitatory amino acids in extracellullar fluid of rat hippocampus following cerebral ischemia].
We observed the changes of excitatory amino acids (EAA) in extracellular fluid (ECLF) in rat hippocampus in case of cerebral ischemia after giving Wuto Granule compring the test groups with the control groups by microdialysis method. Results showed that there was no effect on EAA in extrcellular fluid by the drug before cerebral ischemia, but there existed staistical singnifcant changes of EAA in extracellular fluid one hour after cerebral ischemia comparing the test groups Glu and Asp with the control groups (P < 0.01). We found that there is a decrease of release of release of EAA in ECLF in case of ischemia by Wuto Granule. It could be deduced that Wuto Granule can penetrate the brain-blood barrier and this can protect the brain incse of ischemia. The application of intracerebral micrdialysis has provided a new method for the research work of pharmacology.
Li
J
J
Institute of Psychiatry, Xiangya Hospital, Hunan Medical University, Shangsha.
Yang
Q
Q
Hu
S
S
D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D001812:Blood-Brain Barrier; D002545:Brain Ischemia / Q000378:metabolism* / Q000517:prevention & control; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D018846:Excitatory Amino Acids / Q000378:metabolism*; D005110:Extracellular Space / Q000378:metabolism; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017208:Rats, Wistar
D004365:Drugs, Chinese Herbal; D018846:Excitatory Amino Acids; D001224:Aspartic Acid; D018698:Glutamic Acid