Specific gravity of Opisthorchis viverrini eggs.
The specific gravity of the eggs of the liver fluke Opisthorchisviverrini was determined using a sucrose gradient centrifugation and found to range from 1.2713 to 1.3043. The peak egg count was located at the sucrose fraction with a specific gravity of 1.2814. An attempt to float eggs in saturated sodium nitrate solution, sp.gr. 1.4, failed. Examination of human stool specimens for Oviverrini eggs by simple flotation in saturated sodium nitrate solution and the formol-ether sedimentation technique revealed that the flotation technique was not as efficient as the sedimentation technique. It was suggested that the flotation techniques were inappropriate for the detection of Oviverrini eggs in faeces or contaminated soil.
Harnnoi
T
T
Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. tharnnoi@suandok01.medicine.cmu.ac.th
Wijit
A
A
Morakote
N
N
Pipitgool
V
V
Maleewong
W
W
D000818:Animals; D002499:Centrifugation, Density Gradient; D005243:Feces / Q000469:parasitology*; D006801:Humans; D009891:Opisthorchis / Q000302:isolation & purification*; D010063:Ovum; D010270:Parasite Egg Count / Q000379:methods*; D013046:Specific Gravity