Apoptosis induction of POS canine osteosarcoma cells by vitamin D and retinoids.
Vitamin D3: 1-alpha, 25(OH)2D3 (calcitriol), 22-oxa-1,25(OH)2D3 (OCT), cholecalciferol (vitamin D3), and retinoids: all-trans retinoic acid (ATRA) and 9-cis retinoic acid, induced morphological changes in POS canine osteosarcoma cells into elongated, spindle or fibroblast like-shaped cells, and apoptotic like cell death characterized by cell shrinkage, condensation and margination of the nucleus for all drugs at 10(-6)M-10(-9)M after 72 to 120 hr culture. Apoptosis as shown by DNA laddering was induced at 48 hr by all drugs at 10(-6)M, 10(-7)M at 96 hr, 10(-8)M and 10(-9)M at 120 hr respectively. These vitamins are suggested to adjunct antineoplastic agents in canine osteosarcoma therapy by induction of apoptosis.
Barroga
E F
EF
Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Kadosawa
T
T
Asano
K
K
Okumura
M
M
Fujinaga
T
T
D000818:Animals; D017209:Apoptosis; D001859:Bone Neoplasms / Q000473:pathology / Q000662:veterinary*; D002117:Calcitriol / Q000494:pharmacology; D002762:Cholecalciferol / Q000494:pharmacology*; D004283:Dog Diseases / Q000473:pathology*; D004285:Dogs; D004587:Electrophoresis, Agar Gel; D012516:Osteosarcoma / Q000473:pathology / Q000662:veterinary*; D012176:Retinoids / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D012176:Retinoids; D002762:Cholecalciferol; D002117:Calcitriol