Effect of interleukin-12 and interleukin-10 on the virus replication and apoptosis in T-cells infected with feline immunodeficiency virus.
Interleukin-12 (IL-12) is an important cytokine for Th1 response which stimulates the T-cell population to produce cytokines for cellular immunity. Interleukin-10 (IL-10) is a pleiotropic cytokine capable of suppressing cytokine production from macrophages and T-cells and participants in Th2 immune response. The present study was carried out to examine the effect of these cytokines on virus replication and apoptosis in T-cells infected with feline immunodeficiency virus (FIV). Infection of a feline T-lymphoid cell line (Fel-039) resulted in an increase of the reverse transcriptase (RT) activity in the culture supernatant accompanied by cell death from apoptosis. Addition of human recombinant IL-12 significantly inhibited the virus replication and apoptosis in Fel-039 cells in a dose dependent manner. Furthermore, the antiviral activity of IL-12 was associated with the expression of IFN-gamma in the FIV-infected Fel-039 cells. In contrast, human recombinant IL-10 did not show any inhibitory effect on the virus replication and apoptosis in the Fel-039 cells infected with FIV. These results suggest that the inhibitory effect of IL-12 on both virus replication and apoptosis has potential implications for the design of immunotherapy strategies using IL-12 in FIV infection.
Mortola
E
E
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Endo
Y
Y
Mizuno
T
T
Ohno
K
K
Watari
T
T
Tsujimoto
H
H
Hasegawa
A
A
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002415:Cats; D002460:Cell Line; D006801:Humans; D016089:Immunodeficiency Virus, Feline / Q000502:physiology*; D016753:Interleukin-10 / Q000494:pharmacology*; D018664:Interleukin-12 / Q000494:pharmacology*; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D011994:Recombinant Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects*
D000276:Adjuvants, Immunologic; D011994:Recombinant Proteins; D016753:Interleukin-10; D018664:Interleukin-12