title: A natural variant of the cysteine protease virulence factor of group A Streptococcus with an arginine-glycine-aspartic acid (RGD) motif preferentially binds human integrins alphavbeta3 and alphaIIbbeta3.
abstract: The human pathogenic bacterium group A Streptococcus produces an extracellular cysteine protease [streptococcal pyrogenic exotoxin B (SpeB)] that is a critical virulence factor for invasive disease episodes. Sequence analysis of the speB gene from 200 group A Streptococcus isolates collected worldwide identified three main mature SpeB (mSpeB) variants. One of these variants (mSpeB2) contains an Arg-Gly-Asp (RGD) sequence, a tripeptide motif that is commonly recognized by integrin receptors. mSpeB2 is made by all isolates of the unusually virulent serotype M1 and several other geographically widespread clones that frequently cause invasive infections. Only the mSpeB2 variant bound to transfected cells expressing integrin alphavbeta3 (also known as the vitronectin receptor) or alphaIIbbeta3 (platelet glycoprotein IIb-IIIa), and binding was blocked by a mAb that recognizes the streptococcal protease RGD motif region. In addition, mSpeB2 bound purified platelet integrin alphaIIbbeta3. Defined beta3 mutants that are altered for fibrinogen binding were defective for SpeB binding. Synthetic peptides with the mSpeB2 RGD motif, but not the RSD sequence present in other mSpeB variants, blocked binding of mSpeB2 to transfected cells expressing alphavbeta3 and caused detachment of cultured human umbilical vein endothelial cells. The results (i) identify a Gram-positive virulence factor that directly binds integrins, (ii) identify naturally occurring variants of a documented Gram-positive virulence factor with biomedically relevant differences in their interactions with host cells, and (iii) add to the theme that subtle natural variation in microbial virulence factor structure alters the character of host-pathogen interactions.
authors.affiliation: Institute for the Study of Human Bacterial Pathogenesis, Department of Pathology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Stockbauer
authors.forename: L
authors.initials: L
authors.lastname: Magoun
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.forename: E H
authors.initials: EH
authors.lastname: Burns
authors.forename: S
authors.initials: S
authors.lastname: Gubba
authors.forename: S
authors.initials: S
authors.lastname: Renish
authors.forename: X
authors.initials: X
authors.lastname: Pan
authors.forename: S C
authors.initials: SC
authors.lastname: Bodary
authors.forename: E
authors.initials: E
authors.lastname: Baker
authors.forename: J
authors.initials: J
authors.lastname: Coburn
authors.forename: J M
authors.initials: JM
authors.lastname: Leong
authors.forename: J M
authors.initials: JM
authors.lastname: Musser
mesh_terms: D000483:Alleles; D000818:Animals; D001426:Bacterial Proteins; D016466:CHO Cells; D002448:Cell Adhesion / Q000187:drug effects; D006224:Cricetinae; D003546:Cysteine Endopeptidases / Q000235:genetics* / Q000378:metabolism*; D004730:Endothelium, Vascular / Q000166:cytology; D014644:Genetic Variation; D006801:Humans; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D009842:Oligopeptides / Q000235:genetics / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D019030:Receptors, Vitronectin / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins; D013297:Streptococcus pyogenes / Q000201:enzymology / Q000472:pathogenicity*
chemical_list: D001426:Bacterial Proteins; D016023:Integrins; D009842:Oligopeptides; D010446:Peptide Fragments; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D019030:Receptors, Vitronectin; D011994:Recombinant Proteins; C047981:arginyl-glycyl-aspartic acid; D003546:Cysteine Endopeptidases; C042477:streptopain