Determination of betamethasone dipropionate and salicylic acid in pharmaceutical preparations by high-performance liquid chromatography.
The simultaneous determination of betamethasone dipropionate (BD) and salicylic acid (SA) in both ointment and topical solution was developed using high-performance liquid chromatography (HPLC). The method was standardized using a LiChrospher 100 RP-18 (125 x 4 mm, 5 microns) column, acetonitrile-tetrahydrofuran-acetic acid 1% (25:20:55 v/v), apparent pH 3.3, as mobile phase, and UV detection at 254 nm. The peak area response versus concentration was linear in a concentration range from 5.0 to 50.0 micrograms/ml of BD and from 20.0 to 200.0 micrograms/ml of SA. The correlation coefficients were 0.9997 for BD and 0.9987 for SA, and the relative standard errors of estimates were 1.38% for BD and 3.27% for SA. The coefficient of variation and the recovery average were, respectively, 0.41-1.15% and 100.09% for BD, and 0.57-0.95% and 99.79% for SA.
Kedor-Hackmann
E R
ER
Faculty of Pharmaceutical Sciences, University of São Paulo, Brazil.
Gianotto
E A
EA
Santoro
M I
MI
D000287:Administration, Topical; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000032:analysis*; D001623:Betamethasone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000032:analysis; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D005938:Glucocorticoids; D006801:Humans; D009824:Ointments; D020156:Salicylic Acid / Q000008:administration & dosage / Q000032:analysis*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D000893:Anti-Inflammatory Agents; D005938:Glucocorticoids; D009824:Ointments; D012996:Solutions; C011175:betamethasone-17,21-dipropionate; D001623:Betamethasone; D020156:Salicylic Acid