Antibodies against type II phospholipase A2 prevent renal injury due to ischemia and reperfusion in rats.
This study was performed to determine the involvement of type II phospholipase A2 (PLA2-II) in renal injury caused by ischemia and reperfusion. Ischemia and reperfusion significantly elevated levels of blood urea nitrogen and serum creatinine in rats. These increases were significantly reduced by i.v. administration of rabbit IgG F(ab')2 fragments against rat PLA2-II. Increased levels of acid-stable PLA2 activity in the kidney were caused by ischemia and reperfusion, and were suppressed by administration of anti-PLA2-II F(ab')2. Increased levels of myeloperoxidase activity, a marker of neutrophil infiltration, in the kidney were also reduced after anti-PLA2-II F(ab')2 treatment. These results suggest that PLA2-II plays a pivotal role in pathogenesis of ischemia and reperfusion injury through induction of neutrophil infiltration.
Takasaki
J
J
Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co. Ltd., Tsukuba, Ibaraki, Japan. takasaki@yamanouchi.co.jp
Kawauchi
Y
Y
Urasaki
T
T
Tanaka
H
H
Usuda
S
S
Masuho
Y
Y
D058186:Acute Kidney Injury / Q000201:enzymology / Q000503:physiopathology; D000818:Animals; D000918:Antibody Specificity; D054501:Group II Phospholipases A2; D007140:Immunoglobulin Fab Fragments / Q000276:immunology / Q000627:therapeutic use*; D007668:Kidney / Q000201:enzymology / Q000503:physiopathology; D008297:Male; D009195:Peroxidase / Q000378:metabolism; D010741:Phospholipases A / Q000276:immunology* / Q000378:metabolism; D054467:Phospholipases A2; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000201:enzymology / Q000276:immunology / Q000517:prevention & control*
D007140:Immunoglobulin Fab Fragments; D009195:Peroxidase; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2