A novel Bacillus subtilis gene, antE, temporally regulated and convergent to and overlapping dnaE.
A Bacillus subtilis promoter, Px, that functions in a convergent manner with the sigA operon promoter P3 has been found in the sigA operon. Promoter Px is turned on at the same time as promoter P3 during early sporulation. The transcript from promoter Px codes for a small protein with partial homology to the OmpR protein from Escherichia coli and also carries an untranslated sequence at its 3' end that is complementary to the 5' end of the P3 transcript, which codes for the ribosome binding site of dnaE. The gene controlled by Px has been called antE. The expression of antE does not require sigmaB, sigmaE, or sigmaH. Px was transcribed in vitro by the sigmaA holoenzyme and is the seventh promoter to be recognized in the sigmaA operon. A possible role for the antE gene during early sporulation is proposed.
Wang
L F
LF
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA.
Park
S S
SS
Doi
R H
RH
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D004258:DNA Polymerase III / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D005807:Genes, Overlapping; D007763:Lac Operon; D008969:Molecular Sequence Data; D009876:Operon; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012808:Sigma Factor / Q000235:genetics; D013171:Spores, Bacterial / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics
C116901:AntE protein, Bacillus subtilis; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D012808:Sigma Factor; D015534:Trans-Activators; C112177:osmolarity response regulator proteins; C074904:DNA polymerase III, alpha subunit; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D004258:DNA Polymerase III