Biological activities of a novel lectin derived from silkworm faeces: characteristic changes of mouse peritoneal macrophages by the lectin.
A novel lectin derived from silkworm faeces, named NUE, activates phagocytosis of mouse peritoneal macrophages. At this time, significant morphological changes of the cells take place. NUE-treated macrophages formed projection-like neurocytes within 12 hr of treatment, and appeared somewhat flat in shape with the activation of cell growth. Associated with the morphological changes, actin was organized in dot-like structures corresponding to cell-substratum contact sites in NUE-treated macrophages. Vinculin, a cytoskeletal protein involved in microfilament-membrane interaction, formed doughnut-like rings matching the actin-dots, called "podosomes". Furthermore, observation by interference reflection microscopy showed that NUE-treated macrophages adhered more strongly to the substratum at local areas. According to these changes, more proteins associated with cell-substratum contact sites became to detergent-resistant. It was shown that NUE changed adhesive form of mouse peritoneal macrophages structurally and qualitatively.
Hirayama
E
E
Institutes of Molecular and Cellular Biology for Pharmaceutical Sciences, Kyoto Pharmaceutical University, Japan.
Maekawa
H
H
Hiraki
A
A
Kim
J
J
D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003902:Detergents / Q000494:pharmacology; D005243:Feces / Q000737:chemistry; D015151:Immunoblotting; D037102:Lectins / Q000096:biosynthesis / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D051379:Mice; D008856:Microscopy, Fluorescence; D008857:Microscopy, Interference; D017830:Octoxynol / Q000494:pharmacology
D003902:Detergents; D037102:Lectins; C117539:NUE protein, silkworm; D017830:Octoxynol