title: Enzymatic alpha(2-3)sialylation of non-natural type-I (Lewisc) disaccharides with recombinant sialyl-transferase.
abstract: Recombinant alpha(2-3)sialyl-transferase from rat liver is used to sialylate a series of type-I (Lewisc) disaccharides on a preparative scale. The enzyme tolerates a broad array of N-acetyl replacements of the N-glucosamine subunit ranging from small and large lipophilic groups to charged and heterocyclic amides.
authors.affiliation: Novartis Pharma AG, Basle, Switzerland.
authors.forename: G
authors.initials: G
authors.lastname: Baisch
authors.forename: R
authors.initials: R
authors.lastname: Ohrlein
authors.forename: M
authors.initials: M
authors.lastname: Streiff
mesh_terms: D000818:Animals; D002240:Carbohydrate Sequence; D004187:Disaccharides / Q000737:chemistry*; D008099:Liver / Q000201:enzymology; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D012799:Sialyltransferases / Q000737:chemistry*
chemical_list: D004187:Disaccharides; D011994:Recombinant Proteins; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid