title: Use of DNA fingerprinting to assess tuberculosis infection control.
abstract: BACKGROUND
  DNA fingerprinting establishes the genetic relatedness of Mycobacterium tuberculosis isolates and has become a powerful tool in tuberculosis epidemiology.
  
  
  OBJECTIVE
  To use DNA fingerprinting to assess the efficacy of current tuberculosis infection-control practices.
  
  
  METHODS
  Retrospective molecular and descriptive epidemiologic study.
  
  
  METHODS
  A 700-bed urban public hospital that follows the Centers for Disease Control and Prevention (CDC) guidelines for tuberculosis infection control.
  
  
  METHODS
  183 patients who had positive cultures for M. tuberculosis from 1 April 1995 to 31 March 1996.
  
  
  RESULTS
  173 of 183 M. tuberculosis isolates from the study period underwent DNA fingerprinting. Fingerprinting revealed that five isolates represented false-positive cultures and that 91 (54%) of the remaining 168 isolates were in 15 DNA fingerprinting clusters, which ranged in size from 2 to 29 isolates. Risk factors for clustering were birth in the United States, African-American ethnicity, homelessness, substance abuse, and male sex. Retrospective epidemiologic analysis of inpatient and outpatient visits by the 91 patients who had clustered isolates revealed only one possible instance of patient-to-patient transmission.
  
  
  CONCLUSIONS
  The DNA fingerprinting of all M. tuberculosis isolates from a 1-year period revealed one possible instance of nosocomial transmission and five false-positive M. tuberculosis cultures. However, these results did not lead to changes in infection-control practices or in clinical care. The study findings do not support the use of DNA fingerprinting for nosocomial tuberculosis surveillance, but they suggest that compliance with the CDC tuberculosis infection-control guidelines may control patient-to-patient transmission in high-risk urban hospitals.
authors.affiliation: Cook County Hospital, Rush Medical College, Chicago, Illinois 60612, USA.
authors.forename: A L
authors.initials: AL
authors.lastname: French
authors.forename: S F
authors.initials: SF
authors.lastname: Welbel
authors.forename: S E
authors.initials: SE
authors.lastname: Dietrich
authors.forename: L B
authors.initials: LB
authors.lastname: Mosher
authors.forename: P S
authors.initials: PS
authors.lastname: Breall
authors.forename: W S
authors.initials: WS
authors.lastname: Paul
authors.forename: F E
authors.initials: FE
authors.lastname: Kocka
authors.forename: R A
authors.initials: RA
authors.lastname: Weinstein
mesh_terms: D002641:Chicago; D016000:Cluster Analysis; D016358:Contact Tracing; D003428:Cross Infection / Q000382:microbiology / Q000517:prevention & control* / Q000635:transmission; D016172:DNA Fingerprinting; D005189:False Positive Reactions; D005260:Female; D006786:Hospitals, Urban; D006801:Humans; D017053:Infection Control / Q000379:methods*; D008297:Male; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012189:Retrospective Studies; D012307:Risk Factors; D013223:Statistics as Topic; D014374:Tuberculin Test; D014397:Tuberculosis, Pulmonary / Q000382:microbiology / Q000517:prevention & control* / Q000635:transmission