Isolation and characterization of a new transposable element in Chlamydomonas reinhardtii.
A new transposable element, Tcr3, was identified in the unicellular green alga Chlamydomonas reinhardtii. The Tcr3 element contained imperfect terminal inverted repeat sequences of 56 bp and created a 2 bp target site duplication upon insertion. Insertion of Tcr3 into the 3'-untranslated region of the NIT8 gene, which is essential for nitrate assimilation, prevented expression of the gene. Excision of the Tcr3 element correlated with reversion of the mutant phenotype and left behind a 3 bp footprint. Tcr3 was found in all Chlamydomonas isolates tested and should prove to be useful for transposon-tagging experiments in Chlamydomonas.
Wang
S C
SC
Plant Molecular Genetics Institute, Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108, USA.
Schnell
R A
RA
Lefebvre
P A
PA
D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002352:Carrier Proteins / Q000235:genetics; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D004251:DNA Transposable Elements / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics; D015800:Protozoan Proteins; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D002352:Carrier Proteins; D004251:DNA Transposable Elements; D047908:Intracellular Signaling Peptides and Proteins; C095726:NIT8 protein, Chlamydomonas reinhardtii; D009687:Nuclear Proteins; D015800:Protozoan Proteins; D004247:DNA