Platelets stored in a new-generation container differences between plasma and platelet additive solution II.
OBJECTIVE
In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelets in plasma and platelets additive solution.


METHODS
Platelet concentrate pools (PCs) of different yields suspended in either plasma (PCs-PL; n = 30) or PAS II (PCs-PAS; n = 37) were prepared. For preparation of PCs with a low, intermediate and high number of platelets 3, 5 and 6 buffy coat (BCs) were pooled with different volumes of plasma and 5 and 6 BCs were pooled with different volumes of PAS II, in order to obtain PCs of equal volumes comparable with routine conditions. All PCs were stored on a flatbed shaker at 22+/-2 degrees C and evaluated on days 1, 2, 5 and 7 by determining platelet and white cell counts, pH (37 degrees C), pO2, pCO2 and swirling score.


RESULTS
Platelet yields ranged from 1.5 up to 5.5 x 10(11) platelets/U. On day 7 all PCs-PL (n = 4) with platelet yields above 4.5 x 10(11)/U had a pH value below 6.8 (range 5.91-6.79). While 7 of 8 PCs-PAS units with platelet yields above 4. 0 x 10(11)/U showed a pH value below 6.8 (range 6.31-6.70).


CONCLUSIONS
During 7 days of storage in a new 1.3-liter platelet container, the pH was maintained above 6.8 in PCs in plasma with a yield between 1.5- and 4.5 x 10(11)/U; when PAS II was used, the maximum platelet yield allowed for comparable pH maintenance was somewhat lower (4.0 x 10(11)/U).
de Wildt-Eggen
J
J
Red Cross Bloodbank IJssellanden, Zwolle, The Netherlands.
Schrijver
J G
JG
Smid
W M
WM
Joie
M
M
Bollinne
V
V
Bins
M
M
D000328:Adult; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism; D001793:Blood Preservation / Q000295:instrumentation* / Q000379:methods; D002021:Buffers; D002245:Carbon Dioxide / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000032:analysis; D010313:Partial Pressure; D010539:Permeability; D010949:Plasma; D015539:Platelet Activation; D010976:Platelet Count; D011090:Polyenes / Q000737:chemistry; D013499:Surface Properties
D002021:Buffers; D011090:Polyenes; D002245:Carbon Dioxide; C035051:PL 732; D010100:Oxygen