[Occurrence of extended spectrum beta-lactamases (ESBL) and inducible beta-lactamases (IBL) in clinical strains of gram-negative rods].
This study was undertaken to check the situation concerning the occurrence of Gram-negative rods producing extended-spectrum beta-lactamases (ESBL) and inducible beta-lactamases (IBL) in clinical specimens from patients hospitalized in National Clinical Hospital No. 1 in Warsaw. Such determinations were not performed in this hospital so far. During three months (April-June, 1997) 200 strains of Gram-negative rods were cultured. The strains were identified in automatic ATB system using strips with biochemical tests: ID 32 E for enteric rods and ID 32 GN for non-fermenting rods. ESBL-producing strains were detected with double disc diffusion test according to Jarlier et al. (1988). Clavulanate was applied as the inhibitor of beta-lactamases (AMO/CLAV disc). Inducible beta-lactamases were determined using double disc method according to Sanders and Sanders (1979). Cefoxitin was the inductor of these beta-lactamases. 82 strains (41% of all strains) belonging to Enterobacteriaceae family, 92 strains (46%) of Pseudomonadaceae rods and 26 strains (13%) of other Gram-negative rods were isolated. 30 ESBL-producing strains (15% of all strains) and 45 strains (22.5%) with IBL activity were detected. The obtained results confirm the necessity of continuous and reliable monitoring of ESBL--and IBL--producing strains among Gram-negative rods isolated from clinical materials. The aims of such procedure are the control and prevention of their dissemination within a hospital as well as the avoidance of therapeutic failures.
Rokosz
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Zakład Bakteriologii Klinicznej AM w Warszawie.
Sawicka-Grzelak
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D019818:Clavulanic Acid / Q000494:pharmacology; D003428:Cross Infection / Q000382:microbiology / Q000517:prevention & control; D004755:Enterobacteriaceae / Q000145:classification / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D011548:Pseudomonadaceae / Q000145:classification / Q000201:enzymology; D013045:Species Specificity; D001618:beta-Lactamases / Q000096:biosynthesis*
D004791:Enzyme Inhibitors; D019818:Clavulanic Acid; D001618:beta-Lactamases
Wystepowanie beta-laktamaz o rozszerzonym spektrum substratowym (ESBL) i beta-laktamaz indukowanych (IBL) u klinicznych szczepów pałeczek Gram-ujemnych.