Functionalization of a viscosity-sensitive fluorophore for probing of biological systems.
Functionalization of a viscosity-sensitive visible wavelength fluorophore 2-(1,1-dicyanopropenyl-2)-6-dimethylaminonaphthalene (DDNP), with the intent to incorporate its favorable optical properties into a probe for structural and functional imaging by fluorescence microscopy, is described. Spiperone, a highly potent ligand for the dopamine D2 receptors, was conjugated via an ethylpiperazine moiety to the fluorophore giving fluorescent probes that can be excited in the UV and Vis range.
Petric
A
A
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia. andrej.petric@uni-lj.si
Jacobson
A F
AF
Barrio
J R
JR
D015081:2-Naphthylamine / Q000031:analogs & derivatives* / Q000737:chemistry; D000181:Acrylonitrile / Q000031:analogs & derivatives* / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D011869:Radioligand Assay; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D011985:Receptors, Serotonin / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013134:Spiperone / Q000378:metabolism
C116723:2-(1,1-dicyanopropenyl-2-)-6-dimethylaminonaphthaleney; D005456:Fluorescent Dyes; D017448:Receptors, Dopamine D2; D011985:Receptors, Serotonin; D013134:Spiperone; D015081:2-Naphthylamine; D000181:Acrylonitrile