Organometallic photonucleases: a novel class of DNA-cleaving agents.
The first demonstration of DNA cleavage by an organic radical generated via homolysis of a metal-alkyl bond in a Cp-metal complex is presented. Irradiation of CpW(CO)3CH3 (1.5 molecules/base pair) produced methyl radical, giving single-strand cleavage of pBR322 DNA. This process was inhibited by the general radical trap cysteine and by TEMPO, which traps carbon radicals but not oxygen-centered radicals.
Mohler
D L
DL
Department of Chemistry, West Virginia University, Morgantown 26506-6045, USA.
Dain
D R
DR
Kerekes
A D
AD
Nadler
W R
WR
Scott
T L
TL
D003497:Cyclic N-Oxides; D003545:Cysteine; D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases; D005609:Free Radicals; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D010782:Photolysis; D010957:Plasmids / Q000737:chemistry*
D003497:Cyclic N-Oxides; D005609:Free Radicals; D009942:Organometallic Compounds; D004247:DNA; D003851:Deoxyribonucleases; D003545:Cysteine; C003959:TEMPO