Phylogenetic analysis of an anaerobic, trichlorobenzene-transforming microbial consortium.
A culture-independent phylogenetic survey for an anaerobic trichlorobenzene-transforming microbial community was carried out. Small-subunit rRNA genes were PCR amplified from community DNA by using primers specific for Bacteria or Euryarchaeota and were subsequently cloned. Application of a new hybridization-based screening approach revealed 51 bacterial clone families, one of which was closely related to dechlorinating Dehalobacter species. Several clone sequences clustered to rDNA sequences obtained from a molecular study of an anaerobic aquifer contaminated with hydrocarbons and chlorinated solvents (Dojka et al., Appl. Env. Microbiol. 64:3869-3877, 1998).
von Wintzingerode
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Institut für Mikrobiologie und Hygiene, Universitätsklinikum Charité, 10117 Berlin, Germany.
Selent
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Hegemann
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Göbel
U B
UB
D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D001483:Base Sequence; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002722:Chlorobenzenes / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction
D002722:Chlorobenzenes; D017931:DNA Primers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; C036720:trichlorobenzene