Albumin-binding surfaces: in vitro activity.
Immobilized monoclonal antibodies (Mabs) have been used to attract specific molecules to a solid surface from complex mixtures such as blood, plasma or serum, thereby directing the response to the modified substrate, a key goal in rational biomaterial design. The nature of the Mab dictated the nature of the response: anti-albumin antibodies were used to prevent cell and platelet adhesion in vitro, whilst anti-fibronectin Mabs promoted attachment. Patterned surfaces could be formed, bearing Mabs that generated adhesive and non-adhesive regions. Fibrinogen adsorption from plasma showed a Vroman peak on unmodified control polymer, which was reduced by 64% in the presence of surface-bound anti-albumin Mab. Immobilization of a control Mab reduced fibrinogen adsorption only slightly, implying an albumin-mediated effect. In static tests, platelet adhesion from human platelet rich plasma was significantly reduced by the immobilization of anti-HSA Mab when compared to the untreated FEP surface (p < 0.0001). This effect was also seen with citrated blood flowing through Mab-treated polyurethane tubing at a shear rate of 132 s(-1) (p=0.034). Since platelets and proteins (as blood, plasma or serum) were introduced to the surface simultaneously, the generation of a defined protein film must have been sufficiently rapid as to shape the platelet or cell response.
McFarland
C D
CD
Cooperative Research Centre for Cardiac Technology, CSIRO Molecular Science, Sydney Laboratory, North Ryde, NSW, Australia.
De Filippis
C
C
Jenkins
M
M
Tunstell
A
A
Rhodes
N P
NP
Williams
D F
DF
Steele
J G
JG
D000911:Antibodies, Monoclonal / Q000378:metabolism*; D001792:Blood Platelets / Q000166:cytology* / Q000378:metabolism; D002448:Cell Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism / Q000493:pharmacokinetics*; D005353:Fibronectins / Q000276:immunology; D006801:Humans; D011138:Polytetrafluoroethylene / Q000737:chemistry; D012709:Serum Albumin / Q000276:immunology / Q000378:metabolism*; D013499:Surface Properties
D000911:Antibodies, Monoclonal; D020099:Coated Materials, Biocompatible; D005353:Fibronectins; D012709:Serum Albumin; D005340:Fibrinogen; D011138:Polytetrafluoroethylene