title: Terminal deoxynucleotidyl transferase expression during neonatal life alters D(H) reading frame usage and Ig-receptor-dependent selection of V regions.
abstract: During neonatal life, Ig diversity is limited in many respects. The absence of terminal deoxynucleotidyl transferase (TdT) expression with the consequent lack of nontemplated addition during the neonatal period, coupled with the predominant usage of a single D(H) reading frame (RF), leads to severe limitations of diversity in the CDR3 region of Ig heavy (H) chains. The neonatal Ig H chain repertoire is also characterized by restricted V(H) usage, with predominant expression of certain V(H) segments, such as V(H)81x, that are rarely evident during adult life. In this report, we examine the effect of enforced TdT expression on the neonatal repertoire of V(H)81xDJ(H) rearrangements. We find that TdT synthesis abrogates D(H) RF bias during the fetal/neonatal period through a Ig-receptor-independent mechanism. These findings suggest that D(H) RF bias during neonatal life is determined largely by homology-directed joining. We also find that TdT synthesis alters the selection of productively rearranged V(H)81xDJ(H) alleles in the neonatal spleen through a Ig-receptor-dependent mechanism. Analysis of predicted CDR3 amino acid sequences indicates that positive selection of V(H)81x-encoded H chains is correlated with the presence of a consensus sequence immediately adjacent to the V(H) segment. These data support the hypothesis that the CDR3 region is critical in determining the ability of V(H)81x-encoded H chains to form functional receptors that support positive selection of B lymphocytes. Together, our results demonstrate that TdT can indirectly influence the Ig repertoire by influencing both receptor-dependent and receptor-independent selection processes.
authors.affiliation: Ontario Cancer Institute and Department of Immunology, University of Toronto, Ontario, Canada.
authors.forename: A J
authors.initials: AJ
authors.lastname: Marshall
authors.forename: N
authors.initials: N
authors.lastname: Doyen
authors.forename: L A
authors.initials: LA
authors.lastname: Bentolila
authors.forename: C J
authors.initials: CJ
authors.lastname: Paige
authors.forename: G E
authors.initials: GE
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000831:Animals, Newborn; D000916:Antibody Diversity; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D017637:Clonal Deletion; D016384:Consensus Sequence; D004253:DNA Nucleotidylexotransferase / Q000096:biosynthesis* / Q000235:genetics; D004790:Enzyme Induction; D016694:Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; D005803:Genes, Immunoglobulin; D007107:Immune System / Q000196:embryology* / Q000254:growth & development; D007138:Immunoglobulin delta-Chains / Q000235:genetics*; D051379:Mice; D008822:Mice, Transgenic; D018448:Models, Immunological; D008969:Molecular Sequence Data; D016366:Open Reading Frames
chemical_list: D007138:Immunoglobulin delta-Chains; D004253:DNA Nucleotidylexotransferase