Macrolide biosynthesis: a single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in Streptomyces venezuelae.
The final step in the biosynthesis of methymycin, neomethymycin, and picromycin is an hydroxylation, shown to be carried out by the cytochrome P-450 monooxygenase, PicK. Direct comparison of the relative Kcat/K(m) values for the two substrates, YC-17 and narbomycin, showed a threefold rate preference of picK for narbomycin.
Graziani
E I
EI
Department of Chemistry, Brown University, Providence, RI 02912-9108, USA.
Cane
D E
DE
Betlach
M C
MC
Kealey
J T
JT
McDaniel
R
R
D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D006900:Hydroxylation; D018942:Macrolides; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; C008517:methymycin; C008594:neomethymycin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C115353:PicK protein, Streptomyces venezuelae; C003145:picromycin