Effect of tenascin-C deficiency on chemically induced dermatitis in the mouse.
Tenascin-C is a large extracellular matrix glycoprotein characterized by its spatiotemporal expression during embryogenesis, carcinogenesis, and wound healing. Many in vitro studies on tenascin-C have revealed its multifunctional properties; however, disruption of the tenascin-C gene did not reveal any obvious abnormalities during development, and its function in vivo remains unclear. Here, we investigated whether tenascin-C is involved in inflammatory dermatitis in adults by studying chemically induced dermatitis in tenascin-C knockout mice. An epicutaneous application of a hapten, 2,4-dinitrofluorobenzene, to the ear skin of BALB/CA mice resulted in inflammation and induced the expression of tenascin-C. In congenic tenascin-C knockout mice, the dermatitis occurred more severely than in wild-type mice; infiltration of polymorphonuclear cells in knockout mice persisted longer than in wild-type mice, and the elastosis-like disorganized extracellular matrix was also seen in the ear. These results suggest that tenascin-C plays a role in vivo in inflammatory responses in the skin, and that the genetic background has profound effects on the function of tenascin-C in mouse dermatitis.
Koyama
Y
Y
Division of Experimental Animal Research, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Ibaraki, Japan.
Kusubata
M
M
Yoshiki
A
A
Hiraiwa
N
N
Ohashi
T
T
Irie
S
S
Kusakabe
M
M
D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology* / Q000473:pathology; D004139:Dinitrofluorobenzene; D004423:Ear; D005838:Genotype; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D012867:Skin / Q000737:chemistry / Q000473:pathology; D019063:Tenascin / Q000172:deficiency* / Q000235:genetics; D013755:Tetradecanoylphorbol Acetate
D019063:Tenascin; D004139:Dinitrofluorobenzene; D013755:Tetradecanoylphorbol Acetate