title: Isolation and sequence analysis of the orotidine-5'-phosphate decarboxylase gene (URA3) of Candida utilis. Comparison with the OMP decarboxylase gene family.
abstract: The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is shown. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae.
authors.affiliation: Bioindustry Division, Center for Genetic Engineering and Biotechnology, Havana, Cuba. yeastlab@cigb.edu.cu
authors.forename: L
authors.initials: L
authors.lastname: Rodríguez
authors.forename: F P
authors.initials: FP
authors.lastname: Chávez
authors.forename: M E
authors.initials: ME
authors.lastname: González
authors.forename: L
authors.initials: L
authors.lastname: Basabe
authors.forename: T
authors.initials: T
authors.lastname: Rivero
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D002175:Candida / Q000235:genetics*; D004271:DNA, Fungal / Q000032:analysis; D005656:Fungal Proteins / Q000235:genetics*; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D014170:Transformation, Genetic
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D009964:Orotidine-5'-Phosphate Decarboxylase