title: Acute septicaemic pasteurellosis in Vietnamese pigs.
abstract: Sixteen isolates of Pasteurella multocida were cultured from cases diagnosed as acute septicaemic pasteurellosis in Vietnamese pigs. The HSB-PCR assay provided rapid presumptive determination of 10 isolates of P. multocida identified as haemorrhagic septicaemia (HS) causing type B cultures (B:2, B:5, B:2,5). Serological designation using the Carter and Heddleston typing systems confirmed these findings, and identified the six HSB-PCR negative cultures as either A:1, A:3 or D:3,4. Biochemical fermentation and REP-PCR revealed phenotypic and genotypic identity between P. multocida type A:1 isolated from Vietnamese pigs and poultry. Marked homogeneity was also demonstrated among HSB-PCR positive swine isolates, which were shown to possess genotypic identity with P. multocida type B:2 from buffaloes diagnosed with HS.
authors.affiliation: Division of Veterinary Pathology and Anatomy, University of Queensland, Brisbane, Australia. kirsty.townsend@mailbox.uq.edu.au
authors.forename: K M
authors.initials: KM
authors.lastname: Townsend
authors.forename: D
authors.initials: D
authors.lastname: O'Boyle
authors.forename: T T
authors.initials: TT
authors.lastname: Phan
authors.forename: T X
authors.initials: TX
authors.lastname: Hanh
authors.forename: T G
authors.initials: TG
authors.lastname: Wijewardana
authors.forename: I
authors.initials: I
authors.lastname: Wilkie
authors.forename: N T
authors.initials: NT
authors.lastname: Trung
authors.forename: A J
authors.initials: AJ
authors.lastname: Frost
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D002020:Buffaloes; D002645:Chickens; D017931:DNA Primers; D004372:Ducks; D010326:Pasteurella Infections / Q000175:diagnosis / Q000503:physiopathology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000503:physiopathology*; D014744:Vietnam
chemical_list: D017931:DNA Primers