[Detection of toxin producing strains of Clostridium difficile using rapid diagnostic methods].
Feces of 53 patients from different hospital wards suffering from long term post-antibiotic therapy diarrhea were tested. For direct detection of C. difficile toxin A, in samples TCD (Becton-Dickinson), and C. difficile Toxin A Test (Oxoid) tests were used. Toxin A was detected in 16 samples (29.6% tested). C. difficile strains were isolated from 40% of the fecal samples. Toxin A was detected in 25 Clostridium difficile strains with commercial tests and toxin B was detected using McCoy cell line. Toxin A was not produced by 3 C. difficile strains in vitro, but toxin B was produced by all strains. The polymerase chain reaction (PCR) test showed that all isolated strains possess genes of toxins A and B.
Pituch
H
H
Zakład Bakteriologii Klinicznej AM w Warszawie.
Obuch-Woszczatyński
P
P
Rouyan
G S
GS
Martirosian
G
G
Meisel-Mikołajczyk
F
F
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001427:Bacterial Toxins / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D016360:Clostridioides difficile / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D003428:Cross Infection / Q000382:microbiology*; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins
Wykrywanie toksynotwórczości szczepów Clostridium difficile przy pomocy szybkich metod diagnostycznych.