title: Specific detections of the early process of the glycation reaction by fructose and glucose in diabetic rat lens.
abstract: The glycation reaction by fructose, as well as that by glucose, in control and diabetic rat lens was analyzed by using antibodies which specifically recognize adducts of lysine with fructose and with glucose. Levels of fructose adducts in diabetic rat lens were 2.5 times that of the control, and correlated with sorbitol levels. This was mainly due to enhanced glycation of beta- and gamma-crystallins by fructose under diabetic conditions. These data suggest that glycation by fructose may also play a role in cataract formation under conditions of diabetes and aging.
authors.affiliation: Department of Biochemistry, Osaka University Medical School, Suita, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: J
authors.initials: J
authors.lastname: Fujii
authors.forename: N
authors.initials: N
authors.lastname: Miyazawa
authors.forename: A
authors.initials: A
authors.lastname: Hoshi
authors.forename: A
authors.initials: A
authors.lastname: Okado
authors.forename: Y
authors.initials: Y
authors.lastname: Tano
authors.forename: N
authors.initials: N
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D003459:Crystallins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006031:Glycosylation; D007908:Lens, Crystalline / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D010972:Platelet Activating Factor / Q000378:metabolism*; D051381:Rats; D012016:Reference Values; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D003459:Crystallins; D010972:Platelet Activating Factor; D005632:Fructose; D005947:Glucose; D008239:Lysine