Characterisation of a novel AGE-compound derived from lysine and 3-deoxyglucosone.
Dicarbonyl compounds are supposed to be reactive intermediates in the non-enzymatic glycation of proteins. A process that eventually could lead to the development of late diabetic complications. Glyoxal lysine dimer (GOLD) and methylglyoxal lysine dimer (MOLD) have previously been described as such reactive dicarbonyls. Here a new compound 3-deoxyglucosone lysine dimer (DOLD), a cross-link resulting from the reaction between hippuryl-lysine and 3-deoxyglucosone, has been isolated by HPLC and the structure determined by mass spectrometry and NMR.
Skovsted
I C
IC
Health Care Discovery, Novo Nordisk A/S, Bagsvaerd, Denmark.
Christensen
M
M
Breinholt
J
J
Mortensen
S B
SB
D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents; D003847:Deoxyglucose / Q000031:analogs & derivatives* / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000737:chemistry / Q000302:isolation & purification*; D008239:Lysine / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015416:Maillard Reaction; D015394:Molecular Structure; D016339:Spectrometry, Mass, Fast Atom Bombardment
D003432:Cross-Linking Reagents; D017127:Glycation End Products, Advanced; D003847:Deoxyglucose; C016350:3-deoxyglucosone; D008239:Lysine