title: The product of the mammalian orthologue of the Saccharomyces cerevisiae HBS1 gene is phylogenetically related to eukaryotic release factor 3 (eRF3) but does not carry eRF3-like activity.
abstract: We describe here the cloning and sequencing of human and mouse cDNAs encoding a putative GTP binding protein. Sequence comparison shows that these cDNAs (named eRFS) are likely to represent the orthologues of the yeast Saccharomyces cerevisiae HBS1 gene and that the C-terminal domains of the encoded proteins share structural features with eukaryotic elongation factor eEF-1A and release factor 3 (eRF3) families. The phylogenetic analysis suggests that eRFS proteins and Hbs1p form a cluster of orthologous sequences branching with the eRF3 family. Nevertheless, in yeast, the human eRFS protein and Hbs1p do not complement eRF3/Sup35p thermosensitive mutation and do not interact with eRF1.
authors.affiliation: Department of Internal Medicine I, University of Ulm, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Wallrapp
authors.forename: S B
authors.initials: SB
authors.lastname: Verrier
authors.forename: G
authors.initials: G
authors.lastname: Zhouravleva
authors.forename: H
authors.initials: H
authors.lastname: Philippe
authors.forename: M
authors.initials: M
authors.lastname: Philippe
authors.forename: T M
authors.initials: TM
authors.lastname: Gress
authors.forename: O
authors.initials: O
authors.lastname: Jean-Jean
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018388:Codon, Terminator; D018076:DNA, Complementary / Q000032:analysis; D005656:Fungal Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D019204:GTP-Binding Proteins; D015870:Gene Expression; D018840:HSP70 Heat-Shock Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010445:Peptide Elongation Factors; D010454:Peptide Termination Factors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000145:classification / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid
chemical_list: D018388:Codon, Terminator; D018076:DNA, Complementary; D005656:Fungal Proteins; C077247:HBS1 protein, S cerevisiae; D018840:HSP70 Heat-Shock Proteins; D010445:Peptide Elongation Factors; D010454:Peptide Termination Factors; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; C087971:peptide-chain-release factor 3; D019204:GTP-Binding Proteins