Effects of phosphorothioate oligodeoxyribonucleotide and oligoribonucleotides on human complement and coagulation.
We have synthesized and studied the effects of phosphorothioate (PS) oligodeoxyribonucleotide (DNA) and oligoribonucleotides (RNA, 2'-O-methyl-RNA and 2'-5'-RNA) on complement activation and prolongation of activated partial thromboplastin time (aPTT) in vitro. These results suggest that a PS-DNA prolongs aPTT, and inhibits complement lysis more than do the PS-RNA analogs.
Kandimalla
E R
ER
Hybridon, Inc., Cambridge, MA 02139, USA.
Shaw
D R
DR
Agrawal
S
S
D001483:Base Sequence; D001798:Blood Proteins / Q000378:metabolism*; D003167:Complement Activation / Q000187:drug effects*; D006801:Humans; D015394:Molecular Structure; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010314:Partial Thromboplastin Time; D011485:Protein Binding; D012709:Serum Albumin / Q000378:metabolism; D013329:Structure-Activity Relationship; D013873:Thionucleotides
D001798:Blood Proteins; D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D012709:Serum Albumin; D013873:Thionucleotides