[Spectrophotometric method for determination of sulfoxides].
Easy and exact method for determination of sulfoxides based on the property of this substances to react with the product of tryptophan's and p-dimethylaminobenzaldehyde's condensation is proposed. An oxidizer-deoxidizer reaction results in formation of bis-2-tryptophanyl-4-dimethylaminophenylcarbinol-blue-violet dye with the absorption maximum at 575 nm. Sensibility of method is 5 mkmol/g, relative mathematics error at three determinations is +/- 3.3%. Method can be used for studying of features of sulfoxides' biosynthesis and accumulation in garlic tissues and for estimation of quality of bulbs as row material for food and pharmaceutical industry.
Kosian
A M
AM
Taras Shevchenko Kyiv University.
D001547:Benzaldehydes / Q000737:chemistry; D007202:Indicators and Reagents; D010084:Oxidation-Reduction; D012680:Sensitivity and Specificity; D013053:Spectrophotometry; D013454:Sulfoxides / Q000032:analysis*; D014364:Tryptophan / Q000737:chemistry
D001547:Benzaldehydes; D007202:Indicators and Reagents; D013454:Sulfoxides; D014364:Tryptophan; C510241:p-dimethylaminobenzaldehyde
Spektrofotometrychnyĭ metod vyznachennia sul'foksydiv.