Carrier-mediated absorption of salicylic acid from hamster cheek pouch mucosa.
Previously, we found that monocarboxylic acids undergo carrier-mediated transport in primary cultures of oral mucosal epithelial cells.1 In this study, we investigated whether carrier-mediated absorption of a monocarboxylic acid from the oral mucosa occurs in vivo. Salicylic acid was administered to hamster cheek pouch. At predetermined intervals, the concentration of salicylic acid in the fluid remaining in the cheek pouch lumen and the blood salicylic acid concentration were determined. The absorption of salicylic acid was saturable at high salicylic acid concentrations. Sodium azide, a metabolic inhibitor, and carbonylcyanide p-trifluoromethoxyphenylhydrazone (FCCP), a protonophore, significantly inhibited the absorption of salicylic acid but not the absorption of salicylamide from the oral mucosa. Various monocarboxylic acids inhibited the absorption of salicylic acid, whereas dicarboxylic acids had no such effect. Transfer of [14C]salicylic acid from the cheek pouch mucosa to the systemic circulation was observed, and the blood [14C]salicylic acid concentration in the case of coadministration with propionic acid was significantly lower than that in the case of no propionic acid coadministration. These results show that monocarboxylic acids undergo carrier-mediated absorption from the hamster cheek pouch mucosa.
Utoguchi
N
N
Department of Pharmaceutics, Showa College of Pharmaceutical Sciences, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan.
Watanabe
Y
Y
Takase
Y
Y
Suzuki
T
T
Matsumoto
M
M
D000042:Absorption; D000818:Animals; D002264:Carboxylic Acids / Q000737:chemistry; D002478:Cells, Cultured; D002610:Cheek; D006224:Cricetinae; D004337:Drug Carriers; D004847:Epithelial Cells / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008647:Mesocricetus; D009061:Mouth Mucosa / Q000378:metabolism*; D020156:Salicylic Acid / Q000493:pharmacokinetics*; D013696:Temperature
D002264:Carboxylic Acids; D004337:Drug Carriers; D020156:Salicylic Acid