Biosynthesis of cobalamin in Salmonella typhimurium: transformation of riboflavin into the 5,6-dimethylbenzimidazole moiety.
In order to elucidate the biosynthesis of the base moiety of cobalamin in Salmonella typhimurium LT2, this organism was grown in the presence of [1'-14C]riboflavin. The vitamin B12 isolated was 14C-labeled. It was shown by chemical degradation that the 14C-label was exclusively localized in carbon atom 2 of the 5,6-dimethylbenzimidazole moiety. This demonstrated the precursor function of riboflavin in the biosynthesis of 5,6-dimethylbenzimidazole in S. typhimurium.
Keck
B
B
Institut für Biologische Chemie und Ernährungswissenschaft, Universität Hohenheim, Garbenstrasse 30, D-70599 Stuttgart, Germany.
Munder
M
M
Renz
P
P
D001562:Benzimidazoles / Q000378:metabolism*; D012256:Riboflavin / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000378:metabolism*; D014805:Vitamin B 12 / Q000096:biosynthesis*
D001562:Benzimidazoles; C015158:5,6-dimethylbenzimidazole; D014805:Vitamin B 12; D012256:Riboflavin