title: Peptidyl chemosensors incorporating a FRET mechanism for detection of Ni(II).
abstract: Hexapeptides incorporating two fluorophores flanking a tripeptide sequence that binds Ni(II) and Cu(II) with high affinity have been synthesized. While Cu(II) quenches the fluorescence of the resulting peptides, coordination of Ni(II) enables enhanced FRET (fluorescent resonance energy transfer) from one fluorophore to the other.
authors.affiliation: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena 91125, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Pearce
authors.forename: G K
authors.initials: GK
authors.lastname: Walkup
authors.forename: B
authors.initials: B
authors.lastname: Imperiali
mesh_terms: D000595:Amino Acid Sequence; D004735:Energy Transfer; D005456:Fluorescent Dyes; D009532:Nickel / Q000032:analysis*; D009842:Oligopeptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D005456:Fluorescent Dyes; D009842:Oligopeptides; D009532:Nickel