title: Synthesis of 14 C-radiolabeled oligonucleotides with a novel phosphoramidite reagent.
abstract: A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-14C]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus.
authors.affiliation: Department of Medicinal Chemistry, Isis Pharmaceuticals, Carlsbad, CA 92008.
authors.forename: A
authors.initials: A
authors.lastname: Guzaev
authors.forename: B
authors.initials: B
authors.lastname: Boyode
authors.forename: G
authors.initials: G
authors.lastname: Balow
authors.forename: K L
authors.initials: KL
authors.lastname: Tivel
authors.forename: M
authors.initials: M
authors.lastname: Manoharan
mesh_terms: D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds; D013936:Thymidine / Q000031:analogs & derivatives*
chemical_list: D002250:Carbon Radioisotopes; D007202:Indicators and Reagents; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; C028273:5'-O-dimethyltritylthymidine; D013936:Thymidine