Alz-50/Gallyas-positive lysosome-like intraneuronal granules in Alzheimer's disease and control brains.
The Gallyas-Braak silver impregnation method revealed neurons containing well-defined intraneuronal granules in both Alzheimer's disease and normal control brains. The granules were immunostained prominently with the Alz-50 antibody and, to a lesser degree, with the tau-2 antibody, but not with other anti-tau antibodies examined. The areas of distribution of granule-containing neurons detected by the Gallyas-Braak method appeared to overlap with the reported main sites of subcortical distribution of neurofibrillary tangles. They, however, were not observed in the cerebral cortex, including the hippocampal region. The Alz-50 immunoreactive granules showed ultrastructural features similar to those of lysosomes or lipofuscin. These findings suggest that denatured tau might be degraded in lysosomes.
Ikeda
K
K
Department of Neuropathology, Tokyo Institute of Psychiatry, Japan. kenikeda@prit.go.jp
Akiyama
H
H
Arai
T
T
Kondo
H
H
Haga
C
C
Iritani
S
S
Tsuchiya
K
K
D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000473:pathology*; D000906:Antibodies / Q000378:metabolism; D000941:Antigens / Q000378:metabolism*; D001921:Brain / Q000473:pathology* / Q000648:ultrastructure; D002402:Cathepsin D / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008247:Lysosomes / Q000648:ultrastructure*; D008526:Medulla Oblongata / Q000473:pathology / Q000648:ultrastructure; D008854:Microscopy, Electron; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D009847:Olivary Nucleus / Q000473:pathology; D011903:Raphe Nuclei / Q000473:pathology; D016622:Silver Staining; D016875:tau Proteins / Q000378:metabolism
C056167:Alzheimer's disease antigen; D000906:Antibodies; D000941:Antigens; D016875:tau Proteins; D002402:Cathepsin D