Infection and immunity mediated by the carbohydrate recognition domain of the Entamoeba histolytica Gal/GalNAc lectin.
Entamoeba histolytica causes invasive amebiasis, a major parasitic disease of the developing world, whose primary symptoms are liver abscess and colitis. All strains of E. histolytica express a 260-kDa surface Gal/GalNAc lectin that is antigenically conserved and immunogenic. The lectin is required for adherence to human intestinal epithelial cells and contact-dependent killing of immune effector cells. By expression cloning, the carbohydrate recognition domain (CRD) was identified within the lectin heavy-subunit cysteine-rich region. Of interest for a hepatic parasite, the CRD had sequence identity to the receptor-binding domain of hepatocyte growth factor (HGF) and competed with HGF for binding to the c-Met HGF receptor. In an animal model of invasive disease, immunization with the CRD inhibited liver-abscess formation, yet in humans, a naturally acquired immune response against the CRD did not persist.
Dodson
J M
JM
Department of Medicine, University of Virginia, Charlottesville, USA.
Lenkowski
P W
PW
Eubanks
A C
AC
Jackson
T F
TF
Napodano
J
J
Lyerly
D M
DM
Lockhart
L A
LA
Mann
B J
BJ
Petri
W A
WA
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001665:Binding Sites; D002118:Calcium / Q000378:metabolism; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000276:immunology*; D004748:Entamoeba histolytica / Q000276:immunology*; D004749:Entamoebiasis / Q000097:blood / Q000276:immunology*; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000008:administration & dosage / Q000276:immunology*; D008024:Ligands; D008562:Membrane Glycoproteins / Q000008:administration & dosage / Q000276:immunology*; D015800:Protozoan Proteins / Q000008:administration & dosage / Q000276:immunology*
D000953:Antigens, Protozoan; D002241:Carbohydrates; D037102:Lectins; D008024:Ligands; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; C052452:galactose inhibitable adherence protein, Entamoeba histolytica; D017228:Hepatocyte Growth Factor; D002118:Calcium