title: Focal transcriptional activity of murine cytomegalovirus during latency in the lungs.
abstract: Interstitial pneumonia is a frequent and critical manifestation of human cytomegalovirus (CMV) disease in immunocompromised patients, in particular in recipients of bone marrow transplantation. Previous work in the murine CMV infection model has identified the lungs as a major organ site of CMV latency and recurrence. It was open to question whether the viral genome is transcriptionally silent or active during latency. Transcription could be latency associated and thus be part of the latency phenotype. Alternatively, transcriptional activity could reflect episodes of reactivation. We demonstrate here that transcription of the immediate-early (IE) transcription unit ie1-ie3 selectively generates ie1-specific transcripts during latency. Notably, while the latent viral DNA was found to be evenly distributed in the lungs, transcription was focal and randomly distributed. This finding indicates that IE transcription is not a feature inherent to murine CMV latency but rather reflects foci of primordial reactivation. However, this reactivation did not initiate productive infection, since ie3 gene mRNA specifying the essential transactivator IE3 of murine CMV early gene expression was not detectable. Accordingly, transcripts encoding gB were absent during latency. By contrast, during induced virus recurrence, IE-phase transcription switched from focal to generalized and ie3-specific transcripts were generated. These data imply that latency and recurrence are regulated not only at the IE promoter-enhancer and that there exists an additional checkpoint at the level of precursor RNA splicing. We propose that focal transcription reflects random episodes of nonproductive reactivation that get terminated before IE3 is expressed and ignites the productive cycle.
authors.affiliation: Institute for Virology, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: S K
authors.initials: SK
authors.lastname: Kurz
authors.forename: M
authors.initials: M
authors.lastname: Rapp
authors.forename: H P
authors.initials: HP
authors.lastname: Steffens
authors.forename: N K
authors.initials: NK
authors.lastname: Grzimek
authors.forename: S
authors.initials: S
authors.lastname: Schmalz
authors.forename: M J
authors.initials: MJ
authors.lastname: Reddehase
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D005091:Exons; D005260:Female; D017781:Genes, Immediate-Early; D008168:Lung / Q000821:virology*; D008297:Male; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics* / Q000502:physiology; D012326:RNA Splicing; D012008:Recurrence; D014158:Transcription, Genetic; D017735:Virus Latency
chemical_list: D004279:DNA, Viral