[TGF-beta 1 gene expression in the healing process of skin wound in rat].
For observation of the change of transforming growth factor-beta 1 (TGF-beta 1) gene expression in the process of skin wound healing, the following experiments were performed. Sixteen Wistar rats were chosen. At each side of the rat's back, a 1 cm x 1.5 cm middle-thick skin wound was made. After 3, 6, 9 and 12 days, the specimens were taken from the wounds. For each specimen, half of it was used for RNA extraction, and underwent dot blotting; and the other half was frozen immediately and underwent in situ hybridization. The probes were dig-labeled PDGF-BB cDNA probe and TGF-beta 1 probe. The results showed that TGF-beta 1 gene was expressed mainly in fibroblast, epithelial cell and capillary endothelial cell. The peak of TGF-beta 1 mRNA content was in the 6th day postoperatively. After that, the content of TGF-beta 1 decreased to normal. It was suggested that TGF-beta 1 gene expression was in close relation with healing process. TGF-beta 1 may play an important regulatory role in the skin wound healing.
Wang
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Department of Plastic Surgery, Third Affiliated Hospital of Beijing Medical University, P.R. China.
Niu
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Cheng
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D000818:Animals; D015870:Gene Expression; D008297:Male; D012333:RNA, Messenger / Q000096:biosynthesis; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000293:injuries*; D016212:Transforming Growth Factor beta / Q000235:genetics*; D014945:Wound Healing; D014947:Wounds and Injuries / Q000378:metabolism
D012333:RNA, Messenger; D016212:Transforming Growth Factor beta