title: Macrolide biosynthesis: a single cytochrome P450, PicK, is responsible for the hydroxylations that generate methymycin, neomethymycin, and picromycin in Streptomyces venezuelae.
abstract: The final step in the biosynthesis of methymycin, neomethymycin, and picromycin is an hydroxylation, shown to be carried out by the cytochrome P-450 monooxygenase, PicK. Direct comparison of the relative Kcat/K(m) values for the two substrates, YC-17 and narbomycin, showed a threefold rate preference of picK for narbomycin.
authors.affiliation: Department of Chemistry, Brown University, Providence, RI 02912-9108, USA.
authors.forename: E I
authors.initials: EI
authors.lastname: Graziani
authors.forename: D E
authors.initials: DE
authors.lastname: Cane
authors.forename: M C
authors.initials: MC
authors.lastname: Betlach
authors.forename: J T
authors.initials: JT
authors.lastname: Kealey
authors.forename: R
authors.initials: R
authors.lastname: McDaniel
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001426:Bacterial Proteins; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000502:physiology*; D006900:Hydroxylation; D018942:Macrolides; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000502:physiology*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D018942:Macrolides; C008517:methymycin; C008594:neomethymycin; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; C115353:PicK protein, Streptomyces venezuelae; C003145:picromycin