Effects of low-frequency magnetic fields on fetal development in CBA/Ca mice.
Effects of alternating magnetic fields (MFs) on the embryonic and fetal development in CBA/Ca mice were studied. Mated females were exposed continuously to a sinusoidal 50 Hz (13 microT or 0.13 mT root mean square) or a sawtooth 20 kHz (15 microT peak-to-peak) MF from day 0 to day 18 of pregnancy for 24 h/day until necropsied on day 18. Control animals were kept under the same conditions without the MF. MFs did not cause maternal toxicity. No adverse effects were seen in maternal hematology and the frequency of micronuclei in maternal bone marrow erythrocytes did not change. The MFs did not increase the number of resorptions or fetuses with major or minor malformations in any exposure group. The mean number of implantations and living fetuses per litter were similar in all groups. The corrected weight gain (weight gain without uterine content) of dams, pregnancy rates, incidences of resorptions and late fetal deaths, and fetal body weights were similar in all groups. There was, however, a statistically significant increase in the incidence of fetuses with at least three skeletal variations in all groups exposed to MFs. In conclusion, the 50 Hz or 20 kHz MFs did not increase incidences of malformations or resorptions in CBA/Ca mice, but increased skeletal variations consistently in all exposure groups.
Huuskonen
H
H
National Public Health Institute, Division of Environmental Health, Laboratory of Toxicology, Kuopio, Finland.
Juutilainen
J
J
Julkunen
A
A
Mäki-Paakkanen
J
J
Komulainen
H
H
D000818:Animals; D001835:Body Weight; D001854:Bone Marrow Cells / Q000648:ultrastructure; D001842:Bone and Bones / Q000196:embryology; D002467:Cell Nucleus / Q000648:ultrastructure; D000013:Congenital Abnormalities / Q000209:etiology; D010064:Embryo Implantation; D005314:Embryonic and Fetal Development; D004912:Erythrocytes / Q000648:ultrastructure; D005260:Female; D005313:Fetal Death / Q000209:etiology; D005327:Fetal Resorption / Q000209:etiology; D005333:Fetus / Q000033:anatomy & histology; D008098:Litter Size; D008280:Magnetics; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains; D011247:Pregnancy