title: Aryl hydrocarbon hydroxylase activity in human placenta and threatened preterm delivery.
abstract: Induction of aryl hydrocarbon hydroxylase (AHH) activity in the placenta has been well documented. This enzyme may be induced by a variety of Polycyclic Aromatic Hydrocarbons (PAHs) and the AHH inducibility is associated with harmful effects of environmental chemicals. Toxic effects of PAHs in tissues such as placenta have been demonstrated to be due to their metabolites, epoxides, which interact with DNA. Thus, environmental PAHs may be related to its alterations in fetal development. Founded on these findings the PAH metabolites could interfere with the normal course of the pregnancy and may be an aborticide, a teratogen or a carcinogen. We hypothesize that low increased activity of placental Aryl Hydrocarbon Hydroxylase (AHH) may be an important determinant of human fetotoxicity. The present investigation was designed to examine the possible implications of PAH exposure at environmental exposure levels on the normal course of the pregnancy using AHH induction as an indicator of PAH exposure. Threatened Preterm Delivery (TPD) was used as an index of problems in the normal course of pregnancy. A group of forty pregnancies at term with TPD was compared with eighty controls for placental AHH induction. Macroscopic placental examination was also performed. A significant increase in prevalence of placental AHH induction with TPD was shown (Odds-Ratio = 2.8; 95% confidence bounds [1.3-6.2]; chi 2 = 6.7 p < 0.01). No such increases were found associated with placental pathology. When taking into account the group of placenta without basal plate calcifications, the significant increase in prevalence of placental AHH induction with TPD above mentioned was greatly increased (Odds-Ratio = 8.9; 95% confidence bounds [2.4-32.9]; chi 2 = 11.1 p < 0.001) controlling for gestational age. The increase in prevalence of placental AHH induction with TPD disappeared when taking into account the subgroup with basal plate or parenchyma calcifications. It is hypothesized that the high estrogen and progesterone at term may explain these associations.
authors.affiliation: Unité de Recherches en Epidémiologie, Institut National de la Santé et de la Recherche Médicale (INSERM), France.
authors.forename: G
authors.initials: G
authors.lastname: Huel
authors.forename: J
authors.initials: J
authors.lastname: Godin
authors.forename: N
authors.initials: N
authors.lastname: Frery
authors.forename: F
authors.initials: F
authors.lastname: Girard
authors.forename: T
authors.initials: T
authors.lastname: Moreau
authors.forename: C
authors.initials: C
authors.lastname: Nessmann
authors.forename: P
authors.initials: P
authors.lastname: Blot
mesh_terms: D000328:Adult; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis*; D002114:Calcinosis / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D018811:Maternal Exposure / Q000009:adverse effects*; D007752:Obstetric Labor, Premature / Q000139:chemically induced* / Q000201:enzymology*; D016017:Odds Ratio; D010920:Placenta / Q000737:chemistry* / Q000201:enzymology*; D010922:Placenta Diseases / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects*; D011247:Pregnancy; D015995:Prevalence
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D001189:Aryl Hydrocarbon Hydroxylases