title: Plasticity of the neoplastic phenotype in vivo is regulated by epigenetic factors.
abstract: Age of host and transplantation-site microenvironment influence the tumorigenic potential of neoplastically transformed liver epithelial cells. Tumorigenic BAG2-GN6TF rat liver epithelial cells consistently form tumors at ectopic sites, but differentially express tumorigenicity or hepatocytic differentiation in the liver depending on host age and route of cell transplantation into the liver. Direct inoculation into host livers concentrates tumor cells locally, resulting in undifferentiated tumors near the transplantation site in both young (3-month-old) and old (18-month-old) rats. Transplantation-site tumors regress within 1 month in the livers of young rats, but grow progressively in old rats. However, inoculation of cells into the spleen distributes transplanted cells individually throughout the liver, resulting in hepatocytic differentiation by tumor cells with concomitant suppression of their tumorigenicity in young rats. When transplanted into livers of old rats by splenic inoculation, or when young hepatic-transplant recipients are allowed to age, hepatocytic progeny of BAG2-GN6TF cells proliferate to form foci, suggesting that the liver microenvironment of old rats incompletely regulates the proliferation and differentiation of tumor cell-derived hepatocytes. Upon removal from the liver, BAG2-GN6TF-derived hepatocytes revert to an undifferentiated, aggressively tumorigenic phenotype. We posit that the spectrum between normal differentiation and malignant potential of these cells reflects the dynamic interaction of the specific transformation-related genotype of the cells and the characteristics of the tissue microenvironment at the transplantation site. Changes in the tissue milieu, such as those that accompany normal aging, may determine the ability of a genetically aberrant cell to produce a tumor.
authors.affiliation: Department of Pathology and Laboratory Medicine, Curriculum in Toxicology, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: McCullough
authors.forename: W B
authors.initials: WB
authors.lastname: Coleman
authors.forename: S L
authors.initials: SL
authors.lastname: Ricketts
authors.forename: J W
authors.initials: JW
authors.lastname: Wilson
authors.forename: G J
authors.initials: GJ
authors.lastname: Smith
authors.forename: J W
authors.initials: JW
authors.lastname: Grisham
mesh_terms: D000375:Aging; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000473:pathology*; D016031:Liver Transplantation / Q000473:pathology*; D008297:Male; D010641:Phenotype; D011183:Postoperative Complications / Q000473:pathology; D051381:Rats; D011916:Rats, Inbred F344