Separation of etoposide phosphate and methotrexate by capillary zone electrophoresis using UV detection with a high sensitivity cell.
Etoposide phosphate and methotrexate are important anti-tumor chemotherapeutic agents. Our previously presented capillary zone electrophoresis (CZE) method using a high sensitivity cell (Z-cell) for quantitative analysis in biological media (urine, plasma) showed good precision and accuracy. The present results show that the investigation using a capillary with high sensitivity cell led to an approximately 10-fold improvement of the detection limit compared to standard capillaries. Plasma and urine samples were analyzed by using a calibration curve for drug concentrations between 0.1 and 100 microg/mL. Good detection limits and good relative standard deviations of the migration times and of the peak areas were observed in these experiments.
Mrestani
Y
Y
Institute of Pharmaceutics and Biopharmaceutics, Martin-Luther-University, Halle/S., Germany.
Neubert
R
R
D000970:Antineoplastic Agents / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D019075:Electrophoresis, Capillary; D005047:Etoposide / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D005260:Female; D006801:Humans; D008727:Methotrexate / Q000097:blood / Q000302:isolation & purification* / Q000652:urine; D009943:Organophosphorus Compounds / Q000097:blood / Q000302:isolation & purification* / Q000652:urine
D000970:Antineoplastic Agents; D009943:Organophosphorus Compounds; C061400:etoposide phosphate; D005047:Etoposide; D008727:Methotrexate