Visualization of the Brassica self-incompatibility S-locus on identified oilseed rape chromosomes.
Seventy years after Karpechenko [15] first reported the accurate chromosome number of oilseed rape (Brassica napus L., 2n=38), we have developed a quantitative chromosome map of rape using computer imaging technology. The capacity to identify individual rape chromosomes will facilitate a wide range of genetic studies. Here we demonstrate the use of imaging methods in combination with fluorescence in situ hybridization to localize, on identified chromosomes, the single copy S-locus glycoprotein and S-locus-related genes involved in the self-incompatibility system of Brassica. These techniques have a broader application in plant genome research involving the mapping of single-copy genes and markers, irrespective of the plant species.
Kamisugi
Y
Y
Brassica & Oilseeds Research Department, John Innes Centre, Norwich, UK.
Nakayama
S
S
O'Neil
C M
CM
Mathias
R J
RJ
Trick
M
M
Fukui
K
K
D001937:Brassica / Q000235:genetics*; D002874:Chromosome Mapping; D004275:DNA, Ribosomal / Q000235:genetics; D006238:Haploidy; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D018517:Plant Roots; D018749:RNA, Plant / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics
D004275:DNA, Ribosomal; D018749:RNA, Plant; D012335:RNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D012341:RNA, Ribosomal, 5S; C065039:RNA, ribosomal, 25S