Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif.
We identified a novel gene encoding a RING finger (C3HC4-type zinc finger) protein from a human neuroblastoma full-length enriched cDNA library. This cDNA clone consists of 1919 nucleotides with an open reading frame of a 485-amino acid protein. From reverse transcription (RT)-polymerase chain reaction (PCR) analysis, the messenger RNA was ubiquitously expressed in various human adult tissues. The chromosomal location of the gene was determined on the chromosome 6p21.3 region by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel.
Seki
N
N
Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan.
Hattori
A
A
Sugano
S
S
Suzuki
Y
Y
Nakagawara
A
A
Ohhira
M
M
Muramatsu
M
M
Hori
T
T
Saito
T
T
D000328:Adult; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009447:Neuroblastoma / Q000235:genetics; D009687:Nuclear Proteins / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014407:Tumor Cells, Cultured; D016335:Zinc Fingers
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins