title: A simple and fast way to count heterophils in chemotaxis.
abstract: Heterophils were labeled with fluorescein isothiocyanate and then placed in a modified Boyden chamber to determine their response to chemoattractants. After incubation, the cells that had migrated into the membrane were examined under an epifluorescent microscope, and the image was captured by a charge-coupled digital camera and a frame grabber and saved. The saved images were then counted by NIH Image (a public domain software). These modifications greatly speed up the counting of cells and relieve the eyestrain suffered among workers in the chemotaxis field.
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis 95616, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Lam
mesh_terms: D000818:Animals; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002645:Chickens; D016650:Fluorescein-5-isothiocyanate; D016209:Interleukin-8 / Q000494:pharmacology; D007958:Leukocyte Count / Q000379:methods*; D007962:Leukocytes / Q000187:drug effects / Q000502:physiology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D013047:Specific Pathogen-Free Organisms
chemical_list: D016209:Interleukin-8; D008070:Lipopolysaccharides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D016650:Fluorescein-5-isothiocyanate