Analysis of Legionella pneumophila serogroup 1 isolates by pulsed-field gel electrophoresis.
At a hospital in Halifax, Nova Scotia, Canada, three strains of Legionella pneumophila were detectable based on plasmid content, while the isolates collected at another hospital in Halifax had no plasmids. Genomic DNA was digested with BssHII, SalI, and SpeI and subjected to pulsed-field gel electrophoresis (PFGE). We found no relationship between plasmid profile and PFGE pattern.
Marrie
T J
TJ
Departments of Medicine, Dalhousie University and Queen Elizabeth II Health Sciences Centre, Ottawa, Ontario, Canada. tmarrie@is.dal.ca
Tyler
S
S
Bezanson
G
G
Dendy
C
C
Johnson
W
W
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping
D004269:DNA, Bacterial