title: New York 1 and Sin Nombre viruses are serotypically distinct viruses associated with hantavirus pulmonary syndrome.
abstract: New York 1 virus (NY-1) and Sin Nombre virus (SN) are associated with hantavirus pulmonary syndrome (HPS). NY-1 and SN are derived from unique mammalian hosts and geographic locations but have similar G1 and G2 surface proteins (93 and 97% identical, respectively). Focus reduction neutralization assays were used to define the serotypic relationship between NY-1 and SN. Sera from NY-1-positive Peromyscus leucopus neutralized NY-1 and SN at titers of >/=1/3,200 and </=1/400, respectively (n = 12). Conversely, SN-specific rodent sera neutralized NY-1 and SN at titers of <1/400 and 1/6,400, respectively (n = 13). Acute-phase serum from a New York HPS patient neutralized NY-1 (1/640) but not SN (<1/20), while sera from HPS patients from the southwestern United States had 4- to >16-fold-lower neutralizing titers to NY-1 than to SN. Reference sera to Hantaan, Seoul, and Prospect Hill viruses also failed to neutralize NY-1. These results indicate that SN and NY-1 define unique hantavirus serotypes and implicate the presence of additional HPS-associated hantavirus serotypes in the Americas.
authors.affiliation: The Department of Medicine, Stony Brook University, Stony Brook, New York 11794, USA.
authors.forename: I
authors.initials: I
authors.lastname: Gavrilovskaya
authors.forename: R
authors.initials: R
authors.lastname: LaMonica
authors.forename: M E
authors.initials: ME
authors.lastname: Fay
authors.forename: B
authors.initials: B
authors.lastname: Hjelle
authors.forename: C
authors.initials: C
authors.lastname: Schmaljohn
authors.forename: R
authors.initials: R
authors.lastname: Shaw
authors.forename: E R
authors.initials: ER
authors.lastname: Mackow
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000097:blood; D002522:Chlorocebus aethiops; D006476:Orthohantavirus / Q000145:classification* / Q000276:immunology; D018804:Hantavirus Pulmonary Syndrome / Q000097:blood / Q000276:immunology / Q000473:pathology / Q000821:virology*; D006801:Humans; D019590:Nucleocapsid Proteins / Q000032:analysis / Q000276:immunology; D010542:Peromyscus; D012703:Serotyping; D014709:Vero Cells
chemical_list: D000956:Antigens, Viral; D019590:Nucleocapsid Proteins