High expression of glycosphingolipids involved in procoagulant activity of cancer cells.
To investigate the involvement of tumor-associated antigens such as sLe(a) and sLe(x), as well as Le(y) glycosphingolipid in the procoagulant activity of cancer cells, we examined the relationship between the inhibitory effect of anti-sLe(a) or anti-sLe(x) antibodies on the procoagulant activity of cancer cells, and cell surface expression of sLe(a) and sLe(x) antigens. We observed that the procoagulant activity of cancer cells which highly expressed sLe(a) or sLe(x) antigens (expression rate >70%) was significantly inhibited by the relevant antibodies. These data suggested that not only Le(y) but also sLe(a) and sLe(x) antigens play an important role in coagulation induced by various cancer cells.
Suzuki
M
M
Fujisaki Cell Center, Hayashibara Biochemical Laboratories Inc., Okayama 702-8006, Japan.
Ando
O
O
Ohta
T
T
Inufusa
H
H
Adachi
T
T
Yasutomi
M
M
Kurimoto
M
M
D000230:Adenocarcinoma / Q000737:chemistry / Q000473:pathology; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000494:pharmacology; D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D001780:Blood Coagulation Tests; D018395:CA-19-9 Antigen; D006528:Carcinoma, Hepatocellular / Q000737:chemistry / Q000473:pathology; D003110:Colonic Neoplasms / Q000737:chemistry / Q000473:pathology; D003546:Cysteine Endopeptidases / Q000032:analysis / Q000502:physiology*; D005732:Gangliosides / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006017:Glycolipids / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D008113:Liver Neoplasms / Q000737:chemistry / Q000473:pathology; D008175:Lung Neoplasms / Q000737:chemistry / Q000473:pathology; D009363:Neoplasm Proteins; D000080506:Sialyl Lewis X Antigen; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D018395:CA-19-9 Antigen; D005732:Gangliosides; D006017:Glycolipids; D007983:Lewis Blood Group Antigens; C094162:Lewis Y antigen; D009363:Neoplasm Proteins; D000080506:Sialyl Lewis X Antigen; C094688:sialyl Lewis(x) ganglioside; C064564:sialyl Le(a) ganglioside; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant