Prechallenge high neutralizing antibodies and long-lasting immune reactivity to gp41 correlate with protection of rhesus monkeys against productive simian immunodeficiency virus infection or disease development.
To investigate the protective efficacy of various gp130 vaccine preparations, rhesus monkeys were immunized with gp130 oligomers (O-gp130) or two different gp130-monomer preparations (M1-gp130; M2-gp130) and challenged with 50 MID50 of simian immunodeficiency virus (SIV)mac32H. Following challenge the control animals and all animals of the M1- and M2-gp130 group and 1 animal of the O-gp130 group were productively infected, whereas 3 animals of the O-gp130 group resisted the productive virus replication. The protection was correlated with high neutralizing antibodies and a long-lasting immune response to the transmembrane protein gp41. Whereas none of the O-gp130 animals had developed disease symptoms, 3 M1-gp130 animals, 1 M2-gp130 animal, and 2 control animals died as a result of AIDS within 18 months after challenge. Therefore, immunization with virion-derived gp130 oligomers of SIVmac32H can confer protection against the productive infection with SIVmac32H and suppress the development of the AIDS-like disease.
Petry
H
H
Department of Virology and Immunology, German Primate Center, Göttingen.
Dittmer
U
U
Jones
D
D
Farrar
G
G
Wachter
H
H
Fuchs
D
D
Nisslein
T
T
Jurkiewicz
E
E
Hunsmann
G
G
Stahl-Hennig
C
C
Lüke
W
W
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000918:Antibody Specificity; D015153:Blotting, Western; D004279:DNA, Viral / Q000097:blood; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D015686:Gene Products, env / Q000276:immunology; D007106:Immune Sera / Q000276:immunology; D008253:Macaca mulatta; D008562:Membrane Glycoproteins / Q000276:immunology; D019798:Neopterin / Q000652:urine; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D015531:Radioimmunoprecipitation Assay; D012191:Retroviridae Proteins / Q000276:immunology; D018449:SAIDS Vaccines; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D019562:Viral Load; D014779:Virus Replication
D000914:Antibodies, Viral; D004279:DNA, Viral; D015686:Gene Products, env; D007106:Immune Sera; D008562:Membrane Glycoproteins; D012191:Retroviridae Proteins; D018449:SAIDS Vaccines; C082614:SIV envelope protein gp41; C078684:simian immunodeficiency virus gp130; D019798:Neopterin