Reversal of hyperglycemia in streptozotocin diabetic mice by xenotransplantation of microencapsulated rat islets.
Rat pancreatic islets were immunoisolated within alginate capsules with additional polyethyleneimine-protamine-heparin highly biocompatible membrane. Perifusion study in vitro demonstrated satisfactory similarities between the insulin release profiles of encapsulated and free islets. Concordant xenotransplantation of microencapsulated rat islets significantly prolonged mean time of restored normoglycemia (46 +/- 15 days) in streptozotocin-diabetic BALB/c mice recipients comparing to uncoated grafts (7 +/- 2 days).
Tatarkiewicz
K
K
Institute of Biocybernetics & Biomedical Engineering, Warsaw, Poland.
Sitarek
E
E
Sabat
M
M
Orłowski
T
T
D000464:Alginates; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000601:surgery*; D020723:Glucuronic Acid; D006493:Heparin; D006603:Hexuronic Acids; D006943:Hyperglycemia / Q000601:surgery*; D016381:Islets of Langerhans Transplantation; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008863:Microspheres; D019397:Pancreas, Artificial; D011094:Polyethyleneimine; D011108:Polymers; D011479:Protamines; D051381:Rats; D014183:Transplantation, Heterologous
D000464:Alginates; D001786:Blood Glucose; D006603:Hexuronic Acids; D011108:Polymers; D011479:Protamines; D020723:Glucuronic Acid; D011094:Polyethyleneimine; D006493:Heparin