title: Progesterone 6-hydroxylation is catalysed by cytochrome P-450 in the moderate thermophile Bacillus thermoglucosidasius strain 12060.
abstract: The moderate thermophile, Bacillus thermoglucosidasius, transforms progesterone into four metabolites. These are 6alpha- and 6beta-hydroxyprogesterone, androstenedione and testosterone. This is the first report of bacterial 6alpha-hydroxylation of steroids. The identity of the progesterone metabolites shows that there are three major types of transforming activity in this organism; C-17-C-20 lyase that cleaves the pregnane side chain of the substrate, C-17 oxidoreductase that interconverts the metabolites androstenedione and testosterone, and 6-hydroxylation. 6-hydroxylation activity was purified virtually to homogeneity and was shown to be catalysed by a cytochrome P-450 monooxygenase enzyme. This is the first report of a thermostable cytochrome P-450.
authors.affiliation: Department of Biochemistry, Queen Mary and Westfield College, London, UK.
authors.forename: O
authors.initials: O
authors.lastname: Sideso
authors.forename: R A
authors.initials: RA
authors.lastname: Williams
authors.forename: S G
authors.initials: SG
authors.lastname: Welch
authors.forename: K E
authors.initials: KE
authors.lastname: Smith
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D006900:Hydroxylation; D011374:Progesterone / Q000378:metabolism*
chemical_list: D011374:Progesterone; D003577:Cytochrome P-450 Enzyme System