Estimation of bacterial contamination in ultrapure water: application of the anti-DNA antibody.
We constructed and established a hybridoma cell line that produces immunoglobulin G-type anti-DNA antibody. By using this antibody, we could successfully detect a single bacterial cell in ultrapure water (UPW). The detection system is composed of a membrane-supported western blotting-type immunoassay and a two-dimensional photon analyzer with high resolution. It can detect and count every bacterial cell in a wide field of view on a trapping filter i.e., a circle with an 18-mm diameter. This means 10 fg (10(-14) g) of bacterial DNA can be detected in the field. This system could be a useful tool for evaluating the number of bacteria contained by UPW and water used for medical purposes.
Nogami
T
T
Central Research Laboratories, Organo Corporation, Saitama, Japan.
Ohto
T
T
Kawaguchi
O
O
Zaitsu
Y
Y
Sasaki
S
S
D000818:Animals; D000974:Antibodies, Antinuclear / Q000276:immunology*; D004269:DNA, Bacterial / Q000032:analysis*; D004926:Escherichia coli / Q000235:genetics; D006735:Horseradish Peroxidase; D051379:Mice; D008807:Mice, Inbred BALB C; D014407:Tumor Cells, Cultured; D014871:Water Microbiology
D000974:Antibodies, Antinuclear; D004269:DNA, Bacterial; D006735:Horseradish Peroxidase