title: Human adrenocortical NCI-H295 cells express VIP receptors. Steroidogenic effect of vasoactive intestinal peptide (VIP).
abstract: VIP receptors are frequently overexpressed by various endocrine tumors. In this study the expression of VIP receptors in the human adrenocortical carcinoma cell line NCI-H295 and their involvement in the regulation of steroidogenesis was investigated. NCI-H295 cells express VIP1 and VIP2 receptors as demonstrated by RT-PCR, whereas they do not express VIP itself. The receptors are functionally coupled to steroidogenesis since VIP (10(-9) M to 10(-6) M) exerted a dose-dependent stimulatory effect on the release of aldosterone, cortisol, and DHEA. VIP increased ACTH-stimulated releases of aldosterone and cortisol. The proliferation rate of NCI-H295 cells was not affected by VIP. These data show that NCI-H295 cells express both forms of the VIP receptor and that VIP is involved in an ACTH-independent regulation of steroidogenesis in the adrenal tumor cells.
authors.affiliation: Department of Internal Medicine III, University of Leipzig, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Haidan
authors.forename: U
authors.initials: U
authors.lastname: Hilbers
authors.forename: S R
authors.initials: SR
authors.lastname: Bornstein
authors.forename: M
authors.initials: M
authors.lastname: Ehrhart-Bornstein
mesh_terms: D000302:Adrenal Cortex / Q000187:drug effects*; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis*; D000306:Adrenal Cortex Neoplasms / Q000378:metabolism; D000450:Aldosterone / Q000096:biosynthesis; D003687:Dehydroepiandrosterone / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006854:Hydrocortisone / Q000096:biosynthesis; D018005:Receptors, Vasoactive Intestinal Peptide / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects* / Q000473:pathology; D014660:Vasoactive Intestinal Peptide / Q000494:pharmacology*
chemical_list: D000305:Adrenal Cortex Hormones; D018005:Receptors, Vasoactive Intestinal Peptide; D014660:Vasoactive Intestinal Peptide; D003687:Dehydroepiandrosterone; D000450:Aldosterone; D006854:Hydrocortisone