Effect of inhibition of glucose and fat metabolism on galanin-R1 receptor mRNA levels in the rat hypothalamic paraventricular and supraoptic nuclei.
Using in situ hybridization histochemistry we have studied the effect of glucose and fat deprivation on galanin-R1 receptor (GAL-R1-R) mRNA levels in the rat paraventricular (PVN) and supraoptic (SON) nuclei after single and repeated i.p. administration of a glucose antimetabolite 2-deoxy-D-glucose (DG), as well as of a fatty acid antimetabolite, sodium mercaptoacetate (MA), treatments known to increase food intake. Both DG and MA injections caused an increase in levels of GAL-R1 mRNA transcripts in the PVN and SON. These results indicate that glucose and fat deprivation increase the sensitivity of PVN and SON neurons to galanin, and that regulation of receptor levels may be an important mechanism in galaninergic signalling.
Gorbatyuk
O
O
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Hökfelt
T
T
D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects / Q000502:physiology; D003847:Deoxyglucose / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology; D005223:Fats / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D005947:Glucose / Q000378:metabolism*; D017403:In Situ Hybridization; D008297:Male; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide / Q000235:genetics*; D013495:Supraoptic Nucleus / Q000737:chemistry / Q000378:metabolism*; D013864:Thioglycolates / Q000494:pharmacology
D005223:Fats; D012333:RNA, Messenger; D044088:Receptors, Galanin; D018013:Receptors, Neuropeptide; D013864:Thioglycolates; C017487:2-mercaptoacetate; D003847:Deoxyglucose; D019204:GTP-Binding Proteins; D005947:Glucose