title: Effectiveness of four chemical solutions in eliminating Bacillus subtilis spores on gutta-percha cones.
abstract: Gutta-percha cones should be free of pathogenic micro-organisms before being used for root canal filling. This study was carried out to evaluate the effectiveness of four chemical agents in eliminating Bacillus subtilis spores from gutta-percha cones. The solutions tested were 5.25% sodium hypochlorite, 2% glutaraldehyde, 2% chlorhexidine digluconate, and 70% ethyl alcohol. The gutta-percha cones coated with spores were placed into contact with the chemical agents for 1, 3, 5 and 10 min. The results showed that 5.25% sodium hypochlorite was effective in destroying the spores after 1 min of contact. Glutaraldehyde, chlorhexidine and ethyl alcohol did not decontaminate the gutta-percha cones even after 10 min of contact.
authors.affiliation: Institute of Microbiology Paulo de Góes, Federal University of Rio de Janeiro, Brazil.
authors.forename: J F
authors.initials: JF
authors.lastname: Siqueira
authors.forename: C H
authors.initials: CH
authors.lastname: da Silva
authors.forename: D
authors.initials: D
authors.lastname: Cerqueira M das
authors.forename: H P
authors.initials: HP
authors.lastname: Lopes
authors.forename: M
authors.initials: M
authors.lastname: de Uzeda
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology; D019551:Dental Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006171:Gutta-Percha; D012973:Sodium Hypochlorite / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects
chemical_list: D019551:Dental Disinfectants; D000431:Ethanol; D006171:Gutta-Percha; D012973:Sodium Hypochlorite; D002710:Chlorhexidine; D005976:Glutaral