Correlation between expression of mdr-1 gene and oncogenes in human promyelocytic leukemic HL60 cell line and sublines.
OBJECTIVE
To study the relationship between expression of oncogenes and multiple drug resistant (MDR) phenotype.


METHODS
The drug resistant level of HL60 cell line and its sublines were determined with flow cytometry. RNA Dot blot hybridization was used to identify the expression of oncogenes and mdr-1 gene.


RESULTS
The expression of mdr-1 gene was in the opposite relation with c-myc expression, but in the positive relation with c-H-ras gene expression in the multiple drug resistant cell lines. In non-MDR cell line HL60/RA, the expression levels of mdr-1, c-myc, and c-H-ras were the same as HL60 parental cells.


CONCLUSIONS
Multiple drug resistance is related to not only mdr-1 expression, but also some oncogenes expression level.
Zhou
W D
WD
Department of Biology, Beijing Normal University, China.
Zhang
H Q
HQ
Fang
M
M
Xue
S B
SB
D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D015870:Gene Expression; D019450:Genes, MDR; D016259:Genes, myc; D011905:Genes, ras; D018922:HL-60 Cells; D006248:Harringtonines / Q000494:pharmacology; D006801:Humans; D016271:Proto-Oncogene Proteins c-myc / Q000096:biosynthesis / Q000235:genetics; D016283:Proto-Oncogene Proteins p21(ras) / Q000096:biosynthesis / Q000235:genetics; D014700:Verapamil / Q000494:pharmacology
D006248:Harringtonines; D016271:Proto-Oncogene Proteins c-myc; D014700:Verapamil; C501009:HRAS protein, human; D016283:Proto-Oncogene Proteins p21(ras)