Thermostable DNA-polymerase from Thermus thermophilus B35: isolation and characterization of some properties.
Thermostable Tte DNA-polymerase was isolated from the strain Thermus thermophilus B35 which was found in hot spring water. The enzyme with molecular mass 87 kD was isolated using sequential chromatography on DEAE-Sepharose, hydroxylapatite, hexyl-agarose, and heparin-Sepharose. Biochemical properties of Tte DNA-polymerase are similar to those of Tth DNA-polymerase isolated from Thermus thermophilus HB8; however, practical application of Tte-Pol seems to be more favorable due to higher temperature optimum of this enzyme and lack of restriction endonucleases in the initial strain.
Rechkunova
N I
NI
Novosibirsk Institute of Bioorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Akishev
A G
AG
Lebedeva
N A
NA
Khodyreva
S N
SN
Degtyarev
S Kh
SKh
Lavrik
O I
OI
D002413:Cations, Divalent / Q000494:pharmacology; D002845:Chromatography; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D017886:Durapatite; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D008970:Molecular Weight; D013816:Thermodynamics; D016963:Thermus thermophilus / Q000201:enzymology*
D002413:Cations, Divalent; D017886:Durapatite; D004259:DNA-Directed DNA Polymerase