title: Identification of the three non-identical subunits constituting human deoxyribonuclease II.
abstract: We purified DNase II from human liver to apparent homogeneity. The N-terminal amino acid sequences of each of three components constituting the purified mature enzyme were then separately determined by automatic Edman degradation. A combination of this chemical information and the previously reported nucleotide sequence of the cDNA encoding human DNase II [Yasuda et al. (1998) J. Biol. Chem. 273, 2610-2626] allowed detailed elucidation of the enzyme's subunit structure: human DNase II was composed of three non-identical subunits, a propeptide, proprotein and mature protein, following a signal peptide. Expression analysis of a series of deletion mutants derived from the cDNA of DNase II in COS-7 cells suggested that although a single large precursor protein may not be necessary for proteolytic maturation, the propeptide region L17-Q46 may play an essential role in generating the active form of the enzyme.
authors.affiliation: Department of Legal Medicine, Gunma University School of Medicine, Maebashi, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takeshita
authors.forename: T
authors.initials: T
authors.lastname: Yasuda
authors.forename: R
authors.initials: R
authors.lastname: Iida
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: O
authors.initials: O
authors.lastname: Hosomi
authors.forename: Y
authors.initials: Y
authors.lastname: Nakashima
authors.forename: S
authors.initials: S
authors.lastname: Mori
authors.forename: H
authors.initials: H
authors.lastname: Nomoto
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D004706:Endodeoxyribonucleases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004792:Enzyme Precursors / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D006801:Humans; D008099:Liver / Q000201:enzymology; D008875:Middle Aged; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D021382:Protein Sorting Signals; D017421:Sequence Analysis; D017384:Sequence Deletion; D014162:Transfection
chemical_list: D004792:Enzyme Precursors; D021382:Protein Sorting Signals; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II