title: Identification and characterization of the tumor suppressor p53 in channel catfish (Ictalurus punctatus).
abstract: Herein is presented the sequence of a catfish full-length p53 cDNA obtained from a cloned B cell line cDNA library. Southern blot analyses determined that a restriction fragment linked polymorphism (RFLP) existed with PstI among outbred catfish. Western blot analyses demonstrated that, when compared to PBLs, the catfish leukocyte lines express higher levels of p53 protein. Additionally, the results of Western blot analyses and in vitro translation experiments suggest that the catfish leukocyte lines may produce truncated forms of p53 due to internal initiation.
authors.affiliation: Department of Microbiology, University of Mississippi Medical Center, Jackson 39216, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: Luft
authors.forename: E
authors.initials: E
authors.lastname: Bengtén
authors.forename: L W
authors.initials: LW
authors.lastname: Clem
authors.forename: N W
authors.initials: NW
authors.lastname: Miller
authors.forename: M R
authors.initials: MR
authors.lastname: Wilson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary / Q000235:genetics; D016158:Genes, p53; D006801:Humans; D007059:Ictaluridae / Q000235:genetics* / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008969:Molecular Sequence Data; D012150:Polymorphism, Restriction Fragment Length; D014176:Protein Biosynthesis; D017386:Sequence Homology, Amino Acid; D013045:Species Specificity; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D016159:Tumor Suppressor Protein p53