Detection of active oxygen species in biological systems.
1. Cypridina luciferin analogues, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLD) and 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one(CLA ), react with O2- or 1O2 to emit light in visible region. Such chemiluminescences were used for the detection of O2- or 1O2 in activated leukocyte systems and myeloperoxidase (granulocyte-extract) + Br- + H2O2 systems in vitro. 2. The mechanisms of MCLA (CLA)-dependent luminescence is described in detail. Superoxide generated from sinusoidal cells in acute ethanol intoxication of rats was detected by MCLA-dependent luminescence from the surface of perfused rat liver (organ luminescence). 3. Furthermore, with alive animals, O2- generated in the lung of rats with necrotized pancreatitis and that in the stomach of rats after ischemia/reperfusion were detected by their organ luminescences.
Nakano
M
M
Department of Photon and Free Radical Research, Japan Immunoresearch Laboratories Co., Ltd., Gunma, Japan.
D000435:Alcoholic Intoxication / Q000378:metabolism; D000818:Animals; D049454:Firefly Luciferin / Q000031:analogs & derivatives; D005753:Gastric Mucosa / Q000378:metabolism; D006098:Granulocytes / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000502:physiology*; D008099:Liver / Q000502:physiology; D008163:Luminescent Measurements; D008168:Lung / Q000502:physiology; D009336:Necrosis; D009504:Neutrophils / Q000502:physiology; D010195:Pancreatitis / Q000175:diagnosis / Q000473:pathology; D009195:Peroxidase / Q000097:blood; D051381:Rats; D017382:Reactive Oxygen Species; D015427:Reperfusion Injury / Q000175:diagnosis / Q000378:metabolism; D013270:Stomach / Q000098:blood supply; D013481:Superoxides / Q000032:analysis* / Q000097:blood
D017382:Reactive Oxygen Species; D013481:Superoxides; D049454:Firefly Luciferin; D009195:Peroxidase