Effect of norepinephrine on ouabain-sensitive, K+-dependent p-nitrophenylphosphatase activity in strial marginal cells of the cochlea in normal and reserpinized guinea pigs.
On the basolateral infoldings of the strial marginal cells in the cochlea, Na K ATPase activity is abundant. To clarify the humoral control by norepinephrine, K-NPPase activity of strial marginal cells in the cochlea was investigated in normal, reserpine, norepinephrine (NE), reserpine plus NE-treated guinea pigs using a cerium-based method. K-NPPase activity was almost completely decreased 3-20 days after reserpine administration. At 10 days after reserpinization and following NE repeated treatment, enzyme activity was detectable. These results suggested that norepinephrine might restore and regulate strial K-NPPase activity.
Kanoh
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Department of Otolaryngology, Hyogo College of Medicine, Nishinomiya, Japan.
D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors / Q000187:drug effects*; D018759:Adrenergic Uptake Inhibitors / Q000494:pharmacology*; D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D002563:Cerium; D003593:Cytoplasm / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D004793:Enzyme Reactivators / Q000494:pharmacology; D006168:Guinea Pigs; D006651:Histocytochemistry; D008854:Microscopy, Electron; D009638:Norepinephrine / Q000494:pharmacology*; D010042:Ouabain / Q000494:pharmacology*; D012110:Reserpine / Q000494:pharmacology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects; D013316:Stria Vascularis / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure
D018759:Adrenergic Uptake Inhibitors; D000316:Adrenergic alpha-Agonists; D004791:Enzyme Inhibitors; D004793:Enzyme Reactivators; D002563:Cerium; D010042:Ouabain; D012110:Reserpine; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D009638:Norepinephrine