[Effect of tocopherol deficiency and additional administration of tocopherol, dibunol and disodium selenite on enzyme activity in rat liver, which take part in the biotransformation of nitrosodimethylamine].
The alimentary tocopherol deficiency is accompanied by decreased hydroxylase, demethylase, NADH- and NADPH-reductase, aldehyde dehydrogenase, arylesterase and glutathione reductase activity in rat's liver. It decreased the reduced glutathione and increased it's oxidized form concentration in the tocopherol deficient animals. The stability of microsomal membrane is decreased to solubilizing action of deoxycholate and trypsin. This changes, possibly, caused elevation of alteration of function enzyme's and microsomal membrane after nitrosodimethylamine (NDMA) administration in deficient rats. The 7-days injection of tocopherol (20 and 100 mg/kg), dibunol (80 mg/kg), sodium selenite (30 mkg/kg) increased aldehyde dehydrogenase, esterase, glutathione-dependent enzymes activity and increased of reduced glutathione concentration in liver, suppressed lipid peroxidation and increased survival rats after lethal dose carcinogen treatment. Supplementation of tocopherol decreased harmful action of nitrosodimethylamine on microsomal membrane and enzymes activity.
Isotshyn
V M
VM
M.I. Pirogova Medical University, Vinniza.
Pentiuk
O O
OO
Matviĭchuk
M V
MV
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001711:Biotransformation; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D002273:Carcinogens / Q000493:pharmacokinetics; D004128:Dimethylnitrosamine / Q000493:pharmacokinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D018038:Sodium Selenite / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology*; D014811:Vitamin E Deficiency / Q000188:drug therapy*
D000975:Antioxidants; D002273:Carcinogens; D014810:Vitamin E; D002084:Butylated Hydroxytoluene; D018038:Sodium Selenite; D004128:Dimethylnitrosamine
Vplyv defitsytu tokoferolu ta dodatkovoho vvedennia tokoferolu, dibunolu, selenitu natriiu na aktyvnist' fermentiv pechinky shchuriv, shcho berut' uchast' u biotransformatsiï nitrozodymetylaminu.