[Effects of inhalated isosorbide dinitrate on endothelin and nitric oxide and its therapeutic efficacy for experimental guinea-pigs with asthma].
To probe into the correlation between the changes of endothelin and nitric oxide in bronchial alveolar lavage fluid (BALF) and the treatment of inhaling isosorbide dinitrate on experimental guinea pigs with asthma, we divided 25 guinea pigs randomly into three groups (Control group, Asthma group, Isosorbide dinitrate group), and induced some subjects by inhalation of aerosolized ovalbumin as the model of asthma to investigate the endothelin (ET) and nitric oxide (NO) level in BALF as well as the pathological change of the respiratory tract. We found that the level of endothelin and nitric oxide in BALF were all elevated in experimental guinea pigs with asthma, but the degree of increase of NO in BALF was lower than that of ET. The NO and the ET levels in BALF were negative correlation (r = -0.8050, P < 0.01). After having been treated with aerosolized isosorbide dinitrate, the NO level in BALF was significantly elevated and the ET level in BALF was decreased in comparison with the asthma group (P < 0.001, respectively). Moreover, the eosinophile infiltration, exoserosis, and epithalaxia of the respiratory tract of the guinea-pigs were all lightened. The results suggest that inhaled isosoratory dinitrate could be used for treating asthma, and its mechanism may be elevation of NO level and decrease of ET level in BALF.
Xiang
X
X
Department of Internal Medicine, Second Affiliated Hospital, Hunan Medical University, Changsha.
Zhou
H
H
Yao
W
W
D000280:Administration, Inhalation; D000818:Animals; D001249:Asthma / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D001993:Bronchodilator Agents / Q000627:therapeutic use*; D016232:Endothelins / Q000737:chemistry*; D006168:Guinea Pigs; D007548:Isosorbide Dinitrate / Q000627:therapeutic use*; D009569:Nitric Oxide / Q000378:metabolism*; D010047:Ovalbumin; D011897:Random Allocation
D001993:Bronchodilator Agents; D016232:Endothelins; D009569:Nitric Oxide; D010047:Ovalbumin; D007548:Isosorbide Dinitrate