title: Co-ordination between localized wound-induced Ca2+ signals and pre-wound serum signals is required for proliferation after mechanical injury.
abstract: The signals which initiate proliferation of endothelial cells after injury are important for selective blood vessel growth during wound healing or tumour growth. Upon mechanically wounding quiescent cells, a transient [Ca2+]i increase was induced in cells at the wound edge. These same cells proliferated 18-24 h post wounding, as measured by bromodeoxyuridine incorporation. The localized Ca2+ signal was required specifically during wounding since blocking Ca2+ influx reduced proliferation by 40-50%. Proliferation also required serum since starvation reduced proliferation by 80%. Serum-starved cells proliferated if briefly primed with serum prior to wounding. The signals derived from serum and [Ca2+]i combined at least additively to induce proliferation. Therefore, serum priming followed by a single, transient Ca2+ signal induced by mechanical injury must occur in a temporally and spatially regulated manner for normal proliferation. Co-ordination between signalling cascades induced by growth factors and release from contact inhibition might be obligatory for localized re-endothelialization after injury.
authors.affiliation: Pacific North-West Research Institute, Seattle, Washington, USA.
authors.forename: P O
authors.initials: PO
authors.lastname: Tran
authors.forename: Q H
authors.initials: QH
authors.lastname: Tran
authors.forename: L E
authors.initials: LE
authors.lastname: Hinman
authors.forename: P J
authors.initials: PJ
authors.lastname: Sammak
mesh_terms: D000818:Animals; D001790:Blood Physiological Phenomena; D001973:Bromodeoxyuridine / Q000378:metabolism; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D016895:Culture Media, Serum-Free; D004533:Egtazic Acid / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology*; D005682:Gadolinium / Q000494:pharmacology; D007399:Interphase; D015759:Ionomycin / Q000494:pharmacology; D008954:Models, Biological; D011651:Pulmonary Artery; D013997:Time Factors; D014945:Wound Healing / Q000502:physiology*
chemical_list: D002121:Calcium Channel Blockers; D016895:Culture Media, Serum-Free; D004533:Egtazic Acid; D015759:Ionomycin; D005682:Gadolinium; D001973:Bromodeoxyuridine; D002118:Calcium