title: Antigenic and nucleotide characterization of a herpesvirus isolated from Pacific harbor seals (Phoca vitulina richardsii).
abstract: This report describes the antigenic and nucleotide characterization of a herpes-like virus that has been isolated from the adrenal tissues of neonatal Pacific harbor seals. Infection with this virus has been previously implicated as a major cause of death of animals undergoing rehabilitation. Comparison and phylogenetic analysis of sequenced fragments of the DNA polymerase, glycoprotein B and glycoprotein D genes, and immunofluorescence assay using herpesvirus-specific monoclonal antibodies, demonstrated close similarity of the Pacific harbor seal herpesvirus to European isolates of phocid herpesvirus-1 (PHV-1) and other alpha-herpesviruses affecting terrestrial carnivores.
authors.affiliation: Marine Mammal Center, Sausalito, California, USA.
authors.forename: D P
authors.initials: DP
authors.lastname: King
authors.forename: R
authors.initials: R
authors.lastname: Parselles
authors.forename: F M
authors.initials: FM
authors.lastname: Gulland
authors.forename: J M
authors.initials: JM
authors.lastname: Lapointe
authors.forename: L J
authors.initials: LJ
authors.lastname: Lowenstine
authors.forename: D A
authors.initials: DA
authors.lastname: Ferrick
authors.forename: J L
authors.initials: JL
authors.lastname: Stott
mesh_terms: D000311:Adrenal Glands / Q000821:virology; D000595:Amino Acid Sequence; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001483:Base Sequence; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D010802:Phylogeny; D046048:Seals, Earless / Q000821:virology*
chemical_list: D000956:Antigens, Viral