The B29 (immunoglobulin beta-chain) gene is a genetic target for early B-cell factor.
Early B-cell factor (EBF) is a transcription factor suggested as essential for early B-lymphocyte development by findings in mice where the coding gene has been inactivated by homologous disruption. This makes the identification of genetic targets for this transcription factor pertinent for the understanding of early B-cell development. The lack of B29 transcripts, coding for the beta subunit of the B-cell receptor complex, in pro-B cells from EBF-deficient mice suggested that B29 might be a genetic target for EBF. We here present data suggesting that EBF interacts with three independent sites within the mouse B29 promoter. Furthermore, ectopic expression of EBF in HeLa cells activated a B29 promoter-controlled reporter construct 13-fold and induced a low level of expression from the endogenous B29 gene. Finally, mutations in the EBF binding sites diminished B29 promoter activity in pre-B cells while the same mutations did not have as striking an effect on the promoter function in B-cell lines of later differentiation stages. These data suggest that the B29 gene is a genetic target for EBF in early B-cell development.
Akerblad
P
P
Immunology Group, CMB, Lund University, S-223 62 Lund, Sweden.
Rosberg
M
M
Leanderson
T
T
Sigvardsson
M
M
D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D001402:B-Lymphocytes; D001483:Base Sequence; D001665:Binding Sites; D051925:CD79 Antigens; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D005803:Genes, Immunoglobulin; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D015703:Antigens, CD; D051925:CD79 Antigens; C497883:CD79B protein, human; C497884:Cd79b protein, mouse; D004268:DNA-Binding Proteins; C081976:EBF1 protein, human; C489921:Ebf1 protein, mouse; D015534:Trans-Activators; D014157:Transcription Factors