Familial hypertrophic cardiomyopathy mice display gender differences in electrophysiological abnormalities.
Genetically-manipulated mice harboring an alpha-myosin heavy chain Arg403Gln missense mutation (alpha-MHC403/+) display a phenotype characteristic of familial hypertrophic cardiomyopathy (FHC). Male and female (30 +/- 8 week old) heterozygous alpha-MHC403/+ mice and litter-mate controls were evaluated using a surface electrocardiogram (ECG) and an in vivo cardiac electrophysiology study (EPS). Wild type animals had normal intracardiac electrophysiology, with no significant differences between male and female control mice during EPS. The female wild-type mice did have slower heart rates and longer ECG intervals than their male wild-type counterparts. The female alpha-MHC403/+ mice had similar ECG's, cardiac conduction times, and refractory periods compared with female wild-type mice. In contrast, male FHC mice had distinctive ECG and electrophysiologic abnormalities including right axis deviation, prolonged ventricular repolarization and prolonged sinus node recovery times. During programmed ventricular stimulation, 62% of male alpha-MHC403/+ mice and 28% of female alpha-MHC403/+ mice had inducible ventricular tachycardia. These studies identify gender-specific electrophysiologic abnormalities in alpha-MHC403/+ FHC mice, concordant with the histological and hemodynamic derangements previously reported.
Berul
C I
CI
Department of Cardiology, Children's Hospital, Boston, MA 02115, USA. berul@cardio.tch.harvard.edu
Christe
M E
ME
Aronovitz
M J
MJ
Maguire
C T
CT
Seidman
C E
CE
Seidman
J G
JG
Mendelsohn
M E
ME
D000818:Animals; D001120:Arginine / Q000235:genetics; D002304:Cardiac Pacing, Artificial; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics* / Q000503:physiopathology; D016022:Case-Control Studies; D004562:Electrocardiography / Q000295:instrumentation / Q000379:methods; D005260:Female; D005973:Glutamine / Q000235:genetics; D006329:Heart Conduction System / Q000503:physiopathology; D006339:Heart Rate / Q000502:physiology; D006352:Heart Ventricles / Q000503:physiopathology; D006579:Heterozygote; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D020125:Mutation, Missense / Q000235:genetics; D018995:Myosin Heavy Chains / Q000235:genetics; D010641:Phenotype; D012032:Refractory Period, Electrophysiological / Q000235:genetics; D012737:Sex Factors; D012849:Sinoatrial Node / Q000503:physiopathology; D017180:Tachycardia, Ventricular / Q000209:etiology / Q000503:physiopathology
D005973:Glutamine; D001120:Arginine; D018995:Myosin Heavy Chains