Photoreactions of psoralens with lecithins.
The formation of cyclobutane (cb) photoadducts of psoralen with a model lecithin has been shown. The adducts are formed both in ethanol solution and in micellar suspension in water. In spite of their sensitivity to various factors such as light, temperature, air, etc., they are isolated by thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC) and characterized by mass spectrometry, NMR and UV absorption spectroscopy. The NMR analysis indicates the similarity of isomeric forms of cb adducts in lecithin to those formed with free oleic acid.
Zarebska
Z
Z
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland. zozar@ibb.waw.pl
Waszkowska
E
E
Caffieri
S
S
Dall'Acqua
F
F
D005363:Ficusin / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010713:Phosphatidylcholines / Q000378:metabolism*; D017319:Photosensitizing Agents / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013056:Spectrophotometry, Ultraviolet
D010713:Phosphatidylcholines; D017319:Photosensitizing Agents; D005363:Ficusin