title: Effects of acute brainstem compression on auditory brainstem response in the guinea pig.
abstract: BACKGROUND
  The purpose of this study was to establish the norm for parameters of auditory brainstem response (ABR) in the guinea pig and to investigate if acute brainstem compression results in significant changes to these parameters.
  
  
  METHODS
  Thirty-six guinea pigs with positive Preyer's reflex were anesthetized. A craniectomy was performed to remove the right occipital bone and the dura mater was opened to expose the brain, cerebellum and cerebellopontine angle (CPA). A small inflatable balloon was placed into the CPA precisely and slowly. ABR was recorded before incision of the skin as a baseline value, after placement and after inflation of the balloon with water at 0.1-ml intervals.
  
  
  RESULTS
  Five stable peaks were recorded in 27 experimental animals. When the balloon was inflated with 0.1 ml water, the absolute latency (AL) of peaks IV and V and the interpeak latency (IPL) of peaks III and IV, and IV and V were prolonged. The amplitude ratios (AR) of peaks II, III, IV and V to peak I decreased. Inflation of the balloon with 0.2 ml of water caused further elongation of ALs of peaks IV and V and decreases in each AR. When the balloon volume increased to 0.3 ml, peak V became unrecognizable and peaks III and IV showed significant elongation of AL; peaks I and II did not show significant change in ALs. Further increase of the balloon volume to 0.4 ml resulted in disappearance of peaks III, IV and V; AL of peak II was also elongated. However, the amplitude and AL of peak I remained unchanged. Similar changes were observed in IPLs.
  
  
  CONCLUSIONS
  This study establishes the norm of parameters of ABR in guinea pigs and demonstrates that acute brainstem compression causes elongation of ALs and IPLs of peaks II, III, IV and V. This suggests that peaks II, III, IV and V come from the brainstem and that peak I is not generated from the brainstem in the guinea pig.
authors.affiliation: Department of Otolaryngology, Veterans General Hospital-Taipei, Taiwan, ROC.
authors.forename: T Y
authors.initials: TY
authors.lastname: Tu
authors.forename: L H
authors.initials: LH
authors.lastname: Yu
authors.forename: J H
authors.initials: JH
authors.lastname: Chiu
authors.forename: C H
authors.initials: CH
authors.lastname: Shu
authors.forename: A S
authors.initials: AS
authors.lastname: Shiao
authors.forename: C F
authors.initials: CF
authors.lastname: Lien
mesh_terms: D000818:Animals; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D008297:Male; D011312:Pressure