title: Determination of common drugs of abuse in body fluids using one isolation procedure and liquid chromatography--atmospheric-pressure chemical-ionization mass spectromery.
abstract: A method for determining opiate agonists (morphine, morphine-3-glucuronide, morphine-6-glucuronide, 6-monoacetylmorphine, codeine, codeine-6-glucuronide, dihydrocodeine, dihydromorphine, buprenorphine, methadone, tramadol, and ibogaine), cocaine and its metabolites (benzoylecgonine and ecgonine methyl ester) and lysergic acid diethylamide in serum, blood, urine and other biological matrices is presented. Aliquots (0.5-1.5 mL) of biological fluids were spiked with appropriate deuterated internal standards and extracted using a common solid-phase extraction method (C18 cartridges). The extracts were subjected to liquid chromatographic-atmospheric-pressure chemical-ionization mass spectrometric examination using selected ion monitoring procedures. These procedures were developed after analysis of full-scan mass spectra of examined compounds. The extraction method appeared very universal; the recoveries were high for almost all drugs and the extracts were very clean. The procedure was applied for routine forensic casework.
authors.affiliation: Institute of Forensic Medicine, Aachen University of Technology, Germany.
authors.forename: M J
authors.initials: MJ
authors.lastname: Bogusz
authors.forename: R D
authors.initials: RD
authors.lastname: Maier
authors.forename: K D
authors.initials: KD
authors.lastname: Krüger
authors.forename: U
authors.initials: U
authors.lastname: Kohls
mesh_terms: D001826:Body Fluids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003042:Cocaine / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D013287:Illicit Drugs / Q000032:analysis*; D007118:Immunoassay; D066298:In Vitro Techniques; D008238:Lysergic Acid Diethylamide / Q000032:analysis / Q000097:blood / Q000652:urine; D009294:Narcotics / Q000032:analysis / Q000097:blood / Q000652:urine; D015203:Reproducibility of Results; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D013287:Illicit Drugs; D009294:Narcotics; D008238:Lysergic Acid Diethylamide; D003042:Cocaine