Terbium chelates for fluorescence immunoassays.
The sensitization of terbium(III) ion luminescence in the presence of 1-ethyl-14-dihydro-6,7-methylenedioxy-4-oxoquinoline-3-carboxylic (oxolinic) acid was studied. The terbium label is bound to the antibody with diethylenetriamine-pentaacetic acid anhydride (DTPAA). Optimum luminescence intensity is observed at pH 7.5 and the luminescence significantly increases in the presence of cationic surfactant, cetyltrimethylammonium bromide. The sensitivity of Tb(III) detection is 5 x 10(14) mol l(-1). This luminescence system is proposed for time-resolved fluoroimmunoassay.
Beltyukova
S V
SV
A.V. Bogatsky Physico-Chemical Institute of Academy of Sciences of Ukraine, Odessa.
Egorova
A V
AV
D002614:Chelating Agents / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005455:Fluorescent Antibody Technique; D007477:Ions; D008024:Ligands; D015394:Molecular Structure; D010093:Oxolinic Acid / Q000031:analogs & derivatives* / Q000276:immunology*; D013501:Surface-Active Agents / Q000737:chemistry; D013725:Terbium / Q000378:metabolism*
C118088:1-ethyl-1,4-dihydro-6,7-methylenedioxy-4-oxo-quinoline-3-carboxilic acid; D002614:Chelating Agents; D007477:Ions; D008024:Ligands; D013501:Surface-Active Agents; D013725:Terbium; D010093:Oxolinic Acid