Quantitative determination of butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone in oils, foods, and biological fluids by high-performance liquid chromatography with fluorometric detection.
Concentrations of synthetic antioxidants butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone were quantified using a high-performance liquid chromatograph with spectrofluorometric detector. The antioxidants were separated and eluted on a reversed-phase column by gradient of a mixture of H2O/acetonitrile/acetic acid (66.5: 28.5:5, by vol) and a mixture of acetonitrile/acetic acid (95:5, vol/vol). The eluants were monitored at emission and excitation wavelengths of 310 and 280 nm, respectively. Calibration curves obtained using peak areas against concentration showed high coefficients of multiple determination (R2 > 0.99) for all antioxidants. Known concentrations of added antioxidant standards were recoverable within 98-99% from oils and over 93% from mouse blood. This method requires minimum sample extraction and purification before analysis and provides a relatively high percentage recovery. The method has been applied successfully for the measurement of antioxidant concentrations in oils, dried foods, and biological fluids.
Yankah
V V
VV
Department of Food Science and Technology, Tokyo University of Fisheries, Japan.
Ushio
H
H
Ohshima
T
T
Koizumi
C
C
D000975:Antioxidants / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000379:methods; D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002084:Butylated Hydroxytoluene / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005396:Fish Products; D005470:Fluorometry; D005504:Food Analysis / Q000379:methods; D006873:Hydroquinones / Q000032:analysis*; D008461:Meat Products; D009821:Oils / Q000737:chemistry
D000975:Antioxidants; D006873:Hydroquinones; D009821:Oils; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C018855:2-tert-butylhydroquinone