title: Intact pancreatic islet function despite humoral xenorecognition in the pig-to-monkey combination.
abstract: BACKGROUND
  The aim of this study was to analyze humoral xenoreactivity of various Old World primate species sera against pig islets and the effects of these sera on pig islet viability and function after culture.
  
  
  METHODS
  Freshly isolated or cultured adult pig islets were analyzed by immunohistology or by cytofluorimetry for Old World primate xenoreactive natural antibody (XNA) binding and complement deposition. Complement-mediated cytotoxicity was evaluated by 51Cr release assays. After 4 days of culture in 50% sera from Old World primates, the morphology and in vitro metabolic function of pig islets were also analyzed.
  
  
  RESULTS
  Chimpanzee, Macaca mulatta (rhesus), or baboon XNA binding was detectable only on intra-islet endothelial cells (ECs). Incubation of pig islets with sera from all Old World primate species tested showed C3 and C4 deposition on ECs and on some surrounding endocrine cells. However, membrane attack complex (MAC) showed a pattern of positivity similar to XNA binding, i.e., restricted to ECs only. No deposition of factor B was detected. Although complement cascade was activated, no cytotoxicity was observed after incubation of islets with chimpanzee serum, whereas between 10% and 35% 51Cr specific release was obtained with rhesus, baboon, or Macaca fascicularis sera. Despite this cytotoxic effect, purified pig islets showed a normal morphology and a well-preserved insulin release in response to an acute glucose stimulus, after prolonged culture with 50% serum obtained from all primate species considered.
  
  
  CONCLUSIONS
  Despite the fact that pig beta-cell function was not affected by the serum of any of the primate species tested, some of them yielded significant lysis of islet cells, presumably as a result of a cytotoxic effect on intra-islet ECs. These data show that Old World primate sera from different species do not have equivalent effect on pig islets; these differences should be taken into account in preclinical trials of pig islet xenotransplantation.
authors.affiliation: Institut de la Santé et de la Recherche Médicale Unité 437 et Institut de Transplantation et Recherche en Transplantation, Nantes, France.
authors.forename: V
authors.initials: V
authors.lastname: Mirenda
authors.forename: B
authors.initials: B
authors.lastname: Le Mauff
authors.forename: F
authors.initials: F
authors.lastname: Boeffard
authors.forename: A
authors.initials: A
authors.lastname: Cassard
authors.forename: N
authors.initials: N
authors.lastname: Jugeau
authors.forename: J P
authors.initials: JP
authors.lastname: Soulillou
authors.forename: I
authors.initials: I
authors.lastname: Anegon
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000920:Antibody-Dependent Cell Cytotoxicity; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D005434:Flow Cytometry; D000882:Haplorhini; D007515:Islets of Langerhans / Q000166:cytology / Q000502:physiology*; D016381:Islets of Langerhans Transplantation / Q000276:immunology* / Q000502:physiology*; D013552:Swine; D014183:Transplantation, Heterologous / Q000276:immunology
chemical_list: D003470:Culture Media