title: Synthesis and antitumor evaluation of 2,5-disubstituted-indazolo[4, 3-gh]isoquinolin-6(2H)-ones (9-aza-anthrapyrazoles).
abstract: The synthesis and antitumor evaluation of 2, 5-disubstituted-indazolo[4,3-gh]isoquinolin-6(2H)-ones (9-aza-APs) are described. The key intermediates in the synthesis are benz[g]isoquinoline-5,10-diones which are substituted at positions 6 and 9 with groups of different nucleofugacity for SNAr displacements. The initial displacement of fluoride by a substituted hydrazine leads to the pyrazole analogues. Substitution of the remaining leaving group by an amine or BOC-protected amines leads to the 9-aza-APs 12. These analogues were converted into their maleate or hydrochloride salts 13. In two cases, namely, 13x and 13z, sidearm buildup was also employed in the synthetic pathway. In vitro evaluation of 9-aza-APs against the human colon tumor cell line LoVo uncovered for most of the compounds a cytotoxic potency lower than that of DuP-941 or mitoxantrone and comparable to that of doxorubicin. Only analogues 13c, 13n, and 13ff were as cytotoxic as DuP-941. Interestingly, while DuP-941 was highly cross-resistant in the LoVo cell line resistant to doxorubicin (LoVo/Dx), the 9-aza-APs carrying a distal lipophilic tertiary amine moiety in both chains were capable of overcoming the MDR resistance induced in this cell line. The 9-aza-APs show outstanding in vivo antitumor activity against both systemic P388 murine leukemia and MX-1 human mammary carcinoma transplanted in nude mice. At their optimal dosages, congeners 13a-c, 13f, 13n, 13q, 13x, and 13dd were highly effective against P388 leukemia with T/C% of 200-381, while the T/C% value of DuP-941 was 147. In the MX-1 tumor model, 24 compounds elicited percentages of tumor weight inhibitions (TWI) ranging from 50% to 99%. Congeners 13d, 13k, 13l, 13x, 13z, and 13ee emerged as the most effective ones, with TWI% 96, simliar to that of DuP-941 (TWI% = 95). On the basis of their efficacy profile in additional experimental tumors and lack of cardiotoxicity in preclinical models, two congeners have surfaced as potential clinical candidates.
authors.affiliation: Departments of Chemistry and Pharmacology, University of Vermont, Burlington, Vermont 05405, USA. pkrapcho@zoo.uvm.edu
authors.forename: A P
authors.initials: AP
authors.lastname: Krapcho
authors.forename: E
authors.initials: E
authors.lastname: Menta
authors.forename: A
authors.initials: A
authors.lastname: Oliva
authors.forename: R
authors.initials: R
authors.lastname: Di Domenico
authors.forename: L
authors.initials: L
authors.lastname: Fiocchi
authors.forename: M E
authors.initials: ME
authors.lastname: Maresch
authors.forename: C E
authors.initials: CE
authors.lastname: Gallagher
authors.forename: M P
authors.initials: MP
authors.lastname: Hacker
authors.forename: G
authors.initials: G
authors.lastname: Beggiolin
authors.forename: F C
authors.initials: FC
authors.lastname: Giuliani
authors.forename: G
authors.initials: G
authors.lastname: Pezzoni
authors.forename: S
authors.initials: S
authors.lastname: Spinelli
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000473:pathology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006801:Humans; D007191:Indazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D020128:Inhibitory Concentration 50; D007546:Isoquinolines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D007941:Leukemia P388 / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008325:Mammary Neoplasms, Experimental / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
chemical_list: C116720:2-(2-((2-hydroxyethyl)amino)ethyl)-5-((2-(dimethylamino)ethyl)amino)indazolo(4,3-gh)isoquinolin-6(2H)-one; C116721:2-(2-((2-hydroxyethyl)amino)ethyl)-5--((2-(methylamino)ethyl)amino)indazolo(4,3-gh)isoquinolin-6(2H)-one; D000970:Antineoplastic Agents; D007191:Indazoles; D007546:Isoquinolines