Imuthiol inhibits the etoposide-induced apoptosis in HL-60 cells.
Imuthiol (sodium-diethyl-dithiocarbamate, DTC) has been proven to possess immunomodulatory properties, this effect, however, varies in different model systems. This study was performed to assess the possible effect of imuthiol on etoposide-induced apoptosis in the human promyelocytic leukemia cell line, HL-60. In vitro pre-treatment with imuthiol at concentrations between 50 and 100 microM significantly inhibited etoposide-induced apoptosis of HL-60 cells, the inhibition appeared to be dose dependent. This effect may be associated with the immunomodulatory activity of imuthiol.
Nagy
E
E
Central Laboratory of Immunology, Semmelweis University, Budapest, Hungary.
Szakonyi
J
J
Gergely
P
P
D017209:Apoptosis / Q000187:drug effects*; D048429:Cell Size / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004050:Ditiocarb / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005047:Etoposide / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D005434:Flow Cytometry; D018922:HL-60 Cells; D006801:Humans; D013997:Time Factors; D020298:U937 Cells
D005047:Etoposide; D004247:DNA; D004050:Ditiocarb