Comparative genomic hybridization and its application to Wilms' tumorigenesis.
Eighty sporadic Wilms' tumor samples were analyzed by comparative genomic hybridization (CGH) to identify chromosomal regions involved in the etiology of the disease. Twenty percent of the samples showed chromosomal gains or losses. The majority of chromosomal gains and losses were similar to those identified through molecular and cytogenetic studies. Gains were observed on chromosomes 1q, 7q, 8, and 12, whereas losses were found on chromosomes 1p, 4p, 4q, 7p, 16q, 18q, 21q, and 22q. Other genetic aberrations identified in this study included deletions of chromosomes 5p and 15q, as well as gains of discrete loci on chromosomes 3p and 3q. These latter regions have not been previously implicated in Wilms' tumorigenesis and may contain novel genes relevant to the development and/or progression of this disease.
Getman
M E
ME
Department of Human Genetics, Genzyme Corporation, Framingham, MA (USA). mgetman@rics.bwh.harvard.edu
Houseal
T W
TW
Miller
G A
GA
Grundy
P E
PE
Cowell
J K
JK
Landes
G M
GM
D002869:Chromosome Aberrations; D004273:DNA, Neoplasm / Q000032:analysis; D017353:Gene Deletion; D015894:Genome, Human; D006801:Humans; D007091:Image Processing, Computer-Assisted; D017404:In Situ Hybridization, Fluorescence; D009693:Nucleic Acid Hybridization; D009396:Wilms Tumor / Q000235:genetics*
D004273:DNA, Neoplasm