Design of novel porphyrin-binding peptides based on antibody CDR.
Novel porphyrin-binding peptides were designed on the basis of an antigen binding site of an antiheme monoclonal antibody. Synthetic peptides were modified with a pyrene moiety. The spectroscopic measurements revealed that the synthetic peptides bound a porphyrin effectively.
Takahashi
M
M
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Ueno
A
A
Uda
T
T
Mihara
H
H
D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D007135:Immunoglobulin Variable Region / Q000737:chemistry*; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000378:metabolism; D011166:Porphyrins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007135:Immunoglobulin Variable Region; D010455:Peptides; D011166:Porphyrins; C018395:tetracarboxyphenylporphine