title: Differential regulation of interleukin-1alpha and interleukin-1beta in K-562 cells.
abstract: Interleukin (IL)-1alpha and IL-1beta are encoded by two separate genes, but both function as comitogens for lymphocyte activation. In this study, we observed K-562 cells to express constitutively mRNA for IL-1alpha, although IL-1alpha was not detected in the growth-conditioned medium (GCM). However, IL-1beta mRNA was not expressed unless the cells had been treated with phorbol myristate acetate (PMA). Both IL-1alpha and IL-1beta were detected in the GCM after the cells had been cultured with PMA, suggesting that IL-1 elaboration required PMA treatment. The K-562 cells treated with PMA differentiated to the myeloblastic stage, as observed by nuclear morphologic properties by electron microscopy. PMA treatment induced de novo expression of CD61 or gpIIIa, a marker associated with megakaryoblasts. These results showed that although K-562 cells constitutively expressed IL-1alpha mRNA, PMA treatment was required for secretion. On the other hand, both the expression and secretion of IL-1beta required treatment with PMA. This study showed that K-562 cells treated with PMA differentiated to the myeloblastic stage and expressed and secreted IL-1alpha and IL-1beta.
authors.affiliation: Graduate School of Medicine, University of Tennessee Medical Center at Knoxville, 37920, USA. aichiki@mc.utmck.edu
authors.forename: A T
authors.initials: AT
authors.lastname: Ichiki
authors.forename: M
authors.initials: M
authors.lastname: Langenberg
authors.forename: E J
authors.initials: EJ
authors.lastname: Baker
authors.forename: J W
authors.initials: JW
authors.lastname: Hodge
authors.forename: E G
authors.initials: EG
authors.lastname: Bamberger
authors.forename: D A
authors.initials: DA
authors.lastname: Gerard
authors.forename: C B
authors.initials: CB
authors.lastname: Lozzio
mesh_terms: D002455:Cell Division / Q000187:drug effects; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014407:Tumor Cells, Cultured
chemical_list: D007375:Interleukin-1; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate