In vivo quantitation of epithelial lining fluid in dog lung.
We used an original saturation bronchoalveolar lavage (SBAL) technique (Eur. Respir. J. 1995;8[Suppl. 19]398S) to quantitate lung epithelial lining fluid volume (VELF) in dogs in two separate experiments: control and after oleic-acid-induced injury. We confirmed the hypothesis that 99mTc-DTPA, infused at constant plasma activity, reaches equilibrium with epithelial lining fluid after 90 min. We performed eight sequential lavages 215 min after beginning the infusion of 99mTc-DTPA. 99mTc-DTPA activity (Qn) in the lavage fluid increased linearly with time, suggesting transport from the plasma into the alveoli during lavage. We extrapolated Qn to time zero (Q0), when 99mTc-DTPA was not affected by lavage. VELF was calculated from: VELF = Q0/Cp, (Cp: 99mTc-DTPA mean plasma activity). 125I-albumin was used as a nondiffusible alveolar indicator to measure the fluid volume present in the lavaged segment (Vt,n). Vt,n plateaud for n >= 4. VELF/Vt,n(n = 5,8) was 1.7 +/- 0.4 and 25.0 +/- 4.4% (p < 0.05) in control and injury experiments, respectively. SBAL allowed reliable measurements of VELF and detection of alveolar edema fluid in the injured lung.
Bayat
S
S
Unité Mixte de Recherche 5525 du Centre National de Recherche Scientifique, Service de Médecine Nucléaire, Centre Hospitalier Universitaire de Grenoble, France.
Menaouar
A
A
Anglade
D
D
Ettinger
H
H
François-Joubert
A
A
Benchetrit
G
G
Grimbert
F A
FA
D000418:Albumins / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure; D018893:Bronchoalveolar Lavage / Q000379:methods; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004285:Dogs; D004848:Epithelium / Q000378:metabolism; D015633:Extravascular Lung Water / Q000737:chemistry; D005122:Exudates and Transudates / Q000737:chemistry; D007262:Infusions, Intravenous; D007457:Iodine Radioisotopes; D008168:Lung / Q000378:metabolism*; D010100:Oxygen / Q000097:blood; D011650:Pulmonary Alveoli / Q000378:metabolism; D011654:Pulmonary Edema / Q000378:metabolism; D011669:Pulmonary Wedge Pressure; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000097:blood; D012044:Regression Analysis; D015203:Reproducibility of Results; D012128:Respiratory Distress Syndrome / Q000378:metabolism; D012709:Serum Albumin / Q000032:analysis; D016284:Technetium Tc 99m Pentetate / Q000008:administration & dosage / Q000097:blood; D013997:Time Factors
D000418:Albumins; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D012709:Serum Albumin; D010100:Oxygen; D016284:Technetium Tc 99m Pentetate