Detection of bovine milk proteins in soymilk by Western blotting.
A Western blotting method for the detection of whey milk proteins in commercial soymilks was applied to assess the food safety. Soy proteins and milk proteins were separated by SDS-PAGE in PhastSystem equipment. After the electrophoretic separation, immunodetection with anti-bovine alpha-lactalbumin and anti-bovine beta-lactoglobulin antisera was performed. Adulteration with bovine protein in percentages of 0.1% in soy protein can be detected. Western blotting of bovine alpha-lactalbumin and bovine beta-lactoglobulin was applied to detect adulteration by bovine milk proteins in different soymilks: powdered soymilk and soy infant formulas.
Molina
E
E
Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain.
Amigo
L
L
Ramos
M
M
D000818:Animals; D015153:Blotting, Western; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005506:Food Contamination / Q000517:prevention & control; D005513:Food Inspection / Q000379:methods* / Q000592:standards; D007225:Infant Food / Q000032:analysis*; D008894:Milk Proteins / Q000032:analysis*; D030262:Soybean Proteins / Q000032:analysis; D013025:Glycine max / Q000378:metabolism*
D008894:Milk Proteins; D030262:Soybean Proteins