title: Comparison of two immunoradiometric assays for parathyroid hormone-related protein in the evaluation of cancer patients with and without hypercalcemia.
abstract: Hypercalcemia is a common paraneoplastic syndrome due to the secretion by tumors of parathyroid hormone-related protein (PTHrP) and/or other osteolytic factors. In the present study, we have measured plasma PTHrP using two immunoradiometric assays for PTHrP, assay N (Nichols) and assay I (INCSTAR), recognizing the 1-86 domain of PTHrP, for the evaluation of malignancy-associated hypercalcemia. The study included 25 tumor patients with hypercalcemia (HCa) [corrected serum calcium (SCa) > or = 2.70 mmol/L], 20 normocalcemic patients with cancer (NCa), and ten healthy control subjects. Plasma PTHrP was either undetectable or within the respective normal range in the majority of NCa patients and in the control subjects, with both assays. Plasma PTHrP was increased in 13 and 15 of HCa cases with assay N and assay I, respectively. PTHrP was elevated in plasma in 5/6 (assay N) and 3/6 (assay I) HCa patients with squamous tumors. However, plasma PTHrP was high in only 2/9 (assay N) and 1/9 (assay I) HCa cases with hematological tumors. Less than 40% of HCa patients with bone metastases, and >75% of those without bone involvement, had elevated plasma PTHrP with both assays. Detectable plasma PTHrP and SCa were significantly correlated using assay N (p = 0.025) and assay I (p = 0.01), in the HCa group. A highly significant correlation (p <0.001) was found between detectable plasma PTHrP with both assays, and a high agreement between them based on simple kappa statistics (p < 0.001), in the latter group. Our results indicate that each assay may be similarly useful in detecting PTHrP hyperproduction in cancer patients.
authors.affiliation: Research Unit, Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: de Miguel
authors.forename: J L
authors.initials: JL
authors.lastname: Motellón
authors.forename: J
authors.initials: J
authors.lastname: Hurtado
authors.forename: F J
authors.initials: FJ
authors.lastname: Jiménez
authors.forename: P
authors.initials: P
authors.lastname: Esbrit
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001859:Bone Neoplasms / Q000556:secondary; D016022:Case-Control Studies; D005069:Evaluation Studies as Topic; D006801:Humans; D006934:Hypercalcemia / Q000097:blood* / Q000150:complications; D015592:Immunoradiometric Assay / Q000379:methods*; D008875:Middle Aged; D009369:Neoplasms / Q000097:blood* / Q000150:complications; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: C470121:PTHLH protein, human; D044162:Parathyroid Hormone-Related Protein; D011506:Proteins