A novel autoantibody in paraneoplastic sensory-dominant neuropathy reacts with brain-type creatine kinase.
We have previously reported on a novel autoantibody in a patient with paraneoplastic sensory-dominant neuropathy. This autoantibody immunostains the rat primary sensory system and reacts with a 47 kDa protein on immunoblotting. Here, we report on the isolation from rat spinal cord of a molecule that is recognized by this autoantibody. By ammonium sulfate cut and gel filtration, affinity and ion exchange chromatographies, the immunoreactive protein was purified to homogeneity and identified as brain-type creatine kinase (B-CK). Our study revealed that the autoantibody of the patient reacted with B-CK in the primary sensory system.
Arawaka
S
S
Third Department of Internal Medicine, Yamagata University School of Medicine, Yamagata 990-23, Japan.
Daimon
M
M
Sasaki
H
H
Suzuki
J I
JI
Kato
T
T
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D001323:Autoantibodies / Q000276:immunology*; D001921:Brain / Q000201:enzymology*; D003402:Creatine Kinase / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009422:Nervous System Diseases / Q000276:immunology*; D010257:Paraneoplastic Syndromes / Q000276:immunology*; D051381:Rats; D013116:Spinal Cord / Q000737:chemistry / Q000201:enzymology
D001323:Autoantibodies; D003402:Creatine Kinase