title: Inhibition of prostanoid synthesis protects against neuronal damage induced by focal ischemia in rat brain.
abstract: Changes in prostanoids concentration and effects of the non-specific COX inhibitor indomethacin on prostanoids levels and extension of tissue damage were studied following focal ischemia induction in the fronto-parietal region of rat brain. Ischemia was induced in animals bearing a transcerebral microdialysis probe by injection of Rose Bengal, a photosensitive dye, followed by light activation. Prostanoid levels were determined in the dialysate using immunoenzymatic techniques. PGD2 levels rose significantly up to 237+/-22 pg/ml compared to a basal level measured before ischemia induction which was below the detection limit. TXB2 changes were smaller and had a different time course. Treatment with indomethacin abolished the ischemia-induced PGD2 release and reduced the extent of injury to the area by 43+/-3.7%. These results suggest that prostanoid release may play an important role in neurodegenerative processes and that cyclooxygenase inhibitors may contribute to protect against cerebral tissue damage.
authors.affiliation: Institute of Pharmacological Sciences, University of Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Buccellati
authors.forename: G C
authors.initials: GC
authors.lastname: Folco
authors.forename: A
authors.initials: A
authors.lastname: Sala
authors.forename: R
authors.initials: R
authors.lastname: Scelsi
authors.forename: E
authors.initials: E
authors.lastname: Masoero
authors.forename: P
authors.initials: P
authors.lastname: Poggi
authors.forename: S
authors.initials: S
authors.lastname: Govoni
authors.forename: L
authors.initials: L
authors.lastname: Favalli
authors.forename: A
authors.initials: A
authors.lastname: Rozza
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000517:prevention & control; D002546:Ischemic Attack, Transient / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology* / Q000648:ultrastructure; D011448:Prostaglandin Antagonists / Q000627:therapeutic use; D011453:Prostaglandins / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011448:Prostaglandin Antagonists; D011453:Prostaglandins