Construction and physiological analysis of a Xanthomonas oryzae pv. oryzae recA mutant.
A Xoo recA insertion inactivation mutant was constructed. The mutant, lacking RecA, showed increased sensitivity towards mutagen killing. This phenotype could be complemented by a cloned, functional recA. Unlike other bacteria, both the recA mutant and the parental strain had similar level of resistance to H2O2 killing and peroxide-induced mutagenesis.
Mongkolsuk
S
S
Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok, Thailand. scsmk@mucc.mahidol.ac.th
Rabibhadana
S
S
Sukchavalit
R
R
Vaughn
G
G
D015139:Blotting, Southern; D015153:Blotting, Western; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016254:Mutagenesis, Insertional; D016133:Polymerase Chain Reaction; D011938:Rec A Recombinases / Q000235:genetics*; D013997:Time Factors; D014974:Xanthomonas / Q000187:drug effects / Q000235:genetics* / Q000502:physiology
D006861:Hydrogen Peroxide; D011938:Rec A Recombinases