Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R.
The type IIA rat brain sodium channel is composed of three subunits: a large pore-forming alpha subunit and two smaller auxiliary subunits, beta1 and beta2. The beta subunits are single membrane-spanning glycoproteins with one Ig-like motif in their extracellular domains. The Ig motif of the beta2 subunit has close structural similarity to one of the six Ig motifs in the extracellular domain of the cell adhesion molecule contactin (also called F3 or F11), which binds to the extracellular matrix molecules tenascin-C and tenascin-R. We investigated the binding of the purified sodium channel and the extracellular domain of the beta2 subunit to tenascin-C and tenascin-R in vitro. Incubation of purified sodium channels on microtiter plates coated with tenascin-C revealed saturable and specific binding with an apparent Kd of approximately 15 nM. Glutathione S-transferase-tagged fusion proteins containing various segments of tenascin-C and tenascin-R were purified, digested with thrombin to remove the epitope tag, immobilized on microtiter dishes, and tested for their ability to bind purified sodium channel or the epitope-tagged extracellular domain of beta2 subunits. Both purified sodium channels and the extracellular domain of the beta2 subunit bound specifically to fibronectin type III repeats 1-2, A, B, and 6-8 of tenascin-C and fibronectin type III repeats 1-2 and 6-8 of tenascin-R but not to the epidermal growth factor-like domain or the fibrinogen-like domain of these molecules. The binding of neuronal sodium channels to extracellular matrix molecules such as tenascin-C and tenascin-R may play a crucial role in localizing sodium channels in high density at axon initial segments and nodes of Ranvier or in regulating the activity of immobilized sodium channels in these locations.
Srinivasan
J
J
Departments of Pharmacology and Neurological Surgery, University of Washington, Seattle, WA 98195-7280, USA.
Schachner
M
M
Catterall
W A
WA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000502:physiology*; D007700:Kinetics; D046911:Macromolecular Substances; D008958:Models, Molecular; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D015222:Sodium Channels / Q000737:chemistry / Q000502:physiology*; D019063:Tenascin / Q000737:chemistry / Q000502:physiology*
D015815:Cell Adhesion Molecules; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D015222:Sodium Channels; D019063:Tenascin; C074638:tenascin R