title: Rapid enzyme immunoassay for measurement of bovine progesterone.
abstract: Reproductive management is a primary financial concern of the dairy industry with missed estrus detection one of the major causes of lost income. A rapid enzyme immunoassay (EIA) was developed for on-line measurement of progesterone in bovine milk with a biosensor for detection of estrus. The EIA was developed using covalent binding microtiter wells, monoclonal antibody, horseradish peroxidase, and 3,3',5,5'-tetramethylbenzidine (TMB). The EIA took 8 min and had a dynamic response for progesterone in buffer and milk between 0.2 and 20 ng/ml.
authors.affiliation: DDx, Incorporated, Denver, CO 80221, USA.
authors.forename: R W
authors.initials: RW
authors.lastname: Claycomb
authors.forename: M J
authors.initials: MJ
authors.lastname: Delwiche
authors.forename: C J
authors.initials: CJ
authors.lastname: Munro
authors.forename: R H
authors.initials: RH
authors.lastname: BonDurant
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques; D002417:Cattle; D007124:Immunoenzyme Techniques; D008892:Milk / Q000737:chemistry*; D011374:Progesterone / Q000032:analysis* / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D011374:Progesterone