title: A novel spliced transcript of influenza A/WSN/33 virus.
abstract: It has been shown that influenza A virus M1 mRNA has two alternative 5" splice sites: a distal 5" splice site producing M3 mRNA that has the coding potential for 9 amino acids and a proximal 5" splice site producing M2 mRNA that encodes the essential M2 ion-channel protein. In this study, we demonstrated that the laboratory widely used strain A/WSN/33, but not A/Udorn/72 possessed another novel 5" splice site producing a transcript with the coding potential for 54 amino acids. We nominated this novel transcript as M4 mRNA. M4 mRNA was detected in A/WSN/33-infected cells derived from different species. Sequence comparison of M1 mRNA in both A/WSN/33 and Udorn/72 at position 143 to 151 reveals that this novel 5" splice site generated in WSN was due to one nucleotide difference at position 147. Several strains of influenza A viruses other than WSN also possess the potential M4 5" splice site by sequence analysis from the files of GenBank.
authors.affiliation: School of Medical Technology, Chang Gung University, Taiwan, ROC.
authors.forename: S R
authors.initials: SR
authors.lastname: Shih
authors.forename: P C
authors.initials: PC
authors.lastname: Suen
authors.forename: Y S
authors.initials: YS
authors.lastname: Chen
authors.forename: S C
authors.initials: SC
authors.lastname: Chang
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014709:Vero Cells
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral