N-[11C]methylpiperidine esters as acetylcholinesterase substrates: an in vivo structure-reactivity study.
A series of simple esters incorporating the N-[11C]methylpiperidine structure were examined as in vivo substrates for acetylcholinesterase in mouse brain. 4-N-[11C]Methylpiperidinyl esters, including the acetate, propionate and isobutyrate esters, are good in vivo substrates for mammalian cholinesterases. Introduction of a methyl group at the 4-position of the 4-piperidinol esters, to form the ester of a teritary alcohol, effectively blocks enzymatic action. Methylation of 4-N-[11C]methylpiperidinyl propionate at the 3-position gives a derivative with increased in vivo reactivity toward acetylcholinesterase. Esters of piperidinecarboxylic acids (nipecotic, isonipecotic and pipecolinic acid ethyl esters) are not hydrolyzed by acetylcholinesterase in vivo, nor do they act as in vivo inhibitors of the enzyme. This study has identified simple methods to both increase and decrease the in vivo reactivity of piperidinyl esters toward acetylcholinesterase.
Kilbourn
M R
MR
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor 48109, USA. mkilbour@umich.edu
Nguyen
T B
TB
Snyder
S E
SE
Sherman
P
P
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000201:enzymology*; D002250:Carbon Radioisotopes; D004952:Esters; D007700:Kinetics; D051379:Mice; D010880:Piperidines / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D051381:Rats; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014055:Tomography, Emission-Computed; D015899:Tomography, Emission-Computed, Single-Photon
D002250:Carbon Radioisotopes; D004952:Esters; D010880:Piperidines; D019275:Radiopharmaceuticals; D000110:Acetylcholinesterase