Rapid and convenient method of autoradiography for DNA cloning using digital imaging analysis.
Digital image analysis has been used for various biochemical and molecular biological analyses instead of autoradiography with X-ray film. However, in such cases the data manipulated by an imaging analyzer was generally printed out on normal printing paper. With normal paper, it is difficult to align the signal or its position relative to the original sample. Here in, we demonstrate it to be convenient and accurate to align signal obtained by imaging analyzer with OHP film.
Taketani
Y
Y
Department of Clinical Nutrition, University of Tokushima School of Medicine, Japan.
Chikamori
M
M
Tanaka
K
K
Yamamoto
H
H
Morita
K
K
Miyamoto
K
K
Takeda
E
E
D001345:Autoradiography / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods*; D004274:DNA, Recombinant / Q000032:analysis*; D015723:Gene Library; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D012815:Signal Processing, Computer-Assisted; D014962:X-Ray Film
D004274:DNA, Recombinant