title: Comparison of HPLC, TLC and minicolumn for measuring the radiochemical purity of 99Tcm-Q12.
abstract: TechneScan Q12 (99Tcm-Q12) is a new agent for clinical myocardial perfusion imaging. A product with high radiochemical purity is essential for high-quality imaging. We compared three methods of radiochemical purity analysis for 99Tcm-Q12: thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and minicolumn. Thin-layer chromatography resulted in lower purity (87 +/- 8%) than the minicolumn method (aluminium oxide minicolumn, ethanol) (95 +/- 4%). The HPLC method resulted in the lowest purity (79 +/- 11%). The main impurity was a polar compound, but three further impurities were found using HPLC. Using HPLC, we found the percentage of the parent compound (99Tcm-Q12) in plasma at 40-90 min post-injection to be 19.3 +/- 6.2%. We suggest that gradient HPLC is the most effective method for the analysis of the radiochemical purity of 99Tcm-Q12, and that it can be used to determine the concentration of 99Tcm-Q12 in plasma.
authors.affiliation: Department of Clinical Physiology and Nuclear Medicine, Kuopio University Hospital, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Yu
authors.forename: K A
authors.initials: KA
authors.lastname: Bergström
authors.forename: J T
authors.initials: JT
authors.lastname: Kuikka
authors.forename: J
authors.initials: J
authors.lastname: Yang
authors.forename: E
authors.initials: E
authors.lastname: Vanninen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004340:Drug Contamination; D007202:Indicators and Reagents; D009943:Organophosphorus Compounds / Q000032:analysis*; D015609:Organotechnetium Compounds / Q000032:analysis*; D019275:Radiopharmaceuticals / Q000032:analysis*
chemical_list: D007202:Indicators and Reagents; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C114153:technescan Q12