A simple procedure for solid-phase synthesis of peptide nucleic acids with N-terminal cysteine.
Problems were encountered during attempts to prepare N-terminal cysteine-substituted peptide nucleic acids (PNAs) from commercially available, Fmoc-protected monomers. These problems have been surmounted by the use of an S-t-butylmercapto protecting group on the cysteine moiety. The solid-phase syntheses are carried out via a simplified procedure which should be generally useful for manual PNA synthesis.
Goodwin
T E
TE
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
Holland
R D
RD
Lay
J O
JO
Raney
K D
KD
D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D012116:Resins, Plant; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D020135:Peptide Nucleic Acids; D012116:Resins, Plant; D003545:Cysteine