[Screening of thermophilic superoxide dismatase producing strain and some properties of the enzyme].
A thermophilic SOD producing strain was obtained from the bacteria preserved in our lab. Its content of SOD was 8774u/g fresh cells. The strain can tolerate 0.4% H2O2 and 70 degrees C, and it has outstanding culture characteristics. It was identified as Bacillus stearothermophilus, called B.S 211-15. Crude SOD was extracted from B.S 211-15, the recovery of total activity was 69.5%, the specific activity was 1793u/mg protein. The enzyme showed fine heat stability, pH stability and good proteinase resistance. The SOD activity didn't decrease after being kept in room temperature for 2 months. The results of inhibition reactions indicated that this enzyme was Fe-SOD.
Wang
Z
Z
Department of Biotechnology, Sichuan University, Chengdu.
Huang
Y
Y
Hu
Y
Y
Hu
P
P
D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000302:isolation & purification; D013482:Superoxide Dismutase / Q000302:isolation & purification* / Q000378:metabolism
D013482:Superoxide Dismutase