title: Effect of procainamide on pulmonary thromboembolism and platelet malondialdehyde in mice.
abstract: OBJECTIVE
  To study the effect of procainamide (PA) on pulmonary thromboembolism, platelet malondialdehyde (MDA) level and platelet aggregation induced by collagen and adrenaline.
  
  
  METHODS
  Pulmonary thromboembolism, 2-thiobarbituric acid fluorescence micro-determination, conventional microscopic counting, and platelet aggregation test were used.
  
  
  RESULTS
  PA (10-20 mg.kg-1) and mannitol (200 mg.kg-1) reduced thrombosis by 30%-75% and 75%, respectively. Thrombocytopenia followed thrombosis increased after the pretreatment of PA and mannitol. MDA decreased by both of them in vivo. In vitro, PA inhibited platelet aggregation and MDA production induced concentration-dependently by collagen and adrenaline.
  
  
  CONCLUSIONS
  Inhibition of PA on pulmonary thromboembolism is involved in the decrease of platelet aggregation and MDA production.
authors.affiliation: Department of Pharmacology, First Military Medical University, Guangzhou, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Pang
authors.forename: L S
authors.initials: LS
authors.lastname: Sun
authors.forename: X Y
authors.initials: XY
authors.lastname: Wang
authors.forename: S Q
authors.initials: SQ
authors.lastname: Yang
authors.forename: C W
authors.initials: CW
authors.lastname: Shan
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000378:metabolism; D003094:Collagen; D004837:Epinephrine; D008297:Male; D008315:Malondialdehyde / Q000097:blood*; D051379:Mice; D010974:Platelet Aggregation / Q000187:drug effects; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011342:Procainamide / Q000494:pharmacology*; D011655:Pulmonary Embolism / Q000097:blood* / Q000139:chemically induced
chemical_list: D010975:Platelet Aggregation Inhibitors; D008315:Malondialdehyde; D003094:Collagen; D011342:Procainamide; D004837:Epinephrine