[Effect of matrix metalloproteinase inhibitor on experimental pseudomonal corneal ulceration].
We examined the effect of matrix metalloproteinase (MMP) inhibitor (CS-610) on experimental pseudomonal corneal ulceration by clinical and histological evaluation. Intrastromal injection of 3.5 microliters sterile culture broth of P. aeruginosa, IID-1117 (13.5 unit Type I collagenase equivalent proteinase activities), was done to induce corneal ulcers in guinea pigs. The animals were divided into two groups of 23 each. The CS-610 group received topical CS-610 (400 micrograms/ml) treatment at 2-hour intervals and the control group received only the vehicle of CS-610 at the same intervals. In the control group, corneas developed acute corneal damage following corneal ulcerations at 6-12 hours. In the CS-610 group, these corneal lesions were inhibited in most of the eyes (p < 0.01). In the late period, as inflammatory cells migrated into the cornea, some animals of the CS-610 group developed corneal ulcer. The results indicated that CS-610 had a potent inhibitory activity against pseudomonal proteinases in vivo. The results also suggested that the mechanism of the ulceration model involved not only pseudomonal proteinases but also endogenous responses.
Kosaku
K
K
Department of Ophthalmology, Nihon University School of Medicine, Tokyo, Japan.
Maeda
N
N
Yokoyama
T
T
Inoue
T
T
Yoshida
Y
Y
Hosokawa
T
T
Sawa
M
M
D000818:Animals; D003315:Cornea / Q000473:pathology; D003320:Corneal Ulcer / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D006168:Guinea Pigs; D007211:Indoles / Q000627:therapeutic use*; D007634:Keratitis / Q000150:complications / Q000382:microbiology*; D008297:Male; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors*; D011552:Pseudomonas Infections
C112083:CS 610; D004791:Enzyme Inhibitors; D007211:Indoles; D008666:Metalloendopeptidases