Anti-inflammatory activity of D-002: an active product isolated from beeswax.
D-002 is a natural mixture of high molecular weight alcohols isolated and purified from beeswax, which contains triacontanol among its main components. This study was undertaken to investigate the anti-inflammatory effects of D-002 administered by the oral route in two animal models commonly used in the pharmacological screening of anti-inflammatory drugs. D-002 administered orally to rats (100 and 200 mg/kg) produced a mild but significant reduction of exudate volume in carrageenan-induced pleuritic inflammation that was accompanied by a marked and significant decrease of leukotriene B4 (LTB4) levels in the exudate. D-002 (25, 50 and 200 mg/kg) also significantly diminished the granuloma weight in the cotton pellet granuloma in rats. In both cases, D-002 was less effective than indomethacin, which was used as an established anti-inflammatory reference drug. On the other hand, D-002 administered from 25-1000 mg/kg did not induce erosions or gastromucosal lesions in rats, which differs from results usually obtained with non steroidal anti-inflammatory drugs. These results indicate that D-002 is a mild anti-inflammatory agent without any ulcerogenic effect associated. The results suggest that these effects are probably not mediated through an inhibition of cyclooxygenase, but a reduction in LTB4 levels induced by D-002 could explain these results.
Carbajal
D
D
Center of Natural Products National Center for Scientific Research, C. Habana, Cuba.
Molina
V
V
Valdés
S
S
Arruzazabala
M L
ML
Más
R
R
Magraner
J
J
D000284:Administration, Oral; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000627:therapeutic use; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use; D002351:Carrageenan / Q000494:pharmacology; D005233:Fatty Alcohols / Q000494:pharmacology* / Q000627:therapeutic use; D005753:Gastric Mucosa / Q000187:drug effects; D003368:Gossypium / Q000378:metabolism; D006099:Granuloma / Q000139:chemically induced / Q000188:drug therapy; D007213:Indomethacin / Q000494:pharmacology / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014885:Waxes / Q000737:chemistry*
D000893:Anti-Inflammatory Agents; D000897:Anti-Ulcer Agents; C104511:D 002; D005233:Fatty Alcohols; D014885:Waxes; C038228:beeswax; D002351:Carrageenan; D007213:Indomethacin