Antitumor activity of the aqueous-alcoholic extracts from unripe cotton ball of Gossypium indicum.
The present study investigated the antitumor activity of the aqueous-alcoholic extracts from unripe cotton balls of Gossypium indicum. An Exposure of murine B16 melanoma and L1210 lymphoma cells to the extracts resulted in their severe deaths in time- and concentration-dependent manners. Of the extracts, hydrophilic fractions were most efficacious for the antitumor activity and found to contain certain amounts of catechin and its derivatives. The hydrophilic extract fraction C36B2-8 had approximately 10 times more cytotoxic effects on B12 and L1210 cells than on isolated murine thymocytes. High concentrations (> 150 micrograms/ml) of C 36B3-8 mainly induced necrotic cell death. At low concentrations (< 100 micrograms/ml), however, C 36B3-8 induced not only necrosis but also apoptosis of the two tumor cell lines, which was proved by the TUNEL staining and DNA fragmentation techniques. The data indicate that certain ingredients of the cotton ball extract of G. indicum have an antitumor activity.
Choi
J J
JJ
College of Medicine, Ewha Womans University, Seoul, Korea.
Yoon
K N
KN
Lee
S K
SK
Lee
Y H
YH
Park
J H
JH
Kim
W Y
WY
Kim
J K
JK
Kim
W K
WK
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D004396:Coloring Agents; D053938:DNA Fragmentation / Q000187:drug effects; D004273:DNA, Neoplasm / Q000187:drug effects; D003368:Gossypium / Q000737:chemistry*; D007928:Lethal Dose 50; D007939:Leukemia L1210 / Q000188:drug therapy; D008546:Melanoma, Experimental / Q000188:drug therapy; D051379:Mice; D008813:Mice, Inbred ICR; D010936:Plant Extracts / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000187:drug effects; D014343:Trypan Blue; D014407:Tumor Cells, Cultured
D000972:Antineoplastic Agents, Phytogenic; D004396:Coloring Agents; D004273:DNA, Neoplasm; D010936:Plant Extracts; D014343:Trypan Blue