Dietary specific sugars for serum protein enzymatic glycosylation in man.
All glycoprotein sugars can theoretically derive from glucose. However, dietary specific sugars could represent preferential substrates or have regulatory roles in enzymatic glycosylation. This hypothesis was tested in man using stable isotopes. Healthy subjects ingested different amounts (150, 300, or 550 mg) of artificially 13C-enriched sugar (galactose, mannose, or glucose) diluted in 200 mL water containing 50 g 13C-poor sucrose. 13C enrichment of expired CO2 was monitored for 8 hours during indirect calorimetry. Serum glycoproteins were precipitated and delipidated at various intervals. Glycoprotein neutral sugars were obtained by acidic hydrolysis, purified by ion-exchange chromatography, derivatized to alditol acetates, and analyzed by gas chromatography-isotope ratio mass spectrometry. The oxidation rate for galactose and mannose was slower than the rate for glucose. Total oxidation over the 8-hour period was less than 10% of the ingested amount of galactose or mannose. Galactose and mannose were readily incorporated into glycoprotein glycans, in the native form or after interconversion, despite ingestion of a large excess of sucrose: glycoprotein sugar 13C enrichment was strongly higher after 13C-galactose or 13C-mannose than after 13C-glucose. Thus, the metabolism of these three sugars appears to be different. Specific dietary sugars could represent a new class of non essential nutrients displaying interesting metabolic roles. This could have practical consequences especially in parenteral nutrition, where glucose is currently the only sugar available for metabolism.
Berger
V
V
Institut National de la Santé et de la Recherche Medicale-Centre National de la Recherche Scientifique, U189, Lyon-Sud Medical School, Oullins, France.
Périer
S
S
Pachiaudi
C
C
Normand
S
S
Louisot
P
P
Martin
A
A
D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis; D004032:Diet; D005260:Female; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000097:blood*; D006031:Glycosylation; D006801:Humans; D008297:Male; D008358:Mannose / Q000378:metabolism; D009005:Monosaccharides / Q000378:metabolism*
D001798:Blood Proteins; D002247:Carbon Isotopes; D006023:Glycoproteins; D009005:Monosaccharides; D002245:Carbon Dioxide; D005947:Glucose; D008358:Mannose; D005690:Galactose