title: Calretinin and calbindin D28K immunoreactivity in the superficial layers of the rabbit superior colliculus.
abstract: Calretinin and calbindin D28K were localized in the superficial layers of rabbit superior colliculus (SC). Calretinin and calbindin D28K-immunoreactive (-IR) neurons were concentrated in the upper superficial gray layer. Calretinin-IR fibers were found in the optic layer. The majority of calretinin-IR cells were small- to medium-sized vertical fusiform neurons and neurons with round or stellate-shaped somas with small varicose dendrites. The morphology of calbindin D28 K-IR neurons was different from that of calretinin neurons. Anti-calbindin D28K-IR neurons usually had fusiform cell bodies and a thick primary dendrite with small branches forming a dendritic bouquet. Two-color immunofluorescence revealed that no cells expressed both proteins. Following unilateral enucleation a marked reduction of calretinin-IR fibers in the contralateral side to the enucleation was found. Enucleation appeared to have no effect on the cell bodies labeled with either protein. The results suggest the anti-calretinin immunoreactivity in the superficial layer of rabbit SC contrasts starkly with that of other animals.
authors.affiliation: Department of Biology, College of Natural Sciences, Kyungpook National University, Taegu, Korea.
authors.forename: C J
authors.initials: CJ
authors.lastname: Jeon
authors.forename: J K
authors.initials: JK
authors.lastname: Pyun
authors.forename: H W
authors.initials: HW
authors.lastname: Yang
mesh_terms: D000818:Animals; D064032:Calbindin 2; D064026:Calbindins; D015353:Eye Enucleation; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011817:Rabbits; D064030:S100 Calcium Binding Protein G / Q000032:analysis*; D013477:Superior Colliculi / Q000737:chemistry*
chemical_list: D064032:Calbindin 2; D064026:Calbindins; D009419:Nerve Tissue Proteins; D064030:S100 Calcium Binding Protein G