Determination of 13C/12C-ratios in rumen produced methane and CO2 of cows, sheep and camels.
Naturally produced methane shows different delta 13C-values with respect to its origin, e.g., geological or biological. Methane-production of ruminants is considered to be the dominant source from the animal kingdom. Isotopic values of rumen methane--given in literature--range between -80/1000 and -50/1000 and are related to feed composition and also sampling techniques. Keeping cows, camels and sheep under identical feed conditions and sampling rumen gases via implanted fistuale we compared delta PDB 13C-values of methane and CO2 between the species. Referring to mean values obtained from 4 or 5 samples at different times of 11 animals (n = 47) we calculated delta PDB 13C-medians resulting in small but not significant differences within and significant differences between the species for CO2 and methane. The delta PDB 13C-differences between methane and CO2 were statistically equal within and also between the species. Therefore a linear regression of methane values on CO2 is appropriate and leads to: delta PDB 13C(methane)/1000 = 1.57 * delta PDB 13C(CO2)/1000 - 47/1000 with a correlation coefficient of r = 0.87.
Schulze
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E
Fachgebiet Medizinische Physik der Tierärztlichen Hochschule Hannover, Deutschland.
Lohmeyer
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S
Giese
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W
D000818:Animals; D002162:Camelus; D002244:Carbon / Q000032:analysis; D002245:Carbon Dioxide / Q000737:chemistry / Q000378:metabolism*; D002247:Carbon Isotopes; D002417:Cattle; D005260:Female; D008297:Male; D008697:Methane / Q000737:chemistry / Q000378:metabolism*; D012417:Rumen / Q000378:metabolism*; D012756:Sheep; D013045:Species Specificity
D002247:Carbon Isotopes; D002245:Carbon Dioxide; D002244:Carbon; D008697:Methane