title: Gene expression of cyclooxygenase-2, group II and cytosolic phospholipase A2 in human colorectal cancer.
abstract: The development of intestinal tumours in the Min mouse is influenced by Moml locus, where the group II phospholipase A2 (PLA2-II) gene has been suggested as the candidate gene. Since no causative genetic alterations have been identified in PLA2-II in human colorectal tumours, we tested whether PLA2-II or the cytosolic isoform cPLA2, are dysregulated in human colorectal carcinogenesis. By means of RT-PCR, the expression of these genes and cyclooxygenase-2 (COX-2), which is upregulated in colorectal tumours, were examined. No changes in gene expression of PLA2-II were found in 44 tumours compared to normal mucosa. cPLA2 and COX-2 were upregulated; however, no correlation in expression was found between these genes. Thus, the supply of arachidonic acid provided by PLA2-II/cPLA2 seems not to be the rate limiting step in PGE2 (a prostaglandin/mitogen) formation via COX-2 and PLA2-II plays a minor or no role in human colorectal carcinogenesis.
authors.affiliation: Department of Biomedicine, Faculty of Health Sciences, Linköping, Sweden.
authors.forename: J
authors.initials: J
authors.lastname: Dimberg
authors.forename: A
authors.initials: A
authors.lastname: Samuelsson
authors.forename: A
authors.initials: A
authors.lastname: Hugander
authors.forename: P
authors.initials: P
authors.lastname: Söderkvist
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics; D051546:Cyclooxygenase 2; D003593:Cytoplasm / Q000201:enzymology; D005260:Female; D054501:Group II Phospholipases A2; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D010741:Phospholipases A / Q000235:genetics / Q000378:metabolism*; D054467:Phospholipases A2; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; D011451:Prostaglandin-Endoperoxide Synthases; D010741:Phospholipases A; D054501:Group II Phospholipases A2; D054467:Phospholipases A2