Centrifugal pulsing increases the recovery of viable spermatozoa.
OBJECTIVE
Our purpose was to compare the number and quality of spermatozoa recover following the standard centrifugation method with those after pulsing the relative centrifugal field during centrifugation.


METHODS
A prospectively controlled in vitro study using ejaculates from 10 healthy sperm donors was undertaken. Number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk results determined the efficacy of the centrifugation techniques.


RESULTS
The number of sperm and sperm motility at 0, 3, and 24 hr post-incubation at 5 degrees C in TEST-yolk were significantly higher for the sperm recovered following pulse centrifugation compared with those after standard centrifugation.


CONCLUSIONS
Pulsing the relative centrifugal field during centrifugation yields a higher number of viable spermatozoa.
Bielfeld
P
P
Department of Obstetrics and Gynecology, Heinrich Heine University, Dusseldorf, Germany.
Acosta
V
V
Jeyendran
R S
RS
D000328:Adult; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D008297:Male; D011446:Prospective Studies; D012661:Semen / Q000166:cytology*; D013081:Sperm Motility; D013094:Spermatozoa