title: Enhancement of XPG mRNA transcription by human interferon-beta in Cockayne syndrome cells with complementation group B.
abstract: Human interferon-beta (HuIFN-beta) confers UV-refractoriness in association with increased DNA repair capacity to human cells. We examined the modulation of XPG gene expression by HuIFN-beta in UV-sensitive cells from Cockayne syndrome complementation B (CSB), xeroderma pigmentosum complementation A (XPA) and normal control cells. Northern blot analysis revealed that XPG mRNA was more extensively transcribed in CSB cells treated with HuIFN-beta than in those without HuIFN-beta treatment. XPG mRNA from XPA cells and normal control cells was not markedly transcribed by HuIFN-beta treatment compared to that from CSB cells. The findings suggested that different mechanisms of UV-refractoriness by HuIFN-beta exist between CS and XP.
authors.affiliation: Department of Pediatrics, School of Medicine, Chiba University, Chibashi, Chiba 260-8677, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Sugita
authors.forename: N
authors.initials: N
authors.lastname: Suzuki
authors.forename: K
authors.initials: K
authors.lastname: Kita
authors.forename: Y
authors.initials: Y
authors.lastname: Higuchi
authors.forename: A
authors.initials: A
authors.lastname: Yamaura
authors.forename: Y
authors.initials: Y
authors.lastname: Kohno
mesh_terms: D015152:Blotting, Northern; D002461:Cell Line, Transformed; D003057:Cockayne Syndrome / Q000235:genetics* / Q000473:pathology; D004268:DNA-Binding Proteins / Q000235:genetics*; D004720:Endonucleases; D006801:Humans; D016899:Interferon-beta / Q000494:pharmacology*; D009687:Nuclear Proteins; D012313:RNA / Q000032:analysis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D014157:Transcription Factors; D014158:Transcription, Genetic / Q000187:drug effects; D000099250:DNA Excision Repair Protein ERCC-5
chemical_list: D004268:DNA-Binding Proteins; D004720:Endonucleases; D016899:Interferon-beta; D009687:Nuclear Proteins; D012313:RNA; D012333:RNA, Messenger; D014157:Transcription Factors; D000099250:DNA Excision Repair Protein ERCC-5