title: Immunohistochemistry of matrix metalloproteinases and their inhibitors in thoracic aortic aneurysms and aortic valves of patients with Marfan's syndrome.
abstract: BACKGROUND
  Thoracic aortic aneurysms (TAAs) and valvular insufficiency, the main cardiovascular lesions in Marfan's syndrome, are associated with destruction of connective tissue; however, their pathogenesis remains unclear.
  
  
  RESULTS
  To test the hypothesis that changes in the activity of the matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are responsible for the damage to connective tissue in these lesions, histochemical studies of the immunoreactivity (IR) for MMPs and their tissue TIMPs (MMP-1, MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2) were made in TAAs (n = 7) and aortic valves (n = 5) from 7 patients with Marfan's syndrome. All TAAs showed cystic medial necrosis (CMN), with loss of elastic fibers and smooth muscle cells. Extensive areas of myxoid change were found in all aortic valves. Areas of CMN showed no IR for any MMPs or TIMPs. The IR of smooth muscle cells at the borders of areas of CMN was stronger for all MMPs, especially MMP-2 and MMP-9, than in other regions. The surfaces of disrupted elastic fibers showed IR for MMP-2 and MMP-9. Areas of myxoid change showed similar but less pronounced alterations.
  
  
  CONCLUSIONS
  We hypothesize that the defect in fibrillin-1 in Marfan's syndrome leads to (1) formation of elastin that is abnormally aggregated and more easily degraded by MMPs than is normal elastin, (2) upregulation of the synthesis of MMPs, (3) progressive destruction of connective tissue by these enzymes, and (4) development of TAAs and valvular lesions.
authors.affiliation: St Luke's Episcopal Hospital, Houston, Tex., USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Segura
authors.forename: R E
authors.initials: RE
authors.lastname: Luna
authors.forename: K
authors.initials: K
authors.lastname: Horiba
authors.forename: W G
authors.initials: WG
authors.lastname: Stetler-Stevenson
authors.forename: H A
authors.initials: HA
authors.lastname: McAllister
authors.forename: J T
authors.initials: JT
authors.lastname: Willerson
authors.forename: V J
authors.initials: VJ
authors.lastname: Ferrans
mesh_terms: D000293:Adolescent; D000328:Adult; D001013:Aorta, Thoracic / Q000473:pathology; D017545:Aortic Aneurysm, Thoracic / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D001021:Aortic Valve / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D002648:Child; D005109:Extracellular Matrix / Q000201:enzymology*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008382:Marfan Syndrome / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D019278:Matrix Metalloproteinase 3; D008666:Metalloendopeptidases / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D008666:Metalloendopeptidases; D019278:Matrix Metalloproteinase 3