Rapid detection of Escherichia coli O157:H7 using immuno-magnetic flow cytometry in ground beef, apple juice, and milk.
A rapid method combining flow cytometry and immunomagnetic bead separation (IMFC) was effective for detecting low populations of Escherichia coli O157:H7 inoculated into ground beef, apple juice, and raw milk samples. Modified buffered peptone was most effective for enrichment of E. coli O157:H7 in ground beef. Using IMFC in combination with a 6-h enrichment, as few as four E. coli O157:H7 cells/g of ground beef were detected in as little as a 7-h total analysis time. Our results demonstrate the possibility of using flow cytometry and immunomagnetic bead separation to detect low concentrations of E. coli O157:H7 in ground beef, apple juice and milk.
Seo
K H
KH
Center for Food Safety and Quality Enhancement, Food Science & Technology, University of Georgia, Griffin 30223-1797, USA.
Brackett
R E
RE
Frank
J F
JF
D000818:Animals; D001628:Beverages / Q000382:microbiology*; D002417:Cattle; D004926:Escherichia coli / Q000302:isolation & purification*; D005434:Flow Cytometry; D006863:Hydrogen-Ion Concentration; D018189:Immunomagnetic Separation; D008460:Meat / Q000382:microbiology*; D008892:Milk / Q000382:microbiology*; D019685:Rosales