Does AK-2123 (Senazole) have sensitizing effects on radiation, cisplatin and hyperthermia under aerobic conditions in vitro?
We investigated the sensitizing effects of AK-2123 (Senazole) on the interaction of radiation, cisplatin and hyperthermia under aerobic conditions in the rat yolk sac cell line NMT-1R in vitro. The effects were assessed by clonogenic assay. A cytotoxic effect of AK-2123 after 24 hours exposure was observed as a function of the dose. For NMT-1R cells, the ID70 of AK-2123 was 400 micrograms/ml for 24 hours exposure, which was employed for subsequent combined treatments. Although a statistically significant increase in the G1 cell fraction was observed after AK-2123 treatment with a dose of ID70 (p = 0.02) no enhancing effect of AK-2123 on radiation, cisplatin or heat response curves was detected under aerobic conditions in vitro.
Mitsuhashi
N
N
Department of Radiology and Radiation Oncology, Gunma University School of Medicine, Japan. nmihashi@sb.gunma-u.ac.jp
Sakurai
H
H
Takahashi
T
T
Akimoto
T
T
Higuchi
K
K
Matsumoto
H
H
Ebara
T
T
Nozaki
M
M
Niibe
H
H
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects / Q000528:radiation effects; D002945:Cisplatin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D006979:Hyperthermia, Induced; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D051381:Rats; D014230:Triazoles / Q000494:pharmacology*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000528:radiation effects
D000970:Antineoplastic Agents; D011838:Radiation-Sensitizing Agents; D014230:Triazoles; C072690:AK 2123; D002945:Cisplatin