title: 5'-Peptidyl substituents allow a tuning of the affinity of oligodeoxyribonucleotides for RNA.
abstract: The affinity of amide-linked 5'-aminoacyl and 5'-dipeptidyl DNA octamers for two RNA undecamers with 3'-overhangs was measured via UV melting analysis. A sequence-dependent increase in melting points was observed. At low ionic strength, two appended lysine residues elevate melting points more than two additional A:U base pairs.
authors.affiliation: Department of Chemistry, Tufts University, Medford, MA 02155, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Sarracino
authors.forename: J A
authors.initials: JA
authors.lastname: Steinberg
authors.forename: M T
authors.initials: MT
authors.lastname: Vergo
authors.forename: G F
authors.initials: GF
authors.lastname: Woodworth
authors.forename: C N
authors.initials: CN
authors.lastname: Tetzlaff
authors.forename: C
authors.initials: C
authors.lastname: Richert
mesh_terms: D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism*; D009994:Osmolar Concentration; D012313:RNA / Q000737:chemistry / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004151:Dipeptides; D009838:Oligodeoxyribonucleotides; D012313:RNA; D004247:DNA