title: Substance P in proliferative vitreoretinopathy: the significance of aqueous humor levels for evolution of the disease.
abstract: OBJECTIVE
  We detected aqueous humor levels of substance P in patients with various grades of proliferative vitreoretinopathy and with uncomplicated rhegmatogenous retinal detachment. To evaluate the significance of the concentration of substance P at the time of surgery for retinal detachment for subsequent development of proliferative vitreoretinopathy, the latter patients also underwent fundoscopic control examination.
  
  
  METHODS
  Using a highly specific and sensitive radioimmunoassay, the content of substance P in fresh samples of aqueous humor obtained by paracentesis was determined both in cataract controls and in patients with uncomplicated rhegmatogenous retinal detachment and with various grades of proliferative vitreoretinopathy. Retinal detachment patients underwent fundoscopic control examination 6 months after surgical reattachment.
  
  
  RESULTS
  The mean concentration of substance P in cataract controls was 40.3 (+22.4) fmol/mg protein, in the retinal detachment group 61.9 (+/-13.9) fmol/mg protein and in proliferative vitreoretinopathy 335.2 (+/-24.8) fmol/mg protein, but no correlation between levels of the peptide and various grades of the disease was observed. Already at surgery for retinal detachment three in four patients who developed proliferative vitreoretinopathy presented with levels of substance P in the range of the disease.
  
  
  CONCLUSIONS
  The concentration of substance P in aqueous humor is significantly high in patients with proliferative vitreoretinopathy in whom surgery is indicated. Furthermore, elevation of the peptide in retinal detachment that originates most obviously from a neurogenic mechanism may indicate initiation of processes associated with proliferative vitreoretinopathy, thus representing an indicator of significant risk for evolution of the disease at a very early time.
authors.affiliation: Department of Ophthalmology and Optometry, University of Innsbruck, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Troger
authors.forename: B
authors.initials: B
authors.lastname: Kremser
authors.forename: E
authors.initials: E
authors.lastname: Irschick
authors.forename: W
authors.initials: W
authors.lastname: Göttinger
authors.forename: G
authors.initials: G
authors.lastname: Kieselbach
mesh_terms: D000328:Adult; D001082:Aqueous Humor / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism; D003452:Cryosurgery; D018450:Disease Progression; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D011446:Prospective Studies; D011863:Radioimmunoassay; D012163:Retinal Detachment / Q000378:metabolism / Q000601:surgery; D012591:Scleral Buckling; D013373:Substance P / Q000378:metabolism*; D014821:Vitrectomy; D018630:Vitreoretinopathy, Proliferative / Q000378:metabolism* / Q000601:surgery
chemical_list: D013373:Substance P