Fibre-fluorescence in situ hybridization unravels apparently seven DAZ genes or pseudogenes clustered within a Y-chromosome region frequently deleted in azoospermic males.
Using the technique of 'fibre-FISH' (fluorescence in situ hybridization), we describe the direct visualization of seven longer DAZ signal stretches and in addition a maximum of four isolated single DAZ signals on Y-chromatin fibres of four different individuals. These seven longer DAZ signal stretches may represent seven DAZ genes or pseudogenes, whereas the single DAZ signals may represent truncated DAZ genes.
Gläser
B
B
Institute of Human Genetics and Anthropology, University of Freiburg, Germany.
Yen
P H
PH
Schempp
W
W
D000075364:Deleted in Azoospermia 1 Protein; D006570:Heterochromatin / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D005810:Multigene Family; D009845:Oligospermia / Q000235:genetics*; D011544:Pseudogenes; D016601:RNA-Binding Proteins / Q000235:genetics*; D014998:Y Chromosome
C095668:DAZ1 protein, human; D000075364:Deleted in Azoospermia 1 Protein; D006570:Heterochromatin; D016601:RNA-Binding Proteins