Telomerase activity in human chorionic villi and placenta determined by TRAP and in situ TRAP assay.
Telomerase activity (TA) was analysed in human chorionic villi and placenta in normal and abnormal pregnancy using the telomeric repeat amplification protocol (TRAP) and in situ TRAP assay. Twenty chorionic villi specimens and 25 placenta specimens from normal pregnancies were examined as well as placenta specimens from 10 cases of intrauterine growth retardation (IUGR; nine asymmetric and one symmetric). TA was detected in 18 of the 20 (90 per cent) chorionic villi specimens and in 18 of the 25 (72 per cent) placenta specimens from normal pregnancy. However, no or only weak TA was exhibited in the placenta specimens of the nine asymmetric IUGR cases. In situ TRAP assay detected TA in trophoblastic cells from normal pregnancy, but not in trophoblastic cells from cases of asymmetric IUGR.
Izutsu
T
T
Department of Obstetrics and Gynecology, Iwate Medical University, Morioka, Japan.
Kudo
T
T
Sato
T
T
Nishiya
I
I
Ohyashiki
K
K
Mori
M
M
Nakagawara
K
K
D000328:Adult; D002824:Chorionic Villi / Q000201:enzymology*; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005317:Fetal Growth Retardation / Q000201:enzymology*; D006367:HeLa Cells; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D010920:Placenta / Q000201:enzymology; D016133:Polymerase Chain Reaction / Q000379:methods; D011247:Pregnancy / Q000378:metabolism*; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics
D017931:DNA Primers; D019098:Telomerase