Evaluation of resins for on-bead screening: a study of papain and chymotrypsin specificity using PEGA-bound combinatorial peptide libraries.
TentaGel, ArgoGel and PEGA resins were evaluated for on-bead biological screening, using a fluorescently-labelled peptide attached to each and assayed for papain activity. Peptide attached to PEGA was cleaved in near quantitative yield at the expected sites, whilst an identical sequence on TentaGel and ArgoGel beads was hydrolysed in very low yields and nonspecifically on ArgoGel. The compatibility of PEGA with enzymes was further demonstrated by the determination of subsite specificities of papain and chymotrypsin using PEGA-bound peptide libraries, which proved to be similar to those observed in free solution.
Leon
S
S
Dyson Perrins Laboratory, Oxford University, UK.
Quarrell
R
R
Lowe
G
G
D002918:Chymotrypsin / Q000378:metabolism*; D010206:Papain / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011092:Polyethylene Glycols; D012116:Resins, Plant
D010455:Peptides; D012116:Resins, Plant; C109511:bis(polyoxyethylene bis(amine)); D011092:Polyethylene Glycols; D002918:Chymotrypsin; D010206:Papain