Reduction of adhesion formation by intraperitoneal administration of Arg-Gly-Asp-containing peptides.
OBJECTIVE
To evaluate the ability of a variety of peptides containing the Arg-Gly-Asp (RGD) sequence to reduce the formation of intraperitoneal adhesions in a rabbit model.


METHODS
Prospective, randomized, double-blinded study.


METHODS
University-based laboratory.


METHODS
New Zealand white rabbits.


METHODS
Administration of RGD-containing peptides.


METHODS
Reduction of adhesion information.


RESULTS
In initial studies, two RGD-containing peptides (3 or a 10 amino acid peptides) were administered via Alzet miniosmotic pump to the site of injury. Administration of either of these peptides significantly reduced adhesion formation, but the larger peptide was more efficacious and reduced variability in the response. Further studies then were conducted with RGD-containing peptides five to six amino acids in length. Administration of these peptides also significantly reduced adhesion formation in a standard rabbit model. Administration of three of these peptides in a viscous vehicle at the end of surgery was also effective in reducing adhesion formation.


CONCLUSIONS
The most effective combination tested was RGD-containing peptide Gly-dser-Arg-Gly-Asp-Ser-Pro in a viscous, cremophor-containing vehicle. These studies demonstrate that administration of an RGD-containing peptide was effective in reducing adhesion formation in this model.
Rodgers
K E
KE
Department of Obstetrics and Gynecology, Livingston Research Center, University of Southern California, Los Angeles 90033, USA.
Girgis
W
W
Campeau
J
J
diZerega
G S
GS
D000818:Animals; D003131:Combined Modality Therapy; D004311:Double-Blind Method; D005260:Female; D015918:Infusion Pumps, Implantable; D009842:Oligopeptides / Q000627:therapeutic use*; D010529:Peritoneal Cavity; D011817:Rabbits; D000267:Tissue Adhesions / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*
D009842:Oligopeptides