MCP-1 and MIP-1alpha are most efficient in recruiting T cells into the skin in vivo.
To analyze T cell recruitment by chemokines in vivo, we used SCID mice grafted with human skin onto their backs and human T cells into their peritoneal cavities. rh MIP-1alpha and rh MCP-1, as well as mouse MCP-1, attracted significant amounts of human T cells into human skin grafts, whereas effects of rh RANTES, rh SDF-1, or rh IP-10 were minimal. Human T cells were found in a striking perivascular position exclusively at sites of human endothelium. None of the chemokines recruited human T cells across mouse endothelial barriers. For control purposes, chemokines were injected into the abdominal wall directly above the peritoneal cavity, where human T cells had been injected and were not subjected to flow. At this site all tested chemokines attracted human T cells to an equal extent. In summary, our experiments point towards an important role for MCP-1 and MIP-1alpha as T cell chemoattractants for skin inflammatory conditions.
Kunstfeld
R
R
Department of Dermatology, University of Vienna Medical School, Austria.
Lechleitner
S
S
Wolff
K
K
Petzelbauer
P
P
D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D018932:Chemokine CCL2 / Q000494:pharmacology*; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines / Q000502:physiology; D004730:Endothelium, Vascular / Q000166:cytology; D006801:Humans; D019402:Macrophage Inflammatory Proteins / Q000494:pharmacology*; D051379:Mice; D016513:Mice, SCID; D010641:Phenotype; D016038:Skin Transplantation / Q000276:immunology* / Q000473:pathology*; D013601:T-Lymphocytes / Q000166:cytology*; D014183:Transplantation, Heterologous
D018932:Chemokine CCL2; D054405:Chemokine CCL3; D054407:Chemokine CCL4; D018925:Chemokines; D019402:Macrophage Inflammatory Proteins