Stable cationic capillary coating with successive multiple ionic polymer layers for capillary electrophoresis.
A coated capillary modified with a cationic polymer was developed by using a novel coating procedure, successive multiple ionic-polymer (SMIL) coating. The SMIL coating was achieved by first attaching the cationic polymer to the capillary inner wall, and then the anionic polymer to the cationic polymer layer, and finally the cationic polymer to the anionic polymer layer. The stability of Polybrene (PB)-modified capillary made by SMIL coating was remarkably improved in comparison with a conventional PB-modified capillary. It endured during 600 replicate analyses and also showed strong stability against 1 M NaOH and 0.1 M HCl. The relative standard deviation of the run-to-run, day-to-day, and capillary-to-capillary coating was all below 1%, and good reproducibilities were obtained. The PB-modified capillary made by SMIL coating was applied to the basic protein analyses. It gave good performances for the protein analyses even when the pH of the electrolyte was near the isoelectric point (pI) of the protein. In addition, 0.1 M NaOH rinse prior to the sample injection allowed the reproducible analysis of a highly adsorptive sample such as plasma because the adsorbed sample could be flushed out of the capillary. Besides protein analyses, an efficient analysis of the cationic drugs by capillary electrophoresis/mass spectrometry (CE/MS) was also possible.
Katayama
H
H
Department of Analytical Chemistry, Analytical Research Laboratories, Eisai Co., Ltd., Ibaraki, Japan. h1-katayama@eisai.co.jp
Ishihama
Y
Y
Asakawa
N
N
D002412:Cations; D019075:Electrophoresis, Capillary / Q000379:methods*; D006583:Hexadimethrine Bromide; D011506:Proteins / Q000032:analysis; D015203:Reproducibility of Results
D002412:Cations; D011506:Proteins; D006583:Hexadimethrine Bromide