[Chromatography of thrombin and antithrombin III on biospecific silochromes].
It was shown that the seaweed sulfopolysaccharides Furcellaria and Phyllophora--furcellaran and phyllophoran--are the competitive inhibitors of thrombin-induced fibrinogen clotting. Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Possible application of the synthesized adsorbents both for thrombin, AT III purification from blood products and for alpha-thrombin, beta and gamma-thrombin separation are discussed.
Lohins'ka
Z V
ZV
Lviv Branch of Kyiev Research Institute of Haematology and Blood Transfusion.
Oryshchenko
N V
NV
Vus
M M
MM
Maherovskyĭ
Iu V
IuV
Haĭda
H Z
HZ
Haĭda
A V
AV
D000464:Alginates / Q000737:chemistry*; D000990:Antithrombin III / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D053149:Plant Gums; D058428:Silica Gel; D012822:Silicon Dioxide / Q000737:chemistry*; D013917:Thrombin / Q000032:analysis* / Q000037:antagonists & inhibitors
D000464:Alginates; D053149:Plant Gums; D058428:Silica Gel; D012822:Silicon Dioxide; C012985:furcellaran; D000990:Antithrombin III; D013917:Thrombin
Khromatohrafiia trombinu ta antytrombinu III na biospetsyfichnykh sylokhromakh.