title: Actin gene sequence from Euglena gracilis.
abstract: The full length coding sequence of the Euglena gracilis actin gene was determined by RT-PCR of Euglena gracilis mRNA. Conserved regions in the actin amino acid sequence were used as guides for the synthesis of degenerate primers. Sequence was obtained for 1,238 nucleotides, of which 1,131 were coding for 377 amino acids. Sequence comparisons showed a similarity with other actins of 56% to 80%. Even though most of the actin amino acid sequence was conserved, some regions showed high divergence, i.e. the DNase I-binding loop at the N-terminal region. The construction of a phylogenetic tree based on actin sequences from different organisms placed Euglena gracilis in a cluster with Trypanosoma brucei and Leishmania major.
authors.affiliation: Department of Plant Biology, Lund University, Sweden.
authors.forename: S K
authors.initials: SK
authors.lastname: Petersen-Mahrt
authors.forename: S K
authors.initials: SK
authors.lastname: Petersen-Mahrt
authors.forename: S
authors.initials: S
authors.lastname: Widell
mesh_terms: D000199:Actins / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016054:DNA, Protozoan; D005056:Euglena gracilis / Q000235:genetics*; D017125:Genes, Protozoan; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid
chemical_list: D000199:Actins; D016054:DNA, Protozoan