Association of N-acetyllactosamine with tumor progression in human breast cancer: a study using a 16 kDa chick embryo lectin.
This study documents modifications in the expression and the cellular distribution of binding sites for a 16 kDa chick embryo lectin (CL16-BS) in breast cancer. This lectin binds preferentially to terminal and penultimate N-acetyllactosamine residues (Galbeta1-4GlcNAc). BS density and distribution, studied by lectin binding followed by indirect immunofluorescence, were compared in normal breast tissues and 45 invasive carcinomas (lobular and ductal). Increased number of fluorescent epithelial cells (ETC+) were observed in normal ducts adjacent to lobular carcinomas and in tumors from both types when compared to normal glands. In ductal carcinomas, a significant diminution of ETC+ percentage was observed in the highest anatomopathological SBR grades: 32.7% in grade III, 80.8% in grade II and 66.5% in grade I (p<0.001). For both lobular and ductal carcinomas, ETC+ percentages were also positively correlated with low versus high MSBR grades (p<0.002). The subcellular distribution of CL16-BS varied according to the tumor type and/or the histological grades. It was mostly membrane-associated in low SBR and MSBR grades (p<0.001 and p<0.01, respectively) and cytoplasm-associated in high grades (p<0.02 and p<0.05). Some of these parameters were also correlated with certain other clinicopathological factors, such as tumor size (p<0.02), high S-phase cell fraction (p<0.04 and p<0.03) and low density estrogen receptors (p<0.05). Diminution in CL16-BS density and cytoplasmic versus membrane localization may be considered as indicators of tumor progression but not of metastasis.
Henou
C
C
Laboratoire d'Immunologie, Université de Clermont Blaise Pascal, 63177Aubière Cedex, France.
Didier
E
E
De Latour
M
M
Kwiatkowski
F
F
Communal
Y
Y
Besse
G
G
Bignon
Y
Y
D000606:Amino Sugars / Q000032:analysis*; D000818:Animals; D001940:Breast / Q000737:chemistry / Q000166:cytology; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology*; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000473:pathology*; D018275:Carcinoma, Lobular / Q000737:chemistry / Q000473:pathology*; D002642:Chick Embryo; D005260:Female; D018226:Fibroadenoma / Q000737:chemistry / Q000473:pathology; D006801:Humans; D037102:Lectins / Q000032:analysis*; D009361:Neoplasm Invasiveness; D012016:Reference Values
D000606:Amino Sugars; D037102:Lectins; C000458:N-acetyllactosamine