[Evidence of a renin receptor on human mesangial cells: effects on PAI1 and cGMP].
Most proteases a receptor or a binding site that serves to concentrate the proteolytic activity on the cell surface and to mediate cellular effects. We looked for such a receptor for renin, an aspartyl protease. The binding of recombinant human renin labelled with 125I was studied on primary and immortalized human mesangial cells. The binding of renin was specific, saturable and was characterized by Kd = 0.4 nM and 8,000 sites/cell and Kd = 1 nM and 2,000 sites/cell for primary and immortalized cells, respectively. The binding did not depend on the active site of the enzyme, was not followed by internalization and degradation of renin and did not modify intracellular Ca2+. Stimulation of primary cells with 100 nM induced a significant increase of 3H thymidine incorporation but was not associated with an increase of the cell number. Furthermore, incubation of mesangial cells 24 h with 100 nM renin provoked an increase of tPA and of PAI1 in the conditioned medium. This increase was not modified neither by captopril nor by angiotensin II receptors antagonists. The tPA antigen elevation was confirmed by fibrin zymography showing an increase of tPA/PAI1 complexes. But, surprisingly, the reverse zymogram showed that PAI antigen increase was associated with decreased PAI activity which was due to PAI clivage in an inactive form. PAI clivage by renin required the presence of the cells and could not be obtained by incubating renin and recombinant human PAI alone. When primary mesangial cells were cultured in the presence of a specific inhibitor of renin active site, RO 42-5982, PAI accumulation in the conditioned medium was reduced by 50-60%, suggesting that endogenous renin plays a role in PAI synthesis and/or secretion. The binding of renin does not induce cAMP and cGMP generation. However, in the presence of renin (100 nM and 1 microM) the extent of cGMP generated by CNP (10 and 100 nM) was reduced by 50%. Preliminary results of the renin receptor purification by affinity chromatography indicate that the receptor Mr is about 57 kDa.
Nguyen
G
G
INSERM U 489, Hôpital Tenon, Paris.
Bouzhir
L
L
Delarue
F
F
Rondeau
E
E
Sraer
J D
JD
D002118:Calcium / Q000378:metabolism; D006152:Cyclic GMP / Q000096:biosynthesis*; D005920:Glomerular Mesangium / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D007457:Iodine Radioisotopes; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000032:analysis* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D012083:Renin / Q000378:metabolism* / Q000494:pharmacology; D010959:Tissue Plasminogen Activator / Q000378:metabolism
D007457:Iodine Radioisotopes; D017395:Plasminogen Activator Inhibitor 1; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D010959:Tissue Plasminogen Activator; D012083:Renin; D006152:Cyclic GMP; D002118:Calcium
Mise en évidence d'un récepteur de la rénine sur les cellules mésangiales humaines: effets sur le PAI1 et sur le GMPc.