Sequence of a pyrimidine-selective Na+/nucleoside cotransporter from pig kidney, pkCNT1.
A 3 kb cDNA called pkCNT1, a new member of the Na+/nucleoside cotransporter family, was cloned from pig kidney and sequenced. The sequence of pkCNT1 encodes a 647 amino acid protein that is 84% identical to the sequence of the rat pyrimidine-selective Na+/nucleoside cotransporter, rCNT1. pkCNT1 transports pyrimidines, such as thymidine and uridine, and has a Km for uridine of 9 microM.
Pajor
A M
AM
University of Arizona, Department of Physiology, College of Medicine, Tucson, AZ 85724-5051, USA.ampajor@utmb.edu
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D007668:Kidney / Q000737:chemistry*; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D011684:Purine Nucleosides / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013552:Swine
D002352:Carrier Proteins; D017931:DNA Primers; D026901:Membrane Transport Proteins; D011684:Purine Nucleosides; D011743:Pyrimidines; C065171:cif nucleoside transporter