Interaction between sodium 5,6-benzylidene-L-ascorbate and dopamine.
The interaction between sodium 5,6-benzylidene-L-ascorbate (SBA) and dopamine was investigated by three different parameters: radical intensity, prooxidant action and cytotoxic activity. Under alkaline conditions, SBA and dopamine produced doublet and quartet ESR signals, respectively. The addition of increasing concentrations of SBA completely scavenged the dopamine radical and replaced the latter with its own radical. On the other hand, dopamine accelerated the decay of SBA radical. These two compounds stimulated the methionine oxidation in culture medium, but in combination, their stimulation activities were weakened. Both of these two compounds dose-dependently reduced the viable cell number of human promyelocytic leukemics HL-60 cells. When these two compounds were mixed together before adding to HL-60 cells, both of their cytotoxic activities were diminished. The present study demonstrates the interaction between SBA and dopamine, which might modify their biological activities.
Satoh
K
K
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Ida
Y
Y
Kochi
M
M
Sakagami
H
H
D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D001597:Benzylidene Compounds / Q000737:chemistry / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D004298:Dopamine / Q000737:chemistry / Q000494:pharmacology*; D004347:Drug Interactions; D005609:Free Radicals; D018922:HL-60 Cells / Q000187:drug effects; D006801:Humans; D008715:Methionine / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction
D000970:Antineoplastic Agents; D000975:Antioxidants; D001597:Benzylidene Compounds; D005609:Free Radicals; D008715:Methionine; D001205:Ascorbic Acid; C070680:zilascorb; D004298:Dopamine