Detection of Cryptosporidium parvum in horses: thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry.
Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.
Cole
D J
DJ
Department of Large Animal Medicine and Surgery, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4475, USA. dcole@sph.unc.edu
Snowden
K
K
Cohen
N D
ND
Smith
R
R
D000818:Animals; D003457:Cryptosporidiosis / Q000469:parasitology / Q000662:veterinary*; D016785:Cryptosporidium parvum / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006734:Horse Diseases / Q000175:diagnosis* / Q000469:parasitology; D006736:Horses; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods