title: Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein.
abstract: A recombinant human antibody fragment, designated RSV Fab 19, efficiently neutralizes respiratory syncytial virus (RSV). Here we report the results of our sequence analysis of antibody escape mutants that identified F glycoprotein amino acids critical for binding of human or murine RSV F-neutralizing antibodies.
authors.affiliation: Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892-0720, USA. james.e.crowe@vanderbilt.edu
authors.forename: J E
authors.initials: JE
authors.lastname: Crowe
authors.forename: C Y
authors.initials: CY
authors.lastname: Firestone
authors.forename: R
authors.initials: R
authors.lastname: Crim
authors.forename: J A
authors.initials: JA
authors.lastname: Beeler
authors.forename: K L
authors.initials: KL
authors.lastname: Coelingh
authors.forename: C F
authors.initials: CF
authors.lastname: Barbas
authors.forename: D R
authors.initials: DR
authors.lastname: Burton
authors.forename: R M
authors.initials: RM
authors.lastname: Chanock
authors.forename: B R
authors.initials: BR
authors.lastname: Murphy
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D009500:Neutralization Tests; D017354:Point Mutation; D011994:Recombinant Proteins; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014760:Viral Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins