Characterisation of the murine gene encoding the intracellular hyaluronan receptor IHABP (RHAMM).
We have recently shown that the published cDNA sequence encoding the murine cell surface receptor for hyaluronan-mediated motility (RHAMM) in fact represents a partial sequence of the cDNA encoding a new intracellular hyaluronic acid binding protein (IHABP). Here we publish the genomic organisation, including 700bp sequences of the promoter region, of the IHABP gene. The IHABP gene consists of 18 exons and spans more than 25kb. Part of the IHABP gene is identical with the published data on RHAMM. The IHABP gene apparently possesses one promoter region with one major transcriptional start point. IHABP is ubiquitously expressed at the mRNA and the protein level in all murine tissues, suggesting that the function of this intracellular hyaluronan binding protein is not restricted to migrating cells.
Fieber
C
C
Forschungszentrum Karlsruhe, Institute of Genetics, D-76021, Karlsruhe, Germany.
Plug
R
R
Sleeman
J
J
Dall
P
P
Ponta
H
H
Hofmann
M
M
D020121:5' Untranslated Regions; D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D005091:Exons; D016326:Extracellular Matrix Proteins / Q000235:genetics* / Q000378:metabolism*; D005786:Gene Expression Regulation; D018960:Hyaluronan Receptors / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D016329:Sp1 Transcription Factor / Q000378:metabolism; D014018:Tissue Distribution; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014158:Transcription, Genetic
D020121:5' Untranslated Regions; D016326:Extracellular Matrix Proteins; D018960:Hyaluronan Receptors; D016329:Sp1 Transcription Factor; D018808:Transcription Factor AP-1; C098946:hyaluronan-mediated motility receptor