Matrix metalloproteinase-1 in cholesteatoma, middle ear granulations and deep meatal skin: a comparative analysis.
Nine cholesteatomas, seven middle ear granulations and five deep meatal skin specimens were analysed for gelatinase activity at molecular weights corresponding to those of matrix metalloproteinase-1 (MMP-1) using SDS PAGE zymography. Gelatinase activity at 41-43 kDa and 45-47 kDa was investigated. Western blotting was employed using a primary monoclonal antibody to MMP-1 to provide a qualitative assessment of MMP-1. Western blotting was also used with a monoclonal antibody to MMP-3 to discover if MMP-3 gelatinase activity occurring around the molecular weight of MMP-1 may have contributed to the results. A significantly higher expression of activity was recorded in cholesteatoma and middle ear granulations at 45-47 kDa in comparison with deep meatal skin. Western blotting indices were to be present in all of the cholesteatoma specimens tested. Only one of the specimens (cholesteatoma) tested showed any MMP-3 presence.
Banerjee
A R
AR
Department of ENT, Leicester Royal Infirmary, UK.
James
R
R
Narula
A A
AA
Lee
R J
RJ
D015153:Blotting, Western / Q000379:methods; D018424:Cholesteatoma, Middle Ear / Q000201:enzymology*; D017364:Collagenases / Q000032:analysis*; D004432:Ear, Middle / Q000737:chemistry* / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006097:Granulation Tissue / Q000737:chemistry* / Q000201:enzymology; D006801:Humans; D020781:Matrix Metalloproteinase 1; D019278:Matrix Metalloproteinase 3 / Q000032:analysis; D008970:Molecular Weight; D012867:Skin / Q000737:chemistry* / Q000201:enzymology
D017364:Collagenases; D019278:Matrix Metalloproteinase 3; D020781:Matrix Metalloproteinase 1