Intracellular detection assays for high-throughput screening.
New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-engineered biosensors for intracellular biochemistry, specifically localized targets, and protein-protein interactions.
González
J E
JE
Aurora Biosciences Corp 11010 Torreyana Road San Diego CA 92121 USA.
Negulescu
P A
PA
D000818:Animals; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004798:Enzymes / Q000378:metabolism; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D006801:Humans; D007473:Ion Channels / Q000502:physiology; D008163:Luminescent Measurements; D008164:Luminescent Proteins / Q000032:analysis / Q000235:genetics; D011956:Receptors, Cell Surface / Q000502:physiology; D011994:Recombinant Proteins / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000379:methods; D014162:Transfection
D004798:Enzymes; D007473:Ion Channels; D008164:Luminescent Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins