title: Nucleotide sequences of the 26S mRNAs of the viruses defining the Venezuelan equine encephalitis antigenic complex.
abstract: Genetic relationships among viruses defining the Venezuelan equine encephalitis (VEE) virus antigenic complex were determined by analyzing the 3'-terminal 561 nucleotides of the nonstructural protein 4 gene and the entire 26S RNA region of the genome. New sequence information is reported for VEE 78V-3531 (VEE subtype-variety IF), Mucambo (IIIA), Tonate (IIIB), 71D-1252 (IIIC), Pixuna (IV), Cabassou (V), and AG80-663 (VI) viruses. The results reported here and by previous investigators largely support the current classification scheme of these viruses, while clearly identifying Everglades (II) as a subtype I virus. A genetic relationship between 78V-3531 (IF) and AG80-663 (VI) viruses contradicted previous serologic results. Mutations near the amino terminus of the E2 envelope proteins of Pixuna and AG80-663 viruses probably account for the previously reported low reactivity of the protective monoclonal antibody 1A2B-10 with these two viruses. Variations in the distribution of potential glycosylation sites in the E2 glycoprotein are discussed.
authors.affiliation: Arbovirus Diseases Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Kinney
authors.forename: M
authors.initials: M
authors.lastname: Pfeffer
authors.forename: K R
authors.initials: KR
authors.lastname: Tsuchiya
authors.forename: G J
authors.initials: GJ
authors.lastname: Chang
authors.forename: J T
authors.initials: JT
authors.lastname: Roehrig
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry; D004666:Encephalitis Virus, Venezuelan Equine / Q000145:classification / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000737:chemistry*; D012367:RNA, Viral / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D000956:Antigens, Viral; D018076:DNA, Complementary; D012333:RNA, Messenger; D012367:RNA, Viral; D014759:Viral Envelope Proteins