Analysis of the human RAD51L1 promoter region and its activation by UV light.
The human REC2/RAD51B gene (HGMW-approved symbol RAD51L1) encodes a 350-amino-acid protein with regional homologies to members of the RAD52 epistasis group. It is induced by DNA-damaging agents, and the overexpression of this gene product causes G1/S cell cycle arrest. In this report, the promoter region, containing the UV-responsive element, is revealed. Deletion analyses of a 1699-base fragment at the 5' end of the human REC2/RAD51B cDNA identified a 116-base sequence that appears to be responsible for radiation induction. This fragment contains many DNA sequences that have been identified in the promoter regions of other radiation-inducible genes in yeast and humans. Within this region are "consensus" binding sites for both the AP2 and the p53 proteins that may act to regulate the expression of the human REC2/RAD51B gene. Five putative transcripts have been identified from regions 5' of the promoter element that splice near the ATG translation start site. None of the transcripts contain the UV-inducible element nor the consensus transcription factor binding sites.
Peng
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Kimmel Cancer Center/Jefferson Center for Biomedical Research, Thomas Jefferson University, 700 East Butler Avenue, Doylestown, Pennsylvania, 18901-2697, USA.
Rice
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Kmiec
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D001483:Base Sequence; D003001:Cloning, Molecular; D004249:DNA Damage / Q000235:genetics / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000528:radiation effects*; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000528:radiation effects*; D011506:Proteins / Q000235:genetics* / Q000378:metabolism / Q000528:radiation effects*; D051135:Rad51 Recombinase; D014158:Transcription, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D004268:DNA-Binding Proteins; D011506:Proteins; C413002:RAD51B protein, human; D016159:Tumor Suppressor Protein p53; C495153:RAD51 protein, human; D051135:Rad51 Recombinase