Antimicrobial activity and conformation of gaegurin-6 amide and its analogs.
A function of the intra-disulfide bridge located at the C-terminal of Rana peptides has not been extensively studied. To investigate the function of the disulfide bridge related to the activity and the structure, we chose Gaegurin-6, isolated from Rana rugosa as a model peptide and synthesized linear analogs. The reduction of the disulfide bridge resulted in the complete loss of antimicrobial activity while replacements of cysteines by serines retained antimicrobial activity. Circular dichroism spectra from a titration of the peptides in sodium dodecyl sulfate indicated that the disulfide bridge of Gaegurin-6 might stabilize the induction of an alpha helical structure in lipid membranes and the alpha helical forming propensity of the peptides correlated with antimicrobial activity.
Lee
K H
KH
Protein Chemistry Laboratory, Mogam Biotechnology Research Institute, Kyunggi-Do, South Korea. lkh@kgcc.co.kr
Hong
S Y
SY
Oh
J E
JE
Lee
B J
BJ
Choi
B S
BS
D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002942:Circular Dichroism; D004220:Disulfides / Q000494:pharmacology; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D011498:Protein Precursors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017433:Protein Structure, Secondary / Q000187:drug effects; D011898:Ranidae; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000890:Anti-Infective Agents; D004220:Disulfides; D010455:Peptides; D011498:Protein Precursors; C119329:gaegurin 6; D012967:Sodium Dodecyl Sulfate