title: Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo.
abstract: We established a xenograft line of human adrenocortical carcinoma (ADR-1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320+/-1420 pg/ml; control 191+/-130 pg/ml) and hypokalemia (serum K 3.4+/-0.4 mEq/l; control 5.2+/-1.0 mEq/l) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.
authors.affiliation: Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Y
authors.initials: Y
authors.lastname: Abe
authors.forename: Y
authors.initials: Y
authors.lastname: Katoh
authors.forename: N
authors.initials: N
authors.lastname: Sawa
authors.forename: Y
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: H
authors.initials: H
authors.lastname: Sasano
authors.forename: Y
authors.initials: Y
authors.lastname: Oshika
authors.forename: T
authors.initials: T
authors.lastname: Tokunaga
authors.forename: H
authors.initials: H
authors.lastname: Kijima
authors.forename: N
authors.initials: N
authors.lastname: Tamaoki
authors.forename: M
authors.initials: M
authors.lastname: Nakamura
authors.forename: Y
authors.initials: Y
authors.lastname: Ueyama
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000473:pathology / Q000503:physiopathology*; D000328:Adult; D000818:Animals; D001794:Blood Pressure; D002277:Carcinoma / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D014183:Transplantation, Heterologous