Distribution of somatostatin receptor subtypes in rat lumbar spinal cord examined with gold-labelled somatostatin and anti-receptor antibodies.
Using gold-labelled somatostatin, somatostatin binding sites were predominantly found in laminae I-III, X and on motorneurones of the rat lumbar spinal cord. A comparison with immunohistochemical staining using antisera against somatostatin receptor sequences revealed that the marked binding in laminae I-III coincided with the presence of somatostatin receptor-like immunoreactivity for the receptor subtypes 1, 2 and 3. Binding sites on motorneurones were only paralleled by an immunoreaction for subtype 3. In lamina X, however, the lack of a positive immunoreaction indicates that in this part other subtypes may be present.
Segond von Banchet
G
G
Physiologisches Institut I, Universität Jena, Teichgraben 8, D-07743, Jena, Germany.
Schindler
M
M
Hervieu
G J
GJ
Beckmann
B
B
Emson
P C
PC
Heppelmann
B
B
D000818:Animals; D007150:Immunohistochemistry; D008161:Lumbosacral Region; D008297:Male; D009046:Motor Neurons / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D017481:Receptors, Somatostatin / Q000276:immunology / Q000378:metabolism*; D013004:Somatostatin / Q000378:metabolism*; D013116:Spinal Cord / Q000378:metabolism*
D017481:Receptors, Somatostatin; D013004:Somatostatin