title: Volatile anaesthetics induce changes in the expression of P-selectin and glycoprotein Ib on the surface of platelets in vitro.
abstract: Halothane has been shown to inhibit platelet aggregation and may, therefore, prevent intraoperative platelet activation. The aim of this study was to compare the effects of volatile anaesthetics on platelet activation, including the transformation into an adhesive platelet phenotype. After in vitro exposure to volatile anaesthetics, the expression of the adhesion molecule P-selectin and the internalization of the receptor for the von Willebrand factor (GPlb) were analysed by flow cytometry. In contrast to desflurane or N2O, sevoflurane (> or = 0.5 MAC, P < 0.05), halothane (> or = 1.0 MAC, P < 0.01) and isoflurane (> or = 2.0 MAC, P < 0.01) induced a significantly higher expression of P-selectin on the surface of platelets, indicating the degranulation of alpha-granules. In the presence of desflurane (> or = 0.5 MAC, P < 0.05), halothane (> or = 1.0 MAC, P < 0.01), isoflurane and sevoflurane (both > or = 2.0 MAC, P < 0.01), a redistribution of GPlb occurred, indicating platelet activation. N2O had no effect. In conclusion, several of the volatile anaesthetics tested in vitro induced changes in the expression of P-selectin and GPlb, being characteristic of platelet activation. None of the anaesthetics investigated interfered with the platelet response to ADP stimulation.
authors.affiliation: Department of Anaesthesia, University of Regensburg, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Fröhlich
authors.forename: G
authors.initials: G
authors.lastname: Rothe
authors.forename: G
authors.initials: G
authors.lastname: Schmitz
authors.forename: E
authors.initials: E
authors.lastname: Hansen
mesh_terms: D000328:Adult; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D001792:Blood Platelets / Q000378:metabolism*; D000077335:Desflurane; D005434:Flow Cytometry; D008401:Gas Chromatography-Mass Spectrometry; D006221:Halothane / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007530:Isoflurane / Q000031:analogs & derivatives / Q000494:pharmacology; D008738:Methyl Ethers / Q000494:pharmacology; D009609:Nitrous Oxide / Q000494:pharmacology; D019007:P-Selectin / Q000097:blood*; D015539:Platelet Activation / Q000187:drug effects; D019038:Platelet Glycoprotein GPIb-IX Complex / Q000378:metabolism*; D000077149:Sevoflurane
chemical_list: D018685:Anesthetics, Inhalation; D008738:Methyl Ethers; D019007:P-Selectin; D019038:Platelet Glycoprotein GPIb-IX Complex; D000077149:Sevoflurane; D000077335:Desflurane; D007530:Isoflurane; D009609:Nitrous Oxide; D006221:Halothane