title: Identification of particular epithelial areas and cells that transport polypeptide-coated nanoparticles in the nasal respiratory mucosa of the rabbit.
abstract: The active transcytosis of many different polypeptides (either presented free or adsorbed on latex nanoparticles), found in the respiratory mucosa of the upper nasal concha, has previously been shown to be proportional to the total volume of the lymphoid aggregates present in the tissue. By combining the use of fluorescent nanoparticles, flux measurements, confocal and scanning electron microscopy and conventional histology, it is shown in this paper that: (i) the areas of epithelium overlying lymphoid aggregates are the only transporting polypeptides; (ii) the respiratory epithelium in these areas consists mainly of non-ciliated microvillar cells, with numerous ciliated cells and rare mucous goblet cells at the periphery of the area only; (iii) non-ciliated microvillar cells are distinguishable in cells with well developed finger-like microvilli and cells with an irregularly pleated apical membrane, similar to that of intestinal and bronchial antigen-sampling M-cells; (iv) groups of polypeptide-coated nanospheres are found bound to this latter type of cells, demonstrating that these are the transporting cells, detected at the first stage of the transcytotic cycle.
authors.affiliation: Dipartimento di Fisiologia e Biochimica Generali (Sezione di Fisiologia Generale), Università degli Studi di Milano, via Celoria 26, I-20133 Milan, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Ghirardelli
authors.forename: F
authors.initials: F
authors.lastname: Bonasoro
authors.forename: C
authors.initials: C
authors.lastname: Porta
authors.forename: D
authors.initials: D
authors.lastname: Cremaschi
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D004848:Epithelium / Q000378:metabolism*; D005453:Fluorescence; D066298:In Vitro Techniques; D008297:Male; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008871:Microvilli / Q000378:metabolism; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D010455:Peptides / Q000378:metabolism*; D011817:Rabbits
chemical_list: D010455:Peptides