title: Fluorophotometric assessment of rabbit corneal epithelial barrier function after rigid contact lens wear.
abstract: OBJECTIVE
  The effect of overnight wear of graded rigid gas-permeable (RGP) contact lenses on the rabbit corneal epithelial barrier function was assessed by fluorophotometry.
  
  
  METHODS
  RGP lenses with optimal fitting were worn by rabbits for 24 h. The values of oxygen transmissibility (Dk/L) of lenses used were 0, 10, 27, 43, 84, and 109 x 10(-9) (cm/s)(ml O2/ml mm Hg); lens thickness (0.15 mm) and diameter (14.0 mm) were standardized. Corneal epithelial barrier function was measured by a slit-lamp fluorophotometer. Corneal surface lesions by fluorescein staining were observed by a slit-lamp biomicroscope with the aid of fluorescein filter paper and were scored. Corneal epithelial structure was observed by scanning electron microscopy (SEM).
  
  
  RESULTS
  After 24-h wear of RGP lenses, fluorescein uptake by the cornea increased, and this increase depended on the Dk/L value of lenses worn; mean +/- standard error, 1,601+/-454 (Dk/L 0), 347+/-226 (Dk/L 10), 144+/-42 (Dk/L 27), 100+/-25 (Dk/L 43), 56+/-18 (Dk/L 84), 51+/-15 (Dk/L 109), and 49+/-4 ng/ml (control). There was a significant correlation between the fluorophotometric value and the biomicroscopic scores of corneal lesions. SEM showed progressive desquamation of epithelial superficial and wing cells for lower-value Dk/L lens groups.
  
  
  CONCLUSIONS
  This study demonstrated for the first time that overnight wear of Dk/L 84 and 109 lenses does not alter the barrier function of the rabbit corneal epithelium. In contrast, wear of a PMMA lens greatly alters the corneal epithelial barrier function. It is suggested that RGP lenses with a Dk/L >80 can provide safe overnight wear, based on the epithelial barrier function.
authors.affiliation: R&D Department, Menicon Co., Ltd., Aichi, Kasugai, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Ichijima
authors.forename: N
authors.initials: N
authors.lastname: Yokoi
authors.forename: A
authors.initials: A
authors.lastname: Nishizawa
authors.forename: S
authors.initials: S
authors.lastname: Kinoshita
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability; D003261:Contact Lenses; D019573:Epithelium, Corneal / Q000502:physiology* / Q000648:ultrastructure; D019793:Fluorescein / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D015590:Fluorophotometry; D005500:Follow-Up Studies; D008855:Microscopy, Electron, Scanning; D010100:Oxygen / Q000378:metabolism; D019904:Polymethyl Methacrylate; D011817:Rabbits
chemical_list: D005456:Fluorescent Dyes; D019904:Polymethyl Methacrylate; D010100:Oxygen; D019793:Fluorescein