GC/MS analysis of m-hydroxybenzoylecgonine in urine. Forensic implication in cocaine use.
m-Hydroxybenzoylecgonine (m-OH-BE) and d3-m- hydroxybenzoylecgonine (d3-m-OH-BE) have been synthesized, and a GC/MS procedure with d3-m-OH-BE as internal standard has been developed. Among 24 human urine specimens that were positive for BE, all of them have shown detectable levels of m-OH-BE with 75% of the specimens exceeding the LoQ (5 ng/mL), compared with only 50% of the specimens containing detectable levels of EME. The presence of m-OH-BE in urine suggested that this metabolite may serve as a valuable marker of cocaine use in addition to BE and EME.
elSohly
M A
MA
ElSohly Laboratories, Incorporated, Oxford, Mississippi, USA.
Kopycki
W J
WJ
Feng
S
S
Murphy
T P
TP
Lukey
B J
BJ
D003042:Cocaine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000493:pharmacokinetics / Q000652:urine; D019970:Cocaine-Related Disorders; D005554:Forensic Medicine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans
C085009:3-hydroxybenzoylecgonine; D003042:Cocaine