12-Hydroxyeicosatetraenoic acid upregulates P-selectin-induced tissue factor activity on monocytes.
12-Hydroxyeicosatetraenoic acid (12-HETE), a product of the platelet lipoxygenase pathway, amplifies tissue factor expression by P-selectin-stimulated monocytes in a time- and dose-dependent fashion. The same effect is observed when monocytes are incubated with Chinese hamster ovary cells transfected with the P-selectin cDNA. Both 5-HETE and leukotriene C4 are inactive in this system. Furthermore, the effect is not dependent on non-specific monocyte adhesion, since monocytes incubated with CHO cells expressing E-selectin do not express tissue factor, either in the presence or in the absence of 12-HETE. These results show that 12-HETE is a cofactor for the expression of tissue factor by monocytes.
Pellegrini
G
G
Department of Vascular Medicine and Pharmacology, Istituto Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, S. Maria Imbaro, Italy.
Malandra
R
R
Celi
A
A
Furie
B C
BC
Furie
B
B
Lorenzet
R
R
D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid / Q000493:pharmacokinetics / Q000494:pharmacology*; D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism*; D013925:Thromboplastin / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects*
D019007:P-Selectin; D019377:12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; D013925:Thromboplastin