Long term potentiation of single WDR neurons in spinalized rats.
We report long-term potentiation (LTP) in single spinal wide dynamic range (WDR) neurons in urethane-anaesthetized spinalized rats with a complete neuromuscular blockade. Peripheral influences were excluded by a complete lidocaine block distal to the stimulation site on the sciatic nerve. As previously shown A-beta fibre evoked responses were not increased by the tetanic stimulation when there was a neuromuscular blockade during the experiment. Spinalization, excluding influences from supraspinal structures, increased all firing responses, and the LTP of C-fibre evoked responses when calculated in number of action potentials compared to intact animals and to previous studies. Furthermore, an LTP of the post discharge was observed after spinalization. An LTP of the post discharge has previously not been reported. Therefore, we conclude that LTP in the dorsal horn normally seems to be inhibited by descending pathways.
Svendsen
F
F
Department of Physiology, University of Bergen, Norway, Arstadveien 19, N-5009, Bergen, Norway. frode.svendsen@pki.uib.no
Tjølsen
A
A
Gjerstad
J
J
Hole
K
K
D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D003655:Decerebrate State; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000502:physiology; D005260:Female; D006339:Heart Rate / Q000502:physiology; D017774:Long-Term Potentiation; D009407:Nerve Block; D009412:Nerve Fibers / Q000502:physiology; D019148:Neuromuscular Blockade; D009474:Neurons / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology / Q000502:physiology*