Preparative purification of a high-mannose type N-glycan from soy bean agglutinin by hydrazinolysis and tyrosinamide derivatization.
The N-linked oligosaccharide from soy bean agglutinin (Man9) was isolated on a preparative scale following derivatization with Boc-tyrosine. The procedure utilized preparative hydrazinolysis to release the oligosaccharide and yielded multi-micromol quantities of Boc-tyrosine-Man9 which was characterized by 1H NMR and ES-MS.
Evers
D L
DL
Division of Medicinal Chemistry and Division of Pharmaceutics, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, Michigan, 48109-1065, USA.
Hung
R L
RL
Thomas
V H
VH
Rice
K G
KG
D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D006834:Hydrazines / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008358:Mannose / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D037121:Plant Lectins; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D030262:Soybean Proteins; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000737:chemistry; D010835:Phytohemagglutinins
D006834:Hydrazines; D037102:Lectins; D008358:Mannose; D037121:Plant Lectins; D011134:Polysaccharides; D030262:Soybean Proteins; D014443:Tyrosine; C028824:soybean lectin; D010835:Phytohemagglutinins; C036387:tyrosinamide; C029424:hydrazine