A bacteriocin-mediated antagonism by Enterococcus faecium BC25 against ruminal Streptococcus bovis.
A bacteriocin-like activity produced by Enterococcus faecium BC25, isolated from the the rumen of a cow, was partially purified and characterized. The active substance was prepared by ammonium sulfate and chloroform/methanol precipitation of culture supernatant. The bacteriocin was a protein of molecular mass 17.5 kDa. Activity was inactivated by trypsin and proteinase K. The bacteriocin BC25 inhibited growth of amylolytic ruminal strains of Streptococcus bovis, including S. bovis AO 24/85. Results showed that bacteriocine substance BC25 has a bacteriostatic effect when present in concentrations exceeding 125 AU/ml. Agar overlays and batch culture growth experiments proved that E. faecium BC25 was producing bacteriocin that inhibited the growth of S. bovis.
Morovský
M
M
Department of Biochemistry, Faculty of Sciences, P.J. Safárik University, Kosice, Slovakia.
Pristas
P
P
Czikková
S
S
Javorský
P
P
D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D002417:Cattle; D011232:Chemical Precipitation; D002725:Chloroform / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D019286:Endopeptidase K / Q000737:chemistry; D016984:Enterococcus faecium / Q000378:metabolism*; D000432:Methanol / Q000737:chemistry; D008970:Molecular Weight; D009391:Nephelometry and Turbidimetry; D012417:Rumen / Q000382:microbiology*; D016985:Streptococcus bovis / Q000254:growth & development*; D014357:Trypsin / Q000737:chemistry
D001430:Bacteriocins; D002725:Chloroform; D014357:Trypsin; D019286:Endopeptidase K; D000645:Ammonium Sulfate; D000432:Methanol