The role of oxidative stress in rhinovirus induced elaboration of IL-8 by respiratory epithelial cells.
A direct correlation has been reported between the severity of symptoms associated with rhinovirus infection and the concentration of interleukin-8 in nasal secretions. The purpose of these studies was to examine the mechanism of rhinovirus-induced IL-8 elaboration. Rhinovirus infection induced oxidative stress in Beas-2b cells and the concentration of H2O2 in supernatant media from rhinovirus challenged cells was 12.5 +/- 6.1 microM 1 h after challenge compared to 0.7 +/- 0.3 microM in supernatant from control cells. N-acetyl cysteine inhibited RV-induced NF-kappaB activation and IL-8 elaboration. IL-8 concentrations were 36 +/- 2 pg/ml and 10 +/- 1 pg/ml 6 h after virus challenge in untreated and NAC-treated (30 mM NAC) cells, respectively. Despite the effects of NAC on IL-8 elaboration and NF-kappaB activation, RV stimulated increases in supernatant H2O2 were not altered by NAC. These data suggest that RV stimulation of IL-8 in respiratory epithelium is mediated through production of oxidative species and the subsequent activation of NF-kappaB.
Biagioli
M C
MC
Department of Pediatrics, Medical University of South Carolina, Charelston 29425, USA.
Kaul
P
P
Singh
I
I
Turner
R B
RB
D000111:Acetylcysteine / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016209:Interleukin-8 / Q000096:biosynthesis*; D008168:Lung / Q000166:cytology / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000502:physiology*; D012229:Rhinovirus / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology; D013268:Stimulation, Chemical
D016209:Interleukin-8; D016328:NF-kappa B; D006861:Hydrogen Peroxide; D000111:Acetylcysteine