Susceptibility of human lymphoblastoid B-cell line CE to HIV1 infection.
In this preliminary report, we provide evidence that the human B-lymphoblastoid cell line (LCL) CE, bone-marrow-derived, previously reported to be permissive to hepatitis C virus, is also permissive to HIV1 infection. HIV1 genomes were detectable in cell supernatants, virus RNA transcripts and proviral DNAs in cell extracts at different times post-infection. Therefore, we propose this LCL cell line as a tool for exploring the mutual interactions of the two viruses in double-infected cells.
Valli
M B
MB
Istituto di Medicina Sperimentale, Roma.
Carloni
G
G
Bertolini
L
L
D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D016679:Genome, Viral; D016655:HIV Core Protein p24 / Q000032:analysis; D054303:HIV Reverse Transcriptase / Q000032:analysis; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D014779:Virus Replication
D016655:HIV Core Protein p24; D012367:RNA, Viral; D054303:HIV Reverse Transcriptase