Studies on Alismatis Rhizoma. II. Anti-complementary activities of methanol extract and terpene components from Alismatis Rhizoma (dried rhizome of Alisma orientale).
A methanol extract (TMe-ext) from the dried rhizome of Alisma orientale was screened for anti-complementary activity in experimental models. In the animal models, it was found that TMe-ext inhibits zymosan-induced hind paw edema in rats and zymosan-activated rat serum (ZAS)-induced vascular permeability in mice. TMe-ext showed an inhibitory effect on complement-induced hemolysis through both the classical pathway and the alternative pathway. And TMe-ext inhibited hypotonic shock-induced hemolysis, but this effect was weak compared with the anti-complementary activities of TMe-ext. Four triterpenes (alisol A, alisol A monoacetate, alisol B and alisol B monoacetate) isolated from the rhizome also inhibited the complement-induced hemolysis through the classical pathway, but two sesquiterpenes (alismol and alismoxide) were ineffective. These results indicate that Alismatis Rhizoma shows anti-complementary activity, and its anti-complementary components are partially attributable to the terpene components mentioned above.
Matsuda
H
H
Faculty of Pharmaceutical Sciences, Kinki University, Higashiosaka, Osaka, Japan.
Tomohiro
N
N
Yoshikawa
M
M
Kubo
M
M
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002199:Capillary Permeability / Q000187:drug effects; D003169:Complement Inactivator Proteins / Q000494:pharmacology*; D003170:Complement Pathway, Alternative; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D004487:Edema / Q000139:chemically induced / Q000517:prevention & control; D006461:Hemolysis / Q000187:drug effects; D007038:Hypotonic Solutions; D019684:Magnoliopsida / Q000737:chemistry; D008297:Male; D000432:Methanol; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D013729:Terpenes / Q000494:pharmacology* / Q000627:therapeutic use; D015054:Zymosan
D018926:Anti-Allergic Agents; D003169:Complement Inactivator Proteins; D004365:Drugs, Chinese Herbal; D007038:Hypotonic Solutions; D010936:Plant Extracts; D013729:Terpenes; D015054:Zymosan; D000432:Methanol