title: Identification and cloning of rat galectin-2: expression is predominantly in epithelial cells of the stomach.
abstract: A complementary DNA clone preferentially expressed in the gastrointestinal tract was obtained from a rat stomach library. The protein coded by the clone had a single carbohydrate recognition domain having conserved motifs for beta-galactoside binding and showed 67% amino acid identity with human galectin-2. The recombinant protein synthesized in Escherichia coli could bind to an asialofetuin column and was eluted with beta-galactopyranoside. From these observations, we named the protein rat galectin-2 coded by the cDNA. The rat galectin-2 was predominantly expressed in the epithelial cells of stomach. Thus this protein may form a mucin layer cross-linking with the beta-galactoside moiety of glycoproteins.
authors.affiliation: Institute of Scientific and Industrial Research, Osaka University, Osaka, Ibaraki, 567-0047, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Oka
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: Y
authors.initials: Y
authors.lastname: Arata
authors.forename: J
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: K
authors.initials: K
authors.lastname: Kasai
authors.forename: Y
authors.initials: Y
authors.lastname: Wada
authors.forename: M
authors.initials: M
authors.lastname: Futai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000378:metabolism*; D037501:Galectin 2; D005753:Gastric Mucosa / Q000378:metabolism*; D005786:Gene Expression Regulation; D006388:Hemagglutinins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D037102:Lectins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid; D013270:Stomach / Q000166:cytology
chemical_list: D037501:Galectin 2; D006388:Hemagglutinins; D037102:Lectins; D011994:Recombinant Proteins