title: A yeast artificial chromosome-based physical map of the juvenile amyotrophic lateral sclerosis (ALS2) critical region on human chromosome 2q33-q34.
abstract: The autosomal recessive form of juvenile amyotrophic lateral sclerosis (ALS2; RFALS Type 3) has previously been mapped to the 8-cM interval flanked by D2S115 and D2S155 on human chromosome 2q33-q34. We have established a yeast artificial chromosome (YAC) contig spanning an approximately 8-Mb region of the ALS2 candidate region and mapped 52 transcribed DNA sequences including 13 known genes and 39 expressed sequenced tags within this YAC contig. The establishment of a YAC contig and transcript map that spans the region containing the ALS2 mutation is an essential step in the identification of the ALS2 gene.
authors.affiliation: Department of Medical Genetics and Centre for Molecular Medicine and Therapeutics, University of British Columbia, 3024-980 West 28th Avenue, Vancouver, British Columbia, V5Z 4H4, Canada.
authors.forename: S
authors.initials: S
authors.lastname: Hadano
authors.forename: K
authors.initials: K
authors.lastname: Nichol
authors.forename: R R
authors.initials: RR
authors.lastname: Brinkman
authors.forename: J
authors.initials: J
authors.lastname: Nasir
authors.forename: D
authors.initials: D
authors.lastname: Martindale
authors.forename: B F
authors.initials: BF
authors.lastname: Koop
authors.forename: D W
authors.initials: DW
authors.lastname: Nicholson
authors.forename: S W
authors.initials: SW
authors.lastname: Scherer
authors.forename: J E
authors.initials: JE
authors.lastname: Ikeda
authors.forename: M R
authors.initials: MR
authors.lastname: Hayden
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D020451:Contig Mapping; D006801:Humans