Regulation of selected biological processes in neoplastic cell lines by halogen derivatives of 5-hydroxy-2-hydroxymethyl-4-pyranone.
The present study was undertaken to investigate the effects of eight halogen derivatives of 5-hydroxy-2-hydroxymethyl-4-pyranone on a) neoplastic cell growth, b) DNA, RNA, and protein synthesis, c) cytoplasmic phosphorylation, and d) cAMP accumulation in culture medium. Two neoplastic cell lines (the murine leukemia L1210 and the rat pituitary GH4C1) were used in the present study. The inhibitory effects of the halogen derivatives on GH4C1 cell division when compared to L1210 cells were found to be almost equal. Two halogen derivatives (2-chloromethyl-5-hydroxy-4-pyranone and 6-bromo-5-hydroxy-2-hydroxymethyl-4-pyranone) were found to inhibit DNA, RNA and protein synthesis. Moreover, the electrophoretic profile of low molecular mass cytoplasmic proteins was markedly influenced by certain halogen derivatives of 5-hydroxy-hydroxymethyl-4-pyranone in L1210 cells.
Bransová
J
J
Institute of Experimental Endocrinology, Slovak Academy of Sciences, Bratislava, Slovak Republic. ueenbran@savba.savba.sk
Uher
M
M
Brtko
J
J
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism; D004273:DNA, Neoplasm / Q000096:biosynthesis; D007939:Leukemia L1210; D051379:Mice; D015394:Molecular Structure; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010911:Pituitary Neoplasms; D011753:Pyrones / Q000737:chemistry / Q000633:toxicity*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D051381:Rats; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D011753:Pyrones; D012334:RNA, Neoplasm; C116987:6-bromo-5-hydroxy-2-hydroxymethyl-4-pyranone; D000242:Cyclic AMP; C116986:2-chloromethyl-5-hydroxy-4-pyranone