Liquid chromatography and electrospray mass spectrometric mapping of peptides from human plasma filtrate.
We present a multidimensional approach to map the composition of complex peptide mixtures obtained as crude extract from biological liquids by (1) cation exchange chromatography and (2) subsequent microbore reversed-phase liquid chromatography and electrospray mass spectrometry coupling (LC-MS). Human hemofiltrate is an equivalent to blood and is used to obtain peptide material in large quantities from patients with chronic renal failure. The upper exclusion limit of the filtration membranes used results in a protein-free filtrate containing peptides in a range up to 20 ku. Using this unique peptide source, several thousand peptides were detected and an LC-MS data base of circulating human peptides was created. The search for known peptides by their molecular mass is a reliable method to guide peptide purification.
Raida
M
M
Lower Saxony Institute for Peptide Research, Hannover, Germany. mraida@gmx.de
Schulz-Knappe
P
P
Heine
G
G
Forssmann
W G
WG
D002852:Chromatography, Ion Exchange; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism; D013058:Mass Spectrometry; D008970:Molecular Weight; D010449:Peptide Mapping / Q000379:methods*; D010455:Peptides / Q000097:blood*
D010455:Peptides