Interactions between beta2-adrenoreceptor agonist and NO donors in the relaxation of guinea pig trachea in vitro.
The effects of interaction between the beta2-adrenoreceptor agonist, salbutamol, and the nitric oxide donors, sodium nitroprusside and 3-morpholinosydnonimine (SIN-1), on guinea pig trachea contraction were studied. Cumulatively increased concentrations (0.1-10 nM) of salbutamol together with a single concentration of SNP (0.33 microM) or with SIN-1 (1 microM) showed significant (p < 0.001) synergy for the inhibition of 1 microM metacholine-induced contraction. Significant synergy (p < 0.05) was also found for the inhibition of this contraction by cumulatively increased concentrations (0.1-33 microM) of SNP and a single concentration (1 nM) of salbutamol. No synergistic effect was found on the 40 mM KC1-induced contraction. We suggest that the combination of NO donors with salbutamol has a synergistic effect on metacholine contraction.
Vaali
K
K
Institute of Biomedicine, Department of Pharmacology and Toxicology, University of Helsinki, Finland.
Li
L
L
Vapaatalo
H
H
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D009126:Muscle Relaxation / Q000187:drug effects*; D020030:Nitric Oxide Donors / Q000494:pharmacology*; D014132:Trachea / Q000187:drug effects* / Q000502:physiology
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D020030:Nitric Oxide Donors