Differentiation elicits negative regulation of human beta-galactoside alpha2,6-sialyltransferase at the mRNA level in the HL-60 cell line.
We studied the regulation of the beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) gene during HL-60 cell differentiation induced with dimethyl sulfoxide (DMSO), all transretinoic acid (ATRA), and phorbol myristate acetate (PMA). During HL-60 cell line differentiation, cell surface levels of alpha2,6-sialic acids expression decreased, as measured by flow cytometric analysis using Sambucus nigra agglutinin (SNA). Activities of hST6Gal I and levels of hST6Gal I mRNA dramatically decreased after 1 day of stimulation. Using reverse transcription polymerase chain reaction (PT-PCR), we found the major hST6Gal I mRNA isoform in HL-60 cells contains 5'-untranslated exons Y and Z. These results suggest that the expression of cell surface alpha2,6-sialic acids is controlled at the mRNA level, which is regulated by a promoter located 5'-upstream of exon Y.
Taniguchi
A
A
Department of Clinical Chemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan. taniaki@phar.toho-u.ac.jp
Higai
K
K
Hasegawa
Y
Y
Utsumi
K
K
Matsumoto
K
K
D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002462:Cell Membrane / Q000378:metabolism; D017931:DNA Primers; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005091:Exons; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D018922:HL-60 Cells; D006801:Humans; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics*; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
D017931:DNA Primers; D012333:RNA, Messenger; D012794:Sialic Acids; D014212:Tretinoin; D012799:Sialyltransferases; D013755:Tetradecanoylphorbol Acetate; D004121:Dimethyl Sulfoxide; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase