Peroral administration of 14C-poly(D,L-lactic acid) nanoparticles coated with human serum albumin or polyvinyl alcohol to guinea pigs.
Biodegradable 14C-poly(D,L-lactic acid) (PLA50) nanoparticles coated either with a readily digestible protein albumin or with a non-digestible coating agent, polyvinyl alcohol (PVA), were prepared by the solvent evaporation technique. The nanoparticles were administered perorally to guinea pigs to evaluate the gastro-intestinal degradation of their PLA50 matrix. In the case of PLA50 nanoparticles coated with digestible albumin, substantial gastro-intestinal degradation of the PLA50 matrix occurred, leading to the passage of considerable amount (> or =45%) of water-soluble products across the gastrointestinal barrier. When a non-digestible coating agent like PVA was used, the degradation of the PLA50 matrix in the gastro-intestinal tract was at least two times lower (> or =19%). The results show that it is possible to control the in vivo degradation of PLA50 nanoparticles using appropriate coating agents. The present investigations showed a good correlation between previously observed in vitro results and the in vivo findings.
Landry
F B
FB
Institut für Pharmazeutische Technologie der Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
Bazile
D V
DV
Spenlehauer
G
G
Veillard
M
M
Kreuter
J
J
D000818:Animals; D001345:Autoradiography; D002245:Carbon Dioxide / Q000378:metabolism; D002850:Chromatography, Gel; D004337:Drug Carriers; D005243:Feces / Q000737:chemistry; D006168:Guinea Pigs; D006801:Humans; D007408:Intestinal Absorption; D019344:Lactic Acid / Q000493:pharmacokinetics*; D008297:Male; D008863:Microspheres; D010316:Particle Size; D011091:Polyesters; D011108:Polymers / Q000493:pharmacokinetics*; D011142:Polyvinyl Alcohol / Q000378:metabolism; D012709:Serum Albumin / Q000378:metabolism; D014018:Tissue Distribution
D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D012709:Serum Albumin; D002245:Carbon Dioxide; D019344:Lactic Acid; C033616:poly(lactide); D011142:Polyvinyl Alcohol