title: Persistence of human papillomavirus DNA in cervical lesions after treatment with diathermic large loop excision.
abstract: OBJECTIVE
  The aim of this study was to identify human papillomavirus (HPV) in cervical intraepithelial neoplasia (CIN) lesions and to evaluate the persistence of viral DNA after diathermic large loop excision (DLLE) treatment.
  
  
  METHODS
  Biopsies from 36 patients with low- and high-grade CIN lesions were studied before and after DLLE treatment looking for HPV sequences. DNA was extracted to perform a radioactive polymerase chain reaction (PCR) using GP 5,6 generic primers. PCR products were analyzed by the single-stranded conformational polymorphism (SSCP) which is a simultaneous detection and typing method. Dot-blot hybridization with generic and type-specific biotinylated oligonucleotide probes was applied in some cases.
  
  
  RESULTS
  HPV DNA was found in all pretreatment samples, and the viral type was identified in 80% of them, HPV 16 being the most prevalent. The viral type coincided with that detected in the first biopsy in all except one case. Seventy five percent of the patients (27 cases) were negative for CIN at follow up, but 50% of them remained HPV DNA positive.
  
  
  CONCLUSIONS
  DLLE treatment was effective in removing the CIN lesion but not the HPV. This fact points out the need to asses the presence of HPV in DNA during the follow-up, since viral persistence has been considered a high risk factor for recurrence and/or malignant transformation.
authors.affiliation: Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina. oncovir@sudnet.com.ar
authors.forename: A L
authors.initials: AL
authors.lastname: Distéfano
authors.forename: M A
authors.initials: MA
authors.lastname: Picconi
authors.forename: L V
authors.initials: LV
authors.lastname: Alonio
authors.forename: D
authors.initials: D
authors.lastname: Dalbert
authors.forename: J
authors.initials: J
authors.lastname: Mural
authors.forename: O
authors.initials: O
authors.lastname: Bartt
authors.forename: G
authors.initials: G
authors.lastname: Bazán
authors.forename: G
authors.initials: G
authors.lastname: Cervantes
authors.forename: M
authors.initials: M
authors.lastname: Lizano
authors.forename: A G
authors.initials: AG
authors.lastname: Carrancá
authors.forename: A
authors.initials: A
authors.lastname: Teyssié
mesh_terms: D000293:Adolescent; D000328:Adult; D001706:Biopsy; D015344:DNA Probes, HPV / Q000737:chemistry; D004279:DNA, Viral / Q000032:analysis / Q000737:chemistry; D004564:Electrocoagulation; D005260:Female; D006801:Humans; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000254:growth & development*; D030361:Papillomavirus Infections / Q000601:surgery* / Q000821:virology; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D014412:Tumor Virus Infections / Q000601:surgery* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000601:surgery / Q000821:virology*
chemical_list: D015344:DNA Probes, HPV; D004279:DNA, Viral