[Registration of stable aberrations in peripheral blood lymphocytes using G-differential chromosome staining and fluorescence in situ hybridization methods].
G-banding analysis and fluorescence in situ hybridization (FISH) for whole chromosomes 1, 2 and 4 were applied in comparative assay for frequency of stable chromosome aberrations in 37 individuals with previous exposure to radiation (15 clean-up workers/liquidators of Chernobyl nuclear power plant accident and 22 residents of radiocontaminated areas) and in 18 individuals of a reference group. In G-banding analysis of stable aberrations, we used classification of Ohtaki K. et al., 1992, in compliance with ISCN, 1985. FISH-assay for translocations is performed in accordance with classification of Tucker J.D. et al., 1995. Comparison of the results reveals statistical trustworthy correlation between the two assays. The results point out that FISH for translocations in as few as three chromosomes, when combined with screening of numerous metaphases, provides sensitivity comparable with that provided by G-banding which covers the whole genome.
Obukhova
T N
TN
National Research Center for Hematology, Russian Academy of Medical Sciences, Moscow.
Domracheva
E V
EV
D000328:Adult; D000368:Aged; D000398:Air Pollution, Radioactive; D002478:Cells, Cultured; D002648:Child; D002869:Chromosome Aberrations; D002871:Chromosome Banding; D002875:Chromosomes / Q000528:radiation effects*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics / Q000528:radiation effects; D002889:Chromosomes, Human, Pair 2 / Q000235:genetics / Q000528:radiation effects; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics / Q000528:radiation effects; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects; D008297:Male; D008677:Metaphase / Q000235:genetics; D008875:Middle Aged; D008962:Models, Theoretical; D011210:Power Plants; D018788:Radioactive Hazard Release; D014178:Translocation, Genetic; D014455:Ukraine