title: PRC1: a human mitotic spindle-associated CDK substrate protein required for cytokinesis.
abstract: We have identified a novel human protein, PRC1, that is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 has sequence homology to the budding yeast anaphase spindle elongation factor Ase1p. Like Ase1p, PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. Microinjection of anti-PRC1 antibodies into HeLa cells blocked cellular cleavage, but not nuclear division, indicating a functional role for PRC1 in the process of cytokinesis.
authors.affiliation: Salk Institute, La Jolla, California 92037, USA. wjiang@salk.edu
authors.forename: W
authors.initials: W
authors.lastname: Jiang
authors.forename: G
authors.initials: G
authors.lastname: Jimenez
authors.forename: N J
authors.initials: NJ
authors.lastname: Wells
authors.forename: T J
authors.initials: TJ
authors.lastname: Hope
authors.forename: G M
authors.initials: GM
authors.lastname: Wahl
authors.forename: T
authors.initials: T
authors.lastname: Hunter
authors.forename: R
authors.initials: R
authors.lastname: Fukunaga
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites / Q000235:genetics; D002453:Cell Cycle / Q000502:physiology; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D003001:Cloning, Molecular; D018844:Cyclin-Dependent Kinases / Q000378:metabolism; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D008941:Spindle Apparatus / Q000378:metabolism; D013379:Substrate Specificity; D013912:Threonine / Q000235:genetics
chemical_list: D018797:Cell Cycle Proteins; D018076:DNA, Complementary; C116662:PRC1 protein, human; D013912:Threonine; D018844:Cyclin-Dependent Kinases