title: [The effects of low-dose Co-60 irradiation on the course of aseptic arthritis in a rabbit knee joint].
abstract: OBJECTIVE
  Numerous clinical observations demonstrate the efficacy of low radiation doses in the treatment of painful osteoarthritis. Experimental investigations remain scarce. We investigated the effects of locally daily 5 times 1.0 Gy 60-Co irradiation on an artificially induced aseptic gonarthritis in rabbits.
  
  
  METHODS
  Three separate experiments (EV) were performed (10 rabbits per experiment, 5 treated/5 controls; duration: EV1: 18 days; EV2: 6 days; EV3: 29 days). An aseptic arthritis in the right knee joint of rabbits was induced by intraarticular injection of 0.5 ml papain solution (3%, 30,000 USP/mg) on day 0. The arthritic knee joint of the anesthesized animals was irradiated daily from day 1 to 5 with 5 times 1.0 Gy. The controls were sham-irradiated under the same conditions. The time course of arthritis in treated animals and sham-treated controls was evaluated by clinical, laboratory-chemical and histological criteria. The clinical investigation was performed daily, the puncture of the knee-joints was carried out several times in EV1, and at the end of experiments in EV2 and EV3. At the end of the observation period, animals were killed and the knee joints excised for histological analysis.
  
  
  RESULTS
  The intraarticular injection of papain caused a peracute inflammatory response in all animals. After 1 week the chronic stage was reached, and the experimental arthritis resolved slowly within several weeks. Local irradiation accelerated the decrease of inflammatory joint swelling, being significant by day 4. On day 6 the volume of synovial fluid in irradiated knee-joints was significantly smaller. The morphometric data indicated a reduction in thickness of synovial membrane, a decrease in number of synovial cell layers, and a decrease in distance between capillaries and the synovial membrane surface following irradiation of arthritic joints. Due to considerable individual variability, the morphometric data partially did not reach statistically significance.
  
  
  CONCLUSIONS
  The experiments provide evidence for an antiphlogistic effect of irradiation with 5 times 1.0 Gy in vivo. They support the clinical observations of the efficacy of anti-inflammatory radiotherapy.
authors.affiliation: Veterinärmedizinische Fakultät, Universität Leipzig.
authors.forename: U
authors.initials: U
authors.lastname: Fischer
authors.forename: F
authors.initials: F
authors.lastname: Kamprad
authors.forename: F
authors.initials: F
authors.lastname: Koch
authors.forename: E
authors.initials: E
authors.lastname: Ludewig
authors.forename: R
authors.initials: R
authors.lastname: Melzer
authors.forename: G
authors.initials: G
authors.lastname: Hildebrandt
mesh_terms: D000818:Animals; D001168:Arthritis / Q000139:chemically induced / Q000473:pathology / Q000532:radiotherapy*; D003037:Cobalt Radioisotopes / Q000627:therapeutic use*; D004195:Disease Models, Animal; D006614:Hindlimb; D007719:Knee Joint / Q000473:pathology / Q000528:radiation effects*; D010206:Papain; D011817:Rabbits; D011867:Radioisotope Teletherapy / Q000379:methods* / Q000706:statistics & numerical data; D011879:Radiotherapy Dosage; D018709:Statistics, Nonparametric; D013582:Synovial Fluid / Q000737:chemistry / Q000166:cytology / Q000528:radiation effects; D013583:Synovial Membrane / Q000473:pathology / Q000528:radiation effects; D013997:Time Factors
chemical_list: D003037:Cobalt Radioisotopes; D010206:Papain