title: Keratolimbal allograft: recommendations for tissue procurement and preparation by eye banks, and standard surgical technique.
abstract: OBJECTIVE
  To facilitate increased use of keratolimbal allograft transplantation (KLAL) for severe ocular surface disease (OSD) by informing clinicians and eye banks of differences in tissue requirements and preparation for the procedure and to describe the surgical technique of KLAL transplantation.
  
  
  METHODS
  The protocol of the Minnesota Lions Eye Bank is described for procuring and preparing tissue for KLAL transplantation. The surgical technique is described in detail.
  
  
  RESULTS
  Over the last several years, we have modified our eye-banking procedures as well as our surgical technique to improve the success of KLAL transplantation.
  
  
  CONCLUSIONS
  KLAL transplantation for patients with severe ocular-surface disease is an important management option. Success is influenced by the ability to obtain appropriate tissue; this requires clinicians and eye banks to expand current procurement guidelines.
authors.affiliation: Cornea and External Disease, Davis Duehr Dean Clinic, and Department of Ophthalmology, University of Wisconsin, Madison, USA.
authors.forename: C R
authors.initials: CR
authors.lastname: Croasdale
authors.forename: G S
authors.initials: GS
authors.lastname: Schwartz
authors.forename: J V
authors.initials: JV
authors.lastname: Malling
authors.forename: E J
authors.initials: EJ
authors.lastname: Holland
mesh_terms: D017690:Cell Transplantation; D003316:Corneal Diseases / Q000601:surgery*; D019573:Epithelium, Corneal / Q000637:transplantation*; D005125:Eye Banks / Q000592:standards*; D006801:Humans; D016850:Limbus Corneae; D013508:Ophthalmologic Surgical Procedures / Q000592:standards*; D017410:Practice Guidelines as Topic; D014019:Tissue Donors; D014021:Tissue Preservation; D009927:Tissue and Organ Procurement / Q000592:standards*; D014184:Transplantation, Homologous