title: Thymidylate synthase protein and p53 mRNA form an in vivo ribonucleoprotein complex.
abstract: A thymidylate synthase (TS)-ribonucleoprotein (RNP) complex composed of TS protein and the mRNA of the tumor suppressor gene p53 was isolated from cultured human colon cancer cells. RNA gel shift assays confirmed a specific interaction between TS protein and the protein-coding region of p53 mRNA, and in vitro translation studies demonstrated that this interaction resulted in the specific repression of p53 mRNA translation. To demonstrate the potential biological role of the TS protein-p53 mRNA interaction, Western immunoblot analysis revealed nearly undetectable levels of p53 protein in TS-overexpressing human colon cancer H630-R10 and rat hepatoma H35(F/F) cell lines compared to the levels in their respective parent H630 and H35 cell lines. Polysome analysis revealed that the p53 mRNA was associated with higher-molecular-weight polysomes in H35 cells compared to H35(F/F) cells. While the level of p53 mRNA expression was identical in parent and TS-overexpressing cell lines, the level of p53 RNA bound to TS in the form of RNP complexes was significantly higher in TS-overexpressing cells. The effect of TS on p53 expression was also investigated with human colon cancer RKO cells by use of a tetracycline-inducible system. Treatment of RKO cells with a tetracycline derivative, doxycycline, resulted in 15-fold-induced expression of TS protein and nearly complete suppression of p53 protein expression. However, p53 mRNA levels were identical in transfected RKO cells in the absence and presence of doxycycline. Taken together, these findings suggest that TS regulates the expression of p53 at the translational level. This study identifies a novel pathway for regulating p53 gene expression and expands current understanding of the potential role of TS as a regulator of cellular gene expression.
authors.affiliation: Department of Medicine and Pharmacology, Yale Cancer Center and VA CT Cancer Center, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
authors.forename: E
authors.initials: E
authors.lastname: Chu
authors.forename: S M
authors.initials: SM
authors.lastname: Copur
authors.forename: J
authors.initials: J
authors.lastname: Ju
authors.forename: T M
authors.initials: TM
authors.lastname: Chen
authors.forename: S
authors.initials: S
authors.lastname: Khleif
authors.forename: D M
authors.initials: DM
authors.lastname: Voeller
authors.forename: N
authors.initials: N
authors.lastname: Mizunuma
authors.forename: M
authors.initials: M
authors.lastname: Patel
authors.forename: G F
authors.initials: GF
authors.lastname: Maley
authors.forename: F
authors.initials: F
authors.lastname: Maley
authors.forename: C J
authors.initials: CJ
authors.lastname: Allegra
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D005786:Gene Expression Regulation; D016158:Genes, p53; D006801:Humans; D046911:Macromolecular Substances; D011132:Polyribosomes / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D051381:Rats; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism*; D013940:Thymidylate Synthase / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection
chemical_list: D017931:DNA Primers; D046911:Macromolecular Substances; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D013940:Thymidylate Synthase