title: Monoclonal antibody ONS-M21 recognizes integrin alpha3 in gliomas and medulloblastomas.
abstract: The monoclonal antibody ONS-M21 recognizes an antigen found on the surface of glioma and medulloblastoma cells but does not react with the antigens of normal brain tissue. We purified and identified the 140-kDa protein by means of an antibody-binding affinity column. This 140-kDa antigen has sequences homologous to the amino-terminal region and five parts of the internal domain of integrin alpha3. When the integrin alpha3-related sequences was amplified and used to analyse the mRNA of glioma and medulloblastoma surgical specimens, the transcription level of integrin alpha3 mRNA appeared to be quantitatively correlated with the grade of malignancy. These findings suggest that the ONS-M21 antibody, which reacts with integrin alpha3, might be useful in the diagnosis of gliomas and medulloblastomas.
authors.affiliation: Department of Neurosurgery, Osaka University Medical School, Suita, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Kishima
authors.forename: K
authors.initials: K
authors.lastname: Shimizu
authors.forename: K
authors.initials: K
authors.lastname: Tamura
authors.forename: Y
authors.initials: Y
authors.lastname: Miyao
authors.forename: E
authors.initials: E
authors.lastname: Mabuchi
authors.forename: E
authors.initials: E
authors.lastname: Tominaga
authors.forename: J
authors.initials: J
authors.lastname: Matsuzaki
authors.forename: T
authors.initials: T
authors.lastname: Hayakawa
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000276:immunology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000276:immunology; D002528:Cerebellar Neoplasms / Q000175:diagnosis / Q000276:immunology; D005910:Glioma / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D039422:Integrin alpha3; D016023:Integrins / Q000032:analysis* / Q000276:immunology; D008527:Medulloblastoma / Q000175:diagnosis* / Q000276:immunology; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012333:RNA, Messenger / Q000032:analysis; D014407:Tumor Cells, Cultured
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000951:Antigens, Neoplasm; D039422:Integrin alpha3; D016023:Integrins; D012333:RNA, Messenger