title: Activity of the adenylyl cyclase in lymphocytes of male alcoholic patients is state dependent.
abstract: A decreased basal and/or stimulated activity of the G-protein/adenylyl cyclase (AC) system in peripheral blood cells has been proposed to represent a trait marker for alcoholism. However, AC activity may underlie state-dependent changes, which may impair a proper interpretation of AC activity measurements. Our study examined systematically the AC activity in peripheral lymphocytes of 73 male alcohol-dependent patients (according to DSM-IV criteria) at three different time points of measurement during the clinical course of detoxification (day 0 = at admission, while still ethanol-affected; day 2 = at the presumed peak of withdrawal symptoms; day E = after detoxification). Basal and stimulated (with GTPgammaS and forskolin) AC activities were measured. AC activities were compared to those of a control group of 44 healthy male age-matched volunteers. As our main finding, we detected a significant decrease in AC activity from day 0 to day 2 (during withdrawal), with lowered AC activities in a vast majority of patients. This effect resolved after detoxification, as AC activities showed a significant increase from day 2 to E. No significant difference was detected between day 0 and E in AC activities of the patients. Compared with controls, AC activities in patients were significantly lower at day 2, but not at day 0 and E. Taken together, our results indicate rapid and marked state-dependent changes of AC activities in alcohol-dependent patients during the course of detoxification. For an adequate interpretation of AC activities in alcoholic patients, their clinical status must be taken into consideration.
authors.affiliation: Department of Psychiatry, University of Mainz, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Szegedi
authors.forename: I
authors.initials: I
authors.lastname: Anghelescu
authors.forename: T
authors.initials: T
authors.lastname: Pauly
authors.forename: N
authors.initials: N
authors.lastname: Dahmen
authors.forename: M J
authors.initials: MJ
authors.lastname: Müller
authors.forename: H
authors.initials: H
authors.lastname: Wetzel
authors.forename: C
authors.initials: C
authors.lastname: Hiemke
mesh_terms: D000262:Adenylyl Cyclases / Q000097:blood*; D000328:Adult; D000368:Aged; D000430:Alcohol Withdrawal Delirium / Q000175:diagnosis / Q000201:enzymology / Q000534:rehabilitation; D000437:Alcoholism / Q000175:diagnosis / Q000201:enzymology* / Q000534:rehabilitation; D015415:Biomarkers / Q000097:blood; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D012016:Reference Values
chemical_list: D015415:Biomarkers; D000262:Adenylyl Cyclases