HSP90 has neurite-promoting activity in vitro for telencephalic and spinal neurons of chick embryos.
We purified a protein from the extract of denervated chick muscle. The protein had neurite-promoting activity in vitro for the chick telencephalic neurons and spinal neurons. Partial amino acid sequencing and immunoblotting revealed that the protein was chick heat shock protein 90 (HSP90). Commercially available bovine HSP90 and recombinant chick HSP90 expressed in Escherichia coli also showed the same activity. Since HSP10 (GroES) and HSP60 (GroEL) exhibited no activity for neurite outgrowth in the same culture, this activity was specific to HSP90 among the heat shock proteins.
Ishimoto
T
T
Department of Organic Materials, Osaka National Research Institute, Ikeda, Japan.
Kamei
A
A
Koyanagi
S
S
Nishide
N
N
Uyeda
A
A
Kasai
M
M
Taguchi
T
T
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000502:physiology*; D016501:Neurites / Q000187:drug effects / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000166:cytology* / Q000502:physiology; D013687:Telencephalon / Q000166:cytology* / Q000502:physiology
D018841:HSP90 Heat-Shock Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins