Differential display analysis of murine encephalitogenic mRNA.
Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease which can be induced by inoculation with activated CD4+ T lymphocytes specific for myelin proteins. It has been postulated that autoreactive Th1-type CD4+ cells are responsible for the lesions, whereas autoreactive Th2-type cells suppress or modulate the disease. However, the mechanisms involved in the development of inflammatory lesions and neurologic deficits in EAE have not been fully described, and probably involve a complex array of mediators and alternative or redundant pathways. To identify additional factors associated with the pathological role of T cells in EAE, we adapted the differential mRNA display technique to fingerprint the transcripts expressed by encephalitogenic and non-encephalitogenic T cells. Using 60 primer combinations, 21 differentially expressed cDNAs were identified. Among them 13 are known sequences, including IL-3 in non-encephalitogenic lymphocytes. Their relevance for the disease process is discussed.
Jeong
M C
MC
Department of Neurology, School of Medicine, University of California, San Francisco 94143, USA.
Izikson
L
L
Uccelli
A
A
Brocke
S
S
Oksenberg
J R
JR
D000818:Animals; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D015870:Gene Expression; D005786:Gene Expression Regulation; D051379:Mice; D008969:Molecular Sequence Data; D004676:Myelin Basic Protein / Q000276:immunology; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology
D018076:DNA, Complementary; D004676:Myelin Basic Protein; D012333:RNA, Messenger