title: Bacterial expression of tenecin 3, an insect antifungal protein isolated from Tenebrio molitor, and its efficient purification.
abstract: Tenecin 3, an antifungal protein isolated from the insect Tenebrio molitor larvae, inhibits growth of the fungus Candida albicans. However, the antifungal mechanism and functions of tenecin 3 have not yet been studied due to its very low availability from the natural source. Here we report an expression system of the recombinant tenecin 3 in E. coli, whose amino acid composition is the same with that of the natural tenecin 3. We also devised a simple and easy procedure to isolate the recombinant protein from the bacterial cell extracts. The recombinant tenecin 3 showed an antifungal activity against C. albicans as the natural tenecin 3 did. Therefore large quantities of tenecin 3 can be easily obtained by the expression and purification system of tenecin 3 described in this report.
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon, Korea.
authors.forename: D H
authors.initials: DH
authors.lastname: Kim
authors.forename: Y T
authors.initials: YT
authors.lastname: Lee
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
authors.forename: B L
authors.initials: BL
authors.lastname: Lee
authors.forename: B S
authors.initials: BS
authors.lastname: Choi
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001483:Base Sequence; D002176:Candida albicans / Q000166:cytology / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D013712:Tenebrio / Q000737:chemistry*
chemical_list: D000935:Antifungal Agents; D018076:DNA, Complementary; D019476:Insect Proteins; D011993:Recombinant Fusion Proteins; C097837:tenecin 3 protein, Tenebrio molitor