title: Determination of the angle between the acceptor and anticodon stems of a truncated mitochondrial tRNA.
abstract: Significant departures from the canonical (cloverleaf) secondary structure of transfer (t)RNAs can be found among the mitochondrial (m)tRNAs of higher metazoans; these mtRNAs thus pose a challenge to the concept of an invariant, L-shaped tertiary conformation for all tRNAs. For bovine mtRNASer(AGY), which lacks the entire "dihydrouridine" (dhU) arm, two distinct tertiary models have been proposed: the first model preserves the L-shaped conformation at the expense of overall size; the second model preserves the absolute distance between the 3' terminus and the anticodon loop, while allowing the acceptor-anticodon interstem angle to vary. We have tested the central predictions of these two models by performing a series of transient electric birefringence measurements on bovine mtRNASer(AGY) constructs in which the aminoacyl-acceptor and anticodon stems were each extended by approximately 70 bp. This mtRNA species is particularly amenable to analysis, since the native bovine (heart) mtRNA is completely unmodified outside of the anticodon loop. For magnesium ion concentrations above 1 mM, the interstem angle for the extended mtRNA, 120(+/-5) degrees, is approximately 50% larger than the corresponding angle for yeast tRNAPhe (70-80 degrees) under the same ionic conditions. Furthermore, the interstem angles of the two tRNAs exhibit strikingly different responses to the addition of magnesium ions: the interstem angle for yeast tRNAPhe is reduced by nearly 50 % upon addition of 2 mM magnesium ions, whereas the angle for mtRNASer(AGY) increases by about 10%. Our data thus support a central prediction of the second model; namely, that truncated mtRNAs will possess more open interstem angles. In addition, we demonstrate that birefringence amplitude data can be used to provide model-independent estimates for the interstem angles.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, CO, 80262, USA.
authors.forename: A A
authors.initials: AA
authors.lastname: Frazer-Abel
authors.forename: P J
authors.initials: PJ
authors.lastname: Hagerman
mesh_terms: D000818:Animals; D000926:Anticodon / Q000737:chemistry*; D001718:Birefringence; D002413:Cations, Divalent; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D008274:Magnesium; D008929:Mitochondria, Heart / Q000235:genetics*; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012331:RNA, Fungal / Q000737:chemistry; D000077278:RNA, Mitochondrial; D012360:RNA, Transfer, Phe / Q000737:chemistry; D012362:RNA, Transfer, Ser / Q000737:chemistry*; D015003:Yeasts / Q000235:genetics
chemical_list: D000926:Anticodon; D002413:Cations, Divalent; D012331:RNA, Fungal; D000077278:RNA, Mitochondrial; D012360:RNA, Transfer, Phe; D012362:RNA, Transfer, Ser; D012313:RNA; D008274:Magnesium