Two species of mRNAs for the fyn proto-oncogene are produced by an alternative polyadenylation.
Two mRNA species with different sizes (3.8 kb and 2.8 kb) for the fyn proto-oncogene have been noticed during Northern hybridization analysis. However, the difference between the two mRNA species has not been resolved yet. By screening a phage expression library using the monoclonal antibody (mAb) B16-5 which recognizes Src homology 3 (SH3) domains of phospholipase C-gamma and Nck, we have cloned a cDNA encoding the larger species of fyn mRNA. The size of the clone was 3.5 kb and DNA sequencing analysis of the clone showed that it was fyn expressed mainly in T-cells, fyn (T), with an untranslated region 1 kb longer than the previously reported one. The 3'-end fragment of the clone hybridized only to the larger species (3.8 kb) of fyn mRNA but not to the smaller one (2.8 kb) on Northern blot analysis. Furthermore, an additional polyadenylation signal sequence was found at the end of this clone. These results indicate that the two mRNA species for fyn are produced by alternative polyadenylation.
Lee
C
C
Institute for Molecular Biology, Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea.
Kim
M G
MG
Jeon
S H
SH
Park
D E
DE
Park
S D
SD
Seong
R H
RH
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000201:enzymology; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D011061:Poly A / Q000235:genetics*; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D051076:Proto-Oncogene Proteins c-fyn; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology; D014018:Tissue Distribution
D018076:DNA, Complementary; D007527:Isoenzymes; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D011061:Poly A; C494960:Fyn protein, mouse; D051076:Proto-Oncogene Proteins c-fyn