Activation of protein kinase C pathway by phorbol ester results in a proteoglycan synthesis increase in peritubular cells from immature rat testis.
In cultured peritubular cells (PT) from rat testis, protein kinase C (PKC) was activated by phorbol 12-myristate 13-acetate (PMA). PMA enhanced the synthesis of proteoglycans (PG) and to a lesser extent their catabolism; the stimulation of the synthesis appeared to be due to an increase in PG protein moiety production and, at the same time, to an increase in the glycanation process as revealed by the use of an exogenous acceptor, p-nitrophenyl-beta-d-xyloside. In the presence of PMA, the molecular weight of neosynthesized PG and the length of their constitutive glycosaminoglycan chains were not modified. Moreover, the distribution of proteochondroitin sulfate and proteoheparan sulfate in medium and in cell layer remained unchanged. However, PMA reduced the sulfation level of chondroitin sulfate and heparan sulfate chains, suggesting that PKC activation resulted in an independent modulation of the sugar chain formation and of the sulfate residue transfer. PMA effect on the synthesis of hyaluronan was also determined: PMA dramatically enhanced its production by PT cells.
Thiébot
B
B
Laboratoire de Biochimie et Biologie Moléculaire, IRBA, Université de Caen, Esplanade de la Paix, 14 032 Caen, Cedex, France.
Langris
M
M
Bonnamy
P J
PJ
Bocquet
J
J
D000818:Animals; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005944:Glucosamine / Q000032:analysis; D006027:Glycosides; D006820:Hyaluronic Acid / Q000096:biosynthesis; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D011509:Proteoglycans / Q000096:biosynthesis* / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D013431:Sulfates / Q000032:analysis; D013462:Sulfur Radioisotopes; D013737:Testis / Q000187:drug effects* / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014316:Tritium
D006027:Glycosides; D011509:Proteoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D014316:Tritium; C008646:4-nitrophenyl beta-D-xyloside; D006820:Hyaluronic Acid; D011493:Protein Kinase C; D005944:Glucosamine; D013755:Tetradecanoylphorbol Acetate