Function of trehalose and glycogen in cell cycle progression and cell viability in Saccharomyces cerevisiae.
Trehalose and glycogen accumulate in Saccharomyces cerevisiae when growth conditions deteriorate. It has been suggested that aside from functioning as storage factors and stress protectants, these carbohydrates may be required for cell cycle progression at low growth rates under carbon limitation. By using a mutant unable to synthesize trehalose and glycogen, we have investigated this requirement of trehalose and glycogen under carbon-limited conditions in continuous cultures. Trehalose and glycogen levels increased with decreasing growth rates in the wild-type strain, whereas no trehalose or glycogen was detected in the mutant. However, the mutant was still able to grow and divide at low growth rates with doubling times similar to those for the wild-type strain, indicating that trehalose and glycogen are not essential for cell cycle progression. Nevertheless, upon a slight increase of extracellular carbohydrates, the wild-type strain degraded its reserve carbohydrates and was able to enter a cell division cycle faster than the mutant. In addition, wild-type cells survived much longer than the mutant cells when extracellular carbon was exhausted. Thus, trehalose and glycogen have a dual role under these conditions, serving as storage factors during carbon starvation and providing quickly a higher carbon and ATP flux when conditions improve. Interestingly, the CO2 production rate and hence the ATP flux were higher in the mutant than in the wild-type strain at low growth rates. The possibility that the mutant strain requires this steady higher glycolytic flux at low growth rates for passage through Start is discussed.
Silljé
H H
HH
Department of Molecular Cell Biology, Utrecht University, 3584 CH Utrecht, The Netherlands.
Paalman
J W
JW
ter Schure
E G
EG
Olsthoorn
S Q
SQ
Verkleij
A J
AJ
Boonstra
J
J
Verrips
C T
CT
D018533:Biomass; D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D005690:Galactose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D007700:Kinetics; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000235:genetics / Q000502:physiology*; D014199:Trehalose / Q000378:metabolism*
D006003:Glycogen; D014199:Trehalose; D005690:Galactose