Quantitative determination of domperidone in rat plasma by high-performance liquid chromatography with fluorescence detection.
A sensitive and selective analytical method for the determination of domperidone in rat plasma is described. The procedure involves liquid-liquid extraction followed by reversed-phase high-performance chromatographic analysis with fluorometric detection at 282 nm for excitation and 328 nm for emission. The detection limit was 1 ng ml(-1) using 1 ml of plasma. This assay procedure should be useful for the pharmacokinetic study of domperidone in small animals such as rats.
Yamamoto
K
K
Department of Pharmacy, The University of Tokyo Hospital, Faculty of Medicine, The University of Tokyo, Japan.
Hagino
M
M
Kotaki
H
H
Iga
T
T
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000097:blood* / Q000493:pharmacokinetics; D018492:Dopamine Antagonists / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D018492:Dopamine Antagonists; D004294:Domperidone