The low-molecular-weight phosphotyrosine protein phosphatase, when overexpressed, reduces the mitogenic response to macrophage colony-stimulating factor and tyrosine phosphorylation of its receptor.
The interference of low-molecular-weight phosphotyrosine protein phosphatase with the macrophage response to macrophage colony-stimulating factor was investigated. This paper shows that this phosphatase, already known to be involved in platelet-derived growth factor receptor signaling, is physiologically expressed in murine macrophages and dephosphorylates in vitro macrophage colony-stimulating factor receptor molecules immunoprecipitated from macrophage colony-stimulating factor-stimulated macrophages. We obtained the first demonstration that a phosphotyrosine-specific protein phosphatase dephosphorylates the macrophage colony-stimulating factor receptor in vivo and reduces the mitogenic response to macrophage colony-stimulating factor. The data indicate that low-molecular-weight phosphotyrosine protein phosphatase is a negative regulator of macrophage colony-stimulating factor receptor signaling.
Rovida
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E
Istituto di Patologia Generale, Università di Firenze, Italy.
Rigacci
S
S
Paccagnini
A
A
Dello Sbarba
P
P
Berti
A
A
D016475:3T3 Cells; D000818:Animals; D002452:Cell Count; D002460:Cell Line; D004247:DNA / Q000378:metabolism; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D051379:Mice; D008934:Mitogens / Q000037:antagonists & inhibitors / Q000502:physiology*; D008970:Molecular Weight; D010766:Phosphorylation / Q000187:drug effects; D017027:Protein Tyrosine Phosphatases / Q000096:biosynthesis* / Q000502:physiology; D016186:Receptor, Macrophage Colony-Stimulating Factor / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D013936:Thymidine / Q000378:metabolism; D014162:Transfection; D014316:Tritium / Q000378:metabolism; D014443:Tyrosine / Q000037:antagonists & inhibitors / Q000378:metabolism*
D008934:Mitogens; D014316:Tritium; D014443:Tyrosine; D016173:Macrophage Colony-Stimulating Factor; D004247:DNA; D016186:Receptor, Macrophage Colony-Stimulating Factor; D017027:Protein Tyrosine Phosphatases; D013936:Thymidine