Cloning of a cDNA encoding a new calcium-binding protein from Dictyostelium discoideum and its developmental regulation.
By employing 2D-PAGE, a protein differentially expressed during the development of Dictyostelium discoideum was discovered. The full cDNA of this protein was cloned using RT-PCR. The deduced protein is composed of 166 amino acid residues containing four EF-hand domains typical for calcium-binding proteins and was named CBP3. This protein shows little amino acid sequence homology with the other calcium-binding proteins from D. discoideum except EF-hand domains. The CBP3 mRNA was absent in vegetative amebas and accumulated maximally at 6 h of the development on filters. The mRNA level decreased thereafter and disappeared after 12 h of the development, while the protein level peaked at 8 h of development and remained constant thereafter. The mobility of CBP3 on SDS gel was shifted by treatment with EGTA, confirming the Ca2+-binding activity of the protein.
Han
Y H
YH
Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, South Korea.
Kang
S O
SO
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004023:Dictyostelium / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D017386:Sequence Homology, Amino Acid
C116578:CBP3 protein, Dictyostelium discoideum; D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D015800:Protozoan Proteins