Effects of extracellular calcium concentration on protein synthesis in Aedes albopictus cells.
The influence of extracellular calcium concentration on mosquito cells was investigated in Aedes albopictus cells cultured in a medium with different amounts of calcium. Protein synthesis in cells incubated in low calcium culture medium was inhibited when compared to control cells. This inhibition was reversed by addition of calcium to the culture medium. Two calcium-induced proteins of approximately 70,000 and 80,000 daltons were detected when calcium was added to the extracellular medium of cells incubated in low calcium medium for longer than 2 h. Northern-blot analysis indicated that Hsp70 (heat shock protein of 70,000 dalton) specific mRNA is present in cells that were cultured in low calcium medium suggesting that the 70,000 dalton protein is a member of the Hsp70 family. Our results indicate that extracellular calcium concentration can modify the gene expression pattern in A. albopictus cells and the absence of calcium in the culture medium could be considered a stress factor.
Kawamura
M T
MT
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Carvalho
M G
MG
D000330:Aedes / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology*; D002999:Clone Cells; D003470:Culture Media; D005110:Extracellular Space / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D019476:Insect Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D007424:Intracellular Fluid / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D003470:Culture Media; D018840:HSP70 Heat-Shock Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D002118:Calcium