Hypotaurine disproportionation reaction: identification of products.
Hypotaurine, concentrated under reduced pressure in HCl solution, partially (30-40%) degrades into taurine (about 30%), 2-aminoethyl-2-aminoethanethiolsulfonate (about 10%) and ethanolamine. The degradation products were identified using LC/APCI-MS, NMR, amino acid analysis and various chromatographies. The identities were confirmed by comparing the HPLS, MS and NMR characteristics of authentic compounds. One of the degradation processes during concentration of HCl solution of hypotaurine is therefore a disproportionation reaction which can interfere with the experimental results, when studying hypotaurine in biological systems.
Duprè
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S
Department of Biochemical Sciences A. Rossi Fanelli, University of Roma La Sapienza, Italy. sdupre@axcasp.caspur.it
Spirito
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A
Pinnen
F
F
Sugahara
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K
Kodama
H
H
D002851:Chromatography, High Pressure Liquid; D003538:Cystamine / Q000031:analogs & derivatives / Q000032:analysis; D019856:Ethanolamine / Q000032:analysis; D006851:Hydrochloric Acid; D013058:Mass Spectrometry; D013450:Sulfones / Q000032:analysis; D013654:Taurine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism
D013450:Sulfones; C118169:cystamine thiolsulfonate; D013654:Taurine; D019856:Ethanolamine; C003949:hypotaurine; D006851:Hydrochloric Acid; D003538:Cystamine