Reconstitution of a voltage and calcium dependent potassium channel from rat cerebellum.
Membrane vesicles from rat cerebellum were reconstituted into lipid bilayers. The activity of two different potassium channels was recorded: (1) a small conducting voltage dependent potassium channel insensitive to [Ca2+]i, (2) a calcium and voltage dependent potassium channel (KCa). KCa channels had a conductance of (302+/-15) pS (n=5) and were activated by [Ca2+]i and membrane depolarizations. They were blocked by tetraethylamonium (TEA) and charybdotoxin (CTX) but insensitive to noxiustoxin (NTX). Finally, we showed the blocking effect of Androctonus australis Hector (AaH) scorpion venom on KCa channels from rat cerebellum.
Ottolia
M
M
Istituto di Cibernetica e Biofisica, CNR, Genova, Italy.
Babini
E
E
Gazzotti
P
P
Possani
L D
LD
Prestipino
G
G
D000818:Animals; D002118:Calcium / Q000502:physiology*; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism*; D018999:Charybdotoxin / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D008051:Lipid Bilayers / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D015221:Potassium Channels / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D012604:Scorpion Venoms / Q000494:pharmacology; D019789:Tetraethylammonium / Q000494:pharmacology
D008051:Lipid Bilayers; D015221:Potassium Channels; D012604:Scorpion Venoms; D018999:Charybdotoxin; D019789:Tetraethylammonium; C052567:noxiustoxin; D002118:Calcium