Cloning of caf1+, caf2+ and caf4+ from Schizosaccharomyces pombe: their involvement in multidrug resistance, UV and pH sensitivity.
We previously identified four nuclear genes (caf1+ to caf4+) in Schizosaccharomyces pombe, mutations in which can confer caffeine resistance. Here we report the cloning and sequencing of caf1+, caf2+ and caf4+. All three genes are allelic to genes (hba1+, crm1+ and trr1+, respectively) involved in multidrug resistance mechanisms or in stress response systems. In agreement with this the caffeine-resistant mutants caf1(hba1)-21, caf2(crm1)-3 and caf4(trr1)-83 are also resistant to brefeldin. Disruption of caf1(hba1)+ and caf4(trr1)+ makes cells sensitive to high pH. The overlapping ranges of pleiotropic effects and the genetic interaction detected between caf1(hba1)+ and caf2(crm1)+ suggest that the three genes function in interlinked systems.
Benko
Z
Z
Trafford Centre, University of Sussex, Falmer, Brighton, UK. peterdora@tigris.klte.hu
Sipiczki
M
M
Carr
A M
AM
D002110:Caffeine / Q000494:pharmacology; D003001:Cloning, Molecular; D004352:Drug Resistance, Microbial / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005800:Genes, Fungal; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics*; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects*; D017422:Sequence Analysis, DNA
D009687:Nuclear Proteins; D002110:Caffeine