TOFE human-B-cell-line-based adsorption-inhibition assay to detect HCV neutralizing antibodies.
We previously demonstrated that the human lymphoblastoid B-cell line (LCL) TOFE, derived from normal human bone marrow, is permissive to HCV infection. In this report we developed an in vitro HCV adsorption-inhibition assay based on TOFE cells, to reveal the presence of neutralizing antibodies in sera from acutely infected patients.
Valli
M B
MB
Istituto di Medicina Sperimentale, Rome, Italy.
Bertolini
L
L
Manzin
A
A
Clementi
M
M
Carloni
G
G
D000327:Adsorption; D001402:B-Lymphocytes / Q000821:virology*; D002460:Cell Line; D016174:Hepacivirus / Q000302:isolation & purification / Q000502:physiology*; D006526:Hepatitis C / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood* / Q000276:immunology; D006801:Humans; D009500:Neutralization Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction
D018937:Hepatitis C Antibodies