CD4/GP120mac251 interaction induces phospholipase A2 (PLA2) activation in cynomolgus monkey lymphocytes.
Cynomolgus monkey are susceptible to infection with select simian immunodeficiency virus (SIV). We investigated the early interactions between SIV envelope glycoproteins (gp120mac251) and macaque lymphocytes. Our results demonstrate that the soluble viral glycoprotein induce a specific phospholipase A2 (PLA2) activation in lymphocytes through CD4. This PLA2 activation, induced after envelope glycoprotein-CD4 interaction, because of its locally destabilizing membrane effect, may have important implications for preparing the lymphocyte membrane for fusion with the viral particle. However, this effect is not sufficient to accomplish fusion. These data indicate that the specific step of fusion may be downstream from PLA2 activation.
Mavoungou
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Unit of Immunology, International Center for Medical Research, Franceville (CIRMF), Gabon. emavoung@cirmfrv.fr
Poaty-Mavoungou
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V
D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D005260:Female; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008252:Macaca fascicularis / Q000276:immunology / Q000821:virology; D008297:Male; D008562:Membrane Glycoproteins; D008992:Monkey Diseases / Q000276:immunology; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology; D015302:Simian Immunodeficiency Virus / Q000276:immunology*; D014759:Viral Envelope Proteins
D015704:CD4 Antigens; D015699:HIV Envelope Protein gp120; D008562:Membrane Glycoproteins; D014759:Viral Envelope Proteins; C069623:gp120 protein, Simian immunodeficiency virus; D010741:Phospholipases A; D054467:Phospholipases A2