Localization of myosin, actin, alpha-actinin, tropomyosin and vinculin in surface-activated, spreading human platelets.
We observed the localization of the contractile proteins myosin, filamentous actin, alpha-actinin, tropomyosin, and vinculin in surface-activated, spreading human platelets using a single fluorescence staining procedure and conventional fluorescence microscopy. Myosin was distributed in a speckled pattern that extended radially from the granulomere. F-actin demonstrated cable-networks. Tropomyosin and alpha-actinin occurred in a punctuate distribution, and vinculin was localized at adhesion sites. Although myosin, F-actin, alpha-actinin, tropomyosin, and vinculin were not studied in resting platelets, our data support the idea that these contractile proteins are reorganized and reassembled in activated platelets during platelet function.
Takubo
T
T
Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Osaka, Japan. m3542254@med.osaka-cu.ac.jp
Hino
M
M
Suzuki
K
K
Tatsumi
N
N
D000185:Actinin / Q000032:analysis*; D000199:Actins / Q000032:analysis*; D001792:Blood Platelets / Q000737:chemistry / Q000648:ultrastructure*; D006801:Humans; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009218:Myosins / Q000032:analysis*; D015539:Platelet Activation; D014335:Tropomyosin / Q000032:analysis*; D016596:Vinculin / Q000032:analysis*
D000199:Actins; D014335:Tropomyosin; D000185:Actinin; D016596:Vinculin; D009218:Myosins