High-level expression of human interleukin-17 in the yeast Pichia pastoris.
Human interleukin-17 (hIL-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by RT-PCR, then highly expressed in the yeast Pichia pastoris in the form of the glycosylated monomer. The monomer of rhIL-17 stimulated mouse fibroblast 3T3 cells to secrete IL-6 and was specifically bound to its receptors on 3T3 cells.
Zhou
L
L
Department of Molecular Genetics, Institute of Basic Medical Sciences, Beijing, People's Republic of China.
Wang
J
J
Peng
S
S
Duan
J
J
Cai
X
X
Zou
M
M
Su
Q
Q
D016475:3T3 Cells; D000818:Animals; D003001:Cloning, Molecular; D017931:DNA Primers; D006801:Humans; D020381:Interleukin-17 / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D015850:Interleukin-6 / Q000096:biosynthesis; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D010843:Pichia; D016133:Polymerase Chain Reaction; D018123:Receptors, Interleukin / Q000378:metabolism; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology
D017931:DNA Primers; C109985:IL17RA protein, human; C491673:Il17ra protein, mouse; D020381:Interleukin-17; D015850:Interleukin-6; D018123:Receptors, Interleukin; D053722:Receptors, Interleukin-17; D011994:Recombinant Proteins