title: A molecular mechanism for electrical tuning of cochlear hair cells.
abstract: Cochlear frequency selectivity in lower vertebrates arises in part from electrical tuning intrinsic to the sensory hair cells. The resonant frequency is determined largely by the gating kinetics of calcium-activated potassium (BK) channels encoded by the slo gene. Alternative splicing of slo from chick cochlea generated kinetically distinct BK channels. Combination with accessory beta subunits slowed the gating kinetics of alpha splice variants but preserved relative differences between them. In situ hybridization showed that the beta subunit is preferentially expressed by low-frequency (apical) hair cells in the avian cochlea. Interaction of beta with alpha splice variants could provide the kinetic range needed for electrical tuning of cochlear hair cells.
authors.affiliation: Center for Hearing Sciences, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: K
authors.initials: K
authors.lastname: Ramanathan
authors.forename: T H
authors.initials: TH
authors.lastname: Michael
authors.forename: G J
authors.initials: GJ
authors.lastname: Jiang
authors.forename: H
authors.initials: H
authors.lastname: Hiel
authors.forename: P A
authors.initials: PA
authors.lastname: Fuchs
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004594:Electrophysiology; D015870:Gene Expression; D006198:Hair Cells, Auditory / Q000502:physiology*; D006801:Humans; D017403:In Situ Hybridization; D015640:Ion Channel Gating; D007700:Kinetics; D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D008564:Membrane Potentials; D018408:Patch-Clamp Techniques; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology*; D024681:Potassium Channels, Calcium-Activated; D011784:Quail; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection
chemical_list: D051038:Large-Conductance Calcium-Activated Potassium Channel beta Subunits; D051036:Large-Conductance Calcium-Activated Potassium Channels; D015221:Potassium Channels; D024681:Potassium Channels, Calcium-Activated; D012333:RNA, Messenger; D002118:Calcium