Identification of an HLA-C variant allele, Cw*0805, by sequencing based typing.
We have identified a variant HLA-C allele, Cw*0805, in DNA isolated from a blood sample used in the UK National External Quality Assessment Scheme for Histocompatibility and Immunogenetics. The DNA was initially typed by PCR-SSP and PCR-SBT as Cw*0802, 1602 with a single mismatch in exon 2 of one of the alleles. The two HLA-C alleles were separated by locus- and allele-specific PCRs and sequencing analysis showed that the Cw*0802 allele was variant in this sample, with a single mismatch in exon 2 at position 289.
Dunn
P P
PP
UK Transplant Support Service Authority, Gifford, Bristol. dunn@uktssa.demon.co.uk
Day
S
S
Harvey
J
J
Fuggle
S V
SV
Ross
J
J
D000483:Alleles; D001483:Base Sequence; D018076:DNA, Complementary; D014644:Genetic Variation; D015236:HLA-C Antigens / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
D018076:DNA, Complementary; D015236:HLA-C Antigens; C524551:HLA-C*08 antigen