The effect of bilirubin on lipid peroxidation and antioxidant enzymes in cumene hydroperoxide-treated erythrocytes.
Recently, it has been suggested that bilirubin may act as a potent biological chain-breaking antioxidant. To observe the effects of free bilirubin on antioxidant reactions in cumene hydroperoxide-treated erythrocytes (15 g hemoglobin/dl), we added bilirubin at four different concentrations (0.5, 1, 5, and 10 mg/dl). We measured the thiobarbituric acid-reactive substance and reduced glutathione levels, and some antioxidant enzyme activities, namely superoxide dismutase, catalase, and glucose-6-phosphate dehydrogenase. Thiobarbituric acid-reactive substance and chemiluminescent signals decreased during the incubation. Superoxide dismutase activities also decreased but not as much as in the control group. Glucose-6-phosphate dehydrogenase activities and reduced glutathione levels increased, but catalase activities remained the same as the control group. Our results suggest that bilirubin--in the concentrations we have used--partially prevented the oxidant effects of cumene hydroperoxide.
Yeşilkaya
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Akdeniz University, School of Medicine, Department of Biochemistry, Antalya, Turkey. yesilka@med.akdeniz.edu.tr
Yeğin
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Ozdem
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Aksu
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D000975:Antioxidants / Q000378:metabolism; D001555:Benzene Derivatives / Q000494:pharmacology*; D001663:Bilirubin / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008163:Luminescent Measurements; D016877:Oxidants / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D000975:Antioxidants; D001555:Benzene Derivatives; D016877:Oxidants; D017392:Thiobarbituric Acid Reactive Substances; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; C007164:cumene hydroperoxide; D001663:Bilirubin