Focal cerebral ischemia in the mouse: description of a model and effects of permanent and temporary occlusion.
Our study is to demonstrate the advantages and disadvantages of middle cerebral artery occlusion (MCAO) model in the mouse. CD-1 mice had permanent MCAO for 24 h, or temporary occlusion for either 1 h followed by 23 h of reperfusion or 2 h of occlusion with 22 h of reperfusion. The infarct volume and blood-brain barrier disruption were smaller in the 1-h/23-h temporary occlusion than in either the 24-h permanent occlusion group or the 2-h/22-h temporary occlusion group (p<0.05). Our study demonstrates that blood flow, infarct volume, and blood-brain barrier disruption remain important markers of focal cerebral ischemia.
Mao
Y
Y
Department of Neurosurgery, Institute of Neurology, Hua Shan Hospital, Shanghai Medical University, Shanghai 200040, China.
Yang
G Y
GY
Zhou
L F
LF
Stern
J D
JD
Betz
A L
AL
D000208:Acute Disease; D000818:Animals; D001157:Arterial Occlusive Diseases / Q000473:pathology*; D001812:Blood-Brain Barrier / Q000502:physiology; D002536:Cerebral Arteries / Q000473:pathology*; D002908:Chronic Disease; D004195:Disease Models, Animal; D066298:In Vitro Techniques; D002546:Ischemic Attack, Transient / Q000473:pathology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D015424:Reperfusion