Mechanism of double-strand DNA cleavage effected by iron-bleomycin.
We have observed that in the absence of hydrogen peroxide the Fe(III)-bleomycin (BLM) complex exhibits high DNA cleavage efficiency, converting supercoiled Form I DNA (pBR322 or phix174) to Form II (nicked, relaxed circular); the present study may give an important clue to elucidate the fact that iron-bleomycin mediated double-strand DNA cleavage requires at least one molecule of oxygen (O2) over the amount required to form 'activated bleomycin."
Kobayashi
T
T
Institute for Molecular Science, Myodaijimachi, Okazaki, Japan.
Guo
L L
LL
Nishida
Y
Y
D001761:Bleomycin / Q000031:analogs & derivatives* / Q000737:chemistry; D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D007202:Indicators and Reagents; D007501:Iron; D007700:Kinetics; D008958:Models, Molecular; D009532:Nickel; D009690:Nucleic Acid Conformation; D010100:Oxygen; D010957:Plasmids / Q000737:chemistry*
D007202:Indicators and Reagents; C023503:iron bleomycin; D001761:Bleomycin; D009532:Nickel; D004247:DNA; D007501:Iron; D010100:Oxygen