Phagocytosis of Escherichia coli and Staphylococcus aureus by neutrophils of human immunodeficiency virus-infected patients.
To study the influence of human immunodeficiency virus (HIV) infection on phagocytosis of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by polymorphonuclear neutrophils (PMN) from HIV-infected patients in vitro. PMN were isolated from the blood of 25 HIV-infected patients (group 1: CD subset4 </= 250/microL: 14 patients, group 2: CD subset4 >250/microL: 11 patients ) by Percoll density gradient and incubated with E. coli and S. aureus. Subculture technique was used to determine PMN function at 0, 0.5, 1, 2, 4, 6, and 24 hours. Controls were run with PMN from healthy volunteers. Phagocytosis of E.coli and S.aureus by PMN of HIV-infected patients was significantly lower in group 1 (p <0.05). Reduced phagocytosis of E. coli and S. aureus was found in HIV-infected patients with low CD subset4-cell counts. Our findings may contribute to increased susceptibility to bacterial infection in HIV-infected patients with low CD subset4-cell counts.
Schaumann
R
R
Zentrum der Inneren Medizin, Medizinische Klinik III / Infektiologie, Klinikum der Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7, D-60590 Frankfurt/Main, Germany.
Krosing
J
J
Shah
P M
PM
D017088:AIDS-Related Opportunistic Infections / Q000209:etiology / Q000276:immunology; D001424:Bacterial Infections / Q000209:etiology / Q000276:immunology; D018791:CD4 Lymphocyte Count; D016022:Case-Control Studies; D004926:Escherichia coli / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D066298:In Vitro Techniques; D009504:Neutrophils / Q000276:immunology*; D010587:Phagocytosis; D013211:Staphylococcus aureus / Q000276:immunology*