Characterization of a new HLA-B39 allele, B*3913, in a Brazilian Caucasian.
A panel of samples, previously typed by serology, was retyped using a line probe assay. One sample from a Brazilian Caucasian individual was serologically typed as B52/B39, but showed an aberrant HLA-B pattern on the diagnostic strip and was typed as B*52012/B*39new. Further analysis by allele-specific amplification and subsequent sequencing of exons 2 and 3 revealed a G(B*3908)-to-T nucleotide substitution at position 467 (codon 156) resulting in an Arg (B*3908)-to-Leu substitution. Furthermore, the sequence revealed a silent mutation at position 174 (codon 58): a G(B*3908)-to-A nucleotide switch. The sequence has been sent to the EMBL databank and the HLA Nomenclature Committee, and the allele was named B*3913.
Maertens
R
R
Innogenetics N.V., Ghent, Belgium.
De Canck
I
I
Moraes
M E
ME
Vande Casteele
N
N
Van Reybroeck
G
G
Vanderborght
B
B
Moraes
J R
JR
Rossau
R
R
D000483:Alleles; D000595:Amino Acid Sequence; D001483:Base Sequence; D001938:Brazil; D018076:DNA, Complementary; D015235:HLA-B Antigens / Q000235:genetics*; D059946:HLA-B39 Antigen; D006801:Humans; D008969:Molecular Sequence Data; D044465:White People / Q000235:genetics*
D018076:DNA, Complementary; D015235:HLA-B Antigens; D059946:HLA-B39 Antigen