title: Metabolic studies on eel (Anguilla anguilla L.) hepatocytes in primary culture: effect of 17 beta-estradiol and growth hormone.
abstract: Previous studies demonstrated that native and recombinant growth hormone from mammalian and fish species potentiate the estrogenic induction of vitellogenin synthesis by cultured eel hepatocytes. In the present study, the metabolic competence (respiratory activity and estradiol catabolism) of cultured hepatocytes and their functional capacity to synthesize a specific protein, vitellogenin, in the presence of estradiol and/or bovine growth hormone was investigated. In addition, we examined the possible role of insulin-like growth factors as mediators of growth hormone. Hepatocytes retain a high level of metabolic activity under the primary culture conditions applied. Estradiol has a half life of several hours in the hepatocyte culture, and is metabolized into conjugated forms. Estradiol and/or growth hormone had no effects on respiratory activity of the cultured hepatocytes. Moreover, the estradiol catabolic parameters were not affected by growth hormone. Finally, human and trout recombinant insulin-like growth factors do not potentiate vitellogenin synthesis induced by estradiol.
authors.affiliation: Laboratoire de Physiologie Générale et Comparée MNHN, URA 90 CNRS, Paris, France.
authors.forename: P
authors.initials: P
authors.lastname: Peyon
authors.forename: R
authors.initials: R
authors.lastname: Calvayrac
authors.forename: S
authors.initials: S
authors.lastname: Baloche
authors.forename: E
authors.initials: E
authors.lastname: Burzawa-Gérard
mesh_terms: D000811:Anguilla; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D019382:Human Growth Hormone / Q000494:pharmacology*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007335:Insulin-Like Growth Factor II / Q000378:metabolism; D008099:Liver / Q000166:cytology* / Q000201:enzymology*; D008839:Microelectrodes; D010101:Oxygen Consumption / Q000187:drug effects; D014819:Vitellogenins / Q000378:metabolism
chemical_list: D014819:Vitellogenins; D019382:Human Growth Hormone; D004958:Estradiol; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D004247:DNA; D002374:Catalase