Plasma analysis of alpha-difluoromethylornithine using pre-column derivatization with naphthalene-2,3-dicarboxaldehyde/CN and multidimensional chromatography.
A procedure for the plasma analysis of alpha-difluoromethylornithine (DFMO) has been developed that utilizes pre-column derivatization with naphthalene-2,3-dicarboxaldehyde/cyanide (NDA/CN) in pH 9.2 borate buffer. Selective derivatization of delta-amine of DFMO followed by quenching of the reaction results in the formation of a cyanobenz [f] isoindole (CBI) derivative that is stable for 24 h. Plasma was prepared for derivatization by a single step procedure which resulted in an ultrafiltrate compatible with derivatization and analysis. The DFMO derivative (CBI-DFMO) was separated from plasma interferences by multidimensional chromatography with an analysis time of 28 min. The response for DFMO in plasma was linear over the range of 2.1 x 10(-8) 2.1 x 10(-6) M after derivatization. This procedure encompasses a useful linear range and offers the advantages of minimal sample preparation and production of a stable fluorophor.
Kilkenny
M L
ML
Hoechst Marion Roussell Incorporated, Kansas, MO 64134-0627, USA.
Slavik
M
M
Riley
C M
CM
Stobaugh
J F
JF
D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000518:Eflornithine / Q000097:blood*; D005453:Fluorescence; D007202:Indicators and Reagents / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*
D007202:Indicators and Reagents; D009281:Naphthalenes; C027717:2,3-naphthalenedicarboxaldehyde; D000518:Eflornithine