Molecular characterization of a calcium-binding protein from the filarial parasite Dirofilaria immitis.
A full length D. immitis cDNA (nDiCal) encoding a protein with significant similarity to the calreticulin protein family was isolated from a 6-day fourth-stage larval cDNA expression library by immunoscreening, using serum from a rabbit immunized by repeated injection of small numbers of third-stage larvae. nDiCal is 1538 bp long and contains the 21 bp nematode splice leader sequence SL1 at the 5' end. nDiCal encodes for a protein (pDiCal) with a predicted molecular mass of 46 kDa. pDiCal sequence analysis revealed similarities with calreticulin, a protein that typically resides in the endoplasmic reticulum. pDiCal possesses three consensus sequences of the calreticulin family of proteins: a neutral N-terminal region with a putative signal sequence; a proline- and tryptophan-rich P region; and a highly acidic C-terminal region. A 45Ca2+-overlay assay showed that recombinant pDiCal (rDiCal) is a Ca2+-binding protein. Antibodies to rDiCal identified a 56 kDa native antigen in all developmental stages including the excretory-secretory products derived from larvae and adult worms. Localization studies demonstrated the ubiquitous presence of pDiCal with intense expression in the hypodermis and syncitial muscle cells in both male and female adult worms. Labeling was also seen in the developing embryos within the uterus of the female worms. Sera from immune as well as chronically-infected microfilaremic dogs contained antibodies that bind rDiCal. In addition, immunoblot analysis showed that serum from a rabbit immunized with L3 cuticles reacted with rDiCal.
Tsuji
N
N
Heska Corporation, Fort Collins, CO 80525, USA.
Morales
T H
TH
Ozols
V V
VV
Carmody
A B
AB
Chandrashekar
R
R
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth; D000918:Antibody Specificity; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D037282:Calreticulin; D009033:Culicidae / Q000469:parasitology; D018076:DNA, Complementary / Q000096:biosynthesis; D004183:Dirofilaria immitis / Q000737:chemistry* / Q000254:growth & development; D004285:Dogs; D005260:Female; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015151:Immunoblotting; D007150:Immunohistochemistry; D007814:Larva / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011817:Rabbits; D011994:Recombinant Proteins / Q000096:biosynthesis; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D000909:Antibodies, Helminth; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D018076:DNA, Complementary; C117856:DiCal protein, Dirofilaria immitis; D015801:Helminth Proteins; D011994:Recombinant Proteins; D012261:Ribonucleoproteins