Establishment of an adrenocortical carcinoma xenograft with normotensive hyperaldosteronism in vivo.
We established a xenograft line of human adrenocortical carcinoma (ADR-1), and analyzed the hyperaldosteronism induced by the xenograft in vivo. Adrenocortical carcinoma specimens from a 25-year-old woman were subcutaneously inoculated into nude mice (BALB/c-nu/nu) followed by serial passages in vivo. ADR-1 retained the histopathological features (trabecular and sinusoid nests) seen in the primary carcinoma. The patient showed hyperaldosteronism (serum aldosterone >4000 pg/ml) and hypokalemia (serum K 2.1 mEq/l), but did not show hypertension. The nude rat (F344-rnu/rnu) bearing ADR-1 showed hyperaldosteronism (serum aldosterone 3320+/-1420 pg/ml; control 191+/-130 pg/ml) and hypokalemia (serum K 3.4+/-0.4 mEq/l; control 5.2+/-1.0 mEq/l) in vivo, and hypertension was not obvious. ADR-1 was shown immunohistochemically to retain production of human-specific corticosteroid synthetase. The xenograft ADR-1 will be useful to elucidate the regulatory mechanism of normotensive hyperaldosteronism.
Yamazaki
H
H
Department of Pathology, Tokai University School of Medicine, Kanagawa, Japan.
Abe
Y
Y
Katoh
Y
Y
Sawa
N
N
Ohnishi
Y
Y
Tanaka
Y
Y
Sasano
H
H
Oshika
Y
Y
Tokunaga
T
T
Kijima
H
H
Tamaoki
N
N
Nakamura
M
M
Ueyama
Y
Y
D000310:Adrenal Gland Neoplasms / Q000473:pathology / Q000503:physiopathology*; D000328:Adult; D000818:Animals; D001794:Blood Pressure; D002277:Carcinoma / Q000473:pathology / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006929:Hyperaldosteronism / Q000503:physiopathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology / Q000503:physiopathology*; D051381:Rats; D014183:Transplantation, Heterologous