The structure of the human NDUFV1 gene encoding the 51-kDa subunit of mitochondrial complex I.
The genomic organization of the human 51-kDa subunit gene (NDUFV1) on human Chromosome (Chr) 11q13 was determined. The NDUFV1 gene consists of 10 exons. Exon 1 encodes for the 20-amino-acids-long import sequence, and exon 1 through 10 codes for the 444-amino-acids-long mature protein. The protein sequence is highly conserved between human and bovine. Northern blotting analysis showed that the NDUFV1 gene expression varies widely among tissues and that in testis a unique mRNA species is present. In comparison with the other complex I flavoproteins, the expression of the 51-kDa gene in pancreatic tissue is high.
de Coo
R F
RF
Dept. of Human Genetics, University Hospital Nijmegen, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands.
Buddiger
P A
PA
Smeets
H J
HJ
van Oost
B A
BA
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D002417:Cattle; D003001:Cloning, Molecular; D042967:Electron Transport Complex I; D005091:Exons; D015723:Gene Library; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
C112232:NDUFV1 protein, human; D011506:Proteins; D009245:NADH Dehydrogenase; D042967:Electron Transport Complex I