title: Cloning of a second nm23-M1 cDNA: expression in the central nervous system of adult mouse and comparison with nm23-M2 mRNA distribution.
abstract: Nm23 has been identified as a gene family encoding different isoforms of the nucleoside diphosphate kinase. This protein is a key enzyme in the control of cellular concentrations of nucleoside triphosphates. Moreover, it has been shown to play important roles in various cellular functions such as differentiation and metastasis. In the present study, a second cDNA for nucleoside diphosphate kinase A (Nm23-M1) was isolated from a cDNA library of mouse embryonic stem cells. This clone encodes the same putative 152 aminoacids long protein as an already published cDNA but is longer in both its 5' and 3' untranslated regions. Tissue and cellular distribution of nm23-M1 mRNA was investigated by using Northern blot analysis and in situ hybridization. Nm23-M1 transcripts were found to be widely distributed throughout the mouse central nervous system with prominent expression in several restricted areas. No differences were noticed between the distribution of long and short transcripts. Furthermore, a similar pattern of expression was described in the central nervous system for nm23-M2 mRNA, encoding a second isoform of the nucleoside diphosphate kinase. However, the transcript of this isoform displayed a wider distribution and was expressed in all organs analysed by northern blotting. The possible involvement of nm23-M1 in differentiation of mouse nervous system is further discussed.
authors.affiliation: Laboratoire de Biologie de la Différenciation et du Développement, Université Bordeaux 2, 146, rue Léo Saignat, 33 076, Bordeaux Cedex, France.sandrine.dabernat@biocell.u-bordeaux2.fr
authors.forename: S
authors.initials: S
authors.lastname: Dabernat
authors.forename: M
authors.initials: M
authors.lastname: Larou
authors.forename: K
authors.initials: K
authors.lastname: Massé
authors.forename: T
authors.initials: T
authors.lastname: Hökfelt
authors.forename: G
authors.initials: G
authors.lastname: Mayer
authors.forename: J Y
authors.initials: JY
authors.lastname: Daniel
authors.forename: M
authors.initials: M
authors.lastname: Landry
mesh_terms: D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002478:Cells, Cultured; D002490:Central Nervous System / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020559:Monomeric GTP-Binding Proteins; D005810:Multigene Family; D054778:NM23 Nucleoside Diphosphate Kinases; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009701:Nucleoside-Diphosphate Kinase / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D054778:NM23 Nucleoside Diphosphate Kinases; D009419:Nerve Tissue Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; C517939:Nme1 protein, mouse; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins