Protein expression of cancer associated genes: biopsy material compared to resection material in laryngeal cancer.
BACKGROUND
Changes in several (onco)genes and their protein expression play a role in the development of Head and Neck Squamous Cell Carcinoma (HNSCC). If protein expression is to be used for clinical purposes, their expression should preferably be evaluated during the initial diagnostic work-up when only biopsy material will be available. To investigate the correlation between assessment of expression in biopsy and resection material, protein expression in both was evaluated and compared.


METHODS
In this study we compared the expression of P53, Rb protein, E-Cadherin, Ep-CAM, Desmoplakin1 and cortactin by immunohistochemistry. Expression of the proteins was studied in biopsy and resection material of 26 laryngeal carcinomas.


RESULTS
A variable rate of mismatches between the scoring on biopsy and resection material was found for the different proteins.


CONCLUSIONS
If protein expression is to be used in clinical practice and decision making it should be realized that a discrepancy exists between the expression in biopsy material and the complete resection material.
Takes
R P
RP
Department of Otolaryngology, Leiden University Medical Center, The Netherlands.
Baatenburg de Jong
R J
RJ
Keuning
J
J
Hermans
J
J
Schuuring
E
E
Van Krieken
H J
HJ
D000951:Antigens, Neoplasm / Q000032:analysis / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D015820:Cadherins / Q000032:analysis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D015815:Cell Adhesion Molecules / Q000032:analysis / Q000235:genetics; D051356:Cortactin; D003598:Cytoskeletal Proteins / Q000032:analysis / Q000235:genetics; D051180:Desmoplakins; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000601:surgery; D008297:Male; D008840:Microfilament Proteins / Q000032:analysis / Q000235:genetics; D009367:Neoplasm Staging; D009857:Oncogenes; D016160:Retinoblastoma Protein / Q000032:analysis / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000235:genetics
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C495868:CTTN protein, human; D015820:Cadherins; D015815:Cell Adhesion Molecules; D051356:Cortactin; D003598:Cytoskeletal Proteins; D051180:Desmoplakins; D000071858:Epithelial Cell Adhesion Molecule; D008840:Microfilament Proteins; D016160:Retinoblastoma Protein; D016159:Tumor Suppressor Protein p53