Differential expression and properties of phenylalanine ammonia-lyase isoforms in tomato leaves.
In tomato leaves, excision and light treatments increase phenylalanine ammonia-lyase activity, which is contributed by three PAL isoforms. These isoforms possessed similar native and subunit Mr, but were different in their pI, Km for Phe and optimal pH for activity. Also these were differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway.
Sarma
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AD
School of Life Sciences, University of Hyderabad, India.
Sreelakshmi
Y
Y
Sharma
R
R
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D018506:Gene Expression Regulation, Plant; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D018551:Solanum lycopersicum / Q000201:enzymology*; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology
D007527:Isoenzymes; D010650:Phenylalanine Ammonia-Lyase