title: T cell engraftment in lymphoid tissues of human peripheral blood lymphocyte reconstituted SCID mice with or without prior activation of cells.
abstract: The reconstitution of severe combined immunodeficiency (SCID) mice with human PBL (Hu-PBL-SCID) was assessed using fresh unstimulated PBL and anti-CD3-stimulated PBL. Mice were reconstituted with PBL by intraperitoneal injection of 1-2.5 x 107 PBL in PBS; controls received PBS. Successful engraftment of human PBL in SCID mice was determined by measurement of human IgG in mouse sera, polymerase chain reaction (PCR) detection of human-specific HLA-DRbeta DNA in SCID periphery, and immunohistochemical staining of mouse tissues (spleen, lymph nodes, thymus, liver and lung) with antibodies specific for human CD45 and CD3. Human IgG was detected 1 week after reconstitution in sera of all animals that received at least 1 x 107 PBL and continued to increase for 8 weeks. Human-specific HLA-DRbeta DNA was detected in the majority of mice 3 weeks after reconstitution but not in controls. Moreover, immunohistochemical analysis of Hu-PBL-SCID mouse tissues revealed the presence of human CD45+ cells in all tissues examined. CD3+ T cell engraftment was observed in lymphoid tissues irrespective of whether PBL had been activated prior to transfer or not.
authors.affiliation: Renal Research Laboratory, Renal Dialysis Unit, Princess Alexandra Hospital, Woolloongabba, Brisbane, Queensland, Australia. mdcolive@dingo.uq.edu.au
authors.forename: C
authors.initials: C
authors.lastname: Olive
authors.forename: C
authors.initials: C
authors.lastname: Cheung
authors.forename: M C
authors.initials: MC
authors.lastname: Falk
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000032:analysis* / Q000276:immunology; D004247:DNA / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D017493:Leukocyte Common Antigens / Q000032:analysis; D018655:Lymphocyte Count; D008221:Lymphoid Tissue / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID / Q000276:immunology*; D009928:Organ Specificity; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology / Q000637:transplantation*
chemical_list: D017252:CD3 Complex; D006684:HLA-DR Antigens; D007074:Immunoglobulin G; D004247:DNA; D017493:Leukocyte Common Antigens