title: Urocanic acid isomers and photosensitivity in healthy children.
abstract: Episodes of intense sun exposure, particularly in childhood, seem to carry a risk for the development of malignant melanoma in later life. However, little is known about photosensitivity and natural photoprotection in children. In adult subjects, photoprotection is provided mainly by the epidermal content of melanin and the thickness of the stratum corneum, while the amount of urocanic acid (UCA), a major ultraviolet-absorbing component of the stratum corneum, is not thought to contribute significantly to photoprotection. The minimal erythema dose (MED) was determined in 22 healthy children aged 6-13 years and in 36 healthy adults (mean age 28.1 years). Pigmentation was measured at six body sites by use of reflectance spectroscopy and the concentration of UCA isomers was measured in a sun-exposed area (upper back) and in unexposed buttock skin. No significant differences between children and adults were found, either in pigmentation at exposed and unexposed body sites, or in MED. The concentration of total UCA was significantly higher in the children than in the adults on the buttock (median 22.2 vs. 13.6 nmol/cm2), but not on the back. On exposed back skin, the children had a significantly higher percentage of cis-UCA than the adults (median 60.1 vs. 28.3%), while no difference was found on the buttock. In both groups, a significant correlation was found between pigmentation and MED (children: Spearman correlation coefficient 0. 58, P = 0.006; adults: Spearman correlation coefficient 0.69, P < 0. 0001), indicating that pigmentation is of major importance in determining photosensitivity in children as well as in adults. The concentration of total UCA did not correlate with the MED in either group.
authors.affiliation: Department of Dermatology D 92, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, DK-2400 NV Copenhagen, Denmark.
authors.forename: F
authors.initials: F
authors.lastname: de Fine Olivarius
authors.forename: H C
authors.initials: HC
authors.lastname: Wulf
authors.forename: J
authors.initials: J
authors.lastname: Crosby
authors.forename: M
authors.initials: M
authors.lastname: Norval
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D003880:Dermatology / Q000379:methods; D004890:Erythema / Q000209:etiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010787:Photosensitivity Disorders / Q000175:diagnosis*; D012880:Skin Pigmentation / Q000528:radiation effects*; D013472:Sunlight / Q000009:adverse effects; D014560:Urocanic Acid / Q000032:analysis* / Q000528:radiation effects
chemical_list: D014560:Urocanic Acid