title: cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase: human complex I cDNA characterization completed.
abstract: NADH:ubiquinone oxidoreductase (complex I) is an extremely complicated multiprotein complex located in the inner mitochondrial membrane. Its main function is the transport of electrons from NADH to ubiquinone, which is accompanied by translocation of protons from the mitochondrial matrix to the intermembrane space. Human complex I appears to consist of 41 subunits of which 34 are encoded by nDNA. Here we report the cDNA sequences of the hitherto uncharacterized 8 nuclear encoded subunits, all located within the hydrophobic protein (HP) fraction of complex I. Now all currently known 41 proteins of human NADH:ubiquinone oxidoreductase have been characterized and reported in literature, which enables more complete mutational analysis studies of isolated complex I-deficient patients.
authors.affiliation: University Hospital Nijmegen, Nijmegen Center for Mitochondrial Disorders, The Netherlands.
authors.forename: J L
authors.initials: JL
authors.lastname: Loeffen
authors.forename: R H
authors.initials: RH
authors.lastname: Triepels
authors.forename: L P
authors.initials: LP
authors.lastname: van den Heuvel
authors.forename: M
authors.initials: M
authors.lastname: Schuelke
authors.forename: C A
authors.initials: CA
authors.lastname: Buskens
authors.forename: R J
authors.initials: RJ
authors.lastname: Smeets
authors.forename: J M
authors.initials: JM
authors.lastname: Trijbels
authors.forename: J A
authors.initials: JA
authors.lastname: Smeitink
mesh_terms: D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology* / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D019143:Evolution, Molecular; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics; D008969:Molecular Sequence Data; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D016660:NAD(P)H Dehydrogenase (Quinone)