Identification of domains involved in superantigenicity of streptococcal pyrogenic exotoxin F (SpeF).
A series of 11 synthetic peptides of 30 amino acids, each with 10 amino acids overlap which spanned the entire sequence of streptococcal pyrogenic exotoxin F (SpeF), were employed in proliferation studies on human peripheral blood mononuclear cells (PBMCs). Regions 41-70, 141-170 and 181-210 were identified as important for SpeF-induced lymphocyte activation. Secondary structure predictions of these peptides showed similarities to regions in other superantigens known to be important for T cell mitogenicity. Furthermore, antisera specific to peptides covering amino acids 1-70 and 181-228 were able to inhibit SpeF-induced mitogenicity by 25% when pre-incubated with SpeF prior to PBMC activation.
Eriksson
A
A
Department of Clinical Bacteriology, Umeå University, Umeå, S-901 85, Sweden.
Holm
S E
SE
Norgren
M
M
D000276:Adjuvants, Immunologic / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D001426:Bacterial Proteins; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D005098:Exotoxins / Q000737:chemistry* / Q000276:immunology; D006433:Hemocyanins / Q000737:chemistry; D008213:Lymphocyte Activation; D008565:Membrane Proteins; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis; D017433:Protein Structure, Secondary; D011749:Pyrogens / Q000737:chemistry* / Q000276:immunology; D011817:Rabbits; D013290:Streptococcal Infections / Q000276:immunology; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000276:immunology*; D018089:Superantigens / Q000737:chemistry* / Q000276:immunology
D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D005098:Exotoxins; D008565:Membrane Proteins; D010455:Peptides; D011749:Pyrogens; C117980:SpeA protein, Streptococcus pyogenes; D018089:Superantigens; C027951:erythrogenic toxin; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin