Cadmium removal from human plasma by Cibacron Blue F3GA and thionein incorporated into polymeric microspheres.
Poly(2-hydroxyethylmethacrylate-ethyleneglycoldimethacrylate) [poly(HEMA-EGDMA)] microspheres carrying Cibacron Blue F3GA and/or thionein were prepared and used for the removal of cadmium ions Cd(II) from human plasma. The poly(HEMA-EGDMA) microspheres, in the size range of 150-200 microm in diameter, were produced by a modified suspension copolymerization of HEMA and EGDMA. The reactive triazinyl dye-ligand Cibacron Blue F3GA was then covalently incorporated into the microspheres. The maximum dye incorporation was 16.5 micromol/g. Then, thionein was bound onto the Cibacron Blue F3GA-incorporated microspheres under different conditions. The maximum amount of thionein bound was 14.3 mg/g. The maximum amounts of Cd(II) ions removed from human plasma by poly(HEMA-EGDMA)-Cibacron Blue F3GA and poly(HEMA-EGDMA)-Cibacron Blue F3GA-thionein were of 17.5 mg/g and 38.0 mg/g, respectively. Cd(II) ions could be repeatedly adsorbed and desorbed with both types of microspheres without significant loss in their adsorption capacity.
Ibrahim
E H
EH
Department of Chemistry, Hacettepe University, Ankara, Turkey.
Denizli
A
A
Bektaş
S
S
Genç
O
O
Pişkin
E
E
D002104:Cadmium / Q000097:blood* / Q000302:isolation & purification; D006801:Humans; D008668:Metallothionein / Q000737:chemistry*; D008863:Microspheres; D011108:Polymers; D014227:Triazines / Q000737:chemistry*
D011108:Polymers; D014227:Triazines; C004203:cadmium-binding protein; D002104:Cadmium; C012842:Cibacron Blue F 3GA; D008668:Metallothionein