Real-time control of purified product collection during chromatography of recombinant human insulin-like growth factor-I using an on-line assay.
During preparative reversed-phase chromatography of recombinant human insulin-like growth factor-I (IGF), the separation of IGF from IGF aggregates cannot be determined using UV absorbance. An on-line reversed-phase chromatographic assay was developed that provides a quantitative measurement of IGF and IGF aggregates every 4 min, allowing real-time control of purified IGF collection. Process control using the on-line assay is a reliable and accurate method to collect purified IGF.
Fahrner
R L
RL
Department of Recovery Sciences, Genentech, Inc., South San Francisco, CA 94080, USA. fahrner.robert@gene.com
Lester
P M
PM
Blank
G S
GS
Reifsnyder
D H
DH
D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification; D013056:Spectrophotometry, Ultraviolet; D013995:Time
D011994:Recombinant Proteins; D007334:Insulin-Like Growth Factor I