title: Den1, den2 and den3, ATP-inhibited deoxyribonucleases from Dropsophila embryonic nuclei.
abstract: Three Drosophila embryonic deoxyribonucleases, designated den1, den2 and den3, are identified in nuclear extracts separated by glycerol density gradient centrifugation. Den1, removes short products from the 5'-ends of single-stranded DNA or double-stranded DNA with either blunt or 5'-recessed termini. Den2 is inactive with single-stranded DNA and acts as 3'-exonuclease with double-stranded DNA possessing either blunt or 3'-recessed termini. Den3 preferentially uses partial duplex DNA containing single-stranded gap and it catalyzes hydrolysis, in 3'-5' direction, of one of the shorter strands that flank the gap. Nucleolytic activities of den1, den2 and den3 are inhibited with ATP.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, Beograd, Yugoslavia.
authors.forename: S
authors.initials: S
authors.lastname: Kojic
authors.forename: V
authors.initials: V
authors.lastname: Todorovic
authors.forename: D
authors.initials: D
authors.lastname: Ristic
authors.forename: A
authors.initials: A
authors.lastname: Savic
authors.forename: D
authors.initials: D
authors.lastname: Stefanovic
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000201:enzymology*; D005591:Chemical Fractionation; D003851:Deoxyribonucleases / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000196:embryology*; D004591:Electrophoresis, Polyacrylamide Gel; D005990:Glycerol / Q000378:metabolism
chemical_list: D000255:Adenosine Triphosphate; D003851:Deoxyribonucleases; D005990:Glycerol