Evaluation of interferences in rate and fixed-time nephelometric assays of specific serum proteins.
We performed interference studies for IgG, IgA, IgM, haptoglobin, and alpha1-antitrypsin assayed in serum, using either fixed-time nephelometry on the BN 100 from Behring or rate nephelometry on two analyzers from Beckman Instruments. For clear serum samples, results for IgG, IgA, IgM, and haptoglobin obtained with the three nephelometers showed good agreement. Values for alpha1-antitrypsin in clear sera were lower with the BN 100 than with the Array 360 or Immage. In lipemic samples, the BN 100 gave higher values than the Array 360 or Immage for all analytes except IgG. Addition of Intralipid to serum produced atypical reactions with the BN 100 (fixed-time nephelometry) but not with the Array 360 or Immage (rate nephelometry). The interference of lipemia on the BN 100 was also seen when the Beckman antibody was used, indicating that the effect was reagent-independent. For hemolyzed samples, the BN 100 gave higher values than the Array 360 or Immage for haptoglobin but not for the other analytes. Addition of increasing amounts of a hemolysate to serum revealed a negative interference in all assay systems. This effect was more pronounced with the Beckman reagent than with the Behring reagent in all three nephelometers and was independent of the type of instrument (fixed-time vs rate nephelometry).
Bossuyt
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X
Department of Clinical Pathology, University Hospital Leuven, Kapucijnenvoer 33, B-3000 Leuven, Belgium. xavier.bossuyt@uz.kuleuven.ac.be
Blanckaert
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N
D001798:Blood Proteins / Q000032:analysis*; D002138:Calibration; D006242:Haptoglobins / Q000032:analysis; D006461:Hemolysis; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008055:Lipids / Q000097:blood; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D000515:alpha 1-Antitrypsin / Q000032:analysis
D001798:Blood Proteins; D006242:Haptoglobins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008055:Lipids; D011933:Reagent Kits, Diagnostic; D000515:alpha 1-Antitrypsin