title: Gap junction expression following UVC-induced neoplastic transformation in human hybrid cell lines.
abstract: Decreased connexin gene expression and loss of the capacity for either homologous or heterologous intercellular communication has been associated with neoplastic transformation. We tested the hypothesis that loss of gap junctional intercellular communication (GJIC) correlates with tumorigenic potential in the HeLa x skin fibroblast human hybrid cell system. Connexin gene expression, gap junction function and tumorigenicity were determined for the non-tumorigenic somatic hybrid cell line (CGL1) and a series of UVC-induced tumorigenic cell lines derived from CGL1. CGL1 and the parental skin fibroblasts express connexin43 (alpha1 gap junction gene) mRNA and protein, form gap junctional plaques and have functional gap junctions. UVC-irradiation of CGL1 cells produced a cell line (UV12) with an aggressive tumorigenic phenotype, which lost connexin43 expression as well as both homologous and heterologous GJIC and was in this respect similar to HeLa cells. However, the phenotype of UV12 cells exhibited some instability and revertants to a less aggressive tumorigenic phenotype were isolated. These cells expressed connexin43 mRNA and protein, and demonstrated homologous GJIC. Furthermore, cells reconstituted from a tumor derived from this revertant cell line retained significant connexin43 expression and homologous GJIC, although they exhibited an aggressive tumorigenic phenotype. Thus, functional homologous GJIC cannot be dissociated from tumorigenicity in this system. However, heterologous GJIC between these same UVC-induced tumorigenic cell lines and normal human skin fibroblasts was reduced, whereas the non-tumorigenic hybrid cells showed extensive heterologous GJIC. In summary, re-acquisition of connexin43 expression and homologous GJIC does not restore the non-tumorigenic phenotype in UVC-induced tumorigenic HeLa skin fibroblast human hybrid cells. However, reduction of heterologous GJIC does correlate with tumorigenicity in this cell system.
authors.affiliation: Department of Physiology, College of Medicine, University of California Irvine, 92697, USA. grehring@uci.edu
authors.forename: G R
authors.initials: GR
authors.lastname: Ehring
authors.forename: R J
authors.initials: RJ
authors.lastname: Antoniono
authors.forename: J L
authors.initials: JL
authors.lastname: Redpath
mesh_terms: D002450:Cell Communication / Q000502:physiology / Q000528:radiation effects*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects*; D018031:Connexin 43 / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005453:Fluorescence; D017629:Gap Junctions / Q000502:physiology / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D006367:HeLa Cells / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D006822:Hybrid Cells; D010641:Phenotype; D012333:RNA, Messenger / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D018031:Connexin 43; D012333:RNA, Messenger