Novel peptides binding to the Fc-portion of immunoglobulins obtained from a combinatorial phage display peptide library.
Peptides interacting with the Fc portion of human IgG (IgG Fc) were selected from a phage display decapeptide library. The library was selected five times and interacting phage peptides were eluted either with Staphylococcal protein A or at low pH. Individual peptide phage clones were found to interact more strongly with IgG Fc than did either the original library or the wild-type phage. Increasing concentrations of protein A could competitively reduce the interaction of a peptide phage clone (FARLVSSIRY) eluted with protein A to the same level as the original library. Furthermore, when immunoglobulins from chicken, donkey, human, mouse, swine, rabbit, and sheep were included, peptide phage clones FGRLVSSIRY and TWKTSRISIF interacted strongly with human IgG Fc and porcine IgG and weakly with the immunoglobulins obtained from the other species.
Krook
M
M
Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden. margareta.krook@tbiokem.lth.se
Mosbach
K
K
Ramström
O
O
D000595:Amino Acid Sequence; D000818:Animals; D001435:Bacteriophages / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002645:Chickens; D003001:Cloning, Molecular; D018397:Equidae; D006801:Humans; D007124:Immunoenzyme Techniques; D007141:Immunoglobulin Fc Fragments / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D007074:Immunoglobulin G / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D051379:Mice; D009842:Oligopeptides / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D011817:Rabbits; D012756:Sheep; D013205:Staphylococcal Protein A / Q000378:metabolism; D013552:Swine
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library; D013205:Staphylococcal Protein A