Induction of proliferation in resting B-cells by a factor released by activated mouse spleen cells.
Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
Puri
N
N
School of Life Sciences Jawaharlal Nehru University, New Delhi, India.
Saxena
R K
RK
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000502:physiology*; D001853:Bone Marrow / Q000378:metabolism; D002455:Cell Division / Q000502:physiology; D002851:Chromatography, High Pressure Liquid; D002918:Chymotrypsin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006133:Growth Substances / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008970:Molecular Weight; D011112:Polymyxin B / Q000494:pharmacology; D011489:Protein Denaturation; D013154:Spleen / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D006133:Growth Substances; D002918:Chymotrypsin; D014357:Trypsin; D011112:Polymyxin B