Nonadheshive stationary organ culture of normal human urinary bladder mucosa.
Cold cup biopsies from normal human bladder mucosa were taken at cystoscopy. They were cut into fragments of 300-450 microns and grown in suspension in microwells base coated with agar. Within the first 24 hours of culture the fragments were totally covered by epithelium. After 7 days of culture the fragments had a morphology mimicking normal urothelial mucosa with an epithelium resting on a basement membraned for six weeks in culture. The purpose of this study was to provide a model representative of normal human urothelial mucosa for further studies of various diseases affecting the urinary bladder.
Vatne
V
V
Department of Surgery, Haukeland Hospital, University of Bergen, Norway.
Fjellbirkeland
L
L
Litlekalsoey
J
J
Hoestmark
J
J
D002453:Cell Cycle; D002455:Cell Division; D002470:Cell Survival; D016193:G1 Phase; D016195:G2 Phase; D006801:Humans; D009092:Mucous Membrane / Q000166:cytology; D009924:Organ Culture Techniques / Q000379:methods; D011003:Ploidies; D016196:S Phase; D017154:Stromal Cells / Q000166:cytology; D013997:Time Factors; D001743:Urinary Bladder / Q000166:cytology*; D019459:Urothelium / Q000166:cytology*