Absence of regulation of human polymorphonuclear oxidative burst by interleukin-10, interleukin-4, interleukin-13 and transforming growth factor-beta in whole blood.
Cytokines such as IL-10, IL-4, IL-13 and TGF-beta play a major role in the regulation of immune responses and are considered as anti-inflammatory agents mainly due to their actions on monocytes. These cytokines are also known to participate in the regulation of PMN activities. However, few and contradictory results have been reported on their direct and priming effects on the PMN oxidative burst, which is essential for killing bacteria. We used a flow cytometry method to study the effects of these cytokines on the PMN oxidative burst; we also used whole blood to avoid PMN activation related to isolation procedures and in order to simulate the in vivo situation more closely. None of the cytokines tested had direct or priming effects on PMN H2O2 production. We also show for the first time that these cytokines do not modulate TNF priming of the PMN oxidative burst in response to N-formyl peptides (fMLP). These results show that the anti-bacterial activity of PMN, in terms of the PMN respiratory burst, is not down regulated by these anti-inflammatory cytokines in whole blood.
Réglier-Poupet
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Laboratoire d'Immunologie et d'Hématologie, CHU Xavier-Bichat, 46, rue Henri-Huchard, 75877 Paris Cedex 18, France. Tel: (+33) 1 40 25 85 21, Fax: (+33) 1 40 25 88 53.
Hakim
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Gougerot-Pocidalo
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MA
Elbim
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D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D001770:Blood Bactericidal Activity / Q000276:immunology; D016207:Cytokines / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood; D066298:In Vitro Techniques; D016753:Interleukin-10 / Q000097:blood / Q000494:pharmacology; D018793:Interleukin-13 / Q000097:blood / Q000494:pharmacology; D053661:Interleukin-13 Receptor alpha1 Subunit; D015847:Interleukin-4 / Q000097:blood / Q000494:pharmacology; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D018123:Receptors, Interleukin / Q000097:blood; D053660:Receptors, Interleukin-13; D016897:Respiratory Burst / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000097:blood / Q000494:pharmacology
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; C508181:IL13RA1 protein, human; D018793:Interleukin-13; D053661:Interleukin-13 Receptor alpha1 Subunit; D018123:Receptors, Interleukin; D053660:Receptors, Interleukin-13; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D015847:Interleukin-4; D009240:N-Formylmethionine Leucyl-Phenylalanine; D006861:Hydrogen Peroxide