title: Effects of adenosine and epidermal growth factor on the growth of mouse mammary epithelial cells.
abstract: The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 h, EGF (0-100 ng/ml) and/or adenosine (0-100 microM) was added. Adenosine at concentrations of 1, 10 or 100 microM increased DNA synthesis significantly, when compared to control. Addition of epidermal growth factor (EGF) (10 ng/ml) into 1 or 10 microM adenosine showed the interaction in DNA synthesis between EGF and adenosine. A similar result was observed when 100 microM adenosine added to various concentrations of EGF (0-100 ng/ml). In the second mammary gland (thoracic) organ culture studies, mammary development scores were increased by adenosine (100 microM), EGF (100 ng/ml) and adenosine plus EGF. These results indicate that the purine nucleoside adenosine stimulates mammary epithelial cell growth and interacts with EGF in DNA synthesis of mouse mammary epithelial cells.
authors.affiliation: Department of Dairy Science, College of Animal Agriculture, Kangwon National University, Chuncheon, 200-701, Korea.
authors.forename: I S
authors.initials: IS
authors.lastname: Yuh
authors.forename: B B
authors.initials: BB
authors.lastname: Jang
authors.forename: B J
authors.initials: BJ
authors.lastname: Hong
mesh_terms: D000241:Adenosine / Q000494:pharmacology*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D004261:DNA Replication / Q000187:drug effects; D004357:Drug Synergism; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects; D005260:Female; D008321:Mammary Glands, Animal / Q000166:cytology*; D051379:Mice; D008813:Mice, Inbred ICR
chemical_list: D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor; D000241:Adenosine