title: Semaphorin-neuropilin interactions underlying sympathetic axon responses to class III semaphorins.
abstract: Neuropilin-1 and neuropilin-2 show specificity in binding to different class III semaphorins, including Sema III, Sema E, and Sema IV, suggesting that the specificity of action of these semaphorins is dictated by the complement of neuropilins expressed by responsive neurons. In support of this, we show that sympathetic axons coexpress neuropilin-1 and -2, that their responses to Sema III, Sema E, and Sema IV are affected in predicted ways by antibodies to neuropilin-1, and that neuropilin-1 and -2 can form homo- and heterooligomers through an interaction involving at least partly the neuropilin MAM (meprin, A5, mu) domain. These results support the idea that in sympathetic axons, the Sema III signal is mediated predominantly by neuropilin-1 oligomers, the Sema IV signal by neuropilin-2 oligomers, and the Sema E signal by neuropilin-1 and -2, either as homo- or heterooligomers.
authors.affiliation: Department of Anatomy, Howard Hughes Medical Institute, University of California, San Francisco 94143, USA.
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: Z
authors.initials: Z
authors.lastname: He
authors.forename: A
authors.initials: A
authors.lastname: Bagri
authors.forename: M
authors.initials: M
authors.lastname: Tessier-Lavigne
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D001665:Binding Sites; D019556:COS Cells; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D006023:Glycoproteins / Q000378:metabolism*; D046911:Macromolecular Substances; D009414:Nerve Growth Factors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D039942:Neuropilin-1; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D040121:Semaphorin-3A; D015398:Signal Transduction; D017783:Superior Cervical Ganglion / Q000502:physiology*; D014162:Transfection
chemical_list: D006023:Glycoproteins; D046911:Macromolecular Substances; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins; D040121:Semaphorin-3A; D039942:Neuropilin-1