title: Cyclin E associates with BAF155 and BRG1, components of the mammalian SWI-SNF complex, and alters the ability of BRG1 to induce growth arrest.
abstract: SWI-SNF complexes have been implicated in transcriptional regulation by chromatin remodeling. We have identified an interaction between two components of the mammalian SWI-SNF complex and cyclin E, an essential cell cycle regulatory protein required for G1/S transition. BRG1 and BAF155, mammalian homologs of yeast SWI2 and SWI3, respectively, are found in cyclin E complexes and are phosphorylated by cyclin E-associated kinase activity. In this report, we show that overexpression of BRG1 causes growth arrest and induction of senescence-associated beta-galactosidase activity, which can be overcome by cyclin E. Our results suggest that cyclin E may modulate the activity of the SWI-SNF apparatus to maintain the chromatin in a transcriptionally permissive state.
authors.affiliation: Cell Signaling Department, DNAX Research Institute, Palo Alto, California 94304, USA.
authors.forename: F
authors.initials: F
authors.lastname: Shanahan
authors.forename: W
authors.initials: W
authors.lastname: Seghezzi
authors.forename: D
authors.initials: D
authors.lastname: Parry
authors.forename: D
authors.initials: D
authors.lastname: Mahony
authors.forename: E
authors.initials: E
authors.lastname: Lees
mesh_terms: D000595:Amino Acid Sequence; D002453:Cell Cycle; D002460:Cell Line; D048429:Cell Size; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism*; D004265:DNA Helicases; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D016160:Retinoblastoma Protein / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014162:Transfection
chemical_list: D019927:Cyclin E; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D016160:Retinoblastoma Protein; C084011:SMARCA2 protein, human; C101681:SMARCC1 protein, human; C072981:SWI3 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C084108:SMARCA4 protein, human; D004265:DNA Helicases