title: Lamin B is a prompt heat shock protein.
abstract: The cellular response to hyperthermia involves the increased synthesis of heat shock proteins (HSPs) within several hours after treatment. In addition, a subset of proteins has been shown to be increased immediately after heating. These "prompt" HSPs are predominantly found in the nuclear matrix-intermediate filament fraction and are not present or detectable in unheated cells. Since the nuclear matrix has been suggested to be a target for heat-induced cell killing, prompt HSPs may play a prominent role in the heat shock response. Using Western blotting and flow cytometry, we found that an increase in the synthesis of lamin B, one of the major proteins of the nuclear lamina, is induced during heating at 45.5 degrees C but not during heating at 42 degrees C. Since it is an abundant protein which is constitutively expressed in mammalian cells, lamin B appears to be a unique member of the prompt HSP family. The kinetics of induction of lamin B during 45.5 degrees C heating did not correlate with the dose-dependent reduction in cell survival. While increased levels of lamin B during 45.5 degrees C heating do not appear to confer a survival advantage directly, a possible role for lamin B in cellular recovery after heat shock cannot be discounted.
authors.affiliation: Department of Radiological Sciences, The University of Oklahoma Health Sciences Center, Oklahoma City, USA. jdynlach@iupui.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Dynlacht
authors.forename: M D
authors.initials: MD
authors.lastname: Story
authors.forename: W G
authors.initials: WG
authors.lastname: Zhu
authors.forename: J
authors.initials: J
authors.lastname: Danner
mesh_terms: D002470:Cell Survival / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006360:Heat-Shock Proteins / Q000032:analysis / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D034921:Lamin Type B; D034882:Lamins; D008545:Melanoma; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D006360:Heat-Shock Proteins; D034921:Lamin Type B; D034882:Lamins; D009687:Nuclear Proteins