In planta localization of a beta-1,4-endoglucanase secreted by Heterodera glycines.
Polyclonal sera specific to beta-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2 (endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's stylet into root cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
Wang
X
X
Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, USA.
Meyers
D
D
Yan
Y
Y
Baum
T
T
Smant
G
G
Hussey
R
R
Davis
E
E
D000818:Animals; D002480:Cellulase / Q000276:immunology / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D009348:Nematoda / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity*; D010944:Plants / Q000201:enzymology* / Q000469:parasitology*; D014018:Tissue Distribution
D002480:Cellulase