title: Role of Pasteurella multocida porin on cytokine expression and release by murine splenocytes.
abstract: The aim of this study was to verify whether Pasteurella multocida porin can affect the expression and release of IL-1alpha, IL-6, TNF-alpha, IL-4, IFN-gamma, IL-10 and IL-12 by murine splenocytes in vitro. P. multocida porin and lipopolysaccharide (LPS) were able to induce the release of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 in a dose-dependent fashion. The greatest release of these cytokines was obtained using P. multocida porin at a concentration of 5 microg ml(-1) and LPS at a concentration of 1 microg ml(-1). The time-courses of release showed that P. multocida LPS was able to stimulate the production of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 earlier than porin and at a greater rate. No effect was observed on IL-4 and IL-10 release under the same experimental conditions. P. multocida porin and LPS were also able to up-regulate the mRNA expression of IL-1alpha, IL-6, TNF-alpha, IFN-gamma and IL-12 p40. Our findings suggest that P. multocida porin is able to modulate inflammatory and immunological responses by affecting the release of several cytokines and the expression of their genes.
authors.affiliation: Dipartimento di Patologia, Profilassi ed Ispezione degli Alimemti, Sezione Malattie Infettive, Facoltà di Veterinaria, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Iovane
authors.forename: P
authors.initials: P
authors.lastname: Pagnini
authors.forename: M
authors.initials: M
authors.lastname: Galdiero
authors.forename: G
authors.initials: G
authors.lastname: Cipollaro de l'Ero
authors.forename: M
authors.initials: M
authors.lastname: Vitiello
authors.forename: M
authors.initials: M
authors.lastname: D'Isanto
authors.forename: A
authors.initials: A
authors.lastname: Marcatili
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D007375:Interleukin-1 / Q000096:biosynthesis / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016979:Pasteurella multocida; D018272:Porins / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D013154:Spleen / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000235:genetics
chemical_list: D016207:Cytokines; D007375:Interleukin-1; D015850:Interleukin-6; D008070:Lipopolysaccharides; D018272:Porins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10