Expression of epstein-barr virus nuclear antigen 1 is associated with enhanced expression of CD25 in the Hodgkin cell line L428.
Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression was associated with significantly enhanced CD25 expression (interleukin 2 [IL-2]-receptor alpha chain) in transient and stably transfected L428 cells but did not affect the expression of IL-2 receptor beta and gamma chains. There was no up-regulation of the B-cell activation molecules CD23, CD30, CD39, CD40, CD44, CD71, and CD54 (intercellular adhesion molecule 1) or enhanced production of IL-6, IL-10, lymphotoxin alpha, and the soluble form of CD25. Stable EBNA-1-expressing L428 cells were nontumorigenic in SCID mice but showed enhanced lymphoma development in nonobese diabetic-SCID mice compared to mock-transfected cells.
Kube
D
D
Klinik für Innere Medizin I, Cologne, Germany. ail14@pop.rrz.uni-koln.de
Vockerodt
M
M
Weber
O
O
Hell
K
K
Wolf
J
J
Haier
B
B
Grässer
F A
FA
Müller-Lantzsch
N
N
Kieff
E
E
Diehl
V
V
Tesch
H
H
D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015197:Carcinogenicity Tests; D016207:Cytokines / Q000378:metabolism; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D014162:Transfection; D014407:Tumor Cells, Cultured
D015703:Antigens, CD; D016207:Cytokines; D019309:Epstein-Barr Virus Nuclear Antigens; D015375:Receptors, Interleukin-2