nm23-H1 protein expression in newly diagnosed and relapsed childhood acute lymphoblastic leukemia.
In a retrospective study, immunocytochemistry was used to analyze the cells of 62 newly diagnosed and 28 relapsed childhood acute lymphoblastic leukemias (ALL) for the expression of nm23-H1 protein. Of the 62 newly diagnosed ALL, only 9 cases exhibited positive staining (15%) while 10 of the 28 relapsed ALL did (36%). This difference is statistically significant (P = 0.03, Fisher's exact test). Furthermore, the presence of mutations in the exons 1-5 was investigated by RT-PCR and single-strand conformation polymorphism (SSCP) analysis. No mutations could be detected in either the newly diagnosed or relapsed ALL. This finding suggests that nm23-H1 mutations may be a rare event in ALL.
Koomägi
R
R
German Cancer Research Center, Department of Oncological Diagnostics and Therapy, Heidelberg, Germany.
Sauerbrey
A
A
Zintl
F
F
Volm
M
M
D014408:Biomarkers, Tumor / Q000032:analysis*; D001853:Bone Marrow / Q000473:pathology; D002648:Child; D006801:Humans; D007150:Immunohistochemistry; D020559:Monomeric GTP-Binding Proteins; D009154:Mutation; D054778:NM23 Nucleoside Diphosphate Kinases; D009701:Nucleoside-Diphosphate Kinase / Q000032:analysis; D018807:Polymorphism, Single-Stranded Conformational; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000097:blood / Q000235:genetics* / Q000473:pathology*; D012008:Recurrence; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000032:analysis*
D014408:Biomarkers, Tumor; D054778:NM23 Nucleoside Diphosphate Kinases; D014157:Transcription Factors; C517938:NME1 protein, human; D009701:Nucleoside-Diphosphate Kinase; D020559:Monomeric GTP-Binding Proteins