The gene, ialA, associated with the invasion of human erythrocytes by Bartonella bacilliformis, designates a nudix hydrolase active on dinucleoside 5'-polyphosphates.
ialA, one of two genes associated with the invasion of human red blood cells by Bartonella bacilliformis, the causative agent of several diseases, has been cloned and expressed in Escherichia coli. The protein, IalA, contains an amino acid array characteristic of a family of enzymes, the Nudix hydrolases, active on a variety of nucleoside diphosphate derivatives. IalA has been purified, identified, and characterized as an enzyme catalyzing the hydrolysis of members of a class of signaling nucleotides, the dinucleoside polyphosphates, with its highest activity on adenosine 5'-tetraphospho-5'-adenosine (Ap4A), but also hydrolyzing Ap5A, Ap6A, Gp4G, and Gp5G. In each case, a pyrophosphate linkage is cleaved yielding a nucleoside triphosphate and the remaining nucleotide moiety.
Conyers
G B
GB
Department of Biophysics, The Johns Hopkins University, Baltimore, Maryland 21218, USA.
Bessman
M J
MJ
D017766:Acid Anhydride Hydrolases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins; D001473:Bartonella / Q000235:genetics* / Q000472:pathogenicity*; D003001:Cloning, Molecular; D015226:Dinucleoside Phosphates / Q000378:metabolism; D004912:Erythrocytes / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology; D005798:Genes, Bacterial; D001467:Hordeum / Q000201:enzymology; D006801:Humans; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D001426:Bacterial Proteins; D015226:Dinucleoside Phosphates; C020733:diadenosine tetraphosphate; D017766:Acid Anhydride Hydrolases; C117042:NudH protein, Bartonella bacilliformis; C109470:adenosine tetraphosphatase; C020023:bis(5'-nucleosyl)tetraphosphatase (asymmetrical)