Application of NMR spectroscopy in biochemical studies of tumor cells sensitive and resistant to anticancer drugs.
Drug resistance is a prominent problem of cancer therapy. Differences in quantity and quality of many metabolites in normal and malignant cells and their changes after treatment by anticancer drugs can be detected by nuclear magnetic resonance (NMR) both in vivo and in vitro. The results of in vivo and in vitro 1H, 13C, 19F and 31P NMR spectroscopy and their correlation with the degree of resistance to anticancer drugs are discussed. Monitoring of treatment and development of drug resistance by this non-invasive method could be useful not only in cancer research related to drug resistance but also in clinical medical oncology.
Hanusovská
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Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
Dovinová
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Tkác
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Novotný
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L
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000737:chemistry / Q000473:pathology; D002247:Carbon Isotopes; D003404:Creatinine / Q000032:analysis; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004734:Energy Metabolism; D005260:Female; D005461:Fluorine; D006801:Humans; D007554:Isotopes; D007773:Lactates / Q000032:analysis; D008055:Lipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008819:Mice, Nude; D009363:Neoplasm Proteins / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis; D010759:Phosphorus Isotopes; D014407:Tumor Cells, Cultured / Q000737:chemistry / Q000187:drug effects
D000596:Amino Acids; D000964:Antimetabolites, Antineoplastic; D000970:Antineoplastic Agents; D002247:Carbon Isotopes; D007554:Isotopes; D007773:Lactates; D008055:Lipids; D009363:Neoplasm Proteins; D010759:Phosphorus Isotopes; D010725:Phosphocreatine; D005461:Fluorine; D003404:Creatinine