title: Molecular characterization of a novel Drosophila gene which is expressed in the central nervous system.
abstract: We have isolated and characterized a novel Drosophila melanogaster gene (noe) that is specifically and abundantly expressed in the central nervous system (CNS). The gene, which maps to 74B on the left arm of third chromosome, encodes a protein of 74 amino acids with no significant similarity to known protein sequences. The deduced amino acid sequence of the gene product is rich in basic amino acids, especially the lysine, and contains five potential phosphorylation sites. The noe gene lacks introns and seems to produce two transcripts by alternative polyadenylations. The promoter region deduced from 5'-RACE analysis contains a sequence similar to the TATA-box consensus sequence. RNA blot analysis detected 1.0 kb noe transcripts that are expressed from the third-instar larval stage to the adult stage and which are predominantly found in the adult heads. In situ hybridizations to tissue sections showed that the gene is abundantly expressed in neuronal cell bodies as well as in the neurophiles of adult and larval CNS (brain, optic lobe, and thoracic ganglia of adults and larval brain).
authors.affiliation: Institute of Genetic Engineering and Natural Sciences, Department of Genetic Engineering, Kyung Hee University, Yongin City, Korea.
authors.forename: B
authors.initials: B
authors.lastname: Kim
authors.forename: R D
authors.initials: RD
authors.lastname: Shortridge
authors.forename: C
authors.initials: C
authors.lastname: Seong
authors.forename: Y
authors.initials: Y
authors.lastname: Oh
authors.forename: K
authors.initials: K
authors.lastname: Baek
authors.forename: J
authors.initials: J
authors.lastname: Yoon
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism*; D002874:Chromosome Mapping; D004247:DNA / Q000737:chemistry / Q000235:genetics; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D004268:DNA-Binding Proteins / Q000737:chemistry*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000737:chemistry / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect / Q000235:genetics*; D017403:In Situ Hybridization; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D062085:RNA, Long Noncoding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016385:TATA Box; D014018:Tissue Distribution
chemical_list: D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C117803:lncRNA:noe, Drosophila; D004247:DNA