title: Studies on pulmonary and systemic Aspergillus fumigatus-specific IgE and IgG antibodies in horses affected with chronic obstructive pulmonary disease (COPD).
abstract: Inhalant exposure to Aspergillus fumigatus (Asp. f.) antigens induces marked inflammatory and immunological alterations in the lungs of horses affected with chronic obstructive pulmonary disease (COPD). In this study we investigated the role of specific allergen(s) present in Asp. f. on systemic and pulmonary IgE and IgG responses in control and COPD-affected horses, using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting techniques. Compared with controls, horses affected with COPD had significantly higher levels of BALF IgE and IgG to somatic Asp. f. antigens as well as to the allergen 1/a (Asp. f. 1/a). Serum levels of IgE and IgG against these antigens did not differ between control and COPD-affected horses. Antigen specific IgE and IgG levels did not correlate between BALF and serum. Scanning of Asp. f. and IgE and IgG blots revealed bands that are recognised by both IgE- and IgG-specific antibodies. Additionally, all horses responded with BALF IgE- and IgG-specific for 93, 35, 31 and 23 kDa allergens, suggesting that these antigens are involved in the induction of airway IgE and IgG responses. These allergens may have the potential to be used as biomarkers for the diagnosis of Asp. f. related exacerbations of equine COPD.
authors.affiliation: Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, UK.
authors.forename: K H
authors.initials: KH
authors.lastname: Schmallenbach
authors.forename: I
authors.initials: I
authors.lastname: Rahman
authors.forename: H H
authors.initials: HH
authors.lastname: Sasse
authors.forename: P M
authors.initials: PM
authors.lastname: Dixon
authors.forename: R E
authors.initials: RE
authors.lastname: Halliwell
authors.forename: B C
authors.initials: BC
authors.lastname: McGorum
authors.forename: R
authors.initials: R
authors.lastname: Crameri
authors.forename: H R
authors.initials: HR
authors.lastname: Miller
mesh_terms: D000818:Animals; D001228:Aspergillosis / Q000276:immunology / Q000662:veterinary*; D001232:Aspergillus fumigatus / Q000276:immunology*; D001992:Bronchoalveolar Lavage Fluid; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology*; D008172:Lung Diseases, Fungal / Q000276:immunology / Q000662:veterinary*; D008173:Lung Diseases, Obstructive / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D008297:Male
chemical_list: D007074:Immunoglobulin G; D007073:Immunoglobulin E