Induction of steroid 21-hydroxylase/beta-galactosidase transgene expression by unilateral adrenalectomy.
Unilateral adrenalectomy was used to induce compensatory growth in the contralateral adrenal gland of transgenic mice bearing a steroid 21-hydroxylase promoter/beta-galactosidase reporter (21-OHase/beta-gal) transgene, in which 6.4 kb of 5'-flanking sequence of the mouse steroid 21-OHase A gene are linked to a LacZ reporter gene. 48 hours following removal of the right adrenal gland, the left gland of transgene-positive mice showed a 4.5 fold increase in specific activity of the beta-gal reporter, compared to the right gland, while left glands from sham-operated transgene-positive and unilateral adrenalectomized transgene-negative mice showed no such increase. The increased specific transgene reporter activity, relative to total adrenal gland protein, must result from up-regulation of transgene expression, rather than from the compensatory increase in adrenocortical mass. This suggests that elements regulating trophic hormone-mediated 21-OHase gene expression in vivo are located within 6.4 kb of the 21-OHase gene transcription start site.
Lindsay
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A
Department of Clinical Biochemistry, University of Edinburgh, RIE, UK.
Sharples
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A
Morley
S D
SD
D000315:Adrenalectomy / Q000379:methods*; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D017930:Genes, Reporter / Q000502:physiology; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics; D013255:Steroid 21-Hydroxylase / Q000235:genetics*; D013997:Time Factors; D019076:Transgenes / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics*
D013255:Steroid 21-Hydroxylase; D001616:beta-Galactosidase