PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.
We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.
Metherell
L A
LA
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
Logan
J M
JM
Stanley
J
J
D015373:Bacterial Typing Techniques; D002167:Campylobacter / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D002169:Campylobacter Infections / Q000175:diagnosis / Q000382:microbiology*; D015342:DNA Probes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D013045:Species Specificity
D015342:DNA Probes; D012336:RNA, Ribosomal, 16S