Evolution of bamboo mosaic virus in a nonsystemic host results in mutations in the helicase-like domain that cause reduced RNA accumulation.
A mutant population of bamboo mosaic potexvirus (BaMV) was isolated after serial passage using Chenopodium quinoa plants. While the wild type inoculum induced indistinct chlorotic lesions, the mutant produced obvious lesions on C. quinoa although RNA accumulation of the mutant in Nicotiana benthamiana protoplasts was significantly reduced compared to wild type. Mutations were identified in the helicase-like domain. One RT-PCR-generated cDNA clone (designated pL1-33) representing the helicase-like region showed four nucleotide mutations encoding three amino acid changes that were shown to result in dramatically decreased viral accumulation. Independent analyses of the effects of these substitutions showed that nucleotide changes at position 1722 resulting in a leucine to proline switch and position 2129 resulting in a histidine to tyrosine switch had the greatest effect on viral accumulation. Combination of these two mutations resulted in a undetectable viral accumulation. We have identified that amino acids within the helicase domain but outside the universally conserved helicase-like motifs that play an important role in viral amplification.
Huang
C Y
CY
Graduate Institute of Agricultural Biotechnology, National Chung Hsing University, Taichung, Taiwan.
Tsai
C H
CH
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000378:metabolism; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D016366:Open Reading Frames; D006109:Poaceae / Q000821:virology; D017863:Potexvirus / Q000201:enzymology / Q000235:genetics*; D020365:RNA Helicases / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism* / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
D000596:Amino Acids; D012367:RNA, Viral; D020365:RNA Helicases