Comparison of the phagocytosis of two types of cyclosporin (SDZ OXL 400 and SDZ IMM 125) by alveolar macrophages from hamsters.
The aim of this study was to compare two types of cyclosporin (Cs) particles, SDZ OXL 400 and SDZ IMM 125, the latter being more hydrophilic, to understand their uptake by airway macrophages. Alveolar macrophages (AM), harvested by bronchoalveolar lavage (BAL) of hamster lungs, were cultured with two different doses (0.1 mg and 0.5 mg) for 1 h, 6 h, and 24 h. Control incubations without Cs particles or with latex particles were carried out simultaneously. Cell viability, cell activation (i.e., respiratory burst, interleukin-6 (IL-6) synthesis) and mean volume of particles phagocytosed per macrophage were measured. Both types of Cs particles did not modify the AM viability, and failed to induce IL-6 synthesis during phagocytosis but slightly decreased the cell oxidative respiratory burst. The comparison between SDZ OXL 400 and SDZ IMM 125 showed that for the lower dose the mean volume of both Cs types phagocytosed was similar at 1 h and 6 h. At 24 h an increase of the mean volume phagocytosed was seen for SDZ IMM 125 but not for SDZ OXL 400. For the higher dose the mean volume of SDZ IMM 125 phagocytosed was higher than SDZ OXL 400 at 1 h and 6 h and comparable for both types at 24 h. SDZ IMM 125 particles were phagocytosed more rapidly than SDZ OXL 400. The mean volume of phagocytosed latex particles increased with time and dose and was higher than for both Cs particle types. In conclusion, AM were seen to phagocytose particles of different physical properties (i.e., form, size, and shape), chemical properties (i.e., inert or peptidic) and degrees of hydrophilicity in a different manner.
Maye
I
I
Institute of Anatomy, University of Bern, Switzerland.
de Fraissinette
A
A
Cruz-Orive
L M
LM
Andersen
E
E
Niederlander
C
C
Bueb
W
W
Froehlich
C
C
Schreier
M
M
Vonderscher
J
J
Richter
F
F
Cordier
A
A
Gehr
P
P
D000818:Animals; D002470:Cell Survival; D006224:Cricetinae; D003524:Cyclosporins / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D007166:Immunosuppressive Agents / Q000493:pharmacokinetics*; D015850:Interleukin-6 / Q000096:biosynthesis; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008647:Mesocricetus; D010587:Phagocytosis
D003524:Cyclosporins; D007166:Immunosuppressive Agents; D015850:Interleukin-6; C117614:SDZ OXL 400; C075935:SDZ IMM 125