[The isolation and analysis of the highly repetitive DNA from the argali].
The repeated DNA sequence of wild ram (Ovis ammon) of 800 bp has been cloned. The blot-hybridization, in situ-hybridization, sequencing and computer analysis were used for the sequence analysis. It was shown that the cloned DNA is from 1.714 gm/cm3 repeated satellite DNA family. Fourteen highly homologous sequences were revealed in the nucleotide sequence databases. An analysis of their alignment revealed presence of two subfamilies (A and B). Average divergence of subfamily A. sequences (including the wild ram repeated sequence) from consensus is about 1%.
Fedorova
E V
EV
Rogozin
I B
IB
Ptitsyn
A A
AA
Cheriaukene
O V
OV
Kaftanovskaia
E M
EM
D000818:Animals; D000835:Animals, Wild; D001483:Base Sequence; D002478:Cells, Cultured; D016384:Consensus Sequence; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004276:DNA, Satellite / Q000235:genetics; D006041:Goats / Q000235:genetics; D008297:Male; D008969:Molecular Sequence Data; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods; D012689:Sequence Homology, Nucleic Acid; D012756:Sheep / Q000235:genetics*
D004276:DNA, Satellite; D004247:DNA