title: Green fluorescent protein purification by organic extraction.
abstract: Green fluorescent protein (GFP) is widely used as an excellent reporter molecule in biochemistry and cell biology. Some biochemical and immunological assays require high-purity GFP. However, the majority of current procedures for GFP purification include multiple time-consuming chromatography steps with a low yield of the desired product or require tag-containing proteins. An alternative method is described for the GFP purification without affinity extensions using organic extraction yielding a highly homogeneous protein indistinguishable in spectroscopic properties from that purified by previous methods.
authors.affiliation: Branch of the Institute of Bioorganic Chemistry RAS, Pushchino, Moscow Region, 142292, Russia. yak@fibkh.serpukhov.su
authors.forename: A V
authors.initials: AV
authors.lastname: Yakhnin
authors.forename: L M
authors.initials: LM
authors.lastname: Vinokurov
authors.forename: A K
authors.initials: AK
authors.lastname: Surin
authors.forename: Y B
authors.initials: YB
authors.lastname: Alakhov
mesh_terms: D020001:1-Butanol; D000645:Ammonium Sulfate; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002725:Chloroform; D002853:Chromatography, Liquid; D002942:Circular Dichroism; D004926:Escherichia coli / Q000235:genetics; D000431:Ethanol; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000302:isolation & purification*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives; D012997:Solvents; D013050:Spectrometry, Fluorescence; D014867:Water
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D012997:Solvents; D014867:Water; D049452:Green Fluorescent Proteins; D000431:Ethanol; C020927:phenyl-sepharose; D002725:Chloroform; D020001:1-Butanol; D012685:Sepharose; D000645:Ammonium Sulfate