title: Effect of postischaemic hypothermia on the mitochondrial damage induced by ischaemia and reperfusion in the gerbil.
abstract: In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37 degreesC or 30 degreesC. After normothermic (37 degreesC) ischaemia/reperfusion, significant decreases in mitochondrial state 3 (+ADP) oxygen consumption (-42.2%), complex II-III activity in synaptosomes (-31.7%) and complex IV were measured, in both free mitochondria and synaptosomes (-30.3% and -27. 8% respectively). However, following hypothermic (30 degreesC) reperfusion, both respiration rates and all enzyme activities remained at levels not significantly different from those in the sham operated controls.
authors.affiliation: Department of Neurochemistry, Institute of Neurology, Queen Square, London WC1N 3BG, UK.l.canevari@ion.ucl.ac.uk
authors.forename: L
authors.initials: L
authors.lastname: Canevari
authors.forename: A
authors.initials: A
authors.lastname: Console
authors.forename: E A
authors.initials: EA
authors.lastname: Tendi
authors.forename: J B
authors.initials: JB
authors.lastname: Clark
authors.forename: T E
authors.initials: TE
authors.lastname: Bates
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000628:therapy*; D005849:Gerbillinae; D007036:Hypothermia, Induced; D008928:Mitochondria / Q000473:pathology*; D010101:Oxygen Consumption / Q000502:physiology; D015427:Reperfusion Injury / Q000473:pathology*; D013574:Synaptosomes / Q000473:pathology