Topology of the thyroid transcription factor 1 homeodomain-DNA complex.
The topology of the thyroid transcription factor 1 homeodomain (TTF-1HD)-DNA complex was investigated by a strategy which combines limited proteolysis and selective chemical modification experiments with mass spectrometry methodologies. When limited proteolysis digestions were carried out with the protein in the absence or presence of its target oligonucleotide, differential peptide maps were obtained from which the amino acid residues involved in the interaction could be inferred. Similarly, selective acetylation of lysine residues in both the isolated and the complexed homeodomain allowed us to identify the amino acids protected by the interaction with DNA. Surface topology analysis of isolated TTF-1HD performed at neutral pH was in good agreement with the three-dimensional structure of the molecule as determined by NMR studies under acidic conditions. Minor differences were detected in the C-terminal region of the protein which, contrary to NMR data, showed no accessibility to proteases. Analysis of the complex provided an experimental validation of the model proposed on the basis of the homology with the homeodomain structures described so far. An increased accessibility of the C-terminal region was observed following the interaction, suggesting its displacement from the protein core by the oligonucleotide molecule. Comparative experiments with DNA fragments differing in sequence and binding capabilities highlighted structural differences among the complexes, mainly located in the N-terminal region of the homeodomain, thus accounting for their different dissociation constants.
Scaloni
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A
Centro Internazionale di Servizi di Spettrometria di Massa, CNR-Università di Napoli "Federico II", Italy.
Monti
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M
Acquaviva
R
R
Tell
G
G
Damante
G
G
Formisano
S
S
Pucci
P
P
D000107:Acetylation; D000818:Animals; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D018398:Homeodomain Proteins / Q000737:chemistry* / Q000378:metabolism; D006868:Hydrolysis; D008239:Lysine / Q000378:metabolism; D046911:Macromolecular Substances; D013058:Mass Spectrometry; D008958:Models, Molecular; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D009841:Oligonucleotides / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D013961:Thyroid Gland / Q000737:chemistry*; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism
D018398:Homeodomain Proteins; D046911:Macromolecular Substances; C000614416:Nkx2-1 protein, rat; D009687:Nuclear Proteins; D009841:Oligonucleotides; D000074482:Thyroid Nuclear Factor 1; D014157:Transcription Factors; D004247:DNA; D010450:Endopeptidases; D008239:Lysine