title: Preparation of a monoclonal antibody specific for human stanniocalcin.
abstract: Stanniocalcin (STC) is a glycoprotein hormone that was first identified in fish, where it regulates the calcium level in the body fluid. The cDNA which encodes human STC has recently been reported but the function has not been completely elucidated. We have prepared a monoclonal antibody against human STC using an analogous peptide of the putative antigenic domain in human STC; it was conjugated with keyhole limpet hemocyanin (KLH). The monoclonal antibody specifically stained the distal convoluted tubules in human kidney which is a putative target organ of STC. The ELISA was established using the monoclonal antibody and recombinant human STC as a standard antigen. The monoclonal antibody prepared in this study provides a useful tool for clinical studies of STC in human.
authors.affiliation: Department of Clinical and Laboratory Medicine, Tohoku University, School of Medicine, Sendai, Miyagi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Koide
authors.forename: A
authors.initials: A
authors.lastname: Igarashi
authors.forename: N
authors.initials: N
authors.lastname: Sakuma
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: S
authors.initials: S
authors.lastname: Shibata
authors.forename: K
authors.initials: K
authors.lastname: Tada
authors.forename: S
authors.initials: S
authors.lastname: Takano
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000595:Amino Acid Sequence; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D000918:Antibody Specificity; D006023:Glycoproteins / Q000276:immunology*; D006728:Hormones / Q000276:immunology*; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology; D017386:Sequence Homology, Amino Acid
chemical_list: D000911:Antibodies, Monoclonal; D006023:Glycoproteins; D006728:Hormones; D010455:Peptides; C051795:teleocalcin