High-density screen of human tumor cell lines for homozygous deletions of loci on chromosome arm 8p.
Tumor cell-specific homozygous deletions coinciding at a particular genetic location may indicate the inactivation of a nearby tumor suppressor gene. Forty-six human cancer cell lines of prostate, pancreatic, lung, liver, and colon origin were screened for homozygous deletions of 139 expressed sequence tag (EST) and sequence-tagged site (STS) loci spanning the entire short arm of chromosome 8. Only one Southern blot-verified homozygous deletion was detected in this set of cell lines. The deletion, in pancreatic tumor cell line MIA-PaCa-2, encompassed two screening loci, D8S549 and D8S1992, and overlapped another previously described homozygous deletion of band 8p22 in a metastatic prostate cancer specimen. Both deletions entirely removed the candidate tumor suppressor gene N33. These data define a consensus homozygous deletion region in chromosome band 8p22.
Levy
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A
Gene Discovery Lab, Canji, Inc., San Diego, California 92121, USA.
Dang
U C
UC
Bookstein
R
R
D002872:Chromosome Deletion; D002874:Chromosome Mapping / Q000379:methods; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005819:Genetic Markers; D006720:Homozygote; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D014407:Tumor Cells, Cultured
D004273:DNA, Neoplasm; D005819:Genetic Markers