Study of the effect of polybutylcyanoacrylate nanoparticles and their metabolites on the primary immune response in mice to sheep red blood cells.
Polybutylcyanoacrylate nanoparticles (PBCN) and their metabolites (polycyanoacrylic acid--PCAA, and n-butanol) were compared with respect to their effects on the primary immune response of mice to sheep red blood cells (SRBC). PCAA was synthetized via a Knoevenagel reaction. Antibody production (hemagglutinins) and E-rosette-forming cells (E-RFC) were used to document the induction of antigen-specific immune response to SRBC in all immunized mice. PBCN showed a time- and dose-dependent effect on the immune response, both humoral and cellular. The inductive phase of immune response was affected preferably. The high dose of PBCN (200 mg kg(-1)) tended to suppress the immune response. This was expressed more in mice treated before antigenic stimulation. Lower dose (10 mgkg(-1)) stimulated the immune response. A significant difference was found in the effects of PBCN and their metabolites on the immune response. PCAA and n-butanol administered at doses equivalent (after lysosomal hydrolysis) to the doses applied of intact PBCN did not impair significantly the immune response. A clear time dependence and dose dependence were not observed. The study led to the hypothesis that the greater suppressive effect of PBCN, relative to either PCAA or n-butanol, or a mixture of them, is probably due to the blocking of the immunopresenting function of macrophages instead of some toxicity towards the immunocompetent cells.
Simeonova
M
M
Scientific Research Centre for Speciality Polymers, Sofia, Bulgaria.
Chorbadjiev
K
K
Antcheva
M
M
D000818:Animals; D000910:Antibodies, Heterophile / Q000096:biosynthesis; D001789:Blood Group Antigens / Q000276:immunology; D000440:Butanols / Q000378:metabolism / Q000494:pharmacology; D004659:Enbucrilate / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010316:Particle Size; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology
D000910:Antibodies, Heterophile; D001789:Blood Group Antigens; D000440:Butanols; D004659:Enbucrilate