Performance characteristics of an enzyme-linked immunosorbent assay for determining salivary immunoglobulin G response to Helicobacter pylori.
We evaluated the salivary immunoglobulin G (IgG) immune response to Helicobacter pylori in 70 subjects by enzyme-linked immunosorbent assay (ELISA). Subjects with a positive H. pylori culture showed significantly higher titers of antibodies than subjects with no detectable H. pylori: the overall sensitivity and specificity of the test were 84 and 90%, respectively. The detection of salivary anti-H. pylori IgG antibodies may be considered as an alternative to serum IgG detection for ease of sample collection or when blood samples are not available in screening of patients with dyspepsia.
De Pascalis
R
R
Cattedra di Microbiologia Clinica, Dipartimento di Biologia e Patologia Cellulare e Molecolare, Universita' degli Studi di Napoli "Federico II," 80131 Naples, Italy. pascalir@mail.nih.gov
Del Pezzo
M
M
Nardone
G
G
Budillon
G
G
Lavitola
A
A
D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D003470:Culture Media; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000276:immunology; D016480:Helicobacter pylori / Q000276:immunology* / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000097:blood; D012463:Saliva / Q000276:immunology* / Q000382:microbiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D003470:Culture Media; D007074:Immunoglobulin G