title: Transcriptional interferences between normal or mutant androgen receptors and the activator protein 1--dissection of the androgen receptor functional domains.
abstract: We investigated the interferences of the normal or mutated androgen receptor with the activator protein-1 (AP-1) by assessing their effects on transcriptional activity in CV-1 cells. A luciferase reporter gene was constructed downstream from either a promoter for the mouse vas deferens protein, or a trimerized 12-O-tetradecanoyl phorbol-13-acetate-response element site whose transcriptions are activated by androgen and 12-O-tetradecanoyl phorbol-13-acetate, a potent AP-1 activator. The blockade of dephosphorylation by protein phosphatases identifies the protein phosphatases that modulate the AP-1/androgen receptor cross-talk. Using engineered or naturally occurring androgen receptor mutants that are responsible for complete or partial androgen insensitivity syndromes, we defined the subregions involved in the cross-talk of the androgen receptor with the AP-1 factors. First, it appears that the 188 first amino acids of the N-terminal domain of the androgen receptor are necessary to obtain a full transrepression. Second, a functional and intact ligand binding domain is critical for the modulation of androgen/AP-1 pathway interactions. Third, normal DNA binding capacity of the androgen receptor is not required. Two mutants at positions 568 and 581 of the DNA binding domain demonstrate that the transactivation and transrepression functions of the androgen receptor can be dissociated. Collectively, these data indicate that several segments of the androgen receptor are involved in cross-talk with the AP-1 pathway. Mutations within the DNA binding domain of the androgen receptor highly impair these interferences.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, INSERM U439, Pathologie Moléculaire des Récepteurs Nucléaires, Montpellier, France.
authors.forename: J M
authors.initials: JM
authors.lastname: Lobaccaro
authors.forename: N
authors.initials: N
authors.lastname: Poujol
authors.forename: B
authors.initials: B
authors.lastname: Térouanne
authors.forename: V
authors.initials: V
authors.lastname: Georget
authors.forename: S
authors.initials: S
authors.lastname: Fabre
authors.forename: S
authors.initials: S
authors.lastname: Lumbroso
authors.forename: C
authors.initials: C
authors.lastname: Sultan
mesh_terms: D000449:Aldehyde Reductase; D000728:Androgens / Q000378:metabolism; D000818:Animals; D018567:Breast Neoplasms, Male / Q000378:metabolism; D019556:COS Cells; D004247:DNA / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D017930:Genes, Reporter; D005818:Genetic Engineering; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008297:Male; D008387:Marine Toxins; D051379:Mice; D009154:Mutation; D019319:Okadaic Acid / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D011944:Receptors, Androgen / Q000737:chemistry* / Q000235:genetics*; D019311:Staurosporine / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection
chemical_list: D000728:Androgens; D004791:Enzyme Inhibitors; D008387:Marine Toxins; D010080:Oxazoles; D011506:Proteins; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D011944:Receptors, Androgen; D018808:Transcription Factor AP-1; D019319:Okadaic Acid; C059041:calyculin A; D004247:DNA; C066498:Akr1b7 protein, mouse; D000449:Aldehyde Reductase; D008156:Luciferases; D019311:Staurosporine; D013755:Tetradecanoylphorbol Acetate