[Isolation and long-term cultivation of rabbit embryonic stem cells].
A cell line similar to embryonic stem cells has been isolated from 5-day rabbit embryos. After the disruption of zona pellucida, embryos were mechanically separated into cel aggregates, which were then cultivated on a feeder layer of primary mouse embryonic fibroblasts. Cells similar to embryonic stem cells had high nuclear-to-cytoplasm ratio. It has been demonstrated for the first time that the inner cell mass and embryonic stem cells of rabbits expressed the activity of alkaline phosphatase. Between 10 and 30% of cells retained this expression and pluripotent characteristics up to the 23rd transfer. After 14-17 transfers the obtained embryonic cells could be induced to differentiation in vitro, which resulted in the formation of embryoid bodies. After the 23rd transfer, the line of rabbit embryonic cells was lost as a result of its differentiation.
Vasil'eva
S G
SG
Federal Research Institute of Animal Husbandry, Russian Academy of Agricultural Sciences, Dubrovitsy, Moscow oblast', Russia.
Prelle
K
K
Muller
S
S
Besenfel'der
U
U
Müller
M
M
Brém
G
G
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000201:enzymology; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002469:Cell Separation; D051379:Mice; D008845:Microinjections; D011817:Rabbits; D013234:Stem Cells / Q000166:cytology* / Q000201:enzymology
D000469:Alkaline Phosphatase