The interchain disulfide linkage of T-cell antigen receptor-alpha and -beta chains is a prerequisite for T-cell activation.
Complementary DNAs encoding the T-cell antigen receptor (TCR)-alpha and mutant TCR-beta chains, lacking the interchain disulfide bond-related cysteine, were introduced into a TCR-alpha and -beta protein-deficient T-cell line. TCR-alpha and the mutant TCR-beta chains assembled with the CD3-epsilon, -gamma, -delta, and -zeta subunits and were efficiently transported to the cell surface; however, the hybrid TCR molecules exhibited a diminished response to T-cell activation by major histocompatibility complex-bound antigen, superantigen, and TCR cross-linking. These results suggest that the interchain disulfide bond between the TCR clonotypic chains is not required for TCR assembly and cell surface expression, but it plays an important role in maintaining the functional integrity of the TCR complex.
Li
Z
Z
Department of Rheumatology, Royal North Shore Hospital, New South Wales, 2065, Australia.
Wu
W
W
Kemp
O
O
Stephen
M
M
Manolios
N
N
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D004220:Disulfides / Q000378:metabolism*; D015870:Gene Expression; D008213:Lymphocyte Activation; D051379:Mice; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014162:Transfection
D004220:Disulfides; D016693:Receptors, Antigen, T-Cell, alpha-beta