title: Expression of epstein-barr virus nuclear antigen 1 is associated with enhanced expression of CD25 in the Hodgkin cell line L428.
abstract: Epstein-Barr virus is associated with several human malignancies including Burkitt's lymphoma, nasopharyngeal carcinoma, and Hodgkin's disease (HD). To examine the effect of Epstein-Barr virus nuclear antigen 1 (EBNA-1) in the pathogenesis of HD, we transfected the gene into the HD cell line L428. EBNA-1 expression was associated with significantly enhanced CD25 expression (interleukin 2 [IL-2]-receptor alpha chain) in transient and stably transfected L428 cells but did not affect the expression of IL-2 receptor beta and gamma chains. There was no up-regulation of the B-cell activation molecules CD23, CD30, CD39, CD40, CD44, CD71, and CD54 (intercellular adhesion molecule 1) or enhanced production of IL-6, IL-10, lymphotoxin alpha, and the soluble form of CD25. Stable EBNA-1-expressing L428 cells were nontumorigenic in SCID mice but showed enhanced lymphoma development in nonobese diabetic-SCID mice compared to mock-transfected cells.
authors.affiliation: Klinik für Innere Medizin I, Cologne, Germany. ail14@pop.rrz.uni-koln.de
authors.forename: D
authors.initials: D
authors.lastname: Kube
authors.forename: M
authors.initials: M
authors.lastname: Vockerodt
authors.forename: O
authors.initials: O
authors.lastname: Weber
authors.forename: K
authors.initials: K
authors.lastname: Hell
authors.forename: J
authors.initials: J
authors.lastname: Wolf
authors.forename: B
authors.initials: B
authors.lastname: Haier
authors.forename: F A
authors.initials: FA
authors.lastname: Grässer
authors.forename: N
authors.initials: N
authors.lastname: Müller-Lantzsch
authors.forename: E
authors.initials: E
authors.lastname: Kieff
authors.forename: V
authors.initials: V
authors.lastname: Diehl
authors.forename: H
authors.initials: H
authors.lastname: Tesch
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000032:analysis; D015197:Carcinogenicity Tests; D016207:Cytokines / Q000378:metabolism; D019309:Epstein-Barr Virus Nuclear Antigens / Q000096:biosynthesis* / Q000235:genetics; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000378:metabolism*; D006689:Hodgkin Disease / Q000276:immunology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: D015703:Antigens, CD; D016207:Cytokines; D019309:Epstein-Barr Virus Nuclear Antigens; D015375:Receptors, Interleukin-2