Paramagnetic effects on nuclear relaxation in enzyme-bound Co(II)-adenine nucleotide complexes: relative contributions of dipolar and scalar interactions.
31P NMR measurements on CoADP bound to creatine kinase designed to estimate the relative contribution of scalar and dipolar interactions to 31P spin relaxation rates show that these rates are primarily due to distance-dependent dipolar interactions and that the contribution of the scalar interaction is negligible.
Ray
B D
BD
Department of Physics, Indiana University-Purdue University at Indianapolis, Indianapolis, Indiana, 46202, USA.
Jarori
G K
GK
Nageswara Rao
B D
BD
D000227:Adenine Nucleotides / Q000737:chemistry*; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives* / Q000737:chemistry; D016880:Anisotropy; D001665:Binding Sites; D002412:Cations / Q000032:analysis; D003402:Creatine Kinase / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008280:Magnetics; D008961:Models, Structural; D015394:Molecular Structure; D010759:Phosphorus Isotopes; D013113:Spin Labels
D000227:Adenine Nucleotides; D002412:Cations; D010759:Phosphorus Isotopes; D013113:Spin Labels; D000244:Adenosine Diphosphate; C040338:cobalt adenosine diphosphate complex; D003402:Creatine Kinase