title: [Study of the interaction of MTPN11 mycobacteriophage with host cells based on electron microscopy, fluorimetry, and electro-oriented spectroscopy].
abstract: According to electron-microscopic data, various cells in the M. smegmatis ATCC607 population interact differently with phage MTPH11. Fluorometric studies of phage-host interactions were performed using a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfonate (ANS). Changes in the electric characteristics of mycobacterial cells infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spectroscopy for rapid phage typing of mycobacteria is discussed.
authors.affiliation: State Research Center for Applied Microbiology, Obolensk, Russia.
authors.forename: E L
authors.initials: EL
authors.lastname: Zhilenkov
authors.forename: I G
authors.initials: IG
authors.lastname: Shemiakin
authors.forename: V M
authors.initials: VM
authors.lastname: Fomchenkov
authors.forename: A Iu
authors.initials: AIu
authors.lastname: Ivanov
authors.forename: A V
authors.initials: AV
authors.lastname: Gavriushkin
authors.forename: M V
authors.initials: MV
authors.lastname: Oborotov
mesh_terms: D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D005470:Fluorometry; D008854:Microscopy, Electron; D009160:Mycobacteriophages / Q000502:physiology* / Q000648:ultrastructure; D019907:Mycobacterium fortuitum / Q000821:virology; D020102:Mycobacterium smegmatis / Q000821:virology; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate