Adrenal steroid-secreting cells express endothelin-1.
Endothelin-1 (ET-1) is involved in adrenal steroid secretion but its cell origin remains unclear. We showed, using RT-PCR the expression of the mRNAs for preproET-1 and ECE-1 in primary cultures of human adrenal cells enriched in glomerulosa cells. Since these expressions could be due to contamination of steroid secreting cells by other cells, we also used the human adrenocortical cell line H295R, which was shown to produce steroids. This cell line also expressed preproET-1-RNA and released mature ET. Functional ET receptors were shown on H295R and cultured human adrenocortical cells. These findings indicate that adrenal steroid-secreting cells synthesize and release ET-1, raising the possibility for an autocrine-paracrine effect of ET-1 on adrenocortical functions.
Mathieu
M N
MN
INSERM U. 469, CCIPE, Montpellier, France.
Hamroun
D
D
Pouzeratte
Y
Y
Guérineau
N C
NC
Guillon
G
G
Chevillard
C
C
D000302:Adrenal Cortex / Q000166:cytology* / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D016282:Aspartic Acid Endopeptidases / Q000235:genetics; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D018920:Coculture Techniques; D003470:Culture Media / Q000378:metabolism; D019332:Endothelin-1 / Q000378:metabolism* / Q000494:pharmacology; D000072396:Endothelin-Converting Enzymes; D016232:Endothelins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007295:Inositol Phosphates / Q000378:metabolism; D007425:Intracellular Membranes / Q000378:metabolism; D008666:Metalloendopeptidases; D009994:Osmolar Concentration; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D015384:Zona Glomerulosa / Q000166:cytology / Q000378:metabolism
D000305:Adrenal Cortex Hormones; D003470:Culture Media; D019332:Endothelin-1; D016232:Endothelins; D007295:Inositol Phosphates; D011498:Protein Precursors; D012333:RNA, Messenger; D016282:Aspartic Acid Endopeptidases; D008666:Metalloendopeptidases; C000606923:ECE1 protein, human; D000072396:Endothelin-Converting Enzymes; D002118:Calcium