title: Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants.
abstract: Many virus and transposon DNAs can integrate into the host genome. In this review, techniques, including inverse polymerase chain reaction (IPCR), novel Alu-PCR and vectorette- or splinkerette-PCR are introduced as possible strategies for cloning flanking DNA regions of the integrants. Targeted gene-walking PCR, restriction-site PCR, capture PCR, and panhandle PCR and boomerang DNA amplification are also described. The principles, advantages and limitations of each approach are discussed.
authors.affiliation: Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University, Taipei, Taiwan.
authors.forename: E K
authors.initials: EK
authors.lastname: Hui
authors.forename: P C
authors.initials: PC
authors.lastname: Wang
authors.forename: S J
authors.initials: SJ
authors.lastname: Lo
mesh_terms: D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral