title: Effect of fish oil supplementation on aortic compliance in rats: role of the endothelium.
abstract: Arterial compliance improves with dietary fish oils in patients with high cardiovascular risk. Since fish oils alter prostaglandin metabolism and the L-arginine-nitric oxide pathway, and since compliance may be modified by vasoactive substances, the effect of the endothelium and some of its derivatives on aortic complaince were examined. Rats were randomly allocated to four groups, the first of which fed only the regular chow. The remaining three groups were fed the chow supplemented by daily gavage with either coconut, fish or safflower oil for 8 weeks. The thoracic aorta was removed and six 2 mm rings obtained. Rings were paired and one from each pair treated with either N(W)-nitro-L-arginine, indomethacin or de-endothelialized. A diameter-tension curve was initiated from wire touch position using incremental increases in wire distance until no further response observed. The data was transformed to a diameter-pressure relationship and fitted with a linear equation, the slope of which related directly to compliance. De-endothelialization (slopes: control vs de-endothelialized: 9.05+/-0.15 vs 8.31+/-0.24; P< 0.05) and indomethacin (slopes: control vs indomethacin: 9.11+/-0.15 vs 7.76+/-0.37; P< 0.05) significantly decreased arterial compliance as did dietary fish oils (slopes: control vs n-3: 9.16+/-0.11 vs 7.84+/-0.39; P< 0.05). No further effect was seen with indomethacin in the fish oil treated group. It is concluded that the endothelium and in particular, endothelium derived prostanoids, contribute to vessel compliance. We also conclude that fish oils have a similar action to indomethacin, leading to the increase in aortic stiffness observed.
authors.affiliation: Alfred and Baker Medical Unit, Baker Medical Research Institute, Prahran, Victoria, Australia. jaye.chin@baker.edu.au
authors.forename: J P
authors.initials: JP
authors.lastname: Chin-Dusting
authors.forename: V
authors.initials: V
authors.lastname: Jovanovska
authors.forename: B A
authors.initials: BA
authors.lastname: Kingwell
authors.forename: X J
authors.initials: XJ
authors.lastname: Du
authors.forename: A M
authors.initials: AM
authors.lastname: Dart
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003187:Compliance / Q000187:drug effects; D004042:Dietary Fats, Unsaturated / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects*; D005395:Fish Oils / Q000737:chemistry / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D019335:Nitroarginine / Q000494:pharmacology; D010938:Plant Oils / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004042:Dietary Fats, Unsaturated; D005395:Fish Oils; D010938:Plant Oils; D019335:Nitroarginine; D007213:Indomethacin