Class I MHC molecule-mediated inhibition of Sindbis virus replication.
The threshold for systemic viral infection relies on the amplification of virus at a primary infection site. We have identified that class I MHC molecules can trigger the inhibition of replication of Sindbis virus in a haplotype- and allele-specific manner. Class I MHC molecules of H-2d haplotypes exhibit a strong inhibitory effect whereas H-2k haplotypes show minimal inhibition of Sindbis viral replication. By a single gene transfection of H-2d class I MHC molecules, into cells that express class I MHC molecules of H-2k haplotype and are susceptible to viral replication, these cells became resistant to viral replication. The inhibition of viral replication by class I MHC molecules occurs neither during the stage of virus entry/endocytosis nor during virus maturation. Rather, viral-specific RNA replication, as well as viral gene expression, are inhibited in cells expressing inhibitory class I MHC molecules. This class I MHC molecule-mediated inhibition requires newly synthesized host gene products, implying the activation of an intracellular signaling mechanism that is triggered by specific class I MHC molecules.
Hahn
Y S
YS
Beirne Carter Center for Immunology Research, Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908, USA.
Guanzon
A
A
Rice
C M
CM
Hahn
C S
CS
D000818:Animals; D000998:Antiviral Agents / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D001287:Attachment Sites, Microbiological / Q000276:immunology; D002460:Cell Line; D006224:Cricetinae; D005786:Gene Expression Regulation / Q000276:immunology; D006183:H-2 Antigens / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D006239:Haplotypes; D051379:Mice; D008807:Mice, Inbred BALB C; D012845:Sindbis Virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D014407:Tumor Cells, Cultured; D014779:Virus Replication / Q000276:immunology*
D000998:Antiviral Agents; D006183:H-2 Antigens