title: High prevalence of silent celiac disease in preschool children screened with IgA/IgG antiendomysium antibodies.
abstract: BACKGROUND
  Because of the different sensitivity and specificity of serologic tests, the search for silent celiac disease is usually performed with the combined or sequential use of several tests. Among these, the IgA-class endomysium antibody test has the highest specificity and positive predictive value, but it may overlook IgA-deficient patients.
  
  
  METHODS
  To test a new one-step screening approach, serum samples from 427 apparently healthy, 3- to 6-year-old Hungarian children were investigated for IgA-class and IgG-class endomysium antibodies using monkey esophagus and human jejunum as substrates.
  
  
  RESULTS
  Five new cases with flat mucosa were identified by strong endomysium antibody positivity and subsequent jejunal biopsy, yielding a celiac disease prevalence of 1:85. An additional child may have latent celiac disease (slight histologic changes at present). Two of the screening-detected celiac patients exhibited only IgG-class endomysium antibodies due to associated IgA-deficiency. Despite the young age of the screened population, antigliadin antibodies were positive in only three of the five celiac patients.
  
  
  CONCLUSIONS
  Prevalence of celiac disease in the study population was much higher than expected on the basis of antigliadin antibody-based studies. The screening system used detected celiac cases in which there was IgA-deficiency and those in which there was not and also those negative for antigliadin antibodies. The findings suggest the importance of the primary testing of autoantibodies in future celiac disease screening policies.
authors.affiliation: Department of Gastroenterology-Nephrology, Heim Pál Children's Hospital, Budapest, Hungary.
authors.forename: I R
authors.initials: IR
authors.lastname: Korponay-Szabó
authors.forename: J B
authors.initials: JB
authors.lastname: Kovács
authors.forename: A
authors.initials: A
authors.lastname: Czinner
authors.forename: G
authors.initials: G
authors.lastname: Gorácz
authors.forename: A
authors.initials: A
authors.lastname: Vámos
authors.forename: T
authors.initials: T
authors.lastname: Szabó
mesh_terms: D001323:Autoantibodies / Q000097:blood*; D002446:Celiac Disease / Q000175:diagnosis* / Q000453:epidemiology* / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003598:Cytoskeletal Proteins / Q000276:immunology; D005260:Female; D005903:Gliadin / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D008403:Mass Screening; D015995:Prevalence; D016036:Seroepidemiologic Studies; D012698:Serologic Tests
chemical_list: D001323:Autoantibodies; D003598:Cytoskeletal Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D005903:Gliadin