Peroxynitrite production and activation of poly (adenosine diphosphate-ribose) synthetase in spinal cord injury.
Peroxynitrite formation and the subsequent activation of the nuclear enzyme, poly (adenosine diphosphate [ADP]-ribose) synthetase (PARS), has been implicated in the pathogenesis of several neurodegenerative disorders. Here, we demonstrate that nitrotyrosine, an indicator of peroxynitrite generation, and poly (ADP) ribose, a marker of PARS activation, were selectively localized within tissues from spinal cord-injured rats. Our data implicate a role for peroxynitrite production and PARS activation in the development of spinal cord trauma.
Scott
G S
GS
Division of Critical Care Medicine, Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Jakeman
L B
LB
Stokes
B T
BT
Szabó
C
C
D000244:Adenosine Diphosphate / Q000096:biosynthesis*; D000818:Animals; D003288:Contusions / Q000150:complications; D005260:Female; D007796:Laminectomy; D009566:Nitrates / Q000032:analysis / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000737:chemistry / Q000201:enzymology; D013119:Spinal Cord Injuries / Q000201:enzymology* / Q000209:etiology / Q000601:surgery; D014443:Tyrosine / Q000032:analysis
D009566:Nitrates; C039642:peroxynitric acid; D014443:Tyrosine; D000244:Adenosine Diphosphate; D011065:Poly(ADP-ribose) Polymerases