title: Occurrence of cello-oligosaccharides in the apoplast of auxin-treated pea stems
abstract: Treatment of pea (Pisum sativum L.) hypocotyl segments with indole-3-butyric acid, which promotes segment elongation, increased the solubilization of both xyloglucan and cello-oligosaccharides in the apoplast of auxin-treated pea stems. The cello-oligosaccharides were isolated from the apoplastic solution with a charcoal/Celite column and were identified as cellobiose, cellotriose, and cellotetraose after subsequent thin-layer chromatography and paper electrophoresis. Cello-oligosaccharides in the apoplastic fraction were monitored using cellobiose dehydrogenase. Both xyloglucan and cello-oligosaccharides appeared to be formed concurrently within 30 min after treatment with the auxin, and the cello-oligosaccharides increased with stem elongation even after 2 h. The total activity of cellulase did not increase for up to 4 h.
authors.affiliation: Wood Research Institute, Kyoto University, Gokasho Uji, Kyoto 611, Japan (R.T., F.S., T.H.).
authors.initials: R
authors.lastname: Tominaga
authors.initials: M
authors.lastname: Samejima
authors.initials: F
authors.lastname: Sakai
authors.initials: T
authors.lastname: Hayashi