title: Effect of selenium and vitamin E supplementation on lipid abnormalities in plasma, aorta, and adipose tissue of Zucker rats.
abstract: Twenty-nine obese female Zucker rats (fa/fa) were fed with a laboratory chow supplemented or not with a selenium-rich yeast (Selenion), or Selenion + vitamin E, or vitamin E alone. Twelve lean female Zucker rats (Fa/Fa) of the same littermates fed with the same diet were used as control. After 32 wk of diet, obesity induced a large increase in plasma insulin and lipid levels. A significant decrease in the plasma vitamin E/triglycerides ratio (p<0.005) and an increase in plasma thiobarbituric reactive substances (TBARS) (p<0.005) were also observed. Plasma selenium and vitamin E increased in all supplemented rats. The plasma insulin level was decreased by selenion supplementation and the vitamin E/triglycerides ratio was completely corrected by double supplementation with Selenion + vitamin E. TBARS were also efficiently decreased in two obese groups receiving vitamin E. In plasma, adipose tissue and aorta, obesity induced an increase in palmitic acid (C16:0), a very large increase in monounsaturated fatty acids (palmitoleic acid C16:1, stearic acid C18:1) associated with a decrease in polyunsaturated n-6 fatty acids (linoleic acid C18:2 n-6, arachidonic C20:4 n-6). These alterations in fatty acid distribution were only partly modulated by Se and vitamin E supplements. However, in the aorta, antioxidant treatment in obese rats significantly reduced the increase in C16:0 and C16:1 (p<0.05 and p<0.01, respectively) and the decrease in arachidonic acid (p<0.05). These changes could be beneficial in the reduction of insulin resistance and help to protect the vascular endothelium.
authors.affiliation: National Institute of Health and Medical Research, Unit 331, Bron, France.
authors.forename: C
authors.initials: C
authors.lastname: Douillet
authors.forename: M
authors.initials: M
authors.lastname: Bost
authors.forename: M
authors.initials: M
authors.lastname: Accominotti
authors.forename: F
authors.initials: F
authors.lastname: Borson-Chazot
authors.forename: M
authors.initials: M
authors.lastname: Ciavatti
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001011:Aorta / Q000737:chemistry / Q000187:drug effects*; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight / Q000187:drug effects; D002784:Cholesterol / Q000097:blood; D019587:Dietary Supplements; D005227:Fatty Acids / Q000032:analysis / Q000097:blood / Q000737:chemistry; D005260:Female; D007333:Insulin Resistance; D008055:Lipids / Q000032:analysis* / Q000097:blood; D009765:Obesity / Q000378:metabolism; D051381:Rats; D011924:Rats, Zucker; D012643:Selenium / Q000097:blood / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014810:Vitamin E / Q000097:blood / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D005227:Fatty Acids; D008055:Lipids; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D002784:Cholesterol; D012643:Selenium