title: Neovascularization of the RPE: temporal differences in mice with rod photoreceptor gene defects.
abstract: Neovascularization (NV) of the retinal pigment epitheium (RPE) by retinal capillaries following degeneration and loss of photoreceptor cells is a widely recognized phenomenon in rodents. NV of the RPE usually occurs several weeks to months after the loss of photoreceptor cells. We have observed that NV of the RPE occurs much earlier in a line of P23H mutant rhodopsin transgenic mice than in most other mice and rats that have been previously examined. To compare the temporal course of RPE NV in P23H mice with that of two other retinal degeneration mutants with the same time course of photoreceptor cell loss, we have quantified the number of retinal capillaries in the RPE of P23H and Q344ter mutant rhodopsin transgenic mice and retinal degeneration (rd/rd) mice at ages ranging from postnatal day (P) 20-400. Retinal capillary profiles located within the RPE were already present as early as P20 in the P23H retinas, and although these usually were located where most photoreceptor nuclei were missing, they occasionally were found where 1-2 rows of photoreceptor nuclei were still present. The maximal incidence was found in P23H retinas at P100. By contrast, NV of the RPE in rd/rd and Q344ter mice occurred much later. In rd/rd, a significant number of capillary profiles was not seen in the RPE until about P130, and not until about P180 in Q344ter. Both showed maximal incidence at about P240. In all three mutants, an apparent regression of the capillaries occurred following the peak, with that in the P23H mice preceeding the other two mutants. The findings suggest that the P23H mutant rhodopsin transgenic mouse may be a useful model for studying the regulation of NV in the outer retina.
authors.affiliation: Beckman Vision Center, University of California San Francisco, San Francisco, CA, 94143-0730, USA.
authors.forename: S
authors.initials: S
authors.lastname: Nishikawa
authors.forename: M M
authors.initials: MM
authors.lastname: LaVail
mesh_terms: D000375:Aging / Q000473:pathology; D000818:Animals; D002196:Capillaries / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology; D010857:Pigment Epithelium of Eye / Q000098:blood supply*; D015861:Retinal Neovascularization / Q000235:genetics* / Q000473:pathology; D012243:Rhodopsin / Q000235:genetics*
chemical_list: D012243:Rhodopsin