title: In vivo migration and function of transferred HIV-1-specific cytotoxic T cells.
abstract: The persistence of HIV replication in infected individuals may reflect an inadequate host HIV-specific CD8+ cytotoxic T lymphocyte (CTL) response. The functional activity of HIV-specific CTLs and the ability of these effector cells to migrate in vivo to sites of infection was directly assessed by expanding autologous HIV-1 Gag-specific CD8+ CTL clones in vitro and adoptively transferring these CTLs to HIV-infected individuals. The transferred CTLs retained lytic function in vivo, accumulated adjacent to HIV-infected cells in lymph nodes and transiently reduced the levels of circulating productively infected CD4+ T cells. These results provide direct evidence that HIV-specific CTLs target sites of HIV replication and mediate antiviral activity, and indicate that the development of immunotherapeutic approaches to sustain a strong CTL response to HIV may be a useful adjunct to treatment of HIV infection.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle 98101, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Brodie
authors.forename: D A
authors.initials: DA
authors.lastname: Lewinsohn
authors.forename: B K
authors.initials: BK
authors.lastname: Patterson
authors.forename: D
authors.initials: D
authors.lastname: Jiyamapa
authors.forename: J
authors.initials: J
authors.lastname: Krieger
authors.forename: L
authors.initials: L
authors.lastname: Corey
authors.forename: P D
authors.initials: PD
authors.lastname: Greenberg
authors.forename: S R
authors.initials: SR
authors.lastname: Riddell
mesh_terms: D019264:Adoptive Transfer; D000595:Amino Acid Sequence; D002465:Cell Movement; D006679:HIV Seropositivity / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D008198:Lymph Nodes; D008969:Molecular Sequence Data; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000502:physiology