title: High-performance liquid chromatographic methods for the determination of a new carbapenem antibiotic, L-749,345, in human plasma and urine.
abstract: A column-switching, reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of a new carbapenem antibiotic assay using ultraviolet detection has been developed for a new carbapenem antibiotic L-749,345 in human plasma and urine. A plasma sample is centrifuged and then injected onto an extraction column using 25 mM phosphate buffer, pH 6.5. After 3 min, using a column-switching valve, the analyte is back-flushed with 10.5% methanol-phosphate buffer for 3 min onto a Hypersil 5 microm C18 BDS 100x4.6 mm analytical column and then detected by absorbance at 300 nm. The sample preparation and HPLC conditions for the urine assay are similar, except for a longer analytical column 150x4.6 mm. The plasma assay is specific and linear from 0.125 to 50 microg/ml; the urine assay is linear from 1.25 to 100 microg/ml.
authors.affiliation: Merck Research Laboratories, West Point, PA 19486, USA.
authors.forename: D G
authors.initials: DG
authors.lastname: Musson
authors.forename: K L
authors.initials: KL
authors.lastname: Birk
authors.forename: A M
authors.initials: AM
authors.lastname: Cairns
authors.forename: A K
authors.initials: AK
authors.lastname: Majumdar
authors.forename: J D
authors.initials: JD
authors.lastname: Rogers
mesh_terms: D000890:Anti-Infective Agents / Q000097:blood* / Q000652:urine*; D002138:Calibration; D015780:Carbapenems / Q000097:blood* / Q000652:urine*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D015780:Carbapenems; C108047:L 749345