Comparison of randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis for typing of Moraxella catarrhalis strains.
Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typing of M. catarrhalis.
Vu-Thien
H
H
Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, 75571 Paris Cedex 12, France. hoang.vuthien@trs.ap-hop-paris.fr
Dulot
C
C
Moissenet
D
D
Fauroux
B
B
Garbarg-Chenon
A
A
D000293:Adolescent; D015373:Bacterial Typing Techniques; D002648:Child; D002675:Child, Preschool; D004200:Diseases in Twins; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D007223:Infant; D001936:Moraxella catarrhalis / Q000145:classification* / Q000302:isolation & purification; D016870:Neisseriaceae Infections / Q000382:microbiology*; D019105:Random Amplified Polymorphic DNA Technique