Adiabatic mixing in the liquid state.
Adiabatic spin inversion has been used in the liquid state very efficiently for decoupling purposes. Here we show that it can also be adapted for spin mixing experiments, such as the TOCSY and clean TOCSY experiment, and is superior to previously employed mixing sequences. The main advantage of adiabatic mixing sequences over the conventional mixing schemes used in liquid state experiments is an extremely low sensitivity to RF field inhomogeneity and miscalibration of the B1 field strength. The method is evaluated experimentally by comparing results obtained with different mixing schemes in the basic 2D TOCSY experiment. In addition to higher reliability, adiabatic mixing provides a sensitivity improvement of ca. 20% as compared to conventional mixing schemes. This is explained by higher signal losses due to RF inhomogeneity in the experiments employing traditional mixing schemes. More significant sensitivity improvements can be expected in situations where RF homogeneity is traditionally poor, for example, in large volume probes and magnetic resonance imaging experiments.
Kupce
E
E
Varian NMR Instruments, Walton-on-Thames, Surrey, KT12 2QF, England, United Kingdom.
Schmidt
P
P
Rance
M
M
Wagner
G
G
D000465:Algorithms; D002138:Calibration; D006859:Hydrogen; D007089:Image Enhancement; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013997:Time Factors
D012996:Solutions; D006859:Hydrogen