Applicability of SSCP analysis for MHC genotyping: fingerprinting of Ovar-DRB1 exon 2 alleles from Finnish and Russian breeds.
The applicability of single strand conformation polymorphism (SSCP) analysis for major histocompatibility complex (MHC) genotyping in sheep was studied. A panel of Ovar-DRB1 exon 2 'allele fingerprints' was defined. The panel could accelerate DRB1 genotyping of new breeds when already existing sequences are used as references in SSCP analysis. In this study, seven new exon 2 sequences and 19 different alleles in total were detected from 31 animals of Finnish and Russian sheep breeds. Ovar-DRB1*0201 was detected in all the six grey Finnsheep animals included in this study, suggesting reduced MHC diversity within these animals.
Kostia
S
S
Department of Biosciences, University of Helsinki, Finland.
Kantanen
J
J
Kolkkala
M
M
Varvio
S L
SL
D000483:Alleles; D000818:Animals; D016172:DNA Fingerprinting / Q000662:veterinary; D005091:Exons; D014644:Genetic Variation; D005838:Genotype; D006649:Histocompatibility Antigens / Q000235:genetics*; D008285:Major Histocompatibility Complex / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational; D012756:Sheep / Q000235:genetics*
D006649:Histocompatibility Antigens