Genetic mapping of a major locus controlling pyrethroid resistance in Tribolium castaneum (Coleoptera: Tenebrionidae).
Tribolium castaneum (Herbst) strain QTC279 is highly resistant to deltamethrin and other synthetic pyrethroids. This strain was shown to carry at least 1 resistance gene, PyR-1, on linkage group 9, approximately 20 map units from the visible mutant marker, pearl. Three-point mapping involving pearl and another visible mutant marker, cola, indicated a gene order of pearl-cola-PyR-1. Evidence of a 2nd LG9-linked resistance factor (R) mapping in the gene order R-p-co was also observed. Other resistance factors were clearly present in QTC279, but were not genetically mapped. Piperonyl butoxide, an inhibitor of cytochrome P450-mediated oxidative metabolism, significantly increased the toxicity of deltamethrin to a strain derived from QTC279 that carries PyR-1, strain pR. Compared to susceptible beetles, QTC279 and pR had elevated and comparable levels of cytochrome P450 protein. The significance of pyrethroid resistance in T. castaneum is discussed.
Stuart
J J
JJ
Department of Entomology, Purdue University, West Lafayette, IN 47907-1158, USA.
Ray
S
S
Harrington
B J
BJ
Neal
J J
JJ
Beeman
R W
RW
D000818:Animals; D002874:Chromosome Mapping; D017344:Genes, Insect; D007305:Insecticide Resistance / Q000235:genetics; D011722:Pyrethrins; D014231:Tribolium / Q000235:genetics*
D011722:Pyrethrins