title: Targeted disruption of the mouse phospholipase C beta3 gene results in early embryonic lethality.
abstract: In order to investigate the biological function of phosphatidylinositol-specific phospholipase C (PLC) we generated mutant mice by gene targeting. Homozygous inactivation of PLCbeta3 is lethal at embryonic day 2.5. These mutants show poor embryonic organization as well as reduced numbers of cells. Identical phenotypes were recorded in homozygous mutants generated from two independently targeted embryonic stem cell clones. Heterozygous mutant mice, however, are viable and fertile for at least two generations. We also showed that mouse PLCbeta3 is expressed in unfertilized eggs, 3-cell and egg cylinder stages of embryos. In conclusion, these results indicate that PLCbeta3 expression is essential for early mouse embryonic development.
authors.affiliation: Department of Internal Medicine, University Hospital, Uppsala, Sweden.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.forename: S
authors.initials: S
authors.lastname: Gebre-Medhin
authors.forename: C
authors.initials: C
authors.lastname: Betsholtz
authors.forename: P
authors.initials: P
authors.lastname: Stålberg
authors.forename: Y
authors.initials: Y
authors.lastname: Zhou
authors.forename: C
authors.initials: C
authors.lastname: Larsson
authors.forename: G
authors.initials: G
authors.lastname: Weber
authors.forename: R
authors.initials: R
authors.lastname: Feinstein
authors.forename: K
authors.initials: K
authors.lastname: Oberg
authors.forename: A
authors.initials: A
authors.lastname: Gobl
authors.forename: B
authors.initials: B
authors.lastname: Skogseid
mesh_terms: D000818:Animals; D002460:Cell Line; D005260:Female; D005313:Fetal Death / Q000235:genetics*; D018390:Gene Targeting; D005804:Genes, Lethal; D006579:Heterozygote; D006801:Humans; D007150:Immunohistochemistry; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D008297:Male; D051379:Mice; D008817:Mice, Mutant Strains; D010641:Phenotype; D054799:Phospholipase C beta; D010738:Type C Phospholipases / Q000235:genetics* / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010738:Type C Phospholipases; C518039:PLCB3 protein, human; D054799:Phospholipase C beta; C518040:Plcb3 protein, mouse