title: Flow cytometric analysis of Thy-1 expression in myelodysplastic syndrome.
abstract: We analyzed the expression of Thy-1 (CD90) antigen on CD34+ bone marrow mononuclear cells (BMMNC) obtained from 25 patients with myelodysplastic syndrome (MDS) by two-color flow cytometry. Five of nine patients (55.6%) with refractory anemia with excess of blasts (RAEB) and two of 16 (12.5%) with RAEB in transformation (RAEB-t) showed more than 20% of Thy-1 expression of their CD34+ BMMNC. Regarding chromosomal abnormalities, -5/5q- or -18 might be correlated with the expression of Thy-1 on CD34+ BMMNC in MDS. Nine patients were analyzed twice, once before and once after leukemic transformation and showed no significant change in Thy-1 expression. These results show that Thy-1 was expressed on CD34+ BMMNC in certain patients with MDS before leukemic transformation and that it was maintained during the disease progression. In contrast, expression of Thy-1 did not seem essential to the leukemic transformation in MDS though the patients with high Thy-1 expression might have poorer prognosis compared with those with low Thy-1 expression.
authors.affiliation: Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan. inaba178@koto.kpu-m.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Inaba
authors.forename: C
authors.initials: C
authors.lastname: Shimazaki
authors.forename: T
authors.initials: T
authors.lastname: Sumikuma
authors.forename: K
authors.initials: K
authors.lastname: Shimura
authors.forename: R
authors.initials: R
authors.lastname: Takahashi
authors.forename: H
authors.initials: H
authors.lastname: Hirai
authors.forename: E
authors.initials: E
authors.lastname: Ashihara
authors.forename: Y
authors.initials: Y
authors.lastname: Sudo
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: H
authors.initials: H
authors.lastname: Haruyama
authors.forename: N
authors.initials: N
authors.lastname: Fujita
authors.forename: M
authors.initials: M
authors.lastname: Yoshimura
authors.forename: M
authors.initials: M
authors.lastname: Nakagawa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000754:Anemia, Refractory, with Excess of Blasts / Q000378:metabolism / Q000473:pathology; D018952:Antigens, CD34 / Q000032:analysis / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002869:Chromosome Aberrations / Q000473:pathology; D025063:Chromosome Disorders; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D018800:Thy-1 Antigens / Q000096:biosynthesis*
chemical_list: D018952:Antigens, CD34; D018800:Thy-1 Antigens