title: Coxsackievirus and adenovirus receptor amino-terminal immunoglobulin V-related domain binds adenovirus type 2 and fiber knob from adenovirus type 12.
abstract: The extracellular region of the coxsackievirus and adenovirus receptor (CAR) is predicted to consist of two immunoglobulin (Ig)-related structural domains. We expressed the isolated CAR amino-terminal domain (D1) and a CAR fragment containing both extracellular Ig domains (D1/D2) in Escherichia coli. Both D1 and D1/D2 formed complexes in vitro with the recombinant knob domain of adenovirus type 12 (Ad12) fiber, and D1 inhibited adenovirus type 2 (Ad2) infection of HeLa cells. These results indicate that the adenovirus-binding activity of CAR is localized in the amino-terminal IgV-related domain and confirm our earlier observation that Ad2 and Ad12 bind to the same cellular receptor. Preliminary crystallization studies suggest that complexes of Ad12 knob bound to D1 will be suitable for structure determination.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA. freimuth@bnl.gov
authors.forename: P
authors.initials: P
authors.lastname: Freimuth
authors.forename: K
authors.initials: K
authors.lastname: Springer
authors.forename: C
authors.initials: C
authors.lastname: Berard
authors.forename: J
authors.initials: J
authors.lastname: Hainfeld
authors.forename: M
authors.initials: M
authors.lastname: Bewley
authors.forename: J
authors.initials: J
authors.lastname: Flanagan
mesh_terms: D000260:Adenoviruses, Human / Q000378:metabolism*; D001665:Binding Sites; D002213:Capsid / Q000378:metabolism*; D036022:Capsid Proteins; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D029822:Enterovirus B, Human / Q000378:metabolism*; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D011991:Receptors, Virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: C568771:CLMP protein, human; D036022:Capsid Proteins; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D018985:Epitopes, B-Lymphocyte; D007135:Immunoglobulin Variable Region; D011991:Receptors, Virus; C018315:hexon capsid protein, Adenovirus