A transferrin D variant (DHR5) identical with DSaga by polyacrylamide gel isoelectric focusing was found in a disputed paternity case.
A transferrin (TF) variant was found in the child in a paternity case by polyacrylamide gel isoelectric focusing (PAGIF). Further analysis of serum TF disclosed that this rare TF variant was inherited from the alleged father. This TF variant was identified as TF DHR5, being identical with a variant TF DSaga, in a direct comparison with several known TF D variants by PAGIF. The isoelectric points (pIs) of holo-type (Fe(II) binding type) of TF DHR5 (= DSaga) and TF C were 5.6 and 5.4, respectively. After sialidase treatment, TF DHR5 (= DSaga) showed a single band migrating at a more basic position than TF C, thus suggesting that the pI difference between the two was due to amino acid substitution.
Ohshima
T
T
Department of Legal Medicine, Kanazawa University Faculty of Medicine, School of Medicine, Japan.
Takayasu
T
T
Umetsu
K
K
Fujita
M
M
Satoh
C
C
D000180:Acrylic Resins; D000328:Adult; D001788:Blood Grouping and Crossmatching; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006650:Histocompatibility Testing; D006801:Humans; D007525:Isoelectric Focusing; D008297:Male; D010334:Paternity; D014168:Transferrin / Q000235:genetics*
D000180:Acrylic Resins; D014168:Transferrin; C016680:polyacrylamide gels