Molecular cloning and characterization of rat trp homologues from brain.
Identification of trp (transient receptor potential) gene from Drosophila photoreceptor and subsequent molecular cloning of the human cDNA homologues suggest its participation in capacitative calcium entry (CCE) or so called store-operated Ca2+ channel (SOC). We identified five different trp-related amplifications of reverse-transcription-polymerase chain reaction (RT-PCR) from rat brain; these corresponded to mouse trp homologues, mtrp1,3,4,5,6 and were distributed in various tissues with multiple expression levels. Two cDNAs, homologous to Drosophila trp from rat brain, designated rtrp3 and rtrp6, were isolated and characterized. By RT-PCR analysis, mRNAs of rtrp3 and rtrp6 were found to be expressed differently in brain and other tissues. In situ hybridization analysis revealed that rtrp6 mRNA was preferentially expressed in hippocampal dentate gyrus and cortical layers II and III. Expression of rat TRP3 and TRP6 in COS cells revealed an increase in CCE, as compared to that in the mock-transfected COS cells of the control. Isolation of cDNAs of rat trp gene family provides a useful model for studying mechanism of CCE.
Mizuno
N
N
Department of Pharmacology, Hiroshima University School of Dentistry, Kasumi 1-2-3, Minami-ku, Hiroshima, 734-8553, Japan.
Kitayama
S
S
Saishin
Y
Y
Shimada
S
S
Morita
K
K
Mitsuhata
C
C
Kurihara
H
H
Dohi
T
T
D000818:Animals; D000878:Anthranilate Synthase / Q000235:genetics; D001483:Base Sequence; D001923:Brain Chemistry / Q000502:physiology*; D001933:Brain Stem / Q000737:chemistry; D019556:COS Cells; D002118:Calcium / Q000493:pharmacokinetics; D015220:Calcium Channels / Q000235:genetics* / Q000378:metabolism; D027682:Cation Transport Proteins; D002531:Cerebellum / Q000737:chemistry; D002540:Cerebral Cortex / Q000737:chemistry; D003001:Cloning, Molecular; D004330:Drosophila; D004791:Enzyme Inhibitors / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects / Q000502:physiology; D006624:Hippocampus / Q000737:chemistry; D006801:Humans; D017403:In Situ Hybridization; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D008636:Mesencephalon / Q000737:chemistry; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics; D009830:Olfactory Bulb / Q000737:chemistry; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D050052:TRPC Cation Channels; D019284:Thapsigargin / Q000494:pharmacology
D015220:Calcium Channels; D027682:Cation Transport Proteins; D004791:Enzyme Inhibitors; D009097:Multienzyme Complexes; D012333:RNA, Messenger; D029701:Saccharomyces cerevisiae Proteins; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; C489571:TRPC4 ion channel; C096351:transient receptor potential cation channel, subfamily C, member 1; D019284:Thapsigargin; D007209:Indole-3-Glycerol-Phosphate Synthase; D000878:Anthranilate Synthase; C087439:TRP3 protein, S cerevisiae; D002118:Calcium