Monoclonal antibody-resistant mutants selected with a respiratory syncytial virus-neutralizing human antibody fab fragment (Fab 19) define a unique epitope on the fusion (F) glycoprotein.
A recombinant human antibody fragment, designated RSV Fab 19, efficiently neutralizes respiratory syncytial virus (RSV). Here we report the results of our sequence analysis of antibody escape mutants that identified F glycoprotein amino acids critical for binding of human or murine RSV F-neutralizing antibodies.
Crowe
J E
JE
Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20892-0720, USA. james.e.crowe@vanderbilt.edu
Firestone
C Y
CY
Crim
R
R
Beeler
J A
JA
Coelingh
K L
KL
Barbas
C F
CF
Burton
D R
DR
Chanock
R M
RM
Murphy
B R
BR
D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal; D002460:Cell Line; D000939:Epitopes / Q000032:analysis*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D009500:Neutralization Tests; D017354:Point Mutation; D011994:Recombinant Proteins; D012136:Respiratory Syncytial Viruses / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014760:Viral Fusion Proteins / Q000032:analysis / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins; D014760:Viral Fusion Proteins