title: Staurosporine enhanced benzamide riboside-induced apoptosis in human multidrug-resistant promyelocytic leukemia cells (HL-60/VCR) in vitro.
abstract: The inosine monophosphate (IMP) dehydrogenase inhibitor benzamide riboside (BR) induced apoptosis (detected with the aid of flow cytometric identification of cells with sub-G0 DNA content and increased side angle light scatter) equally or slightly more intensively in the multidrug-resistant human promyelocytic leukemia cell line (HL-60/VCR: MDR-1 gene, Pgp positive) in comparison with the parental drug sensitive HL-60 cells. Staurosporine alone induced relatively low level of apoptosis in parental HL-60 cells but higher level (approximately 35%) of apoptosis in multidrug-resistant HL-60/VCR cells after 24 hour induction. The combination of benzamide riboside and staurosporine induced in both drug-sensitive and drug-resistant HL-60 cells a marked proportion of apoptotic cells already after short (6 hour) induction (more than 30% of apoptotic cells).
authors.affiliation: Department of Molecular Immunology, Cancer Research Institute, Slovak Academy of Sciences, Bratislava.
authors.forename: L
authors.initials: L
authors.lastname: Hunáková
authors.forename: J
authors.initials: J
authors.lastname: Duraj
authors.forename: D
authors.initials: D
authors.lastname: Romanová
authors.forename: L
authors.initials: L
authors.lastname: Novotný
authors.forename: J
authors.initials: J
authors.lastname: Sedlák
authors.forename: M R
authors.initials: MR
authors.lastname: Kelley
authors.forename: T
authors.initials: T
authors.lastname: Szekeres
authors.forename: H N
authors.initials: HN
authors.lastname: Jayaram
authors.forename: B
authors.initials: B
authors.lastname: Chorváth
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D053938:DNA Fragmentation; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000187:drug effects*; D006801:Humans; D007168:IMP Dehydrogenase / Q000037:antagonists & inhibitors; D015473:Leukemia, Promyelocytic, Acute / Q000473:pathology*; D009363:Neoplasm Proteins / Q000378:metabolism; D009705:Nucleosides / Q000494:pharmacology*; D019311:Staurosporine / Q000494:pharmacology*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D004791:Enzyme Inhibitors; D009363:Neoplasm Proteins; D009705:Nucleosides; C076145:3-(1-deoxyribofuranosyl)benzamide; D007168:IMP Dehydrogenase; D019311:Staurosporine