Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA.
The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization-tof mass spectrometry. Hybridomas secreting monoclonal antibody against glycyrrhizin were produced by fusing splenocytes immunized with a glycyrrhizin-bovine serum albumin conjugate with the HAT-sensitive mouse myeloma cell line P3-X63-Ag8-653. A very small cross-reaction appeared with glycyrrhetic acid monoglucuronide and glycyrrhetic acid. The full measuring range of the assay extends from 20 ng ml(-1) to 200 ng ml(-1) of glycyrrhizin.
Tanaka
H
H
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Shoyama
Y
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D019695:Glycyrrhizic Acid / Q000276:immunology* / Q000302:isolation & purification; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; D019695:Glycyrrhizic Acid