title: Prediction of radiosensitivity of oral cancers by serial cytological assay of nuclear changes.
abstract: OBJECTIVE
  To identify the relationship between the radiosensitivity of oral cancers and the induction of micronucleation, nuclear budding and multinucleation (polynucleation) evaluated by serial cytology during fractionated radiotherapy.
  
  
  METHODS
  Forty-four patients with epidermoid cancer of the oral cavity receiving radiotherapy (60 Gy in 25 fractions over 5 weeks) were studied. Serial scrape smears were taken from the tumour before and during radiotherapy and stained by Giemsa and the frequency of micronucleated cells (MNC), nuclear budded cells (NBC) and multinucleated cells (PNC) was evaluated by light microscopy. After a minimum follow-up period of 30 months the patients were classified as having resistant or sensitive tumours, depending on whether the primary tumour had recurred or not within that time. Within-group and between-group analysis on the induction of the above individual parameters and two combined parameters, the micro- or multinucleated cell (MPC) count and the abnormally nucleated cell (ANC) count, was done. The counts were expressed per 1000 uni-nucleated cells.
  
  
  RESULTS
  In both groups each parameter showed a statistically significant increase with dose, the increase being higher in the sensitive group. The ANC count showed the greatest increase, the mean counts before treatment and after 28.8 Gy being 24.3 and 157.8 (P < 0.0005), respectively, in the sensitive group and 21.0 and 65.2 (P < 0.0005), respectively, in the resistant group. After 28.8 Gy the sensitive tumours had significantly higher ANC (P = 0.01), MPC (P < 0.05) and PNC (P < 0.05) counts.
  
  
  CONCLUSIONS
  The study shows that serial cytological assay of nuclear changes (SCANCing) during radiotherapy is a potentially useful test to predict radiosensitivity. The fact that multinucleation showed the greatest relation with radiosensitivity suggests that injury to the cytokinetic apparatus is important in determining tumour radiosensitivity.
authors.affiliation: Department of Radiotherapy, Regional Cancer Centre, Trivandrum, India.
authors.forename: N V
authors.initials: NV
authors.lastname: Bhattathiri
authors.forename: C
authors.initials: C
authors.lastname: Bharathykkutty
authors.forename: R
authors.initials: R
authors.lastname: Prathapan
authors.forename: D A
authors.initials: DA
authors.lastname: Chirayathmanjiyil
authors.forename: K M
authors.initials: KM
authors.lastname: Nair
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000532:radiotherapy* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000528:radiation effects*; D003584:Cytological Techniques; D019583:Dose Fractionation, Radiation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000532:radiotherapy* / Q000648:ultrastructure; D011836:Radiation Tolerance; D011879:Radiotherapy Dosage