A helper-independent adenovirus vector with E1, E3, and fiber deleted: structure and infectivity of fiberless particles.
The adenovirus (Ad) fiber protein largely determines viral tropism through interaction with specific cell surface receptors. This molecule may also be involved in virion assembly or maturation, as some previously characterized fiber mutants were defective for processing of viral structural proteins. We previously described packaging cell lines that express Ad type 5 (Ad5) fiber and can complement the temperature-sensitive Ad fiber mutant H5ts142. We have now used these packaging cells to construct a new adenoviral vector (Ad5.betagal.DeltaF) with E1, E3, and L5 (fiber) deleted and analyzed the fiber null phenotype. Ad5.betagal.DeltaF growth was completely helper independent, and fiberless particles were produced by a single final round of growth in 293 cells. Cryoelectron microscopic studies and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis showed that the structure and composition of these particles was nearly identical to those of first-generation Ad vectors. As expected, fiberless particles had reduced infectivity on epithelial cells, but they retained the ability to infect monocytic cells via an integrin-dependent pathway. These studies provide a novel approach to developing retargeted Ad gene therapy vectors.
Von Seggern
D J
DJ
Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
Chiu
C Y
CY
Fleck
S K
SK
Stewart
P L
PL
Nemerow
G R
GR
D017498:Adenovirus E1 Proteins / Q000235:genetics; D017503:Adenovirus E3 Proteins / Q000235:genetics; D000260:Adenoviruses, Human / Q000254:growth & development / Q000502:physiology*; D002213:Capsid / Q000235:genetics; D036022:Capsid Proteins; D002460:Cell Line; D002461:Cell Line, Transformed; D017353:Gene Deletion; D005822:Genetic Vectors / Q000502:physiology*; D006378:Helper Viruses; D006801:Humans; D016296:Mutagenesis; D014771:Virion / Q000648:ultrastructure; D001616:beta-Galactosidase / Q000235:genetics
D017498:Adenovirus E1 Proteins; D017503:Adenovirus E3 Proteins; D036022:Capsid Proteins; C018315:hexon capsid protein, Adenovirus; D001616:beta-Galactosidase