title: Anti-viral effect of interferon-alpha on bovine viral diarrhea virus.
abstract: To get basic information to control persistent virus infection among domestic animals by cytokines, the antiviral activity of four natural human cytokines against bovine viral diarrhea virus (BVDV) was evaluated. Normal bovine peripheral blood mononuclear leukocytes (PBML) and fetal bovine muscular cells (FBMC) were treated with varying doses of human interferon (IFN)-alpha, IFN-gamma, tumor necrosis factor (TNF)-alpha and TNF-beta. The antiviral activity in treated cells was measured by the titration of virus infectivity in comparison with non-treated controls. IFN-alpha significantly suppressed virus growth in both PBML and FBMC. The growth of two cytopathogenic and two noncytopathogenic strains was suppressed in the presence of more than 10(3) u/ml of IFN-alpha. Addition of either TNF-alpha or TNF-beta to IFN-alpha did not potentiate the suppressive effect. IFN-alpha also suppressed the replication of BVDV in PBML from cattle persistently infected with BVDV.
authors.affiliation: National Institute of Animal Health, Ibaraki, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sentsui
authors.forename: R
authors.initials: R
authors.lastname: Takami
authors.forename: T
authors.initials: T
authors.lastname: Nishimori
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Y
authors.initials: Y
authors.lastname: Yokomizo
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000627:therapeutic use*; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000628:therapy*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000187:drug effects / Q000302:isolation & purification; D006801:Humans; D066298:In Vitro Techniques; D016898:Interferon-alpha / Q000627:therapeutic use*; D007371:Interferon-gamma / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000821:virology; D008233:Lymphotoxin-alpha / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D016898:Interferon-alpha; D008233:Lymphotoxin-alpha; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma