Comparative experimental study of Mg lactate, vitamin B6 and their association on the permeability of a human membrane. 2. Effects on cellular and paracellular ionic transfer through isolated amniotic membrane.
The comparative effects of Mg lactate, pyridoxine chlorhydrate and their association were observed on the various components of the human transamniotic conductance Gt. The use of both microelectrodes and metabolic inhibitors indicates 12 components of Gt: 8 cellular components Gc (Na-K, ATPase, Na-H and Cl-HCO3 antiports, Na-K-2Cl cotransport, Na-Mg exchanger and Na, K, Cl channels), one coupling component and three paracellular components Gp (Na, K, Cl). Mg lactate had a monophasic action on some components (decrease or increase conductance), according to its concentration, on maternal side (MS): GpNa, GpK, GpCl, K channels, conductance due to Na-K, ATPase. On fetal side (FS), there is a monophasic action on GpK, Na channels and a biphasic action (decrease then increase conductance) on GpNa and Na/Mg (as on MS). Pyridoxine chlorhydrate had a monophasic action on GpNa (MS, FS), GpK (MS), Na/Mg (MS, FS) and a biphasic action on GpK (FS), Na channels (MS, FS). The association of both Mg lactate and pyridoxine chlorhydrate implicated a biphasic action on all cationic components on both sides (GpNa, GpK, Na and K channels, Na/H, Na-K-2Cl, Na/Mg. The anionic components were not modified with regard to agents. The Mg lactate + vitamin B6 association interferes specifically with the cationic conductance components with regard to individual components.
Bara
M
M
Laboratory of Physiopathologie de l'Implantation et du Développement, University P. M. Curie, Paris, France. Michel.Bara@snv.jussieu.fr
Guiet-Bara
A
A
Durlach
J
J
D000650:Amnion / Q000187:drug effects* / Q000378:metabolism*; D017920:Antiporters / Q000187:drug effects / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D027963:Chloride-Bicarbonate Antiporters; D004594:Electrophysiology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007473:Ion Channels / Q000187:drug effects / Q000378:metabolism; D007477:Ions; D007773:Lactates / Q000494:pharmacology*; D017616:Magnesium Compounds / Q000378:metabolism / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects; D011247:Pregnancy; D011736:Pyridoxine / Q000378:metabolism / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000187:drug effects / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000187:drug effects / Q000378:metabolism
D017920:Antiporters; D027963:Chloride-Bicarbonate Antiporters; D007473:Ion Channels; D007477:Ions; D007773:Lactates; D017616:Magnesium Compounds; D017923:Sodium-Hydrogen Exchangers; C046250:sodium-magnesium antiporter; D000254:Sodium-Potassium-Exchanging ATPase; D011736:Pyridoxine