9-cis retinoic acid induces the expression of the uncoupling protein-2 gene in brown adipocytes.
The expression of uncoupling protein-2 (UCP2) mRNA is up-regulated during the differentiation of brown adipocytes in primary culture. When differentiation of brown adipocytes is impaired, UCP2 mRNA expression is down-regulated. 9-cis Retinoic acid causes a dose-dependent induction of UCP2 mRNA levels in brown adipocytes, whereas all-trans retinoic acid has no effect. Specific agonists of retinoid X receptors (RXR) induce UCP2 mRNA expression, whereas specific activators of retinoic acid receptors do not. 9-cis Retinoic acid, acting through RXR receptors, is identified as a major regulator of the expression of the UCP2 gene in the brown fat cell.
Carmona
M C
MC
Departament de Bioquimica i Biologia Molecular, Universitat de Barcelona, Spain.
Valmaseda
A
A
Iglesias
R
R
Mampel
T
T
Viñas
O
O
Giralt
M
M
Villarroya
F
F
D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000077556:Alitretinoin; D000818:Animals; D005786:Gene Expression Regulation / Q000187:drug effects*; D006728:Hormones / Q000819:agonists; D007473:Ion Channels; D026901:Membrane Transport Proteins; D051379:Mice; D024101:Mitochondrial Proteins; D011506:Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D014212:Tretinoin / Q000494:pharmacology*; D000071246:Uncoupling Protein 2
D006728:Hormones; D007473:Ion Channels; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; D012333:RNA, Messenger; C000604390:Ucp2 protein, mouse; D000071246:Uncoupling Protein 2; D000077556:Alitretinoin; D014212:Tretinoin