Genetic mapping of RAPD markers linked to the densonucleosis refractoriness gene, nsd-1, in the silkworm, Bombyx mori.
In the silkworm, Bombyx mori, nonsusceptibility to B. mori densonucleosis virus type-1 (BmDNV-1) is controlled by a recessive gene, nsd-1 (nonsusceptibility to DNV-1), located on the twenty-first chromosome. We investigated genetic linkage between five random amplified polymorphic DNA (RAPD) markers and the +nsd-1 gene. Initially, we constructed the CSD-1 strain (nsd-1/+) which is congenic to strain C137 (nsd-1/nsd-1) for the twenty-first chromosome, starting with a female of C137 and a male of strain J137 (+nsd-1/+nsd-1). For the crossing over experiment, a female of C137 was crossed with a male (nsd-1/+) of CSD-1. Segregation analysis showed that the most closely linked RAPD marker mapped 3.0 cM distant from +nsd-1. A more specific marker for +nsd-1 was made by converting this RAPD band into a sequence characterized amplified region (SCAR) using a series of newly designed primer pairs based on its DNA sequence.
Abe
H
H
Department of Biological Production, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Harada
T
T
Kanehara
M
M
Shimada
T
T
Ohbayashi
F
F
Oshiki
T
T
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000821:virology; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D004247:DNA; D005260:Female; D017344:Genes, Insect; D005808:Genes, Recessive; D005819:Genetic Markers; D007304:Insect Viruses / Q000502:physiology*; D008297:Male; D008969:Molecular Sequence Data; D017992:Parvovirus / Q000502:physiology*; D019105:Random Amplified Polymorphic DNA Technique
D005819:Genetic Markers; D004247:DNA