Anti-cytoplasmic autoantibodies reactive with epithelial cells of the salivary gland in sera from patients with Sjögren's syndrome: their disease- and organ-specificities.
To test whether the autoantibodies reactive with epithelial cells of the salivary gland in sera from Sjögren's syndrome (SS) patients are specific for the organ and the disease, tissue reactivities of serum IgG obtained from the patients with SS and oral lichen planus (OLP), another immune-mediated oral mucosal disease, were examined by immunohistochemistry and Western blotting. IgG purified from the sera of SS patients specifically localized not only on the nuclei but also on the cytoplasm of the salivary gland epithelial cells. On the other hand, no convincing staining of the epithelial cells was observed when IgG purified from the sera of OLP patients or those from healthy controls were used for immunohistochemistry. No cytoplasmic staining was observed when sections of kidneys and pancreas were stained with SS patients' IgG. In Western blotting performed by using lysates of a submandibular gland as antigens, all the IgG prepared from the SS patients reacted prominently with several protein bands, including those specific for the disease and the organ. These results suggest that production of autoantibodies reacting with the cytoplasm of salivary gland epithelial cells is a characteristic of SS, and may play a role in the pathogenesis of the sialadenitis.
Sakamoto
M
M
Department of Oral Diagnosis and Radiology, Tohoku University School of Dentistry, Sendai, Japan.
Miyazawa
M
M
Mori
S
S
Fujisawa
R
R
D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000096:biosynthesis / Q000097:blood / Q000276:immunology*; D015153:Blotting, Western; D002467:Cell Nucleus / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology*; D004847:Epithelial Cells / Q000276:immunology*; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007668:Kidney / Q000276:immunology; D017676:Lichen Planus, Oral / Q000097:blood / Q000276:immunology; D008739:Methyl Green; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology; D012394:Rosaniline Dyes; D012470:Salivary Glands, Minor / Q000276:immunology*; D012793:Sialadenitis / Q000276:immunology; D012859:Sjogren's Syndrome / Q000097:blood / Q000276:immunology*; D013045:Species Specificity
D001323:Autoantibodies; D007074:Immunoglobulin G; D012394:Rosaniline Dyes; D008739:Methyl Green