Induction of intercellular adhesion molecule 1 gene expression by measles virus in human umbilical vein endothelial cells.
The expression of intercellular adhesion molecule 1 (ICAM-1) by endothelial cells is important for the regulation of adhesion and transendothelial migration of a variety of leukocytes that express the integrins lymphocyte function-associated antigen 1 (LFA-1) and/or Mac-1. Here, we demonstrate strain-specific differences in the ability of measles virus (MV) to induce ICAM-1 expression. The vaccine strain Moraten (Mor) rapidly induced high levels of ICAM-1 mRNA and protein expression, whereas the vaccine strain CAM-70 and the Edmonston wild type (Ed-wt) strain were far less effective, even when they were used at very high multiplicities of infection (MOIs). Strain-specific differences in the induction were not a consequence of differences in the ability to infect ECs. Furthermore, induction of ICAM-1 by Mor was not dependent on de novo expression of MV or cellular proteins. Dual-immunofluorescence analysis indicated that there was no association between the expression of either MV nucleocapsid or hemagglutinin protein and the induction of ICAM-1 expression. Some human umbilical vein endothelial cells (HUVECs) that expressed high nucleocapsid protein in response to either Mor or CAM-70 failed to express elevated ICAM-1, whereas some HUVECs that were incubated with Mor expressed high ICAM-1 prior to expression of MV nucleocapsid protein. Strain-specific differences in the ability of Mor and CAM-70 to induce ICAM-1 correlated with their ability to activate the latent transcription factor NF-kappaB. These studies suggest a preexisting component of MV particles that leads to strain-specific differences in the activation of NF-kappaB and the induction of ICAM-1 gene expression.
Harcourt
B H
BH
Winship Cancer Center, Emory University, Atlanta, Georgia 30322, USA.
Rota
P A
PA
Hummel
K B
KB
Bellini
W J
WJ
Offermann
M K
MK
D000818:Animals; D015152:Blotting, Northern; D002522:Chlorocebus aethiops; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D004847:Epithelial Cells / Q000378:metabolism / Q000821:virology; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000378:metabolism; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008459:Measles virus / Q000254:growth & development / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000032:analysis / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D019590:Nucleocapsid Proteins / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism*; D014709:Vero Cells; D014764:Viral Proteins / Q000378:metabolism; D014765:Viral Vaccines / Q000494:pharmacology
D006389:Hemagglutinins, Viral; D008562:Membrane Glycoproteins; D016328:NF-kappa B; D019590:Nucleocapsid Proteins; D012333:RNA, Messenger; D014764:Viral Proteins; D014765:Viral Vaccines; D018799:Intercellular Adhesion Molecule-1