Targeting collecting tubules using the aquaporin-2 promoter.
The aquaporin-2 promoter has been used to drive Cre recombinase expression in order to achieve renal collecting duct principal cell specific gene deletion. This technique requires two lines of mice: one transgenic mouse line containing a cell-specific promoter driving Cre recombinase expression and the other line, engineered using gene targeting strategies, that contains a lox-flanked target gene of interest. Mating of these two mouse lines permits cell-specific deletion of the target gene. This method could ultimately be used to obtain targeted deletion of any gene in any cell type in the kidney for which a specific promoter has been identified. The applications of this technology, as well as its strengths and weaknesses, are discussed with particular reference to the kidney.
Stricklett
P K
PK
Salt Lake Veterans Affairs Medical Center and University of Utah, Salt Lake City, Utah 84148, USA.
Nelson
R D
RD
Kohan
D E
DE
D000818:Animals; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D019332:Endothelin-1 / Q000235:genetics; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D019426:Integrases / Q000235:genetics; D007685:Kidney Tubules, Collecting / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011401:Promoter Regions, Genetic; D014764:Viral Proteins
C496024:Aqp2 protein, mouse; D051399:Aquaporin 2; D051403:Aquaporin 6; D020346:Aquaporins; D017931:DNA Primers; D019332:Endothelin-1; D014764:Viral Proteins; C045073:Cre recombinase; D019426:Integrases