Molecular discrimination of enterotoxin C subtype in clinical isolates of Staphylococcus aureus.
The subtype of staphylococcal enterotoxin C (SEC) of 33 S. aureus clinical isolates was determined by polymerase chain reaction and direct DNA sequencing of a portion of the SEC gene encoding the SEC subtype-specific region. With the exception of a single strain with the SEC2 gene, all other strains showing different biologic and genetic properties were proved to possess the SEC3 gene.
Kobayashi
N
N
Dept. of Hygiene, Sapporo Medical University School of Medicine, Japan.
Okada
J
J
Kojima
K
K
Urasawa
S
S
Uehara
N
N
Watanabe
N
N
D017931:DNA Primers / Q000737:chemistry; D004587:Electrophoresis, Agar Gel; D004768:Enterotoxins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D007841:Latex Fixation Tests; D009500:Neutralization Tests; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013211:Staphylococcus aureus / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D018089:Superantigens / Q000737:chemistry / Q000235:genetics*
D017931:DNA Primers; D004768:Enterotoxins; D018089:Superantigens; C034959:enterotoxin C, staphylococcal