Regional variation in the lamina propria T cell receptor V beta repertoire in normal human colon.
Lamina propria (LP) T cell populations in the normal human colon contain oligoclonal expansions, but their distribution has not been well studied. We analyzed T cell receptor (TCR) beta-chain (V beta) variable region expression in CD4(+) and CD8(+) peripheral blood T cells and LP T cells from separated colonic segments in 13 subjects. CD4(+) and CD8(+) V beta subset expansions were found in the LP of most individuals, and remarkable differences in CD4(+) and CD8(+) TCR repertoires were apparent between colon and blood as well as between colon segments within each individual. The presence of such T cell expansions in colon therefore cannot be used to infer immunopathology. In addition, CD8(+) V beta expansions seen in peripheral blood T cells, which have been previously shown to be clonal in origin, were also often expanded in LP T cells of the same subject. These results suggest that LP CD8(+) T cell stimulation may contribute to CD8(+) peripheral blood T cell expansions.
Bennet
J D
JD
Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA.
Brown
W R
WR
Kotzin
B L
BL
D000368:Aged; D000369:Aged, 80 and over; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D003106:Colon / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D015333:Gene Rearrangement, beta-Chain T-Cell Antigen Receptor; D006801:Humans; D007135:Immunoglobulin Variable Region / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology* / Q000648:ultrastructure; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*
D007135:Immunoglobulin Variable Region; D016693:Receptors, Antigen, T-Cell, alpha-beta