The transcription factor GATA6 is essential for early extraembryonic development.
The gene coding for the murine transcription factor GATA6 was inactivated by insertion of a beta-galactosidase marker gene. The analysis of heterozygote GATA6/lacZ mice shows two inductions of GATA6 expression early in development. It is first expressed at the blastocyst stage in part of the inner cell mass and in the trophectoderm. The second wave of expression is in parietal endoderm (Reichert's membrane) and the mesoderm and endoderm that form the heart and gut. Inactivation leads to a lethality shortly after implantation (5.5 days postcoitum). Chimeric experiments show this to be caused by an indirect effect on the epiblast due to a defect in an extraembryonic tissue.
Koutsourakis
M
M
Erasmus University, Medical Genetics Centre, Department of Cell Biology and Genetics, The Netherlands.
Langeveld
A
A
Patient
R
R
Beddington
R
R
Grosveld
F
F
D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002454:Cell Differentiation; D002678:Chimera / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D005314:Embryonic and Fetal Development; D050993:GATA6 Transcription Factor; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D018390:Gene Targeting; D005804:Genes, Lethal / Q000235:genetics; D005838:Genotype; D006651:Histocytochemistry; D017403:In Situ Hybridization; D017404:In Situ Hybridization, Fluorescence; D007763:Lac Operon / Q000235:genetics; D051379:Mice; D011995:Recombination, Genetic / Q000235:genetics; D013234:Stem Cells / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics*; D001616:beta-Galactosidase / Q000235:genetics
D004268:DNA-Binding Proteins; D050993:GATA6 Transcription Factor; C494760:Gata6 protein, mouse; D014157:Transcription Factors; D001616:beta-Galactosidase