title: Receptor and non-receptor mediated formation of superoxide anion and hydrogen peroxide in neutrophils of intensive care patients.
abstract: Generation of reactive oxygen intermediates (ROI) has been implicated in tissue damage in a variety of disease states including sepsis and trauma. On the other hand, generation of ROI in polymorphonuclear granulocytes (PMN) presents a crucial element in the defence of the host against invading microorganisms. In the present study we investigated the generation of superoxide anions (O2-) and hydrogen peroxide (H2O2) by neutrophils (PMN)5 of 17 critically ill patients treated at a intensive care unit (ICU) after polytrauma (n = 6), heart operation (n = 6) or during septic shock (n = 5) using flow cytometry. O2- production of PMN from ICU patients was significantly lower (p < 0.01) than that in healthy volunteers (HV) during non-receptor mediated stimulation with phorbol-myristate-acetate (PMA) but higher (p < 0.001) during receptor mediated stimulation with formylmethionine-leucine-phenylalanine (FMLP). H2O2 generation of PMN from ICU patients was increased after stimulation with FMLP (p < 0.01) and remained unchanged after stimulation with PMA. Patients in septic shock had lower O2(-)-generation of PMN than did injured patients and patients after heart operations. We conclude that receptor mediated formation of O2- and H2O2 is stimulated in ICU patients. However, in patients in septic shock O2(-)-generation decreases, which potentially might contribute to the immunoparalysis present in septic shock.
authors.affiliation: Department of Surgery, AKH University Hospital, Vienna, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Manhart
authors.forename: C
authors.initials: C
authors.lastname: Oismüller
authors.forename: A
authors.initials: A
authors.lastname: Lassnig
authors.forename: A
authors.initials: A
authors.lastname: Spittler
authors.forename: T
authors.initials: T
authors.lastname: Sautner
authors.forename: P
authors.initials: P
authors.lastname: Götzinger
authors.forename: R
authors.initials: R
authors.lastname: Függer
authors.forename: E
authors.initials: E
authors.lastname: Roth
mesh_terms: D000328:Adult; D000368:Aged; D001026:Coronary Artery Bypass; D003422:Critical Care; D005260:Female; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009104:Multiple Trauma / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D011183:Postoperative Complications / Q000276:immunology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic / Q000276:immunology*; D018000:Receptors, Peptide / Q000276:immunology*; D016897:Respiratory Burst / Q000276:immunology; D012772:Shock, Septic / Q000276:immunology; D013481:Superoxides / Q000378:metabolism*; D018746:Systemic Inflammatory Response Syndrome / Q000276:immunology*
chemical_list: D017382:Reactive Oxygen Species; D044042:Receptors, Formyl Peptide; D011971:Receptors, Immunologic; D018000:Receptors, Peptide; D013481:Superoxides; D006861:Hydrogen Peroxide