Laser welding of vas deferens in rodents: initial experience with fluid solders.
This study evaluates the use of sutureless laser welding for vasovasostomy. In 14 rodents, the left vas deferens underwent vasovasostomy using an albumin-based solder applied to the adventitia of the vas deferens. The solder contained the dye, indocyanine green, to allow selective absorption and denaturation by a fiber-coupled 800-nm diode laser. The right vas deferens served as a control, receiving conventional layered microsurgical repair. We used a removable 4/0 nylon stent and microclamps to appose the vas deferens during repair, with no need for stay sutures. The mean time to perform laser solder repair (23.5 min) and conventional repair (23.3 min) were not significantly different (P=0.91). However, examination after 8 weeks showed that granuloma formation (G) and patency (P) rates for the conventional suture technique (G, 14%; P, 93%) were significantly better than observed for the laser solder technique (G, 57%; P, 50%).
Trickett
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RI
Microsearch Foundation of Australia, Sydney, NSW. trickett@macadam.mpce.mq.edu.au
Wang
D
D
Maitz
P
P
Lanzetta
M
M
Owen
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ER
D000418:Albumins / Q000008:administration & dosage*; D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D002417:Cattle; D004396:Coloring Agents / Q000008:administration & dosage; D004195:Disease Models, Animal; D007208:Indocyanine Green / Q000008:administration & dosage; D017075:Laser Coagulation / Q000295:instrumentation / Q000379:methods*; D008297:Male; D008866:Microsurgery / Q000295:instrumentation / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D012680:Sensitivity and Specificity; D013536:Suture Techniques; D016896:Treatment Outcome; D014649:Vas Deferens / Q000601:surgery*; D014659:Vasectomy / Q000379:methods*
D000418:Albumins; D001672:Biocompatible Materials; D004396:Coloring Agents; D007208:Indocyanine Green