Characterization of gene expression, genomic structure, and chromosomal localization of Hells (Lsh).
Hells (Lsh) is a lymphoid-specific presumptive helicase with highest expression in lymphoid precursor cells. Other members of the helicase family participate in maintenance of genome stability, DNA repair, and transcriptional control. Here we report the structure and chromosomal location of the Hells gene. The open reading frame of the murine Hells gene spans at least 26.6 kb of chromosomal DNA and is composed of 18 exons. The genomic structure of the seven helicase domains closely resembles that of mammalian Rad54, a gene whose product appears to be involved in recombination and double-strand break repair. The human homologue, the HELLS gene, has a mRNA expression pattern that is similar to murine Hells expression. Low-stringency hybridization in a Southern analysis reveals homologous Hells genes in a variety of species including Saccharomyces cerevisiae. FISH analysis maps the murine Hells gene to region C3-D1 on chromosome 19. The human homologue maps to a region of synteny on chromosome 10q23-q24, a breakpoint region frequently involved in human leukemia.
Geiman
T M
TM
Intramural Research Support Program, SAIC Frederick, National Cancer Institute, Frederick, Maryland, 21702-1201, USA.
Durum
S K
SK
Muegge
K
K
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism*; D002874:Chromosome Mapping; D002879:Chromosomes, Human, Pair 10; D017124:Conserved Sequence; D004265:DNA Helicases; D045643:DNA Repair Enzymes; D005091:Exons; D005656:Fungal Proteins / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009928:Organ Specificity; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid
D002352:Carrier Proteins; D005656:Fungal Proteins; D008565:Membrane Proteins; D029701:Saccharomyces cerevisiae Proteins; C075468:RAD54 protein, S cerevisiae; D004265:DNA Helicases; D045643:DNA Repair Enzymes