Estrogen causes cell death of estrogen receptor stably transfected cells via apoptosis.
We examined the effect of estrogen on the growth of estrogen receptor (ER) stably transfected cells (Rat1 + ER). 17-Beta-estradiol (E2, 10 nM) inhibited approximately 35-50% of Rat1 + ER growth after 3 d of treatment. The half-maximal growth inhibition occurred at 0.5-0.75 nM of E2 concentration and was saturated above 10 nM. This E2-induced antiproliferative effect was mediated through the ER since E2 did not cause any change in ER-negative parental Rat1 cells. Cells started to detach from plates and the adherent cells exhibited nuclear condensation. Apoptotic cell populations showed a 25% increase at 2 d of E2 treatment over controls that were quantified by fluorescence-activated cell sorter analysis. This indicates that E2 induced apoptosis in Rat1 + ER cells.
Lee
Y
Y
Department of Biochemistry, University of Wisconsin-Madison, 53706-1569, USA.
Renaud
R A
RA
Friedrich
T C
TC
Gorski
J
J
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002453:Cell Cycle; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000031:analogs & derivatives / Q000494:pharmacology; D004965:Estrogen Antagonists / Q000494:pharmacology; D004967:Estrogens / Q000502:physiology*; D005434:Flow Cytometry; D000077267:Fulvestrant; D051381:Rats; D011960:Receptors, Estrogen / Q000235:genetics*; D014162:Transfection
D004965:Estrogen Antagonists; D004967:Estrogens; D011960:Receptors, Estrogen; D000077267:Fulvestrant; D004958:Estradiol