A region of the cellobiohydrolase I promoter from the filamentous fungus Trichoderma reesei mediates glucose repression in Saccharomyces cerevisiae, dependent on mitochondrial activity.
The upstream activating region that controls cellulose-induced expression of the glucose-repressible cellobiohydrolase I gene (UARcb1) of the filamentous fungus Trichoderma reesei is shown to mediate transcription and glucose repression of a reporter gene in Saccharomyces cerevisiae, a unicellular microorganism that lacks the genes required for the utilization of cellulose. Glucose-controlled transcription mediated by UARcb1 requires the products of the genes SNF1 and SSN6, a protein kinase and a repressor, respectively, that regulate glucose-repressible yeast genes. Previously, it has been shown that mitochondrial function is implicated in cellobiohydrolase I gene expression in T. reesei and this sensitivity to the metabolic state of the mitochondria was shown to be transcriptionally controlled by the 5'-flanking sequence of the cbh1 gene [Abrahão-Neto et al. (1995) Biochemistry 34, 10456-10462]. Remarkably, transcription of the reporter gene controlled by UARcb1 in S. cerevisiae also showed a requirement for active mitochondria, suggesting that a common mechanism involving mitochondrial activity controls glucose-repressible genes in both microorganisms.
Carraro
D M
DM
Department of Biochemistry, Institute of Chemistry, University of São Paulo, Brazil.
Ferreira Júnior
J R
JR
Schumacher
R
R
Pereira
G G
GG
Hollenberg
C P
CP
El-Dorry
H
H
D020121:5' Untranslated Regions; D002480:Cellulase / Q000096:biosynthesis / Q000235:genetics*; D043366:Cellulose 1,4-beta-Cellobiosidase; D004268:DNA-Binding Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000235:genetics; D004794:Enzyme Repression / Q000235:genetics; D005656:Fungal Proteins / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D005947:Glucose / Q000502:physiology*; D006418:Heme / Q000502:physiology; D007763:Lac Operon; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*; D009687:Nuclear Proteins; D010101:Oxygen Consumption; D011401:Promoter Regions, Genetic; D017346:Protein Serine-Threonine Kinases / Q000502:physiology; D012097:Repressor Proteins; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics*
D020121:5' Untranslated Regions; C065455:CYC8 protein, S cerevisiae; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D006418:Heme; C071570:SNF1-related protein kinases; D017346:Protein Serine-Threonine Kinases; D002480:Cellulase; D043366:Cellulose 1,4-beta-Cellobiosidase; D005947:Glucose