Protective effect of histidine on MPP+-induced hydroxyl radical generation in rat striatum.
We investigated the efficacy of histidine on MPP+-induced hydroxyl radical (.OH) formation in extracellular fluid of rat striatum. Rats were anesthetized and sodium salicylate in Ringer's solution (0.5 nmol microl-1 min-1) was infused through a microdialysis probe to detect the generation of.OH as reflected by the nonenzymatic formation of 2,3-dihydroxybenzoic acid (DHBA) in the striatum. MPP+ (5 mM) clearly produced an increase in.OH formation. However, histidine (25 mM) reduced the.OH formation by the action of MPP+. These results indicate that histidine protects MPP+-induced.OH formation in rat striatum.
Obata
T
T
Department of Pharmacology, Oita Medical University, 1-1, Hasama-machi, Oita 879-5593, Japan.tobata@oita-med.ac.jp
Inada
T
T
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D003342:Corpus Striatum / Q000187:drug effects*; D006207:Half-Life; D006639:Histidine / Q000494:pharmacology*; D017665:Hydroxyl Radical; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009498:Neurotoxins / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
D018696:Neuroprotective Agents; D009498:Neurotoxins; D017665:Hydroxyl Radical; D006639:Histidine; D015655:1-Methyl-4-phenylpyridinium