CD1-restricted microbial lipid antigen-specific recognition found in the CD8+ alpha beta T cell pool.
It is generally accepted that TCR alphabeta+ CD8+ T cells recognize immunogenic peptides bound to MHC-encoded class I molecules. This recognition is a major component of the cellular response mediating immune protection and recovery from viral infections and from certain intracellular bacterial infections. Here, we report two human CD8+ TCR alphabeta+ T cell lines specific for Mycobacterium tuberculosis Ags presented in the context of CD1a or CD1c Ag-presenting molecules. These T cells recognize lipid Ags and display cytotoxicity as well as strong Th cell type I cytokine responses. By extending presentation by the CD1 system to the major TCR alphabeta+ CD8+ T cell pool, this system gains wider applicability beyond the double negative subset of T cells previously shown to have this reactivity. This implies that previous assumptions about the role of CD8+ T cells in microbial immunity may require revision as the relative proportions of CD1-restricted and MHC class I-restricted CD8+ T cells are further defined.
Rosat
J P
JP
Department of Medicine, Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Grant
E P
EP
Beckman
E M
EM
Dascher
C C
CC
Sieling
P A
PA
Frederique
D
D
Modlin
R L
RL
Porcelli
S A
SA
Furlong
S T
ST
Brenner
M B
MB
D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000276:immunology*; D018949:Antigens, CD1 / Q000502:physiology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D002460:Cell Line; D002850:Chromatography, Gel; D016207:Cytokines / Q000096:biosynthesis; D003601:Cytotoxicity Tests, Immunologic; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006017:Glycolipids / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D008055:Lipids / Q000276:immunology* / Q000302:isolation & purification; D008213:Lymphocyte Activation; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010743:Phospholipids / Q000276:immunology / Q000302:isolation & purification; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism*
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D018949:Antigens, CD1; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D006017:Glycolipids; D008055:Lipids; D010743:Phospholipids; D016693:Receptors, Antigen, T-Cell, alpha-beta