Characterisation of low free-energy excited states of folded proteins.
It is demonstrated that the identity of residues accessing excited conformational states that are of low free energy relative to the ground state in proteins can be obtained from amide proton NMR chemical shift temperature dependences displaying significant curvature. For the N-terminal domain of phosphoglycerate kinase, hen egg-white lysozyme and BPTI, conformational heterogeneity arises from a number of independent sources, including: structural instability resulting from deletion of part of the protein; a minor conformer generated through disulphide bond isomerisation; an alternative hydrogen bond network associated with buried water molecules; alternative hydrogen bonds involving backbone amides and surface-exposed side-chain hydrogen bond acceptors; and the disruption of loops, ends of secondary structural elements and chain termini. In many of these cases, the conformational heterogeneity at these sites has previously been identified by X-ray and/or NMR studies, but conformational heterogeneity of buried water molecules has hitherto received little attention. These multiple independent low free-energy excited states each involve a small number of residues and are shown to be within 2.5 kcal mol-1 of the ground state. Their relationship with the partially unfolded forms previously characterised using amide proton exchange studies is discussed.
Baxter
N J
NJ
Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Sheffield, Western Bank, S10 2TN, UK.
Hosszu
L L
LL
Waltho
J P
JP
Williamson
M P
MP
D000577:Amides / Q000737:chemistry; D007611:Aprotinin / Q000737:chemistry*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D009113:Muramidase / Q000737:chemistry*; D010735:Phosphoglycerate Kinase / Q000737:chemistry*; D011487:Protein Conformation; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry; D013696:Temperature
D000577:Amides; D011506:Proteins; D007611:Aprotinin; D010735:Phosphoglycerate Kinase; D009113:Muramidase