Simultaneous determination of 5-fluorouracil and uracil by high-performance liquid chromatography using four serial columns.
A sensitive assay was developed for the quantitation of 5-fluorouracil (5-FU) and uracil using liquid-liquid extraction (LLE) and HPLC with UV detection. Analyses were performed with four microBondapak C18 columns connected in series using 20 mM acetic acid with 1% ACN as mobile phase. The calibration curves were linear across the range of 26-1000 ng ml(-1) (0.21-7.8 microM) for 5-FU and 1.0-14.0 microg ml(-1) (0.01-110 microM) for uracil. This assay has been implemented to determine the plasma concentrations for pharmacokinetic studies for 5-FU and uracil in conjunction with clinical trials.
House
L K
LK
Department of Medicine, The University of Chicago, IL 60637, USA.
Ramírez
J
J
Ratain
M J
MJ
D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005472:Fluorouracil / Q000097:blood*; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D014498:Uracil / Q000097:blood*
D014498:Uracil; D005472:Fluorouracil