title: Heterologous expression of a basic elicitin from Phytophthora cryptogea in Phytophthora infestans increases its ability to cause leaf necrosis in tobacco.
abstract: The cry-b sequence, encoding a basic elicitin (cryptogein B) from Phytophthora cryptogea, was co-transformed into Phytophthora infestans. The copy number of the cry-b sequence varied in co-transformants. Nevertheless, in all cases the alien elicitin gene was transcribed, translated and the protein secreted in vitro from such transformants. Moreover, the secreted cryptogein B from P. infestans co-transformants increased their ability to cause a hypersensitive-response-like necrosis of tobacco leaves. It was thus concluded that the transfer of a single gene encoding a basic elicitin from one Phytophthora species to another can dramatically alter the phenotypic interaction of the transformed species with tobacco.
authors.affiliation: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Laurence
authors.initials: L
authors.lastname: Tetley
authors.affiliation: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Samantha M A
authors.initials: SMA
authors.lastname: Brown
authors.affiliation: Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
authors.forename: Vincent
authors.initials: V
authors.lastname: McDonald
authors.affiliation: Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
authors.forename: Graham H
authors.initials: GH
authors.lastname: Coombs
mesh_terms: D020418:Algal Proteins; D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence; D002934:Cinnamates; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D009336:Necrosis; D010838:Phytophthora / Q000235:genetics* / Q000472:pathogenicity; D010947:Plants, Toxic; D010957:Plasmids / Q000235:genetics; D011506:Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014026:Nicotiana / Q000382:microbiology*; D014170:Transformation, Genetic
chemical_list: D020418:Algal Proteins; D001426:Bacterial Proteins; D002934:Cinnamates; D005656:Fungal Proteins; D011506:Proteins; C061032:cryptogein protein, Phytophthora cryptogea; C085101:elicitin, Phytophthora; D006921:Hygromycin B; C026273:hygromycin A