DNA copy number changes in gastrointestinal stromal tumors--a distinct genetic entity.
It is not universally agreed whether gastrointestinal stromal tumors (GISTs) are phenotypical variants of leiomyomas (LM) and leiomyosarcomas (LMS), or whether they belong to a separate phenotypic entity with a different genetic background. This is a summary of our published results from comparative genomic hybridization studies performed in order to solve this question. We studied DNA copy number changes in 32 immunohistochemically well-defined GISTs (13 malignant, 3 borderline, and 16 benign tumors), 14 LM, and 29 LMS. In GISTs, a consistent finding was the loss of DNA copy numbers in chromosome 14q. This loss was detected in 75% of the benign tumors, in 62% of the malignant tumors, and in two out of the three borderline tumors with the minimal overlapping region located to 14q22. The loss was not seen in LM and in LMS it was rare (4 cases, 13%). Only three LM cases showed DNA copy number changes: gains in chromosome 3, 4, 5, 8, and 17. A total of 137 losses and 204 gains were detected in LMS. The most frequent losses were detected in 10q (20 cases, 69%) and 13q (17 cases, 59%). The most frequent gains were detected in 17p (16 cases, 55%). The most frequent high-level amplifications were detected in 17p (7 cases, 24%) and 8q (6 cases, 21%). Our results indicate that DNA copy number losses in 14q are an early change during the oncogenesis of GISTs. Moreover, the pattern of the demonstrated DNA copy number changes suggests that GISTs are a phenotypic and genetic entity separate from leiomyomas and leiomyosarcomas.
el-Rifai
W
W
Department of Medical Genetics, Haartman Institute, University of Helsinki, Finland.
Sarlomo-Rikala
M
M
Andersson
L C
LC
Miettinen
M
M
Knuutila
S
S
D002879:Chromosomes, Human, Pair 10 / Q000235:genetics; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics; D002898:Chromosomes, Human, Pair 8 / Q000235:genetics; D004273:DNA, Neoplasm / Q000235:genetics*; D005770:Gastrointestinal Neoplasms / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007889:Leiomyoma / Q000235:genetics; D007890:Leiomyosarcoma / Q000235:genetics; D008637:Mesenchymoma / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D012509:Sarcoma / Q000235:genetics
D004273:DNA, Neoplasm