Cytokine production in primary histoculture by human normal kidney, renal cell carcinoma and benign renal angiomyolipoma tissues.
In order to understand the ability of normal human kidney, benign renal angiomyolipoma and malignant renal cell carcinoma (RCC) cells to produce cytokines, we determined the IL-1-beta, IL-6, IL-10, IL-11, TNF-alpha and TGF-beta-1 concentration in the supernatant of histoculture specimens according to thymidine labelling indices. From these studies, we conclude that normal, benign and malignant renal tissues are one of the main sources of IL-6 production and patterns of IL-6 production are inversely proportional to thymidine labelling index in normal kidney(r = -0.9). However, IL-6 production is increased in proportion to the increasing thymidine labelling index in benign or malignant tissues (r = 0.94, r = 0.76). There is no production of IL-1-beta, IL-10, IL-11, TNF-alpha or TGF-beta-1. These findings allows many important studies of cytokines in normal, benign and malignant renal disease. Patterns of IL-6 production are different in normal and benign or malignant changed renal disease. There is no overlapping biologic effects among IL-6, IL-1-beta and TNF-alpha. Histoculture supports future studies of these cytokines.
Chang
S G
SG
Department of Urology, School of Medicine, Kyung Hee University Seoul, Korea.
Shin
C
C
Rho
S K
SK
Kim
D K
DK
Kim
J H
JH
D018207:Angiomyolipoma / Q000276:immunology* / Q000473:pathology; D002292:Carcinoma, Renal Cell / Q000276:immunology* / Q000473:pathology; D002455:Cell Division; D016207:Cytokines / Q000096:biosynthesis*; D004261:DNA Replication; D006801:Humans; D007375:Interleukin-1 / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D017370:Interleukin-11 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007672:Kidney Cortex / Q000166:cytology / Q000276:immunology*; D007680:Kidney Neoplasms / Q000276:immunology* / Q000473:pathology; D008940:Mitotic Index; D009924:Organ Culture Techniques; D012016:Reference Values; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000096:biosynthesis; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D007375:Interleukin-1; D017370:Interleukin-11; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D013936:Thymidine