Frequent occurrence of transgene deletion in transgenic plants.
The status of the transgene in tobacco plants transformed by Agrobacterium was analyzed with PCR. Twelve percent of the transgenic plants with the nptII gene showed different levels of transgene deletion, which was also found in transgenic watermelon (10-30%) and carrot (40-60%). It appeared that the percentage of transgenic plants carrying deleted transgenes depended on both the transgene and the plant. It is suggested that the transgene should be inserted between a right border and a selection marker to reduce the number of transgenic plants containing deleted transgenes after selection.
Kim
Y S
YS
Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
Lee
M H
MH
Min
S R
SR
Yoo
O J
OJ
Liu
J R
JR
D016960:Agrobacterium tumefaciens / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D017353:Gene Deletion; D051599:Glucose-1-Phosphate Adenylyltransferase; D009713:Nucleotidyltransferases / Q000235:genetics; D030821:Plants, Genetically Modified / Q000235:genetics*; D010947:Plants, Toxic; D011198:Solanum tuberosum / Q000201:enzymology / Q000235:genetics; D014026:Nicotiana / Q000201:enzymology / Q000235:genetics
D004269:DNA, Bacterial; D018076:DNA, Complementary; D018744:DNA, Plant; C036483:T-DNA; D009713:Nucleotidyltransferases; D051599:Glucose-1-Phosphate Adenylyltransferase