A yeast artificial chromosome-based physical map of the juvenile amyotrophic lateral sclerosis (ALS2) critical region on human chromosome 2q33-q34.
The autosomal recessive form of juvenile amyotrophic lateral sclerosis (ALS2; RFALS Type 3) has previously been mapped to the 8-cM interval flanked by D2S115 and D2S155 on human chromosome 2q33-q34. We have established a yeast artificial chromosome (YAC) contig spanning an approximately 8-Mb region of the ALS2 candidate region and mapped 52 transcribed DNA sequences including 13 known genes and 39 expressed sequenced tags within this YAC contig. The establishment of a YAC contig and transcript map that spans the region containing the ALS2 mutation is an essential step in the identification of the ALS2 gene.
Hadano
S
S
Department of Medical Genetics and Centre for Molecular Medicine and Therapeutics, University of British Columbia, 3024-980 West 28th Avenue, Vancouver, British Columbia, V5Z 4H4, Canada.
Nichol
K
K
Brinkman
R R
RR
Nasir
J
J
Martindale
D
D
Koop
B F
BF
Nicholson
D W
DW
Scherer
S W
SW
Ikeda
J E
JE
Hayden
M R
MR
D000690:Amyotrophic Lateral Sclerosis / Q000235:genetics*; D018244:Chromosomes, Artificial, Yeast; D002889:Chromosomes, Human, Pair 2; D020451:Contig Mapping; D006801:Humans