RNA aptamers that specifically bind to the Ras-binding domain of Raf-1.
RNA aptamers that bind to the Ras-binding domain (RBD) of a proto-oncogene product, Raf-1, were isolated from a pool of random sequences using a glutathione S-transferase-fused RBD (GST-RBD). The RNA molecules bind to the GST-RBD, but not to GST, with dissociation constants of about 300 nM. In contrast, these RNA aptamers do not bind to the Ras-binding domain of the RGL protein, which is also known to be activated by Ras. The aptamers actually compete with Ras for binding to the Raf-1 RBD. The anti-Raf-1 aptamers may be used to specifically inhibit the Ras-Raf interaction in the complicated signaling network in mammalian cells.
Kimoto
M
M
Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Japan.
Sakamoto
K
K
Shirouzu
M
M
Hirao
I
I
Yokoyama
S
S
D001483:Base Sequence; D001704:Biopolymers / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D019908:Proto-Oncogene Proteins c-raf / Q000737:chemistry / Q000378:metabolism*; D012313:RNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D018631:ras Proteins / Q000378:metabolism*
D001704:Biopolymers; D012313:RNA; D019908:Proto-Oncogene Proteins c-raf; D018631:ras Proteins