title: Biotin-conjugated polychelating agent.
abstract: A procedure is described for preparing a polychelating agent-biotin conjugate through the interaction of biotin-maleimide with poly-L-lysine modified with multiple residues of diethylenetriaminepentaacetic acid and containing amino-terminal pyridylthio-propionate group. The conjugate can be easily loaded with multiple metal atoms, such as 111In, and preserves its ability to specifically interact with avidin (as avidin-agarose). Polychelate-biotin conjugate may be used for signal amplification in visualization and analytical protocols involving the application of the avidin-biotin system.
authors.affiliation: Center for Imaging and Pharmaceutical Research, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 149 13th Street, Charlestown, Massachusetts 02129, USA.
authors.forename: V P
authors.initials: VP
authors.lastname: Torchilin
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D002614:Chelating Agents / Q000138:chemical synthesis / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D007205:Indium Radioisotopes; D008301:Maleimides; D004369:Pentetic Acid / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D002614:Chelating Agents; D007205:Indium Radioisotopes; D008301:Maleimides; D011107:Polylysine; D001710:Biotin; D004369:Pentetic Acid