mRNA differential display analysis of nephrotic kidney glomeruli.
BACKGROUND
Differential display RT-PCR (DDRT-PCR) is a new powerful technique for identification and characterization of altered gene expression in eukaryotic cells and tissues. We studied here changes in kidney glomerular gene expression in patients with congenital nephrotic syndrome of the Finnish type (CNF), an inherited kidney disease with heavy proteinuria already in utero.


METHODS
Using the DDRT-PCR approach and isolated glomeruli from removed human kidneys, we compared the gene expression patterns of normal human and CNF glomeruli. Differential expression of candidate genes was verified by Northern blotting, and the corresponding PCR fragments were sequenced and compared to known sequences in databanks.


RESULTS
We found several genes and sequence tags with altered expression in nephrotic glomeruli including fragments with close homologies to cytochrome c oxidase subunit I, integrin-linked kinase, insulin-like growth factor II receptor and eotaxin, and also clones resembling anchyrin and cadherin-like consensus sequences.


CONCLUSIONS
All the sequences identified are of interest in respect to pathogenesis of proteinuria. Furthermore, this study reveals potentially new members to known gene families with tissue and cell type-specific expression.
Haltia
A
A
Haartman Institute, Division of Bacteriology and Immunology, University of Helsinki, Finland.
Solin
M
M
Luimula
P
P
Kretzler
M
M
Holthöfer
H
H
D000328:Adult; D001483:Base Sequence; D016022:Case-Control Studies; D054413:Chemokine CCL11; D019742:Chemokines, CC; D002648:Child; D016207:Cytokines / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression; D006801:Humans; D017403:In Situ Hybridization; D007223:Infant; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000151:congenital / Q000235:genetics*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics*; D017527:Receptor, IGF Type 2 / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D000099200:Scaffold Protein ILK
D054413:Chemokine CCL11; D019742:Chemokines, CC; D016207:Cytokines; D017931:DNA Primers; D003576:Electron Transport Complex IV; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D017527:Receptor, IGF Type 2; D000099200:Scaffold Protein ILK; C515456:CCL11 protein, human