title: Complementation of the growth defect of an rnpA49 mutant of Escherichia coli by overexpression of arginine tRNA(CCG).
abstract: We previously found that overexpression of arginine tRNA(CCG) from Brevibacterium albidum complements the rnpA49 mutation, which is responsible for the thermosensitivity of Escherichia coli RNase P function. In this present work, we show that the E. coli homologue tRNA also complements the same mutation, but other tRNAs do not. These results suggest that the rnpA49 mutation causes a major cellular defect in an RNase P reaction to generate the mature arginine tRNA(CCG).
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon, Korea.
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.forename: B H
authors.initials: BH
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lee
authors.forename: J H
authors.initials: JH
authors.lastname: Chung
authors.forename: Y
authors.initials: Y
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000737:chemistry / Q000235:genetics*; D012361:RNA, Transfer, Pro / Q000737:chemistry / Q000235:genetics; D043262:Ribonuclease P; D013816:Thermodynamics
chemical_list: D017931:DNA Primers; D029968:Escherichia coli Proteins; D016337:RNA, Catalytic; D012347:RNA, Transfer, Arg; D012361:RNA, Transfer, Pro; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli