The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells.
Testing of the effects of xenobiotics in cultured cells often requires the use of organic solvents to effect suspension of the test agents in cell culture media. However, the toxic effects of the solvents themselves may introduce artifacts, which obscure interpretation of the experimental results. In this article, the toxicity of different solvents commonly used for solvation of a variety of xenobiotic agents was studied. We show that ethanol, acetone, isooctane, methanol, and hexane were considerably less toxic than the more commonly used solvent, DMSO, when ATP content and growth rates of HeLa cells exposed to these solvents was measured.
Forman
S
S
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic.
Kás
J
J
Fini
F
F
Steinberg
M
M
Ruml
T
T
D000096:Acetone / Q000633:toxicity; D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000633:toxicity; D000431:Ethanol / Q000633:toxicity; D006367:HeLa Cells / Q000187:drug effects*; D006586:Hexanes / Q000633:toxicity; D006801:Humans; D000432:Methanol / Q000633:toxicity; D009795:Octanes / Q000633:toxicity; D012997:Solvents / Q000633:toxicity*; D015262:Xenobiotics / Q000633:toxicity*
D006586:Hexanes; D009795:Octanes; D012997:Solvents; D015262:Xenobiotics; D000096:Acetone; D000431:Ethanol; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; C045798:2,2,4-trimethylpentane; D000432:Methanol; D004121:Dimethyl Sulfoxide