title: Relatedness of Streptococcus suis serotype 2 isolates from different geographic origins as evaluated by molecular fingerprinting and phenotyping.
abstract: The genetic diversity of 88 Streptococcus suis serotype 2 isolates which were recovered from various countries was examined by randomly amplified polymorphic DNA (RAPD) analysis with three primers. This bacterial collection included 80 isolates of porcine origin and 8 of human origin. This investigation allowed the identification of 23 RAPD types containing 1 to 30 isolates originating from one to six countries. Common RAPD patterns were found between human and pig isolates. The isolates were also tested for the production of virulent factors such as hemolysin, muramidase-released protein (MRP), and extracellular factor (EF). All isolates exhibiting the virulent phenotype hemolysin+ MRP+ EF+ clearly clustered on the basis of fingerprinting by RAPD analysis. In a similar way, most of isolates with the hemolysin- MRP- EF- phenotype were assigned to one RAPD cluster. Therefore, RAPD clusters are more related to the phenotype defined with hemolysin, MRP, and EF than to the geographic origin of the isolates. These data indicate that RAPD analysis used in conjunction with phenotypic methods provides a reliable method for the assessment of the clonal relationship between S. suis isolates responsible for infections in pigs or humans, especially for those exhibiting the classic "virulent" phenotype hemolysin+ MRP+ EF+.
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, St-Hyacinthe, Québec, Canada J2S 7C6.
authors.forename: S
authors.initials: S
authors.lastname: Chatellier
authors.forename: M
authors.initials: M
authors.lastname: Gottschalk
authors.forename: R
authors.initials: R
authors.lastname: Higgins
authors.forename: R
authors.initials: R
authors.lastname: Brousseau
authors.forename: J
authors.initials: J
authors.lastname: Harel
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D002170:Canada; D016000:Cluster Analysis; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D005060:Europe; D014644:Genetic Variation; D006460:Hemolysin Proteins / Q000378:metabolism; D006801:Humans; D010641:Phenotype; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique; D012703:Serotyping; D013290:Streptococcal Infections / Q000382:microbiology* / Q000662:veterinary; D017011:Streptococcus suis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014481:United States; D014774:Virulence
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D006460:Hemolysin Proteins