Analytical method for the determination of sphinganine and sphingosine in serum as a potential biomarker for fumonisin exposure.
The toxins produced by Fusarium moniliforme, which include fumonisins, are possible human carcinogens. Fumonisins are inhibitors of de novo sphingolipid biosynthesis. Alterations of the ratio of sphinganine (Sa) to sphingosine (So) in urine and serum has been proposed as a possible biomarker of exposure to this toxin. A new method was developed for their analysis in tissues and urine. This work describes the further adaptation of the method to the analysis of Sa and So in serum and its validation in sera of untreated and fumonisin B1 (FB1 ) treated rats and mice. No significant differences in the Sa/So ratios were observed in the FB1 treated rats. In mice, the increase was only of marginal statistical significance. Determination of Sa/So ratios in human sera could readily be made in small volumes (from 0.3 to 0.5 ml) of serum.
Castegnaro
M
M
Unit of Gene-Environment Interactions, International Agency for Research on Cancer, Lyon, France.
Garren
L
L
Galendo
D
D
Gelderblom
W C
WC
Chelule
P
P
Dutton
M F
MF
Wild
C P
CP
D000818:Animals; D015415:Biomarkers / Q000097:blood*; D002264:Carboxylic Acids / Q000633:toxicity*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D037341:Fumonisins; D006801:Humans; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D051381:Rats; D013050:Spectrometry, Fluorescence; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000097:blood*
D015415:Biomarkers; D002264:Carboxylic Acids; D037341:Fumonisins; C056933:fumonisin B1; D013110:Sphingosine; C005682:safingol