Elevation of alanine amino transferase and aspartate amino transferase produced by pyoverdin, a photolabile pigment of Pseudomonas fluorescens.
The effect of three forms pyoverdin on mouse liver was studied. Significant increases of alanine amino transferase (ALT) and aspartate amino transferase (AST) were obtained in mice after ingestion of water with forms A and C. The effect on liver was more evident with A than with C. Pyoverdin was purified by means of salt saturation, solvent extractions and ion-exchange chromatography. Fluorescent peaks obtained in the presence of light were different from those eluted under dark conditions. The relative amounts of pyoverdin A, B and C varied when dark purification procedure was employed. Form A decreased while C increased in the absence of light. Optimum conditions for C were in the dark without iron. When C was exposed to light, it changed to form A. Fast Atom Bombardment (FAB) mass spectrometry of pyoverdin form C gave a form at M+ = 1324 m.u., which is 9 m.u. less than pyoverdin purified in the presence of light. The results suggest that light can influence pyoverdin stability and toxicity.
Eraso
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AJ
Departmento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico Químicas y Naturales, Universidad Nacional de Río Cuarto, Argentina.
Albesa
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I
D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000528:radiation effects / Q000633:toxicity*; D002852:Chromatography, Ion Exchange; D004355:Drug Stability; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides; D010782:Photolysis; D010860:Pigments, Biological / Q000302:isolation & purification / Q000528:radiation effects / Q000633:toxicity*; D011551:Pseudomonas fluorescens / Q000737:chemistry*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D001426:Bacterial Proteins; D009842:Oligopeptides; D010860:Pigments, Biological; C042453:pyoverdin; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase