Differences in expression of oncogenes and tumor suppressor genes in different sites of head and neck squamous cell.
BACKGROUND
In most studies concerning chromosomal changes or protein expression in head and neck squamous cell carcinomas (HNSCC) no distinction is made between the sites within this area. The behaviour of tumors arising in one site or the other, however, differs significantly, suggesting different intrinsic tumor properties. In this study we compared the expression of several proteins (p53, Rb, cyclin D1 myc, bcl-2, EGFR, neu, E-Cadherin, Ep-CAM, Desmoplakin1 and nm23) in the three major sites of HNSCC (larynx, pharynx, and oral cavity).


METHODS
Expression of the proteins was studied by immunohistochemistry in 33 laryngeal, 31 pharyngeal and 36 oral carcinomas.


RESULTS
Cyclin D1 had a very high level of expression in the pharynx (p = 0.0004) and EGFR very low in the larynx (p < 0.0001) compared to the other sites. To a lesser degree p53 (p = 0.05), Desmoplakin1 (p = 0.04) and Ep-CAM (p = 0.02) showed statistically nearly significant differences. For the other proteins no statistically significant difference in expression was found.


CONCLUSIONS
These results show that the expression of a number of proteins is not identical in the three major localizations of HNSCC and indicate the different tumor biology of cancers originating from different sites in the Head and Neck. It is therefore not justified to consider the different localizations as one entity. Moreover, these differences may have clinical and prognostic relevance.
Takes
R P
RP
Department of Otolaryngology, Leiden University Medical Center, The Netherlands.
Baatenburg de Jong
R J
RJ
Schuuring
E
E
Litvinov
S V
SV
Hermans
J
J
Van Krieken
J H
JH
D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006258:Head and Neck Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000473:pathology; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology; D009363:Neoplasm Proteins / Q000032:analysis; D009367:Neoplasm Staging; D009857:Oncogenes; D010610:Pharyngeal Neoplasms / Q000235:genetics / Q000473:pathology; D012189:Retrospective Studies
D009363:Neoplasm Proteins