title: Regulation of mu binding sites after chronic administration of antibodies directed against specific anti-opiate peptides.
abstract: There is some indication that anti-opiate peptides (AOP) modulate opioid receptor systems by altering mu-receptor density. To further characterize this phenomenon, we investigated the effects of continuous infusion of anti-AOP IgG on mu binding sites in the brains of rats. Specifically, male Sprague-Dawley rats received intracerebroventricular (i.c.v.) infusions for 13 days of either control (rabbit) IgG or test IgGs: anti-dynorphin A IgG, anti-dynorphin A1-8 IgG, anti-alpha-MSH IgG, or the monoclonal anti-NPFF IgG. Administration of anti-NPFF IgG or the anti-dynorphin1-8 IgG significantly increased mu labeling by 40-70% in several brain regions at the caudate level. Contrary to these findings, anti-alpha-MSH IgG decreased (19-32%) [125I]-DAMGO labeling in several thalamic nuclei. The results suggest that the density of mu-opioid receptors is regulated in part by anti-opiate peptides in the extracellular fluid of the brain.
authors.affiliation: College of Pharmacy and Pharmaceutical Sciences, Florida A&M University, Tallahassee, USA. cgoodman@famu.edu
authors.forename: C B
authors.initials: CB
authors.lastname: Goodman
authors.forename: S
authors.initials: S
authors.lastname: Heyliger
authors.forename: B
authors.initials: B
authors.lastname: Emilien
authors.forename: J S
authors.initials: JS
authors.lastname: Partilla
authors.forename: H Y
authors.initials: HY
authors.lastname: Yang
authors.forename: C H
authors.initials: CH
authors.lastname: Lee
authors.forename: J L
authors.initials: JL
authors.lastname: Cadet
authors.forename: R B
authors.initials: RB
authors.lastname: Rothman
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology*; D001665:Binding Sites; D001923:Brain Chemistry; D004399:Dynorphins / Q000276:immunology; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D004745:Enkephalins; D000939:Epitopes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000276:immunology / Q000494:pharmacology; D007276:Injections, Intraventricular; D007457:Iodine Radioisotopes; D008297:Male; D018847:Opioid Peptides / Q000276:immunology* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017450:Receptors, Opioid, mu / Q000819:agonists / Q000378:metabolism* / Q000502:physiology; D000521:alpha-MSH / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D004745:Enkephalins; D000939:Epitopes; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D018847:Opioid Peptides; D017450:Receptors, Opioid, mu; D020875:Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; D000521:alpha-MSH; D004399:Dynorphins