A dilatancy assay for nucleoid denaturation: the centrifugation-dependent clumping of denatured spermidine nucleoids.
The ability of low centrifugal forces to convert denatured spermidine nucleoids from Escherichia coli into nonsedimentable macroscopic clumps is the basis of a rapid and simple, nonisotopic assay for nucleoid denaturation.
Murphy
L D
LD
Laboratory of Molecular Biology, National Institutes of Health, Bethesda, Maryland, 20892, USA.
Zimmerman
S B
SB
D001671:Biochemistry / Q000379:methods*; D002498:Centrifugation / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry* / Q000648:ultrastructure*; D009691:Nucleic Acid Denaturation; D015388:Organelles / Q000235:genetics / Q000648:ultrastructure; D013095:Spermidine / Q000737:chemistry; D014508:Urea / Q000737:chemistry
D004269:DNA, Bacterial; D014508:Urea; D013095:Spermidine