[Study of the interaction of MTPN11 mycobacteriophage with host cells based on electron microscopy, fluorimetry, and electro-oriented spectroscopy].
According to electron-microscopic data, various cells in the M. smegmatis ATCC607 population interact differently with phage MTPH11. Fluorometric studies of phage-host interactions were performed using a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfonate (ANS). Changes in the electric characteristics of mycobacterial cells infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spectroscopy for rapid phage typing of mycobacteria is discussed.
Zhilenkov
E L
EL
State Research Center for Applied Microbiology, Obolensk, Russia.
Shemiakin
I G
IG
Fomchenkov
V M
VM
Ivanov
A Iu
AIu
Gavriushkin
A V
AV
Oborotov
M V
MV
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; D005470:Fluorometry; D008854:Microscopy, Electron; D009160:Mycobacteriophages / Q000502:physiology* / Q000648:ultrastructure; D019907:Mycobacterium fortuitum / Q000821:virology; D020102:Mycobacterium smegmatis / Q000821:virology; D013057:Spectrum Analysis / Q000379:methods
D000817:Anilino Naphthalenesulfonates; D005456:Fluorescent Dyes; C027132:1-anilino-8-naphthalenesulfonate