New cloning vectors with temperature-sensitive replication.
A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by alpha complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired.
Phillips
G J
GJ
Department of Microbiology, Iowa State University, Ames, Iowa, 50011, USA.gregory@iastate.edu
D003001:Cloning, Molecular / Q000379:methods*; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics* / Q000378:metabolism; D015183:Restriction Mapping; D013696:Temperature