title: Pulsed-field gel electrophoresis is more efficient than ribotyping and random amplified polymorphic DNA analysis in discrimination of Pasteurella haemolytica strains.
abstract: One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA' to HT', that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1' to Rp 15') unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.
authors.affiliation: Ecole Nationale Vétérinaire de Lyon, F-69280 Marcy l'Etoile, France. a.kodjo@vet-lyon.fr
authors.forename: A
authors.initials: A
authors.lastname: Kodjo
authors.forename: L
authors.initials: L
authors.lastname: Villard
authors.forename: C
authors.initials: C
authors.lastname: Bizet
authors.forename: J L
authors.initials: JL
authors.lastname: Martel
authors.forename: R
authors.initials: R
authors.lastname: Sanchis
authors.forename: E
authors.initials: E
authors.lastname: Borges
authors.forename: D
authors.initials: D
authors.lastname: Gauthier
authors.forename: F
authors.initials: F
authors.lastname: Maurin
authors.forename: Y
authors.initials: Y
authors.lastname: Richard
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D020459:Genes, rRNA; D016978:Mannheimia haemolytica / Q000145:classification* / Q000235:genetics*; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012335:RNA, Ribosomal / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D015183:Restriction Mapping
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal