title: Promoter analysis of the Drosophila genes encoding TFIIB and TATA box-binding protein.
abstract: We have analyzed the 5'-flanking regions of the Drosophila genes encoding the TFIIB and TATA box-binding proteins (TBP) and mapped the regulatory regions required for their efficient expression. We found that the 500 bp long region (-439 to +60) and the 401 bp long region (-261 to +138) are required for the efficient expression of TFIIB and TBP genes, respectively. In the TFIIB promoter region, the upstream DNA between -439 and -280 and the downstream DNA between +8 and +60 are necessary for the stimulation of promoter activity. The upstream DNA between -439 and -280 stimulates transcription in an orientation dependent manner. In the TBP promoter region, the upstream DNA between -261 and -207, and the downstream DNA between +15 and +138 are necessary for the stimulation of promoter activity. The upstream DNA (-261 to -207) required for TBP promoter activity contains a 11 bp long palindromic sequence and a DNA replication-related element sequence. Particularly, we could find that the downstream promoter regions of TFIIB and TBP genes contain the conserved nucleotide sequences, suggesting the presence of a common regulatory mechanism for the expression of these two genes.
authors.affiliation: Department of Microbiology, Taejon University, Korea.
authors.forename: N
authors.initials: N
authors.lastname: Cho
authors.forename: Y
authors.initials: Y
authors.lastname: Oh
authors.forename: S Y
authors.initials: SY
authors.lastname: Hwang
authors.forename: D
authors.initials: D
authors.lastname: Han
authors.forename: S P
authors.initials: SP
authors.lastname: Park
authors.forename: J
authors.initials: J
authors.lastname: Yoon
authors.forename: K
authors.initials: K
authors.lastname: Han
authors.forename: K
authors.initials: K
authors.lastname: Baek
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics*; D004330:Drosophila / Q000166:cytology / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D017384:Sequence Deletion; D012689:Sequence Homology, Nucleic Acid; D016385:TATA Box; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; D011993:Recombinant Fusion Proteins; D035181:TATA-Box Binding Protein; D035581:Transcription Factor TFIIB; D014157:Transcription Factors; D004247:DNA; D008156:Luciferases