Effects of acute stress on the contents of catecholamines and neuropeptides in chromaffin tissues of the newborn rabbit.
The neuropeptides enkephalin (ENK), galanin (GAL) and neuropeptide Y (NPY) are abundantly expressed in the paraaortic body (PAB) and adrenal glands of the newborn rabbit. To examine whether these neuropeptides are affected by acute stress, we exposed neonatal rabbits to asphyxia, insulin-induced hypoglycemia, and reserpine. Asphyxia, caused by rebreathing for 60 min in an airtight box, reduced the content of catecholamines (CAs) in the adrenal glands and increased ENK-like immunoreactivity (-LI) in the PAB. Insulin-induced hypoglycemia reduced the content of CAs as well as ENK-LI in the adrenal glands. Reserpine caused a marked depletion of the CAs both in the PAB and in the adrenal glands. In contrast, reserpine did not cause any change in the contents of the neuropeptides in either organ. These data indicate that tissue levels of the neuropeptides GAL-LI and NPY-LI, coexisting with CA in the PAB and the adrenal glands, are not biochemically affected by asphyxia, hypoglycemia or reserpine, whereas tissue levels of ENK-LI are reduced by hypoglycemia and, to some extent, are increased by asphyxia. Furthermore, even the CAs in the PAB were unaffected by asphyxia and hypoglycemia. Also, while reserpine reduces CA content, peptide levels are unaffected.
Wikström
L M
LM
Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Rökaeus
A
A
Fried
G
G
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D001237:Asphyxia / Q000503:physiopathology; D001786:Blood Glucose / Q000187:drug effects; D002395:Catecholamines / Q000378:metabolism*; D004745:Enkephalins / Q000378:metabolism; D019004:Galanin / Q000378:metabolism; D007003:Hypoglycemia / Q000139:chemically induced / Q000503:physiopathology; D007328:Insulin / Q000494:pharmacology; D009478:Neuropeptide Y / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism*; D010220:Para-Aortic Bodies / Q000378:metabolism*; D011817:Rabbits; D012110:Reserpine / Q000494:pharmacology; D013312:Stress, Physiological
D001786:Blood Glucose; D002395:Catecholamines; D004745:Enkephalins; D007328:Insulin; D009478:Neuropeptide Y; D009479:Neuropeptides; D019004:Galanin; D012110:Reserpine