Conformational studies of a synthetic peptide from the putative lipid-binding domain of bovine milk component PP3.
In bovine milk, a glycosylated phosphoprotein, component PP3, is known for its remarkable emulsifying properties and its capability to inhibit lipolytic activities. The determination of its primary structure is not sufficient to explain these properties. Secondary structure predictions of component PP3 and of its homologous proteins were achieved using a combination of multiple predictive methods. Based on this study, the f 119-135 region of component PP3 was proposed to be likely to adopt an amphipathic helical conformation, which is a lipid-binding motif. The conformation of the synthetic peptide corresponding to the C-terminal f 119-135 part of bovine component PP3 was analyzed by circular dichroism experiments using various media. The circular dichroism data indicated that the peptide was able to form an amphipathic alpha-helix structure in trifluoroethanol as well as in the presence of sodium dodecyl sulfate or acidic and neutral lipids, but not in water. Moreover, the conformation of this peptide is solvent dependent because it was found to adopt a beta-sheet structure for low concentrations of sodium dodecyl sulfate or a low molar ratio of acidic lipid to peptide. Tensiometric measurements showed that the amphipathic C-terminal region of component PP3 is highly tensioactive and, thus, must be responsible for the particular behavior of the protein in emulsions.
Campagna
S
S
Laboratoire des BioSciences de l'Aliment, Unité associée à l'Institut National de la Recherche Agronomique, Université H. Poincaré-Nancy, France.
Vitoux
B
B
Humbert
G
G
Girardet
J M
JM
Linden
G
G
Haertle
T
T
Gaillard
J L
JL
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D002162:Camelus; D002364:Caseins / Q000737:chemistry*; D002417:Cattle; D002942:Circular Dichroism; D006023:Glycoproteins / Q000737:chemistry*; D050356:Lipid Metabolism; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D051381:Rats; D016415:Sequence Alignment
D002364:Caseins; D006023:Glycoproteins; C085522:PP3 protein, Bos taurus; D010446:Peptide Fragments; D010455:Peptides