title: Wavelength specific activation of PI 3-kinase by UVB irradiation.
abstract: We investigated the effect of UVB irradiation on phospholipid metabolism using normal human diploid skin fibroblasts. When cells were exposed to monochromatic UV light, the intracellular PIP3 level was increased within 1 min in a wavelength-specific manner. The increase was most marked in the UVB range and was inhibited by a PI 3-kinase inhibitor, tyrosine kinase inhibitor, or antioxidant reagents. Furthermore, the decrease of cell viability induced by UVB irradiation was significantly blocked by wortmannin. These results suggest that PI 3-kinase activation is involved in novel cellular responses specific for UVB that lead to cell death.
authors.affiliation: Department of Biomolecular Science, Institute of Biomedical Sciences, Fukushima Medical College, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kabuyama
authors.forename: M
authors.initials: M
authors.lastname: Hamaya
authors.forename: Y
authors.initials: Y
authors.lastname: Homma
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000528:radiation effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species; D014466:Ultraviolet Rays; D000077191:Wortmannin
chemical_list: D000730:Androstadienes; D004791:Enzyme Inhibitors; D019869:Phosphatidylinositol 3-Kinases; D000081082:Phosphoinositide-3 Kinase Inhibitors; D011505:Protein-Tyrosine Kinases; D017382:Reactive Oxygen Species; D000077191:Wortmannin