title: Structure-function studies on beta-glycosidase from Sulfolobus solfataricus. Molecular bases of thermostability.
abstract: beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a thermostable tetrameric protein with a molecular mass of 240 kDa which is stable in the presence of detergents and has a maximal activity above 95 degrees C. An understanding of the structure-function relationship of the enzyme under different chemical-physical conditions is of fundamental importance for both theoretical and application purposes. In this paper we report the effect of basic pH values on the structural stability of this enzyme. The structure of the enzyme was studied at pH 10 and in the temperature range 25-97.5 degrees C using circular dichroism, Fourier-transform infrared and fluorescence spectroscopy. The spectroscopic data indicated that the enzyme stability was strongly affected by pH 10 suggesting that the destabilization of the protein structure is correlated with the perturbation of ionic interactions present in the native protein at neutral pHs. These experiments give support to the observation derived from the 3D-structure, that large ion pair networks on the surface stabilize Sulfolobus solfataricus beta-glycosidase.
authors.affiliation: Istituto di Biochimica delle Proteine ed Enzimologia, Consiglio Nazionale delle Ricerche, Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: D'Auria
authors.forename: M
authors.initials: M
authors.lastname: Moracci
authors.forename: F
authors.initials: F
authors.lastname: Febbraio
authors.forename: F
authors.initials: F
authors.lastname: Tanfani
authors.forename: R
authors.initials: R
authors.lastname: Nucci
authors.forename: M
authors.initials: M
authors.lastname: Rossi
mesh_terms: D002942:Circular Dichroism; D017666:Deuterium Oxide / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008958:Models, Molecular; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D016944:Sulfolobus / Q000201:enzymology*; D013816:Thermodynamics
chemical_list: D006026:Glycoside Hydrolases; D017666:Deuterium Oxide