New mathematical procedure for analysis of data obtained by means of circular dichroism titration method.
A new approach for analysis of circular dichroism titration data for the investigation of drug-protein binding is proposed. The molar ellipticity change and the total drug concentration are considered as a function of the concentration of unbound (free) drug. A new minimization algorithm for estimating the number of classes of binding sites and the association constants is developed. The proposed algorithm offers a statistical criterion to choose the best model for fit of experimentea data. In addition the given procedure estimates the concentration of umbound drug.
Vandev
D
D
University of Sofía, Faculty of Mathematics, Department of Statistics, Sofía, Bulgaria.
Prodanova
K
K
Russeva
V
V
D000465:Algorithms; D001665:Binding Sites; D002942:Circular Dichroism; D008962:Models, Theoretical; D004364:Pharmaceutical Preparations / Q000378:metabolism; D011485:Protein Binding
D004364:Pharmaceutical Preparations