title: In planta localization of a beta-1,4-endoglucanase secreted by Heterodera glycines.
abstract: Polyclonal sera specific to beta-1,4-endoglucanases (cellulases) synthesized in the subventral esophageal gland cells of the soybean cyst nematode, Heterodera glycines, were used to provide the first identification of a nematode esophageal gland protein that is secreted into host plant tissue. Sera generated to proteins encoded by Hg-eng-1 and Hg-eng-2 (endoglucanases) did not cross-react with soybean root proteins on Western blots (immunoblots) or in immunofluorescence microscopy of noninoculated (control) soybean root sections. In cross sections of soybean roots at 24 h after inoculation of roots with second-stage juveniles of H. glycines, HG-ENG-1 was localized within the nematode's subventral gland cells and was not detected in root tissue. HG-ENG-2 was localized within the subventral gland cells and was secreted from the juvenile's stylet into root cortical tissue at 24 h after inoculation of roots with second-stage juveniles of H. glycines. HG-ENG-2 was localized along the juvenile's migratory path through the root cortex.
authors.affiliation: Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616, USA.
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: D
authors.initials: D
authors.lastname: Meyers
authors.forename: Y
authors.initials: Y
authors.lastname: Yan
authors.forename: T
authors.initials: T
authors.lastname: Baum
authors.forename: G
authors.initials: G
authors.lastname: Smant
authors.forename: R
authors.initials: R
authors.lastname: Hussey
authors.forename: E
authors.initials: E
authors.lastname: Davis
mesh_terms: D000818:Animals; D002480:Cellulase / Q000276:immunology / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D009348:Nematoda / Q000201:enzymology* / Q000254:growth & development / Q000472:pathogenicity*; D010944:Plants / Q000201:enzymology* / Q000469:parasitology*; D014018:Tissue Distribution
chemical_list: D002480:Cellulase