[A method of isothermal gene amplification].
A gene amplification technique applied to the detection of HIV and which can be implemented without programmable thermocyclers is described. The reaction products are high molecular weight strands of DNA that can be detected using simple methods, even without electrophoresis. This is a promising technique for in situ amplification experiments. Our findings show that this amplification method is compatible with the temperature levels encountered in living cells. This technology could thus be the basis for a new class of drugs intended for treatment by gene therapy of certain diseases, including malaria and AIDS.
David
F
F
Laboratoire de recherches associatives, Franconville, France.
Turlotte
E
E
D001483:Base Sequence; D016679:Genome, Viral; D006678:HIV / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D021141:Nucleic Acid Amplification Techniques; D013696:Temperature