title: Validated capillary electrophoresis method for the determination of atropine and scopolamine derivatives in pharmaceutical formulations.
abstract: The simultaneous determination of atropine and scopolamine derivatives, which have similar structures, was investigated by using capillary zone electrophoresis. The effects of buffer pH, buffer concentration and hydroxypropyl-beta-cyclodextrin concentration on migration time and resolution of the investigated compounds were systematically studied. The selected electrophoretic buffer consisted of a 80 mM sodium citrate pH 2.5, containing 2.5 mM hydroxypropyl-beta-cyclodextrin as the complexing agent. Quantitative analysis was validated by testing the reproducibility of the method, giving a relative standard deviation less than 1 and 2% for the intermediate precision of migration times and peak area ratios, respectively. The linearity of the method was assessed between 50 and 150% of the theoretical content (coefficient of correlation greater than 0.99). The proposed method was found to be suitable and accurate for the determination of these basic drugs in pharmaceutical preparations.
authors.affiliation: Laboratory of Pharmaceutical Analytical Chemistry, University of Geneva, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Cherkaoui
authors.forename: L
authors.initials: L
authors.lastname: Mateus
authors.forename: P
authors.initials: P
authors.lastname: Christen
authors.forename: J L
authors.initials: JL
authors.lastname: Veuthey
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin; D001286:Atropine Derivatives / Q000032:analysis*; D003505:Cyclodextrins / Q000737:chemistry; D004340:Drug Contamination; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015203:Reproducibility of Results; D012602:Scopolamine Derivatives / Q000032:analysis*; D012680:Sensitivity and Specificity; D013997:Time Factors; D047392:beta-Cyclodextrins
chemical_list: D001286:Atropine Derivatives; D003505:Cyclodextrins; D012602:Scopolamine Derivatives; D047392:beta-Cyclodextrins; D000073738:2-Hydroxypropyl-beta-cyclodextrin