In vitro and in vivo comparison of argon-pumped and diode lasers for photodynamic therapy using second-generation photosensitizers.
OBJECTIVE
Three prototype microchannel-cooled stacked diode array lasers were compared with the currently used conventional argon ion laser-pumped tunable dye lasers for suitability as light sources in photodynamic therapy (PDT) treatment.


METHODS
The PDT response of Chinese hamster ovary (CHO-K1) cells in culture and SMT-F tumor bearing mice treated with chloro-aluminum sulfonated phthalocyanin (CASPc), benzoporphyrin derivative mono-acid (BPD-MA), and lutetium texaphyrin (Lutex) was determined using each laser light source. Survival of the CHO cells was measured using a cloning assay. Tumor regression/eradication was used to assess response in the mice.


RESULTS
Both sources of laser light produced comparable PDT responses in the two systems tested.


CONCLUSIONS
It would be possible to replace the currently used argon ion laser-pumped dye laser systems with the diode lasers tested.
Hammer-Wilson
M J
MJ
Beckman Laser Institute and Medical Clinic, University of California, Irvine 92697-1475, USA.
Sun
C H
CH
Ghahramanlou
M
M
Berns
M W
MW
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D004354:Drug Screening Assays, Antitumor; D005260:Female; D066298:In Vitro Techniques; D007211:Indoles / Q000627:therapeutic use*; D053685:Laser Therapy; D008187:Lutetium / Q000627:therapeutic use*; D008665:Metalloporphyrins / Q000627:therapeutic use*; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy; D009942:Organometallic Compounds / Q000627:therapeutic use*; D010053:Ovary / Q000187:drug effects; D010778:Photochemotherapy / Q000295:instrumentation* / Q000379:methods; D017319:Photosensitizing Agents / Q000627:therapeutic use*; D011166:Porphyrins / Q000627:therapeutic use*
D007211:Indoles; D008665:Metalloporphyrins; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011166:Porphyrins; C102825:motexafin lutetium; C052736:chloroaluminum tetrasulfophthalocyanine; C504224:benzoporphyrin D; D008187:Lutetium; C437683:motexafin gadolinium