[Synthesis of novel fructo-oligosaccharides (FOS) by enzymatic reaction].
Fructo-oligosaccharides (FOS) are new food ingredients that are able to beneficially affect the host by selectively stimulating the growth and/or activity of colonic bifidobacteria (concept of prebiotics). A commercial enzyme preparation was found to possess a high fructosyltransferase activity and could be used as a biocatalyst for the industrial production of FOS from sucrose. Under optimum conditions (pH: 5.5, temperature: 55 degrees C and 7 units of fructosyltransferase activity per gram sucrose), in presence of glucose (competitive inhibitor) the actual yield reached the theoretical value (up to 50%). Actually, FOS that are commercially available for their prebiotic properties belong to inulin type with low degree of polymerisation (DP:3 to 10). Our FOS were identified by both HPLC and 13C-NMR spectrometry as neo-FOS type (neo-kestose, neo-nystose and neo-fructofuranosylnystose), a new structure which is very close to inulin type (same linkage between fructosyl units). The neo-FOS may act as a prebiotic factor due to their structural similarity with inulin type.
Grizard
D
D
Laboratoire de Pharmacognosie et de Biotechnologies, UFR de Pharmacie, Clermont-Ferrand, France.
Barthomeuf
C
C
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005632:Fructose; D006602:Hexosyltransferases / Q000378:metabolism*; D007202:Indicators and Reagents; D007700:Kinetics; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000737:chemistry; D013395:Sucrose; D001426:Bacterial Proteins
D005632:Fructose; D006602:Hexosyltransferases; D007202:Indicators and Reagents; D009844:Oligosaccharides; D013395:Sucrose; C021451:inulosucrase; D001426:Bacterial Proteins