Whole blood capcellia CD4/CD8 immunoassay for enumeration of CD4+ and CD8+ peripheral T lymphocytes.
We evaluated the Whole Blood Capcellia(R) CD4/CD8, an immunoenzymatic method that provides absolute counts of CD4+ and CD8+ T cells in peripheral blood. The assay is based on the separation of T cells by use of an anti-CD2 magnetic bead suspension, followed by reaction of the CD4 or CD8 molecules with the corresponding monoclonal antibody coupled to peroxidase. CD4-positive monocytes were excluded from the assay. Freeze-dried magnetic bead-T-cell complexes were used as calibrators. Capcellia counts from HIV-1-infected patients were compared with those obtained by flow cytometry as the comparison method. The results by Capcellia correlated well with those by flow cytometric analysis: r2 = 0.95; P <0.001; (y = 0.96x - 22.1); Sy|x = 64 for CD4; r2 = 0.81; P <0.001; (y = 1.26x - 76.4); Sy|x = 139 for CD8; n = 76. The correlation between CD4+ T-cell counts determined by two trained experimenters was significant (r2 = 0.96). Our results indicate that this new ELISA technique for lymphocyte immunophenotyping is an efficient alternative to flow cytometry.
Carrière
D
D
Ligne de Recherche en Immunologie, Sanofi Recherche, 371 rue du professeur Joseph Blayac, 34184 Montpellier Cedex 04, France. dominique.carriere@sanofi.com
Vendrell
J P
JP
Fontaine
C
C
Jansen
A
A
Reynes
J
J
Pagès
I
I
Holzmann
C
C
Laprade
M
M
Pau
B
B
D000911:Antibodies, Monoclonal; D016516:CD4-CD8 Ratio / Q000379:methods*; D002138:Calibration; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry; D006679:HIV Seropositivity / Q000097:blood; D015497:HIV-1 / Q000276:immunology; D006801:Humans; D008280:Magnetics; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D011933:Reagent Kits, Diagnostic