title: Expression of ACTH receptors (MC2-R and MC5-R) in the glomerulosa and the fasciculata-reticularis zones of bovine adrenal cortex.
abstract: The recent cloning of a family of melanocortin receptors (MC-R) has identified five distinct G protein- and adenylate cyclase-coupled receptors. The MC2-receptor (MC2-R) preferentially binds ACTH. It is expressed in the adrenal cortex and is hence considered to be the ACTH receptor. The MC5-receptor (MC5-R) binds ACTH and alpha-MSH and is more widely expressed. The aim of this work was to study the sites of MC5-R expression in the bovine adrenal cortex and to compare the regulation of the expression of MC2-R and MC5-R in bovine adrenocortical cells in primary culture. Analysis of the expression of MC5-R was obtained by RT-PCR, using total RNA purified from glomerulosa and fasciculata zones of bovine adrenocortical tissue. MC5-R expression could be detected in RNA from the glomerulosa zone but was undetectable in the fasciculata zone. In bovine adrenocortical cells in culture, ACTH stimulates MC5-R expression in the glomerulosa and fasciculata cells. A DNA fragment, was obtained using primers based on the bovine ACTH receptor (MC2-R) sequence. This fragment was detected in RNA from the two zones. The probe was used to quantify MC2-R by Ribonuclease Protection assay and we observed that MC2-R mRNA is 3.6-fold more abundant in glomerulosa than in fasciculata-reticularis cells.
authors.affiliation: CEA, INSERM U. 244, DBMS, Grenoble, France.
authors.forename: P
authors.initials: P
authors.lastname: Liakos
authors.forename: E M
authors.initials: EM
authors.lastname: Chambaz
authors.forename: J J
authors.initials: JJ
authors.lastname: Feige
authors.forename: G
authors.initials: G
authors.lastname: Defaye
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D017403:In Situ Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D018043:Receptors, Corticotropin / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012260:Ribonucleases; D014018:Tissue Distribution; D015383:Zona Fasciculata / Q000166:cytology / Q000378:metabolism*; D015384:Zona Glomerulosa / Q000166:cytology / Q000378:metabolism*; D015385:Zona Reticularis / Q000166:cytology / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018043:Receptors, Corticotropin; D000324:Adrenocorticotropic Hormone; D012260:Ribonucleases