Search for genes responsible for UV susceptibility of human cells: involvement of syndecan-1 in UV resistance.
UVr-1 UV-resistant cells were established from UV-sensitive human RSa cells. We looked for genes expressed differentially between UVr-1 and RSa cells using PCR-based mRNA differential display to elucidate the molecular mechanisms underlying UV resistance. The transcription levels of syndecan-1 mRNA were increased in UVr-1 cells compared with those of RSa cells. Syndecan-1 is a transmembrane heparan sulfate proteoglycan and associates with cell adhesion and the cytoskeleton. Flow cytometric analysis using anti-syndecan-1 monoclonal antibody revealed that syndecan-1 was more abundant in UVr-1 cells than in RSa cells. The MTT method revealed that UVr-1 cells treated with the antibody showed higher sensitivity to UV cell killing than mock-treated cells. Studies using antisense oligonucleotides for syndecan-1 showed that antisense-treated UVr-1 cells became sensitive to UV cell killing. Thus, syndecan-1 might be involved in UV resistance in UVr-1 cells.
Kikuno
K
K
School of Medicine, Chiba University, Japan.
Kita
K
K
Nomura
J
J
Hiwasa
T
T
Yonemitsu
H
H
Suzuki
N
N
D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015152:Blotting, Northern; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D005434:Flow Cytometry; D005796:Genes / Q000528:radiation effects*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000276:immunology / Q000502:physiology / Q000528:radiation effects*; D011509:Proteoglycans / Q000235:genetics* / Q000276:immunology / Q000502:physiology / Q000528:radiation effects*; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D053668:Syndecan-1; D053667:Syndecans; D014466:Ultraviolet Rays
D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D011509:Proteoglycans; D012333:RNA, Messenger; C508241:SDC1 protein, human; D053668:Syndecan-1; D053667:Syndecans