title: Isolation and characterization of the gene coding for human cytidine deaminase.
abstract: The human gene coding for cytidine deaminase (CD), the enzyme which catalyzes the deamination of cytidine and deoxycytidine to uridine and deoxyuridine, was isolated and structurally characterized. CD is a single copy gene with a length of 31 kb and consists of four exons. Exon-intron junctions do not bracket functional domains of the encoded protein as the boundary between exons 2 and 3 interrupts the catalytically important zinc-finger domain, which is well conserved along phylogenesis. 5'-RACE and RNase mapping experiments identify one major and multiple other minor transcription initiation sites, which are present in placenta as well as in the myeloid cell lines, HL-60 and U937. The 5'-flanking region of the gene contains an orientation-dependent functional promoter and is characterized by the presence of several potential sites for the binding of known transcriptional factors.
authors.affiliation: Laboratory of Molecular Biology, Centro Catullo e Daniela Borgomainerio, Istituto di Ricerche Farmacologiche 'Mario Negri', via Eritrea, 62, 20157 Milan, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Demontis
authors.forename: M
authors.initials: M
authors.lastname: Terao
authors.forename: M
authors.initials: M
authors.lastname: Brivio
authors.forename: S
authors.initials: S
authors.lastname: Zanotta
authors.forename: M
authors.initials: M
authors.lastname: Bruschi
authors.forename: E
authors.initials: E
authors.lastname: Garattini
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells / Q000166:cytology / Q000378:metabolism; D003564:Cytidine Deaminase / Q000235:genetics*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D005091:Exons; D015972:Gene Expression Regulation, Neoplastic; D005796:Genes / Q000235:genetics*; D018922:HL-60 Cells / Q000166:cytology / Q000378:metabolism; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors; D014158:Transcription, Genetic; D014407:Tumor Cells, Cultured / Q000166:cytology / Q000378:metabolism; D020298:U937 Cells
chemical_list: D014157:Transcription Factors; D004247:DNA; D003564:Cytidine Deaminase