IgG immune response to B19 parvovirus VP1 and VP2 linear epitopes by immunoblot assay.
Human B19 parvovirus recombinant capsid proteins VP1 and VP2 were expressed in E. coli and purified. Recombinant proteins were used to detect a specific IgG immune response against VP1 and VP2 linear epitopes by immunoblot assay. A total of 222 serum samples from 218 apparently immunocompetent subjects with different clinical conditions and laboratory evaluations with regards to B19 infection were analyzed. The sera had previously been tested for B19 DNA and for specific IgM and IgG against VP2 conformational antigens by ELISA assay. The data show that, during the active or very recent phase of infection, IgG anti-VP1 linear epitopes appear in concomitance and with the same frequency as IgG anti-VP2 conformational antigens. IgG against conformational VP2 antigens and against linear VP1 epitopes seem to persist for months or years in the majority of individuals. IgG against VP2 linear epitopes are generally present during the active or very recent phase of infection and during the convalescent phase, while they are present only in about 20% of subjects with signs of a past B19 infection.
Manaresi
E
E
Department of Clinical and Experimental Medicine, University of Bologna, Italy.
Gallinella
G
G
Zerbini
M
M
Venturoli
S
S
Gentilomi
G
G
Musiani
M
M
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D002213:Capsid / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D036022:Capsid Proteins; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000097:blood; D000939:Epitopes / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000276:immunology; D011247:Pregnancy; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D036022:Capsid Proteins; D004279:DNA, Viral; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins; C095547:capsid protein VP1, parvovirus B19; C095548:capsid protein VP2, parvovirus B19