title: The in vivo effects of bisGMA on murine uterine weight, nucleic acids and collagen.
abstract: The aim of this investigation was to evaluate the ability of commercial bisGMA to stimulate growth in an estrogen-sensitive target tissue. Adult, female, Swiss-Webster mice were ovariectomized and received either oil, estradiol (100 microg/kg), or one of two bisGMA doses (25 microg/kg or 100 microg/kg). Starting on the day of surgery, the hormone, drug or oil was injected subcutaneously 3 times a week. After 3 wk of treatment, the animals were sacrificed, the uteri removed, weighed and stored at -80 degrees C for biochemical analysis. The uteri from ovariectomized mice receiving high dose (100 microg/kg) bisGMA or estradiol showed a significant increase in normalized wet weight that was 29% and 786%, respectively, greater than the ovariectomized control uterine normalized weights. In the low bisGMA dose (25 microg/kg) group, normalized uterine wet weights were not statistically significant from ovariectomized controls. Biochemical analyses of uterine tissues revealed that estradiol resulted in maintaining DNA content, RNA content, RNA/DNA ratios and collagen content significantly above the ovariectomized control. Neither the low nor high doses of bisGMA stimulated RNA content, DNA content or RNA/DNA ratios above ovariectomized controls. However, the high dose (100 microg/kg) of bisGMA caused a significant increase above ovariectomized controls in uterine collagen content.
authors.affiliation: Section of Periodontology, College of Dentistry, The Ohio State University, Columbus, USA. Mariotti.3@osu.edu
authors.forename: A
authors.initials: A
authors.lastname: Mariotti
authors.forename: K J
authors.initials: KJ
authors.lastname: Söderholm
authors.forename: S
authors.initials: S
authors.lastname: Johnson
mesh_terms: D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate / Q000008:administration & dosage / Q000494:pharmacology*; D003094:Collagen / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D051379:Mice; D009929:Organ Size / Q000187:drug effects; D010052:Ovariectomy; D012313:RNA / Q000096:biosynthesis; D014599:Uterus / Q000187:drug effects*
chemical_list: D017438:Bisphenol A-Glycidyl Methacrylate; D004958:Estradiol; D012313:RNA; D003094:Collagen; D004247:DNA