Photoinduction of arginine decarboxylase activity in leaves of Pharbitis nil.
The activity of arginine decarboxylase (ADC) in leaves of Pharbitis nil was induced by light. The ADC activity increased to a maximum 1 h after illumination, followed by a gradual decrease. This suggested light either induced synthesis of ADC protein de novo or was involved in its activation. Cycloheximide inhibited the photoinduction of ADC activity, and the half life of ADC in leaves was 30-40 min. The temperature and relative humidity in darkness before illumination had no effect on the photoinduction of ADC activity, contrary to the photoresponse of S-adenosylmethionine decarboxylase (SAMDC) activity where the conditions of darkness before lights-on have a marked effect. The light response of the polyamine (PA)-biosynthetic enzyme activity produced transient accumulation of PA. The level of spermidine increased in leaves in which activities of both SAMDC and ADC increased after illumination, while the level of putrescine increased in leaves in which the activity of ADC increased but that of SAMDC did not.
Yoshida
I
I
Department of Biology, Faculty of Science, Osaka City University, Japan.
Hirasawa
E
E
D012437:Adenosylmethionine Decarboxylase / Q000096:biosynthesis; D002262:Carboxy-Lyases / Q000096:biosynthesis*; D004790:Enzyme Induction; D008027:Light; D018515:Plant Leaves / Q000201:enzymology / Q000528:radiation effects; D010944:Plants / Q000201:enzymology / Q000528:radiation effects*
D002262:Carboxy-Lyases; C019722:arginine decarboxylase; D012437:Adenosylmethionine Decarboxylase