Application of new analytical technology to the production of a "well-characterized biological".
The role of new analytical technology in the development of the concept of a "Well Characterized Biological" is to provide suitable methodology that allows the characterization of even the most complex protein sample so that a consistent manufacturing process can be established. Glycoproteins are among the most challenging of products to characterize because of extreme sample microheterogeneity due to the carbohydrate moieties. As an example of the appropriate use of new analytical technology this review will examine the steps necessary to demonstrate that a glycoprotein is a "Well Characterized Biological". The key to characterization of complex protein samples lies in the use of appropriate combinations of the different methods that analyse the sample from substantially orthogonal and independent directions. An important advantage of capillary electrophoresis (HPCE) in this application is the complementarily of the technique with reversed phase HPLC (RPLC). Thus mixtures of variants of a polypeptide that are difficult to separate by RPLC can often be readily resolved by HPCE. Both separation techniques are well suited to the analysis of peptide maps, although RPLC is particularly powerful when used in combination with on-line electrospray mass spectrometry (ESI-MS) which allows for the effective ionization and detection of even high MW glycopeptides. In this sense the ESI-MS is an ideal detector for on-line mass detection after a RPLC separation of medium MW fragments (300 to 6000 emu) that are typically present in an enzyme digest of a protein. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOFMS) is a valuable technique for off-line characterization of CE fractions due to the high sensitivity of the method and its tolerance of samples with moderate levels of salt. The development of an effective protocol for the analysis of glycoform populations of intact glycoproteins by a combination of HPCE and off-line MALDI-TOFMS wil be demonstrated by the successful analysis of two highly heterogeneous glycoproteins, ovalbumin and Desmodus Salivary Plasminogen Activator (DSPA).
Apffel
A
A
Hewlett-Packard Laboratories, Palo Alto, CA 94304, USA.
Chakel
J
J
Udiavar
S
S
Swedberg
S
S
Hancock
W S
WS
Souders
C
C
Pungor
E
E
D000818:Animals; D001688:Biological Products / Q000032:analysis* / Q000096:biosynthesis* / Q000592:standards; D001709:Biotechnology; D002623:Chemistry Techniques, Analytical; D002851:Chromatography, High Pressure Liquid; D019075:Electrophoresis, Capillary; D006023:Glycoproteins / Q000032:analysis; D013058:Mass Spectrometry; D010047:Ovalbumin / Q000032:analysis; D010449:Peptide Mapping; D010960:Plasminogen Activators / Q000032:analysis; D011994:Recombinant Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001688:Biological Products; D006023:Glycoproteins; D011994:Recombinant Proteins; C069588:salivary plasminogen activator alpha 1, Desmodus rotundus; D010047:Ovalbumin; D010960:Plasminogen Activators