Natural interferon enhances expression of placental alkaline phosphatase in human seminoma xenograft.
The purpose of this study was to investigate the effect of natural interferon (IFN) on the expression of placental alkaline phosphatase (PLAP) in a human seminoma xenograft in severe combined immunodeficient mice. Mice were injected intramuscularly with 3x10(5) U/mouse of IFN, twice a day, for five consecutive days. A significant increase in PLAP level of the xenografts followed IFN treatment. A radiolabeled anti-PLAP monoclonal antibody (MAb) was intravenously injected on the first day of IFN administration in order to determine if IFN has the potential to enhance the efficacy of an anti-PLAP MAb in the detection of seminoma. Enhanced retention of the anti-PLAP MAb was observed at 7 and 11 days after MAb administration. Thus, IFN treatment appears to have some effect on the efficacy of the anti-PLAP MAb in the detection of seminoma xenografts.
Imao
T
T
Department of Urology, School of Medicine, Kanazawa University, Japan.
Koshida
K
K
Konaka
H
H
Uchibayashi
T
T
Yokoyama
K
K
Hirano
K
K
Namiki
M
M
D000469:Alkaline Phosphatase / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D018239:Seminoma / Q000000981:diagnostic imaging / Q000201:enzymology*; D013736:Testicular Neoplasms / Q000000981:diagnostic imaging / Q000201:enzymology*; D013737:Testis / Q000201:enzymology; D014183:Transplantation, Heterologous
D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D007372:Interferons; D000469:Alkaline Phosphatase