Baculovirus expression of chimeric hepatitis B virus core particles with hepatitis E virus epitopes and their use in a hepatitis E immunoassay.
Two hepatitis B core proteins bearing the immunodominant region of the hepatitis E virus (HEV) capsid protein, one at the C terminus of hepatitis B virus core antigen (HBcAg) and the other within the HBcAg immunodominant loop, were constructed. Both chimeric proteins exhibited HEV reactivity, but only the first construct retained HBcAg reactivity. The second construct was used to develop an anti-HEV test which is equivalent to a commercial test for the detection of anti-HEV immunoglobulin G (IgG) but is more sensitive for the detection of anti-HEV IgM.
Touze
A
A
Institut de Virologie de Tours and Laboratoire des Processus Infectieux et Tumoraux, Faculté des Sciences Pharmaceutiques "Philippe Maupas," 37200 Tours, France.
Enogat
N
N
Buisson
Y
Y
Coursaget
P
P
D016367:Baculoviridae / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006508:Hepatitis Antibodies / Q000097:blood*; D018963:Hepatitis Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology; D019694:Hepatitis B, Chronic; D016751:Hepatitis E / Q000175:diagnosis / Q000276:immunology; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology; D012680:Sensitivity and Specificity
D006508:Hepatitis Antibodies; D018963:Hepatitis Antigens; D006512:Hepatitis B Core Antigens; D016056:Immunodominant Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011993:Recombinant Fusion Proteins