[Alterations in the content of collagenous constituents of amniotic fluid in the course of EPH-gestosis].
OBJECTIVE
EPH-gestosis may cause placental insufficiency and affect the metabolism of placenta and fetus. Various metabolites of amniotic fluid may reflect the fetal metabolism.


METHODS
It was decided to compare the content and some biochemical features of hydroxyproline-containing collagenous constituents in the amniotic fluid derived from normal gestations and gestations affected by EPH-gestosis.


METHODS
Amniotic fluid was taken during labour (full-term gestations) by amniocentesis from 10 healthy women (control group) and 10 women with EPH-gestosis. UV-absorption spectra, protein and hydroxyproline were measured in non-dialyzed and dialyzed amniotic fluid. Hydroxyproline-containing collagenous constituents of amniotic fluid were chromatographed on Sephadex G-200.


RESULTS
Statistical differences in concentrations of total and non-dialyzable hydroxyproline between normal and gestotic amniotic fluid were not found. In contrast to that, gestotic amniotic fluid contains more dialyzable hydroxyproline (6.2 mg/ml) than normal fluid (4.5 mg/ml; p < 0.05). Molecular sieving on Sephadex G-200 shows that this difference is due to higher content of low molecular collagen degradation products.


CONCLUSIONS
Amniotic fluid, derived from pregnancies complicated by EPH-gestosis, contains higher amount of low molecular collagen degradation products than normal amniotic fluid. It is probably the result of more intensive degradation of fetal and placental collagen.
Gogiel
T
T
Zakładu Biochemii Akademii Medycznej w Białymstoku.
Bielecki
D A
DA
Bańkowski
E
E
D000653:Amniotic Fluid / Q000737:chemistry*; D003094:Collagen / Q000032:analysis*; D005260:Female; D006801:Humans; D011225:Pre-Eclampsia / Q000175:diagnosis*; D011247:Pregnancy
D003094:Collagen