SERE, a widely dispersed bacterial repetitive DNA element.
The presence of a Salmonella serotype Enteritidis repeat element (SERE) located within the upstream regulatory region of the sefABCD operon encoding fimbrial proteins is reported. DNA dot-blot hybridisation analyses and computerised searches of genetic databases indicate that SERE is well conserved and widely distributed throughout the bacterial and archaeal kingdoms. A SERE-based polymerase chain reaction (SERE-PCR) assay was developed to fingerprint 54 isolates of Enteritidis representing nine distinct phage types and 54 isolates of other Salmonella serotypes. SERE-PCR identified five distinct fingerprint profiles among the 54 Enteritidis isolates; no correlation between phage types and SERE-PCR fingerprint patterns was noticed. SERE-PCR was reproducible, rapid and easy to perform. The results of this investigation suggest that the limited heterogeneity of SERE-PCR fingerprint patterns can be utilised to develop serotype- and serogroup-specific fingerprint patterns for isolates of Enteritidis.
Rajashekara
G
G
Department of Veterinary PathoBiology, University of Minnesota, St Paul 55108, USA.
Koeuth
T
T
Nevile
S
S
Back
A
A
Nagaraja
K V
KV
Lupski
J R
JR
Kapur
V
V
D000818:Animals; D001483:Base Sequence; D016384:Consensus Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D020071:Interspersed Repetitive Sequences; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009876:Operon; D016133:Polymerase Chain Reaction / Q000379:methods* / Q000706:statistics & numerical data; D015203:Reproducibility of Results; D012483:Salmonella Phages / Q000145:classification / Q000235:genetics; D012477:Salmonella enteritidis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012689:Sequence Homology, Nucleic Acid; D012703:Serotyping
D017931:DNA Primers; D004269:DNA, Bacterial