Brainstem neurons with descending projections to the spinal cord of two elasmobranch fishes: thornback guitarfish, Platyrhinoidis triseriata, and horn shark, Heterodontus francisci.
We studied two cartilaginous fishes and described their brainstem supraspinal projections because most nuclei in the reticular formation can be identified that way. A retrogradely transported tracer, horseradish peroxidase or Fluoro-Gold, was injected into the spinal cord of Platyrhinoidis triseriata (thornback guitarfish) or Heterodontus fransisci (horn shark). We described labeled reticular cells by their position, morpohology, somatic orientation, dendritic processes, and laterality of spinal projections. Nineteen reticular nuclei have spinal projections: reticularis (r.) dorsalis, r. ventralis pars alpha and beta, r. gigantocellularis, r. magnocellularis, r. parvocellularis, r. paragigantocellularis lateralis and dorsalis, r. pontis caudalis pars alpha and beta, r. pontis oralis pars medialis and lateralis, r. subcuneiformis, r. peduncularis pars compacta, r. subcoeruleus pars alpha, raphe obscurus, raphe pallidus, raphe magnus, and locus coeruleus. Twenty nonreticular nuclei have spinal projections: descending trigeminal, retroambiguus, solitarius, posterior octaval, descending octaval, magnocellular octaval, ruber, Edinger-Westphal, nucleus of the medial longitudinal fasciculus, interstitial nucleus of Cajal, latral mesencephalic complex, periventricularis pretectalis pars dorsalis, central pretectal, ventromedial thalamic, posterior central thalamic, posterior dorsal thalamic, the posterior tuberculum, and nuclei B, F, and J. The large number of distinct reticular nuclei with spinal projections corroborates the hypothesis that the reticular formation of elasmobranches is complexly organized into many of the same nuclei that are found in frogs, reptiles, birds, and mammals.
Cruce
W L
WL
Neurobiology Department, Northeastern Ohio Universities College of Medicine, Rootstown 44272, USA. wlrc@neoucom.edu
Stuesse
S L
SL
Northcutt
R G
RG
D000818:Animals; D001370:Axonal Transport; D001933:Brain Stem / Q000033:anatomy & histology* / Q000502:physiology; D004027:Diencephalon / Q000033:anatomy & histology / Q000502:physiology; D005399:Fishes / Q000033:anatomy & histology*; D005456:Fluorescent Dyes; D006735:Horseradish Peroxidase; D008636:Mesencephalon / Q000033:anatomy & histology / Q000502:physiology; D009434:Neural Pathways / Q000033:anatomy & histology / Q000502:physiology; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D011149:Pons / Q000033:anatomy & histology / Q000502:physiology; D012154:Reticular Formation / Q000033:anatomy & histology / Q000502:physiology; D013045:Species Specificity; D013116:Spinal Cord / Q000033:anatomy & histology* / Q000502:physiology; D013266:Stilbamidines
C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D005456:Fluorescent Dyes; D013266:Stilbamidines; D006735:Horseradish Peroxidase