title: Lentil lectin enriched microsomes from the plasma membrane of the human B-lymphocyte cell line H2LCL carry a heavy load of type-1 porin.
abstract: Using an established biochemical approach, five subcellular fractions of human B lymphocytes were prepared by differential centrifugation. Crude membranes were passed over a lentil lectin column to enrich carbohydrate-coated cell surface microsomes. The lectin-bound fraction contained a high amount of plasma membrane-derived microsomes as indicated by cell surface markers. All subcellular fractions in Western blots proved to contain distinct but variable amounts of porin. There was a strong increase in porin content from crude membranes to plasma membrane-derived vesicles. The porin content of this fraction appeared to be higher than that of mitochondria. In the final step the plasma membrane-derived microsome fraction proved to be devoid of contamination by outer mitochondrial membranes, as revealed by antibodies against the established markers MAO B and Tom20 applied in Western blots. These data prove the extramitochondrial expression of human type-1 porin/ type-1 VDAC.
authors.affiliation: Max-Planck-Institute for Experimental Medicine, Department of Immunochemistry, Göttingen, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Eben-Brunnen
authors.forename: S
authors.initials: S
authors.lastname: Reymann
authors.forename: L A
authors.initials: LA
authors.lastname: Awni
authors.forename: T
authors.initials: T
authors.lastname: Cole
authors.forename: T
authors.initials: T
authors.lastname: Hellmann
authors.forename: K P
authors.initials: KP
authors.lastname: Hellmann
authors.forename: G
authors.initials: G
authors.lastname: Paetzold
authors.forename: J
authors.initials: J
authors.lastname: Kleineke
authors.forename: F P
authors.initials: FP
authors.lastname: Thinnes
authors.forename: H
authors.initials: H
authors.lastname: Götz
authors.forename: N
authors.initials: N
authors.lastname: Hilschmann
mesh_terms: D001402:B-Lymphocytes / Q000378:metabolism*; D015153:Blotting, Western; D002451:Cell Compartmentation; D002461:Cell Line, Transformed; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D037102:Lectins / Q000008:administration & dosage*; D008861:Microsomes / Q000378:metabolism*; D037121:Plant Lectins; D018272:Porins / Q000378:metabolism*
chemical_list: D037102:Lectins; D037121:Plant Lectins; D018272:Porins; C033619:lentil lectin