Spatio-temporal activities in the antennal lobe analyzed by an optical recording method in the male silkworm moth Bombyx mori.
Optical recordings with a voltage-sensitive dye showed that the spatio-temporal pattern of depolarizing responses evoked by electrical stimulation of antennal nerve (AN) was non-homologously distributed in the antennal lobe (AL) of the male silkworm moth, Bombyx mori. Time courses of postsynaptic activities and GABAergic inhibitory potentials of AL neurons were individually demonstrated by pharmacological experiments, i.e. Ca2+ free and bicuculline conditions. GABAergic inhibitory potentials began with a ca. 3 ms delay from the beginning of the postsynaptic activities. Intensity of the postsynaptic activities and GABAergic inhibitory potentials were non-homologously distributed in the AL. Relatively strong postsynaptic activities and GABAergic inhibitory potentials were consistently observed in some parts of the macroglomerular complex (MGC) and/or in some ordinary glomeruli (Gs) in the medial and ventral part of the AL.
Ai
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Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Okada
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K
Hill
E S
ES
Kanzaki
R
R
D000818:Animals; D001640:Bicuculline / Q000494:pharmacology; D012831:Bombyx / Q000502:physiology*; D004558:Electric Stimulation; D005071:Evoked Potentials / Q000502:physiology; D008297:Male; D009474:Neurons / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D009833:Olfactory Pathways / Q000033:anatomy & histology / Q000166:cytology / Q000502:physiology*; D055095:Optics and Photonics; D012679:Sense Organs / Q000294:innervation*; D013569:Synapses / Q000502:physiology; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D005680:gamma-Aminobutyric Acid; D001640:Bicuculline