title: Trypanosoma cruzi genotypes associated with domestic Triatoma sordida in Bolivia.
abstract: Triatoma sordida is the second species of Triatominae considered of epidemiological significance in Bolivia. Associated with Triatoma infestans in various regions, it is as yet the only triatomine species established in human dwellings in localities of Velasco province, Department of Santa Cruz. This domestication is considered as primary. Flagellate parasites were detected in 16.2% of domiciliary T. sordida and the kDNA-PCR confirmed the presence of Trypanosoma cruzi. Frequencies of T. cruzi clonets 20 and 39, common clonets in Bolivian domestic cycle (T. infestans), were established by their direct detection in feces using PCR and hybridization. These clonets present low frequencies in T. sordida and synanthropic mammals. Forty-six stocks were isolated and analysed by multilocus enzyme electrophoresis (MLEE). The MLEE showed a higher clonal diversity than in T. infestans domestic cycle and the genotypes were clustered in the two principal lineages of T. cruzi. Within each lineage, a broad variability was observed. Mixture of genotypes was mostly observed in mammals. The large diversity of T. cruzi in this cycle should be related to its sylvatic origin. Moreover, the current limited sample of stocks suggests a lineage association with specific hosts.
authors.affiliation: UMR CNRS/ORSTOM no 9926, Montpellier, France.
authors.forename: S F
authors.initials: SF
authors.lastname: Brenière
authors.forename: W
authors.initials: W
authors.lastname: Morochi
authors.forename: M F
authors.initials: MF
authors.lastname: Bosseno
authors.forename: J
authors.initials: J
authors.lastname: Ordoñez
authors.forename: T
authors.initials: T
authors.lastname: Gutierrez
authors.forename: F
authors.initials: F
authors.lastname: Vargas
authors.forename: N
authors.initials: N
authors.lastname: Yaksic
authors.forename: F
authors.initials: F
authors.lastname: Noireau
mesh_terms: D000818:Animals; D001838:Bolivia; D002415:Cats; D004285:Dogs; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D051381:Rats; D014224:Triatoma / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000235:genetics* / Q000302:isolation & purification