Generation of carboxy-terminally deleted forms of STAT5 during preparation of cell extracts.
Carboxy-terminally deleted forms of STAT5 have been described to be generated in vivo either by proteolytic processing or by differential splicing mechanisms. By comparing two different cell extraction procedures, we can show that in the mammary gland carboxy-terminally deleted forms are produced in vitro and are not detectable in extracts prepared by SDS lysis.
Garimorth
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K
Institut für Medizinische Chemie und Biochemie, Universität Innsbruck, Fritz Pregl-Strasse 3, Innsbruck, A-6020, Austria.
Welte
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Doppler
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D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D016477:Artifacts; D015153:Blotting, Western; D001943:Breast Neoplasms / Q000737:chemistry; D002457:Cell Extracts / Q000737:chemistry*; D002458:Cell Fractionation / Q000379:methods*; D004268:DNA-Binding Proteins / Q000032:analysis / Q000737:chemistry*; D010450:Endopeptidases / Q000378:metabolism; D005260:Female; D006358:Hot Temperature; D008321:Mammary Glands, Animal / Q000737:chemistry; D051379:Mice; D008894:Milk Proteins; D008969:Molecular Sequence Data; D011233:Precipitin Tests; D011247:Pregnancy; D020033:Protein Isoforms / Q000032:analysis / Q000737:chemistry; D012313:RNA / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D050799:STAT5 Transcription Factor; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D015534:Trans-Activators / Q000032:analysis / Q000737:chemistry*
D002457:Cell Extracts; D004268:DNA-Binding Proteins; D008894:Milk Proteins; D020033:Protein Isoforms; D050799:STAT5 Transcription Factor; D015534:Trans-Activators; D012967:Sodium Dodecyl Sulfate; D012313:RNA; D010450:Endopeptidases