title: The THCRE2 site in the rat tyrosine hydroxylase gene promoter is responsive to phorbol ester.
abstract: Recently, our laboratory has identified a tyrosine hydroxylase (TH) gene promoter element, THCRE2 (located at -97 to -90), that is required for maximal response to cyclic AMP. In this study we test whether the THCRE2 is responsive to phorbol ester. Rat PC12 cells were transfected with a reporter gene construct, TH(-272/+27)-CAT, which is driven by the first 272 bp of the rat TH gene 5' flanking region. Treatment of transfected cells with 0.1 microM 12-O-tetradecanoylphorbol 13-acetate (TPA) elicited a 5-6-fold increase in TH gene proximal promoter activity. Mutagenesis of the THCRE2 sequence diminished TPA-responsiveness of the TH gene promoter by approximately 50%. Minimal promoter constructs driven by a single copy of the region of the TH gene that encodes the THCRE2 (from -117 to -59) were also responsive to TPA. Our results suggest that the THCRE2 is a phorbol ester-responsive element, as well as a cyclic AMP-responsive element.
authors.affiliation: Center for Oral Biology and Eastman Department of Dentistry, University of Rochester Medical Center, NY 14642, USA.
authors.forename: J A
authors.initials: JA
authors.lastname: Best
authors.forename: A W
authors.initials: AW
authors.lastname: Tank
mesh_terms: D000818:Animals; D016297:Mutagenesis, Site-Directed; D016716:PC12 Cells; D011401:Promoter Regions, Genetic; D051381:Rats; D020218:Response Elements; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics*
chemical_list: D014446:Tyrosine 3-Monooxygenase; D013755:Tetradecanoylphorbol Acetate