title: Physical and functional interactions between the herpes simplex virus UL15 and UL28 DNA cleavage and packaging proteins.
abstract: Herpes simplex virus (HSV) DNA is cleaved from concatemers and packaged into capsids in infected cell nuclei. This process requires seven viral proteins, including UL15 and UL28. UL15 expressed alone displays a nuclear localization, while UL28 remains cytoplasmic. Coexpression with UL15 enables UL28 to enter nuclei, suggesting an interaction between the two proteins. Additionally, UL28 copurified with UL15 from HSV-infected cells after ion-exchange and DNA affinity chromatography, and the complex sedimented as a 1:1 heterodimer upon sucrose gradient centrifugation. These findings are evidence of a physical interaction of UL15 and UL28 and a functional role for UL15 in directing UL28 to the nucleus.
authors.affiliation: Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
authors.forename: K M
authors.initials: KM
authors.lastname: Koslowski
authors.forename: P R
authors.initials: PR
authors.lastname: Shaver
authors.forename: J T
authors.initials: JT
authors.lastname: Casey
authors.forename: T
authors.initials: T
authors.lastname: Wilson
authors.forename: G
authors.initials: G
authors.lastname: Yamanaka
authors.forename: A K
authors.initials: AK
authors.lastname: Sheaffer
authors.forename: D J
authors.initials: DJ
authors.lastname: Tenney
authors.forename: N E
authors.initials: NE
authors.lastname: Pederson
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D004279:DNA, Viral / Q000378:metabolism*; D018259:Herpesvirus 1, Human / Q000378:metabolism* / Q000502:physiology; D006801:Humans; D014709:Vero Cells; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D019065:Virus Assembly
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; C069185:DNA cleavage and packaging proteins, Herpesvirus