Drinking and Fos-immunoreactivity in rat brain induced by local injection of angiotensin I into the subfornical organ.
Previous studies suggested that angiotensinergic stimulation in the subfornical organ (SFO) has effects on the anterior third ventricle (AV3V) region and the hypothalamus for dipsogenic response and vasopressin release. In this study, Angiotensin I (ANG I) was directly injected into the SFO and this stimulated drinking. Injection of ANG I into the SFO also induced Fos-immunoreactivity (Fos-ir) in the AV3V region and in the vasopressin neurons of the supraoptic and paraventricular nuclei (SON and PVN). Pretreatment of the SFO with either captopril, an ANG converting enzyme inhibitor, or losartan, an AT1 receptor antagonist, abolished both drinking and Fos-ir induced by ANG I. Water intake partially decreased ANG I-induced Fos-ir in the SON and PVN, but not in the other areas. These results indicate that there is an ANG converting system in the SFO and suggest that neurons in the AV3V region and vasopressin cells in the hypothalamus can be regulated by angiotensinergic components in the SFO.
Xu
Z
Z
Departments of Psychology, University of Iowa, Iowa City, IA 52242, USA.
Xinghong
J
J
D000704:Analysis of Variance; D000803:Angiotensin I / Q000494:pharmacology*; D000818:Animals; D004327:Drinking Behavior / Q000502:physiology*; D007031:Hypothalamus / Q000187:drug effects; D007150:Immunohistochemistry; D008297:Male; D008845:Microinjections; D009474:Neurons / Q000187:drug effects; D016548:Prosencephalon / Q000187:drug effects; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013356:Subfornical Organ / Q000737:chemistry / Q000166:cytology / Q000187:drug effects*
D016760:Proto-Oncogene Proteins c-fos; D000803:Angiotensin I