Secretory pathways in Salmonella Typhimurium-induced fluid accumulation in the porcine small intestine.
The involvement of 5-hydroxytryptamine (5-HT) and 5-HT3 receptors and prostaglandin E2 (PGE2) in Salmonella Typhimurium-induced fluid accumulation in the porcine small intestine was investigated. Salmonella Typhimurium (10(8) and 10(10) cfu) and cholera toxin (CT; 20 microg) were instilled for 8 and 11 h in ligated loops in the porcine jejunum and ileum. Fluid accumulation and concentrations of Na+, K+, Cl-, 5-HT and PGE2 in the fluid accumulated in the loops were measured. The fluid accumulation was also measured when Salmonella Typhimurium (10(10) cfu) and CT (20 microg) were instilled for 8 h in ligated loops in jejunum and ileum in pigs given subcutaneous injections of saline or the 5-HT3 receptor antagonist ondansetron (200 microg/kg). Salmonella Typhimurium (10(10) cfu) and CT both induced fluid accumulation in jejunum and ileum after 8 and 11 h. Both treatments also induced an increase in luminal release of 5-HT and PGE2. The accumulated fluid was iso-osmotic and hyperosmotic in CT- and Salmonella Typhimurium-treated loops, respectively. Ondansetron reduced the Typhimurium-induced fluid accumulation in both jejunum and ileum by c. 40%, while it failed to reduce the response to CT. These results demonstrate that 5-HT and PGE2 are released and 5-HT3 receptors activated in the secretory pathway of Typhimurium in the porcine small intestine.
Grøndahl
M L
ML
Department of Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Jensen
G M
GM
Nielsen
C G
CG
Skadhauge
E
E
Olsen
J E
JE
Hansen
M B
MB
D000704:Analysis of Variance; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D003967:Diarrhea / Q000503:physiopathology*; D015232:Dinoprostone / Q000032:analysis / Q000378:metabolism; D004195:Disease Models, Animal; D004573:Electrolytes / Q000032:analysis; D007082:Ileum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D007583:Jejunum / Q000737:chemistry / Q000378:metabolism / Q000382:microbiology; D017294:Ondansetron / Q000494:pharmacology; D009994:Osmolar Concentration; D012481:Salmonella Infections, Animal / Q000503:physiopathology*; D012486:Salmonella typhimurium / Q000502:physiology*; D012701:Serotonin / Q000032:analysis / Q000378:metabolism; D012702:Serotonin Antagonists / Q000494:pharmacology; D013552:Swine; D013553:Swine Diseases / Q000503:physiopathology*; D013997:Time Factors
D004573:Electrolytes; D012702:Serotonin Antagonists; D012701:Serotonin; D017294:Ondansetron; D002772:Cholera Toxin; D015232:Dinoprostone