Establishment and characterisation of a monoclonal antibody to inhibit adhesion of Helicobacter pylori to gastric epithelial cells.
Monoclonal antibodies (MAbs) that inhibit adhesion of Helicobacter pylori to human gastric cancer (MKN45) cells were established to clarify the mechanism of adhesion of H. pylori. Of 53 hybridoma clones screened by the primary inhibition assay for adhesion, MAb A20 of IgM class was selected on the basis of both its reactivity to whole cells of H. pylori by ELISA and its inhibitory effect on adhesion of H. pylori. The adhesion of H. pylori strain TK1029 to MKN45 cells was inhibited by MAb A20, depending on the concentration of the MAb. The MAb recognised the surface antigen, lipopolysaccharide (LPS) of H. pylori, suggesting that LPS is associated with adhesion of H. pylori to human gastric epithelial cells.
Osaki
T
T
Division of Flow Cytometry, Kyorin University School of Medicine, Shinkawa, Mitaka, Tokyo, Japan.
Yamaguchi
H
H
Taguchi
H
H
Fukuda
M
M
Kawakami
H
H
Hirano
H
H
Watanabe
S
S
Takagi
A
A
Kamiya
S
S
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D001422:Bacterial Adhesion; D002460:Cell Line; D004847:Epithelial Cells / Q000382:microbiology; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology*; D016480:Helicobacter pylori / Q000276:immunology* / Q000472:pathogenicity* / Q000648:ultrastructure; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D016253:Microscopy, Immunoelectron
D000907:Antibodies, Bacterial; D000911:Antibodies, Monoclonal; D008070:Lipopolysaccharides