Adenovirus-transferred HNF-3 gamma conserves some liver functions in primary cultured hepatocytes of adult rats.
Hepatocyte nuclear factor-3 (HNF-3) isoforms are key factors for regulation of gene expression and differentiation in hepatocytes. HNF-3gamma is abundantly expressed in the mature liver, but down-regulated in primary cultured hepatocytes, in which some other hepatic gene expressions are also decreased. In this study, the primary hepatocytes were infected with the recombinant adenovirus carrying HNF-3gamma gene (AxCAHNF3gamma), and this led to marked induction of the HNF-3gamma gene. As a result, the expressions of albumin, catalase, and ornithine transcarbamylase (OTC) genes were also recovered to significant levels in the AxCAHNF3gamma-infected hepatocytes. Moreover, hepatocyte proliferation stimulated by epidermal growth factor (EGF) and insulin was also inhibited by AxCAHNF3gamma infection. Our results demonstrate that the enforced expression of HNF-3gamma gene can lead to conservation of some original liver functions in the primary cultured hepatocytes accompanied by morphological differentiation and growth inhibition.
Nakamura
T
T
Faculty of Medicine, Tottori University, Yonago, Japan.
Mura
T
T
Saito
K
K
Ohsawa
T
T
Akiyoshi
H
H
Sato
K
K
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D015536:Down-Regulation / Q000235:genetics; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D051543:Hepatocyte Nuclear Factor 3-gamma; D008099:Liver / Q000166:cytology* / Q000378:metabolism / Q000502:physiology*; D008297:Male; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D011995:Recombination, Genetic; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics* / Q000502:physiology
D004268:DNA-Binding Proteins; C496507:Foxa3 protein, rat; D009687:Nuclear Proteins; D014157:Transcription Factors; D051543:Hepatocyte Nuclear Factor 3-gamma