Test characteristics of acridine orange, Gram, and May-Grünwald-Giemsa stains for enumeration of intracellular organisms in bronchoalveolar lavage fluid.
For enumeration of intracellular organisms (ICO) in bronchoalveolar lavage fluid samples, the May-Grünwald-Giemsa (MGG) stain displayed higher interobserver agreement than the acridine orange and Gram stains. The MGG stain offered a reliable enumeration of ICO when 200 cells were counted by one observer.
De Brauwer
E
E
Department of Medical Microbiology, University Hospital Maastricht, The Netherlands.
Jacobs
J
J
Nieman
F
F
Bruggeman
C
C
Drent
M
M
D000165:Acridine Orange; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000382:microbiology*; D015169:Colony Count, Microbial; D004801:Eosine Yellowish-(YS); D005840:Gentian Violet; D006801:Humans; D008751:Methylene Blue; D015588:Observer Variation; D010619:Phenazines; D011014:Pneumonia / Q000175:diagnosis / Q000382:microbiology; D018410:Pneumonia, Bacterial / Q000175:diagnosis / Q000382:microbiology; D015203:Reproducibility of Results; D013194:Staining and Labeling / Q000379:methods*; D012122:Ventilators, Mechanical
C035863:Gram's stain; C043260:May-Grunwald Giemsa; D010619:Phenazines; D000165:Acridine Orange; D005840:Gentian Violet; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)