title: A method for the purification of cAMP-dependent protein kinase using immunoaffinity chromatography.
abstract: A rapid and efficient method for purifying cAMP-dependent protein kinase (PKA) holoenzyme based on immunoaffinity chromatography was developed. The affinity column was prepared by coupling a polyclonal antibody raised against the PKA regulatory subunit to NHS-activated Sepharose. The holoenzyme purified by this procedure from the bivalve mollusk Mytilus galloprovincialis was shown to be fully active as judged by (1) its cAMP-binding activity, (2) its cAMP-dependent protein kinase activity, and (3) its autophosphorylation ability. Moreover, together with both regulatory and catalytic subunits, which constitute the PKA holoenzyme, a protein with a molecular mass of approximately 200 kDa was copurified, and results from gel-filtration chromatography showed that it was associated with a fraction of PKA. Therefore, this immunoaffinity purification technique could also be useful to isolate such proteins as interact with PKA in vivo.
authors.affiliation: Faculty of Veterinary Science, University of Santiago de Compostela, Lugo, 27002, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Cao
authors.forename: M
authors.initials: M
authors.lastname: Fernández
authors.forename: J A
authors.initials: JA
authors.lastname: Villamarín
mesh_terms: D000818:Animals; D049872:Bivalvia / Q000201:enzymology; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D000242:Cyclic AMP / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D007163:Immunosorbent Techniques; D008970:Molecular Weight; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational
chemical_list: D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases