On-line microdialysis coupled with microbore liquid chromatography for the determination of unbound chloramphenicol and its glucuronide in rat blood.
On-line microdialysis coupled with microbore liquid chromatography was used to investigate the pharmacokinetics of chloramphenicol and its glucuronide in rat blood. A microdialysis probe was inserted into a jugular vein of male Sprague-Dawley rats. Chloramphenicol succinate (20 mg/kg, intravenously) was then administered via a femoral vein. Dialysates were automatically injected onto a LC system, via an on-line injector. Samples were eluted with a mobile phase containing acetonitrile-10 mM monochloroacetic acid (30:70, v/v, pH 3.0). The UV detector wavelength was set at 278 nm. The limit of quantitation for chloramphenicol was 10 ng/ml. The in vitro recoveries of chloramphenicol and chloramphenicol glucuronide at 500 ng/ml were 32.2+/-0.3% and 11.4+/-0.7%, respectively (n = 6). Intra- and inter-assay accuracy and precision of the analyses were < or =10% in the range of 0.01 to 5.0 microg/ml.
Tsai
T H
TH
Department of Pharmacology, National Research Institute of Chinese Medicine, National Yang-Ming University, Taipei, Taiwan. thtsai@cma23.nricm.edu.tw
Cheng
F C
FC
Hung
L C
LC
Chen
C F
CF
D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood*; D002701:Chloramphenicol / Q000031:analogs & derivatives* / Q000097:blood*; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D017551:Microdialysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet
D000900:Anti-Bacterial Agents; C027810:chloramphenicol glucuronide; D002701:Chloramphenicol