Targeted inactivation of Dp71, the major non-muscle product of the DMD gene: differential activity of the Dp71 promoter during development.
The dystrophin gene, which is defective in Duchenne muscular dystrophy (DMD), also encodes a number of smaller products controlled by internal promoters. Dp71, which consists of the two C-terminal domains of dystrophin, is the most abundant product of the gene in non-muscle tissues and is the major product in adult brain. To study the possible function of Dp71 and its expression during development, we specifically inactivated the expression of Dp71 by replacing its first and unique exon and a part of the concomitant intron with a beta-galactosidase reporter gene. X-Gal staining of Dp71-null mouse embryos and tissues revealed a very stage- and cell type-specific activity of the Dp71 promoter during development and during differentiation of various tissues, including the nervous system, eyes, limb buds, lungs, blood vessels, vibrissae and hair follicles. High activity of the Dp71 promoter often seemed to be associated with morphogenic events and terminal differentiation. In some tissues the activity greatly increased towards birth.
Sarig
R
R
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel.
Mezger-Lallemand
V
V
Gitelman
I
I
Davis
C
C
Fuchs
O
O
Yaffe
D
D
Nudel
U
U
D000818:Animals; D000831:Animals, Newborn; D016189:Dystrophin / Q000031:analogs & derivatives* / Q000235:genetics; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D017930:Genes, Reporter; D006801:Humans; D007438:Introns; D051379:Mice; D018345:Mice, Knockout; D009137:Muscular Dystrophy, Animal / Q000196:embryology / Q000235:genetics*; D011401:Promoter Regions, Genetic; D001616:beta-Galactosidase / Q000235:genetics
D016189:Dystrophin; C085339:apo-dystrophin 1; D001616:beta-Galactosidase