Effect of postischaemic hypothermia on the mitochondrial damage induced by ischaemia and reperfusion in the gerbil.
In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37 degreesC or 30 degreesC. After normothermic (37 degreesC) ischaemia/reperfusion, significant decreases in mitochondrial state 3 (+ADP) oxygen consumption (-42.2%), complex II-III activity in synaptosomes (-31.7%) and complex IV were measured, in both free mitochondria and synaptosomes (-30.3% and -27. 8% respectively). However, following hypothermic (30 degreesC) reperfusion, both respiration rates and all enzyme activities remained at levels not significantly different from those in the sham operated controls.
Canevari
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Department of Neurochemistry, Institute of Neurology, Queen Square, London WC1N 3BG, UK.l.canevari@ion.ucl.ac.uk
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D000704:Analysis of Variance; D000818:Animals; D002545:Brain Ischemia / Q000473:pathology / Q000628:therapy*; D005849:Gerbillinae; D007036:Hypothermia, Induced; D008928:Mitochondria / Q000473:pathology*; D010101:Oxygen Consumption / Q000502:physiology; D015427:Reperfusion Injury / Q000473:pathology*; D013574:Synaptosomes / Q000473:pathology