The phosphatase Cdc14 triggers mitotic exit by reversal of Cdk-dependent phosphorylation.
Exit from mitosis requires the inactivation of mitotic cyclin-dependent kinases (CDKs) by an unknown mechanism. We show that the Cdc14 phosphatase triggers mitotic exit by three parallel mechanisms, each of which inhibits Cdk activity. Cdc14 dephosphorylates Sic1, a Cdk inhibitor, and Swi5, a transcription factor for SIC1, and induces degradation of mitotic cyclins, likely by dephosphorylating the activator of mitotic cyclin degradation, Cdh1/Hct1. Feedback between these pathways may lead to precipitous collapse of mitotic CDK activity and help coordinate exit from mitosis.
Visintin
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R
Whitehead Institute for Biomedical Research, Cambridge Center, Massachusetts 02142, USA.
Craig
K
K
Hwang
E S
ES
Prinz
S
S
Tyers
M
M
Amon
A
A
D064173:Anaphase-Promoting Complex-Cyclosome; D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D019926:Cyclin B; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D018844:Cyclin-Dependent Kinases / Q000378:metabolism*; D016213:Cyclins / Q000378:metabolism; D004261:DNA Replication / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000378:metabolism / Q000502:physiology; D019204:GTP-Binding Proteins; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D008025:Ligases / Q000378:metabolism / Q000502:physiology; D008938:Mitosis / Q000502:physiology*; D009154:Mutation; D010749:Phosphoprotein Phosphatases / Q000235:genetics / Q000502:physiology; D010766:Phosphorylation; D011494:Protein Kinases / Q000502:physiology; D017346:Protein Serine-Threonine Kinases; D017027:Protein Tyrosine Phosphatases; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins; D013379:Substrate Specificity; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D043743:Ubiquitin-Protein Ligase Complexes; D044767:Ubiquitin-Protein Ligases
C074860:CDC14 protein, S cerevisiae; C082279:CDC15 protein; C577583:CDH1 protein, S cerevisiae; C513239:CEF1 protein, S cerevisiae; C093749:CLB2 protein, S cerevisiae; D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; D019926:Cyclin B; D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C086926:SIC1 protein, S cerevisiae; C080218:SWI5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome; D044767:Ubiquitin-Protein Ligases; D011494:Protein Kinases; C085768:DBF2 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; D010749:Phosphoprotein Phosphatases; D017027:Protein Tyrosine Phosphatases; D019204:GTP-Binding Proteins; D008025:Ligases