title: Temporal changes in disaccharide composition of dermatan sulfate in the skin after epicutaneous application of hapten.
abstract: Glycosaminoglycans change their amount, structure and distribution with age, differentiation and pathologic conditions. In order to investigate temporal changes in dermatan sulfate in the skin during inflammation and subsequent healing, 2,4-dinitrofluorobenzene (DNFB) was applied once to the dorsal skin of female BALB/cA mice, and the disaccharide composition of dermatan sulfate was examined. Application of DNFB induced a transient increase in the thickness of the dermis, which reached a maximum on day 15 and decreased to the control level on day 35. The total amount of unsaturated disaccharides from dermatan sulfate per cm2 in the DNFB-treated skin showed a temporal pattern similar to that of the thickness of the dermis. Mol% of 4,5-unsaturated 2-sulfo-hexuronic acid-4-sulfated N-acetylgalactosamine (deltaDi-diSB), the second most abundant unsaturated disaccharide from dermatan sulfate, decreased rapidly on day 3 in the DNFB-treated skin, remained less than the control on days 7 and 15, and returned to the control level on day 35. These results suggest that a single application of DNFB induces temporal changes not only in the amount but also in the structure of dermatan sulfate.
authors.affiliation: Nippi Research Institute of Biomatrix, Nippi Inc., Tokyo, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Kuwaba
authors.forename: Y
authors.initials: Y
authors.lastname: Nomura
authors.forename: S
authors.initials: S
authors.lastname: Irie
authors.forename: Y
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002642:Chick Embryo; D003871:Dermatan Sulfate / Q000737:chemistry*; D004139:Dinitrofluorobenzene / Q000494:pharmacology; D004187:Disaccharides / Q000737:chemistry*; D004586:Electrophoresis; D005260:Female; D006025:Glycosaminoglycans / Q000737:chemistry; D006241:Haptens / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors
chemical_list: D004187:Disaccharides; D006025:Glycosaminoglycans; D006241:Haptens; D003871:Dermatan Sulfate; D004139:Dinitrofluorobenzene