Gestational and smoking effects on peptidase activity in the placenta.
Angiotensin converting enzyme (ACE) and leucine aminopeptidase (LAP) regulate fetally and maternally generated peptides in the placenta. In this study, ACE-like activity was found to be decreased and LAP-like activity increased with increasing days of gestation in rat placental tissues forming the fetal:maternal interface. Membrane-associated ACE-like and LAP-like activities in the placenta of smokers were also found to be significantly higher than their respective activities in placenta of nonsmokers. Our collective findings suggest that gestational and environmentally-induced changes in placental peptidase activities may account for variable peptide hormone and/or therapeutic peptide metabolism in the placenta.
Kenagy
J
J
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence 66047, USA.
Liu
F
F
Soares
M J
MJ
Audus
K L
KL
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000378:metabolism / Q000502:physiology; D008431:Maternal-Fetal Exchange / Q000502:physiology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism / Q000502:physiology; D010920:Placenta / Q000201:enzymology* / Q000502:physiology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D012907:Smoking / Q000009:adverse effects*; D014327:Trophoblasts; D014407:Tumor Cells, Cultured
D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase; D007703:Peptidyl-Dipeptidase A