title: Conjugation of 5-azido-3-oxapentyl glycosides with thiolated proteins through the use of thiophilic derivatives.
abstract: 5-Azido-3-oxa-pentyl beta-D-galactopyranoside was prepared from diethylene glycol monochlorohydrin and used as a model of oligosaccharide hapten. After deprotection, a series of amides bearing thiophilic groups had been obtained through the terminal amino function and essayed in coupling reactions with thiolated BSA. Also several Lewis human blood group oligosaccharides had been conjugated with thiolated BSA demonstrating the usefulness of the methodology.
authors.affiliation: Laboratory of Synthetic Antigens, Facultad de Química, Universidad de la Habana, Cuba.
authors.forename: V
authors.initials: V
authors.lastname: Fernández-Santana
authors.forename: R
authors.initials: R
authors.lastname: González-Lio
authors.forename: J
authors.initials: J
authors.lastname: Sarracent-Pérez
authors.forename: V
authors.initials: V
authors.lastname: Verez-Bencomo
mesh_terms: D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006027:Glycosides / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D006027:Glycosides; D011506:Proteins; D013438:Sulfhydryl Compounds