Parasite antigen-specific interleukin-10 and antibody reponses predict accelerated parasite clearance in Plasmodium falciparum malaria.
Using strict inclusion criteria, we conducted a hospital-based, case-control study in which 100 Gabonese children with severe Plasmodium falciparum malaria were matched for age, gender and provenance with 100 children presenting with mild malaria. Parasite antigen-specific cellular and humoral immunological responses were measured and compared with post-treatment parasite clearance times in each group. Significantly faster parasite clearance times were associated with in vitro production of IL-10 by acute-phase peripheral blood mononuclear cells (PBMC) in response to both liver and asexual stage parasite antigens, but not with proliferative, IFN-gamma, or TNF responses to the same antigens. In addition, in those children with mild malaria, higher levels of acute-phase antibody responses to liver stage antigen-1 (LSA-1) were associated with faster parasite clearance times, and were correlated with the presence of IL-10 responses to the same antigen. No such associations were found for IL-10 or antibody responses to a range of asexual blood stage antigens. Those with severe malaria had significantly lower levels of anti-LSA-1 antibodies compared to their counterparts with mild malaria. In conclusion, the results of this study suggest that parasite antigen-specific IL-10-mediated antibody responses may play a role in the control of asexual stage parasite multiplication in P. falciparum malaria.
Luty
A J
AJ
University of Tübingen, Institute for Tropical Medicine, Department of Parasitology, Wilhelmstrasse 27, 72074 Tübingen, Germany. Tel: (+49) 7071 2980228, Fax: (+49) 7071 295189, e-mail: adrian. luty@uni-tuebingen.de
Lell
B
B
Schmidt-Ott
R
R
Lehman
L G
LG
Luckner
D
D
Greve
B
B
Matousek
P
P
Herbich
K
K
Schmid
D
D
Ulbert
S
S
Migot-Nabias
F
F
Dubois
B
B
Deloron
P
P
Kremsner
P G
PG
D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D005260:Female; D005681:Gabon; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D016778:Malaria, Falciparum / Q000276:immunology* / Q000469:parasitology*; D008297:Male; D008969:Molecular Sequence Data; D018512:Parasitemia / Q000276:immunology / Q000469:parasitology; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D010963:Plasmodium falciparum / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D010446:Peptide Fragments; D015800:Protozoan Proteins; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma