HLA-E polymorphism in patients with recurrent spontaneous abortion.
The aim of this study was to compare the frequencies of five HLA-E alleles in 82 women with recurrent spontaneous abortions with that of 150 random Danish controls. PCR sequence-specific oligonucleotide typing was carried out to detect polymorphism in exons 2 and 3 of the HLA-E gene. In informative samples sequencing of these two exons was also undertaken to confirm the presence of the HLA-E*01031 allele. HLA-E*0101, HLA-E*01032 and HLA-E*01031 were detected with frequencies of 56.7%, 33.6% and 9.6% in controls and 58,5%, 32.9% and 8.5% in patients with recurrent abortion, respectively. No HLA-E*0102 and E*0104 alleles could be detected. Since the HLA-E allele distribution was similar in women with recurrent spontaneous abortion and controls, it is suggested that maternal HLA-E polymorphism per se does not play any role in the pathogenesis of this disorder of pregnancy.
Steffensen
R
R
Regional Centre for Blood Transfusion and Clinical Immunology, Aalborg Hospital, Denmark. rudi@inet.uni2.dk
Christiansen
O B
OB
Bennett
E P
EP
Jersild
C
C
D000026:Abortion, Habitual / Q000235:genetics* / Q000276:immunology; D000022:Abortion, Spontaneous / Q000235:genetics* / Q000276:immunology; D005260:Female; D006680:HLA Antigens / Q000235:genetics*; D015395:Histocompatibility Antigens Class I / Q000235:genetics*; D006801:Humans; D011110:Polymorphism, Genetic; D011247:Pregnancy; D000097625:HLA-E Antigens
D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D000097625:HLA-E Antigens