Gestational hypertension plasma and human umbilical vein endothelial cells: an in vitro study.
BACKGROUND
The action of plasma from women affected by gestational hypertension (GH) on nitric oxide synthase (NOS) activity in cultured human umbilical vein endothelial cells (HUVECs) was evaluated in the present study, together with the effect on cytosolic calcium, on Na+, K+-ATPase activity and on membrane fluidity.


METHODS
At 80% confluence, cultured HUVECs were incubated for 3 h at 37 degreesC with fresh culture medium (control samples) or with 20% (v/v) plasma (from five healthy non-pregnant women, five healthy pregnant women and five pregnant women affected by GH).


RESULTS
After incubation with GH plasma, we observed a significant reduction in NOS activity, intracellular calcium concentrations and Na+, K+-ATPase activity.


CONCLUSIONS
The present work gives further support to the hypothesis that a circulating factor in gestational hypertension, possibly produced by the fetoplacental unit, causes dysfunction of the vascular endothelial cells and NO reduction, resulting in a loss of vascular refractoriness to vasoactive agents.
Cester
N
N
Università di Ancona, Italy.
Salvolini
E
E
Rabini
R A
RA
Tranquilli
A L
AL
Zolese
G
G
Mazzanti
L
L
D000328:Adult; D002118:Calcium / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood*; D008560:Membrane Fluidity / Q000187:drug effects; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III; D010949:Plasma / Q000502:physiology*; D011247:Pregnancy; D011248:Pregnancy Complications / Q000097:blood*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
C496314:NOS3 protein, human; D019001:Nitric Oxide Synthase; D052250:Nitric Oxide Synthase Type III; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium