Cleavage product from the NO donor NOC-5 and inner ear hair cell damage.
Nitric oxide (NO) is a neurotoxic gas which causes neuronal cell death. NO ototoxicity has also been reported, 1-Hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene (NOC-5), NO donor, was used for studying NO. In this in vitro study, we examined whether NOC-5 and the cleavage products arising from it after production of No have ototoxic effects on the cochlea. The temporal bones of 55 guinea pigs were dissected and incubated in Dulbecco Modified Eagle's Medium (DMEM) with low glucose and 10% foetal bovine serum (FBS) perfused with various concentrations (100, 50, 10, 1 mM, 100 microM) of NOC-5 (pH 7.4) or the same concentrations of N-isopropyl 1-3 propanildiamine (R-NH2). Hair cell death was assessed using trypan blue staining. With NOC-5, percentages of outer hair cell death were 99.2, 98.0, 45.2, 40.5, and 20%, respectively, and percentages of inner hair cell death were 69.3, 70.2, 22.5. 11.1 and 0%, respectively. With R-NH2, percentages of outer hair cell death were 90.3, 81.1, 29.8, 21.0 and 19.3%, respectively, and percentages of inner hair cell death were 61.4, 48.1, 10.2, 5.4 and 0%, respectively. Ultramicroscopic examination showed that hair cell death was necrotic. Although NOC-5 is useful for study of NO, its cleavage products are also ototoxic.
Takayama
M
M
Department of Otolaryngology, Osaka City University Medical School, Japan.
Yamane
H
H
Konishi
K
K
Iguchi
H
H
Nakagawa
T
T
Shibata
S
S
Nishimura
K
K
Sunami
K
K
Nakai
Y
Y
D000818:Animals; D002470:Cell Survival; D003051:Cochlea / Q000187:drug effects / Q000648:ultrastructure; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects* / Q000648:ultrastructure; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000648:ultrastructure; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009569:Nitric Oxide / Q000633:toxicity*; D020030:Nitric Oxide Donors / Q000633:toxicity*; D011437:Propylamines / Q000633:toxicity; D014226:Triazenes / Q000633:toxicity*
C114535:1-hydroxy-2-oxo-3-(3-aminopropyl)-3-isopropyl-1-triazene; D020030:Nitric Oxide Donors; D011437:Propylamines; D014226:Triazenes; D009569:Nitric Oxide