Immunocytochemical demonstration of reduced glutathione in neurons of rat forebrain.
Histochemical studies show reduced glutathione (GSH) in neuroglia, whereas immunocytochemistry of glutaraldehyde-fixed tissue reveals GSH also in neurons. Using an antibody suitable for formaldehyde-fixed tissue, we find GSH staining in the cytoplasm of neurons throughout the brain. Staining was prominent in large pyramidal neurons of cerebral cortex, in basal ganglia, and in reticular and ventrobasal thalamic nuclei.
Maybodi
L
L
Department of Cell Biology and Anatomy, University of North Carolina, CB 7090, Chapel Hill, NC 27599, USA.
Pow
D V
DV
Kharazia
V N
VN
Weinberg
R J
RJ
D000818:Animals; D003593:Cytoplasm / Q000737:chemistry; D005978:Glutathione / Q000032:analysis*; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000737:chemistry* / Q000648:ultrastructure; D016548:Prosencephalon / Q000737:chemistry* / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley
D005978:Glutathione