Paclitaxel stimulates melanogenesis in B16-F1 melanoma cells.
Upon incubation with paclitaxel, B16-F1 melanoma cells showed a flattening and darkening of their cytoplasm. By electron microscopy analysis, an increase in the number of melanosomes associated with an increase of their melanin content was observed. The cells also showed prominent morphological changes such as flattening, loss of prolongation and increase in size. Tyrosinase activity was increased 2.2 fold after 16 hours of incubation with 10 microM paclitaxel. The tyrosinase gene transcription was evaluated by semi-quantitative RT-PCR. The mRNA level was not significantly increased indicating that the effect of paclitaxel on melanogenesis was probably due to post-transcriptional events.
Bicamumpaka
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Department of Biochemistry, Faculty of Medicine, Laval University, Ste Foy, Québec, Canada.
Pagé
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D000818:Animals; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007700:Kinetics; D008546:Melanoma, Experimental / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure*; D020460:Melanosomes / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure*; D051379:Mice; D014442:Monophenol Monooxygenase / Q000235:genetics* / Q000378:metabolism; D017239:Paclitaxel / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000235:genetics; D014407:Tumor Cells, Cultured
D012333:RNA, Messenger; D014442:Monophenol Monooxygenase; D017239:Paclitaxel