title: Role of CDMP-1 in skeletal morphogenesis: promotion of mesenchymal cell recruitment and chondrocyte differentiation.
abstract: Cartilage provides the template for endochondral ossification and is crucial for determining the length and width of the skeleton. Transgenic mice with targeted expression of recombinant cartilage-derived morphogenetic protein-1 (CDMP-1), a member of the bone morphogenetic protein family, were created to investigate the role of CDMP-1 in skeletal formation. The mice exhibited chondrodysplasia with expanded cartilage, which consists of the enlarged hypertrophic zone and the reduced proliferating chondrocyte zone. Histologically, CDMP-1 increased the number of chondroprogenitor cells and accelerated chondrocyte differentiation to hypertrophy. Expression of CDMP-1 in the notochord inhibited vertebral body formation by blocking migration of sclerotome cells to the notochord. These results indicate that CDMP-1 antagonizes the ventralization signals from the notochord. Our study suggests a molecular mechanism by which CDMP-1 regulates the formation, growth, and differentiation of the skeletal elements.
authors.affiliation: Craniofacial Developmental Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: N
authors.initials: N
authors.lastname: Tsumaki
authors.forename: K
authors.initials: K
authors.lastname: Tanaka
authors.forename: E
authors.initials: E
authors.lastname: Arikawa-Hirasawa
authors.forename: T
authors.initials: T
authors.lastname: Nakase
authors.forename: T
authors.initials: T
authors.lastname: Kimura
authors.forename: J T
authors.initials: JT
authors.lastname: Thomas
authors.forename: T
authors.initials: T
authors.lastname: Ochi
authors.forename: F P
authors.initials: FP
authors.lastname: Luyten
authors.forename: Y
authors.initials: Y
authors.lastname: Yamada
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins; D001842:Bone and Bones / Q000196:embryology*; D002356:Cartilage / Q000196:embryology / Q000378:metabolism; D002454:Cell Differentiation; D019902:Chondrocytes / Q000166:cytology*; D015870:Gene Expression; D055428:Growth Differentiation Factor 5; D006133:Growth Substances / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D051379:Mice; D008822:Mice, Transgenic; D009024:Morphogenesis; D013234:Stem Cells
chemical_list: D019485:Bone Morphogenetic Proteins; D055428:Growth Differentiation Factor 5; D006133:Growth Substances