Isotype controls in the analysis of lymphocytes and CD34+ stem and progenitor cells by flow cytometry--time to let go!
Keeney
M
M
Department of Hematology, London Health Sciences Centre, Ontario, Canada. mike.keeney@lhsc.on.ca
Gratama
J W
JW
Chin-Yee
I H
IH
Sutherland
D R
DR
D000911:Antibodies, Monoclonal / Q000032:analysis; D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis* / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D005434:Flow Cytometry / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000032:analysis; D016130:Immunophenotyping; D008214:Lymphocytes / Q000737:chemistry* / Q000276:immunology; D012107:Research Design; D063268:Sialic Acid Binding Ig-like Lectin 3; D013194:Staining and Labeling / Q000379:methods
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018952:Antigens, CD34; D015214:Antigens, Differentiation, Myelomonocytic; D000954:Antigens, Surface; C571163:CD33 protein, human; D007132:Immunoglobulin Isotypes; D063268:Sialic Acid Binding Ig-like Lectin 3