Flow cytometric analysis for enterotoxin exposed on Clostridium perfringens spores.
Flow cytometric method (FCM) with fluorescent-labeled anti-CPE antibody was applied to develop a rapid, specific, and convenient method to detect enterotoxin (CPE) exposed on the surface of spores of Clostridium perfringens. The results obtained indicate that FCM can specifically detect CPE exposed on C. perfringens spores for a short time. Thus, FCM is found to be a rapid, specific, and convenient assay method for detection of CPE exposed on C. perfringens spores, suggesting that it will be hopefully useful to diagnose food poisoning caused by C. perfringens.
Kusunoki
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Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Japan.
Hu
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D
Piyankarage
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RH
Sugii
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S
Uemura
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T
D000818:Animals; D000908:Antibodies, Fungal / Q000276:immunology; D000918:Antibody Specificity; D003015:Clostridium Infections / Q000175:diagnosis*; D003016:Clostridium perfringens / Q000302:isolation & purification* / Q000502:physiology; D004768:Enterotoxins / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000175:diagnosis*; D011817:Rabbits; D013170:Spores
D000908:Antibodies, Fungal; D004768:Enterotoxins; C035598:enterotoxin, Clostridium