title: Prenatal genotyping of Jk(a) and Jk(b) of the human Kidd blood group system by allele-specific polymerase chain reaction.
abstract: An allele-specific polymerase chain reaction (ASPCR) assay for prenatal genotyping of the Kidd antigen system in order to identify pregnancies at risk for haemolytic disease of the newborn (HDN) was developed. Oligonucleotide primers were designed for ASPCR of JKA and JKB. A validation study was performed using DNA isolated from 54 serotyped whole blood samples and 8 amniocentesis samples. A concordance rate of 100 per cent was observed between serotyping and ASPCR detection of the JKA and JKB alleles. Experiments were conducted to quantify the maternal contamination that could be tolerated in Kidd ASPCR assays. The sensitivity of this assay ranged from 0.2 per cent when detecting the presence of JKB and JKA background, to 2 per cent for detecting the presence of JKA in a JKB background. This sensitive assay is particularly useful for rapid genotyping of fetal amniotic cells to identify pregnancies at risk for HDN due to incompatibilities within the Kidd blood group system.
authors.affiliation: Diagnostic Laboratories of The Blood Center, Milwaukee, WI 53233, USA. MJHessner@bcsew.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Hessner
authors.forename: R A
authors.initials: RA
authors.lastname: Pircon
authors.forename: S T
authors.initials: ST
authors.lastname: Johnson
authors.forename: R A
authors.initials: RA
authors.lastname: Luhm
mesh_terms: D000328:Adult; D000483:Alleles; D000649:Amniocentesis; D004247:DNA / Q000032:analysis; D017931:DNA Primers / Q000737:chemistry; D004899:Erythroblastosis, Fetal / Q000097:blood* / Q000175:diagnosis / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D007667:Kidd Blood-Group System / Q000097:blood* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011250:Pregnancy Complications, Hematologic / Q000097:blood* / Q000175:diagnosis; D011296:Prenatal Diagnosis / Q000379:methods*; D015203:Reproducibility of Results; D012703:Serotyping
chemical_list: D017931:DNA Primers; D007667:Kidd Blood-Group System; D004247:DNA