title: Detection and sequence analysis of hepatitis B virus integration in peripheral blood mononuclear cells.
abstract: A PCR-based technique was used to detect hepatitis B virus (HBV) integration in peripheral blood mononuclear cells from patients with chronic hepatitis B. Integrated HBV DNA sequences, with virus-cell junctions located in the cohesive region between direct repeat 1 (DR1) and DR2, were found in 2 of 10 studied patients.
authors.affiliation: Division of Gastroenterology and Hepatology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA. Laskus@pitt.edu
authors.forename: T
authors.initials: T
authors.lastname: Laskus
authors.forename: M
authors.initials: M
authors.lastname: Radkowski
authors.forename: L F
authors.initials: LF
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Nowicki
authors.forename: J
authors.initials: J
authors.lastname: Rakela
mesh_terms: D001483:Base Sequence; D004279:DNA, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D016662:Virus Integration
chemical_list: D004279:DNA, Viral