title: Lumbar motor neuron size and number is affected by age in male F344 rats.
abstract: Motor neurons in the spinal cord of old rats appear similar in size but less numerous compared with those in mature rats; they also contain a large amount of lipofuscin, the lipid peroxidation by-product whose function is largely unknown. The object of this study was to morphometrically characterize motor neurons found in the L4/L5 lumbar spinal cord of mature (6-month) and old (22-month) rats. Paraformaldehyde-fixed, lumbar spinal cords from six rats at each age were embedded in paraffin, sectioned at 6 microm and stained with 0.1% toluidine blue. The nucleolar diameter and area from a minimum of 34 motor neurons per spinal cord were measured. Motor neuron number was calculated using Abercrombie's (Abercrombie, 1946) formula after correcting for tissue shrinkage. Motor neuron number was decreased with age while the neuronal area increased with age. Nucleolar diameter also increased in old rats. Frequency distributions of motor neuron area revealed unimodal distributions of motor neurons rats of both ages. We suggest that larger nucleolar diameter reflects more metabolically active neurons in old rats while larger neuron area is a reflection of the presence of lipofuscin in old motor neurons.
authors.affiliation: Department of Cell Biology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA. jane-jacob@ouhsc.edu
authors.forename: J M
authors.initials: JM
authors.lastname: Jacob
mesh_terms: D000375:Aging / Q000502:physiology*; D000818:Animals; D002452:Cell Count; D002466:Cell Nucleolus; D048429:Cell Size; D008159:Lumbar Vertebrae / Q000166:cytology*; D008297:Male; D009046:Motor Neurons / Q000502:physiology*; D051381:Rats; D011916:Rats, Inbred F344; D013116:Spinal Cord / Q000473:pathology; D016707:Tissue Fixation