title: Reactivity of platelets from healthy individuals and from patients with hematologic diseases following incubation with pegylated recombinant human megakaryocyte growth and development factor in vitro.
abstract: Platelets express the receptor for thrombopoietin. It is possible that thrombopoietin modulates platelet reactivity. We examined the effect of pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF) on platelet activation in vitro using flow cytometry. We compared samples from healthy individuals and from patients with various hematologic diseases (AML, myeloma, postchemotherapy). Citrated whole blood was incubated with PEG-rHuMGDF (10 or 100 ng/ml), then a mild stimulus was added (0.1 U thrombin/ml). Blood from healthy individuals showed a significantly higher degree of platelet activation (CD62p expression), microparticle generation, and aggregate formation after incubation with PEG-rHuMGDF+thrombin versus thrombin alone (p < 0.05). However, this difference could not be shown for platelets from patients with thrombocytopenia or other hematologic diseases. The use of PEG-rHuMGDF should be safe and not cause an additional risk of thromboocclusive disease in these patients.
authors.affiliation: Department of Haematology/Oncology, Justus Liebig University, Giessen, Germany.
authors.forename: A C
authors.initials: AC
authors.lastname: Matzdorff
authors.forename: B
authors.initials: B
authors.lastname: Kemkes-Matthes
authors.forename: H
authors.initials: H
authors.lastname: Pralle
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D006402:Hematologic Diseases / Q000097:blood*; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000503:physiopathology; D008297:Male; D008533:Megakaryocytes / Q000187:drug effects; D008875:Middle Aged; D015539:Platelet Activation / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects; D010976:Platelet Count; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology; D013921:Thrombocytopenia / Q000503:physiopathology; D013926:Thrombopoietin / Q000494:pharmacology*
chemical_list: D011994:Recombinant Proteins; C103117:polyethylene glycol-recombinant human megakaryocyte growth and development factor; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D013926:Thrombopoietin; D013917:Thrombin