title: Visualization of protein-RNA interactions in cytoplasmic polyhedrosis virus.
abstract: Unlike the multiple-shelled organization of other Reoviridae members, cytoplasmic polyhedrosis virus (CPV) has a single-shelled capsid. The three-dimensional structures of full and empty CPV by electron cryomicroscopy show identical outer shells but differ inside. The outer surface reveals a T=1 icosahedral shell decorated with spikes at its icosahedral vertices. The internal space of the empty CPV is unoccupied except for 12 mushroom-shaped densities attributed to the transcriptional enzyme complexes. The ordered double-stranded RNA inside the full capsid forms spherical shells spaced 25 A apart. The RNA-protein interactions suggest a mechanism for RNA transcription and release.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Texas-Houston Medical School, Houston, Texas, USA.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.forename: X
authors.initials: X
authors.lastname: Lu
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Jakana
authors.forename: D H
authors.initials: DH
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhou
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology; D020285:Cryoelectron Microscopy; D012330:RNA, Double-Stranded / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D012087:Reoviridae / Q000648:ultrastructure*; D014764:Viral Proteins / Q000378:metabolism*; D014771:Virion / Q000648:ultrastructure
chemical_list: D012330:RNA, Double-Stranded; D012367:RNA, Viral; D014764:Viral Proteins