title: cDNA cloning, structural organization, and expression of the sheep NRAMP1 gene.
abstract: Mouse resistance to several intracellular pathogens including Mycobacteria, Leishmania, and Salmonella is under the control of the Chromosome (Chr) 1 Natural Resistance Associated Macrophage Protein I gene (Nramp1). This gene could have an economic and health importance for domestic animals and humans as well. Therefore, equivalents of the NRAMP1 gene have been cloned by several research groups in various animal species. To study in sheep the influence of the NRAMP1 gene on the susceptibility to intracellular pathogens induced diseases, we have cloned the sheep NRAMP1 cDNA by screening a splenic cDNA library. The genomic organization of the sheep NRAMP1 gene was then determined by sequencing the exon/intron boundaries. The transcription start points (tsp) from the NRAMP1 mRNA have been located with primer extension experiments. RT-PCR reactions have been used to determine the profile of mRNA expression of this gene.
authors.affiliation: Pathologie Infectieuse et Immunologie, INRA de Tours-Nouzilly, 37380 Nouzilly, France.
authors.forename: V
authors.initials: V
authors.lastname: Bussmann
authors.forename: I
authors.initials: I
authors.lastname: Lantier
authors.forename: F
authors.initials: F
authors.lastname: Pitel
authors.forename: S
authors.initials: S
authors.lastname: Patri
authors.forename: F
authors.initials: F
authors.lastname: Nau
authors.forename: P
authors.initials: P
authors.lastname: Gros
authors.forename: J M
authors.initials: JM
authors.lastname: Elsen
authors.forename: F
authors.initials: F
authors.lastname: Lantier
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D027682:Cation Transport Proteins; D002417:Cattle; D002645:Chickens; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D005091:Exons; D015870:Gene Expression; D005796:Genes / Q000235:genetics*; D006801:Humans; D007438:Introns; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012756:Sheep / Q000235:genetics*; D014018:Tissue Distribution; D014158:Transcription, Genetic; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D002352:Carrier Proteins; D027682:Cation Transport Proteins; D018076:DNA, Complementary; D008565:Membrane Proteins; D012333:RNA, Messenger; D000099173:Natural Resistance-associated Macrophage Protein 1