title: cDNA cloning of a third human C2-domain-containing class II phosphoinositide 3-kinase, PI3K-C2gamma, and chromosomal assignment of this gene (PIK3C2G) to 12p12.
abstract: Phosphoinositide (PI) 3-kinases have been shown to have critical roles in signaling pathways that regulate proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. We have previously used reverse-transcription polymerase chain reaction methods to identify novel PI 3-kinase isoforms in normal human breast and in lymph nodes containing metastatic breast cancer. Here we report the cDNA cloning of a Class II PI 3-kinase found in normal breast tissue. This gene (PIK3C2G) encodes the third distinct protein of the human Class II PI 3-kinase family, PI3K-C2gamma. PIK3C2G was mapped to chromosome 12 at 12p12 by fluorescence in situ hybridization.
authors.affiliation: The Breakthrough Toby Robins Breast Cancer Research Centre, Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, United Kingdom.
authors.forename: M
authors.initials: M
authors.lastname: Rozycka
authors.forename: Y J
authors.initials: YJ
authors.lastname: Lu
authors.forename: R A
authors.initials: RA
authors.lastname: Brown
authors.forename: M R
authors.initials: MR
authors.lastname: Lau
authors.forename: J M
authors.initials: JM
authors.lastname: Shipley
authors.forename: M J
authors.initials: MJ
authors.lastname: Fry
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D003001:Cloning, Molecular; D015870:Gene Expression; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008969:Molecular Sequence Data; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics* / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014018:Tissue Distribution
chemical_list: D019869:Phosphatidylinositol 3-Kinases