[Introduction and long-term storage of recombinant luminescent Escherichia coli strain Z905 in laboratory water microecosystems].
We studied preservation of recombinant Escherichia coli strain Z905 (AprLux+) in liquid microecosystems (LME) after the introduction. E. coli cells were shown to remain viable and preserve the ability to express the cloned lux genes for a long time (more than a year) in LME. The majority of the clones have reduced efficiency of the expression due to either changed regulation of the lux operon or decreased number of copies of the plasmid. These mechanisms could be realized either independently or simultaneously depending on LME conditions. We have exposed the major factors affecting the metabolic activity of the E. coli strain Z905 (AprLux+) introduced into model ecosystems and the level of expression of the cloned genes.
Popova
L Iu
LIu
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russia.
Maksimova
E E
EE
Kargatova
T V
TV
Bril'kov
A V
AV
Pechurkin
N S
NS
D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D007753:Laboratories; D008163:Luminescent Measurements; D011995:Recombination, Genetic; D014871:Water Microbiology