A novel member of the NF2/ERM/4.1 superfamily with growth suppressing properties in lung cancer.
A novel putative tumor suppressor gene and member of the NF2/ERM/ 4.1 superfamily was isolated using Differential Display PCR (DDPCR) on primary lung tumors. When reintroduced into nonexpressing non-small cell lung carcinoma cell lines, this gene, named DAL-1 (for Differentially expressed in Adenocarcinoma of the Lung), was shown to suppress growth. In addition, significantly reduced expression (>50%) of DAL-1 was measured in 39 primary non-small cell lung carcinoma tumors as compared with patient-matched normal lung tissue. Immunocytochemical staining with a polyclonal anti-DAL-1 antibody localized the protein to the plasma membrane, particularly at cell-cell contact points, a pattern reminiscent of other members of the protein 4.1 superfamily including ezrin and NF2. The data suggest DAL-1 is a novel membrane-associated protein with potential to play an important role in the origin and progression of lung cancer.
Tran
Y K
YK
University of California-San Diego Cancer Center, University of California-San Diego, La Jolla 92093, USA.
Bögler
O
O
Gorse
K M
KM
Wieland
I
I
Green
M R
MR
Newsham
I F
IF
D000595:Amino Acid Sequence; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D003598:Cytoskeletal Proteins; D015972:Gene Expression Regulation, Neoplastic; D016515:Genes, Neurofibromatosis 2; D016147:Genes, Tumor Suppressor; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D009479:Neuropeptides; D010750:Phosphoproteins / Q000235:genetics; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D025521:Tumor Suppressor Proteins; D000099149:Ezrin
D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D009479:Neuropeptides; D010750:Phosphoproteins; D025521:Tumor Suppressor Proteins; D000099149:Ezrin; C116887:EPB41L3 protein, human; C028841:erythrocyte membrane band 4.1 protein; C120557:erythrocyte membrane protein band 4.1-like 1