CA 125 is dominantly expressed by arrest of G0/G1 phase in uterine cervical adenocarcinoma cell line.
Our purpose is to evaluate at which phase of the cell cycle CA 125 is dominantly expressed in a uterine cervical adenocarcinoma cell line (CAC-1). By a flow cytometric analysis of CA 125 expression, approximately 50% of the cells treated with interferon-gamma (IFN) were positive after 24 h. In cell cycle, the G0/G1 arrest was observed in cells treated with IFN after 24 h. The pattern for the transition of the relative rate of CA 125 expression was similar to that of the population of G0/G1. Furthermore, in the G0/G1 phase, the positive rate of CA 125 expression in cells treated with IFN was 80% of the expression in all cells after 24 h. Additionally, flow cytometry with Ki-67 demonstrated that G1 arrest occurred at 24 h, and then G0 arrest was induced by IFN after 48 h of incubation. The increased expression of CA 125 appeared mainly in the G1 phase rather than in G0.
Hayashi
T
T
Department of Obstetrics and Gynecology, Sapporo Medical University School of Medicine, Sapporo, 060, Japan.
Hayakawa
O
O
Kudo
R
R
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism*; D018394:CA-125 Antigen / Q000096:biosynthesis*; D005260:Female; D016193:G1 Phase; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology; D016192:Resting Phase, Cell Cycle; D014407:Tumor Cells, Cultured; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism*
D018394:CA-125 Antigen; D007371:Interferon-gamma