Rapid induction of primary human CD4+ and CD8+ T cell responses against cancer-associated MUC1 peptide epitopes.
Antigen-specific MHC class II- and class I-restricted helper and cytotoxic T cell responses are important anti-cancer immune responses. MUC1 mucin is a potentially important target for immunotherapy because of its high expression on most human adenocarcinomas. MUC1 peptide-specific type 1 T cell responses were generated in vitro using human peripheral blood lymphocytes (PBL), incubated with liposomes containing synthetic MUC1 lipopeptide antigen. Only two weekly stimulations with the liposomal MUC1 formulation led to the generation of potent anti-MUC1-specific T cell proliferation as well as class I-restricted cytotoxic responses. Thus the use of PBL pulsed with liposome-encapsulated antigen provides an effective approach of rapidly generating effective antigen-presenting cell (APC) function as well as antigen specific T cells in vitro. It may be feasible to use this technology for the rapid and effective generation of APC and/or T cells as cellular vaccines for adenocarcinomas.
Agrawal
B
B
Biomira Inc., Edmonton, Alberta, Canada.
Krantz
M J
MJ
Reddish
M A
MA
Longenecker
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D006801:Humans; D008074:Lipoproteins / Q000276:immunology / Q000494:pharmacology; D008081:Liposomes / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D008969:Molecular Sequence Data; D018396:Mucin-1; D009842:Oligopeptides / Q000276:immunology* / Q000494:pharmacology; D010446:Peptide Fragments
D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D008074:Lipoproteins; D008081:Liposomes; C116055:MUC1 tandem repeat peptide; D018396:Mucin-1; D009842:Oligopeptides; D010446:Peptide Fragments