Occurrence of reducing terminal N-acetylglucosamine 3-sulfate and fucosylated outer chains in acidic N-glycans of porcine zona pellucida glycoproteins.
Structures of acidic N-glycans released from porcine zona pellucida glycoproteins by hydrazinolysis were studied. The results indicated that the acidic glycans are of mono- to tetraantennary complex-type with and without N-acetyllactosamine repeating units. Sulfated residues are not only located at the C-6 position of GlcNAc included in the N-acetyllactosamine repeating units, but also at the C-6 position of GlcNAc in the non-repeated antennae and at the C-3 position of reducing terminal GlcNAc residue. Analysis of the oligosaccharide fragments released by endo-beta-galactosidase digestion and by hydrazine/nitrous acid treatment also revealed that various sulfated and non-sulfated forms of fucosylated structures such as Fucalpha1 --> 2Galbeta1 --> 4(+/-SO3- --> 6)GlcNAc (type 2H), Galbeta1 --> 4(Fucalpha1 --> 3)(+/-SO3- --> 6)GlcNAc(Lex) and Fucalpha1 --> 3 or 4(+/-SO3- --> 6)GlcNAc, are expressed in the repeated outer chain moieties.
Mori
E
E
Department of Immunology, Institute of Medical Science, University of Tokyo, Japan.
Hedrick
J L
JL
Wardrip
N J
NJ
Mori
T
T
Takasaki
S
S
D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange; D003641:Deamination; D004527:Egg Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008562:Membrane Glycoproteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry*; D011956:Receptors, Cell Surface; D013431:Sulfates / Q000737:chemistry; D013552:Swine; D000072216:Zona Pellucida Glycoproteins
D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011134:Polysaccharides; D011956:Receptors, Cell Surface; D013431:Sulfates; D000072216:Zona Pellucida Glycoproteins; D000117:Acetylglucosamine