Targeted mutagenesis by duplication insertion in the radioresistant bacterium Deinococcus radiodurans: radiation sensitivities of catalase (katA) and superoxide dismutase (sodA) mutants.
Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.
Markillie
L M
LM
Pacific Northwest National Laboratory, Molecular Biosciences, Richland, Washington 99352, USA.
Varnum
S M
SM
Hradecky
P
P
Wong
K K
KK
D002374:Catalase / Q000235:genetics*; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D004926:Escherichia coli / Q000235:genetics; D005720:Gamma Rays; D008837:Micrococcus / Q000201:enzymology / Q000235:genetics* / Q000528:radiation effects*; D016254:Mutagenesis, Insertional; D018384:Oxidative Stress; D011836:Radiation Tolerance; D013482:Superoxide Dismutase / Q000235:genetics*
D003037:Cobalt Radioisotopes; D002374:Catalase; D013482:Superoxide Dismutase