title: Effects of characterised Pseudomonas aeruginosa exopolysaccharides on adherence to human tracheal cells.
abstract: This study evaluated, in vitro, the role of different Pseudomonas aeruginosa exopolysaccharides (EPS) in mediating adherence to human respiratory epithelial cells. Two mucoid and non-mucoid isogenic pairs of P aeruginosa strains isolated from patients with cystic fibrosis (CF) and bronchiectasis were used. Adherence was tested with human tracheal epithelial cell lines from CF and normal fetuses. The CF cells bound significantly more bacteria than the normal cells. The strain from the bronchiectasis patient was significantly more adherent than that from the CF patient and this difference was consistently most marked with the non-mucoid variant and with normal epithelial cells. The differing behaviour of mucoid CF and non-mucoid bronchiectasis strains reflected the chemical composition of their EPS: mainly alginate in the former and neutral polysaccharides in the latter. Additive inhibition experiments with chemically characterised EPS indicated that neutral polysaccharides associated with alginate may act as ligands for the adherence of P. aeruginosa to CF epithelial cells.
authors.affiliation: Laboratoire de Microbiologie, Université Paul Sabatier, Toulouse, France.
authors.forename: N
authors.initials: N
authors.lastname: Marty
authors.forename: C
authors.initials: C
authors.lastname: Pasquier
authors.forename: J L
authors.initials: JL
authors.lastname: Dournes
authors.forename: K
authors.initials: K
authors.lastname: Chemin
authors.forename: F
authors.initials: F
authors.lastname: Chavagnat
authors.forename: M
authors.initials: M
authors.lastname: Guinand
authors.forename: G
authors.initials: G
authors.lastname: Chabanon
authors.forename: B
authors.initials: B
authors.lastname: Pipy
authors.forename: H
authors.initials: H
authors.lastname: Montrozier
mesh_terms: D000464:Alginates / Q000032:analysis / Q000737:chemistry; D001422:Bacterial Adhesion; D001987:Bronchiectasis / Q000382:microbiology; D002241:Carbohydrates / Q000032:analysis; D003550:Cystic Fibrosis / Q000382:microbiology; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000502:physiology*; D011550:Pseudomonas aeruginosa / Q000737:chemistry / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology; D013997:Time Factors; D014132:Trachea / Q000382:microbiology*
chemical_list: D000464:Alginates; D002241:Carbohydrates; D011135:Polysaccharides, Bacterial; C064841:exopolysaccharide, Pseudomonas