Characterization of a cloned xenopus laevis serotonin 5-HT1A receptor expressed in the NIH-3T3 cell line.
In a previous work we isolated a Xenopus 5-HT1A receptor gene and now report the characterization of this receptor. The HindIII-XbaI fragment of this gene was cloned into the pcDNA I NEO vector and stably transfected into eukaryotic cells (NIH-3T3). To determine the specific 5-HT1A receptor binding, [3H]8-OH-DPAT was used as radioligand. The selective 5-HT1A receptor agonist bound only a single class of saturable high-affinity binding sites with pharmacological characteristics similar to those of the mammalian 5-HT1A receptor. The effects of X5-HT1A receptor activation on cell growth were also investigated in stably transfected NIH-3T3 cells. The 5-HT1A agonist 8-OH-DPAT was found to increase DNA synthesis and accelerated cell growth.
Cappellini
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Laboratori di Biologia Cellulare e dello Sviluppo, Dipartimento di Fisiologia e Biochimica, Università di Pisa, via Carducci 13, 56010 Ghezzano, Pisa, Italy.
Malatesta
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Costa
B
B
Marracci
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S
Nardi
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I
Martini
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C
D016475:3T3 Cells; D000818:Animals; D002065:Buspirone / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000187:drug effects*; D007650:Ketanserin / Q000494:pharmacology; D051379:Mice; D010869:Pindolol / Q000494:pharmacology; D011985:Receptors, Serotonin / Q000187:drug effects / Q000235:genetics*; D012701:Serotonin / Q000494:pharmacology; D014982:Xenopus laevis
D011985:Receptors, Serotonin; D012701:Serotonin; D007650:Ketanserin; D010869:Pindolol; D002065:Buspirone