title: Kinetic properties and stereospecificity of the monomeric dUTPase from herpes simplex virus type 1.
abstract: Kinetic properties of the monomeric enzyme dUTPase from herpes simplex virus type 1 (HSV) were investigated and compared to those previously determined for homotrimeric dUTPases of bacterial and retroviral origins. The HSV and Escherichia coli dUTPases are equally potent as catalysts towards the native substrate dUTP with a kcat/K(M) of about 10(7) M(-1) s(-1) and a K(M) of 0.3 microM. However, the viral enzymes are less specific than the bacterial enzyme. The HSV and E. coli dUTPases show the same stereospecificity towards the racemic substrate analogue dUTPalphaS (2'-deoxyuridine 5'-(alpha-thio)triphosphate), suggesting that they have identical reaction mechanisms.
authors.affiliation: Department of Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, Sweden.
authors.forename: A C
authors.initials: AC
authors.lastname: Bergman
authors.forename: P O
authors.initials: PO
authors.lastname: Nyman
authors.forename: G
authors.initials: G
authors.lastname: Larsson
mesh_terms: D002384:Catalysis; D003856:Deoxyuracil Nucleotides / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D004860:Infectious Anemia Virus, Equine / Q000201:enzymology; D007700:Kinetics; D008324:Mammary Tumor Virus, Mouse / Q000201:enzymology; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D003856:Deoxyuracil Nucleotides; D004791:Enzyme Inhibitors; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase