Chromosomal organization and nucleotide sequence of the genes coding for the elongation factors G and Tu of the apple proliferation phytoplasma.
Genes coding for elongation factors G (fus) and Tu (tuf) of the non-culturable apple proliferation (AP) phytoplasma were cloned and sequenced. Arrangement of these genes and identification of the ribosomal protein gene rps7 upstream of the fus gene suggest a transcriptional organization similar to that of the streptomycin operon of Escherichia coli and other bacteria. The fus and tuf genes from other tested phytoplasmas were found to be similarly linked as in the AP agent. Thus, it is likely that they show a similar chromosomal arrangement. This organization would be in contrast to that of the phylogenetically distinctly different culturable mollicutes of the genus Mycoplasma in which the tuf and fus genes are separately transcribed.
Berg
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Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut f ur Pflanzenschutz im Obstbau, D-69221, Dossenheim, Germany.
Seemüller
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E
D000595:Amino Acid Sequence; D001419:Bacteria / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular; D017931:DNA Primers; D008040:Genetic Linkage; D008969:Molecular Sequence Data; D020653:Peptide Elongation Factor G; D010444:Peptide Elongation Factor Tu / Q000235:genetics*; D010445:Peptide Elongation Factors / Q000235:genetics*; D016133:Polymerase Chain Reaction; D019685:Rosales / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D008973:Tenericutes / Q000235:genetics*
D017931:DNA Primers; D020653:Peptide Elongation Factor G; D010445:Peptide Elongation Factors; D010444:Peptide Elongation Factor Tu