Crystallization and preliminary X-ray diffraction analysis of group 5 mite allergen from Dermatophagoides pteronyssinus.
Crystals of the 14-kDa group 5 allergen from Dermatophagoides pteronyssinus (Der p 5) have been obtained at low pH and diffract to 3-A resolution using a conventional x-ray source. The crystals belong to tetragonal space group P41212 or P43212, with unit cell parameters a = b = 114 A and c = 234 A. A self-rotation search revealed a 432 point symmetry and thus suggested 96 molecules in one unit cell, hence 12 monomers in each asymmetric unit.
Liaw
S H
SH
Institute of Molecular Medicine, College of Medicine, Taipei, Taiwan. shliaw@ym.edu.tw
Chen
H Z
HZ
Kuo
I C
IC
Chua
K Y
KY
D000818:Animals; D039741:Antigens, Dermatophagoides; D003460:Crystallization; D018360:Crystallography, X-Ray; D006023:Glycoproteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D019476:Insect Proteins / Q000737:chemistry; D008925:Mites / Q000276:immunology*; D011994:Recombinant Proteins / Q000737:chemistry
D039741:Antigens, Dermatophagoides; D006023:Glycoproteins; D019476:Insect Proteins; D011994:Recombinant Proteins