Effects of recombinant human soluble interleukin-4 receptor on interleukin-4/staphylococcal enterotoxin B-stimulated peripheral mononuclear cells from patients with atopic eczema.
In atopic eczema both in local inflammatory reactions and in peripheral blood high interleukin (IL) 4: interferon-gamma (IFN-gamma) production ratios have been demonstrated, indicating predominance of TH2 cell subsets resulting in increased IL-4 production and high serum IgE. The in vitro immunomodulatory effects of recombinant human soluble IL-4 receptor (rsIL-4R) on IL-4-stimulated lymphocyte proliferation, IgE and IFN-gamma production were studied in peripheral blood mononuclear cells from 10 patients with atopic eczema and seven healthy donors. In addition to control cultures (without any stimulus) and cultures with simultaneous application of rsIL-4R and IL-4, time-kinetic experiments were performed. We further investigated the influence of rsIL-4R on IL-4 production in staphylococcal enterotoxin B (SEB) stimulated peripheral blood mononuclear cells. Early addition of rsIL-4R to IL-4-stimulated peripheral blood mononuclear cells resulted in an increase in IFN-gamma production and in suppression of IL-4 induced proliferation and IgE secretion. Unexpectedly, rsIL-4R in combination with SEB exhibited an IL-4 protective effect with a significant increase in detectable IL-4 in the culture supernatants. The present data support the assumption that rsIL-4R might be a promising new immunomodulatory substance in the treatment of atopic eczema.
Sperhake
K
K
Department of Pathology, University Hospital Eppendorf, Martinistr. 52, D-20246 Hamburg, Germany.
Neuber
K
K
Enssle
K
K
Ring
J
J
D000293:Adolescent; D000328:Adult; D018929:Cell Culture Techniques; D002455:Cell Division / Q000187:drug effects; D003876:Dermatitis, Atopic / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D008875:Middle Aged; D019948:Receptors, Interleukin-4 / Q000276:immunology*; D011994:Recombinant Proteins / Q000494:pharmacology; D018089:Superantigens / Q000276:immunology
D004768:Enterotoxins; D019948:Receptors, Interleukin-4; D011994:Recombinant Proteins; D018089:Superantigens; D015847:Interleukin-4; D007073:Immunoglobulin E; C031818:enterotoxin B, staphylococcal; D007371:Interferon-gamma