Immunolocalization of transforming growth factor-beta s and type I and type II receptors in rat articular cartilage.
The activities of TGF-beta s and their receptors (T beta R) in the articular cartilage have not been fully elucidated as yet. Temporal and spatial expression of TGF-beta s and their receptors were examined immunohistochemically in the articular cartilage of growing rats to clarify their role in chondrogenesis. TGF-beta 1, -beta 2 and -beta 3 were strongly expressed from 6 to 50 weeks in the superficial, transitional, and least mature zones. The expression of TGF-beta s in hypertrophic chondrocyte was weak except TGF-beta 1. T beta R-I and T beta R-II were co-expressed with the ligand in the superficial, transitional, and least mature zones throughout the growth phase. This study revealed that TGF-beta s in the articular cartilage plays a major role in the modulation of endochondral ossification ensuring the growth and maintenance of the chondrocyte lineage in articular cartilage.
Fukumura
K
K
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Japan.
Matsunaga
S
S
Yamamoto
T
T
Nagamine
T
T
Ishidou
Y
Y
Sakou
T
T
D030201:Activin Receptors, Type I; D000375:Aging / Q000378:metabolism*; D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000032:analysis / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000032:analysis / Q000235:genetics*
D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; D017346:Protein Serine-Threonine Kinases; D030201:Activin Receptors, Type I; D000077293:Receptor, Transforming Growth Factor-beta Type I; D000077294:Receptor, Transforming Growth Factor-beta Type II; C000625843:Tgfbr1 protein, rat