Cloning of zebrafish cDNA for 3beta-hydroxysteroid dehydrogenase and P450scc.
P450scc and 3beta-HSD cDNA were isolated from a zebrafish lambda gt10 cDNA library using trout SCC and 3beta-HSD cDNA as the probes. The zebrafish SCC cDNA encodes a protein of 509 amino acids, which shares a 78% similarities with the trout SCC and 58% with the human SCC. As for 3beta-HSD, two forms of cDNA were isolated, termed HSD 5 and HSD 17, which may have resulted from alternative splicing. HSD 5 and HSD 17 encode proteins of 374 and 341 amino acids respectively. Both share 77% amino acid similarities with trout 3beta-HSD and 53% similarities with the mouse 3beta-HSD. Zebrafish has been increasingly used as a genetic model system to study organ development and to investigate human diseases. The cloning and the characterization of zebrafish P450scc and 3beta-HSD should facilitate study of steroidogenesis and human disease associated with steroid imbalance.
Lai
W W
WW
Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China.
Hsiao
P H
PH
Guiguen
Y
Y
Chung
B C
BC
D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics*; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics; D017686:Oncorhynchus mykiss / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D015027:Zebrafish / Q000235:genetics*
D018076:DNA, Complementary; D007527:Isoenzymes; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme