title: Comparison of IL-10 levels in chronic venous insufficiency ulcers and autologous donor tissue.
abstract: In previous immunohistochemical studies, chronic venous insufficiency (CVI) ulcers have been shown to display positive staining for interleukin-10 (IL-10), while other wounds (including autologous donor wound tissue) show a reduced staining level. IL-10 inhibits the synthesis of many proinflammatory cytokines, while also inhibiting antigen presentation by antigen-presenting cells. It is possible that abnormally high amounts of IL-10 in chronic wounds may be related to the failure of these wounds to progress to final wound healing. The purpose of this study was to quantify the levels of IL-10 in CVI ulcers and autologous donor tissue using Western blotting. Extracts were prepared from frozen wound tissue samples and equal amounts of protein were concentrated by immune-precipitation for Western blot analysis. Densitometric analysis was performed on nonsaturated chemilumigraphs and normalized to an IL-10 standard run on each gel. The quantity of IL-10 in CVI ulcers was found to be 490% of the quantity in autologous donor tissue. This study provides confirmatory quantitative data which supports previous immunohistochemical findings showing elevated levels of IL-10 in CVI ulcers.
authors.affiliation: University of Massachusetts Medical Center, Division of Plastic Surgery, Worcester 01655, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Lundberg
authors.forename: T P
authors.initials: TP
authors.lastname: Roth
authors.forename: R M
authors.initials: RM
authors.lastname: Dunn
authors.forename: J W
authors.initials: JW
authors.lastname: Doyle
mesh_terms: D015153:Blotting, Western; D002908:Chronic Disease; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000502:physiology; D006801:Humans; D016753:Interleukin-10 / Q000032:analysis*; D011233:Precipitin Tests; D014456:Ulcer / Q000378:metabolism*; D014689:Venous Insufficiency / Q000378:metabolism*; D014945:Wound Healing
chemical_list: D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor