Identification of MEN1 mutations in sporadic enteropancreatic neuroendocrine tumors by analysis of paraffin-embedded tissue.
Gastrinomas and other gastrointestinal neuroendocrine tumors may occur sporadically or as part of the inherited syndrome multiple endocrine neoplasia type 1 (MEN1). Mutations in the recently identified MEN1 gene have been described in sporadic gastrinomas and insulinomas. This study describes techniques used to identify mutations in the MEN1 gene in DNA extracted from paraffin-preserved tissue. Two novel mutations are identified in the MEN1 gene from nine archived paraffin-embedded neuroendocrine tumors, demonstrating that retrospective genetic analysis can be used to identify mutations in the MEN1 gene from preserved tissue. Conditions are provided by which paraffin-embedded tissue can be used as a source of genetic material for sequence information of sufficient quality for mutational studies of the MEN1 gene. It should also be possible to apply this retrospective genetic analysis of paraffin-embedded tissue to other disease models.
Mailman
M D
MD
Department of Pathology, The Ohio State University College of Medicine, Columbus 43210, USA.
Muscarella
P
P
Schirmer
W J
WJ
Ellison
E C
EC
O'Dorisio
T M
TM
Prior
T W
TW
D004273:DNA, Neoplasm / Q000032:analysis; D004379:Duodenal Neoplasms / Q000235:genetics / Q000473:pathology; D015408:Gastrinoma / Q000235:genetics / Q000473:pathology; D005770:Gastrointestinal Neoplasms / Q000235:genetics* / Q000473:pathology; D006579:Heterozygote; D006801:Humans; D007340:Insulinoma / Q000235:genetics / Q000473:pathology; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology; D018761:Multiple Endocrine Neoplasia Type 1 / Q000235:genetics*; D009154:Mutation; D009363:Neoplasm Proteins / Q000235:genetics*; D018358:Neuroendocrine Tumors / Q000235:genetics* / Q000473:pathology; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology; D016612:Paraffin Embedding; D011518:Proto-Oncogene Proteins; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology
D004273:DNA, Neoplasm; C105373:MEN1 protein, human; D009363:Neoplasm Proteins; D011518:Proto-Oncogene Proteins