Cyclin D3 expression, cell proliferation and pathological stage of human primary colorectal cancer.
Cyclin D3 promotes cell cycle progression but its expression and prognostic significance in human colorectal cancer is unknown. This study assayed cyclin D3 expression against cell cycle phase fraction and Duke's stage in 35 fresh human primary colorectal cancers. DNA content, cell cycle phase fraction and cyclin D3 expression were assessed by flow cytometry in disaggregated tumors. Cyclin D3 expression and S-phase fraction were independently related to Duke's stage. In Duke's stage C tumors, a higher proportion of cells expressed cyclin D3 (14.4 vs. 8.8%, mean; p < 0.05 by Mann-Whitney U test) and were in DNA synthesis (S) phase (21.1 vs. 9.7%, mean; p < 0.05 by Mann-Whitney U test). Neoplastic deregulation of cyclin D3 expression may provide a selective growth advantage which is related to stage in human colorectal cancer.
Watson
D S
DS
Department of Surgery, The Medical School, University of Newcastle, Newcastle upon Tyne, UK.
Brotherick
I
I
Shenton
B K
BK
Wilson
R G
RG
Angus
B
B
Varma
J S
JS
Campbell
F C
FC
D000368:Aged; D000369:Aged, 80 and over; D002455:Cell Division; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology*; D056743:Cyclin D3; D016213:Cyclins / Q000032:analysis* / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging
C534696:CCND3 protein, human; D056743:Cyclin D3; D016213:Cyclins