Metabolism of the dimethyl ester of [2,3-(13)C]succinic acid in rat hepatocytes.
Hepatocytes prepared from overnight fasted rats were incubated for 120 min in the presence of the dimethyl ester of [2,3-(13)C]succinic acid (10 mM). The identification and quantification of 13C-enriched metabolites in the incubation medium were performed by a novel computational strategy for the deconvolution of NMR spectra with multiplet structures and constraints. The generation of 13C-labelled metabolites, including succinate, fumarate, malate, lactate, alanine, aspartate and glucose accounted for about half of the initial amount of the ester present in the incubation medium. A fair correlation was observed between the experimental abundance of each 13C-labelled glucose isotopomer and the corresponding values derived from a model for the metabolism of [2,3-(13)C]succinate. Newly formed glucose was more efficiently labelled in the carbon C5 than C2, as well as the carbon C6 than C1, supporting the concept that D-glyceraldehyde-3-phosphate may undergo enzyme-to-enzyme channelling between glyceraldehyde-3-phosphate dehydrogenase and phosphofructoaldolase.
Malaisse
W J
WJ
Laboratory of Experimental Medicine, Brussels Free University (ULB), Belgium.
Ladrière
L
L
Jijakli
H
H
Laatikainen
R
R
Niemitz
M
M
Verbruggen
I
I
Biesernans
M
M
Willem
R
R
D000409:Alanine / Q000378:metabolism; D000818:Animals; D004952:Esters / Q000378:metabolism*; D005260:Female; D005650:Fumarates / Q000378:metabolism; D066298:In Vitro Techniques; D019344:Lactic Acid / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008293:Malates / Q000378:metabolism; D008956:Models, Chemical; D051381:Rats; D017208:Rats, Wistar; D013386:Succinates / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism
D004952:Esters; D005650:Fumarates; D008293:Malates; D013386:Succinates; D019344:Lactic Acid; C030298:malic acid; C032005:fumaric acid; C056451:dimethyl succinate; D019802:Succinic Acid; D000409:Alanine