Compression of the DNA minor groove is responsible for termination of DNA synthesis by HIV-1 reverse transcriptase.
HIV-1 reverse transcriptase (RT) generally terminates plus strand DNA synthesis at the centre of the viral genome. The central termination sequence (CTS) contains two termination sites which are located at the 3' end of AnTm motifs. These motifs generate a global curvature of the DNA helix which correlates with termination of DNA synthesis. Here, we have characterized HIV-1 RT termination sites on different DNA sequences. Again, they are located at the 3' end of A-tracts. Using hydroxyl radicals as a probe of the width of the DNA helix, we have shown that RT termination sites are always located a few base-pairs downstream of a compressed minor groove. Mutations which relieve these compressions also abolish the termination events. The replacement of the adenine tracts by 2,6-diaminopurine tracts has a similar effect. Moreover, no termination site is observed on DNA sequences containing phased GC-tracts which curve the DNA helix but compress the major groove. The compression of the DNA minor groove and not necessarily the curved trajectory of the DNA is, therefore, responsible for termination of DNA synthesis at the CTS by HIV-1 RT. This conclusion is consistent with interpretation of other biochemical data on the processivity of HIV-1 RT, based on the structure of a DNA-enzyme complex.
Lavigne
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M
Unité de Physicochimie des Macromolécules Biologiques (CNRS URA 1773), Institut Pasteur, 75724 Paris Cedex 15, France. mlavigne@pasteur.fr
Buc
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H
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000378:metabolism; D000225:Adenine / Q000031:analogs & derivatives; D001483:Base Sequence; D004247:DNA / Q000096:biosynthesis* / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics*; D015497:HIV-1 / Q000201:enzymology*; D017665:Hydroxyl Radical / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D012194:RNA-Directed DNA Polymerase
D004279:DNA, Viral; D017665:Hydroxyl Radical; D015075:2-Aminopurine; C007300:2,6-diaminopurine; D004247:DNA; D054303:HIV Reverse Transcriptase; D012194:RNA-Directed DNA Polymerase; D000225:Adenine