title: IFN gamma and IL-10 production by hepatic lymph node and peripheral blood lymphocytes in Fasciola hepatica infected sheep.
abstract: The present study was designed to determine whether IFN gamma and IL-10 were secreted by peripheral blood mononuclear cells (PBMC) and hepatic lymph node mononuclear cells (HLNMC) from 17 Fasciola hepatica infected sheep after in vitro stimulation by Fasciola hepatica excretory-secretory products (FhESP) during the first six weeks of two different infections. The IFN gamma concentration in mononuclear cells (MC) culture supernatants was determined by sandwich ELISA. The presence of IL-10 in MC culture supernatants was determined by the capacity of these to inhibit, with or without monoclonal antibodies to IL-10, the NO production of recombinant bovine IFN gamma (rBoIFN gamma) activated monocytes. FhESP-stimulated PBMC and HLNMC produced IFN gamma in culture supernatants from DPI 7 (day post-infestation 7) to DPI 14. Culture supernatants of PBMC stimulated with FhESP first induced an increase in the NO production of rBoIFN gamma activated monocytes. After 2 or 3 weeks of infection, MC culture supernatant reduced the NO production of rBoIFN gamma activated monocytes. From DPI 35, anti-IL-10 antibodies addition restored or enhanced NO production. During sheep fasciolosis, IFN gamma production increased only until the first two weeks post infection. IL-10 could be secreted throughout the first six weeks of infection and could inhibit monocytes from DPI 35.
authors.affiliation: Unité associée INRA/ENVN Interactions Hôte-Parasite-Milieu, Ecole Nationale Vétérinaire de Nantes. moreau@vet-nantes.fr
authors.forename: E
authors.initials: E
authors.lastname: Moreau
authors.forename: A
authors.initials: A
authors.lastname: Chauvin
authors.forename: C
authors.initials: C
authors.lastname: Boulard
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005210:Fasciola hepatica / Q000276:immunology; D005211:Fascioliasis / Q000276:immunology / Q000662:veterinary*; D007371:Interferon-gamma / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008099:Liver; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D011897:Random Allocation; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
chemical_list: D016753:Interleukin-10; D007371:Interferon-gamma