Endothelium-dependent vasodilatation in Sprague-Dawley rats with postinfarction hypertrophy: lack of endothelial dysfunction in vitro.
The hypothesis was tested whether postinfarction hypertrophy/congestive heart failure in rats is associated with endothelial dysfunction and increased vascular generation of reduced oxygen species. Myocardial infarction was induced in Sprague-Dawley rats by ligation of the left coronary artery. After 16 weeks, endothelium-dependent (with acetylcholine) and -independent (with sodium nitro-prusside) relaxations were studied in isolated aortic rings, and isolated rings from the femoral and mesenteric arteries. The generation of superoxide, hydrogenperoxide, and peroxynitrite was measured in arteries using lucigenin- and luminol-enhanced chemiluminescence techniques. Systolic blood pressure decreased over the 16 week study period as compared to shamoperated control rats; organ weights (lungs, right and left ventricles) significantly increased in coronary artery ligated rats indicating development of congestive heart failure. Surprisingly, concentration response curves with acetylcholine and sodium nitroprusside were almost identical in myocardial infarction rats as compared to control animals, irrespective of which type of vessel was studied (aorta, femoral or mesenteric arteries). In addition, no differences in the production of reduced radical species were found in aortic tissue from heart failure rats as compared to control rats.
Brandes
R P
RP
Department of Internal Medicine, Hannover Medical School, Germany.
Walles
T
T
Koddenberg
G
G
Gwinner
W
W
Mügge
A
A
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D006332:Cardiomegaly / Q000209:etiology / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000502:physiology*; D006333:Heart Failure / Q000209:etiology; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D009569:Nitric Oxide / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013481:Superoxides / Q000378:metabolism; D014664:Vasodilation / Q000187:drug effects
D013481:Superoxides; D009569:Nitric Oxide; D000109:Acetylcholine