title: Protein engineering of ribonucleases.
abstract: Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric RNases display selective toxicity for malignant cells, which is absent in the monomeric mutants.
authors.affiliation: Dipartimento di Chimica Organica e Biologica, Università di Napoli Federico II, Naples, Italy.
authors.forename: V
authors.initials: V
authors.lastname: Cafaro
authors.forename: A
authors.initials: A
authors.lastname: Bracale
authors.forename: F
authors.initials: F
authors.lastname: Formiggini
authors.forename: E
authors.initials: E
authors.lastname: Notomista
authors.forename: G
authors.initials: G
authors.lastname: D'Alessio
authors.forename: A
authors.initials: A
authors.lastname: Di Donato
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry; D002417:Cattle; D008958:Models, Molecular; D009154:Mutation; D011994:Recombinant Proteins / Q000737:chemistry; D012259:Ribonuclease, Pancreatic / Q000737:chemistry* / Q000235:genetics / Q000502:physiology; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D012259:Ribonuclease, Pancreatic