title: A new interference-free lysine biosensor using a non-conducting polymer film.
abstract: An electrochemical biosensor for the determination of lysine to be used for rapid evaluation of food quality has been developed. Platinum electrodes have been coated by electropolymerisation with 1,2-diaminobenzene (1.2-DAB) using cyclic voltammetry. The reduction in the oxidation of interferents compared with the bare platinum electrode was 100% for ascorbic acid, 99% for acetaminophen and 99% for cysteine. The enzyme L-lysine-alpha-oxidase was then immobilised onto the polymer layer by passive adsorption and a calibration curve for lysine constructed. This gave a linear range of 1 x 10(-5) mol/l to 1 x 10(-3) mol/l and a limit of detection of 2 x 10(-7) mol/l.
authors.affiliation: Centro C.N.R. di Studio per l'Elettrochimica e la Chimica Fisica delle Interfasi, Rome, Italy. curulli@axrma.uniromal.it
authors.forename: S
authors.initials: S
authors.lastname: Kelly
authors.forename: A
authors.initials: A
authors.lastname: Curulli
authors.forename: C
authors.initials: C
authors.lastname: O'Sullivan
authors.forename: G G
authors.initials: GG
authors.lastname: Guilbault
authors.forename: G
authors.initials: G
authors.lastname: Palleschi
mesh_terms: D015374:Biosensing Techniques; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis*
chemical_list: D004800:Enzymes, Immobilized; D008239:Lysine