Host genes affecting survival period of chemically induced bladder cancer in mice.
Treatment of C57BL/6 J (B6) and NON male mice with N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) resulted in a high incidence of bladder cancer. The mean survival period, however, differed significantly by strain: 481+/-219 days in B6 (n = 31) and 203+/-119 days in NON (n = 30) (P < 0.0001). Major causes of death were renal failure due to obstruction of the urinary tract, or local invasion of tumors. The fact that the BBN-treated NON x B6 reciprocal F1 mice had survival periods as short as those of the parental NON mice suggests a genetically dominant susceptibility in NON or recessive resistance in B6. A linkage analysis of 248 back-cross mice to B6 suggested at least two quantitative trait loci determining the length of the survival period: one was mapped close to D2Mit260 (logarithm of odds, LOD, score 2.21), a microsatellite marker locus 83 cM from the centromere on chromosome 2, and another was close to D6Mit159, 7 cM from the centromere on chromosome 6 (LOD score 2.51).
Higashi
S
S
Department of Pathology and Biology of Disease, Graduate School of Medicine, Kyoto University, Japan.
Murai
T
T
Mori
S
S
Kamoto
T
T
Yoshitomi
M
M
Arakawa
Y
Y
Makino
S
S
Fukushima
S
S
Yoshida
O
O
Hiai
H
H
D000818:Animals; D002085:Butylhydroxybutylnitrosamine / Q000494:pharmacology; D002874:Chromosome Mapping; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D018895:Microsatellite Repeats; D019655:Quantitative Trait, Heritable; D016019:Survival Analysis; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000235:genetics* / Q000401:mortality
D002085:Butylhydroxybutylnitrosamine