Evaluation of equine infectious anemia virus core proteins produced in a baculovirus expression system in agar gel immunodiffusion test and enzyme-linked immunosorbent assay.
Equine infectious anemia virus (EIAV) core proteins (Gag and p26) obtained from a baculovirus expression system were used in agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) antigens to test seventy-six horse sera. Those sera showed false-positive reaction in AGID test using Nisseiken antigen. However, none of them showed false-positive reaction with both of the expressed antigens. The 76 horse sera were also tested by ELISA. The sera gave a high background in ELISA using Nisseiken antigen. Gag and p26 reacted strongly against positive sera from horses immunized with Nisseiken antigen.
Kong
X G
XG
National Institute of Animal Health, Ibaraki, Japan.
Pang
H
H
Sugiura
T
T
Matsumoto
Y
Y
Onodera
T
T
Akashi
H
H
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D016367:Baculoviridae; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004859:Equine Infectious Anemia / Q000175:diagnosis*; D015683:Gene Products, gag / Q000276:immunology*; D006736:Horses; D005779:Immunodiffusion / Q000662:veterinary; D004860:Infectious Anemia Virus, Equine / Q000276:immunology*; D018411:Spodoptera; D014758:Viral Core Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D015683:Gene Products, gag; D014758:Viral Core Proteins; C117895:equine infectious anemia virus, p26