title: Manifestations and mechanisms of radiation-induced genomic instability in V-79 Chinese hamster cells.
abstract: OBJECTIVE
  The relationship between different forms of persistent radiation damage in irradiated cells was investigated in order to identify a common underlying mechanism.
  
  
  METHODS
  V-79 Chinese hamster cells were irradiated with different doses of X-rays, neutrons and alpha-particles. In the progeny of surviving cells, up to 4 weeks after irradiation, delayed reproductive death, delayed micronuclei, delayed appearance of dicentric chromosomes and delayed apoptosis were investigated in parallel.
  
  
  RESULTS
  A similar dose-response relationship was found for all endpoints, with a steep rise at low doses to a plateau at doses > 3 Gy. The target for inducing genomic instability by alpha-particles is larger than the nucleus. All chromosomes are equally involved in delayed breakage reunion events.
  
  
  CONCLUSIONS
  The results indicate that non-lethal radiation damage to an extranuclear target leads to a persistent increase in clastogenic activity in the surviving irradiated cells.
authors.affiliation: Department of Radiation Biology, St Bartholomew's and the Royal London School of Medicine and Dentistry, UK. k.r.trott@mds.qmw.ac.uk
authors.forename: K R
authors.initials: KR
authors.lastname: Trott
authors.forename: M
authors.initials: M
authors.lastname: Jamali
authors.forename: L
authors.initials: L
authors.lastname: Manti
authors.forename: A
authors.initials: A
authors.lastname: Teibe
mesh_terms: D000512:Alpha Particles / Q000009:adverse effects; D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D016466:CHO Cells / Q000528:radiation effects*; D002470:Cell Survival / Q000528:radiation effects; D002869:Chromosome Aberrations / Q000235:genetics; D002999:Clone Cells / Q000528:radiation effects; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D005796:Genes / Q000528:radiation effects*; D048629:Micronuclei, Chromosome-Defective / Q000528:radiation effects; D009502:Neutrons / Q000009:adverse effects; D011839:Radiation, Ionizing; D013997:Time Factors; D014965:X-Rays / Q000009:adverse effects