title: Copper(II) complexation properties and surfactant activity of 3-[N, N-bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid and N-(2-Hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid pH buffers which may affect trace metal speciation in in vitro studies.
abstract: Disadvantages of the some zwitterionic pH buffers are (i) that they can interact with metal ions as well as protons, and (ii) that they may have a surfactant effect in chemical or in vitro biological or biochemical studies. This has to be taken into account when a buffer is selected. Here, the copper-complexing capacity and the surfactant activity of three compounds, 3-[N,N-bis (2-hydroxyethyl)amino]-2-hydroxypropanesulfonic acid (DIPSO), N-(2-hydroxyethyl)piperazine-N'-(2-hydroxypropanesulfonic acid) (HEPPSO), and piperazine-N,N'-bis(2-ethanesulfonic acid) (Pipes), were investigated. Global stability constants (log betaabc) of copper(II)-buffer complexes were determined, at 25 degrees C, 0.5 M ionic strength, and at 0.8 mM buffer concentration, by pH and pCu ion-selective electrode measurements. Here, betaabc corresponds to the equilibrium: aCu2+ + bL- + cH+ left and right arrow (CuaLbHc)(2a-b+c); HL = DIPSO or HEPPSO; c: +1 = proton; -1 = hydroxide. Using SUPERQUAD constants were calculated, giving: DIPSO: log beta110 = 5.02, log beta120 = 8.99, log beta130 = 13.0, log beta140 = 16.3, log beta14-1 = 9.26, log beta14-2 = 0.645, log beta150 = 20.5, log beta160 = 24.3, log beta16-1 = 16.1, log beta16-2 = 8.98; HEPPSO: log beta110 = 4.29, log beta120 = 8.35, log beta130 = 12.1, log beta140 = 15.9, log beta150 = 19.6, log beta160 = 23.4, log beta16-1 = 14.9. The pKa values were determined at higher buffer concentrations, giving a value 7.33 for DIPSO and 7.84 for HEPPSO at 2.0 mM buffer concentration. Effects of buffer concentration on stability and acidity constants were investigated and compared with measurements using voltammetric and potentiometric stripping analysis, confirming no copper(II) complexation by Pipes. Surfactant activities were determined using alternating current polarography, confirming marked surface activity of 10 mM of DIPSO or HEPPSO.
authors.affiliation: Departamento de Química, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre 687, Porto, P4150, Portugal. mtvascon@fc.up.pt
authors.forename: M T
authors.initials: MT
authors.lastname: Vasconcelos
authors.forename: M A
authors.initials: MA
authors.lastname: Azenha
authors.forename: C M
authors.initials: CM
authors.lastname: Almeida
mesh_terms: D002021:Buffers; D003300:Copper / Q000737:chemistry*; D004983:Ethanolamines / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000032:analysis*; D010879:Piperazines / Q000737:chemistry*; D013451:Sulfonic Acids / Q000737:chemistry*
chemical_list: C117127:3-(N,N-bis(2-hydroxyethyl)amino)-2-hydroxypropanesulfonic acid; D002021:Buffers; D004983:Ethanolamines; D008670:Metals; C117128:N-(2-hydroxyethyl)piperazine-N'-2-hydroxypropanesulfonic acid; D010879:Piperazines; D013451:Sulfonic Acids; D003300:Copper