Downregulation of mRNA expression of Edg-3, a putative sphingosine 1-phosphate receptor coupled to Ca2+ signaling, during differentiation of HL-60 leukemia cells.
We measured the mRNA expression of the recently identified putative sphingosine 1-phosphate (S1P) receptors, i.e., Edg-1, AGR16/H218, and Edg-3, in HL-60 leukemia cells. Of these putative receptors, Edg-3 mRNA was abundantly expressed in undifferentiated HL-60 cells. Further, its mRNA expression was markedly downregulated by inducers of cell differentiation such as dibutyryl cAMP, retinoic acid, and 1alpha, 25-dihydroxyvitamin D3. The reduction of mRNA expression was associated with the attenuation of an S1P-induced increase in cytoplasmic free Ca2+ concentration. Thus, Edg-3, whose mRNA expression is downregulated during cell differentiation, may be responsible for the S1P-induced Ca2+ response in HL-60 leukemia cells.
Sato
K
K
Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.
Murata
N
N
Kon
J
J
Tomura
H
H
Nochi
H
H
Tamoto
K
K
Osada
M
M
Ohta
H
H
Tokumitsu
Y
Y
Ui
M
M
Okajima
F
F
D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D018922:HL-60 Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020672:I-kappa B Proteins; D008246:Lysophospholipids; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism
D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D008246:Lysophospholipids; C000606204:NFKBIA protein, human; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D000072000:NF-KappaB Inhibitor alpha; C060506:sphingosine 1-phosphate; D013110:Sphingosine; D002118:Calcium