title: Vitelline envelope genes of the yellow fever mosquito, Aedes aegypti.
abstract: Vitelline envelope genes from the mosquito Aedes aegypti were analyzed with respect to their DNA sequences, genomic representation, temporal and spatial expression profiles and response to 20-hydroxyecdysone. Genomic clones of three vitelline envelope genes, 15a-1, 15a-2 and 15a-3 were isolated. Southern analysis indicates that all three genes are represented by a single copy in the genome. The deduced amino acid sequences of all three vitelline envelope genes contain a conserved region of 46 residues that overlaps with a region that is conserved in four Drosophila melanogaster vitelline envelope genes. DNA was sequenced flanking the 15a-1, 15a-2 and 15a-3 coding regions. A 360 bp sequence 5' of the 15a-2 coding region was identified with 72% identity to a sequence upstream of the Ae. aegypti VgA1 vitellogenin gene. The temporal patterns of 15a-1, 15a-2 and 15a-3 expression, as determined by Northern analysis, were similar. The spatial patterns of expression, as determined by whole-mount in situ hybridization, differed between the three genes. 15a-1 and 15a-3 were only expressed in the middle and posterior regions of the follicle, while 15a-2 was also expressed at the anterior region. Vitelline envelope gene expression was higher in ovaries that were dissected at 0, 2 and 10 h following a blood meal and then incubated in vitro for 10 h in medium containing 10(-5) M 20-hydroxyecdysone, compared to ovaries that were incubated without hormone.
authors.affiliation: Department of Genetics, Case Western Reserve University, Cleveland, OH 44106, USA. mje8@po.cwru.edu
authors.forename: M J
authors.initials: MJ
authors.lastname: Edwards
authors.forename: D W
authors.initials: DW
authors.lastname: Severson
authors.forename: H H
authors.initials: HH
authors.lastname: Hagedorn
mesh_terms: D000330:Aedes / Q000235:genetics*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004441:Ecdysterone / Q000494:pharmacology; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008040:Genetic Linkage; D019476:Insect Proteins / Q000235:genetics*; D007303:Insect Vectors / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010053:Ovary / Q000187:drug effects; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014018:Tissue Distribution; D014158:Transcription, Genetic; D014817:Vitelline Membrane; D015004:Yellow Fever
chemical_list: D018076:DNA, Complementary; D004527:Egg Proteins; D019476:Insect Proteins; D008565:Membrane Proteins; C035651:vitelline membrane proteins; D004441:Ecdysterone