title: Expression of matrix metalloproteinases in human glioma cell lines in the presence of IL-10.
abstract: Matrix metalloproteinases have been implicated to play a vital role in glioma invasion as they degrade extracellular matrix to facilitate the subsequent migration of tumor cells into the surrounding brain tissue. The cytokine Interleukin-10 (IL-10) was detected recently in glial tumors in vivo. Expression of specific IL-10 mRNA as well as blood serum levels of IL-10 in glioma patients increased with malignancy suggesting a functional role of IL-10 in glioma progression. Moreover, glioma cell migration in vitro was enhanced in the presence of IL-10. We therefore investigated the expression of the matrix metalloproteinases (MMPs) stromelysin-1 (MMP-3), 72-kDa collagenase (MMP-2), 92-kDa collagenase (MMP-9), matrilysin (MMP-7) and the human macrophage metalloelastase (MMP-12). In addition, a possible relation between exposure of glioma cells to IL-10 and invasiveness of these cells due to MMP expression was analyzed. Experiments with Matrigel coated Boyden chambers revealed a pronounced dose dependent effect of IL-10 on glioma invasiveness. The synthetic MMP-inhibitor Marimastat markedly reduced cell invasion in the Boyden chambers confirming the significance of MMPs in the process of invasion. Subsequently, the expression level of MMPs and the serine protease uPA was investigated in 7 glioma cell lines (U373, GaMG, U251, GHE, SNB19, U138 and D54) by RT-PCR. In all but one cell line no enhancement of MMP expression by IL-10 was detected. Matrilysin in U373 cells was the only protease found to be upregulated in the presence of IL-10 dependent on cell density. The present data suggest that IL-10 related effects on the invasive properties of the cell lines are not directly mediated by an upregulation of matrix metalloproteinase expression.
authors.affiliation: Department of Neurosurgery, University of Wuerzburg, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Wagner
authors.forename: C
authors.initials: C
authors.lastname: Stegen
authors.forename: H
authors.initials: H
authors.lastname: Bouterfa
authors.forename: C
authors.initials: C
authors.lastname: Huettner
authors.forename: S
authors.initials: S
authors.lastname: Kerkau
authors.forename: W
authors.initials: W
authors.lastname: Roggendorf
authors.forename: K
authors.initials: K
authors.lastname: Roosen
authors.forename: J C
authors.initials: JC
authors.lastname: Tonn
mesh_terms: D001932:Brain Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D002460:Cell Line; D010450:Endopeptidases / Q000096:biosynthesis / Q000302:isolation & purification; D005109:Extracellular Matrix / Q000187:drug effects / Q000201:enzymology*; D005910:Glioma / Q000188:drug therapy / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D016753:Interleukin-10 / Q000494:pharmacology*; D008666:Metalloendopeptidases / Q000096:biosynthesis / Q000378:metabolism*; D012333:RNA, Messenger / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D011994:Recombinant Proteins / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011994:Recombinant Proteins; D016753:Interleukin-10; D010450:Endopeptidases; D008666:Metalloendopeptidases