title: Evidence for a common epitope between bovine alpha-lactalbumin and beta-lactoglobulin.
abstract: The aim of this work was to check and quantify any cross-reactivities among the main bovine whey proteins, utilizing purified polyclonal antibodies against bovine beta-lactoglobulin, alpha-lactalbumin and serum albumin, and to identify possible common epitope(s). Purified polyclonal anti-bovine beta-lactoglobulin antibodies show 10% cross-reactivity with bovine alpha-lactalbumin, both in its native and its denatured form. A continuous stretch of four amino acids common to alpha-lactalbumin and beta-lactoglobulin that might be responsible for this cross-reactivity has been identified. Cross-reactivity between this antibody and bovine serum albumin is, on the contrary, negligible. Purified polyclonal anti-bovine alpha-lactalbumin and anti-serum albumin antibodies do not cross-react.
authors.affiliation: Centro Studi Alimentazione Animali, CNR, Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Baroglio
authors.forename: M G
authors.initials: MG
authors.lastname: Giuffrida
authors.forename: A
authors.initials: A
authors.lastname: Cantisani
authors.forename: L
authors.initials: L
authors.lastname: Napolitano
authors.forename: E
authors.initials: E
authors.lastname: Bertino
authors.forename: C
authors.initials: C
authors.lastname: Fabris
authors.forename: A
authors.initials: A
authors.lastname: Conti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology*; D007768:Lactalbumin / Q000737:chemistry / Q000276:immunology*; D007782:Lactoglobulins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000939:Epitopes; D007782:Lactoglobulins; D007768:Lactalbumin