title: Changes in rat paw perfusion after experimental mononeuropathy: assessment by laser Doppler fluxmetry.
abstract: This study was performed to determine the changes in perfusion that occur after chronic constriction injury (CCI). Male Sprague-Dawley rats weighing 275-300 g had loosely constricting ligatures placed around the left sciatic nerve. Paw withdrawal latency (PWL) to heat, skin temperature, and skin perfusion (laser Doppler) of the hind paws were measured before and for 30 days after CCI. PWL decreased significantly on the side of the CCI (maximum of 34% decrease on Postoperative Day [POD] 3), then returned to normal over a 20-day period. Skin temperature initially increased on the side of CCI, then decreased with respect to the control limb on PODs 20-30. Despite the initial increase in skin temperature on the side of CCI, skin perfusion significantly decreased immediately after CCI (maximum of 51% decrease on POD 6). The perfusion gradually returned to normal over 20 days. Because return to normal perfusion occurred while the skin temperature became colder than the control side, we conclude that there is no relationship between paw surface temperature and perfusion.
  
  
  CONCLUSIONS
  Our data suggest that loss of sympathetic tone in thermoregulatory arteriovenous anastomoses leads to decreased nutritional blood flow to the skin of the affected limb after chronic constriction injury, which is consistent with the findings reported in humans with reflex sympathetic dystrophy.
authors.affiliation: Department of Anesthesiology, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
authors.forename: A H
authors.initials: AH
authors.lastname: Hord
authors.forename: D D
authors.initials: DD
authors.lastname: Denson
authors.forename: M J
authors.initials: MJ
authors.lastname: Huerkamp
authors.forename: J G
authors.initials: JG
authors.lastname: Seiler
mesh_terms: D000818:Animals; D005528:Foot / Q000098:blood supply* / Q000294:innervation*; D006614:Hindlimb / Q000098:blood supply / Q000294:innervation; D007103:Immobilization; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008026:Ligation; D008297:Male; D010147:Pain Measurement; D010477:Perfusion; D010523:Peripheral Nervous System Diseases / Q000209:etiology / Q000503:physiopathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology