title: Culture-amplified detection of dengue virus from serum in an outbreak of dengue fever.
abstract: An outbreak of dengue type 2 occurred in North Queensland, Australia, between December 1996 and April 1997. Culture of serum in the Aedes albopictus C6/36 cell line with detection using immunofluorescent staining was compared with a culture-amplified detection system using an immunoperoxidase staining method in a microtiter plate format. A total of 374 serum specimens from individuals during the outbreak were tested. Ninety-five specimens were positive using immunofluorescence and ninety-two were positive using the immunoperoxidase method (sensitivity 91.6%; specificity 98.2%). The immunoperoxidase method is quicker, easier to perform, and does not require the use of an immunofluorescent microscope. The method is more suited to the processing of large numbers of specimens in an outbreak and could be used in endemic areas with limited virological resources.
authors.affiliation: Department of Pathology, Townsville General Hospital, Queensland, Australia.
authors.forename: F
authors.initials: F
authors.lastname: Gleeson
authors.forename: J
authors.initials: J
authors.lastname: McBride
authors.forename: R
authors.initials: R
authors.lastname: Norton
mesh_terms: D000328:Adult; D000330:Aedes; D000818:Animals; D002460:Cell Line; D002648:Child; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus / Q000302:isolation & purification*; D004196:Disease Outbreaks; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D007124:Immunoenzyme Techniques; D011793:Queensland; D012680:Sensitivity and Specificity; D013997:Time Factors; D010948:Viral Plaque Assay; D014773:Virology / Q000379:methods*