title: Natural interferon enhances expression of placental alkaline phosphatase in human seminoma xenograft.
abstract: The purpose of this study was to investigate the effect of natural interferon (IFN) on the expression of placental alkaline phosphatase (PLAP) in a human seminoma xenograft in severe combined immunodeficient mice. Mice were injected intramuscularly with 3x10(5) U/mouse of IFN, twice a day, for five consecutive days. A significant increase in PLAP level of the xenografts followed IFN treatment. A radiolabeled anti-PLAP monoclonal antibody (MAb) was intravenously injected on the first day of IFN administration in order to determine if IFN has the potential to enhance the efficacy of an anti-PLAP MAb in the detection of seminoma. Enhanced retention of the anti-PLAP MAb was observed at 7 and 11 days after MAb administration. Thus, IFN treatment appears to have some effect on the efficacy of the anti-PLAP MAb in the detection of seminoma xenografts.
authors.affiliation: Department of Urology, School of Medicine, Kanazawa University, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Imao
authors.forename: K
authors.initials: K
authors.lastname: Koshida
authors.forename: H
authors.initials: H
authors.lastname: Konaka
authors.forename: T
authors.initials: T
authors.lastname: Uchibayashi
authors.forename: K
authors.initials: K
authors.lastname: Yokoyama
authors.forename: K
authors.initials: K
authors.lastname: Hirano
authors.forename: M
authors.initials: M
authors.lastname: Namiki
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D000911:Antibodies, Monoclonal; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D007457:Iodine Radioisotopes; D007527:Isoenzymes / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D018239:Seminoma / Q000000981:diagnostic imaging / Q000201:enzymology*; D013736:Testicular Neoplasms / Q000000981:diagnostic imaging / Q000201:enzymology*; D013737:Testis / Q000201:enzymology; D014183:Transplantation, Heterologous
chemical_list: D000911:Antibodies, Monoclonal; D007457:Iodine Radioisotopes; D007527:Isoenzymes; C055791:germ-cell AP isoenzyme; D007372:Interferons; D000469:Alkaline Phosphatase