Poly(ethylene glycol)-block-poly(L-lysine) dendrimer: novel linear polymer/dendrimer block copolymer forming a spherical water-soluble polyionic complex with DNA.
Methoxypoly(ethylene glycol)-block-poly(L-lysine) dendrimer was designed to form a water-soluble complex with plasmid DNA. The copolymer was synthesized by the liquid-phase peptide synthesis method. It was characterized by 1H NMR and matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrum. Agarose gel electrophoresis and DNase I protection assay proved that this linear polymer/dendrimer block copolymer assembled spontaneously with plasmid DNA, forming a water-soluble complex which increased the stability of the complexed DNA. Atomic force microscopy of the complex was evaluated at various charge ratios showing that the copolymer/DNA complex was like a globular shape.
Choi
J S
JS
Department of Chemistry, Seoul National University, San 56-1, Shillim-dong, Kwanak-ku, Seoul, 151-742, Korea.
Lee
E J
EJ
Choi
Y H
YH
Jeong
Y J
YJ
Park
J S
JS
D004247:DNA / Q000737:chemistry*; D003850:Deoxyribonuclease I / Q000037:antagonists & inhibitors; D004587:Electrophoresis, Agar Gel; D009682:Magnetic Resonance Spectroscopy; D018625:Microscopy, Atomic Force; D010957:Plasmids; D011092:Polyethylene Glycols / Q000138:chemical synthesis* / Q000494:pharmacology; D011107:Polylysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014867:Water
C117924:mPEG-block-PLL; D014867:Water; D011107:Polylysine; D011092:Polyethylene Glycols; D004247:DNA; D003850:Deoxyribonuclease I