New multidimensional editing experiments for measurement of amide deuterium isotope effects on Cbeta chemical shifts in 13C, 15N-labeled proteins.
Novel multidimensional NMR pulse sequences for measurement of the three- and four-bond amide deuterium isotope effect on the chemical shifts of 13Cbeta in proteins are presented. The sequences result in editing into two subspectra of a heteronuclear triple resonance spectrum ¿omega(N), omega(Cbeta), omega(Halpha)¿ according to there being a deuterium or a proton attached to 15N for the pertinent correlations. The new experiments are demonstrated by an application to the first module of the 13C,15N-labeled protein RAP 18-112 (N-terminal module of alpha2-macroglobulin receptor associated protein).
Meissner
A
A
Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, Valby, DK-2500, Denmark.
Sørensen
O W
OW
D000577:Amides; D002244:Carbon / Q000737:chemistry*; D002247:Carbon Isotopes; D002352:Carrier Proteins / Q000737:chemistry*; D003903:Deuterium; D006023:Glycoproteins / Q000737:chemistry*; D026563:LDL-Receptor Related Protein-Associated Protein; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018832:Molecular Chaperones / Q000737:chemistry*; D015394:Molecular Structure; D009587:Nitrogen Isotopes
D000577:Amides; D002247:Carbon Isotopes; D002352:Carrier Proteins; D006023:Glycoproteins; D026563:LDL-Receptor Related Protein-Associated Protein; D018832:Molecular Chaperones; D009587:Nitrogen Isotopes; D002244:Carbon; D003903:Deuterium