title: Immunohistochemical distribution of RGS7 protein and cellular selectivity in colocalizing with Galphaq proteins in the adult rat brain.
abstract: Regulators of G protein signaling (RGS) proteins serve as potent GTPase-activating proteins for the heterotrimeric G proteins alphai/o and aq/11. This study describes the immunohistochemical distribution of RGS7 throughout the adult rat brain and its cellular colocalization with Galphaq/11, an important G protein-coupled receptor signal transducer for phospholipase Cbeta-mediated activity. In general, both RGS7 and Galphaq/11 displayed a heterogeneous and overlapping regional distribution. RGS7 immunoreactivity was observed in cortical layers I-VI, being most intense in the neuropil of layer I. In the hippocampal formation, RGS7 immunoreactivity was concentrated in the strata oriens, strata radiatum, mossy fibers, and polymorphic cells, with faint to nondetectable immunolabeling within the dentate gyrus granule cells and CA1-CA3 subfield pyramidal cells. Numerous diencephalic and brainstem nuclei also displayed dense RGS7 immunostaining. Dual immunofluorescence labeling studies with the two protein-specific antibodies indicated a cellular selectivity in the colocalization between RGS7 and Galphaq/11 within many discrete brain regions, such as the superficial cortical layer I, hilus area of the hippocampal formation, and cerebellar Golgi cells. To assess the ability of Galphaq/11-mediated signaling pathways to modulate dynamically RGS expression, primary cortical neuronal cultures were incubated with phorbol 12,13-dibutyrate, a selective protein kinase C activator. A time-dependent increase in levels of mRNA for RGS7, but not RGS4, was observed. Our results provide novel information on the region- and cell-specific pattern of distribution of RGS7 with the transmembrane signal transducer, Galphaq/11. We also describe a possible RGS7-selective neuronal feedback adaptation on Galphaq/11-mediated pathway function, which may play an important role in signaling specificity in the brain.
authors.affiliation: CNS Disorders, Wyeth-Ayerst Research, Princeton, New Jersey 08543-8000, USA.
authors.forename: X Z
authors.initials: XZ
authors.lastname: Khawaja
authors.forename: J J
authors.initials: JJ
authors.lastname: Liang
authors.forename: J A
authors.initials: JA
authors.lastname: Saugstad
authors.forename: P G
authors.initials: PG
authors.lastname: Jones
authors.forename: S
authors.initials: S
authors.lastname: Harnish
authors.forename: P J
authors.initials: PJ
authors.lastname: Conn
authors.forename: M I
authors.initials: MI
authors.lastname: Cockett
mesh_terms: D000367:Age Factors; D000818:Animals; D000906:Antibodies; D015153:Blotting, Western; D001923:Brain Chemistry / Q000502:physiology*; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D005246:Feedback / Q000502:physiology; D005260:Female; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D018613:Microscopy, Confocal; D009474:Neurons / Q000737:chemistry* / Q000502:physiology; D015240:Phorbol 12,13-Dibutyrate / Q000494:pharmacology; D011247:Pregnancy; D011506:Proteins / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D020710:RGS Proteins; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology
chemical_list: D000906:Antibodies; D002273:Carcinogens; D011506:Proteins; D020710:RGS Proteins; D012333:RNA, Messenger; C115771:Rgs7 protein, rat; D015240:Phorbol 12,13-Dibutyrate; D019204:GTP-Binding Proteins