Selective detection and differentiation of subgenus B adenoviruses (types 3, 7 and 11) by polymerase chain reaction.
Application of the polymerase chain reaction (PCR) method for detection of subgenus B adenoviruses (types 3, 7 and 11) was investigated. It is based on a simple (nonnested) PCR using primer pairs specific for the hexon-coding region. The PCR allowed amplification of DNA from subgenus B adenovirus prototype strains (types 3, 7 and 11) and adenovirus isolates (types 3 and 7), whereas it did not amplify DNA from subgenus A (type 31), C (types 1, 2, 5 and 6), D (types 8, 19 and 37), E (type 4) and adenovirus isolates (types 1, 2, 5 and 6). These results suggest that subgenus B adenoviruses (types 3, 7 and 11) are detectable selectively by means of PCR with primer pairs developed in this study. Amplified fragments from adenovirus types 3, 7 and 11 could be differentiated with restriction endonuclease analysis with Rsa I.
Fujimoto
T
T
Division of Microbiology, Hyogo Prefectural Institute of Public Health, Kobe, Japan.
Chikahira
M
M
Nishio
O
O
D000256:Adenoviridae / Q000145:classification / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis; D015252:Deoxyribonucleases, Type II Site-Specific; D016133:Polymerase Chain Reaction / Q000379:methods*; D015183:Restriction Mapping; D012703:Serotyping
D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; C040158:GTAC-specific type II deoxyribonucleases