title: Ouabain- and central sodium-induced hypertension depend on the ventral anteroventral third ventricle region.
abstract: To examine the role of the ventral anteroventral third ventricle (vAV3V) in the hypertension induced by chronic subcutaneous ouabain and intracerebroventricular hypertonic saline, neurons in this area were destroyed by microinjection of an excitotoxin, ibotenic acid. Sham-operated or lesioned Wistar rats were administered ouabain (50 microgram/day) or placebo for 3 wk from subcutaneously implanted controlled release pellets or artificial cerebrospinal fluid (CSF) or CSF containing 0.8 mol/l NaCl (5 microliter/h) infused intracerebroventricularly for 2 wk. At the end of the experiment, mean arterial pressure (MAP) and heart rate at rest and in response to ganglionic blockade by intravenous hexamethonium (30 mg/kg) were assessed. In rats infused with hypertonic saline, responses to air jet stress were also assessed. Baseline MAP in sham-operated rats receiving intracerebroventricular hypertonic saline or subcutaneous ouabain was significantly higher than in control rats (115 +/- 1 vs. 97 +/- 3 and 121 +/- 3 vs. 103 +/- 3 mmHg, respectively). vAV3V lesions abolished the increase in MAP elicited by chronic infusion of hypertonic saline or administration of ouabain. Sham-operated rats treated with hypertonic saline or ouabain exhibited significantly enhanced decreases in MAP to hexamethonium, but lesioned rats did not. Rats infused with hypertonic saline demonstrated enhanced responses to air jet stress that were similar in sham-operated and lesioned rats. These results demonstrate that neurons in the vAV3V are essential for the hypertension induced by intracerebroventricular hypertonic saline and subcutaneous ouabain, possibly by increasing sympathetic tone. Cardiovascular responses to air jet stress appear not to be mediated by the vAV3V.
authors.affiliation: Departments of Cellular and Molecular Medicine and of Medicine, University of Ottawa Heart Institute, University of Ottawa, Ottawa, Ontario, Canada K1Y 4W7.
authors.forename: S J
authors.initials: SJ
authors.lastname: Veerasingham
authors.forename: F H
authors.initials: FH
authors.lastname: Leenen
mesh_terms: D000818:Animals; D002552:Cerebral Ventricles / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D004343:Drug Implants; D018690:Excitatory Amino Acid Agonists / Q000494:pharmacology; D006973:Hypertension / Q000139:chemically induced*; D007051:Ibotenic Acid / Q000494:pharmacology; D007276:Injections, Intraventricular; D008297:Male; D008845:Microinjections; D010042:Ouabain / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D012462:Saline Solution, Hypertonic / Q000008:administration & dosage; D012964:Sodium / Q000008:administration & dosage
chemical_list: D004343:Drug Implants; D018690:Excitatory Amino Acid Agonists; D012462:Saline Solution, Hypertonic; D007051:Ibotenic Acid; D010042:Ouabain; D012964:Sodium