Determination of eight synthetic food colorants in drinks by high-performance ion chromatography.
Eight synthetic food colorants (Amaranth, Brilliant Blue, Indigo Carmine, New Red, Ponceau 4R, Sunset Yellow, Tartrazine, Allura Red) were determined by high-performance ion chromatography on an anion-exchange analytical column with very low hydrophobicity and visible absorbance detection. Gradient elution with hydrochloric acid-acetonitrile effected both the chromatographic separation of these colorants and the on-line clean-up of the analytical column, which was very advantageous for routine analysis. High-performance ion chromatography may be a solution to the chromatographic analysis for some water-soluble, organic analytes with strong hydrophobicity. The method has been applied to the determination of colorants in drinks and in instant drink powder. No time-consuming pretreatment, as used in conventional liquid chromatography, was needed.
Chen
Q C
QC
Research Center for Eco-Environmental Sciences, Academia Sinica, Beijing, China.
Mou
S F
SF
Hou
X P
XP
Riviello
J M
JM
Ni
Z M
ZM
D016477:Artifacts; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D005505:Food Coloring Agents / Q000032:analysis*; D007477:Ions; D013057:Spectrum Analysis
D005505:Food Coloring Agents; D007477:Ions