title: Downregulation of mRNA expression of Edg-3, a putative sphingosine 1-phosphate receptor coupled to Ca2+ signaling, during differentiation of HL-60 leukemia cells.
abstract: We measured the mRNA expression of the recently identified putative sphingosine 1-phosphate (S1P) receptors, i.e., Edg-1, AGR16/H218, and Edg-3, in HL-60 leukemia cells. Of these putative receptors, Edg-3 mRNA was abundantly expressed in undifferentiated HL-60 cells. Further, its mRNA expression was markedly downregulated by inducers of cell differentiation such as dibutyryl cAMP, retinoic acid, and 1alpha, 25-dihydroxyvitamin D3. The reduction of mRNA expression was associated with the attenuation of an S1P-induced increase in cytoplasmic free Ca2+ concentration. Thus, Edg-3, whose mRNA expression is downregulated during cell differentiation, may be responsible for the S1P-induced Ca2+ response in HL-60 leukemia cells.
authors.affiliation: Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Sato
authors.forename: N
authors.initials: N
authors.lastname: Murata
authors.forename: J
authors.initials: J
authors.lastname: Kon
authors.forename: H
authors.initials: H
authors.lastname: Tomura
authors.forename: H
authors.initials: H
authors.lastname: Nochi
authors.forename: K
authors.initials: K
authors.lastname: Tamoto
authors.forename: M
authors.initials: M
authors.lastname: Osada
authors.forename: H
authors.initials: H
authors.lastname: Ohta
authors.forename: Y
authors.initials: Y
authors.lastname: Tokumitsu
authors.forename: M
authors.initials: M
authors.lastname: Ui
authors.forename: F
authors.initials: F
authors.lastname: Okajima
mesh_terms: D016475:3T3 Cells; D000818:Animals; D015152:Blotting, Northern; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics*; D018922:HL-60 Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020672:I-kappa B Proteins; D008246:Lysophospholipids; D051379:Mice; D000072000:NF-KappaB Inhibitor alpha; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D011956:Receptors, Cell Surface / Q000502:physiology*; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D020672:I-kappa B Proteins; D008246:Lysophospholipids; C000606204:NFKBIA protein, human; C000606205:Nfkbia protein, mouse; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D049312:Receptors, Lysophospholipid; D000072000:NF-KappaB Inhibitor alpha; C060506:sphingosine 1-phosphate; D013110:Sphingosine; D002118:Calcium