Detection and sequence analysis of hepatitis B virus integration in peripheral blood mononuclear cells.
A PCR-based technique was used to detect hepatitis B virus (HBV) integration in peripheral blood mononuclear cells from patients with chronic hepatitis B. Integrated HBV DNA sequences, with virus-cell junctions located in the cohesive region between direct repeat 1 (DR1) and DR2, were found in 2 of 10 studied patients.
Laskus
T
T
Division of Gastroenterology and Hepatology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA. Laskus@pitt.edu
Radkowski
M
M
Wang
L F
LF
Nowicki
M
M
Rakela
J
J
D001483:Base Sequence; D004279:DNA, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*; D016662:Virus Integration
D004279:DNA, Viral