Video-enhanced microscopic visualization of apoptotic cell death caused by anti-Fas antibody in living human glioma cells.
To study the morphological changes of anti-Fas antibody-mediated apoptosis in living U251-SP human glioma cells, we employed video-enhanced contrast differential interference contrast (VEC-DIC) microscopy. In our previous study, we investigated the susceptibility of human glioma cell lines to anti-Fas Immunoglobulin M (IgM) antibody. U251-SP cells express Fas antigen on their surface. The cells exposed to anti-Fas antibody underwent apoptotic cell death, as reported previously. In this study, morphological changes of apoptosis characterized by bleb formation, shrinkage of cells, and nuclear condensation were observed under VEC-DIC microscopy in U251-SP human glioma cells treated with anti-Fas antibody. These results demonstrate the usefulness of VEC-DIC microscopy to study the process of apoptotic cell death.
Ohta
S
S
Department of Neurosurgery, Hamamatsu University School of Medicine, Japan.
Yoshida
J
J
Yamamoto
S
S
Uemura
K
K
Wakabayashi
T
T
Mizuno
M
M
Sakurai
T
T
Terakawa
S
S
D000906:Antibodies; D017209:Apoptosis; D005910:Glioma / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D007075:Immunoglobulin M; D018715:Microscopy, Video; D014407:Tumor Cells, Cultured; D019014:fas Receptor
D000906:Antibodies; D007075:Immunoglobulin M; D019014:fas Receptor