Influenza A virus lacking the NS1 gene replicates in interferon-deficient systems.
The NS1 protein is the only nonstructural protein encoded by influenza A virus. It has been proposed that the NS1 performs several regulatory functions during the viral replication cycle, including the regulation of synthesis, transport, splicing, and translation of mRNAs. Through the use of reverse genetics, a viable transfectant influenza A virus (delNS1) which lacks the NS1 gene has been generated. Our results indicate that the NS1 of influenza A virus is an auxiliary (virulence) factor which plays a crucial role in inhibiting interferon-mediated antiviral responses of the host.
García-Sastre
A
A
Department of Microbiology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, New York, 10029, USA.
Egorov
A
A
Matassov
D
D
Brandt
S
S
Levy
D E
DE
Durbin
J E
JE
Palese
P
P
Muster
T
T
D000818:Animals; D002460:Cell Line; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D004285:Dogs; D017353:Gene Deletion; D009980:Influenza A virus / Q000235:genetics* / Q000502:physiology; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009976:Orthomyxoviridae Infections / Q000235:genetics / Q000503:physiopathology*; D050794:STAT1 Transcription Factor; D015398:Signal Transduction; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D014162:Transfection; D014709:Vero Cells; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
D004268:DNA-Binding Proteins; C085820:INS1 protein, influenza virus; D050794:STAT1 Transcription Factor; C494080:Stat1 protein, mouse; D015534:Trans-Activators; D017361:Viral Nonstructural Proteins