[Determination of the expression phase of chb(V40) gene in the cell cycle of Drosophila melanogaster].
Using autoradiography, we have determined cell cycle parameters in neuroblasts of III-rd instar larvae of D. melanogaster. The overall duration of the cell cycle is 9 h, tG1 = 4 h, tS = 3.5 h, tG2 = 1 h, and tM = = 0.5 h. Using histochemical staining for beta-galactosidase activity, we have determined the stage of expression of the reporter gene of P[1ArB] element inserted into chb gene. The chb gene, which codes for a centrosomal protein, is expressed during the last third of S phase. This result illustrates the feasibility of using the enhancer trap P[1ArB] to study differential expression of cell cycle genes.
Trunova
S A
SA
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Science, Novosibirsk, Russia.
Dubatolova
T D
TD
Omel'ianchuk
L V
LV
D000818:Animals; D001345:Autoradiography; D002453:Cell Cycle / Q000235:genetics*; D004331:Drosophila melanogaster / Q000166:cytology / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D017344:Genes, Insect; D017930:Genes, Reporter; D006651:Histocytochemistry; D001616:beta-Galactosidase / Q000235:genetics
D001616:beta-Galactosidase