Control of Herbaspirillum seropedicae NifA activity by ammonium ions and oxygen.
The activity of a truncated form of Herbaspirillum seropedicae NifA in different genetic backgrounds showed that its regulatory domain is involved in nitrogen control but not in O2 sensitivity or Fe dependence. The model for nitrogen control involving PII could thus apply to the proteobacteria at large. NifA may have a role in controlling ADP-ribosylation of nitrogenase in Azospirillum brasilense.
Souza
E M
EM
Department of Biochemistry and Department of Pharmacology, Universidade Federal do Paranà, Curitiba, PR, Brazil. souzaem@bio.ufpr.br
Pedrosa
F O
FO
Drummond
M
M
Rigo
L U
LU
Yates
M G
MG
D000641:Ammonia / Q000494:pharmacology*; D015806:Azospirillum brasilense / Q000201:enzymology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D003676:Deferoxamine / Q000494:pharmacology; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics* / Q000378:metabolism; D010088:Oxidoreductases; D010100:Oxygen / Q000494:pharmacology*; D011401:Promoter Regions, Genetic; D012045:Regulatory Sequences, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D001426:Bacterial Proteins; C061338:NifA protein, Bacteria; D014157:Transcription Factors; D000641:Ammonia; D010088:Oxidoreductases; D009591:Nitrogenase; C029130:nitrogenase reductase; D003676:Deferoxamine; D010100:Oxygen