Mouse Sprr2 genes: a clustered family of genes showing differential expression in epithelial tissues.
Small proline-rich (SPR) proteins are structural components of the cornified cell envelope of stratified squamous epithelia. They are subdivided into three families, i.e., SPR1, SPR2, and SPR3, of which the SPR2 family is the most complex. To understand the significance of this complexity, we have isolated 11 mouse Sprr2 genes, constructed a provisional physical map of the Sprr2 locus on mouse Chromosome 3, and examined the expression patterns of the Sprr2 genes in mouse epithelial tissues. The 11 Sprr2 sequences are highly conserved with a central domain containing a variable number of repeats. In situ hybridization showed the Sprr2 expression to be confined to epithelia. RT-PCR using primers specific for each of the 11 Sprr2 members demonstrated varying degrees of expression among the individual Sprr2 members in different tissues. The correlation between the physical location of the genes in the Sprr2 locus and their expression patterns suggests multiple levels of controlled expression.
Song
H J
HJ
National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20892, USA.
Poy
G
G
Darwiche
N
N
Lichti
U
U
Kuroki
T
T
Steinert
P M
PM
Kartasova
T
T
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D055233:Cornified Envelope Proline-Rich Proteins; D004247:DNA; D004848:Epithelium / Q000378:metabolism*; D000091344:Filaggrin Proteins; D015870:Gene Expression; D008040:Genetic Linkage; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008969:Molecular Sequence Data; D005810:Multigene Family; D011498:Protein Precursors / Q000235:genetics; D011506:Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid
D055233:Cornified Envelope Proline-Rich Proteins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D008565:Membrane Proteins; D011498:Protein Precursors; D011506:Proteins; C064554:loricrin; C032253:involucrin; D004247:DNA