Characterization of the mobilization region of the Zymomonas mobilis ATCC10988 plasmid pZMO3.
The 2.7-kb Zymomonas mobilis ATCC10988 plasmid pZMO3 contains a coding region (ORF1) indispensable for mobilization. A cis-acting 409-bp sequence between ORF2 (C-terminal) and ORF1 (N-terminal) conferred mobilization activity to pUC19, when the product of ORF1 was provided in trans. In this area, two segments showed homology with previously characterized oriT regions.
Afendra
A S
AS
Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.
Vartholomatos
G
G
Arvanitis
N
N
Drainas
C
C
D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D003227:Conjugation, Genetic; D017353:Gene Deletion; D018014:Gene Transfer Techniques; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics; D017355:Zymomonas / Q000235:genetics*
D001426:Bacterial Proteins; D011994:Recombinant Proteins