title: Antibody responses to an immunodominant nonstructural 1 synthetic peptide in patients with dengue fever and dengue hemorrhagic fever.
abstract: Two flaviviruses, dengue (DEN) virus and Japanese encephalitis (JE) virus, are important because of their global distribution and the frequency of epidemics in tropical and subtropical areas. To study the B-cell epitopes of nonstructural 1 (NS1) glycoprotein and anti-NS1 antibody response in DEN infection, a series of 15-mer synthetic peptides from the predicted B-cell linear epitopes of DEN-2 NS1 protein were prepared. Enzyme-linked immunosorbent assay (ELISA) was performed to analyze antibody responses to these peptides from sera of both DEN and JE patients. One peptide derived from DEN-2 NS1, D2 NS1-P1 (amino acids 1-15), was identified as the immunodominant epitope that reacted with sera from dengue fever (DF) patients but not JE patients. The isotype of D2 NS1-P1-specific antibodies was mainly immunoglobulin M (IgM) in all sera that tested positive. A specificity study demonstrated that sera from all four DEN types reacted with D2 NS1-P1. A dynamics study showed that specific antibodies to this peptide could be detected as early as 2 days after the onset of symptoms. We observed significant anti-D2 NS1-P1 antibody responses in 45% of patients with primary and secondary infections with DF or with dengue hemorrhagic fever. This is the first report demonstrating that significant anti-DEN NS1 antibodies can be induced in the sera of patients with primary DEN infection.
authors.affiliation: Division of Epidemiology, National Institute of Preventive Medicine, Department of Health, Taipei, Taiwan.
authors.forename: J H
authors.initials: JH
authors.lastname: Huang
authors.forename: J J
authors.initials: JJ
authors.lastname: Wey
authors.forename: Y C
authors.initials: YC
authors.lastname: Sun
authors.forename: C
authors.initials: C
authors.lastname: Chin
authors.forename: L J
authors.initials: LJ
authors.lastname: Chien
authors.forename: Y C
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000833:Animals, Suckling; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003289:Convalescence; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D004672:Encephalitis, Japanese / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016056:Immunodominant Epitopes / Q000032:analysis / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood; D051379:Mice; D008969:Molecular Sequence Data; D010452:Peptide Biosynthesis / Q000235:genetics; D019595:Severe Dengue / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D016056:Immunodominant Epitopes; D007075:Immunoglobulin M; C079340:NS1 protein, Dengue virus type 2; D014614:Vaccines, Synthetic; D017361:Viral Nonstructural Proteins