Effect of insulin-like growth factor I on in vivo intestinal absorption of D-galactose in cirrhotic rats.
Insulin-like growth factor I (IGF-I) bioavailability is reduced in liver cirrhosis, a condition frequently associated with malnutrition. We have analyzed in vivo absorption of D-galactose by jejunal loops in rats with CCl4-induced liver cirrhosis and the influence of IGF-I on intestinal sugar transport in this disease. Two different study protocols were followed. In protocol 1, healthy control rats or cirrhotic rats received saline or IGF-I (2 micrograms . 100 g body wt-1. day-1) for 2 wk. In protocol 2, control and cirrhotic rats received saline or IGF-I as a bolus injection of 1 microgram/100 g body wt followed by continuous infusion of the same dose for 100 min. In vivo D-galactose absorption was reduced in cirrhotic rats compared with healthy controls. IGF-I, as both a chronic (protocol 1) and acute treatment (protocol 2), was able to improve sugar transport in cirrhotic rats but had no effect on sugar absorption in healthy rats. A significant elongation of enterocyte microvilli was observed in cirrhotic animals; this alteration was totally or partially corrected by chronic or acute IGF-I administration. Our results show that in vivo jejunal sugar transport and microvilli structure are altered in liver cirrhosis and that IGF-I, among other effects, may correct these changes by modulating cytoskeletal organization in enterocytes.
Castilla-Cortázar
I
I
Department of Human Physiology, Liver Unit, School of Medicine, University of Navarra, 31080 Pamplona, Spain.
Picardi
A
A
Tosar
A
A
Ainzúa
J
J
Urdaneta
E
E
García
M
M
Pascual
M
M
Quiroga
J
J
Prieto
J
J
D000818:Animals; D004334:Drug Administration Schedule; D005690:Galactose / Q000493:pharmacokinetics*; D007334:Insulin-Like Growth Factor I / Q000008:administration & dosage / Q000494:pharmacology*; D007408:Intestinal Absorption / Q000187:drug effects*; D007444:Inulin / Q000493:pharmacokinetics; D007583:Jejunum / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism* / Q000473:pathology; D008871:Microvilli / Q000648:ultrastructure; D010539:Permeability; D051381:Rats; D012016:Reference Values; D013997:Time Factors
D007334:Insulin-Like Growth Factor I; D007444:Inulin; D005690:Galactose