title: Regulation of 11beta-hydroxysteroid dehydrogenase type II in rat adrenocortical cells.
abstract: The regulation of 11beta-hydroxysteroid dehydrogenase type II (11beta-HSD2) gene expression was studied in primary cultures of rat adrenocortical cells. The protein kinase A (PKA) pathway agonists forskolin, dibutyryl cAMP and ACTH caused a 5-10 fold increase in 11beta-HSD2 mRNA as determined by semiquantitative PCR. The effect of forskolin could be partially inhibited by the addition of the phorbol ester TPA, an activator of the protein kinase C (PKC) pathway. The increase in mRNA encoding 11beta-HSD2 was accompanied by increased synthesis of 11beta-HSD2 as measured by immunoprecipitation of labeled protein. It is concluded that both the PKA and PKC pathways are involved in the regulation of rat adrenal 11beta-HSD2 gene expression.
authors.affiliation: Department of Physiological Chemistry, University of Medical Sciences, Poznan, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Lecybyl
authors.forename: P P
authors.initials: PP
authors.lastname: Jagodzinski
authors.forename: Z S
authors.initials: ZS
authors.lastname: Krozowski
authors.forename: W H
authors.initials: WH
authors.lastname: Trzeciak
mesh_terms: D042842:11-beta-Hydroxysteroid Dehydrogenases; D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D005260:Female; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D007527:Isoenzymes / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D007527:Isoenzymes; D005576:Colforsin; D006913:Hydroxysteroid Dehydrogenases; D042842:11-beta-Hydroxysteroid Dehydrogenases; D054746:Cyclic AMP-Dependent Protein Kinase Type II; D017868:Cyclic AMP-Dependent Protein Kinases; D013755:Tetradecanoylphorbol Acetate