Protein engineering on enzymes of the peptide elongation cycle in Sulfolobus solfataricus.
The present article is a review of the work done on the elongation factors EF-1 alpha, EF-2 and EF-1 beta isolated from the hyperthermophilic archaeon Sulfolobus solfataricus. The molecular, physical and biochemical properties of the intact, truncated, mutant or chimeric forms are described and compared.
Bocchini
V
V
Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy.
Adinolfi
B S
BS
Arcari
P
P
Arcucci
A
A
Dello Russo
A
A
De Vendittis
E
E
Ianniciello
G
G
Masullo
M
M
Raimo
G
G
D004926:Escherichia coli / Q000737:chemistry; D007700:Kinetics; D009154:Mutation; D020648:Peptide Elongation Factor 1; D020652:Peptide Elongation Factor 2; D010444:Peptide Elongation Factor Tu / Q000737:chemistry; D010445:Peptide Elongation Factors / Q000737:chemistry* / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship; D016944:Sulfolobus / Q000737:chemistry* / Q000201:enzymology
D020648:Peptide Elongation Factor 1; D020652:Peptide Elongation Factor 2; D010445:Peptide Elongation Factors; D011993:Recombinant Fusion Proteins; D010444:Peptide Elongation Factor Tu