title: Comparison of corneal endothelial image analysis by Konan SP8000 noncontact and Bio-Optics Bambi systems.
abstract: OBJECTIVE
  Compare corneal endothelial image analysis by Konan SP8000 and Bio-Optics Bambi image-analysis systems.
  
  
  METHODS
  Corneal endothelial images from 98 individuals (191 eyes), ranging in age from 4 to 87 years, with a normal slit-lamp examination and no history of ocular trauma, intraocular surgery, or intraocular inflammation were obtained by the Konan SP8000 noncontact specular microscope. One observer analyzed these images by using the Konan system and a second observer by using the Bio-Optics Bambi system. Three methods of analyses were used: a fixed-frame method to obtain cell density (for both Konan and Bio-Optics Bambi) and a "dot" (Konan) or "corners" (Bio-Optics Bambi) method to determine morphometric parameters.
  
  
  RESULTS
  The cell density determined by the Konan fixed-frame method was significantly higher (157 cells/mm2) than the Bio-Optics Bambi fixed-frame method determination (p<0.0001). However, the difference in cell density, although still statistically significant, was smaller and reversed comparing the Konan fixed-frame method with both Konan dot and Bio-Optics Bambi comers method (-74 cells/mm2, p<0.0001; -55 cells/mm2, p<0.0001, respectively). Small but statistically significant morphometric analyses differences between Konan and Bio-Optics Bambi were seen: cell density, +19 cells/mm2 (p = 0.03); cell area, -3.0 microm2 (p = 0.008); and coefficient of variation, +1.0 (p = 0.003). There was no statistically significant difference between these two methods in the percentage of six-sided cells detected (p = 0.55).
  
  
  CONCLUSIONS
  Cell densities measured by the Konan fixed-frame method were comparable with Konan and Bio-Optics Bambi's morphometric analysis, but not with the Bio-Optics Bambi fixed-frame method. The two morphometric analyses were comparable with minimal or no differences for the parameters that were studied. The Konan SP8000 endothelial image-analysis system may be useful for large-scale clinical trials determining cell loss; its noncontact system has many clinical benefits (including patient comfort, safety, ease of use, and short procedure time) and provides reliable cell-density calculations.
authors.affiliation: Department of Ophthalmology, Case Western Reserve University and University Hospitals of Cleveland, Ohio 44106-5068, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Benetz
authors.forename: E
authors.initials: E
authors.lastname: Diaconu
authors.forename: S J
authors.initials: SJ
authors.lastname: Bowlin
authors.forename: S S
authors.initials: SS
authors.lastname: Oak
authors.forename: R A
authors.initials: RA
authors.lastname: Laing
authors.forename: J H
authors.initials: JH
authors.lastname: Lass
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D002648:Child; D002675:Child, Preschool; D019781:Corneal Topography / Q000295:instrumentation*; D004728:Endothelium, Corneal / Q000166:cytology*; D006801:Humans; D008875:Middle Aged; D015588:Observer Variation; D012016:Reference Values; D015203:Reproducibility of Results