Identification of the mammalian homologues of the Drosophila timeless gene, Timeless1.
We have identified novel mammalian homologues of a Drosophila clock gene, timeless, and designated them as human TIMELESS1 (hTIM1) and mouse Timeless1 (mTim1), respectively. These genes were mapped by FISH to chromosomal regions 12q12-13 in human and 10D3 in mouse. The deduced amino acid sequences of hTim1 and mTim1 proteins were 1208 and 1197 amino acids in length and shared 83% identity. Northern blot analysis identified a single transcript of 4.5 kb expressed widely in many tissues examined. Unlike the Drosophila counterpart, the levels of the mTim1 transcript exhibited no prominent circadian oscillation in the mouse brain.
Koike
N
N
Human Genome Center, Institute of Medical Science, University of Tokyo, Japan.
Hida
A
A
Numano
R
R
Hirose
M
M
Sakaki
Y
Y
Tei
H
H
D000818:Animals; D001483:Base Sequence; D018797:Cell Cycle Proteins; D002874:Chromosome Mapping; D002881:Chromosomes, Human, Pair 12; D002940:Circadian Rhythm; D003001:Cloning, Molecular; D018076:DNA, Complementary; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins; D006801:Humans; D019476:Insect Proteins / Q000235:genetics*; D047908:Intracellular Signaling Peptides and Proteins; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*
D018797:Cell Cycle Proteins; D018076:DNA, Complementary; D029721:Drosophila Proteins; D019476:Insect Proteins; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; C509769:TIMELESS protein, human; C509770:Timeless protein, mouse; D014157:Transcription Factors; C096445:tim protein, Drosophila