Flow cytometric analysis of Thy-1 expression in myelodysplastic syndrome.
We analyzed the expression of Thy-1 (CD90) antigen on CD34+ bone marrow mononuclear cells (BMMNC) obtained from 25 patients with myelodysplastic syndrome (MDS) by two-color flow cytometry. Five of nine patients (55.6%) with refractory anemia with excess of blasts (RAEB) and two of 16 (12.5%) with RAEB in transformation (RAEB-t) showed more than 20% of Thy-1 expression of their CD34+ BMMNC. Regarding chromosomal abnormalities, -5/5q- or -18 might be correlated with the expression of Thy-1 on CD34+ BMMNC in MDS. Nine patients were analyzed twice, once before and once after leukemic transformation and showed no significant change in Thy-1 expression. These results show that Thy-1 was expressed on CD34+ BMMNC in certain patients with MDS before leukemic transformation and that it was maintained during the disease progression. In contrast, expression of Thy-1 did not seem essential to the leukemic transformation in MDS though the patients with high Thy-1 expression might have poorer prognosis compared with those with low Thy-1 expression.
Inaba
T
T
Second Department of Medicine, Kyoto Prefectural University of Medicine, Japan. inaba178@koto.kpu-m.ac.jp
Shimazaki
C
C
Sumikuma
T
T
Shimura
K
K
Takahashi
R
R
Hirai
H
H
Ashihara
E
E
Sudo
Y
Y
Murakami
S
S
Haruyama
H
H
Fujita
N
N
Yoshimura
M
M
Nakagawa
M
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000754:Anemia, Refractory, with Excess of Blasts / Q000378:metabolism / Q000473:pathology; D018952:Antigens, CD34 / Q000032:analysis / Q000276:immunology; D001854:Bone Marrow Cells / Q000276:immunology / Q000378:metabolism; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D002869:Chromosome Aberrations / Q000473:pathology; D025063:Chromosome Disorders; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008677:Metaphase; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D018800:Thy-1 Antigens / Q000096:biosynthesis*
D018952:Antigens, CD34; D018800:Thy-1 Antigens