title: Analysis of trace atrazine and simazine in environmental samples by liquid chromatography-fluorescence detection with pre-column derivatization reaction.
abstract: Coupled with off-line extraction, a pre-column derivatization liquid chromatographic fluorescence detection (LC-FL) procedure was developed for the determination of atrazine and simazine in soil, crop and water samples. Concentrations in real samples were expected to be at or below the low ng/g level, which requires pre-concentration of analytes and improved detection. 4-(2-Phthalimidyl) benzoyl chloride (PIB-Cl) was used as a pre-column derivation reagent for high-performance liquid chromatography. The clean-up and second-time concentration procedures, which were indispensable in the conventional analytical methods for soil and crop analysis because of the complexity of the samples, were replaced by a derivatization reaction between PIB-Cl and the analytes. The fluorescent and ultraviolet characteristics of the derivatives were investigated. The derivatization reaction and chromatographic separation conditions were optimized systematically. Detection limits of 1.2 ng/g for atrazine and 1.1 ng/g for simazine were obtained with recoveries of 84-95% for environmental samples. On the basis of practical application to five soil and five crop samples, the LC-FL method was compared with the conventional GC-MS method.
authors.affiliation: Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China. gongaij@mail.rcees.ac.cn
authors.forename: A
authors.initials: A
authors.lastname: Gong
authors.forename: C
authors.initials: C
authors.lastname: Ye
mesh_terms: D001280:Atrazine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D006540:Herbicides / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012839:Simazine / Q000032:analysis*; D013050:Spectrometry, Fluorescence
chemical_list: D004785:Environmental Pollutants; D006540:Herbicides; D001280:Atrazine; D012839:Simazine