2-Methoxyestradiol, an endogenous metabolite of 17beta-estradiol, inhibits adipocyte proliferation.
The effects of 2-methoxyestradiol (2ME), a naturally occurring mammalian metabolite of 17beta-estradiol, on adipocyte growth has been investigated in mouse brown adipose tissue precursor cells developed in primary culture. 2ME inhibits brown adipocyte proliferation in a dose-response manner (IC50 = 1.7 x 10(-6) M for DNA synthesis), with much higher potency than its hormone precursor 17beta-estradiol, and cells acquire the typical differentiated morphology--more round with a higher content of triglycerides. 2ME causes similar effects in the immortal brown adipocyte tumor-derived hibernoma cell line HIB 1B and the immortal 3T3-F442A white adipocyte line. These findings suggest a possible role for 2ME in adipocyte proliferation, and probably in the differentiation process, entering the cells in the adipogenic program.
Picó
C
C
Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Puigserver
P
P
Oliver
P
P
Palou
A
A
D000077584:2-Methoxyestradiol; D017667:Adipocytes / Q000187:drug effects* / Q000648:ultrastructure; D002001:Adipose Tissue, Brown / Q000166:cytology; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004958:Estradiol / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D014407:Tumor Cells, Cultured
D004958:Estradiol; D000077584:2-Methoxyestradiol