title: Simple and sensitive method for determination of metronidazole in human serum by high-performance liquid chromatography.
abstract: A simple and sensitive HPLC method for determination of metronidazole in human plasma has been developed. A step of freezing the protein precipitate allowed an efficient separation of aqueous and organic phases minimizing the noise level and improved therefore the limit of quantitation (10 ng ml(-1) using 1 ml of plasma sample). The separation of compounds was performed on a RP 18 column with acetonitrile-aqueous 0.01 M phosphate solution (15:85, v/v) as mobile phase. Detection was performed by UV absorbance at 318 nm. Metronidazole was well resolved from the plasma constituents and internal standard. An excellent linearity was observed between peak-height ratios plasma concentrations over a concentration range of 0.01 to 10 microg ml(-1). Within-day and between-day precision (expressed by relative standard deviation) and accuracy (mean error in per cent) did not exceed 4% between 1 and 10 microg ml(-1) and 8.3 and 7.2% respectively for the limit of quantitation. The method is suitable for bioavailability and pharmacokinetic studies in humans.
authors.affiliation: Groupe de Recherche en Biodynamique du Médicament, Laboratoire de Chimie Analytique et de Spectrométrie de Masse, Faculté de Pharmacie, Clermont-Ferrand, France.
authors.forename: M J
authors.initials: MJ
authors.lastname: Galmier
authors.forename: A M
authors.initials: AM
authors.lastname: Frasey
authors.forename: M
authors.initials: M
authors.lastname: Bastide
authors.forename: E
authors.initials: E
authors.lastname: Beyssac
authors.forename: J
authors.initials: J
authors.lastname: Petit
authors.forename: J M
authors.initials: JM
authors.lastname: Aiache
authors.forename: C
authors.initials: C
authors.lastname: Lartigue-Mattei
mesh_terms: D000994:Antitrichomonal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D008795:Metronidazole / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000994:Antitrichomonal Agents; D008795:Metronidazole