ACTH regulates LDL receptor and CLA-1 mRNA in the rat adrenal cortex.
Rat adrenocortical cells utilize both low density lipoprotein (LDL) and high density lipoprotein (HDL) cholesterol for steroid hormone production. In addition to exogenous lipoprotein-derived cholesterol, cells produce cholesterol de novo. Adrenocorticotropin (ACTH) increases both steroid hormone secretion and uptake of LDL and HDL. We studied the expression of LDL receptor mRNA and CLA-1 (a putative HDL receptor) mRNA in cultured rat adrenocortical cells. ACTH increased the amounts of LDL receptor mRNA during 2 to 48 h of stimulation, the highest levels being detected after 2-4 h. Similar results were obtained with cyclic AMP (cAMP) derivatives, 8-bromo cAMP (8-Br cAMP) or dibutyryl cAMP. ACTH increased CLA-1 mRNA during 2 to 24 h of stimulation, the highest levels being detected after 4 h. In conclusion, ACTH up regulates both LDL and HDL receptor mRNA in rat adrenocortical cells.
Heikkilä
P
P
Haartman Institute, Department of Pathology, University of Helsinki, Finland.
Arola
J
J
Liu
J
J
Kahri
A I
AI
D015124:8-Bromo Cyclic Adenosine Monophosphate / Q000494:pharmacology; D000302:Adrenal Cortex / Q000166:cytology / Q000196:embryology / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D018955:CD36 Antigens / Q000235:genetics*; D002478:Cells, Cultured; D008076:Cholesterol, HDL / Q000378:metabolism; D008078:Cholesterol, LDL / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats / Q000196:embryology; D011971:Receptors, Immunologic; D011973:Receptors, LDL / Q000235:genetics*; D018110:Receptors, Lipoprotein / Q000235:genetics*; D051116:Receptors, Scavenger; D051122:Scavenger Receptors, Class B; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology
D018955:CD36 Antigens; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D012333:RNA, Messenger; D011971:Receptors, Immunologic; D011973:Receptors, LDL; D018110:Receptors, Lipoprotein; D051116:Receptors, Scavenger; C495125:Scarb1 protein, rat; D051122:Scavenger Receptors, Class B; D015124:8-Bromo Cyclic Adenosine Monophosphate; D003994:Bucladesine; D000324:Adrenocorticotropic Hormone