Complementation of the growth defect of an rnpA49 mutant of Escherichia coli by overexpression of arginine tRNA(CCG).
We previously found that overexpression of arginine tRNA(CCG) from Brevibacterium albidum complements the rnpA49 mutation, which is responsible for the thermosensitivity of Escherichia coli RNase P function. In this present work, we show that the E. coli homologue tRNA also complements the same mutation, but other tRNAs do not. These results suggest that the rnpA49 mutation causes a major cellular defect in an RNase P reaction to generate the mature arginine tRNA(CCG).
Kim
M S
MS
Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon, Korea.
Park
B H
BH
Kim
S
S
Lee
Y J
YJ
Chung
J H
JH
Lee
Y
Y
D001483:Base Sequence; D001951:Brevibacterium / Q000201:enzymology / Q000235:genetics*; D017931:DNA Primers; D004722:Endoribonucleases / Q000737:chemistry / Q000235:genetics*; D004795:Enzyme Stability; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D029968:Escherichia coli Proteins; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D009690:Nucleic Acid Conformation; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000737:chemistry / Q000235:genetics*; D012347:RNA, Transfer, Arg / Q000737:chemistry / Q000235:genetics*; D012361:RNA, Transfer, Pro / Q000737:chemistry / Q000235:genetics; D043262:Ribonuclease P; D013816:Thermodynamics
D017931:DNA Primers; D029968:Escherichia coli Proteins; D016337:RNA, Catalytic; D012347:RNA, Transfer, Arg; D012361:RNA, Transfer, Pro; D004722:Endoribonucleases; D043262:Ribonuclease P; C470865:ribonuclease P, E coli