Protein-DNA complexes in mycobacteriophage L5 integrative recombination.
The temperate mycobacteriophage L5 integrates site specifically into the genomes of Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium bovis bacillus Calmette-Guérin. This integrative recombination event occurs between the phage L5 attP site and the mycobacterial attB site and requires the phage-encoded integrase and mycobacterial-encoded integration host factor mIHF. Here we show that attP, Int-L5, and mIHF assemble into a recombinationally active complex, the intasome, which is capable of attB capture and formation of products. The arm-type integrase binding sites within attP play specialized roles in the formation of specific protein-DNA architectures; the intasome is constructed by the formation of intramolecular integrase bridges between one pair of sites, P4-P5, and the attP core, while an additional pair of sites, P1-P2, is required for interaction with attB.
Peña
C E
CE
Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Kahlenberg
J M
JM
Hatfull
G F
GF
D001483:Base Sequence; D001665:Binding Sites; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000378:metabolism; D008242:Lysogeny; D009160:Mycobacteriophages / Q000235:genetics* / Q000378:metabolism*; D009163:Mycobacterium bovis / Q000235:genetics / Q000821:virology*; D020102:Mycobacterium smegmatis / Q000235:genetics / Q000821:virology*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000821:virology*; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D016662:Virus Integration
D004269:DNA, Bacterial; D014764:Viral Proteins; D019426:Integrases