title: Mechanisms for induction of acquired host immunity by neutrophil peptide defensins.
abstract: Human neutrophil peptide (HNP) defensins were studied to determine their potential effects on adaptive mucosal immunity. Intranasal delivery of HNPs plus ovalbumin (OVA) enhanced OVA-specific serum IgG antibody (Ab) responses. However, OVA-specific IgA Abs were not induced in mucosal secretions or in serum. CD4(+) T cells of intranasally immunized mice displayed higher OVA-specific proliferative responses and elevated production of interferon gamma, interleukin (IL) 5, IL-6, and IL-10 when compared with control groups receiving OVA alone. In vitro, HNPs also enhanced both proliferative responses and T helper (Th) cytokine secretion profiles of CD3epsilon-stimulated spleen- and Peyer's patch-derived naive CD4(+) T cells. HNPs modulated the expression of costimulatory molecules by lipopolysaccharide- or CD3epsilon-stimulated splenic and Peyer's patch B or T cell populations, respectively. These studies show that defensins enhance systemic IgG, but not IgA, Ab responses through help provided by CD4(+) Th1- and Th2-type cytokines and foster B and T cell interactions to link innate immunity with the adaptive immune system.
authors.affiliation: Department of Microbiology, University of Alabama, 273A BBRB, 845 19th Street South, Birmingham, AL 35294-2170, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Lillard
authors.forename: P N
authors.initials: PN
authors.lastname: Boyaka
authors.forename: O
authors.initials: O
authors.lastname: Chertov
authors.forename: J J
authors.initials: JJ
authors.lastname: Oppenheim
authors.forename: J R
authors.initials: JR
authors.lastname: McGhee
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D017252:CD3 Complex; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D023082:Defensins; D005260:Female; D006801:Humans; D007109:Immunity; D007074:Immunoglobulin G / Q000097:blood; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D009297:Nasal Mucosa / Q000276:immunology; D009504:Neutrophils / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D011506:Proteins / Q000276:immunology* / Q000494:pharmacology; D011948:Receptors, Antigen, T-Cell / Q000276:immunology; D013154:Spleen / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D017252:CD3 Complex; C115335:CD3E protein, human; D016207:Cytokines; D023082:Defensins; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011506:Proteins; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin