Pulsed-field gel electrophoresis is more efficient than ribotyping and random amplified polymorphic DNA analysis in discrimination of Pasteurella haemolytica strains.
One hundred thirty-three strains of Pasteurella haemolytica of both biotypes (90 and 43 strains of biotypes A and T, respectively) and almost all the serotypes were subjected to ribotyping, random amplified polymorphic DNA (RAPD) analysis, and pulsed-field gel electrophoresis (PFGE) analysis for epidemiological purposes. A total of 15 patterns recorded as ribotypes HA to HO were found for the P. haemolytica biotype A strains, with ribotypes HA, HC, and HD being encountered most often (66 strains [74%]); and 20 ribotypes, designated HA' to HT', that were clearly distinct from those observed for biotype A strains were observed for strains of biotype T. RAPD analysis generated a total of 44 (designated Rp1 to Rp44) and 15 (designated Rp1' to Rp 15') unique RAPD patterns for biogroup A and biogroup T, respectively. Analysis of the data indicated that a given combined ribotype-RAPD pattern could be observed for biotype A strains of different serotypes, whatever the zoological or geographic origin, whereas this was not the case for biotype T strains. PFGE appeared to be more efficient in strain discrimination since selected strains from various zoological or geographical origins harboring the same ribotype-RAPD group were further separated into unique entities.
Kodjo
A
A
Ecole Nationale Vétérinaire de Lyon, F-69280 Marcy l'Etoile, France. a.kodjo@vet-lyon.fr
Villard
L
L
Bizet
C
C
Martel
J L
JL
Sanchis
R
R
Borges
E
E
Gauthier
D
D
Maurin
F
F
Richard
Y
Y
D000818:Animals; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000737:chemistry; D004275:DNA, Ribosomal / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D020459:Genes, rRNA; D016978:Mannheimia haemolytica / Q000145:classification* / Q000235:genetics*; D010326:Pasteurella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D012335:RNA, Ribosomal / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D015183:Restriction Mapping
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012335:RNA, Ribosomal