title: Tyrosine phosphorylation and translocation of LAT in platelets.
abstract: Platelet aggregation is accompanied by the tyrosine phosphorylation of several proteins including syk. However, some of these proteins are not identified. Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation. In this study, we demonstrated that LAT is present in platelets and is tyrosine phosphorylated in response to ADP- and thrombin-stimulated aggregation. Moreover, LAT, like syk and beta3, translocates to the cytoskeleton during the late stage of thrombin-stimulated irreversible aggregation and not during ADP-stimulated reversible aggregation.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill 27599-7525, USA. sarkarf@med.unc.edu
authors.forename: S
authors.initials: S
authors.lastname: Sarkar
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000244:Adenosine Diphosphate / Q000494:pharmacology; D001692:Biological Transport; D001792:Blood Platelets / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism*; D004792:Enzyme Precursors / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D019169:Jurkat Cells; D008565:Membrane Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D010974:Platelet Aggregation / Q000187:drug effects; D011505:Protein-Tyrosine Kinases / Q000378:metabolism; D015531:Radioimmunoprecipitation Assay; D000072377:Syk Kinase; D013917:Thrombin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D004792:Enzyme Precursors; D047908:Intracellular Signaling Peptides and Proteins; C110810:LAT protein, human; D008565:Membrane Proteins; D010750:Phosphoproteins; D014443:Tyrosine; D000244:Adenosine Diphosphate; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase; D013917:Thrombin