title: Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory.
abstract: The mutable flaked (or af) lines of the common morning glory bear white flowers with colored flakes and sectors. The af allele shows incomplete dominance. Plants in the heterozygous state A/af bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable af allele is caused by insertion of a new transposable element, Tip100, into the CHS-D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100-1. The structure of Tip100-1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100. Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS-D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.
authors.affiliation: National Institute for Basic Biology, Aichi, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Habu
authors.forename: Y
authors.initials: Y
authors.lastname: Hisatomi
authors.forename: S
authors.initials: S
authors.lastname: Iida
mesh_terms: D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D008969:Molecular Sequence Data; D009154:Mutation; D010858:Pigmentation / Q000235:genetics; D010944:Plants / Q000235:genetics*
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements