title: Lymphokine-activated killer cell susceptibility and multidrug resistance in small cell lung carcinoma.
abstract: Intrinsic or acquired resistance to anticancer drugs necessitated the search for different treatment modalities. The sensitivity of tumor cells to lysis by natural killer (NK) and lymphokine-activated killer (LAK) cells was studied in multidrug resistant (MDR) small cell lung carcinoma (SCLC) by 51Chromium (51Cr) release and conjugate formation assays. The following observations were made: P-glycoprotein positive (P-gp+) MDR SCLC cell line variants were lysed by human LAK cells to a greater extent than were their drug sensitive counterparts. In contrast, P-gp, multidrug resistance protein positive (MRP+) variants of the same line did not exhibit an increased susceptibility to LAK cells. Differential LAK susceptibility is not due to a generalized increase in target fragility to cellular immunity, because NK sensitivity was not increased. Moreover, the P-gp+ MDR SCLC cells showed a higher frequency of binding to LAK cells than did the drug-sensitive parental line. These observations may lead to new insights on combining chemotherapy with immunotherapy.
authors.affiliation: Department of Immunology, Queen's University Kingston, Canada. Savasb@med,akdeniz.edu.tr
authors.forename: B
authors.initials: B
authors.lastname: Savas
authors.forename: P E
authors.initials: PE
authors.lastname: Kerr
authors.forename: H
authors.initials: H
authors.lastname: Ustun
authors.forename: S P
authors.initials: SP
authors.lastname: Cole
authors.forename: H F
authors.initials: HF
authors.lastname: Pross
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D018288:Carcinoma, Small Cell / Q000235:genetics / Q000276:immunology*; D002470:Cell Survival; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D018432:Drug Resistance, Multiple; D006801:Humans; D020014:K562 Cells; D015979:Killer Cells, Lymphokine-Activated / Q000276:immunology*; D008175:Lung Neoplasms / Q000235:genetics / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D012016:Reference Values; D014407:Tumor Cells, Cultured
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1