title: [Comparison of the microdilution method (PASCO), the Etest and disc-diffusion in streptococcus pneumoniae antibiotic susceptibility testing].
abstract: MIC values and inhibition zone diameters for penicillin, cefotaxime, and erythromycin where determined in 75 consecutive Streptococcus pneumoniae blood isolates by microdilution (PASCO), disc-diffusion and Etest. Using the Etest, 40% and 29.3% of the isolates showed low- or high-level resistance to penicillin and erythromycin respectively, whereas 9.3% were intermediate to cefotaxime. No high-level resistance to cefotaxime was found. No errors were found with the comparison of penicillin and cefotaxime results by microdilution and Etest, with just 2 (2.7%) and 3 (4%) minor errors appearing respectively. On the other hand, when comparing penicillin susceptibility results by disc-diffusion (1 mg oxacillin disc) and Etest, 6 (8%) major errors and 18 (24%) minor errors were found.
authors.affiliation: Servicio de Microbiología, Hospital Ramón y Cajal, Carretera Colmenar km 9,1, 28034 Madrid.
authors.forename: A
authors.initials: A
authors.lastname: Oliver
authors.forename: D
authors.initials: D
authors.lastname: Tarragó
authors.forename: M
authors.initials: M
authors.lastname: Martínez-Ferrer
authors.forename: E
authors.initials: E
authors.lastname: Loza
authors.forename: R
authors.initials: R
authors.lastname: Cantón
authors.forename: F
authors.initials: F
authors.lastname: Baquero
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media; D004058:Diffusion; D007201:Indicator Dilution Techniques; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013296:Streptococcus pneumoniae / Q000187:drug effects*