Carbon catabolite repression of the Aspergillus nidulans xlnA gene.
Expression of the Aspergillus nidulans 22 kDa endoxylanase gene, xlnA, is controlled by at least three mechanisms: specific induction by xylan or xylose; carbon catabolite repression (CCR); and regulation by ambient pH. Deletion analysis of xlnA upstream sequences has identified two positively acting regions: one that mediates specific induction by xylose; and another that mediates the influence of ambient pH and contains two PacC consensus binding sites. The extreme derepressed mutation creAd30 results in considerable, although not total, loss of xlnA glucose repressibility, indicating a major role for CreA in its CCR. Three consensus CreA binding sites are present upstream of the structural gene. Point mutational analysis using reporter constructs has identified a single site, xlnA.C1, that is responsible for direct CreA repression in vivo. Using the creAd30 derepressed mutant background, our results indicate the existence of indirect repression by CreA.
Orejas
M
M
Departamento de Biotecnología, Consejo Superior de Investigaciones Científicas, Valencia, Spain.
MacCabe
A P
AP
Pérez González
J A
JA
Kumar
S
S
Ramón
D
D
D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D001665:Binding Sites; D002244:Carbon / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D016297:Mutagenesis, Site-Directed; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D014995:Xylosidases / Q000235:genetics*