Tryptophan pyrolysis products, Trp-P-1 and Trp-P-2 induce apoptosis in primary cultured rat hepatocytes.
The cytotoxicity of heterocyclic amines, dietary carcinogens derived from cooked foods, to primary cultured rat hepatocytes was studied. A tryptophan pyrolysis product, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) was the most cytotoxic of 11 compounds tested. Trp-P-1 was found to induce apoptosis as measured by morphological changes in nuclear chromatin and internucleosomal DNA fragmentation. 3-Amino-1-methyl-5H-pyrido[4,3-b] indole (Trp-P-2) showed a moderate apoptotic effect, and other compounds had a similar but weaker effect.
Ashida
H
H
Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, Japan. ashida@kobe-u.ac.jp
Shiotani
B
B
Adachi
H
H
Hashimoto
T
T
Kanazawa
K
K
Danno
G
G
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002243:Carbolines / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D053938:DNA Fragmentation; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008297:Male; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014364:Tryptophan / Q000378:metabolism*