title: Three-dimensional partial zona dissection for preimplantation genetic diagnosis and assisted hatching.
abstract: OBJECTIVE
  To develop a new approach to partial zona dissection of oocytes and embryos for facilitating preimplantation genetic sampling and assisted hatching.
  
  
  METHODS
  Controlled clinical study.
  
  
  METHODS
  Preimplantation genetic diagnosis and IVF program, Reproductive Genetics Institute/IVF Illinois, Chicago, Illinois.
  
  
  METHODS
  Three hundred forty patients undergoing IVF in whom preimplantation genetic diagnosis or assisted hatching was required.
  
  
  METHODS
  Three-dimensional partial zona dissection and conventional partial zona dissection were performed, with the use of a simple microneedle, on embryos before preimplantation genetic sampling or on day 3 of embryo development before ET.
  
  
  METHODS
  Pregnancy rate, implantation rate, and ease of preimplantation genetic sampling.
  
  
  RESULTS
  A pregnancy rate of 42% and an embryo implantation rate of 17.6% were obtained in the group in which three-dimensional partial zona dissection was performed, compared with a pregnancy rate of 33% and an implantation rate of 14.7% in the control group, which underwent conventional partial zona dissection. Preimplantation genetic sampling can be performed without distortion of the blastomere configuration in the embryo.
  
  
  CONCLUSIONS
  Three-dimensional partial zona dissection is a safe and simple mechanical means of creating an opening sufficient for the atraumatic removal of material from oocytes and embryos for preimplantation genetic diagnosis and assisted hatching.
authors.affiliation: Reproductive Genetics Institute, Illinois Masonic Medical Center, Chicago 60657, USA. rgi@flash.net
authors.forename: J
authors.initials: J
authors.lastname: Cieslak
authors.forename: V
authors.initials: V
authors.lastname: Ivakhnenko
authors.forename: G
authors.initials: G
authors.lastname: Wolf
authors.forename: S
authors.initials: S
authors.lastname: Sheleg
authors.forename: Y
authors.initials: Y
authors.lastname: Verlinsky
mesh_terms: D004210:Dissection; D010064:Embryo Implantation; D005260:Female; D005307:Fertilization in Vitro; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*; D015044:Zona Pellucida