A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats.
We describe a reliable and inexpensive method for placing injections of anatomical tracers into the brain of lower mammals. The pressure microinjecting system we developed is specifically designed to deliver very small amount of substances. The injecting portion of the system is relatively easy to assemble and can be repeatedly used for multiple experimental sessions. The system has been validated with experiments of multiple fluorescent retrograde tracing. In these experiments the populations of thalamo-cortical neurons were consistently labeled by the tracers injected bilaterally and symmetrically in the cortex of foetal and neonatal rats.
Sbriccoli
A
A
Department of Neurological and Psychiatric Sciences, University of Florence, Italy.
Carretta
D
D
Santarelli
M
M
Pinto
F
F
Granato
A
A
Minciacchi
D
D
D000578:Amidines / Q000008:administration & dosage* / Q000493:pharmacokinetics; D000818:Animals; D000831:Animals, Newborn; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000196:embryology* / Q000254:growth & development*; D004396:Coloring Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D005260:Female; D008845:Microinjections / Q000295:instrumentation*; D009434:Neural Pathways / Q000196:embryology* / Q000254:growth & development*; D011247:Pregnancy; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D013788:Thalamus / Q000196:embryology* / Q000254:growth & development*