title: Histone modification governs the cell cycle regulation of a replication-independent chromatin assembly pathway in Saccharomyces cerevisiae.
abstract: We describe a replication-independent, cell cycle-regulated chromatin assembly pathway in budding yeast. The activity of this pathway is low in S phase extracts but is very high in G2, M, and G1 cell extracts, with peak activity in late M/early G1. The cell cycle regulation of this pathway requires a specific pattern of posttranslational modification of histones H3 and/or H4, which is distinct for H3/H4 present in S phase versus M and G1 phase cell extracts. Histone H3/H4 modification is therefore important for the reciprocal control of replication-dependent and -independent chromatin assembly pathways during the cell cycle.
authors.affiliation: Department of Biochemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2H7.
authors.forename: B A
authors.initials: BA
authors.lastname: Altheim
authors.forename: M C
authors.initials: MC
authors.lastname: Schultz
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002843:Chromatin / Q000502:physiology*; D004261:DNA Replication; D005434:Flow Cytometry; D016193:G1 Phase; D016195:G2 Phase; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D008938:Mitosis; D011499:Protein Processing, Post-Translational; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D013997:Time Factors