title: Purification, characterization, and sequence determination of phospholipase D secreted by Streptoverticillium cinnamoneum.
abstract: Phospholipase D (PLD), secreted into the culture medium of an actinomycete, Streptoverticillium cinnamoneum, has been purified to homogeneity and characterized. The Stv. cinnamoneum PLD efficiently catalyzes both the hydrolysis and transphosphatidylation of various phospholipids, including phosphatidylethanolamine (PE), phosphatidylcholine (PC), and phosphatidylserine (PS). However, the substrate specificity differs between the two reactions; PE serves as the most preferred substrate for the hydrolysis, but PC and PS are better substrates than PE for the transphosphatidylation. In addition, the transphosphatidylation but not the hydrolysis of PE and PC is markedly activated on the addition of metal ions, especially Al3+. Nucleotide and amino acid sequence determination of the Stv. cinnamoneum PLD revealed the presence of common structural motifs identified in all PLD sequences from various species.
authors.affiliation: Graduate School of Science and Technology, Nagoya University, Nagoya, 461-8601, Japan.
authors.forename: C
authors.initials: C
authors.lastname: Ogino
authors.forename: Y
authors.initials: Y
authors.lastname: Negi
authors.forename: T
authors.initials: T
authors.lastname: Matsumiya
authors.forename: K
authors.initials: K
authors.lastname: Nakaoka
authors.forename: A
authors.initials: A
authors.lastname: Kondo
authors.forename: S
authors.initials: S
authors.lastname: Kuroda
authors.forename: S
authors.initials: S
authors.lastname: Tokuyama
authors.forename: U
authors.initials: U
authors.lastname: Kikkawa
authors.forename: T
authors.initials: T
authors.lastname: Yamane
authors.forename: H
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D000535:Aluminum / Q000378:metabolism*; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000032:analysis; D006868:Hydrolysis; D008361:Mannosidases / Q000302:isolation & purification* / Q000378:metabolism; D008670:Metals / Q000378:metabolism; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D013306:Streptomycetaceae / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity