Concurrent increase of oxidative DNA damage and lipid peroxidation together with mitochondrial DNA mutation in human lung tissues during aging--smoking enhances oxidative stress on the aged tissues.
Although mutation of mitochondrial DNA (mtDNA) in human tissues has been established to associate with intrinsic aging, the impact of environmental factors on the formation and accumulation of mtDNA mutations and oxidative DNA damage in human tissues is poorly understood. We have investigated the levels of mtDNA with the 4977-bp deletion and A3243G point mutation, oxidative DNA damage (indicated by the formation of 8-hydroxy-2'-deoxyguanosine, 8-OH-dG), and lipid peroxides in lung tissues from smokers and nonsmokers of subjects of different ages. The results showed concurrent age-dependent increase of the 4977-bp deleted mtDNA (P < 0.001), 8-OH-dG (P < 0.05), and lipid peroxides (P < 0.05) in the human lung. In the group of subjects above 60 years old, smokers had more extensive DNA damage and lipid peroxidation than did the nonsmokers. However, the levels of mtDNA with the 4977-bp deletion and A3243G point mutation in the lung of smokers were not significantly different from those of the age-matched nonsmokers. Taken together, these results suggest that accumulation of mtDNA with the 4977-bp deletion together with oxidative DNA damage and lipid peroxides is associated with aging and that smoking enhances oxidative damage in human lung tissues.
Lee
H C
HC
Department of Biochemistry and Center for Cellular and Molecular Biology, National Yang-Ming University, Taipei, 112, Taiwan.
Lim
M L
ML
Lu
C Y
CY
Liu
V W
VW
Fahn
H J
HJ
Zhang
C
C
Nagley
P
P
Wei
Y H
YH
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000293:Adolescent; D000328:Adult; D000368:Aged; D000375:Aging; D002648:Child; D002675:Child, Preschool; D004249:DNA Damage / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D006801:Humans; D007223:Infant; D015227:Lipid Peroxidation; D008054:Lipid Peroxides / Q000032:analysis; D008168:Lung / Q000737:chemistry / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D016555:Matched-Pair Analysis; D008875:Middle Aged; D009154:Mutation; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D017354:Point Mutation / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D012907:Smoking / Q000503:physiopathology*