In vivo administration of granulocyte colony-stimulating factor increases the surface expression of sialyl-Lewis(x) on neutrophils in healthy volunteers.
We examined the in vivo effect of granulocyte colony-stimulating factor (G-CSF) on the surface expression of putative counterligands for endothelial selectins on neutrophils in healthy volunteers. G-CSF (50 microg/m2/day) was administered subcutaneously to 5 healthy volunteers for 4 days. The expression of surface antigens on neutrophils was determined by flow cytometry and monoclonal antibodies. G-CSF administration increased the number of leukocytes, mainly of neutrophils, which was associated with an increase in the expression of the high-affinity Fc receptor for IgG (FcRI, CD64) and CD14 on neutrophils. G-CSF administration decreased the surface expression of L-selectin on neutrophils, whereas it increased the expression of sialyl-Lewisx but not Lewisx on neutrophils. These findings suggest that G-CSF participates in the neutrophil-endothelial cell interactions in vivo by modulating the expression of adhesion molecules and ligands for endothelial selectins on neutrophils.
Ohsaka
A
A
Department of Internal Medicine, Division of Hematology, Hitachi General Hospital, Ibaraki, Japan. oosaka@cm.nichibyo.hitachi.co.jp
Saionji
K
K
D005732:Gangliosides / Q000096:biosynthesis*; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage* / Q000494:pharmacology*; D006801:Humans; D007279:Injections, Subcutaneous; D019041:L-Selectin / Q000096:biosynthesis; D007958:Leukocyte Count; D007983:Lewis Blood Group Antigens / Q000096:biosynthesis*; D018950:Lipopolysaccharide Receptors / Q000096:biosynthesis; D008565:Membrane Proteins / Q000096:biosynthesis; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D017452:Receptors, IgG / Q000096:biosynthesis; D000080506:Sialyl Lewis X Antigen