Offset profiles of selective pulses in isotopically labeled macromolecules.
The experimental verification of offset profiles and calibration of selective pulses in NMR is usually carried out with doped water samples but not under conditions typical of macromolecules with short T2, long T1, and possibly homo- and heteronuclear couplings. A new method for selective excitation in isotopically labeled macromolecules is shown to be particularly suited to this purpose. This is illustrated for a backbone amide resonance in a sample of 15N-labeled human ubiquitin.
Eykyn
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Section de Chimie, Université de Lausanne, BCH, Lausanne, 1015, Switzerland.
Ghose
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Bodenhausen
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D000577:Amides; D002247:Carbon Isotopes; D006801:Humans; D046911:Macromolecular Substances; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D012680:Sensitivity and Specificity; D013113:Spin Labels; D014452:Ubiquitins / Q000138:chemical synthesis / Q000737:chemistry*