Cleavage of L-leucine-containing dipeptides by Clostridium butyricum.
The ability of Clostridium butyricum cultures to hydrolyze three L-leucine-containing dipeptides (Leu-Leu, Leu-Gly and Gly-Leu) in a synthetic minimal medium is demonstrated by using gas chromatography coupled with mass spectrometry. The 13C nuclear magnetic resonance and a labeled dipeptide L-[1-13C]Leu-Gly were used to confirm this activity.
Khelifa
N
N
Laboratoire de Microbiologie, UMA, Faculté des Sciences Pharmaceutiques et Biologiques de Paris, Université René Descartes, Paris, France.
Brik
M
M
Tessedre
A C
AC
De Rocquigny
H
H
Roques
B P
BP
Courtieu
J
J
Rimbault
A
A
D002247:Carbon Isotopes; D002849:Chromatography, Gas / Q000379:methods; D003013:Clostridium / Q000378:metabolism*; D004151:Dipeptides / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry