[Ultrastructural and immunocytochemical study of Listeria monocytogenes with varying levels of pathogenicity factor production].
Wild and mutant strains of Listeria monocytogenes are examined by electron and immunoelectron microscopy. The mutant strain was characterized as a strain with a high level of production of pathogenic factors. No essential morphological criteria permitting the differentiation between wild and mutant Listeria strains were detected. Addition of activated charcoal to nutrient medium resulted in similar morphological changes in both strains. Enlargement of cells, a thicker cell wall, and changes in the cytoplasm structure are objective morphological signs of functional activity of bacteria with a higher level of pathogenic factor production. Indirect immunocytochemical method demonstrated the localization of specific phosphatidyl inosityl phospholipase C.
Didenko
L V
LV
Ermolaeva
S A
SA
Konstantinova
N D
ND
Varfolomeeva
N A
NA
Tartakovskiĭ
I S
IS
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003470:Culture Media; D007150:Immunohistochemistry; D008089:Listeria monocytogenes / Q000378:metabolism / Q000472:pathogenicity* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods; D014774:Virulence