title: Core promoter elements can regulate transcription on a separate chromosome in trans.
abstract: Transvection can cause the expression of a gene to be sensitive to the proximity of a homolog. It can account for many cases of intragenic complementation at the Drosophila yellow gene, where one mode of transvection involves the action of enhancers in trans on a promoter present on a separate chromosome. Our goal was to identify cis-acting elements that regulate the trans action of enhancers. Using gene replacement, we altered two core promoter elements at yellow and tested the resulting alleles for their ability to support transvection. We found that the TATA box and initiator element can regulate transvection.
authors.affiliation: Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA.
authors.forename: J R
authors.initials: JR
authors.lastname: Morris
authors.forename: P K
authors.initials: PK
authors.lastname: Geyer
authors.forename: C T
authors.initials: CT
authors.lastname: Wu
mesh_terms: D029721:Drosophila Proteins; D005786:Gene Expression Regulation; D019476:Insect Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D016385:TATA Box; D014158:Transcription, Genetic