Comparison of four monoclonal antibodies reacting with gastric gland mucous cell-derived mucins of rat and frog.
Features of four monoclonal antibodies (MAbs), PGM36, PGM37, PGM38 and HIK1083, reacting with the mucin derived from rat gastric gland mucous cells were compared. By applying enzyme linked immunosorbent assay, all of these MAbs reacted not only with the mucins purified from both rat and frog stomach, but also with the oligosaccharides obtained from the antigenic mucins by alkaline borohydride treatment. These MAbs could be characterized as distinct MAbs due to the immunohistochemical observation of rat cecal mucosa and the reactivity to paranitrophenyl derivatives of monosaccharides. These MAbs might be useful tools to compare the gastric gland-type mucins in different species of vertebrates and to investigate the heterogeneity of the carbohydrate structure of the mucin molecules of various origins.
Kurihara
M
M
Isehara Research Laboratory, Kanto Chemical Co. Inc., Isehara, Japan.
Ishihara
K
K
Ota
H
H
Katsuyama
T
T
Nakano
T
T
Naito
M
M
Hotta
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry; D005260:Female; D005752:Gastric Mucins / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D005753:Gastric Mucosa / Q000737:chemistry* / Q000276:immunology*; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000276:immunology; D011892:Rana catesbeiana; D051381:Rats; D017207:Rats, Sprague-Dawley; D013045:Species Specificity; D013552:Swine