Cell position-dependent reciprocal feedback regulation of type I collagen gene expression in cultured human skin fibroblasts.
In order to investigate possible cell positional effects on the gene expression of human dermal fibroblasts, the authors cultured the cells on non-coated polystyrene culture dishes, type I collagen-coated dishes, or collagen gels formed by type I collagen, or suspended them in type I collagen gels and measured collagen synthesis by the cells. The production rate of type I collagen was similar whether cells were cultured on non-coated polystyrene or on type I collagen-coated dishes, but it was suppressed significantly when the cells were placed within the collagen gel matrix. Time-dependent expression of genes for alpha 1 (I) and alpha 2 (I) collagen chains was measured by Northern blot analysis. A significant increase in mRNA levels for these chains was observed when the cells were cultured for three days on type I collagen-coated dishes or on collagen gels. On the other hand, a significant decrease in the mRNA levels was observed after 2 days and later, when the cells were cultured within type I collagen gel matrix. These results indicate that human dermal fibroblasts recognize their position on or in type I collagen (extracellular matrix) and respond by changing their expression patterns of type I collagen chain genes. The results of the kinetics of gene expression also suggest that upregulation and downregulation of type I collagen genes are controlled by different mechanisms.
Hata
R
R
Department of Molecular Pathogenesis, Tokyo Medical and Dental University, Japan. r.hata.tis@mri1.mri.tmd.ac.jp
Akai
J
J
Kimura
A
A
D015152:Blotting, Northern; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology; D005246:Feedback; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D011137:Polystyrenes; D011392:Proline / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000378:metabolism*