title: Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication.
abstract: Sister chromatid cohesion is crucial for chromosome segregation during mitosis. Loss of cohesion very possibly triggers sister separation at the metaphase --> anaphase transition. This process depends on the destruction of anaphase inhibitory proteins like Pds1p (Cut2p), which is thought to liberate a sister-separating protein Esp1p (Cut1p). By looking for mutants that separate sister centromeres in the presence of Pds1p, this and a previous study have identified six proteins essential for establishing or maintaining sister chromatid cohesion. Four of these proteins, Scc1p, Scc3p, Smc1p, and Smc3p, are subunits of a 'Cohesin' complex that binds chromosomes from late G1 until the onset of anaphase. The fifth protein, Scc2p, is not a stoichiometric Cohesin subunit but it is required for Cohesin's association with chromosomes. The sixth protein, Eco1p(Ctf7p), is not a Cohesin subunit. It is necessary for the establishment of cohesion during DNA replication but not for its maintenance during G2 and M phases.
authors.affiliation: Research Institute of Molecular Pathology (IMP), A-1030 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Tóth
authors.forename: R
authors.initials: R
authors.lastname: Ciosk
authors.forename: F
authors.initials: F
authors.lastname: Uhlmann
authors.forename: M
authors.initials: M
authors.lastname: Galova
authors.forename: A
authors.initials: A
authors.lastname: Schleiffer
authors.forename: K
authors.initials: K
authors.lastname: Nasmyth
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D017124:Conserved Sequence; D004261:DNA Replication; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D005800:Genes, Fungal; D051379:Mice; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D010750:Phosphoproteins; D016196:S Phase; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins; D017386:Sequence Homology, Amino Acid; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1