Digoxigenin-labeled probe for rapid identification of nisinogenic Lactococcus lactis strains.
From the nisZ gene sequence, a non-radioactive digoxigenin-labeled DNA probe, was tested for detection of nisin-producing strains using polymerase chain reaction amplification. The digoxigenin-labeled DNA probe clearly discriminated between nisin-producing and non-producing strains with a high degree of sensitivity and specificity. By agarose gel electrophoresis, 1.4 ng of nisin DNA was detected using the digoxigenin-labeled DNA probe compared with 11 ng using direct polymerase chain reaction amplification. A colony hybridization method using digoxigenin-labeled DNA to selectively detect nisinogenic bacteria showed that the nis-probe was specific and did not react with any other non-bacteriocinogenic and non-nisinogenic strains.
Meghrous
J
J
Centre de Recherche en Sciences et Technologies du Lait (STELA), Université Laval, Sainte-Foy, Que., Canada.
Fliss
I
I
Bouksaim
M
M
Lacroix
C
C
D015342:DNA Probes; D004076:Digoxigenin; D005520:Food Preservatives / Q000737:chemistry; D005798:Genes, Bacterial / Q000235:genetics*; D013294:Lactococcus lactis / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D015336:Molecular Probe Techniques; D009561:Nisin / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D013045:Species Specificity