galectin-1 and galectin-3 expression in human bladder transitional-cell carcinomas.
Galectin-1 and galectin-3 are galactoside-binding proteins involved in different steps of tumor progression and potential targets for therapy. We have investigated the expression of these galectins in 38 human bladder transitional-cell carcinomas of different histological grade and clinical stage and in 5 normal urothelium samples. Galectin-1 mRNA levels were highly increased in most high-grade tumors compared with normal bladder or low-grade tumors. Western blot and immuno-histochemical analysis of normal and neoplastic tissues revealed a higher content of galectin-1 in tumors. Galectin-3 mRNA levels were also increased in most tumors compared with normal urothelium, but levels were comparable among tumors of different histological grade.
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Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Centro di Endocrinologia ed Oncologia Sperimentale G. Salvatore del Consiglio Nazionale delle Ricerche, Università degli Studi di Napoli Federico II, Naples, Italy.
Benvenuto
G
G
Salvatore
P
P
Pero
R
R
Salvatore
G
G
Mirone
V
V
Prezioso
D
D
Altieri
V
V
Bruni
C B
CB
Chiariotti
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D037483:Galectin 1; D037502:Galectin 3; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D014521:Urethra / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology