Detection of human papillomavirus types in cervical adenocarcinoma by the polymerase chain reaction.
OBJECTIVE
To determine the human papillomavirus (HPV) types in cervical adenocarcinoma of patients from Taiwan.


METHODS
DNA was extracted from fixed tissues and polymerase chain reaction was performed in conjunction with a unique probe, pRSA I, allowing simultaneous detection of HPV types 6, 11, 16, 18, 31 and 33 from amplified HPV DNAs after endonuclease, RsaI, digestion.


RESULTS
Of 69 tissues examined, 31.9% (22/69) were found to contain HPV DNA. Among 22 HPV-positive specimens, no HPV types 6, 11, 31 and 33 were detected. On the other hand, HPV 16 and HPV 18 were found in 11 (15.9%) and 10 (14.5%) of HPV-positive specimens, respectively. One specimen (1.5%) was found to contain both HPV 16 and 18 DNAs.


CONCLUSIONS
Our findings support that HPV 18, along with HPV 16, may play a certain role in the adenocarcinoma pathogenesis of the uterine cervix.
Lee
M F
MF
Department of Medical Research, Taichung Veterans General Hospital, Taiwan.
Chang
M C
MC
Wu
C H
CH
D000230:Adenocarcinoma / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D006801:Humans; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology*; D016133:Polymerase Chain Reaction; D013624:Taiwan / Q000453:epidemiology; D014412:Tumor Virus Infections / Q000453:epidemiology*; D002583:Uterine Cervical Neoplasms / Q000453:epidemiology / Q000821:virology*