Deoxyribonuclease I phenotyping from saliva stains.
Good typing results were obtained using a newly developed method for extraction and purification of deoxyribonuclease I (DNase I) from saliva stains. Previously, DNase I phenotyping from saliva stains has been unsuccessful because of low enzyme activity and heavy contamination. Salivary DNase I was extracted from stains using phosphate buffer containing Nonidet P-40. Extracts were purified using Phenyl Sepharose CL-4B gel. Electrophoresis was performed, and DNase I was successfully phenotyped. All of the DNase I phenotypes, which were obtained from saliva stains using this new method, were identical to the phenotypes determined from urine samples. Moreover, DNase I was correctly phenotyped from saliva stains that had been stored for over three months at room temperature or at 37 degrees C. These results suggest that DNase I polymorphisms provide valuable information for forensic characterization of saliva stains.
Iida
R
R
Department of Forensic Medicine, Fukui Medical University, Japan.
Sawazaki
K
K
Yasuda
T
T
Tsubota
E
E
Aoyama
M
M
Matsuki
T
T
Kishi
K
K
D004396:Coloring Agents; D003850:Deoxyribonuclease I / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010641:Phenotype; D012463:Saliva / Q000201:enzymology*