Parathyroids and ultimobranchial bodies in monotremes.
Only scant information is available in the scientific literature on the parathyroids and ultimobranchial bodies in the primitive mammals, the echidna (Tachyglossus aculeatus) and platypus (Ornithorhynchus anatinus). The major aim of this paper is to describe the morphology of the monotreme parathyroid gland and to compare it with parathyroids in mammals and reptiles. The gross anatomy and light microscopic structure of the ultimobranchial body, thymus, and thyroid are also given. Animals were dissected and routine light and electron microscopic techniques used to examine the microscopic morphology. The locations of parathyroid hormone, calcitonin and calcitonin gene-related peptide in tissue sections were identified by immunostaining. Monotremes have one pair of parathyroid glands located in the thorax and they are often associated with thymic tissue but never with the thyroid which is also present in the mediastinum. Ultimobranchial bodies are ventrolateral to the commencement of the trachea. Thymic lobules with Hassall's corpuscles are scattered in the fibrofatty tissue of the mediastinum and the ventral surface of the pericardium. Histologically, principal cells, water-clear cells, and non-secretory cells were identified in the parathyroid glands. Principal cells showed polarity and had microlamellar projections that formed intercellular canaliculi. Non-secretory cells had features similar to those of thymic epithelial reticular cells. Immunostaining of parathyroid hormone showed a diffuse distribution in parathyroid principal cells and none in ultimobranchial bodies. Identification of the ultimobranchial bodies was confirmed by immunostaining. The monotreme parathyroid gland, ultimobranchial bodies and thyroid show reptilian as well as mammalian features.
Haynes
J I
JI
Department of Anatomical Sciences, University of Adelaide, Australia. jhaynes@medicine.adelaide.edu.au
D000818:Animals; D002116:Calcitonin / Q000032:analysis; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis; D005260:Female; D008297:Male; D016253:Microscopy, Immunoelectron; D009007:Monotremata / Q000033:anatomy & histology*; D010280:Parathyroid Glands / Q000737:chemistry / Q000166:cytology*; D010281:Parathyroid Hormone / Q000032:analysis; D010987:Platypus / Q000033:anatomy & histology; D004442:Tachyglossidae / Q000033:anatomy & histology; D014460:Ultimobranchial Body / Q000737:chemistry / Q000166:cytology*