Clinical value of using an automated sperm morphology analyzer (IVOS).
OBJECTIVE
To determine the clinical value of automated normal sperm morphology outcomes.


METHODS
Prospective clinical study.


METHODS
Clinical and research assisted reproduction laboratory.


METHODS
Two hundred seven GIFT cycles.


METHODS
The wife was induced to superovulate, laparoscopically aspirated, and the gametes were transferred laparoscopically. The husband's sperm morphology was evaluated with use of a sperm morphology analyzer using the strict criteria classification system.


METHODS
Normal sperm morphology, IVF, and pregnancy outcomes.


RESULTS
The logistic regression model showed that normal sperm morphology was significantly associated with fertilization in vitro, as dependent (age) and independent variables. Analyzing the fertilization rates across the 5% normal sperm morphology cutoff point, a fertilization rate of 39.39% (< or = 5%) compared with 62.92% (>5%) was obtained. The logistic regression model showed that normal sperm morphology was also a significant predictor of pregnancy when allowing for the number of oocytes transferred and female age. Analyzing the pregnancy rates across the 5% normal sperm morphology cutoff point, pregnancy rates of 15.15% (< or = 5%) and 37.36% (>5%) were obtained.


CONCLUSIONS
Normal sperm morphology as evaluated by the automated semen analyzer (IVOS) was shown to adhere to the same fertility cutoff point (5%), as determined by the manual evaluation of sperm morphology. Automated normal sperm morphology outcomes also were found to be significant predictors of IVF and pregnancy in a GIFT program.
Coetzee
K
K
Department of Obstetrics and Gynaecology, Tygerberg Hospital, University of Stellenbosch, South Africa. kc@maties.sun.ac.za
de Villiers
A
A
Kruger
T F
TF
Lombard
C J
CJ
D000328:Adult; D001322:Autoanalysis / Q000295:instrumentation*; D005260:Female; D005307:Fertilization in Vitro; D015181:Gamete Intrafallopian Transfer; D006801:Humans; D016015:Logistic Models; D008297:Male; D011247:Pregnancy; D011256:Pregnancy Outcome; D018873:Pregnancy Rate; D011379:Prognosis; D011446:Prospective Studies; D012044:Regression Analysis; D013094:Spermatozoa / Q000648:ultrastructure*