Optimal detection and identification of Mycobacterium haemophilum in specimens from pediatric patients with cervical lymphadenopathy.
Acid-fast bacilli from pediatric patients with lymphadenopathy were detected in the BACTEC radiometric system and in MB Redox broth, but not on Löwenstein Jensen medium. PCR amplification identified the isolates as Mycobacterium haemophilum, which has special nutrition requirements (iron supplements) for growth. Suitable culture medium ensures optimal recovery of this microorganism, avoiding underdiagnosis.
Samra
Z
Z
Department of Microbiology, Rabin Medical Center, Petah Tiqva, Israel. HY-LABS@Internet.Zahav.net
Kaufmann
L
L
Zeharia
A
A
Ashkenazi
S
S
Amir
J
J
Bahar
J
J
Reischl
U
U
Naumann
L
L
D001483:Base Sequence; D001707:Biopsy, Needle; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007223:Infant; D008198:Lymph Nodes / Q000382:microbiology / Q000473:pathology; D008297:Male; D008969:Molecular Sequence Data; D018393:Mycobacterium haemophilum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014388:Tuberculosis, Lymph Node / Q000175:diagnosis* / Q000473:pathology