Analysis of the oligosaccharide units of xyloglucans by digestion with isoprimeverose-producing oligoxyloglucan hydrolase followed by anion-exchange chromatography.
A method for rapidly identifying six of the most commonly found xyloglucan oligosaccharide units, XXXG, XLXG, XXLG, XLLG, XXFG, and XLFG was developed by high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) before and after digestion with purified isoprimeverose-producing oligoxyloglucan hydrolase (IPase). Using this method, the compositions of oligosaccharide units of soybean and mung bean xyloglucans were re-examined. Significant amounts of oligosaccharides that have not previously been reported to be oligosaccharide units of soybean and mung bean xyloglucans were found.
Konishi
T
T
Laboratory of Food Science, Faculty of Education, Hirosaki University, Japan.
Mitsuishi
Y
Y
Kato
Y
Y
D002852:Chromatography, Ion Exchange; D004187:Disaccharides / Q000096:biosynthesis; D007887:Fabaceae / Q000737:chemistry; D005936:Glucans / Q000032:analysis*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D009844:Oligosaccharides / Q000032:analysis*; D010946:Plants, Medicinal; D013025:Glycine max / Q000737:chemistry