The enhancing effect of excess retinol palmitate on induction of odontogenic tumors and inhibitory effect on squamous cell carcinoma of the gingiva in hamsters treated with N-methylnitrosourea.
The influence of excess retinol palmitate on induction of tumors in the oral region was examined histopathologically. Sixty-three weanling Syrian golden hamsters were divided into five groups and received either 0.2% N-methylnitrosourea (MNU) (1 mg/100 g body weight) or retinol palmitate (RP) (25,000 IU/100 g body weight) twice a week for 16 weeks, singly or in combination. Animals received RP intraperitoneally or intragastrically and then, 6 hours later, the animals received intragastric administration of MNU. To accelerate the cell activity of the incisal tooth buds, intentional disocclusion of the left upper and lower incisor of all hamsters was carried out by repeated cutting with cooled diamond disks to a level just above the inter-dental papilla twice a week for 12 weeks. The right incisors were left in occlusion. In all animals exposed to RP + MNU, while the induction of squamous cell carcinomas of the gingiva and forestomach were prevented, the notable findings were a significantly increased incidence of odontogenic tumors in cut incisal regions of the animals with intragastric administration of RP + MNU and an induction of maxillary neurogenic tumors. The incidence of MNU-induced disturbances in odontogenesis in the incisors was reduced but marked disturbances were increased. RP seemed to have opposite effects of prevention and enhancement for development of neoplastic changes in the oral region.
Kohgo
T
T
Department of Oral Pathology, Hokkaido University School of Dentistry, Sapporo, Japan. kohgo@den.hokudai.ac.jp
Mezawa
F
F
Iizuka
T
T
Shindoh
M
M
D000818:Animals; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000139:chemically induced / Q000473:pathology*; D006224:Cricetinae; D004224:Diterpenes; D004336:Drug Antagonism; D004357:Drug Synergism; D005887:Gingival Neoplasms / Q000139:chemically induced / Q000473:pathology*; D008647:Mesocricetus; D008770:Methylnitrosourea / Q000494:pharmacology*; D009062:Mouth Neoplasms / Q000139:chemically induced / Q000473:pathology*; D009808:Odontogenic Tumors / Q000139:chemically induced / Q000473:pathology*; D012176:Retinoids / Q000097:blood; D000084562:Retinyl Esters; D014801:Vitamin A / Q000031:analogs & derivatives* / Q000494:pharmacology