Inhibition of transforming growth factor-beta 2 expression with phosphorothioate antisense oligonucleotides in U937 cells.
Four types of phosphorothioate antisense oligonucleotides for transforming growth factor-beta 2 were synthesized and tested for their antisense activity in U937 cells. The full-length phosphorothioate modified antisense analogues exhibited the highest inhibitory effects on the transforming growth factor-beta 2 expression in U937 cells.
Shen
Z J
ZJ
Department of Biochemistry, Kyungpook National University, Taegu, Korea.
Kim
S K
SK
Lee
Y S
YS
Lee
J W
JW
Moon
B J
BJ
D000970:Antineoplastic Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D015153:Blotting, Western; D002411:Cation Exchange Resins / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D018387:Codon, Initiator; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers; D004353:Drug Evaluation, Preclinical; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis / Q000737:chemistry; D006801:Humans; D007202:Indicators and Reagents / Q000494:pharmacology; D008055:Lipids / Q000494:pharmacology; D016376:Oligonucleotides, Antisense / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D010084:Oxidation-Reduction; D017553:Phosphorus Compounds / Q000737:chemistry; D012333:RNA, Messenger / Q000187:drug effects / Q000235:genetics; D013873:Thionucleotides / Q000737:chemistry / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism*; D020298:U937 Cells