sn-glycerol-1-phosphate-forming activities in Archaea: separation of archaeal phospholipid biosynthesis and glycerol catabolism by glycerophosphate enantiomers.
In Methanobacterium thermoautotrophicum, sn-glycerol-1-phosphate (G-1-P) dehydrogenase is responsible for the formation of the Archaea-specific backbone of phospholipids, G-1-P, from dihydroxyacetonephosphate (DHAP). The possible G-1-P-forming activities were surveyed in cell-free extracts of six species of Archaea. All the archaeal cell-free homogenates tested revealed the ability to form G-1-P from DHAP. In addition, activities of G-3-P-forming glycerol kinase and G-3-P dehydrogenase were also detected in four heterotrophic archaea, while glycerol kinase activity was not detected in two autotrophic methanogens. These results show that G-1-P is produced from DHAP by G-1-P dehydrogenase in a wide variety of archaea while exogenous glycerol is catabolized via G-3-P.
Nishihara
M
M
Department of Chemistry, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
Yamazaki
T
T
Oshima
T
T
Koga
Y
Y
D004099:Dihydroxyacetone Phosphate / Q000378:metabolism*; D019605:Euryarchaeota / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000032:analysis; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000032:analysis; D005994:Glycerophosphates / Q000096:biosynthesis / Q000378:metabolism*; D008954:Models, Biological; D010446:Peptide Fragments / Q000032:analysis; D010743:Phospholipids / Q000096:biosynthesis*; D013237:Stereoisomerism