Epitope specificities and antibody responses to the EG95 hydatid vaccine.
Antibody isotype and epitope specificities were examined in sheep immunized with EG95, a protective recombinant vaccine against hydatid disease. All sheep immunized with EG95 as a fusion protein with glutathione S-transferase (GST) produced prominent IgG antibodies against the EG95 portion of the protein. Linear, antibody-binding epitope specificities of EG95 were mapped using a series of 25 overlapping synthetic peptides. Three immunodominant regions were identified which generated specific IgG1 and IgG2 antibodies in the majority of vaccinated sheep. These regions corresponded to the EG95-derived sequences SLKAVNPSDPLVYKRQTAKF, DIETPRAGKKESTVMTSGSA and SALTSAIAGFVFSC. An additional immunogenic region was identified which induced almost exclusively IgG2 antibody. This epitope was located within the sequence TETPLRKHFNLTPV. The anti-parasitic, protective effects of the EG95 vaccine correlated with the detection of specific antibody to two or more of the four linear immunogenic regions. The identification of these immunogenic peptides of EG95 maybe useful in the development of a synthetic peptide vaccine as a derivative of the EG95 recombinant.
Woollard
D J
DJ
Molecular Parasitology Laboratory, The University of Melbourne, Werribee, Victoria, Australia.
Gauci
C G
CG
Heath
D D
DD
Lightowlers
M W
MW
D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology; D000918:Antibody Specificity / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology / Q000378:metabolism; D004443:Echinococcosis / Q000517:prevention & control / Q000662:veterinary*; D004446:Echinococcus / Q000276:immunology*; D018604:Epitope Mapping; D016056:Immunodominant Epitopes / Q000276:immunology*; D007132:Immunoglobulin Isotypes; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011993:Recombinant Fusion Proteins; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology*