title: Myelin basic protein and myelin basic protein peptides induce the proliferation of Schwann cells via ganglioside GM1 and the FGF receptor.
abstract: Myelin basic protein (MBP) and two peptides derived from MBP (MBP(1-44) and MBP(152-167)) stimulated Schwann cell (SC) proliferation in a cAMP-mediated process. The two mitogenic regions of MBP did not compete with one another for binding to SC suggesting a distinctive SC receptor for each mitogenic peptide. Neutralizing antibodies to the fibroblast growth factor receptor blocked the mitogenic effect of the myelin-related SC mitogen found in the supernatant of myelin-fed macrophages. The binding of 125I-MBP to Schwann cells was specifically inhibited by basic fibroblast growth factor (bFGF) and conversely the binding of 125I-bFGF was competitively inhibited by MBP. These data suggested that the mitogenic effect of one MBP peptide was mediated by a bFGF receptor. The binding of MBP to ganglioside GM1 and the ability of MBP peptides containing homology to the B subunit of cholera toxin (which binds ganglioside GM1) to compete for the binding of a mitogenic peptide (MBP(1-44)) to SC, identified ganglioside GM1 as a second SC receptor. Based on these results, we conclude that MBP(1-44) and MBP(152-167) associate with ganglioside GM1 and the bFGF receptor respectively to stimulate SC mitosis.
authors.affiliation: Research Service, Hines/VA Hospital, Illinois 60141, USA.
authors.forename: S F
authors.initials: SF
authors.lastname: Tzeng
authors.forename: G E
authors.initials: GE
authors.lastname: Deibler
authors.forename: G H
authors.initials: GH
authors.lastname: DeVries
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005677:G(M1) Ganglioside / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D008934:Mitogens / Q000494:pharmacology*; D004676:Myelin Basic Protein / Q000737:chemistry / Q000494:pharmacology*; D010446:Peptide Fragments / Q000494:pharmacology*; D017468:Receptors, Fibroblast Growth Factor / Q000378:metabolism / Q000502:physiology*; D012583:Schwann Cells / Q000166:cytology / Q000187:drug effects*