Light and electron microscopic examination of exocrine pancreas using zinc iodide-osmium tetroxide technique.
Zinc iodide-osmium tetroxide (ZIO) fixation/staining technique is a metallophilic technique which has been used for the examination of various tissues and cell types. We examined the ZIO (+) cell types in rat exocrine pancreas to obtain further evidence for the significance of the reaction. Among mostly non-reactive pancreatic acinar cells there were ZIO (+) acinar cells of varying staining intensity. Zymogenic granules and centroacinar cells were completely non-reactive. Our electron microscopic findings support the view that the reactivity of the technique used is cell specific but not cell type or organelle specific.
Atilla
P
P
Hacettepe University Faculty of Medicine, Department of Histology and Embryology, Ankara.
Akkuş
M
M
Deveci
E
E
Bilgin
Y
Y
Inalöz
S
S
Dağdeviren
A
A
D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005404:Fixatives; D007454:Iodides / Q000737:chemistry; D008854:Microscopy, Electron / Q000379:methods; D009993:Osmium Tetroxide / Q000737:chemistry; D010179:Pancreas / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D013194:Staining and Labeling / Q000379:methods; D016707:Tissue Fixation / Q000379:methods*; D017967:Zinc Compounds / Q000737:chemistry