Apoptosis induced by niacin-related compounds in HL-60 cells.
We have investigated whether niacin-related compounds act as inducers of apoptosis in HL-60 cells. In this study, we found that picolinic acid, dipicolinic acid, and isonicotinamide strongly induce apoptosis. After treatments with these compounds, apoptosis started within 4 h and was induced in about 50% of the cells within 8 h. These compounds induced apoptosis at 5-10 mM, but did not at 1 mM. An ICE-like protease inhibitor (Z-Asp-CH2-DCB) completely blocked the apoptosis, but a caspase-1 inhibitor (Ac-YVAD-CHO) and a caspase-3 inhibitor (Ac-DEVD-CHO) did not block the apoptosis, suggesting that other caspases have the critical roles in the execution process of apoptosis induced by niacin-related compounds.
Ogata
S
S
Laboratory of Biological Chemistry, Faculty of Bioresouces, Mie University, Japan.
Takeuchi
M
M
Okumura
K
K
Taguchi
H
H
D017209:Apoptosis / Q000187:drug effects*; D061945:Caspase Inhibitors; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005434:Flow Cytometry; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects; D006801:Humans; D007502:Iron Chelating Agents / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D009525:Niacin / Q000737:chemistry / Q000494:pharmacology*; D009536:Niacinamide / Q000494:pharmacology; D010848:Picolinic Acids / Q000494:pharmacology