title: Characteristics of gene 28 product, the constituent of the central part of bacteriophage T4 baseplate.
abstract: The phage TD gene 28 product has been partially characterized and its biological role has been examined. It was found to be a protein with a molecular size of 24 kDa which cosediments with the membrane fraction of the bacterial extracts and could only be washed out by a 0.2% sarcosyl solution. Other observations indicate that gp 28 has a majority of hydrophobic residues on its surface and forms a homotrimeric complex in the absence of other phage proteins. The product was finally identified as a baseplate structural component. Incubation of purified phage preparation in a buffer which contained active protein 28, did not affect the efficiency of the plating. However, incubation of the phage particles with specific antiserum was found to neutralize phage infectivity.
authors.affiliation: Department of Microbiology, University of Gdańsk, Poland. nieradko@biotech.univ.gda.pl
authors.forename: J
authors.initials: J
authors.lastname: Nieradko
authors.forename: P
authors.initials: P
authors.lastname: Koszałka
authors.forename: A
authors.initials: A
authors.lastname: Krzywicka
mesh_terms: D017122:Bacteriophage T4 / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000821:virology*; D005814:Genes, Viral; D005816:Genetic Complementation Test; D015151:Immunoblotting; D009500:Neutralization Tests; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly