Specific effects of a recB mutation on the HfrH strain of Escherichia coli.
The recB268::Tn10 mutation was introduced into the HfrH strain of Escherichia coli. Compared with recB F- and recB F+ cells, the viability of this mutant strain was much lower. Compared with wild-type HfrH, the recB derivative donated much shorter fragments of its chromosome to the recipient. It is suggested that the recB gene product (i.e., RecBCD enzyme) participates in Hfr transfer.
Dermić
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Ruer Bosković Institute, 10001 Zagreb, Croatia. dermic@rudjer.irb.hr
Trgovcević
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D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D043211:Exodeoxyribonuclease V; D005090:Exodeoxyribonucleases / Q000235:genetics*; D005144:F Factor / Q000235:genetics*; D009154:Mutation