Immune regulatory loops determine productive interactions within human T lymphocyte-dendritic cell clusters.
Help for the induction of cytolytic T lymphocytes is mediated by dendritic cells (DC) that are conditioned by CD40 signaling. We identified tumor necrosis factor family member CD27L/CD70, which is expressed by cytolytic T lymphocytes on interaction with DC to control CD154 (CD40L) up-regulation on CD45RA+ helper T cells for subsequent DC stimulation. The results show that the initiation of a cytolytic immune response is determined by regulatory circuits, requiring simultaneous activation and differentiation of all cells involved in T lymphocyte-DC cluster formation.
Stuhler
G
G
University of Tübingen, Medical Clinic II, Otfried-Müller Strasse 10, 72076 Tübingen, Germany.
Zobywalski
A
A
Grünebach
F
F
Brossart
P
P
Reichardt
V L
VL
Barth
H
H
Stevanovic
S
S
Brugger
W
W
Kanz
L
L
Schlossman
S F
SF
D000818:Animals; D015703:Antigens, CD / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology; D053282:CD27 Ligand; D019013:CD40 Antigens / Q000276:immunology*; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D015789:HLA-A2 Antigen / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation; D017493:Leukocyte Common Antigens / Q000276:immunology; D008024:Ligands; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D011971:Receptors, Immunologic / Q000276:immunology; D011994:Recombinant Proteins / Q000096:biosynthesis; D015398:Signal Transduction; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D014162:Transfection