title: Differential gene expression in neoplastic and human papillomavirus-immortalized oral keratinocytes.
abstract: We have previously demonstrated that normal human oral keratinocytes immortalized by transfection with human papillomavirus type-16 Dna became tumorigenic after exposure to a chemical carcinogen. In an effort to detect differentially regulated genes associated with this transition from the immortal to the malignant phenotype, we employed representational differences analysis (a PCR-coupled subtractive hybridization technique). After analysing 50 colonies, 12 putative messages were identified. Northern analysis comparison using the identified cDNAs as probes was made between normal human oral keratinocyte, papillomavirus-immortalized human oral keratinocytes (HOK-16B), a neoplastic cell line derived from HOK-16B (HOK-16B-BaP-T) and the human oral cancer cell lines Hep-2, SCC-9 and Tu-177. We found that mRNAs encoding for cyclophilin A, c-myc binding protein 1, the heat shock protein 90alpha and one unknown transcript were up-regulated in the oral cancer cell lines analysed as well as in HOK-16B cells. We also detected a downregulation of the mRNAs encoding the skin-derived antileukoproteinase SKALP/elafin, the translationally regulated p23 protein and one unknown transcript. Whether these messages are associated to the neoplastic conversion of human keratinocytes remains to be determined.
authors.affiliation: UCLA School of Dentistry and Dental Research Institute, Los Angeles, California 90095-1668, USA.
authors.forename: O
authors.initials: O
authors.lastname: Rey
authors.forename: M A
authors.initials: MA
authors.lastname: Baluda
authors.forename: N H
authors.initials: NH
authors.lastname: Park
mesh_terms: D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015603:Keratinocytes; D009055:Mouth; D027383:Papillomaviridae / Q000502:physiology*; D014407:Tumor Cells, Cultured