Gassericin A; an uncommon cyclic bacteriocin produced by Lactobacillus gasseri LA39 linked at N- and C-terminal ends.
Gassericin A, a bacteriocin that was produced by Lactobacillus gasseri LA39, was treated with lysylendopeptidase and 3-bromo-3-methyl-2-(2-nitrophenyl-mercapto)-3H-indole. The fragments were recovered by SDS-PAGE and sequenced. All amino acids of gassericin A were distributed by sequence analysis and the bacteriocin did not contain any modified amino acids. The amino acid sequence of gassericin A completely coincided with that found through the cloning of the structural gene. Gassericin A was shown to be a cyclic bacteriocin (class II) which is bound at the N- and C-terminal ends. This is the first example of a cyclic bacteriocin from lactobacilli lactic acid bacteria.
Kawai
Y
Y
Laboratory of Animal Products Chemistry, Biological Resource Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. ykawai@bios.tohoku.ac.jp
Saito
T
T
Kitazawa
H
H
Itoh
T
T
D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007211:Indoles / Q000737:chemistry; D007779:Lactobacillus acidophilus / Q000378:metabolism*; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000096:biosynthesis; D017421:Sequence Analysis; D012697:Serine Endopeptidases / Q000737:chemistry