HIV-1 gp160 decreases the K+ voltage-gated current from Jurkat E6.1 T cells by up-phosphorylation.
HIV-1 gp120/gp160 is known to disturb the activity of p56lck, protein kinase C (PKC) and Ca2+ homeostasis in T lymphocytes. We found that gp160 decreases the Kv1.3 current of Jurkat E6.1 cells probably by increasing the PKC-dependent phosphorylation of Kv channel protein after 5 days. This decrease is dose-dependent. In contrast, gp160 did not decrease the Kv1.3 current of the JCaM1.6 cell line, a p56(lck)-defective Jurkat cell line. This shows that p56lck was at the beginning of the events which induced the Kv1.3 current decrease. As a consequence of this decrease, Jurkat E6.1 cells were depolarized and exhibited a volume increase.
Dellis
O
O
Laboratoire d'Electrophysiologie des Membranes, LPCMSP, Université Denis Diderot, Paris 7, France. odellis@moka.ccr.jussieu.fr
Bouteau
F
F
Guenounou
M
M
Rona
J P
JP
D019302:HIV Envelope Protein gp160 / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D015640:Ion Channel Gating; D019169:Jurkat Cells; D051664:Kv1.3 Potassium Channel; D010766:Phosphorylation; D026902:Potassium Channel Blockers; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated