Evidence for protein splicing in the endoplasmic reticulum-Golgi intermediate compartment.
We present evidence that the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) is a site for protein splicing of cathepsin C: (i) maturation of the enzyme in COS 7 cells is a two-step process starting within the ERGIC, and (ii) the intermediately processed polypeptide retains both termini of the proenzyme and lacks an internal fragment.
Demirov
D
D
Department of Chemistry and Biochemistry, Medical University, Sofia, Bulgaria.
Sarafian
V
V
Kremensky
I
I
Ganev
V
V
D000818:Animals; D001665:Binding Sites / Q000235:genetics; D019556:COS Cells; D020789:Cathepsin C; D002451:Cell Compartmentation; D004152:Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / Q000235:genetics / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D004792:Enzyme Precursors / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D006056:Golgi Apparatus / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014162:Transfection