Adhesive and growth properties of lectin from the ascidian Didemnum ternatanum on cultivated marine invertebrate cells.
The effects of N-Acetyl-D-glucosamine-specific lectin (M(r) 27 kDa) isolated from the ascidian Didemnum ternatanum on cultivated cells of molluscs and echinoderms were studied. This lectin was found to stimulate the growth or the differentiation of cultivated marine invertebrate cells depending on the stage of embryonic development at which primary cell cultures were obtained. In addition, it has been shown to increase the attachment of cells in primary cultures of these animals. The degree of attachment is considerably increased when collagen or polylysine substrates are used. Using scanning electron microscopy we have demonstrated the stage-specific effect of this lectin on embryonic sea urchin and molluscan cells. Intensive cell spreading and an alteration of cell shape were observed only at the gastrula stage, when the switching from maternal information to embryonic genes occurred. The ascidian lectin seems to have some characteristics of both an adhesive factor and a growth factor.
Odintsova
N A
NA
Institute of Marine Biology, Far East Branch of RAS, Vladivostok, Russia. aisa@vld.global-one.ru
Belogortseva
N I
NI
Ermak
A V
AV
Molchanova
V I
VI
Luk'yanov
P A
PA
D000818:Animals; D049872:Bivalvia / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002448:Cell Adhesion / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D007700:Kinetics; D037102:Lectins / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D012617:Sea Urchins / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014561:Urochordata / Q000737:chemistry