title: delta-catenin, an adhesive junction-associated protein which promotes cell scattering.
abstract: The classical adherens junction that holds epithelial cells together consists of a protein complex in which members of the cadherin family linked to various catenins are the principal components. delta-catenin is a mammalian brain protein in the Armadillo repeat superfamily with sequence similarity to the adherens junction protein p120(ctn). We found that delta-catenin can be immunoprecipitated as a complex with other components of the adherens junction, including cadherin and beta-catenin, from transfected cells and brain. The interaction with cadherin involves direct contact within the highly conserved juxtamembrane region of the COOH terminus, where p120(ctn) also binds. In developing mouse brain, staining with delta-catenin antibodies is prominent towards the apical boundary of the neuroepithelial cells in the ventricular zone. When transfected into Madin-Darby canine kidney (MDCK) epithelial cells delta-catenin colocalized with cadherin, p120(ctn), and beta-catenin. The Arm domain alone was sufficient for achieving localization and coimmunoprecipitation with cadherin. The ectopic expression of delta-catenin in MDCK cells altered their morphology, induced the elaboration of lamellipodia, interfered with monolayer formation, and increased scattering in response to hepatocyte growth factor treatment. We propose that delta-catenin can regulate adhesion molecules to implement the organization of large cellular arrays necessary for tissue morphogenesis.
authors.affiliation: Center for Neurologic Diseases, Department of Neurology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Lu
authors.forename: M
authors.initials: M
authors.lastname: Paredes
authors.forename: M
authors.initials: M
authors.lastname: Medina
authors.forename: J
authors.initials: J
authors.lastname: Zhou
authors.forename: R
authors.initials: R
authors.lastname: Cavallo
authors.forename: M
authors.initials: M
authors.lastname: Peifer
authors.forename: L
authors.initials: L
authors.lastname: Orecchio
authors.forename: K S
authors.initials: KS
authors.lastname: Kosik
mesh_terms: D000818:Animals; D051186:Armadillo Domain Proteins; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D001921:Brain / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D051177:Catenins; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D048429:Cell Size / Q000502:physiology; D003598:Cytoskeletal Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D017931:DNA Primers / Q000235:genetics; D004285:Dogs; D029721:Drosophila Proteins; D006801:Humans; D019476:Insect Proteins / Q000737:chemistry / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D010750:Phosphoproteins; D015534:Trans-Activators; D014162:Transfection; D051176:beta Catenin; D000095002:Delta Catenin