title: Angiogenesis and expression of tenascin after transmural laser revascularization.
abstract: Transmyocardial revascularization (TMR) with CO2-laser equipment is an alternative approach in the treatment of patients with severe ischemic cardiac disease. Several studies concerning morphological features after TMR document a strong transmyocardial injury, but little is known about wound healing in laser-induced alterations of the cardiac skeleton and their putative role for angiogenesis and endothelialization. The present study was conducted to establish a useful immunohistochemical marker for detection of these laser-induced injuries and to analyze starting points of angiogenesis in human myocardium after TMR. Our data show that tenascin labeling is a useful immunohistochemical approach to detect laser-alterated segments of the cardiac skeleton as well as laser-induced fibrosis. Starting points of the angiogenetic process are seen throughout the margins of laser-induced lesions where myocardial capillaries are found. Disrupted vessels located within laser-alterated connective tissue septa are not major starting points for endothelialization of laser-induced lesions and for capillary sprouts. In comparison to laser-induced fibrosis, induction and promotion of angiogenesis by laser radiation is weak.
authors.affiliation: Institute for Anatomy and Cell Biology I, University of Heidelberg, Germany.
authors.forename: N
authors.initials: N
authors.lastname: Gassler
authors.forename: F
authors.initials: F
authors.lastname: Rastar
authors.forename: M W
authors.initials: MW
authors.lastname: Hentz
mesh_terms: D003331:Coronary Vessels / Q000378:metabolism* / Q000473:pathology; D006321:Heart / Q000528:radiation effects*; D006801:Humans; D053685:Laser Therapy; D007834:Lasers / Q000009:adverse effects; D017202:Myocardial Ischemia / Q000532:radiotherapy; D009204:Myocardial Revascularization / Q000379:methods; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D009389:Neovascularization, Pathologic; D012863:Skeleton; D019063:Tenascin / Q000096:biosynthesis*; D013997:Time Factors