"High-load" polyethylene glycol-polystyrene (PEG-PS) graft supports for solid-phase synthesis.
The choice of a polymeric support is a key factor for the success of solid-phase methods for syntheses of organic compounds and biomolecules such as peptides and oligonucleotides. Classical Merrifield solid-phase peptide synthesis (SPPS), performed on low cross-linked hydrophobic polystyrene (PS) beads, sometimes suffers from sequence-dependent coupling difficulties. The concept of incorporating polyethylene glycol (PEG) into supports for solid-phase synthesis represents a successful approach to alleviating such problems. Previous reports from our laboratories have shown the advantages of "low-load" PEG-PS (0.15-0.25 mmol/g) for SPPS. Herein, we demonstrate that the beneficial aspects of the PEG-PS concept can be extended with resins that have higher loadings (0.3-0.5 mmol/g).
Kates
S A
SA
PerSeptive Biosystems, Inc., Framingham, MA 01701, USA.
McGuinness
B F
BF
Blackburn
C
C
Griffin
G W
GW
Solé
N A
NA
Barany
G
G
Albericio
F
F
D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000138:chemical synthesis; D002625:Chemistry, Organic / Q000379:methods; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry*; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry