Phosphoinositide 3-kinase activity is essential for all-trans-retinoic acid-induced granulocytic differentiation of HL-60 cells.
Phosphoinositide 3-kinase (PI 3-K) activity increases in HL-60 cells that are induced to granulocytic differentiation by all-trans-retinoic acid. Immunochemical and immunocytochemical analyses by confocal microscopy also reveal an increase in the amount of the enzyme, which is particularly evident at the nuclear level. Inhibition of PI 3-K activity by nanomolar concentrations of wortmannin and of its expression by transfection with an antisense fragment of p85alpha prevented the differentiative process. The data obtained indicate that PI 3-K activity plays an essential role in promoting granulocytic differentiation.
Bertagnolo
V
V
Department of Morphology and Embryology, University of Ferrara, Italy.
Neri
L M
LM
Marchisio
M
M
Mischiati
C
C
Capitani
S
S
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D015536:Down-Regulation; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D018922:HL-60 Cells / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D018613:Microscopy, Confocal; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism; D013347:Subcellular Fractions / Q000201:enzymology; D014212:Tretinoin / Q000494:pharmacology*