title: Phospholipase D-neutralization in serodiagnosis of Arcanobacterium haemolyticum and Corynebacterium pseudotuberculosis infections.
abstract: Phospholipase D (PLD) neutralization was used to examine sera of humans (n = 40) with a spontaneous infection by Arcanobacterium haemolyticum, sheep and goats (n = 76 and 79 respectively) with a spontaneous infection by Corynebacterium pseudotuberculosis, mice (n = 26) experimentally immunized with PLD from A. haemolyticum (PLD-A) and mice (n = 28) experimentally immunized with PLD from C. pseudotuberculosis (PLD-C). PLD-A and PLD-C were also used as neutralizing antigens. A positive result of neutralization was due to an inhibition of the haemolytic synergism with the equi factor from Rhodococcus equi. The titres of sera neutralizing the homologous PLD were always significantly higher than those neutralizing the heterologous PLD. The proportion of sera that were able to neutralize the homologous PLD in sheep, goats and mice immunized with PLD-A significantly exceeded the proportion of sera that neutralized the heterologous PLD. The antigenic properties of PLD-A and PLD-C were similar but not identical.
authors.affiliation: Department of Microbiology, Masaryk University Medical School, Brno, Czech Republic.
authors.forename: B
authors.initials: B
authors.lastname: Skalka
authors.forename: I
authors.initials: I
authors.lastname: Literák
authors.forename: P
authors.initials: P
authors.lastname: Chalupa
authors.forename: M
authors.initials: M
authors.lastname: Votava
mesh_terms: D000192:Actinomycetales / Q000201:enzymology* / Q000276:immunology; D000193:Actinomycetales Infections / Q000175:diagnosis* / Q000382:microbiology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D003354:Corynebacterium Infections / Q000175:diagnosis / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000201:enzymology* / Q000276:immunology; D015511:Goat Diseases / Q000175:diagnosis / Q000382:microbiology; D006041:Goats; D006801:Humans; D051379:Mice; D009500:Neutralization Tests; D010739:Phospholipase D / Q000276:immunology* / Q000378:metabolism; D012698:Serologic Tests; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis / Q000382:microbiology