Effects of pulsed magnetic stimulation of GFAP levels in cultured astrocytes.
The present study evaluates the physiological effects of magnetic stimulation on astrocyte cultures. Cell cultures were exposed to pulsed magnetic stimulation (10 Hz, 10 sec) at the following levels: 0.10 tesla (T; Group A); 0.21 T (Group B); 0.42 T (Group C); and 0.63 T (Group D). Glial fibrillary acidic protein (GFAP) levels from immunoblots, total protein concentrations, and cellular morphology were analyzed at 0, 1, 3, 5, 7, 13, and 20 days poststimulation. Significantly higher GFAP levels were observed in Group D at day 3 (P = 0.0114). The change was transient as the GFAP levels quickly returned to control levels by day 5. No other significant changes in GFAP levels were observed. In comparison to control protein levels at day 0, concentrations from Groups B, C, and D were significantly lower (P < 0.006), whereas at day 3, Groups C and D were significantly higher (P < 0.02). Differences in astrocyte morphology due to magnetic stimulation were not observed. This study demonstrated that high intensity magnetic stimulation for only 10 sec induced a transient biological response.
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Spinal Cord Injury Service, VA Palo Alto Health Care System, California, USA.
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Lee
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D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000528:radiation effects* / Q000648:ultrastructure; D015153:Blotting, Western; D002478:Cells, Cultured; D004574:Electromagnetic Fields; D005904:Glial Fibrillary Acidic Protein / Q000096:biosynthesis*; D015151:Immunoblotting; D007150:Immunohistochemistry; D017628:Microglia / Q000378:metabolism / Q000528:radiation effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis; D051381:Rats; D013997:Time Factors