title: Identification of contaminating fungal DNA sequences in Zymolyase.
abstract: When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.
authors.affiliation: Hygiene Institute, Children's Hospital, University of Heidelberg, Heidelberg, Germany. dagmar_rimek@ukl.uniheidelberg.de
authors.forename: D
authors.initials: D
authors.lastname: Rimek
authors.forename: A P
authors.initials: AP
authors.lastname: Garg
authors.forename: W H
authors.initials: WH
authors.lastname: Haas
authors.forename: R
authors.initials: R
authors.lastname: Kappe
mesh_terms: D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D004340:Drug Contamination; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D007716:Kluyveromyces / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics / Q000302:isolation & purification*