IgG subclass switching is associated with the severity of experimental autoimmune encephalomyelitis induced with myelin oligodendrocyte glycoprotein peptide in NOD mice.
We have recently shown that a single dose of the myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 produces a relapsing-remitting demyelinating disease similar to multiple sclerosis (MS) in Lewis rats. In this study we have assessed the possibility that a subclass of anti-MOG35-55 antibodies influences the clinical outcome of these diseases by examining the classes and isotypes of anti-MOG35-55 antibody produced during the course of MOG35-55-induced demyelinating disease in NOD mice. Following immunization, 7 of the 21 injected mice had only mild diseases, while the 14 others had severe progressive and/or relapsing-remitting diseases. There were no differences in anti-MOG35-55 IgG, IgA, IgM, IgG1, IgG2a, and IgG3 antibody titers between the severe and mild symptoms groups. High levels of IgG2b antibody to MOG35-55 were detected in all mice with severe symptoms. In contrast, none of the mice which contracted a mild disease produced anti-MOG35-55 IgG2b. These results suggest that in NOD mice, the IgG2b antibody response to MOG35-55 is associated with the severity of this MS-like demyelinating disease.
Ichikawa
M
M
Department of Pediatrics, Department of Medicine (Neurology), Department of Pathology, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390, Japan.
Koh
C S
CS
Inaba
Y
Y
Seki
C
C
Inoue
A
A
Itoh
M
M
Ishihara
Y
Y
Bernard
C C
CC
Komiyama
A
A
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000097:blood; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D005260:Female; D016056:Immunodominant Epitopes / Q000276:immunology; D017578:Immunoglobulin Class Switching; D007074:Immunoglobulin G / Q000235:genetics*; D007132:Immunoglobulin Isotypes / Q000097:blood; D051379:Mice; D016688:Mice, Inbred NOD; D008969:Molecular Sequence Data; D009103:Multiple Sclerosis / Q000276:immunology*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000276:immunology*; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments / Q000276:immunology*; D013116:Spinal Cord / Q000473:pathology; D014611:Vaccination