Chemical and enzymatic synthesis of glycoconjugates. 5: One-pot regioselective synthesis of bioactive galactobiosides using a CLONEZYME thermophilic glycosidase library.
Enzymatic synthesis of galactobiosides using a versatile CLONEZYME thermostable glycosidase library was studied. One-pot transglycosylation reactions were demonstrated to synthesize beta(1-->4), beta(1-->6), and alpha(1-->6) disaccharide sequences with high regioselectivity and moderate to high yields.
Li
J
J
Department of Chemistry, Wayne State University, Detroit, MI 48202-3489, USA.
Robertson
D E
DE
Short
J M
JM
Wang
P G
PG
D000951:Antigens, Neoplasm / Q000737:chemistry; D002236:Carbohydrate Conformation; D004187:Disaccharides / Q000138:chemical synthesis / Q000378:metabolism; D005697:Galactosides / Q000138:chemical synthesis / Q000378:metabolism; D006001:Glycoconjugates / Q000138:chemical synthesis* / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D006031:Glycosylation; D017261:Glycosylphosphatidylinositols / Q000138:chemical synthesis; D009844:Oligosaccharides / Q000138:chemical synthesis* / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism