Application of dried blood spot specimens for serologic subtyping of human immunodeficiency virus type 1 in Thailand.
Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.
Chanbancherd
P
P
Army Institute of Pathology, Bangkok, Thailand.
Brown
A E
AE
Trichavaroj
R
R
Tienamporn
P
P
Puthakird
P
P
Limpairojn
N
N
VanCott
T C
TC
de Souza
M S
MS
D000163:Acquired Immunodeficiency Syndrome / Q000097:blood* / Q000821:virology; D000595:Amino Acid Sequence; D001800:Blood Specimen Collection / Q000379:methods; D015483:HIV Antibodies / Q000097:blood; D015488:HIV Antigens / Q000737:chemistry / Q000276:immunology; D015497:HIV-1 / Q000145:classification* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods; D013785:Thailand