title: Ontogeny of humoral immunity in northern elephant seal (Mirounga angustirostris) neonates.
abstract: Northern elephant seal (NES) serum concentrations of total immunoglobulin (Ig) G, an IgG sub-class, and an IgM-like protein were determined by capture immunoassay using three monoclonal antibodies with specificities for Ig of members of the Phocidae pinniped family. These assays were calibrated for use with NES sera using affinity column purified Ig. Concentrations of these Ig populations were estimated in adult female sera sampled at two time points during the lactation period, as well as sera from their pups collected during the first 5 weeks after birth. In pups, concentrations of the IgM-like protein was found to increase rapidly post-partum. In some individuals, values reached mean concentrations within 10-14 days. In addition, rapid increases in pup total IgG and IgG sub-class concentrations were also observed. Collectively, these findings suggest that the majority of post-partum increases in serum Ig can be accounted for by de-novo synthesis.
authors.affiliation: Division of Clinical Sciences, Old Medical School, University of Leeds, UK. dpking@ucdavis.edu
authors.forename: D P
authors.initials: DP
authors.lastname: King
authors.forename: J L
authors.initials: JL
authors.lastname: Sanders
authors.forename: C T
authors.initials: CT
authors.lastname: Nomura
authors.forename: R A
authors.initials: RA
authors.lastname: Stoddard
authors.forename: C L
authors.initials: CL
authors.lastname: Ortiz
authors.forename: S W
authors.initials: SW
authors.lastname: Evans
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000911:Antibodies, Monoclonal; D000917:Antibody Formation; D003126:Colostrum / Q000276:immunology; D003429:Cross Reactions; D005260:Female; D007112:Immunity, Maternally-Acquired; D007118:Immunoassay / Q000379:methods / Q000706:statistics & numerical data; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood / Q000145:classification; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D011247:Pregnancy; D015203:Reproducibility of Results; D046048:Seals, Earless / Q000254:growth & development* / Q000276:immunology*