Comparison of CHROMagar Salmonella medium and hektoen enteric agar for isolation of salmonellae from stool samples.
CHROMagar Salmonella (CAS), a new chromogenic medium, was retrospectively compared to Hektoen enteric agar (HEA) with 501 Salmonella stock isolates and was then prospectively compared to HEA for the detection and presumptive identification of Salmonella spp. with 508 stool samples before and after enrichment. All stock cultures (100%), including cultures of H2S-negative isolates, yielded typical mauve colonies on CAS, while 497 (99%) isolates produced typical lactose-negative, black-centered colonies on HEA. Following overnight incubation at 37 degreesC, a total of 20 Salmonella strains were isolated from the 508 clinical samples. Sensitivities for primary plating and after enrichment were 95% (19 isolates) and 100% (20 isolates), respectively, for CAS and 80% (16 isolates) and 100% (20 isolates), respectively, for HEA. The specificity of CAS (88.9%) was significantly higher than that of HEA (78.5%; P < 0.0001). On the basis of its good sensitivity and specificity, CAS medium can be recommended for use for primary plating when human stool samples are screened for Salmonella spp.
Gaillot
O
O
Laboratoire de Bactériologie-Virologie, Hôpital Boucicaut, 75730 Paris, France. gaillot@necker.fr
di Camillo
P
P
Berche
P
P
Courcol
R
R
Savage
C
C
D000362:Agar; D001431:Bacteriological Techniques; D003470:Culture Media; D005243:Feces / Q000382:microbiology*; D006801:Humans; D012189:Retrospective Studies; D012475:Salmonella / Q000145:classification / Q000254:growth & development* / Q000302:isolation & purification; D012480:Salmonella Infections / Q000382:microbiology*; D012680:Sensitivity and Specificity