title: A structural comparison of molybdenum cofactor-containing enzymes.
abstract: This work gives an overview of the recent achievements which have contributed to the understanding of the structure and function of molybdenum and tungsten enzymes. Known structures of molybdo-pterin cofactor-containing enzymes will be described briefly and the structural differences between representatives of the same and different families will be analyzed. This comparison will show that the molybdo-pterin cofactor-containing enzymes represent a very heterogeneous group with differences in overall enzyme structure, cofactor composition and stoichiometry, as well as differences in the immediate molybdenum environment. Two recently discovered molybdo-pterin cofactor-containing enzymes will be described with regard to molecular and EPR spectroscopic properties, pyrogallol-phloroglucinol transhydroxylase from Pelobacter acidigallici and acetylene hydratase from Pelobacter acetylenicus. On the basis of its amino acid sequence, transhydroxylase can be classified as a member of the dimethylsulfoxide reductase family, whereas classification of the tungsten/molybdenum-containing acetylene hydratase has to await the determination of its amino acid sequence.
authors.affiliation: Department of Pharmacological Sciences, School of Medicine, SUNY Stony Brook, NY 11794-8651, USA.
authors.forename: C
authors.initials: C
authors.lastname: Kisker
authors.forename: H
authors.initials: H
authors.lastname: Schindelin
authors.forename: D
authors.initials: D
authors.lastname: Baas
authors.forename: J
authors.initials: J
authors.lastname: Rétey
authors.forename: R U
authors.initials: RU
authors.lastname: Meckenstock
authors.forename: P M
authors.initials: PM
authors.lastname: Kroneck
mesh_terms: D001421:Bacteria, Anaerobic / Q000201:enzymology*; D003067:Coenzymes / Q000737:chemistry; D006801:Humans; D006836:Hydro-Lyases / Q000737:chemistry*; D008667:Metalloproteins / Q000737:chemistry*; D006899:Mixed Function Oxygenases / Q000737:chemistry*; D008982:Molybdenum; D000090006:Molybdenum Cofactors; D010088:Oxidoreductases / Q000737:chemistry*; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry; D011621:Pteridines / Q000737:chemistry*; D014414:Tungsten / Q000737:chemistry; D014969:Xanthine Oxidase / Q000737:chemistry