title: Effect of chemical nutrients on aconitase activity during citric acid fermentation by a mutant strain of Aspergillus niger.
abstract: An ethyleneimine and UV irradiated mutant strain Aspergillus niger AB 1801 was explored for its aconitase activity, citric acid production and cell growth during citric acid fermentation. 10% sucrose and 0.25% urea served as the best carbon and nitrogen sources, respectively. Fe2+ stimulated aconitase activity and Co2+ (upto 5 micrograms/mL) and Ni2+ (10 micrograms/mL) strongly depressed enzyme activity. Boric acid (0.5 mg/L) had very good stimulatory response towards citric acid production. Aconitase activity has been found to vary inversely with the citric acid production upto a certain limit after which the activity was absent in the mycelial extract. Cell growth varied with the production parameter.
authors.forename: P
authors.initials: P
authors.lastname: Ghosh
authors.forename: A K
authors.initials: AK
authors.lastname: Banik
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism*; D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D001888:Boric Acids / Q000494:pharmacology; D002244:Carbon / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects; D009154:Mutation; D013395:Sucrose / Q000378:metabolism; D014131:Trace Elements / Q000494:pharmacology; D014466:Ultraviolet Rays; D014508:Urea / Q000378:metabolism