Unexpected cross-reaction with Fusobacterium necrophorum in a PCR for detection of mycoplasmas.
Cross-reactions with Fusobacterium necrophorum were found in a PCR designed for detection of a wide range of mycoplasma species. Twenty-five strains of Fusobacterium were examined; all 14 F. necrophorum strains reacted positively, whereas all 7 Fusobacterium nucleatum strains reacted negatively. Two strains that were not F. necrophorum yielded variable results.
Jensen
J S
JS
Mycoplasma Laboratory, Neisseria Department, Statens Serum Institut, Copenhagen, Denmark. jsj@ssi.dk
Bruun
B
B
Gahrn-Hansen
B
B
D000293:Adolescent; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D016477:Artifacts; D002981:Clindamycin / Q000627:therapeutic use; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D003937:Diagnosis, Differential; D005260:Female; D005674:Fusobacterium Infections / Q000175:diagnosis* / Q000188:drug therapy; D005675:Fusobacterium necrophorum / Q000145:classification* / Q000235:genetics; D006801:Humans; D008169:Lung Abscess / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D009174:Mycoplasma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity; D016415:Sequence Alignment; D013045:Species Specificity