title: Dynamic expression of a native chondroitin sulfate epitope reveals microheterogeneity of extracellular matrix organization in the embryonic chick heart.
abstract: TC2 is a novel monoclonal antibody produced by in vitro immunization of splenocytes with a peanut agglutinin-positive fraction from extracts of prechondrogenic micromass cultures of chick limb mesenchyme. ELISA results demonstrated TC2 reactivity with a native epitope on a glycosaminoglycan (GAG) enriched in chondroitin-4-sulfate and with multiple intact proteoglycans, but not with other GAGs tested. TC2 immunohistochemical reactivity was abolished by pretreatment of sections with chondroitinase AC or preadsorption with chondroitin-4-sulfate GAG. Strong TC2 localization occurred throughout the developing heart at stage 9. As looping ensued, a graded reactivity was observed from lowest in the atrium to highest in the conotruncus that correlated well with versican localization. The superior atrioventricular cushion stained preferentially with TC2 as compared to the inferior cushion at stages 16-18. At these later stages TC2 patterns did not agree completely with anti-versican reactivity. By stage 23 there was a marked reduction in TC2 localization in the heart, however, strong reactivity remained at certain sites, including the conotruncus and in subcompartments of both atrioventricular cushions. A heterogeneous distribution of other native chondroitin sulfate glycosaminoglycan epitopes recognized by monoclonal antibodies d1C4 and CS-56 was observed as well. The distribution of the TC2 epitope usually did not overlap with d1C4 or CS-56 localization at the stages examined. Overall, the spatiotemporal characteristics of TC2 reactivity in the developing chick heart appear to correlate with subdomains of the endocardial cushions as well as with trabecular and atrial septal formation.
authors.affiliation: Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston 29425, USA. capehart@musc.edu
authors.forename: A A
authors.initials: AA
authors.lastname: Capehart
authors.forename: C H
authors.initials: CH
authors.lastname: Mjaatvedt
authors.forename: S
authors.initials: S
authors.lastname: Hoffman
authors.forename: E L
authors.initials: EL
authors.lastname: Krug
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000032:analysis / Q000378:metabolism*; D002810:Chondroitinases and Chondroitin Lyases / Q000494:pharmacology; D000939:Epitopes / Q000276:immunology*; D005109:Extracellular Matrix / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000032:analysis / Q000276:immunology; D006321:Heart / Q000196:embryology*; D006325:Heart Atria / Q000737:chemistry; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007150:Immunohistochemistry; D008648:Mesoderm / Q000276:immunology; D051379:Mice; D009206:Myocardium / Q000737:chemistry*