Structural study on a sulfated polysaccharide-peptidoglycan complex produced by Arthrobacter sp.
The structure of a sulfated polysaccharide-peptidoglycan complex (SP-PG) produced by Arthrobacter sp. was analyzed by NMR spectroscopy. In addition, oligosaccharide fragments of the SP-PG-L obtained by HF degradation were analyzed by NMR spectroscopy. These findings indicated that the sulfated polysaccharide (SP) contains a repeating unit composed of two galactofuranosides and a glucopyranoside. The main chain of the trisaccharide is [-->6) beta-D-Galf(1-->6)-beta-D-Galf(1-->ln, with beta-D-Glcp linked to one of the Galfs through a (1-->2) linkage. The sulfated positions of the trisaccharide were identified as C-3 and C-5 of the beta-glucosylated Galf residues, and C-2 or C-3 of the other Galf residue.
Yamazaki
K
K
Basic Technology Research Laboratory, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan. vamaz33x@daiichipharm.co.jp
Suzuki
M
M
Inukai
K
K
Kuga
H
H
Korenaga
H
H
D001173:Arthrobacter / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000737:chemistry*; D014312:Trisaccharides / Q000737:chemistry*