Fluorescence in situ hybridization with chromosome paint probes: a novel approach to assess aneuploidy in human sperm nuclei.
OBJECTIVE
Fluorescence in situ hybridization (FISH) using whole-chromosome paint probes was performed to evaluate disomy and diploidy frequency for chromosomes 1, 18, 19, and 22 in human sperm nuclei.


METHODS
Ten subjects of proven fertility and normal spermatic parameters were included in the study. A dual-color FISH method was carried out.


RESULTS
A total of 157,896 spermatozoa was scored. The mean frequencies of disomic sperm for chromosomes 1, 18, 19, and 22 were 0.22% (range, 0.19 to 0.28%), 0.24% (range, 0.14 to 0.37%), 0.22% (range, 0.17 to 0.30%), and 0.25% (range, 0.21 to 0.29%), respectively. The mean frequency of diploidy was 0.14% (range, 0.09 to 0.18%). No interindividual and interchromosomal variations in the aneuploidy frequency were observed between the different subjects.


CONCLUSIONS
FISH with whole-chromosome paint probes provides a novel and efficient approach for disomy assessment in human sperm nuclei.
Rives
N
N
Reproductive Biology Laboratory, Rouen University Hospital, France.
Wust
S
S
David
B
B
Duchesne
V
V
Joly
G
G
Mace
B
B
D000328:Adult; D000782:Aneuploidy; D002467:Cell Nucleus / Q000235:genetics*; D020223:Chromosome Painting; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002888:Chromosomes, Human, Pair 19 / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D004171:Diploidy; D006801:Humans; D008297:Male; D013094:Spermatozoa