Ultrastructural localization of tartrate-resistant acid phosphatase activity in rat osteoblasts.
Ultrastructural localization of tartrate-resistant acid phosphatase (TRAP) activity in metaphyseal osteoblasts of young rats was detected and compared with the localization of tartrate-sensitive acid phosphatase (TSAP). TRAP activity was detected in lysosomes and Golgi complex, such as Golgi lamellae, vacuoles and vesicles. TSAP, detected as beta-glycerophosphatase, was also located in Golgi lamellae, vacuoles and vesicles, and in lysosomes. Based on the ultrastructural features of TRAP-positive structures and the similarity of the distribution patterns of TRAP and TSAP, it is suggested that TRAP co-exists with TSAP, and is located in lysosomes and secretory granules in osteoblasts.
Yamamoto
T
T
Department of Anatomy, Okayama University School of Dentistry, Japan. ytoshio@dent.okayama-u.ac.jp
Nagai
H
H
D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D007527:Isoenzymes / Q000032:analysis*; D008854:Microscopy, Electron; D015388:Organelles / Q000201:enzymology; D010006:Osteoblasts / Q000201:enzymology* / Q000648:ultrastructure*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase