title: Multicenter evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria.
abstract: We evaluated the BACTEC MGIT 960 system, which is a fully automated, noninvasive system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 7-ml culture tubes. We studied 3,330 specimens, 2,210 respiratory and 1,120 nonrespiratory specimens, collected from 2,346 patients treated at six sites. Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen slants and Middlebrook 7H11/7H11 selective plates. From all culture systems, a total of 362 isolates of mycobacteria were recovered; these were recovered from 353 specimens collected from 247 patients. The greatest number of isolates of mycobacteria (289, or 80% of the 362 isolates) was recovered with the BACTEC MGIT 960, followed by the BACTEC 460 TB (271, or 75%) and solid media (250, or 69%). From all culture systems a total of 132 isolates of Mycobacterium tuberculosis complex were recovered. The greatest number of isolates of M. tuberculosis complex was recovered when liquid medium was combined with conventional solid media; the number recovered with BACTEC 460 TB plus solid media was 128 (97%), that recovered with BACTEC MGIT 960 plus solid media was 121 (92%), that recovered with BACTEC 460 TB was 119 (90%) and that recovered with all solid media combined was 105 (79%). The recovery with BACTEC MGIT 960 alone was 102 (77%). The mean times to detection (TTD) for M. tuberculosis complex were 14.4 days for BACTEC MGIT, 15.2 days for BACTEC 460 TB, and 24.1 days for solid media. The numbers of isolates of Mycobacterium avium complex (MAC) recovered were 172 (100%) for all systems, 147 (85%) for BACTEC MGIT 960, 123 (72%) for BACTEC 460 TB, and 106 (62%) for all solid media combined. The TTD for MAC in each system were 10.0 days for BACTEC MGIT 960, 10.4 days for BACTEC 460 TB, and 25.9 days for solid media. Breakthrough contamination rates (percentages of isolates) for each of the systems were 8.1% for BACTEC MGIT 960, 4.9% for BACTEC 460 TB, and 21.1% for all solid media combined.
authors.affiliation: New York University School of Medicine, Bellevue Hospital, New York, New York, USA. bah2@is2.nyu.edu
authors.forename: B A
authors.initials: BA
authors.lastname: Hanna
authors.forename: A
authors.initials: A
authors.lastname: Ebrahimzadeh
authors.forename: L B
authors.initials: LB
authors.lastname: Elliott
authors.forename: M A
authors.initials: MA
authors.lastname: Morgan
authors.forename: S M
authors.initials: SM
authors.lastname: Novak
authors.forename: S
authors.initials: S
authors.lastname: Rusch-Gerdes
authors.forename: M
authors.initials: M
authors.lastname: Acio
authors.forename: D F
authors.initials: DF
authors.lastname: Dunbar
authors.forename: T M
authors.initials: TM
authors.lastname: Holmes
authors.forename: C H
authors.initials: CH
authors.lastname: Rexer
authors.forename: C
authors.initials: C
authors.lastname: Savthyakumar
authors.forename: A M
authors.initials: AM
authors.lastname: Vannier
mesh_terms: D001331:Automation; D001431:Bacteriological Techniques / Q000295:instrumentation*; D003470:Culture Media; D006801:Humans; D015269:Mycobacterium avium Complex / Q000254:growth & development / Q000302:isolation & purification*; D015270:Mycobacterium avium-intracellulare Infection / Q000175:diagnosis*; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D014376:Tuberculosis / Q000175:diagnosis*