title: Cytochrome P450 monooxygenase system in echinoderms.
abstract: The results of a limited number of studies on echinoderms provide evidence for the presence of a cytochrome P450 monooxygenase system in representatives of three classes of the phylum Echinodermata: the asteroids (sea stars), holothuroids (sea cucumbers) and echinoids (sea urchins). The monooxygenase system has been demonstrated to be involved in the metabolism of xenobiotic compounds, but is assumed to have its primary function in the metabolism of endogenous substrates, such as steroids. Available data on P450 cofactor requirement, P450-dependent metabolism of benzo[a]pyrene, studies with classical inhibitors of P450, specificity of P450 induction by planar compounds, and the changes in the benzo[a]pyrene metabolite profile in induced animals suggest similarities with the MO system present in vertebrates. However, the relatively high capacity of the monooxygenase system in sea stars to catalyse reactions with organic hydroperoxide as donor for activated oxygen, and the low induceability during exposure to xenobiotics indicate also important differences between the echinoderm cytochrome P450 monooxygenase system and that of vertebrates. Some evidence was found for the existence of different forms of cytochrome P450 in sea stars. Catalytic functions of the cytochrome P450 monooxygenase system of sea stars in the metabolism of steroids may be suppressed as a result of the induction of cytochrome P450 by xenobiotics.
authors.affiliation: AquaSense Laboratory, Amsterdam, Netherlands. p.dbesten@riza.rws.minvenw.nl
authors.forename: P J
authors.initials: PJ
authors.lastname: den Besten
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000378:metabolism; D015153:Blotting, Western; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D015786:Cytochromes b5 / Q000737:chemistry; D004447:Echinodermata / Q000201:enzymology* / Q000502:physiology; D004790:Enzyme Induction; D005260:Female; D008297:Male; D008861:Microsomes / Q000201:enzymology; D012098:Reproduction; D012621:Seasons; D012737:Sex Factors; D013057:Spectrum Analysis