Identification of contaminating fungal DNA sequences in Zymolyase.
When different preparations of Zymolyase were included in the pretreatment protocol of a panfungal PCR assay using a primer system for the 18S rRNA gene, an amplification product occurred in negative controls. The amplified fragment showed 100.0% sequence identity to the Saccharomyces sensu stricto complex and Kluyveromyces lodderae. Lyticase, lysing enzymes, and proteinase K appeared to be free from fungal DNA.
Rimek
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Hygiene Institute, Children's Hospital, University of Heidelberg, Heidelberg, Germany. dagmar_rimek@ukl.uniheidelberg.de
Garg
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Haas
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Kappe
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D017931:DNA Primers; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D004340:Drug Contamination; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D007716:Kluyveromyces / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012440:Saccharomyces / Q000235:genetics / Q000302:isolation & purification*