title: Leishmania major: histone H1 gene expression from the sw3 locus.
abstract: Histone H1 in the parasitic protozoan Leishmania is a developmentally regulated protein encoded by the sw3 gene. Here we report that histone H1 variants exist in different Leishmania species and strains of L. major and that they are encoded by polymorphic genes. Amplification of the sw3 gene from the genome of three strains of L. major gave rise to different products in each strain, suggesting the presence of a multicopy gene family. In L. major, these genes were all restricted to a 50-kb Bg/II fragment found on a chromosomal band of 1.3 Mb (chromosome 27). The detection of RFLPs in this locus demonstrated its heterogeneity within several species and strains of Leishmania. Two different copies of sw3 (sw3.0 and sw3.1) were identified after screening a cosmid library containing L. major strain Friedlin genomic DNA. They were identical in their 5' UTRs and open reading frames, but differed in their 3' UTRs. With respect to the originally cloned copy of sw3 from L. major strain LV39, their open reading frames lacked a repeat unit of 9 amino acids. Immunoblots of L. guyanensis parasites transfected with these cosmids revealed that both copies could give rise to the histone H1 protein. The characterization of this locus will now make possible a detailed analysis of the function of histone H1 in Leishmania, as well as permit the dissection of the molecular mechanisms governing the developmental regulation of the sw3 gene.
authors.affiliation: Biochemistry Institute, University of Lausanne, Switzerland.
authors.forename: S
authors.initials: S
authors.lastname: Belli
authors.forename: A
authors.initials: A
authors.lastname: Formenton
authors.forename: T
authors.initials: T
authors.lastname: Noll
authors.forename: A
authors.initials: A
authors.lastname: Ivens
authors.forename: R
authors.initials: R
authors.lastname: Jacquet
authors.forename: C
authors.initials: C
authors.lastname: Desponds
authors.forename: D
authors.initials: D
authors.lastname: Hofer
authors.forename: N
authors.initials: N
authors.lastname: Fasel
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015139:Blotting, Southern; D015153:Blotting, Western; D003001:Cloning, Molecular; D017931:DNA Primers; D016054:DNA, Protozoan / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D004591:Electrophoresis, Polyacrylamide Gel; D018507:Gene Expression Regulation, Developmental; D015723:Gene Library; D006657:Histones / Q000737:chemistry / Q000235:genetics*; D018320:Leishmania major / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011817:Rabbits; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid