Effect of increased afterload on cardiac lipoprotein lipase and VLDL receptor expression.
Fatty acids are a major source of fuel for energy production by myocytes. Lipoprotein lipase (LPL) and very low density lipoprotein (VLDL) receptor are abundantly expressed by the heart and skeletal muscles. LPL and possibly VLDL receptor represent the primary route of access to fatty acids contained in circulating triglyceride-rich lipoproteins. Physical exercise and thyroid hormone, which promote energy consumption, upregulate LPL expression in skeletal muscles. This study tested the hypothesis that increased cardiac workload might modulate myocardial LPL and/or VLDL receptor expressions. Accordingly, cardiac tissue LPL activity, LPL and VLDL receptor proteins and mRNA abundance were studied in Sprague-Dawley rats 4 weeks after induction of severe thoracic aorta constriction or sham operation. Elevation of afterload with thoracic aortic constriction led to a significant cardiomegaly and a marked upregulation of cardiac LPL activity, LPL mRNA and LPL protein abundance, but did not modify VLDL receptor mRNA or protein abundance. Thus, increased cardiac workload in this model results in upregulation of myocardial LPL expression which can enhance fatty acid availability to accommodate the heart's increased energy requirement.
Vaziri
N D
ND
Department of Medicine, University of California, Irvine 92697, USA. tabotten@uci.edu
Liang
K
K
Barton
C H
CH
D000818:Animals; D001013:Aorta, Thoracic; D001017:Aortic Coarctation / Q000150:complications / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D017379:Hypertrophy, Left Ventricular / Q000209:etiology / Q000235:genetics* / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics* / Q000378:metabolism*; D015854:Up-Regulation