title: Recombinant human monoclonal antibodies to Ebola virus.
abstract: Human Fab (IgG1kappa) phage display libraries were constructed from bone marrow RNA from 2 donors who recovered from infection with Ebola (EBO) virus during the 1995 outbreak in Kikwit, Democratic Republic of the Congo. The libraries were initially panned against a radiation-inactivated EBO virus-infected Vero cell lysate, but only weak binders were identified. In contrast, panning against secreted EBO glycoprotein (SGP) resulted in Fabs showing very strong reactivity with SGP in ELISA. These Fabs also reacted with a virion membrane preparation. The Fabs were strongly positive in IFAs with cells infected with EBO (subtype Zaire) virus but negative with uninfected cells, with a characteristic punctate staining pattern in the cytoplasm. The Fabs showed weak or no reactivity with the virus cell lysate although donor serum did react. The Fabs are now being characterized in structural and functional terms. Major interest will focus on the ability of antibodies to neutralize EBO virus and, later, to protect animals against infection.
authors.affiliation: Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
authors.forename: T
authors.initials: T
authors.lastname: Maruyama
authors.forename: P W
authors.initials: PW
authors.lastname: Parren
authors.forename: A
authors.initials: A
authors.lastname: Sanchez
authors.forename: I
authors.initials: I
authors.lastname: Rensink
authors.forename: L L
authors.initials: LL
authors.lastname: Rodriguez
authors.forename: A S
authors.initials: AS
authors.lastname: Khan
authors.forename: C J
authors.initials: CJ
authors.lastname: Peters
authors.forename: D R
authors.initials: DR
authors.lastname: Burton
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002522:Chlorocebus aethiops; D015023:Democratic Republic of the Congo; D029043:Ebolavirus / Q000145:classification / Q000276:immunology*; D019142:Hemorrhagic Fever, Ebola / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D007145:Immunoglobulin kappa-Chains / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D019151:Peptide Library; D014709:Vero Cells