Identification of seven Treponema species in health- and disease-associated dental plaque by nested PCR.
Species-specific nested PCR was used to detect Treponema amylovorum, Treponema denticola, Treponema maltophilum, Treponema medium, Treponema pectinovorum, Treponema socranskii, and Treponema vincentii in dental plaque. Subjects with periodontitis harbored all species, but T. pectinovorum and T. vincentii were not found in plaque from disease-free subjects.
Willis
S G
SG
Departments of Pediatric Dentistry, Oregon Health Sciences University, Portland, Oregon 97201-3097, USA.
Smith
K S
KS
Dunn
V L
VL
Gapter
L A
LA
Riviere
K H
KH
Riviere
G R
GR
D017931:DNA Primers; D003773:Dental Plaque / Q000821:virology*; D006801:Humans; D010510:Periodontal Diseases / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012016:Reference Values; D014208:Treponema / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification