title: Characterisation of dermonecrotic toxin-producing strains of Pasteurella multocida subsp. multocida isolated from man and swine.
abstract: Thirty-six isolates, from man or swine, of Pasteurella multocida subsp. multocida producing (n = 13) or not producing (n = 23) the dermonecrotic toxin (DNT) were studied by numerical analysis, capsular typing and ribotyping. Toxigenic strains were also characterised by restriction fragment length polymorphism (RFLP) of the toxA gene and pulsed-field gel electrophoresis (PFGE). Numerical analysis differentiated the Pasteurella species and subspecies, but did not discriminate between toxigenic and nontoxigenic strains. RFLP demonstrated that toxA was located in a conserved part of the chromosome of all toxigenic strains. Ribotyping provided evidence of a close association between DNT production and one of the six EcoRI ribotypes designated as E2. In contrast, PFGE provided evidence for significant DNA polymorphism amongst the toxigenic strains. Results of phenotypic and genotypic studies suggested that toxigenic strains do not form a clone within the subspecies multocida. No difference was found between toxigenic strains of porcine or human origin by biochemical characterisation, capsular serotyping or genomic typing methods.
authors.forename: P-Y
authors.initials: PY
authors.lastname: Donnio
authors.affiliation: INSERM U341, Faculté de Médecine, 30000 Nimes.
authors.forename: A
authors.initials: A
authors.lastname: Allardet-Servent
authors.forename: M
authors.initials: M
authors.lastname: Perrin
authors.affiliation: Laboratoire des Pasteurella, Institut Pasteur, 75724 Paris, France.
authors.forename: F
authors.initials: F
authors.lastname: Escande
authors.forename: J-L
authors.initials: JL
authors.lastname: Avril
mesh_terms: D000818:Animals; D016667:Bacterial Capsules / Q000032:analysis; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D004275:DNA, Ribosomal / Q000032:analysis / Q000737:chemistry; D003885:Dermotoxins / Q000096:biosynthesis* / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D004797:Enzyme-Linked Immunosorbent Assay; D005838:Genotype; D006801:Humans; D010326:Pasteurella Infections / Q000382:microbiology* / Q000635:transmission; D016979:Pasteurella multocida / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012424:Rural Population; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000635:transmission