cDNA sequence and overexpression of chloroplast chaperonin 21 from Arabidopsis thaliana.
Higher plant chloroplasts contain a 21-kDa protein, chaperonin 21 (Cpn21), that is a functional homolog of the chaperonin 10 (Cpn10). The chloroplast Cpn21 polypeptide consists of two Cpn10-like domains fused together in tandem. We describe here the cDNA sequence of the Cpn21 (AtCpn21) precursor protein from Arabidopsis thaliana. The deduced amino acid sequence of the AtCpn21 precursor protein, 253 amino acids long, shows 61% identity with the spinach Cpn21 protein. The AtCpn21 precursor protein contains the typical chloroplast transit peptide of 51 amino acids at its aminoterminus and the two Cpn10-like domains which exhibits 46% sequence identity to each other. The predicted mature-sized polypeptide of AtCpn21 was expressed in Escherichia coli as a soluble 21-kDa protein. Gel-filtration and chemical cross-linking analyses showed that the recombinant mature AtCpn21 protein forms a stable homo-oligomer composed of three or four polypeptides.
Hirohashi
T
T
Institute for Protein Research, Osaka University, Japan.
Nishio
K
K
Nakai
M
M
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D018833:Chaperonins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D002736:Chloroplasts / Q000378:metabolism; D018076:DNA, Complementary / Q000737:chemistry*; D004926:Escherichia coli / Q000378:metabolism; D015870:Gene Expression; D056384:Group I Chaperonins; D008969:Molecular Sequence Data; D011498:Protein Precursors / Q000235:genetics*; D016415:Sequence Alignment