title: Output of liver fatty acid-binding protein (L-FABP) in bile.
abstract: Liver fatty acid-binding protein (L-FABP) is a small cytoplasmic molecule highly expressed in the liver. Since L-FABP exhibits affinities for several biliary components, its presence in bile was explored by Western blotting and competitive ELISA in various mammalian species. A L-FABP-like immunoreactivity was consistently found in both hepatic and gallbladder bile. A close molecular identity between this 14 kDa biliary protein and the purified L-FABP was assessed by immunological analyses and high performance capillary electrophoresis. Pharmacological induction of hepatic L-FABP biosynthesis led to a similar increase in biliary L-FABP levels showing a close relationships between the cytosolic and biliary contents of this protein. Finally, a correlation between the presence of L-FABP in bile and both bile flow and bile acid release was found. These data suggest an output of L-FABP in bile in normal conditions which might be coupled with the physiological release of biliary components.
authors.affiliation: Laboratoire de Physiologie de la Nutrition, Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation (ENSBANA), Dijon, France.
authors.forename: L
authors.initials: L
authors.lastname: Foucaud
authors.forename: J
authors.initials: J
authors.lastname: Grillasca
authors.forename: I
authors.initials: I
authors.lastname: Niot
authors.forename: N
authors.initials: N
authors.lastname: Domingo
authors.forename: H
authors.initials: H
authors.lastname: Lafont
authors.forename: R
authors.initials: R
authors.lastname: Planells
authors.forename: P
authors.initials: P
authors.lastname: Besnard
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism*; D002352:Carrier Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids / Q000378:metabolism*; D005704:Gallbladder / Q000378:metabolism; D006801:Humans; D007120:Immunochemistry; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008970:Molecular Weight; D018996:Myelin P2 Protein / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D051381:Rats; D017208:Rats, Wistar; D025521:Tumor Suppressor Proteins