Influence of sperm plasma membrane destruction on human sperm head decondensation and pronuclear formation.
This study investigated the ability of membrane-damaged sperm to undergo sperm head decondensation and male pronuclear forrmation following intracytoplasmic sperm injection. For induction of membrane-damage, sperm were exposed to 0.1% Triton X-100 for a few seconds. The single sperm curling (SSC) test was performed to evaluate the viability of the sperm. Of the injected oocytes 49 and 47% developed male pronuclei in the experimental and control groups, respectively. Sperm head decondensation was much higher in the membrane-damaged group (84 versus 70%). This study clearly shows that ICSI of membrane-damaged sperm does not affect pronuclear formation, although sperm head decondensation is much higher.
Ahmadi
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Department of Obstetrics and Gynaecology, National University of Singapore, Singapore.
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D000818:Animals; D002462:Cell Membrane / Q000502:physiology*; D002467:Cell Nucleus / Q000502:physiology*; D002470:Cell Survival; D006224:Cricetinae; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D008297:Male; D008845:Microinjections; D013077:Sperm Head / Q000648:ultrastructure*; D013094:Spermatozoa / Q000502:physiology / Q000648:ultrastructure*