Characteristics of gene 28 product, the constituent of the central part of bacteriophage T4 baseplate.
The phage TD gene 28 product has been partially characterized and its biological role has been examined. It was found to be a protein with a molecular size of 24 kDa which cosediments with the membrane fraction of the bacterial extracts and could only be washed out by a 0.2% sarcosyl solution. Other observations indicate that gp 28 has a majority of hydrophobic residues on its surface and forms a homotrimeric complex in the absence of other phage proteins. The product was finally identified as a baseplate structural component. Incubation of purified phage preparation in a buffer which contained active protein 28, did not affect the efficiency of the plating. However, incubation of the phage particles with specific antiserum was found to neutralize phage infectivity.
Nieradko
J
J
Department of Microbiology, University of Gdańsk, Poland. nieradko@biotech.univ.gda.pl
Koszałka
P
P
Krzywicka
A
A
D017122:Bacteriophage T4 / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000821:virology*; D005814:Genes, Viral; D005816:Genetic Complementation Test; D015151:Immunoblotting; D009500:Neutralization Tests; D015678:Viral Structural Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D019065:Virus Assembly