title: vig-1, a new fish gene induced by the rhabdovirus glycoprotein, has a virus-induced homologue in humans and shares conserved motifs with the MoaA family.
abstract: We used mRNA differential display methodology to analyze the shift of transcription profile induced by the fish rhabdovirus, viral hemorrhagic septicemia virus (VHSV), in rainbow trout leukocytes. We identified and characterized a new gene which is directly induced by VHSV. This VHSV-induced gene (vig-1) encodes a 348-amino-acid protein. vig-1 is highly expressed during the experimental disease in lymphoid organs of the infected fish. Intramuscular injection of a plasmid vector expressing the viral glycoprotein results in vig-1 expression, showing that the external virus protein is sufficient for the induction. vig-1 expression is also obtained by a rainbow trout interferon-like factor, indicating that vig-1 can be induced through different pathways. Moreover, vig-1 is homologous to a recently described human cytomegalovirus-induced gene. Accordingly, vig-1 activation may represent a new virus-induced activation pathway highly conserved in vertebrates. The deduced amino acid sequence of vig-1 is significantly related to sequences required for the biosynthesis of metal cofactors. This suggests that the function of vig-1 may be involved in the nonspecific virus-induced synthesis of enzymatic cofactors of the nitric oxide pathway.
authors.affiliation: Institut National de la Recherche Agronomique, Unité de Virologie et Immunologie Moléculaires, 78352 Jouy-en-Josas cedex, France.
authors.forename: P
authors.initials: P
authors.lastname: Boudinot
authors.forename: P
authors.initials: P
authors.lastname: Massin
authors.forename: M
authors.initials: M
authors.lastname: Blanco
authors.forename: S
authors.initials: S
authors.lastname: Riffault
authors.forename: A
authors.initials: A
authors.lastname: Benmansour
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D005393:Fish Diseases / Q000235:genetics*; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000502:physiology*; D006801:Humans; D008969:Molecular Sequence Data; D009569:Nitric Oxide / Q000502:physiology; D017686:Oncorhynchus mykiss / Q000235:genetics*; D012209:Rhabdoviridae / Q000502:physiology*; D014764:Viral Proteins / Q000502:physiology*