title: Proline isomerization is unlikely to be the cause of slow annealing and reactivation during the folding of alkaline phosphatase.
abstract: The in vitro folding of Escherichia coli alkaline phosphatase (AP) from the guanidine hydrochloride (GdnHCl) denatured state is characterized by a significant slow phase in the post activational recovery of native protein lability (probed by the susceptibility to GdnHCl denaturation and occurring on the time scale of days) as well as a slow phase in the recovery of activity (on the time scale of minutes). Slow folding events have often been attributed to cis-trans isomerizations of X-Pro peptide bonds, a plausible explanation for AP, which contains 21 prolines per subunit. To investigate the role of proline isomerization in the two measures of refolding mentioned above, we have performed "double-jump" GdnHCl denaturation/renaturation experiments, with a third jump, where the rate of unfolding of refolded protein upon exposure to denaturant was added to assess the rate of change of lability. Our measurements of the time evolution of both the lability and the reactivation of refolded AP as a function of denaturation time show that proline isomerization is unlikely to be the cause of either of these slow events in the refolding of AP. The conclusions are further confirmed by the absence of proline isomerization effects when AP is refolded in the presence of human and periplasmic E. coli peptidyl-prolyl isomerase.
authors.affiliation: Biophysics Research Division, University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: E
authors.initials: E
authors.lastname: Dirnbach
authors.forename: D G
authors.initials: DG
authors.lastname: Steel
authors.forename: A
authors.initials: A
authors.lastname: Gafni
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D004789:Enzyme Activation; D006801:Humans; D007536:Isomerism; D007700:Kinetics; D011392:Proline / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D013379:Substrate Specificity