Genomic fingerprinting of Proteus species using repetitive sequence based PCR (rep-PCR).
Three Proteus species P. vulgaris, P. mirabilis and P. penneri have been characterized by repetitive sequence-based PCR. Four families of repetitive sequence based primers REP, ERIC, BOXA1R and BOXA2R, give specific patterns for each Proteus species. Species differentiation was best afforded using BOXA2R for detection of P. mirabilis, either REP-Dt or BOXA1R primers for detection of P. penneri and ERIC primer pair for P. vulgaris.
Serwecińska
L
L
Institute of Microbiology and Immunology, University of Lódź, Poland.
Cieślikowski
T
T
Pytlos
M
M
Jaworski
A
A
Kaca
W
W
D015373:Bacterial Typing Techniques; D016172:DNA Fingerprinting; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis; D016133:Polymerase Chain Reaction / Q000379:methods*; D011511:Proteus / Q000145:classification* / Q000235:genetics / Q000254:growth & development; D012091:Repetitive Sequences, Nucleic Acid; D013045:Species Specificity