title: Spread of amikacin resistance in Acinetobacter baumannii strains isolated in Spain due to an epidemic strain.
abstract: Sixteen amikacin-resistant clinical Acinetobacter baumannii isolates from nine different hospitals in Spain were investigated to determine whether the high incidence of amikacin-resistant A. baumannii was due to the dissemination of an amikacin-resistant strain or to the spread of an amikacin resistance gene. The epidemiological relationship studied by repetitive extragenic palindromic PCR and low-frequency restriction analysis of chromosomal DNA showed that the same clone was isolated in eight of nine hospitals, although other clones were also found. The strains were studied for the presence of the aph(3')-VIa and aac(6')-I genes, which encode enzymes which inactivate amikacin, by PCR. All 16 clinical isolates had positive PCRs with primers specific for the amplification of the aph(3')-VIa gene, whereas none had a positive reaction for the amplification of the aac(6')-I gene. Therefore, the high incidence of amikacin resistance among clinical A. baumannii isolates in Spain was mainly due to an epidemic strain, although the spread of the aph(3')-VI gene cannot be ruled out.
authors.affiliation: Department de Microbiologia, Institut d' Investigació Biomèdica August Pi i Sunyer, Hospital Clínic, Facultat de Medicina, Universitat de Barcelona, 08036 Barcelona, Spain. vila@medicina.ub.es
authors.forename: J
authors.initials: J
authors.lastname: Vila
authors.forename: J
authors.initials: J
authors.lastname: Ruiz
authors.forename: M
authors.initials: M
authors.lastname: Navia
authors.forename: B
authors.initials: B
authors.lastname: Becerril
authors.forename: I
authors.initials: I
authors.lastname: Garcia
authors.forename: S
authors.initials: S
authors.lastname: Perea
authors.forename: I
authors.initials: I
authors.lastname: Lopez-Hernandez
authors.forename: I
authors.initials: I
authors.lastname: Alamo
authors.forename: F
authors.initials: F
authors.lastname: Ballester
authors.forename: A M
authors.initials: AM
authors.lastname: Planes
authors.forename: J
authors.initials: J
authors.lastname: Martinez-Beltran
authors.forename: T J
authors.initials: TJ
authors.lastname: de Anta
mesh_terms: D000150:Acinetobacter / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D000151:Acinetobacter Infections / Q000453:epidemiology* / Q000382:microbiology; D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002876:Chromosomes, Bacterial / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004352:Drug Resistance, Microbial; D006801:Humans; D016133:Polymerase Chain Reaction; D013030:Spain / Q000453:epidemiology