Use of restriction fragment analysis and sequencing of a serotype-specific region to type adenovirus isolates.
Restriction fragment analysis and sequencing of a serotype-specific region were used to type 12 and 2 clinical isolates, respectively. Both molecular methods produced clear-cut results that completely correlated with that of the neutralization test.
Li
Q G
QG
Department of Virology, Umeâ University, Umeâ, Sweden.
Henningsson
A
A
Juto
P
P
Elgh
F
F
Wadell
G
G
D000257:Adenoviridae Infections / Q000175:diagnosis*; D000260:Adenoviruses, Human / Q000235:genetics / Q000302:isolation & purification*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D002675:Child, Preschool; D003231:Conjunctivitis / Q000821:virology; D017931:DNA Primers; D003967:Diarrhea / Q000821:virology; D005243:Feces / Q000821:virology; D006801:Humans; D007223:Infant; D008590:Meningoencephalitis / Q000821:virology; D008969:Molecular Sequence Data; D010612:Pharyngitis / Q000821:virology; D011024:Pneumonia, Viral / Q000821:virology; D015183:Restriction Mapping / Q000379:methods; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012703:Serotyping / Q000379:methods