title: Stimulation of immunoreactive insulin release by glucose in rat brain synaptosomes.
abstract: The effect of glucose on the release of immunoreactive insulin (IRI) in synaptosomes isolated from rat brain was studied. In the absence of glucose synaptosomes release about 4% (0.77 microIU/mg protein) of total content. Glucose increases significantly the IRI released by synaptosomes. Addition of the glycolytic inhibitor iodoacetic acid (IAA), decreased the glucose-induced release of IRI by about 50%, suggesting that glucose metabolism is involved. The observation that glucose provides a concentration related signal for IRI release indicates that this synaptosomal preparation may be useful as a model for research on the mechanism of insulin release in brain.
authors.affiliation: Center for Neurosciences of Coimbra, Department of Zoology, University of Coimbra, Portugal.
authors.forename: M S
authors.initials: MS
authors.lastname: Santos
authors.forename: E M
authors.initials: EM
authors.lastname: Pereira
authors.forename: A P
authors.initials: AP
authors.lastname: Carvaho
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D006019:Glycolysis / Q000187:drug effects; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D019807:Iodoacetic Acid / Q000494:pharmacology; D007700:Kinetics; D008297:Male; D009840:Oligomycins / Q000494:pharmacology; D011190:Potassium Cyanide / Q000494:pharmacology; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*