Detection of mutations by automated fluorescence/RNA-based dideoxy fingerprinting (ARddF)
Dideoxy fingerprinting (ddF) is a hybrid technique which combines aspects of single strand conformational polymorphism (SSCP) and dideoxy sequencing to detect the presence of single base changes in a defined fragment of nucleic acid. ddF is no more technically demanding than SSCP, yet it is more sensitive in detecting point mutations. We describe here the adaptation of conventional ddF to an automated sequencing system using fluorescent Cy5 labeled primers. We show that automated RNA-based ddF (ARddF) has several advantages over conventional radioisotope-based ddF, including: (1) analysis of larger nucleic acid fragments (up to 10(3) bp), due to the ability to continuously analyse and compile sequencing information; (2) greater reliability for distinguishing mutant sequences from wild type sequences (particularly when the mutation leads to gain or loss of a dideoxy termination segment); (3) the use of fluorescent labeled primers, making ARddF less hazardous than methods requiring radionucleotides. The use of ARddF in conjunction with new methods for isolating RNA from a [corrected] small number of cells facilitates mutational analysis of small tissue biopsies and other limited samples, and will allow more widespread application of mutational screening in the setting of clinical diagnostic laboratories.
Martincic
D
D
Department of Pediatrics, Vanderbilt University School of Medicine and Veterans Administration Medical Center, Nashville, Tennessee 37232-6310, USA.
Koury
M J
MJ
Gale
K
K
Whitlock
J A
JA
D000818:Animals; D001331:Automation; D005453:Fluorescence; D016158:Genes, p53; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods*; D017354:Point Mutation; D018807:Polymorphism, Single-Stranded Conformational; D012313:RNA / Q000032:analysis*; D014407:Tumor Cells, Cultured