title: Western blot banding pattern in early Lyme borreliosis among patients from an endemic region of north-eastern Poland.
abstract: Aim of this study was evaluation of Western blot banding patterns in different clinical forms of early Lyme borreliosis diagnosed in patients from north-eastern Poland, recognized as endemic for tick-borne diseases. Study was performed on serum samples of 48 patients with Lyme borreliosis and 26 healthy volunteers, as controls. Samples tested routinely for total antibody with enzyme immunoassay were subsequently analysed for specific antibodies with Western blot based on antigen extract of European strain of Borrelia burgdorferi. In patients, IgM antibodies were the most frequently directed against 41 kDa and 58 kDa antigens, whereas in control group only antibodies against 45 kDa and 58 kDa were present. Similar response was observed in respect to IgG antibodies. Evaluation of banding pattern in respect to clinical form of the disease revealed the highest prevalence of IgM and IgG anti-41 kDa antibodies in patients with erythema migrans and Lyme arthritis, and anti-58 kDa in neuroborreliosis patients, who had no anti-21 kDa antibodies. Relatively high frequency of IgG antibodies against 21, 30 and 93 kDa antigens was typical for neuroborreliosis. Bands count was significantly higher in different clinical forms of the disease than in controls, and it was the highest in neuroborreliosis. Combined analysis of Western blot results (IgM/IgG) enabled to achieve higher sensitivity (84%) and specificity (100%) than available with the most recommended EIA kits.
authors.affiliation: Department of Infectious Diseases, Medical Academy of Białystok.
authors.forename: R
authors.initials: R
authors.lastname: Flisiak
authors.forename: I
authors.initials: I
authors.lastname: Wierzbicka
authors.forename: D
authors.initials: D
authors.lastname: Prokopowicz
mesh_terms: D000208:Acute Disease; D000907:Antibodies, Bacterial / Q000032:analysis; D015153:Blotting, Western; D015748:Borrelia burgdorferi Group / Q000302:isolation & purification*; D019353:Endemic Diseases; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D016014:Linear Models; D008193:Lyme Disease / Q000097:blood / Q000175:diagnosis* / Q000453:epidemiology*; D008297:Male; D011044:Poland / Q000453:epidemiology; D012680:Sensitivity and Specificity; D016036:Seroepidemiologic Studies