title: Theoretical and experimental tests of a chromosomal fingerprint for densely ionizing radiation based on F ratios calculated from stable and unstable chromosome aberrations.
abstract: In the present study, F ratios for both stable chromosome aberrations, i.e. ratios of translocations to pericentric inversions, and unstable aberrations, i.e. dicentrics and centric rings, were measured using fluorescence in situ hybridization. F ratios for stable aberrations measured after exposure to low (2.89 Gy 60Co gamma rays) and high-LET (0.25 Gy 56Fe ions; 1.25 Gy 56Fe ions; 3.0 Gy 12C ions) radiation were 6.5 +/- 1.5, 4.7 +/- 1.6, 9.3 +/- 2.5 and 10.4 +/- 3.0, respectively. F ratios for unstable aberrations measured after low (2.89 Gy 60Co gamma rays) and high-LET (0.25 Gy 56Fe ions; 3.0 Gy 12C ions) radiations were 6.5 +/- 1.6, 6.3 +/- 2.3 and 11.1 +/- 3.7, respectively. No significant difference between the F ratios for low- and high-LET radiation was found. Further tests on the models for calculation of the F ratio proposed by Brenner and Sachs (Radiat. Res. 140, 134-142, 1994) showed that the F ratio may not be straightforward as a practical fingerprint for densely ionizing radiation.
authors.affiliation: University of California, Lawrence Livermore National Laboratory, Livermore 94550, USA.
authors.forename: J N
authors.initials: JN
authors.lastname: Lucas
authors.forename: W
authors.initials: W
authors.lastname: Deng
authors.forename: S W
authors.initials: SW
authors.lastname: Oram
authors.forename: F S
authors.initials: FS
authors.lastname: Hill
authors.forename: M
authors.initials: M
authors.lastname: Durante
authors.forename: K
authors.initials: K
authors.lastname: George
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: C L
authors.initials: CL
authors.lastname: Owens
authors.forename: T
authors.initials: T
authors.lastname: Yang
mesh_terms: D002478:Cells, Cultured; D007446:Chromosome Inversion; D002877:Chromosomes, Human / Q000528:radiation effects*; D016172:DNA Fingerprinting; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018499:Linear Energy Transfer; D008214:Lymphocytes / Q000528:radiation effects / Q000648:ultrastructure; D014178:Translocation, Genetic