Markers for neuronal degeneration in organotypic slice cultures.
This protocol describes ways of monitoring spontaneous or induced neuronal degeneration in organotypic brain slice cultures. Hippocampal cultures (4-week-old) are grown in normal serum-free control medium, or exposed to the neurotoxin trimethyltin (TMT) (0.5-100 microM) for 24 h or the excitotoxic glutamate agonist kainic acid (KA) (5-25 microM) for 48 h followed by 24 h or 48 h, respectively, in normal medium. Corticostriatal slice cultures (also 4-week-old) are exposed to KA (6-24 microM) for 48 h and normal medium for control. The resulting neurodegeneration is estimated by (a) propidium iodide (PI) uptake, (b) lactate dehydrogenase (LDH) efflux to the culture medium, (c) ordinary Nissl cell staining, (d) staining by the neurodegenerative marker Fluoro-Jade (FJ), (e) neuronal microtubule degeneration by immunohistochemical staining for microtubule-associated protein 2 (MAP2), and (f) Timm sulphide silver staining for heavy metal alterations. Both hippocampal and corticostriatal slice cultures show a dose- and time-dependent increase in PI uptake and LDH efflux after exposure to TMT and KA. The mean PI uptake and the LDH efflux into the medium correlate well for both types of cultures. Both TMT and KA exposed hippocampal cultures display in vivo patterns of differential neuronal vulnerability as evidenced by PI uptake, FJ staining and MAP2 immunostaining. Corticostriatal slice cultures exposed to a high dose of KA display extensive striatal and cortical degeneration in FJ staining as suggested by a high PI uptake. A change in Timm sulphide silver staining in deep central parts of some control cultures, corresponds to areas with loss of cells in cell staining, loss of MAP2 staining, PI uptake, and FJ staining. We conclude that organotypic brain slice cultures, in combination with appropriate markers in standardized protocols, represent feasible means for studies of excitotoxic and neurotoxic compounds.
Noraberg
J
J
Department of Anatomy and Cell Biology, University of Odense, Denmark. j.noraberg@winsloew.ou.dk
Kristensen
B W
BW
Zimmer
J
J
D000818:Animals; D000831:Animals, Newborn; D015415:Biomarkers; D015220:Calcium Channels / Q000378:metabolism; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000737:chemistry* / Q000187:drug effects / Q000473:pathology; D003035:Cobalt / Q000378:metabolism; D004396:Coloring Agents / Q000378:metabolism; D003342:Corpus Striatum / Q000737:chemistry* / Q000187:drug effects / Q000473:pathology; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity; D005456:Fluorescent Dyes / Q000378:metabolism; D006624:Hippocampus / Q000737:chemistry* / Q000187:drug effects / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D017136:Ion Transport; D007608:Kainic Acid / Q000633:toxicity; D008869:Microtubule-Associated Proteins / Q000032:analysis; D009410:Nerve Degeneration / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D009419:Nerve Tissue Proteins / Q000032:analysis; D009474:Neurons / Q000737:chemistry / Q000187:drug effects*; D011419:Propidium / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D016622:Silver Staining / Q000379:methods; D014048:Tolonium Chloride / Q000378:metabolism