title: galectin-1 and galectin-3 expression in human bladder transitional-cell carcinomas.
abstract: Galectin-1 and galectin-3 are galactoside-binding proteins involved in different steps of tumor progression and potential targets for therapy. We have investigated the expression of these galectins in 38 human bladder transitional-cell carcinomas of different histological grade and clinical stage and in 5 normal urothelium samples. Galectin-1 mRNA levels were highly increased in most high-grade tumors compared with normal bladder or low-grade tumors. Western blot and immuno-histochemical analysis of normal and neoplastic tissues revealed a higher content of galectin-1 in tumors. Galectin-3 mRNA levels were also increased in most tumors compared with normal urothelium, but levels were comparable among tumors of different histological grade.
authors.affiliation: Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Centro di Endocrinologia ed Oncologia Sperimentale G. Salvatore del Consiglio Nazionale delle Ricerche, Università degli Studi di Napoli Federico II, Naples, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Cindolo
authors.forename: G
authors.initials: G
authors.lastname: Benvenuto
authors.forename: P
authors.initials: P
authors.lastname: Salvatore
authors.forename: R
authors.initials: R
authors.lastname: Pero
authors.forename: G
authors.initials: G
authors.lastname: Salvatore
authors.forename: V
authors.initials: V
authors.lastname: Mirone
authors.forename: D
authors.initials: D
authors.lastname: Prezioso
authors.forename: V
authors.initials: V
authors.lastname: Altieri
authors.forename: C B
authors.initials: CB
authors.lastname: Bruni
authors.forename: L
authors.initials: L
authors.lastname: Chiariotti
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000943:Antigens, Differentiation / Q000096:biosynthesis* / Q000235:genetics; D015152:Blotting, Northern; D015153:Blotting, Western; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D037483:Galectin 1; D037502:Galectin 3; D006388:Hemagglutinins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D014521:Urethra / Q000378:metabolism; D001743:Urinary Bladder / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology