The lethal effect of a benzamide derivative, 3-methoxybenzamide, can be suppressed by mutations within a cell division gene, ftsZ, in Bacillus subtilis.
3-Methoxybenzamide (3-MBA), which is known to be an inhibitor of ADP-ribosyltransferase, inhibits cell division in Bacillus subtilis, leading to filamentation and eventually lysis of cells. Our genetic analysis of 3-MBA-resistant mutants indicated that the primary target of the drug is the cell division system involving FtsZ function during both vegetative growth and sporulation.
Ohashi
Y
Y
Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo 171-8501, Japan.
Chijiiwa
Y
Y
Suzuki
K
K
Takahashi
K
K
Nanamiya
H
H
Sato
T
T
Hosoya
Y
Y
Ochi
K
K
Kawamura
F
F
D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D001549:Benzamides / Q000633:toxicity*; D002455:Cell Division / Q000187:drug effects; D003598:Cytoskeletal Proteins; D004352:Drug Resistance, Microbial / Q000235:genetics; D009154:Mutation; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000187:drug effects; D013489:Suppression, Genetic; D014157:Transcription Factors