Immunodetection of lymphocyte subpopulations involved in allograft rejection in a teleost, Dicentrarchus labrax (L.).
Monoclonal antibodies which recognize antigenic determinants expressed by T-cells and Ig-bearing cells, respectively, allowed lymphocyte subpopulations involved in allograft rejection of muscle transplants to be identified in the teleost fish Dicentrarchus labrax (L.). The monoclonal antibody DLT15 first allowed recognizing T-cells involved in an in vivo antigen-driven cellular response in teleosts. Immunohistochemical studies showed a high density of lymphocytes in allografts and provided evidence of predominance of T-cells. The heterogeneity of the cell populations recognized by the antibodies was evidenced by the different size, cytology, and staining patterns of T-cells and Ig-bearing cells.
Abelli
L
L
Dipartimento di Scienze Ambientali, Università della Tuscia, Viterbo, I-01100, Italy.
Baldassini
M R
MR
Mastrolia
L
L
Scapigliati
G
G
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001492:Bass / Q000276:immunology*; D015415:Biomarkers; D002241:Carbohydrates / Q000276:immunology; D000939:Epitopes; D006084:Graft Rejection / Q000276:immunology*; D007150:Immunohistochemistry; D008214:Lymphocytes / Q000276:immunology*; D018613:Microscopy, Confocal; D009132:Muscles / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous / Q000276:immunology / Q000473:pathology