S-(1,2-Dicarboxyethyl)glutathione in yeast: partial purification of its synthesizing enzyme.
S-(1,2-Dicarboxyethyl)glutathione (DCE-GS) was found in Saccharomyces cerevisiae, but not in bacterial species nor in a unicellular alga (Acetabularia acetabulum). The enzyme that catalyzes condensation of L-malate and glutathione (GSH) to form DCE-GS was partially purified from baker's yeast. It had a molecular mass of 49 kDa and was monomeric and the Km values were 2.2 and 1.4 mM for L-malate and GSH, respectively. The enzyme had a pH optimum of 7.5. DCE-GS levels in yeast cells were significantly higher in aerobic cultures than in anaerobic ones. DCE-GS was synthesized in cells cultured between 20 and 35 degrees C.
Tsuboi
S
S
Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Kiyono
K
K
Ono
B
B
Sakaue
T
T
Ogata
K
K
D000076:Acetabularia / Q000378:metabolism; D019883:Alkyl and Aryl Transferases / Q000302:isolation & purification* / Q000378:metabolism; D000595:Amino Acid Sequence; D001419:Bacteria / Q000378:metabolism; D004795:Enzyme Stability; D005978:Glutathione / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism; D005981:Glutathione Synthase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008293:Malates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature