Neuronal differentiation and patterning in Xenopus: the role of cdk5 and a novel activator xp35.2.
Cdk5, a member of the cyclin-dependent kinase family, has been shown to play an important role in development of the central nervous system in mammals when partnered by its activator p35. Here we describe the cloning and characterization of a novel activator of cdk5 in Xenopus, Xp35.2. Xp35.2 is expressed during development initially in the earliest differentiating primary neurons in the neural plate and then later in differentiating neural tissue of the brain. This is in contrast to the previously described Xenopus cdk5 activator Xp35.1 which is expressed over the entire expanse of the neural plate in both proliferating and differentiating cells. Expression of both Xp35.1 and Xp35.2 and activation of cdk5 kinase occur when terminal neural differentiation is induced by neurogenin and neuro D overexpression but not when only early stages of neural differentiation are induced by noggin. Moreover, blocking cdk5 kinase activity specifically results in disruption and reduction of the embryonic eye where cdk5 and its Xp35 activators are expressed. Thus, cdk5/p35 complexes function in aspects of neural differentiation and patterning in the early embryo and particularly in formation of the eye.
Deparment of Cell Biology, Department of Pathology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts, 02115, USA. ap113@hermes.cam.ac.uk
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Philpott
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Tsai
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Kirschner
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D002490:Central Nervous System / Q000196:embryology* / Q000254:growth & development; D003001:Cloning, Molecular; D051360:Cyclin-Dependent Kinase 5; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D047108:Embryonic Development; D004789:Enzyme Activation / Q000235:genetics*; D005123:Eye / Q000196:embryology* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D006651:Histocytochemistry; D017403:In Situ Hybridization; D008845:Microinjections; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics*; D009865:Oocytes / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014981:Xenopus / Q000196:embryology*; D029867:Xenopus Proteins
C106651:CDK5R1 protein, Xenopus; D009419:Nerve Tissue Proteins; D009479:Neuropeptides; D012333:RNA, Messenger; D029867:Xenopus Proteins; D051360:Cyclin-Dependent Kinase 5; C495908:Cdk5 protein, Xenopus; D018844:Cyclin-Dependent Kinases