Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis.
Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co-dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.
Flavell
A J
AJ
Department of Biochemistry, University of Dundee, UK. a.j.flavell@dundee.ac.uk
Knox
M R
MR
Pearce
S R
SR
Ellis
T H
TH
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018626:Retroelements / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers; D018626:Retroelements