Characterization and plasmid profile of an inhibitory strain of Erwinia herbicola isolated from Phaseolous vulgaris in Egypt.
Erwinia herbicola strain 48 was isolated from diseased phaseolous seedlings and characterized by biochemical properties, cellular fatty acid analysis and SDS-PAGE of the soluble cell protein. Although cellular fatty acid profile and the soluble cellular protein pattern showed high degree of similarity in comparison to those from E. herbicola strain 347417, obtained from the International Mycological Institute U.K., plasmid profiles were different. Both strains harbor a 23.1 kb plasmid, in addition, E. herbicola 48 contains 2 more plasmids (26.8 and 32.5 kb). The antagonism of E. herbicola 48 against a number of Gram-negative and Gram-positive bacteria was tested in vitro. Only Gram-negative bacteria were inhibited, suggesting that the inhibitory factor is likely to be bacteriocin.
Botany Department, Faculty of Science, Menofeya University, Egypt.
H H
HH
el-Hendawy
E A
EA
Azab
D000898:Antibiosis; D015373:Bacterial Typing Techniques; D004534:Egypt; D004885:Erwinia / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000254:growth & development; D010935:Plant Diseases / Q000382:microbiology; D010946:Plants, Medicinal; D010957:Plasmids / Q000235:genetics
D005227:Fatty Acids