Impaired neutrophil exocytosis in patients with severe pneumonia.
OBJECTIVE
Polymorphonuclear neutrophils (PMN) are one of the major effector cells of pulmonary defence against bacterial infection. To determine whether neutrophil function is impaired in patients with severe pneumonia, we assessed the two main partial functions exocytosis and oxidative response (ROS production) in isolated neutrophils from the peripheral venous blood of pneumonia patients and healthy volunteers. In addition, pulmonary neutrophils and peripheral neutrophils were compared in pneumonia patients.


METHODS
Twenty-one patients with severe pneumonia were enrolled in the study. Eleven patients were mechanically ventilated, ten patients breathed spontaneously. For comparison, ten healthy adults were studied. The release of two markers of neutrophil exocytosis, lactoferrin and myeloperoxidase (MPO), with and without stimulation by phorbol-myristate-acetate (PMA), was determined using immunoluminometric assays. ROS production was quantified using luminol-enhanced chemiluminescence. In addition, the clinical severity of pneumonia was correlated to neutrophil exocytosis.


RESULTS
With regard to blood neutrophils, both basal and PMA-stimulated exocytosis were significantly impaired in pneumonia patients compared to healthy volunteers (basal lactoferrin secretion in pneumonia patients: 0.25+/-0.36 pg/PMN versus controls: 1.17+/-0.78 pg/PMN, p<0.01). In contrast, both basal and PMA-stimulated ROS production were increased in patients compared to controls (spontaneous chemiluminescence in pneumonia patients: 13.6x10(5) cpm versus controls: 5.5x10(5) cpm). In pneumonia patients, the pulmonary neutrophils released significantly more lactoferrin, MPO and ROS compared to blood neutrophils (basal lactoferrin secretion of pulmonary neutrophils: 1.19+/-1.55 pg/PMN; p<0,01). However, after stimulation with PMA the exocytosis of pulmonary and blood neutrophils was similar. The severity of pneumonia and prognostic indices like albumin were inversely correlated to the release of lactoferrin in blood neutrophils (p<0,05).


CONCLUSIONS
In patients with severe pneumonia, the exocytosis of blood neutrophils was significantly impaired. In contrast to this, the oxidative response was increased. Impaired bone marrow maturation of neutrophils during severe infection, perhaps due to shortened maturation time, could explain these findings.
Zimmermann
B
B
Department of Medicine II, Medical University of Lübeck, Germany.
Dalhoff
K
K
Braun
J
J
D000328:Adult; D018893:Bronchoalveolar Lavage; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D005089:Exocytosis; D005260:Female; D006801:Humans; D007781:Lactoferrin / Q000097:blood; D008163:Luminescent Measurements; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism*; D009195:Peroxidase / Q000097:blood; D018410:Pneumonia, Bacterial / Q000097:blood* / Q000503:physiopathology* / Q000628:therapy; D012121:Respiration, Artificial; D012709:Serum Albumin / Q000378:metabolism; D012720:Severity of Illness Index
D012709:Serum Albumin; D002097:C-Reactive Protein; D009195:Peroxidase; D007781:Lactoferrin