Selective inhibition of the bacterial peptidoglycan biosynthesis by the new types of liposidomycins.
We examined the inhibitory activity against bacterial peptidoglycan biosynthesis, mammalian glycoprotein biosynthesis and growth of BALB/3T3 cells of four different types of liposidomycins which have the structure with or without sulfate and/or 3-methylglutaric acid moieties. Liposidomycins inhibited peptidoglycan biosynthesis about 30 to 500 times more effectively than tunicamycin, whereas liposidomycins inhibited mammalian glycoprotein biosynthesis about 30 to 300 times less effectively than tunicamycin. When the cytotoxic effect of liposidomycins and tunicamycin on the growth of mammalian cells were compared, liposidomycins did not show toxicity against BALB/3T3 cell at 25 microg/ml, though tunicamycin inhibited cell growth by 50% at 0.05 microg/ml. On the basis of these results, it is concluded that liposidomycins are selective antibiotics showing highly specific inhibition toward bacterial peptidoglycan biosynthesis.
Research Institute of Life Science, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
K
K
Kimura
Y
Y
Ikeda
S
S
Kagami
M
M
Yoshihama
K
K
Suzuki
H
H
Osada
K
K
Isono
D016475:3T3 Cells; D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D006023:Glycoproteins / Q000096:biosynthesis; D066298:In Vitro Techniques; D051379:Mice; D008826:Microbial Sensitivity Tests; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D015394:Molecular Structure; D010457:Peptidoglycan / Q000096:biosynthesis*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000378:metabolism; D051381:Rats; D014415:Tunicamycin / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D006023:Glycoproteins; D010457:Peptidoglycan; D011103:Polyisoprenyl Phosphate Monosaccharides; C117818:liposidomycin A-(I); C117819:liposidomycin A-(II); C117820:liposidomycin A-(III); C117821:liposidomycin A-(IV); C047544:liposidomycins; D014415:Tunicamycin; C018439:N-acetylglucosaminylpyrophosphoryldolichol