Identification and characterization of ligands for L-selectin in the kidney. II. Expression of chondroitin sulfate and heparan sulfate proteoglycans reactive with L-selectin.
Ligands for the leukocyte adhesion molecule L-selectin are expressed not only in lymph node high endothelial venules (HEV) but also in the renal distal tubuli. Here we report that L-selectin-reactive molecules in the kidney are chondroitin sulfate and heparan sulfate proteoglycans of 500-1000 kDa, unlike those in HEV bearing sialyl Lewis X-like carbohydrates. Binding of L-selectin to these molecules was mediated by the lectin domain of L-selectin and required divalent cations. Binding was inhibited by chondroitinase and/or heparitinase but not sialidase. Thus, L-selectin can recognize chondroitin sulfate and heparan sulfate glycosaminoglycans structurally distinct from sialyl Lewis X-like carbohydrates.
Li
Y F
YF
Department of Bioregulation, Biomedical Research Center, Osaka University Medical School, Suita, Japan.
Kawashima
H
H
Watanabe
N
N
Miyasaka
M
M
D000818:Animals; D002413:Cations, Divalent / Q000378:metabolism; D002809:Chondroitin Sulfates / Q000378:metabolism*; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism; D002850:Chromatography, Gel; D006497:Heparan Sulfate / Q000378:metabolism*; D007150:Immunohistochemistry; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D019041:L-Selectin / Q000378:metabolism*; D008024:Ligands; D008297:Male; D009844:Oligosaccharides / Q000276:immunology / Q000378:metabolism; D011133:Polysaccharide-Lyases / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
D002413:Cations, Divalent; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases; D006497:Heparan Sulfate; D019041:L-Selectin; D008024:Ligands; D009844:Oligosaccharides; D011133:Polysaccharide-Lyases; D011509:Proteoglycans; D011994:Recombinant Proteins; D000080506:Sialyl Lewis X Antigen; C021349:heparitinsulfate lyase