Functional importance and local environments of the cysteines in the tetracycline resistance protein encoded by plasmid pBR322.
The properties of the cysteines in the pBR322-encoded tetracycline resistance protein have been examined. Cysteines are important but not essential for tetracycline transport activity. None of the cysteines reacted with biotin maleimide, suggesting that they are shielded from the aqueous phase or reside in a negatively charged local environment.
Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071, USA.
J E
JE
Jewell
J
J
Orwick
J
J
Liu
K W
KW
Miller
D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry* / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics*; D002462:Cell Membrane / Q000737:chemistry / Q000648:ultrastructure; D017124:Conserved Sequence; D003545:Cysteine; D004251:DNA Transposable Elements; D004755:Enterobacteriaceae / Q000235:genetics*; D005838:Genotype; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010957:Plasmids; D017433:Protein Structure, Secondary; D011815:R Factors / Q000235:genetics*; D013752:Tetracycline / Q000493:pharmacokinetics; D013753:Tetracycline Resistance / Q000235:genetics*
D017920:Antiporters; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C079051:tetA protein, Bacteria; D013752:Tetracycline; D003545:Cysteine