Postdifferential display: parallel processing of candidates using small amounts of RNA.
The necessity of screening differentially expressed candidate genes has imposed a limit on the application of differential display to large-scale analysis of gene expression patterns. Screening candidates has indeed proven a burden because traditional screening methods require the purification of large amounts of RNA. In this article we describe an assay that allows the screening of 240 candidate genes with only 5 microg of total RNA. This assay consists of using cDNA probes synthesized from amplified RNA in differential screening and can be performed in a 96-well plate format.
Department of Anatomy, Medical School, Birmingham University, Edgbaston, United Kingdom. poirigmc@novell5.bham.ac.uk
G M
GM
Poirier
M G
MG
Erlander
D003001:Cloning, Molecular / Q000379:methods*; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010761:Phosphorus Radioisotopes; D012313:RNA / Q000235:genetics*; D014764:Viral Proteins
D017931:DNA Primers; D018076:DNA, Complementary; D010761:Phosphorus Radioisotopes; D014764:Viral Proteins; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases