[Characterization of the properties of differentially modified titanium surfaces for dental implantology. 2: In vitro biocompatibility studies].
The aim of the present study was to determine whether specific surface modifications are capable of improving the biocompatibility of a titanium surface, and whether there is a correlation between the physico-chemical properties of the implant material and its biocompatibility. To this end, the properties of titanium surfaces were modified using various methods or the latter were coated with various materials. Plasma treatments under different atmospheric conditions (N2-plasma, SO2-plasma, acetylene plasma) as well as plasma polymerization were used to affect the biological response. Characterization of the physico-chemical surface properties by means of X-ray photoelectron spectroscopy (XPS), contact angle measurements and the calculation of surface tensions or surface energy provided important information on the interactions at the interface between the implant material and the aqueous environment. The influence of the respective surface modification on cell proliferation, cell viability and the activity of mitochondrial dehydrogenases was evaluated in specific in vitro tests with human gingiva fibroblasts. It was show that different modifications of the titanium samples induce different biological responses of the gingiva fibroblasts. The results confirm the existence of correlations between thermodynamic surface properties and cellular reactions under in vitro conditions.
Klinik für Zahnärztliche Prothetik des Klinikums, RWTH Aachen.
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S
Säuberlich
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K
Liefeith
M
M
Frant
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D
Klee
E J
EJ
Richter
H
H
Höcker
H
H
Spiekermann
D002455:Cell Division; D002478:Cells, Cultured; D015921:Dental Implants; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology; D006801:Humans; D066298:In Vitro Techniques; D008422:Materials Testing; D013499:Surface Properties; D013500:Surface Tension; D014025:Titanium
D015921:Dental Implants; D014025:Titanium