Inhibition of galactose-induced cataractogenesis by troglitazone, a new antidiabetic drug with an antioxidant property, in rat lens culture.
Troglitazone is a new antidiabetic drug with a combined chemical structure of thiazolidinedione and alpha-tocopherol like structure (chroman ring). We evaluated the effect oftroglitazone on the morphological and biochemical changes in rat lenses cultured with galactose. Culturing in 30 mM galactose medium for 48 hrs resulted in vacuole formation in the cortex of lens equator (early phase of cataract). A significant amount of galactitol accumulation and lipid peroxide formation were also observed in lenses exposed to galactose. These morphological and biochemical changes associated with galactose were inhibited by 2 or 20 microM troglitazone present in the galactose medium. These results confirm the previous finding that troglitazone delayed the formation of cataract in rats fed a galactose diet. The anticataract effect of troglitazone was discussed in terms of antioxidant property of the drug.
Yokoyama
T
T
Pharmacology and Molecular Biology Research Laboratories, Sankyo Company Limited, Tokyo, Japan.
Yoshida
Y
Y
Inoue
T
T
Horikoshi
H
H
D000449:Aldehyde Reductase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002386:Cataract / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D002839:Chromans / Q000494:pharmacology*; D004376:Galactitol / Q000378:metabolism; D005690:Galactose; D005979:Glutathione Peroxidase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007908:Lens, Crystalline / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008054:Lipid Peroxides / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009924:Organ Culture Techniques; D009929:Organ Size; D051381:Rats; D017207:Rats, Sprague-Dawley; D013844:Thiazoles / Q000494:pharmacology*; D045162:Thiazolidinediones; D000077288:Troglitazone; D014617:Vacuoles / Q000187:drug effects
D000975:Antioxidants; D002839:Chromans; D007004:Hypoglycemic Agents; D008054:Lipid Peroxides; D013844:Thiazoles; D045162:Thiazolidinediones; D004376:Galactitol; D009249:NADP; D000449:Aldehyde Reductase; D005979:Glutathione Peroxidase; D005980:Glutathione Reductase; D000077288:Troglitazone; D005690:Galactose