Effect of meat (beef, chicken, and bacon) on rat colon carcinogenesis.
High intake of red meat or processed meat is associated with increased risk of colon cancer. In contrast, consumption of white meat (chicken) is not associated with risk and might even reduce the occurrence of colorectal cancer. We speculated that a diet containing beef or bacon would increase and a diet containing chicken would decrease colon carcinogenesis in rats. One hundred female Fischer 344 rats were given a single injection of azoxymethane (20 mg/kg i.p.), then randomized to 10 different AIN-76-based diets. Five diets were adjusted to 14% fat and 23% protein and five other diets to 28% fat and 40% protein. Fat and protein were supplied by 1) lard and casein, 2) olive oil and casein, 3) beef, 4) chicken with skin, and 5) bacon. Meat diets contained 30% or 60% freeze-dried fried meat. The diets were given ad libitum for 100 days, then colon tumor promotion was assessed by the multiplicity of aberrant crypt foci [number of crypts per aberrant crypt focus (ACF)]. The ACF multiplicity was nearly the same in all groups, except bacon-fed rats, with no effect of fat and protein level or source (p = 0.7 between 8 groups by analysis of variance). In contrast, compared with lard- and casein-fed controls, the ACF multiplicity was reduced by 12% in rats fed a diet with 30% bacon and by 20% in rats fed a diet with 60% bacon (p < 0.001). The water intake was higher in bacon-fed rats than in controls (p < 0.0001). The concentrations of iron and bile acids in fecal water and total fatty acids in feces changed with diet, but there was no correlation between these concentrations and the ACF multiplicity. Thus the hypothesis that colonic iron, bile acids, or total fatty acids can promote colon tumors is not supported by this study. The results suggest that, in rats, beef does not promote the growth of ACF and chicken does not protect against colon carcinogenesis. A bacon-based diet appears to protect against carcinogenesis, perhaps because bacon contains 5% NaCl and increased the rats' water intake.
Laboratoire Sécurité des Aliments, Institut National de la Recherche Agronomique, Ecole Nationale Vétérinaire, Toulouse, France.
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D000273:Adipose Tissue / Q000033:anatomy & histology; D000818:Animals; D001397:Azoxymethane; D001647:Bile Acids and Salts / Q000032:analysis; D002273:Carcinogens; D002417:Cattle; D002645:Chickens; D003106:Colon / Q000033:anatomy & histology; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000209:etiology* / Q000517:prevention & control; D004032:Diet; D004326:Drinking; D004435:Eating; D005227:Fatty Acids / Q000032:analysis; D005243:Feces / Q000737:chemistry; D005260:Female; D007501:Iron / Q000032:analysis; D008460:Meat / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D012307:Risk Factors; D013552:Swine; D014781:Viscera; D015430:Weight Gain
D001647:Bile Acids and Salts; D002273:Carcinogens; D005227:Fatty Acids; D007501:Iron; D001397:Azoxymethane