A model for parallel triple helix formation by RecA: single-single association with a homologous duplex via the minor groove.
The nucleoproteic filaments of RecA polymerized on single stranded DNA are able to integrate double stranded DNA in a coaxial arrangement (with DNA stretched by a factor 1.5), to recognize homologous sequences in the duplex and to perform strand exchange between the single stranded and double stranded molecules. While experimental results favor the hypothesis of an invasion of the minor groove of the duplex by the single strand, parallel minor groove triple helices have never been isolated or even modeled, the minor groove offering little space for a third strand to interact. Based on an internal coordinate modeling study, we show here that such a structure is perfectly conceivable when the two interacting oligomers are stretched by a factor 1.5, in order to open the minor groove of the duplex. The model helix presents characteristics that coincide with known experimental data on unwinding, base pair inclination and inter-proton distances. Moreover, we show that extension and unwinding stabilize the triple helix. New patterns of triplet interaction via the minor groove are presented.
Laboratoire de Biochimie Théorique, UPR 9080, Institut de Biologie Physico-Chimique, Paris, France.
G
G
Bertucat
R
R
Lavery
C
C
Prévost
D003198:Computer Simulation; D007700:Kinetics; D008958:Models, Molecular; D015233:Models, Statistical; D008968:Molecular Conformation; D011938:Rec A Recombinases / Q000737:chemistry* / Q000378:metabolism*
D011938:Rec A Recombinases