Bi-PASA genotyping of a new polymorphism in the APOB gene shows no evidence for an association with fatness in pigs.
Sequence analysis of PCR products of a 343-bp fragment from exon 29 of the porcine APOB gene of four Erhualian and four Landrace pigs revealed a missense G/C substitution at position of 6117 in this gene. Two allele-specific primers were designed to genotype this polymorphism using the Bi-PASA technique. Genotyping of 146 animals from Erhualian, Hampshire, Large White, Landrace and Duroc breeds revealed large breed differences in allele frequency. No association with fatness was observed within each of the four European breeds, where animals had been selected to be those with the highest and those with the lowest backfat depth at 100 kg liveweight from a large carcass dissection project.
Department of Animal and Poultry Science, University of Guelph, Ontario, Canada.
Z H
ZH
Jiang
J P
JP
Gibson
D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics*; D005787:Gene Frequency; D005838:Genotype; D020125:Mutation, Missense; D009765:Obesity / Q000235:genetics / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D011110:Polymorphism, Genetic; D013552:Swine / Q000235:genetics*
D001055:Apolipoproteins B