SRPK1 and LBR protein kinases show identical substrate specificities.
Arginine/serine protein kinases constitute a novel class of enzymes that can modify arginine/serine (RS) dipeptide motifs. SR splicing factors that are essential for pre-mRNA splicing and the lamin B receptor (LBR), an integral protein of the inner nuclear membrane, are among the best characterized proteins that contain RS domains. Two SR Protein-specific Kinases, SRPK1 and SRPK2, have been shown to phosphorylate specifically the RS motifs of the SR family of splicing factors and play an important role in regulating both the spliceosome assembly and their intranuclear distribution, whereas an LBR-associated kinase, that specifically phosphorylates a stretch of RS repeats located at the NH2-terminal region of LBR, has been recently purified and characterized from turkey erythrocyte nuclear envelopes. Using synthetic peptides representing different regions of LBR and recombinant proteins produced in bacteria we now demonstrate that SRPK1 modifies LBR with similar kinetics and on the same sites as the LBR kinase, that are also phosphorylated in vivo. These data provide significant evidence for a new role of SRPK1 in addition to that of pre-mRNA splicing.
Laboratory of Biochemistry, School of Chemistry, Aristotelian University of Thessaloniki, Thessaloniki, 54 006, Greece.
S
S
Papoutsopoulou
E
E
Nikolakaki
T
T
Giannakouros
D000595:Amino Acid Sequence; D006801:Humans; D007700:Kinetics; D008969:Molecular Sequence Data; D009685:Nuclear Envelope / Q000201:enzymology; D009687:Nuclear Proteins / Q000378:metabolism; D010748:Phosphopeptides / Q000032:analysis; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing / Q000235:genetics; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism; D000097682:Lamin B Receptor
D009687:Nuclear Proteins; D010748:Phosphopeptides; D017346:Protein Serine-Threonine Kinases; D012322:RNA Precursors; D018160:Receptors, Cytoplasmic and Nuclear; D011994:Recombinant Proteins; D014357:Trypsin; D000097682:Lamin B Receptor; C087728:SRPK1 protein, human