Enhancement of phagocytic and chemokinetic activities of rainbow trout head kidney cells by C-reactive protein.
OBJECTIVE
To investigate immunostimulating activity of purified rainbow trout (Oncorhynchus mykiss) C-reactive protein (CRP) on trout phagocytic cells.


METHODS
20 rainbow trout and 2 rabbits.


METHODS
The effect of CRP on phagocytic activity of head kidney (HK) cells was examined by use of a phagocytosis assay with plastic particles. The enhancing effect of CRP on migration activity of HK cells was examined by use of the blind well assay.


RESULTS
Glass-adherent cells from clinically normal trout had increased dose-dependent phagocytic activity against plastic particles when cells were incubated in the presence of CRP. Pretreatment of particles with CRP also enhanced phagocytic activity of the cells, indicating an opsonic effect of CRP. Rabbit anti-trout CRP serum suppressed the enhancing activity of CRP. The HK cells had significant dose-dependent chemokinetic activity against CRP that was not inhibited by anti-CRP serum, indicating that a CRP-antibody complex also could be chemokinetic.


CONCLUSIONS
Rainbow trout CRP has immunostimulating activity for HK cells, resulting in enhanced phagocytic and chemokinetic activities.
Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Sakai, Japan.
H
H
Kodama
H
H
Arimitsu
M
M
Mukamoto
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C
Sugimoto
D000818:Animals; D002097:C-Reactive Protein / Q000276:immunology* / Q000302:isolation & purification; D002465:Cell Movement / Q000276:immunology / Q000502:physiology; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange / Q000662:veterinary; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D007106:Immune Sera / Q000096:biosynthesis; D005779:Immunodiffusion / Q000662:veterinary; D007668:Kidney / Q000166:cytology; D008863:Microspheres; D017686:Oncorhynchus mykiss / Q000276:immunology*; D009895:Opsonin Proteins / Q000737:chemistry / Q000276:immunology; D010586:Phagocytes / Q000502:physiology*; D010587:Phagocytosis / Q000502:physiology*; D011817:Rabbits; D017421:Sequence Analysis
D007106:Immune Sera; D009895:Opsonin Proteins; D002097:C-Reactive Protein