Induction of Sarcophaga central nervous system remodeling by 20-hydroxyecdysone in vitro.
Proliferation and apoptosis of neural cells were found to be induced simultaneously when larval brains of Sarcophaga peregrina were cultured in the presence of 20-hydroxyecdysone (20-HE) for 24 h. The locations of proliferating cells and apoptotic cells in the brain hemispheres were different. The morphology of brains exposed to 20-HE for a short period proceeded to change sequentially when culture was continued for 2 days even in the absence of 20-HE. These changes mainly consisted of enlargement of the brain hemispheres and extension of the interval between two hemispheres, which closely paralleled the morphological changes of brains that occur in the early pupal stage, suggesting that ecdysteroid alone is sufficient to induce the remodeling of the central nervous system of holometabolous insects. Synthesis of a protein with a molecular mass of 66 kDa was shown to be selectively repressed when brains were cultured in the presence of 20-HE.
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-0033, Japan.
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I
Fujii
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Y
Tanaka
K i
Ki
Homma
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Natori
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002490:Central Nervous System / Q000187:drug effects* / Q000473:pathology*; D004441:Ecdysterone / Q000494:pharmacology*; D007313:Insecta; D009474:Neurons / Q000187:drug effects* / Q000473:pathology*; D009924:Organ Culture Techniques
D004441:Ecdysterone