A lipid modified ubiquitin is packaged into particles of several enveloped viruses.
An anti-ubiquitin cross-reactive protein which migrates more slowly (6.5 kDa) by SDS-PAGE than ubiquitin was identified in African swine fever virus particles. This protein was extracted into the detergent phase in Triton X-114 phase separations, showing that it is hydrophobic, and was radiolabelled with both [3H]palmitic acid and [32P]orthophosphate. This indicates that the protein has a similar structure to the membrane associated phosphatidyl ubiquitin described in baculovirus particles. A similar molecule was found in vaccinia virus and herpes simplex virus particles, suggesting that it may be a component of uninfected cell membranes, which is incorporated into membrane layers in virions during morphogenesis.
Institute for Animal Health, Pirbright Laboratory, Woking, UK.
J H
JH
Webb
R J
RJ
Mayer
L K
LK
Dixon
D000358:African Swine Fever Virus / Q000737:chemistry; D000818:Animals; D016367:Baculoviridae / Q000737:chemistry; D015153:Blotting, Western; D002460:Cell Line; D002522:Chlorocebus aethiops; D019281:Dimerization; D004591:Electrophoresis, Polyacrylamide Gel; D008055:Lipids / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D008970:Molecular Weight; D017830:Octoxynol; D019308:Palmitic Acid / Q000032:analysis; D010710:Phosphates / Q000032:analysis; D011092:Polyethylene Glycols; D011233:Precipitin Tests; D018139:Simplexvirus / Q000737:chemistry; D014452:Ubiquitins / Q000031:analogs & derivatives / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D014616:Vaccinia virus / Q000737:chemistry; D014764:Viral Proteins / Q000032:analysis* / Q000737:chemistry; D014771:Virion / Q000737:chemistry*
D008055:Lipids; D008565:Membrane Proteins; D010710:Phosphates; D014452:Ubiquitins; D014764:Viral Proteins; D019308:Palmitic Acid; D011092:Polyethylene Glycols; D017830:Octoxynol; C010615:Nonidet P-40