Activation of integrin-beta3-associated syk in platelets.
Published data suggest that the tyrosine kinase syk participates in platelet signalling through the integrin alphaIIbbeta3. Our data show an association of syk and integrin beta3 in immunoprecipitates from unstimulated and stimulated platelets. We detected syk in anti-beta3 precipitates and, conversely, beta3 in anti-syk precipitates. In vitro kinase assays with anti-beta3 precipitates demonstrated that syk activity was enhanced in ADP-stimulated platelets.
Department of Pathology and Laboratory Medicine, University of North Carolina, Chapel Hill, NC 27599, USA.
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Sarkar
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Rooney
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Lord
D015703:Antigens, CD / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism*; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D039661:Integrin beta3; D047908:Intracellular Signaling Peptides and Proteins; D010766:Phosphorylation; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism*; D011233:Precipitin Tests; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D000072377:Syk Kinase
D015703:Antigens, CD; D004792:Enzyme Precursors; D039661:Integrin beta3; D047908:Intracellular Signaling Peptides and Proteins; D010980:Platelet Membrane Glycoproteins; D011505:Protein-Tyrosine Kinases; C000606896:SYK protein, human; D000072377:Syk Kinase