High-performance liquid chromatographic assay with a simple extraction procedure for sensitive quantification of mycophenolic acid in rat and human plasma.
We describe a novel sensitive and simplified gradient HPLC assay for quantification of the immunosuppressant mycophenolic acid (MPA) in rat and human plasma. In contrast to previously reported MPA assays, our method used a single step extraction comprising addition of acetonitrile, which contained phenolphthalein glucoronic acid as internal standard, for protein precipitation. Linearity: 0.1-100 microg/ml (r2>0.999), mean recoveries: MPA 98.0%, internal standard 105.2%, mean intra-day precision: 4.3%, mean day-to-day precision: 4.3%, mean day-to-day accuracy: -1.5%. Sensitivity was sufficient to allow for quantification of mycophenolic acid in as little as 50 microl plasma.
Transplantation Immunology, Department of Cardiothoracic Surgery, Stanford University Medical School, CA 94305-5247, USA.
J F
JF
Gummert
U
U
Christians
M
M
Barten
H
H
Silva
R E
RE
Morris
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D008297:Male; D051379:Mice; D009173:Mycophenolic Acid / Q000097:blood*; D051381:Rats; D011917:Rats, Inbred Lew; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D007166:Immunosuppressive Agents; D009173:Mycophenolic Acid