All 16 centromere DNAs from Saccharomyces cerevisiae show DNA curvature.
All 16 centromere DNA regions of Saccharomyces cerevisiae including 90 bp framing sequences on either side were cloned. These 300 bp long centromere regions were analysed by native polyacrylamide gel electrophoresis and found to display a reduced mobility indicative of DNA curvature. The degree of curvature is centromere dependent. The experimental data were confirmed by computer analysis of the 3-dimensional structure of the CEN DNAs. Altogether these data provide further evidence for a model for budding yeast centromeres in which CEN DNA structure could be important for the assembly, activity and/or regulation of the centromere protein-DNA complex.
Bechert
T
T
Abteilung Molekularbiologie, Institut für Molekulare Biotechnologie, Postfach 100813, 07708 Jena, Germany and Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen, Frankfurter Strasse 107,35392 Giessen.
Heck
S
S
Fleig
U
U
Diekmann
S
S
Hegemann
J H
JH
D001483:Base Sequence; D002503:Centromere / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D003198:Computer Simulation; D004271:DNA, Fungal / Q000737:chemistry* / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004996:Ethidium / Q000494:pharmacology; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013696:Temperature
D004271:DNA, Fungal; D004996:Ethidium