Three kinds of guanylate cyclase expressed in medaka photoreceptor cells in both retina and pineal organ.
Three kinds of cDNAs encoding the putative photoreceptor-specific guanylate cyclases (GCs), OlGC-R1, OlGC-R2, and OlGC-C, were isolated from a retinal cDNA library of the medaka, Oryzias latipes. The deduced amino acid sequences of OlGC-R1 and -C are closely related but slightly different from those of OlGC4 and OlGC5, respectively. In situ hybridization locates the mRNA of both OlGC-R1 and -R2 in rods, and OlGC-C mRNA is found in all four types of cone cells. It is likely that medaka rods and cones produce distinct GC subtypes and that two kinds of photoreceptor-specific GCs are coexpressed in rods. Also, hybridization signals are detected in the pineal organ, suggesting that OlGC-R1 and -C contribute also to phototransduction in pinealocytes.
Hisatomi
O
O
Graduate School of Science, Osaka University, Japan.
Honkawa
H
H
Imanishi
Y
Y
Satoh
T
T
Tokunaga
F
F
D000595:Amino Acid Sequence; D000818:Animals; D006162:Guanylate Cyclase / Q000096:biosynthesis* / Q000235:genetics; D017403:In Situ Hybridization; D007527:Isoenzymes / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D009990:Oryzias; D020419:Photoreceptor Cells, Vertebrate / Q000201:enzymology*; D010870:Pineal Gland / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000737:chemistry / Q000166:cytology / Q000201:enzymology*
D007527:Isoenzymes; D012333:RNA, Messenger; D006162:Guanylate Cyclase