Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin.
Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.
Shibahara
K
K
Cold Spring Harbor Laboratory, New York 11724, USA.
Stillman
B
B
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001323:Autoantibodies / Q000494:pharmacology; D002455:Cell Division / Q000235:genetics; D002843:Chromatin / Q000235:genetics* / Q000378:metabolism; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004261:DNA Replication / Q000187:drug effects / Q000235:genetics*; D004278:DNA, Superhelical / Q000187:drug effects; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D006367:HeLa Cells; D018398:Homeodomain Proteins; D006801:Humans; D015778:Minor Histocompatibility Antigens; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D019253:Proto-Oncogene Proteins c-bcl-2; D051818:Replication Protein C; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D013539:Simian virus 40 / Q000235:genetics; D013696:Temperature; D014157:Transcription Factors
D001323:Autoantibodies; C096816:BCL2-related protein A1; C533593:CHAF1B protein, human; C489780:CNOT8 protein, human; D002843:Chromatin; D056504:Chromatin Assembly Factor-1; D002868:Chromosomal Proteins, Non-Histone; D004278:DNA, Superhelical; D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C096063:MATA1 protein, S cerevisiae; D015778:Minor Histocompatibility Antigens; D018809:Proliferating Cell Nuclear Antigen; D019253:Proto-Oncogene Proteins c-bcl-2; D012097:Repressor Proteins; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D000255:Adenosine Triphosphate; D051818:Replication Protein C