Diatom motility and low frequency electromagnetic fields--a new technique in the search for independent replication of results.
The hypothesis that exposure to a certain combination of static and alternating electromagnetic fields (EMFs) results in an increase in motility of the marine diatom Amphora coffeaeformis was tested. Diatom motility in three strains of A. coffeaeformis was positively correlated with extracellular calcium ion (Ca2+) concentration. The test apparatus consisted of two pairs of Helmholtz coils supported around the stage of a microscope linked to a video recorder and monitor. This system allowed real-time in vivo recordings of diatom speed under EMF and control exposures. The EMFs were calculated at calcium resonance values, previously found to cause enhanced motility. Computerised image analysis was used to calculate the distance moved by individual diatoms in 2-min periods before, during and after EMF or sham-EMF (control) exposure. The addition of EMF caused no significant increase in diatom motility. The results are discussed in relation to the use of diatom motility to measure EMF exposure effects.
Ecology Centre, University of Sunderland, UK. n.clarkson@livjm.ac.uk
N
N
Clarkson
M S
MS
Davies
R
R
Dixey
D002118:Calcium / Q000378:metabolism; D002452:Cell Count; D002465:Cell Movement / Q000528:radiation effects; D017377:Diatoms / Q000166:cytology / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D004574:Electromagnetic Fields; D004578:Electron Spin Resonance Spectroscopy; D007091:Image Processing, Computer-Assisted; D018715:Microscopy, Video; D011829:Radiation Dosage; D014743:Videotape Recording
D002118:Calcium