hMSH5: a human MutS homologue that forms a novel heterodimer with hMSH4 and is expressed during spermatogenesis.
MutS homologues have been identified in nearly all organisms examined to date. They play essential roles in maintaining mitotic genetic fidelity and meiotic segregation fidelity. MutS homologues appear to function as a molecular switch that signals genomic manipulation events. Here we describe the identification of the human homologue of the Saccharomyces cerevisiae MSH5, which is known to participate in meiotic segregation fidelity and crossing-over. The human MSH5 (hMSH5) was localized to chromosome 6p22-21 and appears to play a role in meiosis because expression is induced during spermatogenesis between the late primary spermatocytes and the elongated spermatid phase. hMSH5 interacts specifically with hMSH4, confirming the generality of functional heterodimeric interactions in the eukaryotic MutS homologue, which also includes hMSH2-hMSH3 and hMSH2-hMSH6.
Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University and Medical College, Philadelphia, Pennsylvania 19107, USA.
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Bocker
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Barusevicius
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Snowden
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Rasio
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Guerrette
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Robbins
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Schmidt
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Burczak
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Croce
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Copeland
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Kovatich
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Fishel
D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008297:Male; D008540:Meiosis; D008969:Molecular Sequence Data; D029701:Saccharomyces cerevisiae Proteins; D013091:Spermatogenesis
D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C091158:MSH4 protein, S cerevisiae; C094801:MSH5 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins