Transfer of a protein binding epitope to a minimal designed peptide.
Results from protein mutagenesis and x-ray crystallographic studies of the multidomain protein Vascular Cell Adhesion Molecule (VCAM) were used to design cyclic octapeptides that retain the critical structural and binding elements of the epitope of VCAM in the interaction with the integrin alpha 4 beta 1 (VLA-4). Changes in the activities of peptide analogues correlated with the relative activities of protein mutants of VCAM, and predicted the properties of two new mutants that bound alpha 4 beta 1 with improved affinity vs wild type protein. The nmr structures of two peptides revealed a high degree of similarity to the structure of the VCAM binding epitope. These results demonstrate that a compact binding epitope identified via protein structure-function studies may be transferred to a synthetically accessible small peptide with the key structure-activity relationships intact.
Quan
C
C
Department of Bioorganic Chemistry, Genentech, Inc., South San Francisco, CA 94080, USA.
Skelton
N J
NJ
Clark
K
K
Jackson
D Y
DY
Renz
M E
ME
Chiu
H H
HH
Keating
S M
SM
Beresini
M H
MH
Fong
S
S
Artis
D R
DR
D001665:Binding Sites; D001667:Binding, Competitive; D000939:Epitopes / Q000737:chemistry*; D039041:Integrin alpha4beta1; D016023:Integrins / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008727:Methotrexate / Q000737:chemistry; D008958:Models, Molecular; D010446:Peptide Fragments / Q000737:chemistry; D010456:Peptides, Cyclic / Q000737:chemistry*; D011485:Protein Binding; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D013329:Structure-Activity Relationship; D019010:Vascular Cell Adhesion Molecule-1 / Q000737:chemistry
D000939:Epitopes; D039041:Integrin alpha4beta1; D016023:Integrins; D010446:Peptide Fragments; D010456:Peptides, Cyclic; D016201:Receptors, Lymphocyte Homing; D019010:Vascular Cell Adhesion Molecule-1; D008727:Methotrexate