High-performance liquid chromatographic determination of paclitaxel in rat serum: application to a toxicokinetic study.
A simple and sensitive high-performance liquid chromatographic method is described for the determination of paclitaxel (Taxol) at 230 nm using a Nucleosil C18 (5 microm) column and a methanol-water (70:30, v/v) mobile phase following a single-step extraction from serum with dichloromethane. The assay was validated against the classical criteria and was applied to a toxicokinetic study in rats after one or five, one per week) intraperitoneal administrations of 16 mg/kg Taxol.
Laboratoire de Toxicologie, Equipe NPPUA, Faculté de Pharmacie, Clermont-Ferrand, France.
F
F
Coudoré
N
N
Authier
D
D
Guillaume
A
A
Béal
E
E
Duroux
J
J
Fialip
D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000097:blood* / Q000493:pharmacokinetics* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D007274:Injections, Intraperitoneal; D017239:Paclitaxel / Q000097:blood* / Q000493:pharmacokinetics* / Q000633:toxicity; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results
D000972:Antineoplastic Agents, Phytogenic; D017239:Paclitaxel