Simplified diagnosis of malaria infection: GFM/PCR/ELISA a simplified nucleic acid amplification technique by PCR/ELISA.
We report an adaptation of a technique for the blood sample collection (GFM) as well as for the extraction and amplification of Plasmodium DNA for the diagnosis of malaria infection by the PCR/ELISA. The method of blood sample collection requires less expertise and saves both time and money, thus reducing the cost by more than half. The material is also suitable for genetic analysis in either fresh or stored specimens prepared by this method.
Machado
R L
RL
Laboratório de Malária, Instituto Evandro Chagas, Belém, Pará, Brasil.
Garret
D O
DO
Adagu
I S
IS
Warhurst
D C
DC
Póvoa
M M
MM
D000818:Animals; D001800:Blood Specimen Collection / Q000191:economics / Q000379:methods*; D016054:DNA, Protozoan / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D008288:Malaria / Q000175:diagnosis*; D010961:Plasmodium / Q000235:genetics; D016133:Polymerase Chain Reaction
D016054:DNA, Protozoan