Effects of erythromycin on experimental extrinsic allergic alveolitis.
BACKGROUND
Recent clinical studies have demonstrated the efficacy of erythromycin for treating patients with chronic lower respiratory tract inflammation. Mechanisms related to the anti-inflammatory action are yet to be determined.


OBJECTIVE
The therapeutic efficacy of erythromycin in experimental extrinsic allergic alveolitis (EAA) was evaluated.


METHODS
A murine model of EAA was developed by intratracheal inoculations with particulate Trichosporon mucoides followed by erythromycin or josamycin treatment. Cell populations, specific antibodies, chemotactic activities, TNF-alpha, IL-1beta, MIP-2 and KC of bronchoalveolar lavage fluid (BALF); histopathology of the lung and footpad reaction; myeloperoxidase of the whole lung; and immunohistochemistry of intercellular adhesion molecule- (ICAM-1), at 6 and 96 h after the challenge, were examined.


RESULTS
There was a marked neutrophilic alveolitis and bronchiolitis at 6 h, and lymphocytic alveolitis and perivenule cuffing at 96 h after the challenge. Increase in total inflammatory cells and neutrophils in BALF at 6h was significantly suppressed by pretreatment with 5 mg/kg/day of erythromycin intraperitoneally for 5 days (P<0.01), with no apparent effect on specific antibodies, chemotactic activity or cytokines. Erythromycin also suppressed the Arthus-type reaction in the footpad (P<0.01). Histopathological studies revealed that erythromycin markedly decreased neutrophils in the lung and skin lesions and myeloperoxidase in the lung, simultaneously with inhibiting ICAM-1 expression. The therapy has no remarkable effects on lymphocytes or 96 h response. Josamycin had no effects on the model.


CONCLUSIONS
The therapeutic dosage of erythromycin significantly suppressed acute neutrophil influx into the lung, intradermal Arthus reaction and the expression of ICAM-1 in the lesions of experimental EAA. Erythromycin may be effective for treating subjects with acute EAA.
First Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
M
M
Miyajima
M
M
Suga
K
K
Nakagawa
K
K
Ito
M
M
Ando
D000542:Alveolitis, Extrinsic Allergic / Q000188:drug therapy* / Q000378:metabolism / Q000382:microbiology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001183:Arthus Reaction / Q000517:prevention & control; D001992:Bronchoalveolar Lavage Fluid; D019742:Chemokines, CC; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004917:Erythromycin / Q000627:therapeutic use*; D005260:Female; D007124:Immunoenzyme Techniques; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019402:Macrophage Inflammatory Proteins; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects; D009195:Peroxidase / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D014250:Trichosporon
D000900:Anti-Bacterial Agents; C095218:Ccl9 protein, mouse; D019742:Chemokines, CC; D016207:Cytokines; D019402:Macrophage Inflammatory Proteins; D018799:Intercellular Adhesion Molecule-1; D004917:Erythromycin; D009195:Peroxidase