Asymmetric distribution of phosphatidylethanolamine in C. albicans: possible mediation by CDR1, a multidrug transporter belonging to ATP binding cassette (ABC) superfamily.
By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans, is present in its external half. Evidence is presented to show that the availability of PtdEtn could be related to the expression of a multidrug transporter CDR1 of C. albicans, and the process is energy-dependent. A homozygous CDR1 disruptant strain of C. albicans shows almost 23% reduction in the external labelling of PtdEtn. This report shows that, similar to human MDRs, yeast multidrug transporter could also be involved in aminophospholipid translocation.
Dogra
S
S
Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Krishnamurthy
S
S
Gupta
V
V
Dixit
B L
BL
Gupta
C M
CM
Sanglard
D
D
Prasad
R
R
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D005450:Fluorescamine / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D010714:Phosphatidylethanolamines / Q000032:analysis* / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; C094693:CDR1 protein, Candida albicans; D005656:Fungal Proteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010714:Phosphatidylethanolamines; D005450:Fluorescamine; D000255:Adenosine Triphosphate; D014302:Trinitrobenzenesulfonic Acid