Expression of atrC - encoding a novel member of the ATP binding cassette transporter family in Aspergillus nidulans - is sensitive to cycloheximide.
A new member of the ABC superfamily of transmembrane proteins in Aspergillus nidulans has been cloned and characterized. The topology of conserved motifs subgroups AtrC in the P-glycoprotein cluster of ABC permeases, the members of this subfamily, are known to participate in multidrug resistance (MDR) in diverse organisms. Alignment results display significant amino acid similarity to AfuMDR1 and AflMDR1 from Aspergillus fumigatus and flavus, respectively. Northern analysis reveals that atrC mRNA levels are 10-fold increased in response to cycloheximide. Evidence for the existence of eight additional hitherto unpublished ABC transporter proteins in A. nidulans is provided.
Department of Microbiology, University of Innsbruck, Medical School, Fritz-Pregl-Str. 3, A-6020, Innsbruck, Austria. klaus.angermayr@uibk.ac.at
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K
Angermayr
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W
Parson
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G
Stöffler
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H
Haas
D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D000595:Amino Acid Sequence; D001233:Aspergillus nidulans / Q000187:drug effects / Q000235:genetics*; D001483:Base Sequence; D003513:Cycloheximide / Q000494:pharmacology*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D008969:Molecular Sequence Data; D016415:Sequence Alignment
D018528:ATP-Binding Cassette Transporters; C117988:AtrC protein, Aspergillus nidulans; D005656:Fungal Proteins; D003513:Cycloheximide