Neurotransmission in the medulla mediating insular cortical and lateral hypothalamic sympathetic responses.
Previous evidence has shown sympathetic nerve responses to insular cortical (IC) stimulation are mediated by synapses within the lateral hypothalamic area (LHA) and ventrolateral medulla (VLM). The present study was aimed at determining the neurotransmitter(s) and receptor(s) involved at the synapse in the VLM. Twenty male Wistar rats were instrumented for renal nerve, arterial pressure, and heart rate recording. The IC or the LHA was stimulated with a bipolar electrode (200-1000 microA; 2 ms; 0.8 Hz) to elicit sympathetic nerve responses. Antagonists were then pressure-injected into the VLM (300 nL). Bilateral and unilateral kynurenate (25 mM) resulted in 100% block of IC-and LHA-stimulated sympathetic nerve responses. Bilateral injection of the non-NMDA (N-methyl-D-aspartate) receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 200 microM) also resulted in up to 100% block of IC and LHA sympathetic responses. In addition, unilateral injections of CNQX were made in two animals, resulting in 100 and 83% block of LHA sympathetic responses. Bilateral injection of the NMDA receptor antagonist DL-2-amino-5-phosphonopentanoic acid (AP5; 200 microM) did not affect the response to IC or LHA stimulation. Kynurenate, CNQX, and AP5 all resulted in an elevation of baseline sympathetic nerve activity and a pressor response. Kynurenate resulted in a 263+/-79% increase in baseline activity, while CNQX and AP5 resulted in 83+/-19% and 91+/-21% increases. respectively. Bilateral injections of antagonists for GABA(A) (bicuculline; 0.1 microM), acetylcholine (atropine; 0.1 microM) and catecholaminergic alpha and beta receptors (phentolamine and propranolol: 0.1 microM) had no effect on LHA sympathetic responses. Thus, sympathetic responses originating in the IC and LHA are mediated by a non-NMDA receptors in the VLM, which are likely AMPA receptors.
Department of Anatomy, The University of Western Ontario, London, Canada.
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Butcher
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Cechetto
D000200:Action Potentials; D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002395:Catecholamines / Q000037:antagonists & inhibitors; D002540:Cerebral Cortex / Q000502:physiology*; D004558:Electric Stimulation; D005071:Evoked Potentials; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D006339:Heart Rate / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D007668:Kidney / Q000502:physiology; D008297:Male; D008526:Medulla Oblongata / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017981:Receptors, Neurotransmitter / Q000187:drug effects / Q000502:physiology*; D013238:Stereotaxic Techniques; D013564:Sympathetic Nervous System / Q000502:physiology*
D002395:Catecholamines; D018691:Excitatory Amino Acid Antagonists; D018377:Neurotransmitter Agents; D017981:Receptors, Neurotransmitter