Detection of PCR products in solution using surface plasmon resonance.
Polymerase chain reaction (PCR) products were detected using a flow injection-type sensor based on surface plasmon resonance. Asymmetric PCR was used to amplify the target DNA sequence, and two products with different length were produced. The novelty of our DNA detection system was that our target DNA was double stranded but the probe binding site, located in the 3'-terminus, was single stranded. This avoids the formation of intra- and intermolecular complexes. This novel design permitted us not only to detect PCR product but also to develop a rapid detection system for the detection of the verotoxin 2 gene of Escherichia coli O157:H7.
Research Center for Advanced Science and Technology, University of Tokyo, Japan.
E
E
Kai
S
S
Sawata
K
K
Ikebukuro
T
T
Iida
T
T
Honda
I
I
Karube
D001427:Bacterial Toxins / Q000032:analysis / Q000235:genetics; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004926:Escherichia coli / Q000235:genetics; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D022641:Shiga Toxin 2; D012996:Solutions; D020349:Surface Plasmon Resonance / Q000379:methods*; D013696:Temperature
D001427:Bacterial Toxins; D022641:Shiga Toxin 2; D012996:Solutions; D004247:DNA