Quantitative assessment of DNA condensation.
A fluorescent method is proposed for assessing DNA condensation in aqueous solutions with variety of condensing agents. The technique is based on the effect of concentration-dependent self-quenching of covalently bound fluorophores upon DNA collapse. The method allows a more precise determination of charge equivalency in titration experiments with various polycations. The technique's ability to determine the number of DNA molecules that are condensed together in close proximity is under further investigation.
Trubetskoy
V S
VS
Mirus Corporation, Madison, Wisconsin 53711, USA.
Slattum
P M
PM
Hagstrom
J E
JE
Wolff
J A
JA
Budker
V G
VG
D004270:DNA, Circular / Q000737:chemistry*; D005453:Fluorescence; D011107:Polylysine / Q000494:pharmacology
D004270:DNA, Circular; D011107:Polylysine