Molecular cloning of mouse Lrp7(Lr3) cDNA and chromosomal mapping of orthologous genes in mouse and human.
LRP7 (the HGMW-approved gene symbol for LR3) is a novel member of the low-density lipoprotein receptor (LDLR) family. The unique modular features in both its extracellular and its intracellular regions and functional analysis suggest it to be a multifunctional protein with mitogenic activity and potential ligand-induced signal transduction capability. We describe here the cloning of Lrp7, the orthologue of the human LRP7 gene, from a mouse liver cDNA library and its expression profile in mouse tissues and developmental stages. Lrp7 is highly homologous to the human sequence, with 95% identity at the amino acid level and a perfect conservation of all the recognizable functional domains. A 6-kb mRNA transcript could be detected in all the tissues examined, with the highest expression levels in liver, heart, and lung and the lowest levels in brain and spleen. A similar expression level of a smaller transcript (4.5 kb) was seen at the four embryonic stages examined. We mapped the Lrp 7 gene to mouse chromosome 19B and the LRP7 gene to human chromosome 11q13.4. The LRP7 gene was also localized by radiation hybrid mapping between markers D11S24270 and D11S1975.
Chen
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Bayer Research Center, Pharmaceutical Division, Bayer Corporation, West Haven, Connecticut 06516-4175, USA. david.chen.b@bayer.com
Lathrop
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Dong
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D016475:3T3 Cells; D000595:Amino Acid Sequence; D000818:Animals; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics; D005865:Gestational Age; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D026502:LDL-Receptor Related Proteins; D008099:Liver / Q000378:metabolism; D060492:Low Density Lipoprotein Receptor-Related Protein-5; D008677:Metaphase; D051379:Mice; D008954:Models, Biological; D008969:Molecular Sequence Data; D020161:Physical Chromosome Mapping; D011973:Receptors, LDL / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution
D026502:LDL-Receptor Related Proteins; C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5; C555483:Lrp5 protein, mouse; D011973:Receptors, LDL