Co-localization of interleukin-1 receptor type I and interleukin-1 receptor antagonist with vasopressin in magnocellular neurons of the paraventricular and supraoptic nuclei of the rat hypothalamus.
Interleukin-1 receptor type I and interleukin-1 receptor antagonist were found in magnocellular neurons of the paraventricular and supraoptic nuclei of the rat hypothalamus by immunohistochemical detection. Double-labelling experiments revealed that both proteins occurred in vasopressin-containing neurons. A similar distribution pattern was observed in a group of vasopressin-positive accessory magnocellular neurons. Axons emanating from the interleukin-1 receptor type I- and interleukin-1 receptor antagonist-immunoreactive neuronal cell bodies could be seen within the hypothalamic nuclei, and varicosities expressing interleukin-1 receptor antagonist immunoreactivity were observed in the internal zone of the median eminence, as well as in the hypothalamo-pituitary projection. The co-localization of interleukin-1 receptor type I with vasopressin is in agreement with findings that interleukin-1 has a stimulatory effect on vasopressin synthesis and release. The hypothalamic neurons may serve as a source of interleukin-1 receptor antagonist to balance the effects of interleukin-1.
Department of Clinical Neuroscience & Family Medicine, Karolinska Institute, Huddinge University Hospital, Sweden.
A
A
Diana
A M
AM
Van Dam
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B
Winblad
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M
Schultzberg
D000818:Animals; D000911:Antibodies, Monoclonal; D015153:Blotting, Western; D003346:Corticotropin-Releasing Hormone / Q000502:physiology; D005455:Fluorescent Antibody Technique; D007124:Immunoenzyme Techniques; D008297:Male; D009474:Neurons / Q000737:chemistry / Q000502:physiology*; D010286:Paraventricular Hypothalamic Nucleus / Q000737:chemistry / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017472:Receptors, Interleukin-1 / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D053573:Receptors, Interleukin-1 Type I; D013495:Supraoptic Nucleus / Q000737:chemistry / Q000166:cytology*; D014667:Vasopressins / Q000502:physiology*
D000911:Antibodies, Monoclonal; D017472:Receptors, Interleukin-1; D053573:Receptors, Interleukin-1 Type I; D014667:Vasopressins; D003346:Corticotropin-Releasing Hormone