A simple hydroponic culture method for the development of a highly viable root system in Arabidopsis thaliana.
In the studies of nutritional absorption and metal toxicity in the root, it is important to grow plants without technical damage. We established a simple hydroponic culture system for Arabidopsis thaliana to obtain a healthy plant having a well-developed root system with many lateral roots. The phytotoxic effects of Cr, Cu, and Al ions were examined by FDA-PI staining using this culture system. The pattern of root inhibition varied with the ion, suggesting the usefulness of this culture system.
Toda
T
T
Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan. tomomi@cc.gifu-u.ac.jp
Koyama
H
H
Hara
T
T
D000535:Aluminum / Q000633:toxicity; D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development*; D002857:Chromium / Q000633:toxicity; D003300:Copper / Q000633:toxicity; D018527:Hydroponics / Q000379:methods*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D013194:Staining and Labeling
D002857:Chromium; D003300:Copper; D000535:Aluminum