Regulation of PiT-1, a sodium-dependent phosphate co-transporter in rat parathyroid glands.
A cDNA encoding an Na+-Pi co-transporter, termed rat PiT-1, has now been isolated from rat parathyroid. Expression of rat PiT-1 in Xenopus oocytes revealed that it possesses Na+-dependent Pi co-transport activity. The amount of PiT-1 mRNA in the parathyroid of vitamin D-deficient rats was reduced compared with that in normal animals, and increased markedly after administration of 1,25-dihydroxyvitamin D3. Furthermore, the abundance of PiT-1 mRNA in the parathyroid was much greater in rats fed a low-Pi diet than in those fed a high-Pi diet. Thus, rat PiT-1 may contribute to the effects of Pi and vitamin D on parathyroid function.
Department of Clinical Nutrition, School of Medicine, Tokushima University, Tokushima City, Japan.
K
K
Miyamoto
S
S
Tatsumi
H
H
Segawa
K
K
Morita
T
T
Nii
A
A
Fujioka
M
M
Kitano
Y
Y
Inoue
E
E
Takeda
D000818:Animals; D002117:Calcitriol / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D004032:Diet; D010280:Parathyroid Glands / Q000378:metabolism*; D010710:Phosphates / Q000008:administration & dosage / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters; D013961:Thyroid Gland / Q000378:metabolism; D014808:Vitamin D Deficiency / Q000378:metabolism
D002352:Carrier Proteins; D010710:Phosphates; D012333:RNA, Messenger; C493588:Slc20a1 protein, rat; D050608:Sodium-Phosphate Cotransporter Proteins; D050611:Sodium-Phosphate Cotransporter Proteins, Type III; D027981:Symporters; D002117:Calcitriol