Interferon-induced human MxA GTPase blocks nuclear import of Thogoto virus nucleocapsids.
Interferon-induced human MxA protein belongs to the dynamin superfamily of large GTPases. It exhibits antiviral activity against a variety of RNA viruses, including Thogoto virus, an influenza virus-like orthomyxovirus transmitted by ticks. Here, we report that MxA blocks the transport of Thogoto virus nucleocapsids into the nucleus, thereby preventing transcription of the viral genome. This interaction can be abolished by a mAb that neutralizes the antiviral activity of MxA. Our results reveal an antiviral mechanism whereby an interferon-induced protein traps the incoming virus and interferes with proper transport of the viral genome to its ultimate target compartment within the infected cell.
Kochs
G
G
Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, D-79008 Freiburg, Germany.
Haller
O
O
D016475:3T3 Cells; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000502:physiology* / Q000821:virology*; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D019204:GTP-Binding Proteins; D016679:Genome, Viral; D006801:Humans; D007372:Interferons / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D008954:Models, Biological; D064547:Myxovirus Resistance Proteins; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism*; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D018117:Thogotovirus / Q000235:genetics / Q000502:physiology*; D014158:Transcription, Genetic; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D000998:Antiviral Agents; C579071:MX1 protein, human; D064547:Myxovirus Resistance Proteins; D019590:Nucleocapsid Proteins; D011506:Proteins; D007372:Interferons; D003513:Cycloheximide; D020558:GTP Phosphohydrolases; D019204:GTP-Binding Proteins