Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination.
A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.
Institute of Genetic Ecology, Tohoku University, Sendai, Japan.
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Takanami
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Sato
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Ishihara
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Katsura
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Takahashi
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Higashitani
D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D016208:Databases, Factual; D006066:Gonads / Q000166:cytology / Q000502:physiology; D008540:Meiosis / Q000235:genetics; D008969:Molecular Sequence Data; D009866:Oogenesis / Q000502:physiology; D010063:Ovum / Q000166:cytology / Q000502:physiology; D010802:Phylogeny; D051135:Rad51 Recombinase; D011938:Rec A Recombinases / Q000138:chemical synthesis / Q000235:genetics*; D011995:Recombination, Genetic
D029742:Caenorhabditis elegans Proteins; D051135:Rad51 Recombinase; D011938:Rec A Recombinases; C495148:rad-51 protein, C elegans