The specific genes for lantibiotic mutacin II biosynthesis in Streptococcus mutans T8 are clustered and can be transferred en bloc.
Mutacin II is a ribosomally synthesized peptide lantibiotic produced by group II Streptococcus mutans. DNA sequencing has revealed that the mutacin II biosynthetic gene cluster consists of seven specific open reading frames: a regulator (mutR), the prepromutacin structural gene (mutA), a modifying protein (mutM), an ABC transporter (mutT), and an immunity cluster (mutFEG). Transformations of a non-mutacin-producing strain, S. mutans UA159, and a mutacin I-producing strain, S. mutans UA140, with chromosomal DNA from S. mutans T8 with an aphIII marker inserted upstream of the mutacin II structural gene yielded transformants producing mutacin II and mutacins I and II, respectively.
Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
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Novak
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Caufield
D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics* / Q000494:pharmacology; D005798:Genes, Bacterial; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D005810:Multigene Family; D013211:Staphylococcus aureus / Q000187:drug effects; D013295:Streptococcus mutans / Q000235:genetics / Q000378:metabolism*; D016986:Streptococcus sobrinus / Q000187:drug effects / Q000235:genetics; D014169:Transformation, Bacterial
D001430:Bacteriocins; C069066:mutacin II