Evaluation of immunomagnetic separation and plating media for recovery of Salmonella from meat.
Immunomagnetic separation (IMS) was compared with selective enrichment in selenite cystine (SC) broth for isolation of Salmonella from 86 artificially contaminated ground beef samples. Both Rambach agar (RA) and Hektoen enteric (HE) agar were used as selective plating media. The highest count of Salmonella colonies per plate was obtained after enrichment in SC broth and plating on RA (mean value: 111.1+/-58.1 CFU per plate); the lowest count was obtained after IMS and plating on HE agar (mean value: 65.4+/-36.6 CFU per plate). Salmonella in preenrichment was concentrated 1.7-fold by IMS and represented 31% of the microbial population captured by the beads, but nonspecific binding was high. As a result of the large numbers of competing bacteria, isolations on both RA and HE agar following IMS were quite difficult (mean value for Salmonella colonies: 79.9+/-42.7 CFU per plate; mean value for non-Salmonella colonies: 144.1+/-75.7 CFU per plate; ratio of Salmonella to non-Salmonella colonies: 0.8). This study indicates that SC broth is superior to IMS in the isolation of Salmonella from raw ground meat.
Cattedra di Igiene, Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Università del Molise, Campobasso, Italy.
G
G
Ripabelli
M L
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Sammarco
G M
GM
Grasso
D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D015169:Colony Count, Microbial; D003470:Culture Media; D005069:Evaluation Studies as Topic; D018189:Immunomagnetic Separation / Q000379:methods*; D008461:Meat Products / Q000382:microbiology*; D012475:Salmonella / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media