Platelet high affinity low density lipoprotein binding and import of lipoprotein derived phospholipids.
The binding of low density lipoprotein (LDL) to the platelet cell membrane could facilitate the transfer of phospholipids from LDL to the platelets. A polyclonal antibody against the platelet glycoproteins IIb/IIIa inhibited the high affinity binding of 125I-LDL by up to 80%. The transfer of pyrene (py)-labeled sphingomyelin (SM), phosphatidylcholine and phosphatidylethanolamine from LDL to the platelets was unaffected by the antibody. The lectin wheat germ agglutinin (WGA) reduced the binding of 125I-LDL to the platelets by approximately 80%. In contrast, the lectin stimulated the transfer of SM from LDL into the platelets by about three-fold. WGA also specifically augmented the transfer of py-SM between lipid vesicles and the platelets, the stimulation being abolished in the presence of N-acetylglucosamine. Dextran sulfate (DS) increased the specific binding of 125I-LDL to the platelets by up to 2.8-fold. On the other hand, the import of LDL-derived py-phospholipids was unaffected by DS. Together, the results indicate that the phospholipid transfer from LDL to the platelets is independent of the high affinity LDL binding to the platelets and is specifically stimulated by WGA. Thus, the interactions of platelets with LDL phospholipids differ markedly from those with the apoprotein components of the lipoproteins.
Physiologisches Institut der Universität München, Munich, Germany.
P
P
Dobner
E
E
Koller
B
B
Engelmann
D000117:Acetylglucosamine / Q000494:pharmacology; D000373:Agglutinins / Q000378:metabolism; D000906:Antibodies / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D016264:Dextran Sulfate / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism; D010713:Phosphatidylcholines / Q000493:pharmacokinetics; D010714:Phosphatidylethanolamines / Q000493:pharmacokinetics; D010743:Phospholipids / Q000493:pharmacokinetics*; D019039:Platelet Glycoprotein GPIIb-IIIa Complex / Q000378:metabolism*; D011485:Protein Binding; D011721:Pyrenes / Q000378:metabolism; D013109:Sphingomyelins / Q000493:pharmacokinetics
D000373:Agglutinins; D000906:Antibodies; D005456:Fluorescent Dyes; D037102:Lectins; D008077:Lipoproteins, LDL; D008562:Membrane Glycoproteins; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D019039:Platelet Glycoprotein GPIIb-IIIa Complex; D011721:Pyrenes; D013109:Sphingomyelins; D016264:Dextran Sulfate; C030984:pyrene; D000117:Acetylglucosamine