Influence of organochlorine pesticides on ATPase activities of microsomal fractions of bovine oviductal and endometrial cells.
In the present study the effects of organochlorine pesticides: o,p'-DDT, p,p''-DDT, methoxychlor, and lindane on ATPase activities of microsomal fractions of bovine oviductal and endometrial cells were investigated. We were not able to characterize a Ca2+ stimulated ATPase, whereas 57 and 15% of the total ATPase activity were sensitive to Mg2+ in the oviductal and endometrial fractions, respectively. After 10 min preincubation with the four organochlorines, a significant inhibition was found only with o,p''-DDT at 32 microM (27.9%) and 64 microM (35.6%) in the oviductal microsomal fraction and at 64 microM (32.2%) in that of the endometrium. Increasing the preincubation time to 30 min, the Mg2+ ATPase in the endometrial fraction was significantly inhibited by all four pesticides at 64 microM, but in the oviductal fraction only at 64 microM o,p''-DDT. It is suggested that organochlorine pesticides can have an influence on cells responsible for reproduction.
Research Institute for the Biology of Farm Animals, Dummerstorf, Germany. tiemann@fbn-dummerstorf.de
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Tiemann
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Küchenmeister
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002417:Cattle; D002478:Cells, Cultured; D004717:Endometrium / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D005187:Fallopian Tubes / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D005260:Female; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000633:toxicity*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*
D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D000251:Adenosine Triphosphatases; D017301:Ca(2+) Mg(2+)-ATPase; D000252:Calcium-Transporting ATPases; D002118:Calcium