Duplication of insertion element IS50 associated with Tn5 transposition in Azospirillum brasilense.
The characterization of a DNA fragment with a Tn5 insertion in a regulatory nif gene of Azospirillum brasilense is reported. Restriction endonuclease mapping, Southern hybridization with a Tn5 probe, and nucleotide sequencing revealed that IS50 had duplicated in Tn5. The duplication of an IS50 element suggests the occurrence of a replicative mechanism of transposition. A strategy, based on the bacterial ability of homologous recombination that was used to precisely eliminate Tn5 along with the duplicated IS50 element, is presented.
Tripathi
A K
AK
Laboratory of Bacterial Genetics, School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India. tripathianil@hotmail.com
Tripathi
R
R
Ganguli
A
A
Bazzicalupo
M
M
D015806:Azospirillum brasilense / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D004269:DNA, Bacterial