A novel plasmid recombination mechanism of the marine cyanobacterium Synechococcus sp. PCC7002.
We describe a novel mechanism of site-specific recombination in the unicellular marine cyanobacterium Synechococcus sp. PCC7002. The specific recombination sites on the smallest plasmid pAQ1 were localized by studying the properties of pAQ1-derived shuttle-vectors. We found that a palindromic element, the core sequence of which is G(G/A)CGATCGCC, functions as a resolution site for site-specific plasmid recombination. Furthermore, site-directed mutagenesis analysis of the element show that the site-specific recombination in the cyanobacterium requires sequence specificity, symmetry in the core sequence and, in part, the spacing between the elements. Interestingly, this element is over-represented not only in pAQ1 and in the genome of the cyanobacterium, but also in the accumulated cyanobacterial sequences from Synechococcus sp. PCC6301, PCC7942, vulcanus and Synechocystis sp. PCC6803 within GenBank and EMBL databases. Thus, these findings strongly suggest that the site-specific recombination mechanism based on the palindromic element should be common in these cyanobacteria.
Biological Sciences Department, Toray Research Center, Inc., Kamakura, Kanagawa, Japan. Hideo_Akiyama@trc.toray.co.jp
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Akiyama
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Kanai
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Hirano
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Miyasaka
D000458:Cyanobacteria / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005822:Genetic Vectors; D008957:Models, Genetic; D010957:Plasmids / Q000235:genetics*; D021382:Protein Sorting Signals / Q000032:analysis; D011995:Recombination, Genetic; D014170:Transformation, Genetic
D021382:Protein Sorting Signals