Alginate beads as an affinity material for alpha amylases.
Calcium-alginate beads were found to bind a variety of enzymes in a nonspecific fashion. However, alpha amylases from porcine pancreas, Bacillus subtilis (BAN 240L) and wheat germ bound at a significant level and B. subtilis and wheat germ amylases could be eluted with 1M maltose. The wheat germ alpha amylase could be purified 45 fold with 70% recovery. The SDS-PAGE pattern showed significant purification by this single step strategy.
Chemistry Department, Indian Institute of Technology, Delhi, India.
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D000464:Alginates; D000818:Animals; D001412:Bacillus subtilis / Q000201:enzymology; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D006863:Hydrogen-Ion Concentration; D010179:Pancreas / Q000201:enzymology; D013552:Swine; D014908:Triticum / Q000201:enzymology; D000516:alpha-Amylases / Q000302:isolation & purification*
D000464:Alginates; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D000516:alpha-Amylases