Biphasic insulin-releasing effect of BTS 67 582 in rats.
BTS 67 582 (1,1-dimethyl-2(2-morpholinophenyl)guanidine fumarate) is being developed as a short-acting anti-diabetic insulin secretagogue. The effect of BTS 67 582 on the phasic pattern of insulin release was assessed in anaesthetized normal rats by measuring arterial plasma insulin concentrations while arterial glucose concentrations were fixed at 6, 8.5 and 12.5 mM. Intravenous BTS 67 582 (10 mg kg(-1)) induced an immediate but transient increase in insulin concentrations which declined by 10 min (first phase). This was followed by a smaller but sustained increase in insulin concentrations (second phase). The increment from basal to peak insulin release (0-2 min) was independent of glucose, but the first phase was maintained for longer and the second phase was greater at the highest concentration of glucose (12.5 mM). BTS 67 582 also extended the first-phase insulin response to a standard intravenous glucose challenge and enhanced the rate of glucose disappearance by approximately 12%. Thus BTS 67 582 causes biphasic stimulation of insulin release and augments the insulin-releasing effect of glucose.
Department of Pharmaceutical and Biological Sciences, Aston University, Birmingham, UK.
D A
DA
Storey
C J
CJ
Bailey
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D006146:Guanidines / Q000494:pharmacology*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007275:Injections, Intravenous; D007328:Insulin / Q000097:blood* / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar
D001786:Blood Glucose; D006146:Guanidines; D007004:Hypoglycemic Agents; D007328:Insulin; C092674:BTS 67582