Structural identification of sulfated tyrosine in human urine.
A reliable HPLC method was used for the identification of positional isomerism and stereoisomerism of sulfated tyrosine residues in human urine. Upon separation of human urine by ion-pair HPLC on a reverse-phase column, p-tyrosine-O-sulfate (p-TyrS) was identified. Differentiation of the L and D forms was done by using a column with a chiral stationary phase. It was concluded that L-p-tyrosine (L-p-Tyr) which is the predominant tyrosine isomer in the human body, was sulfated and excreted in human urine as a normal constituent. The sulfated forms of D-p-Tyr and m-Tyr could not be detected under these analytical conditions.
Department of Biological Resource Sciences, Miyazaki University, Japan.
J G
JG
Ranasinghe
Y
Y
Sakakibara
M
M
Harada
K
K
Nishiyama
M C
MC
Liu
M
M
Suiko
D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007536:Isomerism; D013237:Stereoisomerism; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000737:chemistry / Q000652:urine
C005662:tyrosine O-sulfate; D014443:Tyrosine