Enhanced resistance to bacterial diseases of transgenic tobacco plants overexpressing sarcotoxin IA, a bactericidal peptide of insect.
Sarcotoxin IA is a bactericidal peptide of 39 amino acids found in the common flesh fly, Sarcophaga peregrina. Many agronomically important bacteria in Japan are killed by this peptide at sub-micro molar levels, and the growth of tobacco and rice suspension cultured cells is not inhibited with less than 25 microM. Transgenic tobacco plants which overexpress the peptide, i.e. over 250 pmol per gram of fresh leaf, under the control of a high expression constitutive promoter showed enhanced resistance to the pathogens for wild fire disease (Pseudomonas syringae pv. tabaci) and bacterial soft rot disease (Erwinia carotovora subsp. carotovora).
National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305-8602, Japan.
M
M
Ohshima
I
I
Mitsuhara
M
M
Okamoto
S
S
Sawano
K
K
Nishiyama
H
H
Kaku
S
S
Natori
Y
Y
Ohashi
D000818:Animals; D000900:Anti-Bacterial Agents; D004885:Erwinia; D018506:Gene Expression Regulation, Plant; D018014:Gene Transfer Techniques; D019476:Insect Proteins / Q000096:biosynthesis / Q000235:genetics*; D007313:Insecta; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000382:microbiology; D010947:Plants, Toxic; D011549:Pseudomonas; D014026:Nicotiana / Q000235:genetics* / Q000382:microbiology*
D000900:Anti-Bacterial Agents; D019476:Insect Proteins; C054733:sarcotoxin IA protein, Sarcophaga peregrina