Sheep poxvirus identification by PCR in cell cultures.
A simple, rapid and specific diagnostic polymerase chain reaction (PCR) method was developed for sheep poxvirus identification. The primers used were from the sequenced genomes of the capripox viruses KS-1 and InS-1. Six different sheep pox isolates were tested against two orf (parapox) and three animal herpesviruses as controls. Material from uninfected cell cultures was also used as control. The sensitivity of the PCR was approximately equivalent with each of the two primers and for the six sheep pox isolates. All the negative control virus DNAs were negative and differed clearly from those of the sheep pox strains.
Centre of Athens Veterinary Institutions, Institute of Infectious and Parasitic Diseases, Attiki, Greece.
O
O
Mangana-Vougiouka
P
P
Markoulatos
G
G
Koptopoulos
K
K
Nomikou
N
N
Bakandritsos
O
O
Papadopoulos
D000818:Animals; D018929:Cell Culture Techniques; D004279:DNA, Viral / Q000032:analysis; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011212:Poxviridae / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D011213:Poxviridae Infections / Q000662:veterinary; D012680:Sensitivity and Specificity; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology; D013737:Testis / Q000166:cytology
D004279:DNA, Viral