Asymmetric distribution of phosphatidylethanolamine in C. albicans: possible mediation by CDR1, a multidrug transporter belonging to ATP binding cassette (ABC) superfamily.
By using two molecular probes, we demonstrate that only 4% of total phosphatidylethanolamine (PtdEtn) in the plasma membrane (PM) of a human pathogenic yeast, Candida albicans, is present in its external half. Evidence is presented to show that the availability of PtdEtn could be related to the expression of a multidrug transporter CDR1 of C. albicans, and the process is energy-dependent. A homozygous CDR1 disruptant strain of C. albicans shows almost 23% reduction in the external labelling of PtdEtn. This report shows that, similar to human MDRs, yeast multidrug transporter could also be involved in aminophospholipid translocation.
Membrane Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
S
S
Dogra
S
S
Krishnamurthy
V
V
Gupta
B L
BL
Dixit
C M
CM
Gupta
D
D
Sanglard
R
R
Prasad
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D002176:Candida albicans / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D018432:Drug Resistance, Multiple; D005450:Fluorescamine / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism*; D008563:Membrane Lipids / Q000032:analysis / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D026901:Membrane Transport Proteins; D010714:Phosphatidylethanolamines / Q000032:analysis* / Q000378:metabolism; D014302:Trinitrobenzenesulfonic Acid / Q000378:metabolism
D018528:ATP-Binding Cassette Transporters; C094693:CDR1 protein, Candida albicans; D005656:Fungal Proteins; D008563:Membrane Lipids; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D010714:Phosphatidylethanolamines; D005450:Fluorescamine; D000255:Adenosine Triphosphate; D014302:Trinitrobenzenesulfonic Acid