Biological effects of man-made mineral fibers (I)--Reactive oxygen species production and calcium homeostasis in alveolar macrophages.
10 types of standard mineral fiber samples (JFM fibers) were tested for their cytotoxicity in alveolar macrophages (AM) in vitro experiments, in which UICC chrysotile B was used as a positive control. The cytotoxicity included the production of superoxide anion radical and hydrogen peroxide, depletion of glutathione (GSH) and increase of intracellular free calcium. The results showed that chrysotile and most of the 10 mineral fibers could increase the production of superoxide anion and hydrogen peroxide, deplete the concentration of GSH and increase the level of free intracellular Ca2+ in AM. But all the effects of JFM fibers were lower than that induced by UICC chrysotile B. Although the cytotoxicity of JFM fibers were lower than that of asbestos, these mineral fibers should be used with highly care for workers in industries.
Department of Occupational Health, Beijing Medical University, P.R. China.
Q E
QE
Wang
C H
CH
Han
W D
WD
Wu
H B
HB
Wang
S J
SJ
Liu
N
N
Kohyama
D000818:Animals; D002118:Calcium / Q000378:metabolism / Q000493:pharmacokinetics; D005978:Glutathione / Q000378:metabolism; D006168:Guinea Pigs; D006861:Hydrogen Peroxide / Q000378:metabolism; D016676:Macrophages, Alveolar / Q000187:drug effects* / Q000276:immunology; D019016:Mineral Fibers / Q000009:adverse effects*; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000378:metabolism
D019016:Mineral Fibers; D017382:Reactive Oxygen Species; D013481:Superoxides; D006861:Hydrogen Peroxide; D005978:Glutathione; D002118:Calcium