Analysis of the multimeric state of proteins by matrix assisted laser desorption/ionization mass spectrometry after cross-linking with glutaraldehyde.
We have undertaken a systematic study on the suitability of matrix-assisted laser desorption/ionization mass spectrometry to analyze and determine the multimericity of several proteins after cross-linking with glutaraldehyde. Using both commercially available proteins and others of viral origin currently being characterized in our laboratory, we studied the range of concentrations of cross-linker and protein for optimal analysis. Under the conditions developed during this study, we confirmed the multimeric states of three phage PRD1 structural proteins with monomeric masses ranging from 13.5 to 63 kDa. In addition, we addressed the question of the general applicability of the method by using it successfully to confirm the stoichiometry of the heptameric chaperonin GroEL, a bacterial protein with a mass well over 450 kDa.
Helin
J
J
Institute of Biotechnology, University of Helsinki, Finland.
Caldentey
J
J
Kalkkinen
N
N
Bamford
D H
DH
D000818:Animals; D003432:Cross-Linking Reagents; D019281:Dimerization; D005976:Glutaral; D011506:Proteins / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014764:Viral Proteins / Q000737:chemistry*
D003432:Cross-Linking Reagents; D011506:Proteins; D014764:Viral Proteins; D005976:Glutaral