In vitro and in vivo study of the clastogenicity of the flavone cirsitakaoside extracted from Scoparia dulcis L. (Scrophulariaceae).
The mutagenic effect of the flavone cirsitakaoside extracted from the medicinal herb Scoparia dulcis was evaluated in vitro by using human peripheral blood cultures treated with doses of 5, 10, and 15 microg of the flavone/ml culture medium for 48 h. The compound proved to be mutagenic at the highest concentration tested (15 microg/ml). Furthermore, the proliferative index was significantly reduced in all cultures treated with the flavone, although the mitotic index was not reduced. However, the clastogenic activity of the flavone cirsitakaoside was not observed when Swiss mice were treated orally with doses of 10, 20, and 30 mg/animal for 24 h.
Pereira-Martins
S R
SR
Department of Biology, Federal University of Maranhão, São Luís, MA. Brazil. smartins@rgm.fmrp.usp.br
Takahashi
C S
CS
Tavares
D C
DC
Torres
L M
LM
D000328:Adult; D000818:Animals; D002869:Chromosome Aberrations; D003520:Cyclophosphamide / Q000032:analysis; D005260:Female; D047309:Flavones; D005419:Flavonoids / Q000633:toxicity*; D006027:Glycosides / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000648:ultrastructure; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*
D047309:Flavones; D005419:Flavonoids; D006027:Glycosides; D009153:Mutagens; C107783:cirsimarin; D003520:Cyclophosphamide