Moisture effects on protein-excipient interactions in spray-dried powders. Nature of destabilizing effects of sucrose.
The preparation of stable solid protein formulations presents significant challenges. Ultimately, the interactions between incorporated excipients and the pharmaceutical protein determine the formulation stability. In this study, moisture was utilized to probe the interactions between a model protein, trypsinogen, and sucrose in the solid state, following spray drying. Through investigation of the physical properties of the spray-dried formulations, we attempted to elucidate the mechanisms underlying the previously observed stabilizing and destabilizing effects of the carbohydrate during spray drying. Both dynamic and equilibrium moisture uptake studies indicated the presence of an optimal protein-sugar hydrogen bonding network. At low sucrose contents, a preferential protein-sucrose hydrogen bonding interaction was dominant, resulting in protein stabilization. However, at high carbohydrate concentrations, preferential sugar-sugar interactions prevailed, resulting in a phase separation within the formulation matrix. The preferential incorporation of the sucrose molecules in a sugar-rich phase reduced the actual amount of the carbohydrate available to interact with the protein and thereby decreased the number of effective protein-sucrose contacts. As a consequence, the protein could not be effectively protected during spray drying. We hypothesize that the observed phase separation at this sucrose concentration regime originates from its exclusion from the protein in solution before spray drying, further accompanied by preferential clustering of the sucrose molecules.
Biopharmaceutical Research & Development, ALZA Corporation, 950 Page Mill Road, Palo Alto, California 94303, USA. stelios.tzannis@inhale.com
S T
ST
Tzannis
S J
SJ
Prestrelski
D002152:Calorimetry, Differential Scanning; D002626:Chemistry, Pharmaceutical; D004347:Drug Interactions; D005079:Excipients / Q000737:chemistry*; D010316:Particle Size; D011208:Powders; D013395:Sucrose / Q000737:chemistry*; D014362:Trypsinogen / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D005079:Excipients; D011208:Powders; D014867:Water; D013395:Sucrose; D014362:Trypsinogen