A new sugar chain of the proteinase inhibitor from latex of Carica papaya.
The structure of a sugar chain of the proteinase inhibitor from the latex of Carica papaya was studied. Sugar chains liberated on hydrazinolysis were N-acetylated, and their reducing-end residues were tagged with 2-aminopyridine. One major sugar chain was detected on size-fractionation and reversed-phase HPLC analyses. The structure of the PA-sugar chain was determined by two-dimensional sugar mapping combined with sequential exoglycosidase digestion and partial acid hydrolysis, and by 750 MHz 1H-NMR spectroscopy. The structure found was Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3) (Xylbeta1-2)Manbeta1- 4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc. This sugar chain represents a new plant-type sugar chain with five mannose residues.
Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan.
A
A
Shimazaki
Y
Y
Makino
K
K
Omichi
S
S
Odani
S
S
Hase
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis / Q000737:chemistry*; D007840:Latex / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D010944:Plants; D011480:Protease Inhibitors / Q000737:chemistry* / Q000378:metabolism
D002241:Carbohydrates; D007840:Latex; D011480:Protease Inhibitors