Apolipoprotein A-I of hyperlipidemia atherosclerosis prone (LAP) quail: cDNA sequence and tissue expression.
Apolipoprotein A-I (apo A-I) has an important role in the transport of cholesterol. This study describes the complete nucleotide and deduced amino acid sequence for apo A-I of LAP quail. A full length apo A-I cDNA clone for hyperlipidemia atherosclerosis prone (LAP) quail was isolated from a lambda gt10 liver cDNA library. The DNA sequence of LAP apo A-I cDNA was similar to that of normal Japanese quail. The deduced amino acid sequence of LAP apo A-I was hence identical to that of normal Japanese quail. LAP apo A-I mRNA is about 1.4 kilobases in length and expressed in a variety of tissues including small intestine, liver, lung, breast muscle, testis, and heart. Although the tissue distribution of apo A-I was similar between strains, LAP quail expressed more apo A-I mRNA than normal Japanese quail in all tissues examined. This tendency was pronounced with the small intestine. Although the concentration of serum apo A-I did not correlate with the tissue expression of mRNA, the observation may suggest that the increased apo A-I expression in LAP strain had some relevance to the susceptibility of this strain to the experimental atherosclerosis.
Laboratory of Applied Biochemistry, Faculty of Agriculture, University of The Ryukyus, Okinawa, Japan.
H
H
Iwasaki
H
H
Oku
T
T
Toda
T
T
Nasu
K
K
Oda
T
T
Miyagi
I
I
Chinen
D000595:Amino Acid Sequence; D000818:Animals; D016632:Apolipoprotein A-I / Q000235:genetics*; D001161:Arteriosclerosis / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003370:Coturnix; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D006949:Hyperlipidemias / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D014018:Tissue Distribution
D016632:Apolipoprotein A-I; D018076:DNA, Complementary; D012333:RNA, Messenger