Maturation site of dengue type 2 virus in cultured mosquito C6/36 cells and Vero cells.
The maturation of dengue virus (type 2, New Guinea B strain; abbreviated Den-2) in cultured mosquito C3/36 cells and Vero cells was studied by immunoelectron microscopy for the first 7 days after cells were infected, assays of the virus were done by the peroxidase-antiperoxidase method. Virus titers in both kinds of cells were highest on day 6. These cells were then observed with an electron microscope. Den-2 was round and measured about 50 nm in diameter. The virus matured mainly at the membranes of cytoplasmic vacuoles and vesicles, but a few budding viral particles were seen in the cell surface membrane. Immunogold labelling with rabbit antiserum against the E protein of Japanese encephalitis virus was used to locate specific antigens of the Den-2 envelope protein. The results showed that the E protein was in vacuoles, vesicles, and endoplasmic reticulum of the cells. In this study there were no differences between C6/36 cells and Vero cells infected with Den-2.
Department of Medical Zoology, Kobe University School of Medicine, Japan.
S
S
Rahman
T
T
Matsumura
K
K
Masuda
K
K
Kanemura
T
T
Fukunaga
D000818:Animals; D002460:Cell Line / Q000821:virology; D002522:Chlorocebus aethiops; D009033:Culicidae / Q000166:cytology / Q000648:ultrastructure / Q000821:virology; D003593:Cytoplasm / Q000648:ultrastructure / Q000821:virology; D003716:Dengue Virus / Q000254:growth & development*; D016253:Microscopy, Immunoelectron; D011817:Rabbits; D014709:Vero Cells / Q000648:ultrastructure / Q000821:virology