Effect of manganese on the concentration of amino acids in different regions of the rat brain.
The present study was designed to determine if chronic exposure of weanlings and adult rats to Mn produces significant alterations in amino acid concentrations in different regions of the rat brain. Weanling (30 day old) and adult (90 day old) male rats were exposed to 10 and 20 mg Mn/kg body weight per day, by gavage, for 30 days. Forty-eight hours after the last dose, animals were sacrificed by decapitation and brains were dissected into different regions to determine the concentration of amino acids by HPLC/EC. A dose dependent decrease in body weight gain was found in the adult, but not in the weanling rats. Significant increases occurred in concentrations of aspartate, glutamate, glutamine, taurine and gamma-aminobutyric acid (GABA) in the cerebellum of the adult rats dosed with 20 mg/kg per day, Mn. A significant decrease in the concentration of glutamine was observed in caudate nucleus and hippocampus of weanling rats dosed with 10 mg/kg, Mn. These data suggest that chronic Mn exposure can produce a decrease in body weight gain in adult rats and alterations in amino acids in different regions of weanling and adult rat brains.
Lipe
G W
GW
Neurochemistry Laboratory, FDA, Jefferson, AR 72079, USA.
Duhart
H
H
Newport
G D
GD
Slikker
W
W
Ali
S F
SF
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008297:Male; D020149:Manganese Poisoning; D051381:Rats; D015430:Weight Gain / Q000187:drug effects
D000596:Amino Acids