Stability of phosphorothioate oligonucleotides in aqueous ammonia in presence of stainless steel.
Oligodeoxyribonucleotide phosphorothioates undergo desulfurization in presence of metal in aqueous ammonia at elevated temperatures. The extent of sulfur loss and chain cleavage have been investigated.
Isis Pharmaceuticals, Carlsbad, CA 92008, USA.
B J
BJ
Turney
Z S
ZS
Cheruvallath
M
M
Andrade
D L
DL
Cole
V T
VT
Ravikumar
D000641:Ammonia; D001483:Base Sequence; D004355:Drug Stability; D005898:Glass; D019906:Nuclear Magnetic Resonance, Biomolecular; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D013193:Stainless Steel; D013873:Thionucleotides / Q000737:chemistry*; D014867:Water
D009838:Oligodeoxyribonucleotides; D013873:Thionucleotides; D014867:Water; D013193:Stainless Steel; D000641:Ammonia