Stabilization of the intermediate in frameshift mutation.
A mismatch repair, proofreading deficient mutant of Escherichia coli lost a C from a C8 run at a rate 10 times higher than the loss of A from an A8 sequence in the same double mutant. This greater frameshift instability of a homopolymeric run of C's may be due to stabilization of a stacked intermediate. Gain of a (CA) unit in a similarly constructed (CA)15 sequence occurred at a rate about 1/3 that previously reported for a (CA)14 construct losing a (CA) repeat unit.
Department of Molecular Genetics and Cell Biology, The University of Chicago, 920 E. 58th Street, Chicago, IL 60637, USA.
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D
Sagher
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Hsu
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Strauss
D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D016368:Frameshift Mutation / Q000235:genetics*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012091:Repetitive Sequences, Nucleic Acid; D013698:Templates, Genetic
D004269:DNA, Bacterial