Denaturation of ovalbumin abrogates oral induction of airway hyperreactivity and IgG1, IgG2 antibody responses in guinea pigs.
BACKGROUND
The effects of denaturation of ovalbumin (OVA) on the induction of oral sensitization in guinea pigs were examined.


METHODS
Guinea pig antibody and airway responses were assessed after 10 feedings of chemically or heat-denatured OVA or egg white (EW).


RESULTS
Their specific IgG, IgG1 and IgG2 antibody responses were orally sensitized by OVA or EW, but not by chemically or heat-denatured OVA or EW. When further exposed to 0.1% OVA or conalbumin aerosol, those fed OVA or EW, but not denatured OVA or EW, had increased pulmonary resistance and decreased tidal volume. On the other hand, in those fed denatured OVA, boiled EW or saline only, a second sensitization with 1% OVA aerosol generated antibody responses and airway hyperreactivity. Using a sandwich ELISA, guinea pig serum OVA was detected after feeding EW, but not chemically denatured or boiled EW.


CONCLUSIONS
It is likely that guinea pig gut absorption of OVA may result in oral sensitization. Chemical or heat denaturation of proteins may minimize their intestinal uptake and thus abrogates the induction of oral sensitization in guinea pigs.
Peng
H J
HJ
Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, ROC. hjpeng@vghtpe.gov.tw
Tsai
J J
JJ
Chang
Z N
ZN
Shen
H D
HD
Tsai
L C
LC
Su
S N
SN
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D016535:Bronchial Hyperreactivity / Q000276:immunology*; D004529:Egg White; D006168:Guinea Pigs; D006358:Hot Temperature; D007108:Immune Tolerance / Q000187:drug effects; D007114:Immunization; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D010047:Ovalbumin / Q000737:chemistry* / Q000276:immunology* / Q000494:pharmacology; D011489:Protein Denaturation / Q000276:immunology; D013997:Time Factors
D007074:Immunoglobulin G; D010047:Ovalbumin