Formation of phosphatidic acid and subclasses of phosphatidylethanol in human neutrophils upon interleukin-8 stimulation.
Previous studies showed that interleukin-8 (IL-8) stimulates phospholipase D hydrolysis of phosphatidylcholine to generate phosphatidic acid in human neutrophils. Phosphatidylcholine in these cells contains diacyl, alkylacyl and alkenylacyl subclasses. No studies have examined phospholipase D hydrolysis of the three subclasses of phosphatidylcholine in interleukin-8-stimulated neutrophils. We used a non-radioactive but very sensitive method to assess the relative distribution of the subclasses in phosphatidylethanol, which is derived from phospholipase D activity in ethanol-exposed neutrophils. We present evidence that the relative abundance of diacyl and alkylacyl subclasses in phosphatidylethanol is similar to that in phosphatidylcholine. Alkenylacyl subclass was also detectable in the phosphatidylethanol fraction, albeit as a minor subclass. Our findings suggest that phospholipase D catalyses the hydrolysis of diacyl, alkylacyl and alkenylacyl subclasses of phosphatidylcholine in neutrophils upon IL-8 stimulation.
Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112, USA. jtou@mailhost.tcs.tulane.edu
J S
JS
Tou
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Xu
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Wang
D002855:Chromatography, Thin Layer; D003571:Cytochalasin B / Q000494:pharmacology; D004075:Diglycerides / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology; D020404:Glycerophospholipids / Q000096:biosynthesis*; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000032:analysis / Q000096:biosynthesis*; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D015398:Signal Transduction; D013997:Time Factors
D004075:Diglycerides; D020404:Glycerophospholipids; D016209:Interleukin-8; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C051521:phosphatidylethanol; D003571:Cytochalasin B; D000431:Ethanol