Positional isomers of acetaminophen differentially induce proliferation of cultured breast cancer cells.
This study demonstrates that acetaminophen (p-acetamidophenol) stimulates proliferation of estrogen-responsive cultured breast cancer cells and assesses if the proliferative activity of p-acetamidophenol is influenced by the -OH moiety position on the benzene ring. The effects of p-, m-, and o-acetamidophenol on cell number and on percentage cells in S phase of the cell cycle were determined for two estrogen receptor positive, human breast cancer cell lines, T47D and MCF7. Therapeutic concentrations of p-acetamidophenol (0.1 mM) significantly increased breast cancer cell proliferation. The relative order of potency of isomers in stimulating proliferation in both cell types was p- > m- > o-acetamidophenol, indicating the -OH position on the benzene ring influences the proliferation output in cultured breast cancer cells.
Department of Pharmacology and Toxicology, West Virginia University, R.C. Byrd Health Sciences Center, Morgantown 26506-9142, USA.
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Harnagea-Theophilus
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Miller
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Rao
D000082:Acetaminophen / Q000737:chemistry / Q000633:toxicity*; D018712:Analgesics, Non-Narcotic / Q000737:chemistry / Q000633:toxicity*; D001943:Breast Neoplasms / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D006801:Humans; D007536:Isomerism; D009376:Neoplasms, Hormone-Dependent / Q000473:pathology*; D013056:Spectrophotometry, Ultraviolet; D014407:Tumor Cells, Cultured
D018712:Analgesics, Non-Narcotic; D000082:Acetaminophen; D004958:Estradiol