Capillary electrophoresis using diol-bonded fused-silica capillaries.
In this paper, 3-glycidoxypropyltrimethoxysilane was used to produce diol-bonded capillaries at room temperature for capillary electrophoresis (CE). A variety of standard reference compounds and authentic biological samples including ribonucleotides, peptides and proteins were used to test the columns. It was found that greatly suppressed electroosmotic flow was measured over a pH range of 3-10. Lower than 1.6% relative standard deviation (> 10 runs) in migration time was observed for the analysis of test proteins. For real samples of ribonucleotides in tumor cell extracts, approximately 1 million theoretical plates and excellent peak shapes were obtained. The high column efficiency and symmetrical peaks allowed the separation of samples with only 0.6% maximum difference in migration times. The diol-bonded fused-silica capillary columns were stable when used in a pH range of 2-8 under typical CE conditions. The column preparation method involved a simple dynamic coating procedure at room temperature, greatly simplifying the more typical static coating methods that require vacuum pumps and ovens.
Department of Microbiology, Brigham Young University, Provo, UT 84602-5700, USA.
X
X
Shao
Y
Y
Shen
K
K
O'Neill
M L
ML
Lee
D000595:Amino Acid Sequence; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D015473:Leukemia, Promyelocytic, Acute; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012265:Ribonucleotides / Q000032:analysis; D012821:Silanes / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D014407:Tumor Cells, Cultured
D007202:Indicators and Reagents; D010455:Peptides; D011506:Proteins; D012265:Ribonucleotides; D012821:Silanes; D012822:Silicon Dioxide