High-performance liquid chromatographic validated assay of doxorubicin in rat plasma and tissues.
A specific and selective high-performance liquid chromatography (HPLC) technique that requires few manipulations, and is readily adaptable to analysis for a large series of samples, has been developed for the determination of the concentration of the anticancer drug doxorubicin (DXR) in rat serum and tissues. The biological samples were efficiently deproteinised and resolved from a reversed-phase nucleosil C18 column with fluorescence detection. The validation study of the proposed method was successfully carried out in an assay range of between 5 and 5000 ng/ml and was subsequently implemented in a pharmacokinetic study of DXR in Wistar rats that were treated by intravenous administration of the drug.
Department of Pharmacy and Pharmaceutical Technology, University of Salamanca, Spain.
L
L
Alvarez-Cedrón
M L
ML
Sayalero
J M
JM
Lanao
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000032:analysis* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid; D004317:Doxorubicin / Q000032:analysis* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D012996:Solutions; D013050:Spectrometry, Fluorescence
D000903:Antibiotics, Antineoplastic; D007202:Indicators and Reagents; D012996:Solutions; D004317:Doxorubicin