Inhibition of lipid peroxidation of microsomes and mitochondria by cytosolic proteins from rat liver: effect of vitamin A.
Studies were carried out to determine the effect of rat liver cytosolic protein enriched in fatty acid binding protein on the microsomal and mitochondrial ascorbate-Fe+2 lipid peroxidation and to determine how vitamin A influences the inhibitory effect of this protein in the peroxidation process. The inhibition of light emission (maximal induced chemiluminescence) by the fatty acid binding protein containing fraction was protein concentration dependent. The inhibition of chemiluminescence produced by the addition of cytosolic protein on rat liver microsomes or mitochondria was more evident when the soluble protein obtained from the vitamin A treated group was used. The results indicated that vitamin A plays a role in protecting rat liver microsomes and mitochondria against the harmful effect of lipid peroxidation.
Palacios
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Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata CC296, Argentina.
Piergiacomi
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Catalá
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D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000494:pharmacology; D003600:Cytosol / Q000737:chemistry; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000737:chemistry*; D008163:Luminescent Measurements; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D018996:Myelin P2 Protein / Q000032:analysis / Q000494:pharmacology; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins / Q000032:analysis / Q000494:pharmacology*; D051381:Rats; D014801:Vitamin A / Q000494:pharmacology*
D002352:Carrier Proteins; C490174:Fabp7 protein, rat; D000071438:Fatty Acid-Binding Protein 7; D050556:Fatty Acid-Binding Proteins; D018996:Myelin P2 Protein; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins; D011506:Proteins; D014801:Vitamin A