Backbone dynamics of apocytochrome b5 in its native, partially folded state.
The backbone dynamics in the native state of apocytochrome b5 were studied using 15N nuclear magnetic spin relaxation measurements. The field (11.7 and 14.1 T) and temperature (10-25 degrees C) dependence of the relaxation parameters (R1, R2, and R1rho) and the 1H-15N NOE established that the protein undergoes multiple time scale internal motions related to the secondary structure. The relaxation data were analyzed with the reduced spectral density mapping approach and within the extended model-free framework. The apoprotein was confirmed to contain a disordered heme-binding loop of approximately 30 residues with dynamics on the sub-nanosecond time scale (0.6 < S2 < 0.7, 100 ps < taue < 500 ps). This loop is attached to a structured hydrophobic core, rigid on the picosecond time scale (S2 > 0.75, taue < 50 ps). The inability to fit the data for several residues with the model-free protocol revealed the presence of correlated motion. An exchange contribution was detected in the transverse relaxation rate (R2) of all residues. The differential temperature response of R2 along the backbone supported slower exchange rates for residues in the loop (tauex > 300 micros) than for the folded polypeptide chain (tauex < 150 micros). The distribution of the reduced spectral densities at the 1H and 15N frequencies followed the dynamic trend and predicted the slowing of the internal motions at 10 degrees C. Comparison of the dynamics with those of the holoprotein [Dangi, B., Sarma, S., Yan, C., Banville, D. L., and Guiles, R. D. (1998) Biochemistry 37, 8289-8302] demonstrated that binding of the heme alters the time scale of motions both in the heme-binding loop and in the structured hydrophobic core.
Department of Chemistry, The Pennsylvania State University, University Park 16802, USA.
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S
Bhattacharya
C J
CJ
Falzone
J T
JT
Lecomte
D000818:Animals; D001059:Apoproteins / Q000737:chemistry*; D003573:Cytochrome b Group / Q000737:chemistry*; D045303:Cytochromes b; D006418:Heme / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry; D011487:Protein Conformation; D017510:Protein Folding; D011522:Protons; D051381:Rats; D013696:Temperature; D013816:Thermodynamics; D013997:Time Factors
D001059:Apoproteins; D003573:Cytochrome b Group; D009587:Nitrogen Isotopes; D010446:Peptide Fragments; D011522:Protons; D006418:Heme; D045303:Cytochromes b