Comparison of flagellin genes from clinical and environmental Pseudomonas aeruginosa isolates.
Pseudomonas aeruginosa, an important opportunistic pathogen, was isolated from environmental samples and compared to clinically derived strains. While P. aeruginosa was isolated readily from an experimental mushroom-growing unit, it was found only rarely in other environmental samples. A flagellin gene PCR-restriction fragment length polymorphism analysis of the isolates revealed that environmental and clinical P. aeruginosa strains are not readily distinguishable. The variation in the central regions of the flagellin genes of seven of the isolates was investigated further. The strains used included two strains with type a genes (998 bp), four strains with type b genes (1,258 bp), and one strain, K979, with a novel flagellin gene (2,199 bp). The route by which flagellin gene variation has occurred in P. aeruginosa is discussed.
Department of Plant Pathology and Microbiology, Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, United Kingdom. alun.morgan@hri.ac.uk
J A
JA
Morgan
N F
NF
Bellingham
C
C
Winstanley
M A
MA
Ousley
C A
CA
Hart
J R
JR
Saunders
D000363:Agaricales; D001482:Base Composition; D001483:Base Sequence; D016000:Cluster Analysis; D003550:Cystic Fibrosis / Q000382:microbiology; D004269:DNA, Bacterial / Q000032:analysis; D004783:Environmental Microbiology; D005408:Flagellin / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D005408:Flagellin