Molecular genetic characterisation of intracerebrally transplanted brain tumours.
The aim of the present study was the characterisation of genetic alterations in two different experimental gliomas, induced in rats from the inbred strain BDIX by transplacental ethylnitrosourea with subsequent serial transplantation. The genes investigated have been shown previously to be altered during human glial tumour progression and include the gene for the epidermal growth factor receptor (EGFR), the genes for the cell cycle regulators cyclin dependent kinase 4 (CDK4), cyclinD1 (cycD1), the p16 gene (MTS1/INK4) and the retinoblastoma gene (RB). Using a semi-quantitative PCR-based screening method no gross alterations could be detected in these genes, demonstrating that nitrosourea-induced glial tumours of rats do not harbour those genetic changes which typically arise in human malignant gliomas. Thus, the use of this tumour model for gene therapy trials is questionable.
Schlegel
J
J
Department of Neuropathology, University Marburg, Germany.
Piontek
G
G
Kühne
C
C
Bartels
H J
HJ
Kraus
A
A
Kappler
R
R
Mennel
H D
HD
D000818:Animals; D001483:Base Sequence; D001932:Brain Neoplasms / Q000235:genetics*; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D004273:DNA, Neoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D066246:ErbB Receptors / Q000235:genetics*; D005038:Ethylnitrosourea; D016161:Genes, Retinoblastoma / Q000235:genetics; D019940:Genes, bcl-1 / Q000235:genetics*; D019942:Genes, p16 / Q000235:genetics*; D005910:Glioma / Q000235:genetics*; D008969:Molecular Sequence Data; D009368:Neoplasm Transplantation; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins; D051381:Rats; D011919:Rats, Inbred Strains
D017931:DNA Primers; D004273:DNA, Neoplasm; D011518:Proto-Oncogene Proteins; D066246:ErbB Receptors; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases; D005038:Ethylnitrosourea