Structural identification of glycerolipid molecular species isolated from cyanobacterium Synechocystis sp. PCC 6803 using fast atom bombardment tandem mass spectrometry.
Our previous works have demonstrated that fast atom bombardment tandem mass spectrometry can be a valuable tool in determining the complete structure of glycoglycerolipids and glycerophospholipids. Collision-induced dissociation of sodium-adducted molecular ions ([M + Na]+ or [M - H + 2Na]+) generates diverse product ions informative on the double-bond position in fatty acyl groups as well as the polar head group and fatty acid composition. Here we report that this direct and rapid method can be applied to the structural determination of individual molecular species of each glycerolipid class purified from the total lipid extract of cyanobacterium Synechocystis sp. PCC 6803. Especially, based on the preference for the loss of the fatty acyl group positioned at the sn-2, it was proved that all of the molecular species of diacylglycerolipids contained a palmitoyl group exclusively at the sn-2 position. Additionally, lysoglycerolipids, monoacyl forms of four major membrane diacylglycerolipids, were first isolated together from a fresh extract. Using fast atom bombardment mass spectrometry and tandem mass spectrometry, it was found that each lysoglycerolipid had a molecular species with only palmitic acid as a fatty acyl group. Thus, these compounds could be synthesized by specific enzyme-catalyzed hydrolysis of the sn-1 fatty acyl group of the corresponding diacylglycerolipids.
Kim
Y H
YH
Mass Spectrometry, Korea Basic Science Institute, Taejon, 305-600, Korea. yhkim@comp.kbsi.re.kr
Choi
J S
JS
Yoo
J S
JS
Park
Y M
YM
Kim
M S
MS
D000458:Cyanobacteria / Q000737:chemistry*; D004075:Diglycerides / Q000737:chemistry; D005989:Glycerides / Q000737:chemistry; D008055:Lipids / Q000032:analysis / Q000737:chemistry*; D008246:Lysophospholipids / Q000737:chemistry; D013058:Mass Spectrometry; D010715:Phosphatidylglycerols / Q000737:chemistry; D016339:Spectrometry, Mass, Fast Atom Bombardment
D004075:Diglycerides; D005989:Glycerides; D008055:Lipids; D008246:Lysophospholipids; D010715:Phosphatidylglycerols; C026223:lysophosphatidylglycerol