Over-expression of Candida albicans mitochondrial ribosomal protein S9 (MrpS9p) disturbs mitochondrial function in Saccharomyces cerevisiae.
A Candida albicans mitochondrial ribosomal protein S9 (MRPS9) cDNA was identified in a screen for sequences whose expression induce galactose lethality in Saccharomyces cerevisiae. MRPS9 appears to encode a protein of 346 amino acids with an N-terminal mitochondrial targeting sequence and an internal S9 signature that is conserved amongst eukaryotic mitochondrial and prokaryotic ribosomal protein S9 sequences. Expression of a GAL1-CaMRPS9 fusion in S. cerevisiae caused the slow development of a galactose-negative phenotype upon repeated subculturing, and this correlated with an increased frequency of petite mutant formation. Therefore, over-expression of CaMRPS9 interferes with S. cerevisiae mitochondrial function, which accounts for the inhibition of growth on galactose.
Department of Molecular and Cell Biology, University of Aberdeen, U.K.
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Wiltshire
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Black
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Brown
D000595:Amino Acid Sequence; D002176:Candida albicans / Q000235:genetics / Q000378:metabolism*; D004271:DNA, Fungal / Q000032:analysis; D005690:Galactose / Q000378:metabolism; D015870:Gene Expression; D008928:Mitochondria / Q000737:chemistry / Q000502:physiology*; D008969:Molecular Sequence Data; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014170:Transformation, Genetic
D004271:DNA, Fungal; C000717189:MRPS9 protein, S cerevisiae; D011993:Recombinant Fusion Proteins; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D005690:Galactose