Identification of carbohydrates on Eimeria stiedai sporozoites and their role in the invasion of cultured cells in vitro.
The carbohydrates present on Eimeria stiedai sporozoites and their functional role in the process of invasion of host cells were examined. Lectin-binding sites on the surface of sporozoites were detected by means of peroxidase-conjugated lectins. Sporozoites showed specific binding with UEA-I and PNA lectins, which bind L-fucose and D-galactose, respectively. Exposure of sporozoites to 100 microg/ml UEA-I significantly reduced their ability to invade primary rabbit liver biliary epithelial cells, but similar treatment with PNA had no such effect. Pre-incubation of these cells in Dulbecco's minimum essential medium containing 10% fetal bovine serum and 1% L-fucose suppressed the invasion activity of the sporozoites, but pre-incubation of the sporozoites in the same medium without L-fucose had no effect on cell penetration. D-galactose added to the medium had no effect on the invasion activity of sporozoites. These results indicate that L-fucose residues on E. stiedai sporozoites and L-fucose-binding sites on host cells both are associated with the recognition and/or invasion process.
John
N M
NM
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Zea
M E
ME
Kawano
T
T
Omata
Y
Y
Saito
A
A
Toyoda
Y
Y
Mikami
T
T
D000818:Animals; D001649:Bile Duct Diseases / Q000469:parasitology / Q000662:veterinary; D002241:Carbohydrates / Q000737:chemistry / Q000502:physiology*; D002478:Cells, Cultured; D003048:Coccidiosis / Q000469:parasitology / Q000662:veterinary*; D004539:Eimeria / Q000737:chemistry* / Q000502:physiology; D005243:Feces / Q000469:parasitology; D005260:Female; D005643:Fucose / Q000737:chemistry; D005690:Galactose / Q000737:chemistry; D037102:Lectins / Q000737:chemistry*; D008297:Male; D019887:Peanut Agglutinin / Q000737:chemistry; D037121:Plant Lectins; D011817:Rabbits / Q000469:parasitology*; D013047:Specific Pathogen-Free Organisms
D002241:Carbohydrates; D037102:Lectins; D019887:Peanut Agglutinin; D037121:Plant Lectins; C036850:Ulex europaeus lectins; C033619:lentil lectin; D005643:Fucose; D005690:Galactose