Genotoxicity testing of six insecticides in two crosses of the Drosophila wing spot test.
Among the great variety of genotoxicity assays available, the wing spot test in Drosophila melanogaster has some characteristics that make it very suited for the screening of genotoxic activity, i.e., it is an easy and inexpensive assay using a eukaryotic organism in vivo. One of the most interesting characteristics of the assay is its capacity to detect genotoxic activity of promutagens without the necessity of an exogenous metabolic activation system. In this paper we present results obtained with a recently developed high bioactivation cross of the wing spot test (NORR cross). The positive results obtained with the five well-known procarcinogens 7, 12-dimethylbenz[a]anthracene, N-nitrosopyrrolidine, p-dimethylaminoazobenzene, diethylnitrosamine and urethane clearly show that the NORR strains are similar to the other high bioactivation strains previously described, but they lack their methodological disadvantages. We have tested six insecticides, which are characterised by having contradictory results in other genotoxicity tests, using both the standard and the high bioactivation (NORR) cross. The six insecticides analysed are the pyrethroid allethrin, the methylenedioxyphenolic compound piperonyl butoxide, the chlorinated hydrocarbons dieldrin and endrin, and the organophosphates dimethoate and malathion. We obtained negative results for all six compounds. Our results show the suitability of the wing spot test for the evaluation of compounds at the first level of genotoxicity testing.
Department of Animal Biology and Genetics, Faculty of Sciences, University of Basque Country, E-48080, Bilbao, Spain.
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Osaba
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Aguirre
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Alonso
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Graf
D000818:Animals; D002273:Carcinogens / Q000032:analysis; D003433:Crosses, Genetic; D004026:Dieldrin / Q000633:toxicity; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics*; D007306:Insecticides / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000379:methods*; D009153:Mutagens / Q000032:analysis*; D014921:Wings, Animal / Q000187:drug effects; D004124:p-Dimethylaminoazobenzene / Q000633:toxicity
D002273:Carcinogens; D007306:Insecticides; D009153:Mutagens; D004124:p-Dimethylaminoazobenzene; D004026:Dieldrin