Common fragile site expression and genetic predisposition to breast cancer.
The expression of common fragile sites induced by aphidicolin and caffeine was evaluated on prometaphase obtained from the peripheral blood lymphocytes of 35 women with breast cancer, their 35 clinically healthy female family members, and 20 sex- and age-matched normal controls. As a result of the cytogenetic and statistical evaluation, the number of damaged cells, chromosomal aberrations, and expression frequencies of fragile sites detected in patients with breast cancer and their first-degree relatives were found to be significantly higher than those in the control group. Our findings indicate an increased genetic instability in women with breast carcinomas and their relatives. Therefore, fragile sites may be used as a reliable marker for defining genetic susceptibility to cancer in general.
Department of Molecular Biology, Uludağ University College of Science, Bursa, Turkey.
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Ceçener
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Egeli
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Taşdelen
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Tunca
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Duman
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Kizil
D016590:Aphidicolin / Q000633:toxicity; D001943:Breast Neoplasms / Q000235:genetics* / Q000517:prevention & control; D002110:Caffeine / Q000633:toxicity; D002869:Chromosome Aberrations; D043283:Chromosome Fragile Sites; D002873:Chromosome Fragility / Q000235:genetics*; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D010375:Pedigree; D012307:Risk Factors
D005819:Genetic Markers; D016590:Aphidicolin; D002110:Caffeine