Galectin 1 modulates attachment, spreading and migration of cultured vascular smooth muscle cells via interactions with cellular receptors and components of extracellular matrix.
Galectin 1 (Gal-1), a lactose-binding lectin, is a component of vascular extracellular matrix and secreted by human vascular smooth muscle cells (SMCs). The purpose of this study was to investigate a possible role of Gal-1 in controlling adhesion and migration of cultured human vascular SMCs. Gal-1 co-localised with laminin and cellular fibronectin in extracellular matrix (ECM) secreted by cultured human vascular SMCs. Recombinant glutathione S-transferase (GST)-Gal-1 fusion protein bound to laminin and cellular fibronectin in ELISA. GST-Gal-1 inhibited SMC attachment to laminin via interactions with both SMCs and laminin. GST-Gal-1 inhibited SMC spreading on plastic or on laminin, but not on cellular fibronectin. GST-Gal-1 modulated SMC migration on laminin and inhibited migration on cellular fibronectin. GST-Gal-1 bound to several 35S-labelled proteins in SMC extracts including laminin and alpha1beta1 integrin, identified by depletion of SMC protein extracts with respective antibodies. We conclude that Gal-1 is able to modulate SMC attachment, spreading and migration via interactions with ECM proteins and alpha1beta1 integrin.
Department of Medicine, Division of Cardiology, University of Leicester, Glenfield General Hospital, Leicester, UK. em9@leicester.ac.uk
E P
EP
Moiseeva
E L
EL
Spring
J H
JH
Baron
D P
DP
de Bono
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D037483:Galectin 1; D005982:Glutathione Transferase / Q000235:genetics; D006388:Hemagglutinins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D039222:Integrin alpha1beta1; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011993:Recombinant Fusion Proteins
D000276:Adjuvants, Immunologic; D005353:Fibronectins; D037483:Galectin 1; D006388:Hemagglutinins; D039222:Integrin alpha1beta1; D016023:Integrins; D007797:Laminin; D010982:Platelet-Derived Growth Factor; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase