The role of the sympathetic nervous system in the regulation of leptin synthesis in C57BL/6 mice.
The objectives of this study were to determine whether leptin synthesis is regulated by the sympathetic nervous system and if so whether beta-adrenergic receptors mediate this effect. We show that sympathetic blockade by reserpine increases leptin mRNA levels in brown but not white adipose tissue, while acute cold-exposure decreases leptin expression 10-fold in brown adipose tissue and 2-fold in white adipose tissue. The cold-induced reduction in leptin mRNA can be prevented by a combination of propranolol and SR 59230A but not by either antagonist alone, indicating that beta3-adrenergic receptors and classical beta1/beta2-adrenergic receptors both mediate responses to sympathetic stimulation. Circulating leptin levels reflect synthesis in white adipose tissue but not in brown adipose tissue.
Evans
B A
BA
Department of Pharmacology, Monash University, Clayton, Victoria, Australia. bronwyn.evans@med.monash.edu.au
Agar
L
L
Summers
R J
RJ
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003080:Cold Temperature; D020738:Leptin; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011412:Propanolamines / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3; D054411:Receptors, Leptin; D012110:Reserpine / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000378:metabolism*
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D020738:Leptin; D011412:Propanolamines; D011506:Proteins; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D012110:Reserpine; D011433:Propranolol