Purification and primary structure of low molecular mass peptides from scorpion (Buthus sindicus) venom.
The primary structures of four low molecular mass peptides (Bs 6, 8, 10 and 14) from scorpion Buthus sindicus were elucidated via combination of Edman degradation and matrix-assisted laser desorption ionization mass spectrometry. Bs 8 and 14 are cysteine-rich, thermostable peptides composed of 35-36 residues with molecular weights of 3.7 and 3.4 kDa, respectively. These peptides show close sequence homologies (55-78%) with other scorpion chlorotoxin-like short-chain neurotoxins (SCNs) containing four intramolecular disulfide bridges. Despite the sequence variation between these two peptides (37% heterogeneity) their general structural organization is very similar as shown by their clearly related circular dichroism spectra. Furthermore, Bs6 is a minor component, composed of 38 residues (4.1 kDa) containing six half-cystine residues and having close sequence identities (40-80%) with charybdotoxin-like SCNs containing three disulfide bridges. The non-cysteinic, bacic and thermolabile Bs10 is composed of 34 amino acid residues (3.7 kDa), and belongs to a new class of peptides, with no sequence resemblance to any other so far reported sequence isolated from scorpions. Surprisingly, Bs10 shows some limited sequence analogy with oocyte zinc finger proteins. Results of these studies are discussed with respect to their structural similarities within the scorpion LCNs, SCNs and other biologically active peptides.
International Centre for Chemical Sciences, University of Karachi, Pakistan. saali66@hotmail.com
S A
SA
Ali
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S
Stoeva
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J
Schütz
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Kayed
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Abassi
Z H
ZH
Zaidi
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Voelter
D000818:Animals; D018999:Charybdotoxin / Q000737:chemistry / Q000235:genetics; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification*; D012604:Scorpion Venoms / Q000737:chemistry* / Q000235:genetics; D012605:Scorpions; D017386:Sequence Homology, Amino Acid
D010446:Peptide Fragments; D012604:Scorpion Venoms; C091539:Chlorotoxin; D018999:Charybdotoxin; D003545:Cysteine