Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display.
Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Emu-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retroelement that was significantly overexpressed in a subset of lymphomas as compared with non-lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.
Department of Molecular Cell Biology, Leiden University, The Netherlands.
C W
CW
van der Houven van Oordt
T G
TG
Schouten
A J
AJ
van der Eb
M L
ML
Breuer
D000818:Animals; D003001:Cloning, Molecular; D003626:Data Display; D004076:Digoxigenin; D015972:Gene Expression Regulation, Neoplastic; D016259:Genes, myc; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics; D008049:Lipase / Q000235:genetics; D008223:Lymphoma / Q000235:genetics*; D051379:Mice; D009381:Neoplasms, Radiation-Induced / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D018626:Retroelements; D013602:T-Lymphocytes, Cytotoxic / Q000201:enzymology; D014158:Transcription, Genetic; D014965:X-Rays
D018626:Retroelements; D005982:Glutathione Transferase; D008049:Lipase; D004076:Digoxigenin