Histopathological evidence that spermatogonia are the target cells of 2-bromopropane.
To confirm the target cell of 2-bromopropane within the testis, 1355 mg/kg of 2-bromopropane was subcutaneously injected to rats for 1-5 days and the numbers of spermatogonia and spermatocytes were examined 6 h after each last injection. The number of stage I spermatogonia decreased after the first 2-bromopropane injection and the number of spermatogonia at the other stages also decreased following repetitive injection. The number of these spermatogonia decreased further by the repetition of 2-bromopropane injection. In addition, the delay in mitotic division of type B spermatogonia was frequently observed after the fifth 2-bromopropane injection. The number of stage I pachytene spermatocytes also decreased slightly after the first 2-bromopropane injection, although it did not decrease further following repetitive injection. Therefore, we concluded that spermatogonia are the target cells of 2-bromopropane in rats.
Department of Hygiene, Faculty of Medicine, Kyushu University, Fukuoka, Japan. momura@eisei.med.kyushu-u.ac.jp
M
M
Omura
Y
Y
Romero
M
M
Zhao
N
N
Inoue
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D006842:Hydrocarbons, Brominated / Q000633:toxicity*; D008297:Male; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012671:Seminiferous Tubules / Q000166:cytology / Q000187:drug effects; D013090:Spermatocytes / Q000187:drug effects; D013093:Spermatogonia / Q000187:drug effects*; D013737:Testis / Q000166:cytology* / Q000187:drug effects
D006842:Hydrocarbons, Brominated; D009153:Mutagens; C103736:2-bromopropane