Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human.
Cytoplasmic dynein is a large multisubunit microtubule-based motor protein, which mediates movement of numerous intracellular organelles. We report here the identification of the human homologue of cytoplasmic dynein intermediate chain 1 gene (DNCI1) located on human chromosome 7q21.3-q22.1. The mouse orthologue (Dnci1) was identified along with another highly related gene, Dnci2, and their RNA in situ expression patterns were examined during mouse embryogenesis. Dnci1 was found to have a highly restricted expression domain in the developing forebrain as well as the peripheral nervous system (PNS), while Dnci2 displayed a broad expression profile throughout the entire central nervous system and most of the PNS. A dynamic expression profile was also found for Dnci2 in the developing mouse limb bud. The data presented here provide a framework for the further analysis of the functional role of Dnci1 and Dnci2 in mouse and DNCI1 in human.
Department of Molecular and Medical Genetics, University of Toronto, The Hospital for Sick Children, Ontario, Canada.
M A
MA
Crackower
D S
DS
Sinasac
J
J
Xia
J
J
Motoyama
M
M
Prochazka
J M
JM
Rommens
S W
SW
Scherer
L C
LC
Tsui
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast; D004398:Dyneins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D006801:Humans; D017403:In Situ Hybridization; D018878:Limb Buds / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution
D004398:Dyneins