Effects of vasoactive mediators on the rat lung pericyte: quantitative analysis of contraction on collagen lattice matrices.
Pericytes are contractile cells of the microvasculature which may contribute to the hypotension and increase in permeability that are present during inflammation and late-stage sepsis. The purpose of this study was to examine the contractile effects, if any, of septic modulators on the lung pericyte. Contractile effects were qualitatively examined using a previously developed silicone rubber method. This study further demonstrates a quantitative method for measuring the contraction of lung pericytes cultured on a collagen lattice. Contraction was measured by the change in collagen matrix area in response to vasoactive stimuli. Bradykinin and serotonin significantly increased contraction in a dose-dependent manner, with a maximum increase in contraction twice that of control. Forskolin and adenosine caused relaxation, also in a significant dose-dependent manner, with a maximum decrease in contraction of 80 and 30-40%, respectively. Histamine had no effect on contractility in either the silicone rubber or the collagen lattice assay. These results show that the lung pericyte, like the retinal pericyte, is a contractile cell and can be stimulated to contract or relax in vitro by the presence of certain inotropic agents present during inflammation and sepsis. These responses may play a role in microvascular regulation.
Department of Surgery, Wayne State University, Detroit, Michigan, 48201, USA.
C L
CL
Speyer
C P
CP
Steffes
J L
JL
Ram
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology; D005576:Colforsin / Q000494:pharmacology; D003094:Collagen; D066298:In Vitro Techniques; D008168:Lung / Q000098:blood supply* / Q000166:cytology; D008297:Male; D008833:Microcirculation / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D020286:Pericytes / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012701:Serotonin / Q000494:pharmacology; D012826:Silicone Elastomers; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology*; D014665:Vasodilator Agents / Q000494:pharmacology
D012826:Silicone Elastomers; D014662:Vasoconstrictor Agents; D014665:Vasodilator Agents; D005576:Colforsin; D012701:Serotonin; D003094:Collagen; D000241:Adenosine; D001920:Bradykinin