Multiplex sequence analysis demonstrates the competitive growth advantage of the A-to-G mutants of clarithromycin-resistant Helicobacter pylori.
Clarithromycin resistance in Helicobacter pylori is due to point mutation within the 23S rRNA. We examined the growth rates of different types of site-directed mutants and demonstrated quantitatively the competitive growth advantage of A-to-G mutants over other types of mutants by a multiplex sequencing assay. The results provide a rational explanation of why A-to-G mutants are predominantly observed among clarithromycin-resistant clinical isolates.
Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada.
G
G
Wang
M S
MS
Rahman
M Z
MZ
Humayun
D E
DE
Taylor
D000409:Alanine / Q000235:genetics*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001345:Autoradiography; D001483:Base Sequence; D017291:Clarithromycin / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D004352:Drug Resistance, Microbial; D005998:Glycine / Q000235:genetics*; D016480:Helicobacter pylori / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D017354:Point Mutation
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D017291:Clarithromycin; D000409:Alanine; D005998:Glycine