Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells.
The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1 Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc2 (26.5%), GlcNAc1Man3Fuc1Xyl1GlcNAc2 (21.7%), Man3 Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that alpha(1-->3) fucosylation of N-glycans does occur but beta(1-->4) galactosylation of the sugar chains does not in the tobacco cultured cells.
International Center for Biotechnology, Osaka University, Japan.
N Q
NQ
Palacpac
Y
Y
Kimura
K
K
Fujiyama
T
T
Yoshida
T
T
Seki
D002240:Carbohydrate Sequence; D002460:Cell Line; D006023:Glycoproteins / Q000737:chemistry*; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry*; D010947:Plants, Toxic; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification; D014026:Nicotiana / Q000737:chemistry*
D006023:Glycoproteins; D009844:Oligosaccharides; D010940:Plant Proteins; D011134:Polysaccharides