Structure analysis of a fusogenic peptide sequence from the sea urchin fertilization protein bindin.
The structure of "B18", an 18-residue fusogenic peptide from the sea urchin fertilization protein bindin, was investigated in several membrane-mimicking environments with circular dichroism and nuclear magnetic resonance spectroscopy. The fully conserved peptide sequence represents the minimal functional part of the 24 kDa protein, which can bind to membranes and induce fusion of lipid vesicles. The B18 peptide undergoes a coil-helix transition in the presence of TFE, showing a transient tendency to self-associate. Its NMR structure in 30% TFE exhibits two helical regions at either side, connected by a flexible loop. In DPC and SDS detergent micelles, this loop becomes distinctly bent, presumably due to the high degree of curvature of the micelles. The loop contains a histidine-rich motif for binding zinc, which is required for the fusogenic function of the peptide. Therefore, we monitored the structural response of B18 and of recombinant bindin toward this ion. Like TFE, and in a mutually cooperative manner, zinc induces a partially helical structure in both the peptide and the protein. Complex formation via the histidine residues rigidifies the flexible loop and is accompanied by self-association of the molecules. The data suggest that the zinc-bound functional state is a continuous amphipathic alpha-helix, bearing some resemblance to a leucine zipper. Two hydrophobic patches on one face could favorably penetrate into a membrane, while two arginines on the other face could interact with lipid phosphate groups. The three-dimensional model of the B18 sequence thus contributes to a better understanding of peptide-induced vesicle fusion in general, and of the lipid-protein interactions of sperm bindin in particular.
Institut für Molekularbiologie, Friedrich-Schiller-Universität Jena, Germany.
R W
RW
Glaser
M
M
Grüne
C
C
Wandelt
A S
AS
Ulrich
D000595:Amino Acid Sequence; D000818:Animals; D002021:Buffers; D011232:Chemical Precipitation; D002942:Circular Dichroism; D003902:Detergents; D005306:Fertilization; D006023:Glycoproteins / Q000737:chemistry* / Q000502:physiology; D008561:Membrane Fusion; D008823:Micelles; D008958:Models, Molecular; D008969:Molecular Sequence Data; D019906:Nuclear Magnetic Resonance, Biomolecular; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000502:physiology; D017433:Protein Structure, Secondary; D011956:Receptors, Cell Surface; D012617:Sea Urchins; D012995:Solubility; D013329:Structure-Activity Relationship; D014270:Trifluoroethanol / Q000737:chemistry; D015032:Zinc / Q000737:chemistry
D002021:Buffers; D003902:Detergents; D006023:Glycoproteins; D008823:Micelles; D010455:Peptides; D011956:Receptors, Cell Surface; C035027:egg surface sperm receptor; D014270:Trifluoroethanol; D015032:Zinc