Involvement of two plasmids in the degradation of carbaryl by Arthrobacter sp. strain RC100.
A bacterium capable of utilizing carbaryl (1-naphthyl N-methylcarbamate) as the sole carbon source was isolated from carbaryl-treated soil. This bacterium was characterized taxonomically as Arthrobacter and was designated strain RC100. RC100 hydrolyzes the N-methylcarbamate linkage to 1-naphthol, which was further metabolized via salicylate and gentisate. Strain RC100 harbored three plasmids (designated pRC1, pRC2, and pRC3). Mutants unable to degrade carbaryl arose at a high frequency after treating the culture with mitomycin C. All carbaryl-hydrolysis-deficient mutants (Cah-) lacked pRC1, and all 1-naphthol-utilization-deficient mutants (Nat-) lacked pRC2. The plasmid-free strain RC107 grew on gentisate as a carbon source. These two plasmids could be transferred to Cah- mutants or Nat- mutants by conjugation, resulting in the restoration of the Cah and Nah phenotypes.
Hayatsu
M
M
Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan. ahmhaya@agr.shizuoka.ac.jp
Hirano
M
M
Nagata
T
T
D001173:Arthrobacter / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002219:Carbamates / Q000378:metabolism; D012721:Carbaryl / Q000378:metabolism*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D004275:DNA, Ribosomal / Q000032:analysis / Q000235:genetics; D007306:Insecticides / Q000378:metabolism*; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D010957:Plasmids / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology
D002219:Carbamates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007306:Insecticides; D012336:RNA, Ribosomal, 16S; C020401:N-methylcarbamate; D012721:Carbaryl