Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity.
Calreticulin (CRT), a peptide-binding heat shock protein (HSP) of the endoplasmic reticulum (ER), has been shown previously to associate with peptides transported into the ER by transporter associated with antigen processing (Spee, P., and J. Neefjes. 1997. Eur. J. Immunol. 27: 2441-2449). Our studies show that CRT preparations purified from tumors elicit specific immunity to the tumor used as the source of CRT but not to an antigenically distinct tumor. The immunogenicity is attributed to the peptides associated with the CRT molecule and not to the CRT molecule per se. It is further shown that CRT molecules can be complexed in vitro to unglycosylated peptides and used to elicit peptide-specific CD8(+) T cell response in spite of exogenous administration. These characteristics of CRT closely resemble those of HSPs gp96, hsp90, and hsp70, although CRT has no apparent structural homologies to them.
Center for Immunotherapy of Cancer and Infectious Diseases, University of Connecticut School of Medicine, Farmington, Connecticut 06030, USA.
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D000818:Animals; D017951:Antigen Presentation; D000951:Antigens, Neoplasm / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000276:immunology* / Q000378:metabolism; D037282:Calreticulin; D004721:Endoplasmic Reticulum / Q000276:immunology*; D005354:Fibrosarcoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018832:Molecular Chaperones / Q000276:immunology* / Q000378:metabolism; D010455:Peptides / Q000276:immunology* / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D012261:Ribonucleoproteins / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
D000951:Antigens, Neoplasm; D002135:Calcium-Binding Proteins; D037282:Calreticulin; D018832:Molecular Chaperones; D010455:Peptides; D012261:Ribonucleoproteins; C052646:sarcoma glycoprotein gp96 rejection antigens