Buthionine sulfoximine prevents the reduction of the genotoxic activity of maleic hydrazide by soil humic substances in Vicia faba seedlings.
A significant reduction of the genotoxic effects caused by herbicide maleic hydrazide (MH) in Vicia faba seedlings was observed to be induced by a growth step in an organic soil as well as by a pretreatment with highly purified humic substances. In addition, such protective activity was resulted quite similar to that observed when the conditioning pretreatment was carried out with metal salts, so suggesting the involvement of the GSH biosynthesis in determining the protective activity observed. In agreement with this hypothesis, a previous exposure to buthionine sulfoximine (BSO), an inhibitor of the phytochelatins production, through the inhibition of GSH synthesis, prevented the reduction of the genotoxic activity of MH. The findings provide evidence for the involvement of the GSH biosynthesis pathway in determining the antigenotoxic activity revealed and suggest a possible involvement of the phytochelatins in this process. However, yet to be clarified is whether the stimulation of GSH production results as a consequence of a nonspecific influence on the protein synthesis by humic substances or of its direct activation due to the presence, as contaminants, of some heavy metals in both organic soil and humic acids extracts.
Centro di Genetica Evoluzionistica, C.N.R., Università La Sapienza, Rome, Italy.demarcoa@tin.it
A
A
De Marco
C
C
De Simone
C
C
D'Ambrosio
M
M
Owczarek
D016587:Antimutagenic Agents / Q000494:pharmacology*; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D005978:Glutathione / Q000502:physiology*; D006540:Herbicides / Q000633:toxicity*; D006812:Humic Substances / Q000494:pharmacology*; D008300:Maleic Hydrazide / Q000378:metabolism / Q000633:toxicity*; D008670:Metals / Q000494:pharmacology; D010944:Plants / Q000187:drug effects; D012987:Soil
D016587:Antimutagenic Agents; D006540:Herbicides; D006812:Humic Substances; D008670:Metals; D012987:Soil; D019328:Buthionine Sulfoximine; D005978:Glutathione; D008300:Maleic Hydrazide