Simple and rapid extraction method of total egg lipids for determining organochlorine pesticides in the egg.
A simple and rapid extraction method of total egg lipids for determination of organochlorine pesticides (OCPs) in the egg was developed. After being mixed with anhydrous sodium sulphate, the extraction of lipids in egg yolk was performed using n-hexane-acetonitrile (2:1, v/v). Troublesome emulsions did not occur. Using the present method, an average of 3.03 g of egg lipids was collected from 10 g of egg yolk. Compared with classical methods, the present method is handy; needs much shorter analysis time and less requirement of solvents and has higher efficiency of egg lipid extraction and higher recoveries of OCPs.
Faculty of Human Life Science, Osaka City University, Japan. furusawa@life.osaka-cu.ac.jp
N
N
Furusawa
A
A
Ozaki
M
M
Nakamura
Y
Y
Morita
K
K
Okazaki
D002849:Chromatography, Gas; D002850:Chromatography, Gel; D004530:Egg Yolk / Q000737:chemistry*; D004531:Eggs; D005506:Food Contamination; D006843:Hydrocarbons, Chlorinated; D007306:Insecticides / Q000032:analysis*; D008055:Lipids / Q000032:analysis* / Q000302:isolation & purification; D013431:Sulfates
D006843:Hydrocarbons, Chlorinated; D007306:Insecticides; D008055:Lipids; D013431:Sulfates; C012036:sodium sulfate