Genetic dissection of the molecular target(s) of anesthetics with the gene knockout approach in mice.
Techniques have recently been developed that enable the creation of mice that harbor specific, predetermined genetic changes. These 'gene knockout mice', which contain a single genetic modification that is determined by the investigator, can subsequently be analyzed with tests that span the molecular, cellular, and behavioral levels. Application of such a multi-level approach to mechanisms of drug action should ultimately allow general anesthetic responses to be properly attributed to a molecular site.
Department of Anesthesiology and Critical Care Medicine, University of Pittsburgh, PA 15261, USA. homanics@smtp.anes.upmc.edu
G E
GE
Homanics
J J
JJ
Quinlan
R
R
Mihalek
L L
LL
Firestone
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D018390:Gene Targeting; D006801:Humans; D051379:Mice; D018345:Mice, Knockout / Q000235:genetics*; D011955:Receptors, Drug / Q000187:drug effects / Q000235:genetics*
D000777:Anesthetics; D011955:Receptors, Drug