The amino acid sequence of wood duck lysozyme.
The amino acid sequence of wood duck (Aix sponsa) lysozyme was analyzed. Carboxymethylated lysozyme was digested with trypsin and the resulting peptides were sequenced. The established amino acid sequence had the highest similarity to duck III lysozyme with four amino acid substitutions, and had eighteen amino acid substitutions from chicken lysozyme. The valine at position 75 was newly detected in chicken-type lysozymes. In the active site, Tyr34 and Glu57 were found at subsites F and D, respectively, when compared with chicken lysozyme.
Araki
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Department of Biochemistry, School of Agriculture, Kyushu Tokai University, Kumamoto, Japan. taraki@as-1.ktokai-u.ac.jp
Torikata
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D000595:Amino Acid Sequence; D000818:Animals; D020134:Catalytic Domain / Q000235:genetics; D002645:Chickens; D002851:Chromatography, High Pressure Liquid; D004372:Ducks / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000302:isolation & purification; D010446:Peptide Fragments / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D010446:Peptide Fragments; D009113:Muramidase