Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant.
We have discovered a multienzymatic complex in fresh young sugarcane leaves. This complex is constituted of three enzymes: PEPcase, NADP-MDH and malic enzyme. After successive molecular sieving chromatography, we have obtained a highly purified sample of the complex which has a molecular weight of 711 kDa. Its functional interest has been evaluated by comparing the kinetic properties of the enzymes in their free forms to those in their complexed form. We show that the association of the three enzymes leads to important changes in their respective kinetic properties.
Laboratoire de biochimie et génétique moléculaire, faculté des sciences et technologies, université de la Réunion, Saint-Denis Messag, France.
P
P
Baret
M
M
Cesari
C
C
Queiroz
C
C
Rouch
J C
JC
Meunier
F
F
Cadet
D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D017886:Durapatite; D007700:Kinetics; D008291:Malate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D050538:Malate Dehydrogenase (NADP+); D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves; D010945:Plants, Edible / Q000201:enzymology*
D009097:Multienzyme Complexes; D017886:Durapatite; D008291:Malate Dehydrogenase; D050538:Malate Dehydrogenase (NADP+); D010730:Phosphoenolpyruvate Carboxylase