Clonogenic inactivation of colon cancer-derived cells treated with 5-fluorouracil and indomethacin in hybrid spheroids.
The clonogenic hybrid spheroid assay has been used to determine the toxicity of 5-fluorouracil (5-FU), alone or in combination with indomethacin, in LoVo cells (a human colon adenocarcinoma line). The principal finding was that 5-FU toxicity, determined as loss of colony-forming ability, increased as a function of dose (concentration x duration of exposure), and that indomethacin causes a generalized alleviation of 5-FU toxicity, but only if given concurrently with 5-FU. The implications of these findings in the control of cancer cells by 5-FU are discussed.
Djordjevic
B
B
Department of Radiation Oncology, State University of New York, Health Science Center at Brooklyn 11203, USA.
Lange
C S
CS
Schwartz
M S
MS
Rotman
M
M
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology*; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology* / Q000627:therapeutic use; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000473:pathology*; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D005472:Fluorouracil / Q000494:pharmacology* / Q000627:therapeutic use; D006367:HeLa Cells; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology* / Q000627:therapeutic use; D018874:Spheroids, Cellular / Q000187:drug effects*; D014407:Tumor Cells, Cultured
D000964:Antimetabolites, Antineoplastic; D016861:Cyclooxygenase Inhibitors; D005472:Fluorouracil; D007213:Indomethacin