Platelet-bound immunogloblins before and after platelet transfusion: measurement of in vivo binding.
In routine tests to investigate immunological mechanisms as a cause for enhanced destruction of transfused platelets; serum from the patient is tested against a panel of donor lymphocytes and platelets to demonstrate the presence of antibodies against HLA or platelet specific antibodies. Here we describe a flow cytometric technique in which in vivo binding of immunoglobulins (Ig) is measured. By comparing the histograms of the platelet suspensions before and after transfusion, four different patterns were obtained: no Ig binding before and after transfusion (pattern 1), pre-existent Ig binding (patterns 2 and 3) or preferential Ig binding to the transfused platelets (pattern 4). This technique was tested in 164 random platelet transfusions and 34 HLA-matched platelet transfusions. A statistically significant association was found between pattern 1 and a negative result of the indirect platelet immunofluorescence test for the detection of antibodies in the serum taken before the transfusion studied or a good platelet recovery and between pattern 4 and a positive indirect platelet immunofluorescence test or a low platelet recovery.
Department of Internal Medicine, Zuiderziekenhuis, Rotterdam, The Netherlands.
M D
MD
Levin
W
W
de Vries
J
J
de Veld
D
D
Doekharan
B
B
van der Holt
M B
MB
van 't Veer
D015551:Autoimmunity; D001792:Blood Platelets / Q000276:immunology*; D005434:Flow Cytometry; D019337:Hematologic Neoplasms / Q000276:immunology* / Q000628:therapy; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D017713:Platelet Transfusion / Q000379:methods*; D011897:Random Allocation
D007074:Immunoglobulin G