Synthesis and utilization of 13C(8)-enriched purines.
A new and more efficient method is presented for the synthesis of 13C(8)-enriched adenine. In addition, we present for the first time the synthesis of 13C(8)-enriched 2-aminopurine and purine. All three analogues have been converted to the corresponding ribonucleosides. The adenine analogue has been further converted to the 2'-deoxy-nucleoside and incorporated into a synthetic oligonucleotide. Data is presented demonstrating the utility of 13C-enrichment in heteronuclear isotope-edited NMR spectra.
LaFrancois
C J
CJ
Division of Pediatrics, City of Hope National Medical Center, Duarte, CA 91010, USA.
Fujimoto
J
J
Sowers
L C
LC
D001483:Base Sequence; D002247:Carbon Isotopes; D007202:Indicators and Reagents; D007553:Isotope Labeling / Q000379:methods; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011687:Purines / Q000138:chemical synthesis* / Q000737:chemistry
D002247:Carbon Isotopes; D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D011687:Purines