2D protein electrophoresis: can it be perfected?
23 years after O'Farrel developed two-dimensional gel electrophoresis we still debate if the technique can be improved, or if there are other alternative separation technologies that can challenge its central position in proteomic projects. These questions are relevant as the pharmaceutical industry expects proteomic studies to provide novel protein targets for drug discovery and diagnostics. In our opinion, there are various aspects of the technology that can be improved, including resolution, sample preparation and detection, but so far there is no alternative technique(s) available, or any under development, that can replace it.
Department of Medical Biochemistry Danish Centre for Human Genome Research University of Aarhus Ole Worms Allé Building 170 DK-8000 Aarhus C Denmark. jec@biokemi.au.dk
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D000818:Animals; D001671:Biochemistry / Q000379:methods; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005057:Eukaryotic Cells / Q000737:chemistry; D006801:Humans; D011506:Proteins / Q000032:analysis / Q000737:chemistry
D011506:Proteins