Separation of promethazine and thioridazine using capillary electrophoresis with end-column amperometric detection.
Promethazine and thioridazine were separated and detected by capillary electrophoresis with end-column amperometric detection. The influence of pH value on oxidation potential, the peak current and the resolution were studied and the following conditions was selected: 0.03 M Na2HPO4 and 0.015 M citric acid at pH 3.0, detection potential at 1.10 V. The detection limits of these two substances were in the range of 10(-8) mol/l. The linear range spanned two to three orders of magnitude. This method was applied to the detection of promethazine and thioridazine spiked in urine.
Wang
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Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, PR China.
Lu
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X
Wu
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Wang
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E
D014150:Antipsychotic Agents / Q000302:isolation & purification* / Q000652:urine; D004563:Electrochemistry; D004566:Electrodes; D019075:Electrophoresis, Capillary; D006634:Histamine H1 Antagonists / Q000302:isolation & purification* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D011398:Promethazine / Q000302:isolation & purification* / Q000652:urine; D015203:Reproducibility of Results; D013881:Thioridazine / Q000302:isolation & purification* / Q000652:urine
D014150:Antipsychotic Agents; D006634:Histamine H1 Antagonists; D011398:Promethazine; D013881:Thioridazine