Peritoneal endometriotic lesions differentially express a haptoglobin-like gene.
A unique glycoprotein, called endometriosis protein-I (ENDO-I), has previously been shown to be synthesized and secreted by rat and human endometriotic explants. Furthermore, the N-terminal amino acid sequence analysis showed that ENDO-I shares homology with haptoglobin. The present study was designed to determine this sequence of ENDO-I cDNA from human peritoneal endometriotic lesions and to determine the relative expression of the ENDO-I gene in several human tissues. Poly A-enriched RNA was isolated and reverse-transcribed. To determine the sequence of ENDO-I cDNA, a polymerase chain reaction was performed on cDNA from human endometriotic lesions and a gene-specific primer based on the human haptoglobin sequence. A similar procedure was followed to assess the relative expression of the ENDO-I gene in various tissues. Glyceraldehyde-3-phosphate-dehydrogenase was used as an internal control. ENDO-I gene expression was quantified by densitometry. Sequence analysis of ENDO-I cDNA identified 873 nucleotides that displayed 99.4% homology with the human haptoglobin beta-chain. Relative expression of ENDO-I mRNA by peritoneal endometriotic lesions was 19-fold greater than peritoneum, 28-fold greater than endometrioma and 37-fold greater than eutopic endometrium (P<0.01). Haptoglobin-like ENDO-I may be associated with localized angiogenesis and altered immune response involved with the aetiology/pathophysiology of endometriosis.
Piva
M
M
Department of Obstetrics and Gynecology, The University of Missouri, Columbia 65212, USA.
Sharpe-Timms
K L
KL
D000293:Adolescent; D000328:Adult; D000818:Animals; D001483:Base Sequence; D016022:Case-Control Studies; D004715:Endometriosis / Q000235:genetics*; D004717:Endometrium / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation; D006023:Glycoproteins / Q000235:genetics*; D006242:Haptoglobins / Q000235:genetics*; D006801:Humans; D008099:Liver / Q000502:physiology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010532:Peritoneal Diseases / Q000235:genetics*; D051381:Rats; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D006023:Glycoproteins; D006242:Haptoglobins; C112213:endometriosis protein-1