Complete exon-intron organization of the mouse fibulin-1 gene and its comparison with the human fibulin-1 gene.
Fibulin-1 is a 90 kDa calcium-binding protein present in the extracellular matrix and in the blood. Two major variants, C and D, differ in their C-termini as well as the ability to bind the basement membrane protein nidogen. Here we characterized genomic clones encoding the mouse fibulin-1 gene, which contains 18 exons spanning at least 75 kb of DNA. The two variants are generated by alternative splicing of exons in the 3' end. By searching the database we identified most of the exons encoding the human fibulin-1 gene and showed that its exon-intron organization is similar to that of the mouse gene.
Department of Dermatology and Cutaneous Biology, Jefferson Institute of Molecular Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA.
T C
TC
Pan
G
G
Kostka
R Z
RZ
Zhang
R
R
Timpl
M L
ML
Chu
D017398:Alternative Splicing / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics; D003001:Cloning, Molecular; D003360:Cosmids / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D016208:Databases, Factual; D005091:Exons / Q000235:genetics; D015698:Genomic Library; D006801:Humans; D007438:Introns / Q000235:genetics; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics; D012689:Sequence Homology, Nucleic Acid
D002135:Calcium-Binding Proteins; D018076:DNA, Complementary; D020033:Protein Isoforms; C060820:fibulin