Actions of a pair of identified cerebral-buccal interneurons (CBI-8/9) in Aplysia that contain the peptide myomodulin.
A combination of biocytin back-fills of the cerebral-buccal connectives and immunocytochemistry of the cerebral ganglion demonstrated that of the 13 bilateral pairs of cerebral-buccal interneurons in the cerebral ganglion, a subpopulation of 3 are immunopositive for the peptide myomodulin. The present paper describes the properties of two of these cells, which we have termed CBI-8 and CBI-9. CBI-8 and CBI-9 were found to be dye coupled and electrically coupled. The cells have virtually identical properties, and consequently we consider them to be "twin" pairs and refer to them as CBI-8/9. CBI-8/9 were identified by electrophysiological criteria and then labeled with dye. Labeled cells were found to be immunopositive for myomodulin, and, using high pressure liquid chromatography, the cells were shown to contain authentic myomodulin. CBI-8/9 were found to receive synaptic input after mechanical stimulation of the tentacles. They also received excitatory input from C-PR, a neuron involved in neck lengthening, and received a slow inhibitory input from CC5, a cell involved in neck shortening, suggesting that CBI-8/9 may be active during forward movements of the head or buccal mass. Firing of CBI-8 or CBI-9 resulted in the activation of a relatively small number of buccal neurons as evidenced by extracellular recordings from buccal nerves. Firing also produced local movements of the buccal mass, in particular a strong contraction of the I7 muscle, which mediates radula opening. CBI-8/9 were found to produce a slow depolarization and rhythmic activity of B48, the motor neuron for the I7 muscle. The data provide continuing evidence that the small population of cerebral buccal interneurons is composed of neurons that are highly diverse in their functional roles. CBI-8/9 may function as a type of premotor neuron, or perhaps as a peptidergic modulatory neuron, the functions of which are dependent on the coactivity of other neurons.
Xin
Y
Y
Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York City 10032, USA.
Hurwitz
I
I
Perrins
R
R
Evans
C G
CG
Alexeeva
V
V
Weiss
K R
KR
Kupfermann
I
I
D000818:Animals; D001048:Aplysia; D002490:Central Nervous System / Q000502:physiology*; D017952:Ganglia, Invertebrate / Q000737:chemistry / Q000502:physiology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007395:Interneurons / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D007546:Isoquinolines / Q000032:analysis; D008239:Lysine / Q000031:analogs & derivatives / Q000032:analysis; D008564:Membrane Potentials / Q000502:physiology; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology; D009479:Neuropeptides / Q000378:metabolism*; D010507:Periodicity; D009435:Synaptic Transmission / Q000502:physiology
D007546:Isoquinolines; D009479:Neuropeptides; C053302:myomodulin; C017475:lucifer yellow; C013411:biocytin; D008239:Lysine