Role of the Trichoderma harzianum endochitinase gene, ech42, in mycoparasitism.
The role of the Trichoderma harzianum endochitinase (Ech42) in mycoparasitism was studied by genetically manipulating the gene that encodes Ech42, ech42. We constructed several transgenic T. harzianum strains carrying multiple copies of ech42 and the corresponding gene disruptants. The level of extracellular endochitinase activity when T. harzianum was grown under inducing conditions increased up to 42-fold in multicopy strains as compared with the wild type, whereas gene disruptants exhibited practically no activity. The densities of chitin labeling of Rhizoctonia solani cell walls, after interactions with gene disruptants were not statistically significantly different than the density of chitin labeling after interactions with the wild type. Finally, no major differences in the efficacies of the strains generated as biocontrol agents against R. solani or Sclerotium rolfsii were observed in greenhouse experiments.
Centro de Investigación y Estudios Avanzados, Plant Biotechnology and Genetic Engineering Unit, Irapuato, México.
C
C
Carsolio
N
N
Benhamou
S
S
Haran
C
C
Cortés
A
A
Gutiérrez
I
I
Chet
A
A
Herrera-Estrella
D015153:Blotting, Western; D002473:Cell Wall / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D002688:Chitinases / Q000235:genetics* / Q000378:metabolism; D004271:DNA, Fungal / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D017353:Gene Deletion; D005800:Genes, Fungal; D008854:Microscopy, Electron; D010572:Pest Control, Biological; D012232:Rhizoctonia / Q000254:growth & development; D012988:Soil Microbiology; D014170:Transformation, Genetic; D019076:Transgenes; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics / Q000502:physiology*
D004271:DNA, Fungal; D002686:Chitin; D002688:Chitinases