Syntaxin 11 is associated with SNAP-23 on late endosomes and the trans-Golgi network.
SNARE proteins are known to play a role in regulating intracellular protein transport between donor and target membranes. This docking and fusion process involves the interaction of specific vesicle-SNAREs (e.g. VAMP) with specific cognate target-SNAREs (e.g. syntaxin and SNAP-23). Using human SNAP-23 as the bait in a yeast two-hybrid screen of a human B-lymphocyte cDNA library, we have identified the 287-amino-acid SNARE protein syntaxin 11. Like other syntaxin family members, syntaxin 11 binds to the SNARE proteins VAMP and SNAP-23 in vitro and also exists in a complex with SNAP-23 in transfected HeLa cells and in native human B lymphocytes. Unlike other syntaxin family members, no obvious transmembrane domain is present in syntaxin 11. Nevertheless, syntaxin 11 is predominantly membrane-associated and colocalizes with the mannose 6-phosphate receptor on late endosomes and the trans-Golgi network. These data suggest that syntaxin 11 is a SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network in mammalian cells.
Valdez
A C
AC
Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Cabaniols
J P
JP
Brown
M J
MJ
Roche
P A
PA
D017398:Alternative Splicing; D001402:B-Lymphocytes; D002352:Carrier Proteins / Q000032:analysis* / Q000096:biosynthesis; D002458:Cell Fractionation; D002460:Cell Line; D003001:Cloning, Molecular; D011992:Endosomes / Q000648:ultrastructure*; D005260:Female; D015723:Gene Library; D006056:Golgi Apparatus / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000648:ultrastructure; D008565:Membrane Proteins / Q000032:analysis* / Q000096:biosynthesis / Q000235:genetics*; D009928:Organ Specificity; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D011994:Recombinant Proteins / Q000096:biosynthesis; D014158:Transcription, Genetic
D002352:Carrier Proteins; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D011994:Recombinant Proteins; C100493:SNAP23 protein, human