Simultaneous on-line extraction and analysis of sirolimus (rapamycin) and ciclosporin in blood by liquid chromatography-electrospray mass spectrometry.
We developed a sensitive and specific semi-automated liquid chromatography-electrospray mass spectrometric (HPLC-ESI-MS) assay for the simultaneous quantification of sirolimus and ciclosporin in blood. Following a simple protein precipitation step, the supernatants were injected into the HPLC system and extracted on-line. After column switching, the analytes were backflushed from the extraction column onto the analytical narrow-bore column and eluted into the ESI-MS system. The assay was linear from 0.4 to 100 microg/l sirolimus and from 2 to 1500 microg/l ciclosporin. The mean recoveries of sirolimus and ciclosporin were 98 and 96%, respectively. The mean interday precision/accuracy was 8.6%/-4.8% for sirolimus and 9.3%/-2.9% for ciclosporin.
Kirchner
G I
GI
Institut für Allgemeine Pharmakologie, Medizinische Hochschule Hannover, Germany. kirchner.gabriele@mh-hannover.de
Vidal
C
C
Jacobsen
W
W
Franzke
A
A
Hallensleben
K
K
Christians
U
U
Sewing
K F
KF
D002851:Chromatography, High Pressure Liquid; D016572:Cyclosporine / Q000097:blood*; D006801:Humans; D007166:Immunosuppressive Agents / Q000097:blood*; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D020123:Sirolimus / Q000097:blood*
D007166:Immunosuppressive Agents; D007202:Indicators and Reagents; D016572:Cyclosporine; D020123:Sirolimus