Anesthetic-induced structural changes in the nicotinic acetylcholine receptor.
The difference between infrared spectra of the nicotinic acetylcholine receptor (nAChR) recorded in the absence and presence of the agonist carbamylcholine (Carb) reveals a complex pattern of positive and negative bands that provides a spectral map of Carb-induced structural change. This spectral map is affected by the presence of either the local anesthetic, dibucaine, or the short chain alcohol, propanol. Both antagonists alter the intensities of difference bands in a manner consistent with the stabilization of a desensitized state. Spectral variations are also observed that are indicative of both the displacement of the anesthetics from the nAChR upon the addition of Carb and physical interactions that occur between the anesthetics and binding site residues.
Department of Biochemistry, University of Ottawa, Ontario, Canada.
S E
SE
Ryan
H P
HP
Nguyen
J E
JE
Baenziger
D000777:Anesthetics / Q000494:pharmacology*; D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D004557:Electric Organ / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D010277:Parasympathomimetics / Q000494:pharmacology; D011487:Protein Conformation / Q000187:drug effects; D011978:Receptors, Nicotinic / Q000737:chemistry* / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D014101:Torpedo / Q000378:metabolism*
D000777:Anesthetics; D010277:Parasympathomimetics; D011978:Receptors, Nicotinic; D002217:Carbachol