A method for quantifying differential expansion within hydrating hydrophilic matrixes by tracking embedded fluorescent microspheres.
A method is described for quantifying the pattern of deformation within a matrix and is demonstrated by analyzing the expansion of polymer hydrophilic matrix tablets. The fundamental features of the method are the incorporation of nondiffusing markers into the matrix and the subsequent tracking of these markers during deformation. Since the markers are too large to diffuse, their individual movement reflects the translocation of the surrounding matrix, and the separation between pairs of markers reveals any perturbation in the intervening area. By tracking many markers, the pattern of deformation within a matrix can be ascertained. The method was demonstrated on hydrating hydrophilic matrix tablets, using fluorescent microspheres as nondiffusing markers which were observed with a confocal laser scanning microscope. Analysis of the tracks showed a wave of expansion moving from the exterior toward the core, with the greatest and earliest expansion found in the outer regions. The results also showed that even as deeper layers started to expand the outer layers continued to swell.
School of Pharmaceutical Sciences, University of Nottingham, Nottingham NG7 2RD, UK.
J
J
Adler
A
A
Jayan
C D
CD
Melia
D002626:Chemistry, Pharmaceutical / Q000379:methods*; D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D065347:Hypromellose Derivatives; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000737:chemistry; D018613:Microscopy, Confocal; D008863:Microspheres; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D013607:Tablets / Q000737:chemistry*
D003692:Delayed-Action Preparations; D005456:Fluorescent Dyes; D011135:Polysaccharides, Bacterial; D013607:Tablets; D065347:Hypromellose Derivatives; D008747:Methylcellulose; C002563:xanthan gum