Antiangiogenic activity of restin, NC10 domain of human collagen XV: comparison to endostatin.
Based on a homology search with endostatin, the C-terminus 185 aa of collagen XVIII, we report the cloning, expression, and antiangiogenic activity of a 22 kDa human collagen XV fragment, that we have named restin. Restin was expressed in the prokaryotic pET expression system. We have shown that restin inhibits the migration of endothelial cells in vitro but has no effect on the proliferation of these cells. A polyclonal antibody raised against endostatin cross-reacted with restin. Systemic administration of restin suppressed the growth of tumors in a xenograft renal carcinoma model.
Renal Division, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
R
R
Ramchandran
M
M
Dhanabal
R
R
Volk
M J
MJ
Waterman
M
M
Segal
H
H
Lu
B
B
Knebelmann
V P
VP
Sukhatme
D000595:Amino Acid Sequence; D000970:Antineoplastic Agents / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D003001:Cloning, Molecular; D003094:Collagen / Q000737:chemistry* / Q000494:pharmacology*; D043170:Collagen Type XVIII; D043169:Endostatins; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000494:pharmacology; D008869:Microtubule-Associated Proteins; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000235:genetics* / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D010446:Peptide Fragments / Q000235:genetics* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics / Q000494:pharmacology; D017386:Sequence Homology, Amino Acid; D014183:Transplantation, Heterologous / Q000276:immunology
D000970:Antineoplastic Agents; D043170:Collagen Type XVIII; D043169:Endostatins; D007381:Intermediate Filament Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D010446:Peptide Fragments; D011994:Recombinant Proteins; C076744:cytoplasmic linker protein 170; D003094:Collagen