Herpes simplex virus type 1 inhibits in vitro differentiation and selected functions of human blood-derived monocytes.
We have studied the effect of herpes simplex virus type 1 (HSV1) infection on in vitro differentiation of blood-derived human monocytes into macrophages using morphological, functional and biochemical parameters that alter during macrophage differentiation. Purified preparations of HSV modified the monocyte-macrophage differentiation, in spite of the fact that the virus did not replicate in monocytes. Disappearance of expression of a monocyte-specific surface antigen and the typical development of morphological appearance were delayed in HSV- infected cells. Production of the lysosomal enzyme acid phosphatase, which normally increases during differentiation, was also reduced in infected cells. Transcription of the oncogenes c-myc and c-fos, and the Hsp70 gene was modified in cells from some donors but not in other cell preparations. Possible mechanisms of these effects are discussed.
Bruun
T
T
Institute of Medical Microbiology, Rikshospitalet, University of Oslo, Norway.
Kristoffersen
A K
AK
Rollag
H
H
Beck
S
S
Degré
M
M
D000135:Acid Phosphatase / Q000097:blood; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D016762:Genes, fos; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D007958:Leukocyte Count; D009000:Monocytes / Q000502:physiology*; D014158:Transcription, Genetic
D018840:HSP70 Heat-Shock Proteins; D000135:Acid Phosphatase