Platelet adhesion and complement activation studies on poly(N-alkyl mono and disubstituted) acrylamide derivatives.
Poly(N-alkyl mono and disubstituted) acrylamide derivatives were synthesized from poly(acryloyl chloride) by monomer analogous reaction. The polymers were characterized by FTIR-ATR and GPC. The contact angle measurements were performed to evaluate hydrophobic/hydrophilic characters of these polymers. The N-alkyl substituents changed contact angle between 55 and 75 degrees. In vitro platelet adhesion studies showed that surfaces of poly(N-alkyl substituted) acrylamides are prone to adhere platelets. Platelet spreading was more on poly(N-benzyl-N-ethyl acrylamide) surfaces in comparison to that on poly(N-benzyl-N-propionic acid ethyl ester acrylamide) and poly(N-benzyl acrylamide) surfaces. As a result of modification of amino group with N-alkyl substituents, the activations of C3a and C5a complements were suppressed 9-20% and 5-6% of native poly(acrylamides), respectively.
National Institute for Advanced Interdisciplinary Research, Bionic Design Research Group, Tsukuba, Ibaraki, Japan.
R
R
Bahulekar
N
N
Tamura
S
S
Ito
M
M
Kodama
D000179:Acrylates / Q000138:chemical synthesis / Q000494:pharmacology; D000180:Acrylic Resins / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003167:Complement Activation / Q000187:drug effects*; D015926:Complement C3a / Q000378:metabolism; D015936:Complement C5a / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D010973:Platelet Adhesiveness / Q000187:drug effects*; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D011817:Rabbits; D017550:Spectroscopy, Fourier Transform Infrared; D000099166:Polyacrylamides
D000179:Acrylates; D000180:Acrylic Resins; D001672:Biocompatible Materials; D015926:Complement C3a; D015936:Complement C5a; D011108:Polymers; D000099166:Polyacrylamides; C026200:acryloyl chloride