Development of a competitive ELISA for detection of primates infected with monkey B virus (Herpesvirus simiae).
Two competitive ELISAs (C-ELISAs) are described that allow detection of antibodies against monkey B virus (BV, Cercopithecine herpesvirus 1). The assays utilize monoclonal antibodies (MABs) directed against the BV glycoprotein B (gB). Two of these MABs specifically recognize BV gB while a third MAB also reacts with the gB homologues of other primate alpha-herpesviruses (herpes simplexvirus-1, HSV-1: HSV-2; simian agent-8, SA8; and Herpesvirus papio-2, HVP2). A C-ELISA using the single cross-reactive MAB 3E8 allowed detection of host antibodies against HSV-1, HSV-2, SA8, HVP2 or BV, thus proving to be a sensitive assay for the detection of infection by any of these primate alpha-herpesviruses. The C-ELISA using BV-specific MABs was less sensitive but did allow some discrimination between infection by BV versus other alpha-herpesviruses. It was also shown that a C-ELISA using HVP2 as antigen and the cross-reactive MAB 3E8 was as sensitive for detection of BV antibody in macaque sera as an assay employing BV antigen. This test format allows detection of BV-infected primates without the biohazards associated with preparation and use of BV antigen.
Department of Biochemistry and Microbiology, College of Osteopathic Medicine, Oklahoma State University, Tulsa 74107-1898, USA. blewett@osuunx.ucc.okstate.edu
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D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000942:Antigens, Bacterial / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006071:Gorilla gorilla; D000882:Haplorhini; D006566:Herpesviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D006568:Herpesvirus 1, Cercopithecine / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D008251:Macaca; D008992:Monkey Diseases / Q000175:diagnosis* / Q000821:virology; D019974:Pan paniscus; D002679:Pan troglodytes; D010215:Papio; D012680:Sensitivity and Specificity; D014759:Viral Envelope Proteins / Q000276:immunology
D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D014759:Viral Envelope Proteins