In vitro effect of deoxynivalenol on the differentiation of human colonic cell lines Caco-2 and T84.
The effects of low concentrations of deoxynivalenol (DON) on structural and functional characteristics of human colonic adenocarcinoma cell lines Caco-2 and T84 were examined. Scanning electron microscopic (SEM) analysis of the apical surfaces of Caco-2 cells revealed reduction or abnormal formation of brush borders in the presence of 50, 100 and 200 ng/ml of DON. Monolayer integrity of Caco-2 and T84 cells was studied using cells which were cultured on permeable membranes. The transepithelial electrical resistance (TEER) of Caco-2 cells was significantly reduced at 50, 100 and 200 ng/ml of DON, significant increase in lucifer yellow (LY) permeability was also observed in these cells at 100 ng/ml of DON. The TEER of T84 cells was significantly reduced at 100 and 200 ng/ml of DON. LY permeability significantly increased at 200 ng/ml of DON in T84 cells. Enzyme activities in Caco-2 cells were also examined. Alkaline phosphatase activity was reduced from the 6th to 15th day of culture in the presence of 100 or 200 ng/ml of DON, whereas sucrase-isomaltase activity was significantly decreased by adding 50 or 100 ng/ml of DON for 15 or 20 days. Protein content was attenuated only by treatment with 200 ng/ml of DON throughout the experimental period. The results indicate that DON interferes with structural and functional characteristics of differentiation in enterocytes at low doses.
Kasuga
F
F
Department of Biomedical Food Research, National Institute of Infectious Diseases, Tokyo, Japan.
Hara-Kudo
Y
Y
Saito
N
N
Kumagai
S
S
Sugita-Konishi
Y
Y
D000469:Alkaline Phosphatase / Q000378:metabolism; D018938:Caco-2 Cells; D002454:Cell Differentiation / Q000187:drug effects*; D002463:Cell Membrane Permeability; D003110:Colonic Neoplasms; D017097:Electric Impedance; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D008855:Microscopy, Electron, Scanning; D009834:Oligo-1,6-Glucosidase / Q000378:metabolism; D013393:Sucrase / Q000378:metabolism; D014255:Trichothecenes / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D014255:Trichothecenes; D000469:Alkaline Phosphatase; D009834:Oligo-1,6-Glucosidase; D013393:Sucrase; C007262:deoxynivalenol