The amino terminal regions of proBNP and proANP oligomerise through leucine zipper-like coiled-coil motifs.
We provide the first report of unique leucine zipper-like coiled-coil sequence motifs at the amino terminus (N-) of proBrain Natriuretic Peptide (proBNP) and proAtrial Natriuretic Peptide (proANP). These motifs were highly conserved across most of the known natriuretic peptide sequences from different species. Consistent with computer based modelling predictions, size exclusion (SE) chromatography analysis confirmed human and ovine N-BNP, N-ANP and human proBNP in plasma extracts to elute as high molecular weight oligomers. Synthetic model peptides corresponding to the proposed leucine zipper-like coiled-coil regions of proBNP, proANP and their related N-terminal peptides were shown to be sufficient to induce oligomerisation when assessed on size exclusion HPLC. To our knowledge, this is the first report of circulating peptides that oligomerise through leucine zipper-like coiled-coil motifs, and adds a new dimension to the field of vasoactive peptide research.
Christchurch Cardioendocrine Research Group, Christchurch School of Medicine, University of Otago, New Zealand.
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Seidler
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Pemberton
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Yandle
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Espiner
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Nicholls
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Richards
D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D017124:Conserved Sequence; D004285:Dogs; D006801:Humans; D016350:Leucine Zippers; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain; D009419:Nerve Tissue Proteins / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D010446:Peptide Fragments / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D011489:Protein Denaturation; D011498:Protein Precursors / Q000097:blood / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D051381:Rats; D017386:Sequence Homology, Amino Acid; D012756:Sheep
D009419:Nerve Tissue Proteins; D010446:Peptide Fragments; D011498:Protein Precursors; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain