Fast separation of (poly)phenolic compounds from apples and pears by high-performance liquid chromatography with diode-array detection.
Polyphenolic compounds in apples and pears were analysed by HPLC on C18-modified silica. Gradient elution with phosphoric acid-methanol mixtures and phosphoric acid-acetonitrile mixtures gave complete separation of all polyphenolics of interest. The use of methanol as modifier was preferred because it provides a more rapid separation (20 min). Diode-array detection was used for the provisional identification of polyphenolic compounds not available as standards.
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Alcalá, Madrid, Spain.
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Escarpa
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González
D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids; D005638:Fruit / Q000737:chemistry*; D000432:Methanol; D010636:Phenols / Q000302:isolation & purification*; D010756:Phosphoric Acids; D011108:Polymers / Q000302:isolation & purification*; D059808:Polyphenols; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000097:Acetonitriles; D005419:Flavonoids; D010636:Phenols; D010756:Phosphoric Acids; D011108:Polymers; D059808:Polyphenols; C030242:phosphoric acid; D000432:Methanol; C032159:acetonitrile