Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis.
Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR and gel lane per sample. Both methods yield co-dominant markers, with presence and absence of the transposon insertion independently scorable, and both could in principle be applied to any transposable element in any plant species.
Department of Biochemistry, University of Dundee, UK. a.j.flavell@dundee.ac.uk
A J
AJ
Flavell
M R
MR
Knox
S R
SR
Pearce
T H
TH
Ellis
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D008969:Molecular Sequence Data; D018532:Pisum sativum / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018626:Retroelements / Q000235:genetics*
D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers; D018626:Retroelements