REDOR-determined distances from heterospins to clusters of 13C labels.
The use of rotational-echo double resonance NMR to measure distances from an observed tightly coupled cluster of 13C spins to a distant 15N, 31P, or 19F is practical if all homonuclear 13C-13C dipolar interactions are suppressed by multiple-pulse decoupling during heteronuclear dipolar evolution. This scheme is first calibrated by experiments performed on multiply labeled alanines and then applied in the measurement of 19F-13C distances in p-trifluoromethylphenyl [1,2-13C2]acetate.
Department of Chemistry, Washington University, St. Louis, Missouri, 63130, USA.
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J
Schaefer
D000409:Alanine / Q000737:chemistry*; D002247:Carbon Isotopes / Q000032:analysis; D005462:Fluorine Radioisotopes / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D009587:Nitrogen Isotopes / Q000032:analysis; D010759:Phosphorus Isotopes / Q000032:analysis; D013113:Spin Labels
D002247:Carbon Isotopes; D005462:Fluorine Radioisotopes; D009587:Nitrogen Isotopes; D010759:Phosphorus Isotopes; D013113:Spin Labels; D000409:Alanine