[PCR-monitoring of gastric juice obtained with the capsuled string for the evaluation of H. pylori eradication].
We have developed a highly sensitive semi-nested PCR assay, URA-PCR, for the detection of H. pylori ureA gene in gastric juice. The primers were designed according to nucleotide sequence analyses of clinical isolates. The PCR assay has higher sensitivity than conventional methods such as culture. Characteristics of culture-negative but PCR-positive patients were similar to those of culture-positive ones. The PCR assay, using gastric juice samples obtained with capsuled strings, detected 97% cases of relapsed infection within eight weeks after antimicrobial therapy.
Yoshida
H
H
Department of Gastroenterology, Faculty of Medicine, University of Tokyo.
Maeda
S
S
Ogura
K
K
D001944:Breath Tests; D005750:Gastric Juice / Q000382:microbiology; D005798:Genes, Bacterial; D016481:Helicobacter Infections / Q000175:diagnosis* / Q000188:drug therapy; D016480:Helicobacter pylori / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014510:Urease / Q000235:genetics
D014510:Urease