Low molecular mass protein patterns in mycobacterial culture filtrates and purified protein derivatives.
Mycobacterial polypeptides from 2 kDa to 14 kDa may be involved in host response to infection and be useful for new diagnostics and vaccines. Tris-tricine SDS-polyacrylamide gel electrophoresis separation of proteins in tuberculin purified protein derivative (PPD) and in 6-8-week culture filtrates of Mycobacterium tuberculosis, Mycobacterium bovis BCG, and other Mycobacterium species demonstrated as many as 10 low molecular mass bands. Common and distinct bands were observed among different species and PPD. These low molecular mass culture filtrate proteins may represent potential diagnostic reagents and vaccines for Mycobacterium tuberculosis.
Department of Medical and Research Technology, University of Maryland School of Medicine, Baltimore 21202, USA.
S S
SS
Rowland
J L
JL
Ruckert
P J
PJ
Cummings
D000942:Antigens, Bacterial / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008970:Molecular Weight; D009161:Mycobacterium / Q000737:chemistry* / Q000254:growth & development; D009163:Mycobacterium bovis / Q000737:chemistry / Q000254:growth & development; D009169:Mycobacterium tuberculosis / Q000737:chemistry / Q000254:growth & development; D014373:Tuberculin / Q000737:chemistry*
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D014373:Tuberculin