Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.
To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white fat may prevent development of obesity.
Institute of Physiology, Academy Sciences of the Czech Republic, Prague.
F
F
Baumruk
P
P
Flachs
M
M
Horáková
D
D
Floryk
J
J
Kopecký
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism; D006153:Guanosine Diphosphate / Q000378:metabolism; D007473:Ion Channels; D008564:Membrane Potentials / Q000235:genetics*; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D026901:Membrane Transport Proteins; D051379:Mice; D008822:Mice, Transgenic; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins; D009765:Obesity / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2
D002352:Carrier Proteins; D005227:Fatty Acids; D005456:Fluorescent Dyes; D007473:Ion Channels; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D024101:Mitochondrial Proteins; D011506:Proteins; C000604372:Ucp1 protein, mouse; C000604390:Ucp2 protein, mouse; D000071256:Uncoupling Protein 1; D000071246:Uncoupling Protein 2; D006153:Guanosine Diphosphate; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone