Genomic complexity among strains of the facultative photoheterotrophic bacterium Rhodobacter sphaeroides.
Pulsed-field gel electrophoresis following the use of rare cutting restriction endonucleases together with Southern hybridization, using markers distributed on chromosomes I and II of Rhodobacter sphaeroides 2.4.1, has been used to examine approximately 25 strains of R. sphaeroides in an effort to assess the occurrence of genome complexity in these strains. The results suggest that genome complexity is widespread and is accompanied by substantial genomic heterogeneity.
Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center Medical School, Houston, Texas 77030, USA.
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D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D010957:Plasmids; D015183:Restriction Mapping; D016354:Rhodobacter capsulatus / Q000235:genetics; D012242:Rhodobacter sphaeroides / Q000235:genetics*
D004269:DNA, Bacterial