Preparation of N2, N2,7-trimethylguanosine affinity columns.
2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG-binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.
Espuny
R
R
Centro de Investigación y Desarrollo, CSIC, Barcelona, Spain.
Bahia
D
D
Barretto Cicarelli
R M
RM
Codony
C
C
Khaouja
A
A
Aviñó
A M
AM
Eritja
R
R
Bach-Elias
M
M
D000818:Animals; D000906:Antibodies; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins / Q000302:isolation & purification*; D002467:Cell Nucleus / Q000737:chemistry; D002846:Chromatography, Affinity / Q000295:instrumentation / Q000379:methods*; D003593:Cytoplasm / Q000737:chemistry; D005656:Fungal Proteins / Q000302:isolation & purification; D006151:Guanosine / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D006367:HeLa Cells; D006433:Hemocyanins; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000302:isolation & purification*; D011817:Rabbits; D012441:Saccharomyces cerevisiae; D012685:Sepharose; D012709:Serum Albumin
D000906:Antibodies; D000911:Antibodies, Monoclonal; D002352:Carrier Proteins; D005656:Fungal Proteins; D009687:Nuclear Proteins; D012709:Serum Albumin; D006151:Guanosine; C017778:N(2),N(2),7-trimethylguanosine; D012685:Sepharose; D006433:Hemocyanins; C032808:keyhole-limpet hemocyanin