Killing kinetics of intracellular Afipia felis treated with amikacin.
Afipia felis is a facultative intracellular bacterium which multiplies in macrophages following inhibition of phagosome-lysosome (P-L) fusion. When A. felis-infected cells are incubated for 72 h with various antibiotics, only aminoglycosides are found to be bactericidal. We therefore studied the killing of intracellular A. felis by amikacin, and its relationship with the restoration of P-L fusion. Amikacin reduced the number of A. felis from 8.5 x 10(5) to 3.5 x 102 cfu/mL within 94 h. P-L fusion was restored after 30-40 h of incubation with amikacin. Both mechanisms may participate in the intracellular killing of bacteria.
Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Université de la Méditerranée, Marseille, France.
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H
Le Pocher
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P
Brouqui
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D
Raoult
D000583:Amikacin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D015169:Colony Count, Microbial; D006090:Gram-Negative Bacteria / Q000187:drug effects* / Q000254:growth & development; D016905:Gram-Negative Bacterial Infections; D008247:Lysosomes / Q000187:drug effects / Q000648:ultrastructure; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D010588:Phagosomes / Q000187:drug effects / Q000648:ultrastructure
D000900:Anti-Bacterial Agents; D000583:Amikacin