Abnormal distribution of mesangium-specific laminin in glomeruli of patients with idiopathic membranous nephropathy.
Using a monoclonal antibody, the distribution of the mesangium-specific laminin that binds only to the mesangium in normal kidney, was studied immunohistochemically in renal tissues obtained from patients with different glomerulonephritides including idiopathic membranous nephropathy. In minimal change nephrotic syndrome and IgA nephropathy, the mesangium-specific laminin staining was restricted to the mesangium, as is observed in the normal kidney. However, in patients with idiopathic membranous nephropathy, the monoclonal antibody against the mesangium-specific laminin reacted with the glomerular peripheral capillary walls including subepithelial protrusions or 'spikes', as well as with the mesangium. These results suggest that the quality of laminin produced in response to the challenge of immune deposits in the peripheral capillary walls may be different from that in the rest of the glomerular basement membrane in idiopathic membranous nephropathy.
Department of Medicine, Juntendo University School of Medicine, Tokyo, Japan.
S
S
Horikoshi
I
I
Ebihara
T
T
Nakamura
H
H
Koide
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D001485:Basement Membrane / Q000378:metabolism; D005920:Glomerular Mesangium / Q000378:metabolism*; D005922:Glomerulonephritis, IGA / Q000378:metabolism; D015432:Glomerulonephritis, Membranoproliferative / Q000378:metabolism; D015433:Glomerulonephritis, Membranous / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007678:Kidney Glomerulus / Q000378:metabolism; D007797:Laminin / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009402:Nephrosis, Lipoid / Q000378:metabolism; D051381:Rats; D014018:Tissue Distribution
D000911:Antibodies, Monoclonal; D007797:Laminin