Identification of major glycoconjugates from Mycobacterium bovis culture filtrate by biotin-hydrazide labeling.
To identify Mycobacterium bovis glycoproteins, carbohydrates present in a delipidized M. bovis culture filtrate protein extract were biotin-hydrazide labeled. 11 carbohydrate-containing protein with a molecular weight of 15-, 19-, 25-, 32-, 35-, 39-, 42-, 48-, 52-, 58-, and 62-kDa were detected. The 52- and 32-kDa protein were deglycosylated by endoglycosidase-F.
Depto. Biología Celular, Instituto Nal. Cardiología Ignacio Chávez, México, DF.
F
F
Massó
E
E
Varela
A
A
Páez
E
E
Zenteno
L F
LF
Montaño
D001426:Bacterial Proteins / Q000737:chemistry; D019921:Biotinylation; D002846:Chromatography, Affinity / Q000379:methods; D017077:Culture Media, Conditioned / Q000737:chemistry; D006001:Glycoconjugates / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry; D006031:Glycosylation; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism; D009163:Mycobacterium bovis / Q000737:chemistry*
D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; D006001:Glycoconjugates; D006023:Glycoproteins; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase