Mapping of the Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis.
Two expressed sequence tags were isolated from a porcine skeletal muscle cDNA library and identified as the putative partial cDNAs of the porcine Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes after sequencing and homology search. Results of analysis of a pig-rodent somatic cell hybrid panel by PCR allowed the assignments of ATP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5.
DIPROVAL-Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy.
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L
Fontanesi
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R
Davoli
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C
Zijlstra
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AA
Bosma
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Russo
D000818:Animals; D002874:Chromosome Mapping / Q000662:veterinary*; D002878:Chromosomes, Human, Pair 1; D002881:Chromosomes, Human, Pair 12; D020224:Expressed Sequence Tags; D006801:Humans; D018482:Muscle, Skeletal / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D000254:Sodium-Potassium-Exchanging ATPase / Q000235:genetics*; D013552:Swine / Q000235:genetics*
D010732:Phosphofructokinase-1; D000254:Sodium-Potassium-Exchanging ATPase