Inhibition of nucleoside diphosphate kinase in rat liver mitochondria by added 3'-azido-3'-deoxythymidine.
The effect of 3'-azido-3'-deoxythymidine on nucleoside diphosphate kinase of isolated rat liver mitochondria has been studied. This is done by monitoring the increase in the rate of oxygen uptake by nucleoside diphosphate (TDP, UDP, CDP or GDP) addition to mitochondria in state 4. It is shown that 3'-azido-3'-deoxythymidine inhibits the mitochondrial nucleoside diphosphate kinase in a competitive manner, with a Ki value of about 10 microM as measured for each tested nucleoside diphosphate. It is also shown that high concentrations of GDP prevent 3'-azido-3'-deoxythymidine inhibition of the nucleoside diphosphate kinase.
Dipartimento di Biochimica e Biologia Molecolare, Università di Bari, and Centro di Studi sui Mitocondri e Metabolismo Energetico, C.N.R., Italy. d.valenti@biologia.uniba.it
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D000818:Animals; D001278:Atractyloside / Q000031:analogs & derivatives / Q000494:pharmacology; D001667:Binding, Competitive; D003565:Cytidine Diphosphate / Q000493:pharmacokinetics; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006153:Guanosine Diphosphate / Q000493:pharmacokinetics; D007700:Kinetics; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009701:Nucleoside-Diphosphate Kinase / Q000037:antagonists & inhibitors*; D009840:Oligomycins / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D013942:Thymine Nucleotides / Q000493:pharmacokinetics; D014530:Uridine Diphosphate / Q000493:pharmacokinetics; D015215:Zidovudine / Q000494:pharmacology*
D004791:Enzyme Inhibitors; D009840:Oligomycins; D013942:Thymine Nucleotides; D006153:Guanosine Diphosphate; D001278:Atractyloside; C027923:thymidine 5'-diphosphate; D015215:Zidovudine; D014530:Uridine Diphosphate; D003565:Cytidine Diphosphate; D009701:Nucleoside-Diphosphate Kinase; C003853:carboxyatractyloside