Teichuronic acid operon of Bacillus subtilis 168.
Sequence analysis reveals that the Bacillus subtilis 168 tuaABCDEFGH operon encodes enzymes required for the polymerization of teichuronic acid as well as for the synthesis of one of its precursors, the UDP-glucuronate. Mutants deficient in any of the tua genes, grown in batch cultures under conditions of phosphate limitation, were characterized by reduced amounts of uronate in their cell walls. The teichuronic acid operon belongs to the Pho regulon, as phosphate limitation induces its transcription. Placing the tuaABCDEFGH operon under the control of the inducible Pspac promoter allowed its constitutive expression independently of the phosphate concentration in the medium; the level of uronic acid in cell walls was dependent on the concentration of the inducer. Apparently, owing to an interdependence between teichoic and teichuronic acid incorporation into the cell wall, in examined growth conditions, the balance between the two polymers is maintained in order to insure a constant level of the wall negative charge.
Institut de génétique et de biologie microbiennes, Université de Lausanne, Switzerland.
B
B
Soldo
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V
Lazarevic
M
M
Pagni
D
D
Karamata
D000116:Acetylgalactosamine / Q000502:physiology; D000117:Acetylglucosamine / Q000502:physiology; D001412:Bacillus subtilis / Q000235:genetics*; D001483:Base Sequence; D002473:Cell Wall / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005798:Genes, Bacterial; D008954:Models, Biological; D008969:Molecular Sequence Data; D009112:Muramic Acids / Q000494:pharmacology; D016296:Mutagenesis; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000494:pharmacology; D009876:Operon; D010710:Phosphates / Q000494:pharmacology; D010957:Plasmids; D017422:Sequence Analysis, DNA; D013997:Time Factors; D014574:Uronic Acids / Q000737:chemistry*; D001616:beta-Galactosidase / Q000032:analysis
D009112:Muramic Acids; D010710:Phosphates; D014574:Uronic Acids; C031651:N-acetylmuramic acid; C010788:teichuronic acid; D001616:beta-Galactosidase; D009238:N-Acetylmuramoyl-L-alanine Amidase; D000116:Acetylgalactosamine; D000117:Acetylglucosamine