Binding evaluation of Isoform 1 from Cratylia mollis lectin to human mammary tissues.
The phenomenon of altered carbohydrates in transformed cell surfaces has been studied through histochemical techniques using lectins. Specific binding patterns to normal and transformed mammary tissues were evaluated by Isoform 1 from Cratylia mollis lectin (Cra Iso 1). Protocols using a direct method, incubation of Cra Iso I conjugated to peroxidase (Cra Iso 1-Per) with mammary tissues, followed by diaminobenzidine and hydrogen peroxidase interaction, were performed. Neoplastic tissues, marked by Cra Iso 1, showed a higher intensity of staining than normal ones, in comparison with Canavalia ensiformis lectin, Concanavalin A (Con A), conjugated to peroxidase (Con A-Per). The assay with Cra Iso 1 also indicated a possible utilization of this lectin to characterize normal and transformed mammary cells.
Departamento de Bioquímica-CCB, Universidade Federal de Pernambuco, UFPE, Brasil.
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Figueredo-Silva
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D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism*; D018270:Carcinoma, Ductal, Breast / Q000378:metabolism; D016022:Case-Control Studies; D005069:Evaluation Studies as Topic; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D018226:Fibroadenoma / Q000378:metabolism; D005348:Fibrocystic Breast Disease / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000378:metabolism*; D008875:Middle Aged; D037121:Plant Lectins; D010946:Plants, Medicinal; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism*
D037102:Lectins; D037121:Plant Lectins; D020033:Protein Isoforms