Duplication of insertion element IS50 associated with Tn5 transposition in Azospirillum brasilense.
The characterization of a DNA fragment with a Tn5 insertion in a regulatory nif gene of Azospirillum brasilense is reported. Restriction endonuclease mapping, Southern hybridization with a Tn5 probe, and nucleotide sequencing revealed that IS50 had duplicated in Tn5. The duplication of an IS50 element suggests the occurrence of a replicative mechanism of transposition. A strategy, based on the bacterial ability of homologous recombination that was used to precisely eliminate Tn5 along with the duplicated IS50 element, is presented.
Laboratory of Bacterial Genetics, School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India. tripathianil@hotmail.com
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Tripathi
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Tripathi
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Ganguli
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Bazzicalupo
D015806:Azospirillum brasilense / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D009586:Nitrogen Fixation / Q000235:genetics; D011995:Recombination, Genetic; D015183:Restriction Mapping; D014169:Transformation, Bacterial
D004251:DNA Transposable Elements; D004269:DNA, Bacterial