N-linked oligosaccharides of vomeromodulin, a putative pheromone transporter in rat.
Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.
Mechref
Y
Y
Department of Chemistry, Indiana University, Bloomington 47405, USA.
Ma
W
W
Hao
G
G
Novotny
M V
MV
D000818:Animals; D001692:Biological Transport; D002236:Carbohydrate Conformation; D002352:Carrier Proteins / Q000737:chemistry* / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D036341:Intercellular Signaling Peptides and Proteins; D008297:Male; D026901:Membrane Transport Proteins; D009844:Oligosaccharides / Q000737:chemistry* / Q000378:metabolism; D010675:Pheromones / Q000737:chemistry* / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D012794:Sialic Acids / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002352:Carrier Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; C092201:LOC690507; D026901:Membrane Transport Proteins; D009844:Oligosaccharides; D010675:Pheromones; D011134:Polysaccharides; D012794:Sialic Acids; D006026:Glycoside Hydrolases