Detection of movement at the erythrocyte's edge by scanning phase contrast microscopy.
A time-dependent set of line scans across an erythrocyte was recorded by a phase contrast laser scanning microscope. A method for edge detection, based on a two-stage fitting procedure of the theoretical intensity distribution in a line scan of phase contrast image to the experimental one, is suggested. Time-resolved fluctuations of the human erythrocyte's edge were obtained, employing this method. The same procedure was carried out for a metallic strip yielding a test for the background noise level. The suggested method was applied in examining the effect of chemical crosslinking and thiol reduction on cell membrane fluctuations of human erythrocytes.
Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv, Israel.
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A
Bitler
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A
Barbul
R
R
Korenstein
D002465:Cell Movement; D004229:Dithiothreitol / Q000494:pharmacology; D004912:Erythrocytes / Q000502:physiology*; D006801:Humans; D008858:Microscopy, Phase-Contrast
D004229:Dithiothreitol