Identification of mechanosensitive ion channels in the cytoplasmic membrane of Corynebacterium glutamicum.
Patch-clamp experiments performed on membrane fragments of Corynebacterium glutamicum fused into giant liposomes revealed the presence of two different stretch-activated conductances, 600 to 700 pS and 1,200 to 1,400 pS in 0.1 M KCl, that exhibited the same characteristics in terms of kinetics, ion selectivity, and voltage dependence.
Ruffert
S
S
Institut für Biochemie, Universität zu Köln, 50674 Köln, Germany.
Berrier
C
C
Krämer
R
R
Ghazi
A
A
D001426:Bacterial Proteins / Q000502:physiology; D002462:Cell Membrane / Q000502:physiology; D003352:Corynebacterium / Q000502:physiology*; D007473:Ion Channels / Q000187:drug effects / Q000502:physiology*; D007700:Kinetics; D008081:Liposomes; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques; D011189:Potassium Chloride / Q000494:pharmacology; D013314:Stress, Mechanical
D001426:Bacterial Proteins; D007473:Ion Channels; D008081:Liposomes; D011189:Potassium Chloride