Galectin 1 modulates attachment, spreading and migration of cultured vascular smooth muscle cells via interactions with cellular receptors and components of extracellular matrix.
Galectin 1 (Gal-1), a lactose-binding lectin, is a component of vascular extracellular matrix and secreted by human vascular smooth muscle cells (SMCs). The purpose of this study was to investigate a possible role of Gal-1 in controlling adhesion and migration of cultured human vascular SMCs. Gal-1 co-localised with laminin and cellular fibronectin in extracellular matrix (ECM) secreted by cultured human vascular SMCs. Recombinant glutathione S-transferase (GST)-Gal-1 fusion protein bound to laminin and cellular fibronectin in ELISA. GST-Gal-1 inhibited SMC attachment to laminin via interactions with both SMCs and laminin. GST-Gal-1 inhibited SMC spreading on plastic or on laminin, but not on cellular fibronectin. GST-Gal-1 modulated SMC migration on laminin and inhibited migration on cellular fibronectin. GST-Gal-1 bound to several 35S-labelled proteins in SMC extracts including laminin and alpha1beta1 integrin, identified by depletion of SMC protein extracts with respective antibodies. We conclude that Gal-1 is able to modulate SMC attachment, spreading and migration via interactions with ECM proteins and alpha1beta1 integrin.
Moiseeva
E P
EP
Department of Medicine, Division of Cardiology, University of Leicester, Glenfield General Hospital, Leicester, UK. em9@leicester.ac.uk
Spring
E L
EL
Baron
J H
JH
de Bono
D P
DP
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D002448:Cell Adhesion; D002465:Cell Movement; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology*; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D037483:Galectin 1; D005982:Glutathione Transferase / Q000235:genetics; D006388:Hemagglutinins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D039222:Integrin alpha1beta1; D016023:Integrins / Q000378:metabolism; D007797:Laminin / Q000378:metabolism; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D010982:Platelet-Derived Growth Factor / Q000494:pharmacology; D011993:Recombinant Fusion Proteins
D000276:Adjuvants, Immunologic; D005353:Fibronectins; D037483:Galectin 1; D006388:Hemagglutinins; D039222:Integrin alpha1beta1; D016023:Integrins; D007797:Laminin; D010982:Platelet-Derived Growth Factor; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase