In vivo incidence of apoptosis evaluated with the TdT FragEL DNA fragmentation detection kit in cartilage and bone cells of the rat tibia.
Previous studies have shown the occurrence of cell death by apoptosis in cartilage and bone cells, and have suggested a functional relationship between bone growth and remodelling on one hand, and numbers of apoptotic cells on the other. At present, no in vivo studies are available on the frequency of the apoptotic process measured at one time and in one place using the cartilage and bone cells of single specimens. The aim of the present investigation was to measure the in vivo incidence of apoptosis in cartilage and bone cells of the upper epiphysis and secondary ossification metaphyseal bone of the tibia in normal young adult rats. Apoptotic cells were visualized with the terminal deoxynucleotidyl transferase (TdT) FragEL DNA fragmentation detection kit, which is analogous to the TdT-mediated nick end-labelling (TUNEL) method. In the growth cartilage, only a few TUNEL-positive terminal hypertrophic chondrocytes were found; they were 1.32 +/- 0.70% of the total hypertrophic chondrocytes counted along the chondro-osseous junction. There were only a few apoptotic osteoblastic cells and osteocytes (0.22 +/- 0.22% and 0.15 +/- 0.16% of total osteoblasts and osteocytes respectively). TUNEL-positive osteoclasts were 1.03 +/- 0.57% of the total of osteoclastic cells; they usually showed only one or two apoptotic nuclei. The total number of TUNEL-positive bone marrow cells were also counted (56.78 +/- 10.29/mm2 of bone marrow spaces). Our results confirm that apoptosis does occur in hypertrophic chondrocytes and bone cells, and show that its frequency is very low. However, chiefly because of its short lifespan, the frequency of apoptosis in cartilage and bone may be higher than that shown by the TUNEL method. The static estimate that can be obtained with this method might lead to misleading conclusions on the physiological significance of such a dynamic, rapid and asynchronous process, whose precise importance in bone growth and remodelling remains to be determined.
Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy.
G
G
Silvestrini
P
P
Mocetti
P
P
Ballanti
R
R
Di Grezia
E
E
Bonucci
D000818:Animals; D017209:Apoptosis; D016723:Bone Remodeling / Q000502:physiology*; D002358:Cartilage, Articular / Q000166:cytology*; D053938:DNA Fragmentation; D004253:DNA Nucleotidylexotransferase / Q000032:analysis*; D006132:Growth Plate / Q000166:cytology*; D006984:Hypertrophy; D020287:In Situ Nick-End Labeling; D007421:Intestine, Small / Q000166:cytology; D008297:Male; D010006:Osteoblasts / Q000166:cytology; D010010:Osteoclasts / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D011933:Reagent Kits, Diagnostic; D013977:Tibia / Q000166:cytology*
D011933:Reagent Kits, Diagnostic; D004253:DNA Nucleotidylexotransferase