Molecular structure of canine LINE-1 elements in canine transmissible venereal tumor.
We determined the 4251-bp sequence of open reading frame 2 (ORF2) of canine LINE-1 retroposon that encodes 1275 amino acids. The truncated LINE-1 inserts associated with transmissible venereal tumor (TVT) of dogs contained the 1378-bp LINE-1 insert (TVT-LINE) flanked by 10-bp direct repeats upstream to c-myc gene. The TVT-LINE elements were composed of 416 bp inverse sequences homologous to the complementary strand of the LINE-1, a 5-bp deletion and 962-bp sequences homologous to the 3' region of the LINE-1.
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Y
Y
Choi
N
N
Ishiguro
M
M
Shinagawa
C J
CJ
Kim
Y
Y
Okamoto
S
S
Minami
K
K
Ogihara
D000818:Animals; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000662:veterinary; D014685:Venereal Tumors, Veterinary / Q000235:genetics*