Herpes simplex virus type 1 inhibits in vitro differentiation and selected functions of human blood-derived monocytes.
We have studied the effect of herpes simplex virus type 1 (HSV1) infection on in vitro differentiation of blood-derived human monocytes into macrophages using morphological, functional and biochemical parameters that alter during macrophage differentiation. Purified preparations of HSV modified the monocyte-macrophage differentiation, in spite of the fact that the virus did not replicate in monocytes. Disappearance of expression of a monocyte-specific surface antigen and the typical development of morphological appearance were delayed in HSV- infected cells. Production of the lysosomal enzyme acid phosphatase, which normally increases during differentiation, was also reduced in infected cells. Transcription of the oncogenes c-myc and c-fos, and the Hsp70 gene was modified in cells from some donors but not in other cell preparations. Possible mechanisms of these effects are discussed.
Institute of Medical Microbiology, Rikshospitalet, University of Oslo, Norway.
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Bruun
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Kristoffersen
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Rollag
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Beck
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Degré
D000135:Acid Phosphatase / Q000097:blood; D002454:Cell Differentiation; D002470:Cell Survival; D002478:Cells, Cultured; D016762:Genes, fos; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018259:Herpesvirus 1, Human / Q000502:physiology*; D006801:Humans; D007958:Leukocyte Count; D009000:Monocytes / Q000502:physiology*; D014158:Transcription, Genetic
D018840:HSP70 Heat-Shock Proteins; D000135:Acid Phosphatase