The role of the sympathetic nervous system in the regulation of leptin synthesis in C57BL/6 mice.
The objectives of this study were to determine whether leptin synthesis is regulated by the sympathetic nervous system and if so whether beta-adrenergic receptors mediate this effect. We show that sympathetic blockade by reserpine increases leptin mRNA levels in brown but not white adipose tissue, while acute cold-exposure decreases leptin expression 10-fold in brown adipose tissue and 2-fold in white adipose tissue. The cold-induced reduction in leptin mRNA can be prevented by a combination of propranolol and SR 59230A but not by either antagonist alone, indicating that beta3-adrenergic receptors and classical beta1/beta2-adrenergic receptors both mediate responses to sympathetic stimulation. Circulating leptin levels reflect synthesis in white adipose tissue but not in brown adipose tissue.
Department of Pharmacology, Monash University, Clayton, Victoria, Australia. bronwyn.evans@med.monash.edu.au
B A
BA
Evans
L
L
Agar
R J
RJ
Summers
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D003080:Cold Temperature; D020738:Leptin; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011412:Propanolamines / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000235:genetics*; D022702:Receptors, Adrenergic, beta-3; D054411:Receptors, Leptin; D012110:Reserpine / Q000494:pharmacology; D013564:Sympathetic Nervous System / Q000378:metabolism*
C097869:3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate; D020738:Leptin; D011412:Propanolamines; D011506:Proteins; D012333:RNA, Messenger; D011943:Receptors, Adrenergic, beta; D022702:Receptors, Adrenergic, beta-3; D054411:Receptors, Leptin; C515447:leptin receptor, mouse; D012110:Reserpine; D011433:Propranolol