Dynamic interaction between soluble tubulin and C-terminal domains of N-methyl-D-aspartate receptor subunits.
The cytoplasmic C-terminal domains (CTs) of the NR1 and NR2 subunits of the NMDA receptor have been implicated in its anchoring to the subsynaptic cytoskeleton. Here, we used affinity chromatography with glutathione S-transferase-NR1-CT and -NR2B-CT fusion proteins to identify novel binding partner(s) of these NMDA receptor subunits. Upon incubation with rat brain cytosolic protein fraction, both NR1-CT and NR2B-CT, but not glutathione S-transferase, specifically bound tubulin. The respective fusion proteins also bound tubulin purified from brain, suggesting a direct interaction between the two binding partners. In tubulin polymerization assays, NR1-CT and NR2B-CT significantly decreased the rate of microtubule formation without destabilizing preformed microtubules. Moreover, only minor fractions of either fusion protein coprecipitated with the newly formed microtubules. Consistent with these findings, ultrastructural analysis of the newly formed microtubules revealed a limited association only with the CTs of the NR1 and NR2B. These data suggest a direct interaction of the NMDA receptor channel subunit CTs and tubulin dimers or soluble forms of tubulin. The efficient modulation of microtubule dynamics by the NR1 and NR2 cytoplasmic domains suggests a functional interaction of the receptor and the subsynaptic cytoskeletal network that may play a role during morphological adaptations, as observed during synaptogenesis and in adult CNS plasticity.
Department of Neurochemistry, Max Planck Institute for Brain Research, Frankfurt am Main, Germany.
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van Rossum
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Kuhse
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Betz
D000818:Animals; D002846:Chromatography, Affinity; D003593:Cytoplasm / Q000378:metabolism / Q000648:ultrastructure; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D008870:Microtubules / Q000378:metabolism / Q000648:ultrastructure; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012995:Solubility; D014404:Tubulin / Q000096:biosynthesis / Q000378:metabolism*
D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D005982:Glutathione Transferase