Observations on the effects of low frequency electromagnetic fields on cellular transcription in Drosophila larvae reared in field-free conditions.
Drosophila larvae reared inside a micro-metal box with an internal field strength 0.004 microT, were treated with a magnetic field of 50 Hz, 8 microT. for 20 min. Control experienced 0.004 microT. Cellular transcript levels were assessed using slot blots and quantified using a Phosphorimager. Blots were hybridised using probes against HSP 70a, Histone 1.9, and Copia. The low frequency EMFs very significantly decreased transcript levels, indicating that experimental responses may be influenced by previous exposure or lack of previous exposure.
Tipping
D R
DR
School of Biological Sciences, University of Birmingham, Edgbaston, UK.
Chapman
K E
KE
Birley
A J
AJ
Anderson
M
M
D000704:Analysis of Variance; D000818:Animals; D015342:DNA Probes; D004251:DNA Transposable Elements / Q000235:genetics / Q000528:radiation effects; D004331:Drosophila melanogaster / Q000235:genetics / Q000528:radiation effects; D004574:Electromagnetic Fields; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000528:radiation effects; D006657:Histones / Q000235:genetics / Q000528:radiation effects; D007091:Image Processing, Computer-Assisted; D015151:Immunoblotting; D019476:Insect Proteins / Q000235:genetics / Q000528:radiation effects; D007814:Larva / Q000235:genetics / Q000528:radiation effects; D016014:Linear Models; D009024:Morphogenesis / Q000235:genetics / Q000528:radiation effects; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic / Q000528:radiation effects*
D015342:DNA Probes; D004251:DNA Transposable Elements; D018840:HSP70 Heat-Shock Proteins; D006657:Histones; D019476:Insect Proteins