A gene upregulated in the acoustically damaged chick basilar papilla encodes a novel WD40 repeat protein.
The chick WDR1 gene is expressed at higher levels in the chick basilar papilla after acoustic overstimulation. The 3.3-kb WDR1 cDNA encodes a novel 67-kDa protein containing nine WD40 repeats, motifs that mediate protein-protein interactions. The predicted WDR1 protein has high sequence identity to WD40-repeat proteins in budding yeast (Saccharomyces cerevisiae), two slime molds (Dictyostelium discoideum and Physarum polycephalum), and the roundworm (Caenorhabditis elegans). The yeast and P. polycephalum proteins bind actin, suggesting that the novel chick protein may be an actin-binding protein. Sequence database comparisons identified mouse and human cDNAs with high sequence identity to the chick WDR1 cDNA. The mouse Wdr1 and human WDR1 proteins showed 95% sequence identity to each other and 86% identity to the chick WDR1 protein. Northern blot analysis of total RNA from the chick basilar papilla after noise trauma revealed increased levels of a 3.1-kb transcript in the lesioned area. The WDR1 gene was mapped to human chromosome 4, between 22 and 24 cM from the telomere of 4p.
Department of Otolaryngology/Head-Neck Surgery, University of Michigan, Ann Arbor, Michigan, 48109, USA.
H J
HJ
Adler
R S
RS
Winnicki
T W
TW
Gong
M I
MI
Lomax
D000199:Actins / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001489:Basilar Membrane / Q000378:metabolism*; D002645:Chickens / Q000235:genetics* / Q000502:physiology; D002894:Chromosomes, Human, Pair 4 / Q000235:genetics; D015870:Gene Expression; D006317:Hearing Loss, Noise-Induced / Q000235:genetics; D006801:Humans; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D015183:Restriction Mapping; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D000199:Actins; D008840:Microfilament Proteins; C118067:WDR1 protein, human