Disruption of six novel Saccharomyces cerevisiae genes reveals that YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine.
Six open reading frames (ORFs) from chromosome, VII, YGL131c, YGL129c, YGL128c, YGL125w, YGL124c and YGL121c, were disrupted by deletion cassettes with short flanking regions homologous to the target locus (SFH). YGL129c is necessary for growth in non-fermentable carbon sources, YGL128c for growth at low or high temperatures and YGL125w is implicated in the biosynthesis of methionine. With regard to the other ORFs, basic phenotypic analyses did not reveal any significant clues about their function.
Tizón
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Departamento de Biología Celular y Molecular, Universidad de La Coruña, Spain.
Rodríguez-Torres
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AM
Cerdán
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ME
D002244:Carbon / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D008715:Methionine / Q000096:biosynthesis*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D013696:Temperature; D014170:Transformation, Genetic
D002244:Carbon; D008715:Methionine