Cloning and characterization of two cytoplasmic dynein intermediate chain genes in mouse and human.
Cytoplasmic dynein is a large multisubunit microtubule-based motor protein, which mediates movement of numerous intracellular organelles. We report here the identification of the human homologue of cytoplasmic dynein intermediate chain 1 gene (DNCI1) located on human chromosome 7q21.3-q22.1. The mouse orthologue (Dnci1) was identified along with another highly related gene, Dnci2, and their RNA in situ expression patterns were examined during mouse embryogenesis. Dnci1 was found to have a highly restricted expression domain in the developing forebrain as well as the peripheral nervous system (PNS), while Dnci2 displayed a broad expression profile throughout the entire central nervous system and most of the PNS. A dynamic expression profile was also found for Dnci2 in the developing mouse limb bud. The data presented here provide a framework for the further analysis of the functional role of Dnci1 and Dnci2 in mouse and DNCI1 in human.
Crackower
M A
MA
Department of Molecular and Medical Genetics, University of Toronto, The Hospital for Sick Children, Ontario, Canada.
Sinasac
D S
DS
Xia
J
J
Motoyama
J
J
Prochazka
M
M
Rommens
J M
JM
Scherer
S W
SW
Tsui
L C
LC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D018244:Chromosomes, Artificial, Yeast; D004398:Dyneins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D004622:Embryo, Mammalian / Q000378:metabolism; D005314:Embryonic and Fetal Development; D006801:Humans; D017403:In Situ Hybridization; D018878:Limb Buds / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014018:Tissue Distribution
D004398:Dyneins