title: Increase in the production of interleukin-6, interleukin-10, and interleukin-12 by lipopolysaccharide-stimulated peritoneal macrophages from women with endometriosis.
abstract: OBJECTIVE
  To verify whether the peritoneal macrophage (PM) is activated in endometriosis.
  
  
  METHODS
  We examined the synthesis of nitric oxide (NO), total antioxidant, interleukin (IL)-6, IL-10, and IL-12 by cultured PMs, which were either unstimulated or stimulated with lipopolysaccharide (LPS), from women with endometriosis (early, n = 12; advanced, n = 11) or without endometriosis (n = 13).
  
  
  RESULTS
  After stimulation with 2 ng/mL LPS for 24 hr, PMs from women with early-stage endometriosis secreted more NO, IL-6, and IL-10 than the controls. Higher IL-12 levels were noted in women with advanced endometriosis when compared with the controls. After 2 ng/mL-LPS stimulation for 24 hr, we also detected higher total antioxidant levels in the advanced-endometriosis group than those in the early-endometriosis group.
  
  
  CONCLUSIONS
  The increased production of IL-6, IL-10, and IL-12 by stimulated PMs confirmed previous observations that the PM is the principle source of these cytokines in peritoneal fluid.
authors: Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University Hospital, National Taiwan University, Taipei.
authors: M Y
authors: MY
authors: Wu
authors: H N
authors: HN
authors: Ho
authors: S U
authors: SU
authors: Chen
authors: K H
authors: KH
authors: Chao
authors: C D
authors: CD
authors: Chen
authors: Y S
authors: YS
authors: Yang
mesh_terms: D004715:Endometriosis / Q000276:immunology*; D005260:Female; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D015850:Interleukin-6 / Q000096:biosynthesis; D007378:Interleukins / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008262:Macrophage Activation; D017737:Macrophages, Peritoneal / Q000276:immunology*
chemical_list: D015850:Interleukin-6; D007378:Interleukins; D008070:Lipopolysaccharides; D016753:Interleukin-10; D018664:Interleukin-12