Reconstitution of regulated exocytosis in cell-free systems: a critical appraisal.
Regulated exocytosis involves the tightly controlled fusion of a transport vesicle with the plasma membrane. It includes processes as diverse as the release of neurotransmitters from presynaptic nerve endings and the sperm-triggered deposition of a barrier preventing polyspermy in oocytes. Cell-free model systems have been developed for studying the biochemical events underlying exocytosis. They range from semi-intact permeabilized cells to the reconstitution of membrane fusion from isolated secretory vesicles and their target plasma membranes. Interest in such cell-free systems has recently been reinvigorated by new evidence suggesting that membrane fusion is mediated by a basic mechanism common to all intracellular fusion events. In this chapter, we review some of the literature in the light of these new developments and attempt to provide a critical discussion of the strengths and limitations of the various cell-free systems.
Avery
J
J
Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany. rjahn@gwdg.de
Jahn
R
R
Edwardson
J M
JM
D000818:Animals; D002463:Cell Membrane Permeability; D002474:Cell-Free System; D005089:Exocytosis / Q000502:physiology*; D020558:GTP Phosphohydrolases / Q000502:physiology; D006801:Humans; D008561:Membrane Fusion; D010063:Ovum / Q000502:physiology; D012617:Sea Urchins / Q000502:physiology
D020558:GTP Phosphohydrolases