title: Phospholipase D1 in caveolae: regulation by protein kinase Calpha and caveolin-1.
abstract: Caveolae are small plasma membrane invaginations that have been implicated in cell signaling, and caveolin is a principal structural component of the caveolar membrane. Previously we have demonstrated that protein kinase Calpha (PKCalpha) directly interacts with phospholipase D1 (PLD1), activating the enzymatic activity of PLD1 in the presence of phorbol 12-myristate 13-acetate (PMA) [Lee, T. G., et al. (1997) Biochim. Biophys. Acta 1347, 199-204]. In this study, using a detergent-free procedure for the purification of a caveolin-enriched membrane fraction (CEM) and immunoblot analysis, we show that PLD1 is enriched in the CEMs of 3Y1 rat fibroblasts. Purified PLD1 directly bound to a glutathione S-transferase-caveolin-1 fusion protein in in vitro binding assays. The association of PLD1 with caveolin-1 could be completely eliminated by preincubation of PLD1 with an oligopeptide corresponding to the scaffolding domain (amino acids 82-101) of caveolin-1, indicating that caveolin-1 interacts with PLD1 through the scaffolding domain. The peptide also inhibited PKCalpha-stimulated PLD1 activity and the interaction between PLD1 and PKCalpha with an IC50 of 0.5 microM. PMA elicits translocation of PKCalpha to the CEMs, inducing PLD activation through the interaction of PKCalpha with PLD1 in the CEMs. Caveolin-1 also coimmunoprecipitated with PLD1 in the absence of PMA, and the amounts of coimmunoprecipitated caveolin-1 decreased in response to treatment with PMA. Taken together, our results suggest a new mechanism for the regulation of the PKCalpha-dependent PLD activity through the molecular interaction between PLD1, PKCalpha, and caveolin-1 in caveolae.
authors: Department of Life Science and School of Environmental Engineering, Pohang Univerisity of Science and Technology, Pohang, 790-784, Republic of Korea.
authors: J H
authors: JH
authors: Kim
authors: J M
authors: JM
authors: Han
authors: S
authors: S
authors: Lee
authors: Y
authors: Y
authors: Kim
authors: T G
authors: TG
authors: Lee
authors: J B
authors: JB
authors: Park
authors: S D
authors: SD
authors: Lee
authors: P G
authors: PG
authors: Suh
authors: S H
authors: SH
authors: Ryu
mesh_terms: D000818:Animals; D019556:COS Cells; D051242:Caveolin 1; D022461:Caveolins; D002462:Cell Membrane / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D010739:Phospholipase D / Q000096:biosynthesis / Q000378:metabolism*; D011487:Protein Conformation; D011493:Protein Kinase C / Q000378:metabolism*; D051571:Protein Kinase C-alpha; D013347:Subcellular Fractions / Q000201:enzymology; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; D022461:Caveolins; D007527:Isoenzymes; D008565:Membrane Proteins; C496628:PRKCA protein, human; D011493:Protein Kinase C; D051571:Protein Kinase C-alpha; D010739:Phospholipase D; C106801:phospholipase D1; D013755:Tetradecanoylphorbol Acetate