The cellular distribution and kinase activity of the Cdk family member Pctaire1 in the adult mouse brain and testis suggest functions in differentiation.
Pctaire1, a member of the family of cyclin-dependent kinases, has been shown to be particularly abundantly expressed in differentiated tissues such as testis and brain. However, very little is known about the cellular and subcellular distribution and function of Pctaire1 protein(s), which is the focus of this study. We show that Pctaire1 encoded two major proteins of M(r) approximately 62,000 and approximately 68,000, found predominantly in testis and brain. Within these two tissues, Pctaire1 was most abundant in the cytoplasm of terminally differentiated cells, notably, the pyramidal neurons in brain and elongated spermatids in testis. Immunoprecipitation experiments further showed that a kinase activity toward myelin basic protein was associated with Pctaire1 in the adult testis and brain and that its activity was potentially regulated through association with regulatory partner(s). These results suggest that Pctaire1 kinase might have an important role in differentiated cells such as postmitotic neurons and spermatogenic cells.
Besset
V
V
Department of Genetics, Columbia University College of Physicians and Surgeons, New York, New York 10032, USA.
Rhee
K
K
Wolgemuth
D J
DJ
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000033:anatomy & histology / Q000378:metabolism*; D019556:COS Cells; D002454:Cell Differentiation; D018844:Cyclin-Dependent Kinases; D015151:Immunoblotting; D017403:In Situ Hybridization; D008297:Male; D051379:Mice; D010770:Phosphotransferases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000276:immunology / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013737:Testis / Q000033:anatomy & histology / Q000378:metabolism*; D014018:Tissue Distribution
D012965:Sodium Chloride; D010770:Phosphotransferases; D017346:Protein Serine-Threonine Kinases; D018844:Cyclin-Dependent Kinases; C089463:PCTAIRE-1 protein kinase