title: The hxB gene, necessary for the post-translational activation of purine hydroxylases in Aspergillus nidulans, is independently controlled by the purine utilization and the nicotinate utilization transcriptional activating systems.
abstract: Molybdenum-containing enzymes of the hydroxylase class (such as xanthine dehydrogenase, aldehyde oxidase and nicotinate dehydrogenase) require a terminal sulphur atom attached to the molybdenum to hydroxylate their specific substrates. The transulphurylation reaction is carried out in Drosophila melanogaster by the product of the ma-I gene. In Aspergillus nidulans, the activity of the isofunctional and homologous HxB protein is needed in at least two different metabolic contexts, when the organism grows on purines and when it grows on nicotinate as nitrogen sources. We show here that the expression of the hxB gene is not constitutive. It is induced independently and additively by the inducers of the purine and of the nicotinate utilization pathways. Each of these induction pathways is affected independently by mutations in their cognate genes, uric acid induction by mutations in the UaY protein and nicotinate and 6-nicotinate induction by those in the hxnR/aplA complex. It is, in both metabolic contexts, exquisitely sensitive to nitrogen metabolite repression and highly dependent on the AreA GATA factor.
authors: Institut de Génétique et Microbiologie, Orsay, France.
authors: L
authors: L
authors: Amrani
authors: G
authors: G
authors: Cecchetto
authors: C
authors: C
authors: Scazzocchio
authors: A
authors: A
authors: Glatigny
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D001483:Base Sequence; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D006899:Mixed Function Oxygenases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009525:Niacin / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011499:Protein Processing, Post-Translational; D011687:Purines / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015533:Transcriptional Activation; D014527:Uric Acid / Q000378:metabolism
chemical_list: C079728:AreA protein, Aspergillus nidulans; D005656:Fungal Proteins; D011687:Purines; D014157:Transcription Factors; D009525:Niacin; D014527:Uric Acid; D006899:Mixed Function Oxygenases