Expression of lymphotoxin gene inserted into Theiler's murine encephalomyelitis virus.
Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non-fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as a vector for the introduction of a foreign gene into the central nervous system. Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme-linked immunosorbent assay on BHK-21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells.
Obuchi
M
M
Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
Odagiri
T
T
Lizuka
H
H
Ohara
Y
Y
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D015151:Immunoblotting; D008233:Lymphotoxin-alpha / Q000096:biosynthesis / Q000235:genetics* / Q000633:toxicity*; D051379:Mice; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000633:toxicity; D017945:Theilovirus / Q000235:genetics*; D014162:Transfection
D008233:Lymphotoxin-alpha; D011994:Recombinant Proteins