Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata).
We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDalpha and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
Kim
D U
DU
Department of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, South Korea.
Roh
T Y
TY
Lee
J
J
Noh
J Y
JY
Jang
Y J
YJ
Hoe
K L
KL
Yoo
H S
HS
Choi
M U
MU
D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment
D010739:Phospholipase D