title: A novel egg-derived tyrosine phosphatase, EDTP, that participates in the embryogenesis of Sarcophaga peregrina (flesh fly).
abstract: We have previously reported that cathepsin L mRNA is present in unfertilized eggs of Sarcophaga peregrina (flesh fly) as a maternal mRNA, which suggests that cathepsin L is required for embryogenesis. Now we have identified an egg protein, with a molecular mass of 100 kDa, that is extremely susceptible to cathepsin L digestion and which disappears rapidly as the embryos develop. We purified this protein to homogeneity, cloned its cDNA, and found that it contained a consensus sequence for the active site of tyrosine phosphatase. In fact this protein showed tyrosine phosphatase activity, indicating that it is a novel tyrosine phosphatase. The expression and subsequent disappearance of this protein, which we have named egg-derived tyrosine phosphatase (EDTP), may be indispensable for embryogenesis of Sarcophaga.
authors: Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
authors: S
authors: S
authors: Yamaguchi
authors: K
authors: K
authors: Homma
authors: S
authors: S
authors: Natori
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D056668:Cathepsin L; D002403:Cathepsins / Q000378:metabolism; D003001:Cloning, Molecular; D016384:Consensus Sequence / Q000235:genetics; D003546:Cysteine Endopeptidases; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D004175:Diptera / Q000196:embryology* / Q000201:enzymology; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000166:cytology; D047108:Embryonic Development; D010450:Endopeptidases; D005455:Fluorescent Antibody Technique; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007930:Leucine / Q000031:analogs & derivatives / Q000494:pharmacology; D008969:Molecular Sequence Data; D009865:Oocytes / Q000201:enzymology*; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D015853:Cysteine Proteinase Inhibitors; D004527:Egg Proteins; D019476:Insect Proteins; D012333:RNA, Messenger; D017027:Protein Tyrosine Phosphatases; D002403:Cathepsins; D010450:Endopeptidases; D003546:Cysteine Endopeptidases; D056668:Cathepsin L; D007930:Leucine; C024974:E 64