title: Ballistic transformation of Caenorhabditis elegans.
abstract: A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.
authors: Max Planck Institut für Biochemie, 82152, Martinsried, Germany.
authors: T
authors: T
authors: Wilm
authors: P
authors: P
authors: Demel
authors: H U
authors: HU
authors: Koop
authors: H
authors: H
authors: Schnabel
authors: R
authors: R
authors: Schnabel
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D019470:Biolistics; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003094:Collagen / Q000235:genetics; D006046:Gold; D006371:Helium; D015801:Helminth Proteins / Q000235:genetics; D008858:Microscopy, Phase-Contrast; D009154:Mutation / Q000235:genetics; D010316:Particle Size; D014170:Transformation, Genetic
chemical_list: D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C098282:rol-6 protein, C elegans; D006371:Helium; D006046:Gold; D003094:Collagen