title: Association of body cavity-based lymphoma and human herpesvirus 8 in an HIV-seronegative male. Report of a case with immunocytochemical and molecular studies.
abstract: BACKGROUND
  Recently lymphomas arising primarily in serosal surfaces have been found in patients with advanced acquired immunodeficiency syndrome (AIDS), but they very rarely seem to occur in human immunodeficiency virus (HIV)-negative patients. Studies on a subset of these lymphomas suggested that they represent a distinct entity associated with Kaposi's sarcoma-associated herpesvirus or human herpesvirus 8 (HHV-8).
  
  
  METHODS
  An 83-year-old, HIV-negative male was admitted to the hospital with a massive pleural effusion. Abdominal and chest computed tomographic scanning was normal. Cytologic analysis of the pleural effusion revealed a large cell, non-Hodgkin's lymphoma. Polymerase chain reaction analyses on genomic DNA from the pleural effusion demonstrated the presence of HHV-8 sequences in the absence of Epstein-Barr virus.
  
  
  CONCLUSIONS
  It is possible and advantageous to diagnose body cavity-based lymphoma with a combination of cytologic, immunocytochemical and molecular studies of the pleural effusion in conjunction with clinical and radiographic information.
authors: Department of Pathology, Hospital Universitario Ramón y Cajal, Madrid, Spain.
authors: P
authors: P
authors: San Miguel
authors: A
authors: A
authors: Manzanal
authors: R
authors: R
authors: García González
authors: C
authors: C
authors: Bellas
mesh_terms: D036541:ADP-ribosyl Cyclase; D051997:ADP-ribosyl Cyclase 1; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD; D000943:Antigens, Differentiation / Q000032:analysis; D015415:Biomarkers; D004396:Coloring Agents; D004279:DNA, Viral / Q000032:analysis; D004801:Eosine Yellowish-(YS); D018023:HIV Seronegativity; D006566:Herpesviridae Infections / Q000000981:diagnostic imaging / Q000473:pathology*; D019288:Herpesvirus 8, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007150:Immunohistochemistry; D008228:Lymphoma, Non-Hodgkin / Q000000981:diagnostic imaging / Q000473:pathology / Q000821:virology*; D008297:Male; D008562:Membrane Glycoproteins; D008751:Methylene Blue; D009244:NAD+ Nucleosidase / Q000032:analysis; D010996:Pleural Effusion / Q000000981:diagnostic imaging / Q000473:pathology / Q000821:virology; D014057:Tomography, X-Ray Computed
chemical_list: D015703:Antigens, CD; D000943:Antigens, Differentiation; D015415:Biomarkers; D004396:Coloring Agents; D004279:DNA, Viral; C043260:May-Grunwald Giemsa; D008562:Membrane Glycoproteins; D036541:ADP-ribosyl Cyclase; C498154:CD38 protein, human; D009244:NAD+ Nucleosidase; D051997:ADP-ribosyl Cyclase 1; D008751:Methylene Blue; D004801:Eosine Yellowish-(YS)