title: Optical recording of membrane potential on isolated spiral ganglion cells of newborn mice using a voltage-sensitive dye.
abstract: Optical imaging methods make monitoring of the membrane potential feasible. With this technique, one can observe different optical signals depending on optical properties, in response to membrane potential, using voltage-sensitive dyes. We used the multiple-site optical imaging system to investigate membrane potentials of the isolated, cultured spiral ganglion cells (SCGs) from newborn mice. We used a voltage-sensitive absorption dye. With high potassium (150 mM K+) exposure, the absorbency of SGCs stained with voltage-sensitive dye increased temporarily under a 700-nm interference filter at approximately 0.3%. This detection of the depolarization of SGCs using an optical recording technique with a voltage-sensitive dye is important because it shows that the simultaneous measurement of activity in a variety of regions is possible, as is the exploring of the intercellular signalling pathway.
authors: Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
authors: A
authors: A
authors: Matsumoto
authors: T
authors: T
authors: Doi
authors: M
authors: M
authors: Asako
authors: S M
authors: SM
authors: Yang
authors: T
authors: T
authors: Yamashita
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004396:Coloring Agents; D007091:Image Processing, Computer-Assisted; D008027:Light; D008564:Membrane Potentials / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D013136:Spiral Ganglion / Q000502:physiology*
chemical_list: D004396:Coloring Agents