title: Thymidine phosphorylase/platelet-derived endothelial cell growth factor is upregulated in advanced solid types of gastric cancer.
abstract: Previous studies demonstrated that the immunohistochemical expression of thymidine phosphorylase (dThdPase) was related with distant metastasis and disease progression. In this study we investigated the production of dThdPase/platelet-derived endothelial cell growth factor in gastric cancer quantitatively. In a total of 75 tumour tissues and 60 normal gastric mucosa specimens, dThdPase protein concentrations were determined by ELISA. The amount of dThdPase was significantly higher in the tumour tissue than in the normal tissue. Intratumoural dThdPase concentrations were significantly higher in Borrmann types I and II macroscopically, in poorly differentiated and solid type histologically, in the medullary type of the tumour stroma, and in the tumour-invading serosa. In the medullary type of the amount of tumour stroma, protein levels of dThdPase were positively correlated with the vertical diameter of the tumour (r = 0.580, P = 0.019). By immunohistochemical study, dThdPase expression on tumour cells was observed in all seven specimens with high dThdPase protein levels, but not in all 14 cases with low dThdPase protein levels (P < 0.05). In summary, these data indicated that dThdPase is up-regulated in advanced solid types of gastric cancer, suggesting that dThdPase production in carcinoma cells might be induced by the microenvironment.
authors: The Third Department of Surgery, Kanagawa Cancer Center, Yokohama City University, School of Medicine, Japan.
authors: T
authors: T
authors: Yoshikawa
authors: K
authors: K
authors: Suzuki
authors: O
authors: O
authors: Kobayashi
authors: M
authors: M
authors: Sairenji
authors: H
authors: H
authors: Motohashi
authors: A
authors: A
authors: Tsuburaya
authors: Y
authors: Y
authors: Nakamura
authors: A
authors: A
authors: Shimizu
authors: S
authors: S
authors: Yanoma
authors: Y
authors: Y
authors: Noguchi
mesh_terms: D000704:Analysis of Variance; D002277:Carcinoma / Q000201:enzymology* / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000473:pathology; D013939:Thymidine Phosphorylase / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D009363:Neoplasm Proteins; D013939:Thymidine Phosphorylase