[Telomere lengths and telomerase activity in liposarcomas].
We measured telomerase activity in 36 malignant and seven benign lipomatous neoplasias from 34 patients to assess the role of telomerase in the development of liposarcoma. The sensitive PCR-based telomerase assay (telomeric repeat amplification protocol-TRAP) was applied. We correlated telomerase activity with the shortening or elongation of telomeric repeat fragment length (TRF), measured by using hybridization with a telomere specific oligonucleotide probe. Telomerase activity was demonstrated in 69% of malignant tumors. This information may be helpful in distinguishing benign tumors from malignant neoplasias. Telomerase expression, however, seems to be characteristic of poorly differentiated liposarcomas. Telomerase activity was not correlated with age at the time of diagnosis or with sex. We observed that telomerase expressing tumors had higher proliferation indices than neoplasias lacking telomerase. Telomerase activity was observed in all eight recurrences, suggesting a close association of telomerase with the biologic behavior of liposarcomas. Therefore, we assume that telomerase plays a key role in the establishment and progression of lipomatous tumors.
Schneider-Stock
R
R
Institut für Pathologie, Otto-von-Guericke Universität Magdeburg. Regine.Schneider-Stock@medizin.uni-magdeburg.de
Eppler
C
C
Walter
H
H
Radig
K
K
Haeckel
C
C
Hoang-Vu
C
C
Epplen
J T
JT
Roessner
A
A
D006801:Humans; D008080:Liposarcoma / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*; D016615:Telomere / Q000235:genetics* / Q000648:ultrastructure
D015345:Oligonucleotide Probes; D019098:Telomerase