Effects of concanavalin A on protein-C activity.
Concanavalin A interacts specifically with the oligosaccharides from protein-C and modifies its anticoagulant activity. The lectin activates the protein-C activity in a dose dependent manner as demonstrated by in vitro and in vivo assays. Concanavalin A at low concentration (0.1 to 2 microg/mL) induces an increase on the catalytic activity of protein-C; at higher concentrations (5 to 20 microg/mL), the catalytic activity returns to the baseline. The effect of concanavalin A was prevented by incubating the protein-C with alpha-methyl-mannoside or by treating the purified protein-C with alpha-mannosidase; furthermore, cleavage of mannosidic residues diminishes its catalytic activity. Our results indicate that the oligomannosidic portion of protein-C participates in the regulation of the catalytic activity of this protein.
Martínez-Cruz
R
R
Unidad de Bioquímica e Inmunología, Instituto Tecnológico, Oaxaca, México.
Córdoba
F
F
Mireles-López
J C
JC
Zenteno
E
E
Pérez-Ortega
E
E
Martínez
M
M
Pérez-Campos
E
E
D000818:Animals; D002384:Catalysis / Q000187:drug effects; D003208:Concanavalin A / Q000009:adverse effects / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005337:Fibrin / Q000096:biosynthesis; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008361:Mannosidases / Q000378:metabolism; D008766:Methylmannosides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009844:Oligosaccharides / Q000378:metabolism; D010314:Partial Thromboplastin Time; D011486:Protein C / Q000302:isolation & purification / Q000378:metabolism*; D013918:Thrombin Time; D043323:alpha-Mannosidase
D037102:Lectins; D008766:Methylmannosides; D009844:Oligosaccharides; D011486:Protein C; D003208:Concanavalin A; C008466:methylmannoside; D005337:Fibrin; D008361:Mannosidases; D043323:alpha-Mannosidase