Alpha4 protein as a common regulator of type 2A-related serine/threonine protein phosphatases.
The catalytic activity of the C subunit of serine/threonine phosphatase 2A is regulated by the association with A (PR65) and B subunits. It has been reported that the alpha4 protein, a yeast homolog of the Tap42 protein, binds the C subunit of serine/threonine phosphatase 2A and protein phosphatase 2A-related protein phosphatases such as protein phosphatase 4 and protein phosphatase 6. In the present study, we showed that alpha4 binds these three phosphatases and the association of alpha4 reduces the activities of these phosphatases in vitro. In contrast, PR65 binds to the C subunit of serine/threonine phosphatase 2A but not to protein phosphatase 4 and protein phosphatase 6. These results suggest that the alpha4 protein is a common regulator of the C subunit of serine/threonine phosphatase 2A and protein phosphatase 2A-related protein phosphatases.
Nanahoshi
M
M
Biosignal Research Center, Kobe University, Japan.
Tsujishita
Y
Y
Tokunaga
C
C
Inui
S
S
Sakaguchi
N
N
Hara
K
K
Yonezawa
K
K
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D002460:Cell Line; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D010749:Phosphoprotein Phosphatases / Q000235:genetics* / Q000378:metabolism; D054648:Protein Phosphatase 2; D029701:Saccharomyces cerevisiae Proteins
D048868:Adaptor Proteins, Signal Transducing; D005656:Fungal Proteins; D029701:Saccharomyces cerevisiae Proteins; C100984:TAP42 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2