Genetic analysis of biodegradation of tetralin by a Sphingomonas strain.
A strain designated TFA which very efficiently utilizes tetralin has been isolated from the Rhine river. The strain has been identified as Sphingomonas macrogoltabidus, based on 16S rDNA sequence similarity. Genetic analysis of tetralin biodegradation has been performed by insertion mutagenesis and by physical analysis and analysis of complementation between the mutants. The genes involved in tetralin utilization are clustered in a region of 9 kb, comprising at least five genes grouped in two divergently transcribed operons.
Hernáez
M J
MJ
Departamento de Genética, Facultad de Biología, Universidad de Sevilla, E-41080 Seville, Spain.
Reineke
W
W
Santero
E
E
D001673:Biodegradation, Environmental; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005816:Genetic Complementation Test; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D020161:Physical Chromosome Mapping; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013764:Tetrahydronaphthalenes / Q000378:metabolism*; D014871:Water Microbiology
D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013764:Tetrahydronaphthalenes; C095210:tetralin