The effects of bovine respiratory syncytial on normal ovine lymphocyte responses to mitogens or antigens in vitro.
In the present study peripheral blod mononuclear cells (MNC) obtained from normal uninfected lambs were used to study the possible effects of bovine respiratory syncytial virus (BRSV) on lymphocyte responses to the mitogens, phytohaemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM) in vitro. Live BRSV had a depressive effect on the proliferative responses of normal MNC to PHA, Con A and PWM. Inactivated BRSV and a commercial preparation of prostaglandin E2 were also found to depress the proliferative responses of normal ovine MNC to PHA but recombinant tumour necrosis factor-alpha (TNF-alpha) had no such effect. Serum samples obtained from BRSV-infected lambs contained substances inhibitory to PHA-driven lymphocyte blastogenesis. Memory blastogenic responses to border disease virus (BDV) of lymyphocytes obtained from lambs previously primed with BDV were significantly reduced when lymphocytes were exposed to infectious BRSV.
Keles
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I
Department of Veterinary Clinical Science and Animal Husbandry, University of Liverpool, Leahurst, Neston, UK.
Sharma
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AK
Woldehiwet
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Z
Murray
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RD
D000818:Animals; D000956:Antigens, Viral / Q000494:pharmacology*; D018083:Border disease virus / Q000276:immunology; D002417:Cattle; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D015232:Dinoprostone / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology*; D018112:Respiratory Syncytial Virus, Bovine / Q000276:immunology*; D012756:Sheep; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
D000956:Antigens, Viral; D008934:Mitogens; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D014409:Tumor Necrosis Factor-alpha; D003208:Concanavalin A; D015232:Dinoprostone