In utero methylmercury exposure differentially affects the activities of selenoenzymes in the fetal mouse brain.
Pregnant ICR mice were subcutaneously injected with 0,5, or 3x3 mg Hg/kg of methylmercury (MeHg) on days 12,13, and 14(G12-14) of gestation and were sacrificed on G17. Activity of selenoenzymes, including glutathione peroxidase (GPx) and 5'- or 5-iodothyronine deiodinases (5'-DI, 5-DI), was determined in fetal brain and placenta. MeHg did not affect the concentration of Se in these tissues, while it significantly inhibited the activity of GPx in the fetal brain and placenta, but not in the maternal brain. Although the levels of thyroid hormones in the maternal and fetal plasma were not affected by MeHg, 5-DI decreased and 5'-DI increased in the fetal brain, as if they had responded to hypothyroidism. Because the level of T4 in the fetal plasma was not affected by MeHg, these changes in enzymatic activities may result in a harmful excess of T3 in the fetal brain. In addition, 5-DI activity was increased in the placenta of MeHg-treated mice. These effects of prenatal MeHg exposure on fetal and placental DIs differed from those of dietary-induced Se deficiency, where the activities of DIs were decreased or not affected. Further evaluation of the effect of MeHg on selenoenzymes, especially 5-DIs, is warranted.
Watanabe
C
C
Department of Environmental Health Sciences, Tohoku University Graduate School of Medicine, Seiryo-machi, Sendai, 980-8575, Japan. chiho@humeco.m.u-tokyo.ac.jp
Yoshida
K
K
Kasanuma
Y
Y
Kun
Y
Y
Satoh
H
H
D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000196:embryology / Q000201:enzymology; D001923:Brain Chemistry; D005260:Female; D005333:Fetus / Q000737:chemistry / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007453:Iodide Peroxidase / Q000378:metabolism*; D008628:Mercury / Q000032:analysis; D008767:Methylmercury Compounds / Q000493:pharmacokinetics / Q000633:toxicity*; D051379:Mice; D008813:Mice, Inbred ICR; D010920:Placenta / Q000737:chemistry / Q000187:drug effects; D011247:Pregnancy; D012643:Selenium / Q000032:analysis; D013963:Thyroid Hormones / Q000032:analysis / Q000097:blood; D000097564:Iodothyronine Deiodinase Type II
D005979:Glutathione Peroxidase; D007453:Iodide Peroxidase; D008628:Mercury; D008767:Methylmercury Compounds; D012643:Selenium; D013963:Thyroid Hormones; D000097564:Iodothyronine Deiodinase Type II; C117274:iodothyronine deiodinase type III