title: Isolation of multicopy suppressors of the calcium sensitivity of a mutant lacking the bZIP transcription factor Atf1 in fission yeast.
abstract: In Schizosaccharomyces pombe, recent studies have uncovered a set of putative transcription factors of the basic leucine zipper (bZIP) type (e.g., Atf1, Pcr1, Pap1), which function downstream of the Sty1 mitogen-activated protein kinase (MAPK) cascade which is involved in stress-activated signal transduction. Accordingly, a delta atf1 mutant is known to exhibit osmosensitivity for growth, since one of the targets of Atf1 is the gpd1+ gene, which is responsible for the osmoadaptive glycerol production mediated by the Sty1 MAPK cascade. During the course of our studies on the osmotic response in S. pombe, we found that growth of a delta atf1 mutant is highly sensitive to the level of Ca2+ ions in the medium (but less sensitive to Mg2+ and Na+ ions). This phenotype seemed to be relevant to the osmosensitivity, because an delta gpd1 mutant showed a similar phenotype. An attempt was therefore made to isolate multicopy suppressors of the calcium sensitivity exhibited by the delta atf1 cells. Among such suppressors were several bZIP factors, including two known proteins (Atf21 and Pcr1), and two new ones (named Atf31 and Zip1). These factors were characterized further, in comparison to Atf1, with special reference to the Sty1 MAPK signaling pathway.
authors: Laboratory of Molecular Microbiology, School of Agriculture, Nagoya University, Japan.
authors: R
authors: R
authors: Ohmiya
authors: C
authors: C
authors: Kato
authors: H
authors: H
authors: Yamada
authors: H
authors: H
authors: Aiba
authors: T
authors: T
authors: Mizuno
mesh_terms: D051697:Activating Transcription Factor 1; D000595:Amino Acid Sequence; D002118:Calcium; D002122:Calcium Chloride / Q000494:pharmacology; D003470:Culture Media / Q000494:pharmacology; D004268:DNA-Binding Proteins; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics; D016350:Leucine Zippers; D008969:Molecular Sequence Data; D016296:Mutagenesis; D000073718:Pancreatitis-Associated Proteins; D010641:Phenotype; D012568:Schizosaccharomyces / Q000187:drug effects / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D051697:Activating Transcription Factor 1; D003470:Culture Media; D004268:DNA-Binding Proteins; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; D014157:Transcription Factors; D005993:Glycerolphosphate Dehydrogenase; D002122:Calcium Chloride; D002118:Calcium