Analysis of SAS gene and CDK4 and MDM2 proteins in low-grade osteosarcoma.
The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.
Ragazzini
P
P
Laboratory of Oncologic Research, Rizzoli Orthopedic Institute, Bologna, Italy.
Gamberi
G
G
Benassi
M S
MS
Orlando
C
C
Sestini
R
R
Ferrari
C
C
Molendini
L
L
Sollazzo
M R
MR
Merli
M
M
Magagnoli
G
G
Bertoni
F
F
Bohling
T
T
Pazzagli
M
M
Picci
P
P
D000293:Adolescent; D000328:Adult; D002881:Chromosomes, Human, Pair 12; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000302:isolation & purification; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016133:Polymerase Chain Reaction; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051736:Proto-Oncogene Proteins c-mdm2; D059470:Tetraspanins
D004273:DNA, Neoplasm; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; C086385:TSPAN31 protein, human; D059470:Tetraspanins; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D018844:Cyclin-Dependent Kinases