Topology of recombinant rat upstream binding factor.
Transmission electron microscopy and single particle electron crystallography were employed to reconstruct high-quality projection images of a recombinant, acidic tail deficient form of rat upstream binding factor. The upstream binding factor was found to be dimeric and approximately 10 nm in diameter with a central region of low density. Distinct nodes were observable, of size and spacing consistent with being HMG boxes 3 and 4. The dimerisation domain seemed most probably to be located in the internal region of the structure.
Ridsdale
R A
RA
Department of Molecular Biology and Genetics, University of Guelph, ON, Canada.
Semotok
J L
JL
Larson
D E
DE
Rothblum
L I
LI
Harauz
G
G
D000818:Animals; D003461:Crystallography / Q000295:instrumentation / Q000379:methods; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D008958:Models, Molecular; D035463:Pol1 Transcription Initiation Complex Proteins; D011487:Protein Conformation; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D014157:Transcription Factors / Q000737:chemistry* / Q000648:ultrastructure
D004268:DNA-Binding Proteins; D035463:Pol1 Transcription Initiation Complex Proteins; D011994:Recombinant Proteins; D014157:Transcription Factors; C063844:transcription factor UBF