title: Iron reductase for magnetite synthesis in the magnetotactic bacterium Magnetospirillum magnetotacticum.
abstract: Ferric iron reductase was purified from magnetotactic bacterium Magnetospirillum (formerly Aquaspirillum) magnetotacticum (ATCC 31632) to an electrophoretically homogeneous state. The enzyme was loosely bound on the cytoplasmic face of the cytoplasmic membrane and was found more frequently in magnetic cells than in nonmagnetic cells. The molecular mass of the purified enzyme was calculated upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be about 36 kDa, almost the same as that calibrated by gel filtration analysis. The enzyme required NADH and flavin mononucleotide (FMN) as optimal electron donor and cofactor, respectively, and the activity was strongly inhibited by Zn2+ acting as a partial mixed-type inhibitor. The Km values for NADH and FMN were 4.3 and 0. 035 microM, respectively, and the Ki values for Zn2+ were 19.2 and 23.9 microM for NADH and FMN, respectively. When the bacterium was grown in the presence of ZnSO4, the magnetosome number in the cells and the ferric iron reductase activity declined in parallel with an increase in the ZnSO4 concentration of the medium, suggesting that the ferric iron reductase purified in the present study may participate in magnetite synthesis.
authors: Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-ku, Yokohama 226-8501, Japan.
authors: Y
authors: Y
authors: Noguchi
authors: T
authors: T
authors: Fujiwara
authors: K
authors: K
authors: Yoshimatsu
authors: Y
authors: Y
authors: Fukumori
mesh_terms: D038181:FMN Reductase; D005290:Ferric Compounds / Q000378:metabolism; D052203:Ferrosoferric Oxide; D007501:Iron / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010087:Oxides / Q000378:metabolism*; D012244:Rhodospirillaceae / Q000201:enzymology*
chemical_list: D005290:Ferric Compounds; D010087:Oxides; D007501:Iron; D038181:FMN Reductase; D009247:NADH, NADPH Oxidoreductases; C023903:ferric citrate iron reductase; D052203:Ferrosoferric Oxide