title: Probing the role of C-1 ester group in Naja naja phospholipase A2-phospholipid interactions using butanetriol-containing phosphatidylcholine analogues.
abstract: To understand the role of the ester moiety of the sn-1 acyl chain in phospholipase A2-glycerophospholipid interactions, we introduced an additional methylene residue between the glycerol C1 and C2 carbon atoms of phosphatidylcholines, and then studied the kinetics of hydrolysis and the binding of such butanetriol-containing phospholipids with Naja naja phospholipase A2. Hydrolysis was monitored by using phospholipids containing a NBD-labelled sn-2 acyl chain and binding was ascertained by measuring the protein tryptophan fluorescence. The hydrolysis of butanetriol-containing phospholipids was invariably slower than that of the glycerol-containing phospholipids. In addition, the enzyme binding with the substrate was markedly decreased upon replacing the glycerol residue with the 1,3,4-butanetriol moiety in phosphatidylcholines. These results have been interpreted to suggest that the sn-1 ester group in glycerophospholipids could play an important role in phospholipase A2-phospholipid interactions.
authors: Division of Membrane Biology, Central Drug Research Institute, Lucknow, India.
authors: V
authors: V
authors: Puri
authors: A
authors: A
authors: Arora
authors: C M
authors: CM
authors: Gupta
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000031:analogs & derivatives / Q000494:pharmacology; D000818:Animals; D000440:Butanols / Q000737:chemistry / Q000378:metabolism*; D017815:Elapidae / Q000378:metabolism*; D005453:Fluorescence; D006868:Hydrolysis; D007700:Kinetics; D008968:Molecular Conformation; D010713:Phosphatidylcholines / Q000737:chemistry / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism*; D011485:Protein Binding; D012910:Snake Venoms / Q000201:enzymology*; D014364:Tryptophan / Q000737:chemistry
chemical_list: D000440:Butanols; D010713:Phosphatidylcholines; D010743:Phospholipids; D012910:Snake Venoms; D015060:1,2-Dipalmitoylphosphatidylcholine; D014364:Tryptophan; D010741:Phospholipases A; D054467:Phospholipases A2; C114589:1,3,4-butanetriol