Ballistic transformation of Caenorhabditis elegans.
A novel method to transform the nematode Caenorhabditis elegans is described. DNA coprecipitated with gold particles is shot at worms by means of a helium beam. Transformed worms are either identified by a dominant visible marker or selected by a conditional lethal system.
Wilm
T
T
Max Planck Institut für Biochemie, 82152, Martinsried, Germany.
Demel
P
P
Koop
H U
HU
Schnabel
H
H
Schnabel
R
R
D000818:Animals; D030801:Animals, Genetically Modified; D019470:Biolistics; D017173:Caenorhabditis elegans / Q000235:genetics*; D029742:Caenorhabditis elegans Proteins; D003094:Collagen / Q000235:genetics; D006046:Gold; D006371:Helium; D015801:Helminth Proteins / Q000235:genetics; D008858:Microscopy, Phase-Contrast; D009154:Mutation / Q000235:genetics; D010316:Particle Size; D014170:Transformation, Genetic
D029742:Caenorhabditis elegans Proteins; D015801:Helminth Proteins; C098282:rol-6 protein, C elegans; D006371:Helium; D006046:Gold; D003094:Collagen