title: Rat embryo fibroblasts transformed by c-Jun display highly metastatic and angiogenic activities in vivo and deregulate gene expression of both angiogenic and antiangiogenic factors.
abstract: The comparative tumorigenicity in rats and nude mice of cell lines derived from FR3T3 and transformed by either c-jun, ras, SV40 lt, or bovine papilloma virus type 1 (BPV1) oncogenes was investigated. c-Jun-transformed cells were as tumorigenic and metastatic as Ras-transformed cells. Latencies were short, and numerous pulmonary metastases were observed in all injected animals. In contrast, tumors induced by s.c. injection of SV40-transformed cells developed slower, and none of the animals who received injections i.v. presented with metastases. BPV1-transformed cells had an intermediate tumorigenic and metastatic activity. Microvessels present in the different tumors were revealed by immunostaining with Griffonia (Bandeiraea) Simplicifolia lectin 1. Tumors obtained with c-Jun-transformed cells exhibited more neovascularization than those induced by the other oncogenes. By comparison to FR3T3 cells or SV40- or BPV1-transformed cells, c-Jun-transformed fibroblasts repress the antiangiogenic thrombospondin-1 and SPARC genes, whereas we found that they express higher levels of gene expression of the angiogenic vascular endothelial growth factor. Finally, as compared with cells before passage in animals, thrombospondin-1, SPARC, and VEGF gene expression was also deregulated in cell lines isolated from primary tumors induced by BPV1-transformants. Our results indicate that the high transforming potential of c-Jun, evidenced as soon as transformation is established in vitro, correlates with deregulation of gene expression of both angiogenic and antiangiogenic factors leading to rapid neovascularization of tumors.
authors: Université Paris XIII, UFR Léonard de Vinci, Laboratoire d'Oncologie cellulaire et d'imagerie des tumeurs, Bobigny, France.
authors: M
authors: M
authors: Kraemer
authors: R
authors: R
authors: Tournaire
authors: V
authors: V
authors: Dejong
authors: N
authors: N
authors: Montreau
authors: D
authors: D
authors: Briane
authors: C
authors: C
authors: Derbin
authors: B
authors: B
authors: Binétruy
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002461:Cell Line, Transformed; D002471:Cell Transformation, Neoplastic; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D016228:Endothelial Growth Factors / Q000378:metabolism; D005347:Fibroblasts / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D016758:Genes, jun; D011905:Genes, ras; D007150:Immunohistochemistry; D008222:Lymphokines / Q000378:metabolism; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics* / Q000473:pathology; D009389:Neovascularization, Pathologic; D015676:Osteonectin / Q000378:metabolism; D051381:Rats; D019700:Thrombospondin 1 / Q000378:metabolism; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D015676:Osteonectin; D019700:Thrombospondin 1; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D015500:Chloramphenicol O-Acetyltransferase; D019278:Matrix Metalloproteinase 3