Characterization of protein profiles and cross-reactivity of Blastocystis antigens by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis.
The protein profiles of Blastocystis hominis, B. lapemi, and B. ratti were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and species could be differentiated by this means as well as by Western-blot analysis with polyclonal antibodies. No intraspecies difference could be distinguished between the two B. hominis isolates or the three B. ratti isolates. Western-blot analysis showed extensive cross-reactivity of B. lapemi and B. hominis antigens with anti-B. ratti serum. Some of the cross-reactive antigens were glycoproteins as determined on the basis of their sensitivity to periodate treatment.
Chen
X Q
XQ
Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore.
Singh
M
M
Ho
L C
LC
Tan
S W
SW
Yap
E H
EH
D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D016844:Blastocystis / Q000737:chemistry* / Q000145:classification / Q000254:growth & development / Q000276:immunology; D016776:Blastocystis Infections / Q000469:parasitology*; D015153:Blotting, Western; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015800:Protozoan Proteins / Q000032:analysis*; D011817:Rabbits; D012967:Sodium Dodecyl Sulfate
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; D012967:Sodium Dodecyl Sulfate