Extraction of equine chorionic gonadotrophin from pregnant mare plasma by direct adsorption on chromatographic media.
Equine chorionic gonadotrophin (eCG) is a hormone of practical value in veterinary medicine and animal production. Here we report a novel preparation procedure based on its direct adsorption onto anionic-exchange resins in a batch-wise mode. The active plasma is previously conditioned to reduce pH and ionic strength to required levels. After the adsorption stage, a 90% recovery of the initial eCG is achieved, with a concentration factor of about 50 and an enrichment factor around 500, with high preservation of biological activity. Further purification is carried out by cation-exchange column chromatography. The recovery for the whole process is higher than 70%, and the final potency of the preparation is close to 4000 IU/mg. The process is well suited for its application to the industrial scale.
González
G
G
Cátedra de Inmunología, Facultad de Química, Montevideo, Uruguay.
Castro
B
B
Massaldi
H
H
D000327:Adsorption; D000818:Animals; D001709:Biotechnology; D002852:Chromatography, Ion Exchange; D005260:Female; D006064:Gonadotropins, Equine / Q000097:blood* / Q000302:isolation & purification*; D006736:Horses; D007475:Ion Exchange Resins; D011247:Pregnancy; D012997:Solvents
D006064:Gonadotropins, Equine; D007475:Ion Exchange Resins; D012997:Solvents