Interaction of tumor and normal blood cells with ethylene oxide and propylene oxide block copolymers.
Ethylene oxide and propylene oxide block copolymers (pluronics) are widely known as agents that promote drug penetration across biological barriers. We have studied the interaction of normal and malignant blood cells with pluronics L61 and P85 that have different hydrophobicity. SP2/0 myeloma cells accumulated pluronics while normal cells adsorb most of the polymer on the surface. Interaction of pluronics with cells resulted in drastic changes of membrane microviscosity. Tumor cell membrane microviscosity decreased after pluronics adsorption, in contrast to normal cells, whose membrane microviscosity was enhanced. We suppose that sensitivity of tumor cell membrane microviscosity to the pluronics action correlates with its permeability for molecular substances.
Melik-Nubarov
N S
NS
Department of Polymer Science, School of Chemistry, Moscow State University, Russia. melik-nubarov@mail.ru
Pomaz
O O
OO
Dorodnych TYu
Badun
G A
GA
Ksenofontov
A L
AL
Schemchukova
O B
OB
Arzhakov
S A
SA
D000818:Animals; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008214:Lymphocytes / Q000737:chemistry / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008560:Membrane Fluidity / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D009101:Multiple Myeloma / Q000097:blood; D020442:Poloxamer / Q000378:metabolism / Q000494:pharmacology*; D013501:Surface-Active Agents / Q000378:metabolism / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
D013501:Surface-Active Agents; D020442:Poloxamer