Lack of interleukin 10 regulation of antigen presentation-associated molecules expressed on colonic epithelial cells.
BACKGROUND
Colonic epithelial cells may behave as antigen-presenting cells. Interleukin 10 (IL-10) is known to play a major role in the intestinal immune system; however, it remains to be determined whether human intestinal epithelial cells express IL-10 receptor, and whether this cytokine modulates their expression of antigen presentation-associated molecules.


METHODS
The binding of biotinylated IL-10 was studied in SW 1116, HT-29 and T84 human colonic epithelial cell lines and freshly isolated normal colonic epithelial cells. Reverse transcription-polymerase chain reaction was also performed to detect IL-10 receptor mRNA. The effect of IL-10 on antigen presentation associated molecules was assessed by flow cytometry.


RESULTS
Biotinylated IL-10 bound to SW 1116, HT-29, T84, and normal colonic epithelial cells. IL-10 receptor mRNA was detected in SW 1116 and normal epithelial cells. SW 1116 and HT-29 cells expressed MHC class I and ICAM-1, but not CD80, and SW 1116 constitutively expressed HLA-DR. Interferon-gamma up-regulated HLA-DR and ICAM-1 expression on both cells, whereas lipopolysaccharide increased ICAM-1 expression only on SW 1116. IL-10 failed to modulate these antigens, even after stimulation by lipopolysaccharide or interferon-gamma. Moreover, these molecules decreased IL-10 binding in both lines.


CONCLUSIONS
The presence of IL-10 receptor on intestinal epithelial cells suggest that IL-10 may play a role in mucosal physiology, however its effect on the immune response remains to be determined.
Bourreille
A
A
Centre de Recherche en Nutrition Humaine, Nantes, France.
Segain
J P
JP
Raingeard de la Blétière
D
D
Siavoshian
S
S
Vallette
G
G
Galmiche
J P
JP
Blottière
H M
HM
D000230:Adenocarcinoma / Q000276:immunology; D017951:Antigen Presentation; D018122:B7-1 Antigen / Q000276:immunology; D002469:Cell Separation; D003106:Colon / Q000166:cytology / Q000276:immunology*; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology; D006684:HLA-DR Antigens / Q000276:immunology; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology*; D007413:Intestinal Mucosa / Q000166:cytology / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D008285:Major Histocompatibility Complex; D018123:Receptors, Interleukin / Q000302:isolation & purification*; D053701:Receptors, Interleukin-10; D014407:Tumor Cells, Cultured
D018122:B7-1 Antigen; D006684:HLA-DR Antigens; D015395:Histocompatibility Antigens Class I; D008070:Lipopolysaccharides; D018123:Receptors, Interleukin; D053701:Receptors, Interleukin-10; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D007371:Interferon-gamma