Cloning of the SmSPO-1 gene preferentially expressed in sporocyst during the life cycle of the parasitic helminth Schistosoma mansoni1.
Schistosomes are parasitic helminths with a complex life cycle in human and snail hosts. They express stage-specific genes that conceivably determine distinct properties of the parasite at different developmental stages. Here we report the stage-specific gene SmSPO-1, which is preferentially expressed in sporocysts residing in the snail host. The cDNA and the gene were cloned and sequenced. The cDNA, from cap site to the poly(A) addition site, is 498 bp long. It encodes a protein of 117 amino acids with a hydrophobic signal peptide of 18 residues, indicating that SmSPO-1 is a secreted or a membranal protein. In the gene the cDNA is split into four exons spread over 2.1 kb of chromosomal DNA.
Ram
D
D
Department of Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel.
Lantner
F
F
Ziv
E
E
Lardans
V
V
Schechter
I
I
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D017238:Genes, Helminth; D015801:Helminth Proteins / Q000235:genetics*; D008018:Life Cycle Stages; D008969:Molecular Sequence Data; D012550:Schistosoma mansoni / Q000235:genetics* / Q000378:metabolism; D012908:Snails / Q000469:parasitology*; D013170:Spores / Q000378:metabolism
D018076:DNA, Complementary; D015801:Helminth Proteins; C118969:SPO-1 protein, Schistosoma mansoni