Two constituents of the initiation complex of the mannan-binding lectin activation pathway of complement are encoded by a single structural gene.
Mannan-binding lectin (MBL) forms a multimolecular complex with at least two MBL-associated serine proteases, MASP-1 and MASP-2. This complex initiates the MBL pathway of complement activation by binding to carbohydrate structures present on bacteria, yeast, and viruses. MASP-1 and MASP-2 are composed of modular structural motifs similar to those of the C1q-associated serine proteases C1r and C1s. Another protein of 19 kDa with the same N-terminal sequence as the 76-kDa MASP-2 protein is consistently detected as part of the MBL/MASP complex. In this study, we present the primary structure of this novel MBL-associated plasma protein of 19 kDa, MAp19, and demonstrate that MAp19 and MASP-2 are encoded by two different mRNA species generated by alternative splicing/polyadenylation from one structural gene.
Stover
C M
CM
Department of Microbiology and Immunology, University of Leicester, United Kingdom.
Thiel
S
S
Thelen
M
M
Lynch
N J
NJ
Vorup-Jensen
T
T
Jensenius
J C
JC
Schwaeble
W J
WJ
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001798:Blood Proteins / Q000737:chemistry; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D037222:Collectins; D003167:Complement Activation / Q000235:genetics*; D005091:Exons; D005796:Genes / Q000276:immunology*; D006801:Humans; D007438:Introns; D037102:Lectins / Q000378:metabolism*; D008351:Mannans / Q000378:metabolism*; D050606:Mannose-Binding Protein-Associated Serine Proteases; D008969:Molecular Sequence Data; D008970:Molecular Weight; D012333:RNA, Messenger / Q000737:chemistry; D051381:Rats; D017422:Sequence Analysis, DNA; D012697:Serine Endopeptidases / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D014158:Transcription, Genetic / Q000276:immunology
D001798:Blood Proteins; D002352:Carrier Proteins; D037222:Collectins; D037102:Lectins; D008351:Mannans; D012333:RNA, Messenger; C105296:MASP2 protein, human; D050606:Mannose-Binding Protein-Associated Serine Proteases; D012697:Serine Endopeptidases