Influence of hydrodynamic conditions on naphthalene dissolution and subsequent biodegradation.
The influence of hydrodynamic conditions on the dissolution rate of crystalline naphthalene as a model polycyclic aromatic hydrocarbon (PAH) was studied in stirred batch reactors with varying impeller speeds. Mass transfer from naphthalene melts of different surface areas to the aqueous phase was measured and results were modeled according to the film theory. Results were generalized using dimensionless numbers (Reynolds, Schmidt, and Sherwood). In combined mass transfer and biodegradation experiments, the effect of hydrodynamic conditions on the degradation rate of naphthalene by Pseudomonas 8909N was studied. Experimental results were mathematically described using mass-transfer and microbiological models. The experiments allowed determination of mass-transfer and microbiological parameters separately in a single run. The biomass formation rate under mass transfer limited conditions, which is related to the naphthalene biodegradation rate, was correlated to the dimensionless Reynolds number, indicating increased bioavailability at increased mixing in the reactor liquid. The methodology presented in which mass transfer processes are quantified under sterile conditions followed by a biodegradation experiment can also be adapted to more complex and realistic systems, such as particulate, suspended PAH solids or soils with intrapartically sorbed contaminants when the appropriate mass-transfer equations are incorporated.
Mulder
H
H
Laboratory of Ecotoxicology, National Institute of Public Health and the Environment, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. eric.mulder@rivm.nl
Breure
A M
AM
Van Andel
J G
JG
Grotenhuis
J T
JT
Rulkens
W H
WH
D001673:Biodegradation, Environmental; D019149:Bioreactors; D001709:Biotechnology; D008954:Models, Biological; D009281:Naphthalenes / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism; D012995:Solubility
D009281:Naphthalenes; D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; C031721:naphthalene