title: Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence.
abstract: Groups of 4-week-old White Rock chickens were inoculated intraconjunctivally with nine isolates of Newcastle disease virus representing all pathotypes. Birds were monitored clinically and euthanatized sequentially, with collection of tissues for histopathologic examination and in situ hybridization using an anti-sense digoxigenin-labeled riboprobe corresponding to the sequence of the gene coding for the matrix protein. Disease was most severe with velogenic viscerotropic pathotypes and was characterized by acute systemic illness with extensive necrosis of lymphoid areas in the spleen and intestine. Viral nucleic acid was detected in multiple tissues but most prominently in macrophages associated with lymphoid tissue. Velogenic neurotropic isolates caused central nervous system disease despite minimal amounts of viral nucleic acid detected in neural tissue. Mesogenic and lentogenic pathotypes caused no overt disease; however, viral nucleic acid was present in myocardium and air sac epithelium following infection with these isolates. Compromise of air sac and myocardium may predispose mesogen- and lentogen-infected chickens to secondary infection and/or decreased meat and egg production.
authors: Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens 30602-7388, USA.
authors: C
authors: C
authors: Brown
authors: D J
authors: DJ
authors: King
authors: B S
authors: BS
authors: Seal
mesh_terms: D000400:Air Sacs / Q000473:pathology / Q000821:virology; D000818:Animals; D000911:Antibodies, Monoclonal; D001921:Brain / Q000473:pathology / Q000821:virology; D002432:Cecum / Q000473:pathology / Q000821:virology; D002642:Chick Embryo; D002645:Chickens; D006321:Heart / Q000821:virology; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000662:veterinary; D008221:Lymphoid Tissue / Q000473:pathology / Q000821:virology; D009206:Myocardium / Q000473:pathology; D009521:Newcastle Disease / Q000473:pathology / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000473:pathology / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal