title: Subunit assembly and domain analysis of electrically silent K+ channel alpha-subunits of the rat Kv9 subfamily.
abstract: Alpha-subunits of the voltage-gated potassium channel (Kv) subfamily Kv9 show no channel activity after homomultimeric expression in heterologous expression systems. This report shows that heteromultimeric expression of rKv9.1 and rKv9.3 specifically suppresses the currents mediated by alpha-subunits of the Kv2 and Kv3 subfamilies but does not affect currents mediated by alpha-subunits of the Kv1 and Kv4 subfamilies. To understand the molecular basis of the electrical silence of Kv9 homomultimeric channels, crucial functional domains (amino and carboxy terminus, S4 segment, and pore region) were exchanged between Kv9 alpha-subunits and rKv1.3. Electrophysiological studies of these chimeras revealed that the pore region is involved in determining the nonconductive behavior of homomultimeric Kv9 channels. This analysis was extended by protein interaction assays, aiming to identify the region of Kv9 subunits responsible for the specific suppression of rKv2.1- and rKv3.4-mediated currents. We could show that the amino-terminal domain of Kv9 alpha-subunits does not support homomultimeric assembly but interacts specifically with the rKv2.1 amino-terminal region. Conversely, the specific intersubfamily assembly of rKv3.4 with rKv9.1 or rKv9.3 is governed by the hydrophobic core and not the amino-terminal domain.
authors: Molekulare Biologie Neuronaler Signale, Max-Planck-Institut für experimentelle Medizin, Göttingen, Germany.
authors: M
authors: M
authors: Stocker
authors: M
authors: M
authors: Hellwig
authors: D
authors: D
authors: Kerschensteiner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D018076:DNA, Complementary; D051668:Delayed Rectifier Potassium Channels; D015870:Gene Expression / Q000502:physiology; D015640:Ion Channel Gating / Q000502:physiology*; D008969:Molecular Sequence Data; D010957:Plasmids; D015221:Potassium Channels / Q000737:chemistry* / Q000235:genetics*; D024642:Potassium Channels, Voltage-Gated; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics; D051669:Shab Potassium Channels; D051670:Shaw Potassium Channels; D029867:Xenopus Proteins; D014982:Xenopus laevis; D015003:Yeasts / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D051668:Delayed Rectifier Potassium Channels; C492016:KCNC4 protein, Xenopus; C492014:Kcnc4 protein, rat; C503465:Kcns1 protein, rat; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D011993:Recombinant Fusion Proteins; D051669:Shab Potassium Channels; D051670:Shaw Potassium Channels; D029867:Xenopus Proteins