Distribution of 5-hydroxytryptamine-immunoreactive boutons on immunohistochemically-identified Renshaw cells in cat and rat lumbar spinal cord.
A combination of anti-gephyrin- and anti-calbindin D28k-immunoreactivity was used to identify 129 and 171 Renshaw cells and their dendrites in cat and rat lumbar spinal cord, respectively. Using anti-5-hydroxytryptamine-immunoreactivity to label serotoninergic fibers and boutons, 1048 serotoninergic boutons were observed in close contact with the immunolabeled Renshaw cells, with an average of 4.4 and 2.8 close contacts on cat and rat Renshaw cells, respectively. Two-thirds of the observed appositions were formed on the somatic membrane.
Carr
P A
PA
Department of Anatomy, Wright State University, P.O. Box 927, Dayton, OH 45435, USA.
Pearson
J C
JC
Fyffe
R E
RE
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D002352:Carrier Proteins / Q000378:metabolism; D002415:Cats; D007150:Immunohistochemistry; D008161:Lumbosacral Region; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D017729:Presynaptic Terminals / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism*; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism*; D014018:Tissue Distribution
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D002352:Carrier Proteins; D008565:Membrane Proteins; D064030:S100 Calcium Binding Protein G; C073441:gephyrin; D012701:Serotonin