Inulase-secreting strain of Saccharomyces cerevisiae produces fructose.
The gene encoding inulase of the yeast Kluyveromyces marxianus (INU1Km) was cloned and expressed in the inulin-negative yeast Saccharomyces cerevisiae. Cells of S. cerevisiae transformed with the INU1Km gene have acquired extracellular inulase activity and were able to grow in the medium with inulin as a sole carbon source. The S. cerevisiae strain was constructed that is capable of heterologous expression of secreted K. marxianus inulase and is defective in fructose uptake due to null-mutations of the hexokinase structural genes HXK1 and HXK2. When grown in inulin-containing media, this strain is capable of accumulating at least 10% glucose-free fructose in the culture liquid.
Brevnova
E E
EE
Institute of Genetics and Selection of Industrial Microorganisms, Moscow 113545, Russia.
Kozlov
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DG
Efremov
B D
BD
Benevolensky
S V
SV
D003001:Cloning, Molecular; D004271:DNA, Fungal / Q000737:chemistry; D005632:Fructose / Q000096:biosynthesis*; D005838:Genotype; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007444:Inulin / Q000378:metabolism; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013696:Temperature
D004271:DNA, Fungal; D005632:Fructose; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase