Distribution of diacylglycerols among plasma lipoproteins in control subjects and in patients with non-insulin-dependent diabetes.
BACKGROUND
Diacylglycerols (DAGs), which are well-known components of insect lipophorins, have been recently recognized as a major glyceride of human high-density lipoprotein (HDL). Moreover, DAGs are good substrates for hepatic lipase and for the phospholipid transfer protein (PLTP). The present work was undertaken to determine the lipoprotein concentrations of DAGs, in control subjects, in non-insulin-dependent diabetic (NIDD) patients and in patients with severe hypertriglyceridaemia.


METHODS
Lipoproteins were isolated from 11 control subjects, 17 diabetic patients and three hypertriglyceridaemic patients, using a combination of ultracentrifugation and precipitation. After lipid extraction, DAGs were separated by thin-layer chromatography and quantified by a glyceride assay.


RESULTS
DAGs were detectable in all lipoprotein fractions of the three groups of subjects. Total DAGs were correlated with total triglycerides (TGs) and even more strikingly with very low-density lipoprotein triglycerides. Although the majority of DAG was recovered in apo B-containing lipoproteins, the proportion of DAG with respect to TG was most elevated in HDL.


CONCLUSIONS
These findings indicate that DAGs are probably formed from TG during lipolysis and that they can be transported to HDL through the action of PLTP. This raises the question whether DAG might act as an inhibitor of phospholipid transfer by competition for binding to PLTP.
Lalanne
F
F
Université Claude Bernard, Lyon, France.
Pruneta
V
V
Bernard
S
S
Ponsin
G
G
D000328:Adult; D001055:Apolipoproteins B / Q000097:blood; D002352:Carrier Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002914:Chylomicrons / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000378:metabolism*; D004075:Diglycerides / Q000097:blood*; D005260:Female; D006801:Humans; D015228:Hypertriglyceridemia / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008875:Middle Aged; D049294:Phospholipid Transfer Proteins; D014280:Triglycerides / Q000097:blood
D001055:Apolipoproteins B; D002352:Carrier Proteins; D002914:Chylomicrons; D004075:Diglycerides; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D008565:Membrane Proteins; D049294:Phospholipid Transfer Proteins; D014280:Triglycerides; D002784:Cholesterol