Myelin-associated oligodendrocytic basic protein is essential for normal arrangement of the radial component in central nervous system myelin.
We previously reported that myelin-associated oligodendrocytic basic protein (MOBP) was abundantly expressed in the central nervous system (CNS) myelin, and shared several characteristics with myelin basic protein (MBP). In particular, a cluster of positively charged amino acids was considered to facilitate compaction of the cytoplasmic face of the myelin sheath, as in the case of MBP. However, the contribution of MOBP in forming and maintaining the myelin sheath still remains unclear. Recent investigations showed that one isoform of MOBP was expressed in the embryo prior to myelination, and MOBP isoforms were colocalized with the microtubular network and nucleus in vitro. To explore the role of MOBP in vivo, we generated MOBP-deficient mice and analysed the CNS myelin. Surprisingly, the compact myelin was formed, however, the myelin from MOBP-deficient mice exposed to hexachlorophene, a known dysmyelinating agent, showed widening of the major dense lines. These results suggest that MOBP is not essential for myelin formation, but reinforces the apposition of the cytoplasmic faces of the myelin sheath. A striking phenotype of MOBP-deficient mice was the presence of the straight 'condensed' radial component. This component has been described as a tight junction-like complex running radially and zig-zag through the CNS myelin sheath between inner and outer mesaxons. These results suggest that MOBP is essential for normal arrangement of the radial component.
Yamamoto
Y
Y
Department of Neurology, Osaka University Medical School, 2-2 Yamada- oka, Suita, Osaka 5650-0871, Japan.
Yoshikawa
H
H
Nagano
S
S
Kondoh
G
G
Sadahiro
S
S
Gotow
T
T
Yanagihara
T
T
Sakoda
S
S
D000818:Animals; D000891:Anti-Infective Agents, Local; D015139:Blotting, Southern; D003593:Cytoplasm / Q000737:chemistry / Q000502:physiology / Q000648:ultrastructure; D005260:Female; D005614:Freeze Fracturing; D006582:Hexachlorophene; D008055:Lipids / Q000032:analysis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008817:Mice, Mutant Strains; D008854:Microscopy, Electron; D009043:Motor Activity / Q000502:physiology; D009185:Myelin Proteins; D009186:Myelin Sheath / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D018992:Myelin-Associated Glycoprotein / Q000235:genetics* / Q000378:metabolism; D063308:Myelin-Oligodendrocyte Glycoprotein; D017830:Octoxynol; D009836:Oligodendroglia / Q000737:chemistry* / Q000502:physiology* / Q000648:ultrastructure; D009900:Optic Nerve / Q000737:chemistry / Q000166:cytology / Q000502:physiology; D010641:Phenotype; D013116:Spinal Cord / Q000737:chemistry / Q000166:cytology / Q000502:physiology
D000891:Anti-Infective Agents, Local; D008055:Lipids; C560488:Mobp protein, mouse; C571327:Mog protein, mouse; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D017830:Octoxynol; D006582:Hexachlorophene