title: Reliable PCR-based method for cloning cDNA in plasmid vectors by frequency estimation.
authors: National Defense Medical College, Saitama, Japan.
authors: K
authors: K
authors: Miura
authors: K
authors: K
authors: Takishima
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002999:Clone Cells; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011336:Probability; D015203:Reproducibility of Results
chemical_list: D018076:DNA, Complementary