Identification, isolation, and characterization of a species-specific 30-kDa antigen of Oesophagostomum dentatum.
In the search for a serology tool for the diagnosis of nonpatent as well as patent infections with Oesophagostomum dentatum in pigs a water-soluble, unglycosilated antigen of about 30 kDa specific for the third-stage larvae of the parasite was purified by ion-exchange chromatography. In Western blots, the antigen was first detected by antibodies at day 7 postinfection. Cross-reactivity with O. quadrispinulatum, Ascaris suum, or Trichuris suis was not detected. It is suggested that this protein is a suitable tool for the species-specific serodiagnosis of O. dentatum infection in pigs.
Joachim
A
A
Institute of Parasitology, Hannover School of Veterinary Medicine, Germany.
Ruttkowski
B
B
Christensen
C M
CM
Daugschies
A
A
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification*; D015153:Blotting, Western; D002852:Chromatography, Ion Exchange; D007525:Isoelectric Focusing; D007814:Larva / Q000276:immunology; D009814:Oesophagostomiasis / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D009815:Oesophagostomum / Q000254:growth & development / Q000276:immunology*; D013045:Species Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology
D000909:Antibodies, Helminth; D000947:Antigens, Helminth