title: Characterization of a cDNA encoding a precursor of Carassius RFamide, structurally related to a mammalian prolactin-releasing peptide.
abstract: We have characterized the cDNA encoding Carassius RFamide (C-RFa), which is structurally related to mammalian prolactin-releasing peptides (PrRPs), from the brain of the crucian carp. The deduced C-RFa precursor has been shown to comprise 117 amino acids, encoding a single C-RFa sequence. A comparative study of amino acid sequences has revealed that several sequences conserved in preproPrRPs are also found in the C-RFa precursor. Furthermore, the abundant presence of the C-RFa mRNA in the telencephalon, optic tectum, medulla oblongata, and proximal half eye ball was demonstrated by Southern blot analysis of RT-PCR products.
authors: Suntory Institute for Bioorganic Research, Mishimagun, Osaka, Japan.
authors: H
authors: H
authors: Satake
authors: H
authors: H
authors: Minakata
authors: X
authors: X
authors: Wang
authors: M
authors: M
authors: Fujimoto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps; D002417:Cattle; D018076:DNA, Complementary; D015870:Gene Expression; D007028:Hypothalamic Hormones / Q000235:genetics*; D008322:Mammals; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D056690:Prolactin-Releasing Hormone; D011498:Protein Precursors / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D007028:Hypothalamic Hormones; D009479:Neuropeptides; D056690:Prolactin-Releasing Hormone; D011498:Protein Precursors; C058047:arginylphenylalaninamide