Genetic optimization of recombinant glycoprotein production by mammalian cells.
Genetically modified mammalian cells are the preferred system for the production of recombinant therapeutic glycoproteins. Other applications include engineering of cell lines for drug screening and cell-based therapies, and the construction of recombinant viruses for gene therapy. This article highlights contemporary core genetic technologies and emerging strategies for genetically engineering mammalian cells for optimal recombinant-protein expression.
Fussenegger
M
M
Institute of Biotechnology, ETH Hönggerberg, Zurich, Switzerland.
Bailey
J E
JE
Hauser
H
H
Mueller
P P
PP
D017209:Apoptosis / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D008967:Molecular Biology / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D011995:Recombination, Genetic; D014158:Transcription, Genetic
D006023:Glycoproteins; D011994:Recombinant Proteins