Late onset administration of oral antioxidants prevents age-related loss of motor co-ordination and brain mitochondrial DNA damage.
We have studied the effect of aging on brain glutathione redox ratio, on brain mitochondrial DNA damage and on motor co-ordination in mice and the possible protective role of late onset administration of sulphur-containing antioxidants. Glutathione redox ratios change to a more oxidized state in whole brain with aging but the changes are much more pronounced when this ratio is measured in brain mitochondria. The levels of 8-oxo-7,8-dihydro-2 '-deoxyguanosine in mitochondrial DNA are much higher in the brain of old animals than in those of young ones. Late onset oral administration of sulphur-containing antioxidants partially prevents oxidation of mitochondrial glutathione and DNA. There is an inverse relationship between age-associated oxidative damage to mitochondrial DNA and motor co-ordination in old mice.
Pallardó
F V
FV
Departamento de Fisiología, Facultad de Medicina, Universitat de Valencia, Spain.
Asensi
M
M
García de la Asunción
J
J
Antón
V
V
Lloret
A
A
Sastre
J
J
Viña
J
J
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000284:Administration, Oral; D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000409:Alanine / Q000494:pharmacology; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects*; D004272:DNA, Mitochondrial / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D004334:Drug Administration Schedule; D005978:Glutathione / Q000378:metabolism / Q000494:pharmacology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology*; D010084:Oxidation-Reduction; D004856:Postural Balance / Q000187:drug effects / Q000502:physiology; D013455:Sulfur / Q000737:chemistry; D013844:Thiazoles / Q000494:pharmacology
C118903:AMTC antioxidant; D000975:Antioxidants; D004272:DNA, Mitochondrial; D013844:Thiazoles; D013455:Sulfur; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine; D005978:Glutathione; D000409:Alanine