title: Chinese hamster ovary cells require the coexpression of microsomal triglyceride transfer protein and cholesterol 7alpha-hydroxylase for the assembly and secretion of apolipoprotein B-containing lipoproteins.
abstract: Due to the absence of microsomal triglyceride transfer protein (MTP), Chinese hamster ovary (CHO) cells lack the ability to translocate apoB into the lumen of the endoplasmic reticulum, causing apoB to be rapidly degraded by an N-acetyl-leucyl-leucyl-norleucinal-inhibitable process. The goal of this study was to examine if expression of MTP, whose genetic deletion is responsible for the human recessive disorder abetalipoproteinemia, would recapitulate the lipoprotein assembly pathway in CHO cells. Unexpectedly, expression of MTP mRNA and protein in CHO cells did not allow apoB-containing lipoproteins to be assembled and secreted by CHO cells expressing apoB53. Although expression of MTP in cells allowed apoB to completely enter the endoplasmic reticulum, it was degraded by a proteolytic process that was inhibited by dithiothreitol (1 mM) and chloroquine (100 microM), but resistant to N-acetyl-leucyl-leucyl-norleucinal. In marked contrast, coexpression of the liver-specific gene product cholesterol 7alpha-hydroxylase with MTP resulted in levels of MTP lipid transfer activity that were similar to those in mouse liver and allowed intact apoB53 to be secreted as a lipoprotein particle. These data suggest that, although MTP-facilitated lipid transport is not required for apoB translocation, it is required for the secretion of apoB-containing lipoproteins. We propose that, in CHO cells, MTP plays two roles in the assembly and secretion of apoB-containing lipoproteins: 1) it acts as a chaperone that facilitates apoB53 translocation, and 2) its lipid transfer activity allows apoB-containing lipoproteins to be assembled and secreted. Our results suggest that the phenotype of the cell (e.g. expression of cholesterol 7alpha-hydroxylase by the liver) may profoundly influence the metabolic relationships determining how apoB is processed into lipoproteins and/or degraded.
authors: Mammalian Cell and Molecular Biology Laboratory, Department of Biology, and the Molecular Biology Institute, San Diego State University, San Diego, California 92182-4614, USA.
authors: J F
authors: JF
authors: Fleming
authors: G M
authors: GM
authors: Spitsen
authors: T Y
authors: TY
authors: Hui
authors: L
authors: L
authors: Olivier
authors: E Z
authors: EZ
authors: Du
authors: M
authors: M
authors: Raabe
authors: R A
authors: RA
authors: Davis
mesh_terms: D000818:Animals; D001055:Apolipoproteins B / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D016466:CHO Cells; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000378:metabolism; D002738:Chloroquine / Q000494:pharmacology; D002790:Cholesterol 7-alpha-Hydroxylase / Q000096:biosynthesis* / Q000378:metabolism; D006224:Cricetinae; D004229:Dithiothreitol / Q000494:pharmacology; D004721:Endoplasmic Reticulum; D006367:HeLa Cells; D006801:Humans; D007976:Leupeptins / Q000494:pharmacology; D008074:Lipoproteins / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology; D051379:Mice; D014162:Transfection
chemical_list: D001055:Apolipoproteins B; D002352:Carrier Proteins; D007976:Leupeptins; D008074:Lipoproteins; C088653:apolipoprotein B-53; C106092:microsomal triglyceride transfer protein; C063261:acetylleucyl-leucyl-norleucinal; D002738:Chloroquine; D002790:Cholesterol 7-alpha-Hydroxylase; D004229:Dithiothreitol