Development of an automated microbial sensor system.
An automated whole cell biosensor system was developed by integration of immobilized microbial cells in a flow-through system with screen-printed flow-through electrodes as detectors. The detectors used were thick-film Pt-electrodes in a 3-electrode configuration constructed as sandwich flow-through cells with a volume of about 36 microliters polarized at -900 mV. The measuring principle was the determination of oxygen consumption due to the microbial metabolism. Fructose was used as model analyte. The microorganisms were immobilized on cellulose-acetate membranes and integrated into a newly created reaction chamber (membrane reactor). The microbial cells used were Rhodococcus erythropolis and Issatchenkia orientalis known to be suitable for the determination of biological oxygen demand.
Heim
S
S
Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Schnieder
I
I
Binz
D
D
Vogel
A
A
Bilitewski
U
U
D015374:Biosensing Techniques; D017022:Flow Injection Analysis; D010101:Oxygen Consumption; D012240:Rhodococcus / Q000378:metabolism*