Enzyme array-amperometric detection in carbohydrate analysis.
The introduction of an enzyme array-electrochemical detection method for carbohydrate analysis is demonstrated by using two complex and one high mannose N-linked oligosaccharides. Instead of measuring the remaining uncleaved oligosaccharides in enzymatic digestion, released monosaccharides are directly quantified by pulsed amperometric detection at a gold electrode. The measured monosaccharide concentrations in combination with the enzyme array analysis provide structural characterization of oligosaccharides. The enzyme array-electrochemical detection method does not require any separation procedure or any prior labeling of oligosaccharides. However, this method is limited by the use of purified oligosaccharide samples and the nature of the enzyme array. The development of more sophisticated enzyme arrays relies upon the introduction of a bank of highly specific (bond, arm, aglycon) exoglycosidases.
Sun
M
M
Department of Chemistry and Ames Laboratory, USDOE, Iowa State University, Ames, Iowa 50011, USA.
Lee
C S
CS
D050260:Carbohydrate Metabolism; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004563:Electrochemistry / Q000379:methods*; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D012680:Sensitivity and Specificity
D002241:Carbohydrates; D009844:Oligosaccharides; D006026:Glycoside Hydrolases