title: Phagosomes are fully competent antigen-processing organelles that mediate the formation of peptide:class II MHC complexes.
abstract: During the processing of particulate Ags, it is unclear whether peptide:class II MHC (MHC-II) complexes are formed within phagosomes or within endocytic compartments that receive Ag fragments from phagosomes. Murine macrophages were pulsed with latex beads conjugated with OVA. Flow or Western blot analysis of isolated phagosomes showed extensive acquisition of MHC-II, H-2M, and invariant chain within 30 min, with concurrent degradation of OVA. T hybridoma responses to isolated subcellular fractions demonstrated OVA (323-339):I-Ad complexes in phagosomes and plasma membrane but not within dense late endocytic compartments. Furthermore, when two physically separable sets of phagosomes were present within the same cells, OVA(323-339):I-Ad complexes were demonstrated in latex-OVA phagosomes but not in phagosomes containing latex beads conjugated with another protein. This implies that these complexes were formed specifically within phagosomes and were not formed elsewhere and subsequently transported to phagosomes. In addition, peptide:MHC-II complexes were shown to traffic from phagosomes to the cell surface. In conclusion, phagosomes are fully competent to process Ags and generate peptide:MHC-II complexes that are transported to the cell surface and presented to T cells.
authors: Institute of Pathology, Case Western Reserve University, Cleveland, OH 44106, USA.
authors: L
authors: L
authors: Ramachandra
authors: R
authors: R
authors: Song
authors: C V
authors: CV
authors: Harding
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000096:biosynthesis; D000944:Antigens, Differentiation, B-Lymphocyte / Q000032:analysis; D001692:Biological Transport / Q000276:immunology; D015153:Blotting, Western; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D005434:Flow Cytometry; D006681:HLA-D Antigens / Q000032:analysis; D000949:Histocompatibility Antigens Class II / Q000032:analysis / Q000096:biosynthesis / Q000378:metabolism*; D051907:Lysosomal Membrane Proteins; D046911:Macromolecular Substances; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008863:Microspheres; D009113:Muramidase / Q000032:analysis; D010047:Ovalbumin / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D010455:Peptides / Q000276:immunology / Q000378:metabolism*; D010588:Phagosomes / Q000201:enzymology / Q000276:immunology* / Q000378:metabolism*; D013347:Subcellular Fractions / Q000737:chemistry; D013601:T-Lymphocytes / Q000737:chemistry
chemical_list: D015703:Antigens, CD; D000944:Antigens, Differentiation, B-Lymphocyte; C416189:H2-M antigens; D006681:HLA-D Antigens; C084245:HLA-DM antigens; D000949:Histocompatibility Antigens Class II; D051907:Lysosomal Membrane Proteins; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; C111390:OVA 323-339; D010446:Peptide Fragments; D010455:Peptides; C045084:invariant chain; D010047:Ovalbumin; D009113:Muramidase