Molecular cloning and analysis of Ca2+/calmodulin-dependent protein kinase II from the chicken brain.
The goals of this study were to identify specific mRNA for isoforms of calmodulin-dependent protein kinase II in chicken forebrain, prepare a cDNA expression library, and perform a sequence analysis of the kinase cDNA. Specific mRNAs for alpha- and beta-subunits of the kinase were identified in Northern blots. The mRNA for the alpha-subunit is larger in the chicken that in the rat, and for the beta-subunit is smaller in the chicken than the rat. Nucleotide sequencing of selected clones demonstrated the presence of an alpha-subunit with a 33 nucleotide insert known as the alpha-B-isoform. Clones of the beta-subunit showed it to contain a deletion of six nucleotides relative to previously described sequences. Variability in the mRNAs of calmodulin kinase II, as shown here, reflect the presence of species-dependent variability in gene structure as well as the presence of different functional isoforms.
Li
G
G
Department of Anatomical Sciences, University of Louisville, KY, USA.
Liu
N
N
Liu
O L
OL
Mower
G D
GD
Gregg
R G
RG
Cooper
N G
NG
D000818:Animals; D015152:Blotting, Northern; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry / Q000235:genetics*; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000235:genetics*; D002645:Chickens; D003001:Cloning, Molecular; D015971:Gene Expression Regulation, Enzymologic; D007527:Isoenzymes / Q000235:genetics*; D008969:Molecular Sequence Data; D051381:Rats; D017386:Sequence Homology, Amino Acid
D007527:Isoenzymes; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2; D017871:Calcium-Calmodulin-Dependent Protein Kinases