[Effect of lysophasphatidylcholine on sensitivity of the heart to acetylcholine and binding of quinuclidinyl benzilate to myocardial membranes].
Chemical nature of the blood serum factor exerting cholinolytic effect on the animal heart, was determined. The effect is due to the serum lipid component: lysophosphatidylcholine. The latter in concentration 1-25 microM suppresses the sensitivity of the frog perfused heart ventricle to acetylcholine. Phosphatidylcholine was found to protect the heart from the lysophosphatidylcholine effect. Lysophosphatidylcholine also affected the binding of the acetylcholine high affinity antagonist [3H]-quinuclidinylbenzilate to the rabbit atria cell membranes. The mechanism of this effect is related to an increased ability of muscarinic receptors to form oligomeric complexes in presence of lysophosphatidylcholine, the latter manifesting a positive cooperativity on binding to ligand.
Prokazova
N V
NV
Cardiology Research Centre, Moscow, Russia.
Zvezdina
N D
ND
Suslova
I V
IV
Korotaeva
A A
AA
Turpaev
T M
TM
D000109:Acetylcholine / Q000037:antagonists & inhibitors*; D000818:Animals; D001769:Blood; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines / Q000378:metabolism* / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D011813:Quinuclidinyl Benzilate / Q000378:metabolism*; D011817:Rabbits; D011896:Rana temporaria; D011976:Receptors, Muscarinic / Q000378:metabolism
D008244:Lysophosphatidylcholines; D018727:Muscarinic Antagonists; D011976:Receptors, Muscarinic; D011813:Quinuclidinyl Benzilate; D000109:Acetylcholine