Molecular characterization of two light-induced, gamete-specific genes from Chlamydomonas reinhardtii that encode hydroxyproline-rich proteins.
Gametic differentiation in Chlamydomonas reinhardtii is a two-step process, which is controlled by the sequential action of the two extrinsic signals, nitrogen starvation and blue light. The gamete-specific genes GAS28 and GAS29 are expressed in the late phase of gametogenesis. Their light-induced expression is restricted to cells that have completed the first, nitrogen starvation-activated, phase of differentiation. A comparison of the two genes revealed striking similarities as well as differences. Their most prominent shared feature is an extended sequence homology of over 90% in their 5'-untranslated regions, suggesting a role in translational regulation. GAS28 and GAS29 both encode hydroxyproline-rich proteins (HRGPs) of very similar sizes that exhibit typical features of volvocalean cell wall constituents. GAS28 shows a high degree of homology with the Volvox pherophorin gene family, suggesting a relationship between these genes.
Rodriguez
H
H
Institut für Biologie III, Universität Freiburg, Germany.
Haring
M A
MA
Beck
C F
CF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016825:Chlamydomonas reinhardtii / Q000235:genetics*; D003001:Cloning, Molecular; D016054:DNA, Protozoan; D015870:Gene Expression; D017125:Genes, Protozoan; D005854:Germ Cells; D006023:Glycoproteins / Q000235:genetics*; D006909:Hydroxyproline; D008027:Light; D008969:Molecular Sequence Data; D016296:Mutagenesis; D015800:Protozoan Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D016054:DNA, Protozoan; C118426:GAS28 protein, Chlamydomonas reinhardtii; C118427:GAS29 protein, Chlamydomonas reinhardtii; D006023:Glycoproteins; D015800:Protozoan Proteins; D006909:Hydroxyproline