Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras.
Monitoring the migrations of cells during embryonic development requires a system in which cells can be identified in situ during locomotion. One promising system involves the generation of chimeras by transplanting mouse cells into chick embryos in ovo to exploit the wealth of mouse genetic variants. The success of this technique relies on the ability to detect individual mouse cells in a chick environment with high specificity. The murine B2 family of short interspersed elements is present in the mouse genome at copy numbers in excess of 10(5), whereas this sequence is absent in the chick genome based on hybridization techniques. This differential of five orders of magnitude produces signals in mouse cells that are easily identified, even in an environment that is predominantly chick. Thus, the B2 repeat probe is highly effective for the purpose of identifying mouse cells in mouse-chick chimeras.
Bollag
R J
RJ
Developmental Biology Program, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, 30912, USA. rbollag@mail.mcg.edu
Crawford
K B
KB
Stadt
H
H
Kumiski
D
D
Zdanowicz
M
M
Baptista
C
C
Herlea
V
V
Kirby
M L
ML
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D017690:Cell Transplantation; D002642:Chick Embryo; D051379:Mice; D012091:Repetitive Sequences, Nucleic Acid