Phospholipase A2-like catalytic antibody.
The phospholipase A2-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a kcat of 1.0 x 10(-2) min(-1) and aKm of 71 microM. This antibody hydrolyzes the C2 ester in (R)-2, regio- and enantioselectively.
Isomura
S
S
Faculty of Pharmacy, Meijo University, Yagotoyama, Tempaku, Nagoya, Japan.
Ito
K
K
Haruna
M
M
D017151:Antibodies, Catalytic / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D010743:Phospholipids / Q000378:metabolism; D016339:Spectrometry, Mass, Fast Atom Bombardment; D013379:Substrate Specificity
D017151:Antibodies, Catalytic; D010743:Phospholipids; D010741:Phospholipases A; D054467:Phospholipases A2