[The control survey in CD marker analysis of leukemic cells].
Analysis of cell surface antigens is thought to be more objective compared to classification by the morphology. However, it has been indicated that the evaluated data are different each other between institutes. Therefore, we performed a control survey of CD marker analysis in leukemia cells using flow cytometry in six commercial laboratories. The expression of CD2, 4, 5, 7, 8, 10, 13, 14, 19, 20, 33, 34, 38, and 71, HLA-DR in tumor cells from megakaryoblastic leukemia, lymphoblastic crisis of CML, and ALL patients, were examined. There were large differences in the results of CD marker analysis among the six commercial laboratories. One possible reason for this result is the differences in gating condition and monoclonal antibodies used in each laboratory. However, there were the differences in the results even when the same gating condition and antibodies were used. The reasons for the difference in transport or treatment conditions for samples remain a matter of considerable controversy. In the present condition, it was suggested that the exchange of information about the origin of tumor cells between the physician in charge and examination experts is thought to be the best way to analyze proper cell surface antigen expression. Further, no national survey has been carried out, but the establishment of a standard assay is preferred to obtain proper data for the patients and medical parties concerned.
Sato
K
K
Dept. of Laboratory Diagnosis, Sapporo Medical University, School of Medicine.
Kimura
T
T
Araake
H
H
Kida
T
T
Kobayashi
D
D
Yagihashi
A
A
Kudoh
T
T
Sakamaki
S
S
Watanabe
N
N
D000293:Adolescent; D000368:Aged; D000911:Antibodies, Monoclonal; D015703:Antigens, CD / Q000032:analysis*; D000954:Antigens, Surface / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000145:classification / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D007947:Leukemia, Megakaryoblastic, Acute / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology; D008297:Male; D008875:Middle Aged
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D000954:Antigens, Surface