title: The choline-converting pathway in Staphylococcus xylosus C2A: genetic and physiological characterization.
abstract: A Staphylococcus xylosus C2A gene cluster, which encodes enzymes in the pathway for choline uptake and dehydrogenation (cud), to form the osmoprotectant glycine betaine, was identified. The cud locus comprises four genes, three of which encode proteins with significant similarities to those known to be involved in choline transport and conversion in other organisms. The physiological role of the gene products was confirmed by analysis of cud deletion mutants. The fourth gene possibly codes for a regulator protein. Part of the gene cluster was shown to be transcriptionally regulated by choline and elevated NaCl concentrations as inducers.
authors: Mikrobielle Genetik, Universität Tübingen, 72076 Tübingen, Germany. ralf.rosenstein@uni-tuebingen.de
authors: R
authors: R
authors: Rosenstein
authors: D
authors: D
authors: Futter-Bryniok
authors: F
authors: F
authors: Götz
mesh_terms: D000429:Alcohol Oxidoreductases; D000445:Aldehyde Oxidoreductases; D001426:Bacterial Proteins / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D001622:Betaine / Q000378:metabolism; D050728:Betaine-Aldehyde Dehydrogenase; D002352:Carrier Proteins / Q000235:genetics* / Q000502:physiology; D002384:Catalysis; D002794:Choline / Q000378:metabolism*; D050545:Choline Dehydrogenase; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D016208:Databases, Factual; D005798:Genes, Bacterial; D026901:Membrane Transport Proteins; D008969:Molecular Sequence Data; D005810:Multigene Family; D016296:Mutagenesis; D013210:Staphylococcus / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D026901:Membrane Transport Proteins; C065637:choline transporter; D001622:Betaine; D000429:Alcohol Oxidoreductases; D050545:Choline Dehydrogenase; D000445:Aldehyde Oxidoreductases; D050728:Betaine-Aldehyde Dehydrogenase; C118381:CUDA protein, Staphylococcus xylosus; D002794:Choline