title: Inhibition of human cytomegalovirus protease by enedione derivatives of thieno[2,3-d]oxazinones through a novel dual acylation/alkylation mechanism.
abstract: Enedione derivatives of thieno[2,3-d]oxazinones are nanomolar inhibitors of CMV protease which act through a novel dual acylation of the catalytic serine and alkylation of the protease cysteine 161 via a Michael addition to the enedione moiety of the inhibitor.
authors: SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UK. Ivan_Pinto-1@sbphrd.com
authors: I L
authors: IL
authors: Pinto
authors: R L
authors: RL
authors: Jarvest
authors: B
authors: B
authors: Clarke
authors: C E
authors: CE
authors: Dabrowski
authors: A
authors: A
authors: Fenwick
authors: M M
authors: MM
authors: Gorczyca
authors: L J
authors: LJ
authors: Jennings
authors: P
authors: P
authors: Lavery
authors: E J
authors: EJ
authors: Sternberg
authors: D G
authors: DG
authors: Tew
authors: A
authors: A
authors: West
mesh_terms: D000215:Acylation; D000478:Alkylation; D002470:Cell Survival / Q000187:drug effects; D008958:Models, Molecular; D010078:Oxazines / Q000737:chemistry / Q000494:pharmacology*; D012697:Serine Endopeptidases / Q000187:drug effects*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D016339:Spectrometry, Mass, Fast Atom Bombardment; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010078:Oxazines; D015842:Serine Proteinase Inhibitors; D012697:Serine Endopeptidases; C071910:assemblin