Beauveriolides, specific inhibitors of lipid droplet formation in mouse macrophages, produced by Beauveria sp. FO-6979.
Beauveria sp. FO-6979, a soil isolate, was found to produce inhibitors of lipid droplet formation in mouse peritoneal macrophages. A new compound beauveriolide III was isolated along with a known compound beauveriolide I from the fermentation broth of the producing strain by solvent extraction, ODS column chromatography, silica gel column chromatography and preparative HPLC. Beauveriolides I and III caused a reduction in the number and size of cytosolic lipid droplets in macrophages at 10 microM without any cytotoxic effect on macrophages.
Namatame
I
I
Graduate School of Pharmaceutical Sciences, Kitasato University, and Research Center for Biological Function, The Kitasato Institute, Tokyo, Japan.
Tomoda
H
H
Si
S
S
Yamaguchi
Y
Y
Masuma
R
R
Omura
S
S
D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D000969:Antinematodal Agents / Q000494:pharmacology; D001203:Ascomycota / Q000145:classification / Q000378:metabolism* / Q000648:ultrastructure; D001419:Bacteria / Q000187:drug effects; D047630:Depsipeptides; D005260:Female; D005285:Fermentation; D066298:In Vitro Techniques; D007306:Insecticides / Q000494:pharmacology; D050356:Lipid Metabolism; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010456:Peptides, Cyclic / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D000969:Antinematodal Agents; D047630:Depsipeptides; D007306:Insecticides; D010456:Peptides, Cyclic; C013833:beauverolides