title: Relation of plasma leptin to lipoproteins in overweight children undergoing weight reduction.
abstract: BACKGROUND
  In obese children, plasma leptin is elevated and correlates with the body mass index (BMI). In obese adults, plasma leptin decreases during weight reduction. Since the leptin system changes dynamically in puberty, we asked whether weight reduction in obese adolescents has similar consequences for plasma leptin as in overweight adults. In plasma, a portion of leptin is bound to several as yet uncharacterised proteins. We therefore studied the possible association of leptin with plasma lipoproteins.
  
  
  METHODS
  We measured plasma leptin, lipoprotein cholesterol and apolipoproteins (apo) A-I and B in 34 obese children (age 12.5+/-1.9 y, relative BMI 165.0+/-28.1%) before and after three weeks of weight reduction in a dietary camp. Lipoprotein binding of endogenous and exogenously radiolabelled leptin was studied by preparative ultracentrifugation.
  
  
  RESULTS
  Plasma leptin was higher in obese children than in normal weight controls and fell from 16.5+/-9.8 ng/ml to 10.0+/-8.6 ng/ml after weight reduction (P < 0.001). In multivariate regression, relative BMI and apoA-I were significant predictors of baseline leptin and accounted for 38% (P = 0.003) and 15% (P = 0.006) of the variance of baseline leptin concentrations in obese children. Only the difference in plasma high-density lipoprotein (HDL)-cholesterol independently predicted the change of plasma leptin that was associated with weight reduction, explaining 29% of the variance of leptin changes (P = 0.0032). A substantial portion of both endogenous and exogenously labelled leptin was recovered with HDL isolated by ultracentrifugation.
  
  
  CONCLUSIONS
  We conclude that plasma leptin decreases in overweight children undergoing short term weight reduction. In obese children, plasma apoA-I and HDL-cholesterol are independent predictors of leptin concentrations during weight loss, respectively. In addition, HDLs transport a variable portion of leptin in the circulation.
authors: Department of Medical Chemistry, University of Vienna, Austria.
authors: M
authors: M
authors: Holub
authors: K
authors: K
authors: Zwiauer
authors: C
authors: C
authors: Winkler
authors: B
authors: B
authors: Dillinger-Paller
authors: E
authors: E
authors: Schuller
authors: E
authors: E
authors: Schober
authors: S
authors: S
authors: Stöckler-Ipsiroglou
authors: W
authors: W
authors: Patsch
authors: W
authors: W
authors: Strobl
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood; D001823:Body Composition; D015992:Body Mass Index; D002648:Child; D008076:Cholesterol, HDL / Q000097:blood; D004038:Diet, Reducing; D005260:Female; D006801:Humans; D020738:Leptin; D017270:Lipoprotein(a) / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood* / Q000178:diet therapy; D011506:Proteins / Q000378:metabolism*; D015431:Weight Loss
chemical_list: D016632:Apolipoprotein A-I; D008076:Cholesterol, HDL; D020738:Leptin; D017270:Lipoprotein(a); D008074:Lipoproteins; D008075:Lipoproteins, HDL; D011506:Proteins