Quantitative analysis of the kinetics of phospholipase A2 using fast atom bombardment mass spectrometry.
Fast atom bombardment mass spectrometry that can directly analyze lysophospholipids was used to quantitatively determine the kinetics of phospholipase A2. This method is 1250 times more sensitive than the colorimetric assay.
Isomura
S
S
Faculty of Pharmacy, Meijo University, Tempaku, Nagoya, Japan.
Ito
K
K
Haruna
M
M
D007700:Kinetics; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism; D015394:Molecular Structure; D010741:Phospholipases A / Q000378:metabolism*; D054467:Phospholipases A2; D012680:Sensitivity and Specificity; D016339:Spectrometry, Mass, Fast Atom Bombardment
D008246:Lysophospholipids; D010741:Phospholipases A; D054467:Phospholipases A2