Immunomodulatory principles of Dichrocephala bicolor.
From the ethanolic extract of Dichrocephala bicolor eight compounds-4,5-dicaffeoyl quinic acid (1); 3,4-dicaffeoyl quinic acid (2); 3,5-dicaffeoyl quinic acid (3); ethyl 4,5-dicaffeoyl quinate (4); methyl 3,5-dicaffeoyl quinate (5); 5-caffeoyl quinic acid (6); caffeic acid (7); and quercetin-3-O-rutinoside (8)-were isolated and identified. All of them were selected for immunopharmacological activity testing. Human mononuclear cells (HMNC) were used as target cells. Cell proliferation was determined by 3H-thymidine uptake. Compounds 2 and 6 potently enhanced HMNC proliferation and interferon-gamma production. Enhancement mechanisms may involve the increase of cytokines production.
Lin
L C
LC
National Research Institute of Chinese Medicine, Shih-Pai, Taipei 11221, Taiwan, Republic of China.
Kuo
Y C
YC
Chou
C J
CJ
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000096:biosynthesis; D008214:Lymphocytes / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology*; D016339:Spectrometry, Mass, Fast Atom Bombardment
D000276:Adjuvants, Immunologic; D010936:Plant Extracts; D011801:Quinic Acid; D007371:Interferon-gamma