title: Chromatin remodeling and activation of chromosomal DNA replication by an acidic transcriptional activation domain from BRCA1.
abstract: An increasing number of transcription factors have been shown to activate DNA replication. However, the underlying mechanism remains to be elucidated. Here it is shown that when tethered to a cellular replication origin, the acidic transcriptional activation domain of the breast cancer protein BRCA1 alters the local chromatin structure and stimulates chromosomal DNA replication. Cancer-predisposing mutations in BRCA1 that abolish transcriptional activation also prevent chromatin remodeling and activation of replication. Chromatin remodeling occurs even in the absence of a functional replication origin. Thus, increasing chromatin accessibility may be an important mechanism used by transcription factors to facilitate multiple nuclear processes.
authors: Department of Biochemistry and Molecular Genetics, Health Sciences Center, University of Virginia, Charlottesville, Virginia 22908, USA.
authors: Y F
authors: YF
authors: Hu
authors: Z L
authors: ZL
authors: Hao
authors: R
authors: R
authors: Li
mesh_terms: D019313:BRCA1 Protein / Q000235:genetics*; D002843:Chromatin / Q000235:genetics*; D015825:Chromosomes, Fungal; D004261:DNA Replication / Q000235:genetics*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D015533:Transcriptional Activation
chemical_list: D019313:BRCA1 Protein; D002843:Chromatin