Enumeration of antigen-presenting cells in mice infected with Sendai virus.
Substantial progress has been made in understanding Ag presentation to T cells; however, relatively little is known about the location and frequency of cells presenting viral Ags during a viral infection. Here, we took advantage of a highly sensitive system using lacZ-inducible T cell hybridomas to enumerate APCs during the course of respiratory Sendai virus infection in mice. Using lacZ-inducible T cell hybridomas specific for the immunodominant hemagglutinin-neuraminidase HN421-436/I-Ab and nucleoprotein NP324-332/Kb epitopes, we detected APCs in draining mediastinal lymph nodes (MLNs), in cervical lymph nodes, and also in the spleen. HN421-436/I-Ab- and NP324-332/Kb-presenting cells were readily detectable between days 3 and 9 postinfection, with more APCs present in the MLN than in the cervical lymph nodes. Interestingly, no infectious virus was detected in lymphoid tissue beyond day 6, suggesting that a depot of noninfectious viral Ag survives, in some form, for 2-3 days after viral clearance. Fractionation of the MLN demonstrated that APC frequency was enriched in dendritic cells and macrophages but depleted in the B cell population, suggesting that B cells do not form a large population of APCs during the primary response to this virus.
Usherwood
E J
EJ
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
Hogg
T L
TL
Woodland
D L
DL
D000595:Amino Acid Sequence; D000818:Animals; D000938:Antigen-Presenting Cells / Q000166:cytology* / Q000276:immunology / Q000821:virology; D000956:Antigens, Viral / Q000378:metabolism; D002452:Cell Count / Q000379:methods*; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism; D005260:Female; D006825:Hybridomas / Q000166:cytology / Q000821:virology; D016130:Immunophenotyping; D007763:Lac Operon / Q000276:immunology; D008213:Lymphocyte Activation / Q000235:genetics; D008221:Lymphoid Tissue / Q000166:cytology; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D018108:Respirovirus / Q000276:immunology*; D010253:Respirovirus Infections / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology
D000956:Antigens, Viral; D018984:Epitopes, T-Lymphocyte