Reliable PCR-based method for cloning cDNA in plasmid vectors by frequency estimation.
Miura
K
K
National Defense Medical College, Saitama, Japan.
Takishima
K
K
D000818:Animals; D001921:Brain / Q000378:metabolism; D002417:Cattle; D002999:Clone Cells; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D015723:Gene Library; D006801:Humans; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011336:Probability; D015203:Reproducibility of Results
D018076:DNA, Complementary