title: Binding and catalytic properties of the Cdc2 and Crp proteins of Dictyostelium.
abstract: Dictyostelium expresses at least two proteins of the cyclin-dependent kinase (Cdk) family, Cdc2 and Crp. Cdc2 levels remain relatively constant during differentiation, whereas the levels of Crp increase dramatically as differentiation progresses. Crp is highly related to the mammalian Cdk5, and p25 (a truncated form of p35, the activating subunit of Cdk5 from mammalian brain) stimulates the histone H1 kinase activity of GST-Crp by several fold. In contrast, p25 does not stimulate the histone H1 kinase activity of GST-Cdc2 or the Cdc2 activity present in cell extracts from vegetative Dictyostelium cells. GST-Cdc2, in vitro translated Cdc2 and Cdc2 from all stages of differentiation bind to p13suc1. In contrast, GST-Crp, in vitro translated Crp and the Crp protein present in cell extracts do not bind to p13suc1. We have confirmed a previous report by Arakane and Maeda [J. Plant Res. (1997) 110, 81-85] that there is a peak of p13suc1 bound histone H1 kinase activity during late development, but we found that there was no corresponding peak of p13suc1 bound Cdc2 protein that corresponds to this activity. Taken together, these data suggest that neither Cdc2 nor Crp is responsible for the late developmental peak of histone H1 kinase activity that binds to p13suc1.
authors: Department of Microbiology, University of British Columbia, Vancouver, Canada.
authors: S K
authors: SK
authors: Sharma
authors: C
authors: C
authors: Michaelis
authors: K Y
authors: KY
authors: Lee
authors: J H
authors: JH
authors: Wang
authors: G
authors: G
authors: Weeks
mesh_terms: D000818:Animals; D001665:Binding Sites; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D002384:Catalysis; D018797:Cell Cycle Proteins; D004023:Dictyostelium / Q000201:enzymology* / Q000378:metabolism; D005656:Fungal Proteins / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D015800:Protozoan Proteins / Q000378:metabolism; D029702:Schizosaccharomyces pombe Proteins; D012685:Sepharose / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D005656:Fungal Proteins; D015800:Protozoan Proteins; D029702:Schizosaccharomyces pombe Proteins; C051655:Suc1 protein, S pombe; D012685:Sepharose; C084139:Crp protein, Dictyostelium; D017346:Protein Serine-Threonine Kinases; D016203:CDC2 Protein Kinase