Hormonal control of rat fetal pancreas development.
Rat fetal pancreas development and maturation were investigated in vitro and in vivo, and the informations available on their controls do not agree. Our main objective was to reinvestigate fetal pancreas growth in vivo through treatments of the dams during their entire pregnancy. Pregnant rats were thus implanted subcutaneously with Alzet minipumps and received cerulein (0.25 microg kg-1 h-1), gastrin-releasing peptide (GRP; 0.18 microg kg-1 h-1), GRP antagonist (12 microg kg-1 h-1), pentagastrin (2.38 microg kg-1 h-1), L-365,260, a cholecystokinin B (CCKB) receptor antagonist (120 microg kg-1 h-1), and hydrocortisone (417 or 833 microg kg-1 h-1). After sacrifice at the end of pregnancy, the pancreata of the dams and those of their fetuses were excised for weight, protein, RNA, DNA, and digestive enzyme determinations. In the fetus, pancreas growth defined as hyperplasia was observed only in response to hydrocortisone, while aplasia occurred in response to cerulein. Gastrin and the GRP antagonist were the most effective hypertrophic agents, and the effect of the CCKB receptor antagonist was atrophic. In conclusion, hydrocortisone caused proliferation of the fetal rat pancreas, whereas gastrin induced its differentiation and maturation probably through CCKB receptor occupation.
Morisset
J
J
Service de Gastroentérologie, Faculté de Médecine, Université de Sherbrooke, Qué., Canada. jmori7@courrier.usherb.ca
Lainé
J
J
Mimeau-Worthington
T
T
D000818:Animals; D001570:Benzodiazepinones / Q000494:pharmacology; D002108:Ceruletide / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005260:Female; D019886:Gastrin-Releasing Peptide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D008431:Maternal-Fetal Exchange; D009929:Organ Size / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000196:embryology* / Q000201:enzymology; D010418:Pentagastrin / Q000494:pharmacology; D010671:Phenylurea Compounds / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin / Q000037:antagonists & inhibitors
D001570:Benzodiazepinones; D006728:Hormones; D010671:Phenylurea Compounds; D043887:Receptor, Cholecystokinin B; D011949:Receptors, Cholecystokinin; C058121:L 365260; D019886:Gastrin-Releasing Peptide; D002108:Ceruletide; D004247:DNA; D010418:Pentagastrin; D006854:Hydrocortisone