Quantitative determination of E5880 in rat plasma by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry.
A simple and sensitive method is described for the determination of E5880 in rat plasma. The method is based on high-performance liquid chromatography/electrospray ionization mass spectrometry, using deuterated E5880 as an internal standard. Selected reaction monitoring is employed for selectivity and sensitivity, this in turn enables quantification in a short period of time (within 7 minutes) over the extended range of 0.1-1000 ng/ml with acceptable precision and accuracy. The method demonstrated to be suitable for the quantitative analysis of E5880 in rat plasma. The pharmacokinetic profile of E5880 after a single intravenous administration of E5880 was elucidated.
Kikuchi
K
K
Tsukuba Research Laboratories, Eisai Co. Ltd, Ibaraki, Japan.
Sano
Y
Y
Taniguchi
S
S
Matsui
K
K
Namiki
M
M
Ito
H
H
Sakurai
H
H
Yoshimura
T
T
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003903:Deuterium / Q000032:analysis; D007275:Injections, Intravenous; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D015394:Molecular Structure; D010880:Piperidines / Q000097:blood* / Q000493:pharmacokinetics; D011726:Pyridinium Compounds / Q000097:blood* / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D010880:Piperidines; D011726:Pyridinium Compounds; C072429:E 5880; D003903:Deuterium