Genetic evidence for the role of GDP-mannose in plant ascorbic acid (vitamin C) biosynthesis.
Vitamin C (L-ascorbic acid; AsA) acts as a potent antioxidant and cellular reductant in plants and animals. AsA has long been known to have many critical physiological roles in plants, yet its biosynthesis is only currently being defined. A pathway for AsA biosynthesis that features GDP-mannose and L-galactose has recently been proposed for plants. We have isolated a collection of AsA-deficient mutants of Arabidopsis thaliana that are valuable tools for testing of an AsA biosynthetic pathway. The best-characterized of these mutants (vtc1) contains approximately 25% of wild-type AsA and is defective in AsA biosynthesis. By using a combination of biochemical, molecular, and genetic techniques, we have demonstrated that the VTC1 locus encodes a GDP-mannose pyrophosphorylase (mannose-1-P guanyltransferase). This enzyme provides GDP-mannose, which is used for cell wall carbohydrate biosynthesis and protein glycosylation as well as for AsA biosynthesis. In addition to genetically defining the first locus involved in AsA biosynthesis, this work highlights the power of using traditional mutagenesis techniques coupled with the Arabidopsis Genome Initiative to rapidly clone physiologically important genes.
Conklin
P L
PL
Boyce Thompson Institute for Plant Research and Section of Genetics and Development, Cornell University, Ithaca, NY 14853-1801, USA. PLjC3@cornell.edu
Norris
S R
SR
Wheeler
G L
GL
Williams
E H
EH
Smirnoff
N
N
Last
R L
RL
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D001205:Ascorbic Acid / Q000096:biosynthesis*; D001483:Base Sequence; D002250:Carbon Radioisotopes; D018076:DNA, Complementary; D005816:Genetic Complementation Test; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009153:Mutagens / Q000494:pharmacology; D009713:Nucleotidyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011865:Radioisotope Dilution Technique; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D002250:Carbon Radioisotopes; D018076:DNA, Complementary; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D006155:Guanosine Diphosphate Mannose; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase; D008358:Mannose; D001205:Ascorbic Acid