Dot-enzyme linked immunosorbent assay for demonstration of Newcastle disease virus infection.
Dot-enzyme linked immunosorbent assay (ELISA) was standardised to detect Newcastle disease virus (NDV) specific antigen in chicken tissues, embryos and allantoic fluid samples. Samples positive by virus isolation were also found positive by haemagglutination (HA) and haemagglutination inhibition (HI) tests and by dot-ELISA but negative samples were found negative by all the serological tests used. Dot-ELISA was able to detect 0.25-0.50 HA units of virus. The emerging utility of dot-ELISA for diagnosis of Newcastle disease virus infection has been discussed.
Roy
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Centre for Animal Health Studies, Tamilnadu Veterinary and Animal Sciences University, Madravaram Milk Colony, Madras, India.
Venugopalan
A T
AT
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006385:Hemagglutination Inhibition Tests / Q000662:veterinary; D006386:Hemagglutination Tests / Q000662:veterinary; D009521:Newcastle Disease / Q000175:diagnosis*; D009522:Newcastle disease virus / Q000276:immunology* / Q000302:isolation & purification
D000956:Antigens, Viral