Mutational analysis of the vacA promoter provides insight into gene transcription in Helicobacter pylori.
Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus -10 hexamer (TAtaaT) but little conservation of -35 sequences. In this study, mutations in either the H. pylori vacA -10 region or the -35 region resulted in decreased vacA transcription and suggested that an extended -10 motif is utilized. Thus, despite the lack of a -35 consensus sequence for H. pylori promoters, the -35 region plays a functional role in vacA transcription.
Forsyth
M H
MH
Departments of Medicine and Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2605, USA.
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D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D001483:Base Sequence; D016384:Consensus Sequence; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D016480:Helicobacter pylori / Q000235:genetics*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D011401:Promoter Regions, Genetic; D012808:Sigma Factor / Q000235:genetics; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D012808:Sigma Factor; C074689:VacA protein, Helicobacter pylori; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases