title: Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata).
abstract: We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDalpha and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.
authors: Department of Chemistry and Center for Molecular Catalysis, Seoul National University, Seoul 151-742, South Korea.
authors: D U
authors: DU
authors: Kim
authors: T Y
authors: TY
authors: Roh
authors: J
authors: J
authors: Lee
authors: J Y
authors: JY
authors: Noh
authors: Y J
authors: YJ
authors: Jang
authors: K L
authors: KL
authors: Hoe
authors: H S
authors: HS
authors: Yoo
authors: M U
authors: MU
authors: Choi
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D001937:Brassica / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D008969:Molecular Sequence Data; D010739:Phospholipase D / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment
chemical_list: D010739:Phospholipase D