title: Biotransformation of octane by E. coli HB101[pGEc47] on defined medium: octanoate production and product inhibition.
abstract: E. coli HB101[pGEc47], which is able to convert octane to octanoate, but cannot oxidize octanoate further, was grown on defined medium with glucose as carbon source in batch and continuous culture. The biomass yield on glucose decreased from 0.32 +/- 0.02 g g-1 in aqueous cultivations to 0.25 +/- 0.02 g g-1 in the presence of octane. Maximal octanoate productivities of 0.6 g L-1 h-1 were the same as found in cultivations on complex medium. The glucose-based carbon recovery in these experiments was 99 +/- 4% (in extreme, between 90% and 105%). An increase of the octane feed from 1% to 2% (v/v) or more led to washout of cells. This effect was reversible when the octane feed was decreased to its initial value of 1%. Analysis of experimental data by model simulation strongly suggested that washout was due to inhibition by octanoate only. Pulses of octanoate to a continuous culture grown on aqueous media were applied to analyze the inhibition further. Inhibition by acetate was not significant, but its presence in the medium reflected a physiological state that made the cells more sensitive to octanoate inhibition. Model simulation with linear inhibition kinetics could perfectly predict glucose consumption and the resulting glucose concentration. The linear type of inhibition was confirmed by a variety of batch experiments in the presence of different concentrations of octanoate. The glucose-based specific growth rate, mu, decreased linearly with increasing concentrations of octanoate and became zero at a threshold concentration pmax of 5.25 +/- 0.25 g L-1.
authors: Institute of Biotechnology, Swiss Federal Institute of Technology, ETH-Hoenggerberg/HPT, CH-8093 Zürich, Switzerland.
authors: S A
authors: SA
authors: Rothen
authors: M
authors: M
authors: Sauer
authors: B
authors: B
authors: Sonnleitner
authors: B
authors: B
authors: Witholt
mesh_terms: D019149:Bioreactors; D001711:Biotransformation; D002210:Caprylates / Q000378:metabolism* / Q000494:pharmacology; D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008954:Models, Biological; D009795:Octanes / Q000493:pharmacokinetics*
chemical_list: D002210:Caprylates; D003470:Culture Media; D009795:Octanes; D005947:Glucose; C031492:octanoic acid; C026728:octane