Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.
Tandemly repeated insertion sequence IS21, located on a suicide plasmid, promoted replicon fusion with bacteriophage lambda in vitro in the presence of ATP. This reaction was catalyzed in a cell extract containing the 45-kDa IstA protein (cointegrase) and the 30-kDa IstB helper protein of IS21 after both proteins had been overproduced in Escherichia coli. Without IstB, replicon fusion was inefficient and did not produce the 4-bp target duplications typical of IS21.
Schmid
S
S
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, CH-8092 Zürich, Switzerland.
Berger
B
B
Haas
D
D
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000502:physiology*; D010582:Bacteriophage lambda / Q000235:genetics*; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics; D012093:Replicon; D020080:Tandem Repeat Sequences
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; C118382:IstB protein, Pseudomonas aeruginosa