Sarin-like and soman-like organophosphorous agents activate PLCgamma in rat brains.
We report that there is a time-related change in the phospholipase C (PLC) activities of rat brain cytosol and membrane fractions after iv injection of a soman-like or a sarin-like organophosphorous agent (bis(isopropyl methyl)phosphonate [BIMP] and bis(pinacolyl methyl)phosphonate [BPMP]). PLCgamma was activated in the brain cytosol fraction from BPMP-injected rats. The phosphorylating activity of rat brain membrane fractions were enhanced by BPMP treatment. The brain membrane fractions from BPMP-treated rats phosphorylated several proteins, including supposedly PLCgamma in the brain cytosol fraction from control rats in vitro. These results suggest that soman and sarin may stimulate a membrane tyrosine kinase, including growth factor receptors, directly or indirectly.
Niijima
H
H
Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-0033, Japan.
Nagao
M
M
Nakajima
M
M
Takatori
T
T
Matsuda
Y
Y
Iwase
H
H
Kobayashi
M
M
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001921:Brain / Q000187:drug effects* / Q000201:enzymology; D002619:Chemical Warfare Agents / Q000633:toxicity*; D004789:Enzyme Activation / Q000187:drug effects; D008297:Male; D009943:Organophosphorus Compounds / Q000633:toxicity*; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D012524:Sarin / Q000633:toxicity*; D012999:Soman / Q000633:toxicity*; D010738:Type C Phospholipases / Q000378:metabolism*
D002619:Chemical Warfare Agents; D009943:Organophosphorus Compounds; D012999:Soman; D012524:Sarin; D010738:Type C Phospholipases