Genetic organization of the Escherichia coli K10 capsule gene cluster: identification and characterization of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions.
Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K10 capsule production, suggesting that expression of the K10 capsule was regulated by RfaH in a manner analogous to group II capsule gene clusters. An IS3 element and a phiR73-like prophage, both of which may have played a role in the acquisition of group III capsule gene clusters, were detected flanking the K10 capsule genes.
Clarke
B R
BR
School of Biological Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
Pearce
R
R
Roberts
I S
IS
D000595:Amino Acid Sequence; D016667:Bacterial Capsules / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D017124:Conserved Sequence; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D029968:Escherichia coli Proteins; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D010445:Peptide Elongation Factors / Q000235:genetics; D011134:Polysaccharides / Q000378:metabolism; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
D004269:DNA, Bacterial; D029968:Escherichia coli Proteins; D010445:Peptide Elongation Factors; D011134:Polysaccharides; C106401:RfaH protein, E coli; D015534:Trans-Activators