Identification of the cause of a brain abscess by direct 16S ribosomal DNA sequencing.
We report the case of a young man who apparently suffered successive episodes of meningitis and cerebral abscess over a 1-month period, both of which were diagnosed by two different molecular approaches; PCR for Neisseria meningitidis IS1106 from CSF and 16S rRNA gene sequencing on a specimen of brain pus. In each case, cultures were negative due to prior antibiotic therapy.
Logan
J M
JM
Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital, UK.
Orange
G V
GV
Maggs
A F
AF
D000328:Adult; D001922:Brain Abscess / Q000175:diagnosis / Q000235:genetics / Q000382:microbiology*; D004269:DNA, Bacterial; D006801:Humans; D008297:Male; D008585:Meningitis, Meningococcal / Q000175:diagnosis / Q000235:genetics; D009345:Neisseria meningitidis / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods; D013045:Species Specificity; D013492:Suppuration / Q000175:diagnosis / Q000382:microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S