Isolation and characterization of monoclonal antibodies directed against murine FRP-1/CD98/4F2 heavy chain: murine FRP-1 is an alloantigen and amino acid change at 129 (P<-->R) is related to the alloantigenicity.
Nineteen mAb directed against murine fusion regulatory protein-1 (mFRP-1)/4F2/CD98 were isolated and their biological properties were analysed. Intriguingly, mFRP-1 was found to be an alloantigen, namely, FRP-1.1 (DBA/2 and CBA mice type) and FRP-1.2 (BALB/c, C57BL/6 and C3H/He mice type). The nucleotide sequences of FRP-1.1 and FRP-1.2 were determined, demonstrating that amino acid change at 129 (P<-->R) is related to the alloantigenicity. mFRP-1 is expressed on thymocytes, on spleen cells, on peripheral lymphocytes and on blood monocytes, suggesting that the physiological role in vivo of murine FRP-1 is different from that of human FRP-1. The biological activities of antimFRP-1 mAbs showed by the present study are: (i) enhancement of Newcastle disease virus-induced cell fusion; (ii) suppression of HIVgp160-mediated cell fusion; and (iii) induction of aggregation and multinucleated giant cells of monocytes/macrophages.
Tsumura
H
H
Department of Microbiology, Mie University School of Medicine, Mie-ken, Japan. tsumura@doc.medic.mie-u.ac.jp
Kawano
M
M
Tajima
M
M
Kusaura
T
T
Kozuka
Y
Y
Yoshimura
S
S
Komada
H
H
Tsurudome
M
M
Nishio
M
M
Kusagawa
S
S
Shimura
K
K
Ito
Y
Y
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D000911:Antibodies, Monoclonal / Q000302:isolation & purification*; D015703:Antigens, CD / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001483:Base Sequence; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D002449:Cell Aggregation / Q000276:immunology; D002459:Cell Fusion; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D027261:Fusion Regulatory Protein-1; D015726:Giant Cells / Q000276:immunology; D019302:HIV Envelope Protein gp160 / Q000276:immunology; D006801:Humans; D007519:Isoantigens / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000276:immunology / Q000472:pathogenicity; D017386:Sequence Homology, Amino Acid
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D002352:Carrier Proteins; D017931:DNA Primers; D027261:Fusion Regulatory Protein-1; D019302:HIV Envelope Protein gp160; D007519:Isoantigens