Differential regulation of protooncogene c-myc expression in rat ventral prostate after castration.
To evaluate the possibility that the protooncogene c-myc plays a role in ventral prostate, the effects of castration have been investigated at a beginning of a period by Northern blot hybridization and the levels of c-myc mRNA were also compared with mRNA of androgen-regulated genes, C1 and TRPM-2. Levels of c-myc mRNA in ventral prostate increased with maximal stimulation reached at 6 hours (early induction) and 48 hours (late induction) after castration, respectively. The level of C1 mRNA did not change and TRPM-2 was not detected at early induction of c-myc mRNA after castration. The level of early induction of c-myc mRNA after castration was increased in ventral prostate treated with cycloheximide, but it was almost reduced by actinomycin-D pretreatment. Administration of androgen at the time of castration prevented early induction of c-myc mRNA. These results suggest that protooncogene c-myc is differentially regulated in ventral prostate after castration.
Lee
J H
JH
Department of Biochemistry, College of Medicine, Chungnam National University, Daejeon, Korea.
Sul
C K
CK
Kim
Y K
YK
Hwang
B D
BD
Lim
K
K
D000818:Animals; D015152:Blotting, Northern; D002369:Castration; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005786:Gene Expression Regulation; D016259:Genes, myc; D008297:Male; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013739:Testosterone / Q000494:pharmacology; D013997:Time Factors
D003609:Dactinomycin; D013739:Testosterone; D003513:Cycloheximide