Interaction of purified human proteinase 3 (PR3) with reconstituted lipid bilayers.
Proteinase 3 (PR3), the major target autoantigen in Wegener's granulomatosis is a serine proteinase that is normally stored intracellularly in the primary granules of quiescent neutrophils and monocytes. Upon cell activation, a significant portion of this antigen is detected on the cell surface membrane. The nature of the association of PR3 with the membrane and its functional significance are unknown. We investigated the interaction of purified human PR3 with mixtures of zwitterionic (dimyristoyl-L-alpha-phosphatidylcholine, DMPC) and anionic (dimyristoyl-L-alpha-phosphatidylglycerol, DMPG) phospholipids in reconstituted lipid bilayers using differential scanning calorimetry and lipid photolabeling, and measured the affinity of this interaction using spectrophotometry. Two other primary granule constituents, human neutrophil elastase (HNE) and myeloperoxidase (MPO) were investigated for comparison. In calorimetric assays, using lipid vesicles of mixed DMPC/DMPG, increasing PR3 concentrations (protein/lipid molar ratio from 0 to 1 : 110) induced a significant decrease of the main chain transition enthalpy and a shift in chain melting temperatures which is indicative of partial insertion of PR3 into the hydrophobic region of the lipid membranes. This was confirmed by hydrophobic photolabeling using liposomes containing trace amounts of the photoactivable [125I]-labeled phosphatidylcholine analog TID-PC/16. The molar affinity of PR3, HNE, and MPO to lipid vesicles of different DMPC/DMPG ratios was then determined by spectrophotometry. At a DMPC/DMPG ratio of 1 : 1, molar affinities of PR3, Kd = 4.5 +/- 0.3 microm; HNE, 14.5 +/- 1.2 microm; and MPO, 50 +/- 5 microm (n = 3) were estimated. The lipid-associated PR3 exhibited two-fold lower Vmax and Km values, and its enzyme activity was slightly more inhibited (Ki) by the natural alpha1-proteinase inhibitor (alpha1-PI) or an autoantibody to PR3.
Goldmann
W H
WH
Leukocyte Biology and Inflammation Program, Rental Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA. goldmann_w@hub.tch.harvard.edu
Niles
J L
JL
Arnaout
M A
MA
D000345:Affinity Labels; D001324:Autoantigens / Q000737:chemistry / Q000302:isolation & purification; D002152:Calorimetry, Differential Scanning; D014890:Granulomatosis with Polyangiitis / Q000201:enzymology / Q000276:immunology; D006801:Humans; D066298:In Vitro Techniques; D019272:Leukocyte Elastase / Q000276:immunology; D008051:Lipid Bilayers; D008958:Models, Molecular; D053146:Myeloblastin; D009195:Peroxidase / Q000276:immunology; D011487:Protein Conformation; D012697:Serine Endopeptidases / Q000737:chemistry / Q000276:immunology* / Q000302:isolation & purification*; D013053:Spectrophotometry
D000345:Affinity Labels; D001324:Autoantigens; D008051:Lipid Bilayers; D009195:Peroxidase; D012697:Serine Endopeptidases; D019272:Leukocyte Elastase; D053146:Myeloblastin