Influenza A virus accelerates neutrophil apoptosis and markedly potentiates apoptotic effects of bacteria.
Neutrophils are recruited into the airway in the early phase of uncomplicated influenza A virus (IAV) infection and during the bacterial superinfections that are a significant cause of morbidity and mortality in IAV-infected subjects. In this report, we show that IAV accelerates neutrophil apoptosis. Unopsonized Escherichia coli had similar effects, although apoptotic effects of opsonized E coli were greater. When neutrophils were treated with both IAV and unopsonized E coli, a marked enhancement of the rate and extent of neutrophil apoptosis occurred as compared with that caused by either pathogen alone. Treatment of neutrophils with IAV markedly increased phagocytosis of E coli. Simultaneous treatment of neutrophils with IAV and E coli also elicited greater hydrogen peroxide production than did either pathogen alone. IAV increased neutrophil expression of Fas antigen and Fas ligand, and it also increased release of Fas ligand into the cell supernatant. These findings may have relevance to the understanding of inflammatory responses to IAV in vivo and of bacterial superinfection of IAV-infected subjects.
Colamussi
M L
ML
Department of Medicine and Pathology, Boston University School of Medicine, Boston, MA 02118, USA.
White
M R
MR
Crouch
E
E
Hartshorn
K L
KL
D017209:Apoptosis; D001424:Bacterial Infections / Q000150:complications / Q000276:immunology; D002478:Cells, Cultured; D004926:Escherichia coli / Q000276:immunology*; D053222:Fas Ligand Protein; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D009980:Influenza A virus / Q000502:physiology*; D007251:Influenza, Human / Q000276:immunology*; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000382:microbiology / Q000502:physiology / Q000821:virology*; D009895:Opsonin Proteins / Q000276:immunology; D010587:Phagocytosis; D016897:Respiratory Burst; D015163:Superinfection; D019014:fas Receptor / Q000096:biosynthesis
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D008562:Membrane Glycoproteins; D009895:Opsonin Proteins; D019014:fas Receptor; D006861:Hydrogen Peroxide