title: The structure of the carbohydrate backbone of the core-lipid A region of the lipopolysaccharide from a clinical isolate of Yersinia enterocolitica O:9.
abstract: Yersinia enterocolitica O:9 strain Ruokola/71-c-PhiR1-37-R possesses mainly rough-type lipopolysaccaride (LPS) and smaller amounts of S-form LPS. Structural analysis of the former is reported here. After deacylation of the LPS, the phosphorylated carbohydrate backbone of the inner core-lipid A region could be isolated by using high-performance anion-exchange chromatography. Its structure was determined by means of compositional and methylation analyses and 1H-, 13C-, and 31P-NMR spectroscopy as: [see text] in which L-alpha-D-Hep is L-glycero-alpha-D-manno-heptopyranose, D-alpha-D-Hep is D-glycero-alpha-D-manno-heptopyranose, and Kdo is 3-deoxy-D-manno-oct-2-ulopyranosonic acid. All hexoses are pyranoses.
authors: Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Germany.
authors: S
authors: S
authors: Müller-Loennies
authors: S
authors: S
authors: Rund
authors: E
authors: E
authors: Ervelä
authors: M
authors: M
authors: Skurnik
authors: O
authors: O
authors: Holst
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D006801:Humans; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012703:Serotyping; D015008:Yersinia enterocolitica / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification
chemical_list: D002241:Carbohydrates; D008050:Lipid A; D008070:Lipopolysaccharides