The structure of the carbohydrate backbone of the core-lipid A region of the lipopolysaccharide from a clinical isolate of Yersinia enterocolitica O:9.
Yersinia enterocolitica O:9 strain Ruokola/71-c-PhiR1-37-R possesses mainly rough-type lipopolysaccaride (LPS) and smaller amounts of S-form LPS. Structural analysis of the former is reported here. After deacylation of the LPS, the phosphorylated carbohydrate backbone of the inner core-lipid A region could be isolated by using high-performance anion-exchange chromatography. Its structure was determined by means of compositional and methylation analyses and 1H-, 13C-, and 31P-NMR spectroscopy as: [see text] in which L-alpha-D-Hep is L-glycero-alpha-D-manno-heptopyranose, D-alpha-D-Hep is D-glycero-alpha-D-manno-heptopyranose, and Kdo is 3-deoxy-D-manno-oct-2-ulopyranosonic acid. All hexoses are pyranoses.
Müller-Loennies
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S
Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Germany.
Rund
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S
Ervelä
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E
Skurnik
M
M
Holst
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O
D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000737:chemistry / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D006801:Humans; D008050:Lipid A / Q000737:chemistry* / Q000302:isolation & purification; D008070:Lipopolysaccharides; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D012703:Serotyping; D015008:Yersinia enterocolitica / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification
D002241:Carbohydrates; D008050:Lipid A; D008070:Lipopolysaccharides