Chromosome abnormalities in human embryos.
The presence of numerical chromosome abnormalities in human embryos was studied using fluorescence in-situ hybridization with four or more chromosome-specific probes. When most cells of an embryo are analysed, this technique allows differentiation to be made between aneuploidy, mosaicism, haploidy and polyploidy. Abnormal types of fertilization, such as unipronucleated, tripronucleated zygotes and zygotes with uneven pronuclei, were studied using this technique. We have found a strong correlation between some types of dysmorphism with chromosomal abnormalities. In addition, the more impaired the development of an embryo, the more chromosomal abnormalities were detected in those embryos. Maternal age and other factors were linked to an increase in chromosome abnormalities (hormonal regimes, temperature changes), but not to intracytoplasmic sperm injection.
Munné
S
S
The Center for Reproductive Medicine and Science of Saint Barnabas Medical Center, New Jersey, USA.
Cohen
J
J
D000782:Aneuploidy; D002467:Cell Nucleus / Q000648:ultrastructure; D002869:Chromosome Aberrations; D002970:Cleavage Stage, Ovum; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D006801:Humans; D007621:Karyotyping; D009030:Mosaicism; D015053:Zygote / Q000648:ultrastructure