Growth characteristics of Escherichia coli HB101[pGEc47] on defined medium.
This paper shows that differences in growth behavior of Escherichia coli strain HB101 and strain HB101[pGEc47] can be related to yeast extract-enriched medium rather than plasmid properties. An optimal medium for growth of E. coli HB101[pGEc47] was designed based on the individual yield coefficients for specific medium components (NH4+ 6 g g-1, PO43- 14 g g-1, SO42- 50 g g-1). The yield coefficient for L-leucine depends on the glucose content of the medium (20 g g-1 for 3% glucose, 40 g g-1 for 1% glucose) and the yield coefficient for L-proline depends on the cultivation mode (20 g g-1 for batch cultivation, 44 g g-1 for continuous cultivation). Growth on defined medium after medium optimization is as rapid as on complex medium (0. 42-0.45 h-1). The critical dilution rate (DR) in the defined medium above which undesired production of acetic acid occurs is in the range of 0.23-0.26 h-1.
Rothen
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Institute of Biotechnology, Swiss Federal Institute of Technology, ETH-Hoenggerberg/HPT, CH-8093 Zürich.
Sauer
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Sonnleitner
B
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Witholt
B
B
D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development*; D007930:Leucine; D008670:Metals; D011392:Proline; D011995:Recombination, Genetic; D014815:Vitamins; D015003:Yeasts
D003470:Culture Media; D008670:Metals; D014815:Vitamins; D011392:Proline; D007930:Leucine