The effect of 1,3-dinitrobenzene on the functioning of the auditory pathway in the rat.
1,3-Dinitrobenzene (DNB) has previously been shown to be neuropathic, causing gliovascular lesioning in the rat brainstem, with the nuclei of the auditory pathway being particularly affected. Lesion severity was shown to be dependent on functional activity, which could be markedly decreased within one pathway by monaurally reducing sensory input. The aim of this study was to characterise the changes in electrophysiological and vascular function associated with this asymmetric lesioning. Depth electrodes located in the inferior colliculi were used to measure wave II and IV of the auditory evoked response (AER) and collicular blood flow. These were measured up to eight days after DNB exposure in rats, in which preexisting reduction in sensory input in one ear was achieved by tympanic membrane rupture. Significant increases of between 14-27 dB were seen in the mean stimulus level required to generate a 50% isoamplitude response for wave IV in the intact (ie vulnerable) pathway over days 1-8 post DNB. No significant changes in this response for the other AER waves were seen over the same recording period. Significant increases in blood flow were seen in the inferior colliculi up to 24 hours after the final dose of DNB. Differences in increased flow between the colliculi were also highly significant, with peak increases of 200% and 80% seen in the intact and protected sides respectively. This difference shows that DNB enhanced blood flow appears to reflect the severity of the DNB induced functional deficit. In both cases, disturbance to normal glial function in maintaining K+ homeostasis, may underlie the neurophysiological deficit and the increase in blood flow seen at the level of the inferior colliculi. These asymmetric functional changes were also parallelled by the differential lesion severity between the protected and unprotected pathways. Hence, protection against DNB glial lesion severity by reduction in sensory input, and consequently metabolic demand, is paralleled by the early vascular response and functional neuronal deficit seen over the eight day post DNB recording period.
Mulheran
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M
MRC Toxicology Unit, University of Leicester, UK. mm22@le.ac.uk
Ray
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DE
Lister
T
T
Nolan
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CC
D000161:Acoustic Stimulation; D000818:Animals; D001306:Auditory Pathways / Q000033:anatomy & histology / Q000187:drug effects*; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004136:Dinitrobenzenes / Q000633:toxicity*; D004594:Electrophysiology; D005072:Evoked Potentials, Auditory / Q000187:drug effects; D007245:Inferior Colliculi / Q000033:anatomy & histology / Q000098:blood supply / Q000187:drug effects; D008297:Male; D051381:Rats; D011916:Rats, Inbred F344; D018058:Tympanic Membrane Perforation / Q000503:physiopathology
D004136:Dinitrobenzenes; C017906:3-dinitrobenzene