Sonodynamically induced effect of rose bengal on isolated sarcoma 180 cells.
OBJECTIVE
The ultrasonically induced effect of rose bengal (RB) on isolated tumor cells was investigated.


METHODS
Sarcoma 180 cells were suspended in air-saturated phosphate-buffered saline and exposed to ultrasound in standing wave mode for up to 60 s in the presence and absence of RB. Cell viability was determined by the ability to exclude trypan blue.


RESULTS
The rate of inducing cell damage by ultrasound was enhanced two to three times with 160 microM RB. while no cell damage was observed with RB alone. This enhancement was significantly inhibited by histidine.


CONCLUSIONS
Ultrasonically induced in vitro cell damage was significantly enhanced by RB. A sonochemical mechanism may be suggested since the enhancement was significantly inhibited by an active oxygen scavenger.
Umemura
S
S
Central Research Laboratory, Hitachi, Ltd., Tokyo, Japan.
Yumita
N
N
Umemura
K
K
Nishigaki
R
R
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D004336:Drug Antagonism; D005456:Fluorescent Dyes / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012395:Rose Bengal / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000473:pathology*; D014343:Trypan Blue; D014465:Ultrasonics
D004396:Coloring Agents; D005456:Fluorescent Dyes; D012395:Rose Bengal; D006639:Histidine; D014343:Trypan Blue