Amperometric determination of lysine using a lysine oxidase biosensor based on rigid-conducting composites.
In this study, amperometric biosensors based on rigid conducting composites are developed for the determination of lysine. These lysine biosensors consist of chemically immobilized lysine oxidase membranes attached to either graphite-methacrylate or peroxidase-modified graphite-methacrylate electrodes. The enzymatic degradation of lysine releases hydrogen peroxide, which is the basis of the amperometric detection. The direct oxidation of hydrogen peroxide is monitored at +1000 mV with a graphite-methacrylate electrode, while with the peroxidase-modified electrode reductive detection is performed. In addition, for the peroxidase-modified biocomposite electrode, both direct electron transfer and hydroquinone-mediated detection are studied. For the lysine biosensor based on the hydroquinone-mediated peroxidase biocomposite, the linear range is up to 1.6 x 10(-4) M, the sensitivity 11300 microA/M, the repeatability 1.8%, the detection limit 8.2 x 10(-7) M and the response time t95% is 42 s. The proposed biosensors are used to determine lysine in pharmaceutical samples. Results are consistent with those obtained with the standard method.
Saurina
J
J
Department of Chemistry, Autonomous University of Barcelona, Bellaterra, Spain.
Hernández-Cassou
S
S
Alegret
S
S
Fàbregas
E
E
D015374:Biosensing Techniques; D004563:Electrochemistry; D004795:Enzyme Stability; D006863:Hydrogen-Ion Concentration; D008239:Lysine / Q000032:analysis*; D008249:Protein-Lysine 6-Oxidase / Q000378:metabolism*; D013696:Temperature
D008249:Protein-Lysine 6-Oxidase; D008239:Lysine