title: Smooth-muscle myosin heavy-chain SM-B isoform expression in developing and adult rat lung.
abstract: The smooth-muscle cells composing the vasculature and airways of the lung display a variety of contractile protein phenotypes. To date, however, it has remained unclear how these phenotypes might contribute differentially to contractile activity. To address this issue, we made monospecific rabbit polyclonal antibodies against the difference peptide for the SM-B smooth-muscle myosin heavy chain (SMMHC) and used these to investigate the distribution of the SM-B isoform in lung. SM-B has a seven-amino acid insert in the head region that is known to result in a higher actin-activated adenosine triphosphatase activity and in vitro motility. During development, reactivity is first seen in the trachea and bronchi of saccular lung at the time of birth, when other SMMHC isoforms also are present. Immunoreactivity spreads distally through the airways as development proceeds, reaching the level of alveolar septae in the adult. Although the smaller vessels of the pulmonary vasculature react strongly with the SM-B antibody, reactivity is infrequently observed in large pulmonary vessels. Adult tracheal smooth muscle is highly and more uniformly reactive, commensurate with its relatively high maximal velocity of shortening. The differential expression of the SM-B isoform in vascular and airway smooth muscles demonstrated in this study may provide the molecular basis for functional differences between these smooth-muscle cell types and may provide one mechanism for adapting contractility in response to physiologic stresses in the lung.
authors: Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, USA.
authors: R B
authors: RB
authors: Low
authors: J
authors: J
authors: Mitchell
authors: J
authors: J
authors: Woodcock-Mitchell
authors: A S
authors: AS
authors: Rovner
authors: S L
authors: SL
authors: White
mesh_terms: D000375:Aging / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies; D005314:Embryonic and Fetal Development; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D008168:Lung / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D024510:Muscle Development; D009130:Muscle, Smooth / Q000196:embryology / Q000254:growth & development / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000196:embryology / Q000254:growth & development / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000032:analysis / Q000235:genetics*; D020033:Protein Isoforms / Q000032:analysis / Q000235:genetics; D011652:Pulmonary Circulation; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D014132:Trachea / Q000378:metabolism
chemical_list: D000906:Antibodies; D020033:Protein Isoforms; D018995:Myosin Heavy Chains