A novel biotinylated degradable polymer for cell-interactive applications.
We describe the development of a novel biodegradable polymer designed to present bioactive motifs at the surfaces of materials of any architecture. The polymer is a block copolymer of biotinylated poly(ethylene glycol) (PEG) with poly(lactic acid) (PLA); it utilizes the high-affinity coupling of the biotin-avidin system to undergo postfabrication surface engineering. We show, using surface plasmon resonance analysis (SPR) and confocal microscopy that surface engineering can be achieved under aqueous conditions in short time periods. These surfaces interact with cell surface molecules and generate beneficial responses as demonstrated by the model study of integrin-mediated spreading of endothelial cells on polymer surfaces presenting RGD peptide adhesion sequences.
Cannizzaro
S M
SM
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Padera
R F
RF
Langer
R
R
Rogers
R A
RA
Black
F E
FE
Davies
M C
MC
Tendler
S J
SJ
Shakesheff
K M
KM
D000818:Animals; D001011:Aorta; D001672:Biocompatible Materials; D019921:Biotinylation; D002417:Cattle; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002465:Cell Movement; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology* / Q000502:physiology; D016650:Fluorescein-5-isothiocyanate; D007773:Lactates; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011092:Polyethylene Glycols; D011108:Polymers; D013499:Surface Properties
D001672:Biocompatible Materials; D007773:Lactates; D011108:Polymers; C058523:polyethylene oxide-polylactic acid block copolymer; D011092:Polyethylene Glycols; D016650:Fluorescein-5-isothiocyanate