Use of green fluorescent protein to detect expression of an endopolygalacturonase gene of Colletotrichum lindemuthianum during bean infection.
The 5' noncoding region of clpg2, an endopolygalacturonase gene of the bean pathogen Colletotrichum lindemuthianum, was fused to the coding sequence of a gene encoding a green fluorescent protein (GFP), and the construct was introduced into the fungal genome. Detection of GFP accumulation by fluorescence microscopy examination revealed that clpg2 was expressed at the early stages of germination of the conidia and during appressorium formation both in vitro and on the host plant.
Dumas
B
B
UMR 5546 CNRS-UPS, "Signaux et Messages Cellulaires chez les Végétaux," Pôle de Biotechnologie Végétale, Auzeville 31326, Castanet-Tolosan, France. dumas@cict.fr
Centis
S
S
Sarrazin
N
N
Esquerré-Tugayé
M T
MT
D020231:Colletotrichum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D007887:Fabaceae / Q000382:microbiology*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D010946:Plants, Medicinal; D011096:Polygalacturonase / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D020133:Reverse Transcriptase Polymerase Chain Reaction
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins; D011096:Polygalacturonase