title: Role of Nr13 in regulation of programmed cell death in the bursa of Fabricius.
abstract: Apoptotic cell death is developmentally regulated in the chicken bursa of Fabricius. Although apoptosis is low in the embryonic bursa, cell death increases markedly after hatching. The expression of Bcl2 family cell death antagonists was examined to identify the genes that regulate bursal cell apoptosis. The expression of Bcl-xL, A1, and Mcl1 was detected in both embryos and hatched birds, whereas Nr13 was expressed at high levels in embryonic bursa, and decreased significantly after hatching, correlating inversely with apoptosis. The oncogene v-reland phorbol myristate acetate, two known inhibitors of bursal cell apoptosis, induced Nr13 expression. Overexpression of Nr13 in DT40 bursal lymphoma cells protected them from low serum-induced apoptosis. The mechanism of inhibition of apoptosis by Nr13 is likely to involve a critical BH4 domain and interaction with death agonist Bax. Deletion of the BH4 domain converted Nr13 into a death agonist. Bax coimmunoprecipitated with Nr13 and Bax was induced, whereas Nr13 levels diminished when bursal lymphoblasts were induced to apoptosis by dispersion. Bursal transplantation studies demonstrated that Nr13 could prevent the in vivo programmed elimination of bursal stem cells after hatching, suggesting that Nr13 plays a role in maintaining bursal stem cells.
authors: Fred-Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
authors: R M
authors: RM
authors: Lee
authors: G
authors: G
authors: Gillet
authors: J
authors: J
authors: Burnside
authors: S J
authors: SJ
authors: Thomas
authors: P
authors: P
authors: Neiman
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D030161:Avian Proteins; D001402:B-Lymphocytes / Q000166:cytology; D002060:Bursa of Fabricius / Q000166:cytology* / Q000378:metabolism; D002470:Cell Survival / Q000502:physiology; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology; D008969:Molecular Sequence Data; D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D015682:Retroviridae Proteins, Oncogenic / Q000502:physiology; D013234:Stem Cells / Q000166:cytology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D051028:bcl-2-Associated X Protein
chemical_list: D030161:Avian Proteins; C118281:Nr13 protein, chicken; D020572:Oncogene Proteins v-rel; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D015682:Retroviridae Proteins, Oncogenic; D051028:bcl-2-Associated X Protein; D013755:Tetradecanoylphorbol Acetate