Brasilicardin A, a new terpenoid antibiotic from pathogenic Nocardia brasiliensis: fermentation, isolation and biological activity.
A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC50 value was 0.057 microg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.
Komaki
H
H
Research Laboratory, Higeta Shoyu Co., Ltd., Choshi, Chiba, Japan.
Nemoto
A
A
Tanaka
Y
Y
Takagi
H
H
Yazawa
K
K
Mikami
Y
Y
Shigemori
H
H
Kobayashi
J
J
Ando
A
A
Nagata
Y
Y
D000617:Aminoglycosides; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D005658:Fungi / Q000187:drug effects; D006801:Humans; D020104:Immunophilins / Q000037:antagonists & inhibitors; D007166:Immunosuppressive Agents / Q000302:isolation & purification / Q000494:pharmacology*; D007376:Interleukin-2 / Q000378:metabolism; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008826:Microbial Sensitivity Tests; D009615:Nocardia / Q000378:metabolism*
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D007376:Interleukin-2; C118259:brasilicardin A; D020104:Immunophilins