title: Detection of various epitopes of murine osteopontin by monoclonal antibodies.
abstract: We immunized rats with recombinant murine osteopontin protein and obtained four monoclonal antibodies recognizing distinct epitopes of murine osteopontin. OPN1.2 recognized the amino-terminal half of OPN, while OPN2.2, OPN2.3, and OPN3.1 recognized the carboxy-terminal half of OPN. The epitope recognized by OPN2.2 was destroyed by further cleavage of the carboxy half of OPN. The epitope recognized by OPN2.3 was located in the amino-terminal end of the carboxy half of OPN, whereas that recognized by OPN3.1 was located in the carboxy-terminal end of the carboxy half of OPN. OPN1.2 and OPN2.2 recognized thrombin-cleaved osteopontin, whereas thrombin-cleaved osteopontin was not recognized by OPN2.3 and OPN3.1. Thus, these monoclonal antibodies will be useful in structure/function studies of the role of osteopontin in murine models of disease.
authors: Institute of Immunological Science, Hokkaido University, Kita-15, Niishi-7, Kita-ku, Sapporo, Japan.
authors: H
authors: H
authors: Hotta
authors: S
authors: S
authors: Kon
authors: Y U
authors: YU
authors: Katagiri
authors: N
authors: N
authors: Tosa
authors: T
authors: T
authors: Tsukamoto
authors: A F
authors: AF
authors: Chambers
authors: T
authors: T
authors: Uede
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D015153:Blotting, Western; D002113:Calcification, Physiologic; D000939:Epitopes / Q000032:analysis / Q000276:immunology*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009154:Mutation; D009206:Myocardium / Q000737:chemistry / Q000166:cytology; D009842:Oligopeptides / Q000235:genetics / Q000276:immunology; D053495:Osteopontin; D010446:Peptide Fragments / Q000235:genetics / Q000276:immunology; D011487:Protein Conformation; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D017384:Sequence Deletion; D012795:Sialoglycoproteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013917:Thrombin / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D009842:Oligopeptides; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; C506897:Spp1 protein, rat; D053495:Osteopontin; C047981:arginyl-glycyl-aspartic acid; D013917:Thrombin