Molecular typing for HLA class I using ARMS-PCR: further developments following the 12th International Histocompatibility Workshop.
Molecular typing for HLA class I was introduced in the 12th International Histocompatibility Workshop. Following a pilot study using three methods, sequence specific oligotyping (SSO), reverse dot blot and amplification refractory mutation system (ARMS)-PCR, the ARMS-PCR method was selected for use. A great advantage of an ARMS-PCR method is that, unlike the other two methods, it can determine whether sequence motifs are in cis or in trans, as ARMS-PCR detects two cis located motifs per reaction using forward and reverse sequence specific primers. Resolution was designed to be low to medium level for HLA-A, -B and -C alleles. Two hundred and fifty class I kits and 83 HLA-A2 subtyping kits were distributed. The A2 subtyping kit used a two round nested PCR system to identify all of the A2 alleles known at the time. Typing results on control DNA samples distributed with both the kits showed a very satisfactory performance. Since the 12th Workshop, the kits have been developed with the addition of new primers and primer mixes to increase the resolution of the test.
Tonks
S
S
Cancer and Immunogenetics Laboratory, Imperial Cancer Research Fund, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Marsh
S G
SG
Bunce
M
M
Bodmer
J G
JG
D004247:DNA; D004493:Education; D015234:HLA-A Antigens / Q000145:classification / Q000235:genetics*; D015235:HLA-B Antigens / Q000145:classification / Q000235:genetics*; D015236:HLA-C Antigens / Q000145:classification / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008285:Major Histocompatibility Complex; D016133:Polymerase Chain Reaction / Q000379:methods*
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015236:HLA-C Antigens; D004247:DNA