A cardioactive peptide from the southern armyworm, Spodoptera eridania.
A cardioactive peptide was isolated from extracts of whole heads of the southern armyworm, Spodoptera eridania. This peptide has the sequence ENFAVGCTPGYQRTADGRCKPTF (Mr = 2516.8), determined from both Edman sequencing and tandem mass spectrometry in combination with off-line micropreparative capillary liquid chromatography. This peptide, termed Spoer-CAP23, has excitatory effects on a semi-isolated heart from larval Manduca sexta, causing an inotropic effect at low concentrations of peptide and chronotropic and inotropic effects at high doses. The threshold concentration for stimulatory effects of the synthetic peptide on the semi-isolated heart was about 1 nM, suggesting a physiological role as a neuropeptide.
Furuya
K
K
Genentech, Inc., South San Francisco, CA 94080, USA.
Hackett
M
M
Cirelli
M A
MA
Schegg
K M
KM
Wang
H
H
Shabanowitz
J
J
Hunt
D F
DF
Schooley
D A
DA
D000595:Amino Acid Sequence; D000818:Animals; D006321:Heart / Q000187:drug effects* / Q000502:physiology; D018402:Manduca / Q000187:drug effects / Q000502:physiology; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D017421:Sequence Analysis; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera / Q000737:chemistry*
D009479:Neuropeptides; D009842:Oligopeptides; C119584:Spoer-CAP(23)