title: Refinement of the LOH region 1 at 11q23.1 deleted in human breast carcinomas and sublocalization of 11 expressed sequence tags within the refined region.
abstract: Loss of constitutive heterozygosity at 11q23 has been detected in various human solid tumors. Here, we described the analysis of a series of normal and tumor pairs from 110 breast carcinomas for the presence of loss of heterozygosity at 11q23 loci. The overall frequency of LOH was 48%, confirming the importance of deletions at 11q23 in breast tumorigenesis. Previously, we have identified two independent regions of LOH at 11q23, the LOH region 1 at 11q23.1 and the LOH region 2 at 11q23.3. The most telomeric region was recently refined between loci D11S1345 and D11S1316, a region of about 1 Mb. However, the LOH region 1, most centromeric, was still not finely refined: the boundaries were defined by loci D11S2000 and D11S897, separated by about 8 Mb. Here, we refined its boundaries between loci D11S1347 and D11S927, a region of about 2 Mb. We have mapped 11 expressed sequence tags (ESTs) within this region and excluded another 20. This study represents a further step toward the identification of the putative tumor suppressor gene found within the LOH region 1 at 11q23.1.
authors: Department of Molecular and Diagnostic Medicine, University of Ferrara, Italy.
authors: M G
authors: MG
authors: di Iasio
authors: G
authors: G
authors: Calin
authors: M G
authors: MG
authors: Tibiletti
authors: I
authors: I
authors: Vorechovsky
authors: K P
authors: KP
authors: Benediktsson
authors: R
authors: R
authors: Taramelli
authors: G
authors: G
authors: Barbanti-Brodano
authors: M
authors: M
authors: Negrini
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics*; D002874:Chromosome Mapping; D002880:Chromosomes, Human, Pair 11 / Q000235:genetics* / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000235:genetics; D020224:Expressed Sequence Tags; D005260:Female; D006801:Humans; D019656:Loss of Heterozygosity; D017384:Sequence Deletion
chemical_list: D004273:DNA, Neoplasm