Iodo-QNB cortical binding and brain perfusion: effects of a cholinergic basal forebrain lesion in the rat.
This study deals with the question of whether in vivo application of [125I]iodo-quinuclidinyl-benzilate (QNB) is able to demonstrate changes in cortical muscarinic receptor density induced by a cholinergic immunolesion of the rat basal forebrain cholinergic system, and whether the potential effects on IQNB distribution in vivo are also associated with effects on regional cerebral perfusion. Immunolesioned and control animals were injected with (R,S) [125]iodo-QNB and with [99mTc]-d,l-hexamethylpropyleneamine oxime (HMPAO). The cerebral distribution of both tracers was imaged using double tracer autoradiography. Impaired cholinergic transmission was paralleled by a 10-15% increase of [125I]iodo-QNB binding in the regions of cortex and hippocampus. The local cerebral blood flow remained unchanged after cholinergic lesion.
Sorger
D
D
Department of Nuclear Medicine, University of Leipzig, Germany. SORD@server3.medizin.uni-leipzig.de
Kämpfer
I
I
Schliebs
R
R
Rossner
S
S
Dannenberg
C
C
Knapp
W H
WH
D000109:Acetylcholine / Q000502:physiology*; D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D017763:Autonomic Denervation; D001345:Autoradiography; D001921:Brain / Q000098:blood supply / Q000378:metabolism*; D002540:Cerebral Cortex / Q000098:blood supply / Q000378:metabolism; D002560:Cerebrovascular Circulation; D006624:Hippocampus / Q000098:blood supply; D000922:Immunotoxins / Q000494:pharmacology; D007457:Iodine Radioisotopes / Q000378:metabolism; D008297:Male; D009699:N-Glycosyl Hydrolases; D010940:Plant Proteins / Q000494:pharmacology; D016548:Prosencephalon / Q000502:physiology; D011813:Quinuclidinyl Benzilate / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000378:metabolism*; D012039:Regional Blood Flow; D054789:Ribosome Inactivating Proteins, Type 1; D000076985:Saporins; D019690:Technetium Tc 99m Exametazime / Q000378:metabolism
D000922:Immunotoxins; D007457:Iodine Radioisotopes; D010940:Plant Proteins; D011976:Receptors, Muscarinic; D054789:Ribosome Inactivating Proteins, Type 1; D019690:Technetium Tc 99m Exametazime; D011813:Quinuclidinyl Benzilate; C040062:3-quinuclidinyl-4-iodobenzilate; D000110:Acetylcholinesterase; D009699:N-Glycosyl Hydrolases; D000076985:Saporins; D000109:Acetylcholine