Differentially expressed genes in activin-induced apoptotic LNCaP cells.
Gene transcripts differentially expressed in activin-induced human prostatic LNCaP apoptotic cells have been discovered by an improved subtractive hybridization method, uracil-DNA subtraction assay (USA), which involves digestion with uracil-DNA glycosylase and mung-bean nuclease. Among the five up-regulated and seven down-regulated genes, we have identified six known (>95% homology and similar size; p16, p53, Siva, RHAMM, Pax2, and eIF-4a1), three homologues (>95% homology but different size; myosin, a helicase motif, and a kinase motif), and three novel genes (no homology). In addition, anti-sense knock-out of a resulting novel kinase-like gene was found to abolish the apoptotic DNA fragmentation in activin-treated LNCaP cells. These findings indicate a new potential mechanism in DNA fragmentation of activin-induced cell-cycle arresting and apoptosis.
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Epiclone Inc., 731 S. Chapel Avenue, Suite F, Alhambra, California, 91801, USA.
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D028341:Activins; D016375:Antisense Elements (Genetics) / Q000235:genetics; D017209:Apoptosis; D015152:Blotting, Northern; D003001:Cloning, Molecular; D053938:DNA Fragmentation / Q000187:drug effects; D045647:DNA Glycosylases; D015536:Down-Regulation / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects*; D015723:Gene Library; D005796:Genes; D006801:Humans; D007265:Inhibins / Q000494:pharmacology*; D008297:Male; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010770:Phosphotransferases / Q000235:genetics / Q000502:physiology; D011467:Prostate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000378:metabolism; D014162:Transfection; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000187:drug effects; D051981:Uracil-DNA Glycosidase
D016375:Antisense Elements (Genetics); D012333:RNA, Messenger; D028341:Activins; D007265:Inhibins; D010770:Phosphotransferases; D015719:Single-Strand Specific DNA and RNA Endonucleases; D045647:DNA Glycosylases; D009699:N-Glycosyl Hydrolases; D051981:Uracil-DNA Glycosidase