Characterization of mdcR, a regulatory gene of the malonate catabolic system in Klebsiella pneumoniae.
The Klebsiella pneumoniae mdcR gene, which encodes a LysR-type regulator, was overexpressed in Escherichia coli. Purified MdcR was found to bind specifically to the control region of either the malonate decarboxylase (mdc) genes or mdcR. We have also demonstrated that MdcR is an activator of the expression of the mdc genes, whereas it represses the transcription of the putative control region of mdcR, PmdcR, indicating a negative autoregulatory control.
Peng
H L
HL
Institute of Biological Science and Technology, National Chiao Tung University, Hsin Chu, Taiwan, Republic of China.
Shiou
S R
SR
Chang
H Y
HY
D000595:Amino Acid Sequence; D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial; D004926:Escherichia coli; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008314:Malonates / Q000378:metabolism*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification
D004269:DNA, Bacterial; D008314:Malonates; D011993:Recombinant Fusion Proteins; D002262:Carboxy-Lyases; C071852:malonate decarboxylase