title: Ammonium ion and glucosamine dependent increases of oligosaccharide complexity in recombinant glycoproteins secreted from cultivated BHK-21 cells.
abstract: The effect of different ammonium concentrations and glucosamine on baby hamster kidney (BHK)-21 cell cultures grown in continuously perfused double membrane bioreactors was investigated with respect to the final carbohydrate structures of a secretory recombinant glycoprotein. The human interleukin-2 (IL-2) mutant glycoprotein variant IL-Mu6, which bears a novel N-glycosylation site (created by a single amino acid exchange of Gln100 to Asn), was produced under different defined protein-free culture conditions in the presence or absence of either glutamine, NH4Cl, or glucosamine. Recombinant glycoprotein products were purified and characterized by amino acid sequencing and carbohydrate structural analysis using matrix-assisted laser desorption ionization time of flight mass spectrometry, high-pH anion-exchange chromatography with pulsed amperometric detection, and methylation analysis. In the absence of glutamine, cells secreted glycoprotein forms with preponderantly biantennary, proximal fucosylated carbohydrate chains (85%) with a higher NeuAc content (58%). Under standard conditions in the presence of 7.5 mM glutamine, complex-type N-glycans were found to be mainly biantennary (68%) and triantennary structures (33%) with about 50% containing proximal alpha1-6-linked fucose; 37% of the antenna were found to be substituted with terminal alpha2-3-linked N-acetylneuraminic acid. In the presence of 15 mM exogenously added NH4Cl, a significant and reproducible increase in tri- and tetraantennary oligosaccharides (45% of total) was detected in the secretion product. In glutamin-free cultures supplemented with glucosamine, an intermediate amount of high antennary glycans was detected. The increase in complexity of N-linked oligosaccharides is considered to be brought about by the increased levels of intracellular uridine diphosphate-GlcNAc/GalNAc. These nucleotide sugar pools were found to be significantly elevated in the presence of high NH3/NH4+ and glucosamine concentrations.
authors: Cell Culture Technology Department, GBF-Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany.gawlitzek.martin@gene.com
authors: M
authors: M
authors: Gawlitzek
authors: U
authors: U
authors: Valley
authors: R
authors: R
authors: Wagner
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis / Q000378:metabolism; D000117:Acetylglucosamine / Q000032:analysis / Q000378:metabolism; D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002478:Cells, Cultured / Q000187:drug effects; D002852:Chromatography, Ion Exchange / Q000379:methods; D006224:Cricetinae; D005944:Glucosamine / Q000378:metabolism* / Q000494:pharmacology; D006023:Glycoproteins / Q000737:chemistry* / Q000378:metabolism*; D006031:Glycosylation; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000166:cytology; D008745:Methylation; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000378:metabolism* / Q000494:pharmacology; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014530:Uridine Diphosphate / Q000032:analysis / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D007376:Interleukin-2; D009844:Oligosaccharides; D000644:Quaternary Ammonium Compounds; D011994:Recombinant Proteins; D014530:Uridine Diphosphate; D000116:Acetylgalactosamine; D005944:Glucosamine; D000117:Acetylglucosamine