cAMP-dependent induction of PDE5 expression in murine neuroblastoma cell differentiation.
The present study demonstrates, in both hybrid NG108-15 and mouse neuroblastoma N18TG2 cells, the presence and regulation of PDE5 mRNA during cell differentiation. PDE5 cDNA probes in Northern blot analysis recognize a approximately 9 kb transcript in bovine lung as well as in mouse neuroblastoma cells. Hybridization on total RNA extracted from dibutyryl-cAMP-treated NG108-15 cells shows a 5-fold increase of PDE5 9 kb mRNA: such an increase is not observed in N18TG2 although we observed a similar increase in the enzymatic activity of both cell lines. Our data demonstrate that PDE5 gene expression can be regulated by cAMP and suggest the existence of a complex regulatory system for PDE5 activity.
Giordano
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Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma La Sapienza, Italy.
Giorgi
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M
Sette
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C
Biagioni
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S
Augusti-Tocco
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G
D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000096:biosynthesis* / Q000235:genetics; D000818:Animals; D003994:Bucladesine / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism*; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; D015342:DNA Probes; D018076:DNA, Complementary; D004790:Enzyme Induction; D015870:Gene Expression; D008168:Lung / Q000201:enzymology / Q000473:pathology; D051379:Mice; D009447:Neuroblastoma; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
D015342:DNA Probes; D018076:DNA, Complementary; D012333:RNA, Messenger; D003994:Bucladesine; D000242:Cyclic AMP; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D054706:Cyclic Nucleotide Phosphodiesterases, Type 5; C517382:Pde5a protein, mouse