Neural cell surface differentiation antigen gp130(RB13-6) induces fibroblasts and glioma cells to express astroglial proteins and invasive properties.
Transient expression of the differentiation and tumor cell surface antigen gp130(RB13-6) characterizes a subset of rat glial progenitor cells susceptible to ethylnitrosourea-induced neurooncogenesis. gp130(RB13-6) is as a member of an emerging protein family of ecto-phosphodiesterases/nucleotide pyrophosphatases that includes PC-1 and the tumor cell motility factor autotaxin. We have investigated the potential role of gp130(RB13-6) in glial differentiation by transfection of three cell lines of different origin that do not express endogenous gp130(RB13-6) (NIH-3T3 mouse fibroblasts; C6 and BT7Ca rat glioma cells) with the cDNA encoding gp130(RB13-6). The effect of gp130(RB13-6) expression was analyzed in terms of overall cell morphology, the expression of glial cell-specific marker proteins, and invasiveness. Transfectant sublines, consisting of 100% gp130(RB13-6)-positive cells, exhibited an altered, bipolar morphology. Fascicular aggregates of fibroblastoid cells subsequently developed into mesh-like patterns. Contrary to the parental NIH-3T3 and BT7Ca cells, the transfectant cells invaded into collagen type I. As shown by immunofluorescence staining of the transfectant sublines as well as of primary cultures composed of gp130(RB13-6)-positive and -negative cells, expression of gp130(RB13-6) induced coexpression of proteins typical for glial cells and their precursors, i.e., glial fibrillary acidic protein, the low affinity nerve growth factor receptor, and the neural proteins Thy-1, Ran-2, and S-100. In accordance with its expression in the immature rat nervous system, gp130(RB13-6) may thus have a significant role in the glial differentiation program and its subversion in neurooncogenesis.
Deissler
H
H
Institute of Cell Biology (Cancer Research), University of Essen Medical School, D-45122 Essen, Germany.
Blass-Kampmann
S
S
Bruyneel
E
E
Mareel
M
M
Rajewsky
M F
MF
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D003094:Collagen / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D005910:Glioma / Q000378:metabolism / Q000473:pathology*; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics; D009361:Neoplasm Invasiveness; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis; D012583:Schwann Cells / Q000378:metabolism / Q000473:pathology; D052118:Lysosomal-Associated Membrane Protein 1
D000943:Antigens, Differentiation; D000951:Antigens, Neoplasm; D003094:Collagen; D051907:Lysosomal Membrane Proteins; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; C489117:Lamp1 protein, rat; D052118:Lysosomal-Associated Membrane Protein 1