title: Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes.
abstract: We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes loss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.
authors: Departamento de Biología, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
authors: S
authors: S
authors: Hernández-Allés
authors: V J
authors: VJ
authors: Benedí
authors: L
authors: L
authors: Martínez-Martínez
authors: A
authors: A
authors: Pascual
authors: A
authors: A
authors: Aguilar
authors: J M
authors: JM
authors: Tomás
authors: S
authors: S
authors: Albertí
mesh_terms: D001426:Bacterial Proteins; D002440:Cefoxitin / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D018272:Porins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D002513:Cephamycins; D004251:DNA Transposable Elements; C092233:OmpK36 protein, Klebsiella pneumoniae; D018272:Porins; D002440:Cefoxitin