title: Modelling of oligosaccharide synthesis by dextransucrase.
abstract: Dextransucrase catalyses the formation of dextran, but also of numerous oligosaccharides from sucrose and different acceptors, if appropriate conditions are chosen. Much experimental work has been carried out and a scheme of reactions and a mathematical model have been developed to describe the complex kinetic behaviour of the enzyme. A computer program was used to calculate the parameters of the model from a broad range of experimental data, investigating a large number of kinetic tests with the acceptors maltose and fructose. The results lead to design considerations for a continuous reactor system with immobilized dextransucrase to produce leucrose, a disaccharide of industrial interest.
authors: Lehrstuhl für Technologie der Kohlenhydrate, Technical University of Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany.
authors: B
authors: B
authors: Demuth
authors: H J
authors: HJ
authors: Jördening
authors: K
authors: K
authors: Buchholz
mesh_terms: D019149:Bioreactors; D001709:Biotechnology; D004187:Disaccharides / Q000096:biosynthesis; D004800:Enzymes, Immobilized; D005632:Fructose / Q000378:metabolism; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology; D008320:Maltose / Q000378:metabolism; D008954:Models, Biological; D009844:Oligosaccharides / Q000096:biosynthesis*; D012680:Sensitivity and Specificity; D013395:Sucrose / Q000378:metabolism
chemical_list: D004187:Disaccharides; D004800:Enzymes, Immobilized; D009844:Oligosaccharides; D005632:Fructose; D013395:Sucrose; D008320:Maltose; D005964:Glucosyltransferases; C020025:dextransucrase; C060525:leucrose