title: Mutations that confer resistance to 2-deoxyglucose reduce the specific activity of hexokinase from Myxococcus xanthus.
abstract: The glucose analog 2-deoxyglucose (2dGlc) inhibits the growth and multicellular development of Myxococcus xanthus. Mutants of M. xanthus resistant to 2dGlc, designated hex mutants, arise at a low spontaneous frequency. Expression of the Escherichia coli glk (glucokinase) gene in M. xanthus hex mutants restores 2dGlc sensitivity, suggesting that these mutants arise upon the loss of a soluble hexokinase function that phosphorylates 2dGlc to form the toxic intermediate, 2-deoxyglucose-6-phosphate. Enzyme assays of M. xanthus extracts reveal a soluble hexokinase (ATP:D-hexose-6-phosphotransferase; EC 2.7.1.1) activity but no phosphotransferase system activities. The hex mutants have lower levels of hexokinase activities than the wild type, and the levels of hexokinase activity exhibited by the hex mutants are inversely correlated with the ability of 2dGlc to inhibit their growth and sporulation. Both 2dGlc and N-acetylglucosamine act as inhibitors of glucose turnover by the M. xanthus hexokinase in vitro, consistent with the finding that glucose and N-acetylglucosamine can antagonize the toxic effects of 2dGlc in vivo.
authors: Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052, USA. pay@uidaho.edu
authors: P
authors: P
authors: Youderian
authors: M C
authors: MC
authors: Lawes
authors: C
authors: C
authors: Creighton
authors: J C
authors: JC
authors: Cook
authors: M H
authors: MH
authors: Saier
mesh_terms: D000117:Acetylglucosamine; D003847:Deoxyglucose / Q000378:metabolism* / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005941:Glucokinase / Q000235:genetics; D005944:Glucosamine / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006593:Hexokinase / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis; D016296:Mutagenesis; D016941:Myxococcus xanthus / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D013171:Spores, Bacterial
chemical_list: D003847:Deoxyglucose; D006593:Hexokinase; D005941:Glucokinase; D005944:Glucosamine; D005990:Glycerol; D000117:Acetylglucosamine