Effect of ecological factors on the inhibitory spectrum and activity of bacteriocins.
The effect of food components and ecological factors on the activities of nisin, sakacin P and curvacin A was evaluated. Lactobacillus curvatus, Listeria innocua, Salmonella and Escherichia coli including E. coli O157:H7 were used as target organisms. Lecithin, casein, and divalent cations were antagonists of the bacteriocins at 0.1%, 0.1% and 10 mmol l(-1), respectively. A decrease in pH as well as the presence of EDTA, propyl-parabene or NaCl at concentrations of 0-1 mmol y(-1), 0-0.16 g l(-1), and 0-6% (w/w), respectively, increased the activity of all bacteriocins. These compounds as well as a pH < 5.5 rendered the Gram-negative target organisms sensitive against bacteriocins. Of practical importance is the respective effect of NaCl at concentrations > 5% which are achieved in fermentation and ripening processes, e.g. in production of fermented sausages. A characteristic response was observed for each of the bacteriocins. It is suggested that bacteriocins of lactic acid bacteria are effective against a wide range of microorganisms including E. coli O157:H7 if applied in combination with other preservative principles prevailing in foods.
Gänzle
M G
MG
Universität Hohenheim, Institut für Legensmitteltechnologie, Stuttgart, Germany.
Weber
S
S
Hammes
W P
WP
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001430:Bacteriocins / Q000494:pharmacology*; D003470:Culture Media; D004492:Edetic Acid / Q000494:pharmacology; D019453:Escherichia coli O157 / Q000187:drug effects / Q000254:growth & development; D005516:Food Microbiology; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008087:Listeria / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D009561:Nisin / Q000494:pharmacology; D010226:Parabens / Q000494:pharmacology; D012475:Salmonella / Q000187:drug effects / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D003470:Culture Media; D010226:Parabens; C071456:sakacin A; D009561:Nisin; C087217:sakacin P protein, Lactobacillus; D012965:Sodium Chloride; D004492:Edetic Acid; C038193:4-hydroxybenzoic acid; C006068:propylparaben