Role of dNTPs in mutagenesis.
The induced mutation frequency by alkylating mutagen glycidyl methacrylate (GMA) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was investigated with or without perturbation of deoxyribonucleoside triphosphate (dNTP) pools; the influence of short treatment at different concentrations of GMA or MNNG on dNTP pools was also explored. The results indicated that the induced mutation frequency increased greatly at high dosages of mutagen (GMA approximately 64 micrograms/ml, MNNG approximately 8 micrograms/ml) and the perturbation on dNTP pools was carried out before the treatment of mutagen; the short treatment with mutagen could induce distinct fluctuations of dNTP pools, but different mutagen might have different effects on dNTP pools. According to the results of the present study and other reports in literature, we conclude that dNTP pools may be the targets of alkylating mutagens and the fluctuations of dNTP pools are closely associated with mutagenesis.
Lei
H X
HX
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS), Beijing, China.
Li
Z S
ZS
Xie
D Y
DY
Liu
B C
BC
Zuo
J
J
Fang
F D
FD
D016475:3T3 Cells; D000818:Animals; D003854:Deoxyribonucleotides / Q000502:physiology*; D004852:Epoxy Compounds / Q000494:pharmacology*; D008689:Methacrylates / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D016296:Mutagenesis / Q000502:physiology*; D009153:Mutagens / Q000494:pharmacology*; D018675:Toxicity Tests
D003854:Deoxyribonucleotides; D004852:Epoxy Compounds; D008689:Methacrylates; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; C007870:glycidyl methacrylate