Vaccination with experimental feline immunodeficiency virus vaccines, based on autologous infected cells, elicits enhancement of homologous challenge infection.
Cats were vaccinated with fixed autologous feline immunodeficiency virus (FIV)-infected cells in order to present viral proteins to the immune system of individual cats in an MHC-matched fashion. Upon vaccination, a humoral response against Gag was induced. Furthermore, virus-neutralizing antibodies were detected in a Crandell feline kidney cell-based neutralization assay, but not in a neutralization assay based on primary peripheral blood mononuclear cells. Despite the induction of these FIV-specific responses, vaccinated cats were not protected. Instead, accelerated virus replication was found, an observation similar to what previous experiments using other vaccine candidates have shown. Here, the results of the present study are discussed in the light of enhancement of lentivirus infections as a complicating factor in lentivirus vaccine development.
Karlas
J A
JA
Siebelink
K H
KH
Peer
M A
MA
Huisman
W
W
Cuisinier
A M
AM
Rimmelzwaan
G F
GF
Osterhaus
A D
AD
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000097:blood; D002415:Cats; D002478:Cells, Cultured; D018920:Coculture Techniques; D004195:Disease Models, Animal; D005557:Formaldehyde; D015683:Gene Products, gag / Q000097:blood / Q000276:immunology; D007111:Immunity, Cellular; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development* / Q000276:immunology*; D007700:Kinetics; D016180:Lentivirus Infections / Q000097:blood / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology / Q000821:virology; D008285:Major Histocompatibility Complex / Q000276:immunology; D009500:Neutralization Tests; D011108:Polymers; D011498:Protein Precursors / Q000097:blood / Q000276:immunology; D016707:Tissue Fixation; D014611:Vaccination; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects / Q000737:chemistry / Q000276:immunology; D019562:Viral Load; D014764:Viral Proteins; D014765:Viral Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000737:chemistry / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; C069338:FIV gag precursor protein, Feline immunodeficiency virus; D015683:Gene Products, gag; D011108:Polymers; D011498:Protein Precursors; D015164:Vaccines, Inactivated; D014764:Viral Proteins; D014765:Viral Vaccines; D005557:Formaldehyde; C003043:paraform