title: Tubular morphogenesis and mesenchymal interactions affect renin expression and secretion in SIMS mouse submandibular cells.
abstract: We have previously immortalized a mouse submandibular gland (SMG) ductal epithelial cell line, SIMS, from pubertal male mice transgenic for the SV40 large T antigen under the control of the adenovirus 5 E1A promoter. Here we demonstrate the role of the extracellular environment in directing not only the morphogenetic behavior of the cells, but also their functional differentiation in terms of renin expression and secretion. First, we measured renin activity of polarized SIMS cells. Low levels of renin are secreted from both the apical and the basolateral domains; the mechanism appears to be direct as no renin was found to be transcytosed across the cell. Second, we studied homotypic and heterotypic mesenchymal cell interactions with SIMS cells. We found that epithelial-mesenchymal coculture in collagen I gels results in branching tubular morphogenesis of SIMS cells and that significant amounts of renin are secreted, probably into the lumen, as the precursor form, prorenin. Third, we investigated the effects of the basement membrane on SIMS cell morphology and function and found that this structure alone is sufficient to allow expression and secretion of both prorenin and active renin. Finally, we established that SIMS cells can express androgen-regulated genes in a transient transfection assay. In addition, in Matrigel cultures androgen receptor expression appears to be induced, suggesting that the SIMS cell line will be useful for further studies on the molecular basis of the observed high-level expression of SMG-specific genes in male mice.
authors: Unité de Génétique et Biochimie du Développement, URA CNRS 1960, Institut Pasteur, 25, rue du Dr. Roux, Paris Cédex 15, 75724, France. blaoide@pasteur.fr
authors: B M
authors: BM
authors: Laoide
authors: I
authors: I
authors: Gastinne
authors: F
authors: F
authors: Rougeon
mesh_terms: D000818:Animals; D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D002454:Cell Differentiation; D002455:Cell Division; D002460:Cell Line; D003094:Collagen; D005109:Extracellular Matrix; D005260:Female; D005782:Gels; D005786:Gene Expression Regulation; D008297:Male; D008648:Mesoderm; D051379:Mice; D008811:Mice, Inbred DBA; D008822:Mice, Transgenic; D009024:Morphogenesis; D011944:Receptors, Androgen; D012083:Renin / Q000378:metabolism*; D013363:Submandibular Gland / Q000166:cytology
chemical_list: D000952:Antigens, Polyomavirus Transforming; D005782:Gels; D011944:Receptors, Androgen; D003094:Collagen; D012083:Renin