title: Studies on a haemolymph lectin isolated from Rhodnius prolixus and its interaction with Trypanosoma rangeli.
abstract: We demonstrated that in Rhodnius prolixus haemocyte monolayers, both Trypanosoma cruzi and Trypanosoma rangeli are capable of inducing haemocyte/parasite clump formation. We also purified, by one-step affinity chromatography, a haemolymph galactoside-binding lectin from R. prolixus which we believe could play an important role in the development of T. rangeli in the haemocoel of the insect vector. This lectin markedly enhanced the activation of clump formation by T. rangeli in R. prolixus haemocyte monolayers, with an increase in clump size and haemocyte aggregation. The haemolymph lectin also significantly affected the motilitity and survival of T. rangeli culture short forms, but not the long forms, when they were incubated in vitro. This molecule is also one of the few described in insects with agglutination activity independent of calcium ions. The partial N-terminal amino acid sequence of this lectin demonstrated similarity to a bacterial xylulose kinase and in preliminary experiments the purified haemolymph lectin phosphorylated a tyrosine kinase substrate in a dose-dependent manner. The possible role of this haemolymph lectin in the life cycle of T. rangeli is discussed.
authors: Department of General Biology, Universidade Federal Fluminense, Niteroi, RJ, CEP 24.001-970, Brazil.
authors: C B
authors: CB
authors: Mello
authors: Y
authors: Y
authors: Nigam
authors: E S
authors: ES
authors: Garcia
authors: P
authors: P
authors: Azambuja
authors: R P
authors: RP
authors: Newton
authors: N A
authors: NA
authors: Ratcliffe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects; D006434:Hemocytes / Q000378:metabolism / Q000469:parasitology; D006458:Hemolymph / Q000737:chemistry / Q000469:parasitology*; D007303:Insect Vectors / Q000469:parasitology*; D037102:Lectins / Q000097:blood / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D008969:Molecular Sequence Data; D009068:Movement / Q000187:drug effects; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000737:chemistry / Q000378:metabolism; D012239:Rhodnius / Q000469:parasitology*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D014345:Trypanosoma / Q000187:drug effects / Q000502:physiology*
chemical_list: D006023:Glycoproteins; D037102:Lectins; D017853:Phosphotransferases (Alcohol Group Acceptor); C032700:xylulokinase