title: Histology and tissue chemistry of tidemark separation in hamsters.
abstract: Adult articular cartilage is divided by the tidemark into a deep calcified layer and a more superficial uncalcified layer. Histologic examination of articular cartilage from the knee joint of golden Syrian hamsters 123 days of age or older revealed defects at the tidemark in the tibia. Defects ranged from small separations of the calcified and uncalcified layers along the tidemark to progressively larger defects apparently formed by dissolution. These larger defects appeared as cavities in the noncalcified cartilage, had smooth rather than rough edges, frequently contained coalesced debris, and often resulted in a bulge in the articular surface. Occasionally, these large defects broke through the articular surface. Defects were not observed in tibial cartilage of younger (<90 days old) hamsters or in femoral cartilage from hamsters of any age. Exercise neither protected against nor increased the severity of the defects. Collagen cross-linking by pyridinoline was examined as a function of age and increased from 1,090 to 3,062 micromoles of pyridinoline/mole of hydroxyproline over the period of 1-9 months of age but was not correlated with defect formation. With increasing age, these focal tidemark defects could lead to osteoarthrosis-like cartilage lesions.
authors: Department of Cancer, Immunology and Infectious Diseases, Central Research Division, Pfizer, Groton, CT 06340, USA. otteri@pfizer.com
authors: I G
authors: IG
authors: Otterness
authors: M
authors: M
authors: Chang
authors: J E
authors: JE
authors: Burkhardt
authors: F J
authors: FJ
authors: Sweeney
authors: A J
authors: AJ
authors: Milici
mesh_terms: D000367:Age Factors; D000818:Animals; D002114:Calcinosis / Q000473:pathology / Q000662:veterinary*; D002358:Cartilage, Articular / Q000473:pathology* / Q000502:physiology; D002853:Chromatography, Liquid / Q000662:veterinary; D003094:Collagen / Q000737:chemistry; D006224:Cricetinae; D004801:Eosine Yellowish-(YS) / Q000737:chemistry; D005260:Female; D006416:Hematoxylin / Q000737:chemistry; D006614:Hindlimb; D006909:Hydroxyproline / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D007596:Joints / Q000473:pathology*; D008647:Mesocricetus / Q000033:anatomy & histology* / Q000502:physiology; D010003:Osteoarthritis / Q000473:pathology / Q000662:veterinary*; D010619:Phenazines / Q000737:chemistry; D010805:Physical Conditioning, Animal; D012376:Rodent Diseases / Q000473:pathology*
chemical_list: D007202:Indicators and Reagents; D010619:Phenazines; D003094:Collagen; D006909:Hydroxyproline; D004801:Eosine Yellowish-(YS); C009195:safranine T; D006416:Hematoxylin