Effect of Spumol K on the ultrastructure of Aspergillus niger strains sensitive or resistant to toxic compounds of beet molasses.
Aspergillus niger strains sensitive and resistant to toxic compounds of beet molasses grown in the presence of Spumol K were the object of the present studies. The antifoamer used diminished the number of mitochondria in both groups of strains and caused the reduction of their cristae in the sensitive ones. The disturbances in the ultrastructure of nucleus and mitochondria appeared mostly in sensitive strains. On the other hand, Spumol K presence in resistant strains not only increased the number of deposits of electron dense material in mitochondria, vacuoles, cytoplasm and in nuclear envelope but also was responsible for multivesicular body formation. The differences also existed in the ultrastructure of the cell wall: in the sensitive strains treated with Spumol K, the cell wall was completely deprived of fibrillar component, only granular component was present. In the resistant strains, granular component dominated, although fibrillar one was still visible. The present studies suggest that antifoamer in resistant strains becomes inactivated in different cell compartments, therefore its toxicity is lower than in sensitive strains where defence mechanism, inactivating toxic substances, is weaker and toxicity stronger.
Gabara
B
B
Department of Plant Cytology and Cytochemistry, University of Lódź, Poland.
Zakowska
Z
Z
D000934:Antifoaming Agents / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects* / Q000235:genetics / Q000648:ultrastructure; D002467:Cell Nucleus / Q000187:drug effects; D002473:Cell Wall / Q000187:drug effects; D004351:Drug Resistance / Q000235:genetics; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects; D008965:Molasses / Q000633:toxicity
D000934:Antifoaming Agents