The effect of hydrogen peroxide upon beta-adrenoceptor density and function in C6 rat glioma cells.
Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer's disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of beta-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface beta 1, or beta 2-adrenoceptors, measured using the hydrophilic ligand [3H](-)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to Gs stimulation found in post-mortem brain samples from Alzheimer's disease autopsy cases is unlikely to be mediated by hydrogen peroxide.
Bergström
D
D
Department of Pharmacology, Umeå University, Sweden.
Jacobsson
S O
SO
Fowler
C J
CJ
D000262:Adenylyl Cyclases / Q000378:metabolism; D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D005576:Colforsin / Q000494:pharmacology; D016895:Culture Media, Serum-Free; D000242:Cyclic AMP / Q000378:metabolism; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000502:physiology; D005910:Glioma / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D011412:Propanolamines / Q000378:metabolism; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects* / Q000502:physiology*; D014407:Tumor Cells, Cultured
D000318:Adrenergic beta-Agonists; D016895:Culture Media, Serum-Free; D011412:Propanolamines; D011943:Receptors, Adrenergic, beta; D005576:Colforsin; D006861:Hydrogen Peroxide; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase; D019205:GTP-Binding Protein alpha Subunits, Gs; D000262:Adenylyl Cyclases; D007545:Isoproterenol; C033394:CGP 12177