High-resolution deletion mapping of chromosome arm 1p in pancreatic cancer identifies a major consensus region at 1p35.
Chromosomal arm 1p has long been suspected, on the basis of loss of heterozygosity (LOH) and other data, to harbor a tumor suppressor gene important in pancreatic carcinomas and other tumors. We constructed a high-resolution map of LOH at I p in a panel of pancreatic adenocarcinomas. Using 44 markers, we identified LOH on I p in 49% of 43 cancers. Breakpoints in 1p were identified in 15 of the carcinomas and could be used to ascertain consensus patterns. We found a major consensus region of LOH at 1p35 between loci D1S233 and D1S247. This region participates in the majority of LOH events on 1 p in pancreatic cancer. These data provide a roadmap for further regional mapping, homozygous deletion searches, comparison to LOH patterns seen in other tumor types, and prioritization of studies using candidate genes.
Hilgers
W
W
Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.
Tang
D J
DJ
Sugar
A Y
AY
Shekher
M C
MC
Hruban
R H
RH
Kern
S E
SE
D000230:Adenocarcinoma / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics*; D016384:Consensus Sequence; D006801:Humans; D019656:Loss of Heterozygosity / Q000235:genetics; D051379:Mice; D008819:Mice, Nude; D010190:Pancreatic Neoplasms / Q000235:genetics*; D014183:Transplantation, Heterologous