title: Interleukin-6 levels in co-culture of human in vitro fertilization embryos with Vero cells are not predictive of future successful development.
abstract: OBJECTIVE
  In an attempt to predict successful embryo transfer and implantation, we measured interleukin (IL)-6 levels in culture supernatants of co-cultured preimplantation human embryos. We tested whether all in vitro fertilized human embryos in co-cultures do secrete IL-6, and whether there was any difference in such production between embryos that successfully reached the blastocyst stage and blocked embryos. We also addressed the question of IL-6 secretion by co-culture support cells, namely Vero cells themselves.
  
  
  METHODS
  Each fertilized oocyte was cultured individually and transferred in culture wells supplemented with a feeder layer of Vero cells at day 2. In vitro IL-6 production was measured by bioassay of the culture media.
  
  
  RESULTS
  Because Vero cells themselves secrete IL-6, it became impossible, in co-culture, to quantify production of IL-6 by the sole embryos. On the other hand, the co-culture technique has shown us that embryos are likely to consume IL-6. There was no difference between blastocysts and blocked embryos.
  
  
  CONCLUSIONS
  IL-6 levels in human embryo co-cultures do not correlate with future successful embryo transfer.
authors: INSERM U131, Equipe Cytokines dans la relation materno-foetale, Bâtiment de Gynécologie Obstétrique, Hôpital Antoine Béclère, Clamart, France.
authors: G
authors: G
authors: Laprée-Delage
authors: M
authors: M
authors: Volante
authors: R
authors: R
authors: Frydman
authors: G
authors: G
authors: Chaouat
mesh_terms: D000328:Adult; D000818:Animals; D001755:Blastocyst / Q000378:metabolism / Q000502:physiology*; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D003470:Culture Media; D004624:Embryo Transfer; D005314:Embryonic and Fetal Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D011237:Predictive Value of Tests; D014709:Vero Cells
chemical_list: D003470:Culture Media; D015850:Interleukin-6