Application of an in situ PCR hybridization method to detection of human T-lymphotropic virus type I-infected cells in the lung.
We applied an in situ polymerase chain reaction (PCR) hybridization method in order to detect human T-lymphotropic virus type I-infected cells in routinely-processed paraffin sections of the lung from 13 autopsied patients with adult T-cell leukemia (ATL). Previously reported protocol resulted in somewhat non-specific staining in our sections. Therefore, we used a hot start PCR method using specialized commercially-available polymerase in order to increase the specificity. Of 6 patients with ATL cell invasion into the lungs, 4 exhibited strong positive staining of almost all invading ATL cells. In contrast, 7 patients without ATL cell invasion into the lungs did not demonstrate any significant reactivity. Since the method described here is a relatively simple hot start method and does not yield false-positives, it may allow us to determine whether human T-lymphotropic virus type I (HTLV-I) associated disorders are related to lymphocytes integrating the HTLV-I genome.
Qiu
Y
Y
Department of Pathology, Kochi Medical School, Nankoku, Japan.
Takeuchi
T
T
Chen
B K
BK
Ohtsuki
Y
Y
D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000378:metabolism; D006800:Deltaretrovirus Infections / Q000235:genetics / Q000821:virology*; D005260:Female; D015368:Human T-lymphotropic virus 1 / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods*; D015458:Leukemia, T-Cell / Q000235:genetics / Q000473:pathology / Q000821:virology; D008171:Lung Diseases / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D011533:Proviruses / Q000235:genetics; D014018:Tissue Distribution / Q000502:physiology
D004279:DNA, Viral