title: Expression of tyrosine hydroxylase, calcitonin gene-related peptide, substance P and protein gene product 9.5 in mouse islets transplanted under the kidney capsule.
abstract: Pancreatic islets transplanted to the kidney of syngeneic mice were stained for calcitonin gene-related peptide (CGRP), substance P (SP), tyrosine hydroxylase (TH), acetylcholinesterase and the pan-neuronal marker, protein gene product 9.5 (PGP). Nerve fibers expressing TH-like immunoreactivity (TH-LI) and CGRP-LI were rare for 4 days but increased 2 (CGRP) or 6 (TH) weeks after transplantation. In 1-year-old grafts the CGRP-LI innervation resembled that in situ, while TH-LI and PGP-LI innervations were increased. SP-LI fibers remained rare throughout. Perikarya intrinsic to the islets did not show CGRP-LI or SP-LI. The results indicate a progressive ingrowth of sensory fibers into the grafts and that the TH-LI innervation becomes even more pronounced than in the pancreas. The post-transplantation reaction of islet intrinsic neurons does not involve CGRP and SP, contrasting with previous observations for vasoactive intestinal polypeptide.
authors: Department of Histology and Cell Biology, Umeå University, Sweden. Solveig.Persson-Sjogren@histocel.umu.se
authors: S
authors: S
authors: Persson-Sjögren
authors: S
authors: S
authors: Forsgren
authors: I B
authors: IB
authors: Täljedal
mesh_terms: D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis*; D005260:Female; D007150:Immunohistochemistry; D007515:Islets of Langerhans / Q000737:chemistry* / Q000294:innervation; D016381:Islets of Langerhans Transplantation; D007668:Kidney; D051379:Mice; D008810:Mice, Inbred C57BL; D009412:Nerve Fibers / Q000737:chemistry; D009474:Neurons / Q000737:chemistry; D013373:Substance P / Q000032:analysis*; D013869:Thiolester Hydrolases / Q000032:analysis*; D016042:Transplantation, Heterotopic; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis*; D043222:Ubiquitin Thiolesterase
chemical_list: D013373:Substance P; D014446:Tyrosine 3-Monooxygenase; D013869:Thiolester Hydrolases; D043222:Ubiquitin Thiolesterase; D015740:Calcitonin Gene-Related Peptide