An immunochemical focus assay to quantify replication competent and defective viruses involved in murine acquired immunodeficiency syndrome.
Murine acquired immunodeficiency syndrome is a lymphoproliferative disease with a concurrently developing immunodeficiency. The disease is induced after injection of supernatant of a chronically infected cell line that releases a mixture of two replication competent virus classes and a replication incompetent virus species responsible for pathogenicity. An immunochemical detection assay for virus infected foci on cell monolayers has been developed. This assay allows quantification of all three types of virus.
Hügin
A W
AW
Department of Dermatology, University Hospital, Geneva, Switzerland. ambros.hugin@hcuge.ch
Tang
Y
Y
D000818:Animals; D002460:Cell Line; D003673:Defective Viruses / Q000502:physiology*; D005260:Female; D007124:Immunoenzyme Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008908:Mink Cell Focus-Inducing Viruses / Q000502:physiology; D016183:Murine Acquired Immunodeficiency Syndrome / Q000821:virology*; D014779:Virus Replication