title: Induction and enhancement of stress proteins in a trichloroethylene-degrading methanotrophic bacterium, Methylocystis sp. M.
abstract: The responses of the trichloroethylene-degrading bacterium Methylocystis sp. M to six different water-pollutants, carbon starvation, and temperature-shock (heat and cold) were examined using 2-dimensional gel electrophoresis. Twenty-eight polypeptides were induced, and these stress-induced proteins were classified into three groups. Some of the chemically induced proteins were the same as those induced by carbon starvation and temperature-shock. Two of the polypeptides were induced by trichloroethylene. Trichloroethylene-stress protein synthesis required 1-2 h at a concentration of trichloroethylene that had no effect on growth. Furthermore, 25 stress-enhanced polypeptides were observed, and one of these was enhanced by trichloroethylene. Based on these results, we discuss applications of chemical-stress induction of proteins to establish effective bioremediation and bioassay by methanotrophs.
authors: National Institute for Environmental Studies, Ibaraki, Japan. huchiyam@nies.go.jp
authors: H
authors: H
authors: Uchiyama
authors: Y
authors: Y
authors: Shinohara
authors: N
authors: N
authors: Tomioka
authors: I
authors: I
authors: Kusakabe
mesh_terms: D001419:Bacteria / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D001673:Biodegradation, Environmental; D002244:Carbon / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D007700:Kinetics; D013696:Temperature; D014241:Trichloroethylene / Q000378:metabolism* / Q000494:pharmacology; D014874:Water Pollutants, Chemical / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D006360:Heat-Shock Proteins; D006843:Hydrocarbons, Chlorinated; D014874:Water Pollutants, Chemical; D014241:Trichloroethylene; D002244:Carbon