Age-dependent changes in rat liver microsomal membrane structure and functions under benzene treatment.
The influence of benzene on 3 and 24 months old rat liver microsomes was studied. Some structural and functional changes occur under benzene treatment in the cytochrome P-450 system which are more pronounced in 3 months old rat microsomes than in the 24 months ones. Glucose-6-phosphatase and glucose dehydrogenase activities indicate that 3 months old rat microsomal vesicles are more stable against benzene injury than those, of 24 months old ones. In vitro benzene hydroxylation activation by NADPH addition decreased disruptive xenobiotic's effect on 3 but not on 24 months old rat liver microsomal vesicles. This fact suggests that the rate of benzene hydroxylation is important for its membrane damaging action effect. Thus, age-related differences in xenobiotic action on liver microsomes could be related to the decrease of benzene metabolism rate with senescence.
Sukhodub
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AL
Research Institute of Dermatology and Venerology, Kharkiv, Ukraine. andrey.l.sukhodub@univer.kharkov.ua
Padalko
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D000375:Aging / Q000502:physiology*; D000818:Animals; D001554:Benzene / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D005500:Follow-Up Studies; D042843:Glucose 1-Dehydrogenase; D005948:Glucose Dehydrogenases / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008297:Male; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D003577:Cytochrome P-450 Enzyme System; D005948:Glucose Dehydrogenases; D042843:Glucose 1-Dehydrogenase; D005952:Glucose-6-Phosphatase; D001554:Benzene