Influence of polymolecular events on inactivation behavior of xylose isomerase from Thermotoga neapolitana 5068.
The inactivation behavior of the xylose isomerase from Thermotoga neapolitana (TN5068 XI) was examined for both the soluble and immobilized enzyme. Polymolecular events were involved in the deactivation of the soluble enzyme. Inactivation was biphasic at 95 degrees C, pH 7.0 and 7.9, the second phase was concentration-dependent. The enzyme was most stable at low enzyme concentrations, however, the second phase of inactivation was 3- to 30-fold slower than the initial phase. Both phases of inactivation were more rapid at pH 7.9, relative to 7.0. Differential scanning calorimetry of the TN5068 XI revealed two distinct thermal transitions at 99 degrees and 109 degrees C. The relative magnitude of the second transition was dramatically reduced at pH 7.9 relative to pH 7.0. Approximately 24% and 11% activity were recoverable after the first transition at pH 7.0 and 7.9, respectively. When the TN5068 XI was immobilized by covalent attachment to glass beads, inactivation was monophasic with a rate corresponding to the initial phase of inactivation for the soluble enzyme. The immobilized enzyme inactivation rate corresponded closely to the rate of ammonia release, presumably from deamidation of labile asparagine and/or glutamine residues. A second, slower inactivation phase suggests the presence of an unfolding intermediate, which was not observed for the immobilized enzyme. The concentration dependence of the second phase of inactivation suggests that polymolecular events were involved. Formation of a reversible polymolecular aggregate capable of protecting the soluble enzyme from irreversible deactivation appears to be responsible for the second phase of inactivation seen for the soluble enzyme. Whether this characteristic is common to other hyperthermophilic enzymes remains to be seen.
Hess
J M
JM
Department of Chemical Engineering, North Carolina State University, Box 7905, Stinson Road, Raleigh, North Carolina 27695-7905, USA.
Kelly
R M
RM
D019747:Aldose-Ketose Isomerases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001709:Biotechnology; D002152:Calorimetry, Differential Scanning; D004800:Enzymes, Immobilized; D016965:Gram-Negative Anaerobic Straight, Curved, and Helical Rods / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D017510:Protein Folding; D012995:Solubility; D013816:Thermodynamics
D004800:Enzymes, Immobilized; D000641:Ammonia; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase