IgG transcytosis is accelerated but its pathway is not disrupted by Brefeldin-A.
To see the action of Brefeldin-A (Bref-A) to transcytosis of the immunoglobulin G (IgG), we have examined the early stage of endocytosis and intracellular transport of IgG and transferrin (Tf) in epithelial cells of the rat yolk sac in culture with and without Bref-A. In the absence of Bref-A, the endocytosed IgG and Tf appeared in large apical endosomes during the first 5-15 min. At 15 min Tf-recycling endosomes and IgG-transferring vesicles also appeared in the apical or basolateral cytoplasm, respectively. At 15 min in the presence of Bref-A, clouds of Tf-recycling endosomes accumulated in the apical cytoplasm, while distinct IgG-transferring vesicles were distributed further to the basolateral cytoplasm. This differential action of Bref-A to IgG-transferring vesicles and Tf-recycling vesicles suggested an unexplored mechanism of sorting and basolateral transport of IgG.
Ichimura
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School of Nursing, Yamaguchi Prefectural University, Japan. ichimura@kan1.yamaguchi-pu.ac.jp
D000818:Animals; D020126:Brefeldin A / Q000494:pharmacology*; D004705:Endocytosis / Q000187:drug effects*; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D007074:Immunoglobulin G / Q000378:metabolism*; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D014168:Transferrin / Q000378:metabolism; D015017:Yolk Sac / Q000378:metabolism / Q000648:ultrastructure
D007074:Immunoglobulin G; D011500:Protein Synthesis Inhibitors; D014168:Transferrin; D020126:Brefeldin A