Presence of bleomycin-detectable free iron in the alveolar system of preterm infants.
Chronic lung disease (CLD) is a major cause of long term morbidity in preterm infants. Reactive oxygen species (ROS) play an important role in the pathogenesis of CLD. We show that a high percentage (63 to 83%) of the investigated bronchoalveolar secretions (BAS) of neonates contain bleomycin-detectable free iron concentrations (0. 04-0.124 nmol/micrograms SC, median range). Beside the presence of redox-active iron several iron-binding proteins like transferrin, ferritin and lactoferrin were determined in BAS. Comparison of protein distribution within the first three days of life showed slight differences between the group of preterm infants who developed CLD and the neonates who recovered from RDS. Because of the existence of free iron we suggest a higher risk of hydroxyl radical formation in the alveolar space. In an artificial system with addition of iron and hydrogen peroxide we were able to demonstrate OH-radical production in BAS by electron paramagnetic resonance (EPR). OH-radical formation by H2O2 and iron in buffer solution was slightly enhanced in the presence of BAS, indicating the absence of OH-radical-scavengers in BAS.
Gerber
C E
CE
Department of Neonatology, Children's University Hospital, Rümelinstr. 23, Tübingen, D-72070, Germany. cagerber@med.uni-tuebingen.de
Bruchelt
G
G
Stegmann
H
H
Schweinsberg
F
F
Speer
C P
CP
D000418:Albumins / Q000378:metabolism; D001761:Bleomycin / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005293:Ferritins / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D017665:Hydroxyl Radical / Q000378:metabolism; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007781:Lactoferrin / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012127:Respiratory Distress Syndrome, Newborn / Q000209:etiology / Q000378:metabolism*; D014168:Transferrin / Q000378:metabolism; D019820:Xanthine / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D000418:Albumins; D017382:Reactive Oxygen Species; D014168:Transferrin; D001761:Bleomycin; D019820:Xanthine; D017665:Hydroxyl Radical; D005293:Ferritins; D006861:Hydrogen Peroxide; D007501:Iron; D014969:Xanthine Oxidase; D007781:Lactoferrin