Semiautomated metabolic staining assay for Bacillus cereus emetic toxin.
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.
Finlay
W J
WJ
School of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow G4 OBA, United Kingdom.
Logan
N A
NA
Sutherland
A D
AD
D001409:Bacillus cereus / Q000302:isolation & purification / Q000378:metabolism*; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000633:toxicity; D001431:Bacteriological Techniques; D004396:Coloring Agents / Q000378:metabolism; D004639:Emetics; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014407:Tumor Cells, Cultured; D014617:Vacuoles
D001427:Bacterial Toxins; D004396:Coloring Agents; D004639:Emetics; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue