title: Proof that the ACTVI genetic region of Streptomyces coelicolor A3(2) is involved in stereospecific pyran ring formation in the biosynthesis of actinorhodin.
abstract: Pyran ring formation in the biosynthesis of actinorhodin in Streptomyces coelicolor A3(2) was studied using the act cluster deficient strain, CH999, carrying pRM5-based plasmids harbouring combinations of the actVI genes. The strain, CH999/pIJ5660 (pRM5 + actVI-ORF1), produced a chiral intermediate, (S)-DNPA, suggesting that the actVI-ORF1 product is a reductase determining the C-3 stereochemical centre.
authors: Graduate School of Pharmaceutical Sciences, The University of Tokyo, Japan.
authors: K
authors: K
authors: Ichinose
authors: C
authors: C
authors: Surti
authors: T
authors: T
authors: Taguchi
authors: F
authors: F
authors: Malpartida
authors: K I
authors: KI
authors: Booker-Milburn
authors: G R
authors: GR
authors: Stephenson
authors: Y
authors: Y
authors: Ebizuka
authors: D A
authors: DA
authors: Hopwood
mesh_terms: D000880:Anthraquinones / Q000737:chemistry / Q000378:metabolism; D005798:Genes, Bacterial; D005810:Multigene Family; D016366:Open Reading Frames; D011714:Pyrans / Q000737:chemistry*; D015183:Restriction Mapping; D013302:Streptomyces / Q000235:genetics*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D011714:Pyrans; C013390:actinorhodin; D000095622:Benzoisochromanequinones