Thermobarostability of alpha-chymotrypsin in reversed micelles of aerosol OT in octane solvated by water-glycerol mixtures.
Thermostability of alpha-chymotrypsin at normal pressure in reversed micelles depends on both an effective surfactant solvation degree and glycerol content in the system. The difference in alpha-chymotrypsin stability in reversed micelles at various glycerol concentrations [up to 60% (v/v)] was more pronounced at high surfactant degrees of solvation, R >/= 16. After a 1-h incubation at 40 degrees C in "aqueous" reversed micelles (in the absence of glycerol), alpha-chymotrypsin retained only 1% of initial catalytic activity and 10, 22, 59, and 48% residual activity in glycerol-solvated micelles with 20, 30, 50, and 60% (v/v) glycerol, respectively. The explanation of the observed effects is given in the frames of micellar matrix structural order increasing in the presence of glycerol as a water-miscible cosolvent that leads to the decreasing mobility of the alpha-chymotrypsin molecule and, thus the increase of its stability. It was found that glycerol or hydrostatic pressure could be used to stabilize alpha-chymotrypsin in reversed micelles; a lower pressure is necessary to reach a given level of enzyme stability in the presence of glycerol.
Rariy
R V
RV
Division of Chemical Enzymology, Department of Chemistry, Moscow State University, 119899 Moscow, Russia.
Bec
N
N
Klyachko
N L
NL
Levashov
A V
AV
Balny
C
C
D000336:Aerosols / Q000737:chemistry; D002918:Chymotrypsin / Q000737:chemistry* / Q000378:metabolism*; D004143:Dioctyl Sulfosuccinic Acid / Q000737:chemistry; D004795:Enzyme Stability; D005947:Glucose / Q000737:chemistry; D005990:Glycerol / Q000737:chemistry; D008823:Micelles; D009795:Octanes / Q000737:chemistry; D011312:Pressure; D012997:Solvents / Q000737:chemistry*; D013696:Temperature; D014867:Water / Q000737:chemistry
D000336:Aerosols; D008823:Micelles; D009795:Octanes; D012997:Solvents; D014867:Water; D004143:Dioctyl Sulfosuccinic Acid; D002918:Chymotrypsin; D005947:Glucose; D005990:Glycerol; C026728:octane