[The loss of heterozygosity on the short arm of chromosome 3 in renal carcinoma].
OBJECTIVE
Renal cancer accounts for 2% of tumors. The most common chromosome abnormality found in renal cancer is the loss of heterozygosity (LOH) on the short arm of chromosome 3 (3p), which suggests that near the gene responsible for von Hippel-Lindau disease, there may be one or more tumor suppressor genes between 3p14 and 3p21 with a relevant role in the development of renal cancer.


METHODS
41 patients with sporadic renal cancer were tested for three microsatellites mapped to the short arm of chromosome 3 (3p14.1-3p14.3, 3p21.2-3p21.3 and 3p25) by polymerase chain reaction. The results were compared with patient habits and tumor features.


CONCLUSIONS
43.9% of the patients showed LOH on at least one locus. Thirty-four percent showed LOH only on one locus, 4.9% on two loci and 7.3% on the three loci tested. All the patients who showed LOH on 3p21 had a tumor size greater than 25 mm. There is a risk 1.76 times higher of no loss in tumors less than 25 mm in size than in tumors greater than 25 mm (Cl 95% 1.33-2.33).
Maestro de las Casas
M L
ML
Servicio de Análisis Clínicos, Biología Tumoral, Hospital Universitario San Carlos, Madrid.
del Barco Barriuso
V
V
Moreno Sierra
J
J
Izquierdo López
L
L
Sanz Casla
M T
MT
Zanna
I
I
Redondo González
E
E
Chicharro Almarza
J
J
Fernández Pérez
C
C
Resel Estévez
L
L
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001483:Base Sequence; D002292:Carcinoma, Renal Cell / Q000235:genetics*; D002893:Chromosomes, Human, Pair 3 / Q000235:genetics*; D017931:DNA Primers; D004273:DNA, Neoplasm / Q000235:genetics; D005260:Female; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics*; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D008875:Middle Aged; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods
D017931:DNA Primers; D004273:DNA, Neoplasm