Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons.
Previous studies have shown that brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) can enhance the survival of dopaminergic neurons in the ventral mesencephalon (VM). Here we compared several non-survival functions of the two factors in VM neurons in culture. We found that both BDNF and GDNF elicited an increase in the depolarization-induced release of dopamine, but had no effect on GABA release, in the VM cultures. BDNF, but not GDNF, significantly enhanced the expression of the calcium binding protein calbindin and synaptic protein SNAP25. In contrast, treatment of the cultures with GDNF, but not BDNF, elicited a marked fasciculation of the processes of the VM neurons. Thus, although both act on VM neurons, BDNF and GDNF have distinct functions.
Feng
L
L
Laboratory of Developmental Neurobiology, NICHD, NIH, Bethesda, MD 20892-4480, USA.
Wang
C Y
CY
Jiang
H
H
Oho
C
C
Mizuno
K
K
Dugich-Djordjevic
M
M
Lu
B
B
D000199:Actins / Q000096:biosynthesis; D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology*; D064026:Calbindins; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020934:Ciliary Neurotrophic Factor; D004298:Dopamine / Q000378:metabolism; D005333:Fetus / Q000166:cytology; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008565:Membrane Proteins / Q000096:biosynthesis; D008636:Mesencephalon / Q000166:cytology*; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000494:pharmacology*; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D018696:Neuroprotective Agents / Q000494:pharmacology*; D050683:R-SNARE Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley; D064030:S100 Calcium Binding Protein G / Q000096:biosynthesis; D013569:Synapses / Q000378:metabolism; D016704:Synapsins / Q000096:biosynthesis; D050825:Synaptosomal-Associated Protein 25; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000199:Actins; D019208:Brain-Derived Neurotrophic Factor; D064026:Calbindins; D020934:Ciliary Neurotrophic Factor; C495041:Gdnf protein, rat; D051100:Glial Cell Line-Derived Neurotrophic Factor; D008565:Membrane Proteins; D009414:Nerve Growth Factors; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D050683:R-SNARE Proteins; D064030:S100 Calcium Binding Protein G; C494210:Snap25 protein, rat; D016704:Synapsins; D050825:Synaptosomal-Associated Protein 25; D005680:gamma-Aminobutyric Acid; D004298:Dopamine