The physiological properties of human red cells as derived from kinetic osmotic volume changes.
Volume changes were originally used for studying the dynamic properties of glucose transport in red cells. As an extension it has been found possible to examine the interplay of three functional proteins evolved for the physiological role of human erythrocytes in transporting carbon dioxide and bicarbonate. The proteins chiefly concerned in this investigation were the cytoplasmic carbonic anhydrase and the two membrane transporting proteins, namely the band 3 anion exchanger and the unique bicarbonate transporter, which are distinct from the anion exchanger. The rates of anion membrane transport measured and the volume changes may be more than two orders of magnitude faster than those which regulate cationic movement in red cells, but this may only be an adaptation for the physiological role of red cells. The new concepts derived from the studies and their possible wider applications to physiological mechanisms are briefly discussed.
Widdas
W F
WF
Department of Biological Sciences, Royal Holloway College, University of London, Egham, Surrey, Great Britain.
Baker
G F
GF
D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid / Q000494:pharmacology; D001457:Anion Exchange Protein 1, Erythrocyte / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects / Q000502:physiology; D048429:Cell Size / Q000502:physiology; D002712:Chlorides / Q000378:metabolism; D004234:Diuretics, Osmotic / Q000494:pharmacology; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D005947:Glucose / Q000493:pharmacokinetics; D051272:Glucose Transporter Type 1; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000378:metabolism; D008353:Mannitol / Q000494:pharmacology; D000432:Methanol / Q000494:pharmacology; D009004:Monosaccharide Transport Proteins / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology; D014882:Water-Electrolyte Balance / Q000187:drug effects / Q000502:physiology*
D001457:Anion Exchange Protein 1, Erythrocyte; D002712:Chlorides; D004234:Diuretics, Osmotic; D051272:Glucose Transporter Type 1; D009004:Monosaccharide Transport Proteins; C495677:SLC2A1 protein, human; D012997:Solvents; D012856:4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; D019344:Lactic Acid; D008353:Mannitol; D012965:Sodium Chloride; D005947:Glucose; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D000432:Methanol