A stretch-sensitive Cl- channel in human corpus cavernosal myocytes.
With the patch clamp method we demonstrate a stretch-sensitive Cl- currents as well as stretch-sensitive Cl- channels in a small group (5%,n 117) of cultured human corpus cavernosal muscle cells. The current and the channel activities had the following characteristics: (1) Their equilibrium potentials changed with extracellular Cl- concentration close to that predicted by Nernst equation provided that the relevant channels had high permeability to Cl- but low permeability to acetate ions; (2) They were blocked by mM concentrations of Zn2+; (3) The i-v relation of single channel current was almost linear for holding potentials varied from -70 to +60 mV; and (4) The channels had unitary conductances of approximately 140-170 pS.
Fan
S F
SF
Department of Neurobiology and Behavior, State University of New York at Stony Brook, 11794-5230, USA.
Christ
G J
GJ
Melman
A
A
Brink
P R
PR
D000085:Acetates / Q000378:metabolism; D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000502:physiology*; D002463:Cell Membrane Permeability; D002478:Cells, Cultured; D018118:Chloride Channels / Q000502:physiology*; D002712:Chlorides / Q000378:metabolism / Q000494:pharmacology; D004553:Electric Conductivity; D006801:Humans; D008297:Male; D009130:Muscle, Smooth / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D010413:Penis / Q000737:chemistry* / Q000502:physiology / Q000648:ultrastructure; D010812:Physical Stimulation; D015032:Zinc / Q000494:pharmacology
D000085:Acetates; D018118:Chloride Channels; D002712:Chlorides; D015032:Zinc