A novel simple satellite DNA is colocalized with the Stalker retrotransposon in Drosophila melanogaster heterochromatin.
In the T(1:2)dor(var7) multibreak rearrangement the distal 1A-2B segment of the X chromosome of Drosophila melanogaster is juxtaposed to an inverted portion of the heterochromatin of chromosome 2. Analysis of mitotic chromosomes by a series of banding techniques has permitted us precisely to locate the heterochromatic breakpoint of this translocation in the h42 region of 2R. Cloning and sequencing of the eu-heterochromatic junction revealed that the translocated 1A-2B fragment is joined to (AACAC)n repeats, which represent a previously undescribed satellite DNA in D. melanogaster. These repeated sequences have been estimated to account for about 1 Mb of the D. melanogaster genome. The repeats are located mainly in the Y chromosome and in the heterochromatin of the right arm of chromosome 2 (2Rh), where they are colocalized with the Stalker retrotransposon.
Makunin
I V
IV
Institute of Cytology and Genetics, Novosibirsk, Russia.
Pokholkova
G V
GV
Kholodilov
N G
NG
Zakharkin
S O
SO
Bonaccorsi
S
S
Dimitri
P
P
Zhimulev
I F
IF
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D018076:DNA, Complementary; D004276:DNA, Satellite; D004331:Drosophila melanogaster / Q000235:genetics*; D015321:Gene Rearrangement; D017344:Genes, Insect; D006570:Heterochromatin; D008938:Mitosis; D008969:Molecular Sequence Data; D018626:Retroelements; D017422:Sequence Analysis, DNA
D018076:DNA, Complementary; D004276:DNA, Satellite; D006570:Heterochromatin; D018626:Retroelements