Requirement of type III TGF-beta receptor for endocardial cell transformation in the heart.
Transforming growth factor-beta (TGF-beta) signaling is mediated by a complex of type I (TBRI) and type II (TBRII) receptors. The type III receptor (TBRIII) lacks a recognizable signaling domain and has no clearly defined role in TGF-beta signaling. Cardiac endothelial cells that undergo epithelial-mesenchymal transformation express TBRIII, and here TBRIII-specific antisera were found to inhibit mesenchyme formation and migration in atrioventricular cushion explants. Misexpression of TBRIII in nontransforming ventricular endothelial cells conferred transformation in response to TGF-beta2. These results support a model where TBRIII localizes transformation in the heart and plays an essential, nonredundant role in TGF-beta signaling.
Brown
C B
CB
Department of Pharmacology, Vanderbilt University Medical Center, 1161 21st Avenue South, Nashville, TN 37232-6600, USA.
Boyer
A S
AS
Runyan
R B
RB
Barnett
J V
JV
D000818:Animals; D002465:Cell Movement; D002642:Chick Embryo; D046508:Culture Techniques; D004699:Endocardium / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D004727:Endothelium / Q000166:cytology* / Q000196:embryology / Q000378:metabolism; D005822:Genetic Vectors; D006321:Heart / Q000196:embryology*; D006325:Heart Atria / Q000166:cytology / Q000196:embryology; D006352:Heart Ventricles / Q000166:cytology / Q000196:embryology / Q000821:virology; D007106:Immune Sera; D008024:Ligands; D008648:Mesoderm / Q000166:cytology* / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases; D011509:Proteoglycans / Q000276:immunology / Q000502:physiology*; D000077294:Receptor, Transforming Growth Factor-beta Type II; D018125:Receptors, Transforming Growth Factor beta / Q000276:immunology / Q000502:physiology*; D012190:Retroviridae / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000378:metabolism* / Q000494:pharmacology
D007106:Immune Sera; D008024:Ligands; D011509:Proteoglycans; D018125:Receptors, Transforming Growth Factor beta; D016212:Transforming Growth Factor beta; C071736:betaglycan; D017346:Protein Serine-Threonine Kinases; D000077294:Receptor, Transforming Growth Factor-beta Type II