The effects of compression sutures on filtering blebs in rabbit eyes.
OBJECTIVE
To investigate the effect of compression sutures on filtering bleb cellularity in rabbit eyes.


METHODS
Six New Zealand rabbits had bilateral filtering surgery. In the study eye, on postoperative day 14 a 10-0 nylon suture was placed that compressed the bleb. Five-bromo-2'-deoxyuridine (BrdU) was injected intravenously on days 15, 16, and 18. On day 21 histologic sections of the blebs were obtained. The total cell count was compared to the number of actively dividing cells (marked by anti-BrdU antibody).


RESULTS
Study eyes had higher total cell counts (P = .004) on postoperative day 21, but the proportion of proliferating cells was not significantly different (P = .11).


CONCLUSIONS
In rabbit eyes, bleb compression sutures were associated with more cellular components in the bleb compared to control eyes, but did not induce a higher level of cellular proliferation.
Chen
P P
PP
Department of Ophthalmology, University of Washington, Seattle 98195-6485, USA.
Takahashi
Y
Y
Leen
M M
MM
Bhandari
A
A
Li
Y
Y
Mills
R P
RP
D000818:Animals; D000869:Anterior Eye Segment / Q000473:pathology / Q000601:surgery*; D002452:Cell Count; D002455:Cell Division; D018463:Filtering Surgery; D011817:Rabbits; D012086:Reoperation; D012590:Sclera / Q000473:pathology / Q000601:surgery; D013536:Suture Techniques; D016896:Treatment Outcome