Suppressor analysis of the Saccharomyces cerevisiae gene REC104 reveals a genetic interaction with REC102.
REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae. To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for high-copy suppressors. An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles. However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not "bypass" suppression. Overexpression of the early meiotic genes REC114, RAD50, HOP1, and RED1 fails to suppress any of the rec104 conditional alleles, indicating that the suppression might be specific to REC102.
Salem
L
L
Program in Genetics, University of Iowa, Iowa City, Iowa 52242, USA.
Walter
N
N
Malone
R
R
D000483:Alleles; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins; D005656:Fungal Proteins / Q000235:genetics; D005800:Genes, Fungal; D008540:Meiosis / Q000235:genetics; D016296:Mutagenesis; D009154:Mutation; D016133:Polymerase Chain Reaction; D045522:Recombinases; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins; D013489:Suppression, Genetic; D013696:Temperature
D017931:DNA Primers; D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C073372:REC102 protein, S cerevisiae; C080594:REC104 protein, S cerevisiae; D045522:Recombinases; D029701:Saccharomyces cerevisiae Proteins