title: Evidence for the extended phospholipid conformation in membrane fusion and hemifusion.
abstract: Molecular-level mechanisms of fusion and hemifusion of large unilamellar dioleoyl phosphatidic acid/phosphocholine (DOPA/DOPC, 1:1 molar ratio) vesicles induced by millimolar Ca2+ and Mg2+, respectively, were investigated using fluorescence spectroscopy. In keeping with reduction of membrane free volume Vf, both divalent cations increased the emission polarization for 1,6-diphenyl-1,3, 5-hexatriene (DPH). An important finding was a decrease in excimer/monomer emission intensity ratio (Ie/Im) for the intramolecular excimer-forming probe 1, 2-bis[(pyren-1-)yl]decanoyl-sn-glycero-3-phosphocholine (bis-PDPC) in the course of fusion and hemifusion. Comparison with another intramolecular excimer-forming probe, namely, 1-[(pyren-1)-yl]decanoyl-2-[(pyren-1)-yl]tetradecanoyl-sn-gl ycero-3-p hosphocholine (PDPTPC), allowed us to exclude changes in acyl chain alignment to be causing the decrement in Ie/Im. As a decrease in Vf should increase Ie/Im for bis-PDPC and because contact site between adhering liposomes was required we conclude the most feasible explanation to be the adoption of the extended conformation (P.K.J., Chem. Phys. Lipids 63:251-258) by bis-PDPC. In this conformation the two acyl chains are splaying so as to become embedded in the opposing leaflets of the two adhered bilayers, with the headgroup remaining between the adjacent surfaces. Our data provide evidence for a novel mechanism of fusion of the lipid bilayers.
authors: Biomembrane Research Group, Department of Medical Chemistry, Institute of Biomedicine, University of Helsinki, FIN-00014 Helsinki, Finland.
authors: J M
authors: JM
authors: Holopainen
authors: J Y
authors: JY
authors: Lehtonen
authors: P K
authors: PK
authors: Kinnunen
mesh_terms: D055592:Biophysical Phenomena; D001703:Biophysics; D005454:Fluorescence Polarization; D005456:Fluorescent Dyes; D066298:In Vitro Techniques; D008081:Liposomes / Q000737:chemistry; D008561:Membrane Fusion / Q000502:physiology*; D008968:Molecular Conformation; D010712:Phosphatidic Acids / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry*
chemical_list: C086132:1,2-bis(pyren-1-yl)decanoyl-sn-glycero-3-phosphocholine; D005456:Fluorescent Dyes; D008081:Liposomes; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; C037657:dioleoylphosphatidic acid; C017251:1,2-oleoylphosphatidylcholine