Functional analysis of T lymphocytes infiltrating the dermis and epidermis of post-burn hypertrophic scar tissues.
The cytokine profile of T cell clones (TCC) from the dermis and epidermis of burn patients with hypertrophic scars (HS) in active (AHS) and remission phases (RHS) was determined in this study. We found that AHS tissues are heavily infiltrated by Type 0-Type 1 polarized CD3+ lymphocytes producing high IFN-gamma and low IL-4 levels. Analysis of their surface marker phenotype showed that the high IFN-gamma production was shared equally between the CD4+ TCRalpha/beta and CD8+ TCRalpha/beta clones. The profile of TCC from RHS tissues revealed pronounced infiltration of Type 0-Type 1 polarized lymphocytes with an even more evident Type 1 profile. However, the levels of IFN-gamma produced by RHS-derived TCC were 4-6 times lower than those produced by AHS-derived TCC. These data show that high levels of IFN-gamma produced by Type 0-Type 1 lymphocytes infiltrating HS are a feature of AHS, whereas reduction of this ability to produce high levels of IFN-gamma, though without a shift towards a Type 0-Type 2 phenotype through an increase in IL-4, is characteristic of RHS.
Bernabei
P
P
Department of Clinical and Biological Sciences, University of Turin, Orbassano, Italy.
Rigamonti
L
L
Ariotti
S
S
Stella
M
M
Castagnoli
C
C
Novelli
F
F
D002056:Burns / Q000150:complications / Q000276:immunology / Q000473:pathology*; D015704:CD4 Antigens / Q000032:analysis; D016827:CD8 Antigens / Q000032:analysis; D017439:Cicatrix, Hypertrophic / Q000209:etiology / Q000276:immunology / Q000473:pathology*; D020405:Dermis / Q000276:immunology / Q000473:pathology; D004817:Epidermis / Q000276:immunology / Q000473:pathology; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000032:analysis*; D012867:Skin / Q000276:immunology / Q000473:pathology*; D016176:T-Lymphocyte Subsets; D013601:T-Lymphocytes / Q000276:immunology / Q000473:pathology*
D015704:CD4 Antigens; D016827:CD8 Antigens; D016693:Receptors, Antigen, T-Cell, alpha-beta; D015847:Interleukin-4; D007371:Interferon-gamma