Stoichiometry of dipalmitoylphosphatidylcholine-DNA interaction in the presence of Ca2+: a temperature-scanning ultrasonic study.
DNA-DPPC complexes can be prepared by means of a single step procedure of mixing DNA solution and aqueous lipid dispersion in the presence of calcium ions. Interaction between DPPC and DNA brings about a biphasic shape of melting curves corresponding to the free lipid and the strongly bound one. The amount of the strongly bound lipid is 5 molecules per nucleotide which is close to the size of the first lipid monolayer around DNA molecule.
Kharakoz
D P
DP
Institute of Theoretical and Experimental Biophysics, Pushchino, Russia. kharakoz@pbc.iteb.serpukhov.su
Khusainova
R S
RS
Gorelov
A V
AV
Dawson
K A
KA
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D002417:Cattle; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D007368:Interferometry; D013696:Temperature; D014465:Ultrasonics
D015060:1,2-Dipalmitoylphosphatidylcholine; D004247:DNA; D002118:Calcium