A highly efficient preparation of neoglycoconjugate vaccines using subcarriers that bear clustered carbohydrate antigens.
A limited amount of spacer-equipped carbohydrate haptens was linked by reductive amination to a subcarrier, an oligopeptide containing 16 amino groups, to give a hapten-carrying subcarrier (HCS). It was then linked, via the remaining free amino groups, to chicken serum albumin (CSA) to give a cross-linked neoglycoconjugate bearing the haptens in the form of clusters. Alternatively, the same type of a glycoconjugate, but with higher conjugation efficiency, was obtained when HCS was treated successively with squaric acid diethyl ester and CSA.
Zhang
J
J
NIDDK, National Institutes of Health, Bethesda, MD 20892-0815, USA.
Kovác
P
P
D000941:Antigens / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002241:Carbohydrates / Q000737:chemistry*; D006001:Glycoconjugates / Q000737:chemistry*; D006241:Haptens / Q000737:chemistry; D014612:Vaccines / Q000737:chemistry*
D000941:Antigens; D002241:Carbohydrates; D006001:Glycoconjugates; D006241:Haptens; D014612:Vaccines