title: Mutation analysis of the Fanconi anaemia A gene in breast tumours with loss of heterozygosity at 16q24.3.
abstract: The recently identified Fanconi anaemia A (FAA) gene is located on chromosomal band 16q24.3 within a region that has been frequently reported to show loss of heterozygosity (LOH) in breast cancer. FAA mutation analysis of 19 breast tumours with specific LOH at 16q24.3 was performed. Single-stranded conformational polymorphism (SSCP) analysis on cDNA and genomic DNA, and Southern blotting failed to identify any tumour-specific mutations. Five polymorphisms were identified, but frequencies of occurrence did not deviate from those in a normal control population. Therefore, the FAA gene is not the gene targeted by LOH at 16q24.3 in breast cancer. Another tumour suppressor gene in this chromosomal region remains to be identified.
authors: Department of Pathology, Leiden University Medical Center, The Netherlands.
authors: A M
authors: AM
authors: Cleton-Jansen
authors: E W
authors: EW
authors: Moerland
authors: J C
authors: JC
authors: Pronk
authors: C G
authors: CG
authors: van Berkel
authors: S
authors: S
authors: Apostolou
authors: J
authors: J
authors: Crawford
authors: A
authors: A
authors: Savoia
authors: A D
authors: AD
authors: Auerbach
authors: C G
authors: CG
authors: Mathew
authors: D F
authors: DF
authors: Callen
authors: C J
authors: CJ
authors: Cornelisse
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D018797:Cell Cycle Proteins; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics*; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins; D051856:Fanconi Anemia Complementation Group Proteins; D005260:Female; D016147:Genes, Tumor Suppressor / Q000235:genetics*; D006801:Humans; D019656:Loss of Heterozygosity; D009687:Nuclear Proteins; D018807:Polymorphism, Single-Stranded Conformational; D011506:Proteins / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D051856:Fanconi Anemia Complementation Group Proteins; D009687:Nuclear Proteins; D011506:Proteins