Distribution of interleukin-2 receptor alpha-chain and cells expressing major histocompatibility complex class II antigen in chronic human periapical lesions.
In situ distribution of CD2+ T-lymphocytes, CD4+ and CD8+ T-cell subsets, CD14+ macrophages, interleukin-2 receptor alpha-chain (IL-2R alpha) and class II major histocompatibility complex antigen (major histocompatibility complex class II, HLA-DR) expressing cells were determined in 14 chronic human periapical granulomas by immunohistochemical method using monoclonal antibodies. CD2+ lymphocytes were rather evenly distributed within the classical granulation tissue and comprised 55% of the mononuclear cells. Macrophages were distributed all over the periapical area, but their proportion was much less than that of T lymphocytes. Both small, lymphocyte-like mononuclear cells and larger mononuclear cells resembling macrophages displayed mild to strong circumferential staining with the anti-HLA-DR antibody. The majority of lymphocytes expressed IL-2R alpha indicating the activated state of T cells within the lesion.
Márton
I J
IJ
Department of Restorative Dentistry, University Medical School of Debrecen, Hungary.
Dezsö
B
B
Radics
T
T
Kiss
C
C
D000911:Antibodies, Monoclonal; D018801:CD2 Antigens / Q000032:analysis; D016516:CD4-CD8 Ratio; D002908:Chronic Disease; D006684:HLA-DR Antigens / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation; D008264:Macrophages; D010484:Periapical Granuloma / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D016176:T-Lymphocyte Subsets
D000911:Antibodies, Monoclonal; D018801:CD2 Antigens; D006684:HLA-DR Antigens; D011948:Receptors, Antigen, T-Cell; D015375:Receptors, Interleukin-2