title: Genetic optimization of recombinant glycoprotein production by mammalian cells.
abstract: Genetically modified mammalian cells are the preferred system for the production of recombinant therapeutic glycoproteins. Other applications include engineering of cell lines for drug screening and cell-based therapies, and the construction of recombinant viruses for gene therapy. This article highlights contemporary core genetic technologies and emerging strategies for genetically engineering mammalian cells for optimal recombinant-protein expression.
authors: Institute of Biotechnology, ETH Hönggerberg, Zurich, Switzerland.
authors: M
authors: M
authors: Fussenegger
authors: J E
authors: JE
authors: Bailey
authors: H
authors: H
authors: Hauser
authors: P P
authors: PP
authors: Mueller
mesh_terms: D017209:Apoptosis / Q000235:genetics; D005786:Gene Expression Regulation; D005796:Genes / Q000235:genetics; D006023:Glycoproteins / Q000096:biosynthesis*; D006031:Glycosylation; D008967:Molecular Biology / Q000379:methods*; D015202:Protein Engineering / Q000379:methods*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification; D011995:Recombination, Genetic; D014158:Transcription, Genetic
chemical_list: D006023:Glycoproteins; D011994:Recombinant Proteins