Time course of SDS-alkaline lysis of recombinant bacterial cells for plasmid release.
SDS-alkaline lysis of recombinant Escherichia coli cell suspensions was carried out in a coaxial cylinder rheometer, and the data were used to establish the time course of lysis reaction. The results of the experiments showed that cell lysis reaction time depended on cell strain but was unaffected by plasmid size and plasmid copy number. The high molecular weight globular proteins and chromosomal DNA were denatured, and the resulting changes in rheometric measurements characterised the denaturation time.
Ciccolini
L A
LA
The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, England.
Shamlou
P A
PA
Titchener-Hooker
N J
NJ
Ward
J M
JM
Dunnill
P
P
D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010957:Plasmids / Q000378:metabolism*; D011995:Recombination, Genetic; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors; D014783:Viscosity
D012967:Sodium Dodecyl Sulfate