A method of computationally enhanced detection of chromatogram regions with apparent high relative agonist activity.
An occasional but difficult problem arises in drug discovery during a chromatographic analysis in which high background activity is associated with the presence of most eluting molecular species. This makes the isolation of material of high relative activity difficult. A computational method is shown that clarifies the identification of regions of the chromatogram of interest. The data for bioactivity and absorbance are normalized to percent of maximal response, filtered to raise very small or zero values to a minimal level, and the activity/absorbance ratio is plotted per fraction. The fractions with relatively high activity become evident. This technique is a helpful adjunct to existing graphical methods and provides an objective relationship between the data sets. It is simple to implement with Visual Basic and spreadsheet data, making it widely accessible.
Price
J A
JA
Department of Biochemistry and Microbiology, Oklahoma State University College of Osteopathic Medicine, Tulsa 74107, USA. pjoseph@osucom-fs02.ocom.okstate.edu
D000818:Animals; D002845:Chromatography / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D001330:Electronic Data Processing / Q000379:methods*; D006632:Histamine / Q000032:analysis*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012910:Snake Venoms; D012911:Snakes
D012910:Snake Venoms; D012710:Serum Albumin, Bovine; D006632:Histamine