Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization.
A cDNA, recently cloned (by Krieg et al. (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid were substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kb. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11.
Krieg
P
P
Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany. p.krieg@dkfz-heidelberg.de
Siebert
M
M
Kinzig
A
A
Bettenhausen
R
R
Marks
F
F
Fürstenberger
G
G
D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
D018076:DNA, Complementary; D011994:Recombinant Proteins; D001092:Arachidonate 12-Lipoxygenase