title: Abnormal photoresponses and light-induced apoptosis in rods lacking rhodopsin kinase.
abstract: Phosphorylation is thought to be an essential first step in the prompt deactivation of photoexcited rhodopsin. In vitro, the phosphorylation can be catalyzed either by rhodopsin kinase (RK) or by protein kinase C (PKC). To investigate the specific role of RK, we inactivated both alleles of the RK gene in mice. This eliminated the light-dependent phosphorylation of rhodopsin and caused the single-photon response to become larger and longer lasting than normal. These results demonstrate that RK is required for normal rhodopsin deactivation. When the photon responses of RK-/- rods did finally turn off, they did so abruptly and stochastically, revealing a first-order backup mechanism for rhodopsin deactivation. The rod outer segments of RK-/- mice raised in 12-hr cyclic illumination were 50% shorter than those of normal (RK+/+) rods or rods from RK-/- mice raised in constant darkness. One day of constant light caused the rods in the RK-/- mouse retina to undergo apoptotic degeneration. Mice lacking RK provide a valuable model for the study of Oguchi disease, a human RK deficiency that causes congenital stationary night blindness.
authors: Division of Biology, 147-75, California Institute of Technology, Pasadena, CA 91125, USA.
authors: C K
authors: CK
authors: Chen
authors: M E
authors: ME
authors: Burns
authors: M
authors: M
authors: Spencer
authors: G A
authors: GA
authors: Niemi
authors: J
authors: J
authors: Chen
authors: J B
authors: JB
authors: Hurley
authors: D A
authors: DA
authors: Baylor
authors: M I
authors: MI
authors: Simon
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D003624:Darkness; D005136:Eye Proteins; D051606:G-Protein-Coupled Receptor Kinase 1; D006720:Homozygote; D006801:Humans; D008027:Light; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D010766:Phosphorylation; D010775:Photic Stimulation; D017440:Photoperiod; D011493:Protein Kinase C / Q000378:metabolism; D011494:Protein Kinases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D017948:Retinal Rod Photoreceptor Cells / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects; D012243:Rhodopsin / Q000378:metabolism; D012374:Rod Cell Outer Segment / Q000166:cytology / Q000502:physiology / Q000528:radiation effects
chemical_list: D005136:Eye Proteins; D012243:Rhodopsin; D011494:Protein Kinases; D011493:Protein Kinase C; D051606:G-Protein-Coupled Receptor Kinase 1; C496764:GRK1 protein, human; C496762:Grk1 protein, mouse