title: Advantages in using immobilized thermophilic beta-glycosidase in nonisothermal bioreactors.
abstract: Catalytic membranes, obtained by immobilizing thermophilic beta-glycosidase onto nylon supports, were used in a nonisothermal bioreactor to study the effect of temperature gradients on the rate of enzyme reaction. Two experimental approaches were carried out to explain the molecular mechanisms by which the temperature gradients affect enzyme activity. The results showed that the thermophilic enzyme behaved as the mesophilic beta-galactosidase, exhibiting an activity increase which was linearly proportional to the transmembrane temperature difference. The efficiency of the system proposed was determined by calculating two constants, alpha and beta, which represent respectively the percentage increase of enzyme activity when a temperature difference of 1 degrees C or a temperature gradient of 1 degrees C cm-1 were applied across the catalytic membrane. The increase of enzyme activity in nonisothermal bioreactors entailed a proportional reduction of production times. The advantages in using thermophilic enzymes immobilized in nonisothermal bioreactors are also discussed.
authors: Istituto Internazionale di Genetica e Biofisica, CNR, via G. Marconi 12, 80125 Napoli, Italy.
authors: F
authors: F
authors: Febbraio
authors: M
authors: M
authors: Portaccio
authors: S
authors: S
authors: Stellato
authors: S
authors: S
authors: Rossi
authors: U
authors: U
authors: Bencivenga
authors: R
authors: R
authors: Nucci
authors: M
authors: M
authors: Rossi
authors: F S
authors: FS
authors: Gaeta
authors: D G
authors: DG
authors: Mita
mesh_terms: D019149:Bioreactors; D002384:Catalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004867:Equipment Design; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007700:Kinetics; D008567:Membranes, Artificial; D011138:Polytetrafluoroethylene; D011994:Recombinant Proteins / Q000378:metabolism; D013816:Thermodynamics
chemical_list: D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D011994:Recombinant Proteins; D011138:Polytetrafluoroethylene; D006026:Glycoside Hydrolases