On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements.
The use of on-line relative permittivity (epsilon') measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing beta-galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in epsilon' in both non-infected and synchronously infected cultures, indicating that the epsilon' of a cell suspension was sensitive only to changes in the viable cell population. Additionally the parameter provided clearly defined signposts of the progress of the infection.
Zeiser
A
A
Animal Cell Technology and Downstream Processing Group, Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montréal, Québec, Canada, H4P 2R2.
Bédard
C
C
Voyer
R
R
Jardin
B
B
Tom
R
R
Kamen
A A
AA
D000818:Animals; D016367:Baculoviridae; D018929:Cell Culture Techniques / Q000379:methods*; D002455:Cell Division; D002470:Cell Survival; D007700:Kinetics; D008954:Models, Biological; D009862:Online Systems; D011994:Recombinant Proteins / Q000096:biosynthesis; D018411:Spodoptera; D013997:Time Factors; D014162:Transfection; D001616:beta-Galactosidase / Q000096:biosynthesis* / Q000235:genetics
D011994:Recombinant Proteins; D001616:beta-Galactosidase