Effect of magnetic resonance imaging on human respiratory burst of neutrophils.
It is known that low intensity magnetic fields increase superoxide anion production during the respiratory burst of rat peritoneal neutrophils in vitro. We investigated whether the high intensity magnetic fields (1.5 T) during magnetic resonance imaging can influence the human neutrophil function under in vivo conditions. Blood samples were obtained from 12 patients immediately before and after magnetic resonance imaging (mean time 27.6(+/-11.4 min)). The induced respiratory burst was investigated by the intracellular oxidative transformation of dihydrorhodamine 123 to the fluorescent dye rhodamine 123 via flow cytometry. The respiratory burst was induced either with phorbol 12-myristate 13-acetate, Escherichia coli, N-formyl-methionyl-leucylphenylalanine or priming with tumor necrosis factor followed by FMLP stimulation. There was no significant difference between the respiratory burst before and after magnetic resonance imaging, irrespective of the stimulating agent. Short time exposure to a high intensity magnetic field during magnetic resonance imaging seems not to influence the production of radical species in living neutrophils.
Heine
J
J
Department of Neuroradiology, Hannover Medical School, Germany. heine.joern@mh-hannover.de
Scheinichen
D
D
Jaeger
K
K
Herzog
T
T
Sümpelmann
R
R
Leuwer
M
M
D000328:Adult; D000818:Animals; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000378:metabolism / Q000528:radiation effects*; D051381:Rats; D016897:Respiratory Burst / Q000528:radiation effects*