[Influence of abnormal structure beta chain of luteinizing hormone on endocrinological kinetics of luteinizing hormone and gynecologic diseases].
With recent progress in endocrinology and in procreation physiology, the importance of kinetics of pituitary gonadotropin has been increasing, and the measurement method has been improved. In the present study, however, we found inconsistency in measured LH values between IRMA (SPAC-S LH) as the conventional method and CLEIA (IMMULYZE LH) as the newly developed method. The inconsistency between the SPAC-S LH value and the IMMULYZE LH value was observed in 10.0% of the healthy group and in 12.5% of the patient group. The cause of this discrepancy was due to a reaction of the SPAC-S LH of the intact LH monoclonal antibodies to the LH with the abnormal structure beta chain by two point mutation in the LH beta gene. The response of LH-RH test varied depending on the measurement reagent of LH in patients who had the LH with the abnormal structure beta chain, which made it difficult to determine the lesion and histological grading regarding the ovulation mechanism. Therefore, in patients with abnormal beta chain, an accurate treatment protocol was indeterminate. In this study, although a relationship between various gynecological diseases and the point mutation of LH was not clarified, we suggest that LH of the abnormal structure beta chain may cause excessive secretion in the early stage, and lead to some effect on physical activities.
Karino
K
K
Central Clinical Laboratory, Shimane Medical University, Izumo.
Shibata
H
H
Takahashi
K
K
Masuda
J
J
D015415:Biomarkers / Q000097:blood; D005260:Female; D005831:Genital Diseases, Female / Q000175:diagnosis*; D007987:Gonadotropin-Releasing Hormone; D006801:Humans; D007124:Immunoenzyme Techniques; D015592:Immunoradiometric Assay; D008163:Luminescent Measurements; D007986:Luteinizing Hormone / Q000097:blood* / Q000737:chemistry / Q000235:genetics; D008297:Male; D010050:Ovarian Function Tests / Q000379:methods; D017354:Point Mutation
D015415:Biomarkers; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone