Genetic relatedness between oral and intestinal isolates of Porphyromonas endodontalis by analysis of random amplified polymorphic DNA.
Genomic fingerprints from the DNA of 27 strains of Porphyromonas endodontalis from diverse clinical and geographic origins were generated as random amplified polymorphic DNA (RAPD) using the technique of PCR amplification with a single primer of arbitrary sequence. Cluster analysis of the combined RAPD data obtained with three selected 9- or 10-mer-long primers identified 25 distinct RAPD types which clustered as three main groups identifying three genogroups. Genogroups I and II included exclusively P. endodontalis isolates of oral origin, while 7/9 human intestinal strains of genogroup III which linked at a similarity level of 52% constituted the most homogeneous group in our study. Genotypic diversity within P. endodontalis, as shown by RAPD analysis, suggests that the taxon is composed of two oral genogroups and one intestinal genogroup. This hypothesis remains to be confirmed.
Gonçalves
R B
RB
Groupe de Recherche en Ecologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec, Canada. reginaldobruno@yahoo.com
Väisänen
M L
ML
Van Steenbergen
T J
TJ
Sundqvist
G
G
Mouton
C
C
D000818:Animals; D016172:DNA Fingerprinting; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D009055:Mouth / Q000382:microbiology*; D017861:Porphyromonas / Q000235:genetics*