Effect of staurosporine on outflow facility in monkeys.
OBJECTIVE
To determine the effect of the serine-threonine kinase inhibitor staurosporine on outflow facility in living monkeys.


METHODS
Total outflow facility was determined by two-level constant pressure perfusion of the anterior chamber bilaterally before and after intracameral infusion of staurosporine or vehicle in opposite eyes.


RESULTS
Intracameral staurosporine dose-dependently doubled outflow facility, with 0.1 microM, 1 microM, and 10 microM being subthreshold, effective, and maximal doses, respectively. At 50 microM, intracameral staurosporine was less effective than 10 microM on facility and induced corneal toxicity.


CONCLUSIONS
The broad-spectrum protein kinase inhibitor staurosporine increases outflow facility in living monkeys, perhaps by affecting the trabecular meshwork cytoskeleton.
Tian
B
B
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison 53792-3220, USA.
Gabelt
B T
BT
Kaufman
P L
PL
D000818:Animals; D000867:Anterior Chamber / Q000187:drug effects; D001082:Aqueous Humor / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D008252:Macaca fascicularis; D008297:Male; D009883:Ophthalmic Solutions / Q000008:administration & dosage / Q000494:pharmacology; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors; D019311:Staurosporine / Q000008:administration & dosage / Q000494:pharmacology*; D014129:Trabecular Meshwork / Q000187:drug effects* / Q000378:metabolism
D004791:Enzyme Inhibitors; D009883:Ophthalmic Solutions; D017346:Protein Serine-Threonine Kinases; D019311:Staurosporine