Multilineage potential of adult human mesenchymal stem cells.
Human mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells were isolated from marrow aspirates of volunteer donors. These cells displayed a stable phenotype and remained as a monolayer in vitro. These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential.
Pittenger
M F
MF
Osiris Therapeutics, 2001 Aliceanna Street, Baltimore, MD 21231-3043, USA. mpittenger@osiristx.com
Mackay
A M
AM
Beck
S C
SC
Jaiswal
R K
RK
Douglas
R
R
Mosca
J D
JD
Moorman
M A
MA
Simonetti
D W
DW
Craig
S
S
Marshak
D R
DR
D017667:Adipocytes / Q000166:cytology*; D000328:Adult; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D002469:Cell Separation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology; D005434:Flow Cytometry; D006801:Humans; D008648:Mesoderm / Q000166:cytology*; D008875:Middle Aged; D010011:Osteocytes / Q000166:cytology*; D010641:Phenotype; D013234:Stem Cells / Q000166:cytology*