Generation of infectious genome of bovine adenovirus type 3 by homologous recombination in bacteria.
The widely used technique of generating adenovirus vectors by homologous recombination in mammalian cells is usually not very efficient. This communication describes a simple method of generating a plasmid containing the full-length genome of an adenovirus by homologous recombination in bacteria. Following transfection of a suitable mammalian cell line with the full-length adenovirus genome, infectious virus progeny could easily be generated. Using this technique the generation of adenovirus recombinants would be efficient and straightforward.
van Olphen
A L
AL
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907-1243, USA.
Mittal
S K
SK
D000818:Animals; D002417:Cattle; D004926:Escherichia coli / Q000235:genetics*; D016679:Genome, Viral; D017921:Mastadenovirus / Q000235:genetics* / Q000472:pathogenicity; D010957:Plasmids; D011995:Recombination, Genetic