Molecular cloning of rat GADD45gamma, gene induction and its role during neuronal cell death.
To study the molecular mechanism of neuronal cell death, we carried out the screening of genes which were induced during the neuronal cell death of neuronal PC12. We cloned the cDNA of rat GADD45gamma, the third member of the GADD45 family. Induction of GADD45gamma mRNA was observed in the neuronal cell death caused by depletion of neurotrophic factor and Ca2+ ionophore treatment. Overexpression of GADD45gamma in superior cervical ganglion neurons caused cell death. These results suggest that GADD45gamma plays an important role in neuronal cell death.
Kojima
S
S
Shionogi Institute for Medical Science, Osaka, Japan.
Mayumi-Matsuda
K
K
Suzuki
H
H
Sakata
T
T
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016923:Cell Death; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D047908:Intracellular Signaling Peptides and Proteins; D008969:Molecular Sequence Data; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D011506:Proteins / Q000235:genetics* / Q000502:physiology*; D051381:Rats; D017386:Sequence Homology, Amino Acid; D015533:Transcriptional Activation; D000097642:GADD45 Proteins
D018076:DNA, Complementary; D047908:Intracellular Signaling Peptides and Proteins; D011506:Proteins; D000097642:GADD45 Proteins