Molecular typing of Salmonella serotype Thompson strains isolated from human and animal sources.
One-hundred-and-thirteen isolates of Salmonella serotype Thompson from diverse sources in seven countries were characterized by PvuII ribotyping and IS200 fingerprinting. Ten PvuII ribotypes were observed. The predominant PvuII ribotype 1 represented a major clone of world-wide distribution but was not found in Australia; PvuII ribotypes 2 and 3 represented minor clones. HincII ribotyping discriminated subtypes within PvuII ribotype 1: HincII ribotype 1 was distributed widely but HincII ribotype 2 was found mainly in Scottish isolates. None of 101 isolates of PvuII ribotypes 1-3 contained copies of IS200. All 12 isolates of PvuII ribotypes 4-10 were from Australia and 7 of them contained copies of IS200 of 5 different profiles. These results suggest the existence of at least two lineages of Salmonella Thompson with a different geographical distribution. The finding that most isolates from man and poultry in Scotland belonged to the same ribotype (PvuII 1/HincII 2) and were IS200-negative suggests that poultry is an important source of human infection in Scotland.
Chisholm
S A
SA
Department of Medical Microbiology, University of Dundee Medical School, Ninewells Hospital, Scotland.
Crichton
P B
PB
Knight
H I
HI
Old
D C
DC
D000818:Animals; D001315:Australia; D002170:Canada; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific; D016002:Discriminant Analysis; D004739:England; D005602:France; D006801:Humans; D007557:Israel; D017720:Molecular Epidemiology; D011200:Poultry / Q000382:microbiology; D015183:Restriction Mapping / Q000379:methods*; D019779:Salmonella enterica / Q000145:classification* / Q000235:genetics*; D012606:Scotland; D012703:Serotyping / Q000379:methods*; D014481:United States
D004269:DNA, Bacterial; C025630:CAGCTG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific; C024400:GTYRAC-specific type II deoxyribonucleases