Expression of Drosophila trithorax-group homologues in chick embryos.
Mll, Brg1 and Brm are vertebrate homologues of Drosophila trithorax group (trxG) genes. We isolated chicken Mll cDNA clones, and examined patterns of Mll, Brg1 and Brm expression in chick embryos. All three genes were expressed from embryonic stage 2 onwards. Mll transcripts were just detectable in all tissues by in situ hybridization, with highest level in dorsal neural tube and notochord. Brg1 transcripts were readily detectable in all tissues, with highest levels in dorsal neural tube, dorsal trunk epithelium and limb bud epithelium and mesenchyme. Brm transcripts were more restricted, being found in dermomyotome, notochord, dorsal limb bud epithelium, eye and the roof and floor plates of the neural tube.
Schofield
J
J
Molecular Haematology Unit, Institute of Child Health, London, UK.
Isaac
A
A
Golovleva
I
I
Crawley
A
A
Goodwin
G
G
Tickle
C
C
Brickell
P
P
D000818:Animals; D018797:Cell Cycle Proteins; D002642:Chick Embryo; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004330:Drosophila; D029721:Drosophila Proteins; D015723:Gene Library; D017403:In Situ Hybridization; D008957:Models, Genetic; D051788:Myeloid-Lymphoid Leukemia Protein; D009687:Nuclear Proteins / Q000378:metabolism; D011519:Proto-Oncogenes; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015534:Trans-Activators / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D009687:Nuclear Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C063460:Trl protein, Drosophila; C086469:brm protein, Drosophila; D051788:Myeloid-Lymphoid Leukemia Protein