title: A molecular mechanism for the cleavage of a disulfide bond as the primary function of agonist binding to G-protein-coupled receptors based on theoretical calculations supported by experiments.
abstract: A model of the binding site of delta-opioids in the extracellular region of the G-protein-coupled opioid receptor based on modelling studies is presented. The distance between Asp288 and the disulfide bridge (Cys121-Cys198) formed between the first and second extracellular loops was found to be short. This model is consistent with site-directed mutagenesis studies. The arrangement of the ligands found in the receptor led to the development of a reaction mechanism for the cleavage of the disulfide bond catalysed by the ligands. Semi-empirical quantum chemical PM3 and AM1 calculations as well as ab initio studies showed that the interaction between the carboxylic acid side chain of aspartic acid and the disulfide bond leads to the polarization of, and withdrawal of a proton from, the protonated nitrogen of the ligand to one of the sulfur atoms. A mixed sulfenic acid and carboxylic acid anhydrate is formed as an intermediate as well as a thiol. The accompanying cleavage of the disulfide bond may produce a conformational change in the extracellular loops such that the pore formed by the seven-helix bundle opens allowing entrance of the ligand, water and ions into the cell. Cleavage of the disulfide bond after opioid administration was demonstrated experimentally by flow-cytometric measurements employing CMTMR and monobromobimane-based analyses of membrane-located thiols. The suggested mechanism may explain, in a consistent way, the action of agonists and antagonists and is assumed to be common for many G-protein coupled receptors.
authors: Institute of Biochemistry, Martin-Luther-University Halle-Wittenberg, Halle/Saale, Germany.
authors: W
authors: W
authors: Brandt
authors: A
authors: A
authors: Golbraikh
authors: M
authors: M
authors: Täger
authors: U
authors: U
authors: Lendeckel
mesh_terms: D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D019204:GTP-Binding Proteins / Q000378:metabolism*; D006801:Humans; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D008958:Models, Molecular; D015394:Molecular Structure; D009019:Morphinans / Q000737:chemistry / Q000378:metabolism; D009292:Narcotic Antagonists / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D017465:Receptors, Opioid, delta / Q000819:agonists* / Q000737:chemistry / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D004220:Disulfides; D007211:Indoles; D008024:Ligands; D009019:Morphinans; D009292:Narcotic Antagonists; D017465:Receptors, Opioid, delta; C074392:N-methylnaltrindole; D019204:GTP-Binding Proteins; D003545:Cysteine