Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity.
We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K., et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans.
Arita
Y
Y
Graduate School of Medicine, Institute for Molecular and Cellular Biology, Osaka University, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Kihara
S
S
Ouchi
N
N
Takahashi
M
M
Maeda
K
K
Miyagawa
J
J
Hotta
K
K
Shimomura
I
I
Nakamura
T
T
Miyaoka
K
K
Kuriyama
H
H
Nishida
M
M
Yamashita
S
S
Okubo
K
K
Matsubara
K
K
Muraguchi
M
M
Ohmoto
Y
Y
Funahashi
T
T
Matsuzawa
Y
Y
D052242:Adiponectin; D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D015992:Body Mass Index; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D006358:Hot Temperature; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D016014:Linear Models; D008297:Male; D051379:Mice; D008875:Middle Aged; D008970:Molecular Weight; D009765:Obesity / Q000097:blood*; D011485:Protein Binding; D021382:Protein Sorting Signals / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism
D052242:Adiponectin; D000906:Antibodies; D036341:Intercellular Signaling Peptides and Proteins; D021382:Protein Sorting Signals; D011506:Proteins; D011994:Recombinant Proteins