title: Murine 12(R)-lipoxygenase: functional expression, genomic structure and chromosomal localization.
abstract: A cDNA, recently cloned (by Krieg et al. (1998)) from mouse skin, was shown to encode a 12(R)-lipoxygenase. When expressed in HEK cells, the recombinant protein converted methyl arachidonate into the corresponding 12-HETE ester which was shown to be the R-enantiomer by chiral phase chromatography. Neither arachidonic acid nor linoleic acid were substrates for the recombinant protein. The structure of the 12(R)-lipoxygenase gene is unique among all animal lipoxygenases in that it is divided into 15 exons and 14 introns spanning approximately 12.5 kb. By interspecific backcross analysis, the 12(R)-lipoxygenase gene was localized to the central region of mouse chromosome 11.
authors: Research Program on Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany. p.krieg@dkfz-heidelberg.de
authors: P
authors: P
authors: Krieg
authors: M
authors: M
authors: Siebert
authors: A
authors: A
authors: Kinzig
authors: R
authors: R
authors: Bettenhausen
authors: F
authors: F
authors: Marks
authors: G
authors: G
authors: Fürstenberger
mesh_terms: D000818:Animals; D001092:Arachidonate 12-Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics; D016678:Genome; D051379:Mice; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D011994:Recombinant Proteins; D001092:Arachidonate 12-Lipoxygenase