Critical relationship between glycosylation of recombinant lutropin receptor ectodomain and its secretion from baculovirus-infected insect cells.
The lutropin receptor ectodomain overexpressed under the control of the powerful polyhedrin promoter in baculovirus-infected Sf9 insect cells, is mainly found in an inactive, intracellularly-aggregated form. It is secreted in an active form under the control of the P10 promoter, a somewhat weaker and earlier promoter, at the price of a lower production. The apparent molecular masses of the two species encoded by the same cDNA are 48 kDa and 60-68 kDa, respectively. The relationship between the extent and type of glycosylation and the extracellular targeting for the recombinant lutropin receptor ectodomains was investigated precisely with endoglycosidases, lectins of various specificities, and a glycosylation inhibitor, and tested with monoclonal and polyclonal antibodies. The results indicate that the strong polyhedrin promoter probably overwhelms the processing capacity of the ER in Sf9 cells, so that only a high-mannose precursor is expressed in large amounts. Only a minute amount of protein is secreted, which has been processed by Sf9 exoglycosidases/glycosyltransferases and bears complex/hybrid oligosaccharides. The weaker P10 promoter allows secretion of a mature and active receptor ectodomain, bearing complex glycosylation. An important O-linked glycosylation is also added post-translationally on this species. In particular, beta-galactose and sialic acid residues were specifically detected in the secreted species, evidence of the induction of the corresponding glycosyltransferases or of their genes. These results suggest that Sf9 cells should eventually be engineered with chaperones and glycosyltransferases in order to improve the production of demanding glycoproteins such as the porcine lutropin ectodomain, so as to open the way to resolution of the three-dimensional structures of these receptors.
Pajot-Augy
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Unité Récepteurs et Communication Cellulaire, Biologie Cellulaire et Moléculaire, INRA-Biotechnologies, Jouy-en-Josas, France. pajot@biotec.jouy.inra.fr
Bozon
V
V
Remy
J J
JJ
Couture
L
L
Salesse
R
R
D000595:Amino Acid Sequence; D000818:Animals; D016367:Baculoviridae; D001667:Binding, Competitive; D019921:Biotinylation; D002478:Cells, Cultured; D003001:Cloning, Molecular; D004721:Endoplasmic Reticulum / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D006026:Glycoside Hydrolases / Q000378:metabolism; D006031:Glycosylation; D007313:Insecta; D037102:Lectins / Q000378:metabolism; D008969:Molecular Sequence Data; D000077183:Occlusion Body Matrix Proteins; D011134:Polysaccharides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D017510:Protein Folding; D011974:Receptors, LH / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D013552:Swine; D014764:Viral Proteins / Q000235:genetics; D015678:Viral Structural Proteins
D037102:Lectins; D000077183:Occlusion Body Matrix Proteins; D011134:Polysaccharides; D011974:Receptors, LH; D011994:Recombinant Proteins; D014764:Viral Proteins; D015678:Viral Structural Proteins; C009829:polyhedrin protein, Nucleopolyhedrovirus; D006026:Glycoside Hydrolases