Bombesin activates MAP kinase in non-small cell lung cancer cells.
The effects of bombesin (BB) on mitogen activated protein (MAP) kinase were investigated using non-small cell lung cancer (NSCLC) cells. By Western blot, both 42 and 44 kDalton forms of MAP kinase were present in NCI-H1299 and NCI-H838 cells. Addition of BB to NCI-H1299 cells resulted in phosphorylation of the MAP kinase substrate myelin basic protein (MBP). Phosphorylation of MBP was maximal 6 min after the addition of 10 nM BB to NCI-H1299 cells. Addition of gastrin releasing peptide (GRP) or GRP14-27 but not GRP1-16 to NCI-H 1299 cells caused MBP phosphorylation. The effects of BB were inhibited by BW2258U89, a BB receptor antagonist, and PD98059, a MAP kinase kinase inhibitor. Also, PD98059 inhibited the clonal growth of NCI-H1299 cells. These data suggest that MAP kinase may be an important regulatory enzyme in NSCLC.
Koh
S W
SW
Department of Ophthalmology, University of Maryland School of Medicine, Baltimore 21201, USA.
Leyton
J
J
Moody
T W
TW
D000595:Amino Acid Sequence; D001839:Bombesin / Q000378:metabolism*; D017871:Calcium-Calmodulin-Dependent Protein Kinases / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000201:enzymology* / Q000473:pathology; D019886:Gastrin-Releasing Peptide / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology* / Q000473:pathology; D008969:Molecular Sequence Data; D010766:Phosphorylation / Q000187:drug effects; D014407:Tumor Cells, Cultured
D019886:Gastrin-Releasing Peptide; D017871:Calcium-Calmodulin-Dependent Protein Kinases; D001839:Bombesin