Revised primary structures of rat pituitary gamma-lipotrophin and beta-endorphin.
In-depth investigations, by high performance liquid chromatographic purification, radio-immunoassay, mass spectrometry, tandem mass spectrometry, Edman sequencing and limited C-terminal ladder sequencing, were prompted by mass spectrometric charting experiments which suggested that the amino acid sequences for rat gamma-lipotrophin and beta-endorphin require revision. The results for gamma-lipotrophin identify a histidine for glutamine substitution at position 12, and heterogeneity in the expressed protein presumably due to partial dehydration. Partial dehydration for acidic joining peptide, previously reported by Toney et al was corroborated. The results for beta-endorphin confirm the presence of alanine at position 26 and provide no evidence for the expression of multiple forms of the hormone.
Faull
K F
KF
Department of Chemistry Biochemistry, University of California Los Angeles 90095, USA. faull@chem.ucla.edu
Feistner
G J
GJ
Conklin
K
K
Roepstorff
P
P
Andrews
P C
PC
D000595:Amino Acid Sequence; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D005260:Female; D008297:Male; D013058:Mass Spectrometry; D008666:Metalloendopeptidases / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010902:Pituitary Gland / Q000737:chemistry*; D051381:Rats; D020318:Rats, Long-Evans; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D017421:Sequence Analysis; D016339:Spectrometry, Mass, Fast Atom Bombardment; D014357:Trypsin / Q000378:metabolism; D001615:beta-Endorphin / Q000737:chemistry*; D008083:beta-Lipotropin / Q000737:chemistry*
D001615:beta-Endorphin; D008083:beta-Lipotropin; D014357:Trypsin; D008666:Metalloendopeptidases; C039830:peptidyl-Lys metalloendopeptidase