Beta-2 transferrin assay in the identification of perilymph.
OBJECTIVE
Western blot assay for beta-2 transferrin protein is a clinically useful method for the detection of human perilymph and should be used for the diagnosis of perilymph fistulas (PLFs).


BACKGROUND
Considerable controversy exists regarding the diagnosis of PLF. Recent studies suggest that the detection of beta-2 transferrin protein may be useful in the identification of perilymph.


METHODS
To evaluate the usefulness of the beta-2 transferrin assay for identifying human perilymph, paired perilymph samples and negative controls were collected on Gelfoam pledgets from 20 patients who had surgery that opened the inner ear. Blinded immunoelectrophoretic assay (Western blot) for beta-2 transferrin was performed on each specimen.


RESULTS
Only one (5%) of the known perilymph samples and none of the control specimens were definitely positive for beta-2 transferrin. Combined with historical data, this assay has 29% sensitivity, 100% specificity, 100% positive predictive value, and 31% negative predictive value.


CONCLUSIONS
These findings suggest that the beta-2 transferrin protein assay may not be a reliable method for detecting human perilymph when performed using this technique.
Buchman
C A
CA
Department of Otolaryngology, University of Miami School of Medicine, Florida, USA.
Luxford
W M
WM
Hirsch
B E
BE
Fucci
M J
MJ
Kelly
R H
RH
D003052:Cochlear Aqueduct / Q000473:pathology; D005402:Fistula / Q000473:pathology; D006801:Humans; D010498:Perilymph / Q000737:chemistry*; D012189:Retrospective Studies; D014168:Transferrin / Q000032:analysis*
D014168:Transferrin