Metabolism analysis and on-line physiological state diagnosis of acetone-butanol fermentation.
Fermentation equations for acetone-butanol (AB) were applied in a metabolic analysis of the reaction network under various conditions; that is, at different pHs and a high NADH2 turnover rate using methyl viologen, in a Clostridium acetobutylicum culture. The results disclosed variations in the pattern of rate changes that reflected changes in the physiological state. A linear relationship was found to exist between NADH2 generation and butanol production rate. By coupling an automated measurement system with the fermentation model, on-line estimation of the culture state was accomplished. Based on the AB fermentation model, new parameters were defined for on-line diagnosis of the physiological state and determination of the best timing for amplifying NADH2 generation by the addition of methyl viologen to obtain a high level of butanol productivity. A potential means of achieving optimal control for a high level of solvent production, involving the correlation of certain rates, is proposed.
Chauvatcharin
S
S
International Center of Cooperative Research in Biotechnology, Osaka University, 2-1 Yamadaoka, Suitashi, Osaka 565, Japan.
Siripatana
C
C
Seki
T
T
Takagi
M
M
Yoshida
T
T
D000096:Acetone / Q000378:metabolism*; D001431:Bacteriological Techniques; D019149:Bioreactors; D000440:Butanols / Q000378:metabolism*; D003013:Clostridium / Q000254:growth & development* / Q000378:metabolism*; D005285:Fermentation; D013058:Mass Spectrometry; D008838:Microcomputers; D009862:Online Systems
D000440:Butanols; D000096:Acetone