title: Cellular distribution and developmental expression of AMP-activated protein kinase isoforms in mouse central nervous system.
abstract: The mammalian AMP-activated protein kinase is a heterotrimeric serine/threonine protein kinase with multiple isoforms for each subunit (alpha, beta, and gamma) and is activated under conditions of metabolic stress. It is widely expressed in many tissues, including the brain, although its expression pattern throughout the CNS is unknown. We show that brain mRNA levels for the alpha2 and beta2 subunits were increased between embryonic days 10 and 14, whereas expression of alpha1, beta1, and gamma1 subunits was consistent at all ages examined. Immunostaining revealed a mainly neuronal distribution of all isoforms. The alpha2 catalytic subunit was highly expressed in neurons and activated astrocytes, whereas the alpha1 catalytic subunit showed low expression in neuropil. The gamma1 noncatalytic subunit was highly expressed by neurons, but not by astrocytes. Expression of the beta1 and beta2 noncatalytic subunits varied, but some neurons, such as granule cells of olfactory bulb, did not express detectable levels of either beta isoform. Preferential nuclear localization of the alpha2, beta1, and gamma1 subunits suggests new functions of the AMP-activated protein kinase, and the different expression patterns and cellular localization between the two catalytic subunits alpha1 and alpha2 point to different physiological roles.
authors: The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
authors: A M
authors: AM
authors: Turnley
authors: D
authors: D
authors: Stapleton
authors: R J
authors: RJ
authors: Mann
authors: L A
authors: LA
authors: Witters
authors: B E
authors: BE
authors: Kemp
authors: P F
authors: PF
authors: Bartlett
mesh_terms: D000263:Adenylate Kinase / Q000032:analysis / Q000235:genetics* / Q000276:immunology; D000367:Age Factors; D000818:Animals; D000906:Antibodies; D001253:Astrocytes / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology; D002490:Central Nervous System / Q000166:cytology / Q000201:enzymology* / Q000254:growth & development*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D051379:Mice; D008808:Mice, Inbred CBA; D009474:Neurons / Q000201:enzymology; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D000906:Antibodies; D012333:RNA, Messenger; D000263:Adenylate Kinase