Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes.
We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes loss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.
Hernández-Allés
S
S
Departamento de Biología, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
Benedí
V J
VJ
Martínez-Martínez
L
L
Pascual
A
A
Aguilar
A
A
Tomás
J M
JM
Albertí
S
S
D001426:Bacterial Proteins; D002440:Cefoxitin / Q000494:pharmacology; D002513:Cephamycins / Q000494:pharmacology; D004251:DNA Transposable Elements / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000235:genetics; D006801:Humans; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D016254:Mutagenesis, Insertional; D018272:Porins / Q000096:biosynthesis / Q000235:genetics*
D001426:Bacterial Proteins; D002513:Cephamycins; D004251:DNA Transposable Elements; C092233:OmpK36 protein, Klebsiella pneumoniae; D018272:Porins; D002440:Cefoxitin