Isolation of a highly active PSII-LHCII supercomplex from thylakoid membranes by a direct method.
We have developed a simple and novel method to isolate a highly pure and active photosystem (PS) II complex, directly from thylakoid membranes. This complex is a discrete particle and contains all the proteins of the oxygen evolving complex and a set of chlorophyll alb binding proteins. The intactness of both the donor side and the acceptor side has resulted in a very high oxygen evolution activity and therefore offers a superior experimental system to that of PSII enriched membrane fragments in which there is heterogeneity in activities and biochemical composition.
Eshaghi
S
S
Department of Biochemistry, Stockholm University, Sweden.
Andersson
B
B
Barber
J
J
D002462:Cell Membrane / Q000378:metabolism; D002736:Chloroplasts / Q000378:metabolism / Q000648:ultrastructure; D045342:Light-Harvesting Protein Complexes; D008722:Methods; D045322:Photosynthetic Reaction Center Complex Proteins / Q000302:isolation & purification*; D018724:Spinacia oleracea
D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins