Capillary electrophoresis separation of an asparagine containing hexapeptide and its deamidation products.
A capillary electrophoresis (CE) method was developed for the separation of a model hexapeptide (L-Val-L-Tyr-L-Pro-L-Asn-Gly-L-Ala) and its degradation products. Separations using CE had much shorter analysis times than the RP-HPLC assay used previously. CE was also evaluated for possible use in analyzing peptide samples containing polymers. With a thorough rinsing procedure between runs, the presence of a model polymer did not affect the separation of the peptides. The CE assay can be applied to the study of peptide stability in polymers.
Lai
M
M
Department of Pharmaceutical Chemistry, The University of Kansas, Lawrence 66047-2504, USA.
Skanchy
D
D
Stobaugh
J
J
Topp
E
E
D001216:Asparagine / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D003641:Deamination; D019075:Electrophoresis, Capillary / Q000379:methods*; D009842:Oligopeptides / Q000032:analysis / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000032:analysis / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D011108:Polymers / Q000032:analysis; D011142:Polyvinyl Alcohol / Q000737:chemistry
D009842:Oligopeptides; D010446:Peptide Fragments; D010455:Peptides; D011108:Polymers; C089005:valyl-tyrosyl-prolyl-asparaginyl-glycyl-alanine; D001216:Asparagine; D011142:Polyvinyl Alcohol