title: Evaluation of a hybrid artificial liver using a polyurethane foam packed-Bed culture system in dogs.
abstract: OBJECTIVE
  We developed a polyurethane foam packed-bed culture system of hepatocyte spheroids as a hybrid artificial liver (PUF-HAL), which was effective for recovery from liver failure in rat experiments. In this report, the design of a scaled-up PUF-HAL for dogs is described and evaluated using a dog acute liver failure model.
  
  
  METHODS
  Warm ischemic liver failure was induced with a portocaval shunt in each dog. The dogs were divided into two groups: (1) a control group (N = 4), in which each dog was attached to a PUF-HAL without hepatocytes for 9 h, and (2) a HAL group (N = 5), in which each dog was attached to a PUF-HAL with hepatocytes. Blood pressure, blood ammonia, blood glucose, serum creatinine, and other parameters related to liver function were compared between the two groups.
  
  
  RESULTS
  In the HAL group, blood ammonia and serum creatinine levels were significantly lower, and blood pressure and blood glucose levels significantly higher, than those in the control group.
  
  
  CONCLUSIONS
  The scaled-up PUF-HAL developed for large animals is useful as a liver support system in the dog acute liver failure model.
authors: Second Department of Surgery, Kyushu University, Fukuoka, 812-8582, Japan.
authors: T
authors: T
authors: Gion
authors: M
authors: M
authors: Shimada
authors: K
authors: K
authors: Shirabe
authors: K
authors: K
authors: Nakazawa
authors: H
authors: H
authors: Ijima
authors: T
authors: T
authors: Matsushita
authors: K
authors: K
authors: Funatsu
authors: K
authors: K
authors: Sugimachi
mesh_terms: D000641:Ammonia / Q000097:blood; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001794:Blood Pressure; D018929:Cell Culture Techniques / Q000379:methods; D003404:Creatinine / Q000097:blood; D004285:Dogs; D005112:Extracorporeal Circulation; D005260:Female; D008099:Liver / Q000166:cytology*; D017093:Liver Failure / Q000097:blood / Q000503:physiopathology / Q000628:therapy; D019164:Liver, Artificial; D008297:Male; D011140:Polyurethanes / Q000737:chemistry; D016062:Porosity; D018874:Spheroids, Cellular
chemical_list: D001786:Blood Glucose; D011140:Polyurethanes; D000641:Ammonia; D003404:Creatinine