A comparison of the anti-histone and Apop-Tag technique for demonstrating apoptosis with option for silver enhancement.
A novel immunocytochemical method is presented for the qualitative detection of DNA fragmentation in apoptosis. Anti-histone antibody is employed to localize exposed nucleosomal histones (H1, H2a, H2b, H3 and H4) rather than tagging the cut ends of fragmenting DNA as in conventional technique. The method was tested on squamous cell carcinoma of the larynx routinely fixed in formaldehyde and embedded in paraffin wax and compared with results obtained employing Apop-Tag kit (Oncor).
Al-hazzaa
A A
AA
School of Pure and Applied Biology, University of Wales Cardiff, Cardiff, Wales, CF1 3TL, U.K.
Bowen
I D
ID
Birchall
M A
MA
D017209:Apoptosis; D002294:Carcinoma, Squamous Cell / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006657:Histones / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D007822:Laryngeal Neoplasms / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016622:Silver Staining / Q000379:methods*
D006657:Histones