Distribution of gangliosides, GM1 and GM3, in the rat oviduct.
It is known that gangliosides, being ubiquitous membrane components, play important roles in cell-cell recognition, differentiation and transmembrane signalling. GM3, GM1 and GD1a were detected in the rat oviduct as major gangliosides by thin-layer chromatography (TLC) analysis. The total amounts of gangliosides from the oviducts at various times after hormone injection were not much changed. In order to identify their distribution and possible changes during ovulation, frozen sections of the rat oviducts were stained with specific monoclonal antibodies (MAbs) against the ganglio-series gangliosides. GM3 and GM1 were expressed in a different manner, but GD1a and other gangliosides were not immunohistochemically detected. In the ampullar region, GM3 was expressed in all the stroma and epithelial cells, but not GM1. GM1 was also not observed in epithelial cells. Staining by anti-GM1 monoclonal antibodies revealed long and minute thread-like structures in some of the stroma cells, whereas anti-GM3 monoclonal antibodies stained the entire cytoplasm, but not the nucleus, of all the stroma and epithelial cells. Other ganglio-series gangliosides, including GD1a, were not detected to some extent in the ampullar region by immunohistochemistry. Thus, these data suggest that GM3 and GM1 are oviduct-specific gangliosides.
Choo
Y K
YK
Division of Life Science, College of Natural Sciences, Wonkwang University, Iksan, Chunbuk, Korea. ykchoo@wonnms.wonkwang.ac.kr
D000818:Animals; D000911:Antibodies, Monoclonal / Q000187:drug effects; D002725:Chloroform / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D005187:Fallopian Tubes / Q000737:chemistry* / Q000187:drug effects; D005260:Female; D005455:Fluorescent Antibody Technique; D005677:G(M1) Ganglioside / Q000032:analysis* / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000032:analysis* / Q000378:metabolism; D050356:Lipid Metabolism; D000432:Methanol / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D005679:G(M3) Ganglioside; D005677:G(M1) Ganglioside; D002725:Chloroform; D000432:Methanol