An additional exon of stress-inducible heat shock protein 70 gene (HSP70-1).
The HSP70-1 gene, reportedly a single exon, encodes a major stress-inducible 72-kDa heat shock protein (HSP70). We recently demonstrated that patients with major depression had a 162-base pair (bp)-deletion in the 5'-flanking sequence of HSP70-1 mRNA in their peripheral blood mononuclear cells. Present study has been conducted to clarify how this short mRNA is generated, and demonstrated that a novel 385 bp sequence is located 1.1 kb upstream from the HSP70-1 gene of normal subjects. Except for the 162 bp deletion, it is compatible with part of the 5'-flanking sequence of the HSP70-1 gene, and contains another exon of 358 bp (exon 1) that may be connected to the 3'-terminus (exon 3) of the conventional HSP70-1 gene. Alternative transcription of exons 1 and 3 may cause the short mRNA. It is concluded that HSP70-1 gene is constituted of three exons and may cause alternative splicing.
Shimizu
S
S
Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical University School of Medicine, Tokyo, 162-8666, Japan.
Nomura
K
K
Ujihara
M
M
Demura
H
H
D017398:Alternative Splicing / Q000235:genetics; D001483:Base Sequence; D002478:Cells, Cultured; D003001:Cloning, Molecular; D018387:Codon, Initiator / Q000235:genetics; D003863:Depression / Q000235:genetics; D005091:Exons / Q000235:genetics*; D005786:Gene Expression Regulation; D015894:Genome, Human; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D007438:Introns / Q000235:genetics; D007963:Leukocytes, Mononuclear; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D020218:Response Elements / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics; D013312:Stress, Physiological; D014158:Transcription, Genetic / Q000235:genetics*
D018387:Codon, Initiator; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger