Ultrastructural cytochemical study of proteoglycans in the endometrium of pregnant mice using cationic dyes.
Decidualization in rodents is accompanied by remarkable modifications of both fibrillar and non-fibrillar components of the endometrial extracellular matrix. Biochemical studies have shown that the levels of synthesis of hyaluronic acid and sulfated glycosaminoglycans change during decidualization in rodents. As the rodent decidua has regions containing cells in different stages of decidual transformation, we decided to analyse, by an ultrastructural cytochemical technique, the distribution of proteoglycans (PGs) in each region of the decidua of mice on different days of pregnancy. Endometria of mice on days 4, 5 and 7 of pregnancy were processed for electron microscopy in the presence of safranin O, a cationic dye which preserves most of the tissue PGs. The endometrium of non-pregnant mice was used as control. We observed evident differences in the arrangement and distribution of the network of PGs between non-pregnant and 4-day pregnant endometria, as well as between different regions of pregnant endometria. The possible relationship between these modifications and cell transformation that occurs during decidualization is discussed.
Greca
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CP
Department of Cell Biology, Federal University of Paraná, Brazil.
Abrahamsohn
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PA
Zorn
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TM
D000818:Animals; D002412:Cations; D004396:Coloring Agents; D003656:Decidua / Q000737:chemistry* / Q000648:ultrastructure; D004717:Endometrium / Q000737:chemistry* / Q000648:ultrastructure; D005260:Female; D006651:Histocytochemistry; D051379:Mice; D008854:Microscopy, Electron; D010619:Phenazines; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011509:Proteoglycans / Q000032:analysis*; D015203:Reproducibility of Results
D002412:Cations; D004396:Coloring Agents; D010619:Phenazines; D011509:Proteoglycans; C009195:safranine T