Effect of post-induction nutrient feeding strategies on the production of bioadhesive protein in Escherichia coli.
The effect of post-induction nutrient feeding strategies on the production of bioadhesive protein using an IPTG inducible expression system in Escherichia coli was investigated. Cells were cultured in an exponential fed-batch mode to the OD600 of ca. 100 (48 gDCW/L) prior to induction. Six different post-induction nutrient feeding strategies (pH-stat, exponential, constant and linear change in feeding rate with three different slopes) were then applied, and bioadhesive protein production was examined. It was found that post-induction cell growth was independent of nutrient feeding rate. However, bioadhesive protein production was significantly affected by post-induction feeding strategies. Linearly changing post-induction feeding rate with a suitable slope allowed production of bioadhesive protein up to 5.3 g/L, which was higher than that obtained by the other post-induction feeding strategies.
Wong
H H
HH
Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology (KAIST), 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea.
Kim
Y C
YC
Lee
S Y
SY
Chang
H N
HN
D000818:Animals; D001431:Bacteriological Techniques; D019149:Bioreactors; D049872:Bivalvia / Q000235:genetics; D003470:Culture Media; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D005285:Fermentation; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005813:Genes, Synthetic; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D007700:Kinetics; D011498:Protein Precursors / Q000096:biosynthesis* / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000302:isolation & purification
D003470:Culture Media; D011498:Protein Precursors; D011994:Recombinant Proteins; C082021:bioadhesive precursor protein, Mytilus; D007544:Isopropyl Thiogalactoside