First synthesis of a fully active spin-labeled peptide hormone.
For the first time in the electron spin resonance (ESR) and peptide synthesis fields, a fully active spin-labeled peptide hormone was reported. The ESR spectra of this alpha-melanocyte stimulating hormone (alpha-MSH) analogue (acetyl-Toac0-alpha-MSH) where Toac is the paramagnetic amino acid probe 2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid, suggested a pH-independent conformation and a more restricted movement comparatively to the free Toac. Owing to its equivalent biological potency in a skin pigmentation assay as compared to the native alpha-MSH and its unique characteristic (paramagnetic, naturally fluorescent and fully active), this analogue is of great potential for investigation of relevant physiological roles reported for alpha-MSH.
Barbosa
S R
SR
Departamento de Biofisica, Universidade Federal de São Paulo, SP, Brazil.
Cilli
E M
EM
Lamy-Freund
M T
MT
Castrucci
A M
AM
Nakaie
C R
CR
D004578:Electron Spin Resonance Spectroscopy; D011487:Protein Conformation; D013113:Spin Labels; D000521:alpha-MSH / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism
D013113:Spin Labels; D000521:alpha-MSH