Adrenomedullin promotes epithelial restitution of rat and human gastric mucosa in vitro.
We have investigated the effect of adrenomedullin (AM) on restitution of mucosal integrity following damage in rat and human gastric mucosa, measuring the potential difference (PD) on a mucosal strip mounted on an Ussing chamber. Mucosal damage was induced by 0.5, 1.0, and 2.0 M NaCl solution, and it caused an immediate and significant decrease in PD. In the rat AM group, PD recovered significantly more than in control group at 120 min after exposure to 0.5 M (p < 0.01) and 1.0 M (p < 0.05) NaCl solution. In the human AM group, PD completely recovered at 120 min after exposure to 0.5 M (p < 0.05) NaCl solution. In rat mucosa damaged by 0.5 M NaCl solution, the effect was inhibited by human (h)-CGRP(8-37) and there was no significant difference between the h-CGRP(8-37) group and control group. On immunohistochemical examination of rat gastric mucosa, AM was detected within the chief cell. AM probably promotes epithelial restitution primarily through the CGRP receptor, but it does not ameliorate more severe damage of gastric mucosa in vitro.
Fukuda
K
K
Department of Metabolism and Clinical Nutrition, Faculty of Medicine, Kyoto University, Japan. fukuda@metab.kuhp.kyoto-u.ac.jp
Tsukada
H
H
Oya
M
M
Onomura
M
M
Kodama
M
M
Nakamura
H
H
Hosokawa
M
M
Seino
Y
Y
D053607:Adrenomedullin; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology; D003429:Cross Reactions; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D058265:Receptors, Adrenomedullin; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000032:analysis / Q000378:metabolism; D018000:Receptors, Peptide
D008565:Membrane Proteins; D010455:Peptides; D058265:Receptors, Adrenomedullin; D018015:Receptors, Calcitonin Gene-Related Peptide; D018000:Receptors, Peptide; D053607:Adrenomedullin; D015740:Calcitonin Gene-Related Peptide