Cloning of the genomic sequence encoding a processed adenylate kinase 2 pseudogene.
A chromosomal DNA sequence harboring a processed AK2B pseudogene was isolated from a human genomic library. It was a variant of the AK2B gene sequence including several point mutations, deletions, and insertions. The nucleotide sequence of the ORF of the AK2B pseudogene predicted a truncated form of the AK2B mutant suggesting that the processed pseudogene is nonfunctional. A repetitive sequence, AAAAGAGAG, found in the 5' and 3' flanking regions of the pseudogene and the poly(A) tract in the 3' end junction suggest that a mRNA of AK2B may have been converted to the processed pseudogene by retrotransposition events. Previously, it was suggested that an adenylate kinase (AK) 2 related gene on chromosome 2, confirmed by Southern analysis using somatic cell hybrid cell lines, may be a processed pseudogene. It is proposed that the processed pseudogene isolated in this study may be the AK2 related nonfunctional gene localized on human chromosomes 2.
Lee
Y
Y
Molecular and Cellular Biology Research Division, Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon, Korea.
Hong
M
M
Kim
Y J
YJ
Kim
J W
JW
Kim
C H
CH
Lee
K S
KS
Chang
S Y
SY
Lim
J S
JS
Choe
I S
IS
D000263:Adenylate Kinase / Q000235:genetics*; D001483:Base Sequence; D015139:Blotting, Southern; D002874:Chromosome Mapping; D002889:Chromosomes, Human, Pair 2; D003001:Cloning, Molecular; D015698:Genomic Library; D006801:Humans; D007527:Isoenzymes / Q000235:genetics*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D011544:Pseudogenes; D017422:Sequence Analysis, DNA
D007527:Isoenzymes; D000263:Adenylate Kinase; C110938:adenylate kinase 2