Effect of water and enzyme concentration on thermolysin-catalyzed solid-to-solid peptide synthesis.
We have studied a thermolysin-catalyzed solid-to-solid dipeptide synthesis using equimolar amounts of Z-Gln-OH and H-Leu-NH2 as model substrates. The high substrate concentrations make this an effective alternative to enzymatic peptide synthesis in organic solvents. Water content was varied in the range of 0 to 600 mL water per mol substrate and enzyme concentration in the range of 0.5 to 10 g/mol of substrates. High yields around 80% conversion and initial rates from 5 to 20 mmol s-1 kg-1 were achieved. The initial rate increases 10-fold on reducing the water content, to reach a pronounced optimum at 40 mL water per mol substrate. Below this, the rate falls to much lower values in a system with no added water, and to zero in a rigorously dried system. This behavior is discussed in terms of two factors: At higher water contents the system is mass transfer limited (as shown by varying enzyme content), and the diffusion distances required vary. At low water levels, effects reflect the stimulation of the enzymatic activity by water.
Erbeldinger
M
M
Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow G1 1XW, United Kingdom.
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Halling
P J
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D001709:Biotechnology / Q000379:methods; D005973:Glutamine; D007202:Indicators and Reagents; D007700:Kinetics; D007930:Leucine; D010455:Peptides / Q000138:chemical synthesis*; D013820:Thermolysin / Q000378:metabolism*; D014867:Water
D007202:Indicators and Reagents; D010455:Peptides; D014867:Water; D005973:Glutamine; D013820:Thermolysin; D007930:Leucine