Evidence of Trolox and some gallates as synergistic protectors of erythrocytes against peroxyl radicals.
The peroxidation of human erythrocytes induced by peroxyl radical initiator and its inhibition by several gallate esters (e.g., propyl, methyl, ethyl) and Trolox (a more polar analogue of vitamin E) have been studied. The antioxidant activity was determined on erythrocytes against hemolysis generated by a thermal activator, 2,2'-azobis-(2-amidinopropane)dihydrogenchloride. It was found that propyl gallate and its two analogues were more effective than Trolox in preventing cell lysis. However, the combination of gallate esters and Trolox produced a protective effect exceeding the arithmetic sum of their individual contributions. These perceived synergisms occur at more than one level of Trolox at a given level of a gallate ester.
Wu
J
J
Department of Laboratory Medicine and Pathobiology, University of Toronto, ON, Canada.
Sugiyama
H
H
Zeng
L H
LH
Mickle
D
D
Wu
T W
TW
D000975:Antioxidants / Q000494:pharmacology*; D002839:Chromans / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004912:Erythrocytes / Q000378:metabolism*; D005707:Gallic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D011435:Propyl Gallate / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
D000975:Antioxidants; D002839:Chromans; D017382:Reactive Oxygen Species; C048734:ethyl gallate; C052082:methyl gallate; D005707:Gallic Acid; D011435:Propyl Gallate; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid