Role of the S. typhimurium actin-binding protein SipA in bacterial internalization.
Entry of the bacterium Salmonella typhimurium into host cells requires membrane ruffling and rearrangement of the actin cytoskeleton. Here, it is shown that the bacterial protein SipA plays a critical role in this process. SipA binds directly to actin, decreases its critical concentration, and inhibits depolymerization of actin filaments. These activities result in the spatial localization and more pronounced outward extension of the Salmonella-induced membrane ruffles, thereby facilitating bacterial uptake.
Zhou
D
D
Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA.
Mooseker
M S
MS
Galán
J E
JE
D000199:Actins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000942:Antigens, Bacterial / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D001704:Biopolymers; D002462:Cell Membrane / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D008840:Microfilament Proteins; D008856:Microscopy, Fluorescence; D009154:Mutation; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D015398:Signal Transduction; D016596:Vinculin / Q000378:metabolism
D000199:Actins; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001704:Biopolymers; C063664:IpaA protein, Shigella flexneri; D008840:Microfilament Proteins; D011993:Recombinant Fusion Proteins; C118287:SipA protein, Salmonella; D016596:Vinculin