Design of reactive porous polymer supports for high throughput bioreactors: poly(2-vinyl-4,4-dimethylazlactone-co-acrylamide- co-ethylene dimethacrylate) monoliths.
Enzymatic bioreactors with both high flow characteristics and mechanical stability based on macroporous poly(2-vinyl-4, 4-dimethylazlactone-co-acrylamide-co-ethylene dimethacrylate) monoliths have been prepared. Covalent immobilization of trypsin on these support is achieved in a single reaction step using the azlactone functional groups. Optimization of hydrophilic/hydrophobic properties of the monolith affords a support that does not shrink in water and leads to immobilized enzyme that shows high activity in the hydrolysis of both low and high molecular weight substrates such as L-benzoyl arginine ethyl ester and casein. The catalytic activity of the monolithic reactor is maintained even at a flow velocity of 180 cm/min, which substantially exceeds those reported in the literature for packed bed reactors.
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Department of Chemistry, University of California, 718 Latimer Hall, Berkeley, California 94720-1460, USA.
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Fréchet
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D019149:Bioreactors; D001709:Biotechnology; D015195:Drug Design; D004800:Enzymes, Immobilized; D066298:In Vitro Techniques; D007700:Kinetics; D011108:Polymers / Q000737:chemistry; D013379:Substrate Specificity; D014357:Trypsin / Q000378:metabolism
D004800:Enzymes, Immobilized; D011108:Polymers; D014357:Trypsin