title: Neuroprotective effects of Gly-Pro-Glu, the N-terminal tripeptide of IGF-1, in the hippocampus in vitro.
abstract: Insulin-like growth factor 1 (IGF-1) plays a critical role in CNS development. IGF-1 can block neuronal apoptosis in vitro and in vivo. IGF-1 is thought to be cleaved into des-N-(1-3)-IGF-1 and an amino terminal glycine-proline-glutamate (GPE tripeptide). Here we report a neuroprotective role for GPE tripeptide, with enhanced survival of the CA1-2 hippocampal neurons following an excitotoxic insult in vitro. Binding and displacement studies suggest uniquely distributed sites of action within the rat including the hippocampal CA1-2, pyriform cortex, amygdala, choroid plexus, blood vessels and to a lesser extent in the cortical regions. A similar pattern of binding was seen in the human. This finding could lead to new strategies to reduce neuronal death after injury and in disease.
authors: Department of Pharmacology, School of Medicine, University of Auckland, New Zealand.
authors: J
authors: J
authors: Saura
authors: L
authors: L
authors: Curatolo
authors: C E
authors: CE
authors: Williams
authors: S
authors: S
authors: Gatti
authors: L
authors: L
authors: Benatti
authors: C
authors: C
authors: Peeters
authors: J
authors: J
authors: Guan
authors: M
authors: M
authors: Dragunow
authors: C
authors: C
authors: Post
authors: R L
authors: RL
authors: Faull
authors: P D
authors: PD
authors: Gluckman
authors: S J
authors: SJ
authors: Skinner
mesh_terms: D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000737:chemistry*; D009474:Neurons / Q000187:drug effects; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D009842:Oligopeptides / Q000378:metabolism / Q000494:pharmacology*; D009924:Organ Culture Techniques; D051381:Rats
chemical_list: D018696:Neuroprotective Agents; D009842:Oligopeptides; D007334:Insulin-Like Growth Factor I; C062053:glycyl-prolyl-glutamic acid