title: Isolation of pigment-binding early light-inducible proteins from pea.
abstract: The early light-inducible proteins (ELIPs) in chloroplasts possess a high sequence homology with the chlorophyll a/b-binding proteins but differ from those proteins by their substoichiometric and transient appearance. In the present study ELIPs of pea were isolated by a two-step purification strategy: perfusion chromatography in combination with preparative isoelectric focussing. Two heterogeneous populations of ELIPs were obtained after chromatographic separation of solubilized thylakoid membranes using a weak anion exchange column. One of these populations contained ELIPs in a free form providing the first isolation of these proteins. To prove whether the isolated and pure forms of ELIP bind pigments, spectroscopic and chromatographic analysis were performed. Absorption spectra and TLC revealed the presence of chlorophyll a and lutein. Measurements of steady-state fluorescence emission spectra at 77 K exhibited a major peak at 674 nm typical for chlorophyll a bound to the protein matrix. The action spectrum of the fluorescence emission measured at 674 nm showed several peaks originating mainly from chlorophyll a. It is proposed that ELIPs are transient chlorophyll-binding proteins not involved in light-harvesting but functioning as scavengers for chlorophyll molecules during turnover of pigment-binding proteins.
authors: Department of Biochemistry, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden. Iwona@biokemi.su.se
authors: I
authors: I
authors: Adamska
authors: M
authors: M
authors: Roobol-Bóza
authors: M
authors: M
authors: Lindahl
authors: B
authors: B
authors: Andersson
mesh_terms: D029681:Arabidopsis Proteins; D002736:Chloroplasts / Q000737:chemistry; D007525:Isoelectric Focusing; D018532:Pisum sativum / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
chemical_list: D029681:Arabidopsis Proteins; C078410:ELIP protein, Arabidopsis; D010940:Plant Proteins