title: Neurons with choline acetyltransferase immunoreactivity and mRNA are present in the human cerebral cortex.
abstract: We examined the cerebral cortex of five autopsied individuals without neurological and psychiatric diseases by immunohistochemistry using an anti-human recombinant choline acetyltransferase (ChAT) polyclonal antibody and in situ hybridization with 35S-labeled human ChAT riboprobes. The immunohistochemistry detected positive neurons which were medium-sized or large pyramidal neurons located predominantly in layers III and V. The density of such neurons was higher in the motor and secondary sensory areas than in other cortical areas; the immunoreactive neurons in layer V were more densely distributed in the motor area and those in layer III were distributed in the secondary sensory areas. Positively stained, non-pyramidal neurons were observed in the superficial layer of the cingulate gyrus and parahippocampus. No immunoreactive neurons were found in the primary sensory areas. The in situ hybridization detected some neurons with signals for ChAT mRNA in the cerebral cortex, most of which were distributed in layer V of the motor area and in layer III of the secondary visual area. These results indicate that the human cerebral cortex contains cholinergic neurons and displays regional and laminal variations in their distribution.
authors: First Department of Pathology, Faculty of Medicine, Kanazawa University, Ishikawa, Japan.
authors: S
authors: S
authors: Kasashima
authors: A
authors: A
authors: Kawashima
authors: Y
authors: Y
authors: Muroishi
authors: H
authors: H
authors: Futakuchi
authors: I
authors: I
authors: Nakanishi
authors: Y
authors: Y
authors: Oda
mesh_terms: D001344:Autopsy; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000032:analysis* / Q000235:genetics; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D009474:Neurons / Q000201:enzymology*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D002795:Choline O-Acetyltransferase