Effect of salt hydrate pair on lipase-catalyzed regioselective monoacylation of sucrose.
Sucrose monoesters of a fatty acid were synthesized by using lipase in a solvent-free system. When lipase from Mucor miehei was used as a catalyst with capric acid as the donor and sugar as the acceptor, sucrose 6-monocaprate was predominantly produced in a yield of 25.3%. The yield of product was significantly increased by the direct addition of a suitable pair of solid salt hydrates to the reaction mixture to control the water activity (aw). Among the salt hydrate pairs investigated, the barium hydroxide, 8/1H2O pair resulted in the highest yield of the product. This salt addition method was also successfully employed for acylation of primary hydroxyl groups in various unprotected mono- and disaccharides such as glucose, galactose, fructose, trehalose, mannose, maltose, and lactose.
Kim
J E
JE
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon 305-701, Korea.
Han
J J
JJ
Yoon
J H
JH
Rhee
J S
JS
D000215:Acylation; D019149:Bioreactors; D001709:Biotechnology; D004951:Esterification; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D009089:Mucor / Q000201:enzymology; D012492:Salts; D012997:Solvents; D013395:Sucrose / Q000378:metabolism*
D012492:Salts; D012997:Solvents; D013395:Sucrose; D008049:Lipase