The response of cultured bone cells to resorbable polyglycolic acid and silicone membranes for use in orbital floor fracture repair.
Two membranes intended for use in repairing fractures of the orbital floor--reinforced silicone and biodegradable polyglycolic acid (PGA)--were evaluated in vitro using a rat bone cell culture model. After two weeks in culture, cells had colonised the surface of both materials. Bone cells penetrated the weave of the PGA membrane after three weeks in culture, forming a calcified collagenous bone-like tissue within the weave of the PGA at the same time as there was evidence of resorption of the PGA. In contrast, cells could easily be dislodged from the surface of the reinforced silicone membrane and there was less evidence of mineralised extra cellular matrix production. The production of a bone-like tissue within the weave of the PGA membrane supported previous reports of osteoconductive activity of this material.
Hatton
P V
PV
Biomaterials Research Group, School of Clinical Dentistry, University of Sheffield, UK.
Walsh
J
J
Brook
I M
IM
D000818:Animals; D001672:Biocompatible Materials; D001673:Biodegradation, Environmental; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009917:Orbital Fractures / Q000601:surgery*; D016348:Osseointegration; D011100:Polyglycolic Acid; D051381:Rats; D012826:Silicone Elastomers; D013997:Time Factors