Qualitative membrane enzyme immunoassay for detection of cytomegalovirus antibody.
A new qualitative membrane immunoassay for use in detecting IgG antibodies to cytomegalovirus (CMV) was compared with a standard microplate enzyme immunoassay (EIA). A total of 179 patients were tested by the EIA for IgG- and IgM-specific antibodies to CMV and then compared with the new membrane immunoassay. Among the 179 sera, five specimens were repeatably invalid.  Among the 174 evaluable sera, 128 (73.6%) were positive for IgG antibody to CMV by the EIA method.  Of these 128 sera, the Transtat-CMV was positive in 124 (96.9%).  The remaining 46 sera were negative for IgG antibodies by both methods. The Transtat-CMV membrane immunoassay appears to be a reliable and easily used method not requiring additional equipment or extended incubation times.  As a qualitative-only assay, it cannot be used for diagnosis.
Baptist Regional Laboratories, Department of Pathology, University of Tennessee, Memphis.
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DL
Smalley
D L
DL
Demchuk
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AP
Webster
D003587:Cytomegalovirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D012680:Sensitivity and Specificity