Heparin washout in the pediatric Cell Saver bowl.
The possibility of residual heparin in washed red cells transfused to neonatal or pediatric cardiac patients following bypass prompted a measurement of heparin concentrations.  Samples were taken during 10 adult and 10 neonatal and pediatric bypass cases.  Sample A was from the bypass circuit, Sample B from the Haemonetics Cell Saver bowl inlet before washing, Sample C from the Cell Saver bowl outlet after washing, and Sample D from the patient ten minutes after protamine.  Heparin concentrations were measured by a chromogenic assay using activated Factor X.  There was no significant difference between the adult and pediatric groups in the levels of heparin concentration on bypass, pre-washing and post-washing, and in the patients following protamine. In the pediatric group, only .002% of the pre-washed heparin remained after washing.  This extremely low level of heparin (.0027 units/ml) is only 0.34 units in a 125 ml pediatric unit of Cell Saver blood.  Based on post bypass patient samples, this has no clinical significance. Therefore, the Cell Saver can be used safely with neonates and pediatric patients without concern regarding residual heparin when properly processed.
Sistino
J J
JJ
Columbia-Presbyterian Medical Center, N.Y. 10032.
Owitz
D
D
Mongero
L B
LB
D001804:Blood Transfusion, Autologous / Q000295:instrumentation*; D002315:Cardiopulmonary Bypass; D002648:Child; D004867:Equipment Design; D005069:Evaluation Studies as Topic; D006493:Heparin / Q000032:analysis*; D006801:Humans; D007231:Infant, Newborn