A technique for brain temperature control during ischemia, suitable for measurements with ion-sensitive microelectrodes.
A technique is described for maintaining rat brain temperature constant during ischemia, a technique that also allows measurements with, and calibration of, ion-sensitive microelectrodes under defined temperature conditions.  The brain temperature is controlled by a stream of air of defined temperature and humidity, which is perfused through a box enclosing the animal's head.  A device for calibration of ion-sensitive microelectrodes is temperature controlled by similar principles.  The air stream is delivered by a heater/humidifier that is standard in many commercial respirators/ventilators.  When the relative humidity of the air stream is greater than 98%, the neocortical temperature can be maintained within less than 0.5 degrees C during 15 min of ischemia. The biological applicability of the technique is discussed.
Laboratory for Experimental Brain Research, Lund University Hospital, Sweden.
A
A
Ekholm
B K
BK
Siesjö
D000818:Animals; D001831:Body Temperature / Q000502:physiology*; D002545:Brain Ischemia / Q000517:prevention & control* / Q000628:therapy; D006863:Hydrogen-Ion Concentration; D008297:Male; D008839:Microelectrodes; D051381:Rats; D017208:Rats, Wistar