Immunoassay techniques for the detection of circulating antibodies to collagen following the use of collagen medical devices.
During the use of collagen medical devices, some adverse clinical reactions occur involving both the cellular and humoral types of the immune response.  Thus, the development of immunoassay techniques for measuring the presence and the levels of circulating anticollagen antibodies is required.  The authors present their protocol:  it is a solid phase radioimmunoassay using collagen coated on polystyrene microplates and labelled protein A as the tracer. An example of the application of the technique is described. Anticollagen antibodies were monitored in 586 patients undergoing bovine collagen implant therapy.  In a retrospective study on 166 patients we found a good correlation between the presence of antibodies to collagen and cellular immune reactions such as a positive skin test or adverse clinical reactions after implantation. A prospective study on 420 patients showed that the pretreatment anticollagen serologic test can be useful as an adjunct to skin testing in the conservative management of patients desiring bovine collagen implant therapy.  The use of this double test allowed avoidance of any major clinical reaction and reduced minor signs of intolerance.
Centre de Radioanalyse, Lyon, France.
D J
DJ
Hartmann
G
G
Charriere
G
G
Ville
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001672:Biocompatible Materials / Q000009:adverse effects; D002417:Cattle; D003094:Collagen / Q000009:adverse effects*; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D005260:Female; D006801:Humans; D007111:Immunity, Cellular; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D011863:Radioimmunoassay / Q000379:methods; D012189:Retrospective Studies; D012882:Skin Tests / Q000379:methods