Bone marrow processing on the Haemonetics V50 cell separator.
We have processed 27 bone marrow (BM) harvests using the Haemonetics V50 cell separator with a paediatric plasmapheresis set and programmed for lymphocyte collection.  The mean starting volume of 843 mL was processed in 6-8 cycles to a buffy coat (BC) with a mean volume of 230 mL.  The mean starting mononuclear cell (MNC) count was 1.22 x 10 8/kg recipient weight, and recovery was 92%.  Clonogenic potential of the BC was assessed using CFU-GM assays and recovery was measured after cryopreservation or purging.  On 4 occasions where major ABO incompatibility existed between donor and recipient, both BM and BC were consecutively diluted in compatible blood and processed twice.  This achieved a calculated reduction in donor erythrocytes of 98%.  The procedure was efficient and yielded a BC fraction suitable for cryopreservation and purging.  Adequate stem-cells were retained as verified by CFU-GM assays and documentation of stable engraftment.
South Western Regional Transfusion Centre, Bristol, U.K.
N A
NA
Anderson
J M
JM
Cornish
V
V
Godwin
M J
MJ
Gunstone
A
A
Oakhill
D H
DH
Pamphilon
D000293:Adolescent; D000328:Adult; D001781:Blood Component Removal / Q000295:instrumentation / Q000379:methods; D001853:Bone Marrow; D016026:Bone Marrow Transplantation / Q000295:instrumentation / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation*; D002648:Child; D002675:Child, Preschool; D015925:Cryopreservation; D005069:Evaluation Studies as Topic; D006801:Humans; D007223:Infant; D008875:Middle Aged