Response of canine bone to a synthetic bone graft material.
A model simulating a spiral diaphyseal fracture with butterfly fragments and bone loss was utilized to evaluate an hydroxyapatite/tricalcium phosphate, and collagen composite bone graft substitute in twelve dogs.  The resultant grafted and contralateral control femora were tested in torsion at one year.  This study examines the histological response to the graft material as well as crack propagation and fracture surface morphology using light microscopy and SEM.  SEM and gross evaluation of the grafted bones revealed that 8/12 had fractured through bone outside the osteotomy site and all fractures included bone outside the graft site.  No graft material was demonstrated at the points of initiation or termination of fracture for any of the bones.  It was apparent that recorticalization had begun to occur at the graft site but the canal had not yet fully formed.  The HA/TCP was seen to be tightly bound in tissue which had the appearance of new bone.  Bone was found to be in direct apposition to the surface of the ceramic and within pores with no intervening soft tissue.  Much of the new bone had remodeled into well organized Haversian systems with some patchy areas of woven bone and osteoid seen with polarized light illumination.
University of Mississippi Medical Center, Department of Orthopaedic Surgery, Jackson 39216.
K R
KR
St John
L D
LD
Zardiackas
R J
RJ
Black
R
R
Armstrong
D000818:Animals; D016723:Bone Remodeling; D016025:Bone Transplantation / Q000379:methods*; D002130:Calcium Phosphates; D003094:Collagen; D004195:Disease Models, Animal; D004285:Dogs; D005264:Femoral Fractures / Q000601:surgery*; D006652:Histological Techniques; D006882:Hydroxyapatites; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D010027:Osteotomy; D010784:Photomicrography; D013314:Stress, Mechanical