New whole blood methods and instruments:  glucose measurement and test menus for critical care.
A glucose oxidase-H 2O 2 substrate-selective electrode (SSE) along with eight other electrodes allows the Stat Profile 5 (SP5) to measure glucose, electrolytes, blood gases, pH, and hematocrit simultaneously in whole blood.  For heparinized blood gas syringe samples (n=178), orthogonal (Deming) regression showed y=0.43 + 1.00x (mmol/L), where y is whole blood glucose, and x is plasma glucose measured with the Ektachem 400 glucose oxidase slide method.  For heparinized plasma (n=197), y=0.25 + 1.02x.  Least squares linear regression showed y=0.25 + 1.00x (s yxx=0.49) for whole blood versus plasma, y = 0.30 + 1.01x (s yxx=0.49) for plasma versus plasma, and r=0.994 for both cases.  Whole blood and plasma glucose in the same samples measured by SSE differed by less than 1%.  The SP5 combines the largest number of whole blood tests currently available in a rapid response instrument well-suited for care of the critically ill patient.
Kost
G J
GJ
School of Medicine, University of California, Davis 95616.
Wiese
D A
DA
Bowen
T P
TP
D001774:Blood Chemical Analysis / Q000295:instrumentation*; D001786:Blood Glucose / Q000032:analysis*; D003422:Critical Care; D004867:Equipment Design; D006400:Hematocrit; D006801:Humans; D016014:Linear Models; D010949:Plasma; D012044:Regression Analysis