Simultaneous measurement of VMA and HVA in 24-hour urine samples.
A simple method is described for the simultaneous measurement of vanillylmandelic acid (VMA) and homovanillic acid (HVA) in 24-hour urine samples based upon isocratic high-performance liquid chromatography with electrochemical detection (HPLC-ECD). VMA was measured using VMA-SKREEN kits (Biochemical Diagnostics), a microcolumn diazo method and HPLC-ECD test method.  HVA was measured using Bio-Rad HVA HPLC reagent kits (Bio-Rad Laboratories) and the test method. No significant statistical difference was found in mean or variance when this method was compared with the micro-column diazo method for VMA and HPLC-ECD for HVA using the commercial anion-exchange kit.  The linear regression equation for VMA was ŷ = 0.92 x + 0.21, and for HVA was ŷ = 1.10 x + 0.06.  The Pearson correlation coefficient for VMA was r = 0.896 and for HVA was r = 0.996. This method yields results for 24-hour urinary VMA and HVA that compare well with established commercial methods.
Department of Medical Technology, Bowling Green State University, OH 43403.
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D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004563:Electrochemistry; D006719:Homovanillic Acid / Q000652:urine*; D006801:Humans; D007231:Infant, Newborn; D009447:Neuroblastoma / Q000175:diagnosis*; D010673:Pheochromocytoma / Q000175:diagnosis*; D011933:Reagent Kits, Diagnostic; D012044:Regression Analysis; D015203:Reproducibility of Results; D014642:Vanilmandelic Acid / Q000652:urine*