Leukocyte-depleted platelets prepared from pooled buffy coat post-transfusion increment and "in vitro bleeding time" using the Thrombostat 4000/2.
A technique for preparation of platelet concentrate (PC) from pooled buffy coat (BC) is described in this study.  The yield of platelets from 4 BCs was 310+/-48x10 9 per unit with a leukocyte content of 21+/-20x10 6 per unit.  When a leukocyte removing filter was interconnected, Sepacell 5N and Pall PL-50 filters, no leukocytes could be found in 56 of 59 and in 36 of 37 PCs, with a maximum content of 2.3 and 0.54x10 6 leukocytes per PC, respectively.  The platelet yield was 276+/-37 and 279+/-54x10 9 per unit, respectively.  The in vitro bleeding time (IVBT), using the Thrombostat 4000/2, corresponded with the corrected count increment in most of 11 thrombocytopenic patients receiving platelet transfusions.  However, in two transfusions no correction of IVBT was obtained.  This technique offers a new possibility to investigate the functional capacity of transfused platelets.
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
C F
CF
Högman
L
L
Eriksson
J
J
Kristensen
D001760:Bleeding Time; D001781:Blood Component Removal / Q000295:instrumentation* / Q000379:methods; D016913:Blood Component Transfusion / Q000295:instrumentation / Q000379:methods; D001792:Blood Platelets; D005374:Filtration; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods; D010976:Platelet Count / Q000295:instrumentation / Q000379:methods