Leucocyte depletion of neocyte-rich red blood cell concentrates by use of immunomagnetic beads.
In a series of pilot experiments we depleted neocyte-rich red blood cell concentrates of contaminating leucocytes by use of immunomagnetic beads. The beads were coated with monoclonal antibodies directed against the leucocyte common antigen CD 45.  The number of cells isolated depended on the dose of beads which also corresponded to the decrease in leucocytes in the neocyte concentrates.  The required ratio of beads to target cells was approximately 25:1, the depletion rate of leucocytes was about 96.0-99.8%.
Blutspendezentrale des Städtischen Klinikums, Karlsruhe, Germany.
B
B
Häcker-Shahin
D J
DJ
Giannitsis
D000740:Anemia / Q000628:therapy*; D000911:Antibodies, Monoclonal / Q000627:therapeutic use; D016913:Blood Component Transfusion / Q000379:methods; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000382:microbiology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000627:therapeutic use; D008212:Lymphocyte Depletion / Q000379:methods*; D011137:Polystyrenes / Q000627:therapeutic use*