An attempt to prepare hepatitis B virus (HBV)-free plasma by ultrafiltration using microporous regenerated cellulose hollow fiber.
Hepatitis B virus (HBV) can be effectively removed from HBV-positive plasma by filtration with a Bemberg Microporous Membrane (BMM) with a pore size of 30 nm or less, however considerable amounts of macromolecular IgM and Factor VIII are trapped in the BMM.  We report that HBV-free plasma with adequate amounts of all of the plasma proteins can be obtained by double filtration with a BMM with a port size of 50 nm. Thus it may be possible to remove HBV or other transfusion-associated viruses from plasma by BMM filtration with good recovery of all of the plasma components.
Hokkaido Red Cross Blood Center, Sapporo, Japan
S
S
Sekiguchi
K
K
Ito
M
M
Kobayashi
H
H
Ikeda
S
S
Manabe
T
T
Tsurumi
G
G
Ishikawa
M
M
Satani
K
K
Yamaguchi
D001798:Blood Proteins / Q000032:analysis; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006525:Hepatitis, Viral, Human / Q000517:prevention & control / Q000635:transmission; D006801:Humans; D008851:Micropore Filters; D010949:Plasma; D014462:Ultrafiltration / Q000295:instrumentation* / Q000379:methods