Biocompatibility evaluation of glass ionomer cement using cell culture techniques.
Cell culture techniques have been employed to carry out a preliminary investigation into the biocompatibility of a glass ionomer cement (GIC).  A modification of the commonly used Agar Overlay test and a rapid, colorimetric assay based on the reduction of tetrazolium salt were used.  The GIC is shown to leach a cytotoxic agent, possibly fluoride.  This agent is effectively removed by an extraction procedure, and re-examination of the GIC demonstrates its cytocompatibility.
Institute of Medical and Dental Bioengineering, University of Liverpool, UK.
P J
PJ
Doherty
D000362:Agar; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003124:Colorimetry / Q000379:methods; D005459:Fluorides / Q000633:toxicity; D005899:Glass Ionomer Cements / Q000737:chemistry; D008422:Materials Testing / Q000379:methods