Bioluminescence-enhanced immunoassays: an ultrasensitive method.
Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins are illustrated in this paper with the enzyme immunological determination of total IgE in human serum.  The usable range of standard curves is from 5 pg to 5000 pg per liter.  The intra- and interassay coefficients of variation of this test are in the same range as obtained for chromogenic enzyme immunoassays.
Geiger
R
R
Medor GmbH, Herrsching, Germany.
Miska
W
W
Kuss
P
P
D000937:Antigen-Antibody Reactions; D001920:Bradykinin / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D049454:Firefly Luciferin / Q000737:chemistry; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis* / Q000737:chemistry; D008163:Luminescent Measurements; D012680:Sensitivity and Specificity