title: Collection of peripheral blood stem cells (PBSC) after chemotherapy and administration of rhGM-CSF in children weighing less than 17 kg.
abstract: Peripheral blood stem cells (PBSC) were collected from six children weighing less than 17 kg. Stem cell pools had been expanded by chemotherapy and rhGM-CSF. Nineteen procedures were performed with a continuous-flow separator (CS 3000 plus) using central venous access. The extracorporeal line was primed with red blood cells and 5% albumin (HA). Acid-citrate-dextrose:- formulae A(ACD-A) was added in a median ratio 1:11 (range 1:9-1:15). A median number of 5.6 x 10(8) kg-1 NC (3.5-9.7) and 7.1 x 10(6) kg-1 CD34+ cells (2.1-26.1) were collected per patient. Four patients were transplanted with PBSC and showed normal haematopoietic recovery (leucocytes > 1000 microL-1 between days 11 and 12). The results show that successful PBSC collection and transplantation is possible even in small paediatric patients. Administration of growth factors results in a marked increase of peripheral white blood cells and in a higher yield of NC and CD34+ cells in PBSC collections.
authors.affiliation: Department of Transfusion Medicine, University Hospital Vienna, Austria.
authors.forename: N
authors.initials: N
authors.lastname: Worel
authors.forename: C
authors.initials: C
authors.lastname: Peteres
authors.forename: K
authors.initials: K
authors.lastname: Gerhartl
authors.forename: A
authors.initials: A
authors.lastname: Wagner
authors.forename: S
authors.initials: S
authors.lastname: Jurko
authors.forename: P
authors.initials: P
authors.lastname: Höcker
mesh_terms: D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001835:Body Weight; D002675:Child, Preschool; D003131:Combined Modality Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007937:Leukapheresis; D009369:Neoplasms / Q000097:blood / Q000628:therapy*; D011994:Recombinant Proteins / Q000627:therapeutic use; D014182:Transplantation, Autologous