title: Flow cytometry related to red cells.
abstract: Researchers in Transfusion Medicine Science have benefited from the use of the flow cytofluorometer. The flow cytometer has distinct advantages over visual examination of antigen-antibody reactions. The flow cytometer measures fluorescence per cell, and through the use of anti-IgG tagged with a fluorochrome, cells with differing levels of cell-bound IgG can be quantitated. This has been used in the study of allo- and autoantibodies. Immunohematologists, with the wide range of red cell alloantibodies in many blood group systems, have a seemingly unending supply of materials to enable studies of red cells. This article describes the published reports involving flow cytometry related to red cells. Four areas are discussed: detection of red cell-bound IgG, detection of red cell immunogobulins other than IgG, detection and quantitation of red cell antigens, and detection and quantitation of red cell populations.
authors.affiliation: Technical Services, American Red Cross Blood Services, Penn-Jersey Region, Philadelphia, PA 19123, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Nance
mesh_terms: D004906:Erythrocyte Count; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood