Studies on IgA class circulatory red cell antibodies--I. Preparation and identification of IgA class anti-A,anti-B.
To elucidate the role of IgA class alloantibodies in transfusion practice, anti-A,anti-B was prepared using pooled serum from healthy group O donors. IgG was removed by protein A and protein G, whilst IgM was extracted by concanavalin A and anti-human mu chain. The final preparation contained both IgA1 and IgA2; it had an IgA concentration similar to the original serum pool and an environment as near to normal as possible; the absence of IgG and IgM was confirmed by sensitive enzyme-linked antiglobulin tests, and extensive haemagglutination studies showed that anti-A,anti-B was the only red cell antibody present.
Booth
J R
JR
Trent Blood Transfusion Centre, Sheffield, U.K.
Sokol
R J
RJ
D000017:ABO Blood-Group System / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007518:Isoantibodies / Q000097:blood* / Q000145:classification