title: Pathology techniques.
abstract: Pathology techniques developed over the past decade may be successfully applied to the diagnosis of orbital disease. Tissue specimens may be immunophenotyped by immunohistochemical staining and flow cytometry. Immunohistochemical staining provides a qualitative analysis of localization of antigenic determinants and flow cytometry provides a quantitative analysis. The molecular biologic technique of polymerase chain reaction enables detection of minute amounts of material by amplification from DNA primers. Fine-needle aspiration biopsy is an alternative to open biopsy in select cases. The most important concept regarding these techniques is communication with the ophthalmic pathologist regarding the collection and interpretation of the specimen.
authors.affiliation: L.F. Montgomery Eye Pathology Laboratory, Emory Eye Center, Atlanta, GA 30322, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Diaz
authors.forename: H E
authors.initials: HE
authors.lastname: Grossniklaus
mesh_terms: D003941:Diagnostic Techniques, Ophthalmological; D005128:Eye Diseases / Q000473:pathology*; D006801:Humans; D010338:Pathology, Clinical / Q000379:methods*; D015203:Reproducibility of Results