Immunoglobulin and circulating immune complex kinetics during immunoadsorption onto protein A sepharose.
Immunoadsorption onto protein A sepharose can be applied to eliminate immunoglobulins, autoantibodies and circulating immune complexes from the circulation. In vivo kinetic studies showed that all IgG subclasses are removed from the patient's plasma although IgG3 elimination is variable and dependent on the presence of other immunoglobulins. IgG elimination half time was 4.8 days during intermittent and 2.9 days for daily therapy in the absence of immunosuppression. Autoantibodies and immune complexes can be cleared effectively but administration of intravenous immunoglobulins should be avoided because of competition for protein A binding sites. Redistribution/ denovo synthesis (half time 2.1-8.8 d for IgG 1-4) occurred in the absence of immunosuppression.
Braun
N
N
Medizinischen Klinik und Poliklinik, Abteilung III, Tübingen, Germany. nbraun@uni-tuebingen.de
Gutenberger
S
S
Erley
C M
CM
Risler
T
T
D000936:Antigen-Antibody Complex / Q000097:blood*; D016022:Case-Control Studies; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000628:therapy*; D012685:Sepharose / Q000031:analogs & derivatives*; D013205:Staphylococcal Protein A