Improved lectin agglutination method for T-cell depletion of HLA-mismatched bone marrow grafts in children.
For T-cell depletion in HLA-nonidentical bone marrow transplantation of children with malignant diseases, we improved the original lectin/rosetting method described in 1981 by adding anti-CD2/3 coated donor red blood cells to the combination to achieve lectin agglutination in one step. Further improvements in handling led to a shortened and simplified method and better quality of the graft. Five bone marrow grafts prepared with this modified protocol contained a median number of 6 (0-28) x 10(4) T-cells per kg, corresponding to 0.02 (0-0.08)% CD3+ cells and 6 (3.7-10.5) x 10(6) CD34+ cells per kg at a median body-weight of 7 (5-38)kg. The overall recoveries after T-cell depletion were: NC 17 (10-44)%, CD34+ cells 61 (22-100)%, and CFU-GM 55 (29-212)%.
Schreiner
T
T
Department of Transfusion Medicine and Red Cross Blood Center, Ulm, Germany.
Wiesneth
M
M
Slaper-Cortenbach
I
I
Maccari
B
B
Erne
E
E
Bischof
C
C
Müller
S
S
Friedrich
W
W
Kubanek
B
B
D000371:Agglutination; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002648:Child; D002675:Child, Preschool; D006086:Graft vs Host Disease / Q000517:prevention & control; D006650:Histocompatibility Testing; D006801:Humans; D037102:Lectins; D008212:Lymphocyte Depletion / Q000379:methods*; D014184:Transplantation, Homologous