title: The effect of ion implantation on cellular adhesion.
abstract: As there are only a finite number of materials suitable for orthopaedic reconstruction, considerable effort has been devoted recently to investigating ways of altering the surface chemistry of prosthetic materials without altering their bulk properties.  Ion beam implantation is one such technique which is appropriate for orthopaedic reconstructive materials.  This paper investigates the early effect of ion beam modification on cellular attachment of bone derived cells using a prototype device which measures the strength of attachment of individual cells to a silicon substratum.  The results point to several conclusions. (1) There is no evidence that ion beam implantation with nitrogen, phosphorus, manganese or magnesium produces increased adhesion of human bone derived cells. (2) Surface etching with hydrofluoric acid, electron bombardment and thermal oxidation increases the strength of attachment between cells and substrata. (3) There is a correlation between wettability and rate of cellular attachment to oxygen implanted substrata during the first 2 h after cellular seeding.  However, the increase in cellular attachment cannot be entirely explained by the change in critical surface tension or via increased fibronectin attachment to the substrata.
authors.affiliation: Bone and Biomaterial Research Unit, School of Pathology, University of New South Wales, Australia.
authors.forename: C R
authors.initials: CR
authors.lastname: Howlett
authors.forename: M D
authors.initials: MD
authors.lastname: Evans
authors.forename: K L
authors.initials: KL
authors.lastname: Wildish
authors.forename: J C
authors.initials: JC
authors.lastname: Kelly
authors.forename: L R
authors.initials: LR
authors.lastname: Fisher
authors.forename: G W
authors.initials: GW
authors.lastname: Francis
authors.forename: D J
authors.initials: DJ
authors.lastname: Best
mesh_terms: D016025:Bone Transplantation / Q000295:instrumentation* / Q000379:methods; D002448:Cell Adhesion / Q000502:physiology; D005069:Evaluation Studies as Topic; D005353:Fibronectins; D006801:Humans; D006858:Hydrofluoric Acid; D007477:Ions; D008274:Magnesium / Q000737:chemistry; D008345:Manganese / Q000737:chemistry; D008422:Materials Testing; D009584:Nitrogen / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D019736:Prostheses and Implants; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties