Studies on IgA class circulatory red cell antibodies--II. Properties of IgA class anti-A,anti-B.
The properties of IgA class red cell alloantibodies were determined using an anti-A,anti-B preparation confirmed to be free from IgG and IgM. The following characteristics were found: a thermal optimum of 37 degrees C; saline agglutination enhanced by anti-human IgA; activity substantially unchanged after 1 year's storage at 4 degrees C, but reduced by papain and destroyed by pepsin and by reduction and alkylation; neutralization by AB blood group substance; an inability to activate complement; and a distinctive pattern of apparent molecular sizes (reflecting dimeric and monomeric forms and complexes with serum proteins) which was significantly altered by the action of enzymes and by reduction and alkylation.
Booth
J R
JR
Trent Blood Transfusion Centre, Sheffield, U.K.
Sokol
R J
RJ
D000017:ABO Blood-Group System / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood*; D007518:Isoantibodies / Q000097:blood* / Q000145:classification