Aspects of platelet storage.
The proper storage of platelets requires sufficient knowledge of the behaviour of these cells, made by nature to react instantly. It is important not to activate them during preparation and storage and to maintain oxidative metabolism at the highest possible degree. Optimally, the citrate concentration in the storage medium should be 8-10 mmol/L. The addition of acetate either to the anticoagulant or to a platelet additive solution gives the potential for improved platelet storage. In order to evaluate the clinical efficiency of platelet concentrates (PCs), corrected count increment is to be recommended for frequent use. The in vitro bleeding time seems to be a valuable supplement, both for research and clinical purposes. Bacterial contamination is a threat which can be diminished by using an appropriate technique for preparation and storage. Rapid automated bacterial culture makes it possible to detect contamination which may be particularly important if the shelf life of PCs is being extended beyond the present 5 days.
Högman
C F
CF
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
D000925:Anticoagulants / Q000494:pharmacology; D001769:Blood / Q000382:microbiology; D001792:Blood Platelets / Q000187:drug effects; D001793:Blood Preservation; D006801:Humans