Collection of peripheral blood stem cells (PBSC) after chemotherapy and administration of rhGM-CSF in children weighing less than 17 kg.
Peripheral blood stem cells (PBSC) were collected from six children weighing less than 17 kg. Stem cell pools had been expanded by chemotherapy and rhGM-CSF. Nineteen procedures were performed with a continuous-flow separator (CS 3000 plus) using central venous access. The extracorporeal line was primed with red blood cells and 5% albumin (HA). Acid-citrate-dextrose:- formulae A(ACD-A) was added in a median ratio 1:11 (range 1:9-1:15). A median number of 5.6 x 10(8) kg-1 NC (3.5-9.7) and 7.1 x 10(6) kg-1 CD34+ cells (2.1-26.1) were collected per patient. Four patients were transplanted with PBSC and showed normal haematopoietic recovery (leucocytes > 1000 microL-1 between days 11 and 12). The results show that successful PBSC collection and transplantation is possible even in small paediatric patients. Administration of growth factors results in a marked increase of peripheral white blood cells and in a higher yield of NC and CD34+ cells in PBSC collections.
Worel
N
N
Department of Transfusion Medicine, University Hospital Vienna, Austria.
Peteres
C
C
Gerhartl
K
K
Wagner
A
A
Jurko
S
S
Höcker
P
P
D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001835:Body Weight; D002675:Child, Preschool; D003131:Combined Modality Therapy; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000627:therapeutic use*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007937:Leukapheresis; D009369:Neoplasms / Q000097:blood / Q000628:therapy*; D011994:Recombinant Proteins / Q000627:therapeutic use; D014182:Transplantation, Autologous