title: Cytokines in platelet concentrates: a comparison of apheresis platelet (haemonetics) and filtered and unfiltered pooled buffy-coat derived platelet concentrates.
abstract: Variable degrees of platelet activation, shape changes, microvesiculation and fragmentation may occur during collection, processing and storage of platelet concentrates (PCs), contributing to different rate of platelet storage lesion. Leukocytes contribute to both the frequency of transfusion reactions and the acceleration of the rate of platelet storage lesion hence leukocyte removal of platelet concentrates has been introduced to overcome these problems. However transfusion reaction can still occur with the use of leuko-reduced products and it is not fully elucidated that the rate of storage lesion is equivalent for filtered and unfiltered counter parts. This issue has been addressed in this manuscript comparing the generation of cytokines during storage in PCs derived from pooled buffy coat with the standard apheresis products, with a similar level of leukocyte contamination. The EDTA-induced shape change in platelet was used as an index of platelet functional integrity. In addition IL-8 and TGF beta were used as indicators of filtration process-inducing stimulation of cytokines. Our results clearly indicate that a rapid disc/spheric conversion occurs during storage of buffy-coat derived PC, and while prestorage filtration reduces both IL-8 content immediately after filtration and at the end of platelet shelf life but such a process may lead to slight enhancement of the rate of TGF beta generation indicating that any additional process may have some bearing in stimulation of TGF beta release.
authors.affiliation: North London Blood Transfusion Centre, Colindale, London, UK.
authors.forename: M J
authors.initials: MJ
authors.lastname: Seghatchian
authors.forename: M
authors.initials: M
authors.lastname: Wadhwa
authors.forename: R
authors.initials: R
authors.lastname: Thorpe
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D016207:Cytokines / Q000032:analysis*; D006801:Humans; D017713:Platelet Transfusion; D010983:Plateletpheresis