Comparison of performance of six different cell separators in collecting peripheral blood mononuclear cells.
We compared the efficacy of six different cell separators in collecting peripheral mononuclear cells to be used for autologous or homologous peripheral stem cell transplantation. The product obtained with the Dideco Vivacell cell separator showed a low percentage of mononuclear cells (38%) in the final product and a high platelet efficiency (38%). The Baxter CS3000 Plus cell separator required the longest time to load and prime the kit (18 min), it showed a high MNC efficiency (68%), with the highest percentage of MNC in the final product, the highest platelet efficiency (45%), a low red blood cell contamination in the final product (2.7 mL), the highest extracorporeal volume (450 mL) and a high percentage of technical failures (15%). The product obtained with the Fresenius AS104 cell separator with P1Y kit showed the highest final volume (297 mL), the lowest platelet efficiency (12%) and the lowest extracorporeal volume (230 mL). The same cell separator with C4Y kit showed a lower MNC efficiency (52 vs 60%) and a higher percentage of MNC in final product (63 vs 41%). The platelet contamination in final product was the lowest (18 x 10(9)/100 mL). The Haemonetics MCS3p cell separator required the lowest time to load and prime the kit (5 min), it showed the highest MNC efficiency (71%). The blood volume processed per hour (1328 mL) and the percentage of MNC in final product was lowest (32%), the extracorporeal volume (450 mL) was the highest. The Cobe Spectra cell separator allowed to process the highest blood volume per hour (3383 mL) and the final product had the lowest red blood cell contamination (2.3 mL/100 mL). The Dideco Excel cell separator required the longest time to load and prime the kit (18 min), the lowest MNC efficiency (38%), the highest platelet contamination in final product. Furthermore this machine showed the highest percentage of technical failure (20%). None of the six instruments have all the required preconditions and the ideal cell separator for peripheral stem cell apheresis at present is not available on the market.
Bellavita
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P
Department of Immunohematology and Blood Transfusion Centre, Ospedali Riuniti di Bergamo, Italy.
Celega
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E
Poma
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R
D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D019650:Hematopoietic Stem Cell Mobilization / Q000295:instrumentation*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008875:Middle Aged; D009369:Neoplasms / Q000628:therapy*; D014182:Transplantation, Autologous; D014184:Transplantation, Homologous