Annexin V, a new marker of platelet storage lesion: correlation with dMPV.
Released annexin V, an intracellular platelets glycoprotein, was used to determine the cellular injury which occurred during storage of platelet concentrates. Twenty-eight units of leuco-reduced apheresis platelet concentrates, obtained without leucocyte filtration, were analysed. Released annexin V showed a significant correlation with EDTA-induced shape changes of platelet (r = 0.62, P < 0.01) while poor correlation was found between released annexin V and pH or MPV. The combination of released annexin V with dMPV provides excellent markers of the platelet storage lesion for quality monitoring, based on morphological/functional integrities and cellular injury, which are of direct relevance to clinical efficacy of platelet concentrates.
Krailadsiri
P
P
National Blood Service-North London, UK.
Seghatchian
J
J
Amiral
J
J
Vissac
A M
AM
Contreras
M
M
D017304:Annexin A5; D015415:Biomarkers; D001792:Blood Platelets / Q000502:physiology*; D001793:Blood Preservation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010976:Platelet Count; D017713:Platelet Transfusion