Immunosorbent for IgG apheresis: an in vitro study.
We obtained anti-IgG Sepharose-an immunosorbent with sheep monospecific polyclonal antibodies against human IgG-for extracorporeal removal of human IgG. Appropriate conditions for effective usage of the immunosorbent were chosen. Binding capacity and selectivity of anti-IgG Sepharose were determined and compared with the corresponding properties of other sorbents for IgG apheresis; these properties appeared to be acceptable for immunoapheresis procedures. Methods for therapeutic application of anti-IgG Sepharose is expected to be effective when used in immunoapheresis procedures for treatment of autoimmune diseases and for preparation of patients for organ transplantation.
Kiseleva
E A
EA
Institute of Experimental Cardiology of Cardiology Research Center, Moscow, Russia.
Afanasieva
O I
OI
Kosheleva
N A
NA
Pokrovsky
S N
SN
D001781:Blood Component Removal; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007164:Immunosorbents