[Comparison of two methods for the study of the in vitro susceptibility to sertaconazole of yeast clinical isolates].
We evaluated a commercial method for studying the in vitro susceptibility to sertaconazole based on its diffusion in agar with standardized tablets, with the aim of determining its correlation with the method of microdilution in Shadomy modified liquid medium (YNBg). A total of 110 Candida genus strains (50 C. albicans, 26 C. tropicalis, 15 C. glabrata, 8 C. parapsilosis, 8 C. krusei, 2 C. guilliermondii and 1 C. kefyr) from pathological clinical processes were used. The results of both techniques showed a statistically significant correlation that depended on the type of reading used in the liquid medium microdilution technique, with results being -0. 4199 with IC50; -5135 with IC90: -0.6634 with MIC24 h; and -0.4945 with MIC48 h. These values demonstrate the existence of a correlation for sertaconazole, and that it is bigger when it is compared with the logarithm of the MIC obtained after 24 hours of incubation.
Carrillo-Muñoz
A J
AJ
Departamento de Microbiología, ACIA, C/Valle de Ordesa no. 4, 08031 Barcelona.
Tur-Tur
C
C
Hernández-Molina
J M
JM
D000935:Antifungal Agents / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects*; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D013876:Thiophenes / Q000494:pharmacology*
D000935:Antifungal Agents; D007093:Imidazoles; D013876:Thiophenes; C061131:sertaconazole