Enzyme-linked immunosorbent assay for apolipoproteins A-I and B in the urine of patients with nephrotic syndrome.
Enzyme-linked immunosorbent assay (ELISA) has been used for measuring apolipoproteins A-I and B in the urine. ApoB is absent in urine of healthy subjects, and apoA-I is determined in trace quantity. In patients with chronic glomerulonephritis quantity of apoA-I in urine was 117 times as much as in control group. ApoB is present in urine of patients in considerable quantity (1528 +/- 315 micrograms/l). The ELISA method for determining apoA-I and apoB in urine makes it possible to evaluate the gravity of pathological process in kidney.
Poliakov
L M
LM
Institute of Biochemistry, Siberian Branch of RAMS, Novosibirsk, Russia.
Poteriaeva
O N
ON
Panin
L E
LE
D000328:Adult; D000368:Aged; D016632:Apolipoprotein A-I / Q000652:urine*; D001055:Apolipoproteins B / Q000652:urine*; D015415:Biomarkers; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008875:Middle Aged; D009404:Nephrotic Syndrome / Q000652:urine*
D016632:Apolipoprotein A-I; D001055:Apolipoproteins B; D015415:Biomarkers