title: Serum antibody response of Castellana sheep to Haemonchus contortus infection and challenge: relationship to abomasal worm burdens.
abstract: Primary and secondary serum antibody responses to Haemonchus contortus were studied in Castellana sheep. Ten-month-old sheep were infected (200 L3/kg live weight (lw)) and challenged (400 L3/kg lw) or uninfected and equally challenged with H. contortus. Primary infections induced a partially protective response upon challenge, characterized by higher serum protein levels, longer prepatent periods, lower fecal egg counts, and significant reduction in the establishment rate of the parasite and abomasal adult and L4 worm burdens. The resistant status of the infected and challenged sheep was not clearly related either to the serum specific antibody levels (IgG: IgG1, IgG2; IgM; IgA) estimated by ELISA or to immunodetection patterns in the Western blots.
authors: Departamento de Patología Animal I, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain.
authors: M T
authors: MT
authors: Gómez-Muñoz
authors: M
authors: M
authors: Cuquerella
authors: L A
authors: LA
authors: Gómez-Iglesias
authors: S
authors: S
authors: Méndez
authors: F J
authors: FJ
authors: Fernández-Pérez
authors: C
authors: C
authors: de la Fuente
authors: J M
authors: JM
authors: Alunda
mesh_terms: D000018:Abomasum / Q000469:parasitology*; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D000947:Antigens, Helminth / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D015153:Blotting, Western / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005243:Feces / Q000469:parasitology; D005260:Female; D006188:Haemonchiasis / Q000276:immunology / Q000662:veterinary*; D006189:Haemonchus / Q000276:immunology*; D008297:Male; D010270:Parasite Egg Count / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001798:Blood Proteins