title: Limbic seizures increase pronociceptin mRNA levels in the thalamic reticular nucleus.
abstract: We studied pronociceptin gene expression following limbic seizures. Northern blot analysis revealed increased pronociceptin mRNA levels in the thalamus (but not in the hippocampus) 3-24 h after kainate administration, with maximal effect (2-fold increase over basal levels) reached at 6 h. No variation in pronociceptin mRNA levels was observed 1-6 h after a stimulus-evoked kindled seizure. Carrageenan failed to affect pronociceptin gene expression in the thalamus, indicating that pain and/or acute stress do not account for kainate effects. In situ hybridization revealed that kainate evokes a dramatic (4-fold) increase in pronociceptin mRNA levels over the thalamic reticular nucleus. Kindled seizures evoked only a small, non-significant increase in pronociceptin gene expression over the dentate gyrus of the hippocampus.
authors: Department of Clinical and Experimental Medicine and Biotechnology Centre, University of Ferrara, Italy.
authors: G
authors: G
authors: Bregola
authors: S
authors: S
authors: Candeletti
authors: P
authors: P
authors: Romualdi
authors: M
authors: M
authors: Simonato
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D017403:In Situ Hybridization; D007608:Kainic Acid; D007696:Kindling, Neurologic / Q000502:physiology; D008032:Limbic System / Q000503:physiopathology*; D008297:Male; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011957:Receptors, Opioid / Q000235:genetics*; D012640:Seizures / Q000139:chemically induced / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology*; D013787:Thalamic Nuclei / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D011957:Receptors, Opioid; C119224:pronociceptin; D007608:Kainic Acid