[Cytotoxic effect of energy supply system inhibitors on cells from Ehrlich ascite carcinoma].
It was shown that inhibitors of oxidative phosphorylation (cyanide, rotenone, and oligomycin) and very low concentrations of exogenous prooxidants exerted a pronounced cytotoxic effect on Ehrlich ascites carcinoma cells. We propose that cell injury by reactive oxygen forms is the cause of the cytotoxic effect of the studied inhibitors. It was shown via flow cytometry that inhibitors of oxidative phosphorylation and exogenous prooxidants block cell progress in the cell cycle and induce appearance of cells with reduced DNA content.
Teplova
V V
VV
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Russia.
Makarov
P R
PR
Kudriavtsev
A A
AA
Lezhnev
E I
EI
Evtodienko
Iu V
IuV
D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D003864:Depression, Chemical; D006019:Glycolysis; D051379:Mice; D010085:Oxidative Phosphorylation; D014407:Tumor Cells, Cultured
D000255:Adenosine Triphosphate