Bordetella pertussis waaA encodes a monofunctional 2-keto-3-deoxy-D-manno-octulosonic acid transferase that can complement an Escherichia coli waaA mutation.
Bordetella pertussis lipopolysaccharide (LPS) contains a single 2-keto-3-deoxy-D-manno-octulosonic acid (Kdo) residue, whereas LPS from Escherichia coli contains at least two. Here we report that B. pertussis waaA encodes an enzyme capable of transferring only a single Kdo during the biosynthesis of LPS and that this activity is sufficient to complement an E. coli waaA mutation.
Isobe
T
T
The Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OES, United Kingdom.
White
K A
KA
Allen
A G
AG
Peacock
M
M
Raetz
C R
CR
Maskell
D J
DJ
D001886:Bordetella pertussis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D016695:Glycosyltransferases / Q000235:genetics; D008070:Lipopolysaccharides / Q000302:isolation & purification; D009154:Mutation; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics; D013400:Sugar Acids / Q000378:metabolism; D013696:Temperature; D014166:Transferases / Q000235:genetics* / Q000378:metabolism
D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D014166:Transferases; D016695:Glycosyltransferases; C019917:3-deoxy-D-manno-octulosonate transferase; C080934:lipooligosaccharide 1,5-heptosyltransferase