Identification and analysis of the promoter region of the human NeuroD-related factor (NDRF)1.
We isolated and characterized the human NeuroD-related factor (NDRF)/NeuroD2/KW8 gene. The NDRF gene consisted of two exons and one intron. NDRF had one transcriptional starting point, and in its 5'-flanking region, no TATA box was detected, but 16 E boxes (CANNTG) were present. RNA blotting analysis revealed that HIT-T15 and D283 cells expressed a 3.3 kb band of the NDRF transcript. Promoter analysis by luciferase assay demonstrated that luciferase activity changed between -120 and -195, and between -451 and -564, both regions wherein a single E box existed. Radiation hybrid mapping showed that NDRF linked the marker SHGC-36242 with a LOD score of 8.53 and was located on 17q12-22.
Kitamura
T
T
Third Department of Internal Medicine, Hiroshima University, School of Medicine, 1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8551, Japan.
Miyachi
T
T
Nakamura
S
S
Kawakami
H
H
D000818:Animals; D001483:Base Sequence; D051792:Basic Helix-Loop-Helix Proteins; D002460:Cell Line; D018257:Helix-Loop-Helix Motifs / Q000235:genetics; D006801:Humans; D008126:Lod Score; D008156:Luciferases; D008969:Molecular Sequence Data; D009479:Neuropeptides / Q000235:genetics*; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000302:isolation & purification; D015183:Restriction Mapping; D014407:Tumor Cells, Cultured
D051792:Basic Helix-Loop-Helix Proteins; D008156:Luciferases; D009479:Neuropeptides; D012333:RNA, Messenger; C490925:NEUROD2 protein, human