Analysis of human lymphotropic T-cell virus type II-like particle production by recombinant baculovirus-infected insect cells.
The molecular processes involved in retrovirus assembly and budding formation remain poorly understood. The gag-pro-pol genes of human lymphotropic T-cell virus type II (HTLV-II) are translated into Gag, Gag-Pro, or Gag-Pro-Pol by frameshift events. In the present study, we investigated the roles of the gag, pro, and pol regions of HTLV-II in viral particle formation using recombinant baculoviruses. In this study we could successfully produce mature HTLV-II viral particles containing core structures using a construct expressing the entire gag-pro-pol region. We also investigated the role of the pol region in particle formation. Deletion of the pol region affects viral particle assembly or release very little, indicating that the gag-pro region is sufficient for viral particle formation and maturation. Expression of the Gag proteins alone or Gag proteins with inactivated viral proteases (Pro) resulted in the formation of viral particles; however, these particles did not contain core structures. These results suggest the intracellular expression of Gag with Pro of HTLV-II is essential for the production of mature virus particles, whereas that of Pol is not.
Takahashi
R H
RH
Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo, 162, Japan. htakahas@nih.go.jp
Nagashima
K
K
Kurata
T
T
Takahashi
H
H
D000818:Animals; D002460:Cell Line; D015750:Genes, gag; D015751:Genes, pol; D005822:Genetic Vectors; D015367:Human T-lymphotropic virus 2 / Q000502:physiology*; D006801:Humans; D009036:Moths; D016296:Mutagenesis; D017924:Nucleopolyhedroviruses; D011995:Recombination, Genetic; D015682:Retroviridae Proteins, Oncogenic / Q000096:biosynthesis; D014771:Virion; D019065:Virus Assembly
D015682:Retroviridae Proteins, Oncogenic; C047061:internal protein p24, Human T-lymphotropic virus 2