Absorbability of calcium from a new calcium supplement prepared from bovine marrow-free bone in rats.
Powdered bovine marrow-free bone was completely solubilized with lactic and citric acids under reduced pressure. The resulting solution was lyophilized to obtain a stable powder form (total bone extract, TBE), and the calcium (Ca) absorbability of TBE from intestine was investigated in normal rats. Each animal perorally received 10 mg of Ca in 1 mL of distilled water as extrinsic 45Ca-labeled TBE, intrinsic 45Ca-labeled Ca lactate, or intrinsic 45Ca-labeled Ca carbonate. The amount of radioactivity in plasma was measured periodically up to 34 h after dosing, and pharmacokinetic parameters were calculated from the radioactivity in plasma. The time taken to reach the maximal 45Ca level (Tmax) did not differ among the three groups. The area under the plasma 45Ca level/time curve (AUC infinity) and the radioactivity at Tmax (Cmax) values for the TBE group were significantly higher than those of the Ca carbonate group. Similar results were observed between the Ca lactate and the Ca carbonate groups. No significant difference was observed in the AUC infinity and the Cmax values between the TBE and the Ca lactate groups. Radioactivity in a femur 34 h after dosing was highest in the Ca lactate group and lowest in the Ca carbonate group among the three groups. Both the TBE and the Ca lactate groups showed significant higher whole-body 45Ca retention than the Ca carbonate group did, although no significant difference was found between the TBE and the Ca lactate groups. These findings indicate that the Ca absorbability of TBE is almost comparable with that of Ca lactate and higher than that of Ca carbonate. Therefore TBE would be useful as a Ca supplement with relatively high absorbability from intestine.
Ohtani
M
M
Research and Development Center, Sangi Co. Ltd., Osaka, Japan.
Tsugawa
N
N
Kamao
M
M
Okano
T
T
D000818:Animals; D001842:Bone and Bones / Q000737:chemistry*; D002118:Calcium / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D002119:Calcium Carbonate / Q000008:administration & dosage / Q000493:pharmacokinetics; D017610:Calcium Compounds / Q000008:administration & dosage / Q000493:pharmacokinetics; D002132:Calcium Radioisotopes; D002417:Cattle; D019343:Citric Acid; D019587:Dietary Supplements; D005612:Freeze Drying; D007408:Intestinal Absorption; D007700:Kinetics; D007773:Lactates / Q000008:administration & dosage / Q000493:pharmacokinetics; D019344:Lactic Acid; D008297:Male; D011312:Pressure; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D014020:Tissue Extracts / Q000008:administration & dosage*
D017610:Calcium Compounds; D002132:Calcium Radioisotopes; D007773:Lactates; D014020:Tissue Extracts; D019343:Citric Acid; C110051:calcium lactate; D019344:Lactic Acid; D002119:Calcium Carbonate; D002118:Calcium