Cloning and expression of bovine herpesvirus-1 glycoprotein C.
Glycoprotein C (gC) of Bovine Herpesvirus-1 (BHV-1) is expressed at high levels on surface of infected cells and on virus envelope. It is relatively immunodominant in antibody response to BHV-1 infection and protective in immunized bovines against BHV-1 challenge. In an attempt to express gC in mammalian cells, the 2.4 kb BamHI-EcoRI fragment, containing complete coding sequence of the gC gene was excised from a recombinant plasmid and cloned under the control of RSV-LTR. The resultant plasmid pRSV-gC was transfected into MDBK cells and expression of gC was detected by indirect immunofluorescence. The non-permeabilized cells revealed surface expression of gC.
Gupta
P K
PK
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
Saini
M
M
Gupta
L K
LK
Bandyopadhyay
S K
SK
Garg
S K
SK
D000818:Animals; D015139:Blotting, Southern; D002417:Cattle; D002460:Cell Line; D003001:Cloning, Molecular; D007242:Herpesvirus 1, Bovine / Q000235:genetics*; D007150:Immunohistochemistry; D014162:Transfection; D014759:Viral Envelope Proteins / Q000096:biosynthesis / Q000235:genetics*
D014759:Viral Envelope Proteins; C038535:glycoprotein gC, herpes simplex virus type 1