Abscisic acid-induced secretory proteins in suspension-cultured cells of winter wheat.
In suspension-cultured cells of winter wheat (Triticum aestivum L. cv. Chihokukomugi), the accumulation of soluble secretory proteins in the culture medium was promoted by ABA treatment in comparison with non-treated cells. The total amount of secretory proteins in ABA-treated cells was 1.7-fold higher than that in non-treated cells. The analysis of two-dimensional electrophoresis revealed that at least twelve secretory proteins were induced by ABA, and these were named WAS (wheat ABA-induced secretory) proteins 1 to 12. N-terminal amino acid sequence analysis of WAS proteins revealed the sequences of WAS-2 and WAS-3. Homology searches showed that WAS-2 had 55% identity with the N-terminus of the wheat chemically induced gene (WCI-5 gene) product. WAS-3 was also shown to have 93% identity with the N-terminus of the barley protein R, a typical member of thaumatin-like proteins (TLPs). Immunoblot analysis also suggested that WAS-3 was related to protein R. These results suggest that exogenous ABA induces some basic secretory proteins that are related to the plant defense system in wheat.
Kuwabara
C
C
Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan.
Arakawa
K
K
Yoshida
S
S
D000040:Abscisic Acid / Q000494:pharmacology*; D000595:Amino Acid Sequence; D018929:Cell Culture Techniques / Q000379:methods; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D015151:Immunoblotting; D008969:Molecular Sequence Data; D037121:Plant Lectins; D010940:Plant Proteins / Q000276:immunology / Q000378:metabolism*; D013549:Sweetening Agents; D014908:Triticum / Q000378:metabolism
D003470:Culture Media; D037121:Plant Lectins; D010940:Plant Proteins; D013549:Sweetening Agents; D003208:Concanavalin A; C003427:thaumatin protein, plant; D000040:Abscisic Acid