Sequence-independent assembly of spermatid mRNAs into messenger ribonucleoprotein particles.
During mammalian spermatogenesis, meiosis is followed by a brief period of high transcriptional activity. At this time a large amount of mRNA is stored as messenger ribonucleoprotein (mRNP) particles. All subsequent processes of sperm maturation occur in the complete absence of transcription, primarily using proteins which are newly synthesized from these stored mRNAs. By expressing transgene mRNAs in the early haploid spermatids of mice, we have investigated the sequence requirements for determining whether specific mRNAs in these cells will be stored as mRNP particles or be assembled into polysomes. The results suggest that mRNAs which are transcribed in spermatids are assembled into mRNP particles by a mechanism that acts independently of mRNA sequence. Our findings reveal a fundamental similarity between the mechanisms of translational control used in spermatogenesis and oogenesis.
Schmidt
E E
EE
Howard Hughes Medical Institute, Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah 84112, USA. eschmidt@howard.genetics.utah.edu
Hanson
E S
ES
Capecchi
M R
MR
D000818:Animals; D002460:Cell Line; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000235:genetics; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000378:metabolism; D011132:Polyribosomes / Q000235:genetics; D011479:Protamines / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics*; D013087:Spermatids / Q000378:metabolism*; D013091:Spermatogenesis / Q000235:genetics*; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D019076:Transgenes / Q000235:genetics
C513176:Prm1 protein, mouse; D011479:Protamines; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases