Diamine-plasma treated and Cu(II)-incorporated poly(hydroxyethylmethacrylate) microbeads for albumin adsorption.
Poly(2-hydroxyethylmethacrylate) (PHEMA) microbeads prepared by suspension polymerization were treated with diamine-plasmas (i.e. ethylene-diamine (EDA) and hexamethylene diamine (HMDA)) in a glow-discharge reactor in which the exposure time and glow-discharge power were changed between 5 and 30 min and 5 and 20 W, respectively. The amount of nitrogen deposition increased both with increase in exposure time and glow-discharge power. The maximum amounts of nitrogen deposition on the microbeads were 22.3 and 23.4 micromol g(-1) with the EDA- and HMDA-plasmas. Then, Cu(II) ions were incorporated onto the PHEMA microbeads by chelating with the nitrogen-carrying functional groups. Different amounts of Cu(II) ions (2.4-6.8 mg g(-1)) were incorporated on the PHEMA microbeads by changing the initial concentration of Cu(II) ions. Bovine serum albumin (BSA) adsorption onto the unmodified PHEMA, diamine-plasma treated PHEMA, and diamine-plasma treated Cu(II)-incorporated PHEMA microbeads was investigated. The non-specific adsorption of BSA on the unmodified microbeads was very low (0.22 mg BSA g(-1)). Deposition of nitrogen increased the BSA adsorption (9.3 mg g(-1) for EDA-plasma and 12.7 mg g(-1) for HMDA-plasma). Cu(II)-incorporation significantly increased the BSA adsorption (154 mg g(-1) for EDA-plasma and 178 mg g(-1) for HMDA-plasma). Further increases in the albumin adsorption capacities of the polymer microbeads (185 mg g(-1) for EDA-plasma and 208 mg g(-1) for HMDA-plasma) were observed when human plasma was used. More than 92% of the adsorbed albumin molecules was desorbed in 1 h in the desorption medium containing 0.5 M NaSCN at pH 8.0. Repeated adsorption-desorption cycles showed the feasibility of these plasma-modified polymer microbeads.
Denizli
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A
Department of Chemistry, Hacettepe University, Ankara, Turkey.
Denizli
F
F
Pişkin
E
E
D000327:Adsorption; D000818:Animals; D019149:Bioreactors; D001769:Blood / Q000378:metabolism*; D002417:Cattle; D003300:Copper / Q000378:metabolism* / Q000493:pharmacokinetics; D003959:Diamines / Q000494:pharmacology; D005029:Ethylenediamines / Q000494:pharmacology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008863:Microspheres; D010949:Plasma / Q000187:drug effects / Q000378:metabolism; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D012709:Serum Albumin / Q000493:pharmacokinetics*; D012710:Serum Albumin, Bovine / Q000493:pharmacokinetics
D003959:Diamines; D005029:Ethylenediamines; D012709:Serum Albumin; D011102:Polyhydroxyethyl Methacrylate; D012710:Serum Albumin, Bovine; C031234:ethylenediamine; D003300:Copper; C007297:1,6-diaminohexane