title: Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism.
abstract: Overpressured layer chromatography was combined with the highly sensitive and rapid digital autoradiography (DAR) and mass spectrometry to separate, detect, and identify 3H- and 14C-labeled deramciclane metabolites in different biological matrixes. Several minor and major metabolites were separated from plasma and urine samples. The radioactive metabolites localized by DAR were scraped from the thin-layer chromatographic plate and transferred to a mass spectrometer for structure identification. Several metabolites were isolated and characterized, including hydroxy-N-desmethyl deramciclane, which is described in detail. The combination of techniques is efficient and has good sensitivity: about 2 micrograms metabolite from a biological matrix was isolated and identified this way.
authors: Institute of Chemistry of the Hungarian Academy of Sciences, Budapest, Hungary.
authors: K
authors: K
authors: Ludányi
authors: K
authors: K
authors: Vékey
authors: J
authors: J
authors: Szúnyog
authors: E
authors: E
authors: Mincsovics
authors: T
authors: T
authors: Karancsi
authors: K
authors: K
authors: Ujszászy
authors: K B
authors: KB
authors: Nemes
authors: I
authors: I
authors: Klebovich
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000493:pharmacokinetics*; D001345:Autoradiography / Q000379:methods*; D001892:Camphanes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002250:Carbon Radioisotopes; D002845:Chromatography / Q000379:methods*; D004285:Dogs; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000493:pharmacokinetics*; D014316:Tritium
chemical_list: D014151:Anti-Anxiety Agents; D001892:Camphanes; D002250:Carbon Radioisotopes; D012702:Serotonin Antagonists; D014316:Tritium; C060555:deramciclane