Role of L-selectin in physiological manifestations after burn and smoke inhalation injury in sheep.
The effects of a monoclonal antibody against L-selectin [leukocyte adhesion molecule (LAM)1-3] on microvascular fluid flux were determined in conscious sheep subjected to a combined injury of 40% third-degree burn and smoke inhalation. This combined injury induced a rapid increase in systemic prefemoral lymph flow (sQlymph) from the burned area and a delayed-onset increase in lung lymph flow. The initial increase in sQlymph was associated with an elevation of the lymph-to-plasma oncotic pressure ratio; consequently, it leads to a predominant increase in the systemic soft tissue permeability index (sPI). In an untreated control group, the increased sPI was sustained beyond 24 h after injury. Pretreatment with LAM1-3 resulted in earlier recovery from the increased sPI, although the initial responses in sQlymph and sPI were identical to those in the nontreatment group. The delayed-onset lung permeability changes were significantly attenuated by pretreatment with LAM1-3. These findings indicate that both leukocyte-dependent and -independent mechanisms are involved in the pathogenesis that occurs after combined injury with burn and smoke inhalation.
Sakurai
H
H
Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical College, Tokyo, Japan.
Schmalstieg
F C
FC
Traber
L D
LD
Hawkins
H K
HK
Traber
D L
DL
D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001794:Blood Pressure; D002056:Burns / Q000503:physiopathology*; D002302:Cardiac Output; D005260:Female; D006439:Hemodynamics; D019041:L-Selectin / Q000276:immunology / Q000502:physiology*; D008196:Lymph / Q000502:physiology; D008833:Microcirculation / Q000503:physiopathology*; D010100:Oxygen / Q000097:blood; D011652:Pulmonary Circulation / Q000502:physiology*; D012756:Sheep; D015208:Smoke Inhalation Injury / Q000503:physiopathology*; D013997:Time Factors
D000911:Antibodies, Monoclonal; D019041:L-Selectin; D010100:Oxygen