Effects of mercury on the isolated heart muscle are prevented by DTT and cysteine.
The protective effects of dithiothreitol (DTT, 50 microM) and cysteine (CYS, 100 microM) against toxic effects of HgCl2 (1, 2.5, 5, and 10 microM) were studied in isolated, isometrically contracting rat papillary muscles. Force reduction promoted by Hg2+ was prevented by both DTT and CYS. Also, after both treatments, no significant changes in dF/dt were observed. A progressive reduction in the time to peak tension was observed when increased concentrations of HgCl2 were used after CYS and DTT treatment. This was an indication that the enhancement of calcium release from the sarcoplasmic reticulum produced by mercury was not affected by DTT and CYS. Tetanic contractions were also studied. After treatment with DTT or CYS tetanic tension did not change. No significant reduction of tetanic tension was observed during treatment with 1 microM Hg2+ but its reduction was observed after 5 microM Hg2+. Myosin ATPase activity was also affect by Hg2+, being completely blocked by 1 microM Hg2+ and reduced by 50% with 0.15 microM Hg2+. Full activity was restored by using 500 nM DTT. These findings suggest that several but not all toxic effects of Hg2+ on the mechanical activity of the heart muscle are prevented by protectors of SH groups such as DTT and CYS. The enhancement of the Ca2+ release from the sarcoplasmic reticulum by Hg2+ during activation was not affected by prior treatment with DTT and CYS, suggesting that interactions with SH groups may not be important for the activation of the Ca2+ channel of the sarcoplasmic reticulum.
Vassallo
D V
DV
Department of Physiological Sciences, Federal University of Espirito Santo, Vitoria, ES, Brazil.
Moreira
C M
CM
Oliveira
E M
EM
Bertollo
D M
DM
Veloso
T C
TC
D000818:Animals; D003545:Cysteine / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D066298:In Vitro Techniques; D007537:Isometric Contraction / Q000187:drug effects; D008628:Mercury / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D009200:Myocardial Contraction / Q000187:drug effects; D009206:Myocardium / Q000201:enzymology; D009218:Myosins / Q000378:metabolism; D010210:Papillary Muscles / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D013439:Sulfhydryl Reagents; D009218:Myosins; D008628:Mercury; D003545:Cysteine; D004229:Dithiothreitol