title: Application of cybernetic models to metabolic engineering: investigation of storage pathways
abstract: A cybernetic model is proposed to examine generic features of storage pathways. This model is capable of describing synthesis of carbon and non-carbon storage polymers. The effect of environmental conditions is evaluated using storage polymer level as a fraction of total biomass as a gauge of pathway performance. The base wild-type pathway is then analyzed to determine the effect of genetic alterations upon system performance. Proposed modifications are tested using the cybernetic model as a diagnostic tool to ascertain the ramifications of potential genetic alterations. A methodology is developed within the cybernetic framework to describe alterations of enzyme activity and over-expression of pathway enzymes. Copyright 1998 John Wiley & Sons, Inc.
authors: School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
authors: J
authors: Varner
authors: D
authors: Ramkrishna