title: Human follicular dendritic cells remain uninfected and capture human immunodeficiency virus type 1 through CD54-CD11a interaction.
abstract: It has been reported that human immunodeficiency virus type 1 (HIV-1) bound to follicular dendritic cells (FDCs) remains highly infectious to CD4(+) T cells even when it forms immune complexes with neutralizing antibody (HIV-1/IC). To elucidate the role of FDCs in HIV-1 transmission to CD4(+) T cells in lymph nodes, we have isolated and purified FDCs from human tonsils and examined whether the HIV-1/IC trapped on their surface is infectious to CD4(+) T cells. To our surprise, not the HIV-1/IC but the antibody-free HIV-1 on FDCs could be transmitted to CD4(+) T cells. Furthermore, in contrast to previous studies showing that FDCs are productively infected with HIV-1, the present study clearly demonstrated that FDCs were not the target cells for HIV-1 infection. FDCs could capture the viral particles on their surface; however, the binding of HIV-1 to FDCs was strongly inhibited by the presence of anti-CD54 (ICAM-1) monoclonal antibody (MAb) and anti-CD11a (LFA-1) MAb, suggesting that the adhesion molecules play an important role in the interaction between HIV-1 and FDCs.
authors: Rational Drug Design Laboratories, Fukushima 960-1242, School of Medicine, Fukushima Medical University, Fukushima 960-1247, Japan.
authors: M
authors: M
authors: Fujiwara
authors: R
authors: R
authors: Tsunoda
authors: S
authors: S
authors: Shigeta
authors: T
authors: T
authors: Yokota
authors: M
authors: M
authors: Baba
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000821:virology; D002450:Cell Communication; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000378:metabolism* / Q000821:virology; D015497:HIV-1 / Q000378:metabolism*; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008196:Lymph / Q000166:cytology; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism*
chemical_list: D016169:Lymphocyte Function-Associated Antigen-1; D018799:Intercellular Adhesion Molecule-1