title: Propionic acid stimulates superoxide generation in human neutrophils.
abstract: Short-chain carboxylic acids are the metabolic by-products of pathogenic anaerobic bacteria and are found at sites of infection in millimolar quantities. We previously reported that propionic acid, one of the short-chain carboxylic acids, induces an increase in intracellular Ca2+ ([Ca2+]i) in human neutrophils. Here we investigate the effect of propionic acid on superoxide generation in human neutrophils. Propionic acid (10 mm) induced inositol 1,4, 5-trisphosphate (IP3) formation and a rapidly transient increase in [Ca2+]i, but not superoxide generation, whereas 1 microm formylmethionyl-leucyl-phenylalanine (fMLP), a widely used neutrophil-stimulating bacterial peptide, stimulated not only IP3 formation and Ca2+ mobilization but also superoxide generation. The IP3 level induced by propionic acid was slightly lower than that induced by fMLP. The transient increase in [Ca2+]i induced by propionic acid immediately returned to the basal level, whereas a sustained increase in [Ca2+]i, which was higher than the basal level, following a transient increase in [Ca2+]i was induced by fMLP. The peak level induced by propionic acid was lower than that with fMLP. In the absence of extracellular Ca2+, thapsigargin, a potent inhibitor of endoplasmic reticulum Ca2+-ATPase, induced an increase in [Ca2+]i even after propionic acid stimulation, but not after fMLP. The Ca2+ ionophore A23187 and thapsigargin induced superoxide generation by themselves. Propionic acid enhanced the superoxide generating effect of A23187 and thapsigargin. These results suggest that Ca2+ mobilization induced by propionic acid is much weaker than that with fMLP, and propionic acid is able to generate superoxide in the presence of a Ca2+ ionophore and a Ca2+ influx activator.
authors: Department of 1Pharmacology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, 271-8587, Japan.
authors: S
authors: S
authors: Nakao
authors: Y
authors: Y
authors: Moriya
authors: S
authors: S
authors: Furuyama
authors: R
authors: R
authors: Niederman
authors: H
authors: H
authors: Sugiya
mesh_terms: D000328:Adult; D002118:Calcium / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000097:blood; D007700:Kinetics; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology; D011422:Propionates / Q000494:pharmacology*; D013481:Superoxides / Q000097:blood*; D019284:Thapsigargin / Q000494:pharmacology
chemical_list: D011422:Propionates; D013481:Superoxides; D009240:N-Formylmethionine Leucyl-Phenylalanine; D019284:Thapsigargin; D015544:Inositol 1,4,5-Trisphosphate; C029658:propionic acid; D002118:Calcium