The age-dependent cytokine production by murine CD8+ T cells as determined by four-color flow cytometry analysis.
Flow cytometry has been used to simultaneously examine intracellular cytokine production and expression of CD44 and CD45RB by murine CD8+ T cells derived from young (2-3 months) or aged (18-22 months) mice. Cytokine production by aged CD8+ T cells differs from that by CD8+ T cells derived from young animals in that a significantly higher percentage of the aged can be triggered to produce interleukin (IL)-4, interferon (IFN)-gamma, and tumor necrosis factor alpha (TNF alpha). Conversely, a greater fraction of young CD8+ T cells produce IL-2. Aged mice not only have a higher percentage of CD8+/CD44hi T cells, but also a larger fraction of these cells are IFN-gamma+ and IL-4+, while a lower fraction are IL-2+ than is observed in young CD8+/CD44hi T cells. In terms of relative contribution to total cytokine synthesis, a greater fraction of CD8+ T cells produce IFN-gamma and IL-4 than in CD4+ T cells, whereas CD4+ T cells are the major producers of IL-2.
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Department of Immunology, Sidney Kimmel Cancer Center, San Diego, California, USA.
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D000375:Aging / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000378:metabolism*; D003116:Color; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D018960:Hyaluronan Receptors / Q000235:genetics; D007202:Indicators and Reagents; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D017493:Leukocyte Common Antigens / Q000235:genetics; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010798:Phycocyanin; D010799:Phycoerythrin; D019809:Streptavidin; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D016207:Cytokines; D005456:Fluorescent Dyes; D018960:Hyaluronan Receptors; D007202:Indicators and Reagents; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha; C002497:allophycocyanin; D010798:Phycocyanin; D010799:Phycoerythrin; D015847:Interleukin-4; D007371:Interferon-gamma; D019809:Streptavidin; D017493:Leukocyte Common Antigens; D016650:Fluorescein-5-isothiocyanate