title: Determination of ferrocene-A in the blood serum of rabbits using reversed-phase microcolumn HPLC.
abstract: Ferrocene-A (ferrocenyl-1-phenyl-1-dioxy-1,4-butin-2) has shown significant antitumour and antibacterial activity. To facilitate studies in this field a simple and rapid liquid chromatographic procedure was developed, allowing the determination of ferrocene-A in the blood serum. The procedure was based on a reversed-phase high-performance liquid chromatography (HPLC) method, with UV detection at 210 nm. The mobile phase ethanol-0.01 M KH2PO4 60:40 (v/v) provides the complete separation of ferrocene-A, internal standard and endogenous compounds of serum. The recovery of ferrocene-A from serum using a hexaneisoamyl alcohol mixture was 90%. The proposed method is convenient for pharmacological and pharmacokinetic applications.
authors: Department of Chemistry, Tbilisi State University, Republic of Georgia.
authors: M Sh
authors: MSh
authors: Pochkhidze
authors: L P
authors: LP
authors: Asatiani
authors: M D
authors: MD
authors: Rukhadze
authors: Z J
authors: ZJ
authors: Chitiashvili
authors: M A
authors: MA
authors: Tsartsidze
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005296:Ferrous Compounds / Q000097:blood*; D000075163:Metallocenes; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D005296:Ferrous Compounds; D000075163:Metallocenes; C004998:ferrocene