Langmuir-Blodgett films of antibodies as mediators of endothelial cell adhesion on polyurethanes.
The effect of endothelial cell adhesion on polyurethanes coated with Langmuir-Blodgett antibody films has been examined. The films were cross-linked with glutaraldehyde with the aim of providing a densely packed and covalently linked two-dimensional antibody network on the polyurethane surfaces. Our results demonstrate that although neither of the two polyurethanes examined were entirely suited to cellular adhesion, Langmuir-Blodgett antibody films, cross-linked with small concentrations of glutaraldehyde, are more suitable for endothelial cell adhesion than surfaces free of antibody.
Ahluwalia
A
A
Centro E. Piaggio, University of Pisa, Italy. arti@piaggio.ccii.unipi.it
Basta
G
G
Ricci
D
D
Francesconi
R
R
Domenici
C
C
Grattarola
M
M
Palchetti
L
L
Preininger
C
C
De Rossi
D
D
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000378:metabolism; D015703:Antigens, CD; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism; D015374:Biosensing Techniques; D002448:Cell Adhesion; D003432:Cross-Linking Reagents; D000071063:Endoglin; D004730:Endothelium, Vascular / Q000166:cytology*; D005976:Glutaral / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D011140:Polyurethanes / Q000737:chemistry*; D011956:Receptors, Cell Surface; D013499:Surface Properties; D019010:Vascular Cell Adhesion Molecule-1 / Q000276:immunology
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; C064206:ENG protein, human; D000071063:Endoglin; D011140:Polyurethanes; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D005976:Glutaral