Expression of interleukin-2 (IL-2) receptor alpha and CD45RO antigen on T-lymphocytes cultured with measles virus antigens, compared with humoral immunity in measles vaccinees.
In response to two types of measles virus (MV) antigens, a vaccine strain CAM and a wild strain isolated in 1994, the expression of IL-2 receptor alpha (CD25)(+)CD45RO(+)CD4(+) T-lymphocytes (T-cell activation) was analyzed by flow cytometry. In 75 healthy subjects with measles hemagglutination inhibition tests > or =1:16, the percentage of T-cell activation was significantly increased compared with that in seronegative individuals (p) < 0.05). Moreover, the T-cell expression was not significantly different among the vaccinated (n = 38), the naturally infected (n = 28) and the subclinically infected (exposed with wild type without history of measles infection and HI titers > or =1:16) (n = 10) groups. T-cell activation stimulated with MV antigens and HI antibody titers persisted for almost 30 years in the vaccinated group. These results suggest that cell-mediated immunity persists for long periods after vaccination and does not be influenced by antigenic drift.
Toyoda
M
M
Department of Pediatrics, National Mie Hospital, Tsu, Japan.
Ihara
T
T
Nakano
T
T
Ito
M
M
Kamiya
H
H
D000293:Adolescent; D000328:Adult; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology*; D015704:CD4 Antigens / Q000096:biosynthesis; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D017493:Leukocyte Common Antigens / Q000096:biosynthesis*; D008297:Male; D008458:Measles Vaccine / Q000276:immunology*; D008459:Measles virus / Q000276:immunology*; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine / Q000276:immunology; D015375:Receptors, Interleukin-2 / Q000096:biosynthesis*; D012411:Rubella Vaccine / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology; D017778:Vaccines, Combined / Q000276:immunology
D000956:Antigens, Viral; D015704:CD4 Antigens; D008458:Measles Vaccine; D022542:Measles-Mumps-Rubella Vaccine; D009108:Mumps Vaccine; D015375:Receptors, Interleukin-2; D012411:Rubella Vaccine; D017778:Vaccines, Combined; D017493:Leukocyte Common Antigens