Organization of the genes encoding the human proteasome activators PA28alpha and beta.
Two proteasome activators PA28alpha and beta, which have been implicated in antigen processing for loading class I MHC molecules, are synthesized in response to Ifn-gamma. The human genes encoding these activators (PSME1 and PSME2, respectively) were analyzed by sequencing. Each gene comprised 11 exons, consistent with gene duplication during vertebrate evolution. The intron/exon organization of both genes was highly conserved, the major difference being the absence of the exon encoding the lysine and glutamic acid-rich 'KEKE' motif in PA28beta. Two other genes of relevance to the immune system were located close to those for PA28 at 14q11.2 including ISGF3G, a protein involved in transcription after IFNalpha signalling. These sequences were also characterized.
McCusker
D
D
Division of Immunology, Department of Pathology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, UK.
Wilson
M
M
Trowsdale
J
J
D000595:Amino Acid Sequence; D017951:Antigen Presentation; D001483:Base Sequence; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D015698:Genomic Library; D015395:Histocompatibility Antigens Class I / Q000276:immunology; D006801:Humans; D008969:Molecular Sequence Data; D009124:Muscle Proteins; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics
D015395:Histocompatibility Antigens Class I; D009124:Muscle Proteins; C085047:PSME1 protein, human; D011506:Proteins; D014157:Transcription Factors; C486207:PSME2 protein, human; D046988:Proteasome Endopeptidase Complex