title: Membrane targeting and stabilization of sarcospan is mediated by the sarcoglycan subcomplex.
abstract: The dystrophin-glycoprotein complex (DGC) is a multisubunit complex that spans the muscle plasma membrane and forms a link between the F-actin cytoskeleton and the extracellular matrix. The proteins of the DGC are structurally organized into distinct subcomplexes, and genetic mutations in many individual components are manifested as muscular dystrophy. We recently identified a unique tetraspan-like dystrophin-associated protein, which we have named sarcospan (SPN) for its multiple sarcolemma spanning domains (Crosbie, R.H., J. Heighway, D.P. Venzke, J.C. Lee, and K.P. Campbell. 1997. J. Biol. Chem. 272:31221-31224). To probe molecular associations of SPN within the DGC, we investigated SPN expression in normal muscle as a baseline for comparison to SPN's expression in animal models of muscular dystrophy. We show that, in addition to its sarcolemma localization, SPN is enriched at the myotendinous junction (MTJ) and neuromuscular junction (NMJ), where it is a component of both the dystrophin- and utrophin-glycoprotein complexes. We demonstrate that SPN is preferentially associated with the sarcoglycan (SG) subcomplex, and this interaction is critical for stable localization of SPN to the sarcolemma, NMJ, and MTJ. Our experiments indicate that assembly of the SG subcomplex is a prerequisite for targeting SPN to the sarcolemma. In addition, the SG- SPN subcomplex functions to stabilize alpha-dystroglycan to the muscle plasma membrane. Taken together, our data provide important information about assembly and function of the SG-SPN subcomplex.
authors: Howard Hughes Medical Institute, Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
authors: R H
authors: RH
authors: Crosbie
authors: C S
authors: CS
authors: Lebakken
authors: K H
authors: KH
authors: Holt
authors: D P
authors: DP
authors: Venzke
authors: V
authors: V
authors: Straub
authors: J C
authors: JC
authors: Lee
authors: R M
authors: RM
authors: Grady
authors: J S
authors: JS
authors: Chamberlain
authors: J R
authors: JR
authors: Sanes
authors: K P
authors: KP
authors: Campbell
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D016466:CHO Cells; D002312:Cardiomyopathy, Hypertrophic / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002352:Carrier Proteins / Q000378:metabolism*; D006224:Cricetinae; D003598:Cytoskeletal Proteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D049030:Dystroglycans; D016189:Dystrophin / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008297:Male; D008562:Membrane Glycoproteins / Q000172:deficiency / Q000235:genetics / Q000502:physiology*; D008565:Membrane Proteins / Q000172:deficiency / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D008958:Models, Molecular; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D009137:Muscular Dystrophy, Animal / Q000235:genetics / Q000378:metabolism*; D009363:Neoplasm Proteins; D009469:Neuromuscular Junction / Q000378:metabolism; D011817:Rabbits; D049031:Sarcoglycans; D012508:Sarcolemma / Q000378:metabolism / Q000648:ultrastructure; D016415:Sequence Alignment; D013045:Species Specificity; D013710:Tendons / Q000378:metabolism; D014162:Transfection; D049411:Utrophin
chemical_list: D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; C483081:DAG1 protein, human; D016189:Dystrophin; D046911:Macromolecular Substances; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins; C109646:SSPN protein, human; D049031:Sarcoglycans; C491632:Sspn protein, mouse; C483600:Utrn protein, mouse; D049411:Utrophin; D049030:Dystroglycans