[The sorption kinetics in an immunosorbent of circulating immune reactants in the blood of patients with systemic lupus erythematosus].
In a double-blind trial blood content was studied of antibodies to nDNA and dDNA (AB-nDNA and AB-dDNA) using methods of solid-phase and liquid-phase immunoassay, of circulating immune complexes (CIC), beta 2-microglobulin (beta 2-MG), Ig G, M, and A during the time-related run of two sessions of immunosorption (IS) or nonselective hemosorption (NHS) in 22 patients with systemic lupus erythematosus. In eleven patients there was employed for IS DNA-containing active carbon, in other eleven patients carbon was used for NHS. Throughout the whole IS session high- and low-avid AB-nDNA and CIC were getting fixed with the sorbent followed by AB-dDNA, with the concentration gradient for these substances in the column being greater at minute 30 than it was at minute 15. The control carbon fixed AB-nDNA and AB-dDNA as measured by ELISA but at the start of the session it did so also with low-avid AB-at minute 30. At minute 15 of perfusion the blood level of low-avid AB-nDNA and AB-dDNA following the column procedure tended to significantly increase both in NHS and, to a lesser extent, in IS. Neither of the sorbents adsorbed IG. Decline in beta 2-MG concentration in columns with engrafted and matrix carbon at the start of the perfusion and its rise during the second half of the session might be caused by blood cells structural and functional condition as a result of their interaction with the sorbent in question.
Iaremenko
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D000915:Antibody Affinity; D004247:DNA / Q000276:immunology; D004311:Double-Blind Method; D006464:Hemoperfusion / Q000379:methods; D006801:Humans; D007136:Immunoglobulins / Q000097:blood; D007164:Immunosorbents / Q000097:blood*; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000628:therapy; D008181:Lupus Nephritis / Q000097:blood / Q000628:therapy; D018709:Statistics, Nonparametric; D013997:Time Factors
D007136:Immunoglobulins; D007164:Immunosorbents; D004247:DNA