Protective effect of flavonoids on endothelial cells against linoleic acid hydroperoxide-induced toxicity.
The protective effect of flavonoids against linoleic acid hydroperoxide (LOOH)-induced cytotoxicity was examined by using cultured endothelial cells. When the cells were incubated with both LOOH and flavonoids, most flavonols protected the cells from injury by LOOH. Flavones bearing an ortho-dihydroxy structure also showed a protective effect against the cytotoxicity of LOOH. However, flavanones had no effect. The structure-activity relationship revealed the presence of either the ortho-di-hydroxy structure in the B ring of the flavonoids or 3-hydroxyl and 4-oxo groups in the C ring to be important for the protective activities. The interaction between flavonoids and a-tocopherol was also examined in this system. Flavonoids that were protective against LOOH-induced cytotoxicity had at least an additive effect on the action of alpha-tocopherol against LOOH-induced damage.
Kaneko
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T
Tokyo Metropolitan Institute of Gerontology, Japan. kaneko@center.tmig.or.jp
Baba
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N
D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D047309:Flavones; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D044948:Flavonols; D006801:Humans; D044949:Kaempferols; D008041:Linoleic Acids / Q000633:toxicity*; D008054:Lipid Peroxides / Q000633:toxicity*; D047311:Luteolin; D009153:Mutagens / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014471:Umbilical Veins; D014810:Vitamin E / Q000494:pharmacology*
D000975:Antioxidants; D002867:Chromones; D004791:Enzyme Inhibitors; D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D044949:Kaempferols; D008041:Linoleic Acids; D008054:Lipid Peroxides; D009153:Mutagens; D014810:Vitamin E; C037032:galangin; C008285:linoleic acid hydroperoxide; C006552:kaempferol; C008548:morin; D011794:Quercetin; D047311:Luteolin; C017875:fisetin; C079163:quercetagetin