title: Comparative study of techniques used to recover viruses from residual urban sludge.
abstract: Eight virus extraction techniques were compared on three types of residual urban sludge for simultaneous detection of infectious enteroviruses, somatic coliphages, F-specific RNA bacteriophages and Bacteroides fragilis bacteriophages. The highest virus counts were found in extracts obtained using three extraction techniques described by respectively using a 10% beef extract solution at pH 9 and sonication, using a 0.3 M NaCl/7% beef extract solution at pH 7.5 and freon, and finally using a 0.1 M borate buffer/3% beef extract solution at pH 9 and sonication.
authors: U.M.R. University CNRS 7564, Laboratory of Virology, Faculty of Pharmacy, Nancy, France. lschwart@pharma.u-nancy.fr
authors: B
authors: B
authors: Mignotte
authors: A
authors: A
authors: Maul
authors: L
authors: L
authors: Schwartzbrod
mesh_terms: D001435:Bacteriophages / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000821:virology; D003090:Coliphages / Q000302:isolation & purification; D004770:Enterovirus / Q000302:isolation & purification*; D006801:Humans; D007220:Industrial Waste; D012317:RNA Phages / Q000302:isolation & purification; D012722:Sewage / Q000821:virology*; D010948:Viral Plaque Assay; D014871:Water Microbiology
chemical_list: D007220:Industrial Waste; D012722:Sewage