title: Effects of an Onchocerca-derived cysteine protease inhibitor on microfilariae in their simuliid vector.
abstract: A recombinant cysteine protease inhibitor, onchocystatin of the parasitic nematode Onchocerca volvulus, was tested for its role in microfilarial development in the simuliid vector. Onchocystatin was found to be present in female adults and skin microfilariae of the bovine parasite O. ochengi, the closest relative of O. volvulus. In addition the inhibitor could be detected as an excretory-secretory (E-S) product of the microfilariae. Co-injection of onchocystatin and the O. ochengi microfilariae into the surrogate vector Simulium ornatum s.l. significantly enhanced the recovery rates of the parasite within 24 h into the infection (P > 0.001). The findings suggest a possible role of onchocystatin in the evasion by the parasite of the immune response of its vector.
authors: Department of Biological Sciences, University of Salford, UK. S.Klager@biosci.salford.ac.uk
authors: S L
authors: SL
authors: Kläger
authors: H E
authors: HE
authors: Hagen
authors: J E
authors: JE
authors: Bradley
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000469:parasitology; D015853:Cysteine Proteinase Inhibitors / Q000378:metabolism*; D015801:Helminth Proteins / Q000378:metabolism*; D006790:Host-Parasite Interactions; D015151:Immunoblotting; D007303:Insect Vectors / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009854:Onchocerca / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D009855:Onchocerciasis / Q000469:parasitology / Q000662:veterinary; D012843:Simuliidae / Q000276:immunology / Q000469:parasitology*
chemical_list: D015853:Cysteine Proteinase Inhibitors; D015801:Helminth Proteins; C076278:Onchocystatin protein, Onchocerca volvulus