Fusicoccin, 14-3-3 proteins, and defense responses in tomato plants.
Fusicoccin (FC) is a fungal toxin that activates the plant plasma membrane H+-ATPase by binding with 14-3-3 proteins, causing membrane hyperpolarization. Here we report on the effect of FC on a gene-for-gene pathogen-resistance response and show that FC application induces the expression of several genes involved in plant responses to pathogens. Ten members of the FC-binding 14-3-3 protein gene family were isolated from tomato (Lycopersicon esculentum) to characterize their role in defense responses. Sequence analysis is suggestive of common biochemical functions for these tomato 14-3-3 proteins, but their genes showed different expression patterns in leaves after challenges. Different specific subsets of 14-3-3 genes were induced after treatment with FC and during a gene-for-gene resistance response. Possible roles for the H+-ATPase and 14-3-3 proteins in responses to pathogens are discussed.
Roberts
M R
MR
The Plant Laboratory, Department of Biology, University of York, P.O. Box 373, York YO10 5YW, United Kingdom. mrr5@york.ac.uk
Bowles
D J
DJ
D048948:14-3-3 Proteins; D000595:Amino Acid Sequence; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D017343:Genes, Plant; D006027:Glycosides / Q000494:pharmacology*; D006801:Humans; D018551:Solanum lycopersicum / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014446:Tyrosine 3-Monooxygenase
D048948:14-3-3 Proteins; D017931:DNA Primers; D006027:Glycosides; D010940:Plant Proteins; D011506:Proteins; C007808:fusicoccin; D014446:Tyrosine 3-Monooxygenase