Type 1 CD4(+) T-cell help is required for induction of antipeptide multispecific cytotoxic T lymphocytes by a lipopeptidic vaccine in rhesus macaques.
We have optimized the induction of antiviral cytotoxic T lymphocytes (CTL) in rhesus macaques by a lipopeptide vaccine containing seven peptides from simian immunodeficiency virus (SIV) Nef and Gag proteins and a strong T-helper peptide from tetanus toxoid (TT) that is promiscuous in humans (peptide TT 830-846). Two of the eight immunized macaques showed T-helper (Th) cell proliferation and a specific synthesis of gamma interferon in response to TT 830-846 peptide. They also showed multispecific cytotoxic activity against three to five of the immunizing SIV peptides. These results show the importance of a strong specific type 1 Th response for inducing a multispecific CTL response in vivo, which is essential for the development of an anti-human immunodeficiency virus vaccine.
Mortara
L
L
Institut Cochin de Génétique Moléculaire (ICGM), Laboratoire d'Immunologie des Pathologies Infectieuses et Tumorales, INSERM U445-Université René Descartes, Hôpital Cochin, 75014 Paris, France.mortara@cochin.inser.fr
Gras-Masse
H
H
Rommens
C
C
Venet
A
A
Guillet
J G
JG
Bourgault-Villada
I
I
D000595:Amino Acid Sequence; D000818:Animals; D015683:Gene Products, gag / Q000276:immunology*; D015693:Gene Products, nef / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008074:Lipoproteins / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
D015683:Gene Products, gag; D015693:Gene Products, nef; D000949:Histocompatibility Antigens Class II; D008074:Lipoproteins; D010455:Peptides; D018449:SAIDS Vaccines; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; D007371:Interferon-gamma