Active and passive immunization with the Pseudomonas V antigen protects against type III intoxication and lung injury.
Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause fatal acute lung infections in critically ill individuals. Damage to the lung epithelium is associated with the expression of toxins that are directly injected into eukaryotic cells through a type Ill-mediated secretion and translocation mechanism. Here we show that the P. aeruginosa homolog of the Yersinia V antigen, PcrV, is involved in the translocation of type III toxins. Vaccination against PcrV ensured the survival of challenged mice and decreased lung inflammation and injury. Antibodies to PcrV inhibited the translocation of type III toxins.
Sawa
T
T
Department of Anesthesia and Perioperative Care, The University of California, San Francisco 94143-0542, USA.
Yahr
T L
TL
Ohara
M
M
Kurahashi
K
K
Gropper
M A
MA
Wiener-Kronish
J P
JP
Frank
D W
DW
D000818:Animals; D000907:Antibodies, Bacterial / Q000494:pharmacology; D000942:Antigens, Bacterial / Q000235:genetics / Q000506:poisoning / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism / Q000506:poisoning*; D001427:Bacterial Toxins / Q000235:genetics / Q000506:poisoning / Q000627:therapeutic use*; D001692:Biological Transport; D002470:Cell Survival; D005798:Genes, Bacterial; D007114:Immunization / Q000379:methods*; D007116:Immunization, Passive / Q000379:methods; D008171:Lung Diseases / Q000628:therapy*; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010587:Phagocytosis; D052899:Pore Forming Cytotoxic Proteins; D011552:Pseudomonas Infections / Q000628:therapy*; D014611:Vaccination / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D052899:Pore Forming Cytotoxic Proteins; C118546:antigen V, Pseudomonas