Burst production of superoxide anion in human endothelial cells by lysophosphatidylcholine.
This study examined whether lysophosphatidylcholine (lysoPC), an atherogenic lipid, may stimulate production of O2*-, in cultured human endothelial cells. Production of O2*- was detected by bis-N-methylacridinium nitrate (lucigenin)-elicited chemiluminescence. LysoPC was found to induce burst production of O2*-, peaked at 2-4 min after the stimulation, in intact endothelial cells. LysoPC also stimulated NADH-dependent production of O2*- in particulate fraction of the cells, and the action of lysoPC was inhibited by diphenyliodonium. The results suggested that lysoPC stimulated production of O2*- partly through membrane-associated NADH-dependent O2*- production systems.
Kugiyama
K
K
Division of Cardiology, Kumamoto University School of Medicine, Japan. kiyo@gpo.kumamoto-u.ac.jp
Sugiyama
S
S
Ogata
N
N
Oka
H
H
Doi
H
H
Ota
Y
Y
Yasue
H
H
D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / Q000494:pharmacology; D000166:Acridines; D001713:Biphenyl Compounds / Q000494:pharmacology; D002458:Cell Fractionation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D008244:Lysophosphatidylcholines / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D009861:Onium Compounds / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism*
D000166:Acridines; D001713:Biphenyl Compounds; D008244:Lysophosphatidylcholines; D009861:Onium Compounds; D012402:Rotenone; D009243:NAD; C031291:diphenyliodonium; D013481:Superoxides; C033472:10,10'-dimethyl-9,9'-biacridinium; D014013:1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; D009249:NADP; D013482:Superoxide Dismutase