Site-specific derivatization of RNA with photocrosslinkable groups.
Derivatization of RNA with heterobifunctional photocrosslinking reagents becomes an increasingly popular method for the analysis of structural properties of ribonucleoprotein complexes. This article describes a simple chemical modification-derivatization strategy used to introduce selected chemical groups at specific internal positions within the RNA ribose backbone. The strategy is based on the coupling of a haloacetyl adduct to a thiol residue in the phosphodiester bond. The use of a number of RNA probes derivatized with several different photoreactive groups can provide invaluable information on the structural distribution of components in complex ribonucleoprotein assemblies.
Konarska
M M
MM
Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10021, USA.
D001386:Azides / Q000494:pharmacology; D001577:Benzophenones / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008956:Models, Chemical; D019920:Photoaffinity Labels / Q000494:pharmacology; D012313:RNA / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D014466:Ultraviolet Rays
D001386:Azides; D001577:Benzophenones; D003432:Cross-Linking Reagents; D019920:Photoaffinity Labels; D012313:RNA; D012266:Ribose; C047723:benzophenone; D012259:Ribonuclease, Pancreatic; C011171:4-azidophenacyl bromide