[Polymorphisms of five short tandem repeat systems in Chinese Han population in Chengdu].
OBJECTIVE
To get preliminary genotype and allele frequency distributions of D6S366, D6S477, D12S375, D12S391 and PLA2A loci in Chinese Han population in Chengdu area and to validate more short Tandem repeat (STR) systems for forensic application.


METHODS
The polymerase chain reaction (PCR) was used. Five STR systems (D6S366, D6S477, D12S375, D12S391 and PLA2A) were amplified on DNA samples, which were extracted from 108 unrelated individuals EDTA-blood by Phenol/Chloroform method. The PCR products were analyzed by PAG vertical electrophoresis.


RESULTS
Seven alleles were found at D6S366 locus, nine alleles at D6S477 locus, five alleles at D12S375 locus, nine alleles at D12S391 locus and seven alleles at PLA2A locus. No deviations from Hardy-Weinberg equilibrium were observed. The heterozygosities observed were 0.69, 0.78, 0.81, 0.68 and 0.79 for D6S366, D6S477, D12S375, D12S391 and PLA2A respectively. The chances of exclusion were 0.3621, 0.6004, 0.4581, 0.7014 and 0.5589 and the discriminating powers were 0.79, 0.93, 0.86, 0.96 and 0.91.


CONCLUSIONS
All of the five loci in this study were useful markers for individual identification and paternity test and for genetics purposes.
Chen
G
G
Institute of Forensic Medicine, West China University of Medical Sciences. Chengdu, 610041, P. R. China. fyxchen@mail.sc.cninfo.net
Xin
J
J
Li
Y
Y
Wu
J
J
Hou
Y
Y
Li
J
J
Deng
S
S
D000483:Alleles; D002681:China / Q000208:ethnology; D002874:Chromosome Mapping; D006801:Humans; D011110:Polymorphism, Genetic; D020080:Tandem Repeat Sequences