title: T cell immunity induced by allogeneic microglia in relation to neuronal retina transplantation.
abstract: Microglia share a lineage relationship with bone marrow-derived monocytes/macrophages and dendritic cells, and their inclusion in retinal and brain transplants may function as "passenger leukocytes. " In other solid allografts, passenger leukocytes are the primary sources of immunogenicity, triggering alloimmune rejection. We have conducted a series of in vitro and in vivo studies examining the capacity of microglia cultured from forebrain to activate alloreactive T cells and to induce and elicit alloimmunity. Cultured microglia expressed class II MHC molecules and costimulatory molecules (B7-1, B7-2, and CD40), and they secreted IL-12. Cultured microglia injected s.c. into naive recipients induced allospecific delayed hypersensitivity and elicited delayed hypersensitivity directed at alloantigens. Cultured microglia differed from conventional APCs by secreting significant amounts of mature TGF-beta2, but smaller amounts of IL-12. Moreover, while both cultured microglia and conventional APC stimulated T cell proliferation in vitro, microglia directed the responding T cells toward the Th2 pathway in which IL-4, but not IL-2 and IFN-gamma, was secreted. The abilities of microglia to secrete TGF-beta2, to stimulate alloreactive Th2 cells, and to induce anterior chamber associated immune deviation when injected into the eye of naive allogeneic mice suggest that they are not typical passenger leukocytes. The unique functional properties of cultured microglia may account for the capacity of neonatal retinal tissue transplanted into the eye to alter the systemic alloimmune response in a manner that delays, but does not prevent, graft rejection.
authors: Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA, 02114, USA.
authors: N
authors: N
authors: Ma
authors: J W
authors: JW
authors: Streilein
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000276:immunology; D000938:Antigen-Presenting Cells / Q000378:metabolism; D000954:Antigens, Surface / Q000096:biosynthesis; D001202:Ascitic Fluid / Q000166:cytology / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006968:Hypersensitivity, Delayed / Q000209:etiology; D007279:Injections, Subcutaneous; D018664:Interleukin-12 / Q000096:biosynthesis; D007519:Isoantigens / Q000096:biosynthesis / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D017628:Microglia / Q000276:immunology* / Q000378:metabolism / Q000637:transplantation; D009474:Neurons / Q000276:immunology / Q000637:transplantation*; D012160:Retina / Q000276:immunology / Q000637:transplantation*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D007519:Isoantigens; D016212:Transforming Growth Factor beta; D018664:Interleukin-12