title: Sequence polymorphism of the group 1 allergen of Bermuda grass pollen.
abstract: BACKGROUND
  Cyn d 1, the major allergen of Bermuda grass pollen, consists of a number of isoforms.
  
  
  OBJECTIVE
  To examine the extent of sequence variation of Cyn d 1 isoforms at the molecular level.
  
  
  METHODS
  A Bermuda grass pollen lambdaZAP II cDNA expression library was immunoscreened with anti-Cyn d 1 monoclonal antibodies. The reactive clones were isolated, subcloned into Escherichia coli, and sequenced. Some of them were expressed in the yeast Pichia pastoris to obtain recombinant Cyn d 1 proteins.
  
  
  RESULTS
  Ten cDNA clones were obtained, all these clones encode the full length of Cyn d 1 protein. Their deduced mature proteins can be grouped into: the long ones with 246 amino acids, and the short ones with 244 amino acids. The last two amino acids (AG) of the long Cyn d 1 are deleted in the short Cyn d 1. The remaining amino acid sequences share more than 98% identity; a total of nine amino acid variations were observed. Two recombinant Cyn d 1 proteins (rCyn d 3-2 and rCyn d 5-4) with three amino acid substitutions showed differential IgE-binding profiles.
  
  
  CONCLUSIONS
  The present study extended our understanding of the primary structure of isoforms of Cyn d 1.
authors: Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, Taiwan, Republic of China.
authors: Z N
authors: ZN
authors: Chang
authors: H J
authors: HJ
authors: Peng
authors: W C
authors: WC
authors: Lee
authors: T S
authors: TS
authors: Chen
authors: K Y
authors: KY
authors: Chua
authors: L C
authors: LC
authors: Tsai
authors: C W
authors: CW
authors: Chi
authors: S H
authors: SH
authors: Han
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D008969:Molecular Sequence Data; D008970:Molecular Weight; D006109:Poaceae / Q000276:immunology*; D011058:Pollen / Q000276:immunology*; D011110:Polymorphism, Genetic; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D000485:Allergens; D011994:Recombinant Proteins