Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells.
The induction of sister chromatid exchanges (SCEs) was evaluated in the cultured mouse m5S cells after exposure to extremely low frequency magnetic field (ELFMF; 5, 50 and 400 mT). Exposure to 5 mT and 50 mT ELFMF led to a very small increase in the frequency of SCEs, but no significant difference was observed between exposed and unexposed control cells. The cells exposed to 400 mT ELFMF exhibited a significant elevation of the SCE frequencies. There was no significant difference between data from treatments with mitomycin-C (MMC) alone and from combined treatments of MMC plus ELFMF (400 mT) at any MMC concentrations from 4 to 40 nM. These results suggest that exposure to highest-density ELFMF of 400 mT may induce DNA damage, resulting in an elevation of the SCE frequencies. We suppose that there may be a threshold for the elevation of the SCE frequencies, that is at least over the magnetic density of 50 mT.
Yaguchi
H
H
Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.
Yoshida
M
M
Ejima
Y
Y
Miyakoshi
J
J
D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D008280:Magnetics / Q000009:adverse effects*; D051379:Mice; D016685:Mitomycin / Q000633:toxicity*; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods*; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000528:radiation effects*
D009153:Mutagens; D016685:Mitomycin