title: Adhesion of cultured bovine aortic endothelial cells to laminin-1 mediated by dystroglycan.
abstract: Expression of dystroglycan (DG) by cultured bovine aortic endothelial (BAE) cells was confirmed by cDNA cloning from a BAE cDNA library, Northern blotting of mRNA, Western blotting of membrane proteins, and double immunostaining with antibodies against betaDG and platelet endothelial cell adhesion molecule-1. Immunocytochemical analysis revealed localization of DG in multiple plaques on the basal side of resting cells. This patchy distribution was obscured in migrating cells, in which the most prominent staining was observed in the trailing edge anchoring the cells to the substratum. Biotin-labeled laminin-1 overlay assay of dissociated BAE membrane proteins indicated the interaction of laminin-1 with alphaDG. The laminin alpha5 globular domain fragment expressed in bacteria and labeled with biotin could also bind alphaDG on the membrane blot, and the unlabeled fragment disrupted the binding of biotin-laminin-1 to alphaDG. The interaction of biotin-laminin-1 with alphaDG was inhibited by soluble alphaDG contained in the conditioned medium from DG cDNA-transfected BAE cells and by a series of glycosaminoglycans (heparin, dextran sulfate, and fucoidan). Soluble alphaDG in the conditioned medium inhibited the adhesion of BAE cells to laminin-1-coated dishes, whereas it had no effect on their adhesion to fibronectin. All three glycosaminoglycans that disrupted the biotin-laminin-1 binding to alphaDG inhibited BAE cell adhesion to laminin-1, whereas they failed to inhibit the adhesion to fibronectin. These results indicate a role of DG as a non-integrin laminin receptor involved in vascular endothelial cell adhesion to the extracellular matrix.
authors: Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan.
authors: H
authors: H
authors: Shimizu
authors: H
authors: H
authors: Hosokawa
authors: H
authors: H
authors: Ninomiya
authors: J H
authors: JH
authors: Miner
authors: T
authors: T
authors: Masaki
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism; D001483:Base Sequence; D001710:Biotin / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D017931:DNA Primers; D049030:Dystroglycans; D004730:Endothelium, Vascular / Q000166:cytology* / Q000378:metabolism; D007797:Laminin / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000378:metabolism*; D011485:Protein Binding; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D003598:Cytoskeletal Proteins; D017931:DNA Primers; D007797:Laminin; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins; D049030:Dystroglycans; D001710:Biotin