title: Transcript analysis of 250 novel yeast genes from chromosome XIV.
abstract: The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for 250 novel ORFs on chromosome XIV. All transcripts were quantified by Northern analysis under three quasi-steady-state conditions (exponential growth on rich fermentative, rich non-fermentative, and minimal fermentative media) and eight transient conditions (glucose derepression, glucose upshift, stationary phase, nitrogen starvation, osmo-stress, heat-shock, and two control conditions). Transcripts were detected for 82% of the 250 ORFs, and only one ORF did not yield a transcript of the expected length (YNL285w). Transcripts ranged from low (62%), moderate (16%) to high abundance (2%) relative to the ACT1 mRNA. The levels of 73% of the 206 chromosome XIV transcripts detected fluctuated in response to the transient states tested. However, only a small number responded strongly to the transients: eight ORFs were induced upon glucose upshift; five were repressed by glucose; six were induced in response to nitrogen starvation; three were induced in stationary phase; five were induced by osmo-stress; four were induced by heat-shock. These data provide useful clues about the general function of these ORFs and add to our understanding of gene regulation on a genome-wide basis.
authors: Dept. Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
authors: R J
authors: RJ
authors: Planta
authors: A J
authors: AJ
authors: Brown
authors: J L
authors: JL
authors: Cadahia
authors: M E
authors: ME
authors: Cerdan
authors: M
authors: M
authors: de Jonge
authors: M E
authors: ME
authors: Gent
authors: A
authors: A
authors: Hayes
authors: C P
authors: CP
authors: Kolen
authors: L J
authors: LJ
authors: Lombardia
authors: M
authors: M
authors: Sefton
authors: S G
authors: SG
authors: Oliver
authors: J
authors: J
authors: Thevelein
authors: H
authors: H
authors: Tournu
authors: Y J
authors: YJ
authors: van Delft
authors: D J
authors: DJ
authors: Verbart
authors: J
authors: J
authors: Winderickx
mesh_terms: D015152:Blotting, Northern; D015825:Chromosomes, Fungal / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D018869:Heat-Shock Response / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D012331:RNA, Fungal; D012333:RNA, Messenger