title: Reference values of urinary 3,5,6-trichloro-2-pyridinol in the Italian population--validation of analytical method and preliminary results (multicentric study).
abstract: The interlaboratory validation of analytical procedures for the assay of urinary 3,5,6-trichloro-2-pyridinol (TCP) in the general Italian population is reported. The determinations were performed by high-resolution gas chromatography (HRGS) with electron capture detection and HRGS with mass spectrometry (MS) in 2 laboratories. The urine samples were from 42 participants from 3 regions of Italy. The results were evaluated by interlaboratory quality control. Urinary TCP concentrations were above the detection limit (1.2 micrograms/L) in 88% of the population, with a mean detectable concentration [GM (GSD)] of 2.8 (1.9) micrograms/g creatinine (creat). (GM, geometric mean; GSD, geometric standard deviation.) The Mann-Whitney U test showed that wine consumption was a statistically significant variable (p < 0.05) for urinary concentrations of TCP. Analysis of variance of the logarithm of urinary TCP versus wine consumption and diet showed a statistically significant fit. The model used explained 30% of the total variance: wine consumption and diet accounted for 37 and 17% respectively of the explained variance.
authors: Institute of Occupational Medicine, Occupational Toxicology Unit, Siena, Italy.
authors: C
authors: C
authors: Aprea
authors: A
authors: A
authors: Betta
authors: G
authors: G
authors: Catenacci
authors: A
authors: A
authors: Lotti
authors: S
authors: S
authors: Magnaghi
authors: A
authors: A
authors: Barisano
authors: V
authors: V
authors: Passini
authors: I
authors: I
authors: Pavan
authors: G
authors: G
authors: Sciarra
authors: V
authors: V
authors: Vitalone
authors: C
authors: C
authors: Minoia
mesh_terms: D000328:Adult; D000818:Animals; D004390:Chlorpyrifos / Q000493:pharmacokinetics; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004032:Diet; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006207:Half-Life; D006801:Humans; D006868:Hydrolysis; D007306:Insecticides / Q000493:pharmacokinetics / Q000652:urine*; D007558:Italy; D008297:Male; D008875:Middle Aged; D010573:Pesticide Residues / Q000652:urine; D011728:Pyridones / Q000652:urine*; D011786:Quality Control; D012016:Reference Values; D014920:Wine
chemical_list: D007306:Insecticides; D010573:Pesticide Residues; D011728:Pyridones; C012587:3,5,6-trichloro-2-pyridinol; D004390:Chlorpyrifos