title: ORC localization in Drosophila follicle cells and the effects of mutations in dE2F and dDP.
abstract: We isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is localized throughout the follicle cell nuclei when they are undergoing polyploid genomic replication, and its levels appear constant in both S and G phases. In contrast, when genomic replication ceases and specific regions amplify, ORC2 is present solely at the amplifying loci. Mutations in the DNA-binding domains of dE2F or dDP reduce amplification, and in these mutants specific localization of ORC2 to amplification loci is lost. Interestingly, a dE2F mutant predicted to lack the carboxy-terminal transcriptional activation and RB-binding domain does not abolish ORC2 localization and shows premature chorion amplification. The effect of the mutations in the heterodimer subunits suggests that E2F controls not only the onset of S phase but also origin activity within S phase.
authors: Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02142 USA.
authors: I
authors: I
authors: Royzman
authors: R J
authors: RJ
authors: Austin
authors: G
authors: G
authors: Bosco
authors: S P
authors: SP
authors: Bell
authors: T L
authors: TL
authors: Orr-Weaver
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002352:Carrier Proteins; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000378:metabolism; D002823:Chorion / Q000378:metabolism; D019927:Cyclin E / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins; D050684:E2F Transcription Factors; D005260:Female; D005784:Gene Amplification; D005838:Genotype; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D009154:Mutation; D009866:Oogenesis / Q000235:genetics; D051738:Origin Recognition Complex; D010053:Ovary / Q000378:metabolism*; D010063:Ovum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D019927:Cyclin E; D004268:DNA-Binding Proteins; C088053:Dp transcription factor, Drosophila; D029721:Drosophila Proteins; D050684:E2F Transcription Factors; C497216:Orc2 protein, Drosophila; D051738:Origin Recognition Complex; D056465:Retinoblastoma-Binding Protein 1; D015534:Trans-Activators; D014157:Transcription Factors; D001616:beta-Galactosidase; D001973:Bromodeoxyuridine