Staphylococcus aureus expresses a cell surface protein that binds both IgG and beta2-glycoprotein I.
The existence of a second IgG-binding protein, protein Sbi, in Staphylococcus aureus has been reported previously. Later data indicated that protein Sbi also bound another serum component. This component has now been affinity-purified on immobilized protein Sbi and identified as beta2-glycoprotein I (beta2-GPI), also known as apolipoprotein H. The minimal beta2-GPI-binding domain was identified by shotgun phage display and the binding was shown to be mediated by a region of 57 amino acids, clearly separated from the IgG-binding domain. It is also shown that protein Sbi, and thus the beta2-GPI-binding activity, is expressed on the staphylococcal cell surface at levels varying between strains.
Zhang
Lihong
L
Jacobsson
Karin
K
Ström
Katrin
K
Lindberg
Martin
M
Frykberg
Lars
L
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins; D001435:Bacteriophages / Q000235:genetics; D001665:Binding Sites; D015153:Blotting, Western; D002352:Carrier Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002846:Chromatography, Affinity; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D019151:Peptide Library; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000737:chemistry / Q000378:metabolism*; D053482:beta 2-Glycoprotein I
D001426:Bacterial Proteins; D002352:Carrier Proteins; D006023:Glycoproteins; D007074:Immunoglobulin G; D008565:Membrane Proteins; D019151:Peptide Library; C112405:Sbi protein, Staphylococcus aureus; D053482:beta 2-Glycoprotein I