title: Identification of oral mitis group streptococci by arbitrarily primed polymerase chain reaction.
abstract: "Mitis group" streptococci are commensal but may play some role in dental caries, septicemia or endocarditis. Rapid genotypic identification would aid studies of dental plaque ecology, or diagnostic use. AP-PCR with 58 unpaired arbitrary primers was used to characterize 7 Streptococcus gordonii, 11 Streptococcus sanguis, 2 Streptococcus crista, 5 Streptococcus parasanguis, 18 Streptococcus oralis, and 36 Streptococcus mitis (22 biovar 1 and 14 biovar 2). S. parasanguis 16S rRNA variable region primer RR2 produced species-specific bands with all S. gordonii and S. sanguis. Human V beta 1 T-cell receptor primer 434 yielded concordant genotypic identification of all phenotypically defined S. crista and S. parasanguis, 83% of S. oralis, and 74% of S. mitis biovar 1. Amplicon patterns for S. mitis biovar 2 were heterogeneous. Findings suggest that primers RR2 and 434 in succession will allow rapid identification of genotypic groups corresponding closely to mitis group species established by phenotype.
authors: Department of Oral Science, School of Dentistry, University of Minnesota, Minneapolis 55455, USA.
authors: J D
authors: JD
authors: Rudney
authors: C J
authors: CJ
authors: Larson
mesh_terms: D015373:Bacterial Typing Techniques; D017931:DNA Primers; D018740:Genetic Heterogeneity; D005838:Genotype; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D010641:Phenotype; D019105:Random Amplified Polymorphic DNA Technique; D013045:Species Specificity; D013291:Streptococcus / Q000145:classification* / Q000235:genetics*; D019533:Streptococcus oralis / Q000145:classification / Q000235:genetics; D013298:Streptococcus sanguis / Q000145:classification / Q000235:genetics
chemical_list: D017931:DNA Primers