Derivatization of the interface cysteine of triosephosphate isomerase from Trypanosoma brucei and Trypanosoma cruzi as probe of the interrelationship between the catalytic sites and the dimer interface.
In the interface of homodimeric triosephosphate isomerase from Trypanosoma brucei (TbTIM) and Trypanosoma cruzi (TcTIM), one cysteine of each monomer forms part of the intersubunit contacts. The relatively slow derivatization of these cysteines by sulfhydryl reagents induces progressive structural alterations and abolition of catalysis [Garza-Ramos et al. (1998) Eur. J. Biochem. 253, 684-691]. Derivatization of the interface cysteine by 5, 5-dithiobis(2-nitrobenzoate) (DTNB) and methylmethane thiosulfonate (MMTS) was used to probe if events at the catalytic site are transmitted to the dimer interface. It was found that enzymes in the active catalytic state are significantly less sensitive to the thiol reagents than in the resting state. Maximal protection against derivatization of the interface cysteine by thiol reagents was obtained at near-saturating substrate concentrations. Continuous recording of derivatization by DTNB showed that catalysis hinders the reaction of sulfhydryl reagents with the interface cysteine. Therefore, in addition to intrinsic structural barriers, catalysis imposes additional impediments to the action of thiol reagents on the interface cysteine. In TcTIM, the substrate analogue phosphoglycolate protected strongly against DTNB action, and to a lesser extent against MMTS action; in TbTIM, phosphoglycolate protected against the effect of DTNB, but not against the action of MMTS. This indicates that barriers of different magnitude to the reaction of thiol reagents with the interface cysteine are induced by the events at the catalytic site. Studies with a Cys14Ser mutant of TbTIM confirmed that all the described effects of sulfhydryl reagents on the trypanosomal enzymes are a consequence of derivatization of the interface cysteine.
Pérez-Montfort
R
R
Departamento de Bioquímica, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México.
Garza-Ramos
G
G
Alcántara
G H
GH
Reyes-Vivas
H
H
Gao
X G
XG
Maldonado
E
E
de Gómez-Puyou
M T
MT
Gómez-Puyou
A
A
D000818:Animals; D002384:Catalysis; D003545:Cysteine / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D004228:Dithionitrobenzoic Acid / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005986:Glyceraldehyde 3-Phosphate / Q000494:pharmacology; D006016:Glycolates / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000031:analogs & derivatives / Q000037:antagonists & inhibitors / Q000494:pharmacology; D016297:Mutagenesis, Site-Directed; D012694:Serine / Q000235:genetics; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000037:antagonists & inhibitors / Q000494:pharmacology; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000737:chemistry* / Q000235:genetics; D014346:Trypanosoma brucei brucei / Q000201:enzymology* / Q000235:genetics; D014349:Trypanosoma cruzi / Q000201:enzymology*
D004791:Enzyme Inhibitors; D006016:Glycolates; D013439:Sulfhydryl Reagents; D005986:Glyceraldehyde 3-Phosphate; C014674:methyl methanethiosulfonate; D012694:Serine; D004228:Dithionitrobenzoic Acid; D008741:Methyl Methanesulfonate; D014305:Triose-Phosphate Isomerase; C013193:phosphoglycolate; D003545:Cysteine