Diversity of chlorophenol-degrading bacteria isolated from contaminated boreal groundwater.
Chlorophenol-degrading bacteria from a long-term polluted groundwater aquifer were characterized. All isolates degraded 2,4,6-trichlorophenol and 2,3,4,6-tetrachlorophenol at concentrations detected in the contaminated groundwater (< 10 mg 1(-1)). Pentachlorophenol was degraded by three isolates when present alone. In two gram-positive isolates, 2,3,4,6-tetrachlorophenol was required as an inducer for the degradation of pentachlorophenol. The gram-positive isolates were sensitive to pentachlorophenol, with an IC50 value of 5 mg/l. Isolates belonging to the Cytophaga/Flexibacter/Bacteroides phylum had IC50 values of 25 and 63 mg/l. Isolates belonging to alpha-, beta- and gamma-Proteobacteria generally tolerated the highest pentachlorophenol concentrations (> 100 mg/l). Polychlorophenol-degrading capacity was found in strains of Nocardioides, Pseudomonas, Ralstonia, Flavobacterium, and Caulobacter previously not known to degrade polychlorophenols. In addition, six polychlorophenol-degrading sphingomonads were found.
Männistö
M K
MK
Tampere University of Technology, Department of Environmental Technology, Finland.
Tiirola
M A
MA
Salkinoja-Salonen
M S
MS
Kulomaa
M S
MS
Puhakka
J A
JA
D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D001482:Base Composition; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D005618:Fresh Water; D014644:Genetic Variation; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010416:Pentachlorophenol / Q000378:metabolism; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D002733:Chlorophenols; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D010416:Pentachlorophenol