Simultaneous detection of Bacteroides forsythus and Prevotella intermedia by 16S rRNA gene-directed multiplex PCR.
In a 16S rRNA gene-directed multiplex PCR, Prevotella intermedia- and Bacteroides forsythus-specific reverse primers were combined with a single conserved forward primer. A 660-bp fragment and an 840-bp fragment that were specific for both species could be amplified simultaneously. A total of 152 clinical samples, subgingival plaque and swabs of three different oral mucosae, from 38 periodontitis patients were used for the evaluation.
Conrads
G
G
Clinic of Conservative and Preventive Dentistry and Periodontology, University Hospital (RWTH), D-52057 Aachen, Germany. conrads@alpha.imib.rwth-aachen.de
Flemmig
T F
TF
Seyfarth
I
I
Lampert
F
F
Lütticken
R
R
D000328:Adult; D000368:Aged; D001439:Bacteroides / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000382:microbiology; D016133:Polymerase Chain Reaction; D018713:Prevotella intermedia / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D012680:Sensitivity and Specificity
D012336:RNA, Ribosomal, 16S