Protection against tick-borne encephalitis virus isolated in Japan by active and passive immunization.
In order to establish a firm preventive measure for tick-borne encephalitis (TBE) in Japan, we evaluated the immune response of European vaccine against Japanese TBE virus strain (Oshima 5-10) for man and mouse. Furthermore, the efficacy of pre- and post-exposure protection by a polyclonal rabbit anti-TBE virus serum was examined in the mouse model. 80% of vaccinees seroconverted against Oshima 5-10 strain after the 2nd immunization of vaccine and the remaining 20% seroconverted after the 3rd immunization. Two persons with pre-existing anti-Japanese encephalitis virus (JEV) antibodies showed low immune responses against TBE virus. In mouse vaccination and challenge tests, efficient protection was observed in mice challenged with lethal doses of Oshima 5-10 strain as well as those observed in mice with the Western subtype and the Far Eastern subtype of TBE strains. Pre-exposure treatment with rabbit anti-TBE virus serum provided complete protection against lethal challenge with Oshima 5-10 strain. For post-exposure treatment with the antibody, significant protection was observed when mice were treated 24 h after virus challenge, whereas it was not observed 48 h after virus challenge. reserved.
Chiba
N
N
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Osada
M
M
Komoro
K
K
Mizutani
T
T
Kariwa
H
H
Takashima
I
I
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D006224:Cricetinae; D004669:Encephalitis Viruses, Tick-Borne / Q000276:immunology*; D004675:Encephalitis, Tick-Borne / Q000517:prevention & control*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D007116:Immunization, Passive; D007564:Japan; D008297:Male; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D014765:Viral Vaccines
D000914:Antibodies, Viral; D007106:Immune Sera; D014765:Viral Vaccines