title: Automated multi-residue isolation of fluoroquinolone antimicrobials from fortified and incurred chicken liver using on-line microdialysis and high-performance liquid chromatography with programmable fluorescence detection.
abstract: Isolation of the quinolones, sarafloxacin (SAR), oxolinic acid (OXA), and flumequine (FMQ), from fortified chicken liver tissues, and SAR incurred chicken liver tissues was achieved by combined liquid-liquid extraction and aqueous on-line microdialysis using the automated trace enrichment of dialysates (ASTED) system. Analysis of tissue isolates after ASTED clean-up was performed using reversed-phase HPLC and programmable fluorescence detection. Overall recoveries of SAR, OXA and FMQ from samples fortified over a concentrations range of 1-100 ppb were 94, 97 and 87% with overall inter-assay variability of 4.2, 4.1 and 3.6%, respectively. Chicken liver samples incurred with SAR at three concentration levels also were tested by the ASTED method. The method exhibited high peak resolution (3.4-4.2 on average), a high signal-to-noise ratio, and demonstrated good precision. The ASTED-HPLC method overall had a lower limit of detection (LOD) of 0.2 ppb, and a limit of quantitation (LOQ) of 1 ppb.
authors: Eastern Regional Research Center, Agricultural Research Service, US Department of Agriculture, Wyndmoor, PA 19038, USA.
authors: E
authors: E
authors: Cohen
authors: R J
authors: RJ
authors: Maxwell
authors: D J
authors: DJ
authors: Donoghue
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002939:Ciprofloxacin / Q000031:analogs & derivatives / Q000032:analysis; D024841:Fluoroquinolones; D008099:Liver / Q000737:chemistry*; D010093:Oxolinic Acid / Q000032:analysis; D011807:Quinolizines / Q000032:analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D011807:Quinolizines; D002939:Ciprofloxacin; D010093:Oxolinic Acid; C047224:sarafloxacin; C012976:flumequine