Comparison of simian virus 40 large T antigen recombinant protein and DNA immunization in the induction of protective immunity from experimental pulmonary metastasis.
In this report we examine the ability of a recombinant tumor antigen preparation to prevent the establishment of experimental pulmonary metastasis. Baculovirus-derived recombinant simian virus 40 (SV40) large tumor antigen (T-Ag) was injected into BALB/c mice followed by challenge with an intravenous injection of syngeneic SV40-transformed tumorigenic cells. The experimental murine pulmonary metastasis model allows for the accurate measurement of metastatic lessions in the lungs at various times after the challenge, using computer-assisted video image analysis. Following challenge, lung metastasis and survival data for the groups of mice were obtained. Animals immunized with recombinant SV40 T-Ag showed no detectable sign of lung metastasis and survived for more than 120 days after challenge. Antibodies specific for SV40 T-Ag were detected in the serum of immunized mice by enzyme-linked immunosorbent assay. Splenocytes obtained from mice immunized with recombinant SV40 T-Ag did not lyse syngeneic tumor cells, indicating that no cytotoxic T lymphocyte response was induced. Control mice developed extensive lung metastasis and succumbed to lethal tumor within 4 weeks after challenge. These data indicate that immunization with the recombinant SV40 T-Ag induces protective, T-Ag-specific immunity in an experimental pulmonary tumor metastasis model.
Watts
A M
AM
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA.
Shearer
M H
MH
Pass
H I
HI
Bright
R K
RK
Kennedy
R C
RC
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D000917:Antibody Formation / Q000276:immunology; D000952:Antigens, Polyomavirus Transforming / Q000276:immunology*; D019496:Cancer Vaccines / Q000276:immunology*; D004195:Disease Models, Animal; D005260:Female; D008175:Lung Neoplasms / Q000276:immunology / Q000517:prevention & control* / Q000556:secondary*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011994:Recombinant Proteins / Q000276:immunology* / Q000627:therapeutic use; D013539:Simian virus 40 / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000952:Antigens, Polyomavirus Transforming; D019496:Cancer Vaccines; D011994:Recombinant Proteins; D019444:Vaccines, DNA