Prostate development requires Sonic hedgehog expressed by the urogenital sinus epithelium.
The prostate gland develops from the urogenital sinus by a testosterone-dependent process of ductal morphogenesis. Sonic hedgehog (Shh) is expressed in the urogenital sinus epithelium and the time course of expression coincides with the formation of the main prostatic ducts. Expression is most abundant in the lumen of the urogenital sinus and in the contiguous proximal duct segments. The initial upregulation of Shh expression in the male urogenital sinus depends on the presence of testosterone. The function of Shh was examined in the male urogenital sinus which was transplanted under the renal capsule of an adult male host mouse. Blockade of Shh function by a neutralizing antibody interferes with Shh signaling and abrogates growth and ductal morphogenesis in the transplanted tissue. These observations show that testosterone-dependent Shh expression in the urogenital sinus is necessary for the initiation of prostate development.
Podlasek
C A
CA
Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Barnett
D H
DH
Clemens
J Q
JQ
Bak
P M
PM
Bushman
W
W
D000818:Animals; D004848:Epithelium / Q000254:growth & development / Q000378:metabolism; D005260:Female; D016220:Fibroblast Growth Factor 1; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D053823:Hedgehog Proteins; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D009024:Morphogenesis / Q000276:immunology; D009924:Organ Culture Techniques; D000072079:Patched Receptors; D011467:Prostate / Q000196:embryology*; D011506:Proteins / Q000276:immunology / Q000502:physiology*; D011956:Receptors, Cell Surface; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000378:metabolism / Q000502:physiology; D013997:Time Factors; D015534:Trans-Activators; D014566:Urogenital System / Q000196:embryology*
D053823:Hedgehog Proteins; D008565:Membrane Proteins; D000072079:Patched Receptors; D011506:Proteins; D011956:Receptors, Cell Surface; D015534:Trans-Activators; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1; D013739:Testosterone