[Cryptic heterokaryons in Saccharomyces cerevisiae strains. Model experiments].
Heterokaryons capable of segregating clones of different phenotypes were obtained in saccharomycetes yeast. Clones expressing phenotypic traits encoded by the nuclear genome of one parent and the mitochondrial genome of another were shown to contain cells that carried the second phenotypically silent nucleus. The cryptic nucleus can be uncovered by growing these "cytoductants" on the corresponding medium. The use of strains carrying an insertion in the URA3 gene and the endogenic plasmid, 2 microns DNA, made it possible to detect this nucleus by blot hybridization.
Nevzgliadova
O V
OV
Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia.
Davydenko
S G
SG
Smirnova
T I
TI
Soĭdla
T R
TR
D002467:Cell Nucleus / Q000235:genetics*; D020090:Chromosome Segregation; D003433:Crosses, Genetic; D004251:DNA Transposable Elements; D004271:DNA, Fungal / Q000235:genetics*; D005819:Genetic Markers; D016681:Genome, Fungal; D015151:Immunoblotting; D008957:Models, Genetic; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D004251:DNA Transposable Elements; D004271:DNA, Fungal; D005819:Genetic Markers