Expression of an engineered form of recombinant procollagen in mouse milk.
We have examined the suitability of the mouse mammary gland for expression of novel recombinant procollagens that can be used for biomedical applications. We generated transgenic mouse lines containing cDNA constructs encoding recombinant procollagen, along with the alpha and beta subunits of prolyl 4-hydroxylase, an enzyme that modifies the collagen into a form that is stable at body temperature. The lines expressed relatively high levels (50-200 micrograms/ml) of recombinant procollagen in milk. As engineered, the recombinant procollagen was shortened and consisted of a pro alpha 2(I) chain capable of forming a triple-helical homotrimer not normally found in nature. Analysis of the product demonstrated that (1) the pro alpha chains formed disulphide-linked trimers, (2) the trimers contained a thermostable triple-helical domain, (3) the N-propeptides were aligned correctly, and (4) the expressed procollagen was not proteolytically processed to collagen in milk.
John
D C
DC
School of Biological Sciences, Wellcome Trust Centre For Cell-Matrix Research, University of Manchester, UK.
Watson
R
R
Kind
A J
AJ
Scott
A R
AR
Kadler
K E
KE
Bulleid
N J
NJ
D000818:Animals; D015152:Blotting, Northern; D015139:Blotting, Southern; D005260:Female; D008321:Mammary Glands, Animal / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D008892:Milk / Q000378:metabolism*; D011347:Procollagen / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D011393:Procollagen-Proline Dioxygenase / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D015202:Protein Engineering; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry
D011347:Procollagen; D011994:Recombinant Proteins; D011393:Procollagen-Proline Dioxygenase