The human homolog of Drosophila cornichon protein is differentially expressed in alloactivated T-cells.
To identify novel genes induced in the early stage of T-cell activation, mRNA expression in alloactivated human lymphocytes was examined. Differential display-reverse transcription PCR analysis revealed a 207-bp cDNA fragment which was upregulated 24 h after allostimulation of a human T-cell line. The corresponding complete 1396 bp cDNA, named TGAM77, encodes a predicted 134 amino acid protein which shares 63% homology with the cornichon (cni) protein of Drosophila melanogaster. Upregulation of TGAM77 mRNA in the early phase of T-cell activation was confirmed by Northern blot and RT-PCR analysis of activated human lymphocytes. TGAM77 mRNA is expressed in a variety of human tissues with various expression levels. In analogy to cni which is involved in an epidermal growth factor-like signaling pathway inducing cellular asymmetry in Drosophila oogenesis, TGAM77 might function in similar signaling establishing vectorial re-localization and concentration of signaling events in T-cell activation.
Utku
N
N
Institut für Medizinische Immunologie, Campus Mitte, Charité, Humboldt Universität, Schumannstrasse 20/21, 10098, Berlin, Germany. nalan.utku@charite.de
Bulwin
G C
GC
Beinke
S
S
Heinemann
T
T
Beato
F
F
Randall
J
J
Schnieders
B
B
Sandhoff
K
K
Volk
H D
HD
Milford
E
E
Gullans
S R
SR
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*
C118276:CNIH1 protein, human; C485558:Cnih protein, mouse; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004527:Egg Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C485557:cni protein, Drosophila