title: [Interbody arthrodesis using a plasmapore titanium block. Mechanical and histological experimental study in sheep].
abstract: A Plasmapore-coated titanium alloy block was implanted in ewes for the purpose of providing interbody fusion. Four blocks were implanted in each ewe: one uncoated block (without Plasmapore) serving as a reference specimen and three blocks coated with Plasmapore. Mechanical testing and histological study were performed on five ewes.
  
  
  OBJECTIVE
  Determination of the quality of the mechanical and histological anchorage of the Plasmapore implanted in the interbody space in the same animal living under conditions of physical strain exerted on the spine.
  
  
  METHODS
  Four months after surgical implantation of the block, the ewes were sacrificed: the removed spines were frozen for subsequent mechanical analysis and preserved in a solution of 40 degrees alcohol for subsequent histological analysis. X-rays were taken to evaluate the positioning of the implant. The mechanical analysis included extraction tests, measuring the maximum extraction force and evaluating the stiffness of the system, being indicative of the mechanical fixation quality. The histological study included both qualitative and quantitative analysis, together with an evaluation of the osteointegration of the blocks coated with Plasmapore.
  
  
  RESULTS
  After 4 months of implantation, a mean extraction force of 990 N was necessary for the blocks coated with Plasmapore, and of 1.338 N for the blocks coated with Plasmapore with additional osteosynthesis, whereas a mean extraction force of 332 N was necessary for the uncoated blocks. Anchorage and resistance against uprooting of the blocks coated with Plasmapore were significantly more efficient. The histological study revealed the presence of bone neoformation adhering to the implant. Quantification of this bone formation covering nearly 45% of the implant perimeter, confirmed both osteointegration of the implant perimeter, confirmed both osteointegration of the implant surfaces being in contact with the vertebral endplates and osteoconduction along the lateral surfaces.
  
  
  CONCLUSIONS
  The titanium Plasmapore block enables interbody fusion due to an osteointegration of the vertebral endplates by Plasmapore coating, which was proved by the results of extraction testing and histological study. It should be taken into account that no additional bone grafts have been used and that the implant had not been forced into the spongiosa.
authors: Centre hospitalo-universitaire, service d'orthopédie, traumatologie, chemin des Bourrelys, Marseille, France.
authors: P
authors: P
authors: Tropiano
authors: A
authors: A
authors: Diop
authors: J
authors: J
authors: Dejou
authors: J J
authors: JJ
authors: Bronsard
authors: D G
authors: DG
authors: Poitout
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000033:anatomy & histology; D005069:Evaluation Studies as Topic; D020007:Implants, Experimental; D016348:Osseointegration; D012756:Sheep; D013123:Spinal Fusion / Q000379:methods*; D013997:Time Factors; D014025:Titanium
chemical_list: D014025:Titanium