title: Uncapping and deregulation of telomeres lead to detrimental cellular consequences in yeast.
abstract: Telomeres are the protein-nucleic acid structures at the ends of eukaryote chromosomes. Tandem repeats of telomeric DNA are templated by the RNA component (TER1) of the ribonucleoprotein telomerase. These repeats are bound by telomere binding proteins, which are thought to interact with other factors to create a higher-order cap complex that stabilizes the chromosome end. In the budding yeast Kluyveromyces lactis, the incorporation of certain mutant DNA sequences into telomeres leads to uncapping of telomeres, manifested by dramatic telomere elongation and increased length heterogeneity (telomere deregulation). Here we show that telomere deregulation leads to enlarged, misshapen "monster" cells with increased DNA content and apparent defects in cell division. However, such deregulated telomeres became stabilized at their elongated lengths upon addition of only a few functionally wild-type telomeric repeats to their ends, after which the frequency of monster cells decreased to wild-type levels. These results provide evidence for the importance of the most terminal repeats at the telomere in maintaining the cap complex essential for normal telomere function. Analysis of uncapped and capped telomeres also show that it is the deregulation resulting from telomere uncapping, rather than excessive telomere length per se, that is associated with DNA aberrations and morphological defects.
authors: Department of Microbiology & Immunology, University of California, San Francisco, San Francisco, CA 94143-0414, USA.
authors: C D
authors: CD
authors: Smith
authors: E H
authors: EH
authors: Blackburn
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D002455:Cell Division; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005434:Flow Cytometry; D007716:Kluyveromyces / Q000166:cytology* / Q000235:genetics* / Q000254:growth & development; D008954:Models, Biological; D016601:RNA-Binding Proteins / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000235:genetics / Q000502:physiology*
chemical_list: D004271:DNA, Fungal; D004268:DNA-Binding Proteins; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; D019098:Telomerase