Structural features of the interaction between an anti-clonotypic antibody and its cognate T-cell antigen receptor.
The crystal structure of the complex between a single chain Fv fragment of the KB5-C20 T-cell antigen receptor (TCR) and the specific anti-clonotypic antibody (Ab) Désiré-1 provides the first description of the interface between a clonotype and an anti-clonotype. In the four idiotype/anti-idiotype complexes of known three-dimensional structures, the interacting Fv fragments associate largely through their complementarity-determining regions (CDRs). In marked contrast, Désiré-1 binds to a face of the KB5-C20 TCR that is almost perpendicular to the TCR antigen binding site, and recognizes discontinuous stretches of TCR Valpha and Vbeta residues that belong to both the CDRs and the framework. Despite this peculiar mode of interaction, Désiré-1 constitutes a genuine anti-clonotypic Ab. Moreover, in spite of the fact that the Désiré-1 contact residues do not constitute a molecular mimic of the physiological ligand normally recognized by the KB5-C20 TCR, the bivalent Désiré-1 Ab is capable of efficiently activating T-cells expressing the KB5-C20 TCR.
Mazza
G
G
Centre d'Immunologie, INSERM-CNRS de Marseille-Luminy, Marseille Cedex 9, 13288, France.
Housset
D
D
Piras
C
C
Grégoire
C
C
Fontecilla-Camps
J C
JC
Malissen
B
B
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D018360:Crystallography, X-Ray; D008213:Lymphocyte Activation; D051379:Mice; D008958:Models, Molecular; D011487:Protein Conformation; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014867:Water / Q000737:chemistry
D000888:Antibodies, Anti-Idiotypic; D011948:Receptors, Antigen, T-Cell; D014867:Water