Modulation of rat preadipocyte adipose conversion by androgenic status: involvement of C/EBPs transcription factors.
Androgenic status affects rat preadipocyte adipose conversion from two deep intra-abdominal (epididymal and perirenal) fat depots differently. The aim of this study was to establish whether these site-specific alterations of adipogenesis are related to altered expressions of the transcriptional factors regulating proliferation and differentiation of preadipocytes, c-myc and CCAAT/enhancer binding proteins (C/EBPs: C/EBPalpha and beta). The increased proliferation of epididymal and perirenal preadipocytes from castrated rats was not linked to variations in c-myc mRNA and protein levels. The expression of the early marker of adipogenesis, lipoprotein lipase (LPL), was decreased by androgenic deprivation in epididymal cells but remained insensitive to the androgenic status in perirenal preadipocytes. In contrast, LPL expression increased in subcutaneous preadipocytes from castrated rats, an effect which was partly corrected by testosterone treatment. Expression of C/EBPbeta was unaffected by androgenic status whatever the anatomical origin of the preadipocytes. In contrast, the mRNA and protein levels of C/EBPalpha were greatly decreased by androgenic deprivation in epididymal cells, an alteration which could not be corrected by in vivo testosterone administration. Altogether these results demonstrated that in preadipocytes androgenic deprivation affects site-specifically the expression of LPL, an early marker of adipogenesis and of C/EBPalpha, a master regulator of adipogenesis. These observations contribute to an explanation of why castration induces defective adipose conversion in rat epididymal preadipocytes specifically.
Garcia
E
E
INSERM CJF 94-02, Faculté de Médecine Paris-Ouest, Université René Descartes, Paris V, Centre Hospitalier de Poissy, Poissy 78303, France.
Lacasa
M
M
Agli
B
B
Giudicelli
Y
Y
Lacasa
D
D
D017667:Adipocytes / Q000502:physiology*; D000728:Androgens / Q000502:physiology*; D000818:Animals; D015415:Biomarkers; D015152:Blotting, Northern; D015153:Blotting, Western; D022762:CCAAT-Enhancer-Binding Proteins; D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004822:Epididymis; D015870:Gene Expression / Q000187:drug effects; D007668:Kidney; D008071:Lipoprotein Lipase / Q000235:genetics; D008297:Male; D009687:Nuclear Proteins / Q000378:metabolism*; D009919:Orchiectomy; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D017207:Rats, Sprague-Dawley; D012269:Ribosomal Proteins / Q000235:genetics; D013234:Stem Cells / Q000502:physiology*; D013739:Testosterone / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism*
D000728:Androgens; D015415:Biomarkers; D022762:CCAAT-Enhancer-Binding Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012269:Ribosomal Proteins; D014157:Transcription Factors; D013739:Testosterone; D008071:Lipoprotein Lipase