Application of bis-eugenol to a zinc oxide eugenol cement.
OBJECTIVE
To assess the usefulness of dimerized eugenol (bis-eugenol) in dentistry, the physical properties of zinc oxide eugenol cement (ZOE) with bis-eugenol and the cytotoxicity of bis-eugenol were studied.


METHODS
Setting time, compressive strength, solubility and disintegration of ZOE cement with bis-eugenol according to the specifications of JDMAS315 were evaluated. The cytotoxicity of bis-eugenol and eugenol toward two different cell types, HGF (a primary culture of human gingival fibroblast) and HSG (a human epidermoid carcinoma cell line derived from a salivary gland) was evaluated by the MTT test and in terms of cell survival.


RESULTS
Addition of bis-eugenol to ZOE did not decrease the physical properties when employed at the ratio of 9:1 or 6:1 (liquid ND:bis-eugenol, w/w). Bis-eugenol was less toxic than eugenol in the cell culture tests.


CONCLUSIONS
The results of this assay demonstrated that bis-eugenol is useful in ZOE.
Fujisawa
S
S
Department of Oral Diagnosis, Meikai University, School of Dentistry, Saitama-ken, Japan. fujisawa@dent.meikai.ac.jp
Kashiwagi
Y
Y
Atsumi
T
T
Iwakura
I
I
Ueha
T
T
Hibino
Y
Y
Yokoe
I
I
D000230:Adenocarcinoma; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D005054:Eugenol / Q000737:chemistry* / Q000633:toxicity; D005347:Fibroblasts / Q000187:drug effects; D005881:Gingiva / Q000166:cytology / Q000187:drug effects; D006801:Humans; D012468:Salivary Gland Neoplasms; D014407:Tumor Cells, Cultured; D015035:Zinc Oxide-Eugenol Cement / Q000737:chemistry* / Q000633:toxicity
D004338:Drug Combinations; D015035:Zinc Oxide-Eugenol Cement; D005054:Eugenol