title: Induction of protein-like molecular architecture by self-assembly processes.
abstract: One of the most intriguing self-assembly processes is the folding of peptide chains into native protein structures. We have developed a method for building protein-like structural motifs that incorporate sequences of biological interest. A lipophilic moiety is attached onto an N(alpha)-amino group of a peptide chain, resulting in a 'peptide-amphiphile'. The alignment of amphiphilic compounds at the lipid solvent interface is used to facilitate peptide alignment and structure initiation and propagation. Peptide-amphiphiles containing potentially triple-helical structural motifs have been synthesized. The resultant head group structures have been characterized by circular dichroism and NMR spectroscopies. Evidence for a self-assembly process of peptide-amphiphiles has been obtained from: (a) circular dichroism spectra and melting curves characteristic of triple-helices, (b) one- and two-dimensional NMR spectra indicative of stable triple-helical structure at low temperatures and melted triple-helices at high temperatures, and (c) pulsed-field gradient NMR experiments demonstrating different self-diffusion coefficients between proposed triple-helical and non-triple-helical species. The peptide-amphiphiles described here provide a simple approach for building stable protein structural motifs using peptide head groups.
authors: Department of Chemistry & Biochemistry and the Center for Molecular Biology & Biotechnology, Florida Atlantic University, Boca Raton 33431, USA.
authors: G B
authors: GB
authors: Fields
mesh_terms: D002942:Circular Dichroism; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary; D011506:Proteins / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry; D013696:Temperature
chemical_list: D010455:Peptides; D011506:Proteins; D013501:Surface-Active Agents