title: Detection of Chlamydia pneumoniae DNA in atheromatous tissues by polymerase chain reaction.
abstract: Chlamydia pneumoniae is an important human respiratory pathogen. Recently, seroepidemiological data and the demonstration of chlamydial DNA or antigen within parts of atherosclerotic lesions have suggested a causal association between chlamydial infections and atherosclerosis. As the results obtained by different groups show considerable variations, we provide further data based on a highly specific and sensitive nested PCR method. Positivity was confirmed by nonradioactive hybridization with a specific probe, and sensitivity was determined by titration experiments. C. pneumoniae DNA was detected in 8/29 (28%) of carotid artery samples and 3/14 (21%) of aortic aneurysms.
authors: Klinische Abteilung für Klinische Mikrobiologie des Klinischen Institutes für Hygiene, Vienna, Austria.
authors: R
authors: R
authors: Nadrchal
authors: A
authors: A
authors: Makristathis
authors: P
authors: P
authors: Apfalter
authors: M
authors: M
authors: Rotter
authors: W
authors: W
authors: Trubel
authors: I
authors: I
authors: Huk
authors: P
authors: P
authors: Polterauer
authors: A M
authors: AM
authors: Hirschl
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001161:Arteriosclerosis / Q000473:pathology* / Q000601:surgery; D002339:Carotid Arteries / Q000473:pathology; D016893:Carotid Stenosis / Q000473:pathology* / Q000601:surgery; D002690:Chlamydia Infections / Q000473:pathology*; D016993:Chlamydophila pneumoniae; D004269:DNA, Bacterial / Q000032:analysis*; D016894:Endarterectomy, Carotid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial