[Binding of transcription factors with functionally significant regions of MDR1 gene promoter during its activation].
Interaction of transcription factors with functional regions of gene mdr1 promoter has been studied. Binding of transcription factors to double-stranded (ds) oligonucleotides, mimicking four important regulatory regions before and after treatment of KB and K-562 cell lines with doxorubicin, cytarabine, and vinblastine was analysed by gel-shift assay. Gene induction resulted in enhancement of the main complex A(A1) proteins formation on the all four regions in both the cell lines. Inhibition of each complex formation by an excess of non-radiolabelled ds oligonucleotides suggests the possibility of regions interaction during promoter activation. Another indication of interaction of regulatory regions during activation is overlapping minor complexes sets. The results suggest that the four studied regulatory regions are important for mdr1 gene activation. Corresponding oligonucleotides mimicking these regions can be employed as inhibitors of gene transcription that bind specific transcriptional factors.
Bozhenok
L N
LN
Institute of Bioorganic Chemistry, Siberian Branch of Russian Academy of Science, Novosibirsk.
Kolpakova
A V
AV
Vladimirova
A V
AV
Chernolovskaia
E L
EL
Vlasov
V V
VV
D001483:Base Sequence; D018432:Drug Resistance, Multiple / Q000235:genetics*; D005786:Gene Expression Regulation; D006801:Humans; D020014:K562 Cells; D007624:KB Cells; D008969:Molecular Sequence Data; D015345:Oligonucleotide Probes; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*; D015533:Transcriptional Activation
D015345:Oligonucleotide Probes; D014157:Transcription Factors