Analysis of immunomodulating cytokine mRNAs in the mouse induced by mushroom polysaccharides.
The immunomodulating action of two mushroom antitumor polysaccharides, polysaccharide-protein complex (PSPC) and lentinan, was elucidated through analysing the expression profile of cytokines during a time course (0 h to 48 h) after their administration. Among the 5 cytokine genes, the induction of a marked increase in the mRNA levels of IL-1alpha, IL-1beta, TNF-alpha, IFN-gamma and M-CSF by PSPC and lentinan was observed in the peritoneal exudate cells and splenocytes. However, the time point of their increased production was different after PSPC and lentinan administration.
Liu
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Department of Microbiology, Nankai University, Tianjin, China.
Ooi
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Fung
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MC
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000363:Agaricales / Q000502:physiology*; D000818:Animals; D016207:Cytokines / Q000235:genetics*; D007375:Interleukin-1 / Q000235:genetics; D007912:Lentinan / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011134:Polysaccharides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D000276:Adjuvants, Immunologic; D016207:Cytokines; D007375:Interleukin-1; D011134:Polysaccharides; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D007912:Lentinan