A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2.
Recently a number of nonclass I genes were discovered in the human MHC class I region. One of these, FAT10, encodes a protein consisting of two domains with homology to ubiquitin. FAT10 mRNA is expressed constitutively in some lymphoblastoid lines and dendritic cells and in certain other cells after gamma-interferon induction. FAT10 protein expression is controlled at several levels including transcription, translation, and protein stability. Yeast two-hybrid screening of a human lymphocyte library and immunoprecipitation studies revealed that FAT10 noncovalently associated with MAD2, a protein implicated in a cell-cycle checkpoint for spindle assembly during anaphase. Thus, FAT10 may modulate cell growth during B cell or dendritic cell development and activation.
Liu
Y C
YC
Department of Genetics, Internal Medicine, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06511, USA.
Pan
J
J
Zhang
C
C
Fan
W
W
Collinge
M
M
Bender
J R
JR
Weissman
S M
SM
D000595:Amino Acid Sequence; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001483:Base Sequence; D019556:COS Cells; D002135:Calcium-Binding Proteins; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D018797:Cell Cycle Proteins; D002460:Cell Line; D018244:Chromosomes, Artificial, Yeast; D005805:Genes, MHC Class I; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D064246:Mad2 Proteins; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013601:T-Lymphocytes / Q000276:immunology; D014162:Transfection; D014407:Tumor Cells, Cultured; D014452:Ubiquitins / Q000737:chemistry
D002135:Calcium-Binding Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; C577697:MAD2L1 protein, human; D064246:Mad2 Proteins; D012097:Repressor Proteins; C109184:UBD protein, human; D014452:Ubiquitins