Modifying secretion and post-translational processing in insect cells.
The baculovirus-insect cell system is a valuable tool for the expression of heterologous proteins. Due to limitations in the intracellular processing environment, however, heterologous secreted and membrane proteins are often insoluble, poorly processed, or contain 'non-human' modifications. Recent attempts to modify the insect cell secretory pathway by overexpressing processing factors have demonstrated the potential to overcome these limitations.
Ailor
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Department of Chemical Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Betenbaugh
M J
MJ
D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D003001:Cloning, Molecular; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D007313:Insecta; D018832:Molecular Chaperones; D009844:Oligosaccharides / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D021382:Protein Sorting Signals / Q000378:metabolism
D018832:Molecular Chaperones; D009844:Oligosaccharides; D021382:Protein Sorting Signals