title: Promotion of antibiotic production by high ethanol, high NaCl concentration, or heat shock in Pseudomonas fluorescens S272.
abstract: A stress imposed by a continuous feed of high ethanol, high NaCl concentration, or a high temperature shock increased antibiotic production by several times in Pseudomonas fluorescens S272. A tentative bioassay showed that the stress caused about 40-fold elevation in the autoinducer activity. Addition of synthetic autoinducers, N-(3-oxododecanoyl)-L-homoserine lactone or N-(3-oxohexanoyl)-L-homoserine lactone at a concentration of more than 100 micrograms/l to a non-stressed culture also increased the antibiotic production by several times. These results suggested that the antibiotic production in P. fluorescens S272 was regulated by N-acyl-homoserine lactone and the promotive effect by stress occurred through any function that increased the autoinducer production.
authors: Central Research Laboratories, Mercian Corporation, Fujisawa, Japan. knakata@mb.infoweb.ne.jp
authors: K
authors: K
authors: Nakata
authors: A
authors: A
authors: Yoshimoto
authors: Y
authors: Y
authors: Yamada
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000032:analysis / Q000096:biosynthesis*; D002855:Chromatography, Thin Layer; D000431:Ethanol / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response / Q000502:physiology; D010636:Phenols; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D011551:Pseudomonas fluorescens / Q000254:growth & development / Q000378:metabolism*; D011758:Pyrroles; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D010636:Phenols; D011758:Pyrroles; C095503:pyoluteorin; C088386:homoserine lactone; D000431:Ethanol; D012965:Sodium Chloride; C059817:2,4-diacetylphloroglucinol; D010696:Phloroglucinol; D015107:4-Butyrolactone