title: Histopathological pattern and humoral immune response to a crude exo-antigen and purified keratinase of Microsporum canis in symptomatic and asymptomatic infected cats.
abstract: In order to understand better the mechanisms involved in the diverse clinical patterns in Microsporum canis-infected cats, the histopathological features were compared in symptomatic and asymptomatic infected cats. Additionally, the IgG immune response to a crude exo-antigen and purified keratinase of M. canis was studied by ELISA in cats of various clinical and mycological status. Acute and subacute perifolliculitis and folliculitis occurred more frequently in symptomatic than asymptomatic cats. The latter usually displayed signs of chronic inflammation and a marked infiltration of superficial dermis by mast cells, which would suggest that these animals present similarities to chronically dermatophytic humans or animals. When using a crude M. canis antigen, all infected cats were shown to have significantly higher levels of specific IgG when compared to culture negative and mechanical carrier-cats. In these non-infected animals, specific IgG was more frequently detected in adults than in young animals. No difference in anti-crude antigen specific IgG was observed between symptomatic and asymptomatic infected cats, indicating that the presence of IgG is probably unrelated to the clinical status of cats. Anti-keratinase specific IgG was only detected in one of the infected cats.
authors: University of Liège, Faculty of Veterinary Medicine, Department of Parasitology and Parasitic Diseases, Belgium. bmignon@ulg.ac.be
authors: B R
authors: BR
authors: Mignon
authors: F
authors: F
authors: Coignoul
authors: T
authors: T
authors: Leclipteux
authors: C
authors: C
authors: Focant
authors: B J
authors: BJ
authors: Losson
mesh_terms: D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood*; D000946:Antigens, Fungal / Q000276:immunology; D002371:Cat Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D002415:Cats; D003881:Dermatomycoses / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D008297:Male; D008864:Microsporum / Q000201:enzymology / Q000276:immunology*; D010447:Peptide Hydrolases / Q000276:immunology*
chemical_list: D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D010447:Peptide Hydrolases; C035855:keratinase