Determination of ferrocene-A in the blood serum of rabbits using reversed-phase microcolumn HPLC.
Ferrocene-A (ferrocenyl-1-phenyl-1-dioxy-1,4-butin-2) has shown significant antitumour and antibacterial activity. To facilitate studies in this field a simple and rapid liquid chromatographic procedure was developed, allowing the determination of ferrocene-A in the blood serum. The procedure was based on a reversed-phase high-performance liquid chromatography (HPLC) method, with UV detection at 210 nm. The mobile phase ethanol-0.01 M KH2PO4 60:40 (v/v) provides the complete separation of ferrocene-A, internal standard and endogenous compounds of serum. The recovery of ferrocene-A from serum using a hexaneisoamyl alcohol mixture was 90%. The proposed method is convenient for pharmacological and pharmacokinetic applications.
Pochkhidze
M Sh
MSh
Department of Chemistry, Tbilisi State University, Republic of Georgia.
Asatiani
L P
LP
Rukhadze
M D
MD
Chitiashvili
Z J
ZJ
Tsartsidze
M A
MA
D000818:Animals; D000890:Anti-Infective Agents / Q000097:blood*; D000970:Antineoplastic Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005296:Ferrous Compounds / Q000097:blood*; D000075163:Metallocenes; D011817:Rabbits; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D000890:Anti-Infective Agents; D000970:Antineoplastic Agents; D005296:Ferrous Compounds; D000075163:Metallocenes; C004998:ferrocene