Sequence analysis of the four plasmid genes required to produce the circular peptide antibiotic microcin J25.
A 4.8-kb plasmid region carrying the four genes mcjABCD necessary for production of and immunity to the cyclic peptide antibiotic microcin J25 (MccJ25) has been sequenced. mcjA encodes the primary structure of MccJ25 as a precursor endowed with an N-terminal extension of 37 amino acids. The products of mcjB and mcjC are thought to be involved in microcin maturation, which implies cleavage of McjA and head-tail linkage of the 21-residue pro-MccJ25. The predicted McjD polypeptide, which is highly similar to several ABC exporters, was found to be required for MccJ25 secretion, thus explaining its ability to confer immunity to MccJ25.
Solbiati
J O
JO
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas (Consejo Nacional de Investigaciones Científicas y Técnicas-Universidad Nacional de Tucumán), Argentina.
Ciaccio
M
M
Farías
R N
RN
González-Pastor
J E
JE
Moreno
F
F
Salomón
R A
RA
D018528:ATP-Binding Cassette Transporters / Q000235:genetics; D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D010957:Plasmids / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D018528:ATP-Binding Cassette Transporters; D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D010456:Peptides, Cyclic; D011498:Protein Precursors; C020411:microcin