Human ileostomy glycoproteins as a model for small intestinal mucus to investigate adhesion of probiotics.
Human ileostomy glycoproteins were used as a model for small intestinal mucus to investigate the adhesion of 12 Lactobacillus strains, one Lactococcus strain and one Propionibacterium strain, Both probiotic and dairy strains were tested. Adhesive and non-adhesive Escherichia coli strains were used as controls. All the strains were also tested for their adhesion to polystyrene. Adhesion to ileostomy glycoproteins and to polystyrene varied significantly among the strains tested. Lactobacillus rhamnosus (human isolate), Lactobacillus GG, Lact. acidophilus 1 and P. freudenreichii adhered to ileostomy glycoproteins. Adhesion was concentration-dependent and the most adhesive strains were able to saturate the substratum. These results indicate that human ileostomy glycoproteins can be used as a model system to select potential probiotic strains to complement the adhesion test with intestinal cell lines.
Tuomola
E M
EM
Department of Biochemistry and Food Chemistry, University of Turku, Finland. elina.tuomola@utu.fi
Ouwehand
A C
AC
Salminen
S J
SJ
D001422:Bacterial Adhesion / Q000187:drug effects*; D018938:Caco-2 Cells; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000502:physiology; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006094:Gram-Positive Bacteria / Q000502:physiology*; D006801:Humans; D007081:Ileostomy; D007422:Intestines / Q000737:chemistry* / Q000382:microbiology; D009093:Mucus; D011137:Polystyrenes; D019936:Probiotics
D006023:Glycoproteins; D011137:Polystyrenes