In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II.
A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.
Juretschko
S
S
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
Schönhuber
W
W
Kulakauskas
S
S
Ehrlich
D S
DS
Schleifer
K H
KH
Amann
R
R
D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D017404:In Situ Hybridization, Fluorescence; D015345:Oligonucleotide Probes; D010957:Plasmids; D012313:RNA / Q000235:genetics*
D015345:Oligonucleotide Probes; C034477:RNA primers; D012313:RNA