Detection of the source of mislabeled biopsy tissue paraffin block and histopathological section on glass slide.
The aim of this study was to identify the source of surgical biopsy tissue in paraffin block and histochemically stained biopsy section on a slide suspected to be mislabeled. Commercially available polymerase chain reaction (PCR)-based human lymphocyte antigen (HLA) DQA1 and Polymarker kits (Roche Molecular Systems, Branchburg, NJ, U.S.A.). were used to generate DNA profiles from biopsy tissue material in paraffin block and on histological slide, and the reference blood sample was collected from the patient. The source of biopsy tissue was detected by HLA DQA1 and polymarker profiling. Profiles obtained from biopsy material were consistent with those obtained from reference blood sample. The PCR-based DNA profiling techniques can determine the source of minute tissue in paraffin block and stained tissue sections on slides routinely prepared for diagnosis of carcinoma.
Giroti
R
R
DNA Typing Unit, Central Forensic Science Laboratory, Calcultta, India.
Kashyap
V K
VK
D015415:Biomarkers; D001706:Biopsy; D001943:Breast Neoplasms / Q000175:diagnosis / Q000473:pathology*; D002277:Carcinoma / Q000175:diagnosis / Q000473:pathology*; D004247:DNA / Q000097:blood; D003951:Diagnostic Errors; D005260:Female; D005568:Forms and Records Control; D006021:Glycophorins / Q000235:genetics; D006683:HLA-DQ Antigens / Q000235:genetics; D059848:HLA-DQ alpha-Chains; D006454:Hemoglobins / Q000235:genetics; D006801:Humans; D008875:Middle Aged; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D011973:Receptors, LDL / Q000235:genetics; D013048:Specimen Handling / Q000379:methods*; D014809:Vitamin D-Binding Protein / Q000235:genetics
D015415:Biomarkers; D006021:Glycophorins; D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen; D006454:Hemoglobins; D011973:Receptors, LDL; D014809:Vitamin D-Binding Protein; D004247:DNA