Permeation of several drugs through keratinized epithelial-free membrane of hamster cheek pouch.
The hamster cheek pouch mucosa was selected as a substitute for the human buccal mucosa in an in vitro permeation study. Considering that a keratinized layer is not present in the human buccal mucosa, keratinized epithelial-free hamster cheek pouch (KEF-membrane) was prepared by chemical splitting. To confirm the usefulness of the KEF-membrane, a permeation study was conducted using several drugs with different lipophilicities. The permeability coefficient of hydrophilic drugs through the KEF-membrane (Pkef) was significantly greater than that through a viable KEF-membrane (Pkef-viable), which was estimated by using the permeability coefficient of the viable full-thickness membrane and that of the keratinized layer. On the other hand, the Pkef values of lipophilic drugs were comparable with the Pkef-viable values. Furthermore, the ratio of these P values (Pkef/Pkef-viable) decreased with increasing lipophilicity of drugs. These findings indicate that the KEF-membrane may be useful for buccal permeation studies of lipophilic drugs.
Tsutsumi
K
K
Department of Pharmaceutics, Hoshi University, Tokyo, Japan.
Obata
Y
Y
Takayama
K
K
Isowa
K
K
Nagai
T
T
D000818:Animals; D002610:Cheek; D006224:Cricetinae; D004848:Epithelium / Q000378:metabolism; D066298:In Vitro Techniques; D007633:Keratins / Q000378:metabolism*; D008297:Male; D009061:Mouth Mucosa / Q000378:metabolism*; D010539:Permeability; D012995:Solubility
D007633:Keratins