Bovine herpesvirus 1-induced apoptotic cell death: role of glycoprotein D.
Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in peripheral blood mononuclear cells and in bovine B lymphoma (BL-3) cells. Attachment but not penetration of BHV-1 is necessary to induce apoptosis in target cells, suggesting that one or more BHV-1 envelope glycoproteins could be involved in the activation of the apoptotic process. In the present study, we demonstrate that, although BHV-1 virions devoid of glycoprotein D (BHV-1 gD-/-) still bind to BL-3 cells, they are no longer able to induce apoptosis. In contrast, virions that contain glycoprotein D (gD) in the viral envelope but do not genetically encode gD (BHV-1 gD-/+) induce a level of apoptosis comparable to that produced by wild-type (wt) BHV-1. In addition, monoclonal antibodies directed against gD, but not against gB or gC, strongly reduced the high levels of apoptosis induced by BHV-1. These observations demonstrate that the induction of apoptosis is directly due to BHV-1 viral particles harboring gD in the viral envelope. Interestingly, binding of affinity-purified gD to BL-3 cells did not induce apoptosis but inhibited the ability of wt BHV-1 to induce apoptosis. Altogether, these results provide evidence for the direct or indirect involvement of gD in the mechanism by which BHV-1 induces apoptosis.
Hanon
E
E
Faculty of Veterinary Medicine, University of Liège, Liège, B-4000, Belgium.
Keil
G
G
van Drunen Littel-van den Hurk
S
S
Griebel
P
P
Vanderplasschen
A
A
Rijsewijk
F A
FA
Babiuk
L
L
Pastoret
P P
PP
D000818:Animals; D000911:Antibodies, Monoclonal; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D017209:Apoptosis / Q000276:immunology*; D002417:Cattle; D005434:Flow Cytometry; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D007136:Immunoglobulins / Q000235:genetics / Q000276:immunology*; D014407:Tumor Cells, Cultured; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000956:Antigens, Viral; D007136:Immunoglobulins; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins