title: Synthetic peptide antigens induce antibodies to Taenia ovis oncospheres.
abstract: Sheep immunised with the Taenia ovis recombinant 45W antigen are protected from infection with the parasite. Two peptides were synthesised corresponding to putative host-protective regions at the N- and C-termini of 45W. Sera from sheep immunised with 45W or related recombinant proteins reacted strongly with the N-terminal peptide. Approximately 40% of the antibody directed against 45WB/X, a truncated form of 45W, was found to be directed against the N-terminal peptide sequence. Sheep were immunised with the N- and C-terminal peptides alone or conjugated to a carrier protein. The N-terminal peptide was found to be highly immunogenic whereas the C-terminal peptide required conjugation to a carrier protein to be immunogenic. Antibodies raised against each of these immunogens crossreacted with the parent protein, 45WB/X, however, only antibodies specific for the N-terminal peptide were found to bind to antigens from the T. ovis oncosphere.
authors: Department of Microbiology and Immunology, University of Melbourne, Parkville, Vic., Australia.
authors: D L
authors: DL
authors: Dadley-Moore
authors: M W
authors: MW
authors: Lightowlers
authors: J S
authors: JS
authors: Rothel
authors: D C
authors: DC
authors: Jackson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000947:Antigens, Helminth / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid / Q000662:veterinary; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D008213:Lymphocyte Activation / Q000276:immunology; D008969:Molecular Sequence Data; D011994:Recombinant Proteins / Q000276:immunology; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology / Q000517:prevention & control*; D013621:Taenia / Q000276:immunology*; D013622:Taeniasis / Q000517:prevention & control*; D014611:Vaccination / Q000662:veterinary*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic