title: Microvascular perfusion deficits are not a prerequisite for mucosal injury in septic rats.
abstract: Our major objective was to investigate whether injury to the mucosa of the small intestine occurred in a normotensive model of sepsis and whether such injury was associated with microvascular perfusion deficits. Using fluorescence intravital microscopy, we show direct evidence of cell injury within the mucosa (pneumonia 12.4 +/- 2.6 cells/field, sham 2.2 +/- 0.7 cells/field), whereas use of 51Cr-labeled EDTA showed evidence of increased mucosal permeability (pneumonia 1.90 +/- 0.67 ml. min-1. 100 g-1; sham 0.24 +/- 0.04 ml. min-1. 100 g-1), 48 h following induction of pneumonia. Despite such injury the capillary density in the ileal mucosa and submucosa of pneumonic rats (1,027 +/- 77 and 1,717 +/- 86 mm2) was not significantly different compared with sham (998 +/- 63 and 1,812 +/- 101 mm2). However, a modest albeit significant decrease in capillary perfusion was measured in the muscularis layer of pneumonia (11.0 +/- 1.3 mm) compared with sham (13.9 +/- 0.63 mm) and appeared to be associated with leukocyte entrapment. Pretreatment using low doses of endotoxin to induce endotoxin tolerance not only increased muscularis capillary density but reduced the number of leukocytes trapped within the microvasculature, decreased myeloperoxidase activity within the ileum in pneumonic rats, and prevented mucosal injury. In conclusion, we have shown that pneumonia results in remote injury to the mucosa of the ileum and that such injury was not associated with concurrent mucosal perfusion deficits.
authors: London Health Sciences Centre Research, and the Departments of Medical Biophysics and Medicine, University of Western Ontario, London, Ontario, Canada N6A 4G5.
authors: R R
authors: RR
authors: Nevière
authors: M L
authors: ML
authors: Pitt-Hyde
authors: R D
authors: RD
authors: Piper
authors: W J
authors: WJ
authors: Sibbald
authors: R F
authors: RF
authors: Potter
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000473:pathology / Q000503:physiopathology*; D007413:Intestinal Mucosa / Q000098:blood supply* / Q000473:pathology* / Q000503:physiopathology; D007421:Intestine, Small / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008833:Microcirculation / Q000473:pathology / Q000503:physiopathology*; D009195:Peroxidase / Q000032:analysis; D018410:Pneumonia, Bacterial / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D011552:Pseudomonas Infections / Q000473:pathology / Q000503:physiopathology*; D011550:Pseudomonas aeruginosa; D051381:Rats; D011917:Rats, Inbred Lew
chemical_list: D009195:Peroxidase