Different effects of the constituents of EGb761 on apoptosis in rat cerebellar granule cells induced by hydroxyl radicals.
The present study was conducted to evaluate the different effects of the constituents of EGb761 (Ginkgo biloba Extract) on apoptosis in cerebellar granule cells induced by hydroxyl radicals. The total flavonoid component of EGb761, two pure EGb761 components (rutin and quercetin), and a mixture of flavonoids and terpenes protected cerebellar granule cells from oxidative damage and apoptosis induced by hydroxyl radicals. ESR(electron spin resonance) results showed that the IC50 of the flavonoids for scavenging hydroxyl radicals was almost the same as that of EGb761, even though flavonoids make up only 24% of EGb761, implying that other constituents of EGb761 besides flavonoids can scavenge hydroxyl radicals. Total terpenes of EGb761 did not protect against apoptosis. Flavonoids and terpenes did not show a synergistic effect in this regard. Terpenes did not scavenge hydroxyl radicals directly, which might be related to their "cage-like" structures.
Chen
C
C
Institute of Biophysics, Academia Sinica, Beijing, P. R. China.
Wei
T
T
Gao
Z
Z
Zhao
B
B
Hou
J
J
Xu
H
H
Xin
W
W
Packer
L
L
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002531:Cerebellum / Q000187:drug effects* / Q000648:ultrastructure; D053938:DNA Fragmentation; D004578:Electron Spin Resonance Spectroscopy; D005415:Flavins / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology*; D020441:Ginkgo biloba / Q000737:chemistry; D017665:Hydroxyl Radical; D008854:Microscopy, Electron; D010936:Plant Extracts; D010946:Plants, Medicinal; D011794:Quercetin / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012431:Rutin / Q000494:pharmacology; D013729:Terpenes / Q000494:pharmacology; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D000096983:Ginkgo Extract
D005415:Flavins; D005419:Flavonoids; D017665:Hydroxyl Radical; D010936:Plant Extracts; D011794:Quercetin; D012431:Rutin; D013729:Terpenes; D017392:Thiobarbituric Acid Reactive Substances; C063170:Ginkgo biloba extract; D000096983:Ginkgo Extract