title: Biochemical and immunogenetic analysis of an immunodominant peptide (B6dom1) encoded by the classical H7 minor histocompatibility locus.
abstract: Of the many minor histocompatibility (H) Ags that have been detected in mice, the ability to induce graft vs host disease (GVHD) after bone marrow transplantation is restricted to a limited number of immunodominant Ags. One such murine Ag, B6dom1, is presented by the H2-Db MHC class I molecule. We present biochemical evidence that the natural B6dom1 peptide is indistinguishable from AAPDNRETF, and we show that this peptide can be isolated from a wide array of tissues, with highest levels from the lymphoid organs and lung. Moreover, we employ a novel, somatic cell selection technique involving CTL-mediated immunoselection coupled with classical genetics, to show that B6dom1 is encoded by the H7 minor H locus originally discovered approximately 40 years ago. These studies provide a molecular genetic framework for understanding B6dom1, and exemplify the fact that mouse minor H loci that encode immunodominant CTL epitopes can correspond to classical H loci originally identified by their ability to confer strong resistance to tumor transplantation. Additionally, these studies demonstrate the utility of somatic cell selection approaches toward resolving H Ag immunogenetics.
authors: The Jackson Laboratory, Bar Harbor, ME 04609, USA. pae@jax.org
authors: P A
authors: PA
authors: Eden
authors: G J
authors: GJ
authors: Christianson
authors: P
authors: P
authors: Fontaine
authors: P J
authors: PJ
authors: Wettstein
authors: C
authors: C
authors: Perreault
authors: D C
authors: DC
authors: Roopenian
mesh_terms: D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002999:Clone Cells; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic / Q000235:genetics; D005260:Female; D016056:Immunodominant Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D015778:Minor Histocompatibility Antigens / Q000235:genetics*; D008912:Minor Histocompatibility Loci / Q000235:genetics* / Q000276:immunology; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics / Q000276:immunology*; D009928:Organ Specificity / Q000276:immunology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology
chemical_list: D016056:Immunodominant Epitopes; D015778:Minor Histocompatibility Antigens; D009842:Oligopeptides