Polymer-bound N-hydroxysuccinimide esters: a column-free fluorescent-labeling method.
Polymer-bound N-hydroxysuccinimide esters of 1-pyrenebutyric acid, 6-carboxyfluorescein diacetate, and biotin were efficiently prepared. Column-free fluorescent- and biotin-labeling reactions of various amines using these resins were successfully demonstrated.
Katoh
M
M
Sagami Chemical Research Center, Sagamihara, Kanagawa, Japan.
Sodeoka
M
M
D000215:Acylation; D001710:Biotin / Q000737:chemistry; D019921:Biotinylation / Q000379:methods; D004952:Esters / Q000138:chemical synthesis*; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000378:metabolism; D008956:Models, Chemical; D015336:Molecular Probe Techniques; D011721:Pyrenes / Q000737:chemistry; D013388:Succinimides / Q000138:chemical synthesis*
D004952:Esters; D005452:Fluoresceins; D005456:Fluorescent Dyes; D011721:Pyrenes; D013388:Succinimides; C093616:6-carboxyfluorescein diacetate; C002218:1-pyrenebutyrate; D001710:Biotin