Large-scale transient expression in mammalian cells for recombinant protein production.
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.
Wurm
F
F
Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
Bernard
A
A
D000256:Adenoviridae / Q000235:genetics; D000524:Alphavirus / Q000235:genetics; D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D002522:Chlorocebus aethiops; D003001:Cloning, Molecular; D006224:Cricetinae; D005822:Genetic Vectors; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D014616:Vaccinia virus / Q000235:genetics
D011994:Recombinant Proteins