Biallelic polymorphism in the intron region of beta-tubulin gene of Cryptosporidium parasites.
Nucleotide sequencing of polymerase chain reaction amplified intron region of the Cryptosporidium parvum beta-tubulin gene in 26 human and 15 animal isolates revealed distinct genetic polymorphism between the human and bovine genotypes. The separation of 2 genotypes of C. parvum is in agreement with our previous genotyping data based on the thrombospondin-related adhesion protein (TRAP-C2) gene, indicating these genotype characteristics are linked at 2 genetic loci. Characterization of Cryptosporidium muris and Cryptosporidium serpentis has further shown that non-parvum Cryptosporidium parasites have beta-tubulin intron sequences identical to bovine genotype of C. parvum. Thus, results of this study confirm the lineage of 2 genotypes of C. parvum at 2 genetic loci and suggest a need for extensive characterization of various Cryptosporidium spp.
Sulaiman
I M
IM
Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3717, USA.
Lal
A A
AA
Arrowood
M J
MJ
Xiao
L
L
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003458:Cryptosporidium / Q000235:genetics*; D016785:Cryptosporidium parvum / Q000235:genetics*; D017931:DNA Primers / Q000737:chemistry; D016054:DNA, Protozoan / Q000737:chemistry; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007438:Introns / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D014404:Tubulin / Q000235:genetics*
D017931:DNA Primers; D016054:DNA, Protozoan; D014404:Tubulin