title: Morphology of peri-implant regenerated bone, in sheep's tibia, by means of guided tissue regeneration.
abstract: BACKGROUND
  No data on the resistance to functional loads of bone regenerated by means of guided tissue regeneration, can be found. In this paper the new-formed bone is analyzed, in cases that see the surgical creation of defects in testing animals. The morphological aspects of perimplant bone, regenerated by means of bone substitutes or not, were evaluated.
  
  
  METHODS
  A coronal defect was created by means of a counter-sink bur. The following step provided for osseointegrated fixtures, with a machined or blasted (TiOblast) surface, to be installed. Some bone defects were filled with Bio-Oss, and covered with resorbable membrane (Vycril), others were left unfilled. The animals were sacrificed after different periods of time (24 and 45 days). Some thick sections (200 microns) underwent then microradiography and were examined with a microscope, under transmitted and polarized light. Other sections, thin (5 microns), were coloured with toluidine blue, Gomori's Stain and Solochrome Cyanine/Congo red.
  
  
  RESULTS
  Data confirm the excellent behaviour of bovine bone and of TiO blasted fixtures. The experimental results bring into evidence that, in cases of Guided Bone Regeneration (GBR) by means of membrane, the use of fixtures with a blasted surface (TiOblast), and of Bio-Oss as bone substitute, made osseointegration easier.
  
  
  CONCLUSIONS
  Results, moreover, suggest that in case of implant surgery with GBR, in order to allow the maturation of regenerated bone, it's healing time must be prolonged.
authors: Department of Morphological Sciences, School of Dentistry, University of Pavia.
authors: R
authors: R
authors: Rodriguez y Baena
authors: D
authors: D
authors: Zaffe
authors: U E
authors: UE
authors: Pazzaglia
authors: S
authors: S
authors: Rizzo
mesh_terms: D000818:Animals; D003757:Dental Implantation; D006801:Humans; D012756:Sheep; D014071:Tooth Abnormalities / Q000601:surgery*