Establishment of a human hemangiosarcoma cell line (ISO-HAS).
A cell line (ISO-HAS) has been established from tumor tissue of a human hemangiosarcoma arising on the scalp by the use of conditioned medium from a murine-phenotypic angiosarcoma cell line (ISOS-1). Cells have been cultured for more than 2 years with up to 100 passages. The cells retained endothelial-cell properties, such as a characteristic cobblestone appearance at confluency, contact-inhibited growth, active uptake of acetylated low-density lipoprotein labeled with 1,1-dioctadecyl 1,3,3,3,3-tetramethyl-indocarbocyanine perchlorate (DiI-Ac-LDL) and CD31 expression. However, they were weakly positive for von-Willebrand-factor (vWf) antigen and for binding of Ulex europaeus agglutinin-I (UEA-I) lectin, and lacked tube-formation activity. These findings indicate that ISO-HAS is a poorly differentiated endothelial cell line. ISO-HAS cells showed accumulation of p53 protein in the nuclei, and a new-typed p53-gene point mutation was found in exon 7 at codon 240. When inoculated s.c. into severe-combined-immunodeficiency (SCID) mice, the cells showed solid-tumor growth that caused death. These properties suggest that ISO-HAS is a malignant endothelial cell line with high tumorigenicity.
Masuzawa
M
M
Department of Dermatology, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan. mikio@med.kitasato-u.ac.jp
Fujimura
T
T
Hamada
Y
Y
Fujita
Y
Y
Hara
H
H
Nishiyama
S
S
Katsuoka
K
K
Tamauchi
H
H
Sakurai
Y
Y
D000818:Animals; D002455:Cell Division / Q000502:physiology; D017077:Culture Media, Conditioned; D004730:Endothelium, Vascular / Q000473:pathology*; D006394:Hemangiosarcoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D010641:Phenotype; D014407:Tumor Cells, Cultured
D017077:Culture Media, Conditioned