title: Innate resistance to experimental Trypanosoma congolense infection: differences in IL-10 synthesis by macrophage cell lines from resistant and susceptible inbred mice.
abstract: BALB/c and C57Bl/6 mice differ in resistance to T. congolense infections. We investigated the production of various cytokines (IL-10, IL-6, TNF-alpha and TGF-beta) by macrophages from these mice. Macrophage cell lines (BALB.BM cells) of BALB/c mice but not (ANA-I cells)of C57BL/6 mice constitutively produced IL-10. Challenge of these cells with trypanosomes induced the production of 50-100 times more IL-10 in BALB.BM cells than in ANA-1 cells. Pre-incubation of the cell lines with IFN-gamma. prior to the trypanosome challenge, further upregulated this IL-10 production in BALB.BM but not in ANA-1 cells. Primary cultures of bone marrow-derived macrophages (BMDM) from BALB/c mice also produced more IL-10 following challenge with IFN-gamma and opsonized trypanosomes than did the C57Bl/6 BMDM. Similarly after challenge with trypanosomes, BALB.BM and BALB/c BMDM produced significantly more IL-6 than did the analogous cells from the C57Bl/6 mice following such challenges. Higher steady state levels of TNF-alpha mRNA accumulated in ANA-1 cells than in BALB.BM cells following challenge with IFN-gamma and opsonized trypanosomes. Findings of this study for the first time indicate a differential regulation of cytokines (IL-10, IL-6 and TNF-alpha) in macrophages of mice that significantly differ in their susceptibility to infections with T. congolense.
authors: Department of Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada.
authors: R S
authors: RS
authors: Kaushik
authors: J E
authors: JE
authors: Uzonna
authors: D
authors: D
authors: Radzioch
authors: J R
authors: JR
authors: Gordon
authors: H
authors: H
authors: Tabel
mesh_terms: D000818:Animals; D002460:Cell Line; D004198:Disease Susceptibility; D015870:Gene Expression; D007113:Immunity, Innate / Q000276:immunology; D016898:Interferon-alpha / Q000235:genetics; D016753:Interleukin-10 / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta / Q000235:genetics; D014348:Trypanosoma congolense / Q000276:immunology*
chemical_list: D016898:Interferon-alpha; D012333:RNA, Messenger; D016212:Transforming Growth Factor beta; D016753:Interleukin-10