High frequency of persistent hyperplastic primary vitreous and cataracts in p53-deficient mice.
In order to investigate whether the p53 gene product plays a role in normal eye development, age matched p53-deficient mice and wild-type controls were sacrificed from day 2 to day 21 after birth. Eyes were paraffin-embedded and sectioned. Serial sections were taken at the level of the tunica vasculosa lentis and the hyaloid artery. The terminal dUTP nick-end labelling technique (TUNEL) was used to detect the number of cells displaying DNA fragmentation within these structures. Eyes were also prepared for scanning electron microscopy and resin embedded for semi-thin sections. Adult wild-type mice and p53-deficient mice were examined ophthalmoscopically in vivo. Ophthalmoscopical examination of mice completely deficient in p53 revealed them to be normal except for the persistence of the hyaloid vasculature, a structure that normally regresses during eye development. In adult animals there was also a high frequency of cataracts. Using morphological assessment and TUNEL we could show that in normal mice, regression of the primary vitreous, which includes the hyaloid artery, the vasa hyaloidea propria as well as the tunica vasculosa lentis, occurs via apoptotic cell death within 5 - 6 weeks after birth. The number of TUNEL-positive cells within these structures was significantly reduced in the p53-deficient mice in which parts of the hyaloid vasculature persisted and developed into a fibro-vascular retrolental plaque analogous to persistent hyperplastic primary vitreous (PHPV) described in humans. As in humans, PHPV in mice resulted in the development of cataracts. We have identified a role for p53-dependent apoptosis in the regression of the hyaloid vasculature and tunica vasculosa lentis. Our results provide further evidence for the importance of p53 in normal development and provide the first detailed evidence of its role in postnatal development in remodelling the developing eye.
Reichel
M B
MB
Department of Molecular Genetics, Institute of Ophthalmology, University College London, Bath Street, London EC1V 9EL, UK.
Ali
R R
RR
D'Esposito
F
F
Clarke
A R
AR
Luthert
P J
PJ
Bhattacharya
S S
SS
Hunt
D M
DM
D000367:Age Factors; D000818:Animals; D001082:Aqueous Humor; D002386:Cataract / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D005123:Eye / Q000254:growth & development / Q000473:pathology / Q000648:ultrastructure; D005124:Eye Abnormalities / Q000235:genetics* / Q000473:pathology* / Q000503:physiopathology; D015972:Gene Expression Regulation, Neoplastic; D006965:Hyperplasia; D020287:In Situ Nick-End Labeling; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008855:Microscopy, Electron, Scanning; D009887:Ophthalmoscopy; D012161:Retinal Artery / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*; D014822:Vitreous Body / Q000098:blood supply / Q000473:pathology / Q000503:physiopathology
D016159:Tumor Suppressor Protein p53