Cytolethal distending toxin genes in Campylobacter jejuni and Campylobacter coli isolates: detection and analysis by PCR.
Campylobacter jejuni produces a toxin called cytolethal distending toxin (CDT). Knowledge of the prevalence and homogeneity of Campylobacter sp. cdt genes is incomplete. In this work, we identified four PCR primer pairs that collectively amplified cdt genes in all of the C. jejuni and Campylobacter coli strains tested. Restriction analyses of the cdt PCR products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the same species. Consequently, it may be possible to speciate C. jejuni and C. coli isolates on the basis of restriction patterns within their cdt genes.
Eyigor
A
A
Department of Animal Sciences, College of Agriculture, University of Kentucky, Lexington, Kentucky 40546-0215, USA.
Dawson
K A
KA
Langlois
B E
BE
Pickett
C L
CL
D001427:Bacterial Toxins / Q000235:genetics* / Q000633:toxicity; D017000:Campylobacter coli / Q000235:genetics / Q000472:pathogenicity*; D016123:Campylobacter jejuni / Q000235:genetics / Q000472:pathogenicity*; D005798:Genes, Bacterial; D006367:HeLa Cells; D006801:Humans; D016133:Polymerase Chain Reaction
D001427:Bacterial Toxins; C067044:cytolethal distending toxin