Distribution and characterization of immunoreactive prolactin-releasing peptide (PrRP) in rat tissue and plasma.
We established a sensitive and specific two-site enzyme immunoassay (EIA) for prolactin-releasing peptide (PrRP) using two region-specific monoclonal antibodies. We investigated the tissue distribution and the plasma concentration of immunoreactive (ir-) PrRP in rats using this assay. Ir-PrRP was widely distributed in the central nervous system and pituitary gland. The highest concentration of ir-PrRP was found in the hypothalamus. In peripheral tissues, appreciable levels of ir-PrRP were found only in the adrenal gland. The mean plasma concentration of ir-PrRP was 0.13 +/- 0.01 fmol/ml (mean +/- SEM). In reverse-phase and gel-filtration high performance liquid chromatography, hypothalamic ir-PrRP eluted at a position identical to that of PrRP31 and PrRP20. On the other hand, ir-PrRP from the adrenal gland and plasma eluted only at the position of synthetic PrRP31, indicating that molecular forms of ir-PrRP in vivo differed among tissues.
Matsumoto
H
H
Pharmaceutical Discovery Division, Takeda Chemical Industries Ltd., Wadai-10, Tsukuba Ibaraki, 300-4293, Japan. Matsumoto_Hirokazu@takeda.co.jp
Murakami
Y
Y
Horikoshi
Y
Y
Noguchi
J
J
Habata
Y
Y
Kitada
C
C
Hinuma
S
S
Onda
H
H
Fujino
M
M
D000311:Adrenal Glands / Q000378:metabolism; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D002490:Central Nervous System / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D007028:Hypothalamic Hormones / Q000097:blood* / Q000276:immunology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D008297:Male; D009479:Neuropeptides / Q000097:blood* / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000097:blood / Q000276:immunology / Q000378:metabolism; D010902:Pituitary Gland / Q000378:metabolism; D056690:Prolactin-Releasing Hormone; D020033:Protein Isoforms / Q000097:blood / Q000276:immunology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007028:Hypothalamic Hormones; D009479:Neuropeptides; D010446:Peptide Fragments; C534379:Prlh protein, rat; D056690:Prolactin-Releasing Hormone; D020033:Protein Isoforms