Cloning and characterization of metallothionein cDNAs in the mussel Mytilus edulis L. digestive gland.
Metallothioneins are small metal-binding proteins found in all species of animals and are transcriptionally-induced by heavy metal ions, oxidative stresses, and inflammation. In the blue sea mussel, Mytilus edulis, several apparent subtypes of each isoform have been purified and biochemically sequenced. To determine whether the high number of metallothionein forms present in M. edulis were specific to the digestive gland, and to understand how these proteins evolved, we cloned five variants of metallothionein from M. edulis. MT10 and MT20 isoform fragments were amplified by PCR, and used as radiolabelled probes to screen digestive gland cDNA libraries. The MT10 transcripts were 321-353 nucleotides long and the MT20 transcripts, 513-555 nucleotides. Previously identified primary structures of MT10 subtypes were confirmed and, in addition, a novel subtype was identified. Expression of MT10 and MT20 isoforms shown by clonal representation and Northern blot analysis indicated that the MT10 message was more prevalent than the MT20 message. Only the MT20 II transcript could be identified among the MT20 clones. The high degree of untranslated region similarity between each isoform indicates that these additional forms are recent gene duplication events in the Mytilus lineage. Exposure of 0.4 mg l-1 of cadmium to the mussels resulted in a marked increase in both mRNAs suggesting that the MT20 isoform represents a primarily inducible metallothionein not highly expressed under basal conditions.
Barsyte
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Institute of Ecology, Akademijos 2, Vilnius, Lithuania. momlfdb2@fs2.scg.man.ac.uk
White
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Lovejoy
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D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics*; D004064:Digestive System / Q000737:chemistry / Q000378:metabolism; D015723:Gene Library; D008668:Metallothionein / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism
D018076:DNA, Complementary; D012333:RNA, Messenger; D008668:Metallothionein