title: An improved system for selection of forward mutations in an Escherichia coli supF gene carried by plasmids.
abstract: An improved system to examine forward mutations that occurred in the supF gene of Escherichia coli carried on a multicopy plasmid is described. The system was validated by measuring spontaneous mutations of supF plasmids propagated in wild-type, recA- and mutM- mutY- E. coli strains, the mutation frequencies of which were 1.3 x 10(-7), 6.3 x 10(-7) and 1.5 x 10(-6), respectively. Sequence analysis of the supF mutant plasmids revealed that G:C-->T:A and G:C-->C:G transversions dominated. This improved system allows rapid scoring and sequencing forward mutations in the supF gene, thus permitting its use as a genetic target for repair and mutagenesis studies in bacteria and mammalian cells.
authors: Biological Institute, Graduate School of Science, Tohoku University, Sendai, Japan.
authors: F
authors: F
authors: Obata
authors: T
authors: T
authors: Nunoshiba
authors: T
authors: T
authors: Hashimoto-Gotoh
authors: K
authors: K
authors: Yamamoto
mesh_terms: D004926:Escherichia coli / Q000235:genetics*; D016153:Genes, Suppressor; D009154:Mutation; D010957:Plasmids; D012343:RNA, Transfer
chemical_list: C096275:supF tRNA; D012343:RNA, Transfer