title: Increased intracellular calpain detection in experimental focal cerebral ischemia.
abstract: Calpains are intracellular proteinases whose proteolytic activity is directed mainly against the cytoskeleton and regulatory proteins. We studied the presence of calpain by immunohistochemistry in a rat model of reversible focal cerebral ischemia (3 h) at various times of reperfusion. The numbers of calpain-positive cells on the ischemic side were compared with the non-ischemic side. In controls only 2 +/- 1% cells were positive, whereas the cortex of the ischemic vs the non-ischemic side showed 88 +/- 3% vs 13 +/- 4% calpain-positive cells (p < 0.001), and the basal ganglia 47 +/- 3% vs 13 +/- 4% (p < 0.01) after 3 h ischemia and 24 h reperfusion. This is the first demonstration of elevated intracellular levels of calpains in areas of cerebral ischemia. Longer reperfusion resulted in an increase in calpain positivity.
authors: Department of Neurology, Klinikum Grosshadern, Munich, Germany.
authors: M
authors: M
authors: Liebetrau
authors: B
authors: B
authors: Staufer
authors: E A
authors: EA
authors: Auerswald
authors: D
authors: D
authors: Gabrijelcic-Geiger
authors: H
authors: H
authors: Fritz
authors: C
authors: C
authors: Zimmermann
authors: T
authors: T
authors: Pfefferkorn
authors: G F
authors: GF
authors: Hamann
mesh_terms: D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism / Q000473:pathology; D002545:Brain Ischemia / Q000378:metabolism* / Q000473:pathology; D002154:Calpain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D007424:Intracellular Fluid / Q000378:metabolism*; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D051381:Rats; D012016:Reference Values; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D013997:Time Factors
chemical_list: D002154:Calpain