Vaccination of pigs against pseudorabies virus with plasmid DNA encoding glycoprotein D.
We analysed the ability of a plasmid carrying the gene encoding glycoprotein D (gD) of pseudorabies virus (PRV) to induce humoral and cell-mediated immune responses and assessed the protection provided by PRV-gD DNA vaccination against challenge infection with PRV. Immunization with plasmid PRV-gD induced neutralizing antibodies and lymphocyte proliferative responses both in mice and pigs. Moreover, when challenged with virulent PRV six weeks following the last immunization, PRV-gD DNA vaccinated pigs excreted virus for a significantly shorter period and showed less clinical symptoms than pigs vaccinated with a control plasmid. Thus, in the target animal, DNA vaccination with PRV-gD DNA induces protective immunity against challenge infection.
Haagmans
B L
BL
Department of Infectious Diseases and Immunology, Veterinary Faculty, Utrecht University, Netherlands. b.haagmans@vetmic.dgk.ruu.nl
van Rooij
E M
EM
Dubelaar
M
M
Kimman
T G
TG
Horzinek
M C
MC
Schijns
V E
VE
Bianchi
A T
AT
D000818:Animals; D003001:Cloning, Molecular; D005260:Female; D011558:Herpesvirus 1, Suid / Q000235:genetics / Q000276:immunology*; D007739:L Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010957:Plasmids; D011557:Pseudorabies / Q000517:prevention & control*; D013552:Swine; D014611:Vaccination / Q000662:veterinary*; D019444:Vaccines, DNA / Q000276:immunology; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000276:immunology
D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; C107752:glycoprotein D, pseudorabies virus