title: Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia.
abstract: Recent identification of two receptors for the adenovirus fiber protein, coxsackie B and adenovirus type 2 and 5 receptor (CAR), and the major histocompatibility complex (MHC) Class I alpha-2 domain allows the molecular basis of adenoviral infection to be investigated. Earlier work has shown that human airway epithelia are resistant to infection by adenovirus. Therefore, we examined the expression and localization of CAR and MHC Class I in an in vitro model of well differentiated, ciliated human airway epithelia. We found that airway epithelia express CAR and MHC Class I. However, neither receptor was present in the apical membrane; instead, both were polarized to the basolateral membrane. These findings explain the relative resistance to adenovirus infection from the apical surface. In contrast, when the virus was applied to the basolateral surface, gene transfer was much more efficient because of an interaction of adenovirus fiber with its receptors. In addition, when the integrity of the tight junctions was transiently disrupted, apically applied adenovirus gained access to the basolateral surface and enhanced gene transfer. These data suggest that the receptors required for efficient infection are not available on the apical surface, and interventions that allow access to the basolateral space where fiber receptors are located increase gene transfer efficiency.
authors: Departments of Internal Medicine, Howard Hughes Medical Institute, Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, Iowa 52242, USA.
authors: R W
authors: RW
authors: Walters
authors: T
authors: T
authors: Grunst
authors: J M
authors: JM
authors: Bergelson
authors: R W
authors: RW
authors: Finberg
authors: M J
authors: MJ
authors: Welsh
authors: J
authors: J
authors: Zabner
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D001485:Basement Membrane / Q000378:metabolism; D001980:Bronchi / Q000737:chemistry* / Q000821:virology*; D016764:Cell Polarity; D002478:Cells, Cultured; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D004770:Enterovirus / Q000378:metabolism*; D004847:Epithelial Cells / Q000737:chemistry / Q000821:virology; D018014:Gene Transfer Techniques; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D011991:Receptors, Virus / Q000378:metabolism*; D013499:Surface Properties; D019108:Tight Junctions / Q000378:metabolism; D014132:Trachea / Q000737:chemistry* / Q000821:virology*
chemical_list: C505032:CFTR protein, human; C568771:CLMP protein, human; D062727:Coxsackie and Adenovirus Receptor-Like Membrane Protein; D015395:Histocompatibility Antigens Class I; D011991:Receptors, Virus; D019005:Cystic Fibrosis Transmembrane Conductance Regulator