title: Surface phenotype analysis of CD16+ monocytes from leukapheresis collections for peripheral blood progenitors.
abstract: In peripheral blood progenitor cell (PBPC) collections from patients with solid tumour or haematological malignancy, monocytes were separated into two subpopulations. The majority of monocytes expressed CD14 at a high density without CD16 antigen (the CD14+CD16- monocytes). The remaining monocytes co-expressed CD14 and CD16 (the CD14+CD16+ monocytes). These CD14+CD16+ monocytes amounted to 20.6 +/- 15.8%, while those in peripheral blood (PB) obtained from healthy volunteers were 7.3 +/- 3.1% (P < 0.05). When subdividing the CD14+CD16+ monocytes into CD14brightCD16dim and CD14dimCD16bright cells, both populations were found to be increased in PBPC collections. Since typical CD14+CD16+ monocytes are the CD14dimCD16bright population, we compared the additional surface antigens on CD14dimCD16bright monocytes with those of CD14+CD16- monocytes. In PBPC collections, the CD14dimCD16bright monocytes exhibited lower levels of CD11b, CD15, CD33 and CD38 expression and higher levels of CD4, CD11a, CD11c and MHC class II, and also revealed a higher percentage of CD4+ cells and a lower percentage of CD15+ cells and CD38+ cells, compared with the CD14+CD16- monocytes. When compared with the CD14dimCD16bright monocytes in PB, those in PBPC collections exhibited higher expression of CD4 and lower expression of CD11b, and also showed higher percentages of CD4+ cells and CD38+ cells and a lower percentage of CD11b+ cells. These results suggest that PBPC collections may be rich in the CD14+CD16+ monocytes in which the proportion of the immature population is increased. It is likely that these monocytes participate in the haematological and immune recovery after PBPC transplantation.
authors: First Department of Internal Medicine, Kurume University School of Medicine, Japan. mtanaka@med.kurume-u.ac.jp
authors: M
authors: M
authors: Tanaka
authors: J
authors: J
authors: Honda
authors: Y
authors: Y
authors: Imamura
authors: K
authors: K
authors: Shiraishi
authors: K
authors: K
authors: Tanaka
authors: K
authors: K
authors: Oizumi
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000302:isolation & purification*; D001943:Breast Neoplasms / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007937:Leukapheresis; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000302:isolation & purification; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology; D017452:Receptors, IgG / Q000302:isolation & purification
chemical_list: D015703:Antigens, CD; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG