title: Enhanced capacity of a widespread strain of Mycobacterium tuberculosis to grow in human macrophages.
abstract: To determine whether the extent of spread of Mycobacterium tuberculosis strains in the community correlated with their capacity to replicate in human macrophages, intracellular growth rates of M. tuberculosis patient isolates were measured. Strain 210 caused disease in 43 patients in central Los Angeles, 3 "small-cluster" strains caused disease in 8-23 patients, and 5 "unique" strains each caused disease in only 1 patient who was positive by sputum acid-fast smear and spent substantial amounts of time at homeless shelters that were tuberculosis transmission sites. Strain 210 isolates grew significantly more rapidly than small-cluster and unique strains in macrophages. All strains elicited production of similar amounts of tumor necrosis factor-alpha, interleukin (IL)-6, IL-10, and IL-12 and were equally susceptible to reactive nitrogen intermediates. It was concluded that the extensive spread of an M. tuberculosis strain correlated with its capacity to replicate rapidly in human macrophages, which may be a marker of virulence.
authors: Center for Pulmonary and Infectious Disease Control, University of Texas Health Center at Tyler, Tyler, TX 75710, USA. mingzhang@coh. edu
authors: M
authors: M
authors: Zhang
authors: J
authors: J
authors: Gong
authors: Z
authors: Z
authors: Yang
authors: B
authors: B
authors: Samten
authors: M D
authors: MD
authors: Cave
authors: P F
authors: PF
authors: Barnes
mesh_terms: D002478:Cells, Cultured; D015169:Colony Count, Microbial; D006801:Humans; D007378:Interleukins / Q000096:biosynthesis; D008264:Macrophages / Q000276:immunology / Q000382:microbiology*; D009000:Monocytes / Q000166:cytology / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification / Q000472:pathogenicity; D009573:Nitrites / Q000494:pharmacology; D014376:Tuberculosis / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D014774:Virulence
chemical_list: D007378:Interleukins; D009573:Nitrites; D014409:Tumor Necrosis Factor-alpha