title: Evidence suggesting that the angiotensin II-sensitive intracellular Ca2+ pool is reloaded from the external space in adrenal glomerulosa cells.
abstract: Adrenal glomerulosa cells prelabeled with 45Ca2+ and perifused for 10 min with 10 nM angiotensin II (AII) in a dynamic perifusion system show a biphasic response with an initial transient increase in 45Ca2+ efflux, followed by a sustained phase of increased 45Ca2+ efflux. When labeled adrenal golmerulosa cells were treated with 10 nM AII for three consecutive periods of 5 min, the transient increase in 45Ca2+ efflux was observed only in the first period. However, when 40Ca2+ was measured in the perifusate using a Ca2+-sensitive electrode coupled to the perifusion system, a transient increase in 40Ca2+ efflux was observed in each period of AII treatment. Exposing the cells to AII for 1 min, the amount of 40Ca2+ effluxed out of the cells was 58.3 +/- 8.4 nmol/10(8) cells. In contrast, when the cells were exposed to an increase in the external potassium (K+) concentration of 4 to 12 mM during 1 min of perifusion, the amount of 40Ca2+ effluxed was 16 +/- 5 nmol/10(8) cells. These results indicate that AII induces an increase in the Ca2+ concentration in a local domain outside of the plasma membrane. This Ca2+ comes from AII-induced intracellular Ca2+ depletion and may play a role in refilling intracellular Ca2+ stores.
authors: Department of Physiology and Biophysics, Faculty of Medicine, University of Chile, Santiago.
authors: R H
authors: RH
authors: Foster
authors: A M
authors: AM
authors: Rojas
mesh_terms: D000450:Aldosterone / Q000096:biosynthesis; D000804:Angiotensin II / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D002478:Cells, Cultured; D003620:Dantrolene / Q000494:pharmacology; D066298:In Vitro Techniques; D009125:Muscle Relaxants, Central / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D013997:Time Factors; D015384:Zona Glomerulosa / Q000378:metabolism* / Q000473:pathology
chemical_list: D009125:Muscle Relaxants, Central; D000804:Angiotensin II; D000450:Aldosterone; D003620:Dantrolene; D011188:Potassium; D002118:Calcium