Assay of tinidazole in human serum by high-performance thin-layer chromatography--comparison with high-performance liquid chromatography.
Determination of tinidazole in human serum by high-performance thin-layer chromatography (HPTLC) is presented. It includes use of 10 x 10 cm plates coated with silica gel 60 and chloroform-acetonitrile-acetic acid (60 + 40 + 2) as mobile phase. Quantitation was performed by densitometry at 320 nm. The linearity (1-10 ng), precision (6%), reproducibility (5%), recovery (96%), and detection limit (1 mg/L) of tinidazole determination by HPTLC were comparable with corresponding method parameters by reversed-phase HPLC. A satisfactory correlation was found between the 2 analytical methods. The procedure was used to quantitate tinidazole in patient sera.
Guermouche
M H
MH
Université des Sciences et de la Technologie Houari Boumedienne, Institut de Chimie, Alger, Algeria.
Habel
D
D
Guermouche
S
S
D000994:Antitrichomonal Agents / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014011:Tinidazole / Q000097:blood*
D000994:Antitrichomonal Agents; D014011:Tinidazole