Long-term changes in tyrosine phosphorylation of the abundant nuclear proteins during granulocytic differentiation of HL-60 cells.
The two-dimensional electrophoretic patterns of nuclear proteins and their tyrosine phosphorylation were compared for HL-60 cells before and after differentiation induction to granulocytes by dimethyl sulfoxide, all-trans retinoic acid and N6,O2-dibutyryl adenosine 3':5'-cyclic monophosphate. Regardless of the inducer used, some nuclear proteins, which are tyrosine-phosphorylated in proliferating HL-60 cells, undergo gradual dephosphorylation 12-72 h after induction of differentiation, followed by drastic dephosphorylation during maturation to granulocytes. At least 13 nuclear proteins with a molecular mass of 35-110 kDa are dephosphorylated, and 6 nuclear proteins undergo tyrosine phosphorylation. Analysis of the nuclear proteins differentially extracted by salt and detergents indicates that changes in their tyrosine phosphorylation during the maturation stage of differentiating granulocytes occur mainly in proteins which are abundant in nucleoplasm, chromatin and residual nuclear structures. The abundance of these proteins, residing in the nuclear structures, and their long-term modification in phosphorylation during the maturation stages of differentiation strongly suggest that tyrosine phosphorylation of these proteins is involved in reorganization of the differentiating cell nucleus.
Gineitis
A
A
Laboratory of Developmental Biology, Institute of Biochemistry, Vilnius, Lithuania.
Treigyte
G
G
Savickiene
J
J
Shanbhag
V P
VP
Stigbrand
T
T
D003994:Bucladesine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018922:HL-60 Cells; D006801:Humans; D019891:Leukopoiesis; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology; D014443:Tyrosine / Q000378:metabolism*
D009687:Nuclear Proteins; D014443:Tyrosine; D014212:Tretinoin; D003994:Bucladesine; D004121:Dimethyl Sulfoxide