A 1.5-Mb contig within the cat eye syndrome critical region at human chromosome 22q11.2.
We have constructed a 1.5-Mb contig spanning the distal half of the critical region for cat eye syndrome on human chromosome 22 from D22S543 to D22S181. The contig consists of 20 P1 artificial chromosome (PAC) clones and 11 bacterial artificial chromosome (BAC) clones screened from 2 BAC and 2 PAC libraries. Continuous overlap between the clones was confirmed using vectorette PCR and riboprobes. Despite the instability of this region in a previous YAC contig, only 1 BAC showed a minor instability and then in only one isolation. This contig is now providing the basis for genomic sequencing and gene identification in the cat eye syndrome critical region.
Johnson
A
A
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada.
Minoshima
S
S
Asakawa
S
S
Shimizu
N
N
Shizuya
H
H
Roe
B A
BA
McDermid
H E
HE
D000015:Abnormalities, Multiple / Q000235:genetics*; D001419:Bacteria / Q000235:genetics; D017101:Bacteriophage P1 / Q000235:genetics; D002892:Chromosomes, Human, Pair 22 / Q000235:genetics*; D003103:Coloboma / Q000235:genetics*; D020451:Contig Mapping; D004247:DNA / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005819:Genetic Markers; D006801:Humans; D015183:Restriction Mapping; D013577:Syndrome
D005819:Genetic Markers; D004247:DNA