title: fcsA29 mutation is an allele of polA gene of Escherichia coli.
abstract: The cold-sensitive fcsA29 mutation of Escherichia coli was found to be a new type of cold-sensitive allele of the polA gene encoding DNA polymerase I, caused by an Asp(116)-->Asn change in the 5'-->3' exonuclease domain. The fcsA29 mutant showed typical polA mutant phenotypes such as UV sensitivity and unacceptability of recA mutation. Cold-sensitive growth of the mutant was suppressed by introduction of a sulA mutation, indicating that cell filamentation was due to the SOS response.
authors: Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
authors: K
authors: K
authors: Nagano
authors: M
authors: M
authors: Wachi
authors: A
authors: A
authors: Takada
authors: F
authors: F
authors: Takaku
authors: T
authors: T
authors: Hirasawa
authors: K
authors: K
authors: Nagai
mesh_terms: D000483:Alleles; D002470:Cell Survival / Q000528:radiation effects; D003080:Cold Temperature; D004256:DNA Polymerase I / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics* / Q000254:growth & development; D005816:Genetic Complementation Test; D017354:Point Mutation; D011938:Rec A Recombinases / Q000235:genetics; D013014:SOS Response, Genetics / Q000235:genetics; D014466:Ultraviolet Rays
chemical_list: D011938:Rec A Recombinases; D004256:DNA Polymerase I