Studies on the determination of diacetyl guanfubase A concentration in rabbit plasma and pharmacokinetics.
A gas chromatographic method for monitoring diacetyl guanfubase A in plasma is described. The procedure involved a single solvent extraction of drug from rabbit plasma into ethyl acetate with guanfubase A as an internal standard. The extract was analyzed subsequently on a gas chromatograph equipped with a hydrogen flame ionization detector. The recovery was 86.43% +/- 6.90% (+/- SD); the RSD of within-day and between-day was 2.81%-5.26% and 5.22%-8.24%, respectively; the regression line was linear over the concentration range of 25-200 micrograms/mL, the limit of detection was 10 micrograms/mL. No endogeneous interference was found in chromatograms of the biological samples. This method was applied to the pharmacokinetic study of diacetyl guanfubase A in rabbits.
Wang
W H
WH
College of Pharmacy, Shandong Medical University, Jinan, People's Republic of China.
Wang
R B
RB
Zhang
J R
JR
Liu
C H
CH
Pan
S J
SJ
D000470:Alkaloids / Q000097:blood* / Q000493:pharmacokinetics; D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000097:blood* / Q000493:pharmacokinetics; D002849:Chromatography, Gas; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results
D000470:Alkaloids; D000889:Anti-Arrhythmia Agents; C119184:diacetyl guan-fu base A