[Detection of the hog cholera virus using the polymerase chain reaction].
A rapid and highly sensitive method for detecting hog cholera virus (HCV) based on a reverse transcription of the polymerase chain reaction (RT-PCR) is developed. Primers complementary to the most homologous sites of virus genome in an area coding the precursor for glycoproteins gp44/gp48 are selected. Detection of the virus in pathological material by the RT-PCR showed that use of these primers in amplification allows detection of different HCV strains.
Semenikhin
V I
VI
Puzyrev
A T
AT
Oreshkova
S F
SF
Donchenko
A S
AS
Chekishev
V M
VM
Il'ichev
A A
AA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002460:Cell Line; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000302:isolation & purification*; D018076:DNA, Complementary; D004279:DNA, Viral; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D018076:DNA, Complementary; D004279:DNA, Viral