title: Estimates of mouse oviductal fluid tonicity based on osmotic responses of embryos.
abstract: Zygotes and early cleavage-stage embryos are very sensitive to increased osmolality in vitro, although the tonicity of their in vivo environment, oviductal fluid, is unknown. A preference for low osmolality in vitro might imply similar conditions in vivo or be specific to culture. Previous electron probe x-ray microanalysis measurements of total ion content predicted oviductal fluid osmolalities of 310-360 mOs/kg, higher than osmolalities tolerated by mouse zygotes in vitro. However, such indirect estimates may not reflect the tonicity experienced by embryos. We have now used embryos themselves as osmosensors to determine the tonicity of mouse oviductal fluid. In one method, we measured the mean volume of zygotes in undiluted oviductal fluid and compared this to the mean volumes measured for zygotes in media spanning a range of osmolalities. The osmolality corresponding to the measured mean volume in oviductal fluid was taken to be isotonic. In another, independent method, the sizes of zygotes and two-cell embryos were measured as a function of time beginning immediately after removal from oviducts. The osmolality in which the embryos neither swelled nor shrank was taken to be isotonic. Both methods yielded approximately the same range for the tonicity of oviductal fluid: around 290-300 mOs/kg.
authors: Loeb Research Institute and Human IVF Program, Ottawa Hospital, Ottawa, Ontario, Canada K1Y 4E9.
authors: J L
authors: JL
authors: Collins
authors: J M
authors: JM
authors: Baltz
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D003470:Culture Media; D004622:Embryo, Mammalian / Q000737:chemistry / Q000378:metabolism*; D005187:Fallopian Tubes / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D051379:Mice; D009924:Organ Culture Techniques; D009994:Osmolar Concentration; D011247:Pregnancy; D015053:Zygote / Q000378:metabolism
chemical_list: D003470:Culture Media