Isolation of human hepatocytes after hepatic warm and cold ischemia: a practical approach using University of Wisconsin solution.
A method is described for isolating human hepatocytes from tissue fragments after warm and cold ischemia as experienced during hepatic resections. Cells with a high trypan blue dye exclusion and good culture characteristics were isolated by employing an initial tissue perfusion with UW solution. The method could facilitate transfer of liver tissues between distant centers for cell isolation studies.
Caruana
M
M
University Department of Surgery and Liver Transplant Unit, Royal Free and University College Medical School, London, NW3 2QG, United Kingdom.
Battle
T
T
Fuller
B
B
Davidson
B
B
D000241:Adenosine; D000328:Adult; D000368:Aged; D000493:Allopurinol; D002469:Cell Separation / Q000379:methods*; D003080:Cold Temperature; D005260:Female; D005978:Glutathione; D006498:Hepatectomy; D006801:Humans; D066298:In Vitro Techniques; D007328:Insulin; D007511:Ischemia; D008099:Liver / Q000098:blood supply / Q000166:cytology*; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation; D019188:Organ Preservation Solutions; D011887:Raffinose; D013696:Temperature
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose