Effects of bFGF and TGFbeta on the expression of amyloid precursor and B-cell lymphoma protooncogene proteins in the rat retina.
The pattern of immunoreactivity for amyloid precursor (APP) and B-cell lymphoma protooncogene (Bcl-2) proteins in the rat retina was studied after intravitreal injection of basic fibroblast growth factor (bFGF) or transforming growth factor-beta (TGFbeta). In normal control retinas, intense immunostaining of APP and Bcl-2 was observed primarily in the endfeet and proximal part of radial processes of Müller glial cells. A dose-dependent reduction in immunostaining of APP and Bcl-2 in Müller cells was observed after injection of bFGF and TGFbeta. These results provide the first evidence that APP and Bcl-2 can be down-regulated by cytokines in vivo.
Chen
S T
ST
Department of Anatomy, National Cheng Kung University, Taiwan.
Jen
A
A
Gentleman
S M
SM
Jen
L S
LS
D016564:Amyloid beta-Protein Precursor / Q000008:administration & dosage / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation / Q000502:physiology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D007150:Immunohistochemistry; D007267:Injections; D016393:Lymphoma, B-Cell / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000008:administration & dosage / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D014822:Vitreous Body / Q000502:physiology
D016564:Amyloid beta-Protein Precursor; D019253:Proto-Oncogene Proteins c-bcl-2; D016212:Transforming Growth Factor beta; D016222:Fibroblast Growth Factor 2