Cloning of mouse sepiapterin reductase gene and characterization of its promoter region.
We have isolated and characterized approximately 5 kb mouse sepiapterin reductase gene (Spr) and a highly homologous pseudogene (Sprp). The authentic Spr gene is present as a single copy in the mouse genome and is composed of three exons containing the entire coding region. The primer extension experiment located the transcription initiation site in a putative pyrimidine-rich Inr element. The promoter region of the Spr gene is embedded within a CpG island. It was shown that the promoter region is devoid of distinctive TATA and CAAT boxes. Transient transfection of a series of 5' deletion derivatives of the Spr promoter showed the sequence between -83 and -51 to be essential for promoter activity. The pseudogene Sprp lacks promoter region and exon 3.
Lee
S W
SW
Department of Microbiology, Inje University, Kimhae 621-749, South Korea.
Park
I Y
IY
Hahn
Y
Y
Lee
J E
JE
Seong
C S
CS
Chung
J H
JH
Park
Y S
YS
D000429:Alcohol Oxidoreductases / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D008099:Liver / Q000201:enzymology; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D011544:Pseudogenes
D000429:Alcohol Oxidoreductases; C022630:sepiapterin reductase