title: Rapid determination of polycyclic aromatic hydrocarbons in tea infusion samples by high-performance liquid chromatography and fluorimetric detection based on solid-phase extraction.
abstract: This paper describes a method for the determination of PAHs in black, green and decaffeinated tea infusion samples. The method is based on the solid phase extraction of the PAHs using Sep-Pak vac tC-18 cartridges. The PAHs are then eluted from the cartridges with dichloromethane. Quantification and detection are carried out by HPLC with a fluorimetric detector using a program of excitation and emission wavelength pairs. Recoveries at concentration levels in the range 190-1790 ng l-1 were higher than 65% for all PAHs except dibenz[a,h]anthracene, for which it was around 54%. The mean content of PAHs was in the range 28.7-112 ng l-1 in the tea infusions, with relative standard deviations between 2 and 18% (n = 4).
authors: Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Spain.
authors: M N
authors: MN
authors: Kayali-Sayadi
authors: S
authors: S
authors: Rubio-Barroso
authors: M P
authors: MP
authors: Cuesta-Jimenez
authors: L M
authors: LM
authors: Polo-Díez
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005470:Fluorometry; D006841:Hydrocarbons, Aromatic / Q000032:analysis*; D013662:Tea / Q000737:chemistry*
chemical_list: D006841:Hydrocarbons, Aromatic; D013662:Tea