Inhibitory effects of Sudanese plant extracts on HIV-1 replication and HIV-1 protease.
Forty-eight methanol and aqueous extracts from Sudanese plants were screened for their inhibitory activity on viral replication. Nineteen extracts showed inhibitory effects on HIV-induced cytopathic effects (CPE) on MT-4 cells. The extracts were further screened against HIV-1 protease (PR) using an HPLC assay method. Of the tested extracts, the methanol extracts of Acacia nilotica (bark and pods), Euphorbia granulata (leaves), Maytenus senegalensis (stem-bark) and aqueous extracts of A. nilotica (pods) and M. senegalensis (stem-bark) showed considerable inhibitory effects against HIV-1 PR. Inhibitory principles were isolated from M. senegalensis and their activities were also discussed.
Hussein
G
G
Research Institute for Wakan-Yaku (Traditional Sino-Japanese Medicines), Toyama Medical and Pharmaceutical University, Japan.
Miyashiro
H
H
Nakamura
N
N
Hattori
M
M
Kawahata
T
T
Otake
T
T
Kakiuchi
N
N
Shimotohno
K
K
D019380:Anti-HIV Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D016333:HIV Protease / Q000378:metabolism*; D017320:HIV Protease Inhibitors / Q000494:pharmacology*; D015497:HIV-1 / Q000187:drug effects*; D006801:Humans; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D019441:Plant Epidermis / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013397:Sudan; D013634:Tannins / Q000032:analysis / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
D019380:Anti-HIV Agents; D017320:HIV Protease Inhibitors; D010636:Phenols; D010936:Plant Extracts; D013634:Tannins; D016333:HIV Protease