Human ovary and placenta express messenger RNA for multiple activin receptors.
In the present study, we examined the expression of activin receptor (ActR) mRNAs in human ovary and placenta. Primers specific for two type I and two type II activin receptors (ActR-I, ActR-IB, ActR-II, and ActR-IIB) were used in polymerase chain reaction (PCR) to amplify cDNAs prepared from granulosa-luteal cells, placental tissues and isolated trophoblast cells. PCR products with the expected sizes for ActR-I, ActR-IB, ActR-II, and ActR-IIB mRNAs were detected in freshly dissociated and 5-day cultured granulosa-luteal cells; and in trophoblast cells from both first trimester and term placentas. The identity of these PCR products were confirmed by Southern blot hybridization, as well as cloning and sequencing. These results suggest that multiple activin receptors are present in human ovary and placenta and may mediate activin function in these tissues. The demonstration of activin receptor mRNAs in granulosa-luteal and trophoblast cells further supports the notion that activin is an important local regulator in the human ovary and placenta.
Peng
C
C
Department of Biology, York University, North York, Ontario, Canada.
Ohno
T
T
Koh
L Y
LY
Chen
V T
VT
Leung
P C
PC
D029404:Activin Receptors; D001921:Brain / Q000378:metabolism; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006801:Humans; D010053:Ovary / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D012333:RNA, Messenger / Q000032:analysis*; D017978:Receptors, Growth Factor / Q000235:genetics*; D014327:Trophoblasts / Q000378:metabolism
D012333:RNA, Messenger; D017978:Receptors, Growth Factor; D029404:Activin Receptors