Transcript analysis of 250 novel yeast genes from chromosome XIV.
The European Functional Analysis Network (EUROFAN) is systematically analysing the function of novel Saccharomyces cerevisiae genes revealed by genome sequencing. As part of this effort our consortium has performed a detailed transcript analysis for 250 novel ORFs on chromosome XIV. All transcripts were quantified by Northern analysis under three quasi-steady-state conditions (exponential growth on rich fermentative, rich non-fermentative, and minimal fermentative media) and eight transient conditions (glucose derepression, glucose upshift, stationary phase, nitrogen starvation, osmo-stress, heat-shock, and two control conditions). Transcripts were detected for 82% of the 250 ORFs, and only one ORF did not yield a transcript of the expected length (YNL285w). Transcripts ranged from low (62%), moderate (16%) to high abundance (2%) relative to the ACT1 mRNA. The levels of 73% of the 206 chromosome XIV transcripts detected fluctuated in response to the transient states tested. However, only a small number responded strongly to the transients: eight ORFs were induced upon glucose upshift; five were repressed by glucose; six were induced in response to nitrogen starvation; three were induced in stationary phase; five were induced by osmo-stress; four were induced by heat-shock. These data provide useful clues about the general function of these ORFs and add to our understanding of gene regulation on a genome-wide basis.
Planta
R J
RJ
Dept. Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Brown
A J
AJ
Cadahia
J L
JL
Cerdan
M E
ME
de Jonge
M
M
Gent
M E
ME
Hayes
A
A
Kolen
C P
CP
Lombardia
L J
LJ
Sefton
M
M
Oliver
S G
SG
Thevelein
J
J
Tournu
H
H
van Delft
Y J
YJ
Verbart
D J
DJ
Winderickx
J
J
D015152:Blotting, Northern; D015825:Chromosomes, Fungal / Q000235:genetics*; D015870:Gene Expression; D005800:Genes, Fungal; D018869:Heat-Shock Response / Q000235:genetics; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D014158:Transcription, Genetic
D012331:RNA, Fungal; D012333:RNA, Messenger