Recent biotechnological developments in the use of peroxidases.
Peroxidases are ubiquitous oxidoreductases that use hydrogen peroxide or alkyl peroxides as oxidants. Advances have recently been made in using them to prepare, under mild and controlled conditions, chiral organic molecules that are valuable for the chemoenzymatic synthesis of a wide range of useful compounds. Horseradish peroxidase can be converted into a peroxygenative enzyme by molecular engineering. Chloroperoxidase, the most versatile peroxidase, behaves like a 'true' monooxygenase in sulfoxidations with molecular oxygen and an external reductant, with substantial increases in enantioselectivity and enzyme stability.
Colonna
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Centro CNR and Istituto di Chimica Organica, Facoltà di Farmacia, Università degli Studi di Milano, via Venezian 21, I-20133 Milano, Italy.Colonna@imiucca.csi.unimi.it
Gaggero
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Richelmi
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Pasta
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D000438:Alcohols / Q000737:chemistry / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D004795:Enzyme Stability; D006900:Hydroxylation; D007211:Indoles / Q000737:chemistry / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D013455:Sulfur / Q000737:chemistry / Q000378:metabolism
D000438:Alcohols; D007211:Indoles; D013455:Sulfur; D010544:Peroxidases; D009584:Nitrogen