title: Expression and distribution of adenosine diphosphate-ribosylation factors in the rat kidney.
abstract: BACKGROUND
  Adenosine diphosphate (ADP)-ribosylation factors (ARFs) are small guanosine triphosphatases involved in membrane traffic regulation. Aiming to explore the possible involvement of ARF1 and ARF6 in the reabsorptive properties of the nephron, we evaluated their distribution along the different renal epithelial segments.
  
  
  METHODS
  ARFs were detected by immunofluorescence and immunogold cytochemistry on renal sections, using specific anti-ARF antibodies.
  
  
  RESULTS
  ARF1 was detected in proximal and distal tubules, thick ascending limbs of Henle's loops, and cortical and medullary collecting ducts. By immunofluorescence, labeling was mostly localized to the cell cytoplasm, particularly in Golgi areas. By electron microscopy, the Golgi apparatus and the endosomal compartment of proximal and distal tubular cells were labeled. ARF6 immunofluorescence was observed in brush border membranes and the cytoplasm of proximal convoluted tubular cells, whereas it was restricted to the apical border of proximal straight tubules. ARF6 immunogold labeling was detected over microvilli and endocytic compartments of proximal tubular cells.
  
  
  CONCLUSIONS
  This study demonstrates the following: (a) the heterogeneous distributions of ARF1 and ARF6 along the nephron, (b) the existence of cytosolic and membrane-bound forms for both ARFs, and (c) their association with microvilli and endocytic compartments, suggesting an active participation in renal reabsorption.
authors: Department of Pathology and Cell Biology, Université de Montréal, Quebec, Canada.
authors: I
authors: I
authors: Londoño
authors: V
authors: V
authors: Marshansky
authors: S
authors: S
authors: Bourgoin
authors: P
authors: P
authors: Vinay
authors: M
authors: M
authors: Bendayan
mesh_terms: D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D000818:Animals; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D019204:GTP-Binding Proteins / Q000096:biosynthesis* / Q000276:immunology / Q000378:metabolism; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D007687:Kidney Tubules, Proximal / Q000378:metabolism / Q000648:ultrastructure; D016253:Microscopy, Immunoelectron; D051381:Rats; D014018:Tissue Distribution; D020696:rab5 GTP-Binding Proteins
chemical_list: D000911:Antibodies, Monoclonal; D019204:GTP-Binding Proteins; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; D020696:rab5 GTP-Binding Proteins