The chicken HPRT gene: a counter selectable marker for the DT40 cell line.
We describe the cloning, characterisation and chromosomal mapping of the chicken hprt gene together with the construction of two counter selectable hprt-/- DT40 derived cell lines. One of these cell lines contains a stably integrated gene encoding a conditionally active cre recombinase and thus allows efficient manipulation of targeted loci by site-specific recombination. These cell lines will enhance the utility of the hyper-recombinogenic DT40 cell line as a system for the genetic analysis of cell autonomous functions in vertebrates and as a tool for mammalian chromosome engineering.
Fukagawa
T
T
Biochemistry Department, Oxford University, South Parks Road, Oxford OX1 3QU, UK, Brunel University, Uxbridge, Middlesex UB8 3PH, UK.
Hayward
N
N
Yang
J
J
Azzalin
C
C
Griffin
D
D
Stewart
A F
AF
Brown
W
W
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002461:Cell Line, Transformed; D002645:Chickens; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D017931:DNA Primers; D005818:Genetic Engineering; D005819:Genetic Markers; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics*; D008969:Molecular Sequence Data; D011995:Recombination, Genetic; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D005819:Genetic Markers; D007041:Hypoxanthine Phosphoribosyltransferase