[Chemical modification of allergen leading to changes in its epitopic activity].
Modification of a model allergen ovalbumin (OA) with succinylation led to a decrease of its allergenicity measured by passive cutaneous anaphylaxis reaction, RAST inhibition assay and basophil histamine release. Modified OA stimulated OA-specific T-cell hybrid 3DO-548 to produce IL-2 at the same level as in case of non-modified OA. Modified OA did not induce anti-OA IgE, but did induce anti-OA IgG antibodies. This approach to chemical modification of allergen-selective blockade of B-cell epitopes while not affecting T-cell epitopes suggests new opportunities in creation of safe and effective allergovaccines.
Babakhin
A A
AA
Gushchin
I S
IS
Andreev
S M
SM
Petrukhina
A I
AI
Viler
A V
AV
Stokinger
B
B
Nolte
G
G
Dubuske
L M
LM
Khaitov
R M
RM
Petrpv
R V
RV
D000485:Allergens / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000939:Epitopes / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013268:Stimulation, Chemical
D000485:Allergens; D000939:Epitopes