Caspases: preparation and characterization.
Caspases and their involvement in programmed cell death have been an area of significant interest since their initial identification in 1992. To facilitate the search for new components involved in cell death, and to aid researchers in understanding the interactions between currently known cell death proteins, we describe a number of techniques commonly used in the preparation and characterization of caspases.
Stennicke
H R
HR
The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, California 92037, USA.
Salvesen
G S
GS
D017209:Apoptosis; D020169:Caspases / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000235:genetics; D007700:Kinetics; D011480:Protease Inhibitors / Q000494:pharmacology; D013053:Spectrophotometry; D013379:Substrate Specificity
D011480:Protease Inhibitors; D020169:Caspases