title: 20S proteasome, hsp90, p97 fusion protein, PA28 activator copurifying oligomers and ATPase activities.
abstract: Whether hsp90 acts in an ATP-dependent or independent way is of crucial importance for understanding the molecular mechanism of this chaperone and, to day, the involvement of ATP hydrolysis in hsp90 function is still a controversial subject. ATPase activities may be detected in partially purified hsp90's preparations from rabbit muscle. We demonstrate that the major contaminant associated with hsp90 is the p97 fusion protein and that these oligomeric structures are copurifying together with the 20S proteasome and its PA28 activator. Improving the purification procedure permits to separate hsp90 and p97 to homogeneity. Then, our attempts failed to detect any significant ATPase activity in the hsp90 fraction. Thus, p97 would be principally responsible for the ATPase activity detected in partially purified hsp90 preparations from rabbit muscle.
authors: ISTAB, Laboratoire de Biochimie et Technologie des Aliments, Université Bordeaux I, Talence, France.
authors: V
authors: V
authors: Montel
authors: F
authors: F
authors: Gardrat
authors: J L
authors: JL
authors: Azanza
authors: J
authors: J
authors: Raymond
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D003546:Cysteine Endopeptidases / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000737:chemistry*; D009132:Muscles / Q000201:enzymology / Q000378:metabolism; D046988:Proteasome Endopeptidase Complex; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits
chemical_list: D018841:HSP90 Heat-Shock Proteins; D008565:Membrane Proteins; D009097:Multienzyme Complexes; D011506:Proteins; D003546:Cysteine Endopeptidases; D046988:Proteasome Endopeptidase Complex; D000251:Adenosine Triphosphatases