Use of RAPD-PCR fingerprinting to detect genetic diversity of soil Streptomyces isolates.
Random amplified polymorphic DNA (RAPD) was used for identification and assessment of genetic diversity between isolates of Streptomyces from soil. Genomic DNA from 18 Streptomyces isolates and 2 reference strains were amplified using four different 10-mer primers. Different DNA fingerprinting patterns were obtained for all the isolates. Electrophoretic and cluster analysis of the amplification products revealed incidence of polymorphism among the isolates and none of them was identical to the reference strains although there were some common amplification bands. Two highly divergent groups were determined among the isolates. The results indicate that RAPD is an efficient method for discriminating and studying genetic diversity of Streptomyces isolates.
Malkawi
H I
HI
Yarmouk University, Department of Biological Sciences, Irbid-Jordan.
Saadoun
I
I
Moumani
F A
FA
Meqdam
M M
MM
D004269:DNA, Bacterial / Q000032:analysis; D014644:Genetic Variation; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000145:classification*; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics*
D004269:DNA, Bacterial