Synthesis of 2'-C-alpha-difluoromethylarauridine and its 3'-O-phosphoramidite incorporation into a hammerhead ribozyme.
The 2'-C-difluoromethylated nucleoside 4 was synthesized starting from uridine. 4 was then converted to the 3'-O-phosphoramidite derivative 5 and was incorporated into a hammerhead ribozyme (7). The cleavage characteristics of the modified oligonucleotide have been analysed.
Dunkel
M
M
Innovir GmbH, Rosdorf, Germany. mdunkel@nigu.de
Reither
V
V
D000818:Animals; D001483:Base Sequence; D002384:Catalysis; D004795:Enzyme Stability / Q000187:drug effects; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016337:RNA, Catalytic / Q000737:chemistry / Q000187:drug effects* / Q000378:metabolism; D012754:Sharks; D014529:Uridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology
C119132:2'-C-difluoromethylarauridine; C119134:2'-C-difluoromethylarauridine 3'-O-phosphoramidite; D016337:RNA, Catalytic; D014529:Uridine