title: Anti-inflammatory activity of flavone and some of its derivates from Virola michelli Heckel.
abstract: The phytochemical study using Virola michelli Heckel (Myristicaceae) leaves allowed the isolation of a flavone named titonine (7,4'-dimethoxy-3'-hydroxyflavone). Titonine was further submitted to methylation and acetylation reactions yielding a 7,3',4'-trimethoxyflavone and a 7,3'-dimethoxy-4'-acetylflavone, respectively. These compounds were evaluated for both anti-inflammatory and analgesic activity. The anti-inflammatory activity was evaluated in rats using the paw edema test with carrageenin, while the analgesic activity was determined in mouse using the writhing test method. The different animal groups were treated with three compounds (10 mg/kg -i.p.) thirty min prior to stimuli application. The inhibition levels obtained for each compound were 22, 41 and 68%, respectively. Using the writhing test, oral doses of 5, 10 and 15 mg/kg of natural flavone reduced the acetic acid-induced contortions in a dose-dependent manner.
authors: Laboratório de Fitofármacos, Instituto de Farmácia e Nutrição, Universidade de Alfenas, UNIFENAS, Minas Gerais, Brazil. jctc@artefinal.com.br
authors: J C
authors: JC
authors: Carvalho
authors: L P
authors: LP
authors: Ferreira
authors: L
authors: L
authors: da Silva Santos
authors: M J
authors: MJ
authors: Corrêa
authors: L M
authors: LM
authors: de Oliveira Campos
authors: J K
authors: JK
authors: Bastos
authors: S J
authors: SJ
authors: Sarti
mesh_terms: D000700:Analgesics / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D002351:Carrageenan; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy*; D047309:Flavones; D005419:Flavonoids / Q000302:isolation & purification / Q000627:therapeutic use*; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D051379:Mice; D010147:Pain Measurement / Q000187:drug effects; D010946:Plants, Medicinal; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D047309:Flavones; D005419:Flavonoids; D002351:Carrageenan; C043562:flavone