title: A ribosomal orphon sequence from Xenopus laevis flanked by novel low copy number repetitive elements.
abstract: We have used a differential cloning approach to isolate ribosomal/non-ribosomal frontier sequences from Xenopus laevis. A ribosomal intergenic spacer sequence (IGS) was cloned and shown not to be physically linked with the ribosomal locus. This ribosomal orphon contained the IGS sequences found immediately downstream of the 28S gene and included an array of enhancer repetitions and a non-functional spacer promoter. The orphon sequence was flanked by a member of the novel 'Frt' low copy repetitive element family. Three individual Frt repeats were sequenced and all members of this family were shown to lie clustered at two chromosomal sites, one of which contained the ribosomal orphon. One of the Frt elements contained an insertion of 297 bp that showed extensive homology to sequences within at least three other Xenopus genes. Each homology region was flanked by members of the T2 family of short interspersed repetitive elements, (SINEs), and by its target insertion sequence, suggesting multiple translocation events. The data are discussed in terms of the evolution of the ribosomal gene locus.
authors: Centre de Recherche en Cancérologie et Départment de Biologie médicale l'Université Laval, Hôtel-Dieu de Québec, Canada.
authors: A
authors: A
authors: Guimond
authors: T
authors: T
authors: Moss
mesh_terms: D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary; D004275:DNA, Ribosomal; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D012091:Repetitive Sequences, Nucleic Acid; D012270:Ribosomes; D012689:Sequence Homology, Nucleic Acid; D014982:Xenopus laevis / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D004275:DNA, Ribosomal