Impact of various glycosaminoglycans upon cAMP-dependent protein kinase.
The physiological effects of the second messenger cAMP are displayed by cAMP-dependent protein kinase-medicated phosphorylation of specific target proteins which in turn control diverse cellular functions. We have determined this enzyme substrate phosphorylation in the presence of various glycosaminoglycans using a cAMP-dependent protein kinase isolated from rat liver. The results indicate that sulfated and unsulfated polysaccharides are able to inhibit phosphorylation of histone type IIa catalysed by cAMP-dependent protein kinase. Based on their impact upon substrate phosphorylation, glycosaminoglycans can be divided into three groups: group I with the highest inhibitory effect: dermatan sulfate and heparan sulfate; group II: chondroitin 4-sulfate and group III with the lowest inhibitory effect: chondroitin 6-sulfate, keratan sulfate and hyaluronic acid.
Dittmann
J
J
Institut für Physiologische Chemie, Tierärztliche Hochschule Hannover, Germany. jdittman@biochemie.tiho-hannover.de
Krischek
C
C
Harisch
G
G
D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism*; D003871:Dermatan Sulfate / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006025:Glycosaminoglycans / Q000378:metabolism / Q000494:pharmacology*; D006497:Heparan Sulfate / Q000494:pharmacology; D006657:Histones / Q000378:metabolism; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D010766:Phosphorylation; D051381:Rats; D017208:Rats, Wistar; D015290:Second Messenger Systems / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D013379:Substrate Specificity
D002809:Chondroitin Sulfates; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006657:Histones; D006820:Hyaluronic Acid