Dieldrin reduces male production and sex ratio in Daphnia galeata mendotae.
We used the sex ratio of neonate Daphnia, as well as the more standard endpoints of adult survivorship and fecundity and neonatal morphology, as an assay for detecting the effects of the insecticides endosulfan and dieldrin. Dieldrin caused a decrease in sex ratio (number of males/number of males plus females); we observed no endosulfan effect. We estimated (by extrapolation) that the sex ratio was reduced by dieldrin from concentrations of about 30 ppb and higher, based on a linear decrease in sex ratio with log dieldrin concentrations from 50 to 600 ppb. Neither insecticide significantly affected adult survival or clutch size. Because sex ratio changed but total neonate production did not change, the data suggest that the effect of dieldrin was on the sex-determining system during embryogenesis. Neither insecticide caused morphological abnormalities. Mixtures of the two pesticides produced only additive effects.
Dodson
S I
SI
Department of Zoology, University of Wisconsin, Madison 53706-1381, USA. sidodson@facstaff.wisc.edu
Merritt
C M
CM
Torrentera
L
L
Winter
K M
KM
Tornehl
C K
CK
Girvin
K
K
D000818:Animals; D003621:Daphnia / Q000187:drug effects* / Q000196:embryology; D004026:Dieldrin / Q000494:pharmacology / Q000633:toxicity*; D005260:Female; D007306:Insecticides / Q000494:pharmacology / Q000633:toxicity*; D008297:Male; D012733:Sex Differentiation / Q000187:drug effects*; D012744:Sex Ratio; D014874:Water Pollutants, Chemical / Q000494:pharmacology / Q000633:toxicity
D007306:Insecticides; D014874:Water Pollutants, Chemical; D004026:Dieldrin