Frequency of HLA-A and B alleles in early and late-onset Alzheimer's disease.
The frequency of various allele types of the class I Major Histocompatibility Complex (MHC) genes HLA-A and HLA-B were compared between pathologically confirmed groups of late and early-onset Alzheimer's disease (AD) and a control group. DNA was extracted from frozen brain tissue and the highly polymorphic second and third exons of the HLA-A and HLA-B genes were independently PCR amplified using specific primers. Individual allele types were identified using sequence-specific oligonucleotide probes. The results showed that the main frequency differences occurred between the late-onset AD and the control group however none of these reached statistical significance.
Middleton
D
D
Northern Ireland Histocompatibility and Immunogenetics Laboratory, Belfast City Hospital, UK.
Mawhinney
H
H
Curran
M D
MD
Edwardson
J A
JA
Perry
R
R
McKeith
I
I
Morris
C
C
Ince
P G
PG
Neill
D
D
D000328:Adult; D017668:Age of Onset; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000544:Alzheimer Disease / Q000453:epidemiology / Q000235:genetics*; D001921:Brain / Q000473:pathology; D005091:Exons / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D015234:HLA-A Antigens / Q000235:genetics*; D015235:HLA-B Antigens / Q000235:genetics*; D006801:Humans; D008297:Male; D016555:Matched-Pair Analysis; D008875:Middle Aged; D015345:Oligonucleotide Probes; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D013223:Statistics as Topic
D015234:HLA-A Antigens; D015235:HLA-B Antigens; D015345:Oligonucleotide Probes