Molecular cloning and expression of Xenopus laevis requiem cDNA1.
Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Krüppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.
Konishi
M
M
Departments of Anatomy, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Hiraoka
Y
Y
Ogawa
M
M
Sakai
Y
Y
Ishii
H
H
Aiso
S
S
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000302:isolation & purification; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D016415:Sequence Alignment; D014157:Transcription Factors; D014982:Xenopus laevis / Q000235:genetics*
D018076:DNA, Complementary; D004268:DNA-Binding Proteins; C113581:DPF2 protein, human; D014157:Transcription Factors