Isolation and sequence of the GCR3 homologue from Candida albicans by complementation of (delta)gcr3 strain of Saccharomyces cerevisiae.
To study the function of GCR3, a gene involved in the expression of glycolytic genes in Saccharomyces cerevisiae, a Candida albicans gene which complements the growth defect of the (delta)gcr3 mutant was isolated. Transformants of this gene also recovered the glycolytic enzyme activities. Its DNA sequencing predicted an 886 amino acid protein with 30.4% identity to the Gcr3p of S. cerevisiae.
Uemura
H
H
Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki, Japan. uemura@nibh.go.jp
Nakamoto
K
K
Sugioka
S
S
Tadenuma
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D002176:Candida albicans / Q000235:genetics*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics*; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D039381:RNA Cap-Binding Proteins; D015183:Restriction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D005656:Fungal Proteins; D009687:Nuclear Proteins; D039381:RNA Cap-Binding Proteins; C101398:STO1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins