title: Leupeptin protects sensory hair cells from acoustic trauma.
abstract: Calpains, a family of calcium activated proteases, promote the breakdown of cellular proteins, kinases, phosphatases and transcription factors. Calpain inhibitors attenuate some neurodegenerative processes in certain cell types. Here we show that leupeptin, a potent calpain inhibitor, protects the sensory hair cells in the inner ear from acoustic overstimulation (48 h, 100 or 105 dB SPL, octave band noise at 4 kHz). Acoustic overstimulation caused a significant increase in calpain immunolabeling in the sensory epithelium suggesting a possible role in noise-induced cochlear degeneration. Infusion of leupeptin into the inner ear significantly reduced the amount of sensory cell loss from acoustic overstimulation. However, leupeptin did not protect against hair cell loss from the ototoxic drug, carboplatin.
authors: Hearing Research Lab, SUNY University at Buffalo, NY 14214, USA.
authors: J
authors: J
authors: Wang
authors: D
authors: D
authors: Ding
authors: A
authors: A
authors: Shulman
authors: A
authors: A
authors: Stracher
authors: R J
authors: RJ
authors: Salvi
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000037:antagonists & inhibitors / Q000633:toxicity; D002154:Calpain / Q000037:antagonists & inhibitors*; D016190:Carboplatin / Q000037:antagonists & inhibitors / Q000633:toxicity; D002682:Chinchilla; D004791:Enzyme Inhibitors / Q000627:therapeutic use*; D006198:Hair Cells, Auditory / Q000473:pathology*; D006311:Hearing Disorders / Q000139:chemically induced / Q000517:prevention & control; D006317:Hearing Loss, Noise-Induced / Q000473:pathology* / Q000517:prevention & control*; D007150:Immunohistochemistry; D007976:Leupeptins / Q000627:therapeutic use*; D009925:Organ of Corti / Q000502:physiology
chemical_list: D000970:Antineoplastic Agents; D004791:Enzyme Inhibitors; D007976:Leupeptins; D016190:Carboplatin; D002154:Calpain; C032854:leupeptin