title: Determination of the stoichiometry and strength of binding of xanthophylls to the photosystem II light harvesting complexes.
abstract: Xanthophylls have a crucial role in the structure and function of the light harvesting complexes of photosystem II (LHCII) in plants. The binding of xanthophylls to LHCII has been investigated, particularly with respect to the xanthophyll cycle carotenoids violaxanthin and zeaxanthin. It was found that most of the violaxanthin pool was loosely bound to the major complex and could be removed by mild detergent treatment. Gentle solubilization of photosystem II particles and thylakoids allowed the isolation of complexes, including a newly described oligomeric preparation, enriched in trimers, that retained all of the in vivo violaxanthin pool. It was estimated that each LHCII monomer can bind at least one violaxanthin. The extent to which different pigments can be removed from LHCII indicated that the relative strength of binding was chlorophyll b > neoxanthin > chlorophyll a > lutein > zeaxanthin > violaxanthin. The xanthophyll binding sites are of two types: internal sites binding lutein and peripheral sites binding neoxanthin and violaxanthin. In CP29, a minor LHCII, both a lutein site and the neoxanthin site can be occupied by violaxanthin. Upon activation of the violaxanthin de-epoxidase, the highest de-epoxidation state was found for the main LHCII component and the lowest for CP29, suggesting that only violaxanthin loosely bound to LHCII is available for de-epoxidation.
authors: Robert Hill Institute, Department of Molecular Biology & Biotechnology, University of Sheffield, Western Bank, Sheffield S10 2TN, United Kingdom.
authors: A V
authors: AV
authors: Ruban
authors: P J
authors: PJ
authors: Lee
authors: M
authors: M
authors: Wentworth
authors: A J
authors: AJ
authors: Young
authors: P
authors: P
authors: Horton
mesh_terms: D001665:Binding Sites; D002338:Carotenoids / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D045342:Light-Harvesting Protein Complexes; D014975:Lutein / Q000378:metabolism*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D045332:Photosystem II Protein Complex; D010860:Pigments, Biological / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013054:Spectrophotometry, Atomic; D024341:Xanthophylls; D019207:beta Carotene / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: C069660:CP29 light harvesting complex; D045342:Light-Harvesting Protein Complexes; D045322:Photosynthetic Reaction Center Complex Proteins; D045332:Photosystem II Protein Complex; D010860:Pigments, Biological; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C005613:violaxanthin; D014975:Lutein