title: Identification of a highly promiscuous and an HLA allele-specific T-cell epitope in the birch major allergen Bet v 1: HLA restriction, epitope mapping and TCR sequence comparisons.
abstract: BACKGROUND
  Allergen-specific CD4+ T cells play an important regulatory role in atopic allergy.
  
  
  OBJECTIVE
  To investigate the human leucocyte antigen (HLA) restriction and T-cell receptor (TCR) usage of allergen-specific T-cell clones (TCCs) that react with defined epitopes of Bet v 1, the major birch pollen allergen.
  
  
  METHODS
  Five Bet v 1-specific TCCs derived from two birch pollen-allergic individuals and specific for Bet v 1, were epitope-mapped with overlapping synthetic peptides. In addition, HLA-restriction and TCR CDR3 sequences were determined.
  
  
  RESULTS
  Three TCCs reacted with a Bet v 1 peptide containing amino acid residues 21-33 (BP21), the other two TCCs reacted with a minimal peptide comprising residues 37-45 (BP37). Studies using neutralizing anti-HLA-monoclonal antibodies and HLA-typed APCs showed that the BP37-specific TCCs were restricted by a HLA-DQA1*0301/DQB1*0603 heterodimer. In contrast, BP21 was recognized in a highly promiscuous manner. TCCs recognizing this sequence were restricted by HLA-DPB1*0201, a HLA-DQA1*0201/DQB1*0201 heterodimer, or HLA-DRB3*0101. Reverse transcription-polymerase chain reaction with primers for all known TCRAV and TCRBV gene segments, followed by CDR3 region sequencing, revealed the usage of five different TCRAV and four different TCRBV gene segments by the TCCs, as well as diversity in the joining region. All BP21-specific TCCs contained a negatively charged residue in their CDR3alpha regions, the CDR3beta regions showed a high concentration of polar and OH-group bearing residues. BP37-specific TCCs shared the amino acid combination LY in the middle of their CDR3alpha regions, the CDR3beta regions showed high concentration of OH-group bearing or charged residues.
  
  
  CONCLUSIONS
  This study shows the existence of a highly promiscuous T-cell epitope in Bet v 1. The presence of additional T-cell epitopes in Bet v 1 may, however, hamper the clinical applicability of the epitope. Likewise, the diversity in TCR usage by T cells recognizing the epitope does not support the development of TCR-directed immunotherapy for birch pollen allergy.
authors: Department of General and Experimental Pathology, Vienna, Austria.
authors: R
authors: R
authors: Friedl-Hajek
authors: M D
authors: MD
authors: Spangfort
authors: C
authors: C
authors: Schou
authors: H
authors: H
authors: Breiteneder
authors: H
authors: H
authors: Yssel
authors: R J
authors: RJ
authors: Joost van Neerven
mesh_terms: D000483:Alleles; D000485:Allergens / Q000276:immunology*; D017951:Antigen Presentation; D052179:Antigens, Plant; D001483:Base Sequence; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte; D005802:Genes, MHC Class II; D006801:Humans; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000276:immunology*; D011948:Receptors, Antigen, T-Cell / Q000737:chemistry*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D018984:Epitopes, T-Lymphocyte; D010940:Plant Proteins; D011948:Receptors, Antigen, T-Cell; C061312:Bet v 1 allergen, Betula