title: The role of the tumor suppressor p53 in spermatogenesis.
abstract: The p53 protein appeared to be involved in both spermatogonial cell proliferation and radiation response. During normal spermatogenesis in the mouse, spermatogonia do not express p53, as analyzed by immunohistochemistry. However, after a dose of 4 Gy of X-rays, a distinct p53 staining was present in spermatogonia, suggesting that, in contrast to other reports, p53 does have a role in spermatogonia. To determine the possible role of p53 in spermatogonia, histological analysis was performed in testes of both p53 knock out C57BL/6 and FvB mice. The results indicate that p53 is an important factor in normal spermatogonial cell production as well as in the regulation of apoptosis after DNA damage. First, p53 knock out mouse testes contained about 50% higher numbers of A1 spermatogonia, indicating that the production of differentiating type spermatogonia by the undifferentiated spermatogonia is enhanced in these mice. Second, 10 days after a dose of 5 Gy of X-rays, in the p53 knock out testes, increased numbers of giant sized spermatogonial stem cells were found, indicating disturbance of the apoptotic process in these cells. Third, in the p53 knock out testis, the differentiating A2-B spermatogonia are more radioresistant compared to their wild-type controls, indicating that p53 is partly indispensable in the removal of lethally irradiated differentiating type spermatogonia. In accordance with our immunohistochemical data, Western analysis showed that levels of p53 are increased in total adult testis lysates after irradiation. These data show that p53 is important in the regulation of cell production during normal spermatogenesis either by regulation of cell proliferation or, more likely, by regulating the apoptotic process in spermatogonia. Furthermore, after irradiation, p53 is important in the removal of lethally damaged spermatogonia.
authors: Department of Cell Biology, Medical School, P.O. Box 80.157, 3508 TD Utrecht, The Netherlands. T.L.Beumer@MED.RUU.NL
authors: T L
authors: TL
authors: Beumer
authors: H L
authors: HL
authors: Roepers-Gajadien
authors: I S
authors: IS
authors: Gademan
authors: P P
authors: PP
authors: van Buul
authors: G
authors: G
authors: Gil-Gomez
authors: D H
authors: DH
authors: Rutgers
authors: D G
authors: DG
authors: de Rooij
mesh_terms: D000818:Animals; D002452:Cell Count; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000528:radiation effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology*; D014965:X-Rays
chemical_list: D016159:Tumor Suppressor Protein p53