title: Kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen.
abstract: The kinetics of the interaction of rabbit skeletal muscle phosphorylase kinase with glycogen was studied by the turbidimetric method at pH 6.8 and 8.2. Binding of phosphorylase kinase by glycogen occurs only in the presence of Ca2+ and Mg2+. The initial rate of complex formation is proportional to the enzyme and polysaccharide concentration; this suggests the formation of a complex with 1:1 stoichiometry in the initial step of phosphorylase kinase binding by glycogen. The kinetic data suggest that phosphorylase kinase substrate--glycogen phosphorylase b--favors the binding of phosphorylase kinase with glycogen. This conclusion is supported by direct experiments on the influence of phosphorylase b on the interaction of phosphorylase kinase with glycogen using analytical sedimentation analysis. The kinetic curves of the formation of the complex of phosphorylase kinase with glycogen obtained in the presence of ATP are characterized by a lag period. Preincubation of phosphorylase kinase with ATP in the presence of Ca2+ and Mg2+ causes the complete disappearance of the lag period. On changing the pH from 6.8 to 8.2, the rate of phosphorylase kinase binding by glycogen is appreciably increased, and complex formation becomes possible even in the absence of Mg2+. A model of phosphorylase kinase and phosphorylase b adsorption on the surface of the glycogen particle explaining the increase in the strength of phosphorylase kinase binding with glycogen in the presence of phosphorylase b is proposed.
authors: Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, 117071, Russia.
authors: I E
authors: IE
authors: Andreeva
authors: V F
authors: VF
authors: Makeeva
authors: B I
authors: BI
authors: Kurganov
authors: N A
authors: NA
authors: Chebotareva
authors: N B
authors: NB
authors: Livanova
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008112:Liver Glycogen / Q000378:metabolism*; D008274:Magnesium / Q000378:metabolism; D018482:Muscle, Skeletal / Q000201:enzymology* / Q000378:metabolism; D010764:Phosphorylase Kinase / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D013552:Swine
chemical_list: D008112:Liver Glycogen; D000255:Adenosine Triphosphate; D010764:Phosphorylase Kinase; D008274:Magnesium; D002118:Calcium