title: Eosinophil derived neurotoxin (EDN) levels in commercial human urinary preparations of glycoprotein hormones.
abstract: Eosinophil derived neurotoxin (EDN) is a ubiquitous human ribonuclease, occurring not only in eosinophils, but also in many tissues and body fluids. It may be a contaminant of commercial human urinary preparations of chorionic gonadotropin (hCG) and other glycoprotein hormones. Here we describe the use of a fast commercial assay to quantify this contaminant and demonstrate that the content varies much between different commercial glycoprotein hormone preparations. As this ribonuclease may have a cytotoxic activity on certain cells, it is useful to be able to determine its quantity in a fast and reliable way in these preparations.
authors: Dept. of Allergology, University Hospital Groningen, Netherlands.
authors: H F
authors: HF
authors: Kauffman
authors: H
authors: H
authors: Hovenga
authors: H W
authors: HW
authors: de Bruijn
authors: J J
authors: JJ
authors: Beintema
mesh_terms: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents / Q000032:analysis*; D000998:Antiviral Agents / Q000032:analysis*; D006063:Chorionic Gonadotropin / Q000652:urine; D047208:Eosinophil-Derived Neurotoxin; D005640:Follicle Stimulating Hormone / Q000652:urine; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007986:Luteinizing Hormone / Q000652:urine; D011506:Proteins / Q000032:analysis*; D011863:Radioimmunoassay; D012259:Ribonuclease, Pancreatic / Q000032:analysis; D012260:Ribonucleases
chemical_list: D000911:Antibodies, Monoclonal; D000970:Antineoplastic Agents; D000998:Antiviral Agents; D006063:Chorionic Gonadotropin; D006023:Glycoproteins; D011506:Proteins; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D047208:Eosinophil-Derived Neurotoxin; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic