title: Evidence that a single replication fork proceeds from early to late replicating domains in the IgH locus in a non-B cell line.
abstract: In non-B cell lines, like the murine erythroleukemia cell line (MEL), the most distal IgH constant region gene, C alpha, replicates early in S; other heavy chain constant region genes, joining and diversity segments, and the most proximal Vh gene replicate successively later in S in a 3' to 5' direction proportional to their distance from C alpha. In MEL, replication forks detected in the IgH locus also proceed in the same 3' to 5' direction for approximately 400 kb, beginning downstream of the IgH 3' regulatory region and continuing to the D region, as well as within the Vh81X gene. Downstream of the initiation region is an early replicating domain, and upstream of Vh81X is a late replicating domain. Hence, the gradual transition between early and late replicated domains can be achieved by a single replication fork.
authors: Department of Cell Biology, Albert Einstein College of Medicine, New York, New York 10461, USA.
authors: O V
authors: OV
authors: Ermakova
authors: L H
authors: LH
authors: Nguyen
authors: R D
authors: RD
authors: Little
authors: C
authors: C
authors: Chevillard
authors: R
authors: R
authors: Riblet
authors: N
authors: N
authors: Ashouian
authors: B K
authors: BK
authors: Birshtein
authors: C L
authors: CL
authors: Schildkraut
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism; D015139:Blotting, Southern; D004261:DNA Replication; D015180:Electrophoresis, Gel, Two-Dimensional; D005091:Exons / Q000235:genetics; D005803:Genes, Immunoglobulin / Q000235:genetics*; D007127:Immunoglobulin Constant Regions / Q000235:genetics; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007133:Immunoglobulin Joining Region / Q000235:genetics; D007135:Immunoglobulin Variable Region / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D012093:Replicon / Q000235:genetics*; D016196:S Phase; D013698:Templates, Genetic; D013997:Time Factors; D014407:Tumor Cells, Cultured
chemical_list: D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007133:Immunoglobulin Joining Region; D007135:Immunoglobulin Variable Region