Buffer effect on the kinetics of ornithine carbamyl transferase by HPLC.
A kinetic study of ornithine carbamyl transferase (OCT) from Streptococcus faecalis has been carried out, employing a new HPLC method based on the direct determination of citrulline. A different kinetic pattern was observed in tris(hydroxymethyl)-aminomethane (TRIS) and triethanolamine (TEA) buffers: a ping-pong or an ordered sequential mechanism are suggested, respectively. The inhibition of Tris buffer on OCT reaction has also been demonstrated. The inhibition of Tris buffer on OCT reaction has also been demonstrated.
Carunchio
V
V
Dipartimento di Chimica, Università di Roma La Sapienza, Rome, Italy.
Girelli
A M
AM
Messina
A
A
D002021:Buffers; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D013293:Enterococcus faecalis / Q000201:enzymology; D007700:Kinetics; D009954:Ornithine Carbamoyltransferase / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet
D002021:Buffers; D009954:Ornithine Carbamoyltransferase