Assay for endogenous heparin in plasma of livestock using a synthetic chromogenic substrate.
The levels of endogenous heparin in the plasmas of horses, cows, sheep and pigs were determined with the use of synthetic chromogenic substrate benzoyl-isoleucyl-glutamyl-glycyl-arginyl-p-nitroanilide (S-2222). The lowest heparin concentrations were stated in cattle plasma, the highest ones in the plasma of pigs.
Ledwozyw
A
A
Department of Pathophysiology, Agricultural University, Lublin, Poland.
Jabłonka
S
S
Tusińska
E
E
Herbut
M
M
D000818:Animals; D002417:Cattle / Q000097:blood*; D003124:Colorimetry / Q000379:methods / Q000662:veterinary; D015951:Factor Xa / Q000737:chemistry; D006405:Hematology / Q000379:methods; D006493:Heparin / Q000097:blood* / Q000737:chemistry*; D006736:Horses / Q000097:blood*; D009842:Oligopeptides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012756:Sheep / Q000097:blood*; D013045:Species Specificity; D013053:Spectrophotometry / Q000379:methods / Q000662:veterinary; D013552:Swine / Q000097:blood*
D009842:Oligopeptides; C015608:benzoyl-Ile-Glu-Gly-Arg-p-nitroanilide; D006493:Heparin; D015951:Factor Xa