Influence of age and plasma treatment on neutrophil phagocytosis and CD18 expression in foals.
The aim of this study was to evaluate the influence of age and plasma treatment on neutrophil phagocytosis, CD18 expression and serum opsonic capacity in foals in field settings. Microbial infections constitute a large threat in young foals and neutrophil functions are crucial for the defense. Blood samples were obtained from 13 foals at seven time points between the ages of 2 and 56 days and once from 16 adult horses. Six of the foals were treated with adult plasma at the age of 1 week. Neutrophil phagocytosis of yeast after various opsonizations and the expression of complement adhesion receptor CD18 were analysed by flow cytometry. Autologous serum opsonization resulted in 52+/-6.1% phagocytic neutrophils in 2-day-old foals (n = 12), a significantly lower rate than in adult horses (mean 84+/-3.1%; n = 16). In foals, yeast ingestion per neutrophil was also lower than in adults. Opsonic capacity increased with age (p < 0.05), reaching adult levels at 3-4 weeks. An increase in serum opsonic capacity followed plasma treatment (p < 0.05). The phagocytic capacity of foal neutrophils at the time-points studied was equal to or higher than that in the adults, when pooled adult horse serum or anti-yeast IgG was used as opsonin. In foals, serum IgG concentration was negatively correlated to serum opsonic capacity. CD18 receptor expression was higher in neutrophils from foals (<21 days old) than in those from adult horses (p < 0.05). The results indicate that foals are transiently deficient in serum opsonic capacity, which negatively affects their capacity for neutrophil phagocytosis. These changes in serum opsonins, unrelated to IgG, may be important factors in susceptibility to infections in foals.
Gröndahl
G
G
Department of Large Animal Clinical Sciences, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, Uppsala. gittan.grondahl@kirmed.slu.se
Johannisson
A
A
Demmers
S
S
Jensen Waern
M
M
D000193:Actinomycetales Infections / Q000517:prevention & control; D000367:Age Factors; D000818:Animals; D018821:CD18 Antigens / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000662:veterinary; D005786:Gene Expression Regulation; D006734:Horse Diseases / Q000276:immunology*; D006736:Horses; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D009895:Opsonin Proteins / Q000097:blood / Q000276:immunology; D010587:Phagocytosis / Q000276:immunology*; D010949:Plasma / Q000276:immunology; D012240:Rhodococcus / Q000276:immunology; D012441:Saccharomyces cerevisiae / Q000276:immunology
D018821:CD18 Antigens; D007074:Immunoglobulin G; D009895:Opsonin Proteins