Effects of low ozone-oxygen concentrations on the acetylcholine release at the mouse neuromuscular junction.
The use of a mixture of low concentrations of ozone (O3) with oxygen (O2) have been proved to be useful in different human pathological conditions. Owing to a lack of both pharmacological and epidemiological basic studies, the scientific consideration of this therapeutic potential is still inappropriate. Here, we started, from an electrophysiological point of view, a study on the possible effects of low O3 doses on the acetylcholine (ACh) release at the neuromuscular junction. Indeed, some experimental conditions indicate a positive effect either in maintaining cytosolic calcium (Ca2+) homeostasis or in increasing the efficacy of the intracellular antioxidant systems. Furthermore, a positive action on the kinetics of some antioxidant enzymes must be taken into account as a possible molecular mechanism in the regulation of the function of cellular homeostasis. Our data demonstrate a reduction of evoked ACh release in the mouse neuromuscular junction. O3 affects neither the spontaneous ACh release nor the kinetics of the ACh-receptor-channel complex. The results are compatible with a reduction of intracellular Ca2+ and proved a molecular action of O3.
Re
L
L
Institute of Experimental and Clinical Medicine, Laboratory of Pharmacology, University of Ancona, Italy. lambertore@popcsi.unian.it
Valli
A
A
Barocci
S
S
Giammarchi
V
V
Serresi
M
M
Faini
T
T
Ricci
M
M
Morales
M A
MA
León-Fernández
O S
OS
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D004594:Electrophysiology / Q000379:methods; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000378:metabolism; D010100:Oxygen / Q000494:pharmacology*; D010126:Ozone / Q000494:pharmacology*
D010126:Ozone; D000109:Acetylcholine; D010100:Oxygen