Decreased sensory neuropeptide release from trachea of rats with streptozotocin-induced diabetes.
We studied the release of somatostatin, calcitonin gene-related peptide (CGRP) and substance P in response to electrical field stimulation from isolated tracheas of rats following 4 weeks of streptozotocin (50 mg/kg i.v.)-induced diabetes. Field stimulation (40 V, 0.1 ms, 10 Hz for 120 s) increased the release of somatostatin, CGRP and substance P from the baseline 0.18+/-0.029, 0.17+/-0.027, and 1.77+/-0.086 to 0.51+/-0.022, 0.69+/-0.115, and 5.96+/-0.377 in control preparations and 0.31+/-0.081, 0.41+/-0.142, and 3.14+/-0.443 fmol/mg wet tissue weight in preparations from diabetic rats as measured by radioimmunoassay (control vs. diabetic P<0.01 for each). The results show a simultaneous decrease in release of the three sensory neuropeptides and an enhanced plasma somatostatin level in rats with streptozotocin-induced diabetes.
Nemeth
J
J
Neuroscience Research Group, University Medical School of Pecs, Hungary. jnemeth@apacs.pote.hu
Szilvassy
Z
Z
Than
M
M
Oroszi
G
G
Sari
R
R
Szolcsanyi
J
J
D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D015740:Calcitonin Gene-Related Peptide / Q000097:blood / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004558:Electric Stimulation; D066298:In Vitro Techniques; D008297:Male; D009479:Neuropeptides / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013004:Somatostatin / Q000097:blood / Q000378:metabolism*; D013373:Substance P / Q000097:blood / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*
D001786:Blood Glucose; D009479:Neuropeptides; D013373:Substance P; D013004:Somatostatin; D015740:Calcitonin Gene-Related Peptide