An in vivo study of antifreeze protein adjuvant cryosurgery.
Cryosurgery employs freezing to destroy undesirable tissue. However, under certain thermal conditions, frozen tissues survive. The survival of frozen undesirable tissue may lead to complications, such as recurrence of cancer. In a study of nude mice with subcutaneous metastatic prostate tumors, we showed that the preoperative injection of a phosphate-buffered saline solution with 10 mg/ml antifreeze protein of type I into the tumor prior to freezing enhances destruction under thermal conditions which normally yield cell survival. This suggests that the adjunctive use of antifreeze proteins in cryosurgery may reduce the complications from undesirable tissues that survive freezing.
Pham
L
L
Bioengineering Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California 94720, USA.
Dahiya
R
R
Rubinsky
B
B
D000818:Animals; D021301:Antifreeze Proteins; D002470:Cell Survival; D003452:Cryosurgery / Q000295:instrumentation / Q000379:methods*; D006023:Glycoproteins / Q000008:administration & dosage*; D006801:Humans; D015552:Injections, Intralesional; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009374:Neoplasms, Experimental / Q000473:pathology / Q000556:secondary / Q000601:surgery*; D011471:Prostatic Neoplasms / Q000473:pathology / Q000601:surgery; D014407:Tumor Cells, Cultured
D021301:Antifreeze Proteins; D006023:Glycoproteins