A comparative analysis of the citrate permease P mRNA stability in Lactococcus lactis biovar diacetylactis and Escherichia coli.
The role of ribonucleases in the control of gene expression remains unknown in lactic acid bacteria. In the present work, we analysed the expression of the citP gene, which encodes the lactococcal citrate permease P, through the stability of the citQRP messenger in both Lactococcus lactis biovar diacetylactis (L. diacetylactis) and Escherichia coli. The chemical half-life for citQRP mRNA observed in L. diacetylactis wild-type strain was abnormally long for bacteria. It was even longer than that detected in E. coli RNase E or RNase III mutant strains. A model of processing and fate of RNA species containing citP gene is presented.
Drider
D
D
Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
García-Quintáns
N
N
Santos
J M
JM
Arraiano
C M
CM
López
P
P
D001426:Bacterial Proteins; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D006207:Half-Life; D007218:Industrial Microbiology; D013294:Lactococcus lactis / Q000201:enzymology* / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D027361:Organic Anion Transporters; D012329:RNA, Bacterial / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism*; D012260:Ribonucleases / Q000494:pharmacology
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D027361:Organic Anion Transporters; D012329:RNA, Bacterial; D012333:RNA, Messenger; C065431:citP protein, Lactococcus lactis; D012260:Ribonucleases