The expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) and its regulation by ovarian steroids in rat uterine stromal cells.
The effects of ovarian steroids on the expression of granulocyte-macrophage colony-stimulating factor (GM-CSF) in rat uterus were examined. Intense expression of GM-CSF mRNA was dispersedly located in the endometrial-myometrial junction and stroma of the uterus. GM-CSF was immunohistochemically localized in stromal cells and luminal epithelium. Ovariectomy significantly reduced the appearance of GM-CSF-mRNA-positive cells and the levels of expression for GM-CSF mRNA in the whole uterus, whereas treatment with 17beta-estradiol (E2) or a combination of E2 and progesterone (P4) for 5 days on ovariectomized animals recruited GM-CSF-mRNA-positive cells and stimulated its expression. The combined treatment with E2 and P4 also stimulates the expression for GM-CSF mRNA and the production of immunoreactive GM-CSF in the stromal tissues. These data suggest that the expression of GM-CSF in uterine stromal cells is partially regulated by ovarian steroids.
Tamura
K
K
Department of Pharmacology, Tokyo University of Pharmacy & Life Science, Hachioji, Japan.
Kumasaka
K
K
Kogo
H
H
D000818:Animals; D004848:Epithelium / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics / Q000378:metabolism*; D007120:Immunochemistry; D010052:Ovariectomy; D010053:Ovary / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D051381:Rats; D017154:Stromal Cells / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D011374:Progesterone; D004958:Estradiol; D016178:Granulocyte-Macrophage Colony-Stimulating Factor