Determination of glycarbylamide in chicken tissue by high-performance liquid chromatography.
A high-performance liquid chromatographic (HPLC) method for determining glycarbylamide (GB) in chicken tissue was developed. GB was extracted with acetonitrile, followed by solid-phase extraction cleanup using a Bond Elut cartridge column with neutral alumina. After the extract had been evaporated to dryness, the residue was dissolved in 1.0 mL 0.1 N sodium hydroxide. Then 1.0 mL 0.1 M potassium dihydrogen phosphate solution was added to it. HPLC separation was done on a 250 x 4.6 mm id TSK-GEL ODS 80 column with 0.05M potassium dihydrogen phosphate as the mobile phase. Ultraviolet detection was done at a wavelength of 260 nm. The calibration curve of standard GB solutions was linear between 0.16 and 3 micrograms/mL (correlation coefficient, r = 0.999). The recovery of GB from chicken muscle spiked at 0.8 microgram/g was 88.6 +/- 2.3% (mean +/- standard deviation, n = 5), and the lower limit of determination was 0.05 microgram/g in chicken muscle.
Yamamoto
Y
Y
Miyazaki University, Faculty of Agriculture, Department of Veterinary Public Health, Miyazaki-shi, Japan.
Kondo
F
F
D000097:Acetonitriles; D000537:Aluminum Oxide; D000818:Animals; D002645:Chickens; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003049:Coccidiostats / Q000032:analysis*; D005506:Food Contamination; D007093:Imidazoles / Q000032:analysis*; D009132:Muscles / Q000737:chemistry*; D012997:Solvents
D000097:Acetonitriles; D003049:Coccidiostats; D007093:Imidazoles; D012997:Solvents; C005006:glycarbylamide; D000537:Aluminum Oxide; C032159:acetonitrile