Effects of interferon-alpha on cellular proliferation and adhesion of breast carcinoma cells.
We examined the potent inhibitory effects of interferon-alpha (IFN-alpha) on both cellular adhesion and cell proliferation of MCF-7 breast carcinoma cells. When MCF-7 cells were exposed to IFN-alpha at a concentration of 5x10(3) IU/ml for 5 days, cell proliferation was markedly inhibited. Cell attachment assay demonstrated that incubation with IFN-alpha for up to 48 h reduced alpha2beta1 integrin-mediated cellular adhesion. However, fluorescence activated cell sorter (FACS) analysis revealed that incubation with IFN-alpha for 24 h had no effect upon the cell surface expressions of either alpha2 and beta1 integrin on MCF-7 cells. These antiproliferative and antiadhesive actions of IFN-alpha may be applied to treatment for patients with metastatic breast carcinoma.
Maemura
M
M
Second Department of Surgery, Gunma University School of Medicine, Maebashi, Gunma 371-8511, Japan.
Iino
Y
Y
Horiguchi
J
J
Takei
H
H
Horii
Y
Y
Koibuchi
Y
Y
Yokoe
T
T
Takeyoshi
I
I
Ohwada
S
S
Morishita
Y
Y
D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002455:Cell Division / Q000187:drug effects; D006801:Humans; D016023:Integrins / Q000502:physiology; D007370:Interferon Type I / Q000494:pharmacology*; D038981:Receptors, Collagen; D011994:Recombinant Proteins; D014407:Tumor Cells, Cultured
D016023:Integrins; D007370:Interferon Type I; D038981:Receptors, Collagen; D011994:Recombinant Proteins