Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor.
The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water-organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5-50 mM.
Lukachova
L V
LV
Faculty of Chemistry, M. V. Lomonosov Moscow State University, Russia.
Karyakin
A A
AA
Ivanova
Y N
YN
Karyakina
E E
EE
Varfolomeyev
S D
SD
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004867:Equipment Design; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase
D005949:Glucose Oxidase; D005947:Glucose