title: In vivo 13C-NMR studies of polymer synthesis in rhizobium meliloti M5N1 strain
abstract: The use of in vivo 13C-NMR approach for the monitoring of the synthesis of various polymers within cells of Rhizobium meliloti (M5N1 strain) is reported. Significant differences in polymer biosynthesis have been shown as a function of the metabolic state of the cells and the labeled carbon source used. Consumption of carbon source and produced glycogen was complete with mid-exponential phase harvested cells. This was not the case with stationary phase harvested cells, for which polyhydroxybutyrate synthesis was higher and gluconate synthesis was lower than the former. [1-13C]fructose-grown cells produced more exopolysaccharide and polyhydroxybutyrate, but less beta-(1,2) glucan and gluconate than [1-13C]glucose-grown cells. This approach offers a suitable tool to examine the kinetics of polymer biosynthesis by Rhizobia. Copyright 1998 John Wiley & Sons, Inc.
authors: Laboratoire de Genie Cellulaire UPRES-A CNRS 6022, Faculte des Sciences, Universite de Picardie Jules Verne, 33, rue Saint-Leu, 80039 Amiens Cedex, France.
authors: P
authors: Tavernier
authors: I
authors: Besson
authors: JC
authors: Portais
authors: J
authors: Courtois
authors: B
authors: Courtois
authors: JN
authors: Barbotin