Expression of mRNA encoding alpha1E and alpha1G subunit in the brain of a rat model of absence epilepsy.
Low voltage-activated calcium channels are thought to play a key role in the generation of spike and waves discharges characteristic of absence epilepsy. Therefore, the expression level of mRNA encoding calcium channel alpha1E and alpha1G subunits was measured in the brain of genetic absence epilepsy rats from Strasbourg (GAERS). Using quantitative RT-PCR and in situ hybridization, no difference was found in alpha1G mRNA expression between GAERS and control animals, while a decreased expression of alpha1E was seen in the cerebellum and the brain stem of the GAERS. This phenomenon was not observed in young animals when the epileptic phenotype is not expressed.
de Borman
B
B
Laboratory of Biochemistry, Research Group in Neurochemistry, University of Liege, Belgium.
Lakaye
B
B
Minet
A
A
Zorzi
W
W
Vergnes
M
M
Marescaux
C
C
Grisar
T
T
D000818:Animals; D001933:Brain Stem / Q000378:metabolism; D015220:Calcium Channels / Q000235:genetics*; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D002531:Cerebellum / Q000378:metabolism; D004832:Epilepsy, Absence / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D007536:Isomerism; D008297:Male; D009419:Nerve Tissue Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains / Q000235:genetics; D017208:Rats, Wistar; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction
C498917:Cacna1e protein, rat; D015220:Calcium Channels; D020908:Calcium Channels, R-Type; D027682:Cation Transport Proteins; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger