title: Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines.
abstract: We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate vaccine construction. The selected polysaccharide fragments can be used to produce glycoconjugate vaccines containing a restricted saccharide polydispersion. We have applied this chromatographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The method is easily adapted for manufacturing purposes.
authors: Chiron Vaccines SpA, Siena, Italy.
authors: P
authors: P
authors: Costantino
authors: F
authors: F
authors: Norelli
authors: A
authors: A
authors: Giannozzi
authors: S
authors: S
authors: D'Ascenzi
authors: A
authors: A
authors: Bartoloni
authors: S
authors: S
authors: Kaur
authors: D
authors: D
authors: Tang
authors: R
authors: R
authors: Seid
authors: S
authors: S
authors: Viti
authors: R
authors: R
authors: Paffetti
authors: M
authors: M
authors: Bigio
authors: C
authors: C
authors: Pennatini
authors: G
authors: G
authors: Averani
authors: V
authors: V
authors: Guarnieri
authors: E
authors: E
authors: Gallo
authors: N
authors: N
authors: Ravenscroft
authors: C
authors: C
authors: Lazzeroni
authors: R
authors: R
authors: Rappuoli
authors: C
authors: C
authors: Ceccarini
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry*; D016667:Bacterial Capsules / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D018073:Haemophilus Vaccines / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry; D009345:Neisseria meningitidis; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D014462:Ultrafiltration; D018074:Vaccines, Conjugate / Q000737:chemistry*
chemical_list: D000942:Antigens, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; C037676:meningococcal group C polysaccharide