title: Recombinant human leukemia inhibitory factor enhances the development of preimplantation mouse embryo in vitro.
abstract: OBJECTIVE
  To assess the effect of recombinant human leukemia inhibitory factor (rhLIF) on mouse embryos in vitro.
  
  
  METHODS
  Controlled prospective study.
  
  
  METHODS
  Academic research environment.
  
  
  METHODS
  Female CB6F1 mice between 6 and 8 weeks old.
  
  
  METHODS
  Mice were divided randomly into three groups, which included a control group in an in vivo study (group I) and two groups in an in vitro study (groups II and III). Mice were killed at 116-120 hours (group I) and 44-48 hours (groups II and III) after hCG injection. Two-cell embryos (groups II and III) and blastocysts (group I) were obtained. Embryos in group II were cocultured with human tubal fluid (HTF) + 0.5% human serum albumin and in group III with HTF + rhLIF (1,000 U/mL) under paraffin oil.
  
  
  METHODS
  The embryonic numbers in different stages were recorded and compared.
  
  
  RESULTS
  Similar early embryo development to the four- to eight-cell and morula stages was noted between groups II and III (87.3% versus 91.0% and 74.6% versus 87.1%, respectively). However, further embryo development to the blastocyst, expanded blastocyst, and hatching blastocyst in group II (48.1%, 31.7%, and 18.5%, respectively) was lower than that in group III (83.6%, 53.7%, and 37.8%).
  
  
  CONCLUSIONS
  RhLIF does not provide obvious stimulation in the early mouse embryo. However, rhLIF has positive effects on preimplantation blastocyst growth, differentiation, and hatching.
authors: Department of Obstetrics and Gynecology, China Medical College Hospital, China Medical College, Taichung, Taiwan. D3531@HPD.CMCH.ORG.TW
authors: H D
authors: HD
authors: Tsai
authors: C C
authors: CC
authors: Chang
authors: Y Y
authors: YY
authors: Hsieh
authors: H Y
authors: HY
authors: Lo
authors: L W
authors: LW
authors: Hsu
authors: S C
authors: SC
authors: Chang
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000502:physiology; D002455:Cell Division; D047108:Embryonic Development; D005314:Embryonic and Fetal Development; D005260:Female; D006131:Growth Inhibitors / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6; D053605:Leukemia Inhibitory Factor; D008222:Lymphokines / Q000494:pharmacology*; D051379:Mice; D009028:Morula / Q000502:physiology; D011247:Pregnancy; D011446:Prospective Studies; D011994:Recombinant Proteins / Q000494:pharmacology; D014327:Trophoblasts / Q000166:cytology
chemical_list: D006131:Growth Inhibitors; D015850:Interleukin-6; C507837:LIF protein, human; D053605:Leukemia Inhibitory Factor; C507838:Lif protein, mouse; D008222:Lymphokines; D011994:Recombinant Proteins