Capillary GC-MS determination of melatonin in several pharmaceutical tablet formulations.
A simple capillary GC-MS method for the determination of melatonin from tablets is proposed. Melatonin (N-acetyl-5-methoxytryptamine) was determined by gas chromatography-mass spectrometry after extraction from ground tablets with ethyl acetate and derivatization with N-methyl-N-trimethylsilyl-heptafluorobutyramide (MTSHFBA). Splitless injection offers good reproducibility with a standard deviation of +/- 2%. The proposed method was applied to analyse the melatonin content from three different tablet formulations.
Aboul-Enein
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HY
Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.
Doneanu
C
C
Covaci
A
A
D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D008550:Melatonin / Q000032:analysis*; D013607:Tablets / Q000737:chemistry*
D013607:Tablets; D008550:Melatonin