[Vibrio cholerae temperate phage O139: characteristics and role in changing expression of chromosomal virulence genes].
Restriction analysis of temperate cholera phage 139 isolated from Vibrio cholerae P16064, serogroup 0139, showed its DNA to be double-stranded linear with cohesive terminals. DNA-DNA hybridization on nylon membranes revealed that many V. cholerae strains of serogroup 0139 isolated in different regions contained a temperate cholera phage 139 in their genomes. Southern blot hybridization of chromosomal DNA PST-fragments with phage probe showed that the temperate phage 139 was identical to the temperate phage of serogroup II V. eltor. The phage integrated in the chromosome near genes encoding motility (mot) and production of the capsule (cap) and purine (pur). Phage genome is apparently responsible for instability of cap, pur, and mot genes whose products are important for the development of an infectious process in cholera.
Smirnova
N I
NI
Eroshenko
G A
GA
Shchelkanova
E Iu
EIu
Livanova
L F
LF
Konnov
N P
NP
D001435:Bacteriophages / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D015139:Blotting, Southern; D002876:Chromosomes, Bacterial; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D005798:Genes, Bacterial; D016679:Genome, Viral; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization; D014734:Vibrio cholerae / Q000235:genetics / Q000472:pathogenicity / Q000821:virology*; D014774:Virulence / Q000235:genetics*; D016662:Virus Integration