Nucleus extraction from single mounted tissue sections.
Extraction of nuclei from unmounted archived tissue has become a method widely used for molecular cytogenetic investigations. It is a suitable approach to take advantage of the large series of formalin fixed and subsequently paraffin-embedded material which has been collected in the times before interphase cytogenetic analysis has become possible. We present a new kind of assay for the extraction of nuclei from one single mounted tissue section; the extracted interphase nuclei are suitable very well for a fluorescence in situ hybridization (FISH) approach. The method described has been successfully used for the analysis of the INT2/FGF3-amplicon in 20 samples of oral squamous cell carcinoma.
Liehr
T
T
Institute of Human Genetics, Jena, Germany. i8lith@mti-n.mti.uni-jena.de
Uwe
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C
Erich
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G
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology; D002457:Cell Extracts; D002467:Cell Nucleus; D051518:Fibroblast Growth Factor 3; D005346:Fibroblast Growth Factors / Q000235:genetics; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008867:Microtomy; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology; D011518:Proto-Oncogene Proteins / Q000235:genetics
D002457:Cell Extracts; C496444:FGF3 protein, human; D051518:Fibroblast Growth Factor 3; D011518:Proto-Oncogene Proteins; D005346:Fibroblast Growth Factors