Cellular uptake and delivery monitoring of liposome/DNA complexes during in vitro transfection of CFTR gene.
The cellular uptake and distribution of cationic liposomes Dc-Chol/DOPECFTR gene complexes were assessed by electronic and confocal laser scanner microscopy (CLSM) for the CFTR gene transfer to human adenocarcinoma and tracheal epithelial cell lines. Cationic lipid forms unilamellar and multilamellar vesicles capable of rapid and efficient transport of gene into target cells. The number of fluorescent complexes was increasing with time in cells up to 6 hours showing a punctate and homogeneous DNA distribution in the cytoplasmatic and nuclear compartments, including the nucleolus. No significant difference in the biochemical and cellular behavior was observed between the investigated system and other systems previously tested. This study adds new insights into the CFTR cationic liposome-mediated gene delivery.
Serafino
A L
AL
Istituto di Medicina Sperimentale C.N.R., Roma, Italy.
Novelli
G
G
Di Sario
S
S
Colosimo
A
A
Amicucci
P
P
Sangiuolo
F
F
Mossa
G
G
Dallapiccola
B
B
D002460:Cell Line; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics* / Q000378:metabolism; D018736:DNA Adducts / Q000737:chemistry; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D014162:Transfection / Q000379:methods*
C505032:CFTR protein, human; D018736:DNA Adducts; D008081:Liposomes; D019005:Cystic Fibrosis Transmembrane Conductance Regulator