Evaluation of the stability and immunogenicity of autoclaved and nonautoclaved preparations of a vaccine against American tegumentary leishmaniasis.
This study was designed to evaluate the immunogenicity of autoclaved and nonautoclaved preparations of a vaccine composed of whole antigens from killed promastigotes of Leishmania amazonensis. Leishmanin skin-test (LST)-negative volunteers were immunized with either autoclaved or nonautoclaved vaccine preparations (32 and 36 subjects, respectively) that had been maintained at 4 degrees C for one year before the onset of this trial. Immunological tests were performed two days before and 40 days after vaccination. The LST conversion rates induced by the autoclaved and nonautoclaved vaccines were significantly different: 59% and 83%, respectively. Leishmania antigen-stimulated proliferative responses of peripheral blood mononuclear cells (PBMC) were significantly higher after vaccination than before vaccination in both groups. The CD8+ subset was predominant over the CD4+ subset among the leishmania-reactive cells after vaccination in both groups. The production of IFN-gamma by the leishmania antigen-stimulated PBMC was significantly higher after vaccination than before vaccination in the group receiving the nonautoclaved vaccine but not in the autoclaved vaccine group. IL-2 was found both before and after vaccination with no differences between its levels in these time points in either group. IL-4 was not detected for either group during the study period.
De Luca
P M
PM
Departamento de Protozoologia, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro-RJ, Brasil.
Mayrink
W
W
Alves
C R
CR
Coutinho
S G
SG
Oliveira
M P
MP
Bertho
A L
AL
Toledo
V P
VP
Costa
C A
CA
Genaro
O
O
Mendonça
S C
SC
D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D007892:Leishmania braziliensis / Q000276:immunology; D016773:Leishmaniasis, Cutaneous / Q000517:prevention & control*; D008297:Male; D010641:Phenotype; D016052:Protozoan Vaccines / Q000276:immunology*; D012882:Skin Tests; D013242:Sterilization
D000953:Antigens, Protozoan; D007376:Interleukin-2; D016052:Protozoan Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma