Diagnosis of canine leishmaniasis by a polymerase chain reaction technique.
A polymerase chain reaction (PCR) technique for the diagnosis of canine leishmaniasis on bone marrow samples was developed which amplified a 120 bp DNA fragment of the Leishmania kinetoplast DNA, common to all Leishmania species. Forty-five of 46 dogs in which leishmaniasis had been diagnosed were positive with the PCR technique, whereas none of 41 healthy dogs gave a positive result. Fifteen dogs with leishmaniasis that had been treated for six months with N-methylglucamine antimoniate and allopurinol were also investigated. Seven were positive, implying that they remained infected despite the resolution of their clinical signs.
Roura
X
X
Veterinary Teaching Hospital, Facultat de Veterinària, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Sánchez
A
A
Ferrer
L
L
D000595:Amino Acid Sequence; D000818:Animals; D016054:DNA, Protozoan / Q000032:analysis*; D003937:Diagnosis, Differential; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D007896:Leishmaniasis / Q000175:diagnosis / Q000662:veterinary*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D016054:DNA, Protozoan