Cutting edge: LFA-1 is required for liver NK1.1+TCR alpha beta+ cell development: evidence that liver NK1.1+TCR alpha beta+ cells originate from multiple pathways.
Using mice deficient for LFA-1, CD44, and ICAM-1, we examined the role of these adhesion molecules in NK1.1+TCR alpha beta+ (NKT) cell development. Although no defect in NKT cell development was observed in CD44-/- and ICAM-1-/- mice, a dramatic reduction of liver NKT cells was observed in LFA-1-/- mice. Normal numbers of NKT cells were present in other lymphoid organs in LFA-1-/- mice. When LFA-1-/- splenocytes were injected i.v. into wild-type mice, the frequency of NKT cells among donor-derived cells in the recipient liver was normal. In contrast, when LFA-1-/- bone marrow (BM) cells were injected i.v. into irradiated wild-type mice, the frequency of liver NKT cells was significantly lower than that of mice injected with wild-type BM cells. Collectively, these data indicate that LFA-1 is required for the development of liver NKT cells, rather than the migration to and/or subsequent establishment of mature NKT cells in the liver.
Ohteki
T
T
Department of Medical Biophysics, Ontario Cancer Institute, Toronto, Canada. tohteki@sun.microb.med.keio.ac.jp
Maki
C
C
Koyasu
S
S
Mak
T W
TW
Ohashi
P S
PS
D000818:Animals; D000941:Antigens / Q000378:metabolism*; D000950:Antigens, Ly; D000951:Antigens, Neoplasm / Q000378:metabolism; D000954:Antigens, Surface; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D018960:Hyaluronan Receptors / Q000235:genetics / Q000502:physiology; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000502:physiology; D037181:Lectins, C-Type; D008099:Liver / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D016169:Lymphocyte Function-Associated Antigen-1 / Q000235:genetics / Q000502:physiology*; D008221:Lymphoid Tissue / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000378:metabolism*; D055630:Receptors, NK Cell Lectin-Like; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D000950:Antigens, Ly; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D002352:Carrier Proteins; D018960:Hyaluronan Receptors; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D016169:Lymphocyte Function-Associated Antigen-1; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011506:Proteins; D016693:Receptors, Antigen, T-Cell, alpha-beta; D055630:Receptors, NK Cell Lectin-Like; C052646:sarcoma glycoprotein gp96 rejection antigens; D018799:Intercellular Adhesion Molecule-1