A novel glycosylphosphatidyl inositol-anchored protein on human leukocytes: a possible role for regulation of neutrophil adherence and migration.
We report here a novel glycosylphosphatidyl-inositol (GPI)-anchored glycoprotein on human leukocytes. Treatment of neutrophils with a mAb (3H9) to this molecule sequentially up-regulates and down-regulates beta2 integrin-dependent adhesion of these cells as well as their transendothelial migration in vitro. In addition, this mAb simultaneously modulates the avidity of beta2 integrin for its ligand, iC3b, with kinetics similar to those observed in 3H9 modulation of neutrophil adherence. This mAb also induces beta2 integrin-dependent cytoskeletal remodeling. This novel GPI-anchored protein (GPI-80) is highly homologous with Vanin-1, a recently reported GPI-anchored protein that is expressed on perivascular thymic stromal cells and is involved in thymus homing in mice. The finding that both GPI-80 and Vanin-1 are 40% homologous with human biotinidase suggests the existence of a biotinidase superfamily of molecules that may be involved in the regulation of leukocyte trafficking.
Suzuki
K
K
Department of Immunology and Parasitology, Yamagata University School of Medicine, Japan, USA.
Watanabe
T
T
Sakurai
S
S
Ohtake
K
K
Kinoshita
T
T
Araki
A
A
Fujita
T
T
Takei
H
H
Takeda
Y
Y
Sato
Y
Y
Yamashita
T
T
Araki
Y
Y
Sendo
F
F
D000199:Actins / Q000097:blood; D000276:Adjuvants, Immunologic / Q000502:physiology; D000581:Amidohydrolases; D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000097:blood / Q000502:physiology; D001483:Base Sequence; D001666:Binding Sites, Antibody; D001667:Binding, Competitive / Q000276:immunology; D018821:CD18 Antigens / Q000097:blood; D002448:Cell Adhesion / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D002465:Cell Movement / Q000276:immunology*; D003179:Complement C3b / Q000378:metabolism; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols / Q000097:blood*; D006801:Humans; D006867:Hydrolases; D008562:Membrane Glycoproteins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D051379:Mice; D008969:Molecular Sequence Data; D009504:Neutrophils / Q000276:immunology*; D017386:Sequence Homology, Amino Acid
D000199:Actins; D000276:Adjuvants, Immunologic; D000911:Antibodies, Monoclonal; D018821:CD18 Antigens; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; D017261:Glycosylphosphatidylinositols; D008562:Membrane Glycoproteins; D003179:Complement C3b; D006867:Hydrolases; D000581:Amidohydrolases; C121771:VNN2 protein, human