High level expression of soluble angiogenin in Escherichia coli.
Human angiogenin was genetically engineered and contained the E. coli Omp A signal sequence for secreting soluble angiogenin to the periplasm under tac promoter control. The angiogenin sequence was encoded in a single gene and expressed as a 14.4 kilodalton soluble protein in E. coli. It was purified by CM-Sepharose ion-exchange chromatography and by a heparin-Sepharose affinity chromatography procedure. The biological activity of angiogenin was established by its ability to inhibit mRNA-dependent rabbit reticulocyte cell-free translation.
Yoon
J M
JM
Department of Microbial Chemistry, College of Pharmacy, Seoul National University, Kwanak-gu.
Kim
S H
SH
Kwon
O B
OB
Han
S H
SH
Kim
B K
BK
D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D005818:Genetic Engineering; D006801:Humans; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D011506:Proteins / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000302:isolation & purification; D012156:Reticulocytes / Q000378:metabolism; D012259:Ribonuclease, Pancreatic; D012995:Solubility
D001425:Bacterial Outer Membrane Proteins; D011506:Proteins; D011994:Recombinant Proteins; C078167:OMPA outer membrane proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic