title: Do damaged proteins accumulate in Caenorhabditis elegans L-isoaspartate methyltransferase (pcm-1) deletion mutants?
abstract: The protein l-isoaspartate (d-aspartate) O-methyltransferase (E.C. 2. 1.1.77) can initiate the conversion of isomerized and racemized aspartyl residues to their normal l-aspartyl forms and has therefore been hypothesized to function as a repair enzyme, responsible for helping to limit the accumulation of damaged proteins in aging organisms. In this study, the effect of a disruption in the pcm-1 gene encoding the l-isoaspartyl methyltransferase was investigated in the nematode Caenorhabditis elegans. It was found that damaged proteins recognized by this enzyme accumulated to significant levels during long-term incubation of both pcm-1+ and pcm-1- nematodes in a specialized larval stage called the dauer. The l-isoaspartyl methyltransferase-deficient mutants accumulated about twice the level of damaged proteins as the control nematodes during dauer aging. The mutants also accumulated higher levels of damage when both strains were incubated at 30 degrees C for up to 3 days. However, when nonviable nematodes were removed in a Percoll separation, similar levels of damage were measured between the two strains following both dauer aging and 30 degrees C incubation. Both strains were able to effectively eliminate damaged proteins recognized by the methyltransferase after recovery from dauer. Characterization of the methyl-accepting polypeptide substrates which accumulate in aged dauers revealed that although substrates of all molecular weights are present, the majority of substrates are peptides not precipitated by acetone. These results suggest that protein degradation, rather than repair, may be the major mechanism by which C. elegans eliminates damaged proteins containing l-isoaspartyl residues.
authors: Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California, 90095-1569, USA.
authors: A
authors: A
authors: Niewmierzycka
authors: S
authors: S
authors: Clarke
mesh_terms: D000096:Acetone / Q000378:metabolism; D000375:Aging / Q000502:physiology*; D000818:Animals; D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D017173:Caenorhabditis elegans / Q000235:genetics / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D002457:Cell Extracts / Q000502:physiology; D002498:Centrifugation; D003600:Cytosol / Q000201:enzymology; D017353:Gene Deletion; D006358:Hot Temperature; D008780:Methyltransferases; D013379:Substrate Specificity
chemical_list: D001324:Autoantigens; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; D002457:Cell Extracts; D000096:Acetone; C490112:pcm-1 protein, C elegans; D008780:Methyltransferases