Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization.
A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA), and were determined using GC/NCI-MS and GC/NCI-MS/MS. The linear correlation coefficients for calibration in NCI-MS/MS were in the range 0.9880-0.9988. This method of derivatization with HFBA for use with GC/NCI was useful in determinations of 19-norandrosterone, boldenone, 19-noretiocholanolone, 2-methylandrosterone, nandrolone, 1-methyleneandrosterone, 1-methylandrosterone, 4-dihydroboldenone and mesterolone. The detection limits of this procedure were 5-20 ppb at a signal-to-noise (S/N) ratio of 3.
Choi
M H
MH
Doping Control Centre, Korea Institute of Science and Technology, Cheongryang, Seoul, Korea.
Chung
B C
BC
Lee
W
W
Lee
U C
UC
Kim
Y
Y
D045930:Anabolic Agents / Q000652:urine*; D005466:Fluorocarbons; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007202:Indicators and Reagents; D015813:Substance Abuse Detection / Q000379:methods*
D045930:Anabolic Agents; D005466:Fluorocarbons; D007202:Indicators and Reagents; C007924:heptafluorobutyric anhydride