Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature.
We localized five rpoC (beta') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed beta' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved beta' residues participate in interactions holding RNA polymerase together.
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Waksman Institute and Department of Genetics, Rutgers, The State University, Piscataway, New Jersey 08854, USA.
Markov
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Naryshkina
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Severinov
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D000595:Amino Acid Sequence; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
C108570:beta' subunit of RNA polymerase; D012321:DNA-Directed RNA Polymerases