title: Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida.
abstract: CUG, a universal leucine codon, was reported to be read as serine in 10 species of the genus Candida. We used an in vitro cell-free translation system to identify the amino acid assignment of codon CUG in 78 species and 7 varieties of galactose-lacking Candida species equipped with Q9 as the major ubiquinone. Of these, only 11 species used codon CUG as a leucine codon. The remaining species decoded CUG as serine. Their small subunit ribosomal DNA sequences were also determined and analyzed using both Neighbor-Joining and Maximum Likelihood methods. The species decoding CUG as serine and leucine formed distinct clusters on both molecular phylogenetic trees. Our result suggests that non-universal decoding is not a rare event, and that it is widely distributed in the genus Candida.
authors: Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. sugita@my-pharm.ac.jp
authors: T
authors: T
authors: Sugita
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authors: T
authors: Nakase
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics; D003062:Codon; D004275:DNA, Ribosomal / Q000737:chemistry; D010802:Phylogeny; D014176:Protein Biosynthesis; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D003062:Codon; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S