Presence of an intrapineal circadian oscillator in the teleostean family Poeciliidae.
In most fish, rhythmic melatonin production is controlled by circadian oscillators located within the pineal (=pineal clocks) that are reset daily by the ambient light:dark (LD) cycle. However, one question that has yet to be addressed concerns the phylogenetic distribution of the pineal clock within fish families. We tested whether a pineal clock identified in the sailfin molly (Poecilia velifera) in an earlier study is also present in some other representatives of the teleostean family Poeciliidae. Isolated pineals from adults belonging to the genus Poecilia (P. velifera albino, P. reticulata, and P. sphenops), genus Xiphophorus (X. helleri and X. maculatus), and genus Limia (L. vittata) were obtained and cultured under LD and/or continuous darkness (DD) at constant temperature (27 degrees C). With one exception, free-running rhythms in melatonin release with circadian periodicities ranging from 19.5 to 27.4 h (n = 26) were detected in isolated pineals from all poeciliid representatives tested under DD exposure. In addition, rhythmic melatonin production was also observed in isolated pineals of some representatives tested from all three genera under LD exposure, suggesting the property of direct photosensitivity. Taken together, these data suggest that a circadian oscillator residing in the pineal of the sailfin molly also appears to be present in all of the poeciliid representatives tested in our system, supporting the notion that the presence of a pineal clock occurs at the family level of taxonomic organization.
Okimoto
D K
DK
Department of Biological Sciences, University of Delaware, Newark, Delaware, 19716, USA.
Stetson
M H
MH
D000818:Animals; D002940:Circadian Rhythm / Q000502:physiology*; D046508:Culture Techniques; D003532:Cyprinodontiformes / Q000502:physiology*; D008550:Melatonin / Q000032:analysis; D010802:Phylogeny; D010870:Pineal Gland / Q000502:physiology*; D011036:Poecilia / Q000502:physiology*; D011863:Radioimmunoassay
D008550:Melatonin