High-performance liquid chromatographic analysis of nitrite and nitrate in human plasma as S-nitroso-N-acetylcysteine with ultraviolet absorbance detection.
A rapid HPLC method with UV absorbance detection at 333 nm for the measurement of nitrite and nitrate in ultrafiltrate samples of human plasma is described. The method is based on hydrochloric acid-catalyzed conversion of nitrite by N-acetyl-L-cysteine to S-nitroso-N-acetyl-L-cysteine and isocratic elution using 10 mM NaH2PO4 in acetonitrile-water, pH 2.0 (15:85, v/v). The limit of detection of the method is 50 nM nitrite. The method was validated by gas chromatography-mass spectrometry.
Tsikas
D
D
Institute of Clinical Pharmacology, Hannover Medical School, Germany. tsikas.dimitros@mh-hannover.de
Rossa
S
S
Sandmann
J
J
Frölich
J C
JC
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D009566:Nitrates / Q000097:blood*; D009573:Nitrites / Q000097:blood*; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet
D009566:Nitrates; D009573:Nitrites; C024940:S-nitroso-N-acetylcysteine; D000111:Acetylcysteine