title: Non-aqueous enzymology approach for improvement of reagentless mediator-based glucose biosensor.
abstract: The formation of Nafion membranes containing glucose oxidase and dimethylferrocene as a mediator was optimized using a previously reported non-aqueous enzymology approach for biosensor development. Enzyme immobilization in Nafion membranes was carried out from water-organic mixtures with a high content of organic solvent. The mediator based reagentless glucose electrode was tested in a flow injection system. The response towards glucose addition was stable: the reproducibility during 50 assays exceeded 95%. The response was linear over the glucose concentration range 0.5-50 mM.
authors: Faculty of Chemistry, M. V. Lomonosov Moscow State University, Russia.
authors: L V
authors: LV
authors: Lukachova
authors: A A
authors: AA
authors: Karyakin
authors: Y N
authors: YN
authors: Ivanova
authors: E E
authors: EE
authors: Karyakina
authors: S D
authors: SD
authors: Varfolomeyev
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004563:Electrochemistry; D004867:Equipment Design; D017022:Flow Injection Analysis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase
chemical_list: D005949:Glucose Oxidase; D005947:Glucose