title: An interlaboratory study to determine the presence of human papillomavirus DNA in esophageal carcinoma from China.
abstract: Esophageal-carcinoma samples originating from the high-incidence area of China were tested in 2 different laboratories, each using a different degenerate PCR approach. Results confirmed the notion that none of the PCR approaches available for HPV-DNA detection today, is optimal for detecting all known HPV types at equal sensitivity and specificity. In combining results obtained in both laboratories, HPV DNA was demonstrated in 20/117 (17.1%) esophageal-carcinoma samples analyzed. HPV DNA was detected in 3/70 (4.3%) diagnostic biopsies, 7/23 (30.4%) surgical specimen and 10/24 (41.6%) cytological scrapings originating from the entire surface of the esophagus. Mucosotropic HPV types were present in 7/117 (6%) samples, only 3 being of the high-risk types (HPV 16, 18, 33). Other mucosal types found were HPV 6, 11, 13, 53 and 54. Cutaneous HPV types were present in 14/117 (12.0%) samples. HPVs 20 and 38 were present in 3 (2.6%) of the total samples and, in each case, together with another HPV type within one lesion. Two putative new HPV types, DL347 and DL 369, were identified.
authors: Division for Tumour-Virus Characterization, Deutsches Krebsforschungszentrum, Heidelberg, Germany. e.devilliers@dkfz-heidelberg.de
authors: E M
authors: EM
authors: de Villiers
authors: D
authors: D
authors: Lavergne
authors: F
authors: F
authors: Chang
authors: K
authors: K
authors: Syrjänen
authors: P
authors: P
authors: Tosi
authors: M
authors: M
authors: Cintorino
authors: R
authors: R
authors: Santopietro
authors: S
authors: S
authors: Syrjänen
mesh_terms: D000328:Adult; D000368:Aged; D002681:China / Q000453:epidemiology; D019411:Clinical Laboratory Techniques; D004279:DNA, Viral / Q000302:isolation & purification; D004938:Esophageal Neoplasms / Q000453:epidemiology / Q000821:virology*; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D012307:Risk Factors; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral