Antibody-mediated inhibition of the human alloimmune response to platelet transfusion in Hu-PBL-SCID mice.
Severe combined immune deficient (SCID) mice were engrafted with human (Hu) peripheral blood lymphocytes (PBL) from a previously alloimmunized donor and transfused with HLA-mismatched platelets. We have previously shown this to be a useful model for platelet transfusion. These engrafted mice (Hu-PBL-SCID mice) produced high levels of alloantibody in response to standard platelet preparations. However, when the first platelet challenge was presensitized with anti-HLA antibody and then transfused there was a marked reduction in the amount of alloantibody produced to five subsequent untreated platelet transfusions. Platelets pretreated with platelet-specific anti-HPA-1a (PL(A1)) sera did not induce a decrease in the anti-HLA alloantibody response. This demonstrated that platelet-induced HLA alloimmunization can be blocked by anti-HLA antibody-sensitized cells.
Crow
A R
AR
Division of Haematology/Oncology, St Michael's Hospital, Toronto, Ontario, Canada.
Freedman
J
J
Hannach
B
B
Blanchette
V
V
Lazarus
A H
AH
D000818:Animals; D000917:Antibody Formation; D015551:Autoimmunity / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology; D001792:Blood Platelets / Q000276:immunology*; D002455:Cell Division; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis / Q000276:immunology*; D051379:Mice; D016513:Mice, SCID; D017713:Platelet Transfusion; D013601:T-Lymphocytes / Q000166:cytology
D007074:Immunoglobulin G; D007518:Isoantibodies