Comparative study of techniques used to recover viruses from residual urban sludge.
Eight virus extraction techniques were compared on three types of residual urban sludge for simultaneous detection of infectious enteroviruses, somatic coliphages, F-specific RNA bacteriophages and Bacteroides fragilis bacteriophages. The highest virus counts were found in extracts obtained using three extraction techniques described by respectively using a 10% beef extract solution at pH 9 and sonication, using a 0.3 M NaCl/7% beef extract solution at pH 7.5 and freon, and finally using a 0.1 M borate buffer/3% beef extract solution at pH 9 and sonication.
Mignotte
B
B
U.M.R. University CNRS 7564, Laboratory of Virology, Faculty of Pharmacy, Nancy, France. lschwart@pharma.u-nancy.fr
Maul
A
A
Schwartzbrod
L
L
D001435:Bacteriophages / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000821:virology; D003090:Coliphages / Q000302:isolation & purification; D004770:Enterovirus / Q000302:isolation & purification*; D006801:Humans; D007220:Industrial Waste; D012317:RNA Phages / Q000302:isolation & purification; D012722:Sewage / Q000821:virology*; D010948:Viral Plaque Assay; D014871:Water Microbiology
D007220:Industrial Waste; D012722:Sewage