title: Oxytocin receptor expression in human term and preterm gestational tissues prior to and following the onset of labour.
abstract: Oxytocin receptor (OTR) mRNA expression has previously been demonstrated in human myometrium, decidua, chorion and amnion but the effect of gestational age and the onset of labour has not been determined in these individual tissues. Spatial OTR mRNA expression was examined by in situ hybridization and ligand binding was confirmed using autoradiography with the iodinated oxytocin antagonist d(CH2)5[Tyr(Me)2,Thr4,Tyr-NH29]-vasotocin (125I-OTA). Tissue was collected at term (>37 weeks of gestation) or preterm (24-36 weeks of gestation) caesarean section and classified as labour (contractions every 5 min associated with cervical dilatation) or non-labour. OTR mRNA expression was measured as optical density units from autoradiographs. There was a highly significant (P<0.001) effect of tissue type on expression of OTR mRNA with expression greatest in myometrium, low in decidua and chorion and not detected in placenta. Similar results were obtained with the 125I-OTA-binding studies, indicating that the message was translated. Amnion had an apparently high level of both hybridization and 125I-OTA binding in some samples, but a lack of specificity prevented quantification of the signal in this tissue type. Term myometrium (labour and non-labour) had significantly higher (P<0.01) OTR mRNA expression than preterm myometrium, but there was no further increase in mRNA concentration associated with labour onset. In contrast, 125I-OTA binding in myometrium was already high at 33 weeks and did not increase further either later in pregnancy or with labour. In decidua there was no effect of gestational age or labour onset on OTR mRNA expression or 125I-OTA binding. In summary, OTR mRNA expression in the myometrium increased in late pregnancy whereas decidual expression was much lower and did not rise at term.
authors: The Royal Veterinary College, Reproduction and Development Group, Boltons Park, Hawkshead Road, Potters Bar, Hertfordshire, EN6 1NB, UK.
authors: D C
authors: DC
authors: Wathes
authors: S C
authors: SC
authors: Borwick
authors: P M
authors: PM
authors: Timmons
authors: S T
authors: ST
authors: Leung
authors: S
authors: S
authors: Thornton
mesh_terms: D000650:Amnion / Q000378:metabolism; D001345:Autoradiography; D002823:Chorion / Q000378:metabolism; D003656:Decidua / Q000378:metabolism; D005260:Female; D005865:Gestational Age; D006801:Humans; D017403:In Situ Hybridization; D007745:Labor Onset; D007743:Labor, Obstetric / Q000378:metabolism*; D009215:Myometrium / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D011869:Radioligand Assay; D018045:Receptors, Oxytocin / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D018045:Receptors, Oxytocin