Comparison of recombinant and purified human bone morphogenetic protein.
Clinically, it would be more convenient to use recombinant than purified preparations of bone morphogenetic protein (BMP). Recently, recombinant human BMP (rhBMP) has attracted the attention of many investigators, but it has not been fully characterized. We examined the bone-inducing activity of rhBMP-2 and compared it with that of purified BMP derived from human bone matrix (phBMP). Two, 10, or 50 microg of rhBMP-2 or phBMP was mixed with 3 mg of atelopeptide type I collagen (carrier), and specimens were implanted in the calf muscles of Wistar rats (n=5 in each group). Four weeks later, new bone had formed in all the rhBMP-2- and phBMP-implanted muscles and was visible radiographically and histologically. The quantitative analysis indicated that the activity of rhBMP-2 was less than one tenth that of phBMP. It is necessary to find out why rhBMP-2 has fewer activities than phBMP.
Bessho
K
K
Department of Oral and Maxillofacial Surgery, Faculty of Medicine, Kyoto University, Japan.
Kusumoto
K
K
Fujimura
K
K
Konishi
Y
Y
Ogawa
Y
Y
Tani
Y
Y
Iizuka
T
T
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000473:pathology; D001857:Bone Matrix / Q000187:drug effects / Q000473:pathology; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002118:Calcium / Q000032:analysis; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000187:drug effects / Q000473:pathology; D018482:Muscle, Skeletal / Q000737:chemistry / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects*; D011506:Proteins / Q000032:analysis; D011859:Radiography; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
C525718:BMP2 protein, human; C525720:Bmp2 protein, rat; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D011506:Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000469:Alkaline Phosphatase; D002118:Calcium