Catecholamines in pericardial fluid of normotensive, spontaneously hypertensive and reserpine-treated rats.
In this study our aims were to investigate the presence and source of catecholamines in pericardial fluid of normotensive, reserpine-treated and spontaneously hypertensive rats. We found that noradrenaline is the only detectable catecholamine present in rat pericardial fluid. The effect of reserpine 6, 12, and 214 h after pre-treatment with 5 mg kg(-1) (8.2 micromol kg(-1)) i.p. shows that the concentration of noradrenaline in pericardial fluid reflects the amount of noradrenaline released within the heart rather than the amount of noradrenaline in plasma. Using spontaneously hypertensive rats (SHR) as a model for primary hypertension we could show that the level of pericardial noradrenaline is approximately threefold in the pericardial fluid of the SHRs when compared to respective values of age-matched normotensive Wistar-Kyoto rats (WKY), suggesting that there was an increased noradrenaline overflow in the hearts of the SHRs. In conclusion, determination of the noradrenaline concentration in the pericardial fluid might provide a new method for estimating the release of noradrenaline in the heart.
Klemola
R
R
Department of Physiology, University of Oulu, Finland.
Huttunen
P
P
Laine
M
M
Weckström
M
M
Hirvonen
J
J
D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D006321:Heart / Q000503:physiopathology; D006973:Hypertension / Q000188:drug therapy* / Q000378:metabolism* / Q000503:physiopathology; D008297:Male; D009638:Norepinephrine / Q000097:blood / Q000378:metabolism*; D010490:Pericardial Effusion / Q000378:metabolism* / Q000503:physiopathology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D017207:Rats, Sprague-Dawley; D012110:Reserpine / Q000494:pharmacology*
D000959:Antihypertensive Agents; D012110:Reserpine; D009638:Norepinephrine