Design and synthesis of a potent cyclic analogue of the myelin basic protein epitope MBP72-85: importance of the Ala81 carboxyl group and of a cyclic conformation for induction of experimental allergic encephalomyelitis.
Experimental allergic encephalomyelitis (EAE) is induced in susceptible animals by immunodominant determinants of myelin basic protein (MBP), such as guinea pig sequence MBP72-85. Two linear and one cyclic analogues based on MBP72-85 have been synthesized and evaluated for EAE induction in Lewis rats. The linear peptide Gln1-Lys2-Ser3-Gln4-Arg5-Ser6-Gln7-+ ++Asp8-Glu9-Asn10-Pro11-Val12 (1) was found to induce EAE, while substitution of the Asp residue at position 8 with Ala resulted in an analogue (2) which suppressed the induction of EAE by its parent peptide. Nuclear magnetic resonance studies of analogue 1 in dimethyl sulfoxide (DMSO) using TOCSY/ROESY techniques revealed a head-to-tail intramolecular interaction (ROE connectivity between betaVal12-gammaGln1), indicating a pseudocyclic conformation for the immunogenic peptide 1. A conformational model was developed using NMR constraints and molecular dynamics. Based on this model, a novel amide-linked cyclic analogue has been designed and synthesized by connecting the side-chain amino and carboxyl groups of Lys and Glu at positions 2 and 9, respectively, of linear analogue 1. The cyclic analogue (3) had similar activity to the linear peptide 1, and the EAE effects induced by cyclic analogue 3 were completely suppressed by co-injection with the Ala81-substituted analogue 2 in Lewis rats. The similar potencies of analogues 1 and 3 support the proposed cyclic comformation suggested for analogue 1 from NMR studies and computer modeling and provides the basis for designing more potent molecules with improved properties such as increased resistance to degradation.15 The present findings suggest that a cyclic conformation for the MBP72-85 epitope positions the carboxyl group of Asp81 correctly and presumably other side groups of the peptide such as Arg78 in a manner which enables functional binding of the trimolecular complex MHC-peptide-T cell receptor resulting in EAE.
Tselios
T
T
Department of Chemistry, University of Patras, 26500 Patras, Greece.
Probert
L
L
Daliani
I
I
Matsoukas
E
E
Troganis
A
A
Gerothanassis
I P
IP
Mavromoustakos
T
T
Moore
G J
GJ
Matsoukas
J M
JM
D000409:Alanine / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D015195:Drug Design; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D000939:Epitopes; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D004676:Myelin Basic Protein / Q000037:antagonists & inhibitors / Q000138:chemical synthesis* / Q000737:chemistry* / Q000276:immunology; D010446:Peptide Fragments / Q000037:antagonists & inhibitors / Q000138:chemical synthesis* / Q000737:chemistry* / Q000276:immunology; D010456:Peptides, Cyclic / Q000037:antagonists & inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D051381:Rats; D011917:Rats, Inbred Lew; D013329:Structure-Activity Relationship
D000939:Epitopes; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D010456:Peptides, Cyclic; C118872:myelin basic protein 72-85; D000409:Alanine