Immunization with potato plants expressing VP60 protein protects against rabbit hemorrhagic disease virus.
The major structural protein VP60 of rabbit hemorrhagic disease virus (RHDV) has been produced in transgenic potato plants under the control of a cauliflower mosaic virus 35S promoter or a modified 35S promoter that included two copies of a strong transcriptional enhancer. Both types of promoters allowed the production of specific mRNAs and detectable levels of recombinant VP60, which were higher for the constructs carrying the modified 35S promoter. Rabbits immunized with leaf extracts from plants carrying this modified 35S promoter showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease.
Castañón
S
S
Departamento de Biología de Organismos y Sistemas, Instituto Universitario de Biotecnología de Asturias (CSIC), Universidad de Oviedo, 33006 Oviedo, Spain.
Marín
M S
MS
Martín-Alonso
J M
JM
Boga
J A
JA
Casais
R
R
Humara
J M
JM
Ordás
R J
RJ
Parra
F
F
D000818:Animals; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D017250:Caliciviridae Infections / Q000276:immunology / Q000517:prevention & control*; D002213:Capsid / Q000235:genetics / Q000276:immunology*; D017929:Hemorrhagic Disease Virus, Rabbit / Q000235:genetics / Q000276:immunology*; D030821:Plants, Genetically Modified; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D011198:Solanum tuberosum; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*
D000956:Antigens, Viral; D011993:Recombinant Fusion Proteins; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus