Serotonergic innervation of the foot of the pond snail Lymnaea stagnalis (L.).
The aminergic innervation of the foot of Lymnaea stagnalis was investigated using electron microscopy, immunocytochemistry, and HPLC. The foot was found to contain large amounts of serotonin and dopamine, though at lower concentrations than are found in nervous tissue. Serotonin containing tissue was concentrated in the ventral surface of the foot, under ciliated areas of the epidermis where it occurred in varicosities, with fine tracts joining these varicosities. Varicosities also occurred in deeper tissues, probably adjacent to mucus cells. Positive fluorescence for serotonin in axons was found in nerves innervating the foot, but few neuronal cell bodies containing serotonin were detected, indicating that most of the innervation was coming from the central ganglia. Axon varicosities were found using TEM on ciliated cells, mucus cells, and muscle cells as well as interaxonal junctions (possibly non-synaptic) within nerves. The neuronal varicosities contacting the ciliated cells and mucus cells contained mostly dense-cored vesicles of between 60 and 100 nm in diameter. Smaller, lucent vesicles also occurred in these terminals. The origin and significance of this innervation is discussed. It is suggested that both serotonin and dopamine may play a large role in controlling ciliary gliding by the foot.
McKenzie
J D
JD
Department of Physiology, University of Leeds, UK.
Caunce
M
M
Hetherington
M S
MS
Winlow
W
W
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000648:ultrastructure; D015306:Biogenic Monoamines / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D002923:Cilia / Q000648:ultrastructure; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D005121:Extremities / Q000033:anatomy & histology* / Q000294:innervation*; D005455:Fluorescent Antibody Technique; D008195:Lymnaea / Q000033:anatomy & histology* / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D009132:Muscles / Q000294:innervation / Q000378:metabolism / Q000648:ultrastructure; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D012701:Serotonin / Q000378:metabolism / Q000502:physiology*
D015306:Biogenic Monoamines; D012701:Serotonin