[A syphilis marker study of blood component donors].
Laboratory testing of donor blood is the key point in prevention of hemotransmissive syphilis. The probability of using diagnostic EIA kit RecombiBest antipallidum-strip (AO Vektor-Best, Novosibirsk) is assessed. Ninety-two donor sera were tested. For assessing the sensitivity of the test system, 11 sera of syphilis patients were titrated by double dilutions 1:10 to 1:1280 for each strip. In parallel with this, antibodies to T. pallidum were assessed in the precipitation microtest. EIA proved to be not only highly specific, but more sensitive than precipitation microtest. Antibodies to T. pallidum are detected by EIA in serum dilutions at least two times higher than the minimal titers in the precipitation test. Thus, the above diagnostic kit is recommended for screening of donors of blood and its components.
Zhiburt
E B
EB
Bel'gesov
N V
NV
Bondarchuk
Iu V
IuV
Serebrianaia
N B
NB
Sidorkevich
S V
SV
D000907:Antibodies, Bacterial / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001782:Blood Donors; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000706:statistics & numerical data; D011933:Reagent Kits, Diagnostic / Q000706:statistics & numerical data; D012680:Sensitivity and Specificity; D013588:Syphilis Serodiagnosis / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D014210:Treponema pallidum / Q000276:immunology
D000907:Antibodies, Bacterial; D015415:Biomarkers; D011933:Reagent Kits, Diagnostic