Toxicity of gamma irradiated liposomes. 2. In vitro effects on cells in culture.
In this study, the effects of liposome composition and gamma irradiation on their interactions with cell cultures were studied. The cytotoxicity test and the growth inhibition test clearly revealed toxic effects of liposomes composed of unsaturated phospholipids and gamma irradiation of these preparations enhanced their toxic effects. The murine fibroblast cell-line L 929 was less affected compared to the macrophage cell-line RAW 264 with a higher endocytic capacity. On the other hand, both gamma irradiated and non-irradiated liposomes composed of saturated phospholipids were non-toxic for the cells and irradiation did not affect their drug delivery properties. Hence, it seems that gamma irradiation is appropriate for sterilisation of these liposomes.
Stensrud
G
G
School of Pharmacy, Department of Pharmaceutics, University of Oslo, Blindern, Norway. gry.stensrud@farmasi.uio.no
Mönkkönen
J
J
Karlsen
J
J
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects*; D005720:Gamma Rays / Q000009:adverse effects*; D008081:Liposomes / Q000633:toxicity*; D051379:Mice
D008081:Liposomes