Type X collagen in the human invertebral disc: an indication of repair or remodelling?
The short-chained type X collagen was once thought to be produced exclusively by hypertrophic chondrocytes during endochondral ossification. More recently, however, it has been found elsewhere, for example in articular cartilage. In the present study, the occurrence of type X collagen in the intervertebral disc has been investigated. Human disc tissues of varying pathologies were examined for the presence of type X collagen and expression of alpha1(X) mRNA by immunohistochemistry and in situ hybridization respectively. All samples of disc contained areas that were immunoreactive but to varying extents. In the disc itself, staining for the protein and alpha1(X) mRNA was seen frequently associated with cells of the nucleus pulposus, which were large and of hypertrophic appearance, most commonly found in degenerate discs, and also in areas of disorganized architecture, such as clefts. In addition, type X collagen, both protein and mRNA, was found in regions of the cartilage end-plate, which calcify ectopically in scoliotic patients. We suggest that type X collagen production may be a response of disc tissue cells to a stimulus, such as altered loading.
Roberts
S
S
Centre for Spinal Studies, Robert Jones and Agnes Hunt Orthopaedic Hospital NHS Trust, Oswestry, Shropshire, UK.
Bains
M A
MA
Kwan
A
A
Menage
J
J
Eisenstein
S M
SM
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002645:Chickens; D002648:Child; D003094:Collagen / Q000032:analysis*; D005260:Female; D006801:Humans; D007403:Intervertebral Disc / Q000737:chemistry* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D011817:Rabbits
D003094:Collagen