ICAM-3 and E-selectin endothelial cell expression differentiate two phases of angiogenesis in infantile hemangiomas.
Cellular adhesion molecules are newly identified mediators of angiogenesis. Infantile hemangiomas, characterized in the early stages by a proliferation of poorly differentiated vessels followed in the late stages by a vascular differentiation and regression of the tumor, represent an interesting model to study angiogenesis. We studied by immunohistochemistry the distribution of HLA-DR and three adhesion molecules ICAM-3, E-selectin and VCAM-1 on endothelial cells in different stages of vessel differentiation in infantile hemangiomas. We found high levels of ICAM-3 expression on proliferating vessels, while its expression was low or undetectable on well differentiated vessels. A different set of E-selectin antibodies showed a more heterogenous pattern of distribution and VCAM-1 antigens were found in both proliferating and differentiated vessels. HLA-DR expression on endothelial cells was inversely correlated to the vascular differentiation. Our results are consistent with the hypothesis that ICAM-3 plays a role in the early stages of vessel formation. Our results also suggest that variation of E-selectin and HLA-DR expression may be related either to vessel differentiation or may reflect the acquisition of an activated endothelial cell status.
Verkarre
V
V
Service d'Anatomie et de Cytologie Pathologiques, Hôpital Necker-Enfants Malades and Université René Descartes-Paris V EA 219, France.
Patey-Mariaud de Serre
N
N
Vazeux
R
R
Teillac-Hamel
D
D
Chretien-Marquet
B
B
Le Bihan
C
C
Leborgne
M
M
Fraitag
S
S
Brousse
N
N
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D019040:E-Selectin / Q000096:biosynthesis*; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D006684:HLA-DR Antigens / Q000096:biosynthesis; D018324:Hemangioma, Capillary / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D009389:Neovascularization, Pathologic; D012867:Skin / Q000098:blood supply / Q000737:chemistry / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D019010:Vascular Cell Adhesion Molecule-1 / Q000096:biosynthesis
D015703:Antigens, CD; D000943:Antigens, Differentiation; D015815:Cell Adhesion Molecules; D019040:E-Selectin; D006684:HLA-DR Antigens; C483616:ICAM3 protein, human; D019010:Vascular Cell Adhesion Molecule-1