title: Reactions of nitric oxide with the reduced non-heme diiron center of the soluble methane monooxygenase hydroxylase.
abstract: The soluble methane monooxygenase system from Methylococcus capsulatus (Bath) catalyzes the oxidation of methane to methanol and water utilizing dioxygen at a non-heme, carboxylate-bridged diiron center housed in the hydroxylase (H) component. To probe the nature of the reductive activation of dioxygen in this system, reactions of an analogous molecule, nitric oxide, with the diiron(II) form of the enzyme (Hred) were investigated by both continuous and discontinuous kinetics methodologies using optical, EPR, and Mössbauer spectroscopy. Reaction of NO with Hred affords a dinitrosyl species, designated Hdinitrosyl, with optical spectra (lambdamax = 450 and 620 nm) and Mössbauer parameters (delta = 0.72 mm/s, DeltaEQ = 1.55 mm/s) similar to those of synthetic dinitrosyl analogues and of the dinitrosyl adduct of the reduced ribonucleotide reductase R2 (RNR-R2) protein. The Hdinitrosyl species models features of the Hperoxo intermediate formed in the analogous dioxygen reaction. In the presence of protein B, Hdinitrosyl builds up with approximately the same rate constant as Hperoxo ( approximately 26 s-1) at 4 degrees C. In the absence of protein B, the kinetics of Hdinitrosyl formation were best fit with a biphasic A --> B --> C model, indicating the presence of an intermediate species between Hred and Hdinitrosyl. This result contrasts with the reaction of Hred with dioxygen, in which the Hperoxo intermediate forms in measurable quantities only in the presence of protein B. These findings suggest that protein B may alter the positioning but not the availability of coordination sites on iron for exogenous ligand binding and reactivity.
authors: Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA.
authors: D E
authors: DE
authors: Coufal
authors: P
authors: P
authors: Tavares
authors: A S
authors: AS
authors: Pereira
authors: B H
authors: BH
authors: Hyunh
authors: S J
authors: SJ
authors: Lippard
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D005296:Ferrous Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D006460:Hemolysin Proteins; D008772:Methylococcaceae / Q000201:enzymology*; D008958:Models, Molecular; D009569:Nitric Oxide / Q000737:chemistry*; D019159:Nonheme Iron Proteins / Q000138:chemical synthesis / Q000737:chemistry; D010084:Oxidation-Reduction; D010105:Oxygenases / Q000138:chemical synthesis / Q000737:chemistry*; D012264:Ribonucleotide Reductases / Q000737:chemistry; D012995:Solubility; D013053:Spectrophotometry; D015204:Spectroscopy, Mossbauer
chemical_list: D001426:Bacterial Proteins; C039092:CAMP protein, Streptococcus; D005296:Ferrous Compounds; D006460:Hemolysin Proteins; D019159:Nonheme Iron Proteins; D009569:Nitric Oxide; D010105:Oxygenases; C021555:methane monooxygenase; D012264:Ribonucleotide Reductases; C108427:ribonucleotide reductase R2 subunit