Human African trypanosomiasis: a latex agglutination field test for quantifying IgM in cerebrospinal fluid.
LATEX/IgM, a rapid agglutination test for the semi-quantitative detection of IgM in cerebrospinal fluid of patients with African trypanosomiasis, is described in this article. The lyophilized reagent has been designed for field use and remains stable at 45 degrees C for one year. The test has been evaluated on cerebrospinal fluid samples from trypanosome-infected and non-infected patients, by comparison with commercial latex agglutination, radial immunodiffusion, and nephelometry. All test systems yielded similar results.
Lejon
V
V
Department of Parasitology, University of Antwerp, Belgium.
Büscher
P
P
Sema
N H
NH
Magnus
E
E
Van Meirvenne
N
N
D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000134:cerebrospinal fluid*; D007202:Indicators and Reagents; D007841:Latex Fixation Tests; D009391:Nephelometry and Turbidimetry; D014353:Trypanosomiasis, African / Q000134:cerebrospinal fluid / Q000175:diagnosis*
D007075:Immunoglobulin M; D007202:Indicators and Reagents