Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea.
Identification of common dietary substances capable of affording protection or modulating the onset and severity of arthritis may have important human health implications. An antioxidant-rich polyphenolic fraction isolated from green tea (green tea polyphenols, GTPs) has been shown to possess anti-inflammatory and anticarcinogenic properties in experimental animals. In this study we determined the effect of oral consumption of GTP on collagen-induced arthritis in mice. In three independent experiments mice given GTP in water exhibited significantly reduced incidence of arthritis (33% to 50%) as compared with mice not given GTP in water (84% to 100%). The arthritis index also was significantly lower in GTP-fed animals. Western blot analysis showed a marked reduction in the expression of inflammatory mediators such as cyclooxygenase 2, IFN-gamma, and tumor necrosis factor alpha in arthritic joints of GTP-fed mice. Histologic and immunohistochemical analysis of the arthritic joints in GTP-fed mice demonstrated only marginal joint infiltration by IFN-gamma and tumor necrosis factor alpha-producing cells as opposed to massive cellular infiltration and fully developed pannus in arthritic joints of non-GTP-fed mice. The neutral endopeptidase activity was approximately 7-fold higher in arthritic joints of non-GTP-fed mice in comparison to nonarthritic joints of unimmunized mice whereas it was only 2-fold higher in the arthritic joints of GTP-fed mice. Additionally, total IgG and type II collagen-specific IgG levels were lower in serum and arthritic joints of GTP-fed mice. Taken together our studies suggest that a polyphenolic fraction from green tea that is rich in antioxidants may be useful in the prevention of onset and severity of arthritis.
Haqqi
T M
TM
Department of Medicine, Division of Rheumatic Diseases, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Anthony
D D
DD
Gupta
S
S
Ahmad
N
N
Lee
M S
MS
Kumar
G K
GK
Mukhtar
H
H
D000818:Animals; D000917:Antibody Formation; D001169:Arthritis, Experimental / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D002645:Chickens; D003094:Collagen / Q000276:immunology; D005419:Flavonoids; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007371:Interferon-gamma / Q000032:analysis / Q000235:genetics; D007596:Joints / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010636:Phenols / Q000302:isolation & purification / Q000627:therapeutic use*; D011108:Polymers / Q000302:isolation & purification / Q000627:therapeutic use*; D059808:Polyphenols; D013662:Tea; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics
D005419:Flavonoids; D007074:Immunoglobulin G; D010636:Phenols; D011108:Polymers; D059808:Polyphenols; D013662:Tea; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D003094:Collagen