Addition of fresh blood to intrastriatal grafts of embryonic mesencephalon into the hemiparkinsonian rat does not impair the survival of grafted dopaminergic neurones.
Cell transplantation therapy for Parkinson's patients, although seen to bring benefit to some patients during first clinical trials, remains impracticable on a large scale, in part because of the poor survival of the dopaminergic neurones transplanted. The loss of dopaminergic neurones occurs rapidly over the first 1-2 days after transplantation, in response to factors intrinsic to the host brain. Here we investigated whether contamination of the grafted cell suspension with blood during the transplantation procedure may be one factor responsible for the poor survival of DA neurons within the graft, possibly through factors such as free iron or complement. 6-Hydroxydopamine lesioned rats were grafted with 2 microl suspension of dissociated E14 ventral mesencephalon to which 1 microl blood or 1 microl grafting medium was added. After 6 weeks, there was no significant difference in the number of surviving DA neurones in the two groups. We conclude that contamination of grafts with blood is not a major factor responsible for the extensive death of dopaminergic neurones within them.
Zietlow
R
R
MRC Cambridge Centre for Brain Repair, University of Cambridge, Forvie Site, Cambridge, CB2 2PY, United Kingdom.
Dunnett
S B
SB
Fawcett
J W
JW
D000818:Animals; D001769:Blood; D017690:Cell Transplantation / Q000009:adverse effects; D004298:Dopamine / Q000378:metabolism*; D005260:Female; D006085:Graft Survival / Q000502:physiology*; D017072:Neostriatum / Q000201:enzymology / Q000473:pathology* / Q000601:surgery; D009474:Neurons / Q000378:metabolism / Q000473:pathology*; D010302:Parkinson Disease, Secondary / Q000201:enzymology / Q000473:pathology* / Q000601:surgery; D051381:Rats; D013378:Substantia Nigra / Q000166:cytology* / Q000196:embryology / Q000201:enzymology; D014184:Transplantation, Homologous; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine