title: Ovalbumin in developing chicken eggs migrates from egg white to embryonic organs while changing its conformation and thermal stability.
abstract: Ovalbumin was detected in developing chicken eggs. The large majority of these ovalbumin molecules was found to be in a heat-stable form reminiscent of S-ovalbumin. About 83 and 90% of the ovalbumin population was in a heat-stable form in day 14 or stage 40 amniotic fluid and day 18 or stage 44 egg yolk, respectively, whereas ovalbumin in newly deposited eggs was in the heat-unstable, native form. Purified preparations of stable ovalbumin from egg white and amniotic fluid showed a less ordered configuration than native ovalbumin, as analyzed by circular dichroism and differential scanning calorimetry. In addition, mass spectrometric analysis exhibited distinct size microheterogeneity between the stable and native forms of ovalbumin. Immunohisotochemical study revealed that ovalbumin was present in the central nervous system and other embryonic organs. These results indicated that egg white ovalbumin migrates into the developing embryo while changing its higher order structure.
authors: Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. yasushi@chem.agr.kagoshima-u.ac.jp
authors: Y
authors: Y
authors: Sugimoto
authors: S
authors: S
authors: Sanuki
authors: S
authors: S
authors: Ohsako
authors: Y
authors: Y
authors: Higashimoto
authors: M
authors: M
authors: Kondo
authors: J
authors: J
authors: Kurawaki
authors: H R
authors: HR
authors: Ibrahim
authors: T
authors: T
authors: Aoki
authors: T
authors: T
authors: Kusakabe
authors: K
authors: K
authors: Koga
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002152:Calorimetry, Differential Scanning; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D002942:Circular Dichroism; D017931:DNA Primers; D004529:Egg White; D004591:Electrophoresis, Polyacrylamide Gel; D004625:Embryo, Nonmammalian; D006358:Hot Temperature; D010047:Ovalbumin / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D010047:Ovalbumin