Proteolytic destruction of functional proteins by phagocytes in human peritonitis.
BACKGROUND
Besides phagocyte-derived oxidative autoaggression, proteolytic destruction of functional proteins in the peritoneal cavity may also be involved in the pathomechanism of secondary peritonitis. To evaluate the pattern of proteolysis, 43 patients undergoing initial operation for acute peritonitis (n = 30) or scheduled abdominal lavages (Etappenlavage) for resolution of persistent peritonitis (n = 13) and 16 surgical patients with abdominal exudation without peritonitis were enrolled in our study.


METHODS
Thirty blood samples and purulent exudates were taken simultaneously in each peritonitis group at the surgical interventions. Sixteen clear exudates were obtained from patients with post-operative non-infectious irritations. The following parameters were measured: (a) elastase (from neutrophils) and cathepsin B (from monocytes/macrophages); (b) alpha1-proteinase inhibitor (alpha1PI) and overall cysteine proteinase inhibitor capacity; and (c) opsonic activity and degradation products of fibrinogen, complement C3 and immunoglobulin IgG.


RESULTS
Circulating levels of phagocyte proteinases and of alpha1PI were significantly elevated, whereas antigen concentrations and opsonic activity of C3 and IgG were slightly reduced in peritonitis patients compared to healthy volunteers. No degradation products were detectable in patients' blood. Discharge of phagocyte proteinases was even more pronounced in both types of peritonitis exudates. Although most of the elastase was complexed with alpha1PI, active elastase and its specific fibrinogen split product was found along with significantly reduced inhibitory capacity for elastase and cysteine proteinases. Local opsonic activity was dramatically diminished because of proteolytic degradation of C3 and IgG. Despite some phagocyte proteinase release, no destruction of functional proteins was seen in clear exudates.


CONCLUSIONS
Higher values of extracellularly released phagocyte proteinases concomitant with lower opsonin activity in exudates from patients with persistent peritonitis can be taken as a further hint of the involvement of local proteolysis-induced pathomechanisms in the development of lethal multiple organ failure, which occurred more frequently in patients with persistent peritonitis (54%) than in those with acute peritonitis (27%).
Jochum
M
M
Klinikum Innenstadt der Universität München, Munich, Germany. jochum@clinbio.med.uni-muenchen.de
Billing
A G
AG
Fröhlich
D
D
Schildberg
F W
FW
MacHleidt
W
W
Cheronis
J C
JC
Gippner-Steppert
C
C
Fritz
H
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002199:Capillary Permeability; D002401:Cathepsin B / Q000502:physiology; D003176:Complement C3 / Q000378:metabolism; D010450:Endopeptidases / Q000502:physiology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology; D008297:Male; D008875:Middle Aged; D010196:Pancreatic Elastase / Q000502:physiology; D010538:Peritonitis / Q000378:metabolism*; D010586:Phagocytes / Q000378:metabolism*
D003176:Complement C3; D007074:Immunoglobulin G; D010450:Endopeptidases; D010196:Pancreatic Elastase; D002401:Cathepsin B