title: The crystal structure of a 2-fluorocellotriosyl complex of the Streptomyces lividans endoglucanase CelB2 at 1.2 A resolution.
abstract: Glycoside hydrolases have been classified into over 66 families on the basis of amino acid sequence. Recently a number of these families have been grouped into "clans" which share a common fold and catalytic mechanism [Henrissat, B., and Bairoch, A. (1996) Biochem. J. 316, 695-696]. Glycoside hydrolase Clan GH-C groups family 11 xylanases and family 12 cellulases, which share the same jellyroll topology, with two predominantly antiparallel beta-sheets forming a long substrate-binding cleft, and act with net retention of anomeric configuration. Here we present the three-dimensional structure of a family 12 endoglucanase, Streptomyces lividans CelB2, in complex with a 2-deoxy-2-fluorocellotrioside. Atomic resolution (1.2 A) data allow clear identification of two distinct species in the crystal. One is the glycosyl-enzyme intermediate, with the mechanism-based inhibitor covalently linked to the nucleophile Glu 120, and the other a complex with the reaction product, 2-deoxy-2-fluoro-beta-D-cellotriose. The active site architecture of the complex provides insight into the double-displacement mechanism of retaining glycoside hydrolases and also sheds light on the basis of the differences in specificity between family 12 cellulases and family 11 xylanases.
authors: Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York, Y010 5DD, U.K.
authors: G
authors: G
authors: Sulzenbacher
authors: L F
authors: LF
authors: Mackenzie
authors: K S
authors: KS
authors: Wilson
authors: S G
authors: SG
authors: Withers
authors: C
authors: C
authors: Dupont
authors: G J
authors: GJ
authors: Davies
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D018360:Crystallography, X-Ray; D008958:Models, Molecular; D009844:Oligosaccharides / Q000737:chemistry*; D013302:Streptomyces / Q000201:enzymology*
chemical_list: C118765:2-deoxy-2-fluorocellotriose; D009844:Oligosaccharides; D002480:Cellulase