Molecular cloning and sequencing of the cDNA for vacuolar H+-pyrophosphatase from Chara corallina1.
We have cloned a cDNA for vacuolar proton-translocating pyrophosphatase of Chara corallina that is one of the closest green algae to the land plants. The deduced protein consists of 793 amino acid residues. Its sequence is 71% identical to the H+-pyrophosphatases of land plants, and is less than 46% identical to those of marine alga and phototrophic bacterium.
Nakanishi
Y
Y
Laboratory of Biochemistry, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Matsuda
N
N
Aizawa
K
K
Kashiyama
T
T
Yamamoto
K
K
Mimura
T
T
Ikeda
M
M
Maeshima
M
M
D000595:Amino Acid Sequence; D001483:Base Sequence; D000460:Chlorophyta / Q000201:enzymology / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D018076:DNA, Complementary / Q000096:biosynthesis / Q000737:chemistry; D043564:Inorganic Pyrophosphatase; D008969:Molecular Sequence Data; D011755:Pyrophosphatases / Q000096:biosynthesis / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D011755:Pyrophosphatases; D043564:Inorganic Pyrophosphatase