title: EB/RP gene family encodes tubulin binding proteins.
abstract: Mutations in the adenomatous polyposis coli (APC) gene are linked to the dysplastic transformation of colorectal polyps and represent an early step in the development of colorectal tumors. Ninety-four percent of all mutations result in the expression of a truncated APC protein lacking the C-terminal region. The C-terminal region of the APC protein may have a tumor suppressor function as its absence appears to be linked to the development of dysplastic lesions. Recently, we discovered and characterized a protein called RP1 which binds specifically to the C-terminal region of the APC protein. We show now that RP1 and the other known members of the EB/RP family (EB1 and RP3) also bind directly to tubulin, both in vitro and in vivo. Immunohistochemical analyses reveal a distinct staining pattern during interphase as well as an association of RP1/EB1 with mitotic microtubule structures. The previously described puncta of the APC protein at the leading edge of membrane protrusions contact microtubule fibers that contain RP1 or EB1.
authors: Medical Department I, Saarland University, Homburg, Germany.
authors: J P
authors: JP
authors: Juwana
authors: P
authors: P
authors: Henderikx
authors: A
authors: A
authors: Mischo
authors: A
authors: A
authors: Wadle
authors: N
authors: N
authors: Fadle
authors: K
authors: K
authors: Gerlach
authors: J W
authors: JW
authors: Arends
authors: H
authors: H
authors: Hoogenboom
authors: M
authors: M
authors: Pfreundschuh
authors: C
authors: C
authors: Renner
mesh_terms: D011125:Adenomatous Polyposis Coli / Q000235:genetics; D000595:Amino Acid Sequence; D002352:Carrier Proteins / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005136:Eye Proteins; D005815:Genetic Code; D006801:Humans; D007150:Immunohistochemistry; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D008938:Mitosis / Q000502:physiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D011506:Proteins / Q000235:genetics*; D017386:Sequence Homology, Amino Acid; D013347:Subcellular Fractions / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C405804:EB1 microtubule binding proteins; D005136:Eye Proteins; D008869:Microtubule-Associated Proteins; D011506:Proteins; C099929:RPGR protein, human; D014404:Tubulin