title: Type 1 CD4(+) T-cell help is required for induction of antipeptide multispecific cytotoxic T lymphocytes by a lipopeptidic vaccine in rhesus macaques.
abstract: We have optimized the induction of antiviral cytotoxic T lymphocytes (CTL) in rhesus macaques by a lipopeptide vaccine containing seven peptides from simian immunodeficiency virus (SIV) Nef and Gag proteins and a strong T-helper peptide from tetanus toxoid (TT) that is promiscuous in humans (peptide TT 830-846). Two of the eight immunized macaques showed T-helper (Th) cell proliferation and a specific synthesis of gamma interferon in response to TT 830-846 peptide. They also showed multispecific cytotoxic activity against three to five of the immunizing SIV peptides. These results show the importance of a strong specific type 1 Th response for inducing a multispecific CTL response in vivo, which is essential for the development of an anti-human immunodeficiency virus vaccine.
authors: Institut Cochin de Génétique Moléculaire (ICGM), Laboratoire d'Immunologie des Pathologies Infectieuses et Tumorales, INSERM U445-Université René Descartes, Hôpital Cochin, 75014 Paris, France.mortara@cochin.inser.fr
authors: L
authors: L
authors: Mortara
authors: H
authors: H
authors: Gras-Masse
authors: C
authors: C
authors: Rommens
authors: A
authors: A
authors: Venet
authors: J G
authors: JG
authors: Guillet
authors: I
authors: I
authors: Bourgault-Villada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015683:Gene Products, gag / Q000276:immunology*; D015693:Gene Products, nef / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D008074:Lipoproteins / Q000276:immunology*; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology; D018449:SAIDS Vaccines / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D013745:Tetanus Toxoid / Q000276:immunology; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D015683:Gene Products, gag; D015693:Gene Products, nef; D000949:Histocompatibility Antigens Class II; D008074:Lipoproteins; D010455:Peptides; D018449:SAIDS Vaccines; D013745:Tetanus Toxoid; D014614:Vaccines, Synthetic; D007371:Interferon-gamma