Effect of applying lactic acid bacteria isolated from forage crops on fermentation characteristics and aerobic deterioration of silage.
Two selected strains, Lactobacillus casei FG 1 and Lactobacillus plantarum FG 10 that were isolated from forage crops were used as additives at 1.0 x 10(5) cfu/g of fresh matter to alfalfa, Italian ryegrass, and sorghum, and their effect on fermentation characteristics and aerobic deterioration of silage was studied. The three silages treated with strains FG 1 or FG 10 were well preserved; had significantly lower pH values, butyric acid, propionic acid, and ammonia N concentrations, gas production, and dry matter losses; and had significantly higher contents of residual water-soluble carbohydrates and lactic acid than did the respective control silages. Yeast counts were high in all treated silages and increased rapidly during aerobic exposure. As a result, treated silages spoiled faster upon aerobic exposure than did the respective control silages. Most yeasts isolated from deteriorated silages showed high tolerance to lactic acid but low tolerance to butyric acid, and they were able to grow at low pH conditions and assimilate lactic acid. The results confirmed that L. casei and L. plantarum improved fermentation quality but did not inhibit the growth of silage yeast or aerobic deterioration of the silage.
Cai
Y
Y
Japan Collection of Microorganisms, Institute of Physical and Chemical Research, Saitama, Japan.
Benno
Y
Y
Ogawa
M
M
Kumai
S
S
D000332:Aerobiosis; D000641:Ammonia / Q000032:analysis; D020148:Butyric Acid / Q000032:analysis; D002523:Edible Grain; D005285:Fermentation; D005519:Food Preservation; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism*; D007780:Lacticaseibacillus casei / Q000378:metabolism*; D008129:Lolium; D000455:Medicago sativa; D011422:Propionates / Q000032:analysis; D012820:Silage / Q000032:analysis / Q000382:microbiology*; D015003:Yeasts
D011422:Propionates; D020148:Butyric Acid; D019344:Lactic Acid; D000641:Ammonia; C029658:propionic acid