title: Autocrine regulation of volume-sensitive anion channels in airway epithelial cells by adenosine.
abstract: The activity of volume-sensitive Cl- channels was studied in human tracheal epithelial cells (9HTEo-) by taurine efflux experiments. The efflux elicited by a hypotonic shock was partially inhibited by adenosine receptor antagonists, by alpha,beta-methyleneadenosine 5'-diphosphate (alphabetaMeADP), an inhibitor of the 5'-ectonucleotidase, and by adenosine deaminase. On the other hand, dipyridamole, a nucleoside transporter inhibitor, increased the swelling-induced taurine efflux. Extracellular ATP and adenosine increased taurine efflux by potentiating the effect of hypotonic shock. alphabetaMeADP strongly inhibited the effect of extracellular ATP but not that of adenosine. These results suggest that anion channel activation involves the release of intracellular ATP, which is then degraded to adenosine by specific ectoenzymes. Adenosine then binds to purinergic receptors, causing the activation of the channels. To directly demonstrate ATP efflux, cells were loaded with [3H]AMP, and the release of radiolabeled molecules was analyzed by high performance liquid chromatography. During hypotonic shock, cell supernatants showed the presence of ATP, ADP, and adenosine. alphabetaMeADP inhibited adenosine formation and caused the appearance of AMP. Under hypotonic conditions, elevation of intracellular Ca2+ by ionomycin caused an increase of ATP and adenosine in the extracellular solution. Our results demonstrate that volume-sensitive anion channels are regulated with an autocrine mechanism involving swelling-induced ATP release and then hydrolysis to adenosine.
authors: Laboratory of Molecular Genetics, Gaslini Institute, 16148 Genova, Italy.
authors: L
authors: L
authors: Musante
authors: O
authors: O
authors: Zegarra-Moran
authors: P G
authors: PG
authors: Montaldo
authors: M
authors: M
authors: Ponzoni
authors: L J
authors: LJ
authors: Galietta
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000241:Adenosine / Q000494:pharmacology*; D002461:Cell Line, Transformed; D018118:Chloride Channels / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D009997:Osmotic Pressure; D014132:Trachea / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D018118:Chloride Channels; D010738:Type C Phospholipases; D000241:Adenosine