Immunization with a live, attenuated simian immunodeficiency virus vaccine leads to restriction of viral diversity in Rhesus macaques not protected from pathogenic challenge.
Rhesus macaques immunized with simian immunodeficiency virus SIVmac239Deltanef but not protected from SIVmac251 challenge were studied to determine the genetic and biological characteristics of the breakthrough viruses. Assessment of SIV genetic diversity (env V1-V2) revealed a reduction in the number of viral species in the immunized, unprotected macaques, compared to the number in nonimmunized controls. However, no evidence for selection of a specific V1-V2 genotype was observed, and biologically cloned isolates from the animals with breakthrough virus were similar with respect to replication kinetics and coreceptor use in vitro.
Sodora
D L
DL
University of Texas Southwestern Medical Center, Dallas, Texas 75235, USA.
Sheridan
K E
KE
Marx
P A
PA
Connor
R I
RI
D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D015693:Gene Products, nef / Q000235:genetics; D005838:Genotype; D006801:Humans; D008253:Macaca mulatta; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D011991:Receptors, Virus / Q000378:metabolism; D018449:SAIDS Vaccines / Q000276:immunology*; D017386:Sequence Homology, Amino Acid; D016097:Simian Acquired Immunodeficiency Syndrome / Q000517:prevention & control / Q000821:virology*; D015302:Simian Immunodeficiency Virus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000502:physiology; D014611:Vaccination; D014613:Vaccines, Attenuated / Q000276:immunology; D014779:Virus Replication
D015693:Gene Products, nef; D011991:Receptors, Virus; D018449:SAIDS Vaccines; D014613:Vaccines, Attenuated