title: An Arabidopsis mutant with deregulated ABA gene expression: implications for negative regulator function.
abstract: The physiological acclimation of plants to osmotic stresses involves a complex programme of gene regulation. In one signalling pathway, elevated levels of abscisic acid (ABA) activate a subset of stress genes. Because ABA responses lack a definable morphological phenotype, we have screened for mutants that exhibit deregulated ABA-responsive gene expression. To monitor this ABA response, a line of Arabidopsis thaliana carrying a transgene composed of the ABA-responsive Arabidopsis kin2 promoter fused to the coding sequence for the firefly luciferase gene, kin2::luc, was generated. Patterns of ABA-responsive luciferase activity were monitored by photon counting. In contrast to wild-type plants which display a transient activation of kin2::luc, an ABA deregulated gene expression mutant (ade1) exhibits both sustained and enhanced levels of transgene activity. Levels of kin2, cor47 and rab18 expression in ade1 plants are also enhanced and prolonged indicating that the molecular mechanism(s) altered in ade1 plants affects the regulation of other ABA-responsive genes. The mutant phenotype is specific for the ABA response as cold-inducible kin2 expression is unaltered in ade1 plants. Genetic analyses indicate that the ade1 mutant is a monogenic recessive trait. A role for negative regulator function in ABA signalling is discussed.
authors: Laboratory for Plant Molecular Biology, Rockefeller University, New York, NY 10021, USA.
authors: R
authors: R
authors: Foster
authors: N H
authors: NH
authors: Chua
mesh_terms: D000040:Abscisic Acid / Q000235:genetics*; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D017931:DNA Primers; D018506:Gene Expression Regulation, Plant; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D010940:Plant Proteins; C075575:KIN 2 protein, Arabidopsis; D000040:Abscisic Acid; D008156:Luciferases