Cellular elongation factor 1delta is modified in cells infected with representative alpha-, beta-, or gammaherpesviruses.
Earlier reports (Y. Kawaguchi, R. Bruni, and B. Roizman, J. Virol. 71:1019-1024, 1997; Y. Kawaguchi, C. Van Sant, and B. Roizman, J. Virol. 72:1731-1736, 1998) showed that herpes simplex virus 1 (HSV-1) infection causes the hyperphosphorylation of translation elongation factor 1delta (EF-1delta) and that the modification of EF-1delta is the consequence of direct phosphorylation by a viral protein kinase encoded by the UL13 gene of HSV-1. The UL13 gene is conserved in members of all herpesvirus subfamilies. Here we report the following. (i) In various mammalian cells, accumulation of the hyperphosphorylated form of EF-1delta is observed after infection with alpha-, beta-, and gammaherpesviruses, including HSV-2, feline herpesvirus 1, pseudorabiesvirus, bovine herpesvirus 1, human cytomegalovirus (HCMV), and equine herpesvirus 2. (ii) In human lung fibroblast cells infected with recombinant HSV-1 lacking the UL13 gene, the hypophosphorylated form of EF-1delta is a minor species, whereas the amount of the hyperphosphorylated form of EF-1delta significantly increases in cells infected with the recombinant HSV-1 in which UL13 had been replaced by HCMV UL97, a homologue of UL13. These results indicate that the posttranslational modification of EF-1delta is conserved herpesvirus function and the UL13 homologues may be responsible for the universal modification of the translation factor.
Kawaguchi
Y
Y
Department of Tumor Virology, Division of Virology and Immunology, Medical Research Institute, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Matsumura
T
T
Roizman
B
B
Hirai
K
K
D018138:Alphaherpesvirinae / Q000502:physiology*; D000818:Animals; D018141:Betaherpesvirinae / Q000502:physiology*; D002415:Cats; D002417:Cattle; D002460:Cell Line; D002522:Chlorocebus aethiops; D018142:Gammaherpesvirinae / Q000502:physiology*; D006736:Horses; D006801:Humans; D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors / Q000378:metabolism*; D010766:Phosphorylation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D014709:Vero Cells
D020648:Peptide Elongation Factor 1; D010445:Peptide Elongation Factors; D011494:Protein Kinases; D017853:Phosphotransferases (Alcohol Group Acceptor); C082380:UL13 protein, Simplexvirus; C075365:ganciclovir kinase