title: Reduced glucose availability attenuates circadian responses to light in mice.
abstract: To test whether circadian responses to light are modulated by decreased glucose availability, we analyzed photic phase resetting of the circadian rhythm of locomotor activity in mice exposed to four metabolic challenges: 1) blockade of glucose utilization induced by 2-deoxy-D-glucose (2-DG), 2) fasting (food was removed for 30 h), 3) insulin administration, and 4) insulin treatment after fasting. In mice housed in constant darkness, light pulses applied during early subjective night induced phase delays of the rhythm of locomotor activity, whereas light pulses applied during late subjective night caused phase advances. There was an overall reduction of light-induced phase shifts, with a more pronounced effect for delays, in mice pretreated with 500 mg/kg ip 2-DG compared with mice injected with saline. Administration of glucose with 2-DG prevented the reduction of light-induced phase delays. Furthermore, phase delays were reduced in fed mice pretreated with 5 IU/kg sc insulin and in fasted mice injected with saline or insulin compared with control fed mice. These results show that circadian responses to light are reduced when brain glucose availability is decreased, suggesting a metabolic modulation of light-induced phase shifts.
authors: Center for Circadian Biology and Medicine, Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA.e-challet@nwu.edu
authors: E
authors: E
authors: Challet
authors: S
authors: S
authors: Losee-Olson
authors: F W
authors: FW
authors: Turek
mesh_terms: D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001786:Blood Glucose / Q000032:analysis; D001921:Brain / Q000378:metabolism*; D002940:Circadian Rhythm / Q000528:radiation effects*; D003847:Deoxyglucose / Q000494:pharmacology; D005215:Fasting; D005947:Glucose / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D008027:Light; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology / Q000528:radiation effects
chemical_list: D000963:Antimetabolites; D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D003847:Deoxyglucose; D005947:Glucose