The Sox-13 gene: structure, promoter characterization, and chromosomal localization.
We have recently identified the HMG box transcription factor Sox-13 and described its expression during murine embryogenesis. Here we describe the structure of the murine Sox-13 gene. This gene spans approximately 12 kb and consists of 13 exons. The HMG domain is encoded by exons XI and XII, separated by an intron that is conserved among Sox-5, Sox-13, and Sox-17. A single major transcription initiation site was identified. Deletion analysis of the 3-kb promoter region revealed a 400-bp fragment driving transcription of a luciferase reporter in a Sox-13-expressing cell line. To determine the chromosomal localization of the human gene, a human SOX13 cDNA was isolated with 75% homology to the mouse Sox-13. FISH analysis mapped the human SOX13 gene to chromosome 1 band q32.
Roose
J
J
Department of Immunology, University Hospital Utrecht, Utrecht, 3508 GA, The Netherlands. J.Roose@lab.azu.nl
Korver
W
W
de Boer
R
R
Kuipers
J
J
Hurenkamp
J
J
Clevers
H
H
D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D002874:Chromosome Mapping; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D004247:DNA / Q000737:chemistry / Q000235:genetics; D005091:Exons; D005786:Gene Expression Regulation; D006609:High Mobility Group Proteins / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007438:Introns; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012313:RNA / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D055755:SOXD Transcription Factors; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
D001324:Autoantigens; D006609:High Mobility Group Proteins; D011993:Recombinant Fusion Proteins; C491720:SOX13 protein, human; D055755:SOXD Transcription Factors; C110455:Sox13 protein, mouse; D012313:RNA; D004247:DNA; D008156:Luciferases