Down-regulation of nucleophosmin/B23 mRNA delays the entry of cells into mitosis.
In investigating the regulation of nucleophosmin/B23 mRNA expression at the entry of mitosis, the results of Northern gel blot analysis showed that the nucleophosmin/B23 mRNA levels significantly increased in prometaphase (nocodazole-arrested) or metaphase (colchicine-arrested) cells collected by mitotic shake-off. A higher level of nucleophosmin/B23 mRNA was detected in all the collected mitotic cells arrested by treatment with nocodazole for 10-18h as compared to that in G2 cells. An attempt was then made to determine whether the regulation of nucleophosmin/B23 mRNA plays a role in the control of entry into mitosis. Down-regulation of nucleophosmin/B23 mRNA by transfection of its antisense construct resulted in the delay of cells entering mitosis. The demonstration of the characteristic changes in the mRNA level of nucleophosmin/B23 during the entry of cells into mitosis implicates the importance of nucleophosmin/B23 in the control of the mitotic fate of nucleoli and cell growth.
Jiang
P S
PS
Cancer Biochemistry Laboratory, Department of Pharmacology, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-San, Tao-Yuan 333, Taiwan, Republic of China.
Yung
B Y
BY
D016375:Antisense Elements (Genetics) / Q000235:genetics; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D015536:Down-Regulation; D006367:HeLa Cells; D006801:Humans; D007399:Interphase / Q000187:drug effects; D008677:Metaphase / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D008940:Mitotic Index / Q000187:drug effects; D015739:Nocodazole / Q000494:pharmacology; D009687:Nuclear Proteins / Q000235:genetics*; D000090243:Nucleophosmin; D012333:RNA, Messenger / Q000378:metabolism; D013936:Thymidine / Q000494:pharmacology; D013997:Time Factors; D014162:Transfection
D016375:Antisense Elements (Genetics); C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D000090243:Nucleophosmin; D015739:Nocodazole; D003078:Colchicine; D013936:Thymidine