Oncogene activation in myeloid leukemias by Graffi murine leukemia virus proviral integration.
The Graffi murine leukemia virus (MuLV) is a nondefective retrovirus that induces granulocytic leukemia in BALB/c and NFS mice. To identify genes involved in Graffi MuLV-induced granulocytic leukemia, tumor cell DNAs were examined for genetic alterations at loci described as common proviral integration sites in MuLV-induced myeloid, lymphoid, and erythroid leukemias. Southern blot analysis revealed rearrangements in c-myc, Fli-1, Pim-1, and Spi-1/PU.1 genes in 20, 10, 3.3, and 3.3% of the tumors tested, respectively. These results demonstrate for the first time the involvement of those genes in granulocytic leukemia.
Denicourt
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Laboratoire de Biologie Moléculaire, Département des Sciences Biologiques, Université du Québec à Montréal, Montréal, Québec H3C 3P8, Canada.
Edouard
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Rassart
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D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics*; D015321:Gene Rearrangement; D009052:Leukemia Virus, Murine / Q000235:genetics*; D007951:Leukemia, Myeloid / Q000235:genetics* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009857:Oncogenes; D017346:Protein Serine-Threonine Kinases; D051789:Proto-Oncogene Protein c-fli-1; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics*; D051573:Proto-Oncogene Proteins c-pim-1; D011533:Proviruses / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics*; D016662:Virus Integration / Q000235:genetics*; D000099232:Proto-Oncogene Protein Spi-1
D004268:DNA-Binding Proteins; D017346:Protein Serine-Threonine Kinases; D051789:Proto-Oncogene Protein c-fli-1; D011518:Proto-Oncogene Proteins; D016271:Proto-Oncogene Proteins c-myc; D051573:Proto-Oncogene Proteins c-pim-1; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1; C497426:Fli1 protein, mouse; C496636:Pim1 protein, mouse