Genotoxic evaluation of the antimicrobial drug, trimethoprim, in cultured human lymphocytes.
The antimicrobial drug, trimethoprim, was evaluated for genotoxicity in human peripheral blood lymphocyte cultures set-up from two healthy donors. Sister-chromatid exchanges (SCE) and micronuclei (MN) were scored as genetic endpoints. The treatment was done using different trimethoprim concentrations ranging from 1 to 100 microg/ml. From our results, we can conclude that this drug is able to induce both cytotoxic and moderate genotoxic effects, as revealed by the increases seen in SCE and MN frequencies in cultures from the two donors and, at least, at one of the concentrations tested.
Abou-Eisha
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Grup de Mutagènesi, Unitat de Genètica, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Edifici Cn, 08193 Bellaterra (Cerdanyola del Vallés), Spain.
Creus
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Marcos
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R
D000892:Anti-Infective Agents, Urinary / Q000633:toxicity; D002478:Cells, Cultured; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D048629:Micronuclei, Chromosome-Defective / Q000187:drug effects* / Q000235:genetics; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012854:Sister Chromatid Exchange / Q000187:drug effects* / Q000235:genetics; D014295:Trimethoprim / Q000633:toxicity*
D000892:Anti-Infective Agents, Urinary; D009153:Mutagens; D014295:Trimethoprim