Gene targeting and somatic cell genetics--a rebirth or a coming of age?
A number of recent advances have significantly facilitated gene targeting in somatic cells. Gene targeting can now be performed with the same ease and efficiency in somatic cells as in murine embryonic stem cells. Rigorous genetic analyses can therefore be applied for the first time to the large number of excellent human cell culture systems. These tools will be important in areas where rodent models do not adequately represent human biology.
Sedivy
J M
JM
Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI 02912, USA. john_sedivy@brown.edu
Dutriaux
A
A
D000818:Animals; D002460:Cell Line; D004247:DNA; D018390:Gene Targeting; D005822:Genetic Vectors; D006801:Humans; D006822:Hybrid Cells; D051379:Mice
D004247:DNA