title: Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.
abstract: Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily unrelated and together represent almost all of the alphaHA deh genes described to date. We report the design and evaluation of degenerate PCR primer pairs for the separate amplification and isolation of group I and II deh genes. Amino acid sequences derived from 10 of 11 group I deh partial gene products of new and previously reported bacterial isolates showed conservation of five residues previously identified as essential for activity. The exception, DehD from a Rhizobium sp., had only two of these five residues. Group II deh gene sequences were amplified from 54 newly isolated strains, and seven of these sequences were cloned and fully characterized. Group II dehalogenases were stereoselective, dechlorinating L- but not D-2-chloropropionic acid, and derived amino acid sequences for all of the genes except dehII degrees P11 showed conservation of previously identified essential residues. Molecular analysis of the two deh families highlighted four subdivisions in each, which were supported by high bootstrap values in phylogenetic trees and by enzyme structure-function considerations. Group I deh genes included two putative cryptic or silent genes, dehI degrees PP3 and dehI degrees 17a, produced by different organisms. Group II deh genes included two cryptic genes and an active gene, dehIIPP3, that can be switched off and on. All alphaHA-degrading bacteria so far described were Proteobacteria, a result that may be explained by limitations either in the host range for deh genes or in isolation methods.
authors: Cardiff School of Biosciences, Cardiff University, Cardiff, CF1 3TL, Wales, United Kingdom.
authors: K E
authors: KE
authors: Hill
authors: J R
authors: JR
authors: Marchesi
authors: A J
authors: AJ
authors: Weightman
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000201:enzymology*; D002713:Chlorine / Q000378:metabolism; D017124:Conserved Sequence; D017931:DNA Primers; D004275:DNA, Ribosomal / Q000235:genetics; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D006867:Hydrolases / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011422:Propionates / Q000378:metabolism; D011487:Protein Conformation; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D004275:DNA, Ribosomal; D011422:Propionates; D012336:RNA, Ribosomal, 16S; D002713:Chlorine; D006867:Hydrolases; C034507:2-haloacid dehalogenase