[Interstrain differences in enzymatic activity of CYP2B1 and expression of its gene in the rat liver during induction with phenobarbital and triphenyldioxane].
The interstrain differences in hepatic 7-pentoxyresorufin-O-dealkylase and 16 beta-androstendione hydroxylase activities specific for cytochrome P450 2B1 have been found in Sprague-Dawley, Brattleboro and Wistar rats treated with with phenobarbital, triphenyldioxane. Similar differences were found in the accumulation of corresponding mRNAs suggesting differences in expression of cytochrome P450 gene. Analysis of the enzymatic activity and mRNA level revealed positive correlation between these parameters. Thus, differences in transcriptional activity of CYP2B1 gene during induction by phenobarbital and triphenyldioxane may be one of reasons for interstrain differences between enzymatic activities of rat liver cytochrome P4502B1.
Shvarts
E I
EI
Institute of Molecular Pathology and Ecological Biochemistry, Siberian Branch RAMS, Novosibirsk. IMPEB@CYBER.MA.NSC.RU
Guliaeva
L F
LF
Liakhovich
V V
VV
D000818:Animals; D019362:Cytochrome P-450 CYP2B1 / Q000096:biosynthesis* / Q000235:genetics; D004146:Dioxanes / Q000494:pharmacology*; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats; D011910:Rats, Brattleboro; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D013045:Species Specificity
D004146:Dioxanes; C054589:2,4,6-triphenyl-1,3-dioxane; D019362:Cytochrome P-450 CYP2B1; D010634:Phenobarbital