Synthesis of polystyrene nanospheres having lactose-conjugated hydrophilic polymers on their surfaces and carbohydrate recognition by proteins.
We prepared a poly(vinylamine)-grafted polystyrene nanosphere by the free radical polymerization of poly(N-vinylacetamide) macromonomer and styrene with AIBN in ethanol and subsequent acid hydrolysis. We conjugated lactose on the nanosphere by an amide linkage and found that the lactose was recognized by a galactose-specific lectin RCA120, which was analyzed by enzyme-linked lectin assay (ELLA). The binding ability of the lectin to lactose on the nanosphere was at least 10(2) times larger than that to a monomeric lactose. Binding ability was clearly affected by the lactose density on the nanosphere and showed a maximum value at the suitable surface density of lactose. We found that the carbohydrate-conjugated nanosphere would be a useful biomaterial for a site specific drug carrier.
Serizawa
T
T
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Japan.
Uchida
T
T
Akashi
M
M
D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D006868:Hydrolysis; D007783:Lactones / Q000737:chemistry; D007785:Lactose / Q000737:chemistry*; D037102:Lectins / Q000378:metabolism; D008863:Microspheres; D037121:Plant Lectins; D011137:Polystyrenes / Q000138:chemical synthesis*; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
D002241:Carbohydrates; D007783:Lactones; D037102:Lectins; D037121:Plant Lectins; D011137:Polystyrenes; D011506:Proteins; C017707:Ricinus communis agglutinin-1; D007785:Lactose