Effect of electroacupuncture on the activities of tyrosine protein kinase in subcellular fractions of activated T lymphocytes from the traumatized rats.
The present study was to observe the dynamic changes of tyrosine protein kinase (TPK) activity in subcellular fractions in the early stage of activation of T lymphocytes from normal and traumatized rats, and the regulatory effect of electroacupuncture (EA) stimulation on it. The results showed that the activities of TPK in membranous and cytosolic fractions of activated T lymphocytes were increased on second 5, and the peak was on scond 45 after ConA stimulation. Then it was decreased gradually. Comparing with the control group, the activities of TPK in membranous and cytosolic fractions of activated T lymphocytes from the traumatized rats were inhibited in various degrees especially in membrane. EA of "Zusanli"(ST-36) and "Lanwei"(Extra 33) points could enhance the activity of TPK in subcellular fractions of activated T lymphocytes from the traumatized rats. The results indicated that EA stimulation could prevent the inhibition of activation of TPK induced by trauma stress, and contribute to transmembrane signal transduction of T lymphocytes.
Cheng
X D
XD
State Key Laboratory of Medical Neurobiology, Shanghai Medical University, PR China.
Wu
G C
GC
He
Q Z
QZ
Cao
X D
XD
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D004195:Disease Models, Animal; D015671:Electroacupuncture; D008213:Lymphocyte Activation; D008297:Male; D011505:Protein-Tyrosine Kinases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction; D013312:Stress, Physiological / Q000209:etiology / Q000276:immunology* / Q000628:therapy*; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology / Q000648:ultrastructure; D014947:Wounds and Injuries / Q000150:complications*
D011505:Protein-Tyrosine Kinases