title: Cyclic AMP induces functional presynaptic boutons in hippocampal CA3-CA1 neuronal cultures.
abstract: Long-term forms of synaptic plasticity that may underlie learning and memory have been suggested to depend on changes in the number of synapses between presynaptic and postsynaptic neurons. Here we have investigated a form of synaptic plasticity in cultures of hippocampal CA3 and CA1 neurons related to the late phase of long-term potentiation, which depends on cAMP and protein synthesis. Using the fluorescent dye FM 1-43 to label active presynaptic terminals, we find that a membrane permeable analog of cAMP enhances the number of active presynaptic terminals and that this effect requires protein synthesis.
authors: Department of Pharmacology, Columbia University, New York, New York 10032, USA.
authors: L
authors: L
authors: Ma
authors: L
authors: L
authors: Zablow
authors: E R
authors: ER
authors: Kandel
authors: S A
authors: SA
authors: Siegelbaum
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000494:pharmacology / Q000502:physiology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005456:Fluorescent Dyes; D006624:Hippocampus / Q000166:cytology / Q000502:physiology*; D017774:Long-Term Potentiation / Q000502:physiology; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology*; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000502:physiology; D013873:Thionucleotides / Q000494:pharmacology
chemical_list: D018691:Excitatory Amino Acid Antagonists; C073804:FM1 43; D005456:Fluorescent Dyes; D011500:Protein Synthesis Inhibitors; D011726:Pyridinium Compounds; D000644:Quaternary Ammonium Compounds; D017470:Receptors, Glutamate; D013873:Thionucleotides; C016957:adenosine-3',5'-cyclic phosphorothioate; D000841:Anisomycin; D000242:Cyclic AMP