Long-term effects of repetitive exposure to a static magnetic field (1.5 T) on proliferation of human fetal lung fibroblasts.
The aim of the study was to assess the effects of repetitive exposures to a static magnetic field (1.5 T) on human fetal lung fibroblast (HFL) proliferation. HFL were exposed three times a week for 1 hr to a static magnetic field for 3 weeks. Cells were subcultured every week. Population doublings (PD) and cumulative population doublings (CPD) were calculated weekly. Colony formation assays, bromodeoxyuridine enzyme-linked immunosorbent assay, and cell cycle analysis were performed weekly. After the third week, proliferation kinetics were assessed. Over a period of 3 weeks no statistically significant differences between the PD and CPD of exposed and control cells could be detected. Clonogenic activity, DNA synthesis, cell cycle, and proliferation kinetics were not altered by magnetic field exposure. The data do not provide evidence that repetitive exposures to a static magnetic field (1.5 T) exert effects on HFL proliferation.
Wiskirchen
J
J
Department of Radiology, Eberhard-Karls-Universität Tübingen, Germany. jakub.wiskirchen@uni-tuebingen.de
Groenewaeller
E F
EF
Kehlbach
R
R
Heinzelmann
F
F
Wittau
M
M
Rodemann
H P
HP
Claussen
C D
CD
Duda
S H
SH
D002455:Cell Division / Q000528:radiation effects; D002478:Cells, Cultured / Q000528:radiation effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005333:Fetus / Q000528:radiation effects; D005347:Fibroblasts / Q000166:cytology / Q000528:radiation effects*; D006801:Humans; D008168:Lung / Q000033:anatomy & histology / Q000166:cytology / Q000196:embryology*; D008279:Magnetic Resonance Imaging / Q000009:adverse effects* / Q000379:methods; D012016:Reference Values; D018709:Statistics, Nonparametric; D013997:Time Factors