title: Neoperitoneal formation after implantation of various biomaterials for the repair of abdominal wall defects in rabbits.
abstract: OBJECTIVE
  To study the interfaces between the visceral peritoneum and some of the biomaterials used to repair defects in the abdominal wall.
  
  
  METHODS
  Animal study.
  
  
  METHODS
  School of medicine, Spain.
  
  
  METHODS
  48 New Zealand white rabbits divided into 4 groups of 12 each.
  
  
  METHODS
  Full thickness defects 50 x 70 mm were created in the abdominal wall and repaired with polytetrafluoroethylene (PTFE, Soft Tissue Patch), one of two polypropylene patches (Marlex and Prolene), or lyophylised dura mater (Lyo-Dura). 3 animals from each group were killed at 14, 30, 60 and 90 days and specimens examined by light microscopy, scanning electron microscopy, and immuno histochemistry by labelling of macrophages with RAM-11, a specific monoclonal antibody (MoAb).
  
  
  METHODS
  Infection, healing, development of adhesions, and histological appearance of the interface.
  
  
  RESULTS
  Tissues responded similarly to materials of similar structures. Layered prostheses (PTFE and Lyo-Dura) caused formation of a well organised neoperitoneum with few adhesions to the abdominal viscera (loose adhesions in 2 animals in each group), whereas the mesh prostheses generated a disorganised neoperitoneum with many adhesions (Marlex loose adhesions 3, firm 8, and integrated 1; Prolene loose adhesions 2, firm 8, and integrated 2). Lyo-Dura was associated with the formation of areas of calcification. Labelling of macrophages with the MoAb showed that they were in direct contact with all materials studied.
  
  
  CONCLUSIONS
  Layered biomaterials with little or no porosity (PTFE and Lyo-Dura) are the most suitable of the four for implantation in sites where the prosthesis is in contact with the visceral peritoneum, because they induce minimal adhesions.
authors: Department of Morphological Sciences and Surgery, Faculty of Medicine, University of Alcala de Henares, Madrid, Spain.
authors: J M
authors: JM
authors: Bellón
authors: L A
authors: LA
authors: Contreras
authors: G
authors: G
authors: Pascual
authors: J
authors: J
authors: Bujan
mesh_terms: D000009:Abdominal Muscles / Q000002:abnormalities* / Q000601:surgery*; D000818:Animals; D001672:Biocompatible Materials; D008297:Male; D010537:Peritoneum / Q000648:ultrastructure; D011095:Polyethylenes; D011126:Polypropylenes; D011138:Polytetrafluoroethylene; D019736:Prostheses and Implants; D011817:Rabbits; D013526:Surgical Mesh; D000267:Tissue Adhesions
chemical_list: D001672:Biocompatible Materials; C016674:Plastipore; D011095:Polyethylenes; D011126:Polypropylenes; D011138:Polytetrafluoroethylene