The Drosophila melanogaster 60A chromosomal division is extremely dense with functional genes: their sequences, genomic organization, and expression.
We cloned and sequenced genomic DNA contigs spanning over 45 kb, surrounding the insertion site of the P-element that is responsible for the developmental defects in the ken and barbie (ken) mutant of Drosophila. This region harbors 10 functional transcription units, in addition to the already well-characterized TGFbeta-60A gene. These include the genes, undefined 1 (UD1), UD2, and UD3, each coding for proteins of unknown function, the ken gene encoding a new Krüppel-like putative transcription factor, the fly homologues of the mammalian mitochondrial trifunctional enzyme (thiolase), and the TAR DNA-binding protein-43 (TBPH), the first nonvertebrate member of the transmembrane 4 superfamily (TM4SF) gene, a new homeodomain gene, and a gene coding for a putative nuclear binding protein (PNBP) that is homologous to maleless, and a Copia-like element. UD3 exists in an intron of the maleless homologue, yet is expressed independent of it. The UD1 and TM4SF genes orient in a tail-to-tail manner with their 3' untranslated region sequences overlapping over 44 nucleotides. Thus the partial overlap and intraintronic organization permitted dense packing of the functional genes within a short segment of the genome.
Lukacsovich
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ERATO Yamamoto Behavior Genes Project, Mitsubishi Kasei Institute of Life Sciences, Machida, 194-8511, Japan.
Asztalos
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Juni
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Awano
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Yamamoto
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D000101:Acetyl-CoA C-Acetyltransferase / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D004268:DNA-Binding Proteins / Q000235:genetics; D016208:Databases, Factual; D029721:Drosophila Proteins / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D020224:Expressed Sequence Tags; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics; D017403:In Situ Hybridization; D008957:Models, Genetic; D008969:Molecular Sequence Data; D005810:Multigene Family; D015183:Restriction Mapping; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D016212:Transforming Growth Factor beta / Q000235:genetics
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; C000589036:TBPH protein, Drosophila; D016212:Transforming Growth Factor beta; D000101:Acetyl-CoA C-Acetyltransferase