Effect of dentin bonding agents on the secretion of inflammatory mediators from macrophages.
Dentin bonding agents (DBAs) have been proposed as substitutes for amalgam as root-end filling materials. The current study tested the hypothesis that certain components of DBAs could alter the secretion of cytokines from macrophages. Such alteration would likely be undesirable for healing of the periapical tissues. Human THP-1 macrophages were exposed to 2-hydroxyethyl methacrylate, 4-methacryloxyethyl trimelliate anhydride, bisphenol-gycidylmethacrylate, and urethane dimethacrylate. The secretion of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) were measured with or without challenge by lipopolysaccharide (LPS). Results showed that all DBA components completely suppressed LPS-induced IL-1 beta and TNF-alpha secretion at concentrations that suppressed mitochondrial activity by 50%. In addition, 4-methacryloxyethyl trimelliate anhydride induced secretion of IL-1 beta, but not TNF-alpha, without the LPS challenge. These results indicate that DBA components may alter normal macrophage-directed inflammatory responses if the macrophages are exposed to sufficiently high concentrations of these components.
Rakich
D R
DR
Department of Endodontics, School of Dentistry, Medical College of Georgia, Augusta 30912-1260, USA.
Wataha
J C
JC
Lefebvre
C A
CA
Weller
R N
RN
D000180:Acrylic Resins / Q000009:adverse effects; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate / Q000009:adverse effects; D002460:Cell Line; D017220:Dentin-Bonding Agents / Q000009:adverse effects*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects; D018836:Inflammation Mediators / Q000378:metabolism*; D007375:Interleukin-1 / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D008689:Methacrylates / Q000009:adverse effects; D011140:Polyurethanes / Q000009:adverse effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
D000180:Acrylic Resins; D017220:Dentin-Bonding Agents; D018836:Inflammation Mediators; D007375:Interleukin-1; D008070:Lipopolysaccharides; D008689:Methacrylates; D011140:Polyurethanes; D014409:Tumor Necrosis Factor-alpha; C029824:urethane dimethacrylate luting resin; D017438:Bisphenol A-Glycidyl Methacrylate; C005044:hydroxyethyl methacrylate; C031172:4-META resin