[Detection of ABO genotypes by simultaneous PCR-RFLP method].
OBJECTIVE
To study human ABO genotyping by means of multiplex PCR and restriction fragment length polymorphism.


METHODS
Two specific fragments of ABO gene were simultaneously amplified in a single tube, and then the double restriction digestion with RE Kpn I and Alu I was performed in the same tube. The amplified products were analyzed by PAGE and silver staining. A total of 125 Han unrelated individuals living in Wuhan were genotyped.


RESULTS
Six genotypes of ABO were detected and the distribution was in good agreement with Hardy-Weinberg equilibrium.


CONCLUSIONS
The results demonstrate that PCR-RFLP based approach is convenient, reliable and should be of value in forensic application.
Yang
Q
Q
Department of Forensic Medicine, Tongji Medical University, Wuhan, 430030, P. R. China. fywz@tjmu.edu.cn
Zhu
C
C
D000017:ABO Blood-Group System / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
D000017:ABO Blood-Group System