[Perinatal ischemic brain damages and apoptosis].
Perinatal brain damages are caused mainly by circulation insufficiency due to intrauterine or birth asphyxia. Hypoxic-ischemic encephalopathy (HIE) and pontosubicular neuronal necrosis (PSN) are typical perinatal ischemic diseases. We investigated the expression of apoptosis in these diseases, using TdT-mediated dUTP nick end labeling (TUNEL) method and immunohistochemistry with Bcl-2, Bcl-x, Bak and caspase 3 (CPP 32). In HIE, Purkinje cells showed overexpression of Bcl-2 and CPP 32, and some karyorrhectic cells were positive for TUNEL. In PSN, neurons of the pontine nuclei showed overexpression of Bcl-x and CPP 32, and most karyorrhectic neurons were positive for TUNEL. Immature neurons were susceptible to these changes. Apoptosis may play an important role in the pathomechanism of perinatal ischemic brain damages.
Itoh
M
M
Department of Mental Retardation and Birth Defect Research, National Center of Neurology and Psychiatry, Tokyo.
D017209:Apoptosis; D001238:Asphyxia Neonatorum / Q000473:pathology; D002545:Brain Ischemia / Q000473:pathology*; D002531:Cerebellum / Q000196:embryology; D002543:Cerebral Hemorrhage / Q000473:pathology; D005311:Fetal Hypoxia / Q000473:pathology; D006801:Humans; D002534:Hypoxia, Brain / Q000473:pathology; D007231:Infant, Newborn; D009336:Necrosis; D009410:Nerve Degeneration