Distinct structures and functions of related pre- and postsynaptic carbohydrates at the mammalian neuromuscular junction.
Carbohydrates that terminate in beta-linked N-acetylgalactosamine (betaGalNAc) residues are concentrated in the postsynaptic apparatus of the skeletal neuromuscular junction and have been implicated in the differentiation of the postsynaptic membrane. We now report that distinct synapse-specific betaGalNAc-containing carbohydrates are associated with motor nerve terminals. Two monoclonal antibodies that recognize distinct betaGalNAc-containing epitopes, CT1 and CT2, both stain synaptic sites on skeletal muscle fibers. However, CT1 selectively stains nerve terminal, whereas CT2 selectively stains the postsynaptic apparatus. Likewise, CT1 and CT2 selectively stain motoneuron-like and muscle cell lines, respectively. Using the cell lines, we identify distinct CT1- and CT2-reactive glycolipids and glycoproteins. Finally, we show that GalNAc modulates the adhesion of motoneuron-like cells to recombinant fragments of a synaptic cleft component, laminin beta2. Together, these results show that pre- as well as postsynaptic membranes bear and are affected by distinct but related synapse-specific carbohydrates.
Martin
P T
PT
Department of Neurosciences, University of California at San Diego, School of Medicine, 9500 Gilman Drive, La Jolla, California 92093-0691, USA.pmartin@ucsd.edu
Scott
L J
LJ
Porter
B E
BE
Sanes
J R
JR
D000116:Acetylgalactosamine / Q000032:analysis*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002448:Cell Adhesion; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D007797:Laminin / Q000032:analysis; D037102:Lectins; D009045:Motor Endplate / Q000737:chemistry*; D009046:Motor Neurons / Q000737:chemistry / Q000166:cytology; D024510:Muscle Development; D009124:Muscle Proteins / Q000737:chemistry*; D018482:Muscle, Skeletal / Q000196:embryology / Q000254:growth & development / Q000294:innervation; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry*; D051381:Rats; D017661:Receptors, Presynaptic / Q000737:chemistry*; D013329:Structure-Activity Relationship
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006017:Glycolipids; D006023:Glycoproteins; D007797:Laminin; D037102:Lectins; D009124:Muscle Proteins; D009419:Nerve Tissue Proteins; D009841:Oligonucleotides; D017661:Receptors, Presynaptic; C058783:laminin beta2; D000116:Acetylgalactosamine