Prohibitin and RACK homologues are up-regulated in trypanosomes induced to undergo apoptosis and in naturally occurring terminally differentiated forms.
Two genes have been identified as up-regulated late during ConA-induced apoptosis in procyclic form Trypanosoma brucei rhodesiense. The first represents a homologue of prohibitin, a proto-oncogene originally described in mammals and subsequently in yeast, which is involved in cell-cycle control and senescence. The Trypanosoma prohibitin homologue appears to contain within it a putative death domain. The second gene, homologous to a family of regulatory proteins which are receptors for activated protein kinase C (RACKs), is also shown to be up-regulated in terminally differentiated bloodstream form trypanosomes. These are the first endogenous genes to be identified as up-regulated in programmed cell death (PCD) in unicellular organisms.
Welburn
S C
SC
Tsetse Research Group, Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, UK. sue.welburn@tsetse.demon.co.uk
Murphy
N B
NB
D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D001665:Binding Sites; D015152:Blotting, Northern; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D000091402:Prohibitins; D011467:Prostate; D011506:Proteins / Q000235:genetics*; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface / Q000235:genetics*; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D013997:Time Factors; D014346:Trypanosoma brucei brucei / Q000187:drug effects / Q000235:genetics*; D015854:Up-Regulation
C000715470:MAS1 protein, human; D000091402:Prohibitins; D011506:Proteins; D000090063:Proto-Oncogene Mas; D012333:RNA, Messenger; D000075370:Receptors for Activated C Kinase; D011956:Receptors, Cell Surface; D012097:Repressor Proteins; D003208:Concanavalin A