title: Timing of cyclin E gene expression depends on the regulated association of a bipartite repressor element with a novel E2F complex.
abstract: Transient induction of the cyclin E gene in late G1 gates progression into S. We show that this event is controlled via a cyclin E repressor module (CERM), a novel bipartite repressor element located near the cyclin E transcription start site. CERM consists of a variant E2F-binding site and a contiguous upstream AT-rich sequence which cooperate during G0/G1 to delay cyclin E expression until late G1. CERM binds the protein complex CERC, which disappears upon progression through G0-G1 and reappears upon entry into the following G1. CERC disappearance correlates kinetically with the liberation of the CERM module in vivo and cyclin E transcriptional induction. CERC contains E2F4/DP1 and a pocket protein, and sediments faster than classical E2F complexes in a glycerol gradient, suggesting the presence of additional components in a novel high molecular weight complex. Affinity purified CERC binds to CERM but not to canonical E2F sites, thus displaying behavior different from known E2F complexes. In cells nullizygous for members of the Rb family, CERC is still detectable and CERM-dependent repression is functional. Thus p130, p107 and pRb function interchangeably in CERC. Notably, the CERC-CERM complex dissociates prematurely in pRb-/- cells in correspondence with the premature expression of cyclin E. Thus, we identify a new regulatory module that controls repression of G1-specific genes in G0/G1.
authors: Institut de Génétique Moléculaire, UMR 5535, CNRS, 1919 route de Mende, 34293, Montpellier cedex 5, France.
authors: L
authors: L
authors: Le Cam
authors: J
authors: J
authors: Polanowska
authors: E
authors: E
authors: Fabbrizio
authors: M
authors: M
authors: Olivier
authors: A
authors: A
authors: Philips
authors: E
authors: E
authors: Ng Eaton
authors: M
authors: M
authors: Classon
authors: Y
authors: Y
authors: Geng
authors: C
authors: C
authors: Sardet
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D019927:Cyclin E / Q000235:genetics*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050704:E2F4 Transcription Factor; D016193:G1 Phase / Q000235:genetics* / Q000502:physiology; D015870:Gene Expression; D005809:Genes, Regulator; D046911:Macromolecular Substances; D051379:Mice; D008969:Molecular Sequence Data; D016160:Retinoblastoma Protein / Q000235:genetics / Q000378:metabolism; D056465:Retinoblastoma-Binding Protein 1; D050680:Transcription Factor DP1; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: C533431:Arid4a protein, mouse; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D019927:Cyclin E; D017931:DNA Primers; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D050704:E2F4 Transcription Factor; C493763:E2f4 protein, mouse; D046911:Macromolecular Substances; D016160:Retinoblastoma Protein; D056465:Retinoblastoma-Binding Protein 1; C493736:Tfdp1 protein, mouse; D050680:Transcription Factor DP1; D014157:Transcription Factors; D004247:DNA