title: [HLA-DQA1 genes involved in the genetic susceptibility to duodenal ulcer in Wuhan Hans].
abstract: OBJECTIVE
  To study the genetic susceptibility of HLA-DQA1 alleles to duodenal ulcer in Chinese Hans from Wuhan and its nearby regions.
  
  
  METHODS
  Seventy patients with duodenal ulcer and fifty healthy controls were examined for HLA-DQA1 genotypes. HLA-DQA1 typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific restriction enzymes (PCR-RFLP), Apal I, Basj I, Hph I, Fok I, Mbo II and Mnl I.
  
  
  RESULTS
  The allele frequency of DQA1 0301 in patients with duodenal ulcer (64.3%) was significantly higher than that in healthy controls (36%). In contrast, the allele frequency of DQA1 0102 in patients with duodenal ulcer (8.6%) was significantly lower than that in healthy controls (26%).
  
  
  CONCLUSIONS
  These findings suggest that DQA1 0301 is a susceptible gene for duodenal ulcer in Wuhan Hans while DQA1 0102 is its resistant gene, and there are immunogenetic differences in HLA-DQA1 locus between duodenal ulcer patients and healthy controls.
authors: Digestive Department of the Second Hospital of Hubei Medical University, Wuhan, 430071, P. R. China.
authors: Y
authors: Y
authors: Du
authors: C
authors: C
authors: Deng
authors: D
authors: D
authors: Lu
authors: M
authors: M
authors: Huang
authors: S
authors: S
authors: Guo
authors: W
authors: W
authors: Hou
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D004381:Duodenal Ulcer / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059848:HLA-DQ alpha-Chains; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D006683:HLA-DQ Antigens; D059848:HLA-DQ alpha-Chains; C069050:HLA-DQA1 antigen