The upregulation of TNF alpha production is not a generalised phenomenon in the elderly between their sixth and seventh decades of life.
The aim of this paper was to analyse the link between the intensity of tumor necrosis factor alpha (TNF alpha) production and the health status of the elderly and to find out whether the age between sixty and seventy is a 'turning point' for the changes in the production of this cytokine. Fifty elderly volunteers (age range: 60-70), twenty-five middle-aged (age range: 36-59) and fifty young (age range: 20-35) were enrolled into the study. Their health status was graded as 'healthy' and 'almost-healthy'. The level of TNF alpha was determined by bioassay, the activity of the TNF alpha gene was analysed in non-stimulated PBMC by the RT-PCR method. The results showed that the production of TNF alpha in the 'healthy' elderly people is not upregulated until the age of sixty-seventy. The 'almost-healthy' elderly are characterised by a higher release of TNF alpha from the cultures of non- and stimulated PBMC and an activation of the TNF alpha gene in the non-stimulated PBMC. Summarising, our results indicate that the deterioration of health status is a prerequisite for an exaggerated TNF alpha production by peripheral blood mononuclear cells of people between the sixth and seventh decades of life.
Myśliwska
J
J
Department of Histology and Immunology, Medical University of Gdańsk, Poland. jolmys@amedec.amg.gda.pl
Bryl
E
E
Foerster
J
J
Myśliwski
A
A
D000328:Adult; D000368:Aged; D000375:Aging / Q000097:blood*; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D006304:Health Status; D006801:Humans; D020014:K562 Cells / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D012313:RNA / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation / Q000502:physiology*
D003470:Culture Media; D017931:DNA Primers; D008934:Mitogens; D014409:Tumor Necrosis Factor-alpha; D012313:RNA