Cutting edge: phorbol ester induction of IFN-gamma receptors leads to enhanced DR alpha gene expression.
We observed that IFN-gamma-inducible expression of the DR alpha gene was enhanced when THP-1 cells are differentiated into macrophage-like cells by phorbol ester treatment. Here, we observed that class II MHC trans-activator and STAT1 alpha mRNA, mediators of the signaling cascade from the IFN-gamma receptor to the DR alpha induction, were markedly increased by IFN-gamma stimulation in phorbol ester-activated THP-1 cells; however, both mRNAs were not increased by phorbol ester treatment alone. Then, we demonstrated that the mRNA and proteins of the IFN-gamma receptor alpha- and beta-chains were amplified by phorbol ester treatment in THP-1 cells. Consequently, these results indicate that the enhancement of DR alpha gene expression by IFN-gamma treatment in phorbol ester-activated THP-1 cells is due to the phorbol ester-induced up-regulation of IFN-gamma receptor alpha- and beta-chains. As a result, the amplification of STAT1 alpha and the increment of class II MHC trans-activator results in enhancement of DR alpha expression.
Sakamoto
S
S
Laboratory of Molecular Biology, Medical Research Center, Kochi Medical School, Japan.
Nie
J
J
Taniguchi
T
T
D002460:Cell Line; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D005802:Genes, MHC Class II / Q000187:drug effects; D006684:HLA-DR Antigens / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism* / Q000494:pharmacology; D009687:Nuclear Proteins; D012333:RNA, Messenger / Q000096:biosynthesis; D017471:Receptors, Interferon / Q000096:biosynthesis*; D050794:STAT1 Transcription Factor; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics / Q000502:physiology; D000096743:Interferon gamma Receptor
D004268:DNA-Binding Proteins; D006684:HLA-DR Antigens; D007371:Interferon-gamma; D009687:Nuclear Proteins; D012333:RNA, Messenger; D017471:Receptors, Interferon; D050794:STAT1 Transcription Factor; D013755:Tetradecanoylphorbol Acetate; D015534:Trans-Activators; D000096743:Interferon gamma Receptor; C083728:MHC class II transactivator protein; C494079:STAT1 protein, human