A novel strategy affords high-yield coupling of antibody to extremities of liposomal surface-grafted PEG chains.
Several methodologies for the preparation of polyethylene glycol-grafted immunoliposomes have been developed by attaching antibodies to the terminus of the polymer. Unilamellar liposomes were prepared containing a combination of a functionalized polyethylene glycol(3400) and an inert polyethylene glycol(2000) phosphatidylethanolamine derivate up to 5 mol%. The greater length of the functionalized polyethylene glycol derivate did not alter the liposomal sterical stability or the remote loading of doxorubicin. Anti-CD34 immunoliposomes were prepared by the reaction of maleimide-derivatized My10 antibody with generated thiol groups at the periphery of the liposomes and efficiencies of nearly 100% were obtained. The greater accessibility of the reactive group makes this strategy more efficient than others described. The immunoliposomes prepared bound specifically to CD34+ cells.
Mercadal
M
M
Department of Biochemistry and Molecular Biology, Faculty of Chemistry, University of Barcelona, Martí i Franqués, 1, 08028, Barcelona, Spain.
Domingo
J C
JC
Petriz
J
J
Garcia
J
J
de Madariaga
M A
MA
D000911:Antibodies, Monoclonal / Q000737:chemistry* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D018952:Antigens, CD34 / Q000276:immunology; D004337:Drug Carriers; D004355:Drug Stability; D008081:Liposomes / Q000138:chemical synthesis*; D011092:Polyethylene Glycols / Q000737:chemistry*
D000911:Antibodies, Monoclonal; D018952:Antigens, CD34; D004337:Drug Carriers; D008081:Liposomes; D011092:Polyethylene Glycols