Phagocytosis of new cementum-like islets formed inside the gingival connective tissue in cyclosporin-A treated rats.
Three control and 3 experimental rats were administered vehicle or cyclosporin-A solutions. Animals were anaesthetized, tissues fixed and jaws processed for Epon inclusion. Histological examination of serially cut areas revealed the presence inside the gingival connective tissue of new cementum-like islets (NCLIs) associated to or engulfed by voluminous multinucleated cells (MCs). These complexes were located adjacent to blood vessels, at 250-350 microns from the root surface. Histomorphometric study indicated that the volume of the NCLIs varied from 3900 to 72,900 microns 3 and that of the MCs from 822 to 56,190 microns 3. The latter bore up to 14 nuclear profiles. Comparative evaluation of the NCLI-MC associations to other complexes "multinucleated cell(s)-resorbed material" seems to indicate that the phagocytosis of the NCLIs is dictated by their ectopic location, rather than their nature (new cementum-like structures). Therefore the MCs should be considered as foreign body giant multinucleated cells rather than cementoclasts.
Ayanoglou
C M
CM
Laboratoire Biologie et Biomatériaux du Milieu Buccal et Osseux, Faculté Chirurgie Dentaire, Université Paris V, Montrouge, France.
D000818:Animals; D003238:Connective Tissue / Q000098:blood supply / Q000276:immunology / Q000473:pathology; D016572:Cyclosporine / Q000633:toxicity*; D003739:Dental Cementum / Q000473:pathology*; D015743:Giant Cells, Foreign-Body / Q000276:immunology; D005881:Gingiva / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D002479:Inclusion Bodies / Q000473:pathology; D008297:Male; D010587:Phagocytosis; D051381:Rats; D017207:Rats, Sprague-Dawley
D016572:Cyclosporine