MHC-restricted, glycopeptide-specific T cells show specificity for both carbohydrate and peptide residues.
We examined the antigenic specificity of two T cell hybridomas elicited against the disaccharide galabiose attached to the fifth residue of the I-Ak binding peptide 52-61 of lysozyme. By making changes in the saccharide molecule and in the peptide, we conclude that the outer galactose residue of the galabiose moiety is directly recognized by the T cells together with the exposed side chains of the peptide. The overall spatial display of this galactose moiety on the 52-61 peptide is likewise important.
Deck
M B
MB
Organic Chemistry, Umeå University, Sweden.
Sjölin
P
P
Unanue
E R
ER
Kihlberg
J
J
D000596:Amino Acids / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D004187:Disaccharides / Q000276:immunology* / Q000378:metabolism; D018984:Epitopes, T-Lymphocyte / Q000378:metabolism*; D005998:Glycine / Q000276:immunology / Q000378:metabolism; D006020:Glycopeptides / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism*; D006825:Hybridomas; D051379:Mice; D008808:Mice, Inbred CBA; D009113:Muramidase / Q000276:immunology / Q000378:metabolism; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism; D012694:Serine / Q000276:immunology / Q000378:metabolism
D000596:Amino Acids; D004187:Disaccharides; D018984:Epitopes, T-Lymphocyte; D006020:Glycopeptides; D000949:Histocompatibility Antigens Class II; D010446:Peptide Fragments; D011948:Receptors, Antigen, T-Cell; C032103:4-O-alpha-D-galactopyranosyl-D-galactose; D012694:Serine; D009113:Muramidase; D005998:Glycine