Transformation of cortisone acetate using immobilized Arthrobacter simplex cells.
A new cell immobilization method was developed in which cells of Arthrobacter simplex BY-2-3-51 were immobilized by entrapment with a polyvinyl alcohol-alginate complex carrier. The immobilized cells preparation contains spherical beads with high mechanical strength and delta 1-dehydrogenase activity. The activation conditions and enzymatic properties of the immobilized cells were studied. When the initial substrate concentration was 20 mg/mL, the conversion of cortisone acetate to prednisone acetate by immobilized cells in the shaken culture was 98% at 18 hours. The stability of immobilized cells was maintained by activation at proper times.
Zhang
Y
Y
Department of Chemical Engineering, Tianjin University, China.
Yao
C
C
Wang
M
M
Lu
Y
Y
Wang
Z
Z
D001173:Arthrobacter / Q000378:metabolism*; D003348:Cortisone / Q000031:analogs & derivatives* / Q000378:metabolism
D003348:Cortisone