A gene encoding a hevein-like protein from elderberry fruits is homologous to PR-4 and class V chitinase genes.
We isolated SN-HLPf (Sambucus nigra hevein-like fruit protein), a hevein-like chitin-binding protein, from mature elderberry fruits. Cloning of the corresponding gene demonstrated that SN-HLPf is synthesized as a chimeric precursor consisting of an N-terminal chitin-binding domain corresponding to the mature elderberry protein and an unrelated C-terminal domain. Sequence comparisons indicated that the N-terminal domain of this precursor has high sequence similarity with the N-terminal domain of class I PR-4 (pathogenesis-related) proteins, whereas the C terminus is most closely related to that of class V chitinases. On the basis of these sequence homologies the gene encoding SN-HLPf can be considered a hybrid between a PR-4 and a class V chitinase gene.
Van Damme
E J
EJ
Laboratory for Phytopathology and Plant Protection, Willem de Croylaan 42, Katholieke Universiteit Leuven, 3001 Leuven, Belgium. els.vandamme@agr.kuleuven.ac.be
Charels
D
D
Roy
S
S
Tierens
K
K
Barre
A
A
Martins
J C
JC
Rougé
P
P
Van Leuven
F
F
Does
M
M
Peumans
W J
WJ
D000595:Amino Acid Sequence; D023181:Antimicrobial Cationic Peptides; D001483:Base Sequence; D002688:Chitinases / Q000737:chemistry / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D017931:DNA Primers / Q000235:genetics; D005638:Fruit / Q000201:enzymology* / Q000235:genetics*; D017343:Genes, Plant; D007438:Introns; D037102:Lectins / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D037121:Plant Lectins; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011487:Protein Conformation; D017386:Sequence Homology, Amino Acid
D023181:Antimicrobial Cationic Peptides; D017931:DNA Primers; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; C045875:hevein; D002688:Chitinases