Differential import of nuclear-encoded tRNAGly isoacceptors into solanum Tuberosum mitochondria.
In potato ( Solanum tuberosum ) mitochondria, about two-thirds of the tRNAs are encoded by the mitochondrial genome and one-third is imported from the cytosol. In the case of tRNAGly isoacceptors, a mitochondrial-encoded tRNAGly(GCC) was found in potato mitochondria, but this is likely to be insufficient to decode the four GGN glycine codons. In this work, we identified a cytosolic tRNAGly(UCC), which was found to be present in S.tuberosum mitochondria. The cytosolic tRNAGly(CCC) was also present in mitochondria, but to a lesser extent. By contrast, the cytosolic tRNAGly(GCC) could not be detected in mitochondria. This selective import of tRNAGly isoacceptors into S. tuberosum mitochondria raises further questions about the mechanism under-lying the specificity of the import process.
Brubacher-Kauffmann
S
S
Institut de Biologie Moléculaire des Plantes du CNRS (UPR 0406), Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France.
Maréchal-Drouard
L
L
Cosset
A
A
Dietrich
A
A
Duchêne
A M
AM
D000926:Anticodon; D001483:Base Sequence; D001692:Biological Transport; D015152:Blotting, Northern; D002467:Cell Nucleus / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D015347:RNA Probes; D012353:RNA, Transfer, Gly / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011198:Solanum tuberosum / Q000378:metabolism* / Q000648:ultrastructure
D000926:Anticodon; D015347:RNA Probes; D012353:RNA, Transfer, Gly