Effect of 2,2'-dipyridyl on accumulation of protoporphyrin IX and its derivatives in yeast mitochondria and plasma membranes.
The iron chelator 2,2'-dipyridyl (0.2 mM) more than fourfold increased the concentration of protoporphyrin IX and also of its zinc-containing complex in mitochondria of the yeast Saccharomyces cerevisiae. Protoporphyrin IX and a chlorine derivative of protoporphyrin IX which fluoresces at 670-675 nm were found in isolated plasma membranes of the yeast grown in the presence of 0.2 mM 2,2'-dipyridyl. The accumulation of endogenous porphyrins resulted in intensification of lipid photoperoxidation in mitochondria and plasma membranes and in a dramatically increased sensitivity of the cells to visible light (400-600 nm). The relative contribution of photodestruction of subcellular structures to photoinduced cell inactivation is discussed.
Strakhovskaya
M G
MG
Department of Biophysics, School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia.
Ivanova
E V
EV
Kolesnikova
O A
OA
Fraikin
G Y
GY
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000494:pharmacology*; D000622:Aminolevulinic Acid / Q000494:pharmacology; D002462:Cell Membrane / Q000187:drug effects*; D007502:Iron Chelating Agents / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects*; D017319:Photosensitizing Agents / Q000494:pharmacology; D011524:Protoporphyrins / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D007502:Iron Chelating Agents; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D015082:2,2'-Dipyridyl; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX