Chiral high-performance liquid chromatographic analysis of enantiomers of losigamone, a new candidate antiepileptic drug.
An assay based on a single-step liquid-liquid extraction from human plasma followed by high-performance liquid chromatography on a chiral column was developed for the measurement of enantiomers of a racemic new candidate antiepileptic drug. Excellent intra- and inter-assay accuracy and precision and recovery were demonstrated in the desired concentration range of 0.031 to 5.00 microg/ml. The method is free from interferences by other anticonvulsant drugs and their metabolites. The method is being used in a clinical trial of losigamone.
Torchin
C D
CD
Epilepsy Therapeutics Research Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA.
Yonekawa
W D
WD
Kapetanovic
I M
IM
Kupferberg
H J
HJ
D000927:Anticonvulsants / Q000097:blood* / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005663:Furans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013237:Stereoisomerism
D000927:Anticonvulsants; D005663:Furans; C065427:losigame