Effect of alpha-tocopherol pretreatment on high energy metabolites in rabbit skeletal muscle after ischemia-reperfusion.
The ability of skeletal muscle to recover high energy phosphate compounds in response to pretreatment with vitamin E was investigated in a rabbit hindlimb ischemia/reperfusion model (2. 5 h/2 h). High energy metabolites were measured in the adductor magnus muscle of untreated animals and compared to the treatment group (all rac-alpha-tocopheryl acetate, 3 mg/kg body weight, supplemented i.v. before the onset of ischemia). Phosphocreatine (PCr) levels decreased after ischemia more than 65% in untreated and treatment groups, but tended to recover in treatment group after reperfusion. Adenosine triphosphate (ATP) values decreased by 50% of basal level after reperfusion in the untreated group, whereas alpha-tocopherol pretreatment prevented ATP depletion.
Punz
A
A
AP, Department of Surgery, Surgical Research Laboratories, AKH, Währinger Gürte 18-20 Vienna A-1090 Austria.
Nanobashvili
J
J
Fuegl
A
A
Huk
I
I
Roth
E
E
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects*; D006614:Hindlimb; D007511:Ischemia / Q000378:metabolism*; D008297:Male; D018482:Muscle, Skeletal / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D010725:Phosphocreatine / Q000378:metabolism; D011817:Rabbits; D015424:Reperfusion; D024505:Tocopherols; D014810:Vitamin E / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D024502:alpha-Tocopherol / Q000031:analogs & derivatives*
D010725:Phosphocreatine; D014810:Vitamin E; D000255:Adenosine Triphosphate; D024502:alpha-Tocopherol; D024505:Tocopherols