Vitrification of two-cell rat embryos derived from immature hypothyroid rdw rats by in vitro fertilization in ethylene glycol-based solutions.
Two-cell embryos derived from immature rdw rats by in vitro fertilization (IVF) were vitrified in ethylene glycol-based solutions. Embryos exposed to EFS20 before being vitrified in EFS40 exhibited improved viability in vitro. All embryos exposed to EFS20 for 1-3 min before vitrification in EFS40 were morphologically normal. However, 2-3 min of exposure to EFS20 increased the number of embryos that developed beyond the four-cell stage. More embryos exposed to EFS20 for 2-3 min developed to morulae (63-64%) and blastocysts (34-38%) than those exposed for 1 min (35 and 10%, respectively). After transfer, 33% of embryos exposed to EFS20 for 3 min and vitrified in EFS40 developed to term compared to 29% of fresh embryos. Fifteen (47%) of live young were homozygous rdw and all of the others were heterozygous rats. The present study demonstrated that vitrification in EFS solution can be routinely used to cryopreserve rat two-cell IVF-embryos with no loss of viability.
Jiang
J Y
JY
Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, Japan. jiang@bios.tohoku.ac.jp
Umezu
M
M
Sato
E
E
D000818:Animals; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents; D004392:Dwarfism / Q000235:genetics; D004624:Embryo Transfer; D004622:Embryo, Mammalian; D005314:Embryonic and Fetal Development; D019855:Ethylene Glycol; D005260:Female; D005307:Fertilization in Vitro; D007037:Hypothyroidism / Q000235:genetics*; D008297:Male; D011247:Pregnancy; D051381:Rats; D011922:Rats, Mutant Strains
D003451:Cryoprotective Agents; D019855:Ethylene Glycol