Plasma total homocysteine measurement by ion-paired reversed-phase HPLC with electrochemical detection.
Homocysteine is thought to be a risk factor for vascular disease and this has led to an increase in demand for its assay in clinical laboratories. An HPLC method, incorporating electrochemical detection, for measurement of plasma total homocysteine is presented. The method is simple to perform, precise and suitable for clinical applications.
Martin
S C
SC
Department of Clinical Biochemistry, Heartlands Hospital, Birmingham, UK.
Hilton
A C
AC
Bartlett
W A
WA
Jones
A F
AF
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results
D006710:Homocysteine