Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines.
We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate vaccine construction. The selected polysaccharide fragments can be used to produce glycoconjugate vaccines containing a restricted saccharide polydispersion. We have applied this chromatographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The method is easily adapted for manufacturing purposes.
Costantino
P
P
Chiron Vaccines SpA, Siena, Italy.
Norelli
F
F
Giannozzi
A
A
D'Ascenzi
S
S
Bartoloni
A
A
Kaur
S
S
Tang
D
D
Seid
R
R
Viti
S
S
Paffetti
R
R
Bigio
M
M
Pennatini
C
C
Averani
G
G
Guarnieri
V
V
Gallo
E
E
Ravenscroft
N
N
Lazzeroni
C
C
Rappuoli
R
R
Ceccarini
C
C
D000942:Antigens, Bacterial / Q000737:chemistry*; D016667:Bacterial Capsules / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D004797:Enzyme-Linked Immunosorbent Assay; D018073:Haemophilus Vaccines / Q000737:chemistry*; D006868:Hydrolysis; D013058:Mass Spectrometry; D009345:Neisseria meningitidis; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D014462:Ultrafiltration; D018074:Vaccines, Conjugate / Q000737:chemistry*
D000942:Antigens, Bacterial; D018073:Haemophilus Vaccines; C044237:Haemophilus influenzae type b polysaccharide vaccine; D011135:Polysaccharides, Bacterial; D018074:Vaccines, Conjugate; C037676:meningococcal group C polysaccharide