The effect of combined administration of cadmium and furosemide on auditory function in the rat.
A number of heavy metals have been associated with toxic effects to the peripheral or central auditory system. These include lead, arsenic, mercury, platinum and organic tin compounds. In addition, the ototoxic effects of some metals may be potentiated by other factors. However, the auditory effects of cadmium have not previously been reported. The purpose of the present study was to investigate the potential ototoxic effects of cadmium from an acute dosage, and its potentiation by furosemide. Auditory brainstem response (ABR) thresholds were measured in adult Sprague-Dawley rats. Rats received either cadmium chloride (5 mg/kg, i.p.) followed by saline (4 ml/kg, i.p.). cadmium chloride followed by furosemide (200 mg/kg, i.p.), or furosemide alone. Follow-up ABRs were carried out 7 days post-treatment and threshold changes were compared between each treatment group. No significant threshold change was seen for the cadmium chloride plus saline treated or the furosemide treated animals. However, significant threshold elevations were observed in animals receiving cadmium chloride plus furosemide. In addition, scanning electron microscopy revealed extensive hair cell loss in animals treated with cadmium chloride and furosemide. Although functional auditory changes were not seen after the administration of cadmium alone, the potentiation of threshold changes by furosemide suggests that cadmium may be ototoxic under certain conditions.
Whitworth
C A
CA
Southern Illinois University School of Medicine, Department of Surgery, Springfield 62794-1221, USA.
Hudson
T E
TE
Rybak
L P
LP
D000818:Animals; D001309:Auditory Threshold / Q000187:drug effects; D019256:Cadmium Chloride / Q000008:administration & dosage* / Q000633:toxicity*; D004232:Diuretics / Q000008:administration & dosage* / Q000633:toxicity*; D004357:Drug Synergism; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects; D005665:Furosemide / Q000008:administration & dosage* / Q000633:toxicity*; D006198:Hair Cells, Auditory / Q000187:drug effects / Q000293:injuries / Q000648:ultrastructure; D006309:Hearing / Q000187:drug effects*; D008297:Male; D008855:Microscopy, Electron, Scanning; D009925:Organ of Corti / Q000187:drug effects / Q000293:injuries / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley
D004232:Diuretics; D005665:Furosemide; D019256:Cadmium Chloride