[Strain differentiation of the Newcastle disease virus by reverse transcriptase-polymerase chain reaction and sequencing population exchange].
A system for detection and strain differentiation of Newcastle disease virus (NDV) by reverse transcription of polymerase chain reaction (RT-PCR) (isolation of RNA, choice of primers for nested PCR, and purification of PCR products) and sequencing is developed and optimized. A nucleotide sequence of gene F site, coding for the F2/F1 cleavage site of F0 fusion protein and including several hypervariable regions, is determined for 10 Russian strains and vaccine strains. The data indicate a replacement of NDV populations in Russia and a rapid evolution of the virus. The origin of pathogenic NDV strains which have been circulating up to the present time is still unknown.
Gribanov
O G
OG
Perevozchikova
N A
NA
Starov
S K
SK
Smolenski
V I
VI
Rudenko
T V
TV
Drygin
V V
VV
Gusev
A A
AA
D001483:Base Sequence; D004269:DNA, Bacterial; D008969:Molecular Sequence Data; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity
D004269:DNA, Bacterial