title: Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa.
abstract: Guinea-pig spermatozoa in the presence of a purified fraction from Echeveria gibbiflora aqueous crude extract suffer a hypotonic-like effect. The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge 'head-bubble'. The agglutination effect was so enhanced that instead of inducing sperm clusters, it produced cane-like 'stalk' structures. The immobilizing activity was induced instantaneously after the addition of the purified fraction. At electron microscope level it was possible to observe a heavy amount of electron dense material of the purified fraction embedded or intercalated along the plasma membrane. It was also possible to corroborate the dispersion of the acrosomal content and the disappearance of the external acrosome membrane. The purified fraction induced loosening of the plasma membrane all along the sperm cell, however, the distension of the membrane was only produced in the apical portion of the sperm head and not in the post equatorial region. The results suggest that the plant may yield a compound suitable for use as a vaginal barrier or male contraceptive agent.
authors: División de Biología de la Reproducción, Centro de Investigación Biomédica del Sur, IMSS, Morelos, Mexico.
authors: N M
authors: NM
authors: Delgado
authors: J
authors: J
authors: Taboada Ramírez
authors: A
authors: A
authors: Ortega Hernández
authors: H
authors: H
authors: Merchant-Larios
authors: M L
authors: ML
authors: Sánchez-Vázqueź
authors: G
authors: G
authors: Ramírez
authors: R
authors: R
authors: Reyes
mesh_terms: D000177:Acrosome / Q000187:drug effects / Q000648:ultrastructure; D000818:Animals; D001681:Biological Assay; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008800:Mexico; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000648:ultrastructure
chemical_list: D010936:Plant Extracts