Chiral discrimination using an immunosensor.
Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatized alpha-amino acids can be monitored in a competitive assay by their interaction with antibodies specific for the chiral center of this class of substances. The sensitivity to the minor enantiomer in nonracemic mixtures exceeds currently available methods; therefore, such immunosensors can readily detect traces of enantiomeric impurities and are attractive for a range of applications in science and industry.
Hofstetter
O
O
Institute of Organic Chemistry, University of Tübingen, Germany.
Hofstetter
H
H
Wilchek
M
M
Schurig
V
V
Green
B S
BS
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000737:chemistry / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D007136:Immunoglobulins / Q000737:chemistry / Q000276:immunology; D011817:Rabbits; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D020349:Surface Plasmon Resonance / Q000379:methods*
D000596:Amino Acids; D000906:Antibodies; D007136:Immunoglobulins