title: Cl- channels in basolateral TAL membranes. XIV. Kinetic properties of a basolateral MTAL Cl- channel.
abstract: BACKGROUND
  This article reports studies on the kinetics of chloride (Cl-) conductance in Cl- channels fused into bilayers from basolaterally enriched vesicles from rabbit outer medulla. A considerable body of evidence indicates that these channels represent rbClC-Ka, a 77 kDa kidney-specific protein of the ClC family of Cl- channels. rbClC-Ka, a candidate channel for mediating net Cl- absorption in the medullary thick ascending limb (MTAL), has been cloned from rabbit outer medulla and localized by immunofluorescence to basolateral membranes of the MTAL. Thus, this is the first account, to our knowledge, of the kinetics of ion permeation through a renal Cl- channel mediating net basolateral Cl- absorption in the thick ascending limb of Henle (TALH), and this channel may represent rbClC-Ka.
  
  
  METHODS
  The electrophysiological properties of these channels were studied by fusing basolaterally enriched MTAL vesicles into planar bilayer membranes.
  
  
  RESULTS
  Cl- conductance through these channels was concentration dependent and saturable. The relationship between gCl (pS) and symmetrical aqueous Cl- concentrations could be expressed in terms of the Michaelis equation with a limiting conductance (GClmax, pS) of 114 pS at infinitely high aqueous Cl- concentrations and a K1/2 of 163 mM Cl-. A log-log plot of the conductance-Cl- concentration relations, in the nonsaturating Cl- concentration range, had a slope of 0.91, that is, virtually unity. The relatively impermeant anion I- produced a voltage-dependent conductance blockade that could be overcome at high electric field strengths.
  
  
  CONCLUSIONS
  The experimental data described earlier here fulfill the traditional criteria for a first-order process with a single Cl- ion occupying these channels at a given time. Although the channels may contain multiple ion binding sites, the latter function, in integral kinetic terms, as a single rate-limiting locus.
authors: Department of Internal Medicine, University of Arkansas College of Medicine, Little Rock, USA.
authors: C J
authors: CJ
authors: Winters
authors: W B
authors: WB
authors: Reeves
authors: T E
authors: TE
authors: Andreoli
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000737:chemistry; D018118:Chloride Channels / Q000737:chemistry / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism*; D066298:In Vitro Techniques; D007679:Kidney Medulla / Q000737:chemistry; D007700:Kinetics; D016014:Linear Models; D008051:Lipid Bilayers / Q000737:chemistry; D018408:Patch-Clamp Techniques; D011817:Rabbits
chemical_list: D018118:Chloride Channels; D002712:Chlorides; D008051:Lipid Bilayers