title: Atopy patch test reactions are associated with T lymphocyte-mediated allergen-specific immune responses in atopic dermatitis.
abstract: BACKGROUND
  Positive test reactions to epicutaneous application of aeroallergens served as a model of 'early' eczema in atopic dermatitis (AD) in a number of dermato-immunological studies. However, no quantitative evaluation has been performed so far comparing specific T-cell activation parameters in the peripheral blood of AD patients with positive or negative atopy patch tests (APT).
  
  
  OBJECTIVE
  The purpose of this study was to investigate specific immunological parameters in patients with atopic dermatitis showing positive or negative atopy patch tests reactions.
  
  
  METHODS
  APT results (n = 96) were compared with allergen-specific IgE, specific lymphocyte proliferation, and the expression of 'activation' markers on peripheral blood T-cells upon in vitro stimulation with house dust mite, cat or grass pollen allergens.
  
  
  RESULTS
  Only a subpopulation (48%) of patients sensitized to aeroallergens (i.e. specific IgE > 0.7 kU/L) developed APT-reactions to the corresponding allergen. APT reactions were, however, significantly associated with allergen specific lymphocyte proliferation (p < 0.0001), and a higher number of CD54+ or CD30+ T-cells (p < 0.05) upon in vitro stimulation.
  
  
  CONCLUSIONS
  The association of delayed skin reactions with allergen specific T-cell parameters in the blood points to an immunologically mediated mechanism leading to positive reactions in the APT.
authors: Department of Dermatology and Allergology, Hannover Medical University, Hannover, Germany.
authors: A
authors: A
authors: Wistokat-Wülfing
authors: P
authors: P
authors: Schmidt
authors: U
authors: U
authors: Darsow
authors: J
authors: J
authors: Ring
authors: A
authors: A
authors: Kapp
authors: T
authors: T
authors: Werfel
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D003876:Dermatitis, Atopic / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D008213:Lymphocyte Activation; D008925:Mites / Q000276:immunology; D010328:Patch Tests; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000485:Allergens