Preservation of some extremely thermophilic chemolithoautotrophic bacteria by deep-freezing and liquid-drying methods.
Long-term preservation methods for extreme thermophilic chemolithoautotrophic bacteria representing various species are described. The cultures were cryopreserved in liquid nitrogen under anaerobic conditions using 5% dimethylsulfoxide as a cryoprotectant. For easy storage and transport, the cultures were successfully liquid-dried, directly from the liquid phase without involving freezing under semiaerobic conditions using effective protective agents such as ethylenediamine and meso-inositol. The tested cultures showed good stability and survival rates after drying, after cryopreservation and on long-term storage. All tested strains were successfully preserved and reactivated within relatively short time. The viability, stability and ability of chemolithoautotrophic growth was not affected. Cryopreservation, liquid-drying and reactivation under microaerobic conditions proved very effective for these oxygen sensitive cultures.
Malik
K A
KA
DSMZ-Deutsche Saumlung von Nikroorganismen und Zeilkulturen, GmbH, Nascheroder Weg 1b, Braunschweig, Germany.
Halik
K A
KA
D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D015925:Cryopreservation; D006091:Gram-Negative Chemolithotrophic Bacteria / Q000254:growth & development / Q000502:physiology*