Chloroplast NADH dehydrogenase from Pisum sativum: characterization of its activity and cloning of ndhK gene.
The pea chloroplast ndhK gene coding for a component of a NADH-plastoquinone oxidoreductase has been cloned and sequenced. This gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 Da which belongs to the family of the 20 kDa PSST subunit of the bovine mitochondrial complex I. A fragment of this gene has been overexpressed in Escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoid membranes. This polypeptide is a component of a protein complex with NADH dehydrogenase activity and is not associated with ferredoxin-NADP+ reductase.
Elortza
F
F
Biokimika eta Biologia Molekularreko Saila, Euskal Herriko Unibertsitatea, Bilbao, Spain.
Asturias
J A
JA
Arizmendi
J M
JM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002736:Chloroplasts / Q000201:enzymology; D003001:Cloning, Molecular; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics*; D009250:NADP Transhydrogenases / Q000235:genetics* / Q000378:metabolism; D018532:Pisum sativum / Q000201:enzymology* / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D009250:NADP Transhydrogenases; D009245:NADH Dehydrogenase