title: Thromboembolic events predispose the brain to widespread cerebral infarction after delayed transient global ischemia in rats.
abstract: OBJECTIVE
  Transient distal platelet accumulation after common carotid artery thrombosis (CCAT) leads to hemodynamic, metabolic, and molecular events that may influence the response of the postthrombotic brain to secondary insults. We investigated how a thromboembolic insult would affect histopathological outcome when combined with an ischemic insult induced 24 hours later.
  
  
  METHODS
  Three groups of rats underwent either (1) CCAT+10 minutes of normothermic 2-vessel occlusion (n=6), (2) CCAT+sham ischemia procedures (n=6), or (3) sham CCAT procedures+10 minutes of 2-vessel occlusion (n=6). At 7 days, rats were perfused for quantitative histopathological and immunocytochemical analysis.
  
  
  RESULTS
  Rats undergoing combined insults (group 1) had significantly larger areas of ischemic injury (P<0.05) within the cerebral cortex, striatum, and thalamus compared with the other, single-injury groups. Increased ischemic damage included selective neuronal necrosis, infarction, and focal hemorrhage. By means of glial fibrillary acidic protein immunocytochemistry and lectin histochemistry, reactive astrocytes and microglia were found to be associated with widespread tissue necrosis. In contrast, infrequent infarction or CA1 hippocampal neuronal necrosis was observed in groups 2 and 3, respectively.
  
  
  CONCLUSIONS
  A prior thromboembolic event is a risk factor for widespread cerebral infarction and hemorrhage when combined with a delayed ischemic insult. The understanding of what factors enhance the susceptibility of the postthrombotic brain to secondary insults may aid in the development of neuroprotective strategies to be applied after transient ischemic attacks to prevent the initiation of stroke.
authors: Department of Neurological Surgery, University of Miami School of Medicine, Fla.33101, USA. dalton@stroke.med.miami.edu
authors: W D
authors: WD
authors: Dietrich
authors: G
authors: G
authors: Danton
authors: A C
authors: AC
authors: Hopkins
authors: R
authors: R
authors: Prado
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000737:chemistry / Q000502:physiology; D001792:Blood Platelets / Q000502:physiology; D017536:Carotid Artery, Common; D002452:Cell Count; D002540:Cerebral Cortex / Q000098:blood supply / Q000166:cytology; D002544:Cerebral Infarction / Q000453:epidemiology / Q000209:etiology / Q000503:physiopathology*; D003342:Corpus Striatum / Q000098:blood supply / Q000166:cytology; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis; D006624:Hippocampus / Q000098:blood supply / Q000166:cytology; D002546:Ischemic Attack, Transient / Q000150:complications / Q000453:epidemiology / Q000503:physiopathology*; D008297:Male; D017628:Microglia / Q000737:chemistry / Q000502:physiology; D009474:Neurons / Q000166:cytology; D051381:Rats; D017208:Rats, Wistar; D012307:Risk Factors; D013923:Thromboembolism / Q000150:complications / Q000453:epidemiology / Q000503:physiopathology*
chemical_list: D005904:Glial Fibrillary Acidic Protein