Antigen-induced elevation of immunoreactive endothelin-1 (ET-1) levels in ovalbumin-sensitized guinea pig airway tissue.
Changes in the immunoreactive ET-1 levels during the anaphylactic reaction of airway tissue from ovalbumin-sensitized guinea pigs were investigated. ET-1-immunoreactivity (ET-IR) was detected in the epithelial and smooth muscle layers of tracheal sections from normal guinea pigs and it was enhanced slightly by phosphoramidon (1 microM) treatment. The ET-IR level of the epithelial layer of ovalbumin-treated tissue from actively sensitized animals was slightly higher than that from normal animals, but it was enhanced markedly by phosphoramidon (1 microM) treatment. Furthermore, the mean ET-IR level of homogenates of antigen-treated tracheal tissues from sensitized guinea pigs (22.8 +/- 1.55 fmol mg-1 protein, n = 5) was significantly higher than the corresponding normal level (12.3 +/- 1.21 fmol mg-1 protein, n = 5). These results suggest that increased epithelial airway ET-1 levels contribute to the anaphylactic reaction of guinea pig airways.
Kizawa
Y
Y
Department of Physiology and Anatomy, Nihon University College of Pharmacy, Funabashi, Chiba, Japan.
Kotake
H
H
Kusama
T
T
Saito
K
K
Murakami
H
H
D000707:Anaphylaxis / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D019332:Endothelin-1 / Q000032:analysis / Q000378:metabolism*; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D006020:Glycopeptides / Q000494:pharmacology; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D009130:Muscle, Smooth / Q000276:immunology / Q000378:metabolism; D010047:Ovalbumin / Q000276:immunology*; D014132:Trachea / Q000276:immunology / Q000378:metabolism*
D000941:Antigens; D019332:Endothelin-1; D006020:Glycopeptides; D010047:Ovalbumin; C008890:phosphoramidon