Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.).
We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and SOD assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.
Kaminaka
H
H
Laboratory of Genetic Engineering, Faculty of Agriculture, Kyoto Prefectural University, Japan.
Morita
S
S
Tokumoto
M
M
Yokoyama
H
H
Masumura
T
T
Tanaka
K
K
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D018506:Gene Expression Regulation, Plant; D007202:Indicators and Reagents / Q000032:analysis; D007501:Iron / Q000032:analysis; D008345:Manganese / Q000032:analysis; D008969:Molecular Sequence Data; D009580:Nitroblue Tetrazolium / Q000032:analysis; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000737:chemistry; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000737:chemistry / Q000235:genetics*
D018744:DNA, Plant; D007202:Indicators and Reagents; D011994:Recombinant Proteins; D009580:Nitroblue Tetrazolium; D008345:Manganese; D007501:Iron; D013482:Superoxide Dismutase