Platelet-activating factor inhibits ciliary beat frequency of human bronchial epithelial cells.
The aim of our study was to investigate the effect of platelet-activating factor on human bronchial ciliary beat frequency (CBF) ex vivo. Brush biopsies were obtained from eight children (mean age 6.6 years) who underwent bronchoscopy for clinical reasons (i.e., foreign body aspiration, chronic cough, stridor). Immediate measurement of CBF by phase-contrast microscopy and photoelectric technique was performed before further treatment, and then on three subsamples: after adding platelet-activating factor (PAF) (10(-5) M); after adding the inhibitor WEB 2086 (10(-4) M); and after adding both PAF and WEB 2086. Addition of 10(-5) M PAF reduced mean CBF by 2.1 Hz to 9.2 Hz (p < 0.05). The inhibitory effect of PAF was completely reversed by the addition of the PAF-inhibitor WEB 2086, whereas WEB 2086 alone did not significantly influence CBF. Optimum PAF concentration had been determined in previous dose-response experiments. Our data indicates that PAF involved in airway inflammation contributes to a decrease in mucociliary clearance in humans.
Klettke
U
U
Division of Pediatric Pneumology and Immunology, Charité, Humboldt University, Berlin, Germany.
Luck
W
W
Wahn
U
U
Niggemann
B
B
D000293:Adolescent; D001980:Bronchi / Q000166:cytology*; D002648:Child; D002675:Child, Preschool; D002923:Cilia / Q000187:drug effects / Q000502:physiology*; D004847:Epithelial Cells / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D010972:Platelet Activating Factor / Q000494:pharmacology*; D013997:Time Factors
D010972:Platelet Activating Factor