Isolation and characterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase.
A cDNA clone encoding 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) was isolated from a human fetal liver cDNA library. It contained the open reading frame of 2,709 base pairs and predicted a protein comprising 902 amino acids with a calculated molecular weight of 98,700 Da. The deduced protein showed about 93.6% homology (90.5% identity, 3.1% favored substitutions) when compared with rat 10-FTHFDH. The distribution of 10-FTHFDH transcript in various human tissues was studied by Northern blot analysis using poly(A+) RNAs from different tissues. The 10-FTHFDH transcript with an approximate size of 2.7 kb was mainly expressed in human kidney, skeletal muscle, and liver and rarely expressed in other tissues.
Hong
M
M
Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
Lee
Y
Y
Kim
J W
JW
Lim
J S
JS
Chang
S Y
SY
Lee
K S
KS
Paik
S G
SG
Choe
I S
IS
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000099295:Formyltetrahydrofolate Dehydrogenase
D018076:DNA, Complementary; D000587:Oxidoreductases Acting on CH-NH Group Donors; D012333:RNA, Messenger; D000099295:Formyltetrahydrofolate Dehydrogenase