Modification of antinociceptive action of Ukrain by endogenous nitric oxide in the writhing syndrome test in mice.
The effects of L-arginine, the physiological precursor of nitric oxide (NO), and inhibitors of NO-synthase on the antinociceptive action of Ukrain (4.75, 9.5, and 19.0 mg/kg i.p.) were investigated using the writhing syndrome test in mice. It was found that L-arginine (0.1 or 1.0 mg/kg i.p.) significantly decreased or enhanced the antinociceptive effect of Ukrain, depending on the combination administered. In addition, the inhibitors of NO-synthase NG-nitro-L-arginine methyl ester (L-NAME) (1.0 and 10 mg/kg i.p.), 7-nitroindazole (1.0 mg/kg i.p.) and NG-monomethyl-L-arginine acetate (L-NMMA) (1.0 mg/kg i.p.) significantly enhanced Ukrain-induced antinociception. These results suggest that endogenous NO can modify the antinociceptive effect of Ukrain.
Jagiello-Wójtowicz
E
E
Department of Pharmacology and Toxicology, Medical University School, Lublin, Poland.
Kleinrok
Z
Z
Chodkowska
A
A
Szkodziak
A
A
Siembida
E
E
Gustaw
K
K
Urbanska
E
E
D000470:Alkaloids / Q000494:pharmacology*; D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001600:Berberine Alkaloids; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D010617:Phenanthridines; D019323:omega-N-Methylarginine / Q000494:pharmacology
D000470:Alkaloids; D018712:Analgesics, Non-Narcotic; D000970:Antineoplastic Agents; D001600:Berberine Alkaloids; D010617:Phenanthridines; D019323:omega-N-Methylarginine; D009569:Nitric Oxide; C060921:ukrain; D019331:NG-Nitroarginine Methyl Ester