title: Enhanced activity of carotid body chemoreceptors in rabbits with heart failure: role of nitric oxide.
abstract: An enhanced peripheral chemoreflex has been documented in patients with chronic heart failure (CHF). This study aimed to examine the characteristics of carotid body (CB) chemoreceptors in response to isocapnic hypoxia in a rabbit model of pacing-induced CHF and to evaluate the possible role that nitric oxide (NO) plays in the altered characteristics. The chemosensitive characteristics of the CB were evaluated by recording single-unit activity from the carotid sinus nerve in both an intact and a vascularly isolated preparation. It was found that the baseline discharge under normoxia (intact preparation: arterial PO2 90-95 Torr; isolated preparation: PO2 100-110 Torr) and the chemosensitivity in response to graded hypoxia (PO2 40-70 Torr) were enhanced in CHF vs. sham rabbits. These alterations were independent of the CB preparations (intact vs. isolated). NO synthase inhibition by Nomega-nitro-L-arginine increased the baseline discharge and the chemosensitivity in the intact preparation, whereas L-arginine (10(-5) M) inhibited the baseline discharge and the chemosensitivity in the isolated preparation in sham but not in CHF rabbits. S-nitroso-N-acetylpenicillamine, an NO donor, inhibited the baseline discharge and the chemosensitivity in both CB preparations in CHF rabbits but only in the isolated preparation in sham rabbits. The amount of NO produced in vitro by the CB under normoxia was less in CHF rabbits than in sham rabbits (P < 0.05). NO synthase-positive varicosities of nerve fibers within the CB were less in CHF rabbits than in sham rabbits (P < 0.05). These data indicate that an enhanced input from CB occurs in the rabbit model of pacing-induced CHF and that an impairment of NO production may contribute to this alteration.
authors: Department of Physiology and Biophysics, University of Nebraska College of Medicine, Omaha, Nebraska 68198-4575, USA.
authors: S Y
authors: SY
authors: Sun
authors: W
authors: W
authors: Wang
authors: I H
authors: IH
authors: Zucker
authors: H D
authors: HD
authors: Schultz
mesh_terms: D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002245:Carbon Dioxide / Q000097:blood; D002304:Cardiac Pacing, Artificial; D002344:Carotid Body / Q000503:physiopathology*; D002628:Chemoreceptor Cells / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D006333:Heart Failure / Q000503:physiopathology*; D000860:Hypoxia / Q000503:physiopathology; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D019335:Nitroarginine / Q000494:pharmacology; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D011817:Rabbits
chemical_list: D002245:Carbon Dioxide; D019335:Nitroarginine; D001120:Arginine; D019001:Nitric Oxide Synthase; D010100:Oxygen