Activation of nuclear transcription factor kappaB in rat carrageenin-induced pleurisy.
In this study we investigated the activation of nuclear factor-kappaB in the carrageenin-induced rat pleurisy. We found that nuclear factor-kappaB DNA binding activity, measured in inflammatory cells which migrated into the pleural cavity, was detectable at 3 and 6 h, markedly increased at 24 h and decreased at 48 h after induction of the inflammation. The increase in nuclear factor-kappaB DNA binding activity paralleled both exudate formation and leukocyte infiltration. Treatment of animals with pyrrolidine dithiocarbamate, an inhibitor of nuclear factor-kappaB activation, inhibited the nuclear factor-kappaB DNA binding activity as well as exudate formation and leukocyte infiltration. These results indicate that nuclear factor-kappaB is activated in the carrageenin-induced pleurisy and suggest that its inhibition may represent a novel strategy for the modulation of inflammatory response.
D'Acquisto
F
F
Department of Experimental Pharmacology, University of Naples Federico II, Italy.
Ianaro
A
A
Ialenti
A
A
Iuvone
T
T
Colantuoni
V
V
Carnuccio
R
R
D000818:Animals; D002351:Carrageenan / Q000633:toxicity*; D002465:Cell Movement; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005122:Exudates and Transudates / Q000378:metabolism; D007962:Leukocytes / Q000502:physiology; D008297:Male; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010998:Pleurisy / Q000139:chemically induced*; D011759:Pyrrolidines / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013859:Thiocarbamates / Q000494:pharmacology*; D013997:Time Factors
D004268:DNA-Binding Proteins; D016328:NF-kappa B; D011759:Pyrrolidines; D013859:Thiocarbamates; C020972:pyrrolidine dithiocarbamic acid; D002351:Carrageenan