Constitutive expression of interleukin-1beta (IL-1beta) in rat oligodendrocytes.
The RT-PCR analysis of RNA from progenitor and differentiated primary rat oligodendrocytes, and from the oligodendrocyte CG-4 cell line, shows the presence of the IL-1beta mRNA, the type I IL-1beta receptor and the IL-1 receptor accessory protein in these cells. In situ hybridization of a rat IL-1beta probe to primary progenitor and differentiated rat oligodendrocytes results in a positive signal. The double hybridization of the IL-1beta probe, together with an oligodendrocyte-specific differentiation marker, to sections of postnatal rat brain at different stages of differentiation is also positive. The double immuno-labelling technique utilized indicates coincidence of the signals on the brain slices. The results show that IL-1beta mRNA is constitutively expressed in rat brain oligodendrocytes from 1 day after birth onward. In agreement with this observation, CG-4 cells, primary progenitor and differentiated rat oligodendrocytes are positively stained by antibodies against IL-1beta. Postnatal brain slices from 1 and 4 day old and adult rats, labelled with a double immunofluorescence technique, are also stained by antibodies against IL-1beta. This signal coincides with that of antibodies against oligodendrocyte-specific surface markers. We conclude that IL-1beta is constitutively expressed in rat brain progenitor and differentiated oligodendrocytes.
Blasi
F
F
Dipartimento Biologia Evoluzionistica Sperimentale, Università di Bologna, Italy.
Riccio
M
M
Brogi
A
A
Strazza
M
M
Taddei
M L
ML
Romagnoli
S
S
Luddi
A
A
D'Angelo
R
R
Santi
S
S
Costantino-Ceccarini
E
E
Melli
M
M
D000818:Animals; D015870:Gene Expression; D007375:Interleukin-1 / Q000096:biosynthesis* / Q000235:genetics; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats
D007375:Interleukin-1