title: Prolonged release and c-kit expression of haemopoietic precursor cells mobilized by stem cell factor and granulocyte colony stimulating factor.
abstract: Mobilization of haemopoietic precursor cells into the circulation by the combination of cytokines, stem cell factor (SCF) and G-CSF in previously untreated patients with carcinoma of the breast resulted in increased yield of collected peripheral blood precursor cells (PBPC). This mobilization of PBPC by SCF with G-CSF lasted several days after ceasing the cytokines in comparison to the rapid fall of PBPC after ceasing G-CSF. Possible mechanisms for this increased and prolonged mobilization were investigated. Immunological phenotyping with CD38, Thy-1 and MDR-1 of the CD34-positive mobilized PBPC detected no difference in maturity compared to PBPC mobilized by G-CSF alone. However, the down-regulation of c-kit, which is associated with the mechanism of mobilization, was much greater in the PBPC mobilized by SCF and G-CSF. The potential clinical implication of increased and prolonged mobilization is increased yield, allowing transplantation of heavily pre-treated patients, transplantation with PBPC from a single apheresis, or PBSC support for multiple courses of high-dose therapy from one mobilization procedure.
authors: Haematology Department, Institute of Medical and Veterinary Science, Adelaide, South Australia, Australia.
authors: M M
authors: MM
authors: Roberts
authors: B W
authors: BW
authors: Swart
authors: P J
authors: PJ
authors: Simmons
authors: R L
authors: RL
authors: Basser
authors: C G
authors: CG
authors: Begley
authors: L B
authors: LB
authors: To
mesh_terms: D018952:Antigens, CD34; D001943:Breast Neoplasms / Q000378:metabolism / Q000628:therapy; D015331:Cohort Studies; D004338:Drug Combinations; D005260:Female; D005434:Flow Cytometry; D016179:Granulocyte Colony-Stimulating Factor / Q000627:therapeutic use*; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000378:metabolism*; D006801:Humans; D016130:Immunophenotyping; D007937:Leukapheresis / Q000379:methods; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D019089:Stem Cell Factor / Q000627:therapeutic use*
chemical_list: D018952:Antigens, CD34; D004338:Drug Combinations; D019089:Stem Cell Factor; D016179:Granulocyte Colony-Stimulating Factor; D019009:Proto-Oncogene Proteins c-kit