title: The human homolog of Drosophila cornichon protein is differentially expressed in alloactivated T-cells.
abstract: To identify novel genes induced in the early stage of T-cell activation, mRNA expression in alloactivated human lymphocytes was examined. Differential display-reverse transcription PCR analysis revealed a 207-bp cDNA fragment which was upregulated 24 h after allostimulation of a human T-cell line. The corresponding complete 1396 bp cDNA, named TGAM77, encodes a predicted 134 amino acid protein which shares 63% homology with the cornichon (cni) protein of Drosophila melanogaster. Upregulation of TGAM77 mRNA in the early phase of T-cell activation was confirmed by Northern blot and RT-PCR analysis of activated human lymphocytes. TGAM77 mRNA is expressed in a variety of human tissues with various expression levels. In analogy to cni which is involved in an epidermal growth factor-like signaling pathway inducing cellular asymmetry in Drosophila oogenesis, TGAM77 might function in similar signaling establishing vectorial re-localization and concentration of signaling events in T-cell activation.
authors: Institut für Medizinische Immunologie, Campus Mitte, Charité, Humboldt Universität, Schumannstrasse 20/21, 10098, Berlin, Germany. nalan.utku@charite.de
authors: N
authors: N
authors: Utku
authors: G C
authors: GC
authors: Bulwin
authors: S
authors: S
authors: Beinke
authors: T
authors: T
authors: Heinemann
authors: F
authors: F
authors: Beato
authors: J
authors: J
authors: Randall
authors: B
authors: B
authors: Schnieders
authors: K
authors: K
authors: Sandhoff
authors: H D
authors: HD
authors: Volk
authors: E
authors: E
authors: Milford
authors: S R
authors: SR
authors: Gullans
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000737:chemistry*; D029721:Drosophila Proteins; D004331:Drosophila melanogaster / Q000235:genetics; D004527:Egg Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008213:Lymphocyte Activation / Q000235:genetics; D008565:Membrane Proteins; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000032:analysis; D012689:Sequence Homology, Nucleic Acid; D015398:Signal Transduction / Q000235:genetics; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: C118276:CNIH1 protein, human; C485558:Cnih protein, mouse; D018076:DNA, Complementary; D029721:Drosophila Proteins; D004527:Egg Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C485557:cni protein, Drosophila