title: A 1-Mb BAC/PAC-based physical map of the autosomal recessive polycystic kidney disease gene (PKHD1) region on chromosome 6.
abstract: The PKHD1 (polycystic kidney and hepatic disease 1) gene responsible for autosomal recessive polycystic kidney disease has been mapped to 6p21.1-p12 to an approximately 1-cM interval flanked by the markers D6S1714/D6S243 and D6S1024. We have developed a sequence-ready BAC/PAC-based contig map of this region as the next step for the positional cloning of PKHD1. This contig comprising 52 clones spanning approximately 1 Mb was established by content mapping of 44 BAC/PAC-end-derived STSs, 3 known genetic markers, 5 YAC-end-derived STSs, 3 random STSs, 1 previously mapped gene, and 1 EST. The average depth per marker is 6.3 clones, and the average STS density is 20 kb. The genomic clone overlaps were confirmed by restriction fragment fingerprint analysis. A high-resolution BAC/PAC-based contig map is essential to the ultimate goal of identifying the PKHD1 gene.
authors: Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
authors: J H
authors: JH
authors: Park
authors: M P
authors: MP
authors: Dixit
authors: L F
authors: LF
authors: Onuchic
authors: G
authors: G
authors: Wu
authors: A N
authors: AN
authors: Goncharuk
authors: S
authors: S
authors: Kneitz
authors: L B
authors: LB
authors: Santarina
authors: T
authors: T
authors: Hayashi
authors: E D
authors: ED
authors: Avner
authors: L
authors: L
authors: Guay-Woodford
authors: K
authors: K
authors: Zerres
authors: G G
authors: GG
authors: Germino
authors: S
authors: S
authors: Somlo
mesh_terms: D002896:Chromosomes, Human, Pair 6 / Q000235:genetics*; D020451:Contig Mapping; D004247:DNA / Q000032:analysis / Q000235:genetics*; D016172:DNA Fingerprinting; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D017044:Polycystic Kidney, Autosomal Recessive / Q000235:genetics*; D016324:Sequence Tagged Sites
chemical_list: D004247:DNA