title: F-Spondin, expressed in somite regions avoided by neural crest cells, mediates inhibition of distinct somite domains to neural crest migration.
abstract: Neural crest (NC) cells migrate exclusively into the rostral half of each sclerotome, where they avoid the dermomyotome and the paranotochordal sclerotome. F-spondin is expressed in these inhibitory regions and throughout the caudal halves. In vitro bioassays of NC spreading on substrates of rostral or caudal epithelial-half somites (RS or CS, respectively) revealed that NC cells adopt on RS a fibroblastic morphology, whereas on CS they fail to flatten. F-spondin inhibited flattening of NC cells on RS. Conversely, F-spondin antibodies prevented rounding up of NC cells on CS. Addition of F-spondin to trunk explants inhibited NC migration into the sclerotome, and treatment of embryos with anti-F-spondin antibodies yielded migration into otherwise inhibitory sites. Thus, somite-derived F-spondin is an inhibitory signal involved in patterning the segmental migration of NC cells and their topographical segregation within the RS.
authors: Department of Anatomy and Cell Biology, Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel.
authors: A
authors: A
authors: Debby-Brafman
authors: T
authors: T
authors: Burstyn-Cohen
authors: A
authors: A
authors: Klar
authors: C
authors: C
authors: Kalcheim
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D006133:Growth Substances; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008845:Microinjections; D018613:Microscopy, Confocal; D019006:Neural Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D009432:Neural Crest / Q000166:cytology* / Q000196:embryology; D009474:Neurons / Q000502:physiology* / Q000648:ultrastructure; D010455:Peptides; D012333:RNA, Messenger / Q000096:biosynthesis; D015398:Signal Transduction / Q000502:physiology; D019170:Somites / Q000378:metabolism*
chemical_list: D006133:Growth Substances; D019006:Neural Cell Adhesion Molecules; D010455:Peptides; D012333:RNA, Messenger