title: Performance of sampling plans to determine aflatoxin in farmers' stock peanut lots by measuring aflatoxin in high-risk-grade components.
abstract: Five 2 kg test samples were taken from each of 120 farmers' stock peanut lots contaminated with aflatoxin. Kernels from each 2 kg sample were divided into the following U.S. Department of Agriculture grade components: sound mature kernels plus sound splits (SMKSS), other kernels (OK), loose shelled kernels (LSK), and damaged kernels (DAM). The kernel mass (g), aflatoxin mass (ng), and aflatoxin concentration (ng of aflatoxin/g of peanuts) were measured for each of the 2400 component samples. The variabilities associated with measuring aflatoxin mass (ng) in OK + LSK + DAM, or A(OLD)ng, and in LSK + DAM, or A(LD)ng, and aflatoxin concentration (ng/g) in OK + LSK + DAM, or A(OLD)ng/g, and in LSK + DAM, or A(LD)ng/g, were determined. The variance associated with measuring aflatoxin in each of the 4 combinations of components increased with aflatoxin, and functional relationships were developed from regression analysis. The variability associated with estimating the lot concentration from each of the 4 combinations of components was also determined. The coefficients of variation (CV) associated with estimating the aflatoxin for a lot with aflatoxin at 100 ng/g were 90, 86, 94 and 96% for aflatoxin masses A(OLD)ng and A(LD)ng and aflatoxin concentrations A(OLD)ng/g and A(LD)ng/g, respectively. The performance of aflatoxin sampling plans using the combination of aflatoxin masses in OK + LD + DAM and LD + DAM components was evaluated with a 2 kg test sample and a 50 ng/g accept/reject limit.
authors: U.S. Department of Agriculture, North Carolina State University, Agricultural Research Service, Raleigh, NC 27695-7625, USA.
authors: T B
authors: TB
authors: Whitaker
authors: W M
authors: WM
authors: Hagler
authors: F G
authors: FG
authors: Giesbrecht
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D000383:Agriculture; D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination; D011786:Quality Control; D012044:Regression Analysis
chemical_list: D000348:Aflatoxins