title: Isolation and characterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase.
abstract: A cDNA clone encoding 10-formyltetrahydrofolate dehydrogenase (10-FTHFDH) was isolated from a human fetal liver cDNA library. It contained the open reading frame of 2,709 base pairs and predicted a protein comprising 902 amino acids with a calculated molecular weight of 98,700 Da. The deduced protein showed about 93.6% homology (90.5% identity, 3.1% favored substitutions) when compared with rat 10-FTHFDH. The distribution of 10-FTHFDH transcript in various human tissues was studied by Northern blot analysis using poly(A+) RNAs from different tissues. The 10-FTHFDH transcript with an approximate size of 2.7 kb was mainly expressed in human kidney, skeletal muscle, and liver and rarely expressed in other tissues.
authors: Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
authors: M
authors: M
authors: Hong
authors: Y
authors: Y
authors: Lee
authors: J W
authors: JW
authors: Kim
authors: J S
authors: JS
authors: Lim
authors: S Y
authors: SY
authors: Chang
authors: K S
authors: KS
authors: Lee
authors: S G
authors: SG
authors: Paik
authors: I S
authors: IS
authors: Choe
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008099:Liver / Q000196:embryology / Q000201:enzymology*; D008969:Molecular Sequence Data; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry / Q000235:genetics*; D012333:RNA, Messenger / Q000378:metabolism; D015183:Restriction Mapping; D017386:Sequence Homology, Amino Acid; D000099295:Formyltetrahydrofolate Dehydrogenase
chemical_list: D018076:DNA, Complementary; D000587:Oxidoreductases Acting on CH-NH Group Donors; D012333:RNA, Messenger; D000099295:Formyltetrahydrofolate Dehydrogenase