Testis-specific TTF-D binds to single-stranded DNA in the c-mos and Odf1 promoters and activates Odf1.
We recently identified testis-specific nuclear factor binding sites in the testis-specific promoters of the c-mos gene and the Odf1 gene, which are 80% identical. Here we characterize a testis-specific nuclear factor, TTF-D, which is able to complex with both binding sites and stimulates Odf1 promoter activity. TTF-D is detectable in mouse testis as early as day 11 postpartum and contains three peptides of 22, 25, and 35 kDa in size. Surprisingly, TTF-D binds specifically to its cognate double-stranded DNA binding site as well as to its single-stranded DNA binding site. Both double-stranded and single-stranded binding site oligonucleotide DNA can specifically repress Odf1 promoter activity. Our results suggest that TTF-D is involved in positive transcription regulation of a pre-meiotic and a post-meiotic gene in the testis.
Oosterhuis
J H
JH
Department of Biochemistry & Molecular Biology, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N 4N1, Canada.
van der Hoorn
F A
FA
D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006360:Heat-Shock Proteins; D008297:Male; D051379:Mice; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D011506:Proteins / Q000235:genetics*; D017231:Proto-Oncogene Proteins c-mos / Q000235:genetics*; D051381:Rats; D013737:Testis / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D017931:DNA Primers; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D009687:Nuclear Proteins; C490109:Odf1 protein, mouse; C490108:Odf1 protein, rat; D011506:Proteins; D014157:Transcription Factors; D017231:Proto-Oncogene Proteins c-mos