Assembly requirements and role of CotH during spore coat formation in Bacillus subtilis.
We report Western blot data showing that the 42.8-kDa product of the previously characterized cotH locus (8) is a structural component of the Bacillus subtilis spore coat. We show that the assembly of CotH requires both CotE and GerE. In agreement with these observations, the ultrastructural analysis of purified spores suggests that CotH is needed for proper formation of both inner and outer layers of the coat.
Zilhão
R
R
Department of Plant Physiology, University of Lisbon, Campo Grande Bl C2, 1700 Lisbon, Portugal.
Naclerio
G
G
Henriques
A O
AO
Baccigalupi
L
L
Moran
C P
CP
Ricca
E
E
D001412:Bacillus subtilis / Q000502:physiology* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism*; D002473:Cell Wall / Q000737:chemistry; D012808:Sigma Factor; D013171:Spores, Bacterial / Q000502:physiology / Q000648:ultrastructure; D014157:Transcription Factors
D001426:Bacterial Proteins; C077494:CotE protein, Bacillus subtilis; C101016:CotH protein, Bacillus subtilis; D012808:Sigma Factor; D014157:Transcription Factors; C092468:spoIIR protein, Bacillus subtilis; C030069:spore-specific proteins, Bacillus