title: Downregulation of Galphaq-11 protein expression in guinea pig antral and colonic circular muscle during pregnancy.
abstract: Pregnancy has an inhibitory effect on motility of the gastrointestinal tract. The present study was designed to examine the mechanisms responsible for antral and colonic hypomotility in pregnant guinea pigs. Circular smooth muscle cells from the antrum and left colon were isolated by enzymatic digestion with collagenase from pregnant and nonpregnant guinea pigs. Contractile responses to agonists were expressed as percent shortening from resting cell length. The function of G proteins in antral and colonic circular smooth muscle was assessed by [35S]guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) binding induced by CCK-8 and G protein quantitation. The contraction of antral and colonic circular smooth muscle from pregnant guinea pigs was reduced in response to CCK-8 and to GTPgammaS but was normal in response to KCl and D-myo-inositol 1,4,5-trisphosphate compared with nonpregnant animals. The stimulation of [35S]GTPgammaS binding to Galphaq-11 induced by 1 microM CCK-8 was significantly lower in antral and colonic circular smooth muscle from pregnant guinea pigs than that in controls. Furthermore, Western blot analysis showed a decreased Galphaq-11 and an increased Gsalpha protein content in both tissues during pregnancy. It is concluded that pregnancy appears to impair gastrointestinal circular smooth muscle contractility by downregulating G proteins such as Galphaq-11 protein, which mediates muscle contraction, and upregulating Gsalpha protein, which mediates muscle relaxation.
authors: Department of Medicine, Rhode Island Hospital and Brown University School of Medicine, Providence, Rhode Island 02903, USA.
authors: Q
authors: Q
authors: Chen
authors: Z L
authors: ZL
authors: Xiao
authors: P
authors: P
authors: Biancani
authors: J
authors: J
authors: Behar
mesh_terms: D000818:Animals; D003106:Colon / Q000187:drug effects / Q000502:physiology*; D005260:Female; D019204:GTP-Binding Proteins / Q000235:genetics*; D005769:Gastrointestinal Motility / Q000187:drug effects / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology*; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D015544:Inositol 1,4,5-Trisphosphate / Q000494:pharmacology; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology*; D011247:Pregnancy; D011270:Pregnancy, Animal / Q000502:physiology*; D011706:Pyloric Antrum; D012844:Sincalide / Q000494:pharmacology; D013270:Stomach / Q000187:drug effects / Q000502:physiology*
chemical_list: D016244:Guanosine 5'-O-(3-Thiotriphosphate); D015544:Inositol 1,4,5-Trisphosphate; D019204:GTP-Binding Proteins; D012844:Sincalide