title: Induction of epithelial Na+ channel in rat ileum after proctocolectomy.
abstract: In patients with colectomy, epithelial transport function in the remnant small intestine can be regulated in response to the increased fecal electrolyte and fluid loss. Using a rat colectomy model, we investigated the Na+ and K+ transport mechanisms underlying the intestinal response. Proctocolectomy with ileoanal anastomosis was performed on rats. The small intestinal mucosa was mounted in Ussing chambers; then short-circuit currents and 22Na+ fluxes were measured. mRNA expression of the epithelial Na+ channel (ENaC) was determined by Northern blotting. Amiloride-sensitive, electrogenic Na+ absorption appeared in the ileum after proctocolectomy. This functional change was accompanied by the chronological induction of mRNAs for alpha-, beta-, and gamma-subunits of the ENaC in the ileum. Tetraethylammonium-sensitive short-circuit current was also activated. We conclude that electrogenic Na+ absorption and probably K+ secretion are induced in the ileum after proctocolectomy. This induction of electrogenic Na+ absorption is probably mediated by the increase in the mRNA levels for all three types of subunits of the ENaC and may contribute to the recovery from the increased fecal Na+ loss.
authors: First Department of Surgery, Tohoku University School of Medicine, Sendai 980-0872, Japan.
authors: K
authors: K
authors: Koyama
authors: I
authors: I
authors: Sasaki
authors: H
authors: H
authors: Naito
authors: Y
authors: Y
authors: Funayama
authors: K
authors: K
authors: Fukushima
authors: M
authors: M
authors: Unno
authors: S
authors: S
authors: Matsuno
authors: H
authors: H
authors: Hayashi
authors: Y
authors: Y
authors: Suzuki
mesh_terms: D000450:Aldosterone / Q000097:blood / Q000652:urine; D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001834:Body Water / Q000378:metabolism; D001835:Body Weight; D003345:Corticosterone / Q000097:blood / Q000652:urine; D053503:Epithelial Sodium Channels; D005243:Feces; D007082:Ileum; D007413:Intestinal Mucosa / Q000187:drug effects / Q000502:physiology*; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D016737:Proctocolectomy, Restorative; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012964:Sodium / Q000378:metabolism*; D015222:Sodium Channels / Q000235:genetics*; D019789:Tetraethylammonium / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D053503:Epithelial Sodium Channels; D012333:RNA, Messenger; D015222:Sodium Channels; D000450:Aldosterone; D019789:Tetraethylammonium; D000584:Amiloride; D012964:Sodium; D011188:Potassium; D003345:Corticosterone