Application of overpressured layer chromatography combined with digital autoradiography and mass spectrometry in the study of deramciclane metabolism.
Overpressured layer chromatography was combined with the highly sensitive and rapid digital autoradiography (DAR) and mass spectrometry to separate, detect, and identify 3H- and 14C-labeled deramciclane metabolites in different biological matrixes. Several minor and major metabolites were separated from plasma and urine samples. The radioactive metabolites localized by DAR were scraped from the thin-layer chromatographic plate and transferred to a mass spectrometer for structure identification. Several metabolites were isolated and characterized, including hydroxy-N-desmethyl deramciclane, which is described in detail. The combination of techniques is efficient and has good sensitivity: about 2 micrograms metabolite from a biological matrix was isolated and identified this way.
Ludányi
K
K
Institute of Chemistry of the Hungarian Academy of Sciences, Budapest, Hungary.
Vékey
K
K
Szúnyog
J
J
Mincsovics
E
E
Karancsi
T
T
Ujszászy
K
K
Nemes
K B
KB
Klebovich
I
I
D000818:Animals; D014151:Anti-Anxiety Agents / Q000493:pharmacokinetics*; D001345:Autoradiography / Q000379:methods*; D001892:Camphanes / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D002250:Carbon Radioisotopes; D002845:Chromatography / Q000379:methods*; D004285:Dogs; D013058:Mass Spectrometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012702:Serotonin Antagonists / Q000493:pharmacokinetics*; D014316:Tritium
D014151:Anti-Anxiety Agents; D001892:Camphanes; D002250:Carbon Radioisotopes; D012702:Serotonin Antagonists; D014316:Tritium; C060555:deramciclane