title: Characterization of katD, a kinesin-like protein gene specifically expressed in floral tissues of Arabidopsis thaliana.
abstract: Kinesin and kinesin-like proteins (KLPs) are microtubule-based motor proteins that play important roles in organelle transport. Based on the homology to these proteins, a katD cDNA has now been isolated from a library prepared from flowers of Arabidopsis thaliana ecotype Columbia. Sequence analysis of the katD cDNA revealed an open reading frame of 2691bp [corrected], encoding a protein of 987 amino acids. Comparison of the nucleotide sequences of katD genomic and cDNA clones revealed the presence of 18 introns, 17 of which conform to the GU-AG rule. The central region of the KatD polypeptide exhibits substantial amino acid sequence homology to the motor domain of kinesin heavy chains, although the motor domain of KatD appears to be phylogenetically distant from those of other KLPs in plants. The amino-terminal region of KatD shares marked sequence similarity with the calponin homology domain, whereas the approximately 240-residue carboxyl-terminal region shows no significant homology to other known proteins. The predicted secondary structure of KatD revealed the lack of an alpha-helical coiled coil structure typical of kinesin heavy chains, suggesting that KatD may function as a monomeric motor. A recombinant truncated KatD protein containing the putative motor domain was shown both to bind to mammalian microtubules in a manner dependent on a non-hydrolyzable ATP analog, and to possess microtubule-dependent ATPase activity. Immunoblot and Northern blot analyses showed that both KatD protein and mRNA are expressed specifically in floral tissues. These results suggest that the structurally distinct KatD protein functions as a floral tissue-specific motor protein.
authors: Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
authors: K
authors: K
authors: Tamura
authors: K
authors: K
authors: Nakatani
authors: H
authors: H
authors: Mitsui
authors: Y
authors: Y
authors: Ohashi
authors: H
authors: H
authors: Takahashi
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins; D001483:Base Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry; D003001:Cloning, Molecular; D017343:Genes, Plant; D016547:Kinesins / Q000235:genetics; D008840:Microfilament Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D016415:Sequence Alignment; D000096985:Calponins
chemical_list: D000251:Adenosine Triphosphatases; D029681:Arabidopsis Proteins; D002135:Calcium-Binding Proteins; D016547:Kinesins; D008840:Microfilament Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D000096985:Calponins; C118911:KatD protein, Arabidopsis