Multiresidue screening of pesticides in foods using retention time locking, GC-AED, database search, and GC/MS identification.
Fruit and vegetable extracts were screened for over 400 pesticides by gas chromatography with atomic emission detection (GC-AED) and an experimental database. A technique called retention time locking was used to match GC-AED and GC with mass spectrometry (MS) retention times to those of the database. Samples were analyzed for sulfur, nitrogen, phosphorus, and chlorine by GC-AED. Possible pesticides were suggested by database search and identified by GC/MS. Forty-four pesticide standards were analyzed to determine the precision of retention time matching and the accuracy of the database search. Analytical retention times matched database retention times within 0.32 min. Using elemental criteria, the database search identified the correct compound for 41 of 44 pesticide standards. For blind spikes of fruit and vegetable extracts, the database suggested 22 of 26 spiked pesticides as matches. Nineteen were identified by GC/MS. The combination of retention time locking, GC-AED, database search, and GC/MS can be a powerful tool for identifying pesticides in a complex matrix.
Cook
J
J
Florida Department of Agriculture and Consumer Services, Chemical Residue Laboratory, Tallahassee 32219-1650, USA.
Engel
M
M
Wylie
P
P
Quimby
B
B
D002713:Chlorine / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D016208:Databases, Factual; D005504:Food Analysis / Q000379:methods*; D005638:Fruit / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D016247:Information Storage and Retrieval; D009584:Nitrogen / Q000032:analysis; D010573:Pesticide Residues / Q000032:analysis*; D010758:Phosphorus / Q000032:analysis; D012015:Reference Standards; D012680:Sensitivity and Specificity; D013455:Sulfur / Q000032:analysis; D014675:Vegetables / Q000737:chemistry
D010573:Pesticide Residues; D010758:Phosphorus; D002713:Chlorine; D013455:Sulfur; D009584:Nitrogen