title: Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature.
abstract: We localized five rpoC (beta') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed beta' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved beta' residues participate in interactions holding RNA polymerase together.
authors: Waksman Institute and Department of Genetics, Rutgers, The State University, Piscataway, New Jersey 08854, USA.
authors: E C
authors: EC
authors: Nedea
authors: D
authors: D
authors: Markov
authors: T
authors: T
authors: Naryshkina
authors: K
authors: K
authors: Severinov
mesh_terms: D000595:Amino Acid Sequence; D017124:Conserved Sequence; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D008969:Molecular Sequence Data; D017354:Point Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
chemical_list: C108570:beta' subunit of RNA polymerase; D012321:DNA-Directed RNA Polymerases