Cyclic AMP can decrease expression of genes subject to catabolite repression in Saccharomyces cerevisiae.
External cyclic AMP (cAMP) hindered the derepression of gluconeogenic enzymes in a pde2 mutant of Saccharomyces cerevisiae, but it did not prevent invertase derepression. cAMP reduced nearly 20-fold the transcription driven by upstream activation sequence (UAS1FBP1) from FBP1, encoding fructose-1,6-bisphosphatase; it decreased 2-fold the activation of transcription by UAS2FBP1. Nuclear extracts from cells derepressed in the presence of cAMP were impaired in the formation of specific UASFBP1-protein complexes in band shift experiments. cAMP does not appear to act through the repressing protein Mig1. Control of FBP1 transcription through cAMP is redundant with other regulatory mechanisms.
Zaragoza
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O
Instituto de Investigaciones Biomédicas "Alberto Sols," CSIC-UAM, 28029 Madrid, Spain.
Lindley
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C
Gancedo
J M
JM
D002262:Carboxy-Lyases / Q000096:biosynthesis / Q000235:genetics; D000242:Cyclic AMP / Q000494:pharmacology*; D004794:Enzyme Repression; D005092:Exonucleases / Q000172:deficiency; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D005943:Gluconeogenesis / Q000187:drug effects; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D011485:Protein Binding; D012045:Regulatory Sequences, Nucleic Acid; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology; D014157:Transcription Factors / Q000378:metabolism
D014157:Transcription Factors; D000242:Cyclic AMP; D005969:Glutamate Dehydrogenase; D005092:Exonucleases; C022650:spleen exonuclease; D006597:Fructose-Bisphosphatase; D002262:Carboxy-Lyases