Anticancer drugs inhibit induction of NO synthase in rat in vivo.
Nitric oxide (NO), synthesized by inducible NO synthase (iNOS) in immunoreactive cells, plays important roles in their activities such as bactericidal and tumoricidal functions. We examined the distribution of iNOS and evaluated the effects of anticancer drugs, 4'-epi-doxorubicin (EPI-DXR) and mitomycin C (MMC), on iNOS induction by lipopolysaccharide in rats. Ascites cells and bone marrow showed the highest induction of iNOS in the tested organs. Administration of EPI-DXR to rats strongly inhibited iNOS induction in lung, ascites, and bone marrow, but only slightly in liver and spleen. MMC administration inhibited the induction in the most immune reactive organs.
Inagaki
R
R
Department of Radiology, School of Medicine, Fukui Medical University, Japan.
Taniguchi
T
T
Sakai
T
T
Hayashi
N
N
Ishii
Y
Y
Muramatsu
I
I
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D015251:Epirubicin / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D016685:Mitomycin / Q000494:pharmacology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II; D051381:Rats; D017208:Rats, Wistar; D014018:Tissue Distribution / Q000187:drug effects
D000903:Antibiotics, Antineoplastic; D008070:Lipopolysaccharides; D015251:Epirubicin; D016685:Mitomycin; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II; C496318:Nos2 protein, rat