Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8).
We report the cloning of a cDNA encoding human syntaxin 8 (STX8), using the regulator (R) domain of the cystic fibrosis transmembrane conductance regulator (CFTR) as a bait to screen a human fetal lung cDNA library by the yeast two-hybrid system. This gene was found broadly transcribed and its mRNA size is about 1.3 kb. The STX8 gene maps to chromosomal band 17p12 and it encodes a 236-amino-acid protein. Syntaxin 8 contains in its C-terminal half a coiled-coil domain found highly conserved in the t-SNARE (SNAP receptor on target membrane) superfamily of proteins, which are involved in vesicular trafficking and docking. In syntaxin 8, a C-terminal hydrophobic domain may constitute a transmembrane anchor. It was recently shown that CFTR-mediated chloride currents can be regulated by syntaxin 1A, a t-SNARE family member, through direct protein-protein interaction. This raises the possibility that syntaxin 8 may also be involved in such regulations.
Thoreau
V
V
UPRES 2622, CHU de Poitiers, France.
Bergès
T
T
Callebaut
I
I
Guillier-Gencik
Z
Z
Gressin
L
L
Bernheim
A
A
Karst
F
F
Mornon
J P
JP
Kitzis
A
A
Chomel
J C
JC
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015152:Blotting, Northern; D002874:Chromosome Mapping; D002886:Chromosomes, Human, Pair 17 / Q000235:genetics*; D003001:Cloning, Molecular; D017124:Conserved Sequence; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000196:embryology / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011485:Protein Binding; D017433:Protein Structure, Secondary; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D050600:SNARE Proteins; D017386:Sequence Homology, Amino Acid; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
C505032:CFTR protein, human; D008565:Membrane Proteins; D050765:Qa-SNARE Proteins; D012333:RNA, Messenger; D050600:SNARE Proteins; C494212:STX1A protein, human; D050827:Syntaxin 1; D033921:Vesicular Transport Proteins; D019005:Cystic Fibrosis Transmembrane Conductance Regulator