title: How does arrestin respond to the phosphorylated state of rhodopsin?
abstract: Visual arrestin quenches light-induced signaling by binding to light-activated, phosphorylated rhodopsin (P-Rh*). Here we present structure-function data, which in conjunction with the refined crystal structure of arrestin (Hirsch, J. A., Schubert, C., Gurevich, V. V., and Sigler, P. B. (1999) Cell, in press), support a model for the conversion of a basal or "inactive" conformation of free arrestin to one that can bind to and inhibit the light activated receptor. The trigger for this transition is an interaction of the phosphorylated COOH-terminal segment of the receptor with arrestin that disrupts intramolecular interactions, including a hydrogen-bonded network of buried, charged side chains, referred to as the "polar core." This disruption permits structural adjustments that allow arrestin to bind to the receptor. Our mutational survey identifies residues in arrestin (Arg175, Asp30, Asp296, Asp303, Arg382), which when altered bypass the need for the interaction with the receptor's phosphopeptide, enabling arrestin to bind to activated, nonphosphorylated rhodopsin (Rh*). These mutational changes disrupt interactions and substructures which the crystallographic model and previous biochemical studies have shown are responsible for maintaining the inactive state. The molecular basis for these disruptions was confirmed by successfully introducing structure-based second site substitutions that restored the critical interactions. The nearly absolute conservation of the mutagenically sensitive residues throughout the arrestin family suggests that this mechanism is likely to be applicable to arrestin-mediated desensitization of most G-protein-coupled receptors.
authors: Ralph and Muriel Roberts Laboratory for Vision Science, Sun Health Research Institute, Sun City, Arizona 85372, USA.
authors: S A
authors: SA
authors: Vishnivetskiy
authors: C L
authors: CL
authors: Paz
authors: C
authors: C
authors: Schubert
authors: J A
authors: JA
authors: Hirsch
authors: P B
authors: PB
authors: Sigler
authors: V V
authors: VV
authors: Gurevich
mesh_terms: D000818:Animals; D019393:Arrestin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D016297:Mutagenesis, Site-Directed; D010766:Phosphorylation; D011487:Protein Conformation; D012243:Rhodopsin / Q000378:metabolism*
chemical_list: D019393:Arrestin; D012243:Rhodopsin