Characterization of toad liver glutathione transferase.
The major form of glutathione transferase from the toad liver previously designed as Bufo bufo liver GST-7.6 (A. Aceto, B. Dragani, T. Bucciarelli, P. Sacchetta, F. Martini, S. Angelucci, F. Amicarelli, M. Miranda and C. Di Ilio, Biochem. J. 289 (1993) 417-422) has been characterized. According to its partial amino acid sequence, the toad enzyme may be included in the pi class GST and named bbGST P2-2. However, bbGST P2-2 appears to be immunologically, structurally and kinetically distinct from any other members of pi family, including bbGST P1-1, suggesting that it may constitute a subset of pi class GST. The data support the hypothesis that the transition from aquatic to terrestrial life causes a switch of the GST amphibian pattern promoting the expression of a GST form (bbGST P2-2) able to counteract, with higher efficiency, the toxic effects of reactive metabolites of oxidative metabolism and those of hydrophobic xenobiotics.
Bucciarelli
T
T
Dipartimento di Scienze Biomediche, Università 'G. D'Annunzio', 66100, Chieti, Italy.
Sacchetta
P
P
Pennelli
A
A
Cornelio
L
L
Romagnoli
R
R
Melino
S
S
Petruzzelli
R
R
Di Ilio
C
C
D000595:Amino Acid Sequence; D000663:Amphibians; D000818:Animals; D002023:Bufo bufo; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008969:Molecular Sequence Data; D013439:Sulfhydryl Reagents; D013696:Temperature
D013439:Sulfhydryl Reagents; D005982:Glutathione Transferase