Detection of Chlamydia pneumoniae DNA in atheromatous tissues by polymerase chain reaction.
Chlamydia pneumoniae is an important human respiratory pathogen. Recently, seroepidemiological data and the demonstration of chlamydial DNA or antigen within parts of atherosclerotic lesions have suggested a causal association between chlamydial infections and atherosclerosis. As the results obtained by different groups show considerable variations, we provide further data based on a highly specific and sensitive nested PCR method. Positivity was confirmed by nonradioactive hybridization with a specific probe, and sensitivity was determined by titration experiments. C. pneumoniae DNA was detected in 8/29 (28%) of carotid artery samples and 3/14 (21%) of aortic aneurysms.
Nadrchal
R
R
Klinische Abteilung für Klinische Mikrobiologie des Klinischen Institutes für Hygiene, Vienna, Austria.
Makristathis
A
A
Apfalter
P
P
Rotter
M
M
Trubel
W
W
Huk
I
I
Polterauer
P
P
Hirschl
A M
AM
D000368:Aged; D000369:Aged, 80 and over; D001161:Arteriosclerosis / Q000473:pathology* / Q000601:surgery; D002339:Carotid Arteries / Q000473:pathology; D016893:Carotid Stenosis / Q000473:pathology* / Q000601:surgery; D002690:Chlamydia Infections / Q000473:pathology*; D016993:Chlamydophila pneumoniae; D004269:DNA, Bacterial / Q000032:analysis*; D016894:Endarterectomy, Carotid; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D004269:DNA, Bacterial