Screening of antioxidant action of various molds and protection of Monascus anka against experimentally induced liver injuries of rats.
Antioxidant action of various molds, which are traditionally used for the production of foods or alcoholic beverages in Japan, was studied in vitro and in vivo. Antioxidant action was evaluated by scavenging stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and lipid peroxidation of rat liver microsomes. Among 40 molds, 16 species showed the DPPH scavenging action, and the molds that can scavenge the DPPH radical inhibited lipid peroxidation. The mold with the strongest action, Monascus anka, was chosen for the investigation of a protective action against liver injury of rats. When galactosamine (GalN, 400 mg/kg) or GalN plus lipopolysaccharide (LPS, 0.5 microg/kg) was given intraperitoneally to rats (Sprague-Dawley), aspartate aminotransferase (AST) and glutathione (GSH) S-transferase (GST) activities in serum were significantly increased. However, such hepatotoxicities seen in the increase in serum enzyme levels were depressed when the extract prepared from M. anka was given 1 and 15 h before the toxic insultant. Liver microsomal GST activity, which is known to be activated by oxidative stress, was increased by GalN or GaIN plus LPS treatment and the increase was also inhibited by pretreatment with the extract. Pathomorphological changes in the liver caused by GalN treatment also were prevented by the mold extract. These results indicate that the extract of M. anka has radical scavenging action and ameliorates chemically induced hepatotoxicity.
Aniya
Y
Y
Laboratory of Physiology and Pharmacology, School of Health Sciences, University of the Ryukyus, Okinawa, Japan. yaniya@med.u-ryukyu.ac.jp
Yokomakura
T
T
Yonamine
M
M
Shimada
K
K
Nagamine
T
T
Shimabukuro
M
M
Gibo
H
H
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D001230:Aspergillus / Q000737:chemistry; D002457:Cell Extracts / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005688:Galactosamine / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley
D000975:Antioxidants; D002457:Cell Extracts; D005688:Galactosamine; D005982:Glutathione Transferase; D004229:Dithiothreitol