Transportation and storage of cryosections in PBS-Dulbecco.
Tissue pieces that cannot be fixed and embedded before immunostaining must be cryosectioned and stained quickly. We describe an easy and reliable technique to keep cryosections with immune complexes and complement stored for several weeks until the final immunostaining. The same technique was used for long distance transportation of cryosections and it was extensively tested for human kidney biopsies with excellent results.
Eneström
S
S
Division of Molecular and Immunological Pathology, Linköping University, Sweden. sveen@pai.liu.se
Nilsson
A
A
Peen
E
E
D000818:Animals; D002021:Buffers; D016616:Cryoultramicrotomy / Q000379:methods*; D006801:Humans; D010710:Phosphates; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
D002021:Buffers; D010710:Phosphates; C018279:sodium phosphate