Depression of liver protein synthesis during surgery is prevented by growth hormone.
This study was undertaken to elucidate the specific effects of growth hormone (GH) on liver protein metabolism in humans during surgery. Otherwise healthy patients scheduled for elective laparoscopic cholecystectomy were randomized into controls (n = 9) or pretreatment with 12 units of GH for 1 day (GH 1, n = 9) or daily for 5 days (GH 5, n = 10). The fractional synthesis rate of liver proteins, as assessed by flooding with [2H5]phenylalanine, was higher in the GH 5 group (22.0 +/- 6.9%/day, mean +/- SD, P < 0.05) than in the control (16.1 +/- 3.1%/day) and GH 1 (16.5 +/- 5.5%/day) groups. During surgery, the fraction of polyribosomes in the liver, as assessed by ribosome analysis, decreased in the control group by approximately 12% (P < 0.01) but did not decrease in the GH-treated groups. In addition, the concentrations of the essential amino acids and aspartate in the liver decreased in response to GH treatment. In conclusion, GH pretreatment decreases hepatic free amino acid concentrations and preserves liver protein synthesis during surgery.
Barle
H
H
Department of Anesthesiology and Intensive Care, Huddinge University Hospital, S-141 86 Huddinge, Sweden. barle@ebox.tninet.se
Essén
P
P
Nyberg
B
B
Olivecrona
H
H
Tally
M
M
McNurlan
M A
MA
Wernerman
J
J
Garlick
P J
PJ
D000328:Adult; D000596:Amino Acids / Q000097:blood / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D017081:Cholecystectomy, Laparoscopic; D004334:Drug Administration Schedule; D005260:Female; D019382:Human Growth Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D007279:Injections, Subcutaneous; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000648:ultrastructure; D014508:Urea / Q000097:blood
D000596:Amino Acids; D001786:Blood Glucose; D011506:Proteins; D011994:Recombinant Proteins; D019382:Human Growth Hormone; D014508:Urea