title: Immunological localization and ontogenetic development of inhibin alpha subunit in rat brain.
abstract: This study examined the immunolocalization and ontogeny of the inhibin-specific alpha subunit in the brain of male rats. Immunohistochemistry using antiserum directed against the mature region of porcine inhibin alpha (1-19, Tyr20) revealed positive reactions in process-bearing cells resembling astroglia in several regions, especially in the dorsal region of the third ventricle, medial and ventral arcuate nucleus, hippocampal dentate gyrus, and layers 1-3 of the cerebral cortex. Generally, inhibin alpha-positive cells in the limbic cortex had larger cell bodies and longer processes than those in the hypothalamus. These inhibin alpha-positive cells were verified to be positive for glial fibrillary acidic protein (GFAP), a differentiated astroglial marker, by double immunolabelling. The expression of inhibin alpha mRNA was higher in the brains of neonatal rats than in those of adult rats, as revealed by reverse transcription-competitive polymerase chain reaction, although the similar changes of immunoreactive inhibin alpha subunit in the brain was not observed. Orchiectomy did not affect expression of inhibin alpha mRNA in the hypothalamic area. This study suggests that inhibin-related peptide is produced by differentiated astrocytes, especially in the hypothalamic arcuate nucleus, the hippocampal dentate gyrus, and the cerebral cortex, and that the expression of inhibin alpha is regulated during brain development.
authors: Torii Nutrient-Stasis Project, ERATO, R & D Corp. of Japan, Yokohama.
authors: H
authors: H
authors: Fujimura
authors: K
authors: K
authors: Ohsawa
authors: M
authors: M
authors: Funaba
authors: T
authors: T
authors: Murata
authors: E
authors: E
authors: Murata
authors: M
authors: M
authors: Takahashi
authors: M
authors: M
authors: Abe
authors: K
authors: K
authors: Torii
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007265:Inhibins; D008297:Male; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D017931:DNA Primers; D005904:Glial Fibrillary Acidic Protein; D010455:Peptides; D012333:RNA, Messenger; C094377:inhibin-alpha subunit; D007265:Inhibins