title: Hyperoxia causes decreased expression of vascular endothelial growth factor and endothelial cell apoptosis in adult retina.
abstract: Mice or humans with photoreceptor degenerations experience permeability and dropout of retinal capillaries. Loss of photoreceptors results in decreased oxygen usage and thinning of the retina with increased oxygen delivery to the inner retina. To investigate the possibility that increased tissue oxygen plays a role in the vascular damage, we exposed adult mice to hyperoxia, which also increases oxygen in the retina. After 1, 2, or 3 weeks of hyperoxia, there was a statistically significant decrease in retinal vascular density that was not reversible, and endothelial cell apoptosis was demonstrated by TUNEL staining. Mice exposed to hyperoxia and mice with photoreceptor degeneration both showed decreased expression of VEGF in the retina. After complete or near-complete degeneration of photoreceptors, there was increased expression of VEGF in RPE cells, which may explain the association of photoreceptor degeneration and neovascularization in or around the RPE. Increased expression of VEGF in photoreceptors of transgenic mice failed to prevent hyperoxia-induced retinal capillary dropout. These data suggest that increased oxygen in the retina, either by increased inspired oxygen or by photoreceptor degeneration, results in endothelial cell death and dropout of capillaries. Decreased expression of VEGF may be a contributing factor, but the situation may be more complicated for mature retinal vessels than it is for immature vessels, because VEGF replacement does not rescue mature retinal vessels, suggesting that other factors may also be involved.
authors: Department of Ophthalmology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-9277, USA.
authors: H
authors: H
authors: Yamada
authors: E
authors: E
authors: Yamada
authors: S F
authors: SF
authors: Hackett
authors: H
authors: H
authors: Ozaki
authors: N
authors: N
authors: Okamoto
authors: P A
authors: PA
authors: Campochiaro
mesh_terms: D000818:Animals; D017209:Apoptosis; D004195:Disease Models, Animal; D016228:Endothelial Growth Factors / Q000235:genetics; D004730:Endothelium, Vascular / Q000473:pathology*; D015870:Gene Expression / Q000235:genetics; D018496:Hyperoxia / Q000209:etiology / Q000473:pathology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008222:Lymphokines / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains; D008822:Mice, Transgenic; D009389:Neovascularization, Pathologic / Q000235:genetics; D010100:Oxygen / Q000494:pharmacology; D020419:Photoreceptor Cells, Vertebrate / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D012160:Retina / Q000473:pathology*; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors
chemical_list: D016228:Endothelial Growth Factors; D008222:Lymphokines; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D042442:Vascular Endothelial Growth Factors; D010100:Oxygen