title: Immunopotentiating activity of nigerooligosaccharides for the T helper 1-like immune response in mice.
abstract: The immunopotentiating activity of nigerooligosaccharides (NOS), a mixture of nigerose, nigerosyl glucose and nigerosyl maltose, was studied in vitro and in vivo in mice. Mitogen-induced proliferation of splenocytes from normal mice was augmented in a dose-dependent manner by nigerose of NOS. NOS enhanced interleukin 12 (IL-12) and interferon-gamma (IFN-gamma) production by normal splenocytes in the presence of the potent IL-12 inducer, heat-killed Lactobacillus plantarum L-137, in vitro. Consistent with the in vitro finding, L. plantarum L-137-induced IL-12 production and IL-2-induced IFN-gamma production were augmented in mice fed with a 14.6% NOS diet for 2 weeks compared with mice fed with a control diet. Notably, mice fed with the NOS diet showed significantly longer survival time than the control mice after the induction of an endogenous infection by administering 5-fluorouracil in a lethal dose. Taken together, these results suggest that NOS may exert immunopotentiating activity through the activation of an IL-12-dependent T helper 1-like immune response.
authors: Research & Development Section, Takeda Food Products Ltd., Itami, Japan.
authors: S
authors: S
authors: Murosaki
authors: K
authors: K
authors: Muroyama
authors: Y
authors: Y
authors: Yamamoto
authors: H
authors: H
authors: Kusaka
authors: T
authors: T
authors: Liu
authors: Y
authors: Y
authors: Yoshikai
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002478:Cells, Cultured; D003208:Concanavalin A / Q000276:immunology; D004032:Diet / Q000662:veterinary; D004187:Disaccharides / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D004927:Escherichia coli Infections / Q000276:immunology; D005260:Female; D005472:Fluorouracil / Q000506:poisoning; D018928:Immunity, Mucosal; D007371:Interferon-gamma / Q000032:analysis / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis / Q000097:blood; D007778:Lactobacillus / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009844:Oligosaccharides / Q000276:immunology* / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D013154:Spleen / Q000166:cytology / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D004187:Disaccharides; D008070:Lipopolysaccharides; D009844:Oligosaccharides; C014637:laminaribiose; D003208:Concanavalin A; D018664:Interleukin-12; D007371:Interferon-gamma; D005472:Fluorouracil