title: NGF levels decrease in the spinal cord and dorsal root ganglion after spinal hemisection.
abstract: To examine changes in nerve growth factor (NGF) levels in spinal cord and dorsal root ganglia (DRG) after spinal injury, male Sprague-Dawley rats weighing 150-175 g were given spinal hemisections. NGF content was measured at various post-surgical times and compared with naive controls (n = 4 per time point) in the spinal cord, DRG and blood serum by ELISA techniques (Promega). Levels of NGF in the blood serum were significantly increased 8-fold at 48h but were significantly decreased in the spinal cord and DRG by 2- to 4-fold until 7 days postsurgery (ANOVA, p < 0.05). Contrary to accepted dogma, spinal injury results in decreased levels of NGF in the spinal cord and DRG following spinal injury.
authors: Marine Biomedical Institute and Department of Anatomy and Neurosciences, University of Texas Medical Branch, Galveston 77555-1069, USA.
authors: A D
authors: AD
authors: Bennett
authors: G
authors: G
authors: Taglialatela
authors: R
authors: R
authors: Perez-Polo
authors: C E
authors: CE
authors: Hulsebosch
mesh_terms: D000818:Animals; D002818:Cordotomy; D004797:Enzyme-Linked Immunosorbent Assay; D007839:Functional Laterality / Q000502:physiology; D005727:Ganglia, Spinal / Q000378:metabolism*; D008297:Male; D009414:Nerve Growth Factors / Q000097:blood / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors