Detection of foetal cells in maternal blood and prenatal sex determination by in situ hybridization. Procedure verification.
We describe an enrichment of foetal cells from maternal blood with a combination of double density gradient and Magnetic Activated Cell Sorting (MACS) of CD71, glycophorin A (GPA), CD34 and CD36 antibodies labeled cells followed by fluorescence in situ hybridization (FISH) with chromosome-specific DNA probes for determination of foetal sex.
Chiesa
J
J
Laboratoire de Biologie Cellulaire et de Cytogénétique Moléculaire, UPRES J.E 1952, Faculté de Médecine Montpellier-Nîmes, France.
Ferrer
C
C
Hoffet
M
M
Mares
P
P
Bureau
J P
JP
D000328:Adult; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods* / Q000592:standards; D008297:Male; D008875:Middle Aged; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods; D012732:Sex Determination Analysis / Q000379:methods*