title: Site-specific derivatization of RNA with photocrosslinkable groups.
abstract: Derivatization of RNA with heterobifunctional photocrosslinking reagents becomes an increasingly popular method for the analysis of structural properties of ribonucleoprotein complexes. This article describes a simple chemical modification-derivatization strategy used to introduce selected chemical groups at specific internal positions within the RNA ribose backbone. The strategy is based on the coupling of a haloacetyl adduct to a thiol residue in the phosphodiester bond. The use of a number of RNA probes derivatized with several different photoreactive groups can provide invaluable information on the structural distribution of components in complex ribonucleoprotein assemblies.
authors: Laboratory of Molecular Biology and Biochemistry, The Rockefeller University, New York, New York 10021, USA.
authors: M M
authors: MM
authors: Konarska
mesh_terms: D001386:Azides / Q000494:pharmacology; D001577:Benzophenones / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D008956:Models, Chemical; D019920:Photoaffinity Labels / Q000494:pharmacology; D012313:RNA / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000378:metabolism; D012266:Ribose / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D001386:Azides; D001577:Benzophenones; D003432:Cross-Linking Reagents; D019920:Photoaffinity Labels; D012313:RNA; D012266:Ribose; C047723:benzophenone; D012259:Ribonuclease, Pancreatic; C011171:4-azidophenacyl bromide