Rings and filaments of beta protein from bacteriophage lambda suggest a superfamily of recombination proteins.
The beta protein of bacteriophage lambda acts in homologous genetic recombination by catalyzing the annealing of complementary single-stranded DNA produced by the lambda exonuclease. It has been shown that the beta protein binds to the products of the annealing reaction more tightly than to the initial substrates. We find that beta protein exists in three structural states. In the absence of DNA, beta protein forms inactive rings with approximately 12 subunits. The active form of the beta protein in the presence of oligonucleotides or single-stranded DNA is a ring, composed of approximately 15-18 subunits. The double-stranded products of the annealing reaction catalyzed by the rings are bound by beta protein in a left-handed helical structure, which protects the products from nucleolytic degradation. These observations suggest structural homology for a family of proteins, including the phage P22 erf, the bacterial RecT, and the eukaryotic Rad52 proteins, all of which are involved in homologous recombination.
Passy
S I
SI
Department of Cell Biology and Neuroanatomy, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Yu
X
X
Li
Z
Z
Radding
C M
CM
Egelman
E H
EH
D010582:Bacteriophage lambda / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*; D046911:Macromolecular Substances; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000648:ultrastructure; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure*
D004279:DNA, Viral; D004268:DNA-Binding Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D014764:Viral Proteins; C118730:beta protein, Bacteriophage lambda; D004247:DNA