title: Mucosal mast cell responses and release of mast cell protease-I in infections of mice with Hymenolepis diminuta and H. microstoma: modulation by cyclosporin A.
abstract: The dynamics of intestinal mucosal mast cells and the major mucosal mast cell protease were followed during the course of laboratory infections of mice with Hymenolepis diminuta and H. microstoma. The effects of the drug cyclosporin A (CsA), which is both immunosuppressive and selectively anthelmintic depending upon dose regime, were determined. In H. diminuta infections worm expulsion occurred around day 9 and coincided with peak mastocytosis and peak mMCP-I concentrations in tissues and serum. Immunosuppressive treatment with CsA prevented worm expulsion, permitting some individuals to reach maturity, and abrogated mast cell proliferation and mMCP-I production and release. By contrast, H. microstoma infections persisted for 64 days in spite of a considerable mastocyosis in both intestine and bile duct tissues accompanied by a high level of mMCP-I in tissues and serum. A subimmunosuppressive regime of CsA had only limited effects on worms and mast cell numbers and activity. Together these data shed light on the variable mast cell response to gastrointestinal infections and on the potential significance of parasite location in evasion of mast cell action. Use of CsA reveals the contributions of both T cell-dependent mechanisms, including mast cell proliferation and activation, and T cell-independent events in regulating intestinal helminth infections.
authors: Department of Zoology, University of Aberdeen, Scotland, UK.
authors: P E
authors: PE
authors: McLauchlan
authors: H C
authors: HC
authors: Roberts
authors: N J
authors: NJ
authors: Loxton
authors: J M
authors: JM
authors: Wastling
authors: G F
authors: GF
authors: Newlands
authors: L H
authors: LH
authors: Chappell
mesh_terms: D000818:Animals; D001652:Bile Ducts; D002455:Cell Division; D053818:Chymases; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D006925:Hymenolepiasis / Q000276:immunology*; D006926:Hymenolepis / Q000276:immunology*; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000166:cytology; D007422:Intestines / Q000166:cytology; D008407:Mast Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008808:Mice, Inbred CBA; D012697:Serine Endopeptidases / Q000378:metabolism*; D014231:Tribolium
chemical_list: D007166:Immunosuppressive Agents; D016572:Cyclosporine; D012697:Serine Endopeptidases; D053818:Chymases