Modulation by nitric oxide (NO) of capsaicin-induced calcium uptake into rat dorsal root ganglion neurons.
The regulatory role of nitric oxide in capsaicin-induced 45Ca2+ accumulation in dorsal root ganglion neuronal cultures was investigated. Capsaicin-activated calcium entry was subject to complicated tuning by NO-releasing agents sodium nitroprusside, spermine/NO complex and NO synthase inhibitor NG-nitro-L-arginine methyl ester in concentration and stimulation protocol-dependent manner. In contrast, these agents failed to change depolarization-induced calcium influx. In experiments using dithiothreitol and 5,5-dithio-bis-2-nitrobenzoic acid this modulation was independent of the oxidizing action of NO. It is suggested that NO exerts a novel feedback modulatory effects on capsaicin-induced calcium entry into rat DRG neurons.
Park
Y H
YH
R&D Center, Pacific Co., Yongin, Korea.
Surh
Y J
YJ
Lee
S S
SS
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002211:Capsaicin / Q000031:analogs & derivatives / Q000494:pharmacology*; D004228:Dithionitrobenzoic Acid / Q000378:metabolism / Q000494:pharmacology; D004229:Dithiothreitol / Q000378:metabolism; D005727:Ganglia, Spinal / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009474:Neurons / Q000187:drug effects; D009543:Nifedipine / Q000494:pharmacology; D009569:Nitric Oxide / Q000494:pharmacology*; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology
D009599:Nitroprusside; D013096:Spermine; D009569:Nitric Oxide; D004228:Dithionitrobenzoic Acid; D014700:Verapamil; D009543:Nifedipine; C071423:capsazepine; D002211:Capsaicin; D002118:Calcium; D004229:Dithiothreitol; D019331:NG-Nitroarginine Methyl Ester