The golden age of retinal cell culture.
In the late 1950s, the study of retinal cells in vitro was in its infancy. Today, retinal cell and tissue culture is routinely used for studies of cell growth, differentiation, cytotoxicity, gene expression, and cell death. This review discusses the major classifications of retinal cell and tissue culture, including primary cell/explant models, retinoblastoma cell lines, and genetically engineered cell lines. These topics are addressed in an historical perspective, coupled with present-day applications for this continually-developing technology.
Seigel
G M
GM
Department of Neurobiology & Anatomy and Center for Visual Science, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA. gail_seigel@urmc.rochester.edu
D000818:Animals; D018929:Cell Culture Techniques / Q000266:history / Q000379:methods; D002454:Cell Differentiation; D002460:Cell Line; D046508:Culture Techniques / Q000266:history / Q000379:methods; D005818:Genetic Engineering / Q000266:history; D049673:History, 20th Century; D006801:Humans; D012160:Retina / Q000166:cytology*; D012175:Retinoblastoma / Q000266:history / Q000473:pathology; D014407:Tumor Cells, Cultured