Short communication: lack of stage-specific embryonic antigen-1 expression by bovine embryos and primordial germ cells.
The objective of this study was to determine whether stage-specific embryonic antigen-1, a cellular marker commonly used to identify murine undifferentiated embryonic cells, is also a useful marker for bovine pluripotent cells. Expression of stage-specific embryonic antigen-1 was examined by indirect immunohistochemistry on bovine preimplantation embryos and on primordial germ cells contained in the genital ridge. Expression of stage-specific embryonic antigen-1 was not observed in any of the cleavage-stage bovine embryos examined, including one-cell, two-cell, four-cell, eight-cell, morula, and blastocyst stages, nor in tissue sections of bovine genital ridges collected from embryos on d 34, 37, and 40 of gestation. As expected, expression of stage-specific embryonic antigen-1 was detected on murine preimplantation embryos and on murine teratocarcinoma cells. Results of this study indicate that, unlike in the mouse, stage-specific embryonic antigen-1 is not a useful cellular marker for pluripotent bovine embryonic cells or bovine primordial germ cells.
Choi
S J
SJ
Department of Animal Science, University of California, Davis 95616, USA.
Shim
H
H
Anderson
G B
GB
D000818:Animals; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers; D002417:Cattle / Q000196:embryology*; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000737:chemistry*; D005314:Embryonic and Fetal Development; D019084:Fluorescent Antibody Technique, Indirect; D015870:Gene Expression; D005854:Germ Cells / Q000737:chemistry*; D016256:Lewis X Antigen / Q000032:analysis*; D051379:Mice; D009028:Morula / Q000737:chemistry; D013997:Time Factors
D000911:Antibodies, Monoclonal; D015415:Biomarkers; D016256:Lewis X Antigen