title: Effects of Aspergillus fumigatus culture filtrate on antifungal activity of human phagocytes in vitro.
abstract: BACKGROUND
  Aspergillus fumigatus can colonise the airways and the lungs with localised underlying conditions and occasionally invade the surrounding lung tissues even in subjects without systemic predisposing factors, presumably by escaping the local host defences. The aim of this study was to investigate the effects of A fumigatus culture filtrate (ACF) on the activities of human phagocytes--inhibition of germination of A fumigatus spores by alveolar macrophages (AMs) and hyphal damage by polymorphonuclear leucocytes (PMNs)--which are the critical host defences against A fumigatus.
  
  
  METHODS
  Spores were incubated with AMs at a ratio of 1:1 in a medium containing different concentrations of ACF for 10 hours at 37 degrees C. Spore germination was visualised with light microscopy and the inhibition rate was calculated. The percentage of hyphal damage caused by PMNs pretreated with various concentrations of ACF was measured by a colorimetric tetrazolium metabolic assay.
  
  
  RESULTS
  The inhibition rate of spore germination by AMs cultured with medium alone (control) was 90 (0.8)% whereas that by AMs cultured with the medium containing 10% ACF was significantly (p < 0.05) reduced to 41.7 (4.6)%. ACF suppressed the inhibition of spore germination in a dose dependent manner without altering the phagocytosing activity against the spores. The percentage of hyphal damage caused by PMNs pretreated with medium-199 (control) was 78.1 (2.3)% compared with 65.3 (2.8)% when PMNs were pretreated with 50% ACF (p < 0.05).
  
  
  CONCLUSIONS
  A fumigatus releases biologically active substance(s) which suppress the inhibition of spore germination by AMs and also suppress PMN mediated hyphal damage, and thus may contribute to the pathogenicity of this fungus.
authors: Department of Infection and Inflammation, Kyoto University, Japan.
authors: T
authors: T
authors: Murayama
authors: R
authors: R
authors: Amitani
authors: Y
authors: Y
authors: Ikegami
authors: R
authors: R
authors: Kawanami
authors: W J
authors: WJ
authors: Lee
authors: R
authors: R
authors: Nawada
mesh_terms: D000328:Adult; D001232:Aspergillus fumigatus / Q000276:immunology* / Q000378:metabolism; D001685:Biological Factors / Q000494:pharmacology*; D018929:Cell Culture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D005374:Filtration; D006801:Humans; D016676:Macrophages, Alveolar / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D010100:Oxygen / Q000378:metabolism; D010587:Phagocytosis; D016897:Respiratory Burst
chemical_list: D001685:Biological Factors; D017077:Culture Media, Conditioned; D010100:Oxygen