title: Induction of interleukin-10 on activation of Epstein-Barr virus in EBV-infected B-cell lines.
abstract: Human (h) interleukin-10 (IL-10) exhibits a strong DNA and amino acid sequence homology to the Epstein-Barr virus (EBV) BCRF1 genome, viral (v) IL-10. We analyzed the production of IL-10 for EBV activation in B-cell lines. The latent EBV in Akata cells was activated by the cross-linking of surface immunoglobulin G (IgG) with anti-human IgG. The levels of IL-10(h+v) and vIL-10 in the culture fluids were measured by a specific enzyme-linked immunosorbent assay (ELISA). IL-10(h+v) was detected at the same time for EBV immediate early gene BZLF1 product ZEBRA and early gene BMRF1 product EA-D. This was more than 4 hours prior to the appearance of vIL-10, and late gene products gp 350/220 and viral capsid antigen. The induction of hIL-10 and vIL-10 mRNAs were detected in anti-IgG-treated Akata cells by reverse transcription-polymerase chain reaction. The induction of IL-10(h+v) and vIL-10 was inhibited with a tyrosine kinase inhibitor, herbimycin, or with an inhibitor of herpesvirus DNA polymerase, phosphonoacetic acid, or acyclovir. IL-10(h+v) and vIL-10 were also detected in the supernatants of Akata and Daudi but not Ramos cells infected with P3HR-1 EBV. These results show the IL-10 induction on EBV activation in EBV-carrying B-cell lines.
authors: Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
authors: T
authors: T
authors: Sairenji
authors: E
authors: E
authors: Ohnishi
authors: S
authors: S
authors: Inouye
authors: T
authors: T
authors: Kurata
mesh_terms: D000212:Acyclovir / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects / Q000821:virology*; D002460:Cell Line; D015870:Gene Expression; D004854:Herpesvirus 4, Human / Q000254:growth & development*; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology; D016753:Interleukin-10 / Q000096:biosynthesis* / Q000235:genetics; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors; D012333:RNA, Messenger; D014775:Virus Activation
chemical_list: D000998:Antiviral Agents; D007074:Immunoglobulin G; D012333:RNA, Messenger; D016753:Interleukin-10; D011505:Protein-Tyrosine Kinases; D010746:Phosphonoacetic Acid; D000212:Acyclovir