Preventive effects of a mixed disulphide from dithiocarbamate and N-acetylcysteine on the genotoxicity of N-nitrosodiethylamine.
A mixed disulphide model compound, S-(N,N-diethyldithiocarbamoyl)-N-acetylcysteine (AC-DDTC), prepared from diethyldithiocarbamate and N-acetylcysteine, was investigated for protective effects against the genotoxicity of the environmental carcinogen N-nitrosodiethylamine (NDEA). AC-DDTC was found to be a potent inhibitor of genotoxicity induced by NDEA. The mutagenicity of NDEA to Salmonella typhimurium was inhibited by 70% at 3.2 micromol AC-DDTC per plate and the effect was dose-dependent. In the chromosome aberration assay, NDEA elicited a significant increase in the number of aberrant cells. Pretreatment with AC-DDTC suppressed the chromosome-damaging effect of NDEA. The micronucleus-inducing capacity of NDEA was reduced by 32% by treatment with AC-DDTC at 1.5 mmol kg(-1). These results suggest that AC-DDTC might have a role to play in reducing the risk of cancer induced by NDEA.
Lee
B H
BH
College of Pharmacy, Wonkwang University, Iksan, Jeonbuk, Korea.
Lee
S J
SJ
D000111:Acetylcysteine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002869:Chromosome Aberrations / Q000235:genetics; D006224:Cricetinae; D003412:Cricetulus; D004052:Diethylnitrosamine / Q000633:toxicity*; D004347:Drug Interactions; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D012486:Salmonella typhimurium / Q000235:genetics
D016587:Antimutagenic Agents; D009153:Mutagens; C098444:S-(N,N-diethyldithiocarbamoyl)-N-acetylcysteine; D004052:Diethylnitrosamine; D000111:Acetylcysteine