Selection analysis of single-stranded clones using solid-phase techniques.
An efficient selection analysis of single-stranded DNA fragments using subtractive hybridization on magnetic beads and fluorescence-based fragment analysis is described. This approach facilitates either direct cloning of captured fragments or removal of common clones from cDNA or shot-gun clone libraries.
Vener
T
T
KTH, Royal Institute of Technology, Department of Biotechnology, Stockholm, Sweden. tvener@twt.com
Holmberg
A
A
Lundeberg
J
J
Uhlén
M
M
D000818:Animals; D004277:DNA, Single-Stranded / Q000032:analysis*; D006801:Humans; D051379:Mice; D009693:Nucleic Acid Hybridization / Q000379:methods*
D004277:DNA, Single-Stranded