title: Myophilin of Echinococcus granulosus: isoforms and phosphorylation by protein kinase C.
abstract: Myophilin is a muscle-associated antigen of the taeniid cestode Echinococcus granulosus. This protein shows a high amino acid sequence homology with calponins and calponin-like proteins, which are proposed to be associated with the regulation of smooth muscle contraction. In order to provide supportive evidence for a relationship between these proteins, we characterized myophilin using electrophoretic, biochemical and molecular biological approaches. Two-dimensional protein electrophoretic separation of E. granulosus larval proteins defined 4 isoelectric isoforms of myophilin (alpha, beta, gamma and delta), which appeared to be a consequence of post-translational modification of a single gene product. It was also demonstrated biochemically that E. granulosus myophilin undergoes specific phosphorylation in vitro by protein kinase C (PKC). Finally, myophilin homologues were identified in extracts of Taenia hydatigena and T. ovis by immunoblot. A partial cDNA of the closely related species, E. multilocularis, was isolated by cloning procedures and showed 99% homology with the E. granulosus myophilin gene. The similarities of E. granulosus myophilin with calponins in their tissue localization, protein isoforms patterns, ability to be phosphorylated in vitro by PKC, and the relatively conserved nature of the protein among related parasites suggest that myophilin may be associated with smooth muscle contraction.
authors: University of Melbourne, Department of Veterinary Science, Werribee, Victoria, Australia. martin@wehi.edu.au
authors: R M
authors: RM
authors: Martin
authors: X F
authors: XF
authors: Csar
authors: R B
authors: RB
authors: Gasser
authors: R
authors: R
authors: Felleisen
authors: M W
authors: MW
authors: Lightowlers
mesh_terms: D000818:Animals; D000947:Antigens, Helminth; D015152:Blotting, Northern; D018509:DNA, Helminth; D004443:Echinococcosis / Q000469:parasitology; D004446:Echinococcus / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015151:Immunoblotting; D009124:Muscle Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D020033:Protein Isoforms; D011493:Protein Kinase C / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D000947:Antigens, Helminth; D018509:DNA, Helminth; D015801:Helminth Proteins; D009124:Muscle Proteins; D020033:Protein Isoforms; D011994:Recombinant Proteins; C095050:myophilin protein, Echinococcus granulosus; D011493:Protein Kinase C