A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07.
Arabidopsis thaliana BAC T07M07 encoding the abscisic acid-insensitive 4 (ABI4) locus has been sequenced completely. It contains a 95,713-bp insert and 24 predicted genes. Most putative genes were confirmed by gel-based RNA profiling and a cluster of ABA-regulated genes was identified. One of the 24 genes, designated PP2C5, encodes a putative protein phosphatase 2C. The encoded protein was expressed in Escherichia coli, and its enzyme activity in vitro was confirmed.
Wang
M L
ML
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA.
Belmonte
S
S
Kim
U
U
Dolan
M
M
Morris
J W
JW
Goodman
H M
HM
D000040:Abscisic Acid / Q000378:metabolism; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D015152:Blotting, Northern / Q000379:methods; D002876:Chromosomes, Bacterial; D003001:Cloning, Molecular / Q000379:methods; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics*; D010749:Phosphoprotein Phosphatases / Q000235:genetics; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C; D029701:Saccharomyces cerevisiae Proteins; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D029701:Saccharomyces cerevisiae Proteins; D000040:Abscisic Acid; C087358:PTC1 protein, S cerevisiae; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2; D000071636:Protein Phosphatase 2C