title: Sites of simian foamy virus persistence in naturally infected African green monkeys: latent provirus is ubiquitous, whereas viral replication is restricted to the oral mucosa.
abstract: Foamy viruses (FV), retroviruses of the genus Spumavirus, are able to infect a wide variety of animal species and replicate in nearly all types of cultured cells. To identify the cells targeted by FV in the natural host and define the sites of viral replication, multiple organs of four African green monkeys naturally infected with simian FV type 3 were investigated for the presence of FV proviral DNA and viral transcripts. All organs contained significant amounts of FV proviral DNA. In addition to proviruses containing the complete transactivator gene taf, proviral genomes carrying a specific 295-bp deletion in the taf gene were detected in all monkeys. As in the case of human foamy virus the deletion leads to the formation of the bet gene that is regarded to be instrumental in the regulation of viral persistence. FV RNA was detected by RT-PCR and in situ hybridization only in the oral mucosa of one monkey. No other samples contained detectable levels of viral transcripts. Histopathological changes were not observed in any of the tissue samples analyzed. Our results show that the natural history of FV is characterized by latent infection in all organs of the host and by minimal levels of harmless viral replication in the oral mucosa. The broad host cell range in vivo further encourages the development of FV-derived vectors for therapeutic gene delivery.
authors: Institute for Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany.
authors: V
authors: V
authors: Falcone
authors: J
authors: J
authors: Leupold
authors: J
authors: J
authors: Clotten
authors: E
authors: E
authors: Urbanyi
authors: O
authors: O
authors: Herchenröder
authors: W
authors: W
authors: Spatz
authors: B
authors: B
authors: Volk
authors: N
authors: N
authors: Böhm
authors: A
authors: A
authors: Toniolo
authors: D
authors: D
authors: Neumann-Haefelin
authors: M
authors: M
authors: Schweizer
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops / Q000821:virology*; D004279:DNA, Viral / Q000032:analysis; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D017403:In Situ Hybridization; D009061:Mouth Mucosa / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011533:Proviruses; D012367:RNA, Viral / Q000032:analysis; D012191:Retroviridae Proteins / Q000235:genetics; D016092:Spumavirus / Q000472:pathogenicity / Q000502:physiology*; D015534:Trans-Activators / Q000235:genetics; D017735:Virus Latency; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004268:DNA-Binding Proteins; D012367:RNA, Viral; D012191:Retroviridae Proteins; D015534:Trans-Activators; C074061:bel1 protein, Human foamy virus