Rapid anti-helminthic response of B lymphocytes in the intestinal mucosal tissues of rats.
B cell response to Trichinella spiralis (Ts) adult antigen (Ag) was studied in rats 1-20 days postinfection. B cell recoveries from the mesenteric lymph node (MLN), Peyer's patches (PP), thoracic duct lymph (TDL), and the spleen were determined by FACS analysis and Ag-specific antibody-producing cells (Ab-pc) in these tissues were enumerated using the immunoplaque assay. Total B cell numbers increased 2-70 times from day 3 postinfection in the MLN and TDL obtained from MLN-resected rats (MX) and such proliferation was not found in the PP or the spleen. Ab-pc of all isotypes increased from day 3 in the MLN and from day 2 in the MX-TDL. Among all isotypes, IgE- and IgG1-pc showed the strongest response. Immunofluorescence study revealed that these B cells were activated in the non-PP region of the small intestine. These results indicate an early isotype switch to IgG1 and IgE production in Ts-infected small intestine.
Wang
C H
CH
School of Natural Sciences, California State University, 5500 University Parkway, San Bernardino, California, 92407, USA.
Richards
E M
EM
Bell
R G
RG
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis*; D000921:Antibody-Producing Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000145:classification; D007413:Intestinal Mucosa / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D010581:Peyer's Patches / Q000276:immunology; D051381:Rats; D013154:Spleen / Q000276:immunology; D017160:Trichinella spiralis / Q000276:immunology*
D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D007073:Immunoglobulin E