Cell death and apoptosis regulating proteins in Parkinson's disease--a cautionary note.
We studied the substantia nigra of three Parkinson's disease (PD) patients and three age-matched individuals by in situ DNA-end labeling (ISEL) and immunohistochemistry for the apoptosis regulating proteins Bcl-2, Bax and Bcl-x on 50 consecutive sections per patient. No melanin-containing cell was identified with typical apoptotic changes in either patient or control substantia nigra. With prolonged reaction-time the terminal transferase-mediated DNA-end labeling revealed a signal in 2.0 +/-1.2% melanin-containing cells in PD compared to 1.3 +/-1.1% in control. This difference did nor reach statistical significance and no condensation or margination of the chromatin was evident. No significant changes of any of the apoptosis regulating proteins were apparent in PD substantia nigra. These findings do not support the hypothesis that apoptosis plays a central role in the pathogenesis of PD.
Wüllner
U
U
Department of Neurology, Friedrich-Wilhelms-University, Bonn, Germany. wuellner@uni-bonn.de
Kornhuber
J
J
Weller
M
M
Schulz
J B
JB
Löschmann
P A
PA
Riederer
P
P
Klockgether
T
T
D000199:Actins / Q000378:metabolism; D000368:Aged; D017209:Apoptosis; D002466:Cell Nucleolus / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D016631:Lewy Bodies / Q000473:pathology; D008543:Melanins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010300:Parkinson Disease / Q000378:metabolism / Q000473:pathology*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D013378:Substantia Nigra / Q000378:metabolism* / Q000473:pathology; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein
D000199:Actins; C494826:BAX protein, human; C494811:BCL2L1 protein, human; D008543:Melanins; D011518:Proto-Oncogene Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein