Oxidation of medium-chain acyl-CoA esters by extracts of Aspergillus niger: enzymology and characterization of intermediates by HPLC.
The activities of beta-oxidation enzymes were measured in extracts of glucose- and triolein-grown cells of Aspergillus niger. Growth on triolein stimulated increased enzyme activity, especially for acyl-CoA dehydrogenase. No acyl-CoA oxidase activity was detected. HPLC analysis after incubation of triolein-grown cell extracts with decanoyl-CoA showed that beta-oxidation was limited to one cycle. Octanoyl-CoA accumulated as the decanoyl-CoA was oxidized. Beta-oxidation enzymes in isolated mitochondrial fractions were also studied. The results are discussed in the context of methyl ketone production by fungi.
Baltazar
M Fätima
MF
Dickinson
F Mark
FM
Ratledge
Colin
C
D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism; D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism; D000214:Acyl Coenzyme A / Q000378:metabolism*; D042964:Acyl-CoA Dehydrogenase; D044944:Acyl-CoA Dehydrogenases / Q000378:metabolism; D042962:Acyl-CoA Oxidase; D000438:Alcohols / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism; D002458:Cell Fractionation; D002851:Chromatography, High Pressure Liquid; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004746:Enoyl-CoA Hydratase / Q000378:metabolism; D004789:Enzyme Activation; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007659:Ketones / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D014304:Triolein / Q000378:metabolism
D000214:Acyl Coenzyme A; D000438:Alcohols; D004952:Esters; D005227:Fatty Acids; D007659:Ketones; D014304:Triolein; D010088:Oxidoreductases; D015094:3-Hydroxyacyl CoA Dehydrogenases; D044944:Acyl-CoA Dehydrogenases; D042962:Acyl-CoA Oxidase; D042964:Acyl-CoA Dehydrogenase; D000102:Acetyl-CoA C-Acyltransferase; D002332:Carnitine O-Acetyltransferase; D002950:Citrate (si)-Synthase; D004746:Enoyl-CoA Hydratase; D005947:Glucose