[THe use of a biotin-labelled DNA probe for detecting the causative agents of chronic urogenital infections].
DNA hybridization for detecting HSV, CMV, C. trachomatis, and U. urealyticum by biotin-labeled DNA probe was used for investigating clinical specimens from patients with infertility and chronic urogenital inflammations. High sensitivity and specificity of the method was confirmed by the results of PCR, ELISA, and immunofluorescent methods in 80-90% cases. DNA hybridization technique is a simple method requiring no sophisticated equipment, which recommends it for the diagnosis of sexually-transmitted diseases at clinical laboratories.
Kvasov
A V
AV
Zhdanov
A V
AV
Pis'menskaia
I V
IV
Faĭzullina
N M
NM
Faĭzullin
L Z
LZ
Sukhikh
G T
GT
D001710:Biotin; D002908:Chronic Disease; D015342:DNA Probes; D004269:DNA, Bacterial / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004847:Epithelial Cells / Q000382:microbiology / Q000821:virology; D005260:Female; D006801:Humans; D015151:Immunoblotting / Q000295:instrumentation / Q000379:methods; D008297:Male; D009693:Nucleic Acid Hybridization / Q000379:methods; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014552:Urinary Tract Infections / Q000175:diagnosis* / Q000382:microbiology / Q000821:virology
D015342:DNA Probes; D004269:DNA, Bacterial; D004279:DNA, Viral; D001710:Biotin