Activation of spleen lymphocytes by plasmid DNA.
Plasmid pUC19 DNA was shown to stimulate in vitro proliferation of CBA mouse splenocytes in a dose-dependent manner. Simultaneous treatment of the cells with the plasmid DNA and Con A or LPS produced an additive effect, while PMA acted synergistically with DNA. Monovalent Fab fragments of rabbit anti-mouse Ig (RAMIg) antibodies significantly inhibited plasmid DNA-induced polyclonal lymphocyte activation suggesting the involvement of Ig receptors in this process. Affinity modification of lymphocytes membrane-cytosole proteins with a 32P-labeled alkylating oligonucleotide derivative resulted in labeling of 67-82 and 23 kDa polypeptides corresponding to IgD and IgM heavy and light chains respectively. The immunoglobulin nature of the 82 and 23 kDa oligonucleotide-binding polypeptides was confirmed by immunoprecipitation with RAMIg antibodies.
Rykova
E Y
EY
Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk. rykova@modul.bioch.nsk.su
Laktionov
P P
PP
Vlassov
V V
VV
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D004247:DNA / Q000494:pharmacology*; D004268:DNA-Binding Proteins / Q000378:metabolism; D007140:Immunoglobulin Fab Fragments / Q000494:pharmacology; D007128:Immunoglobulin Fragments / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010957:Plasmids / Q000235:genetics*; D011817:Rabbits; D011971:Receptors, Immunologic / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D004268:DNA-Binding Proteins; D007140:Immunoglobulin Fab Fragments; D007128:Immunoglobulin Fragments; D011971:Receptors, Immunologic; D004247:DNA