Preoperative treatment of rectal cancer with radiation, chemotherapy and hyperthermia: analysis of treatment efficacy and heat-shock response.
Preoperative treatment of locally advanced rectal cancer with radiation, chemotherapy and hyperthermia is analyzed with regard to heat-shock response. In 23 patients with locally advanced rectal cancer (uT3/uT4), hyperthermia was administered in combination with radiotherapy and chemotherapy. In parallel, the effect of the treatment on levels of the heat-shock proteins HSP27 and inducible HSP70 in tumors and surrounding tissues was investigated by Western blotting. The patients' sera were also examined for autoantibodies against HSPs. HSP27 and inducible HSP70 were detected in most rectal tumors and surrounding tissues before and after treatment. HSP27 and inducible HSP70 levels had changed in 10 tumors after treatment. However, prior to treatment, there existed an unexpected diversity in HSP levels in the tumors and surrounding tissue. Hyperthermia doses in cumulative minutes for which 90% of the tumor is above the reference temperature (cum min T90 > or = 15 min) led to increased survival and response compared to that of a control group of patients treated without or with low-dose hyperthermia (cum min T90 < 15 min). However, there was no correlation to different expression of the HSPs. Hyperthermia as used in this setting does not lead to any sustained expression of HSPs in either the tumor or the surrounding tissue.
Rau
B
B
Division of Surgery and Surgical Oncology, Robert Rössle Hospital and Tumor Institute, Berlin-Buch, Berlin, Germany.
Gaestel
M
M
Wust
P
P
Stahl
J
J
Mansmann
U
U
Schlag
P M
PM
Benndorf
R
R
D000906:Antibodies / Q000097:blood; D003131:Combined Modality Therapy; D018572:Disease-Free Survival; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000096:biosynthesis; D006360:Heat-Shock Proteins / Q000096:biosynthesis*; D006801:Humans; D006979:Hyperthermia, Induced; D008297:Male; D012004:Rectal Neoplasms / Q000378:metabolism / Q000401:mortality / Q000628:therapy*
D000906:Antibodies; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins